LOCUS       DATACA010000001        15758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2490149, whole genome shotgun sequence.
ACCESSION   DATACA010000001 DATACA010000000
VERSION     DATACA010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15758
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490149"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..932
                     /locus_tag="VHK28_00005"
     CDS             105..932
                     /locus_tag="VHK28_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2140644.1"
                     /translation="MTLGETETTIRQRRQFAEQAISQATRGQWEEAVESNRQLLELGP
                     DTDAYNRLGKALAELGRHAEALEAYQQALARDATNRIAERNVARLEVLLGGDKGDRDN
                     GRTGKASAASFIEEMGKTGHARLINLAPARQLAPLSPGDAVELVLDGEMLVAMAGDVA
                     VGQVEPRVGSRLAKLMKGGNRYEAAITVLDRDESRIIIREVFAHPDNFGKVSFPGSAT
                     GRAGDVRPYMKGSALRYDDDEESEEQDEEQEEVEELDTTLPEYSAEPDLEEELLEEP"
     gene            941..3787
                     /locus_tag="VHK28_00010"
     CDS             941..3787
                     /locus_tag="VHK28_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140645.1"
                     /translation="MGEAGRRRLDHVVLPRRLPGLALLERYFPSPADNQLDAELSAFA
                     RAGDTVLDPWAGTGWTARRAIAHGLRAVSADPSPFAQLAAQAFLLAPDPSAIDSAFNQ
                     LASSRRVDVPLRQHIEELYASRCAVCRHPVVGDQFIWPRDADAPGRKIYRCSNCEISV
                     GGAEERVAPVDEVDLAKLGIERPSDSRADIESDPSIDAEDDLPAAPVGLTGVETGTSV
                     AAYDPADHSARDALGEPGEPPPPPAITPDAPTGPGSGQGPGPRFASTVRPDPLPPQAA
                     TEVRKGPHYQQLRDRFPVLDGQSALVDELLELYTPRNLYALQTIANKIDAEFREPALS
                     AVFRVALAACLLPASRLNGYPGRVASLRVSGGHVRQPASRYQREVNVWRLFEAAVRDV
                     RTAIAALGRDRRPARFAAGLEDLGGVSAANVLWIRCRPAVIGQYLPEEGVDLVLTSIA
                     ASGSVDEISFEYLATAWLIGREAAETLRLEPLFGSGPARSGAAEATALRHAIASAAGA
                     LKPDGWFVVILEGSDVDRLLSVAVAGAAGGLDLVDVIHRESRRSGEGVTLHFHKPSAE
                     DRLRAAVQPKPLRLGAEDGRLTYPELAEAIDQASVGLLRTRGEPAGLIRMVAAVLLEL
                     QRTDLLRRVAQARPNGEAAEPSDAELEGRIERGGGLLGSLLREELERDDHPTLVRLGE
                     GGGSMWWLRDPEIADSPLADRVEWSTFSVLSTAGRLDADGFLERIYALFPGLDSPDEE
                     LVRACLAAYGRTGDGGLLRTDDDLGRRLEEHSAVIAALVDYAHRSGLRTWVSRREQHR
                     VVEGRTLEERLLEDERRAYLPLIIRAPAEVVGAVDAIWYVRGRLALLFEVEWTAMLGE
                     PILRRGRQIPVGDEQARFLVFPAERTELVRLKLERSPWLRAEVERQNWHFLKWQHLHA
                     LVAGEGAGLERLEPFLGLDPLAERGGQQLTMFGE"
     gene            complement(3905..4246)
                     /locus_tag="VHK28_00015"
     CDS             complement(3905..4246)
                     /locus_tag="VHK28_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013353610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX2140646.1"
                     /translation="MRSSREAHFRHAYTKDRQELLNRLRRIEGQARGIQRMVESEAYC
                     LEILQQVDAMTAAADQVSMLVLEDHIAGCLAHAIESGQGQPYVDEVMTVVRRAVGRPA
                     TGKRATRSDGG"
     gene            4278..5006
                     /locus_tag="VHK28_00020"
     CDS             4278..5006
                     /locus_tag="VHK28_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX2140647.1"
                     /translation="MTDVERHELITPLERVLVIFSHPDDAEFGAAPTIAALARLGARV
                     DYLVTTDGGKGTDDPAVTPQQLAATRRAEQRAAADVLGVSEIVHLDYPDGYLTPSLDL
                     RRDISRQIRRFRPDLVIAQNPQRRSDGNPFIAHPDHLATGEATLAAVYPAARDRLNFP
                     ELIDEGLEPWKVRQVLVTGVERPNLWIDVGETLETGMRALRCHVSQVDDWEAVETRIL
                     ERTAEAGRAVALERAQAFLSILLQ"
     gene            5003..6901
                     /locus_tag="VHK28_00025"
     CDS             5003..6901
                     /locus_tag="VHK28_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2140648.1"
                     /translation="MTRRGHWGGGPEPDLTARPGSGPRTLRLIAGFFRPYRFRLGLIS
                     VLILVTVSVGVVNPILLKLIIDNLLGPQDLGLLYLQVALMVVLPVVSSGIGVWQSFLS
                     NVVGQRVMDDLRLALYTHLQWMPLRFFTETRTGEIQSRIGNDVNGVQSVVTDTAASLL
                     SNFATVATTVIAMLILDWRLTILSLGMLPVFAYITFRVGKVRREVSGMTQRSLAEVSA
                     ITEETLSVSGVLLSKTFGQQRASVERFRRESRRLGDLQIRQQMIGRWFFAMIGTFFSI
                     APAFVYLLAGSLIIGGDTTVSVGTIVAFTTLQSRLFFPLGQLLNVQVEIQGALALFDR
                     IFEYLEMPHEITDAPDAVDLTTANTRGEVRFRGVSFRYPVSPGQVQAQAEAELAEATG
                     RAVHLPIQPFGLTDIDFTARPGELVALVGPSGSGKTTTTYLVPRLYDVDTGSVEIDGH
                     DVRGIRLESLGRVIGVVTQETYLFHASVLDNLRYAKPEAIMDEVEAAARAAAIHDRIL
                     ELPEGYDTIVGERGYKLSGGEKQRVAIARVLLKDPRILILDEATSALDTVSERLIQAA
                     LNRLMQGRTTIAIAHRLSTILRADQILVFDRGRIVERGTHDELLRHEGLYARLYAEQF
                     AAEQPVPR"
     gene            7201..9285
                     /locus_tag="VHK28_00030"
     CDS             7201..9285
                     /locus_tag="VHK28_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2140649.1"
                     /translation="MTDASPPRHTNRLAAETSPYLLQHAHNPVDWYPWGQDALARARL
                     LDRPIFLSIGYSACHWCHVMERESFENEEIAAQLNRDFVAIKVDREERPDLDDVYMAA
                     VQAMTGGGGWPMSVFLTPELEPFFGGTYFPPEDRQGMPGFPRVLTAVADAYRNRRQDV
                     AAQARQLAAHLREQLDVRAGQVDPEQRQLDAAAARLAAAFDDVHGGFGGAPKFPAPMA
                     IEFLLRRWGRSRDPHLLHMVTFTLDRMAAGGIRDQLGGGFARYSTDARWLVPHFEKML
                     YDNALLAHAYLQAYRATGDERHAAVARATLDFMLRELVTEVGGLASALDADTDGEEGR
                     FYVWEHGEFTTVLREAGLDDGDVGALAEHWGVSAEGNWEGSNVLHLTGAEAASALVER
                     GRAALLAARERRTRPGRDDKQLASWNGMALRSLATGALVLGDQGYAGATQRLVTFIRE
                     RLLREGDRLWRTARNGNAHTPGFAEDYACVADGLLAAHAALGRSEDLVLAAALMDRLV
                     ADFWDESSGTLFDTGPEHERTLARPRSMVDGATPSANAVAADVLLRLALLTGEADHDR
                     RARSILRAVAPALDRQPAAFGRMLAAADRALAPAVDAVIAGSPSDRAALELRRAAARP
                     FAPDLVIAALEPDGQLSSLPLFEGKSARDGKPTAYVCRGYTCDEPTSDPGRVADQVAA
                     LSRPAGVSRAGS"
     gene            complement(9266..9679)
                     /locus_tag="VHK28_00035"
     CDS             complement(9266..9679)
                     /locus_tag="VHK28_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX2140650.1"
                     /translation="MHTRELSIETGSRRGVFDLTDACARFLSEVSDGGDGLLNVFVPH
                     ATAGVALVELGSGTDGDLMEALDRLLPRDDRWSHAHGSRGHGADHVLPAFLSPSVVLP
                     VVGGSMALGTWQSVALVDPNADNPRRKVRLSFLPG"
     gene            9734..10738
                     /locus_tag="VHK28_00040"
     CDS             9734..10738
                     /locus_tag="VHK28_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2140651.1"
                     /translation="MTHPRPPSFAVRVALLLTALILALAACSSAPTATPQPTASPSPT
                     QEPTPSPSPTPDPTPTPEPTPEPTPTRPDLDEALLHSRFTVLVVGEDSNSVRERMPFP
                     TQNTDAVMVVSVDATQSTVSMIGLPRDTVDVPMADGRIFRGKINSLAFGLGLPALQGA
                     MSTLLGVPIDGYVKIDMDNFVQLVDAVGGIPIEVPVGVYDPKLPIAIDPGSYLMSGKT
                     ALSYVRSRRDGDYARAGRQQDVLWALVQRYADPPPGTRLDAVLAMMDSLETNLDFSKL
                     ATFVTMARRAQDAEVSQIVLGPPRFALFEGIEPGTGRGWVMIPNISEIRSYARAVMSD
                     "
     gene            complement(10747..11559)
                     /gene="ccsA"
                     /locus_tag="VHK28_00045"
     CDS             complement(10747..11559)
                     /gene="ccsA"
                     /locus_tag="VHK28_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2140652.1"
                     /translation="MTVYPALLFAALGALLGSAMLSAAGIAMRRRGLLAAGHGGLLLA
                     GAFLLAALIMRGVVAGHAPWSNLHETAVTFAFGVVAVQGALERRLPVRALGAGAALIA
                     AGLLWLALAQPDEVRALVPALQAPLLLTVHVGSAMLAYAMAAVAAAAALAEIVQRRAG
                     DRIEALPPAAVARTAAHRAVLLGFPVLTAAILLGAVWANLAWRSYWSNDPKELAAAAT
                     WLVYGGYLHAAGRNDRWGRLAPWLLLLGFAAVLFTYLGAGLVFVGEHSYAGA"
     gene            complement(11617..12018)
                     /locus_tag="VHK28_00050"
     CDS             complement(11617..12018)
                     /locus_tag="VHK28_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2140653.1"
                     /translation="MDAIRDAIEWIADDSPGFVAGALPYLLLGLAVLYVLWVIVGYLR
                     VSQVGLETETAAQPVTRLPRAPDGVIEAPRGVPYCPVDGLQYPAGARFCTQCESDLAL
                     SCANCGATIRAADESCYRCGTRATTAVPELH"
     gene            12119..13192
                     /locus_tag="VHK28_00055"
     CDS             12119..13192
                     /locus_tag="VHK28_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2140654.1"
                     /translation="MPRPKLDPRTPLTELHVHLGAAVTPAIMWGIAHAQGIRLPTKDY
                     WAFRDLITVGRRRRGFQAYLELFHWTELIQSSPTAVERSVYEVIGGAYRKNNITEIEL
                     RFNPMKRNRGGEQDLDHIIAAALRGMDRAVLEYPQVRAGLIFCLDRAFSYELNEIIAA
                     KAIAWRDRGVVAVDIAGPVEPRFRFSDYRQLFAQVRRAGLGVTVHAGETGGADEVREA
                     IEALEPTRIGHGISSVGDRSVMAMLRERGIVLEVCPSSNLSTRVVSGLPEMRHILRTL
                     MDERVPFALSTDGPEMLRSYLRDEVALVLRHGMLSLDEVEQALETARRSTFVARDAQP
                     GRPSDGQFREPGPRAAIALEIEV"
     gene            13211..13870
                     /locus_tag="VHK28_00060"
     CDS             13211..13870
                     /locus_tag="VHK28_00060"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2140655.1"
                     /translation="MSSIWRPIRERSPARSLMAIVAHPGDEAFGFGGAIAQAAASGAY
                     VVVVCATRGRFDPRLLEKPPAPGGRNRHYSRPIVWRNLDTVREDEMRRSVALLGVRVL
                     RVLDYAETTLPDANFEELVGRLVEPIRMHRPEVILSFGPDGVTGDPDHVVLGRAADAA
                     FDLAGQPLAYEDDLEEDQVAWRAAKLYHLVVPAGQLAQAGEGYGTPAPATLSLELGEL
                     A"
     gene            13898..14059
                     /locus_tag="VHK28_00065"
     CDS             13898..14059
                     /locus_tag="VHK28_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140656.1"
                     /translation="MSQTGSAGPFHDWTPEMRNAYLGTEHYRLARSNLPVPAGSGDAT
                     ESSVWDGLH"
     gene            14177..15259
                     /locus_tag="VHK28_00070"
     CDS             14177..15259
                     /locus_tag="VHK28_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX2140657.1"
                     /translation="MAEQLTNENIMAVLGQVKDPEIGRDLVSLGMVKNIEINGSAIDL
                     TVELTTPACPLKAKIESDIVDGLESLGATAVRVDWASNVKRSFGGPAADLIPGVRNTI
                     AVASGKGGVGKTTVAVNLAVALARDGAKVGLLDADITGPNVPLMMGTVGSHVTGAGGR
                     VNPVTSHGVKMMSIQYFLTGDAPVIWRGPLIHSAIQQFLRDVEWGELDYLVIDLPPGT
                     GDAALSISQLIPLSGAVVVTTPQEVSLMDARKAIAMFNKVNVRTIGVVENMSAFICDC
                     GREHDIFGSGGGETIARELGLVVLGRIPLEPGVRTGGDAGVPITSTQHPSAETSAAAA
                     AMRELARSVAGRISVLAAPMAEVATA"
     gene            15335..>15758
                     /locus_tag="VHK28_00075"
     CDS             15335..>15758
                     /locus_tag="VHK28_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2140658.1"
                     /translation="MRIALLSDLHANLPALRAVLDDVASIGCDAVWCAGDVVGRGPHP
                     NEVVNELRRLEVPTVQGNWDEAASMGREVSGSIFATPEDEAQGHANLAWTGSRLTDEN
                     ITWLRQLPDKLRMEVHGRTALLFHGTPLKQNEYMWEDRP"
BASE COUNT         2332 a         5567 c         5575 g         2284 t
ORIGIN      
        1 ccggtgctac aacaaggcgc ccttcgcgac cgaagcccgt tcgcgatggg tttccttcct
       61 ggaatttgat gtccgcaacg cggttgcggg gagacggacc caccatgaca ctgggtgaga
      121 cggagacgac catccggcag cgccggcagt ttgccgagca ggccatttcg caggcgacgc
      181 gcggccagtg ggaggaggcg gtggagagca accgccagct cctggagctg ggcccggaca
      241 cggacgccta caaccggttg ggcaaggctc tcgccgagct cggccgccac gccgaagcct
      301 tggaggccta ccagcaggcg ctggcacggg atgccaccaa tcgcattgcc gagcgcaacg
      361 tggcgcggtt ggaggtgctg ctgggcgggg acaaggggga tcgcgacaac ggcaggacgg
      421 gcaaggcctc ggccgcgtcg tttatcgagg agatgggcaa gaccggtcac gcccggctga
      481 tcaacctggc cccggcgcgc cagctcgcgc cgctctcgcc gggcgacgcc gtggagctcg
      541 tcctggacgg agagatgctg gtcgccatgg ccggtgacgt cgcggtcggc caggtcgagc
      601 cccgggtcgg gtcgcgcctc gcgaagctga tgaagggcgg caaccgctac gaggcggcca
      661 tcaccgtcct ggatcgcgac gagtcccgga tcatcatccg cgaggtcttc gcccacccgg
      721 acaacttcgg caaggtttcg ttccccggca gcgcgaccgg ccgcgccggt gacgtaagac
      781 cgtacatgaa gggctccgcc ctccgctacg atgacgacga ggagagcgag gagcaggacg
      841 aggagcagga ggaagtcgag gagctcgaca ccaccctgcc tgagtactcg gctgagcccg
      901 acctcgaaga ggagctgctc gaagaaccgt aggaggcccg atgggcgagg ccggacggag
      961 gagactcgac cacgtcgtcc ttcctcgccg cctccccggg ctcgcgctcc tggagcggta
     1021 cttcccctcg ccggctgata accagctgga cgccgagctg tccgccttcg cccgcgccgg
     1081 cgacacggtg ctggatccct gggccgggac gggctggacc gcccgtcggg cgatcgcgca
     1141 tggcctgcgg gccgtctccg ccgatcccag ccccttcgct cagctggcgg cgcaggcttt
     1201 cctgctggcg cccgacccgt ccgccatcga ctccgccttc aaccagctcg ccagctcgcg
     1261 ccgggtggac gttccgctgc gccagcacat cgaggagctg tacgccagcc gctgcgcggt
     1321 ctgccgccac cccgtggtcg gtgaccagtt catctggcca cgtgacgcgg acgcgcccgg
     1381 ccgcaagatc tatcgctgct caaactgcga gatcagcgtg ggcggcgcgg aggagcgcgt
     1441 ggcgccagtg gacgaggtgg acctcgccaa gctcggcatc gagcggccga gcgacagccg
     1501 cgccgacatc gaatcggatc cgtcgataga cgctgaggac gacctgccgg cggcgccggt
     1561 cgggctgacg ggcgtggaaa caggaacgtc ggtagcggcc tacgatccag ccgaccactc
     1621 ggcgcgggat gcgctggggg agccgggtga gccaccgcca ccaccggcca tcacccccga
     1681 tgctcccacc gggccgggtt cagggcaggg acccggtccc agattcgcct ccaccgtgcg
     1741 ccccgatccg ctgccaccgc aggccgcaac cgaagtccgc aaggggccgc actaccagca
     1801 gctgcgcgac cgcttcccgg tgctggatgg gcagtcggcc ctcgtggacg agcttctcga
     1861 gctgtacacg ccgcgcaacc tgtacgcgct gcagacgatc gccaacaaga tcgacgccga
     1921 gttcagggag ccggcgcttt cggccgtctt tcgggtggcg ctggcggcct gcctgctgcc
     1981 cgccagccgc ctgaatggct atcccgggcg ggtggcatcc ttgcgggtca gcggtggcca
     2041 cgttcggcag ccggcgtcac gctaccagcg cgaggtcaat gtctggcggc tgttcgaggc
     2101 ggccgttcgc gatgtgcgga cggcgatcgc ggccctggga cgggacaggc gaccagcgcg
     2161 cttcgccgcg gggctggagg accttggcgg cgtgtcggcc gccaacgtgc tctggatccg
     2221 ctgccggccg gccgtgatcg gccagtacct tcccgaggag ggagtcgatc tggtgctgac
     2281 ctccatcgcc gcctcgggca gcgtcgacga gatcagcttc gaatacctcg ccaccgcctg
     2341 gctgatcggc cgtgaggcgg cggagaccct tcggctggag ccgctcttcg gttccgggcc
     2401 ggctcgatcc ggcgccgccg aggcgactgc cctgcggcat gccatcgcgt cggcagccgg
     2461 cgccctcaag ccggacggct ggttcgtggt catcctcgag ggcagtgacg tcgatcggct
     2521 gctgtcggtg gcggtggcgg gggcggccgg tggcctggac ctggtggacg tcatccaccg
     2581 tgagtcacgc cggtccgggg agggcgtgac cctgcacttt cacaagccct cggccgagga
     2641 ccggttgcgg gcggccgtgc agcccaagcc cctacggctg ggggctgagg acggccggct
     2701 cacctacccg gagctggcgg aggccatcga ccaggcgagc gtcggcctgc tgcggactcg
     2761 tggggagccc gccgggctga tccggatggt cgccgccgtg ctcctcgagc tgcagcggac
     2821 cgatctgctg cggcgggtgg cccaggcgcg tcccaacggc gaggcggcgg agcccagcga
     2881 cgccgagctg gaggggcgca tcgagcgcgg gggaggcctg ctgggcagcc tgctgcgcga
     2941 agagctggag cgcgacgatc atcccacgct ggttcgcctc ggcgagggcg gcgggtcgat
     3001 gtggtggctt cgagatccgg agatcgccga ttctccgctg gccgaccggg tggagtggtc
     3061 gaccttctcc gtgctctcga ccgccgggcg gttggatgcc gatggcttcc tcgagcgcat
     3121 ctacgccctc tttccgggcc tcgactcccc ggacgaggag ctggtgcggg cctgcctggc
     3181 cgcctacggc aggaccggtg acggcggcct gctacgcacc gatgacgacc tggggcgccg
     3241 cctggaggag cacagcgcgg tgatcgcggc gctggtggac tacgcgcatc ggtccggcct
     3301 gcgcacatgg gtcagccgcc gggagcagca ccgcgtggtc gagggccgga cgctggagga
     3361 gcggctgctg gaggacgagc ggcgcgccta cctgccgctc atcatccgcg cgccggccga
     3421 ggtcgtgggg gccgtggacg ccatctggta cgtccgcggt cgcctggccc tgctgttcga
     3481 ggtggagtgg acggccatgc tcggggagcc catcctgcgg cgcggccggc agatcccggt
     3541 cggcgatgag caggcccgct tcctggtgtt cccggccgag cgcacggagc tcgtgcggct
     3601 caagctggag cgctcgccgt ggctgcgggc ggaggtggag cgccagaact ggcatttcct
     3661 gaagtggcag cacctgcatg ccctggtcgc cggcgaggga gcgggcctgg agcgtctgga
     3721 gccgttcctg ggcctggacc cgctggccga gcgcggtggt cagcagctga ccatgttcgg
     3781 agagtgaccg agcgcttgct cacctagcgc acgatccttc ggcggctgct tgaccgtcag
     3841 gctatgctcg acccgggagt gccgtggttc ccccctgcgg cactcccatt ctccctgccc
     3901 gccgctagcc gccgtccgag cgcgtggccc gcttgccggt tgccggccgc ccaacggcac
     3961 gtcgcaccac ggtcatgacc tcgtcgacgt acggctggcc ctggccgctc tcgatggcgt
     4021 gggccaggca gccggcgatg tggtcctcca ggacgagcat cgacacctgg tccgcggcgg
     4081 ccgtcatggc gtccacctgc tgcaggatct ccaggcagta ggcctcgctc tccaccatgc
     4141 gctggattcc ccgcgcctga ccctcgattc gccgcagccg gttcagcagc tcctggcggt
     4201 ccttggtgta ggcgtggcgg aagtgcgcct cgcggctgga cctcatcgat ctggtgtctg
     4261 acatactggt gggtagtatg actgatgtcg aacgccacga actgatcacg ccgctggagc
     4321 gcgtgctcgt catcttcagc caccccgacg acgccgagtt cggcgccgcc ccgacgatcg
     4381 ccgccctggc gcggcttggc gcgcgggtgg actacctggt cacgaccgat gggggcaagg
     4441 gcaccgatga cccggccgtc actccccagc agctggcggc cacccgccgg gccgagcagc
     4501 gcgcggcggc tgacgtcctg ggcgtgagcg agatcgtgca cctcgactac cccgacggct
     4561 atctcacgcc cagcctcgac ctgcggcggg acatctcccg gcagatccgc cgcttccgac
     4621 ccgacctggt gatcgcccag aacccgcagc ggcgctccga cggcaacccg ttcatcgccc
     4681 accccgatca cctggcgacc ggggaggcga ccctggcggc ggtgtatccc gcggcgcgcg
     4741 accggctgaa cttcccggag ctgatcgacg aggggctcga gccgtggaag gtgcgccagg
     4801 tgctggtcac cggcgtggag cgtcccaacc tgtggatcga cgtgggcgag acgctggaga
     4861 ccggcatgcg ggcgctgcgc tgccatgtct cccaggtcga tgactgggag gccgtcgaga
     4921 cacggatcct ggagcggact gccgaggccg gcagggccgt ggccctggaa cgggcgcagg
     4981 ccttcctctc gatcctcctc caatgacccg acgcggccac tggggcggtg gcccggagcc
     5041 ggacctgacc gcccgcccgg ggagcggtcc ccgcacgctg cgcttgatcg ccgggttctt
     5101 ccgcccgtat cggttccggc tggggctgat cagcgtcctg atcctggtca ccgtcagcgt
     5161 gggggtggtc aaccccatcc tgctcaagct gatcatcgac aacctgctgg gtccgcagga
     5221 cctggggctg ctctacctcc aggtggcgct gatggtggtg ctgcccgtcg tgtcgtccgg
     5281 catcggcgtc tggcagtcct tcctgtccaa cgtggtcggg cagcgggtga tggacgacct
     5341 gcggctggcc ctttataccc acctgcaatg gatgccgctg cgcttcttca ccgagacgcg
     5401 caccggcgag atccagagcc gcatcggcaa cgacgtgaac ggtgtgcagt cggtggtgac
     5461 cgataccgcc gcctcgctcc tctccaactt cgcgaccgtg gccaccaccg tgatcgccat
     5521 gctgatcctg gactggcgcc tcacgatcct gtcgctcggc atgctgccgg tcttcgccta
     5581 catcaccttc cgggtcggca aggtgcgccg cgaggtcagc ggtatgaccc agcgctcgct
     5641 ggccgaggtg agcgccatca ccgaggagac gctgtcggtg tcgggcgtcc tgctgtcgaa
     5701 gaccttcggg cagcagcggg ccagcgtgga gcgcttccgt cgcgagtccc ggcgcctggg
     5761 cgacctgcag atccgccagc agatgatcgg gcgctggttc ttcgccatga tcggcacctt
     5821 cttcagcatc gcgccggcct tcgtctacct gctggccggg tcgctgatca tcggcggcga
     5881 cacgacggtc agcgtgggga ccatcgtggc cttcaccacc cttcagtccc ggctgttctt
     5941 cccgctcggc cagctcctca acgtgcaggt cgagatccag ggcgcgctgg ccctcttcga
     6001 ccgcatcttc gagtacctgg agatgccgca cgagatcacc gatgcgccag atgccgtcga
     6061 cctgaccacg gccaatactc gcggcgaggt gcgcttccgc ggcgtctcgt tccgctaccc
     6121 ggtctcgcct gggcaggtcc aggcgcaggc ggaggcggag ctggcagagg ccaccgggcg
     6181 cgccgtgcac ctccccatcc agcccttcgg cctgaccgat atcgacttca cggcccgccc
     6241 tggcgagctg gtggcgctgg tcggcccttc gggctccggg aagacgacga ccacctatct
     6301 ggtcccgcgc ctctacgacg tggacaccgg atcggtggag atcgacggcc acgacgtgcg
     6361 cggcatccgg ctggagtcgc tgggccgggt gatcggggtg gtcacccagg agacgtacct
     6421 gttccacgca tcggtcctgg acaacctgcg ctacgccaag ccggaggcga tcatggacga
     6481 ggtcgaggcc gccgcccgcg cggctgccat ccacgaccgg atcctcgagc tgccggaggg
     6541 atacgacacc atcgtagggg agcgcggcta caagctctct ggcggggaga agcagcgggt
     6601 cgcgatcgcc cgcgtgctgc tcaaggatcc gcgcatcctg atcctggacg aggcgacctc
     6661 cgccctggac accgtcagcg agcgcctgat ccaggccgcc ctcaatcggc tgatgcaggg
     6721 ccgcaccacc atcgccatcg cccaccgatt gtcgaccatc ctgcgcgccg accagatcct
     6781 ggtcttcgac cgcggccgga tcgtggagcg cggcacgcac gacgagctgc tgcgtcacga
     6841 gggcctgtac gcccggctct acgcggagca gttcgccgcg gagcagcccg ttccgcgctg
     6901 aggtggttcg cgccgcgccg caagccgtgt cggtgctgca ggatatcgac gagcgttcgt
     6961 gcgcgacccg ggatcgtgtg gaaatgtcgt accggtaggg cgtaacgggg aggatgtcgt
     7021 tcccggaggg cactccctgg cggtttcgtc ttcgcagcgc gtcgccggtg cgacatttcc
     7081 tgaggatgtg ccccttacga gcgcccgggc ttggcgatcg tggagttcct gctgtgtggg
     7141 tgcagcatgg gacgccgcgc acgggcgtat cggtccatct catgcgagac tggaaactcc
     7201 atgaccgatg cctcgccccc gcgccacacc aaccgcctcg ccgccgagac cagcccctac
     7261 ctgctgcagc acgcccacaa cccggtggac tggtacccgt ggggacagga cgccctggcc
     7321 cgcgccaggc tgctcgacag accgatcttc ctctcaatcg gctactcggc ctgccactgg
     7381 tgccacgtca tggagcgcga gtcgttcgag aacgaggaga tcgccgcgca gctgaaccgc
     7441 gacttcgtgg cgatcaaggt cgatcgcgag gagcggcccg acctggacga cgtgtacatg
     7501 gcagcggtgc aggccatgac cggcggcggc ggctggccga tgagcgtctt cctgacgccg
     7561 gagctcgaac ccttcttcgg cggcacctac ttcccgccgg aggaccggca gggtatgccg
     7621 ggctttccgc gggtgctcac cgccgtggcc gatgcctacc gcaaccggcg ccaggacgtg
     7681 gcggcgcagg cgcggcagct ggcggcccac ctgcgcgagc agctcgacgt gcgggccggg
     7741 caggtcgacc ccgagcagcg ccagctcgat gccgccgcgg cccgcctggc ggcggccttc
     7801 gacgatgtgc acggcgggtt cggcggcgcg cccaagttcc cggcgccgat ggccatcgag
     7861 ttcctgctca ggcggtgggg ccgcagccgg gacccacacc tgctgcacat ggtcaccttc
     7921 accctggacc gcatggccgc cggcggcatc cgggatcagc tcggcggggg gttcgcccgc
     7981 tacagcacgg acgcgcgctg gctggtgccg cacttcgaga agatgctgta cgacaacgcg
     8041 ctgcttgcgc acgcttacct gcaggcgtac cgcgccacgg gcgacgagcg gcacgcggcc
     8101 gtggcgcgcg ccacgctcga tttcatgctc cgcgagctgg tgaccgaggt cggcggcctg
     8161 gcgtccgccc tggatgcgga taccgacggc gaggaggggc gcttctacgt ctgggagcac
     8221 ggcgagttca cgaccgtcct tcgcgaggcc gggctggatg acggagatgt cggcgcactg
     8281 gccgagcact ggggagtcag cgccgagggt aactgggagg ggagcaacgt tctgcacctg
     8341 accggggcgg aggcggcgtc ggcgctcgtg gaacggggac gggcggcgct cctggccgcg
     8401 agggagcgca ggacccggcc cgggcgcgac gacaagcagc ttgcgtcctg gaatggcatg
     8461 gccctccggt ctttggccac cggcgccctg gtgctgggcg accagggcta cgccggcgcc
     8521 acgcagcgcc tggtcacctt catccgtgag cggctgctgc gcgagggcga tcggctatgg
     8581 cgcaccgcgc ggaatggcaa cgcccacacc cccggcttcg cggaggacta tgcctgcgtg
     8641 gcggacggcc tgctggccgc gcacgcggcc ctcggccgct ccgaggacct ggtgctggcc
     8701 gccgcgctga tggatcgcct ggtggccgac ttctgggacg agtcgtcggg aacgctgttc
     8761 gatacgggcc cggagcacga gcgcaccctg gctcgtcctc ggtcgatggt cgacggcgcg
     8821 acgccctccg ccaacgcggt ggccgccgac gtgctcctcc ggctggcgct gctcaccggg
     8881 gaggctgacc acgaccggcg ggcgcggagc atccttcgcg cagtcgcgcc ggccctcgac
     8941 cggcagcctg ccgcgttcgg ccggatgctg gcagcagcgg atcgtgccct cgcgccggcc
     9001 gtggacgccg tgatcgccgg gtcgccgtcc gatcgtgctg ccctcgagct gcgccgggcg
     9061 gcggcgcggc ccttcgcgcc tgacctggta atcgctgcgc tggagccgga cgggcagctc
     9121 tccagcctgc cgctcttcga agggaagtcc gcacgggacg gcaagccgac cgcctacgta
     9181 tgccgcggct acacgtgtga cgagccgacc agcgacccag gccgcgtcgc ggatcaggtg
     9241 gcggcgctga gccgtccggc gggcgtcagc cgggcaggaa gctgaggcga accttccgcc
     9301 gcgggttgtc ggcgttcggg tcgaccagcg ccaccgactg ccacgtccca agggccatcg
     9361 agccgcccac cacggggagc accaccgacg gggacaggaa tgcgggcagg acatggtcgg
     9421 cgccatggcc ccgcgagccg tgggcgtgtg accagcgatc gtcgcgcggc agcaggcgat
     9481 ccagcgcctc catgagatcg ccatccgtcc cgctgccgag ctcgaccagc gccacgccgg
     9541 ccgtggcgtg aggcacgaag acgttcagga ggccgtcgcc gccatcgctc acctcggaca
     9601 ggaaccgggc gcaggcatcg gtcaggtcga agacgccgcg acgtgagccg gtctcgatcg
     9661 agagctcgcg ggtgtgcatg ctccgagcgt accgagcagg cgggtatcgg tccagcgtat
     9721 gatcggcact gcgatgaccc acccgcgtcc cccgagcttt gccgtgcgcg tcgccctgct
     9781 gctgaccgca ctgatcctgg ccctggcggc ctgcagcagc gccccaacgg cgacgccgca
     9841 gccgacggcc agcccatccc cgacccagga gccgacgccc agcccgagcc cgacaccgga
     9901 ccccactccg accccggaac caacgccgga gccgacgccc acacgcccgg acctggacga
     9961 ggcgctcctg cacagccgct tcaccgtgct ggtggtgggc gaggactcca actcggtccg
    10021 cgagcggatg ccgttcccga cccagaacac cgatgcggtc atggtcgtca gcgtggatgc
    10081 cacgcagtcg acggtgagca tgatcggcct gccgcgcgac accgtcgacg tgcccatggc
    10141 cgatgggcgc atcttccgcg gcaagatcaa ctcgctggcg ttcgggctcg gcctgcccgc
    10201 gttgcagggc gcgatgtcga cgctgctggg cgttccgatc gacggctacg tcaagatcga
    10261 catggacaac ttcgtgcagc tggtggacgc ggtcggcggc atcccgatcg aggtcccggt
    10321 gggtgtgtac gacccgaagc tgccgatcgc catcgatccg ggctcatacc tgatgagcgg
    10381 caagacggcc ctctcctacg tgcgcagccg tcgcgacggc gactacgcgc gggccggtcg
    10441 gcagcaggac gtgctatggg ccctggtgca gcgctacgcg gacccgccgc ccggcacgcg
    10501 gctggatgcc gtcctggcca tgatggactc gctggagacg aacctcgact tctcgaagct
    10561 ggccaccttc gtgacaatgg ccaggcgggc gcaggacgcc gaggtaagcc agatcgtcct
    10621 cgggccgcca cgcttcgccc tcttcgaggg catcgagccg gggacaggac gtggctgggt
    10681 gatgatcccc aacatcagcg agatccgctc ctacgcccgc gcggtgatgt ccgactgagc
    10741 cccctttcag gcgccggcgt aggagtgctc cccgacgaag acgaggccgg cccccaggta
    10801 ggtgaacagg accgcggcga agcccagcag cagcagccag ggcgccaggc gtccccatcg
    10861 gtcgttccgc ccggcagcgt gcaggtagcc gccgtatacc agccaggtgg cagccgcggc
    10921 aagctccttg ggatcgttgc tccagtacga gcgccacgcc aggttggccc acaccgcacc
    10981 cagcaggatg gcggcagtca gcaccgggaa gccgagcagg accgcccgat gcgccgcggt
    11041 gcgggcgacc gccgccggcg gcagtgcctc gatccggtcg ccggcgcggc gctggacgat
    11101 ctcggccagc gcggccgctg ccgccaccgc ggccatggcg taggccagca tggcgctgcc
    11161 cacgtgcacg gtgagcagca gcggcgcctg cagtgcgggc accaaagccc gcacctcgtc
    11221 cggctgggcg agcgccagcc acagcaagcc ggcggcgatg agcgccgctc cggccccgag
    11281 cgcgcgaacc ggcagccgcc gctccagcgc gccctgcacc gccaccaccc cgaaggcgaa
    11341 ggtgaccgcc gtctcgtgaa ggttggacca cggcgcgtga ccggccacca cgccgcgcat
    11401 gatgagcgcg gccagcagga aggctccagc cagcagcaga ccgccgtggc cggccgccag
    11461 caggccgcga cgtcgcattg cgatcccagc ggcgctcagc atcgcagagc cgagcaacgc
    11521 gccgagggcc gcgaacagga gtgccgggta cacggtcatg gacggcccgc cagggatgcg
    11581 gagcgggccc gccggccggc ggtcaggcgg gagcgctcag tgcagttcgg gcacggcagt
    11641 ggtcgcccgc gtgccgcacc gatagcagga ctcatccgcg gcccgaatgg ttgcgccgca
    11701 gttcgcgcat gacagcgcca ggtcggactc gcactgcgtg cagaatcgtg cgccggcggg
    11761 gtactggagg ccgtcgaccg ggcagtaggg gacgccgcgg ggtgcctcga tgaccccatc
    11821 cggcgcgcgc ggcaggcggg tgaccggctg ggcggccgtc tccgtctcca ggccgacctg
    11881 cgagacgcgc aggtagccga cgatcaccca cagcacgtac agcaccgcca gccccaacag
    11941 caggtagggg agcgcgccgg cgacgaagcc ggggctgtcg tctgcgatcc attcgatcgc
    12001 atcgcggatg gcgtccatgg cgggccgatt ctagcatcgg ccgaccgcca ccagccgctg
    12061 cccgccccgc tgctacactc cgccaaactc gcagacccgg cgtatggcac gcacaccgat
    12121 gcccaggccc aagctcgacc cacgcacgcc gctgaccgag ctgcacgtgc acctcggcgc
    12181 ggccgtcacg ccggccatca tgtggggcat cgcccacgcc cagggcatcc ggctgccgac
    12241 caaggattac tgggcgttcc gcgacctgat cacggtcggg cgtcgacggc gcggcttcca
    12301 ggcctacctg gaactcttcc actggacgga gctgatccag tccagtccca ccgcggtcga
    12361 gcgcagcgtc tacgaggtga tcggcggcgc ctaccgcaag aacaacatca cggagatcga
    12421 gctgcggttc aacccgatga agcgcaaccg cggcggcgag caggacctgg accacatcat
    12481 cgccgccgcg ctgcgcggca tggaccgcgc cgtgctggag tatccgcagg tccgggccgg
    12541 gctcatcttc tgcctggacc gcgccttcag ctacgagctc aacgagatca tcgccgccaa
    12601 ggccatcgcc tggcgcgacc gcggcgtggt ggcggtcgac atcgcgggtc cggtggagcc
    12661 acgcttccgc ttctcggact accggcagct ctttgcgcag gtgcgccgcg cgggcctggg
    12721 cgtgacggtc cacgccgggg agaccggtgg ggccgacgag gtgcgcgaag ccatcgaggc
    12781 cctggagccg acgcgcattg gtcacggcat cagcagcgtc ggtgaccgtt ccgtgatggc
    12841 catgcttcgg gagcgcggca tcgtgctcga ggtctgcccg tcctcgaacc tgagcacccg
    12901 ggtcgtcagc ggcctgccgg agatgcggca catcctgcgc accctcatgg acgagcgggt
    12961 gccattcgcg ctctcgaccg atggaccgga gatgctgcgc tcctacctac gcgacgaggt
    13021 ggcactcgtg ctgcgccacg gcatgctctc cctcgacgag gtcgagcagg cgctggagac
    13081 ggcgcgcagg tcgaccttcg tggcccgcga tgcgcagccc ggccgcccgt ccgacggcca
    13141 gtttcgcgag cccggaccgc gtgccgcgat tgccctcgaa atcgaggttt aggctgatag
    13201 actcgcggcc gtgtcatcga tctggcgacc catccgcgag cggagtcccg ctcgcagcct
    13261 gatggccatc gtcgcccacc cgggcgacga ggcattcggc ttcggtggcg ccatcgccca
    13321 ggccgccgct tccggcgcct acgtggtcgt ggtatgcgcc acccgcggcc gcttcgaccc
    13381 tcgcctgctg gagaagcctc cggcccccgg cggacgcaac cggcactact cgcgacccat
    13441 cgtctggcgc aacctggaca ctgtccgcga ggacgagatg cgccgctccg tcgcactgct
    13501 gggggtccgg gtcctgcggg tcctggacta tgcggagacc acgctgccgg acgcgaactt
    13561 cgaggagctg gtcggccgcc tggtggagcc catccgcatg caccggccgg aggtgatcct
    13621 gagcttcggg ccggacggcg tgacgggtga cccggaccac gtcgtcctgg gccgcgcggc
    13681 ggatgccgcc ttcgacctgg cgggacagcc gctggcctac gaggacgacc tcgaggagga
    13741 ccaggtggcc tggcgcgcgg cgaagctgta ccacctggtt gtccccgcgg ggcagctggc
    13801 gcaggcgggg gaggggtacg gaacgccggc gccggcgacc ctgagcctgg aacttggcga
    13861 gctcgcctag ctcaaggagg cggccatcgc tcgccacgtg tcgcagaccg gatcggccgg
    13921 cccgttccac gactggaccc cagagatgcg caacgcgtac ctgggcaccg agcactaccg
    13981 gctcgcgcgc tcgaacctgc cggtacccgc cggcagcggc gacgccaccg agtcatcggt
    14041 ctgggacggg ctgcactgag cgatttctcc cgcggatggg cccaatctca acatcggtag
    14101 catcgctcca tcccgagtct ctgctaccct cgatgcagcc cctcccatcg gtagagaaag
    14161 gaagccgacc tccggcatgg ccgagcagct gacgaacgag aacatcatgg ccgtcctggg
    14221 ccaggtcaag gaccccgaga tcgggcgcga cctggtgagc ctgggcatgg tcaagaacat
    14281 cgagatcaac gggtcggcga tcgacctcac cgtggagctc accacgcccg cctgtccgct
    14341 caaggcgaag atcgagtcgg acatcgtgga cggcctcgag tcgctgggcg ccacggcggt
    14401 gcgcgtcgat tgggcgagca acgtgaagcg ctccttcggt gggccggcgg ctgaccttat
    14461 cccgggcgtg cggaacacga tcgcggtcgc ctccggcaag ggcggcgtgg gcaagaccac
    14521 ggtcgccgtc aacctggcgg tggcgctggc gcgcgacggc gcgaaggtcg gcctgctgga
    14581 cgccgatatc accggcccca acgtgccgct gatgatgggc acggtgggct cgcacgtgac
    14641 cggcgcgggt gggcgggtga acccggtcac cagccacggc gtgaagatga tgagcatcca
    14701 gtacttcctc accggcgacg cgccggtcat ctggcgcggc ccgctcatcc actcggccat
    14761 ccagcagttc ctgcgcgacg tcgagtgggg agagttggac tacctggtca tcgacctgcc
    14821 gccggggacc ggcgacgcgg cactgtccat cagccagctg atcccgctct cgggcgcggt
    14881 ggtggtcacc acgcctcagg aggtgagcct gatggacgcg cgcaaggcga tcgccatgtt
    14941 caacaaggtg aacgtgcgca ccatcggcgt ggtggagaac atgagcgcct tcatctgcga
    15001 ctgcggccgg gagcacgaca tcttcggctc cggcggcggc gagacgatcg cgcgcgagct
    15061 ggggctggta gtgctgggac gcatcccgct ggagccgggc gtgcggaccg gcggggatgc
    15121 cggcgtgccg atcacctcga cccagcatcc ttccgccgag acatccgcgg ccgcggccgc
    15181 catgcgcgag ctggcccgca gcgtagcggg ccgcatcagc gtgctggcgg cacccatggc
    15241 cgaggtggcg accgcctagc aacgctcccg agcggcccga atgtactgcg cgagtgggat
    15301 ggtcgggctg tacatcgctt cctagaatcc gggcatgcgg atcgccctgc tgtcggacct
    15361 gcacgccaac ctgccggcgc ttcgggccgt gctcgacgac gtggcctcca tcggctgcga
    15421 tgccgtctgg tgcgcggggg acgtggtggg tcgaggcccg caccccaacg aggtcgtcaa
    15481 cgagctgcgc cggctggagg tgcccaccgt ccagggcaac tgggacgagg cggccagcat
    15541 gggtcgcgag gtgagcggca gcatcttcgc cacccccgag gacgaggccc aggggcacgc
    15601 caacctggcc tggacgggct cccgcctgac cgatgagaac atcacctggc tccggcagct
    15661 gccggacaag ctgcgcatgg aggtccacgg gcgcactgcg ctcctcttcc atggcacgcc
    15721 gctcaagcag aacgagtaca tgtgggagga ccgcccgt
//
LOCUS       DATACA010000002         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_723114, whole genome shotgun sequence.
ACCESSION   DATACA010000002 DATACA010000000
VERSION     DATACA010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723114"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..934)
                     /locus_tag="VHK28_00080"
     CDS             complement(164..934)
                     /locus_tag="VHK28_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2140659.1"
                     /translation="MRDIDLGRIVRALRRRRGWRQIDCSTRAGVHRSTWSSIERGHFD
                     RLSLLILRRCLAVLDVKLDLLPSWRGAQLSRLMDEGHAALQAEWKRRLEAMGWLVRVE
                     VSFNHYGDRGRIDLVAWHPRLQILVIAEVKTEIADAQGLLGALDVKVRVAPNVAKALG
                     LGRPRLILPLLIVADGSTNRDRIARLQPLFARFTIRGRQAAAWLRNPRESVVGLLVFT
                     DLRNANGGSVTQVTPHRIRPTRRDLSVEKGSEGAAAAS"
     gene            1119..1604
                     /locus_tag="VHK28_00085"
     CDS             1119..1604
                     /locus_tag="VHK28_00085"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2140660.1"
                     /translation="MHEWIVYAHVLGAFVFILAHGVSMFTSFRLRGTRDPARVAELLE
                     LSGRSIGLLYIGLLVLLAAGVAAGFSGGFWGRGWIWAAIGLLVVILAVMYSVAAPFYG
                     RMRAAAGIPGYVEKANDFKPPATPEDLQKLARSSRPIWLAAVGGIGLAAIIYLMVVKP
                     F"
     gene            1604..2056
                     /locus_tag="VHK28_00090"
     CDS             1604..2056
                     /locus_tag="VHK28_00090"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX2140661.1"
                     /translation="MEPQRFRSVIEAARGGGAVALIPPDATQALGGLKQMRVTGTLNG
                     TTFRSSTMPYRGAFYLGVHKATREAAGVQIGDEVELTVTRDDSPRVLELAPELEEALS
                     AEAGLRAKFDALSFSRRRELADPVREAKRPETRRTRVEKALARLRELP"
     gene            complement(2039..2869)
                     /locus_tag="VHK28_00095"
     CDS             complement(2039..2869)
                     /locus_tag="VHK28_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX2140662.1"
                     /translation="MPEGDTIFRTADVLRRALRGSTVVRAVAQPRPGLARVPDLSVLV
                     GTRVEEVESRGKHLLIGFSGGWWLRSHMRMRGSWHRYRVGEEWRLPRSRATCVLETST
                     AVAVCFDAPELELLTDVSLARHARLDALGPDLLARSFDLEDAVGRLSADPRRPLGEAL
                     LDQRAVAGMGNVYKSEVCFLERLDPWMPVGLVPAPTLRAALTTARRLLAANAGGGRRV
                     TTGVGSPGRSLWVYSRAGRPCRRCGTLIQSRRQGEDGRLTYWCPRCQGGGSGDSGELA
                     "
     gene            2962..3593
                     /locus_tag="VHK28_00100"
                     /pseudo
     CDS             2962..3593
                     /locus_tag="VHK28_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119790.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
BASE COUNT          591 a         1241 c         1227 g          534 t
ORIGIN      
        1 cggctacgag cagatcgagg ccaagctcgt ggccctgggc gcccagatcc accgcatcga
       61 cgcctgaccc gcgtgcggtc gccgggctgt cggtttcagg cgacatcccg tggctcggct
      121 gcgtgtgact gccgatatgc gaattcccga agcgttacgg cggtcacgag gccgcagcag
      181 ccccctctga gcccttctcc acgctcagat cccgacgcgt ggggcggatt cggtgcggtg
      241 tgacctgcgt aacgcttccc ccattcgcat ttcgcaagtc ggtgaaaacc aggagtccca
      301 ctacggactc gcgcgggtta cgcagccatg cagccgcctg gcgaccgcga atggtgaacc
      361 gggcgaagag cggctgcaga cgagcgattc gatcgcggtt cgtggagccg tccgcaacga
      421 tcagcagcgg aaggatcagg cgcggccgtc ccaatcccag cgccttcgcc acgttcggcg
      481 cgactcgcac cttcacgtcc agcgcgccca gcagcccttg ggcgtccgca atctcggtct
      541 tcacctccgc gatgacgagg atctgtagcc gcgggtgcca ggcaaccagg tcgatgcgcc
      601 cgcggtcgcc atagtggttg aagctcacct ccacccgcac tagccacccc attgcttcga
      661 gacgccgctt ccattcggcc tgtagggcgg cgtgaccctc gtccatcagc cggctcagct
      721 gggcaccgcg ccagctgggc agcagatcca gcttcacgtc gaggaccgcc aggcagcgtc
      781 gcaggattag gagcgagagc cgatcgaagt gcccgcgctc gatcgaggac cacgtggacc
      841 gatgcacgcc agcccgcgtg ctgcagtcga tctgccgcca gccgcgtcgt cgtcgaagag
      901 cccgcacgat ccgaccgaga tcgatatctc gcatcttgac agtctgtcgc tggacccgtt
      961 accgggtgct tactggacgc ttacccaagg cgtgtcgggc ccctgccggt acgcgatccc
     1021 cgaagcgata gggcaccatt cgcctctcgg aagtcagcca gccagaccgg agcccaatag
     1081 agttgcgggc gggccggctg gcgccgtagt tttggcggat gcacgagtgg atcgtctatg
     1141 cccacgtcct cggggccttc gtgttcatcc tggcccacgg cgtgtcgatg ttcacctcct
     1201 tccgcctgcg tggcacgcgc gatccggcgc gggtggcgga gctgctggag ctgtcgggca
     1261 ggagcatcgg cctgctgtac atcgggctcc tggtcctgct ggcagcgggt gtcgcagctg
     1321 gattcagcgg tggattctgg gggcgcggct ggatctgggc tgccatcggc ctgctggtgg
     1381 tgatcctggc ggtgatgtac tcggtggcgg caccgttcta cgggcggatg cgagcggcgg
     1441 ccggcattcc cggatacgtg gaaaaggcga acgacttcaa gccgccggcg accccagagg
     1501 acctgcaaaa gctggcgcgc tccagccggc cgatatggct ggcggccgtc ggagggatcg
     1561 gtctggcggc catcatctac ctcatggtcg tgaagccgtt ttgatggagc cgcagcgctt
     1621 ccgaagcgtg atcgaggcgg cgcgcggtgg cggggccgtc gccctcatcc cgcccgatgc
     1681 cacgcaggca ctcggtgggc tcaagcagat gcgggttacc ggcaccctca acgggacgac
     1741 gttccgcagc agcaccatgc cctatcgcgg cgcgttctac ctcggcgtgc acaaggcgac
     1801 tcgcgaggcg gccggcgtgc aaattggcga cgaggtcgag ctcaccgtta cccgcgacga
     1861 ctcgccgcgg gtccttgagc tggcacccga gctggaggaa gccctcagcg cggaagccgg
     1921 cctccgagcg aagttcgacg ccctctcctt cagccgccgt cgggagctgg cggacccggt
     1981 gcgcgaggcg aagcgcccgg aaacgcgaag gacgcgcgtg gagaaggcgc tcgcacgcct
     2041 acgcgagctc ccctgaatct cctgacccgc cgccctggca tcgcgggcac cagtaggtga
     2101 ggcggccgtc ctctccctgg cggcgcgact ggatgagcgt gccgcatcgg cggcagggcc
     2161 ggccggcgcg gctgtagacc cacagcgagc gaccgggcga tccgaccccg gtcgtgactc
     2221 ggcggccacc acccgcgttt gccgccagca gccggcgcgc cgtggtcagc gcggcgcgca
     2281 aggtgggcgc tggaaccagg cccaccggca tccacgggtc cagccgctcc aggaagcaga
     2341 cctccgactt gtagacgttg cccatcccgg ccaccgcccg ctgatccagc aacgcctccc
     2401 cgagcggccg ccgcggatcg gctgacaacc gccccacggc atcctccagg tcgaaggagc
     2461 gggccagcag gtccgggcca agggcgtcga gccgggcgtg gcgcgccagg gagacatcgg
     2521 tcaggagctc gagctccggc gcgtcgaagc agacggcaac cgccgtggaa gtttccagca
     2581 cgcaggtcgc gcgtgaacgg ggcagcctcc actcctctcc cacccgatac cgatgccacg
     2641 agccgcgcat ccgcatgtgc gagcgcagcc accaaccgcc ggagaagccg atcagcaggt
     2701 gcttgcctcg cgattccacc tcctccacgc gtgtgccgac caggaccgac aggtcgggaa
     2761 cgcgggccag tcccggccgt ggctgggcga cggcgcgcac cacggtcgag ccgcgcaggg
     2821 cgcggcgaag gacatcggcc gttcggaaga tcgtgtcgcc ttcgggcatg ggtttgaacg
     2881 gatcgtaccg gccgggtcga ggaggcttat cggtatactg cgggcgcccc agccagcccc
     2941 gcacgagagc agcacgcgcg catgaagatc ggcatcgacc tcggcaccgc caacatcctg
     3001 gtctacgtca agggcaaggg cgtcgtgctc aacgagccga gcgtcgtggc catcagcgac
     3061 gacgacaacc gcctggtggc ggtgggcgag gaggcgcgcg agatgatcgg tcgcaccccc
     3121 ggcaacgtac gcgccatccg gcccatgcgc gacggggtga tcgccgacta cctcatcacc
     3181 gaggccatgc tgcgctacgt catcaacaag gtggcacggg tccggctctt caagccggac
     3241 gtgatgatca gcgtgccgtc cggcgtgacc agcgtggaga agcgcgccgt gcgtgacgcc
     3301 gccctcaagg ccggcgccaa ggaggcgttc ctgatcgagg agccgctggc ggcggcgatc
     3361 ggcgccaacg tgcccatctc cggccgtcag gcaacctgat catcgacatc ggcggtggca
     3421 ccagcgagat cgcggtcatc gcgctgggcg ggatcgttgt cagccagagc gtgcgggtgg
     3481 gcggcaaccg cttcgatgag gcgatcacca actacatccg ccgcaagtac aacctgatga
     3541 tcggcgaccg gacggcggag gaggtcaaga tcgccatcgg ttccgcgctg ccc
//
LOCUS       DATACA010000003         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_160891, whole genome shotgun sequence.
ACCESSION   DATACA010000003 DATACA010000000
VERSION     DATACA010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160891"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..999)
                     /locus_tag="VHK28_00105"
     CDS             complement(226..999)
                     /locus_tag="VHK28_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2140663.1"
                     /translation="MDTEIWALALAGLAGLACGFLNTAASSGSAVSLPILMAIGLDPV
                     TANATNRLPVLIGAMSGTGSFHRKRAIPWRNALVVAVPVTAGAGVGAVLAEVIPSRDL
                     ALVITAAVLVALLLLFTKLKSAIANAEVRDTRLGAREIAIFTGIGVWLGFIVLDGATY
                     LLLALTLAVGLPLVQANAVKTFALVPTSLLALVIFATKGHVDWEVGAVMAVTAVAGGI
                     LGAKLTLSPAARTWVFRLLVLVILGELVHLTMHYVFEAV"
     gene            complement(1133..2059)
                     /locus_tag="VHK28_00110"
     CDS             complement(1133..2059)
                     /locus_tag="VHK28_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2140664.1"
                     /translation="MTVPMAGDFRRRRITVDGVGINTLTAGSGPPVLLLHGYPQTHLV
                     WHHVAPELAAEYTVVLTDLRGYGDSDKPESSPDHATYAKRAMARDQHTVMSTLGFERY
                     GVVGHDRGARVGHRMGLDYPEAVVAFAALDVVPTLHAFTHVDVEFARGYYHWFFLTAG
                     NGIPERLIGADPGFWVRARMGSRHHGGVPIDEAALSEYVRCFSDPEAIRASCEDYRAA
                     ATIDLEHDRADADADRSLRCPTLLLWGAHSFVGRHYDVPAAWRRYAPRVHGVALPCDH
                     YVPEEAPAETLEHLVPFLHQHVGGVDGQHGRH"
BASE COUNT          328 a          727 c          797 g          302 t
ORIGIN      
        1 tcgaagtccc ggacctggta gtccgggact tcggctttcc aggcctctga cctgcctctt
       61 cacggtcggg ccggccatct gctcgccggt gtcgcgccct cgtcgaccag gaaccggtaa
      121 ggctggggct tctcgcatgc ctgtcccgcc cagctccttg cactggtagc cctggatgct
      181 gatcgcgtgt gagtgccgaa tcggtgcagc caggtgggtg acgagtcaga cggcctcgaa
      241 cacgtagtgc atcgtcaggt gcaccagctc gccgaggatg accaggacca gcagccggaa
      301 gacccaggtc cgcgccgccg gcgacagcgt gagcttggcc ccgagtatgc cgccggcgac
      361 ggcggtcacg gccatcacgg cgccgacctc ccagtcgacg tggcccttcg tcgcgaagat
      421 caccagggcc agcaggctgg tcgggacgag ggcgaacgtc ttcacggcat tggcctggac
      481 cagcggcagg ccgactgcga gcgtgagcgc gaggagcagg taggtcgccc cgtcgagcac
      541 gatgaagccg agccagaccc cgatgcccgt gaagatcgcg atctcgcgtg cgcccagccg
      601 ggtgtcgcgc acctcggcgt tcgcgatcgc tgacttgagc ttggtgaaga gcaggagcag
      661 cgcgaccagc acggcggccg tgatcaccag ggccaggtca cgggacggga tcacctcggc
      721 gaggaccgcg cccacgcccg cgccggcggt caccggaacg gccacgacca gtgcgttgcg
      781 ccaggggatc gctcgcttgc ggtggaagga gccggtgccc gacatcgcgc cgatcagcac
      841 cggcaggcgg ttggtggcgt tggcggtcac ggggtccagg ccgatcgcca tcaggatcgg
      901 aagcgagacc gccgaccccg aggacgcggc ggtgttcaag aagccgcacg ccaagccggc
      961 caggccggcc aacgccagcg cccagatctc ggtgtccacc gcgtcctccg gtcggcaaaa
     1021 cggccgtgac tgactcggcc attccacagc ggggaggtcg cgggccgccc gctgacacgg
     1081 cctgatccat cgcgaacctg cgcctcgccg tggtcgcggc gtgtgcgtga cgctagtggc
     1141 ggccatgctg gccgtccacc ccgccgacgt gctggtggag gaacgggacc aggtgctcca
     1201 aggtctcggc cggcgcctcc tcgggcacgt agtggtcaca gggcagcgcg acgccgtgga
     1261 cgcgtggggc gtaccggcgc caggcggcgg gcacgtcgta gtggcgcccg acgaagctgt
     1321 gcgcgcccca cagcaacagc gtggggcagc ggaggctgcg gtcggcgtcg gcgtcggcgc
     1381 ggtcgtgctc gaggtcgatg gtcgccgcgg cccggtagtc ctcacacgag gcgcggatcg
     1441 cctcggggtc ggagaagcag cggacgtact cgctgagggc tgcttcgtcg atgggcacgc
     1501 ccccgtggtg gcggctgccc atgcgcgcgc gaacccagaa gccagggtcg gcgccgatga
     1561 ggcgctcggg gatcccgttc cccgcggtca ggaagaacca gtggtagtag ccgcgcgcga
     1621 actcgacgtc cacgtgggtg aacgcgtgca gggtcggcac gacgtccagg gcggcgaagg
     1681 cgaccacggc ctcggggtag tccaggccca tgcggtgtcc cactcgtgcg cctcggtcgt
     1741 gaccgacgac gccgtagcgc tcgaaaccca gcgtggacat caccgtgtgc tggtcacggg
     1801 ccatggcgcg cttggcgtag gtggcgtggt caggtgagga ctccggcttg tcgctgtcgc
     1861 cgtagccgcg gaggtcggtc agcaccacgg tgtactccgc ggcgagctcc ggggccacgt
     1921 ggtgccagac cagatgcgtc tgcggatacc cgtgcagcaa cagcaccgga gggcccgagc
     1981 cggcggtcag cgtgttgatg ccgacgccgt cgacggtgat gcggcgccgt cggaagtcgc
     2041 cggccatcgg aaccgtcatg gcatcgtcct cccgccggga cggcagtgca gtggcgctcg
     2101 ccgaggaccc ggcgagcgct tgtccgcaaa cgcaggtgag cgggccgtct ccgt
//
LOCUS       DATACA010000004         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_964261, whole genome shotgun sequence.
ACCESSION   DATACA010000004 DATACA010000000
VERSION     DATACA010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964261"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..407
                     /locus_tag="VHK28_00115"
                     /pseudo
     CDS             6..407
                     /locus_tag="VHK28_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            1542..1928
                     /locus_tag="VHK28_00120"
     CDS             1542..1928
                     /locus_tag="VHK28_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX2140665.1"
                     /translation="MLSRFVASYPSLFGAKASERAIVSGVPASSGIATGTVRVVRGPH
                     EFDQLQAGEVLVAPFTAPAWTPLFTRAAAVVTDVGSAAAHASIVAREYGIPAVVGCGD
                     ATARLRPGMRVTVDGSTGNVEPAGDA"
     gene            complement(1931..2374)
                     /locus_tag="VHK28_00125"
     CDS             complement(1931..2374)
                     /locus_tag="VHK28_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2140666.1"
                     /translation="MRRIEASATIPVPPERLFAFVADPANLPQWQTGIVSAERTTPDP
                     VGVGSEARVVRDVLGRQVAANLTITDYEPGRLLGLSTAVSGIGARAALELAPDGPDAT
                     RLTFSMEIRAESLFMAPVEGMVAQGAQQDVASSLERLREHFTADG"
BASE COUNT          337 a          807 c          887 g          377 t
ORIGIN      
        1 ccggcatgtt cgaaacggtg ctggatgtgg acgccggcga ccttcaggca gcggcgggtc
       61 gggtgctggc cagcgccgat gcagcccgcc cagccgcgta tggaccagtc gacggcgtgg
      121 gcatgggagt gatcgtccag cgcatgctgt ctccgctggc agccggcgtg gtcctcaccg
      181 ccgaccccat cacgggtgac cgagggactg ttgtcgtcaa tgccgtgcgc ggcttgggcg
      241 atcggctggt ctctggcgcg gccgtcggag acgagtgggt ggtccgcggc gaggacgctg
      301 acgcccgtcg cctgctggaa cgtgcgatcg accgcgacca ggcgctcgag atcgctgccg
      361 aggcccggcg catcaccgag gtccagggta ggccgcagga catcgaatgg gccatcgatg
      421 cggatggatc cctctggatc ctgcaggcca gacccatgac ggcgcttccg cccgatgtgt
      481 cgtgggtgcc tgacgcccca ggggcctatc accgatccct gcggttcggc gagtggatcg
      541 gtgagccggt gacacccctg ttcgagtcgt ggctgctgtc cggtctggag gttggactgc
      601 acgcgtggat caaacaggag ctcggccagc gcgcgccact gccccaccac gtcattgtca
      661 acggctggta tttctactcg ctcaactgga tcgctccgcg gaacatgctt cgcagcctgc
      721 caagcatcct ctggcatgcc gcccgctcgc ctcgcgcagt cgccggcgtg atcccgcgga
      781 cggttcggca cagcttcccg atcatggaag ggaggtggcg ggctgatctg cagccgcggt
      841 accgcgccgc ggtggatgat gccgaggagc gcgtcgacga gcagccggtc tccgagctac
      901 cggcactgat cgacgagatg accgggctgg ccggggagta cttcggctcg ctggcggcgc
      961 tgggcggcgc cgcctacaag atggagatca acctggcgaa gttctatcgc aagcacctgg
     1021 agccgacgct gggtggaagc cacctgccgc tcctggccgg catcgaggcg ccgtcggaac
     1081 ccgagccgca cgccgtcgtg tcgctcgact ggtggttcgc accggggcgg caggctgggt
     1141 cgccaaccgg agcggcgaag cacgcccgcg tggtcgcagc caggcaggca gccgaggatg
     1201 cggcgctggc ggcccttacg gcgtcgcccc gccgcctgcg agagttccag gagatgctgg
     1261 cggatgggca gcacctcgtc tccatccgcg aggagcaggt gcgggagtgg acgcgaccct
     1321 ggccggtcat gcggcgggcg gtcatgcgca tcggcgagtc gctcgtcggg cgcggtcttc
     1381 tcgagaacgc cgaggatgtc ttcttcctct acagggatga ggtaatcggt ggactgcgtg
     1441 atggggaggt gcctgccgcg gcgagggtac tcgaccggcg ggcccagcga gaggagcagg
     1501 ccaggctggt gccgccgatg gccgtcggaa agtggaatcc gatgctttcc cgcttcgtcg
     1561 ccagctatcc gtcgctgttt ggggccaagg cgtccgagcg ggcgatcgtg tccggcgtgc
     1621 cggcatcgtc cggtatcgcc actggcaccg tacgggtggt ccgtggcccc cacgagttcg
     1681 accagctgca ggctggcgag gtgctggtgg cgccgttcac cgctccggcg tggacgcccc
     1741 tcttcacgcg cgccgctgcc gtggtgaccg atgtcgggag cgctgccgcc cacgcgtcca
     1801 tcgttgcccg tgagtacgga attccggcag tcgtgggctg cggcgacgcc acggcccggc
     1861 tccggccggg tatgcgcgtg acggtggacg gcagcaccgg aaacgtggag ccggcaggag
     1921 acgcctgaca ctacccgtcg gccgtgaagt gctcgcgcag gcgctcgagc gagctcgcca
     1981 cgtcctgctg tgccccctgg gcgaccatcc cctccaccgg cgccatgaac aggctctcgg
     2041 cgcggatctc catgctgaag gtcagccggg tcgcgtccgg gccatcgggc gccagttcta
     2101 gggccgccct ggcgccgatg ccgctgaccg cggtcgagag gcccagcagg cggccgggct
     2161 cgtagtcggt gatcgtcagg ttggccgcca cctgccggcc gaggacgtcg cgcaccacgc
     2221 gtgcctctga ccccacgccg accgggtccg gggtcgttcg ctcggcggag acgatgccgg
     2281 tctgccactg cggcaggttg gccgggtccg ccacgaaggc gaagagccgc tcgggcggga
     2341 cggggatggt ggccgatgcc tcgatgcgtc gcacgcggca accctacccg acccgaggcg
     2401 accagcgc
//
LOCUS       DATACA010000005         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1767582, whole genome shotgun sequence.
ACCESSION   DATACA010000005 DATACA010000000
VERSION     DATACA010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767582"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..984
                     /locus_tag="VHK28_00130"
     CDS             127..984
                     /locus_tag="VHK28_00130"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX2140667.1"
                     /translation="MRAARLAAPAKVNLCLAVIGRRPDGYHELVTDMVLLELADELLL
                     LPGCAGLRVSGATAAGVPVSPSQNLAWRGLLAAVGGAPAAACLELDKRIPAAAGLGGG
                     SSDAAAGWRLGRLLAGRDDPPTGSEVAALVAVGADVPFFAATVAAARVTGVGEDVAPI
                     AVPATAEVVLAEAPDGLSTAAVFAELRAGEWSTEPDPEQNDLLAAARRLRPEIDELFR
                     MVTAAGGAPRLSGSGPTVFSLTDDPERADAVVRRLLRAGVRASRTRLRREPASIELLD
                     QQDGQHEEV"
     gene            1005..1376
                     /locus_tag="VHK28_00135"
     CDS             1005..1376
                     /locus_tag="VHK28_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2140668.1"
                     /translation="MATNAAPAAIGPYSQGIRMGELVFTAGQIGLDPASGELAEGVTA
                     QAERALRNVAAILDAAGSSLERVVKTTIFLADMNDFNAVNEVYATHFATPYPARSTVA
                     VAGLPRGALIEIEAIALAGEG"
     gene            1462..>2708
                     /gene="glmU"
                     /locus_tag="VHK28_00140"
     CDS             1462..>2708
                     /gene="glmU"
                     /locus_tag="VHK28_00140"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX2140669.1"
                     /translation="MVATAAIVLAAGQGTRMRSRLPKVLHPLAGRPMLDHVLDALAAA
                     GVGHRVVVTGHGAQQVEATLDDRVPTVRQDPQLGTADAVRCGLSRVPPEARHVVVTMG
                     DVPLLPAELFQRLLLEQAEGEATIALLSARLPEPVGYGRVVRAQDGSVRAIVEERDAD
                     EATRSLDEVNVGTYCFDAAWLRANVGNVPASASGEYYLTDLVALATAGGRRVAVVSAP
                     RPELTTGINDRVALAEAERLLRAEIAERHMRNGVTIVDPANTFIDAGVEIGQDVRIEP
                     WTVLSGTTAIAQDAVIGPNATVRDSRIGPRTSVWASVIESSEVAEDVEIGPYSHLRPG
                     CRIGPRCRIGNFAELKQTRMGSGTQQHHFSYLGDAQVGQDVNIGAGAVTANYDGAQKH
                     PTMIGDGAFIGVDTMLRAPITIGP"
BASE COUNT          372 a          975 c         1004 g          357 t
ORIGIN      
        1 catggaggga tgcgggatcg tttcgtcgcg ccgtccacag accctctcgg ttgaggagtg
       61 ggcgtgcctg gcgcgtctcc tggcgccgca gctcgggtcc gaggctcgcg ggcggaggcg
      121 gctggagtga gggcggcgcg cctggcggca ccggccaagg tcaacctctg cctggccgtg
      181 atcggccgcc gaccggacgg ctaccacgag ctggtcaccg acatggtgct tttggagctg
      241 gccgatgagc tcctcctgct gcccggctgc gcgggactgc gcgtctccgg cgccaccgcc
      301 gccggcgtcc cggtgtcgcc gtcgcagaac ctcgcctggc gcggcctgct ggccgccgtg
      361 ggcggggcgc cggcggccgc ctgcctggag ctggacaagc ggatcccggc ggcggccggc
      421 ctgggcggtg gctcgtcgga tgcggccgcc gggtggcgtc tcgggcggct gctggccggc
      481 cgcgacgacc caccgactgg gtccgaggtc gcggcgctgg tcgcggtcgg cgcggacgtg
      541 ccgttcttcg ccgccacggt cgccgcggcg agggtcaccg gcgtcgggga ggatgtggcg
      601 cccatcgccg tcccggccac cgccgaggtc gtcctggcgg aggcgccaga cggcctgtcc
      661 acggccgccg tcttcgcgga gctgcgcgcc ggcgaatgga gcaccgagcc ggacccggag
      721 caaaacgacc tgctggccgc ggcacgccgg ttgcgccccg agatcgacga gctctttcgc
      781 atggtgacgg ccgccggcgg cgctccgcgc ctatccggct cgggacccac cgtcttcagc
      841 cttaccgatg accccgagcg ggccgacgcg gtggtccgac ggctgctgcg cgccggcgta
      901 cgcgccagcc gcacgcggct gcgacgcgaa ccggctagca tcgagctgct cgaccagcaa
      961 gacggccagc acgaggaggt ctgacaacgc aggtgcgcac ccaggtggca accaatgcgg
     1021 cgccggccgc catcggtccc tacagccagg ggatccgcat gggagagctg gtcttcaccg
     1081 ccgggcagat cgggctcgat ccggccagcg gcgagctggc ggaaggcgtg accgcgcagg
     1141 ccgagcgcgc gctgcgcaac gtggccgcca tcctggacgc ggccggcagc tccctggagc
     1201 gggtggtgaa gacgaccatc ttcctggccg acatgaacga cttcaacgcg gtgaacgagg
     1261 tgtacgccac ccacttcgcc acgccgtacc cggcccgttc cacggtggcg gtggccggcc
     1321 tcccgcgcgg ggcgctcatc gagatcgagg ccatcgccct ggccggagag ggctgagcgg
     1381 cgagggattg cggttgacca cgctcgcgtg gcgcggatac catcggcgcc accccatgcc
     1441 caacctggag cttcgtctca cttggtagcc accgcggcca tcgtcctggc cgccggacag
     1501 ggaacccgga tgcggtcccg gctgccgaag gtcctccatc cgctggctgg acgcccgatg
     1561 ctggaccacg tgctcgacgc gctggccgcc gccggcgtcg gccaccgggt ggtggtcacc
     1621 ggccacggcg cgcagcaggt cgaggcaacg ctcgacgacc gcgttccgac cgttcgccag
     1681 gacccgcagc ttggcacggc cgacgccgtg cgctgcggcc tctcgcgggt gccgcccgag
     1741 gcccggcacg tggtggtcac catgggtgac gttccgctcc tgcccgccga gctcttccag
     1801 aggctgctgc tcgagcaggc cgagggcgag gcgacgatcg cgctgctctc cgcccgcctg
     1861 ccggagccgg tcggctacgg tcgcgtcgtc cgcgcgcagg acgggtcagt gcgcgccatc
     1921 gtggaggagc gcgacgcgga cgaggcgacc cgctccctgg acgaggtgaa cgtcggtacc
     1981 tactgcttcg acgcggcctg gctgcgggcc aacgtcggca acgtgccggc gtccgcctcg
     2041 ggcgagtact acctcaccga cctggtggcg ctggcgacgg ccggcggtcg acgcgtagcg
     2101 gtggtgagcg ccccgcgacc ggagctgacc accggcatca acgaccgcgt ggcgctggcc
     2161 gaggccgagc ggctgctgcg tgccgagatc gcggaacgcc acatgcgcaa cggcgtgacc
     2221 atcgtggacc cggccaacac cttcatcgac gcgggggtgg agatcggcca ggacgtgcgg
     2281 atcgagccgt ggacggtgct cagcgggacc accgccatcg cgcaggacgc ggtgatcggg
     2341 ccgaatgcca cggtgcgcga ctcacggatc gggccgcgga ccagcgtgtg ggccagcgtc
     2401 atcgagtcct ccgaggttgc cgaggacgtg gagatcggac cgtacagcca cctccgacca
     2461 ggctgccgca tcggtccacg ctgccgcatc ggcaacttcg cggagctgaa gcagacgagg
     2521 atggggtccg gcacgcagca gcaccacttc agctacctgg gcgacgcgca ggtgggccag
     2581 gacgtgaaca tcggggccgg cgccgtgacc gccaactacg acggggccca gaagcacccc
     2641 accatgatcg gcgacggagc cttcatcggc gtggacacca tgctgcgcgc gccgatcacc
     2701 atcggtcc
//
LOCUS       DATACA010000006         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_338, whole genome shotgun sequence.
ACCESSION   DATACA010000006 DATACA010000000
VERSION     DATACA010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VHK28_00145"
     CDS             <1..748
                     /locus_tag="VHK28_00145"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX2140670.1"
                     /translation="LCLVDEAEAAAARVGFPLVIKSPLEGTTGVAYLDRPGEVAGAIR
                     AWQERYGVGAQELPILQQRIVGPGFGVFATYQEGRCVRIAAHGRVREFPPDGGESTCC
                     QLVHDPELLASGRRLLDAMGWHGVAMVELKRHDADGRLYLMEVNPKFWGSLDLMLAAG
                     ADFPGDVVRIAAGERLEEAQPPTGDLRFCWPLGNDLRHLLARPSSWRSVLRDWTGRAR
                     TNLRPSDPLPHLVELAGTVGRAVLRRGAER"
     gene            745..1404
                     /locus_tag="VHK28_00150"
     CDS             745..1404
                     /locus_tag="VHK28_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2140671.1"
                     /translation="MSRRGLFHVHTWHSFDGLLPPWAYLAHARRAGLDFLCVTDHNTL
                     AGARAVAAANRDPSLEVVIGAEYATERADVIGLFLREEVSSRRFDEVVSEIHAQGGLA
                     VLPHPFRHRAGVLDDEIFAAVDAVEVFNARSSVDENALALAAAVRHDKPQLAGPDSHT
                     LWELLRGGTLVTLEGDQPLREALLSAPRSFSTRPTSRNVRRYSQVVKRVRRRLRLPGA
                     G"
BASE COUNT          191 a          561 c          541 g          219 t
ORIGIN      
        1 cctctgcctc gtggacgagg cagaggcggc cgcggcgcgg gtcggcttcc cgctggtcat
       61 caagtcgcca ctggagggaa cgaccggggt cgcctacctg gaccgcccgg gtgaggtggc
      121 cggggcgatc cgggcctggc aggagcggta cggggtcggg gcgcaagagc ttcccatcct
      181 tcagcagcgg atcgtggggc ccgggttcgg cgtgttcgcc acctaccagg agggccgctg
      241 cgtgcggatc gccgcgcacg ggcgcgtgcg ggagttcccg ccggatggcg gcgagagcac
      301 atgctgccag ctggtccacg atcccgagct gctggccagc ggccggcgcc tgttggacgc
      361 catgggctgg cacggcgtgg ccatggtcga gctgaagcgc cacgacgcgg acgggcgcct
      421 gtacctgatg gaggtgaacc ccaagttctg gggctcgctg gacctcatgc tggctgccgg
      481 cgcggacttc ccgggggacg tggtgcgcat tgcggccggg gagcgcctgg aggaggcgca
      541 gccgcccacc ggcgacctgc ggttctgctg gccactgggc aacgaccttc gacacctgct
      601 ggcccggccg tcgtcgtggc gctccgtcct gcgcgactgg accggccggg caaggaccaa
      661 cctccggccc tccgacccgc tgccccacct ggtcgagctg gccggcaccg tcggtcgcgc
      721 cgtgctgcgc cgcggggcgg agcgatgagt cgccgcggtc tgtttcacgt ccacacctgg
      781 cactccttcg acgggctgct gccgccctgg gcctacctgg cccacgccag gcgcgccggg
      841 ctggacttcc tgtgcgtcac cgaccacaac accctggccg gagcgcgtgc cgtcgccgcc
      901 gccaaccgcg atccctccct ggaggtggtg atcggcgcgg agtacgcaac tgagcgtgcg
      961 gacgtgatcg gcctcttcct gcgagaggag gtttcctccc gccgcttcga cgaggttgtc
     1021 tccgaaatcc acgcccaggg cggcctcgcc gtcctgccgc atcccttccg gcaccgcgcc
     1081 ggggtcctgg acgatgagat cttcgccgcg gtcgacgccg tggaggtgtt caacgcgcgc
     1141 tcgagcgtcg acgagaatgc cctcgccctg gcggcggccg tacgccacga caagcctcag
     1201 ctggccggac ccgactcgca caccctctgg gagctgctgc gcggcggcac gctggtcacg
     1261 ctggagggcg accagccgct gcgcgaggcg ctgctgagcg ctccgcgcag cttcagcacc
     1321 cgacccacct cccgcaacgt ccgacgctac agccaggtcg tcaagcgcgt ccggcggcgg
     1381 ctgaggctgc ccggcgccgg ctgacctggg gctcagtcgc ggaagcggta gacccgcgcg
     1441 gcctcctccc cgtgcccgtc gtgcaccacc gccaccggct ccggttccac cagcgacagg
     1501 tacgcctcca gc
//
LOCUS       DATACA010000007         5441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1285650, whole genome shotgun sequence.
ACCESSION   DATACA010000007 DATACA010000000
VERSION     DATACA010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285650"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VHK28_00155"
     CDS             complement(<1..370)
                     /locus_tag="VHK28_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140672.1"
                     /translation="MALSADLAPIELYTADSRIVGWVAANGRRVTDLLNEQDELRLWR
                     PSPGPLEDHSIPAPAFVPGSVRDPAPPEEAGEWQSLVTDQVILAMPPEWRASRQLRLH
                     RKLRRVALGAGPFNVTGNLHI"
     gene            612..1097
                     /locus_tag="VHK28_00160"
     CDS             612..1097
                     /locus_tag="VHK28_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140673.1"
                     /translation="MNRTARILAAAAVLMLLVTATAIGAPSDRGQERRAPVASSHEPQ
                     TPEDVETADEPEDEAPSQAKLEEVATRLNATPEEIASLAEVYGLGGAVRILAWADAAP
                     NHEASDITALFDSGMGWGEIARQLNEEDPELDLHPGIGQIMGNGGGHGRENAPGQQKK
                     P"
     gene            1119..1454
                     /locus_tag="VHK28_00165"
     CDS             1119..1454
                     /locus_tag="VHK28_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008027784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEX2140674.1"
                     /translation="MIDFDHFAQVDMRVGRIVAAEPFPEARKPSYRLRIDFGPEIGER
                     VSSAQLTVTYPEPDKLIGRQVVAVLNFPPRRIAGLASEVLVLGAMGQGGNVWLLEPSP
                     EAPLGSRIG"
     gene            1533..1955
                     /locus_tag="VHK28_00170"
     CDS             1533..1955
                     /locus_tag="VHK28_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140675.1"
                     /translation="MLTGPAPDGMPDPTLAPPQIARPGDPFARLRVIHFVARLRRNAT
                     HQLRDVVAALNAAYLDWYFSEKVLLAELVQLQADWSISFHGEDRIVLDRNERGHTLLI
                     VDSTKMASFLVAEARRAAEDCEEELRRFTLGDGVSPDN"
     gene            complement(1952..2602)
                     /locus_tag="VHK28_00175"
     CDS             complement(1952..2602)
                     /locus_tag="VHK28_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2140676.1"
                     /translation="MARQQVLVIGLGRFGSAVARELERLGHEVLAVDANEQAVNEIAP
                     DVTHALQLDASDEDALRSAGADQFQTGIVAISSAVEPSIFATMVLKRLGVRNVIAKAG
                     SPLHADILSRIGADRVVLPEHETGIRLAHSFNVPNVIDYLDVGPSFGIEKVRPPESFI
                     GRSIGELDLKTRMGVTPIALRRGQSVIVNPAREERIAKGDELILIGRDDQLGDLRA"
     gene            complement(2642..4075)
                     /locus_tag="VHK28_00180"
     CDS             complement(2642..4075)
                     /locus_tag="VHK28_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEX2140677.1"
                     /translation="MTVQGRRPGDRRVRVARTRPEEIVLAPIRRARTPLSPAKSLVLA
                     FVAFISIGTVLLSLPVASASGEWTDFTAALFTATSAVCVTGLVVVDTGTYWSPAGQVI
                     ILLLIQVGGFGIMAGSTLLLYLLAGRRTTLRDRVVVQESLGGLQVGSVTSLLRRIALF
                     TLVVEGAGAVILSISFMLGPEAGPPGHPLGMWWGVFHSVSAFNNAGFDLTGGYRSLTP
                     FVDDWAVLGTIAILLILGGLGWAIVGDALGIRRWSRLALETKIVLVTTVGLLVAGTLV
                     IGALEWSNPATLGALPAEQRLLNAAFESATLRTAGFTALDTGSFVEASLFMVMALMFI
                     GGASGSTAGGIKVNTFGLLGAVITSSIRGRPSAEAFGRRIPHGIVYRALAVALLSIAF
                     VFLTGLGLAVTTNATFVQTLFEAVSAFGTVGASTGITPELNDRARIITAIAMFVGRLG
                     PITLVLALSARVRPVPYRPAVETIRIG"
     gene            complement(4129..5082)
                     /gene="thyX"
                     /locus_tag="VHK28_00185"
     CDS             complement(4129..5082)
                     /gene="thyX"
                     /locus_tag="VHK28_00185"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2140678.1"
                     /translation="MPTTTDLSARLIFPTKEIVEEYYLPLIYTACRTCYSEQLPDQIW
                     DKAVSRQVADEKQQGLVRKVMESGHGSTIEHVNFTFAISGVTRTLSHQLVRHRAGTAF
                     DQQSQRYVSFKRRDNYTVPDSITKGDLPDDLSGRFRQAIDDNLELYGQLLQAEVPAED
                     ARFIFPNAMQTNLIMTVNLRQLIHMSGLRLCTMAQWEIRQLFKQIRHEIFRVSPFFGS
                     FLAPKCVPLGYCDEMGNRDEHCRIRPHRDTVMAVWEAYRGGQLTETDGPVVPETSPFR
                     PRPRRIPVPVAPGEPAVETMPAPHGEPPQAAIGQAGGSDRV"
     gene            complement(5177..>5441)
                     /locus_tag="VHK28_00190"
     CDS             complement(5177..>5441)
                     /locus_tag="VHK28_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140679.1"
                     /translation="AARPFAAPGGEVTLAFSVGVAFMRPPQELTDEELVAAAEEALAS
                     ARAAGGNRIAFDRLHGLVRLEERREEERQLPPAVGDVEASEAS"
BASE COUNT          875 a         1875 c         1904 g          787 t
ORIGIN      
        1 tgatgtgcag gttgccagtc acgttgaacg ggcctgcgcc cagcgccacg cggcgcagct
       61 ttcggtgcag gcgcagctgg cgagaggcgc gccactcggg gggcatggcc aggatcacct
      121 ggtcggtgac cagcgactgc cactccccgg cttcctccgg tggggcggga tcacggacgc
      181 tgcccgggac gaaggcgggt gcggggatgg agtggtcttc caatgggccg gggctcgggc
      241 gccacagccg cagctcatcc tgctcgttga ggaggtcggt gacccgccgg ccgttggcgg
      301 cgacccatcc cacgatccgc gagtcggcgg tatacagctc gatgggggcc aggtcggcgg
      361 agagggccat tgccgacgag ccgttactcg tcgcaacacc actaccgcgg cgccagttcc
      421 agaaggtcac gtgccgttct ctcgtcagat gggtacggcc gcggggcagt cgcggctcgt
      481 tggccaaggc tagtctcggg cggggagctc accaaccgcc cgacagtact aggcggggtt
      541 gttgccgagc cgcaacggtt aggcatcatg ggactaacac ctcacgattc tgatgcgtta
      601 caactagcgc catgaacagg accgcccgca tcctggccgc cgcagccgtg ctcatgctgc
      661 tggtgacagc taccgccatc ggcgcgccgt cggaccgcgg ccaggagcgc cgcgcccccg
      721 tcgcctcgtc gcacgagcca cagactcccg aggacgtgga gacggcggac gagccggagg
      781 acgaggcgcc gtcgcaggcc aagctggagg aggtggcgac caggctgaac gccacgcccg
      841 aggagatcgc ctcgctggcc gaggtctacg gactgggtgg agcggttcgg atcctggcct
      901 gggcggatgc ggcccccaac cacgaggcat ccgatatcac cgccctcttc gattcgggga
      961 tgggctgggg cgagatcgcg aggcagctga acgaggaaga tcccgagctc gaccttcacc
     1021 cgggcatcgg ccagatcatg ggcaacggcg gcggtcacgg ccgcgagaac gcaccgggac
     1081 agcagaagaa gccctagcag cttccatctc ggccccgcgt gatcgacttc gaccacttcg
     1141 cccaggtaga catgcgcgtc ggccgcatcg tggcggccga gccctttccg gaggcgcgca
     1201 agccctccta ccgactgcgc atcgacttcg ggcctgagat cggtgaacgc gtcagctccg
     1261 cgcagctgac ggtcacctac ccggagcccg acaagctcat cggtcgccag gtcgtcgcgg
     1321 tgctgaactt cccgccgcgg cgcatcgcgg ggctcgccag cgaagtgctg gtgctgggcg
     1381 ccatgggcca gggcgggaac gtgtggctgc tggagccgag cccggaggcg cccctgggct
     1441 cgaggatcgg gtaggaccca cgacacgccc gttcgccaaa atcatcaggc ggcggcacac
     1501 tgatacctgt gacccagccg accactgaac ccatgctgac cggtcccgcg cccgacggca
     1561 tgcccgaccc tacccttgcc ccgccgcaga tcgcccgccc cggcgacccg ttcgcccgcc
     1621 tgcgggtgat tcacttcgtc gcgcggctgc gccgcaacgc cacccaccag ctgcgcgacg
     1681 tggtcgccgc cctgaacgcg gcctacctgg actggtactt cagcgagaag gtgctgctgg
     1741 ccgagctcgt ccagctgcag gccgactggt cgatcagctt ccacggcgaa gaccgcatcg
     1801 tgctggaccg caacgagcgg ggccacaccc tgctgatcgt ggactccacg aagatggcca
     1861 gcttcctggt ggccgaggcg cgacgcgcgg ccgaggactg cgaggaggag ctgcgccgct
     1921 tcacgctcgg cgacggggtc tcgccggaca actaggcgcg caggtccccg agctggtcat
     1981 cgcggccgat caggatcagc tcgtcgccct ttgcgatgcg ctcctcgcga gccgggttga
     2041 cgatcacgct ctgccctcgc cgcagggcga tcggggtgac gcccatgcgg gtcttcaggt
     2101 ccagctcacc gatgctgcgt ccgatgaacg actccggcgg ccgcaccttc tcgatcccga
     2161 aactggggcc gacgtccagg tagtcgatca cgttcgggac gttgaacgag tgggccagcc
     2221 ggatcccggt ctcgtgctcg gggagcacca cccgatccgc gccgatcctg gacaggatat
     2281 cggcgtgcag cgggcttccg gccttggcga tcacgttgcg caccccgagc cgcttgagca
     2341 ccatggtggc gaagatcgag ggctcgaccg cgctgctgat ggccacgatg ccggtctgga
     2401 actggtccgc cccagcgctg cgcagggcgt cctcgtcaga ggcatccagc tggagggcgt
     2461 gggtcacatc gggcgcgatc tcgttgaccg cctgctcgtt ggcgtccacc gccagcacct
     2521 cgtgtcccag gcgctcgagc tcgcgggcca ccgccgagcc gaagcggccc aggccgatca
     2581 ccagcacctg ttggcgcgcc acgctgatcc tcgctccttc cttgtcatga accgatgcgg
     2641 actagccgat ccggatcgtc tcgaccgccg gccgataggg tacaggccgg acgcgggccg
     2701 acagcgccag gaccagggtg atggggccca gccggccgac gaacatggcg atggcggtga
     2761 tgatccgcgc tcggtcgttc agctccggcg tgatgccggt cgaggctccc accgtgccga
     2821 acgccgacac ggcctcgaac agggtctgca cgaaggtggc gttggtggtc acggccaggc
     2881 ccaggccggt caggaacacg aacgcgatcg acagcagggc cacggccagc gcgcggtaca
     2941 cgatgccgtg tgggatgcgc cggccgaagg cctccgccga ggggcggccg cggatgctgg
     3001 aggtgatcac cgctcccagc aggccgaacg tgttcacctt gatgccaccc gccgtggagc
     3061 cggaggcgcc gccgatgaac atgagggcca tgaccatgaa caggctggcc tccacgaagc
     3121 tgccagtgtc cagggcggtg aagccggcgg tgcgcagcgt ggcggactcg aacgcggcgt
     3181 tcagcaggcg ctgctcggcc ggcaacgcgc cgagcgtggc cggattgctc cactccagcg
     3241 ccccgatcac cagcgtcccg gcgaccagca gcccgaccgt ggtgaccagc acgatcttgg
     3301 tctcgagggc caggcgcgac cagcgcctga tgccgagcgc atcgcccacg atcgcccatc
     3361 ccaggccgcc gaggatgagc aggatggcga tggtgcccag caccgcccag tcgtcgacga
     3421 agggcgtcag gctgcggtag ccgccggtca ggtcgaagcc ggcgttgttg aaggccgaga
     3481 cggagtggaa gacgccccac cacatcccca gcgggtgtcc gggcggaccg gcctctggcc
     3541 ccagcatgaa gctgatggac aggatcaccg ccccggcacc ctccaccacc aacgtgaaga
     3601 gcgcgatgcg gcgcagcagg ctggtcacgc tgcccacctg gagtcccccc agcgattcct
     3661 ggacgaccac ccggtcgcgc agggtcgtgc gcctgccggc gagcaggtac agcagcaggg
     3721 tggagccggc catgatcccg aagccgccca cctggatgag gagcaggatg atgacctggc
     3781 ccgccgggct ccagtaggtg ccggtgtcca ccaccaccag cccggtgacg cacaccgccg
     3841 aggtggccgt gaagagcgcg gcggtgaagt cggtccactc tccggaggcg gatgccacgg
     3901 ggagcgagag cagcacggtc ccgatgctga tgaaggccac gaaggccagc accaggctct
     3961 tcgccgggga gagcggcgtt cgtgcccgac ggatcggcgc cagaacgatc tcctcgggcc
     4021 gggtgcgcgc cacccgtacg cggcggtcgc cgggacgtcg cccctggacg gtcatcgatc
     4081 agcgccgcgc acgcacacgc gcgtacggta gcgcgaatcg gcccgcgctc agaccctgtc
     4141 ggagcctccg gcctgaccga tggccgcctg cggcggctcg ccgtgcggcg ccggcatggt
     4201 ctccacggca ggctccccgg gagcgaccgg caccgggatg cgccgcgggc ggggacggaa
     4261 gggagaggtc tcggggacga ccggtccatc ggtctccgtg agctggcccc cccggtaggc
     4321 ctcccagacg gccatcaccg tgtcccggtg cgggcggatg cggcagtgct cgtcccggtt
     4381 gcccatctcg tcgcagtagc ccagcggcac gcacttgggt gccaggaagc tgccgaagaa
     4441 gggcgacacg cggaagatct cgtggcgaat ctgcttgaag agctgccgga tctcccactg
     4501 cgccatggtg cacagccgca gcccgctcat gtggatcagc tggcgcaggt tgacggtcat
     4561 gatcaggttg gtctgcatgg cgttggggaa gatgaagcgg gcatcctcgg ccggcacctc
     4621 ggcctgcagc agctgaccgt acagctccag gttgtcatcg atcgcctggc ggaagcggcc
     4681 tgaaaggtcg tcgggaaggt cacccttggt gatcgaatcg gggaccgtgt agttgtcgcg
     4741 gcgcttgaag gacacgtagc gctgcgactg ctggtcgaag gcggttccgg cccgatgccg
     4801 caccagctgg tgcgagaggg tgcgggtcac gccgctgatg gcgaaggtga agttcacatg
     4861 ctcgatggtc gagccgtggc cggactccat caccttgcgc accaggccct gctgcttctc
     4921 gtccgccacc tggcggctga ccgccttgtc ccagatctgg tccggcagct gctccgagta
     4981 gcaggtgcgg caggcggtgt agatcagcgg aaggtagtac tcctccacga tctccttggt
     5041 gggaaagatg agccgggcag agaggtcggt ggtggtcggc atgcgtccgg cgaggatagc
     5101 accggcccgc cgcgtcgcga gccggttgtc cacaacatgt tgcggtgtgg ataaccgatc
     5161 gccgctatgt tgtgtttcag ctggcctccg aggcctccac gtccccgacc gccggcggca
     5221 gctggcgctc ttcctcccgg cgttcctcga gacgtaccag gccgtgcagc cggtcgaagg
     5281 cgatgcggtt tccgcccgcc gcacgggcgg aggccagcgc ctcctcggcc gccgccacca
     5341 gctcctcgtc ggtcagctcc tgcggcggcc gcatgaacgc cacacccacc gagaaggcca
     5401 gggtgacctc gccaccgggc gcggcgaacg gccgagcagc c
//
LOCUS       DATACA010000008         4056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_562771, whole genome shotgun sequence.
ACCESSION   DATACA010000008 DATACA010000000
VERSION     DATACA010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562771"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHK28_00195"
     CDS             <1..201
                     /locus_tag="VHK28_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter, permease protein
                     PstA"
                     /protein_id="HEX2140680.1"
                     /translation="GFNLAYHNPVEQLFGRYTALPTTIYDWAGNPASFAGNVYAAIIV
                     LLVMLLGVNALAVYLRNRYERR"
     gene            243..1034
                     /gene="pstB"
                     /locus_tag="VHK28_00200"
     CDS             243..1034
                     /gene="pstB"
                     /locus_tag="VHK28_00200"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX2140681.1"
                     /translation="MATSAAAVAEERVFELRDVSCFYGSFRAVRHVTLDIPRNRITAI
                     IGPSGCGKSTLLRTLNRMNDLVEGARVDGRILYHGVDVYDPQVDPVEVRRRIGMVFQK
                     PNPFPKSIYDNVAFGPRVARYRGDLDELVERSLRRAALWDEVKDKLGSPGTALSGGQQ
                     QRLCIARAIAVEPDVILMDEPCSALDPIATLRIEELMNELKERYTIVIVTHNMQQAAR
                     ASDHTAFLTMGEDRSGYLVEMAPTDQLFTMPQHKLTEDYITGRFG"
     gene            1040..1762
                     /gene="phoU"
                     /locus_tag="VHK28_00205"
     CDS             1040..1762
                     /gene="phoU"
                     /locus_tag="VHK28_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX2140682.1"
                     /translation="MEERARDREQRLPKRADGEVAPSIRRAFEEHLHETRDSVLRLGG
                     LVEEQLQRAGRALVERDVALADRVRWGDAEVNELQREVNLGIAALIARQGPMARDLRE
                     LLALYHAAAELERMGDYATNIAKLAQQLAAEPELPLFHQIPRMEELCREQLRAAMRAL
                     VDLSEEAARAVCARDDEIDHLYNSVYEDALRLMESSPERARQATHMLFAAHHLERLGD
                     RVTNIGEDVVFLATGRIEDLNP"
     gene            1777..2499
                     /locus_tag="VHK28_00210"
     CDS             1777..2499
                     /locus_tag="VHK28_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2140683.1"
                     /translation="MKRRILVVEDDRVLRQALTYNLSREGYEVHVAVDGEQALAAGRD
                     PGLDLVLLDLMLPGMSGLEVLRVLRGEGVTTPVVILSAKGEEVDRVVGLKVGADDYVA
                     KPFSRPELLARIEAVLRRQRRAASPADQRRDVVRSGNLELDPSSRTASFGGRPLSLTT
                     RQFDLLAHLASHPGRIFTREQLLIRLWGLDYEGDARTVDVHVSWLRSKLRELDGHNYF
                     RTVRGVGYAFDPAPETAATANR"
     gene            2496..3575
                     /locus_tag="VHK28_00215"
     CDS             2496..3575
                     /locus_tag="VHK28_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2140684.1"
                     /translation="MTADASGAEAFEALLAQDGEVLLLLVDDRLRILRAGAEAALLAE
                     RPVEELAGMSLIAAFGSAQLDGLARQGMLEGKPSRGEAEMGRLGRRSFAVEAVPLRSG
                     GLVLALQETTTLRRIERVRRDFVANISHELRTPIASVKLLAETLSEGAAADPDTARDF
                     AVQIAREVDHLAQLVDELLDLSMIESGETQLVVRPQRPAVILAGLSERIRPVAERRSV
                     AVRILPAPPKGDEVRALVDVDRIGQALLNLTHNAVKYSPPHGEVRLGWEVRPEVVRFL
                     VSDDGPGIPQVHQARVFERFYKVDRSRARSAPDEAGTSGSAGLGLAIVRHIAEAHGGA
                     VGVDSQEGAGATFWLDVPMDGRDAG"
     gene            3589..>4056
                     /locus_tag="VHK28_00220"
     CDS             3589..>4056
                     /locus_tag="VHK28_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2140685.1"
                     /translation="MDLTDRVAVITGATGDLGRVAARRLLDAGASLVLVGTARRRLEE
                     LAGTLPSADGRVKLVEADLTSEEGAASLAEALDNGPGRADLLLHGIGGWAGGESLLDV
                     SLDTVRGMVDAHLWSTTYTVRAVVPRMVAGGWGRVVVVSTPGSARPGQGQAAYA"
BASE COUNT          628 a         1362 c         1458 g          608 t
ORIGIN      
        1 ggcttcaacc tggcatacca caacccagtg gagcagttgt tcggtcgcta caccgcgctg
       61 ccgacgacca tctacgactg ggccggtaac cccgcctcat tcgccggcaa cgtgtacgcc
      121 gccatcatcg tgttgctggt gatgctgctg ggggtcaatg ccctggcggt ctacctgcgc
      181 aaccggtacg agcgcaggtg acaataccga tggtgatcgg acagcagctg gaaccagcac
      241 ccatggcgac ctcggcagcc gcggtggccg aggaacgcgt cttcgagctg cgtgacgtct
      301 catgcttcta cggatcattc cgggcggtgc gccacgtcac gctggacatc ccgcgcaacc
      361 ggatcacggc catcatcggg ccgtccggct gcggcaagag cacgctcctg cgtacgctca
      421 accggatgaa cgacctggtg gaaggtgcgc gcgtcgatgg ccgaatcctg taccacggcg
      481 tcgacgtcta tgacccgcag gtcgatccgg tcgaggtgcg gcgccggatc gggatggtgt
      541 tccagaagcc gaacccattc cccaagtcga tctacgacaa cgtcgccttt gggccgcgtg
      601 tggctcgcta tcgcggggac ctggacgagc tggtggaacg atccctccgt cgagccgccc
      661 tctgggacga ggtcaaggac aagctgggct ctccgggcac cgcgctttcg ggcggccagc
      721 agcagcggct gtgtatcgcc cgcgccatcg cggtagagcc ggatgtcatc ctcatggacg
      781 aaccgtgcag cgcgcttgat cccatcgcca cgctgcgcat cgaggagctc atgaacgagc
      841 tcaaggagcg ctacaccatc gtcatcgtca cccacaacat gcagcaggcc gcacgcgcca
      901 gcgaccacac cgccttcctg accatggggg aggatcggtc cgggtacctt gtcgagatgg
      961 cgcccacaga ccagctcttc accatgccgc agcacaagct caccgaggac tacatcaccg
     1021 gacgcttcgg atgagcacca tggaggagcg cgcccgcgat cgtgagcaac ggctgcccaa
     1081 gcgcgccgac ggtgaggtgg cgccatccat ccgtcgagcg ttcgaggagc acctgcacga
     1141 gacgagggac tccgtcctgc gcctcggcgg cctggtggag gagcagctgc aacgtgccgg
     1201 acgcgccctc gtcgagcggg acgtggcgct cgccgaccgt gtgcggtggg gggacgcaga
     1261 ggtcaacgag ctccagcgag aggtcaacct gggcatcgcg gcactgatcg cccggcaggg
     1321 accgatggcg cgcgatctgc gtgagctgct ggcgctctac catgccgctg ccgagctgga
     1381 gcggatgggc gactatgcca ccaacatcgc caagctggcc cagcagctgg ccgccgaacc
     1441 cgagctaccc ctcttccacc agatcccgcg catggaggag ctgtgtcgcg agcagctgcg
     1501 cgccgcgatg cgtgccctgg ttgacctcag cgaggaggcg gcgcgcgcgg tatgtgcgcg
     1561 cgacgacgag atcgatcacc tgtacaacag cgtctacgag gatgcgctgc gcctcatgga
     1621 atcctcgccc gagcgcgcgc ggcaggctac gcacatgctg ttcgccgctc accacctgga
     1681 gcggctcggg gaccgggtga cgaacatcgg ggaggacgtc gtgttcctgg ccaccggccg
     1741 catcgaggat ctcaacccct gaccgggcgc ggaccgatga agcggcgcat cctggtcgtc
     1801 gaggacgatc gcgtcctccg ccaggccctg acctacaacc tgtcacggga aggctacgag
     1861 gtccacgtgg ccgtcgacgg cgaacaggca ctggcggcgg gtcgggatcc ggggctggac
     1921 ctcgtactgc tggacctgat gctgcccggc atgagcggcc tggaggtgct tcgcgtcctg
     1981 cgtggcgagg gggtgacgac gccggtggtg atcctctccg ccaagggcga ggaggtcgac
     2041 cgcgtggtcg gcctcaaagt gggagcggac gactacgtgg ccaagccctt cagtcggccg
     2101 gagctgctgg ccaggatcga ggcggtgctc cgccgtcagc gtcgcgcggc cagccccgcc
     2161 gaccagcgga gggacgtggt ccgcagcggc aacctcgagc tggacccgtc cagccgcacg
     2221 gcctccttcg gtgggcggcc actgtcgctc accacccgtc aattcgacct gttggcgcac
     2281 ctcgcctcgc accccggtcg catcttcacc cgggagcagc ttctcatccg gctgtggggc
     2341 ctggactacg agggagatgc gcgcaccgtt gacgtgcacg tgtcctggct ccgttcgaag
     2401 ctgcgcgagc tggacgggca caactacttc cgaacggtgc gaggcgtcgg gtacgccttc
     2461 gatccggccc cggagacagc ggcgacggcg aaccgatgac tgccgacgcc agcggcgccg
     2521 aggcgttcga ggcgctcttg gcgcaggacg gcgaggtgct gctgctgttg gtggacgatc
     2581 ggctgcggat cttgcgcgca ggcgcggagg ccgccttgct ggcggagcgc ccggtcgagg
     2641 agcttgccgg catgagcctg atcgccgcat tcggctccgc ccagctggac gggcttgctc
     2701 gccaggggat gctggaaggc aagccctccc ggggcgaggc ggagatggga cgcctggggc
     2761 gtcgctcgtt cgcggtggag gccgtcccgc tgcggtcggg cggcctggtg ctggccctgc
     2821 aggagacgac gactctgcgg cgcatcgagc gcgtgcgacg cgacttcgtg gcgaacatca
     2881 gccacgagct tcgtacgccg atcgcatcgg tcaagctcct ggccgagacc ttgtccgagg
     2941 gtgcggccgc cgaccccgac accgcgcgcg acttcgcggt ccagatcgcc cgggaggtgg
     3001 accacctggc gcagctcgtg gacgagctgc tggacctgtc catgatcgag tcgggcgaga
     3061 cccagctggt ggtgcgcccg cagcgtccgg ccgtgatcct ggccgggctg tccgagcgga
     3121 tccggccggt cgccgaacga cggagcgtcg ccgtgcgcat cctgccggcg ccgccgaagg
     3181 gggacgaggt acgggccctg gtcgacgtcg atcgtatcgg acaggccctg ctgaacctca
     3241 cgcacaatgc cgtgaagtac agccctcccc atggcgaagt gcggctgggc tgggaggtgc
     3301 ggccggaggt cgtgcgcttc ctggtgagcg atgacgggcc cggcattccc caggtccacc
     3361 aggcgcgcgt cttcgagcgc ttctacaagg tggatcgatc gcgggcgcga agcgcgccgg
     3421 acgaagcggg cacctcgggg agtgccggcc tcgggcttgc gatcgtgcgc cacatcgccg
     3481 aggcccacgg aggcgcggtg ggcgtcgaca gccaggaagg tgctggcgcc accttctggc
     3541 ttgacgtgcc gatggacggg cgcgacgcgg ggtgagacga tcgggagcat ggacctgacc
     3601 gatcgagtgg ccgtcatcac cggggcgacc ggggacctgg gccgcgtggc ggcccgtcgc
     3661 ctgctcgacg cgggcgcctc cctggtcctg gtgggcaccg ctcgccggcg cctcgaagag
     3721 ctggccggca cgctaccctc cgccgacggc cgggtgaagc tggtggaggc ggacctgacc
     3781 agcgaggagg gggccgcatc gctggccgag gcgctcgaca acgggccggg acgggccgac
     3841 ctgctgctgc acggtatcgg cggctgggcc gggggcgagt cgctccttga cgtgtcactc
     3901 gacacggtgc gcggcatggt ggacgcccac ctgtggagca cgacctacac cgtgcgcgcc
     3961 gtcgtgccgc gcatggtggc cggcggctgg ggtcgcgtcg tggtggtctc cacgccgggc
     4021 tcggccaggc ccggacaggg ccaggcggcg tatgca
//
LOCUS       DATACA010000009         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1125225, whole genome shotgun sequence.
ACCESSION   DATACA010000009 DATACA010000000
VERSION     DATACA010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125225"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VHK28_00225"
     CDS             <1..542
                     /locus_tag="VHK28_00225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2140686.1"
                     /translation="ISIRNIRKRDLASEIERAKLVYNEAWFNNWGFVRMTDAEFDHLG
                     RHLAEVADERLLFIAERGDEPIGFSLALPDVNEAFRAVPDGRLTHLGLPDGLARLYLA
                     ARHIRAFRFFTLGVRPAYQGRGIAEALILRTLDAALAAGYTGAEMGWTLDDNTRVNRV
                     IERSGAGRYKTYRIYEGPI"
     gene            complement(539..706)
                     /locus_tag="VHK28_00230"
     CDS             complement(539..706)
                     /locus_tag="VHK28_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140687.1"
                     /translation="MSVQDQNEDKRKTDVTDESAPANDVPEERDESADDDYPAGGALT
                     GEQEFAPDDKP"
     gene            complement(703..987)
                     /locus_tag="VHK28_00235"
     CDS             complement(703..987)
                     /locus_tag="VHK28_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2140688.1"
                     /translation="MTLFEGQVPDDREAALRDAFIENTSGPLPEGVHEMRLLRSPNVP
                     GQWGIVTRWRSPQLLAAYREAADPPIEVLAFRGADIEPVETEYEVITAEA"
     gene            1142..1918
                     /locus_tag="VHK28_00240"
     CDS             1142..1918
                     /locus_tag="VHK28_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140689.1"
                     /translation="MITELPGFPFPVVTTHGLEEKASRIAERTRDARAWMSDILGMEP
                     RLRVEVLGPGDFAERAELPALGLPHMNEEGTMFLAGYDTSTFDSSVAQTLKYLSPADR
                     AGFALVYGEPPSVQPFVDLLSLHELAHAYHMQRGWSFQELWMGELFCNLALQGYVANH
                     EPAVRAALETLPASAHHIPPEAQGVTSIGAMNAEDPLTYVWYEFRLQVAAIRLWDAGG
                     PELLREFYEQQQLAAQDNTLADASRLPAAIQWVRDAWPDV"
     gene            2013..2087
                     /locus_tag="VHK28_00245"
     tRNA            2013..2087
                     /locus_tag="VHK28_00245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2045..2047,aa:Lys,seq:ttt)
     gene            complement(2468..>3184)
                     /locus_tag="VHK28_00250"
     CDS             complement(2468..>3184)
                     /locus_tag="VHK28_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140690.1"
                     /translation="QNEISFLTWAGGEVGIMNPHARGRGAVLKQQLVRGAIFAMDAVR
                     AELPGVRFLHPEPLINVATDASRPHERHQAVTATEAQFEAWDMLAGRMAPELGGADRY
                     LDVLGANYYPDNQWALDGATLEADSPHRARLSTLLATLHERYGRDILISETGTEDGAR
                     GAWLREVSAEVDRAVGTGVPVGGICLYPVLNHPGWEDDRHCQNGIWDYPGVRGGRRAY
                     RPLVQHVRREQERLLRAVAY"
BASE COUNT          491 a         1115 c         1018 g          560 t
ORIGIN      
        1 gcatttccat ccgcaacatc cggaagcgcg acctcgcctc cgagatcgag cgggccaagc
       61 tggtctacaa cgaggcgtgg ttcaacaact ggggcttcgt gcgaatgacc gatgcggagt
      121 tcgaccacct cggccgtcac ctggcggagg tggccgatga gcgcctgctg ttcatcgcgg
      181 agcggggcga tgaaccaatc gggttcagcc tggcactgcc cgatgtgaac gaggccttcc
      241 gcgcggtccc ggacgggcgc ctcacccacc ttggcctgcc cgatggactg gcgcgcctct
      301 acctggccgc acgccacatc cgcgccttcc gcttcttcac gctcggcgtg cggccggcct
      361 accagggccg cggcatcgcc gaggcgctca tcctgcgcac gctggacgcg gcgctggcgg
      421 ccggctacac cggagccgag atgggctgga cgctggacga caacacgcgc gtcaaccggg
      481 tgatcgagcg ctccggtgct ggacgctaca agacctaccg catctacgaa gggccgatct
      541 agggcttgtc gtccggcgcg aactcctgct cgccggtcag ggcgccaccc gccggatagt
      601 cgtcgtccgc gctctcgtcg cgctcctcgg gcacgtcgtt ggctggcgcc gactcatcgg
      661 tcacatcggt cttgcgcttg tcctcgttct ggtcctgcac gctcatgcct ccgcggtgat
      721 gacctcatac tcggtttcaa ctggctcgat gtccgcgccc cggaaggcaa gcacctcgat
      781 cggggggtcg gccgcttccc ggtaggccgc cagcagctgt gggctgcgcc agcgggtcac
      841 gatgccccac tggcccggga cgttcgggct gcgcagcagc cgcatctcgt gcactccctc
      901 ggggaggggg ccggaggtgt tctcgatgaa ggcgtcgcgc agggccgcct cccggtcgtc
      961 cggcacctgc ccctcgaaca gcgtcatgac ttcctgttgc atacggctcg tgtcagcagg
     1021 agtcggtcca actcccagtg cagtttgcgg aagcagccgt gcgggccggt gctagccttg
     1081 gctgcggtcc cgcgcgcgcc gccgcgccgc ctcgccgccg cgctctcgag gaatggtacc
     1141 gatgatcacc gagctccccg gtttcccctt tccggtcgtc acgacacacg ggctcgagga
     1201 gaaggcgtcg cgcatcgccg aacgcacccg cgacgctcgc gcatggatgt cggacatcct
     1261 gggcatggaa ccgaggcttc gagttgaagt gttgggaccg ggggacttcg ccgagcgtgc
     1321 tgaactcccg gcgttgggcc tgccgcacat gaacgaagag ggcacgatgt tcctggctgg
     1381 gtacgacacc tccaccttcg actcgtccgt cgcgcagacg ctgaagtacc tttcgcccgc
     1441 cgatcgtgcg ggctttgcgc tggtctacgg tgagccgcca agcgtgcagc cgttcgtcga
     1501 cctgctttcg ctgcacgaac tggcccacgc ctaccacatg cagcgcggct ggtccttcca
     1561 ggagctgtgg atgggtgagc tcttttgcaa cctggcgctg caggggtacg tggcgaacca
     1621 cgagcccgcc gttcgcgcgg cactcgaaac tcttccggcc tcggcccacc acattcctcc
     1681 ggaagcgcag ggcgtgacca gtatcggtgc catgaacgcg gaggaccccc tgacctacgt
     1741 ctggtacgag ttccggttgc aggtcgcagc gattcgactg tgggatgccg gcggacccga
     1801 gctgctccgc gagttctatg agcagcagca gctcgccgcg caggacaaca cgctggctga
     1861 cgcgtcccgc ctgcctgcgg caatccaatg ggttcgggac gcttggccgg acgtctgatc
     1921 ccttgatcta ttgcgcggcg ggtgcgcggt gcttcgcggc tgcaacgaga tgtgatcctc
     1981 gtgaacggcc gctaacctga gcacgaaacg gtgggccgtt agctcagtgg cagagcaggg
     2041 gacttttaat cctcgtgtcg tgggttcgaa tcccacacgg ccctccacaa tttcatcctc
     2101 gaacctggcc ggctggtgcc ggctatgctc gctccagcac cggccgccat ccacgagatc
     2161 gtcggaggcg gcgctgacct caggaatcat caggctcttc gtcggcgttc acgcaagcgt
     2221 ggcagcggcg cgcctctggc ggtccagcct caccaggcct tgtgggccag acgcggcctc
     2281 ggaatatgct cagctcccac ccacagccag cggcgtaatc gccgccgcca cgatattccg
     2341 cagaaccatg ccacacggat ttgtccggtt cctcgaccca gattcggtac accttggcca
     2401 tcccccgcca atcagccaga ctgatgccat cgctcgcctt ggctgaggga tacagctgcc
     2461 gataccttca atacgcaacg gcccgcagca gtcgttcctg ctctcgccgg acgtgctgca
     2521 cgagcggccg atatgcgcga cgcccgcccc gcacgccggg atagtcccag atcccgtttt
     2581 ggcaatgccg gtcgtcctcc caccccggat ggttgaggac ggggtatagg cagatcccgc
     2641 ccaccgggac gccggtaccg accgcgcggt ccacctcggc gctcacctcc cgcaaccacg
     2701 caccgcgcgc tccgtcctcg gtccccgttt cagagatcag aatgtcgcgg ccgtaccgct
     2761 cgtggagcgt cgcgagcagc gtggatagtc gagcgcggtg aggactgtcg gcttcgagag
     2821 tcgcgccgtc gagggcccat tggttgtcgg gatagtagtt cgcgccaagc acgtcgaggt
     2881 accgatccgc gccgcccagc tcgggagcca tgcggcctgc gagcatgtcc catgcctcga
     2941 actgcgcctc ggttgccgtc accgcctggt ggcgctcgtg gggccgcgag gcatcggtcg
     3001 ccacattgat gagcggctcg ggatgaagga accggacgcc ggggagctcc gctcggaccg
     3061 catccatcgc gaaaatcgcg ccacgcacca gctgctgctt gagcaccgcc ccgcggcccc
     3121 gggcatgcgg gttcatgatg ccgacttcgc caccagccca cgtcaggaac gagatctcgt
     3181 tctg
//
LOCUS       DATACA010000010         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_161358, whole genome shotgun sequence.
ACCESSION   DATACA010000010 DATACA010000000
VERSION     DATACA010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161358"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..309
                     /gene="fusA"
                     /locus_tag="VHK28_00255"
                     /pseudo
     CDS             1..309
                     /gene="fusA"
                     /locus_tag="VHK28_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824066.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(305..1855)
                     /gene="fusA"
                     /locus_tag="VHK28_00260"
                     /pseudo
     CDS             complement(305..1855)
                     /gene="fusA"
                     /locus_tag="VHK28_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
     gene            complement(1863..2336)
                     /gene="rpsG"
                     /locus_tag="VHK28_00265"
     CDS             complement(1863..2336)
                     /gene="rpsG"
                     /locus_tag="VHK28_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2140691.1"
                     /translation="MPRRPTVPRKTKYADDLVGTALTLDQFTNKLMRGGKRQLASRIL
                     EDAIARCEKQAGRAGVEVMELALRNAMPLIEVKPKRVGGSTYQIPVEVRADRRVSLGM
                     RWLIEAARKRNGRSMAEKLSAEFLDASNNLGAAVKRREDTHRMAEANRAFSHFKW"
     gene            complement(2341..2763)
                     /gene="rpsL"
                     /locus_tag="VHK28_00270"
     CDS             complement(2341..2763)
                     /gene="rpsL"
                     /locus_tag="VHK28_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX2140692.1"
                     /translation="MPTISQLVRKGRKPAGKKVKAPALRRTLNTLKQTASEGRGAPQR
                     RGVCIQVMTRTPKKPNSALRKVARVRLVNGMEVTAYIPGIGHNLQEHSVVLVRGGRVK
                     DLPAVKYHIVRGTLDAAGVRDRKQGRSKYGAKSPGKGS"
     gene            complement(3236..3376)
                     /locus_tag="VHK28_00275"
     CDS             complement(3236..3376)
                     /locus_tag="VHK28_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140693.1"
                     /translation="MSILEGTRPDAAVACQDIGVVKSVTFAIEEPLIVQAAFDSEGAR
                     NL"
BASE COUNT          506 a         1145 c         1189 g          607 t
ORIGIN      
        1 cggctggccg aggaggaccc caccttccgc gtcagcaccg acgtggagtc gtcccagacc
       61 cgcatcgccg gcatgggcga gctgcacctc gaggtgctcg tcgaccgcat gctgcgcgag
      121 ttcaaggtgc aggccaacgt cggtcggccg caggtcagct accgcgaggc catccgcaag
      181 ccggtgcgcg ccgagggccg cttcgtgcgg cagacgggcg gcaagggcca gtatggccac
      241 gccatcgtgg agttcgagcc gctggagcgc ggtgtgggct tcgagttcga gtcgaaggtc
      301 gtgggtggcc gtactggccc ttgccgccgg tctgccgcac gaagcggccc tcggcgcgca
      361 ccggcttgcg aatggcctcg cggtagctga cctgcggccg accgacgttg gcctgcacct
      421 tgaactcgcg cagcatgcgg tcgacgagca cctcgaggtg cagctcgccc atgccagcga
      481 tgcgggtctg ggacgactcc acgtcggtgc tgacccggaa ggtggggtcc tcctcggcca
      541 gccgctgcag ggccagcgac atcttgtcct ggtccgcctt agtcttgggc tcgacggcca
      601 gctctatcac cggctcgggg aaggtgatgg cttccaggac gaccggcttc gactcatcgc
      661 acagcgtgtc gccggtggtc gtgttcttca ggccgaccgc ggcggcgatg tcgccggcgc
      721 tcaccgactc gatctcctcg cggtggttgg cgtgcagctg gagcaggcga ccgacgcgct
      781 ccttggtgcc cttggtcgag ttgtacacgt aggagccggt cttgagcgtc ccggagtaca
      841 cccggaagaa ggccagcttg ccgacgtacg ggtcggccgc gatcttgaag gcaagggcgg
      901 agaacggcgc gtcgggatcg gtctggcgga tcgtctcctc accgtcgcgc gggtccttgc
      961 cggtgaccgg cggcacgtcc atgggcgagg gcaggtagtc gatgaccgcg tccagcatgg
     1021 gctgcacgcc cttgtttttc agggcagacc cgcacaggac cggcacgatc ttgtaggcca
     1081 gcgtcccggc ccgcagcgcc ttgcggagct ggtcaggacc gatctcctcg ccctcgaggt
     1141 acttgtgcgc gatgtcgtcg tccaagtcgg ccagggcctc gatcatccgc tcgcgagctg
     1201 aagccgcctc tgcggccacc tcggccggga tgtcaccgat gtcgatcttg tcgcccaggt
     1261 cgctgccgta ggtgatggcc ttcatgttga tcaggtcgac cacgcctcgg aaggtgtcct
     1321 cggaaccgat gggcacctgc agcggcaccg cgttcgcgcc gaggcggtca tggatcatct
     1381 cgaccacgcg accgaagtcg gccccggtgc ggtccagctt gttcacgaag cagaagcgcg
     1441 ggacggtgta ccgatccgcc tggcgccaga cggtctccga ctgcggctcc acgccggcca
     1501 cgccgtcgaa caccacgacg gctccatcca gcacccgcag gctgcgctcg acctccaccg
     1561 tgaaatccac gtgcccgggc gtgtcaatca agttgatgcg atgatcggcc cagaagcagg
     1621 tggtggcggc ggcggtgatg gtgatccccc gctcctgctc ctgctccatc cagtccatgg
     1681 tggccgcgcc ctcgtggacc tcgccgatct tgtagatctt cttggtgtag aacaggatcc
     1741 gctcggacac cgtggtcttg cccgcgtcga tgtgggcgat gatcccgatg ttccgggtcc
     1801 gctccagcgg gatgttggtg gtcgccgagc gtccggcggc agtgctgacg gtcatgatcg
     1861 gcctaccact tgaagtggct gaaggcgcgg ttggcctccg ccatgcgatg ggtgtcctcg
     1921 cggcgcttca cggccgcgcc caggttgttg gaggcgtcca ggaactcggc cgacagcttc
     1981 tcggccatgc tccgtccgtt gcgcttgcgc gcagcctcga tcagccagcg catgccgagc
     2041 gacacgcgac ggtcggcgcg cacctcgacc gggatctggt aggtggaacc accgacgcgc
     2101 ttgggcttga cctcgatgag cggcatggcg ttgcgcaggg cgagctccat gacctccacg
     2161 ccggcgcgac ccgcctgctt ctcgcagcgg gcgatggcat cctccaggat gcgagaggcg
     2221 agctggcgct tgccgccgcg catcagcttg ttcgtgaact gatcgagcgt cagcgcggta
     2281 ccgaccaggt cgtcggcgta cttggtcttg cggggaacgg tgggacggcg gggcatcggt
     2341 ctagctgccc ttgccaggcg acttggcgcc gtacttcgag cgtccctgct tgcggtcgcg
     2401 cacgccggca gcgtccagcg tgccgcgcac gatgtggtac ttcaccgcgg gcaggtcctt
     2461 gacccggccg ccgcgaacca gcaccacgga gtgctcctgg aggttgtggc cgatgccggg
     2521 gatgtacgcg gtcacttcca tgccgttgac cagccggacg cgtgccacct tccgaagcgc
     2581 cgagttcggc ttcttcgggg tgcgggtcat gacctggatg cagacaccgc gacgctgagg
     2641 ggcgccgcgc ccctcggaag cggtctgctt cagggtgttc agcgtccggc gcagggccgg
     2701 cgccttgacc ttcttgcctg ccggcttgcg gcccttgcgg accagctggc tgatcgtcgg
     2761 caacgagaac ctcgcgttcg aatgacttgc tgtaggagtt ggaatcgctc gctcttcgag
     2821 tccctcgcga ggcacggcaa ccccgggagg ggggccacgt ctggcttcag cgaggggtgc
     2881 cggatgcggc acacacgaag ggcgccccgc aggcgggaca ccgaaggcgg agtatagggt
     2941 tcgggaccga tgagtgtcaa ccgagccatg ctcagcgcac cttgggcggg ccgatgcggt
     3001 acccgcaagg cttcatctcc ccgcagtggc gtccgggcgt gctctttaag catgcatcgt
     3061 ggggaaatgc ggtaccggcg cggccctacg gcgacgattc gcgccaggac gaactcccgg
     3121 cggccagttt cgaggcactt cctcccagtt aggcctcacc ggtacggcaa ttcgccacga
     3181 tctggggtcc aaggacagga ttcgtggaca tcctgcagta caggtagagc aacggctaca
     3241 ggttgcgagc accctccgag tcgaaggcgg cctgcacgat cagcggctcc tcgatcgcga
     3301 aggtgaccga tttcactaca ccgatgtcct ggcaggcgac cgccgcatcc gggcgcgttc
     3361 cctcgaggat gctcaccact agtggcccgt cttcgtccgg gcgctgggtg ctggcctcgg
     3421 ccaggccgca cccggtggtc ggctacc
//
LOCUS       DATACA010000011         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2249812, whole genome shotgun sequence.
ACCESSION   DATACA010000011 DATACA010000000
VERSION     DATACA010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249812"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..603)
                     /locus_tag="VHK28_00280"
     CDS             complement(61..603)
                     /locus_tag="VHK28_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2140694.1"
                     /translation="MAGGGGELKLAIPAPSLVLLVGPSGAGKSTFARRHFRETEVLSS
                     DRMRALVADDANDQRATAAAFELLHTTLALRLAGRRLSVVDATNVESWAREQLLAIAR
                     RHRRPAVAIVFALPLETCLERNLARRDRRLPAAAIRRQYRWLRDSQRAIPNEGFAGVW
                     VLESAEEVAAARVEIVPATA"
     gene            complement(579..1310)
                     /locus_tag="VHK28_00285"
     CDS             complement(579..1310)
                     /locus_tag="VHK28_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX2140695.1"
                     /translation="MCGRFAQPRSPEDLARIFRASPISDLPGDRYNVAPTDPVLGVTE
                     RHGERLLDEFRWGLLPTFATDRRAAARMINARMETVESSPAFRASFRSRRCLLPADAF
                     YEWIRHRDPATGRVRRSEPFAIRLREDEPMAFAGIWSVWRDPSSAERVFTAAIITGPP
                     NELVARVHDRMPVVLPPETWDTWLDESAPADQLRGLLRPAPADDFVIYPVSPSVNNVR
                     SDGPELLAPLTRWTEPEAWRVAGAS"
     gene            1670..>2685
                     /locus_tag="VHK28_00290"
     CDS             1670..>2685
                     /locus_tag="VHK28_00290"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX2140696.1"
                     /translation="MTRIGILTGGGDVPGLNSVIKSVVYRASELGWEALGIRRGWQGL
                     THLPPGETADAEWVRPLDRRSTRAIDRTGGTVLHTSRTNPARMPARALPAHLTDEQRR
                     ATAIDEGRHDLTPVVLDNIGRLGITHLVAIGGDDTLSYAARLGQDGVPLVAIPKTMDN
                     DVAGTEYCIGFSTAVTRAKELINRQRTTLGSHERIGVFRIFGRNAGFSAWYSAYVTST
                     RCVIPEAPFDLQLLADLLIEDRRLNASSYAMVIASEGAVYTGANLEEFGEADAYGHRR
                     KADIGFAIAEELKRRTGVDTVHSDLTYDLRSGDPDALDQMVATTFANIAVDMLMEGVT
                     GRMTA"
BASE COUNT          429 a          933 c          949 g          374 t
ORIGIN      
        1 ggcctgggcg ccggacatcc actacgtccg cgacgtggcg ctggccacca ccgcagagct
       61 ctaggcagtc gccggaacga tctcgacgcg ggccgcagca acttcctccg ccgactcgag
      121 gacccacacg ccggcgaatc cctcgttcgg aatggcgcgc tgggagtcgc gaagccatcg
      181 gtattggcgt cggatggccg ctgcgggcag gcgccgatcc cggcgggcca ggttgcgctc
      241 caggcacgtc tcgagcggca gggcgaagac gatggccacc gccgggcgtc ggtggcggcg
      301 ggcgatggcc agcagctgct cgcgtgccca ggattccacg ttggtggcgt cgaccacgct
      361 gaggcggcgc cccgccaggc ggagtgccag cgtggtatgc agcagctcga acgccgcagc
      421 ggtggctcgc tggtcgttgg catcatcggc caccagggcc cgcatgcgat ccgaggagag
      481 cacctcggtc tcgcggaagt gacgccgcgc gaacgtgctc ttgccggccc cgctgggtcc
      541 gaccagcagc accagggacg gggccggaat ggcgagcttc agctcgcccc cgccacccgc
      601 catgcctccg gctcggtcca gcgggtgagc ggtgccagca gctccgggcc gtcactgcgg
      661 acgttgttga ccgacggcga gaccgggtag atcacgaagt cgtcagccgg tgccggccgc
      721 agaaggccac gcagctgatc cgcaggcgcc gactcgtcga gccacgtgtc ccacgtctcg
      781 ggcggcagga cgaccggcat gcgatcgtgc acgcgggcca ccagctcgtt cggcggcccg
      841 gtgatgatgg ccgcggtgaa gacgcgctcc gcgctggacg gatcacgcca gacggaccag
      901 atcccggcga aggccatggg ctcgtcctcg cgcaggcgga tggcgaatgg ctcggagcgc
      961 cgcacgcgac cggtggccgg gtcccggtgc cgaatccact cgtaaaaggc atctgccggc
     1021 agcaggcaac gccgcgagcg gaacgaagcc cgaaaggcgg gagagctctc cacggtctcc
     1081 atccgcgcgt tgatcatgcg ggccgcggcg cgacgatcgg tggcgaaagt cggcagcagc
     1141 ccccagcgga actcgtccag caggcgctcg ccgtgccgct cggtcacgcc cagcaccgga
     1201 tcggtgggcg ccacgttgta gcggtcaccg ggcaggtcgc tgattggcga ggcacgaaag
     1261 atgcgcgcca ggtcctccgg ggagcggggc tgcgcaaagc ggccgcacat ggcgcgtcgc
     1321 tctcagcgcc gagctggggt caggcttcgg aagcggcgca ctccgcgcac attgctgccg
     1381 cagggatcgg gggctgtggg gagccggttg atcggggcag ggtctttccg cagactgtca
     1441 tttcggtatc acgctggacg tgaacctttg agaattggcg gtcgggcaag cgccaggtga
     1501 cgtagcgtcg ctgcttgagg tcggtggcca tgttcctcct cgggatggcc gcgatcggag
     1561 atcgcgggcg ggggacacac attatgcctc aattctcggt cgcccgccac ccgaccgggc
     1621 acgtcccggg tggatgcgcc cgtcgccgtg acgcggcatg atgaggcata tgacccgaat
     1681 cgggatcctg accggtggcg gtgacgtccc cgggctgaac tcggtcatca agagcgtggt
     1741 ctaccgtgcc agcgagctgg gctgggaggc actcggcatt cggcgcggct ggcagggcct
     1801 cacgcacctt ccgccgggcg agactgccga cgccgagtgg gtccgcccgc tcgaccggcg
     1861 ctcgacccgc gccatcgacc gcaccggcgg caccgtcctg cacacctcgc gcaccaaccc
     1921 cgcgcgcatg ccggctcggg cccttcccgc acacctgacc gatgaacagc ggcgtgccac
     1981 cgccatcgac gagggccgcc acgacctcac cccggtggtg ctggacaaca tcgggcgcct
     2041 gggcatcacg cacctggtgg ccatcggcgg ggacgacacg ctcagctacg ccgccaggct
     2101 gggccaggat ggcgtcccgc tggtcgccat ccccaagacg atggacaacg acgtggccgg
     2161 caccgagtac tgcatcggct tctcgaccgc ggtcacccgg gccaaggagc tgatcaaccg
     2221 gcagcgcacg acgctgggca gccacgagcg catcggtgtc ttccgcatct tcggccgcaa
     2281 cgcgggattc agcgcctggt actcggccta cgtgacgagc acccgctgcg tcatccccga
     2341 ggcaccgttc gaccttcagc tgctggccga cctgctgatc gaggatcggc ggctcaacgc
     2401 atccagctac gccatggtca tcgcctccga gggcgccgtc tacaccgggg ccaacctgga
     2461 ggagttcggc gaggcggatg cctacggcca cagacgcaag gcggacatcg gctttgcgat
     2521 cgcggaggag ctgaagcggc ggaccggcgt tgacaccgtc cactccgacc tgacctacga
     2581 cctgcgcagc ggcgatcccg atgcgctgga ccagatggtg gccaccacct tcgccaacat
     2641 cgcggtggac atgctgatgg agggcgtcac cgggcgcatg acggc
//
LOCUS       DATACA010000012         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2571229, whole genome shotgun sequence.
ACCESSION   DATACA010000012 DATACA010000000
VERSION     DATACA010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2571229"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VHK28_00295"
     CDS             <1..976
                     /locus_tag="VHK28_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX2140697.1"
                     /translation="LRVGDTVVLQRAGDVIPEVVSAVEDARDGSETVWEMPSQCPACG
                     TDAVREEGEVVWRCPNPWCPAQRIGGLLHFSGRGGLDVDGLGYKIMIQLVERNLVREP
                     ADVFRLDVETLEGLDRLGRKSAENLYGSIREARRRPLARIINALGIRHVGEQTAVDLA
                     AWLTSETPRDEGESEADWTRRAADRLRNASADELTAVFGIGRVVAESIARYFVDEHTA
                     DTLHRLIAAGVTAEAPEPGADPAAAVEGPLSGRTLVVTGTLPGFTRQEAEEAIRAAGG
                     HPAGSVSRATDFLVAGDKAGSKLAKAEQLGVPVLDEDGFRRLLESGNP"
     gene            973..1770
                     /locus_tag="VHK28_00300"
     CDS             973..1770
                     /locus_tag="VHK28_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX2140698.1"
                     /translation="MTSVLEQLASRMRTLHHGMEPLILPNAWDVPSARAVERAGAAAV
                     ATTSSGVAEALGYPDGEAIPAEEMLSAVERVAAAVRVPVTADMESGYGLDAADLVAGL
                     LQAGAVGLNLEDTDRTGLSPSLAAMETAAERIAAVREAARSAGVPVVINARVDVFLRG
                     IGRLEERVEEAIRRGRAYLEAGADCVYPIGLTDAAAIGRIVTALDAPVNVLLKPGAPP
                     ITELARLGVRRISVGGGLAQRAEAHVEELARGLLSGDGSAFATLNDR"
     gene            complement(1777..>1914)
                     /locus_tag="VHK28_00305"
     CDS             complement(1777..>1914)
                     /locus_tag="VHK28_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2140699.1"
                     /translation="LLDPADPGGWRIAFAVLAIGPALGILAMWRLRHRPDALRMAGGR
                     R"
BASE COUNT          264 a          658 c          740 g          252 t
ORIGIN      
        1 cctgcgcgtg ggcgacacgg tggtcctgca acgagccggc gacgtcattc ccgaggtcgt
       61 cagcgctgtg gaggacgccc gcgacggcag cgagacggtc tgggagatgc catcgcaatg
      121 cccagcctgc gggaccgatg ctgtgcgcga ggagggcgag gtcgtctggc gctgcccgaa
      181 cccctggtgc ccggcgcagc gcatcggtgg cctgctgcac ttctccgggc gcggcggcct
      241 ggacgtggac gggcttggct acaagatcat gatccagctg gtcgagcgca acctggtccg
      301 cgagccggcc gacgtcttcc gcctggacgt ggagacgctg gagggcctgg atcggctggg
      361 acgcaagagc gccgagaacc tgtacggctc gatccgggag gcgcgtcggc gtcccctggc
      421 gcggatcatc aacgcgctcg gcatccggca cgtcggcgag cagacggcgg tcgacctggc
      481 ggcctggctg acgagcgaga cgccgcggga cgagggggag tcggaggccg actggacccg
      541 ccgtgcggcc gatcgcctgc gcaacgcatc ggcggacgag ttgaccgcgg tgttcgggat
      601 cggccgggtg gtggccgagt cgatcgcccg ctacttcgtg gacgagcaca ccgctgacac
      661 gttgcaccgg ctgatcgcgg ccggcgtgac ggcagaggcc ccggagccgg gtgccgatcc
      721 cgctgccgcc gttgagggcc cgctgtccgg tcgcacgctg gtggtgaccg gcacgctgcc
      781 aggcttcacc cgccaggagg cggaggaggc catccgtgcc gccggcggac acccggcggg
      841 ctctgtctcc agggctaccg acttcctggt tgccggggac aaggctggca gcaagctggc
      901 caaggcggag cagcttggcg tgccggtcct cgacgaggac ggcttccggc gcctgctgga
      961 gagcggcaac ccgtgacatc ggtcctggag cagctcgcgt cgcgcatgcg caccctgcac
     1021 cacggcatgg agccgctgat cctgcccaac gcctgggatg tgccgagcgc gcgggcggtc
     1081 gaacgggccg gcgcggccgc ggtggcgacc acctccagcg gcgtggccga ggcgctgggg
     1141 taccccgatg gcgaggcgat cccggccgag gagatgctgt ccgccgtcga gcgcgtggcg
     1201 gctgccgtgc gggtcccggt gaccgccgac atggagtccg gctacgggct ggatgcggcg
     1261 gacctcgtgg ccgggctact ccaggccggc gccgtggggc tcaacctgga ggataccgac
     1321 cgcaccggcc tcagcccgtc gctggcggcc atggagacag cggccgagag aatcgccgcc
     1381 gtccgcgagg cggcgcggag cgccggggtg ccggtggtca tcaacgcgcg agtggacgtc
     1441 ttcctgcgcg gcataggcag gctcgaggag cgtgtggagg aggccatccg ccgcggccgg
     1501 gcctacctgg aggcgggcgc ggactgcgtc taccccatcg gattgaccga tgccgccgcc
     1561 atcggtcgta tcgtcacggc cctggatgcc ccggtcaacg tgctgctcaa gcctggcgct
     1621 cccccgatca cggagctggc ccggctgggc gtgcggcgca tcagcgtggg cggcggtctc
     1681 gcacagcggg ccgaggcgca cgtcgaggag cttgcccgtg ggctgctgtc cggcgacggc
     1741 tcggcgttcg ccaccctgaa cgaccgctag ccgacctcag cgcctgccgc ccgccatgcg
     1801 cagcgcatcg ggccggtgac gcagccgcca catggccagg atgccgagcg cgggaccgat
     1861 ggccagcacg gcgaaggcga tccgccaacc gccgggatcg gccggatcca gcag
//
LOCUS       DATACA010000013         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2410622, whole genome shotgun sequence.
ACCESSION   DATACA010000013 DATACA010000000
VERSION     DATACA010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410622"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VHK28_00310"
     CDS             complement(<1..308)
                     /locus_tag="VHK28_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140700.1"
                     /translation="MTAMFVGFSWSVLVSGSGATSLFVAAVAFAVAGIIVFTGDVSAL
                     RAGTATLAGHVAAMAVAGPVVLLVNPLTRTVLPGTFTFVVAGIVIASLLCGGRLNLAV
                     V"
     gene            535..1314
                     /locus_tag="VHK28_00315"
     CDS             535..1314
                     /locus_tag="VHK28_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2140701.1"
                     /translation="MDEVFKALSDPGRRALLDALHERDGRSLGELEEVLPGMTRFGVM
                     KHLRVLTAAGLVTVRRDGRRKLHYLNPVPVQLIADRWISRYAEPWTTGMADLKATLEA
                     TAPMTAPKHVFQVYIRTTPERLWQALTDPAWTQRYYFNSLFESDLQPGSPFTYRQPEG
                     RLDIDGQIVEADPPRRLVHTFNIRWDPENSDPPSRVTWEITPMGETCQLTLVHDGFET
                     ETATFGETGSGWPLILSNLKTLLETGEPLDIGEPETAEATA"
     gene            complement(1335..2117)
                     /locus_tag="VHK28_00320"
     CDS             complement(1335..2117)
                     /locus_tag="VHK28_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HEX2140702.1"
                     /translation="MTQLIALAGFGFLLGMRHATDADHVIAVTTILNRSRRFLDGTVI
                     GAIWGLGHTITVLVVGMLIIVFNVVIPPPVGLAMEFAVAVMLMALGILNLTGVMRSLT
                     ERFTPPAPLHGHDHAHLHAHDGADTHGYEHAHLHGHGVQRGLVATFGWFHLLRPLAVG
                     LVHGLAGSAAVALLVLATIQDAGQAIVYLLIFCAGVAAGMAILTTVIGLPFLVSRARS
                     ERINRWLTVGSGVLSLAFGLYLAYQIGFVDGLFTGEFNWEPA"
BASE COUNT          406 a          727 c          714 g          298 t
ORIGIN      
        1 acgaccgcaa ggttgagccg ccctccacag aggagcgagg cgatgacgat gccggcaacg
       61 acgaaggtaa aggtgcctgg cagaacggtg cgtgtgagcg ggttcaccag caggacaacc
      121 ggaccagcta ccgccatcgc ggccacgtgg cccgcgagcg tcgccgttcc cgcgcgcaga
      181 gccgacacgt cgcccgtgaa cacgatgatc cccgcaaccg cgaaggccac tgccgcaaca
      241 aacagcgagg tagcaccgga cccggagacg agcacgctcc aactgaagcc tacgaacatg
      301 gcggtcaccg cgccggccgc ggcaacgacg ccaccgagta ctccgcgaag gagctgggtc
      361 ttcatgctcg gcatagagtg cgactcctcg tttgcccgtc ccgctcgcgg caacaccatc
      421 tggggccgta acagtaccgg cgtcctcagt gtcgccgtca acgctcgttc atgacgctcc
      481 ggcgccgcat gcgccttctt gacatgtgac cagtaagtca cctatagtcc ccgggtggac
      541 gaggtgttca aggcgctctc cgaccccggt cgacgcgctc tgctcgacgc cctgcacgag
      601 cgggacggcc gctcgctcgg cgagctcgag gaggtgctgc cggggatgac ccgcttcggg
      661 gtgatgaagc acctccgggt gctgaccgcc gcggggctgg tcacggtgcg ccgcgatgga
      721 cgccgcaagc tccactacct caacccggtt cccgtccagc tcatcgcgga ccgatggatc
      781 agccgctacg ccgagccgtg gacgaccggg atggccgatt tgaaggcaac cctggaggca
      841 acagcaccca tgaccgcacc caagcacgtg ttccaggtgt acatccgcac caccccggag
      901 cggctgtggc aagcgctcac cgatccggcc tggacccagc gctactactt caactcgctc
      961 ttcgagtccg acctgcagcc gggctcgcca ttcacctacc ggcagccgga gggccggctg
     1021 gacatcgacg gccagatcgt cgaggcggac ccgccccggc ggctggtcca caccttcaac
     1081 atccgatggg atccggagaa cagcgatccg ccgtcacgtg tcacctggga gatcacgccg
     1141 atgggcgaga cctgccagct gaccctggtt cacgatgggt tcgagaccga gacggcgacc
     1201 ttcggcgaga ccggcagcgg atggccgctg atcctctcca acctgaagac gctgctcgag
     1261 accggcgagc cgctggatat cggcgaaccc gagacggcgg aggcgaccgc ctgaccgatt
     1321 gggagtcggc gtctctacgc cggctcccag ttgaactcgc cggtgaagag gccatccacg
     1381 aacccgatct ggtaggccag gtagaggccg aacgccaggg acagaacgcc cgagccgact
     1441 gtcagccagc ggttgatgcg ctccgagcgc gctcgggaga cgaggaacgg caaaccgatc
     1501 acggttgtca ggatggccat gccggcggct accccggcgc agaagatcag caggtacacg
     1561 atggcctggc ccgcgtcctg gattgtggcc aggaccagca gggcgaccgc ggcggaccca
     1621 gccaggccgt ggaccagccc caccgccagt ggtcgcagca gatggaacca gccgaaggtg
     1681 gccaccaggc cgcgctggac accgtgcccg tgcaggtgcg cgtgctcata gccgtgggta
     1741 tcggcaccgt cgtgggcatg caggtgcgcg tggtcgtgcc cgtgcagcgg cgcgggcggc
     1801 gtgaagcgct cggtgagcga acgcatcacg ccagtgaggt tgaggatgcc cagggccatc
     1861 agcatcaccg ccacggcgaa ctccatggcg agcccgaccg gcggtgggat caccacgttg
     1921 aagacgatga tcagcatccc gaccaccagg acggtgatcg tgtgacccag gccccagatc
     1981 gccccgatca cggtgccgtc caggaagcgc cggctccggt tgaggatggt ggtcacggca
     2041 atcacgtgat cggcatcggt tgcgtggcgc ataccgagca ggaagccgaa gccggccagg
     2101 gcaatgagct gggtcatggt ggtggattct agcggccgtc acctg
//
LOCUS       DATACA010000014         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1125475, whole genome shotgun sequence.
ACCESSION   DATACA010000014 DATACA010000000
VERSION     DATACA010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125475"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VHK28_00325"
     CDS             complement(<1..450)
                     /locus_tag="VHK28_00325"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX2140703.1"
                     /translation="MSWQLDASHSSVNVSAKHMMVTTVRGTLAIRSAVLDFDPERPEA
                     GRVEAVIDAASINTGVEQRDQHLRSADFLDVEDHPDITFRSTRITPAGDGYRVHGGLT
                     IRGVTRPVVLDAEVTGVVDDWQGGRRAAFSASTRINREDWGLTWNVAL"
     gene            572..1045
                     /locus_tag="VHK28_00330"
     CDS             572..1045
                     /locus_tag="VHK28_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2140704.1"
                     /translation="MKTQPTTTPSRDDPRLSAWRTFLYAHAQVRRQLERELQAEQAMG
                     LGDYEVLLILAYSPERRKRMSELADALVLSRSGATRLVDRLEAAGLVERVSCETDRRG
                     QWAQLTEAGYERLRGAAPTHLRGIAEHFLDRIPAGELADLERTLGRVLTEPIGLP"
     gene            complement(1042..>1941)
                     /locus_tag="VHK28_00335"
     CDS             complement(1042..>1941)
                     /locus_tag="VHK28_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140705.1"
                     /translation="SWRRSMGELLLLLLVAGGVALAVAWPLLDGGGRPPMEGGTPEDE
                     ALLVRHRLAIEALRDVEADRRAGSLDDAAYAAQREEAERHAAATLAALEGTQPGDGAV
                     SSPRSGGGRLAAGVAAVLAALLLAGFALPAPLGLAERTETDTALAEAMAREEGRQARI
                     GALLERLAADANDPEALSALADAYLAGSTAEDRRRGAVALLALISLEPENASAYRRLI
                     TAYVNAGDWTDAEAATDSFAEVVGEQDPDVPFFRGLIALRGENDGAEALRQFDRFLEL
                     APDDPRAGMVRGLRAEALGEPEG"
BASE COUNT          278 a          711 c          693 g          259 t
ORIGIN      
        1 cagggccacg ttccaggtca ggccccagtc ctcgcggttg atgcgggtcg acgcgctgaa
       61 ggctgcccgc cggccgccct gccagtcgtc gaccacgccg gtcacctcgg catccaggac
      121 aacgggtcgg gtgacgcccc ggatggtgag gccgccatgg acgcgatagc cgtcgccggc
      181 cggggtgatg cgggtgctcc ggaaggtgat gtccggatgg tcctcgacgt ccaggaagtc
      241 cgcggagcgc aggtgctggt cacgctgctc cacgcccgtg ttgatggagg cggcgtcgat
      301 gacggcctcc acccgaccgg cttctgggcg ttccgggtcg aagtcgagaa cggcggatcg
      361 gatggcgagc gtgccgcgca cggtggtcac catcatgtgc ttggccgata cgttgaccga
      421 gctgtgagag gcgtcgagtt gccaggacat gggatagggt tctcctgctg cgcgccgcga
      481 tccgtaccgg gacccggcgg aagtgattgc gcaagcaacg atatcaccaa ctacttgcgg
      541 ggtcaaccat ttcctgccag catgtgaggc gatgaagacg cagcccacga ccacccccag
      601 ccgcgacgac ccgcgcctga gcgcgtggcg gaccttcctg tacgcccacg cgcaggtgcg
      661 acgccagctc gagcgcgagc tgcaggcgga gcaggccatg ggcctcgggg attacgaggt
      721 gctgctgatc ctggcctact cgcccgagcg caggaagcgc atgagcgagc tggccgacgc
      781 gctcgtcctg tctcgatccg gagccacacg gctggtcgac cggctggagg cagccggcct
      841 ggtggagcgg gtcagctgcg agacggatcg ccgtgggcag tgggcgcagc tcaccgaagc
      901 cggctacgag cgcctgcgcg gggcagcgcc cacgcacctg cgcggcatcg cggagcactt
      961 cctggatcgc atcccggccg gcgagctggc cgacctggag cgcaccctgg gccgcgtcct
     1021 gaccgagccg atcggcctgc cctagccttc cggctctcca agggcctcgg cacgcaggcc
     1081 ccgcaccatc ccggcacgag ggtcgtccgg cgccagctcc aggaaccgat cgaactgccg
     1141 cagcgcctcg gcgccgtcgt tctcgccgcg cagggcgatc aggccacgga agaacggaac
     1201 gtcggggtcc tgctctccga ccacctcggc gaacgagtcg gtggccgcct cggcatcggt
     1261 ccagtcgccg gcgttgacgt aggccgtgat gagccgccga taggcagatg cgttctccgg
     1321 ctcgaggctg atgagggcca gcagggcgac cgcaccccgg cggcggtcct cggcggtact
     1381 gcctgccagg tacgcgtccg ccagcgcgga aagtgcctcg gggtcgttcg cgtcggccgc
     1441 cagccgctcc agaagtgccc cgatgcgagc ctgccgcccc tcctcgcggg ccatggcctc
     1501 cgccagggcg gtgtcggtct cggtccgctc cgccagtccc agcggcgccg gcagcgcgaa
     1561 gccggccagc agcagcgcgg ccaggacggc agcgacccct gccgccagcc gtccgccgcc
     1621 ggagcgcggt gagctcaccg ccccatcccc gggctgggtg ccctccagcg cggccagcgt
     1681 ggccgccgcg tgacgctcgg cctcctcgcg ctgggccgcg taggcggcat cgtcgagcga
     1741 gccggcacgg cgatccgcct ccacgtctcg cagggcctcg atcgccaggc ggtggcggac
     1801 cagcagcgcc tcgtcctccg gcgtgccgcc ctccatcggg ggccggccac cgccgtcgag
     1861 cagcggccag gcgacggcca gcgcgacgcc gccggccacc agcaagagga ggagcagctc
     1921 acccatcgag cgcctccagc t
//
LOCUS       DATACA010000015         3952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1848518, whole genome shotgun sequence.
ACCESSION   DATACA010000015 DATACA010000000
VERSION     DATACA010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848518"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..967)
                     /gene="tmk"
                     /locus_tag="VHK28_00340"
     CDS             complement(329..967)
                     /gene="tmk"
                     /locus_tag="VHK28_00340"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019621826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX2140706.1"
                     /translation="MTAAPQRGRFITLEGSEGSGKTTAARHLAEWLRSRGERVVLTAE
                     PGGTPLGEEVRRLVLHQRDLSDDLDPRADALLYAAARAQHTARVILPALERGEHVVCA
                     RYLDSSLAYQGVGYGNDPDEMRRLQVFATYDTLPDLTILLDVPVDVGLGRKRRGEWNR
                     FEDSLSVDFFEKVRRAYLDLAVAEPDRFRVVDAAGSVAETDQLVREVVEPIL"
     gene            complement(1017..2858)
                     /locus_tag="VHK28_00345"
     CDS             complement(1017..2858)
                     /locus_tag="VHK28_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX2140707.1"
                     /translation="MEAERRVTRQETTDDLDALMAALPDEIVERLRGVANRSDLLEVV
                     MDLGRRPEARFTHGEEVLLDREINEADIAHVIDHIGAFGDDNRAGIERTLHRISAIRN
                     RAGKVVGLTCRIGRAVFGTIDIIRDIAESGQSILILGRPGVGKTTMLREVARVLADDL
                     GKRVIVVDTSNEIAGDGDIPHPGIGDARRMQVRTPTDQHGVMIEAVENHMPEVIVIDE
                     IGTELEAAAARTIAERGVQLVGTAHGNSLDNLMLNPTLSDLVGGIQPVTLGDEEARRR
                     GTQKTVLERKAPPTFDVLVEIVERDNVIVHRNVAETVDAILRGHMVPPEARWRDDGGE
                     LKAVTKYDYRISETAAGNPAFAPLEPTGGFGAFSGGGGGGRGRGSLSASGLRPLPGRS
                     AGRGGRTLPGERLSGDRFESTDASDPRRLMEQALRDSAVSSAPTDDERLDRPVGANRP
                     ASAAARSHRPMSLFAFGVSRKRLEQAVREMGVPVTSARDLEEADAVVTLRNYYRRKPS
                     ALREAESNGIPIYVLKTNTMQQMENLLASLFDLETDPSEAALRETSEAIGLVQASGRP
                     VELLPQNAYVRRLQHQMAERNNLMSRSRGAEPNRRVELIPDEGRAWR"
     gene            complement(2866..>3952)
                     /locus_tag="VHK28_00350"
     CDS             complement(2866..>3952)
                     /locus_tag="VHK28_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140708.1"
                     /translation="SAPAGAGGRVTVGGAIRPARLSDRAALGELSRRVHLSPDSYRRS
                     LGVPAPSATLPRISLSTFIPSWLPLRHPSVHLVAEADGELVGSCRAIEEPGRHDWVIT
                     ELDASDGPMAGEVRYALLRALVDEAIQHDVERFHAACSDVRENLELFGQLSFMAYAQE
                     EILYRPPETLRGPRSWVRSLLRRGDRQDGTERPAPDPALDLRPAGAPDAWHLFDLWAH
                     ATPPAIARIEGYAAGDWEDVAHEGVVPRSSLNPLLHFSGMDAWLLPHEQRAGGFAQHG
                     SCREGPHYLRYLVRDGTDGASFLRAVLDAMGPDAVGAGILSPVRTYEAAGLRAAAAVG
                     FEPVGRVTLLVREVRAAVRQPAMVPAI"
BASE COUNT          521 a         1434 c         1406 g          591 t
ORIGIN      
        1 taccggtcgg tcgttgcatg ctggcctcgt catattgctg gcacggcgga atggaagggc
       61 gggccgtgcc gcggcgcgag tgtacgctgg ggcggagccc tggggcccgc tcggcctgcc
      121 agggcgatgt ccgctcggcc cgggtgatcg ccgctgccgc cagctgacga agcctccgat
      181 tcacatcagg cgcgcaccac ggggcgccgc tgcccgtcgg gggggtccgg attcacaacg
      241 ggcgtgaatt cggaatcttc ataggcccgt tccggcactc catggtcgcc tggtgtgaat
      301 ccgcgccgtt cgcaaggtcg gagagccgct agaggatcgg ctccaccacc tcgcggacca
      361 gctgatctgt ctcggcgacg ctgcccgccg cgtccaccac ccggaagcga tccggctcgg
      421 cgaccgcgag gtcgaggtac gcgcggcgca ccttctcgaa gaagtcgacc gacaggctgt
      481 cctcgaacct gttccactcc ccgcggcgct tgcggcccag gccgacgtcc accggcacgt
      541 ccagcaggat ggtgaggtcc ggcagcgtgt cgtaggtggc gaacacctgg aggcggcgca
      601 tctcgtccgg gtcgttgccg taccccacgc cctggtaggc cagcgacgag tccaggtagc
      661 gcgcgcagac cacgtgctcg ccgcgttcca gggctggcag gatgactcgg gccgtgtgct
      721 gcgcccgggc ggcggcgtag aggagcgcat cggcgcgcgg gtcaaggtcg tccgacaggt
      781 cgcgctggtg gagtaccagc cgtcgcacct cctcgcccag cggtgtgccg cccggctcgg
      841 cggtgagcac gacgcgctct ccacgcgatc gcagccactc ggccaggtgg cgtgcggcgg
      901 tcgtcttgcc gctgccttcg gacccttcaa gcgtgatgaa gcggccgcgt tggggcgcgg
      961 ccgtcatcgg tccaccgcgg gctgcaaacg gggctgtagg cggggcagca actggctcag
     1021 cgccaggccc gaccctcgtc ggggatgagc tccacccggc ggttcggctc ggccccgcgg
     1081 gatcgcgaca tgaggttgtt gcgctccgcc atctggtgct ggaggcgcct gacgtatgcg
     1141 ttctgcggca gcagctcgac gggcctgccg gatgcctgga ccaggccgat ggcctccgac
     1201 gtctcgcgca gtgccgcctc ggagggatcg gtctccaggt cgaagaggct ggccagcaaa
     1261 ttctccatct gctgcatggt gttcgtcttc agcacgtaga tggggatgcc attcgactcc
     1321 gcctcgcgca gcgcggacgg cttgcgccga tagtagttgc gcagggtgac cacggcgtcc
     1381 gcctcctcca ggtcacgggc gctggtcacc ggcacgccca tctctcgcac cgcctgctcc
     1441 agccgcttgc ggctgacccc gaaggcgaac aggctcatgg gccgatggct gcgcgcggcg
     1501 gccgatgccg gccggttggc cccgaccggg cggtccagcc gctcgtcgtc ggtgggcgcg
     1561 gagctcacgg cgctgtcgcg cagcgcctgc tccatcaggc gccgcggatc cgaggcatcg
     1621 gtggactcga agcggtcgcc ggagagccgc tcgccgggca acgtgcggcc cccgcgaccg
     1681 gccgagcgcc cgggcagcgg gcgcaggccg ctggcggaca gcgatccacg gccgcggcca
     1741 ccccctccac caccgctgaa ggcgccgaag ccgccggtcg gctccagggg ggcgaaggcg
     1801 gggttgccgg cggccgtctc gctgatgcgg tagtcgtact tggtcaccgc cttgagctcg
     1861 ccgccgtcgt cgcgccagcg cgcctcgggc ggcaccatgt ggccgcgcag gatggcgtcc
     1921 accgtctcgg ccacgttgcg gtggacgatg acgttgtcgc gctccacgat ttcgaccagc
     1981 acgtcgaacg tcggcggcgc cttgcgctcc agcaccgtct tctgcgtccc gcggcggcgc
     2041 gcctcctcat cgcccagggt caccggctgg atgccaccca ccaggtccga cagggtcggg
     2101 ttgagcatca ggttgtccag cgagttgccg tgggcggtac ccaccagctg cacgccgcgc
     2161 tccgcgatgg tcctggcggc cgcggcctcc agctccgtcc cgatctcgtc gatgacgatc
     2221 acctcgggca tgtggttttc gaccgcctcg atcatcaccc cgtgctggtc ggtgggggtg
     2281 cgcacctgca tgcggcgcgc gtcgccgatg cccggatgcg ggatgtcccc gtcgccggca
     2341 atctcgttgc tggtgtcgac cacgatcacc cgcttgccca ggtcgtcggc cagcacgcgg
     2401 gccacctcgc gcagcatggt cgtcttgccc acgccgggcc gtcccaggat caggatcgac
     2461 tgccccgact cggcgatgtc gcggatgatg tcgatggtgc cgaataccgc ccggccgatg
     2521 cggcaggtca ggcccaccac cttgccggcg cggttgcgga tggcgctgat gcggtgcagg
     2581 gtgcgctcga tgccggcgcg gttgtcgtcg ccgaaggcac cgatgtggtc gatcacgtgg
     2641 gcgatgtcgg cctcgttgat ctcccggtcg agcagcacct cctcgccgtg cgtgaagcgc
     2701 gcctccggcc gccgcccgag gtccatcacc acctccagca ggtcggaacg gttggcaacg
     2761 ccgcgcagcc gctcgacgat ctcgtcgggc agcgccgcca tcagggcgtc aaggtcgtcc
     2821 gtcgtttcct gccgcgtcac gcgtcgctcg gcctccatcg ccatctcaaa tcgccggcac
     2881 catggccggc tggcgtaccg ccgcgcgcac ctcccggacc aggagggtca ctcgcccgac
     2941 cggctcgaag ccgaccgcgg ctgccgcgcg cagccccgcc gcctcgtatg ttcgcaccgg
     3001 cgacaggatt cctgcaccta ccgcgtcggg ccccatcgca tccaggaccg cacgcaagaa
     3061 cgatgccccg tccgtcccgt cgcggaccag gtagcgcagg tagtgcggac cctcccggca
     3121 gctgccgtgc tgcgcgaagc cgccggctcg ctgctcgtgc ggcagcagcc aggcgtccat
     3181 gccggagaag tggagcagcg ggttgaggct gctgcggggc accacgccct cgtgcgccac
     3241 gtcctcccag tccccggcgg catagccctc gatgcgggcg atggccggtg gcgtggcgtg
     3301 cgcccacagg tcgaacaggt gccaggcgtc gggggcgccg gccggccgca ggtcgagcgc
     3361 cggatcgggg gcgggccgct ccgtgccgtc ctgccgatcg ccgcggcgca gcagcgaccg
     3421 cacccaggag cgcgggcctc ggagcgtctc gggagggcgg tacaggatct cctcctgcgc
     3481 gtaggccatg aagctcagct gcccgaagag ctccaggttc tcgcgcacgt ccgagcaggc
     3541 ggcgtggaag cgctccacgt cgtgctggat ggcctcatcc accagcgcgc gcagcagcgc
     3601 gtagcggacc tcgccggcca tcggtccatc cgaggcgtcc agctcggtga tgacccagtc
     3661 gtggcggccg ggctcctcga tggcccggca ggagcccacc agctcgccat ccgcctcggc
     3721 caccaggtgg accgatgggt ggcgcagcgg cagccaggac gggataaagg tggataggct
     3781 gatgcgcggc agggtggccg atggcgccgg cacccccagt gagcgccggt agctgtccgg
     3841 gctgaggtgg acgcgccggg acagctcccc cagcgcggcc cggtcactga gccgcgcggg
     3901 ccggatggcg ccgccgaccg tcactcgccc accagctcca gcaggagccg at
//
LOCUS       DATACA010000016         8752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_402890, whole genome shotgun sequence.
ACCESSION   DATACA010000016 DATACA010000000
VERSION     DATACA010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402890"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VHK28_00355"
     CDS             complement(<1..249)
                     /locus_tag="VHK28_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140709.1"
                     /translation="MDGYSINDAATVLGVPEARVWELIARGVLAGSPEADGGMRVFLK
                     PSEAPAHVSSRDTDAPDAAAAGGRRQNGNGGSHVESHPE"
     gene            complement(301..1581)
                     /locus_tag="VHK28_00360"
     CDS             complement(301..1581)
                     /locus_tag="VHK28_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEX2140710.1"
                     /translation="MTTQVERTGLDVDAVRDHFPALSRTRDGLPVAFLDGPGGTQVPR
                     ACIDGMVRYLESSNANQGGAFDASVETDALLAEAHAATADFLGAHHSGEVIFGPNMTT
                     LTFALSRAVGRELGEGDEVVVTRLDHDANVAPWLAMAEDRGATVRWIDVRPEDCTLDL
                     GGLESLIGERTRLVAFGLASNAVGTVNDAGRLIEMAHAYGAWAFVDAVHAAPHLPIDV
                     APLRADFLACSPYKFFGPHLGVLYGRADLLDRLGAYRVRPAGDGLPGKWEVGTQSHEA
                     LAGLLGTYDYLASLGRAYGGGSRDDGRRGALRAAMSAIRGYERTLIGPFLEGLAAVRG
                     LRVYGITGAARVDERVPTVAFTLEGRQPRQVAAHLAERGISVWDGDYYAVELVRGLGL
                     DRSGGMVRVGLVHYNTLGEVERLVAALEEIAPPS"
     gene            1651..3831
                     /gene="glgP"
                     /locus_tag="VHK28_00365"
     CDS             1651..3831
                     /gene="glgP"
                     /locus_tag="VHK28_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX2140711.1"
                     /translation="MTTSLRVPTLPGYDFNLPRGLEGLGDLAYNLWWSWTPRAGSVFA
                     RMDAQAWGRHRNPIPVLRAMDSNRWAELTADEDFMVDVSRLLDEYERYMGNGADTWYG
                     AGVATGTIPELPGTIAYFCAEYGFHESMQIYSGGLGVLAGDHLKSASDAALPFVGIGL
                     LYRRGYFRQQIDADGHQEHAQPDLDPGQLPLRRARGPNGGPVQVSVDFPGRQVHAAVW
                     VAQIGRVPLLLLDTDIPANEEPDRPITHILYVRGREMRLCQELVLGVGGVRALRRLEI
                     EPAVWHLNEGHSAFLLLERARELLAESPGLAPTEALRRVGGDSVFTIHTPVPAGNEVF
                     ERGLMAKKLEPWLAETGMPAGELMELGRGRTDDPEAGFDMTAFVLRHAADANAVSRLH
                     AETATDTWQEVAGHPIRAITNGVHVATWLGRPIRRLSERAIGTSLASDVADEGTFAGL
                     DGLDGSDLWAAHLQQKRELIASTERRLVSQFARHGEAPDDLREIDHALDPNALIIGFA
                     RRFATYKRADLIFRDEDRLAALLANPERPVQIVIAGKAHPADRPGQQVIQRIFQLTRS
                     DRLHGRVFILEDYDMRIARFLVGGVDIWLNNPRRPMEASGTSGMKAALNGIPSVSILD
                     GWWDEGYNGSNGWAIGGRGYETDDPGAQDAQDAEDLYRLLEQEIVPCYFDRNEDGVPQ
                     GWIRLMRAAIGSSIWQFSTRRMLGEYVEQLYLPNVASRREAVPT"
     gene            3874..4611
                     /locus_tag="VHK28_00370"
     CDS             3874..4611
                     /locus_tag="VHK28_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140712.1"
                     /translation="MTTTEVTQGNTMTPLHADLLALLHAMRRAERSIFAGLSPSVRDA
                     PGTIGAWSAKDAQAHLAAWRSIEARRLEATVAGGELPSSDPAPDAPVDEANAHIHEQR
                     AGLGWDAVEREADDSVAALAAAIERSSFDALCVCEGTTASIGANGVNHAIAHLSDVAR
                     LGASKADFDEFAREVEAILGRGHLPVRDSGVMLYNLACHAALSGDLEDARRLLADAIE
                     RRHDLAEVAREDPDLAALGPQARGVTA"
     gene            complement(4608..5993)
                     /locus_tag="VHK28_00375"
     CDS             complement(4608..5993)
                     /locus_tag="VHK28_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX2140713.1"
                     /translation="MRRRARLAVTLVALLMVGGTLLTDSHRDLALAADPLADAKAQRA
                     SIERQLSDQRTRLSELKSRSASLADQLDVAEAELAEVSAEYERVVALLAQVREQVAEI
                     RARLTELLAQIAEMDDELAAVAAEVAHQTDALHAREALLQDHLRSAYEQSQTSLLEVI
                     LSSESLDAATNQVGYLLTLSEQDRQLADEIGVIREQLEVKEDTLREGRRALRTARLAA
                     SEEEALLAERQAELTKLEARTAELKAAAEKKRAEQEAALNAALDAQQDVSAQIAANEQ
                     AFAAANALVQRVAAEQAALEEARRREAEAARKRAQQQQRPPNVSASGFRWPEASTRIT
                     QEWGPTSFALEPPYTYHGTYYPHFHGGIDMANGCGTPILASKAGVVVASGQPLWPYDT
                     GYGVIIDHGGGIQTWYWHITPRVIVSPGQVVTTGQTIGYEGTTGFSTGCHLHFATNIH
                     GVWENPRNILP"
     gene            complement(6180..7229)
                     /locus_tag="VHK28_00380"
     CDS             complement(6180..7229)
                     /locus_tag="VHK28_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX2140714.1"
                     /translation="MIDGIKRRLKAADAWLIGHRWTRIGRRALLGFLRHEALQNAGSM
                     AYFSILSLFQLLVLAVVVLSFFVGQGEARQFVVERIEQATPVDAATMGEVIDSIIETR
                     GGVTIFGLIFLIWGALGIFSAVSKGISAAFVSAEPRPFLQDKLIGVALMSMTGLLGIA
                     SVAIGVAAGILQNAAAEVLDQVPGGGVAVAAIGFLAPLVLIFFAFLALYRLVPNRPVS
                     IAEIWPGAVVATVLWTALRIGFTYYATDVANYDSAFGPISAAISLLVFLYFASIVVLL
                     GAEVARANVVDDEVMEGERALGGERPPSRPPVPVPVAGSAGARGRKMPGWSLAVGGVV
                     AAGVVRFLSGRRRGR"
     gene            7282..8682
                     /locus_tag="VHK28_00385"
     CDS             7282..8682
                     /locus_tag="VHK28_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX2140715.1"
                     /translation="MPKDASVQLELDGFDVTVSNPGKVFFPDAGITKIDLVNYYLAVA
                     DGALRGVMDRPMALKRFVDGISAEAFFQKRAPKSVPEFVRTVELSFPSGRTADEVVVS
                     NRAALVWVVNLGCVDLNPHPVRADDLAQPDELRVDLDPGPGVAWDDVRRVAMVVREVL
                     SDHGLTGWPKTSGSRGIHVYARIERRWGFSDVRRAALALAREVERSAPEISTSKWWKE
                     ERLGVFIDYKQNAKDRTVASAYSVRPVVSAQVSAPLTWDEVPHAELGDFTLRSMPDRY
                     ARLGDVSAGIDSAVGSLEGLLELSARDEASGLGDAPWPPHYTKAEGEPPRVQPSKRRR
                     QEPRPPTDPSAPRGRAGSVAPATVSGSYRRADGTVSPTGRRRSRHPLLVVAQAKAKAD
                     ALAGLDRWKARHPEAAVHLAEDDVLVDGMRGRSSLWYRVRVNLRHVPENARPDQEPPD
                     PYYDPADEWLGAESRD"
BASE COUNT         1306 a         3148 c         3035 g         1263 t
ORIGIN      
        1 ctccggatgc gactcgacgt ggctgccgcc gttgccgttc tgccgccgcc cgccggctgc
       61 ggccgcatcg ggggcgtcgg tatcccgcga gctcacgtgg gccggtgcct ctgacggctt
      121 gaggaagacg cgcatgccac catcggcctc cggagacccg gccagcacgc ctctggcgat
      181 cagctcccag acgcgtgcct cggggactcc gaggaccgtc gccgcatcgt tgattgagta
      241 gccgtccatc gggggcgata gcgtaccgct cctgtcgagc ggcctccacg cgccactcgg
      301 tcagctgggc ggagcgatct cctcaagcgc ggccaccagc cgctcgacct cgcccagcgt
      361 gttgtagtgt acgaggccca cgcgcaccat gccgccggat cggtccagcc cgagcccccg
      421 caccagctcc acggcgtagt agtctccatc ccagaccgat atcccccgct ccgcgaggtg
      481 cgctgcaacc tgccggggtt gccttccctc cagggtgaag gcgacggtcg gcacccgctc
      541 gtcgacgcgt gccgcgcctg tgatgccata gacccgcagc ccgcggactg ccgcgagacc
      601 ctctaggaat ggaccgatga gcgtgcgctc gtacccgcga atggcggaca tggccgcccg
      661 cagggcgccg cgcctgccgt cgtcgcgcga gccgccaccg taggcacggc ccaacgaggc
      721 caggtagtcg tacgtcccga gcaggccggc cagcgcctcg tggctctggg tgccaacctc
      781 ccactttccg ggcagaccat cgccggccgg ccgcacgcga taggcgccca gccggtcgag
      841 gagatcggcg cggccgtaca gcacgccgag atgcgggccg aagaacttgt acggcgagca
      901 ggccaggaag tcggcccgca gcggggccac gtcgatcggc aaatggggcg ctgcgtggac
      961 ggcatccacg aaggcccagg caccgtaggc gtgcgccatc tcgatcaggc gcccggcgtc
     1021 gttcaccgtg ccgaccgcgt tggaggccag tccgaatgcc acgagccgcg tccgctcacc
     1081 gatgagcgat tcgagcccgc ccaggtccag cgtgcagtcc tcggggcgaa cgtcgatcca
     1141 gcgcactgtg gctccccggt cctcggccat ggccagccag ggcgccacgt tggcgtcgtg
     1201 atcgaggcgg gtgaccacca cctcgtcacc ctcacccagc tcccggccga cggcccggct
     1261 cagcgcgaag gtcagcgtag tcatgttcgg cccgaagatg acctcgcccg aatggtgggc
     1321 acccaggaaa tcggccgtgg cggcgtgcgc ctcggccagg agcgcatcgg tctcgaccga
     1381 tgcgtcgaag gcaccaccct ggttggcgtt gctcgactcg aggtagcgga ccatgccgtc
     1441 gatacaggcg cgcgggacct gcgtcccgcc cggcccatcc aggaaggcca ccgggagacc
     1501 gtcgcgagtg cgtgacagcg ccgggaagtg gtcgcgcacc gcatccacgt ccagccccgt
     1561 gcgttcgacc tgggtcgtca tcgtccgatg gtacgcggtg tgcgtcgcgg ggacgcaacc
     1621 cgtggccatc cgctagcatc gtgaccatcc atgacgacat ccctgcgcgt gccgacgctg
     1681 cccgggtacg acttcaacct cccgcgaggc ctcgagggcc tcggcgacct cgcctataac
     1741 ctgtggtgga gctggacgcc tcgcgccggc tccgtcttcg cccgcatgga cgctcaggcc
     1801 tggggacgcc accgcaatcc gatccccgtg ctgcgcgcca tggactccaa tcgctgggcg
     1861 gagctgaccg ccgacgagga cttcatggtc gacgtcagcc ggctcctgga cgagtacgag
     1921 cgctacatgg gcaacggcgc ggacacctgg tacggggcgg gcgtcgcgac cgggacgatc
     1981 ccagagctgc ccggcacgat cgcctacttc tgcgccgagt acggcttcca cgagtcgatg
     2041 cagatctact cgggcggcct cggagtgctg gccggcgacc acctcaagtc ggcgtcggat
     2101 gccgcgctgc ccttcgtcgg catcggcctg ctctatcgcc gcggctactt ccgccagcag
     2161 atcgacgccg acgggcacca ggagcacgcc cagccggacc tcgacccggg acagttgcca
     2221 ctgcggcggg cccgcggacc gaacggcggc ccggtccagg tgagcgtgga tttccccggt
     2281 cgccaggtgc acgccgcggt ctgggtggcg cagatcggtc gcgtgccgct gctcctgctg
     2341 gacaccgaca tcccggccaa cgaggagccg gaccggccga tcacccacat cctctacgtg
     2401 cgcggccgcg agatgcggct ttgccaggag ctggtgctcg gcgtcggcgg ggtgcgtgct
     2461 ctgcgccggc tggagatcga gccggcggtc tggcacctca acgaggggca ctccgccttc
     2521 ctgctgctgg agcgcgcccg ggagctcctg gccgagagcc cgggcctcgc gccgaccgag
     2581 gcgctgcgcc gcgtgggcgg cgactcggtg ttcaccatcc atactccggt gcctgccggc
     2641 aacgaagtat tcgagcgcgg tttgatggcc aagaagctgg agccgtggct ggccgagacg
     2701 ggcatgcccg ccggcgagct gatggagctg ggccgcggcc ggactgacga tccggaggcg
     2761 ggcttcgaca tgactgcctt cgtcctgcgt catgcggccg acgccaacgc ggtgagccgc
     2821 ctgcacgcgg agaccgccac ggacacctgg caggaggttg ccggccaccc gatccgcgcc
     2881 atcaccaacg gcgtgcacgt agccacctgg ttgggccgcc ccatccgccg cctctccgag
     2941 cgggccatcg ggacctcgct ggccagcgac gtggccgacg aggggacctt cgccggcctg
     3001 gacggcctgg acgggtccga cctgtgggcc gcccacctgc agcagaagcg ggagctgatc
     3061 gcgtccaccg agcgacggct ggtgagccag ttcgcccgcc acggtgaggc tcctgacgac
     3121 ctgcgcgaga tcgaccacgc cctggacccg aacgcgctga tcatcggctt cgcgcgccgc
     3181 ttcgctacct acaagcgggc ggacctcatc ttcagggacg aggaccggct ggcggcgctg
     3241 ctggccaacc cggagcgccc ggtgcagatc gtgatcgccg gcaaggcgca tcccgctgac
     3301 cgaccgggac agcaggtcat ccagcgcatc ttccagctga cccgatccga tcggctgcac
     3361 ggacgggtct tcatcctgga ggactacgac atgcggatcg cccgcttcct ggtgggcggc
     3421 gtggacatat ggctgaataa tccacgaagg ccgatggagg cctccggcac ctcgggcatg
     3481 aaggcggccc tcaatggcat acccagcgtc agcatcctcg acggctggtg ggacgagggc
     3541 tacaacggca gcaacggctg ggccatcggc gggcgcgggt acgagaccga tgaccccggc
     3601 gcccaggacg cgcaggacgc cgaggacctc taccgcctgc tggagcagga gatcgtgccc
     3661 tgctacttcg accgcaacga ggacggcgtc ccccagggct ggatccgact gatgcgcgcg
     3721 gccatcgggt catccatctg gcagttcagc acgcgccgca tgctcggcga gtacgtcgag
     3781 cagctctacc tgccgaacgt ggcgtcccgc cgtgaggcgg tcccgacgtg atcgcctcct
     3841 gagcgcgtga cacggcggcg taccatccgc agcatgacga cgaccgaggt gacccagggg
     3901 aacacgatga cacccctgca tgcggacctg ctggccctgc tgcacgccat gcgacgcgcc
     3961 gagcgcagca ttttcgccgg cctcagcccg tccgttcgcg acgcgccggg aaccatcggt
     4021 gcctggtcgg ccaaggatgc gcaggctcac ctggccgcct ggcgctcgat cgaggcccgg
     4081 cggctggaag ccaccgtggc aggtggcgag ctgccttcgt ccgatcccgc ccctgacgcg
     4141 ccggtggacg aggccaacgc ccacatccac gagcagcgtg ccggcctggg ctgggatgcc
     4201 gtggagcgcg aggcggacga cagcgtggcc gcgctggccg cggccatcga acgcagcagc
     4261 ttcgacgcgc tctgcgtgtg cgagggaacc accgcgagca tcggagccaa tggcgtgaac
     4321 cacgccatcg cacacctcag cgacgttgcg cgcctgggtg ccagcaaagc ggacttcgac
     4381 gaattcgccc gagaggtgga ggccatcctg ggccgcgggc acctgccggt gcgagactct
     4441 ggcgtcatgc tctacaacct ggcctgccac gcggccctct ccggggacct ggaggacgcc
     4501 cgccggcttc tggccgatgc catcgagcga cggcacgacc tggcggaggt ggcccgcgag
     4561 gatccggacc tcgccgccct cgggccccag gccaggggag tgaccgccta gggcaggatg
     4621 ttccgcgggt tctcccacac cccgtggatg ttggtcgcga agtgcaggtg gcacccggtc
     4681 gagaagccgg tcgtgccctc gtagccgatg gtctgaccgg tggtcaccac ctgtccgggc
     4741 gagacgatca cccgcggcgt gatgtgccag taccacgttt gaatcccccc gccgtggtcg
     4801 atgatcacgc cgtagccggt gtcgtagggc cacagcggct gcccggaggc caccaccacg
     4861 ccggccttgg aggccaggat cggcgtcccg cagccattgg ccatgtcgat cccgccgtgg
     4921 aagtgcgggt agtacgtgcc gtggtaggtg tacggcggct ccagtgcgaa gctggttggt
     4981 ccccattcct gggtgatgcg ggtggacgcc tccggccagc ggaagccgga cgccgagacg
     5041 ttcggcggcc gctgctgctg ctgcgcgcgc ttgcgcgccg cctcggcctc gcggcgccgc
     5101 gcctcctcga gcgccgcctg ctccgcggcc acgcgctgca ccagggcgtt ggcggccgcg
     5161 aacgcctgct cgttggcggc gatctgggcg gagacgtcct gctgggcgtc gagcgcggcg
     5221 ttgagcgccg cctcctgctc ggcacgcttc ttctcggcag ctgccttgag ctccgcggtg
     5281 cgtgcctcca gcttggtcag ctcggcctgc cgctccgcca gcagcgcctc ttcctcggag
     5341 gctgccagcc gcgcggtgcg cagggcgcgg cggccctcgc gcagcgtgtc ctccttgact
     5401 tccagctgct cgcggatcac ccctatctcg tcggcgagct gtcggtcctg ctcggagagc
     5461 gtcagcaggt agccaacctg gttggtggcg gcgtccaggg actcggagct gaggatcacc
     5521 tccagcaggc tggtctggct ctgctcgtac gcggaccgca ggtggtcctg caggagcgcc
     5581 tcgcgcgcgt ggagcgcatc ggtctggtgg gcgacctcgg cggcaaccgc ggcaagctcg
     5641 tcgtccatct cggcgatctg cgccagcagc tcggtcaggc gggcgcggat ctcggccacc
     5701 tgctcacgca cctgcgccag cagcgcaacc acccgctcgt actcggctga tacctcggcc
     5761 agctccgcct cggccacgtc cagctgatcc gcgagcgacg cggagcgaga tttcagctcg
     5821 ctcaggcggg tgcgctgatc cgagagctgc cgctcgatcg aagcgcgctg cgccttagcg
     5881 tcggcgaggg ggtccgcggc cagggccagg tcgcggtggc tgtcggtgag gagcgttcct
     5941 ccgaccatca gcagggcgac cagcgtgacg gccagacgag cgcgtcgcct cattcgagac
     6001 gactccggga gatgttcatt cgacctggtc ccccgttggt tggtgctctc gcagcatgct
     6061 acgcggcctc cggcggcctg tgacgggctc gaatgtacta caccgtagta ctccgatgcg
     6121 gccaaaccgg tacggtcttg cgccgcgggg aacgcctggt gccggcgtgg cgccggccgt
     6181 cagcgaccgc gccgccggcc ggagaggaag cggaccacgc cggcggccac caccccgccc
     6241 accgccaggg accagccggg catcttgcgg ccgcgcgccc cggcgctgcc ggccactggc
     6301 accggaaccg gcgggcggga gggcggccgc tcgccgccca gggctcgctc gccctccatc
     6361 acctcgtcgt ccacgacgtt cgccctcgcc acctcggcgc cgagcagcac cacgatgctg
     6421 gcgaagtaca ggaagacgag cagtgagatc gccgccgaga tgggtccgaa ggccgagtcg
     6481 tagttggcga catcggttgc gtagtaggtg aagccaatgc gcagtgccgt ccacagcacc
     6541 gtcgcgacca ccgcaccggg ccagatctcg gcgatcgaga ccggccggtt gggcaccagc
     6601 cggtagaggg ccaggaaggc gaagaagatc agcaccagcg gggccaggaa gccgatggcg
     6661 gcgaccgcga ctccaccgcc cggtacctgg tccagcacct ccgcggcggc gttctgcagg
     6721 atgccagccg ccacgccgat ggcgaccgag gcaatgccca acagcccggt catgctcatc
     6781 agcgccacgc cgatgagctt gtcctgcagg aacggacgcg gctcggccga gacgaacgcc
     6841 gcgctgatgc ccttgctcac cgcggagaag atgcccaggg cgccccatat caggaagatg
     6901 aggccaaaga tggtgacgcc gccgcgggtc tcgatgatgg agtcgatgac ctcgcccatg
     6961 gtcgcggcgt cgacaggcgt ggcctgctcg atgcgctcca ccacgaactg ccgcgcctcg
     7021 ccctggccga cgaagaagct gagcaccacg acggccagca ccagtagctg gaacagcgac
     7081 aggatggaga agtaggccat gctgccggcg ttctgcagcg cctcgtggcg caggaagccg
     7141 agcagggcgc gacggccgat gcgggtccac cggtgcccga tcagccaggc gtcggccgcc
     7201 ttgagcctgc gcttgatgcc gtcgatcatt ccgctccgcc gtgatcgcaa taggcgtgcc
     7261 gaacaggcta cgctgatcgg catgccgaag gacgcatcgg tccagctgga gctcgacggc
     7321 ttcgacgtca ccgtctccaa ccccggcaag gtcttctttc ccgacgccgg catcaccaag
     7381 atcgaccttg tcaactacta cctggcggtg gccgacggtg cgctgcgcgg cgtgatggat
     7441 cgccccatgg ccctcaagcg cttcgtggac ggcatcagcg cggaggcgtt cttccagaag
     7501 cgcgcgccga aatcggtgcc ggagttcgtg cgcacggtgg agctctcgtt cccgtcgggg
     7561 cgcaccgccg acgaggtggt ggtctcgaac cgggcggcgc tagtgtgggt cgtcaacctg
     7621 ggctgcgtcg acctcaaccc gcacccggtg cgggccgacg acctggccca gcccgatgag
     7681 ctgcgcgtcg acctggaccc cggtcccggc gtggcgtggg acgacgtgcg gcgggtggcc
     7741 atggtggtcc gcgaggtgct ctccgaccac ggcctgactg gctggccaaa gacatccggc
     7801 tctcgtggca tccacgtcta cgcccgcatc gagcgacgct ggggcttctc ggacgtgcgt
     7861 cgcgcggcac tggccctcgc ccgcgaggtg gagcgcagcg cgccggagat ctccacctcc
     7921 aagtggtgga aggaggagcg cctcggcgtg ttcattgact acaagcagaa cgccaaggac
     7981 cgcaccgtgg cctccgccta ctctgtgcgg ccggtcgtgt ccgcgcaggt ctcggctccg
     8041 ttgacctggg acgaggtgcc gcacgccgag ctcggcgact tcacgctgcg cagcatgccg
     8101 gaccgatacg cccgcctggg cgacgtcagt gccgggatcg actcggcggt gggctcgctc
     8161 gagggcctgc tggagctgtc ggcgcgcgac gaggcctccg gactgggaga tgccccctgg
     8221 ccgccgcact acaccaaggc ggagggagag ccgccgcgcg tgcagccctc caagcggcgt
     8281 cggcaggagc cgcgcccgcc gaccgaccca tcggccccgc gcgggcgtgc cggctcggtt
     8341 gcgccggcga ccgtcagcgg ctcctaccgc cgggcggacg gcaccgtttc gcccaccggg
     8401 cgccgtcgct cgcggcatcc gctgctggtg gtggcacagg ccaaggccaa ggccgatgcc
     8461 ctcgccggcc tggaccgatg gaaggcgcgc caccccgagg cggccgtgca cctggcggag
     8521 gacgacgtgc tggtcgacgg catgcgcggc cgttcctcgc tgtggtaccg cgtccgcgtc
     8581 aacctgcgcc atgtccccga aaatgcgcgg ccggatcagg agccacccga cccatactac
     8641 gaccccgccg acgagtggtt gggcgcggag agccgcgact gacccgcggc gtcagcggtt
     8701 caggcgagct gtcagtcgct ccttcaccga tggccactcg tcgtccagga tc
//
LOCUS       DATACA010000017         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1446893, whole genome shotgun sequence.
ACCESSION   DATACA010000017 DATACA010000000
VERSION     DATACA010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446893"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VHK28_00390"
     CDS             <1..955
                     /locus_tag="VHK28_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2140716.1"
                     /translation="MTRRPSPVEWLVFGALGFAWGSSYLFIKIGVETLTPLTLVAGRL
                     AIGAAVLAVVMVLARQALPRHRSAYVHLLGEAMLGIVIPFFLITWGEQSISSALAAIL
                     NGTVPLFAVMLAAVILIDEPITTNRLVGLMVGFAGVILITSPSFATGLDGSLAGQLAL
                     LGASVSYAAAGVYARRFVKGVAPLGSAFLEVGFAFVISLGLAFAFEDPLATRVEVSTV
                     VSVVWLGLIGSGLAFLGFFYLLNRWGATRTSMVAYLLPVVGVVLGVLVRSEAISLPVL
                     AGMVLIIGGVALANMRFGQRRLIGRRQPAAVSQEGAVPTGK"
     gene            complement(980..1330)
                     /locus_tag="VHK28_00395"
     CDS             complement(980..1330)
                     /locus_tag="VHK28_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140717.1"
                     /translation="MPKAGKAMKGVTRTVGKVAGGAGNVAGKAVGTVGDAVGGVIGAV
                     GDALPFGDDKGKSKSSRSGTGTRKTKSSTAKTTTRKTTAKSGGTKATTTKATKATTTK
                     AAAKPHAKRAAKSS"
     gene            1434..4031
                     /locus_tag="VHK28_00400"
     CDS             1434..4031
                     /locus_tag="VHK28_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEX2140718.1"
                     /translation="MANPSRRPRGAVRPKAPEEPRSLDPTEFRWSIVPHTHWDREWYM
                     PFDHFRIRLARTVDEIVETLEGDPAFRRFTLDGQAIILDDYADLRPPERVERLHALAA
                     AGRIAVGPAYVLPDEFLVGAESLVRNFLMGRETCRRHGLEPMPVGYMPDTFGHVAQLP
                     QILRGFGLDTFVFWRGLDDRADALGTVFAWRAPDGSAVAAIRQLGGYGNACQIGAWGA
                     GGADFRDRPEAWPAVAAQRFSRYLDLYGDAVRRGPLDHLLLCNGSDHEPIWAPLAGLL
                     DAARAAHPGLEVSVDSYADFVDAVRPQLTDLPVVDGELVGGREAPVLRGINSARMYLK
                     QSNERTERALSVAETLASLAVLKGTYAYPLHELRVAWRELLKNQPHDSISGCSVDDTH
                     RDMQQRYVTAGAMAERVRREALAALADRVERWSYRPTVGASATVVNPLPQPRARLVNV
                     PIPPDLLDEPELALGTRDGLMAAQREGDVLRASVWMPPWAAEPVSVERGSGRSVGAAR
                     ATGERTIENEFYRVGINPDGWLTVTDRRSGKTWSGLHRFEDVGDRGDEYNFDALPGEA
                     ARSAGFAEVRVRSSGPHVAELEARLTVDLPARLEADRSARSADRVACEISSVIRLVAG
                     VDRVEVVTTVENLAEDHRLRVIFPAPEASEPVRAEGHFDVVRRRARPAWSGSGWVEPP
                     ASTGHTAGAVSVGELVVLTKGLPEYEAIQTANGLEVAITLLRCVGWLSRDDLVSRPGH
                     AGPELPTPGAQCPGRHTFEYAFRFGELDDTALVATSADYRTDFEVGPEGVEPDGVLVI
                     GGDPVAFATMKGAEDGDGVVLRLFNPSAAAADVLISRPATRIRMDEAQPAAGGPRLEP
                     GEIATYRLR"
     gene            4147..>4536
                     /locus_tag="VHK28_00405"
     CDS             4147..>4536
                     /locus_tag="VHK28_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140719.1"
                     /translation="MPAARAPRALLSAVLFVVLLASATTGRAAAADPPSLTGPAPFET
                     RSGQAKGSGSPQATLTTGRLIVQYREGASQAVRAAARAAIGARLVTSVSGTNTELLEA
                     PAGVDEAVVILEHRSDVVSVTPEYRRTL"
BASE COUNT          579 a         1561 c         1651 g          745 t
ORIGIN      
        1 aatgactcgc cgtccctcgc cagtcgaatg gctcgtgttc ggagccctgg gcttcgcatg
       61 gggcagcagc tacctcttca tcaagatcgg cgtcgagacg ctgacgccgc tgaccctggt
      121 ggccggccgc ctggccatcg gtgcggcggt gctggcggtg gtcatggtgc tcgcgcggca
      181 ggcgctgccg cggcatcggt ccgcatacgt ccacctgctg ggggaggcga tgctgggcat
      241 cgtaatcccg ttcttcctga tcacctgggg tgagcagagc atcagctcgg cactggccgc
      301 catcctgaac ggcaccgtcc cgctcttcgc ggtgatgctg gccgcggtga tcctcatcga
      361 cgagcccatc accaccaacc ggctggtggg cctcatggtc ggctttgcgg gcgtgatcct
      421 gatcaccagt cccagtttcg cgacaggtct ggacggttcg ctggccggcc agctggcgct
      481 gctcggcgca tcggtctcct atgccgccgc aggcgtctac gcccgccgtt tcgtcaaggg
      541 cgtggcgccg ctgggcagcg ccttcctgga agttggcttc gccttcgtca tctcgcttgg
      601 cctggccttc gcgttcgagg acccgctggc cactcgggtg gaggtctcga cggtggtgtc
      661 ggttgtgtgg ctggggctca tcggttcagg gctggccttc ctgggcttct tctacctgct
      721 gaatcgatgg ggcgcgaccc gcacctccat ggtcgcctac ctgctgccgg tggtcggcgt
      781 ggtcctgggt gtcctggtgc gcagcgaggc gatcagcctg ccggtgctgg ccgggatggt
      841 gctcatcatc ggtggcgtgg cgctggccaa tatgcgattc gggcagcgcc ggctgatcgg
      901 gcggcgccag ccggccgccg tgtcgcagga gggagcggtt ccgaccggga agtaggcacg
      961 gctcccggcg aggggtcgct cagctgctct tcgcggctcg cttggcgtgc ggcttggctg
     1021 cagccttggt cgtggtcgcc tttgtcgcct tggtcgtggt cgccttggtg ccgccggact
     1081 tcgcggtcgt cttgcgggtc gtcgtcttcg ccgtgctgct cttggtcttg cgcgttcccg
     1141 tgcctgaccg cgacgacttc gacttgccct tgtcgtcgcc gaagggcagc gcgtctccaa
     1201 cggcgccgat cacgccacca accgcgtcac caacggttcc aaccgccttg cccgccacat
     1261 ttccggcacc gcccgcaacc ttgccgaccg tgcgagtgac gcccttcatc gccttgcctg
     1321 ccttaggcat gacctgatcc tccagcgcgt cgcccgctcg tcgggcgatc ttgcgatcgg
     1381 ctagtgcatt cggcgtgccg gggcccctcc gagacaggca caatgcgcag cagatggcga
     1441 acccgagccg ccggccgcgc ggcgccgtgc gcccgaaggc tcccgaggag ccccgatccc
     1501 tcgaccccac cgagttccgc tggtcgatcg tgccgcacac ccactgggac cgggagtggt
     1561 acatgccctt cgaccacttc cgcatccggc tggcgcggac cgtcgacgag atcgtggaga
     1621 ccctggaggg cgacccggcg ttccgccgct tcaccctgga tgggcaggcg atcatcctgg
     1681 acgactacgc ggacctgcgg cctccggagc gggtcgagcg tctgcacgcg ctggccgccg
     1741 ccggccgcat cgccgtgggg ccggcctatg tcctgcccga tgagttcctg gtcggcgccg
     1801 agtccctggt ccgcaacttc ctgatgggac gggaaacctg ccggcgtcac ggccttgaac
     1861 cgatgcccgt cggctacatg ccggacacct tcggccacgt cgcccaactg ccccagatcc
     1921 tgcgcggctt cgggttggac accttcgtct tctggcgcgg cctggacgac agggccgatg
     1981 cgctgggcac cgttttcgcc tggcgcgccc ccgatggctc cgccgtggcc gccatccggc
     2041 agctcggcgg gtacggcaac gcctgccaga tcggggcatg gggtgccggt ggagcggatt
     2101 tccgtgaccg gccggaagcg tggccggcgg tggctgcgca gcgcttcagc cgctacctgg
     2161 acctgtacgg cgacgcggtg cgccgcgggc cgctggacca cctgttgctg tgcaacggat
     2221 cggaccacga gccgatctgg gcgccgctgg ccgggctgct ggacgcggct cgcgcggcgc
     2281 accccgggct ggaggtcagc gtggacagct acgcggactt cgtcgacgcc gtgcggccgc
     2341 agctgacaga cctgccggtg gtcgacggcg agctggtcgg tggccgcgag gctccggtcc
     2401 tgcgcgggat caatagcgcg cggatgtacc tcaagcagtc caacgagcgc accgaacgcg
     2461 cgctgtcggt ggccgagacg ctcgcctcgc tggccgtcct gaagggaacc tacgcctatc
     2521 cgctgcacga gcttcgggtc gcctggcgcg agctgctgaa gaaccagcca cacgactcga
     2581 tcagcggatg ctcggtggac gacacgcacc gcgacatgca gcagcgctac gtgaccgcgg
     2641 gggccatggc cgagcgggtg cggcgcgagg cgctggccgc gctggccgat cgtgtggaac
     2701 gttggagcta ccggccgacc gtcggcgcgt ccgccacggt cgtcaaccca ctgccgcagc
     2761 cgcgggcgcg cctggtgaac gtgcccattc cgcccgacct gctcgacgag ccggagctgg
     2821 cgctcggcac ccgcgacggc ctcatggccg cgcagcggga gggcgacgtg ctgcgggcca
     2881 gcgtgtggat gccgccatgg gcggccgagc cggtcagcgt ggagcgtggc agcgggcgca
     2941 gcgtcggcgc ggcgcgcgcg acaggggagc ggacgatcga gaacgagttc taccgcgtgg
     3001 gcattaatcc cgatggctgg ctgacggtga ccgatcggcg cagtggcaag acctggagcg
     3061 ggctgcatcg gttcgaggat gttggcgacc ggggggacga gtacaacttc gacgcgctcc
     3121 ccggcgaggc ggcccggagc gcgggattcg ccgaggttcg ggtgaggagc agtgggccgc
     3181 acgtggcgga gctggaggct cggcttaccg tggacctgcc ggcacgcctc gaagccgacc
     3241 ggtccgcgcg ctcggcagac cgcgtggcgt gcgagatcag cagcgtcatc cggctggttg
     3301 ccggcgtgga tcgcgtggag gtggtgacga cggtcgagaa cctggcggag gaccaccggc
     3361 tgcgggtgat ctttccggcg ccggaggctt ccgaaccggt tcgggccgag ggccacttcg
     3421 acgtcgtgcg ccggcgggcg aggccggcgt ggagtggatc gggctgggtg gagccgccgg
     3481 caagcaccgg ccacactgcg ggtgcagtat cggtcgggga gctggtcgtg ctcaccaagg
     3541 ggctgcccga gtacgaggcg atccagaccg cgaacgggct ggaggtggcc atcaccctcc
     3601 tgcgctgcgt cggatggctg tcgcgcgacg acctcgtcag ccgccccggc cacgcgggtc
     3661 ctgagctgcc gacgccgggc gcccaatgcc cggggcgtca caccttcgag tacgccttcc
     3721 gcttcggcga gctggacgac acggcgttgg tggcgaccag tgccgactac cgcacggact
     3781 tcgaggtcgg gccggaaggc gtggagccgg atggcgtcct ggtcatcggc ggcgacccgg
     3841 tggccttcgc cacgatgaag ggtgccgagg acggggacgg cgtcgtcctg cgcctcttca
     3901 acccgtcggc cgctgctgcc gacgtgctca tctctcggcc ggcgacgcgc atccgcatgg
     3961 acgaggccca gccggctgcc ggcgggccgc ggctggaacc cggggagatc gccacctatc
     4021 gcctgcgctg agggcgccag cacgcgagcg cacggggttg caccgcccct catccgagga
     4081 atacagtgcg cggggttcgt ggcctcggcc gatgcccgcg gcgcgacgta gggagggata
     4141 cttttcgtgc ctgctgcccg ggccccccgc gcgctgctca gcgccgtgct tttcgtcgtg
     4201 ctcctggcct ctgccacgac cggccgagca gcagccgccg atccgccatc gctcacgggc
     4261 ccggccccgt tcgagacgcg ctcgggtcag gcgaagggat ccggcagtcc gcaggccacg
     4321 ctgaccaccg gccgactcat cgtgcagtac cgggaggggg catcgcaggc ggtgcgcgcc
     4381 gccgcccgcg cagccattgg tgccaggttg gtgacatcgg tctccggcac gaacaccgag
     4441 ctgctggagg cgccggctgg cgtcgacgag gcagtggtta ttctggagca tcggtccgac
     4501 gtggtctccg tcacgccgga gtaccggcgc acgctg
//
LOCUS       DATACA010000018         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2652084, whole genome shotgun sequence.
ACCESSION   DATACA010000018 DATACA010000000
VERSION     DATACA010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652084"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..540
                     /locus_tag="VHK28_00410"
     CDS             187..540
                     /locus_tag="VHK28_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140720.1"
                     /translation="MLVLVAGCGLLEPAPRLVAVPTGAPPPLLGHGCPAALLEGQLVA
                     DDEWGVAVATEFGPQPVRWPNGFYAEREEVLVLRDRRGNQVAAEGEVVYVGGGMDAAN
                     EMFVACDHVSTEPPG"
     gene            669..1175
                     /gene="def"
                     /locus_tag="VHK28_00415"
     CDS             669..1175
                     /gene="def"
                     /locus_tag="VHK28_00415"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX2140721.1"
                     /translation="MVIRRILTAEEPILRERAKKVTQFDASLHRLLDDMLETMRDAPG
                     IGLAANQVGVPLQVAVIELEDKVTELINPQIVKASGEVVDWEGCLSIPGFVAEVARHA
                     KVTVKARDRHGKEFRVKGEELFARALQHEIDHLNGVLYIDYLESLEELVRVSDRPTEV
                     QEEAAAGI"
     gene            1175..>1927
                     /locus_tag="VHK28_00420"
     CDS             1175..>1927
                     /locus_tag="VHK28_00420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2140722.1"
                     /translation="MARLGRLAFLGTGAFGVPLLTRLPELADDLLVVSQPDRPAGRGL
                     QTRPSPVAAWAREQGLRVETPRRIRSDEGRALLRDYAPDGLLLASYGQLVPADLLEIA
                     PRPPLNVHPSLLPRHRGAAPVAGTILSGDRIGGVTLMVMITELDAGPIVAQWGLPLDG
                     SETAPELEARLAELAAEKVPSELGRWVAGTLAPRPQDERAASIIRPFTRADGWIDWRR
                     PAEELDRRVRALQPWPGTWTTLDGRRLHVRAAH"
BASE COUNT          260 a          656 c          714 g          297 t
ORIGIN      
        1 cgggccactc cccgcctacg tggcgcgcag cgctggccgc tggcggttcc aggtgatcct
       61 gcgcgccgag cgcggcgctg accgtcgcgc cgccctggaa cgagtgcccg ccggcgtggg
      121 gatcgacgtg gatcccgagt cgctgctgtg aggcagaggt acgcggtcgc cgccggcctt
      181 gcactcatgc tggtgttggt cgccggctgt gggctgctcg agcctgctcc gcgtctcgtc
      241 gcggttccca ccggcgcgcc accgccgctc ctcggccatg ggtgtcccgc cgcgctgctg
      301 gaggggcaac tggtggccga cgacgagtgg ggggtggcag tggccaccga gttcggacca
      361 cagccggtgc gatggcccaa tggcttctac gccgagcgtg aagaggtcct ggtgcttcgc
      421 gaccgtcgcg gcaaccaggt ggctgccgag ggggaggtcg tttacgtcgg tggtgggatg
      481 gacgcggcca acgagatgtt cgtggcctgc gatcacgtga gcaccgagcc acccgggtag
      541 ctcgtcgagg gcggaccggg gctgccggag gggccggctc cgctgtcagg gccgattgtg
      601 cgcctgtgcg cctgtgcgcc tgtgcgggac cgtccgcccg agcctgcgtc ctctcgtaga
      661 atgacggcat ggtcatccgt cgcatcctga cagccgaaga gcccatcctc cgcgagcgtg
      721 ccaagaaggt gacgcagttc gacgcgtcgc tgcatcggtt gttggacgac atgctggaga
      781 ccatgcgcga tgcgccgggc atcggtctgg cggccaacca ggtcggggtc ccgctgcagg
      841 tcgcggtgat agagctggag gacaaggtca ccgagctgat caacccgcag atcgtcaagg
      901 cctccggcga ggtcgtggac tgggagggct gcctctccat tccgggcttc gtggctgagg
      961 tggcgcggca tgccaaggtg accgtcaagg ccagggatcg gcacggcaag gaattccgcg
     1021 tcaagggcga ggagctcttt gcccgggcgc tgcagcacga gatcgaccac ctcaacggcg
     1081 tgctgtacat cgattacctc gagtcgctcg aggagctggt gcgcgtcagc gaccgcccga
     1141 ccgaggtgca ggaagaggcg gccgcgggca tctagtggcg cgcctgggcc ggctcgcctt
     1201 cctcggcacg ggcgcattcg gcgtcccgct gctcacccgt ctgccagagc tcgctgacga
     1261 cctgctagtc gtcagccagc cggaccggcc ggccggtcga gggctgcaga cccgcccatc
     1321 gccagtggcg gcctgggcac gtgagcaggg gttgcgggta gagacgccgc gacgcatccg
     1381 ctccgacgag ggacgcgctc ttctccgtga ctacgcgccg gacggcctcc tgctcgcctc
     1441 gtacggccag ctggttccgg ccgacctgct ggagatcgcg ccccgtccgc cgctgaacgt
     1501 ccatccctcg ctcctgccgc ggcatcgggg agcggcgccg gtggctggca ccatcctgtc
     1561 cggcgatcgg atcggcggcg tgacgctgat ggtgatgatc accgaactcg acgcggggcc
     1621 catcgtggcg cagtgggggc tgccactcga cggaagcgag acggcgccgg agctcgaggc
     1681 tcgtttggca gagctcgcgg ccgagaaggt gccttccgag ctgggccgat gggtggccgg
     1741 caccctcgcg ccgcggccgc aggacgaacg cgccgcgagc atcatccgcc ccttcacccg
     1801 cgccgacggc tggatcgact ggcggcgccc ggccgaggag ctcgaccgtc gcgtgcgcgc
     1861 ccttcagccc tggccgggga cctggacgac cctggacggc aggcgtctcc acgtgcgcgc
     1921 ggcgcac
//
LOCUS       DATACA010000019         7051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2813231, whole genome shotgun sequence.
ACCESSION   DATACA010000019 DATACA010000000
VERSION     DATACA010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2813231"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VHK28_00425"
     CDS             complement(<1..341)
                     /locus_tag="VHK28_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2140723.1"
                     /translation="MAELNILEAIRTAMDEEMERDERVMVLGEDVGRKGGVFGATDGL
                     WAKYGDKRVLDTPLTEAMIAGAAAGAAIYGLRPIAEMQFADFAYPAFNQIVSEISRFH
                     YRSNGTFSVPM"
     gene            complement(344..1396)
                     /locus_tag="VHK28_00430"
     CDS             complement(344..1396)
                     /locus_tag="VHK28_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2140724.1"
                     /translation="MAKTETRRRHIELGLSDEDVLAMYRQMLLARAVDERMWLMQRAG
                     KIAFIISGQGHEGAQVATAWPLRRNQDWMAPYYRSIASALTFGMSVEDIITAHLAKGD
                     DVSSGGRQMPGHYGGAPYNIVSLSSPVGTQVLHAVGIAMGAWIRGDDVVAMTSYGEGT
                     ANQGEVHEAMNFAGVHNLPVIFVCENNGYAISVPLDRQVGGGNVAVRAQGYGFPGVVV
                     DGSDPLACYAAAREAHERARRGDGPTLIEARVVRLTSHSSDDDQRRYRDPAEVEALKG
                     QDPLARFAAQLRGWDLLTDERDEQLRAEIKAEVNEAGKRAEARPEPSVAEAERRVYAE
                     PIPGGGTPDETPAEGG"
     gene            complement(1413..2831)
                     /gene="lpdA"
                     /locus_tag="VHK28_00435"
     CDS             complement(1413..2831)
                     /gene="lpdA"
                     /locus_tag="VHK28_00435"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX2140725.1"
                     /translation="MTEEQRFDVLILGGGMSYVGAIRAAQLGLKVGLVERDALGGTCL
                     NRGCIPSKALLETADLLHRVSQHGAEFGLAGSDGVSLDYDALGKRRDAVVDKHVKGVE
                     FLMKKNGVTVIRGQGTLTGPTTVRVSGGEAGEVQVSGTDLILATGSAPRSLPGLDPDG
                     ERIITSDEALKRNDVPKRVTIVGAGAIGVEWASMYSDFGAEVTLVEYLDRVVPLEDPD
                     VSKELARVFRKRGIAMHTASTIDPETIQKTDTGLRFTIGQREGDTRTDAEADIILVAV
                     GRRPLTDGIGLESVPGLELDRGYVKVDGNLRTGAEHVYAIGDIVPGFALAHVAAHESV
                     IAAETIAGHDPEPMRSDLMPRVTFCRPQIGSVGMTEAEAREAGHEVKVGSFPFRALGK
                     ATIVGEVDGFAKLVADAQTGLLLGAHVIGPHAGDLLAEPTLARLVEGTASEIAMSVHA
                     HPTLTEVLAEAALAVDGAAIHV"
     gene            complement(2828..3271)
                     /locus_tag="VHK28_00440"
     CDS             complement(2828..3271)
                     /locus_tag="VHK28_00440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX2140726.1"
                     /translation="MSDDDFRHRTSVEVRFKDVDAFGHVNNAVVSTYVEQARVRYLHE
                     VLAVDPVGRMPLILAMIKVDYINPIFWGDQVEVGSRVDWIGRSSIAMSHRLRAGEDRE
                     LARAASVLVTYDYEQARPMPVPDDWRAALEAHEGRSLERSSQGAS"
     gene            complement(3527..4696)
                     /locus_tag="VHK28_00445"
     CDS             complement(3527..4696)
                     /locus_tag="VHK28_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017588375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX2140727.1"
                     /translation="MSLPPGLLPAVQDRYPRYLAELQQLVGIDSGSYSAAGVNRVADF
                     VAASLAELGAEVERVTGGALRDGRPLGDLVIGRLSGEGPRVLLIGHMDTVFDDGTAAE
                     RPFTVRDGRALGPGTSDMKGGLLAGLHALATLREIGSRPAVTFVANPDEEIGSPFSTP
                     HIRRLAADHDVALILECARANGDIVSARKGVADLHITLRGRAAHAGVEPEKGRSAIVA
                     AAHQVLALHALNGRWPGVTVNVGVIGGGTRPNVVPADCLLKVDVRGVTADEFATASAE
                     VERIASAPEVADVTAEVKRVAHHPPMEHSPAAARLAELAISLAGQIGFSLSDTSTGGA
                     SDGNTTASAGLPTLDGLGPIGGDDHSADEWLDLDSVVPRTALLAALIAKVEKGLS"
     gene            4720..6354
                     /locus_tag="VHK28_00450"
     CDS             4720..6354
                     /locus_tag="VHK28_00450"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2140728.1"
                     /translation="MPQQAQPEAGPELLELRVLDGPNRFFTRPAIKLEFAASRPGEAL
                     RTAEAAADAIRRLHAGLDLAPPRVVTRRSVDDRRAMVAFRWRRRSRSQAIAGAAARIA
                     MGSGTVARELRGLRAVAPGPLPALPVPRIPVVAVTGTNGKSTTTRLIAHLATAAGMRV
                     GMTNSDGIHVRGQLVEPGDWTGFGGAARVLAEPDLELAVLETARGGILLRGIGHAVND
                     VGVVTNVSADHLGLQGIDTLDELAEVKATVVRITRRGGWAVLNAQDARVWAMRRETRA
                     GWYAFSLDPAAPGVAEALERGGRAAVLRDGWLVLLASGRRARRLAPVEDLPVTFAGLS
                     EHNVANALAAAAAADALGIRPSRIATGLRSFAMDSEANPGRLNLFTREGTLVVVDFAH
                     NEAGLGGLLRVCRALAAAGERSRPGRVRLALGTAGDRSDEVLHALGALAGQQADDVVI
                     CEKQHYLRGREVETMNEIFRRGAADAGYRRAVRAYPSELDALKALVRRARPGDVAAVM
                     SHAERDAIFAWLAAAGYRPMLPDELRTRLAERGSPA"
     gene            complement(6351..>7051)
                     /locus_tag="VHK28_00455"
     CDS             complement(6351..>7051)
                     /locus_tag="VHK28_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2140729.1"
                     /translation="THLLPATFEGRARMNVQNALAAAGAAWAAGAHLHDIRQGLRTFT
                     TSYFMAPGRLNMFELDGYRVIVDYAHNPPAVRALGEFVDLLAEPTAAGRLPLVTGRRI
                     GVIATAGDRRDEDVLELGATAARFFDAIVVREDANPRGRQRGEIAALIEQGVRTAMAD
                     GARCQSVETIVDEMEATRRALDMGEAGDVVAVCVDHANEVWKELQHRRHGGDAASEFR
                     AVTNVTEDGPEGEF"
BASE COUNT          931 a         2517 c         2549 g         1054 t
ORIGIN      
        1 tccatgggca ccgagaaggt gccgttggac cggtagtgga agcggctgat ctcgctgacg
       61 atctggttga aggccgggta cgcgaagtcg gcgaactgca tctcggcgat gggccgcagg
      121 ccgtagatgg cggccccggc cgcggcgccg gcgatcatgg cctcggtcag cggggtgtcc
      181 agcacgcgct tgtcgccgta cttggcccac agcccgtcgg tggcgccgaa gacgccgccc
      241 ttgcgtccca cgtcctcgcc caggaccatc acccgctcgt cgcgctccat ctcctcgtcc
      301 atggccgtgc gaatggcctc gaggatgttc agctcggcca tggtcagcct ccctccgctg
      361 gcgtctcgtc gggagtaccg ccgccgggaa tcggttcggc gtacacccgg cgctcggcct
      421 ccgccacgct gggctccggg cgcgcctcgg cgcgcttgcc ggcctcgttg acctcggcct
      481 tgatctccgc tcgcaactgc tcgtcgcgct cgtcggtgag gaggtcccag ccccgcagct
      541 gggcggcgaa gcgggccagc gggtcctgcc ccttcagcgc ctcgacctcc gcggggtccc
      601 ggtagcggcg ttggtcgtcg tccgacgagt gcgacgtcag ccgcacgacg cgcgcctcga
      661 tcagggtcgg cccgtcgccg cgccgcgccc gctcgtgcgc ctcgcgcgcc gccgcgtagc
      721 aggccagcgg gtcagatccg tcgaccacga ctccggggaa cccgtagccc tgggcgcgga
      781 cggccacgtt gcctccgccc acctggcggt cgagcgggac gctgatggcg tagccgttgt
      841 tctcgcagac gaagatgacc ggcaggttgt gcaccccggc gaagttcatg gcctcgtgca
      901 cctcgccctg gttggcggtg ccctcaccgt aggaggtcat ggccaccacg tcgtcgccgc
      961 ggatccaggc ccccatggcg atgccgaccg cgtgcagcac ctgggtgccg actggcgagg
     1021 agaggctgac gatgttgtag ggcgcaccgc cgtagtggcc gggcatctgc cggccgccgg
     1081 aggacacgtc gtcgcccttg gccagatggg cggtaatgat gtcctccacg ctcatgccga
     1141 aggtgagcgc cgaggcgatg gagcggtagt agggcgccat ccagtcctgg ttgcgccgca
     1201 gcggccaggc ggtggccacc tgcgcgccct cgtgcccctg gccggagatg atgaaggcga
     1261 tcttgccggc gcgctgcatg agccacatcc gctcgtcgac cgcccgcgcc agcagcatct
     1321 ggcggtacat tgcgagcacg tcctcgtcgc tgaggccgag ctcgatgtgg cggcgtctgg
     1381 tctccgtctt tgccacggtg ccctgatcct ccctagacgt ggatggccgc gccgtcgacc
     1441 gccagggcgg cctcggcgag gacttcggtc agcgtcgggt gcgcgtgcac cgacatggcg
     1501 atctccgagg cggtcccctc caccaggcgc gccagcgtcg gctcggccag caggtcgccc
     1561 gcgtgcgggc cgatgacgtg cgcgcccagc agcaggccgg tctgcgcatc ggccaccagc
     1621 ttggcgaagc catccacctc gccgacgatg gtcgccttgc cgagggcgcg gaacgggaag
     1681 gagccgacct tcacctcgtg cccggcctcg cgggcctcgg cctcggtcat gcccaccgac
     1741 ccgatctgcg gacggcagaa cgtcacgcgc ggcatgaggt cggagcgcat cggttcggga
     1801 tcgtggccgg cgatcgtctc ggcggcgatc accgactcgt gcgccgccac gtgcgccagc
     1861 gcgaagccag gcacgatgtc gccgatggcg tacacgtgct ccgccccggt gcgcaggttg
     1921 ccgtccacct tcacgtagcc gcggtccagc tcgaggccgg ggacggactc caggccgatg
     1981 ccatcggtca gaggccgtct gccgaccgcg accaggatga tgtccgcctc cgcatcggtc
     2041 cgcgtgtcgc cctcgcgctg accgatggtg aagcgcaggc cggtatcggt cttctggatg
     2101 gtctccgggt cgatggtgga ggcggtgtgc atggcgatgc cgcgcttgcg gaagacgcga
     2161 gccagctcct tgctgacgtc gggatcctcg agcggcacga cgcggtccag gtactcgacc
     2221 agcgtgacct ccgcgccgaa gtcgctgtac atgctggccc actcgacgcc gatggcgccg
     2281 gcgcccacga tggtcacccg cttcggcacg tcgttgcgct tgagcgcctc gtcgctggtg
     2341 atgatccgct cgccgtccgg gtccaggccg ggcagggagc gcggtgccga gccggtggcc
     2401 agaatgaggt cggtgccgct gacctgcacc tcgcccgcct cgccacccga gacgcgcacg
     2461 gtggtgggac cggtgagcgt gccctggccg cggatcacgg tcacaccgtt tttcttcatc
     2521 aggaactcga cgcctttgac gtgcttgtcc acgaccgcgt cgcgccgctt gccgagggcg
     2581 tcgtagtcca ggctgacccc gtcgctgccg gccaggccga actcggcccc atgctggctg
     2641 acccggtgca ggaggtcggc ggtctccagc agcgccttgg acgggatgca gccgcggttg
     2701 aggcaggttc cacccagcgc gtcacgttcc accaggccca ccttgaggcc caactgcgcg
     2761 gcgcggatgg cgcccacgta gctcatgccg ccgcccagga tgaggacgtc gaatcgttgc
     2821 tcttcggtca tgaagctccc tgcgacgagc gctccagcga gcggccctcg tgggcctcca
     2881 gcgcggcgcg ccagtcgtcc ggcaccggca tcggccgggc ctgttcatag tcgtaggtga
     2941 ccaggaccga tgccgcccgg gccagctctc ggtcctcgcc ggcacgcagc cggtgtgaca
     3001 tggcgatgct gctgcgaccg atccagtcca cgcgggagcc gacctccacc tgatcgcccc
     3061 agaagatggg gttgatgtag tccactttga tcatggccag gatcaggggc atccggccca
     3121 ccggatccac ggcgaggacc tcgtgcaggt agcggacccg cgcctgctcc acgtaggtgg
     3181 agaccacggc gttgttcacg tgcccgaacg cgtccacgtc cttgaatcgc acctcgacgg
     3241 acgtgcggtg gcggaagtcg tcatcgctca cggagcccag attctacacg ggcccaatgg
     3301 gtgggccgct gagccgttcc tcgacctgcg gcgatttgca ccgggcagca acaactgcga
     3361 ggtagggctc ttccagtagt cgtgggttgc gcggggtgca aacgcgacgc gatcagccgg
     3421 cggccagttg gcgatcattg tcgtggtgcg caaaggaaga ccaggcgcgc gccgctgccc
     3481 cgcagttgtt gccatgggac gcaaacggcg ccgggcgccg gcgacgtcag ctgagccctt
     3541 tttcgacctt ggcgatcagc gcggccagca gggcggtgcg cggcacgacg ctgtcgaggt
     3601 cgagccactc gtcggccgag tggtcgtcgc cgccgatcgg ccccagcccg tcgagggtcg
     3661 ggaggcccgc cgaggcggtg gtgttgccgt cgctggcccc gccggtggag gtgtcgctca
     3721 acgagaagcc gatctggccg gcgaggctga tggccagctc ggccaggcgg gcggctgccg
     3781 gagagtgctc catcggcggg tggtgggcga cccgcttcac ctcggccgtg acgtcggcga
     3841 cctccggggc ggacgcgatg cgttcgacct ctgcggacgc cgtcgcgaat tcgtccgcgg
     3901 tcaccccgcg cacgtcgacc ttgagcaggc agtcggccgg caccacgttc ggccgcgtgc
     3961 cgccgccgat cacccccacg ttcaccgtga cgcccggcca gcggccgttg agcgcgtgga
     4021 gagccaggac ctggtgggcg gccgccacga tcgccgagcg gcccttctcc ggctccacgc
     4081 cggcgtgcgc tgcgcggccg cgcagcgtga tgtgcaggtc ggccacgccc ttgcgcgcgc
     4141 tcacgatgtc cccgttggcg cgggcgcatt ccaggatgag cgcgacgtcg tggtccgcgg
     4201 ccaggcgccg gatgtggggc gtgctgaacg gtgagccgat ctcctcgtcc gggttggcga
     4261 cgaaggtgac cgctggcctc gaaccaatct cacgcagggt cgccagggcg tgcagcccgg
     4321 ccagcagccc gcccttcatg tcgctggtgc ccggcccgag ggcccggccg tcgcgaacgg
     4381 tgaacggccg ctcagcggcc gtgccgtcgt cgaagacggt gtccatgtga ccgatgagca
     4441 ggacgcgcgg cccctctccg ctcagccggc cgatgacgag gtctcccagc ggacgaccgt
     4501 cgcgcagcgc tccccccgtg acccgctcca cctcggcgcc gagctccgcc agtgatgcgg
     4561 ccacgaagtc ggcgacgcgg ttgacgcctg ccgccgagta ggagccggag tcgatgccca
     4621 ccagctgttg cagctcggcc aggtagcggg ggtatcggtc ctggacggcg ggaagcaggc
     4681 cgggcgggag cgacatggcg cgtgagtata ctgccggcca tgccgcagca ggcgcagccc
     4741 gaggccggcc cggagctgct cgagctgcgc gtcctcgatg ggcccaaccg cttcttcacc
     4801 cggcccgcca tcaagctcga gttcgccgcc tcgcggccgg gcgaagcgct gcgcaccgcc
     4861 gaagcagcgg ccgatgccat ccgtcgcctg cacgccggac tcgatctcgc gccaccgcgg
     4921 gtggtgaccc gccgctcggt cgacgaccgg cgtgcgatgg tcgccttccg ctggcggcga
     4981 cgctcgagat cacaggccat cgcgggggcg gcagcgcgga ttgcgatggg atcggggacc
     5041 gtggcccgcg agctgcgtgg cctgcgtgcc gtggcgccgg ggccgctgcc cgcactgccg
     5101 gtgccccgca tcccggtggt ggccgtcacc gggaccaacg gcaagagcac caccacccgg
     5161 ctgatcgcgc acctggcgac cgcggccggc atgcgggtcg gcatgaccaa ctcggacggc
     5221 atccacgtcc gagggcagct ggtcgagccc ggtgactgga ccggcttcgg cggcgcggcc
     5281 cgcgtcctgg ccgagccgga cctcgagctg gcggtgctcg agaccgcccg cggcggaatc
     5341 ctgctgcggg gcatcgggca cgccgtcaac gacgtcgggg tggtgaccaa cgtcagcgcc
     5401 gaccacctcg gcctgcaggg catcgacacc ctggacgagc tggccgaggt gaaggcgacc
     5461 gtggtgcgca tcacccgccg cggtggctgg gccgtgctga acgcgcagga tgcgcgggtg
     5521 tgggccatgc gacgcgagac tcgagcgggc tggtacgcct tcagcctgga tcccgcggct
     5581 cccggcgtcg ccgaggcgtt ggagcgcggc ggtcgggcgg cggtgctgcg tgatggctgg
     5641 ctggtcctgc tggcgtccgg acggcgggcg cggcggctgg cgccggttga ggatctgccg
     5701 gtcaccttcg ccgggctgtc cgagcacaac gtggccaacg ctctggcagc cgcggcggcg
     5761 gccgacgcgc tgggcatccg gccatcgcgc atcgccaccg gactgcgctc gttcgccatg
     5821 gactcggagg ccaatcccgg tcgcctgaac ctcttcacca gggaagggac cctggtggtg
     5881 gtcgactttg cccacaacga ggcggggctg ggcggcctgt tgcgggtatg ccgcgccctg
     5941 gccgccgccg gcgagagatc gcggcccgga cgcgtgcgcc tggcgctcgg caccgccggc
     6001 gaccgcagtg acgaggtgct gcacgccctc ggagcgctgg ccggtcagca ggcggacgac
     6061 gtggtcatct gcgagaagca gcactacctg cgcggccgcg aggtggagac catgaacgag
     6121 atctttcggc gcggggctgc agacgccggc taccggcgtg ccgttcgtgc ctacccgtcg
     6181 gagctggacg ccctcaaggc cctcgtgcgc cgcgcgcgtc ccggtgacgt ggcggcggtc
     6241 atgagccacg ccgagcgcga tgcgatcttc gcctggctgg cagctgccgg ataccgtccc
     6301 atgcttccgg atgagctgcg aacgcggctg gccgaacgcg ggagcccggc ctagaactcg
     6361 ccctccggcc cgtcctcggt gacgttggtc acggcgcgga actcggacgc cgcgtcgccg
     6421 ccgtggcggc ggtgctgcag ctccttccac acctcgttgg cgtggtcgac gcagacggcc
     6481 acgacgtcgc ccgcctcgcc catgtccagc gcgcggcggg tggcctccat ctcgtccacg
     6541 atggtctcca ccgactggca gcgggcaccg tccgccatgg cggttcgcac gccctgctcg
     6601 atgagcgcgg cgatctcgcc acgctgccgg ccgcgggggt tggcgtcctc gcggaccacg
     6661 atggcatcga agaagcgcgc cgcggtggct cccagctcca gcacgtcctc gtcgcggcgg
     6721 tccccggcgg tggcgatgac accgatgcgg cggccggtga ccagcggcag ccggccggca
     6781 gccgtgggct cggccagcag atccacgaac tcgccgaggg cccggacggc cggcgggttg
     6841 tgggcgtagt ccacgatgac gcgatagccg tcgagctcga acatgttcag gcgcccgggg
     6901 gccatgaagt agctggtggt gaaggtgcgc aggccctgcc ggatgtcgtg cagatgcgcc
     6961 cccgccgccc acgccgcacc ggcggccgcc agcgcgttct gaacgttcat gcgggcccgt
     7021 ccctcgaagg tggccggcag cagatgggtg c
//
LOCUS       DATACA010000020         2886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1528018, whole genome shotgun sequence.
ACCESSION   DATACA010000020 DATACA010000000
VERSION     DATACA010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528018"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VHK28_00460"
     CDS             <1..249
                     /locus_tag="VHK28_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2140730.1"
                     /translation="VLVVLATDATLDRAECRKLAELAHDGLARSISPAHTLLDGDVAF
                     ALSTARRPAPDGLRERLTLGSAVVEIVRRAVERSVAAE"
     gene            complement(254..1153)
                     /gene="panB"
                     /locus_tag="VHK28_00465"
     CDS             complement(254..1153)
                     /gene="panB"
                     /locus_tag="VHK28_00465"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2140731.1"
                     /translation="MSSSEQAGGERKIGLAELAAMQRDGRPIVMVTAYDHPSARLADE
                     AGVDIVFVGDSAANNVLGYADTTPISMDEMVMLARAARRGTHRAFFMVDMPLGAFQVS
                     DEDAVRNAVRLVKETGAEAVKIEGAGPMASRARAIVAAGIPVMGHIGLTPQSAGMLGG
                     YKAQGRTAAKARQLFADALALQAAGCFAIVLEAVPDRIAARITEALRTPTIGIGAGPD
                     CSGQVLVWHDLLGINRGHAAKFVKRFADVAGETRRGLETFAAEVRAGTYPADEHTYHI
                     TDAEYALFESEVAPGVLVADDWL"
     gene            complement(1450..2241)
                     /gene="panC"
                     /locus_tag="VHK28_00470"
     CDS             complement(1450..2241)
                     /gene="panC"
                     /locus_tag="VHK28_00470"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2140732.1"
                     /translation="MKVVRRVAELRSALDGVAPPVGLVPTMGAFHEGHLALIRQARTE
                     CRTVVVSLFVNPAQFGPSEDLSRYPRDEARDAALAEAEGVDLLFAPAADEVYPPGFDT
                     WVDPGELGTVLEGAFRPGHFRGVATVCTRLFGLVRPARAYFGRKDAQQVAVMKQVVRD
                     LAIPVEIVTVATVRDPNGLALSSRNVYLSPQEREVALALPRALDEGLRAHRAGGDPVR
                     AARAALEAEPGLSVDYVEVADLDGPTLAAAVRVGSTRLIDNVRLE"
     gene            complement(2238..>2886)
                     /locus_tag="VHK28_00475"
     CDS             complement(2238..>2886)
                     /locus_tag="VHK28_00475"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEX2140733.1"
                     /translation="TGRAGGAIAARLAERGLTVHAGRDAVADAELVLLAVPDAAIAEV
                     AQRVSIGPWVAHVSGATSLSALAPHVRRFCVHPLQSLSRDRGPEQLDGAWAAVTSETD
                     EAAGVAGWLAETLGLRPFPLADGEKPLYHAGAAMASNFLVSLYRAAARLLEETGAPAE
                     ALVPLMQRTIENGFVLTGPIARGDWSTVEAHLRALEERAPDLVPLYRALAKTTRP"
BASE COUNT          395 a         1079 c         1030 g          382 t
ORIGIN      
        1 gtcctggtgg tgctcgccac cgacgccacg ctggaccgcg ccgagtgccg caagctggcc
       61 gagctggcgc atgacgggct ggctcgttcc atctcgccgg cccacaccct gctggacggg
      121 gacgtggcgt tcgcgctatc cacggcacgc cgcccggcgc cggatggact gcgcgagcgg
      181 ctgacgctgg ggagcgccgt ggtcgagatc gtgcgccgcg ccgtggagcg gagcgtcgcc
      241 gccgagtagc tgcctagagc cagtcgtccg cgaccagcac gcccggcgcc acctccgact
      301 cgaagagcgc gtactcggca tcggtgatgt ggtacgtgtg ctcgtcagcc gggtaggtgc
      361 cggctcggac ctcggcagcg aacgtctcca ggccgcgccg cgtctcgccg gccacgtccg
      421 cgaagcgctt cacgaacttg gcggcgtggc cgcggttgat gcccagcagg tcgtgccaga
      481 cgagcacctg gccggagcag tcggggcccg cgccgatgcc gatggtcggg gtgcgcagcg
      541 cctcggtgat gcgtgcggcg atccggtcgg ggaccgcctc gagcacgatc gcgaagcagc
      601 cggccgcctg cagggccagc gcatcggcga acagctggcg agccttggcc gctgtccggc
      661 cctgggcctt gtagccgccc agcatgcccg ccgactgcgg ggtgagaccg atatgcccca
      721 tcaccgggat gccggccgcc acgatggccc gggcccggga ggccatgggt ccggcgccct
      781 cgatcttgac cgcctcagcc ccggtctcct tgaccagccg caccgcgttg cgcactgcgt
      841 cctcgtcgct gacctggaag gcacccagcg gcatgtcgac catgaagaag gcccgatgcg
      901 tcccacgccg ggcggccctg gccagcatca ccatctcgtc catgctgatc ggcgtggtgt
      961 ccgcgtagcc gagcacgttg ttggccgccg aatcgccgac gaagacgatg tccacgccag
     1021 cctcgtccgc cagccgggcg ctggggtggt cgtaggcggt gaccatcacg atcggccgcc
     1081 catcgcgctg catggcggcc agctcggcca gcccgatctt gcgttcgccc cctgcctgct
     1141 cgctgctgct catgcgctca ttgtgccacc ccccgccagc cggaacggag tagccgccgt
     1201 gctcaccagg gggcggcaac cgcgatccag ccggccgatc gcggcgccgt gctcacctgg
     1261 tggccacaac ggccatccag ccggcccatt cggccgttcc ttggccgcca cgctcatctg
     1321 gtgacccagg cggccggcga gggaccgttc tggttggctg cttgtccata atgcccaccc
     1381 ggtggccacg gccgacggcc ctcgggacca ggccgacggg aatcagggcg cggccacacc
     1441 acggcacggt cattccagac gcacgttgtc gatcagccgc gtggagccga cccgcaccgc
     1501 ggcggccagc gtggggccgt ccaggtcggc cacctccacg tagtcgaccg agaggccggg
     1561 ctcggcctcg agtgcggcac gcgccgcccg caccgggtcg ccgccggccc ggtgggcgcg
     1621 gaggccctcg tccagggccc ggggcagggc cagcgccacc tcccgctcct gcggcgagag
     1681 gtagacgttg cgcgaggaga gggccaggcc gttcgggtcc cggacggtgg ccacggtcac
     1741 gatctcgacg ggaattgcca ggtcgcggac cacctgcttc atgaccgcca cctgctgtgc
     1801 gtccttgcgc ccgaagtagg cgcgggcggg gcgcacgagg ccgaacagcc gggtgcagac
     1861 ggtggccact ccccggaagt ggccgggtcg gaacgcgccc tccagcaccg tccccagctc
     1921 ccctgggtcc acccacgtgt cgaatccggg cgggtacacc tcatcggccg ccggcgcgaa
     1981 gagcaggtcg acgccctcgg cctcggccag tgctgcatcg cgcgcctcgt cgcggggata
     2041 gcgcgacagg tcctcggagg gcccgaactg cgccggattg acgaacaggc tgaccacgac
     2101 cgtccggcac tcggtgcggg cctgccggat cagcgccagg tggccctcgt ggaaggcgcc
     2161 catggtgggc accagcccca ccggcggcgc aacgccatcc agcgcggatc gcagctccgc
     2221 gaccctgcgg accaccttca cggacgggtg gtctttgcca gggcgcggta gagcgggacg
     2281 aggtcaggcg cgcgttcctc gagcgcccgc aggtgggcct cgaccgtcga ccagtccccg
     2341 cgggcgatcg gtccggtgag cacgaagccg ttctcgatcg tccgttgcat cagcggcacc
     2401 agggcctccg cgggcgcgcc ggtctcctcg agcaaccgcg ccgccgcccg gtacaggctc
     2461 accaggaagt tcgaggccat ggccgctccc gcgtggtaga gcggcttttc gccatccgcc
     2521 agggggaatg ggcgcagacc gagcgtctcg gccagccagc cggcgacgcc ggcggcttca
     2581 tcggtctcgg aggtgacggc agcccacgcg ccgtccagct gctcaggacc ccgatcgcgg
     2641 gacaggctct gcaacgggtg gacgcagaag cgacggacgt gcggcgccag cgcggacagg
     2701 ctggtggcgc ccgagacgtg cgccacccat ggaccgatgg acactcgctg cgcgacctcg
     2761 gcgatcgccg cgtccgggac ggccagcagc accagctccg cgtccgcgac ggcgtcgcgc
     2821 ccggcgtgga cggtgagccc ccgctccgcc aggcgcgcgg cgatggcgcc cccggcacgc
     2881 ccggtt
//
LOCUS       DATACA010000021         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1367568, whole genome shotgun sequence.
ACCESSION   DATACA010000021 DATACA010000000
VERSION     DATACA010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367568"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VHK28_00480"
     CDS             complement(<1..263)
                     /locus_tag="VHK28_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140734.1"
                     /translation="MAAAALIAEISSPCPPGGALSFGSCEVRPFAAGVVALAVLLYVI
                     GLTAVLGWTRRLRARGADPRGARDWYLIVAVLGLLAAPLLSFTL"
     gene            complement(317..898)
                     /locus_tag="VHK28_00485"
     CDS             complement(317..898)
                     /locus_tag="VHK28_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140735.1"
                     /translation="MTYQPPRRRGPVLRLFRGVDDFTSWMLYGYETWLVAVLKGVALF
                     LFMYFLLFYVPNYVYYAVTLYVLDFSKDVGFLVSNLVGGGNFTALIVMAVWAQAARGR
                     RGFFWSLIRYLVFAQLLLTVLVLIPFMVFNLAGGSLIPPVFTFQAVALGAVAAGLGAL
                     SLVYLWFEYRRVTRREAQALTVAGSSAVAPTGS"
     gene            complement(895..1707)
                     /locus_tag="VHK28_00490"
     CDS             complement(895..1707)
                     /locus_tag="VHK28_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2140736.1"
                     /translation="MRAKTLGVFLMIGVALFTAVYWITDAPRRDARFEELQVELLEYG
                     ETVFGPPTEDHPATANCAQCHGADGTGGEVGDTGVQAPNLHSARIADKLRANPEYVNL
                     VIRFGGVVVSGNVNSLMPAWSTEVGGPLTVEQIDALTSLVESWAMEAAEQPQEEIPDT
                     PEAGQQVYAAAGCGGCHGADLAGTEIAPGLLNIGSEPVVGADLPTPISGEEQLIADYE
                     EDPRMMLELWIRDSAANYNDGESTGMPPHPEGQLSESALQALITFLLEQRQS"
     gene            complement(1712..2365)
                     /locus_tag="VHK28_00495"
     CDS             complement(1712..2365)
                     /locus_tag="VHK28_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2140737.1"
                     /translation="MSIQRSYGEVHGPRAGEMSRRSFMRRMLGVGVGILSLEFLAGTL
                     NFLWPNIREGLGGRLVVGTAADIANQQPDWPAGLPYIFQSKIFIVNVPAAKARVEGEG
                     TVSEPVPDPGTEVSPEIPAVDRSVLALYRKCPHLGCQVPQLCSESQWFECLCHGSKYT
                     VLGEKRDGPAPRGMDRFTLEVDNGVYVINSAELEDGPPIGTETFDTRAPTDMPHCSA"
     gene            complement(2362..2976)
                     /locus_tag="VHK28_00500"
     CDS             complement(2362..2976)
                     /locus_tag="VHK28_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEX2140738.1"
                     /translation="MTIEKRPNATADKNAPTQAPQVPAIQPGTGPKLPGPPGVPGTER
                     FRLLAYVKQESMVRVDKRPDETVLTWPHLLRAEFLMAMLITGLLVITSFFIQAPLEEM
                     ANAGNTPHVAKAPWYFLGLQEMLSYFNATVVGVLFPPLYFIGLMAIAYIDRSPYKAAR
                     DRKLAWVFFMCLMVGGIIVTLIGSFFRGPGWNWVWPWEGIFFTL"
     gene            complement(2992..3711)
                     /gene="extP"
                     /locus_tag="VHK28_00505"
     CDS             complement(2992..3711)
                     /gene="extP"
                     /locus_tag="VHK28_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEX2140739.1"
                     /translation="MSFLVRIVKESSVWRSLFRQPYPTTSRTRALAEMNNVFLHLHPV
                     RVKKHAVRYTYTFCLGGISFFLFLALTVTGVYLMFFYVPSISRAFQDIQTIENSVAFG
                     SLIRNMHRWAAHLMVLTVFLHMIRVFYHGAYKPPREFNWAIGVLLLFSTLWLSFTGYL
                     LPWDQIAFWAITVGTQMATYAPLLSTESSFILLGGIEVGQETLIRFYVMHVIAFPLIA
                     AILMVVHFWRIRKDGGISGPV"
     gene            complement(3708..3854)
                     /locus_tag="VHK28_00510"
     CDS             complement(3708..3854)
                     /locus_tag="VHK28_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140740.1"
                     /translation="MQRCPTNCITMDRLELERFTPDGKVLLESYKDNVLGQIGQQAGM
                     AVDR"
BASE COUNT          697 a         1303 c         1369 g          578 t
ORIGIN      
        1 agggtgaacg acagcaaggg ggctgccagc agcccgagca ccgcaacgat caggtaccag
       61 tccctggcac cgcgcgggtc cgcgccgcgc gcccgcagcc tgcgcgtcca gcccagcacc
      121 gcggtcaggc cgatgacata cagcagcaca gccagcgcca ccacgccggc cgcgaacggc
      181 cgcacctcgc acgagccgaa cgagagcgca ccgcctggcg ggcagggcga ggagatctcg
      241 gcgatgagcg ccgcggcggc caatgcggcg accagcgcgc accaggcgac gtggaccaga
      301 ggcagccgcg aggcggtcag gagccggtgg gagccaccgc cgaggaacca gcgacggtga
      361 gcgcctgcgc ctcgcgccgg gtgacccgcc ggtactcgaa ccacaggtag acgagcgaca
      421 gggcgcccag gcctgccgcc accgccccca gcgccacggc ctggaaggtg aacaccggcg
      481 ggatgagcga gcccccggcc aggttgaaga ccatgaaggg gatcagcacc aggacggtga
      541 gcagcagctg ggcgaagacc aggtagcgaa tcagcgacca gaagaagccg cgccgccccc
      601 gtgccgcctg ggcccatacc gccatcacga tcagggcggt gaagttgccg ccgccgacca
      661 ggttcgagac caggaagcca acatccttgc tgaagtcgag gacgtagagc gtgaccgcgt
      721 agtagacgta gttcggcacg tagaacagca ggaagtacat gaacaggaag agcgcaacgc
      781 ccttcagcac cgccaccagc caggtctcgt agccatacag catccagctg gtgaagtcgt
      841 cgacgccgcg gaagagccgg aggacgggcc cgcggcggcg gggcggctgg taggtcatga
      901 ctgcctctgc tccagcagga aggtgatcag cgcctggagg gcggactcgc tgagctgacc
      961 ctcgggatgc ggcggcatgc cggtgctctc gccgtcgttg tagttcgcgg ccgagtcacg
     1021 gatccacagc tcgagcatca tccgcggatc ctcctcgtag tccgcgatca gctgctcctc
     1081 gccgctgatc ggggtcggca ggtcggcccc caccaccggc tcgctgccga tgttcagcag
     1141 ccccggcgcg atctcggtgc cggccaggtc ggcgccgtgg cagccgccgc atccggcggc
     1201 ggcgtacacc tgctggcccg cctcgggcgt gtccgggatc tcctcctgtg gctgctcggc
     1261 agcctccatg gcccacgact ccaccaggga ggtgagcgcg tcgatctgct caacggtcag
     1321 cggaccgccc acctccgtgc tccaggcggg catcagcgaa ttgacgttgc cggagacgac
     1381 cacgccgccg aagcggatga cgaggttcac gtactcggga ttggcgcgca gcttgtcggc
     1441 gatgcgcgcg ctgtgcaggt tcggcgcctg cacgcccgta tcgcccacct caccgccggt
     1501 gccatcggct ccgtggcact gcgcgcagtt ggcggtggcc gggtggtcct cggtaggcgg
     1561 gccgaacacg gtctctccgt attcgaggag ctcgacctgg agctcctcga agcgcgcgtc
     1621 gcggcgcggg gcatcggtga tccagtagac ggccgtgaag agggccaccc cgatcatcag
     1681 gaagacgccg agcgtcttgg cgcgcatcgg tctaggccga gcagtggggc atgtcggtgg
     1741 gggcccgggt gtcgaaggtc tcggtgccga tgggtgggcc gtcctcgagc tcggcgctgt
     1801 tgatgacgta caccccgttg tcgacctcca gcgtgaagcg gtccatgcca cgcggcgcgg
     1861 gtccatcccg cttctcgccg agcacggtgt acttgctgcc gtggcacagg cactcgaacc
     1921 actggctctc gctgcacagc tgcgggacct ggcagccgag gtgcgggcac ttgcggtaca
     1981 gcgccagcac cgagcggtcg acggccggaa tctcggggct cacctcggta cccggatcgg
     2041 ggacgggctc ggagacggtt ccctcgccct ccacccgggc cttggcggcc ggcacgttca
     2101 cgatgaagat cttcgactgg aagatgtacg gcagcccggc cggccagtcc ggctgctggt
     2161 tggcgatgtc cgcggcagtg ccgaccacca gccgcccgcc gaggccctcg cggatgttcg
     2221 gccacaggaa gttgagggtg ccggccagga actcgagtga caggatgccc acgccaacgc
     2281 cgagcatgcg tcgcatgaag ctgcgccggc tcatctcgcc ggcacgcggc ccgtggacct
     2341 cgccgtagct gcgctggatg ctcatagggt gaagaagatt ccctcccacg gccaaaccca
     2401 gttccagccc ggaccgcgga agaacgagcc gatcagggtc acgatgatgc cgcccaccat
     2461 caggcacatg aagaagaccc atgccagctt tcggtcgcgc gccgccttgt acggcgagcg
     2521 gtcgatgtag gcgatggcca tcagcccgat gaagtagagc ggcgggaaca gcactccgac
     2581 cacggtggcg ttgaagtagg agagcatctc ctgcaggccg aggaagtacc acggcgcctt
     2641 ggccacgtgc ggcgtgttgc cggcgttggc catctcttcc agcggggcct ggatgaagaa
     2701 ggaggtgatc accagcaggc cggtgatcag catcgccatc aggaactcgg cgcgcagcag
     2761 gtgcggccag gtcagcaccg tctcgtccgg ccgcttgtcg acgcggacca tggactcctg
     2821 cttgacgtag gccagcaggc ggaagcgctc ggtgcccggc acgcccggcg ggccgggcag
     2881 cttgggcccg gtgccgggct ggatggcggg cacctgcggc gcctgggtcg gcgcgttctt
     2941 gtcggcggtg gcgttcggtc gcttttcgat ggtcatgagc tcattcgtcc cctagaccgg
     3001 acccgagatg ccgccgtcct tccggatcct ccagaagtgg acgaccatca ggatggcggc
     3061 gatcagcggg aaggcgatga cgtgcatcac gtagaagcgg atcagcgtct cctgcccgac
     3121 ctcgatgccg cccagcagga tgaagctcga ctccgtggac aacaggggcg cgtaggtcgc
     3181 catctgcgtg ccaactgtga tggcccagaa ggcgatctgg tcccacggca gcaggtagcc
     3241 ggtgaagctg agccacaggg tgctgaacag caggagcacg ccgatggccc aattgaactc
     3301 gcgcggcggc ttgtaggcac cgtgatagaa gacgcggatc atgtgcagga agacggtcag
     3361 caccatcagg tgcgcagccc atcggtgcat gttgcggatc agcgagccga aggccaccga
     3421 gttctcgatg gtctgtatgt cctggaaggc gcgactgatg gacggcacgt agaagaacat
     3481 caggtacacc ccggtgaccg tcagcgccag gaacaggaag aagctgatgc cgcccaggca
     3541 aaaggtgtac gtgtagcgca ccgcgtgctt cttgacgcgc accgggtgca ggtgcaggaa
     3601 gacgttgttc atctccgcca gggcgcgcgt gcggctggtg gtcgggtacg gctgccggaa
     3661 caggctgcgc cagaccgatg actccttgac gatgcggacc aggaagctca tcggtccacc
     3721 gccatcccgg cctgctggcc gatctggccc agcacgttgt ccttgtagct ctccaagagc
     3781 accttcccgt caggcgtgaa ccgctcgagc tcgagccggt ccatggtgat gcagttggtc
     3841 ggacagcgct gcatccgctg caacctctgc gtcgagcggt gtccgaccaa ctgcatcacc
     3901 atggaccggc tcgagctcga gcggttcacg cctgacggga aggtgct
//
LOCUS       DATACA010000022         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1287770, whole genome shotgun sequence.
ACCESSION   DATACA010000022 DATACA010000000
VERSION     DATACA010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287770"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VHK28_00515"
     CDS             complement(<1..978)
                     /locus_tag="VHK28_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2140741.1"
                     /translation="MNAPETLVPRLAGIRVAVIGDVMLDSYLDGYAGRLCREAPVPIV
                     SVSERRDAPGGAANTAANVRSLGGEVRLLGITGTDDEAARLRRAMAAADIPHLDLLAV
                     AERSTLAKQRVTAEGQLLLRFDTGSVEPPEGGWAEEIAQRVRELHAWADAIVISDYAY
                     GVVSDAVLTALAELQARRRVPLVGDAKDPRRLVGARPAAVKPNYLEPCALLDLEPLHG
                     IDRRAVQVSEHAERLAELTGAANVVVTLDGEGSVLLRPGEPPYRTYANAVSNDHAAGA
                     GDTFAACLALAMAAGIEPPTGVELASAAAAVAVGKRGTATCTVDELLATV"
     gene            complement(975..1538)
                     /locus_tag="VHK28_00520"
     CDS             complement(975..1538)
                     /locus_tag="VHK28_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX2140742.1"
                     /translation="MTQTDVSRASVTRLACLDKDGTLLVNEPYNVDPARMRPAPDADA
                     ALRLLAGAGFRLAVVTNQPGVGLGRFAAEALLDVRGWLEEYCNRAGVRLDGFYACPHV
                     DGCACRKPRPGLVAAALAAAGANPADSWVIGDILDDVEAGNRAGCRTALVDVGNETEW
                     VRGPHRAPTLVASSLLDAARSIVGATS"
     gene            complement(1535..>1837)
                     /locus_tag="VHK28_00525"
     CDS             complement(1535..>1837)
                     /locus_tag="VHK28_00525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2140743.1"
                     /translation="STAAKRAWPNAAAYHATKWGLLGLSHAMHAEGREMGVKVTAVVA
                     GGMRTPFLLERFPDLDTGLLQDPANVADTIRYVLLQPAETVIPEVMVLPMRETSWP"
BASE COUNT          261 a          683 c          652 g          241 t
ORIGIN      
        1 gaccgtggcc agcagctcgt cgacggtgca ggtcgccgtc ccgcgcttgc caaccgccac
       61 cgcagcggcg gcggatgcca gctccacccc ggtcggcggc tcgatgccgg ccgccatggc
      121 cagcgccagg caggcggcga aggtgtcgcc ggcgccggcg gcgtggtcat tggatacggc
      181 gttggcatag gtccggtagg gcggctcccc tggacgcagg aggacgctgc cctcaccgtc
      241 cagggtcacg accacgttcg cggcgccggt cagctccgcc agccgctcgg cgtgctccga
      301 cacctggacc gctcgccggt cgattccgtg caggggctcc aggtcgagga gcgcgcaggg
      361 ctccaggtag ttgggcttga ccgcggccgg acgagcacca accagccggc gcgggtcctt
      421 ggcgtcgccc accagcggca cgcgacggcg ggcctgcagc tcggcgagcg ccgtcagcac
      481 cgcatcggac accacgccat aggcgtagtc ggagatcacg atcgcatctg cccacgcgtg
      541 gagctcgcgc acacgctgcg cgatctcctc cgcccatccg ccctcaggcg gctcgacgct
      601 gccggtatcg aagcggagga ggagctgccc ctccgccgtc acgcgctgct tggccagcgt
      661 cgatcgctcc gccaccgcca gcagatcgag gtgcgggatg tcggcagcgg ccatagcccg
      721 acgcaggcgc gcggcctcgt catcggtccc ggtgatgccg agcaggcgga cctctccgcc
      781 caggctgcgg acgttggccg ccgtgttggc cgcgccaccg ggggcgtccc gccgctctga
      841 gacgctgacg atgggcaccg gtgcctcccg gcacaggcgg ccggcgtagc cgtcgaggta
      901 gctgtcgagc atgacgtcac cgatgaccgc caccctgatc ccggccagcc gggggaccag
      961 cgtctcgggc gcgttcacga cgtcgctccc acgatggagc gggcggcgtc cagcagggac
     1021 gaagccacca gcgtcggcgc gcggtgcggc ccgcggaccc actccgtctc gttgcccacg
     1081 tccaccaggg ccgtccggca gccggcacgg ttgccggctt ccacgtcgtc caggatatcg
     1141 ccgatgaccc aggagtcggc ggggttggcg cccgcggcgg ccagcgccgc agccaccaac
     1201 ccgggccgcg gcttgcgaca ggcgcagccg tccacgtgcg ggcaggcata gaagccgtcc
     1261 agccgcacac cggcgcggtt gcagtactcc tccagccagc cgcgcacgtc cagcagcgcc
     1321 tctgccgcga agcgcccgag ccccacgccc ggctggttgg tcaccaccgc caggcggaac
     1381 ccggcgccgg ccagcagacg cagcgcggca tcggcatcgg gcgcgggtct catgcgtgcc
     1441 ggatcgacgt tgtacggctc gttaaccagc agcgtgccgt ccttatccag gcaggcaagg
     1501 cgtgtgaccg aggcacggga aacatcggtc tgggtcatgg ccaggacgtc tcccgcatgg
     1561 gcagcaccat cacctcggga atgaccgtct ccgccggctg gagcaggacg tagcgaatgg
     1621 tgtcggccac gttggcgggg tcctggagca gcccggtgtc caggtccggg aagcgctcca
     1681 gcaggaacgg cgtgcgcatg ccaccggcaa cgaccgcggt gaccttgacc cccatctctc
     1741 ggccctccgc gtgcatggcg tggctcaggc ccagcaggcc ccacttcgtg gcgtggtagg
     1801 ccgccgcgtt gggccaggca cgcttggcgg ccgtgga
//
LOCUS       DATACA010000023         4314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1287941, whole genome shotgun sequence.
ACCESSION   DATACA010000023 DATACA010000000
VERSION     DATACA010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287941"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VHK28_00530"
     CDS             complement(<1..534)
                     /locus_tag="VHK28_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2140744.1"
                     /translation="MSPGPPGSVTIVAGATGKLGRAIVARLLDGGHAVIGVSRGRAEA
                     LAGAPPAFEACRADLRDDGCTTALRAAIAGRPVAAVVNAAGAAQRGDVVEVQPEAIGA
                     LVDLKVGGLLRLVRAADPWLVDGSRIVALGGRLGYDADHRAAAAGVANAAVANLVRQL
                     AQAYGPRGVTAHVIAPGA"
     gene            complement(531..1781)
                     /locus_tag="VHK28_00535"
     CDS             complement(531..1781)
                     /locus_tag="VHK28_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2140745.1"
                     /translation="MPLTVARCSIGSSTQPACLGEGEELSVDPGRRRRTLPLAYAGSI
                     IGLTGVQLIVPALPLLQRSLGLSDVQLGVFTAVYLLPGVLLAAPIGFLADRLGRKVLF
                     VASSAVFGLAGGLLFGVHDVPTVFLLRGLQGVAFACLFPLTLTMVGDVTPADGHVREQ
                     GRRSIYMALADVALPMVGAAVVALGTFAPFLVQTSALGLAVLGLLLLPHDRGGYGRPT
                     SATELLRVFRTAPSIALQLAGLYRFLFKFVIYSYGGLLLFDRGFSPQMIGVMVGLLSV
                     ANVLAAAACRAWAYRFPGPRLIALALVAAGAGMAITAAASTAPLLVLAFWLLGLADGL
                     LGVLTNAYMMEMTDSAGRGSYVAMTGAVRNLGKFAAPTLAGGLLWLLPLSGVFGVFAV
                     LAFLAPITLRPLGATASAQPELRR"
     gene            1894..3198
                     /locus_tag="VHK28_00540"
     CDS             1894..3198
                     /locus_tag="VHK28_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140746.1"
                     /translation="MMSLGSSGGLPLLLGHGLGDAGDLPVALPVVLFLAAGSVLLGAW
                     WGDRRPAPSGGEDSGRPLPRLTAFADARATRAVLRTLTVAGLVIVIATAALGPATAAG
                     NPSSRLVFVLFWGLLIPVSLLFGRVWRAVNPYRTIANGLARLAGDPEWNTALPLPQGI
                     GTWPAVVQLALFVWAVIGASERSGQVLVVVLALTAVQLLAANRYGPAWFRHGDPFEVV
                     ADLVASVSPLGRGPDGVLVLRRPVASARRMRLDAGVPVVIALLIGGYLTDFTVDTPLL
                     HELRAPYGRTGQVLVDTLVLAALTAVVLGAIVAVTRRVRFLLPALVPVAAGYALAHDF
                     GVLVVEGQFAFIQLSDPLGRGWDLLGLTGRYVPMEPIPAVIASVVVVLVLVAGHVAAV
                     LLGRSLVGARFSGRTATAVQLRLRAFLVVSVIAGIALRLIVA"
     gene            3450..>4314
                     /locus_tag="VHK28_00545"
     CDS             3450..>4314
                     /locus_tag="VHK28_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX2140747.1"
                     /translation="MTDPSEAPTRPYAEHQRSVLDQMAQAIDAAGPLHSDLPGAPEPL
                     AAAIIAAVEQRSEALIELSHGLHSEPELGYQEHASVRRVARLLEEQGHDCEVGAYGLD
                     TALRASAGSGRPRVAVLAEYDALPGLGHACGHNVICATAVGAFLAVAEVLADLGGSVE
                     LIGCPAEEGGGGKELIARAGGFDGVDAAVMLHPFGADAAVHPWLGVRTVDVTYHGRAA
                     HAAVTPHLGRNALDAVVTAYNGFSQLRQHILPGDRVHGIITDGGQKPNIVPARAAASF
                     FLRSRSIEGLTE"
BASE COUNT          567 a         1560 c         1576 g          611 t
ORIGIN      
        1 ggcgccaggc gcgatgacgt gcgccgtgac cccccggggc ccgtacgcct gcgccagctg
       61 ccgtaccagg ttggcgaccg cggcgttcgc aacgcccgcc gccgccgcgc ggtgatcggc
      121 gtcgtagccg aggcgcccgc ccagcgccac gatcctgctg ccgtcgacga gccagggatc
      181 tgcggcgcgc acgaggcgga ggagcccgcc caccttgagg tccacgaggg cgccgatggc
      241 ctctggctgg acctctacga cgtcgccgcg ctgcgccgcc cccgccgcgt tgacgaccgc
      301 ggccacgggt cgaccggcga tcgctgcgcg cagagccgtg gtgcatccgt cgtccctcag
      361 gtcggcgcgg catgcctcaa acgccggtgg tgcgccggcc agcgcctctg ctcgtccgcg
      421 ggagacaccg atgaccgcgt gcccgccgtc gaggagtctg gccacgatcg cgcgaccgag
      481 cttgccggtc gccccggcga cgatggtgac ggagccgggg gggccggggc tcatcggcgc
      541 agctcgggtt gggcgctcgc cgtggcaccg agcggccgga gggtgatggg cgcgaggaac
      601 gccaggacgg cgaacacccc gaagacgccg ctcagcggga gcagccacag gagccccccc
      661 gccagcgtcg gcgccgcgaa cttgccgagg tttcggacgg ctcccgtcat cgcgacgtag
      721 ctgccgcggc cggcgctgtc cgtcatctcc atcatgtagg cgttggtcag cacgccgagc
      781 agcccgtcgg cgaggcccag gagccagaag gcgagcacca ggagcggcgc ggtgctggct
      841 gcggcggtga tcgccatccc cgcgccggca gctaccagcg ccagggcgat cagccgtgga
      901 ccggggaagc ggtacgccca tgcccggcac gcggccgcgg ccagcacgtt ggccacagac
      961 agcagcccga ccataacgcc gatcatctgc ggggagaagc cacggtcgaa cagcagcaag
     1021 ccgccgtagg aatagatgac gaacttgaac aggaaccggt acaggcccgc cagctggagg
     1081 gcgatcgagg gcgcggtccg gaagaccctg agcagctcgg tggcgctggt ggggcgtccg
     1141 tagccaccgc ggtcgtgagg aagtagcagg agccccagca cggcgagacc gagggcggag
     1201 gtctgcacca ggaagggggc gaaggtaccc agcgccacca cggcggcgcc caccatcggc
     1261 agcgcaacgt cggcgagcgc catgtagatg ctgcgccgtc cctgttcgcg cacatggccg
     1321 tcggcaggtg tcacgtcccc aaccattgtc agcgtcagcg ggaacaggca cgcgaacgcg
     1381 acgccctgga ggccgcgcag caggaacacg gtgggcacgt cgtggactcc gaacagcagc
     1441 ccgcccgcca gtccgaacac ggccgaggag gccacgaaca ggaccttgcg gcccaggcgg
     1501 tcggcgagga agccgatggg tgcggccagc aggacgccgg gcaggaggta gaccgctgtg
     1561 aagactccta gctgcacgtc gctcagcccc agggagcgct gcagcaaagg gagggctggc
     1621 acgatcagct gaacgccggt caggccgatg atgctgcctg cgtaggcaag gggaagcgtc
     1681 cgccttctcc gccctggatc gacgctcagc tcttccccct cgccgaggca agccggttgt
     1741 gtactggatc caatgctgca tcgggcgacg gtcagtggca agggcgcgac ccccgctgcc
     1801 acgccgtatc ggccttcggc ggcgggtgca gcggggtacc gtggagccga tgaacgcgca
     1861 gcgctgttct gcggtgcacg gcggtcgcac gccgtgatgt cgctggggtc ctcgggcggc
     1921 ctgccgctcc tgctgggtca cgggctgggt gacgcgggcg acctgcccgt ggcgctgccg
     1981 gtcgtactgt tcctggcggc tggttcggtg ctgctgggcg cctggtgggg cgaccggcga
     2041 ccagcgcctt cggggggcga ggactccggc cgccccctgc cgcgcctcac cgccttcgca
     2101 gacgctcgtg ccacccgtgc agtgctgcgc acgctgaccg tcgccggcct ggtgatcgtc
     2161 atcgctaccg cggcgctggg tccggcgacg gctgcgggca acccctcgtc acggctggtg
     2221 ttcgtgctgt tctggggcct gctgatcccg gtctcgctcc tgttcggccg cgtgtggcgg
     2281 gccgtcaacc cgtaccgcac catcgccaac gggctcgctc ggctggccgg cgaccccgag
     2341 tggaacacgg ccctgcccct gccccagggg atcggaacat ggccggccgt cgtccagctg
     2401 gcgctgttcg tgtgggccgt catcggtgcc tcggagcgct ccggccaggt gctcgtggtg
     2461 gtcctggcgc tgaccgcggt gcagctgctc gcggccaacc gctacggtcc ggcgtggttc
     2521 cgccacggcg accccttcga ggtcgtcgcc gacctggtcg cgtcggtctc accgctgggt
     2581 cgcgggcccg acggagtcct cgtcctgcgc cgcccggtgg cctcggcccg gcgcatgcgc
     2641 ctcgacgcgg gcgtgcccgt cgtgatcgct ctgctgatcg gcggctacct caccgacttc
     2701 acggtcgaca cgccgctgct gcacgagctg cgcgcgccct acgggcggac gggacaggtt
     2761 ctggtcgaca cgctggtgct ggcggcgctg accgccgtag tcctgggcgc gatcgtcgcg
     2821 gtgacccgca gggtccgctt cctcctgccc gcgctggttc ccgtggcggc cggctacgcg
     2881 ctggcgcacg acttcggggt tctggtcgtc gaggggcagt tcgcgttcat ccagctgagc
     2941 gacccgctcg gccgcggctg ggacctgctg ggtctcacgg gccgctacgt tccgatggag
     3001 ccgatcccgg ccgtgatcgc ctccgtagtg gtggtcctgg tgctggtggc ggggcatgtt
     3061 gcagcggtgc tgctcggccg ctcgctggtc ggggcgcgct tcagcggcag gacggctacc
     3121 gcggtgcagc tgcggctgcg tgccttcctg gtcgtgtccg tgatcgccgg catcgccctg
     3181 cggctgatcg tcgcctgacg ctaccggcac gctccggccg cctgccggcc gcctgccggc
     3241 cgcctgccgc ccgtatggcc cggctgacgc ccctccacgg ttggacagtt ggcccggtat
     3301 ctccggccaa gtgttcaccc gccccagaag cgctgcgctg accgcacagc acgatcccac
     3361 ggttggacag ttggcccggc atcgcgggcc tagtgtccaa cggtccgggg aggctagccg
     3421 ccggagcggt tcgggtagcg tcgacagcca tgaccgaccc ctccgaggca ccgacgaggc
     3481 cctacgccga gcaccagcgc tccgtcctgg accagatggc ccaggccata gacgcggccg
     3541 ggccactcca ctcagacctg cctggcgcgc cggagccgct ggccgccgcg atcatcgctg
     3601 ccgtcgagca gcgctccgaa gcgctcatcg agctctcgca cggcctccac tcggagcccg
     3661 agctgggcta tcaggagcac gcttccgtgc gccgcgtcgc gcgcctgctg gaagagcagg
     3721 gccacgactg cgaggtcggc gcctacgggc tggacactgc cctgcgcgcg agcgcgggct
     3781 cgggccgccc gcgggtcgcg gtgctggctg agtacgacgc cctgccgggc ctcgggcacg
     3841 cctgcggcca caacgtgatc tgcgccaccg cggtcggggc gttcctcgcc gtggcggagg
     3901 tcctggcgga cctcggcggg agcgtggagc tgatcggctg ccccgccgag gaaggtggcg
     3961 gcggcaagga gctgatcgcg cgcgccggcg gcttcgacgg cgtcgacgcg gcggtcatgc
     4021 tgcacccgtt cggggcggac gctgcggtcc acccctggtt gggagtgcgg accgttgacg
     4081 tgacctacca cgggcgcgcc gcgcacgcgg cggtgacccc acacctgggt cgcaatgcgc
     4141 tcgacgcggt cgtgacggcc tacaacggct tttcgcagct gcgccagcac atcctgccgg
     4201 gcgaccgcgt gcacgggatc atcaccgacg gcggccagaa gccgaacatc gtccccgcca
     4261 gggccgcagc ttccttcttc ctccgctcgc gcagcatcga gggcctgacc gagc
//
LOCUS       DATACA010000024         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1930776, whole genome shotgun sequence.
ACCESSION   DATACA010000024 DATACA010000000
VERSION     DATACA010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930776"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VHK28_00550"
     CDS             complement(<1..1682)
                     /locus_tag="VHK28_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX2140748.1"
                     /translation="MELLSSTNQALSQMPPEVQQVGVSVDKSSSGLLLAVNLISPNGT
                     YDSLFLGNYAEIHVADPLARIPGVAEVLNFGFTEYAMRIWLDPDKLANLGMTATDVVN
                     AIGEQNQQVATGALGLPPAPAGQTFTYQLNTLGRLEQVEQFEDIVIRALSNGSVVRIR
                     DVGRVELGSADYSWSTTTSNRPTGNLGIFQLADANGLEIAKEVEATMHRLAQHFPEDL
                     EWEIVYDTTMFVRASITEVIKTMLQAVGLVLLVIFVFLQNWRSTLIPTIAVPVSLIGA
                     FAAMAAFGFTINTLSLLGLVVAVALVVDDAIVVVENVNRRLDEGGKDLREVTELAMTD
                     VRGPIIATTLVLMAVFVPVAFIPGLTGQLYNQFALTIAIAVGISGFNSLTLSPAMCAV
                     LLRPRSGGRKNIAFRAFDTGFEKLTAGYVASVKTCSRVWYLMLLAFAGLCALAVYLFL
                     TIPQGFVPNEDQGYLMTLIELPDASTITRTEEVVTQVNDAALSTPGVSATVAVAGYNI
                     VDGIQQPNAGIAFIVLEPWDQRTTTETSAPGILAALQEKYNQIAGASVTAVDA"
BASE COUNT          289 a          533 c          582 g          291 t
ORIGIN      
        1 gcgtccacgg cagtcacgct ggcgccggcg atctggttgt acttctcctg caaggcggcc
       61 aggatcccgg gtgctgacgt ctcggtggtg gtgcgctggt cccacggctc caggacgatg
      121 aaggcgatgc cggcgttcgg ctgctggatg ccgtcgacga tgttgtagcc ggcgaccgca
      181 accgtcgcag agacgccggg cgtgctcagc gcggcgtcgt tgacctgggt gacgacctcc
      241 tcggtacggg tgatcgtcga cgcgtcgggc agctcgatca gggtcatcag atagccctgg
      301 tcctcgttgg gtacgaagcc ctgcgggatg gttaggaaca ggtagactgc cagcgcgcag
      361 agtccggcga acgcgagcag catcaggtac cagacgcgcg agcaggtctt gacgctcgcg
      421 acgtagcccg ccgtgagctt ctcgaaccct gtgtcgaagg cgcgaaaagc gatgttcttc
      481 ctgccgcccg accgtggccg caggagtacc gcgcacattg ccgggctcaa ggtcagcgag
      541 ttgaagccgg agatgccgac cgcgatcgca atggtcaggg cgaactggtt gtacagctgg
      601 cccgtcagcc ccgggatgaa ggccaccggc acgaacactg ccatcagcac cagggtggtg
      661 gcgatgatcg ggccccgcac gtcggtcatc gccagctcgg tgacctcccg caggtccttg
      721 ccgccctcgt ccaggcggcg attgacgttc tccaccacga cgatggcgtc atcgaccacc
      781 agcgccacgg cgacaacgag accgagcagg ctcagcgtgt tgatcgtgaa gccgaacgca
      841 gccatcgcgg cgaaggcgcc gatcaaggac accggcaccg caatggtcgg gatcagcgtg
      901 ctgcgccagt tctgcaggaa gacgaagatg accagcagca ccagcccgac cgcctgcagc
      961 atggtcttga tgacctcggt aatcgaggcc cgcacgaaca tggtggtgtc gtagacgatc
     1021 tcccactcca ggtcttcggg gaagtgctgc gcgaggcggt gcatcgtcgc ctcgacctcc
     1081 ttggcgatct ccagaccatt ggcgtcggcg agctggaaga tgccgaggtt gccggtgggc
     1141 ctgttgctcg tcgtcgtcga ccagctgtag tcggcggagc cgagctcgac cctgccgacg
     1201 tcccggatgc gcaccacgga gccgttggac agtgcgcgga tgacgatgtc ctcgaactgc
     1261 tcgacctgct cgagccggcc cagggtgttg agctggtagg tgaaggtctg cccggcaggc
     1321 gccggcggca gcccgagcgc accggtcgcg acctgctggt tctgctcgcc gatcgcgttc
     1381 acgacgtcgg tcgcggtcat gcccaggttg gcgagcttat ccggatcgag ccagatccgc
     1441 atcgcatact cggtgaagcc gaagttgagg acctcggcca cgcccgggat ccgcgccagg
     1501 gggtcggcca cgtggatctc tgcatagttg ccaagaaaga ggctgtcata ggtgccgttg
     1561 ggcgagatga ggttgacggc cagcagcagg ccggaggagc tcttgtcgac gctcacgccg
     1621 acttgctgga cctccggcgg catctgtgag agcgcctggt tcgtgctgct cagcagctcc
     1681 atctgggcga tgttc
//
LOCUS       DATACA010000025         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_83296, whole genome shotgun sequence.
ACCESSION   DATACA010000025 DATACA010000000
VERSION     DATACA010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83296"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..1146)
                     /locus_tag="VHK28_00555"
     CDS             complement(298..1146)
                     /locus_tag="VHK28_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2140749.1"
                     /translation="MPRTIRAALLQAEWTGDKESMIEKHEKYAREAAAQGAKVMCFQE
                     LFYGPYFCQVQDPQYFSYTEHIPDGPTTKRMQELARETGMVLIVPMYEEDEDTTGIYY
                     NTAAVIDADGKYLGKYRKTHIPHVKGFWEKFYFRPGNMGYPVFDTAVGKVGVYICYDR
                     HFPEGARALGLNGAELVFIPSATSRGLSEYLWRVEQVSHALANGYYVGTINRVGVEPL
                     GDDDFYGQSYFVDPRGQFVGDVGDPHKEELIVRDLDMDKITEVRQLWQFYRDRRPDAY
                     EDLVRP"
     gene            1270..2445
                     /locus_tag="VHK28_00560"
     CDS             1270..2445
                     /locus_tag="VHK28_00560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HEX2140750.1"
                     /translation="MNLIDLLLVLLLLLGVVAGARAGFLGPVLGLTGGVLGLVLALAL
                     ASVFQPQLGQIEQPTRALVTLLGLGALVIGGEALGAAFGSIASHRLRRSWFQPFDAAG
                     GAVVGALHVVLVAWVLTGLLAAGLIPAMSEAARGSAAVAAIGRSLPAPGAVAGRLLSL
                     LDTTDLPPLFAGLEPPPAAPVELPADADARALAQSALASTVKVSGGGCGAWLQVGSGF
                     FIGPSHVVTNAHVVAGTDSSSVTLGGTTYRASVVHFDPDADLALLHVPEAVAPALQLS
                     PQAPERGQTAVALGFPGGGELMVSPAAVTAVHHVQAPDIYGEDHAERIVVEMRADVRR
                     GNSGGPLLVAPGVVGGVVYGESRSFPDVGYAIAASQAAATIGPSVGSTQAVDTGPCG"
     gene            2469..3080
                     /locus_tag="VHK28_00565"
     CDS             2469..3080
                     /locus_tag="VHK28_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2140751.1"
                     /translation="MPWSPPAKIAFIGSHSVRKSNAVHAFASTIGRAGRSVEVGREVV
                     RFNPLGLNEAATPEAQLWVLMAQVQQELELRNRAEVLVTDRSVVDNFAYYLRVTDGAD
                     PFAVEPLIRSWAATYDLLVRLLPDVALLADGVRSVNDAFRDEIEAILDRILPEYLPPE
                     RLLTVRASEVTADFDWWAVAEGLAARVGQPLVEGQLSAELVRG"
     gene            3109..>3318
                     /locus_tag="VHK28_00570"
     CDS             3109..>3318
                     /locus_tag="VHK28_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140752.1"
                     /translation="MLTARLAGFLNVPILRRVAFHARRIGNQVDRHFFTTLLTAVLGF
                     VVAATIVVTLLERQLTLADFGNTFYW"
BASE COUNT          444 a         1171 c         1154 g          549 t
ORIGIN      
        1 cctctcgctc gaggatggcc gacgcgggcg cggtgggcaa tgcctcgccc ttcatgaatc
       61 ccccgatggg tcgctggttg gagccctgat ggtagacgga cgcgcaaggc ggtgagacca
      121 gcccccgccg tgcgaaccgt cctgattcac accaggcacg cgttaggcgc ggaatccggc
      181 ccctgaaggg tcgggattca cacccggcgt gaattcagac gcttgaacgc tgcgccacag
      241 cgcgtatggg tcatgtggcg tgaaatcgga gcgccgaggc ccggctcagg acgcggccta
      301 cggccggacc aggtcctcgt aggcgtcagg ccggcggtcg cggtagaact gccagagctg
      361 ccggacctcg gtgatcttgt ccatgtccag gtcgcggacg atcagctcct ccttgtgggg
      421 gtcgccgacg tcgccgacga actggccacg cggatccacg aagtaggact ggccgtagaa
      481 gtcgtcgtca cccagcggct ccaccccgac acggttgatg gtgccgacgt agtagccgtt
      541 ggccagcgcg tgcgacacct gctcgacccg ccacaggtac tcgctcaacc cgcgcgaggt
      601 ggccgatgga atgaacacga gctccgcccc attcagcccc agcgcccgcg ctccctccgg
      661 gaagtgacgg tcgtagcaga tgtagacgcc caccttgccg accgcggtgt cgaacaccgg
      721 gtagcccatg ttgccgggac ggaagtagaa cttctcccag aagcccttga cgtgcgggat
      781 gtgcgtcttg cggtacttgc ccaggtactt cccgtcggca tcgatcaccg cggcggtgtt
      841 gtagtagatg ccggtggtgt cctcgtcctc ctcgtacatg gggacgatca gcaccattcc
      901 cgtctcacgc gccagctcct gcatgcgctt ggtggtcggg ccatcgggaa tgtgctcggt
      961 gtagctgaag tactgcgggt cctgcacctg gcagaagtac gggccgtaga agagctcctg
     1021 gaagcacatg accttcgcgc cctgggccgc cgcctcgcgc gcgtacttct cgtgcttctc
     1081 gatcatggac tccttgtcgc ccgtccactc ggcctggagc agggcggcgc ggatggttct
     1141 cggcatcgac gatcggcctc ctcggagtga ctcgagatgt gctcaggcgc cgatactaca
     1201 ccggccccag ctgaggccca cggcccgatt cctgctcagg gtcccaatcc gccacacttg
     1261 ccctcaccga tgaacctgat cgacctgctt ctcgtcctgc tgctcctgct gggggtggtg
     1321 gccggtgccc gcgccggatt cctcggcccg gtgctgggcc tgaccggtgg ggtcctcggc
     1381 ctggtgctgg cactggccct ggcatcggtc ttccagccgc agctcgggca gatcgagcag
     1441 cccacccggg cgctggtcac gctcctggga ctcggcgcgc tggtcatcgg cggcgaggcg
     1501 ctgggagccg ccttcggctc gatcgccagc caccgcctgc gacgcagctg gttccagccc
     1561 ttcgacgccg ccggcggggc ggtggtcgga gcgctccacg tggtgctcgt ggcctgggtc
     1621 ctgaccgggc tgctggccgc cggcctgatc cctgccatga gcgaggcggc gcgcgggtcc
     1681 gcggcggtgg ccgccatcgg acgcagcctg ccggcgccgg gcgcggtcgc cggtcgcctg
     1741 ctgtccctcc tcgacaccac cgacctgcca cccctgttcg ccgggctgga gccgccgccg
     1801 gccgcgccgg tcgagctgcc ggccgatgcg gacgcgcgcg cgctggcgca gtccgcgctg
     1861 gccagcaccg tcaaggtctc cggcggcggc tgtggggcct ggctgcaggt gggcagcggc
     1921 ttcttcatcg gtccgtcgca tgtcgtcacc aatgcgcacg tggtggccgg gacggactcc
     1981 tcctcggtca cgctgggcgg tacgacgtac cgggcgagcg tggtgcactt cgatccggac
     2041 gccgacctgg cattgctcca cgtccccgag gccgtcgcgc cggcgctcca gctcagccca
     2101 caggcgccgg agcgggggca gacggcggtg gccctgggct tccccggcgg cggcgagctg
     2161 atggtcagcc cggcggcggt gacggccgtc caccacgtcc aggcgccgga catctacggc
     2221 gaggaccacg ccgagcgcat cgtggtcgag atgcgtgccg acgtgcgacg cgggaactcc
     2281 ggcgggccgc tgctggtggc gcccggcgtg gtcggcggcg tggtctacgg cgagtcgcgg
     2341 tcgttcccgg acgtcggcta cgcgatcgcc gcatcgcagg cggccgccac catcggtcca
     2401 tccgtgggca gcacccaggc ggtcgacacc gggccctgcg gatagcggat ggacggcaca
     2461 atgccctcat gccctggagc ccgcccgcca agatcgcgtt catcggctcg cactcggtgc
     2521 gcaagtcgaa cgcggtgcac gcgttcgcct cgaccatcgg ccgtgcgggc cgcagcgtcg
     2581 aggtcgggcg cgaggtggtg cgcttcaacc ccctcggcct gaacgaggcg gccactcccg
     2641 aggcccagct ctgggtcctg atggcccagg tgcagcagga gctcgagctg cgcaaccggg
     2701 ccgaggtgct ggtcaccgat cgatcagtgg tcgacaactt cgcgtactac cttcgcgtca
     2761 ccgatggcgc cgacccgttc gccgtggagc cgctcatccg gtcctgggcc gcgacctacg
     2821 acctcctggt gcggctgctg ccggacgtgg cgctgctggc ggacggcgtg cggtcggtga
     2881 acgacgcctt ccgcgatgag atcgaggcga tcctggatcg catcctgccc gagtacctgc
     2941 cgcccgagcg gctgctgacg gtgcgtgcct cggaagtcac cgccgacttc gactggtggg
     3001 cggtggccga gggcctggcg gcgcgggtcg ggcagccgct cgtcgaaggg cagctctccg
     3061 cggagctggt tcgcggctga cggccgcgag atacactccg cgccaccgat gctgactgcc
     3121 cgcctcgccg gcttcctcaa cgtacccatc ctgcggcgcg tcgcgttcca cgcaaggcgc
     3181 atcgggaacc aggtcgaccg gcatttcttc acgaccctgc tgacggcggt gctgggcttc
     3241 gtcgtggcgg cgaccatcgt ggtgaccttg ctcgagcgcc agctcacgct ggccgacttc
     3301 ggcaacacgt tctactgg
//
LOCUS       DATACA010000026         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2091493, whole genome shotgun sequence.
ACCESSION   DATACA010000026 DATACA010000000
VERSION     DATACA010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2091493"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="VHK28_00575"
     CDS             complement(<1..1744)
                     /locus_tag="VHK28_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX2140753.1"
                     /translation="MRRTTMLTFTRIFALGAALLCAAWLSMALPGTVLAEEPPELNEP
                     VTDLAGVLDGGERSEAEDGIQRLEDEANVQMFAVFVNDTEGMAAPEYATETARLNGLG
                     GNDVVLVVSFEPRRYGIWVGDALTDVSDDEIDTLLVEHVEPALREEDFGGAVAAGADY
                     LNAAAESGAPAEPQEPGGGGGTPAPDDGDGPPWGAILGIGLLVVGGVVMWRWWQGRQA
                     AGRDEEERDRRLGKLTREANALLIETDELIRHDAQELGFAEAQFGEEAAATFAKALDA
                     AREELRAAFTVRQRLDDNVPEAPQEREQLLNEIVTRCTRAQELLEEQTRRFEELRDLE
                     RRAPELLAGTEAAATRLEGRIAEAEHSIGQLRAEAPSAAAVVEGHPAEARKRIGLARE
                     AARQGTDALAADDRSAAGRAARAAQEAIAQATTLLDAVDRERKGLATAQAELSQALAQ
                     ARTDVQAAVRAVSADGELARVATEARETLDRADRLASGPDRDVVLAHRLAIEAEAAAD
                     RVVATVREGEERRAKEVAGARAQLRSAEQSLRRAEDYVQARRHGIGRRPRTRLSEARE
                     AFAAARGLVDSDPAA"
     gene            1830..>2217
                     /locus_tag="VHK28_00580"
     CDS             1830..>2217
                     /locus_tag="VHK28_00580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2140754.1"
                     /translation="MADGCCTTDYDRIFDETSARKELTDYLDHGLDDDTSRLLNALVA
                     QDVQGSSVLEIGGGVGDVQLELLAAGGASVTDVDASQPYIAVAREEVERRGLADRVRY
                     VHGDFVAVASGVPPADVVVLNRVVCCY"
BASE COUNT          284 a          854 c          766 g          313 t
ORIGIN      
        1 cggccgccgg gtcactgtcc accagcccgc gcgccgcggc gaaggcctcg cgagcctccg
       61 acaggcgggt gcgggggcgg cgcccgatgc cgtggcggcg cgcctgcacg tagtcctcgg
      121 cgcgacgcag cgactgctcc gcgctgcgca gctgggcgcg tgcgccggcc acctccttcg
      181 cgcggcgctc ctctccctca cgcaccgtgg ccacgacccg atcggccgcc gcctcggcct
      241 cgatcgccaa ccgatgggcc agcaccacgt cacggtccgg gccgctcgcc agccggtcgg
      301 cgcgatccag ggtctcgcga gcctccgtgg caaccctcgc cagctcgccg tcggctgaca
      361 cggcacgcac ggcggcctgc acatcggtcc tggcctgggc cagcgcctgc gacagctccg
      421 cctgggcggt ggccagcccc ttgcgctcgc gatcgaccgc gtccagcagg gtcgtggcct
      481 gggcaatcgc ctcctgggcg gcccgagccg cgcggccggc agcagatcgg tcatccgcgg
      541 ccagcgcatc ggtcccctgg cgggcagcct cgcgtgccag cccgatccgc tttctcgcct
      601 cggccggatg accctccacc acggccgccg cggagggcgc ctcggcccgc agctggccga
      661 tggagtgctc cgcctccgcg atgcgaccct ccaggcgggt ggccgccgcc tcggtcccgg
      721 ccaggagctc cggcgcccgg cgctccaggt cccgcagctc ctcgaagcgg cgggtctgct
      781 cctccagcag ctcctgggcg cgcgtgcagc gcgtcacgat ctcattgagc agctgctccc
      841 gctcctgcgg cgcctccggc acgttgtcgt ccagccgctg gcgcacggtg aaggcagcgc
      901 gtagctcctc ccgtgcggcg tcgagcgcct tggcgaaggt ggccgccgcc tcctcgccga
      961 actgcgcctc ggcgaagccg agctcctgag cgtcgtgccg gatgagctcg tcggtctcga
     1021 tcagcagcgc gttcgcctcg cgggtcagct tgcccagtcg gcggtcgcgc tcctcctcgt
     1081 cgcgcccggc agcctgccgc ccctgccacc agcgccacat gaccacgccg cccaccacca
     1141 gcaggccgat gcccaggatc gcgccccacg gcggcccgtc gccatcgtcc ggcgccgggg
     1201 tgccgccgcc accgccgggt tcctgcggtt ccgcgggcgc accgctttcc gcagcagcgt
     1261 tgaggtagtc ggcgccggca gccaccgccc cgccgaagtc ctcctcgcgc aacgccggtt
     1321 cgacgtgctc gaccaggagc gtgtcgatct cgtcgtcgga gacatcggta agggcgtcgc
     1381 cgacccagat gccgtaacgg cggggctcga agctgaccac cagcacgacg tcattgccgc
     1441 cgaggccatt caggcgtgcc gtctcggtcg cgtactccgg ggcggccatc ccctcggtgt
     1501 cgttcacgaa cacggcgaac atctgaacgt tcgcctcatc ctcgaggcgc tggatgccat
     1561 cctcggcctc cgagcgctct ccgccgtcca gcacgcccgc gaggtcggtg accggctcgt
     1621 tgagctccgg cggctcctcg gccagcaccg tgccgggcag cgccatcgag agccacgcgg
     1681 cacagagcag cgcggcaccc agggcaaaga tccgggtgaa agtaagcatg gtggtgcggc
     1741 gcatcaccgc cagattagtg ccgagcagcg tcgattgcag aagtgccaaa cgtcgtgatt
     1801 tcttggccgc gccgcgtaga ctcgacggca tggctgacgg gtgctgcacc accgactacg
     1861 accgcatctt cgacgagaca tcggcgcgca aggagctgac cgactacctg gaccacgggc
     1921 tggacgatga cacctcccgg ctgctcaacg cgctcgtcgc gcaggacgtc cagggcagca
     1981 gcgtgctgga gatcggcggc ggggtgggcg acgtacagct ggagctgctg gccgcggggg
     2041 gggccagcgt gaccgacgtg gacgcctcgc agccgtacat cgcggtggcc cgcgaggagg
     2101 tggagcgccg cggcctggcc gaccgggtga ggtacgtgca tggcgacttc gtggcggtgg
     2161 ccagcggcgt gccacccgcc gatgtggtcg tgctcaaccg cgtggtgtgc tgctacc
//
LOCUS       DATACA010000027         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1930869, whole genome shotgun sequence.
ACCESSION   DATACA010000027 DATACA010000000
VERSION     DATACA010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930869"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VHK28_00585"
     CDS             <1..331
                     /locus_tag="VHK28_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140755.1"
                     /translation="VTTEELEPHLETAGRYVGIAGLVREPWELQTIEGRLAWVRPEDN
                     ATAAGTMIYTWAADDLVFLMIGVNDTVNRALFAALPGEAAATPVPSPSGTPAGRTSEP
                     SGTASPS"
     gene            364..1641
                     /locus_tag="VHK28_00590"
     CDS             364..1641
                     /locus_tag="VHK28_00590"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEX2140756.1"
                     /translation="MPVRRILSQVRNSLWFVPGLWVVLAGTLAFGTLAVDSAAPELAR
                     SVELLFGGSPEGARSVLESIAGSMITVAGVVFSITIVALQLASSQLSPRVLRNFMRDR
                     RSQSVLGALIGTFFYSLLVLRAIRTGGDGRDAFVPSLAVTVAIVLAAVAVAMLVFFIH
                     HIARRIQVSTIVDSIATETLEAIGKEWRGDQDETSGAATAPPGLPGSVPATTSGYLQL
                     VDTDGLLDTAAELDLVVRVEARPGDWVQAGTPLFAVWPMEAGGQRIHERLNQQVTLGA
                     ERSLEQDAGFGIRQLVDVAVKALSPGINDPTSARDCINRLAQLLIAIGQAPSRSASLS
                     KDDRLRVILSLRDFGDLVDLAFDELRHYAGSTPAVAIALADALSTVRSQVDVGLRGPL
                     ERQARLLLECADEIRPDGDRERVAASVQRVLQP"
     gene            1673..2752
                     /gene="cax"
                     /locus_tag="VHK28_00595"
     CDS             1673..2752
                     /gene="cax"
                     /locus_tag="VHK28_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEX2140757.1"
                     /translation="MLSRIAYGVSILGTIATLVLRFTGADETLTFGVSALTILGLAYT
                     LGHATEQLGLAAGPRIGGIMNATVGNIGEIIIAAFLILDDKIAIVHASITGSIVGNLL
                     LVLGASLLAGGLRNGIQTYDAQTTGMNAASLILATIGLIVPATFAFLVGGGEGTGPGD
                     PQFFQIEALTIGVAILLLTTYAAQIWFFLRSPESPTTAHAPDETAHWSWRMSLAVLLA
                     SAAALTFSSEVLVHTMEPAVASLGISEFFIGIILIPLIGNLAEHVVGITLAYKNKMDF
                     SLITSLGSATQIALFAVPVLVFFGLIVGNPVTLVFSPLEVVAVGTGVLIASYIALDGK
                     SNWVEGLQLCSVYLILAIAFFFLTP"
     gene            2820..3848
                     /locus_tag="VHK28_00600"
     CDS             2820..3848
                     /locus_tag="VHK28_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX2140758.1"
                     /translation="MEAWPGLDEGDGREDRWARKRDRLARLLSVASILYSRGSGESGV
                     AVTEIAELTGMTTRTIYRDIRALEEELGMPIFQAGRGRYGIEKKYFLPPLRLSLPEAV
                     VLFLASRLIARWSDEYDQAVISAFTKLADLLPQPIARHVAATMLRVGRADPNEPFTRN
                     FAQVAQAWADGRVIEFDYEPAGGERRSARVRPYFLEPDAAGRSIYLIGWDEKVEAMRT
                     YKVERIRASTLTADRYEIPEDFDPDRWLAHSWGIWSSDGTEVVEVVLRFDRSVAHRVR
                     ESIWHRSQRLEDLADGGVEMRLSVAGIVEIRPWILTWGDAVEVMEPPALREAVAAAVR
                     KAAARYGT"
     gene            3848..4489
                     /locus_tag="VHK28_00605"
     CDS             3848..4489
                     /locus_tag="VHK28_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX2140759.1"
                     /translation="MPGELELELKYRADGEESLRALAAIARLGEAELGPPSAVDEVDR
                     YLDTPSGDLSRARWACRLRTREGRTIVSLKGPAQHAAGDALHRRPEVEGPAGSGLDPA
                     AWPDSPARDLLMRLSGSARPLAERVVLQQRRTEREARLGGRPLGTLSLDVVSVRHADG
                     PLGVLHLVELELAAHAEPLADALAAALAARQGLQADPHSKLEHALALLDAVRP"
     gene            4486..>5949
                     /locus_tag="VHK28_00610"
     CDS             4486..>5949
                     /locus_tag="VHK28_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2140760.1"
                     /translation="MSPRRAGEIAALGLGIAVFGYLGWDGALWDARLQLLLHLAAVAA
                     VAALLGLAWAGGSLPRTRLDVPILVLLVAFGLGALTAWNHGLSARALASILATAAMLP
                     VALLVLRHRAGWAALVVTLPVLALSAGSLAVLGWRRVEWLLAGGPGLPPIRLGGEGTP
                     FGSVAVPPFVILASLPLALLIPQPRLRLGVLLGLAAVGVPLTLLSGSRSAWLAILATA
                     VVLLAPRLSGRLPRIDWRGEWTPRRTALAFGGAVLAILAVVYVAPRLLEAGSLAYRGF
                     LWRDTLAAWSADPLLGIGPGAMPFARQVAAPPLTFPARQPHSHDVPLGILGDAGLVGL
                     VAALVVVVVFVRLAGPWATRTIPGRAAFAVLAGFGVGMLFEDLTFLPNFNLMVILLAG
                     MALTDAGAVAWERPRPDVLGVRGLRAARVTAVMGALALGLVLVLGDGAAIAYREGVEA
                     YNQERWTDAAAHLATSGGLDPWQPTTPKALALAADRSG"
BASE COUNT          764 a         2125 c         2109 g          951 t
ORIGIN      
        1 cgtgaccacc gaggagctcg agccgcacct ggagaccgcg ggccggtacg tcggcatcgc
       61 ggggctggtg cgcgagccct gggagctgca gaccatcgag ggcaggctgg cctgggttcg
      121 ccccgaggac aacgccaccg cagccggcac catgatctac acctgggccg ccgacgacct
      181 ggtgttcctc atgatcggcg tcaacgacac cgtcaaccgc gccctgttcg cggcgctccc
      241 cggcgaggcg gcggcgaccc cggtgccctc gccgagtggc acgccggcgg gccgcacctc
      301 ggagcccagc ggcaccgcct caccttcctg atcccggccg cgatcgtgca tcggtgaagg
      361 ccgatgcccg ttcgtcgcat cctgagccag gttcgcaaca gcctgtggtt cgtcccgggc
      421 ctttgggtcg tgctggccgg gaccctcgcc ttcgggacac tggccgtcga ctcggctgcc
      481 ccggagctgg cccgttcggt cgagctgctc ttcggtggga gcccggaagg agcacgcagc
      541 gtcctggagt cgatcgccgg ctcgatgatc accgtggccg gcgtggtctt ctcgatcacc
      601 atagtcgccc tgcagctcgc ctcctcgcag ctgtctcccc gcgtgctgcg caacttcatg
      661 cgcgatcgcc gcagccagtc ggttctcgga gcgctgatcg gcacgttctt ctactcgctg
      721 ctcgtcctcc gagcgatccg taccggaggc gacggccggg atgcgttcgt cccgtcattg
      781 gccgtcacgg tggcgatcgt gctcgccgcg gtggcggtgg ccatgctggt cttcttcatc
      841 caccacatcg cgcggcgtat ccaggtctcg accatcgtcg acagcatcgc caccgagacc
      901 ctggaggcca tcgggaagga atggcgcggg gaccaggacg agacgtcggg tgctgccacg
      961 gccccgcccg gtctgccggg atccgtaccg gccacgacgt ccggctacct gcagctcgtc
     1021 gataccgacg gcctgctgga caccgccgcc gagctggacc tggtggtacg ggtcgaggcg
     1081 cggcccggcg actgggtgca ggccggaacg ccattgttcg ccgtatggcc catggaggcc
     1141 ggcggccagc ggatacatga gcggctcaac cagcaggtga cactgggcgc cgagcggtcc
     1201 ctggaacagg acgccggatt cggcatccgc cagctcgttg acgtggcggt caaggccctg
     1261 tcgcccggca tcaacgaccc gacgtcggca cgcgactgca tcaaccgcct ggcgcagctc
     1321 ctgatcgcca tcgggcaggc accctcgcgc tcggcgagcc tgtccaagga cgatcggctg
     1381 cgggtgatcc tgagcctgcg cgacttcggt gacctggtcg acctcgcctt cgacgagctg
     1441 cggcactacg ccggcagcac tccggccgtg gccatcgccc tggcggacgc gttgagtacg
     1501 gtgcgcagcc aggtcgacgt cgggctgcga gggccgctcg agcgtcaggc tcgcctcctg
     1561 ctggagtgtg cggacgagat ccgtccggac ggcgaccgcg agcgggttgc agcatcggtg
     1621 cagcgcgtgc tgcaaccatg agcggaggcg cgtccggcta ccatcggcaa ccatgctgag
     1681 ccgcatcgcc tacggcgtct cgatcctggg caccatcgcc accctggtcc tgcgattcac
     1741 cggggccgac gagacgctca ccttcggcgt cagcgccctc accatcctgg ggctggccta
     1801 cacgctcggg cacgccaccg agcagctggg cctggccgcc gggccgcgca tcggcggcat
     1861 catgaacgcc acggtcggca acatcggcga gatcatcatc gccgccttcc tgatcctgga
     1921 cgacaagatc gccatcgtgc acgcctcgat caccggctcg atagtcggca acctgctgct
     1981 ggtgctcggt gcctcgctcc tggccggggg gctgcgcaac gggatccaga cctacgacgc
     2041 gcagaccacc ggcatgaacg ccgcgtcgct gatcctggcc accatcgggc tgatcgtgcc
     2101 ggccaccttc gccttcctgg tgggcggtgg cgaggggacc gggccgggcg acccgcagtt
     2161 cttccagatc gaggcgctca ccatcggggt cgccatcctg ctgctgacca cctacgcggc
     2221 gcagatctgg ttcttcctgc gctccccgga gtcgccgacg accgcgcacg cgccggatga
     2281 gaccgcgcac tggagctggc ggatgtcgct ggcagtgctc ctggcttcgg ccgccgcgct
     2341 gaccttctcc agcgaggtgc tggtccacac catggagccg gcggtcgcct cgctgggcat
     2401 cagcgagttc ttcatcggca tcatcctcat cccgctgatc ggcaacctgg cggagcacgt
     2461 ggtgggaatc accctggcct acaagaacaa gatggacttc agcctgatca cgtccctggg
     2521 ctcagcaacg cagatcgccc tgttcgccgt gccggtgctc gtcttcttcg gcctcatcgt
     2581 cggcaacccg gtgacgctcg tcttcagccc gctggaggtg gtggcggttg gaaccggcgt
     2641 gctcatcgcc agctacatcg ccctggacgg caagtccaac tgggtggagg ggctccagct
     2701 gtgcagcgtg tacctgatcc tggccatcgc cttcttcttc ctgacgccgt aggacggtca
     2761 ggcgaccgtg cggggacaag gtgatcatcg gtctatcatc ggctgccatg gagggaccga
     2821 tggaggcctg gccgggcctg gacgaaggtg acggccgcga ggaccgatgg gcgcgcaagc
     2881 gcgaccgcct ggcgcgcctg ctgagcgtcg cctccatcct gtactcacgc ggctccggcg
     2941 agtcgggcgt ggcagtcacg gagatcgccg agctgaccgg gatgaccacc cggacgatct
     3001 accgcgacat ccgggcgctc gaggaagagc tcggcatgcc gatcttccag gctggacgcg
     3061 ggcgctacgg catcgagaag aagtacttcc tgccgccgct gcggctgtcg ctgccggagg
     3121 ccgtggtcct gttcctggcc tcgcggctga tcgcgcgctg gtcggacgag tacgaccagg
     3181 cggtgatcag cgccttcacc aagctggccg atctgctgcc gcagccgatc gcccgccacg
     3241 tggcggccac catgctgcgc gtcggccgcg ccgacccgaa cgagccgttc acgcgcaact
     3301 tcgcgcaggt ggcgcaggcc tgggccgatg gacgggtcat cgagttcgac tacgagccgg
     3361 ccggcggtga gcggcgctcc gcgcgggtgc gcccctactt cctggagccg gatgcggccg
     3421 ggcgcagcat ctacctgatc ggctgggacg agaaggtgga ggccatgcgc acctacaagg
     3481 tggagcgcat tcgcgcctcc accctgaccg ccgaccgcta cgagatcccg gaggacttcg
     3541 acccggaccg atggctcgcg cactcgtggg gcatctggtc gtccgacggc accgaggtgg
     3601 tggaagtcgt cctgcgcttc gaccgatcgg tggcgcaccg ggtgcgcgag tcgatctggc
     3661 accgctccca gcgcctggag gacctggccg atggcggcgt ggagatgcgg ctgtcggtgg
     3721 cgggcatcgt cgagatccgg ccatggatcc tgacctgggg agacgcggtc gaagtcatgg
     3781 agcctcccgc cctgcgtgaa gcggtggcag ccgcggtccg caaggccgcc gcccgctacg
     3841 ggacctgatg cccggcgagc tcgagctgga gctgaagtat cgggcggacg gggaggaatc
     3901 gctgcgggcg ctggcggcca tcgctcggct cggcgaggcg gaactcggcc cgccgagcgc
     3961 cgtggacgag gtcgaccgct acctggacac cccgtccgga gatctgtccc gggcgcgctg
     4021 ggcctgccgg ctgcgcacgc gggagggacg caccatcgtg tccctgaagg gacccgcgca
     4081 gcacgcggcg ggcgacgcgc tgcatcggcg cccggaggtc gaaggcccgg ccgggagcgg
     4141 cctcgaccct gcggcctggc ccgactcgcc ggcccgtgac ctactgatgc gcctcagcgg
     4201 tagcgcgcga ccactcgccg agcgggtggt cctgcagcag cgaaggacgg agcgcgaggc
     4261 acgcctgggt ggacggccgc tgggcacgct ctcgctggac gtggtctcgg tgcgccacgc
     4321 tgacgggccg ctcggcgtcc tgcacctcgt cgagctggag ctggcggcgc atgccgagcc
     4381 actggcggat gcactggcgg cagcgctcgc ggcacggcag gggctgcaag cagacccgca
     4441 ctccaagctt gagcacgcgc tggccctgct cgacgccgtt cggccatgag cccacgccgc
     4501 gccggggaga tcgctgccct gggcctgggg atcgccgtct tcggctacct gggctgggac
     4561 ggagcgctgt gggatgcgcg gctgcagctg ctcctccacc ttgccgcggt ggccgccgtc
     4621 gccgcgctgc tgggactcgc ctgggctggc ggctcgctgc cgcgcaccag gctggacgtc
     4681 ccgatcctgg tcctgctcgt cgccttcggc cttggagcgc tgaccgcctg gaaccacggc
     4741 ctctccgccc gcgccctggc atcgatcctg gccaccgctg ccatgctgcc ggtcgccttg
     4801 ctcgtactgc gccaccgggc ggggtgggcg gcgctggtcg tcactctgcc ggtcctcgcc
     4861 ctgtcggcgg gctcgctggc ggtcctcggc tggcgccggg tggagtggct gctggccggc
     4921 ggcccgggcc tgccgccgat ccggctcgga ggggagggaa cgcccttcgg ctcggtggcg
     4981 gtaccgccgt tcgtgatcct agcgtccctg ccgctggcac tgctcatccc gcagccgcgg
     5041 ctccggctcg gtgtcctgct cggtttggcg gcggtcggcg tgccgctcac cctgctctcc
     5101 gggtcgcgat cggcatggct cgccatcctg gcaaccgcgg tggtgctgct cgcgccgcgg
     5161 ctgagtggac gcctgccgcg catcgactgg cgcggcgagt ggacgcctcg gcggacagcc
     5221 ctggcgttcg gcggggccgt gctggccatc ctggccgtgg tctacgtcgc cccgcggctg
     5281 ctggaggccg ggtcgctcgc ctatcgcggc tttctgtggc gcgacacgct ggctgcctgg
     5341 tccgccgacc cgttgctggg gatcggcccg ggagcaatgc cattcgcgcg acaggtggcc
     5401 gcgcctccgc tcaccttccc ggcccgacag ccacactcgc atgacgtgcc gctcggcatc
     5461 ctgggcgacg cagggctcgt cgggctggtc gcggcgctgg tcgtggtggt cgtcttcgtg
     5521 cggctggccg gaccgtgggc cacgcggacg atacccgggc gcgccgcctt cgccgtcctg
     5581 gccgggttcg gggtgggcat gctgttcgag gacctcacct tcctccccaa cttcaacctg
     5641 atggtgatcc tgctggccgg catggccctg accgatgccg gcgcggtcgc ctgggagcgc
     5701 cctcgtccgg atgtgctggg cgtccgcggg ctgcgcgccg ctcgcgtcac cgccgttatg
     5761 ggcgcgctgg ccttgggact ggtgctcgtg ctcggcgatg gcgccgcgat cgcctaccga
     5821 gagggggtcg aggcctacaa ccaggagcgc tggacggacg ccgccgcgca cctggcgaca
     5881 tcgggcgggc tcgacccgtg gcagcccacc acccccaagg cgttggccct ggccgcggat
     5941 cgcagcggc
//
LOCUS       DATACA010000028         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1770398, whole genome shotgun sequence.
ACCESSION   DATACA010000028 DATACA010000000
VERSION     DATACA010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770398"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VHK28_00615"
     CDS             <1..1112
                     /locus_tag="VHK28_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2140761.1"
                     /translation="SSQCGPPTAYRNPTWESGLPTIATSGLTFVDGEPWGDWDGQLFV
                     SQLKEKDMRRFSLSPSGTTSTQQEIHFDGDWGRLRAATRGPAWQLYLSTSNGSNDLIV
                     RVSVSEPSVGRIFGADRYATAAAVSEATTAQDVPVAYVASGIDFADALTGGAAAAHLD
                     GPVLLVTPNTIPEATAAELTRLTPGRIVVLGGPKAVSATVAEGLMAYTAGSVSRLAGA
                     DRYETAARVATTSFAPGVDAAFVATGSGFADALAGVPAAAHLEGPLLLTKRIGLPAAT
                     ENALDALNPARIYVLGGPAAVSDAVLASLDHLTTGPVTRLAGADRFATAAAVASLWPR
                     SNAAYVATGAEFPDGVSGGAAAARQDVPLLLTEQN"
     gene            1113..1238
                     /locus_tag="VHK28_00620"
     CDS             1113..1238
                     /locus_tag="VHK28_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140762.1"
                     /translation="MPTVTGQALLRLHPTAVTVLGGSSAVSDAAAADIAGLLAIP"
     gene            1277..>2571
                     /locus_tag="VHK28_00625"
     CDS             1277..>2571
                     /locus_tag="VHK28_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2140763.1"
                     /translation="MPPDTAATVAPRTPDEILARLNPPQAEAVRHVSGPLLILAGAGS
                     GKTRVLAHRVAYLVAIGVKPWQIVAVTFTNKAANEMRERIAGLIGPEAAREATIGTFH
                     AICARILRRDGDKIGLTRSFTIYDRADQVAVVKQMLRALDLDEKRFSPAGMLAWIGQR
                     KDELADVPTARRQAANFYDETAARVYEAYQRQLHEDDAVDFDDLLMRTVFLFEQHPDV
                     LARYQGRWRQVLVDEYQDTNRAQYLLCQLLAAKHRNLAVVGDDDQSIYSWRGANLRNI
                     LDFEKDYPDAHVVKLEQNYRSTQTILDAAHAVVSRNEGRKDKRLWTDRGAGTQISIFD
                     AYNEYEEAEFVARQVERLVGGSGSGGLSGLLTRRADDEDGALRYGEIAVCYRINAQSR
                     VLEEAFMRFGIPYQLVGGTRFYERREVKDALAYVRLARNP"
BASE COUNT          380 a          944 c          882 g          365 t
ORIGIN      
        1 cgagctcgca gtgtggcccg ccgacggcgt accgcaaccc gacctgggag tccgggctgc
       61 cgaccatcgc cacctcgggg ctgaccttcg tggacggaga gccctgggga gactgggacg
      121 gacagctttt cgtctcgcag ctcaaggaga aggacatgcg ccgcttctcg ctgtcgccga
      181 gcggtaccac ctccacccag caggagatcc atttcgacgg ggactggggc cggctgcggg
      241 cggcgacgcg cgggccggcc tggcagctgt acctgtccac ctcgaacggc agcaacgatt
      301 tgatcgtccg cgtctcggtg tccgagccgt ccgtggggcg gatcttcggc gccgatcgct
      361 acgccacggc cgctgccgtg agcgaggcga ccacggcgca ggacgtcccg gtggcctacg
      421 tcgccagcgg catcgacttc gccgacgcgt tgaccggcgg ggccgccgcg gcgcacctgg
      481 acggcccggt gctgctcgtc acgccgaaca ccatcccgga ggcgaccgcc gccgagctga
      541 cccggctcac gcccggacgg atcgtggtgc tggggggccc gaaggccgtc tcagcgaccg
      601 tcgccgaggg gctgatggcc tatacggcgg gcagcgtctc gcgcctggct ggtgccgacc
      661 gctacgagac cgccgcccgg gtcgccacca ccagcttcgc gccgggcgtg gacgccgcct
      721 tcgtggccac cggcagcggc ttcgccgacg cgctggccgg tgtccccgcc gcggcccacc
      781 ttgaaggacc gctgctcctc accaagcgga tcgggctgcc ggcagccacc gagaacgcgc
      841 tcgatgcgct caacccggcc cgcatctatg tcctcggcgg cccagccgcc gtttccgacg
      901 ccgtcctcgc ctcgctggac cacctgacca ccggcccggt gacccgcctg gcgggtgccg
      961 accgcttcgc cacggcggcg gcggtggcct cgctgtggcc ccgctcgaac gcggcctacg
     1021 tggccaccgg cgccgagttc ccggatggcg tctccggcgg cgcggcggct gcgcgccagg
     1081 acgtgccgct cctcctgacc gagcagaatt agatgcccac ggttaccgga caggcgctcc
     1141 ttcgcctgca tccgaccgcc gtcaccgtgc tgggcggcag cagcgcggtg agcgatgcgg
     1201 ccgctgccga catcgccggg ctgctggcca tcccctgagc cggctgcccg gtcggccgct
     1261 cgctacaatc gacggcatgc cccccgacac cgcggccacc gtcgcgccgc gcactcctga
     1321 cgagatcctc gcccggctga acccgccgca ggccgaggcg gtcaggcacg tctcggggcc
     1381 cctcctcatc ctggccgggg ccggaagcgg caagacgcgc gtcctggccc atcgggtcgc
     1441 ctatttggtg gccatcggcg tcaagccgtg gcagatcgtg gcggtcacct tcaccaacaa
     1501 ggcggccaac gagatgcggg agcggatcgc cgggctcatc ggtccggagg ctgcgcgcga
     1561 ggcgaccatc ggtacctttc atgccatctg cgcgcgcatc ctccgacgtg acggtgacaa
     1621 gattggcctg acacgctcgt tcaccatcta cgaccgtgcg gaccaggtgg cggtcgtcaa
     1681 gcagatgctc cgcgcccttg acctggacga gaagcgcttc agcccggcgg gcatgctggc
     1741 ctggatcggc cagcgcaagg acgagctggc ggacgtgccc accgctcgcc gacaggccgc
     1801 caacttctac gacgagaccg cagcgcgggt ctacgaggcc taccagcgcc agctgcacga
     1861 ggacgatgcg gtcgatttcg atgacctgct gatgcgcacc gtgttcctct tcgagcagca
     1921 cccggacgtg ctggccaggt accagggccg ctggcgccag gtcctggtgg acgagtacca
     1981 ggacaccaac cgcgcccagt acctgctctg ccagctgctg gccgccaagc atcgcaacct
     2041 ggcggtggtc ggcgacgacg accagtccat ctactcgtgg cgcggcgcca acctgcgcaa
     2101 catcctggac ttcgagaagg actacccgga cgcccacgtg gtgaagctgg agcagaacta
     2161 ccgctccacg cagaccatcc tggacgcggc gcacgcggtc gtcagccgca atgaggggcg
     2221 caaggacaag cgcctgtgga ccgatagggg ggccggcacc cagatctcga tcttcgacgc
     2281 ctacaacgag tacgaggagg cggagttcgt ggcgcggcag gtcgagcgcc tggtgggtgg
     2341 ctcgggctcc ggcggcctgt ccggcctgct cacacgccgg gccgatgacg aggacggcgc
     2401 gctgcgctac ggcgagatcg cggtctgcta ccgcatcaac gcccagagcc gggtgctgga
     2461 ggaggcgttc atgcgcttcg gcatcccgta ccagctggtc ggcggcaccc gcttctacga
     2521 gcggcgcgag gtcaaggatg cgctggccta cgtgcgcctg gctcgcaacc c
//
LOCUS       DATACA010000029         4696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_806488, whole genome shotgun sequence.
ACCESSION   DATACA010000029 DATACA010000000
VERSION     DATACA010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806488"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VHK28_00630"
     CDS             complement(<1..1286)
                     /locus_tag="VHK28_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2140764.1"
                     /translation="MATTTLDPRAGTAGRSWVIDWLTTVDHKKIGILYIVNSFLFFFA
                     AGILALVIRSELAVPGLQFVDRDTYNQAFTMHGTIMIFLFIVPVLAGFGNYIVPLQLG
                     APDMAFPRINALSFWILPLGGLMIFSGYLFGGAAAEGWTMYTPLSTEDYAPQVGTDLW
                     LIGLALVGTASILGAINFIATVFKMRAPGMTLFRMPIFVWTVLVTATLILLATPVLTA
                     GLIALFIDRNYGGAFFDPAAGGNPILWQHIFWFFGHPEVYIVILPAMGVVSEILPVFS
                     RKPLFGYRAFVFATVGIGLLSFSVWAHHMFTTGEVFLLFFAFMTALIGVPTGVKMFNW
                     IFTMWRGKLRLSTPLLFALGFLSMFLIGGINGAFSASVPVDFAIHDTYWVVAHIHYVL
                     FGGSVFGIISEVISANARKPVFGYRMMAFSLMAIVLL"
     gene            complement(1303..2367)
                     /gene="coxB"
                     /locus_tag="VHK28_00635"
     CDS             complement(1303..2367)
                     /gene="coxB"
                     /locus_tag="VHK28_00635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2140765.1"
                     /translation="MQFPAPRSRMRTMLRVGAPLLLILLLGGCLLPPEPKTEAGESVF
                     NLYLITLVLAAIVFVGVEGFILYAVFRYRRKPGDEVLPEQHHGNNLIEIIWTAIPTVI
                     VLTLFTFSMLSLAEVEARVEGDEGVQIEVDGFQWSWQFHYQDPEVTIGGTAADPPVMV
                     VPVGEPVVLTLTTRDVNHSFYVPDFLIKRDLIDFGPQREPNELRFTVDEVGTYAGQCA
                     EFCGTQHADMTFVVEARERADYDAYLADLAAGEEPQEPAAGDCAVTVDIGAENVEFDI
                     SEFEVPADTAFCINFENREDIPHNVSIYDGTEALFTGDILDEAGTITYQVPALPAGEY
                     QFICDVHPDTMVGDVIVTSQ"
     gene            complement(2526..3566)
                     /locus_tag="VHK28_00640"
     CDS             complement(2526..3566)
                     /locus_tag="VHK28_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140766.1"
                     /translation="MAAASERRISPIVAGIRWNRREASGVTGAMNHLRPSMATLLVVL
                     LALSACASGGAPGGAEVSADPSAPDTDDGGGGGIEHPIGADEAILVVDAVGGFVPVDF
                     LVTQMPSFSLFGDGRVVMMGMQTLEFPGPALPALIERRLTEDGIQAILEAVEATGQFD
                     SSRELRGAMNVIADAADTRFAISAAGRDVTVSVYGLGMLMPGMEVPDLPAGEAEAHVA
                     LGVLNDQLMMLDDWLPAGAWATDGWQPYEPEALRLYVRDVTDEPVEGDLPEHVMDWPT
                     DDDPAAFGEEQPFFGNGTRCGIVAGADWLAALSEATQSTRWTDDGERRFTVAPRPLLP
                     HEEPMCPEAGTA"
     gene            3541..4482
                     /locus_tag="VHK28_00645"
     CDS             3541..4482
                     /locus_tag="VHK28_00645"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2140767.1"
                     /translation="MRRSLAAAIGWLLLTPGLVLAHGADAPAPTFPGVLAQWSFDPLA
                     VIGLLLAGGLYLSAVRQVNAAHRSNPHPRWRTWVFLGGLATIGVALLSPIEAYEGALF
                     SVHMVQHMLLQLVAAPLLLAGAPITLALRVASPAVRRHLLAVLHSRVLRALSFPVLAW
                     VLFAATNWGWHYSTLYNDALENQLLHYLQHANFLAVALLFWWPAIGVDPSPWRLPHPV
                     RLFYLFLAMPQQSFLGVSLLQAPTVLYPHYVTNVRDWGMTPLEDQQLGAVIMWVVGDM
                     AFLAGMAVVVLLWMRQEERRTARIDARLAAERAARSD"
BASE COUNT          781 a         1572 c         1593 g          750 t
ORIGIN      
        1 agcagcacga tcgccatcag cgagaacgcc atcatccggt agccgaacac cggtttcctg
       61 gcgttggccg agatcacctc gctgatgatc ccgaagacgg acccgccgaa cagcacgtag
      121 tggatgtgcg cgaccaccca gtacgtgtcg tggatggcga agtcgaccgg gaccgacgcg
      181 ctgaacgcgc cgttgatgcc gccgatcagg aacatgctca ggaagccgag cgcgaagagc
      241 agcggggtgg agagccgcag cttgccccgc cacatcgtga agatccagtt gaacatcttc
      301 acgcccgtcg gaacgccgat cagggcagtc atgaacgcga agaagaggag gaacacctcg
      361 ccggtcgtga acatgtggtg cgcccacacg ctgaagctga gcaggccgat gccgaccgtc
      421 gcgaacacga aggcccggta gccgaacagc ggcttgcggc tgaagaccgg caggatctcg
      481 ctgaccacgc ccattgccgg caggatgacg atgtacacct ccgggtggcc gaagaaccag
      541 aagatgtgct gccacaggat cgggttgccg ccggccgccg ggtcgaagaa cgccccgccg
      601 tagttgcggt cgatgaatag cgcgatcagg cccgcggtga gcaccggggt ggccagcagg
      661 atcagggtgg cggtgaccag caccgtccac acgaagatcg gcatccggaa gagggtcatg
      721 cccggagccc gcattttgaa gacggtggcg atgaagttga tggccccgag gatcgaggcc
      781 gtcccgacca gcgccagccc gatgagccac aggtcggtgc ccacctgagg ggcgtagtcc
      841 tccgtcgaga gcggcgtgta catcgtccag ccctcggccg ccgcgccccc gaacaggtag
      901 ccactgaaaa tcatcaggcc acccagcggc aggatccaga acgacagcgc gttgatgcgc
      961 gggaacgcca tgtccggcgc gccgagctgc agcggcacga tgtagtttcc gaaaccggcg
     1021 aggaccggca cgatgaacag gaagatcatg atcgtcccgt gcatcgtgaa cgcctggttg
     1081 tacgtatccc ggtcgacgaa ctgcaggccg gggacggcca gctccgaacg gatgacgagc
     1141 gccaggatgc cggcggcgaa gaagaacagg aagctgttga cgatgtagag gattccgatc
     1201 ttcttgtggt cgaccgtcgt cagccaatcg atcacccacg atcggccggc ggtgccggcg
     1261 cgcggatcga gcgtggtggt tgccatgggt cttctcgctc cgctactggc tggtgacgat
     1321 gacgtcgccc accatcgtgt cgggatggac gtcgcagatg aattggtact caccggctgg
     1381 cagcgcgggc acctggtagg tgatggtgcc cgcctcgtcc aggatgtcgc cggtgaagag
     1441 ggcctccgtg ccgtcgtaga tgctcacgtt gtgcgggatg tcctcgcggt tctcgaagtt
     1501 gatgcagaag gccgtgtccg ccgggacctc gaactcgctg atatcgaact cgacgttctc
     1561 ggcgccgatg tcgacggtga cggcgcagtc acccgcagca ggctcctgcg gctcttcacc
     1621 ggccgcgagg tccgccaggt aggcgtcgta gtcggcgcgc tccctggcct ccacgacgaa
     1681 ggtcatatcc gcatgttggg tgccgcagaa ctcggcgcat tgaccggcat aggtgccgac
     1741 ctcgtcgacc gtgaagcgca gctcgttcgg ctcccgctgc gggccgaaat cgatcaggtc
     1801 gcgcttgatg aggaagtccg gcacgtagaa cgagtggttc acgtcccgtg tcgtgagggt
     1861 cagcacgacc ggctcgccga ctggcaccac catgacgggc ggatcggcgg ccgtccctcc
     1921 gatggtgacc tccggatcct ggtagtggaa ctgccagctc cactggaagc cgtccacctc
     1981 gatctgaacc ccctcgtctc cctcgacccg agcctccacc tcggccaggc tcagcatgct
     2041 gaaggtgaag agggtgagca cgatgacggt cgggatggcc gtccagatga tctcgatcag
     2101 gttgttgcca tggtgctgct cgggcagcac ctcatcgccc ggcttgcgcc ggtagcggaa
     2161 gaccgcgtac agaatgaagc cctcgacgcc gacgaagacg attgccgcca gcaccagcgt
     2221 gatgaggtac aggttgaaga cgctctcgcc cgcctcggtc ttcggctcgg gcggaagcag
     2281 gcatccaccg agcagcagga tcaggagcag gggtgcgccg acgcggagca tcgtccgcat
     2341 gcgggagcgg ggcgccggga attgcatcag ctcgttctcg aggctcctga cttcgaccga
     2401 cagggtgggg ccacggcgcg agcacgggcc gcgccgatgc ggcacgtcag cattgtagcg
     2461 aaggccgtca tcggggtgac ggccccgcgc gacggcccgg acgcgacctc tccagggcgc
     2521 tgatttcagg ccgttccggc ctccgggcac atcggctctt catgcggcag gagcggccgc
     2581 ggcgccacgg tgaaccggcg ctcaccgtcg tcggtccagc gcgtcgactg tgtcgcttcg
     2641 ctgagggcgg cgagccaatc cgcgcccgct acgattccgc agcgggtgcc gttaccgaag
     2701 aacggctgct cctcgccgaa ggctgcggga tcgtcatcgg tcggccagtc catgacgtgc
     2761 tcgggtaggt caccctcgac cggctcgtcg gtcacgtcgc ggacgtagag ccggagcgct
     2821 tcaggctcgt agggctgcca gccgtccgta gcccaggcac cggccggcag ccaatcatcg
     2881 agcatcatca gctggtcgtt cagcacgccg agcgccacgt gcgcctcggc ctcgccggcc
     2941 ggcaggtccg ggacttccat accgggcatc agcatgccga ggccgtagac gctgacggtc
     3001 acgtcccgac cagccgcgct gatcgcgaag cgggtgtccg ccgcgtcggc gatcacgttc
     3061 atggccccgc ggagctcgcg ggaggagtcg aactgaccgg tcgcctcgac cgcttcgagg
     3121 atggcctgga tcccgtcctc ggtcagccgc cgctcgatca acgccgggag tgcggggccg
     3181 ggaaactcca gcgtctgcat ccccatcatc accacgcggc catcgccgaa gagtgagaag
     3241 ctcggcatct gcgtcacgag gaagtcgacc gggacgaagc cgccgaccgc gtcgaccacc
     3301 aggatggcct cgtcggcgcc gatcgggtgc tcgatgccgc cgccacctcc gtcatcggtg
     3361 tcgggtgccg acggatcggc cgacacctcc gccccgcccg gcgcaccgcc ggaggcgcag
     3421 gccgagagag cgaggagcac gacgagcagc gtggccattg acggccggag gtggttcatg
     3481 gcgcctgtga cgccggatgc ctcccggcgg ttccatcgga ttccggccac aatgggactg
     3541 atgcgtcgct cgctcgcggc cgccatcggc tggctgctcc tgacccccgg cctcgtcctg
     3601 gcccatggcg ccgatgcgcc cgctccgacc ttcccgggcg tgctggcgca gtggagcttc
     3661 gatccgctgg ccgtgatcgg cctcctcctg gccggtgggc tgtatctctc agccgtcagg
     3721 caggtgaacg cggcccaccg gtccaacccc cacccgcgct ggcgcacgtg ggtgttcctc
     3781 ggcgggctgg cgaccatcgg cgttgcgctc ctgtcgccga tcgaggcgta cgagggcgcg
     3841 ctgttcagcg tgcacatggt ccagcacatg ctcctgcagc tggttgccgc gccgttgctg
     3901 ctggctggag cgccgatcac actcgccctg cgggtcgcct cacccgcggt gcggcggcac
     3961 ctcctggctg tcctccacag ccgcgtgctg cgcgcgctct ccttcccggt cctcgcgtgg
     4021 gtcctcttcg cggccacgaa ctggggctgg cactacagca cgctctacaa cgatgcgctc
     4081 gagaaccagc tgctccatta cctgcagcac gccaatttcc tcgccgtggc gctgctcttc
     4141 tggtggccgg cgatcggtgt cgatccgtct ccgtggcggc tcccccaccc ggtgcgtctg
     4201 ttctacctgt ttctggccat gccgcagcag tcgttcctcg gcgtgtcgct gctccaggca
     4261 ccgacagtcc tctacccgca ttacgtcacc aatgtccgcg actgggggat gacgccgctc
     4321 gaggatcagc agctcggagc ggtcatcatg tgggtcgtcg gtgacatggc cttcctggcc
     4381 ggcatggccg tggtcgtgct gctctggatg cgccaggagg agcggcgcac tgcacgcatc
     4441 gacgctcgcc tggccgcgga gcgtgccgct cgcagcgact aggcccgtac gccctcgtgg
     4501 agccgcaagg gatgcgggat tggaagtcgg gtggccgccg ctcggtggct aggccagcgg
     4561 gaagcggaag acggcgtcag cagccaccgc cgcaaacagg agggtcaggt agctgatcga
     4621 gtagcggaac agcgagatcg cctcgcgtcc ctggccgcgc gcacgctgga gccggagcgc
     4681 gccgaggacg tagaag
//
LOCUS       DATACA010000030         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1128267, whole genome shotgun sequence.
ACCESSION   DATACA010000030 DATACA010000000
VERSION     DATACA010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128267"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VHK28_00650"
     CDS             complement(<1..1175)
                     /locus_tag="VHK28_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2140768.1"
                     /translation="MTTTGRPGAGEGGLSPQIRVLPRGERRLLAHLGELDPDSIASYR
                     EHGGYAGLERAVQHLGPEAVIEEVASANLRGRGGGGYPTAEKWRSARAAESTRKIVVA
                     NLMGADPTALGDRALAEANPHLVLEGVLIAAFATGASEAILAVRRDWPLAISRLKAAI
                     EQAEAAHLAGYLVMGTDFSSTVTVREGSGALVAGEESALLASLAGDRGMPVIRPPYPT
                     EVGLWGAPTIVQNAETLAHAAWIVAHSAEAYGTVGTASAPGTRLVSIYGKVGEPGVVE
                     VPLGTPLSEVISLAGGSVGVTKAVIVGGPAGGALSAGELSTPYEYDALRAAGGGIGLG
                     SMLVLDTETCMVATARFFLDWSAREACGKAVPCRIGTKRLVEALDRVLAATPRPNDFV
                     "
     gene            complement(1179..2027)
                     /locus_tag="VHK28_00655"
     CDS             complement(1179..2027)
                     /locus_tag="VHK28_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX2140769.1"
                     /translation="MAIPGAGIAQGMWTTLRNLFEPKVTRQYPDERPQMPERWRGRLD
                     LIYDPFGEHKCEVCFQCAMVCPVEAIDMSGFDSQGNRIRYGMPEIYDERRDPNAYRRA
                     GLPARPMRNAARWEAEVDEPWVAEVVDGYRGRPEALVAIFGAVQERYGFLPEPALRLI
                     SSRMTIHWAQVFGAAGLGGFRLLPAEGHVITVCTCPACHFSGGRELLAAVSAELGIAP
                     GETTDDGAFTLETSPDVGAGAIAPAMRIDTLVYGPLDAEKARHLVEQRRRTGQPAGAT
                     PVVATS"
BASE COUNT          292 a          786 c          770 g          296 t
ORIGIN      
        1 acgaagtcgt tcgggcgcgg ggtggcggcc agcacccggt ccaacgcctc caccaggcgc
       61 ttggtgccga tccggcaggg gaccgccttg ccgcaggcct cgcgggcaga ccagtcgagg
      121 aagaagcgcg ccgtggcaac catgcaggtc tcggtgtcga gcaccagcat ggatcccaga
      181 ccgatgcctc cgccggctgc ccggagcgcg tcgtactcgt agggcgtcga cagctccccg
      241 gccgagaggg ccccgcccgc cggtccgccg acgatgaccg ccttggtcac gcccacggat
      301 ccgccggcca ggctgatgac ctcgctcagc ggcgtgccga gcggtacctc gacaaccccc
      361 ggctcgccca ccttgccgta gatgctgacc agccgagtgc cgggcgccga cgctgtcccg
      421 accgtgccat aggcctccgc ggagtgggcc acgatccagg ccgcgtgggc cagcgtctcc
      481 gcgttctgca cgatggtcgg ggcgccccac agcccgacct cggtggggta cggcgggcgg
      541 atgaccggca tgccgcgatc gccggccagg gaggccagca gcgccgactc ttcgccggcc
      601 accagcgccc cggatccctc gcggacggtg acggtggagc tgaagtcggt gcccatcacc
      661 aggtacccgg ccaggtgcgc ggcctcggcc tgctcgatgg cggccttgag gcggctgatc
      721 gccagcggcc agtcgcggcg cacagccagg atcgcctcgg acgcgccggt ggcgaaggcg
      781 gcgatcaaca cgccctccag caccaggtgc gggttcgcct cggccagcgc cctgtcgccg
      841 agggcggtgg ggtcggcgcc catcaggttg gcgaccacga tcttgcgcgt gctctccgcc
      901 gcgcgggcgg accgccactt ctcggcggtg ggatagccgc cgcctcctct gccgcggagg
      961 ttggcgctgg ccacctcctc gatgaccgcc tccgggccca ggtgctgaac cgcgcgctcc
     1021 aggccggcgt agccgccgtg ctcgcggtag gacgcgatgc tgtcgggatc cagctcgccc
     1081 aggtgcgcca gcagccgccg ctcgccgcgg ggcaggacac ggatctgggg cgagagcccg
     1141 ccttcgccgg ccccgggccg tcccgtggtg gtcatggatc agctggtggc cacgaccggc
     1201 gtggcaccgg ccggctggcc ggtccgccgg cgctgctcga cgaggtgccg cgccttctcg
     1261 gcgtccagcg ggccgtatac cagggtgtcg atcctcatgg cgggggcgat agcgccggcg
     1321 cccacgtccg ggctggtctc cagggtgaac gcgccgtcat cggtcgtctc gcctggcgcg
     1381 atgcccagct cggcgctgac cgcggccagc agctcgcgtc cgcccgagaa gtggcaggcc
     1441 gggcaggtgc agacggtgat gacgtgcccc tcggcaggca gcagtcggaa gccgcccaag
     1501 ccggctgccc cgaagacctg cgcccagtgg atcgtcatgc ggctgctgat gaggcgcagg
     1561 gccggttccg gcaggaagcc gtagcgctcc tggaccgcac cgaagatggc caccagcgcc
     1621 tccgggcggc cgcggtagcc atccaccacc tccgccaccc acggctcgtc cacctccgcc
     1681 tcccagcgag cggcgttgcg catcggtcgg gcgggcaggc cggcgcgccg gtaggcgttc
     1741 ggatcgcggc gctcgtcgta gatctcgggc atcccgtagc ggatgcggtt gccctgggag
     1801 tcgaagccgc tcatgtcgat ggcctccacc gggcagacca tggcgcactg gaagcagacc
     1861 tcgcacttgt gctccccgaa ggggtcgtag atcaggtcca gccgccctcg ccagcgctcc
     1921 ggcatctgcg ggcgctcgtc ggggtactgg cgggtgacct tgggctcgaa caggttgcgc
     1981 agggtggtcc acatgccctg ggcgataccg gcgccgggga ttgccatgag tccttacgcg
     2041 cctcccaccg gttgcaggtt gaagaacaga acgatggccg cggtgacgaa caggttgacc
     2101 agcgccagcg gcagcagcca cttccaggcc atgcccatca gctg
//
LOCUS       DATACA010000031         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1530092, whole genome shotgun sequence.
ACCESSION   DATACA010000031 DATACA010000000
VERSION     DATACA010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530092"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VHK28_00660"
     CDS             <1..543
                     /locus_tag="VHK28_00660"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HEX2140770.1"
                     /translation="VFDEADPPPAGLRQHGEVMVAWRVTMFKKIKYHTHENVGWGSIS
                     IPETEMHTTATWLVPPAEVVNRYDRDTLDGALIGLARVARTTAALLLMSDPRDLGVLA
                     QVQAPFTGRPTLYLYDAVPGGVGLTERLFTLVDDLLVACRGVVESCACADGCPACVGP
                     AIEVGQRGKAVAAELLAAMT"
     gene            547..1809
                     /locus_tag="VHK28_00665"
     CDS             547..1809
                     /locus_tag="VHK28_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX2140771.1"
                     /translation="MLQQRSLADRLAAIRAGHAPAVASRPALPDRAESLARWFSARLA
                     VAEGGAAVVVERGVALSPPVAARLATLPPASYFDTETTGLSTGAGTVVFLSGTGWLDG
                     SRLVVRQLLLPDYPHERALLRLLGAHLAERPRLVTYNGRGFDMPLLIARLTVNGLFRE
                     QAALPERHDDLLPVARRLFRRPLGGARLADVETGVLGVHRASDCPGSEVPMRYFGYLR
                     GGSPDLLAEVLDHNLQDIVSLALLESEVLRLRAGGWRDATVLDRRGMAVELMRDGAVD
                     DALALLEEASFSVSDPGEVTSLRRLATRLMLAAGELDRAEALWTAGTRRASLDAAAAW
                     IEVARIRERHRGDLQGALEAAAAASRVLDLAFALGRGGSLAEVGRIRLRVESRLRRLR
                     TWTQAAERRRARATRRAEPQLKVAAGSR"
     gene            complement(1779..>2568)
                     /locus_tag="VHK28_00670"
     CDS             complement(1779..>2568)
                     /locus_tag="VHK28_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2140772.1"
                     /translation="SGRAAYVLGDVHGMLEPMRVLLRDAGLTDAEDRWSGGRHELWLT
                     GDLVDRGTAGVEVIAMLMRLQHEAASTGGRVASVAGNHELLLLAAREIPNEVAGGPGG
                     TFLRDWLANGGQHSDLDALTPEHTSWLRRLPAVARVGTVLLVHADAMLYASLGASVSE
                     VNERLTEWLTTADPDRWDELLAGLSARRAFLADPKAADRLLARFGGRQLVHGHTPIPH
                     LVRGEARRVSVPHLYADGRVVAVDAGLAAGAAGFLYRLPAATFS"
BASE COUNT          341 a          932 c          937 g          358 t
ORIGIN      
        1 gtcttcgacg aggcggatcc gcctccggcc gggctccggc agcacggcga ggtgatggtg
       61 gcctggcggg tgaccatgtt caagaagatc aagtaccaca cgcacgaaaa cgtcggctgg
      121 ggctccatca gcatccccga aacggagatg cacaccaccg ccacctggct ggtgccgccg
      181 gcagaggtcg tcaaccgata cgatagggac acgctcgacg gcgcgctgat cgggctggcc
      241 cgcgtggcac gcaccaccgc tgccctgctg ctgatgtccg atccccgcga cctgggcgtg
      301 ctcgcccagg tgcaggcgcc gttcaccggg cggccgacgc tgtacctata cgatgccgtg
      361 cccggcggcg tcgggctgac cgagcggctg ttcacgctcg tggacgacct gctggtggcg
      421 tgccgcggag tcgtggagtc ctgtgcctgc gcggatggct gcccggcctg cgtgggcccc
      481 gccatcgagg tcggccagcg cggcaaggca gtcgccgccg agctgctggc ggccatgacc
      541 tgagccatgc tgcagcaacg atccctggcg gaccggctgg cggccatccg tgccggacac
      601 gcgcccgccg ttgccagccg cccggcgctg ccggaccgcg cggagtccct ggccaggtgg
      661 ttcagtgctc gcctggcggt cgcggagggc ggcgccgcgg tagtggtgga gcggggcgtg
      721 gcactgagcc cgccggtggc cgctcggctg gccaccttgc cgccggccag ctacttcgac
      781 accgagacga ccggcctgtc gacgggcgcc ggcacggtcg tgttcctgtc cgggaccggc
      841 tggctggacg gctcgcggtt ggtcgtccgc cagctgctgc tgcctgacta cccgcacgag
      901 cgggcgctgc tccgcctgct cggagcccac ctggccgagc ggccccgcct ggtgacctac
      961 aacgggcgcg gcttcgacat gccgctgctg atcgcgcgcc tcacggtcaa cggcctgttc
     1021 cgcgagcagg cggcgctccc ggagcgtcac gacgatctcc tgccggtcgc cagacgcctc
     1081 ttccgccgcc cgctcggcgg cgcccggctg gcggacgtgg agaccggcgt gctcggcgtg
     1141 caccgtgctt ctgactgccc cgggagcgag gtgcccatgc gctacttcgg ctacctccgc
     1201 ggcggctcgc ccgacctgct ggcagaggtg ctcgatcaca acctgcagga catcgtgtcg
     1261 ctcgccctcc tggagtcgga ggttctccgg ctgcgggccg ggggctggcg ggacgccacg
     1321 gtgctcgacc ggcgtggcat ggccgtcgag ctgatgcgcg atggagccgt ggacgatgcg
     1381 ctggcgctgc tggaggaggc gagcttctcg gtgagcgatc ccggcgaggt cacgtcgctg
     1441 cgtcgcctgg caacgcggct gatgctggcg gccggggagc tggaccgtgc cgaggcgctg
     1501 tggaccgccg gcacccgccg cgcgtcactg gatgccgccg cggcctggat cgaggtggca
     1561 cgcatccgcg aacgccaccg cggagacctg cagggtgcgc tggaggcggc ggcggccgca
     1621 tcgcgggtcc tggacctggc cttcgccttg gggcgcggcg gcagcctggc cgaggtgggg
     1681 cgcatccgcc tccgcgtcga gtcgcggctc cggcgcctga ggacctggac acaggcggcc
     1741 gagcggcgtc gcgcccgggc cacccggcgt gccgaacctc agctgaaggt ggccgccggc
     1801 agccggtaga gaaagccggc agcgccagcg gccaggccgg cgtctaccgc caccactcgg
     1861 ccgtcagcgt agaggtgcgg aacactcacc cggcgtgcct cgccgcggac caggtgcggg
     1921 atcggtgtat gcccgtgcac aagctgtcga ccgccgaatc gtgccagcag ccggtcagcg
     1981 gctttcgggt cggcaaggaa ggcgcgtcgc gcgctcaaac cggccagcag ctcgtcccag
     2041 cgatcgggat cagcagtcgt cagccactcg gtcaagcgct cattcacctc cgagacggac
     2101 gcgcccaggc tggcgtacag catggcgtcg gcatggacca gcaggacggt ccccacgcgc
     2161 gcgacggcgg gcagtcgccg cagccaactg gtgtgttcgg gagtgagggc gtccaggtcc
     2221 gaatgctgcc cgccattggc cagccagtcc ctcaggaacg tgccgcccgg gccgccggcc
     2281 acctcgtttg ggatctcgcg agcggcgagc agcagcagct cgtggttgcc ggccaccgac
     2341 gccacccggc cacccgtcga cgccgcctcg tgttgcaggc gcatgagcat ggcgatcacc
     2401 tcgacgccgg cggtgccccg atccaccaag tccccggtca gccacagctc gtggcggccg
     2461 ccgctccacc ggtcctcggc atcggtcaaa cccgcgtcgc gaagcagaac gcgcatcggc
     2521 tcgagcatgc cgtgcacgtc gccgaggacg tacgccgcgc gacccgag
//
LOCUS       DATACA010000032         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1449716, whole genome shotgun sequence.
ACCESSION   DATACA010000032 DATACA010000000
VERSION     DATACA010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449716"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VHK28_00675"
     CDS             <1..1395
                     /locus_tag="VHK28_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX2140773.1"
                     /translation="SRPGPWTSSRTRQIRSTPMRSSWAVRVLLPATQVIAEGAWLAVF
                     YAAAQAIIGETPWIGPLELAVAAWAGMAWGRRRRWTGAGAEAIGLPLLALAAGGVGWL
                     LDPGVRLLLAQGEPMAALGTHAPGWLAALAFWRGEVHRSVEDDDAHADQLLRWMVPGL
                     ALPWIAGHLAASGRVEDDFVAAAFVGTVFFVAAAFLAMGLARLEAVRASTGSNWQASR
                     SWIVLVVGIALGVTVLAIPAAAFLGVPIRSLLVALLGPLQTILFALVLLATPIILLAA
                     AVADLVQPFLPEGFGLGQIDLPSLAPQRQPIGSSVPTWVFFIIVGLLLIVELAVLGAI
                     IWMRWQERRRMRVAQTDDFEERAIVIPQPDPPRTAAPRTARRRQLPLDHPAGAYVAAL
                     HELDRDGRWPRQPFETPRNHAARARDEGLGTGALARLAAAYQLWRYGGRDLGSAEGRR
                     GRGRLAALRRVLRD"
BASE COUNT          224 a          652 c          656 g          299 t
ORIGIN      
        1 tcccggcctg gaccgtggac gtccagccgg acccgtcaga tccggagcac gccgatgcgc
       61 tcgtcctggg ccgttagggt cctcctcccc gcgacccagg tcatcgccga gggtgcctgg
      121 ctggcggtct tttacgccgc ggcgcaggcg atcatcgggg agacgccctg gatcgggccg
      181 ctggagctgg cggtggcggc ctgggcgggc atggcctggg gccgccgcag gcgctggacc
      241 ggcgccggcg cggaggccat cggcctgccg ctgctggccc tggcggccgg cggcgtgggc
      301 tggctgctgg accccggggt gcgcctgctg ctggcgcagg gagagccgat ggccgcgctg
      361 ggcacccacg cccccggctg gctggcggcc cttgccttct ggcgcgggga ggtgcaccgc
      421 tcggtggagg acgacgacgc ccacgccgac cagctcctgc gctggatggt ccccggcctg
      481 gccctgccgt ggatcgcggg ccacctggca gcgagcggcc gcgtggagga cgacttcgtg
      541 gcggccgcct tcgtgggcac cgtcttcttc gtggcggccg ccttcctggc catgggcctg
      601 gcacggcttg aggcggtgcg cgcctcgacc ggctccaact ggcaggcgag ccgctcctgg
      661 atcgtgctgg tggtgggcat cgccctgggc gtcaccgtgc tggccatccc ggccgccgcc
      721 ttccttggcg tgccgatccg cagcctgctg gtggccctgc tgggcccgct gcagaccatc
      781 ctgttcgcgc tcgtcctgct ggccacgccc atcatcctgc tggccgccgc ggtggccgac
      841 ctggtgcagc ccttcctgcc cgagggcttc ggcctggggc agatcgacct gccgtcgctg
      901 gcgccgcagc ggcagcccat cggctccagc gtgccgacct gggtcttctt catcatcgtc
      961 ggcctgctgc tgatcgttga gctcgcggtg ctgggggcca tcatctggat gcgctggcag
     1021 gagcgccgac ggatgcgagt cgcccagacc gatgacttcg aggagcgcgc catcgtcatc
     1081 ccgcaacctg atccgccgcg gacagcggcg ccacgcacgg cccgacgtcg ccagctgccg
     1141 ctcgaccacc ccgccggtgc ctacgtggcc gcgctccacg agctggatcg cgacggacgc
     1201 tggccgcgac agcccttcga gacgccgcgc aaccacgccg cccgcgcccg ggacgagggg
     1261 ctcggaaccg gcgccctcgc ccggctggcc gccgcctacc agctgtggcg ctacggggga
     1321 cgcgacctcg gttctgcgga gggccgccgc ggccgggggc gcctggcggc cctgcgccgc
     1381 gtgcttcggg actgatctgc cgctcatgaa tcaggcagga attcacctga ggtgcgtgcg
     1441 gcgtgctgtt ctgccggtac agcgggcaga ggtgagcctg ggaggcgaag ttcttgtccg
     1501 ttcctgcgcg cgcctgcccg atgtagcggc gaatcgacac gcagcatcgg tcccgcgtga
     1561 aaaattgccc gccatgcccg ggcgccatcg gtttagatag ataccttgac aacatcattg
     1621 tcagggttta gggtaaccct gcgttcctgg ttcacagtgg tgaaggaggt tcgaacagga
     1681 ggaaatggct aacatcacgc gattcgaccc attcaacgag atgatcaccc tgcggcaggc
     1741 catggaccgg ctgttcgagg attcgttcgt gagcccgctc accatgcggt ccttcaacgg
     1801 cgaggcgtcc gcgccggcac tggacgtgca c
//
LOCUS       DATACA010000033         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2816150, whole genome shotgun sequence.
ACCESSION   DATACA010000033 DATACA010000000
VERSION     DATACA010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816150"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..727
                     /locus_tag="VHK28_00680"
                     /pseudo
     CDS             224..727
                     /locus_tag="VHK28_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            1036..1506
                     /locus_tag="VHK28_00685"
     CDS             1036..1506
                     /locus_tag="VHK28_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2140774.1"
                     /translation="MRVACEAARLVAAGGIPVNGPLVEAAALLHDIDKPETRAGVGEH
                     GVVGAARMIERGWEELALPIASHPVTALLDDDRHPKGWESVIVSVADRHVAQEFLTTD
                     ERIDDLVARHPQHSSSLEAARRPAAALEREVADAAGLEVDELVSRLRAAWEAGA"
     gene            1503..2543
                     /locus_tag="VHK28_00690"
     CDS             1503..2543
                     /locus_tag="VHK28_00690"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140775.1"
                     /translation="MSIPLLLAHGDDSYGLDQVLAAFAKRVGADDRVEIVAERSPDEG
                     SIDRARVEAGTIGMFGVHLAVLRQPVRAAGRSTAVGDKLMSLVRDLPDGAALALVDLR
                     ASRDAAKPPALVQRLADAVITRGGIVEERLAPRRGELVAWIKRHADALGTRIDGRAAM
                     LLAERVGGAVAETDIERGEQTRVTDSELRKLATYAGERPIEVADVEALVADTRPASIY
                     AITNAVDRREPAAASEAVRRALAEAQPVLRIMAALSGRVSDLIVARDLAARRVAPAEI
                     TKRIGRGNARMAERLVEAARRYEGAELEAMLRGLFEADLAIKSNAMEPEPALVAWIGE
                     FLLGTRRSAAAR"
     gene            complement(2754..3014)
                     /locus_tag="VHK28_00695"
     CDS             complement(2754..3014)
                     /locus_tag="VHK28_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140776.1"
                     /translation="MSEPIYASRAPETCRFCSGELLSSVFDTAFRDGAADERLFFAIP
                     GALCVPCRQLYVDQQLIGILGLADARCTFAIESDRVLRPNAA"
     gene            complement(3101..3475)
                     /gene="rsfS"
                     /locus_tag="VHK28_00700"
     CDS             complement(3101..3475)
                     /gene="rsfS"
                     /locus_tag="VHK28_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX2140777.1"
                     /translation="MAEPAGQHTASDPAELAHKIVDVASDKKGVDIVLLRTSEVTTMA
                     DYFVITSGRSERQVQALSTAIVDELRDDGIRPIGVEGMSGAKWVLLDYGSVIVHVFAP
                     EEREYYGLERLWSNAAQVVRMV"
     gene            complement(3502..>4109)
                     /gene="nadD"
                     /locus_tag="VHK28_00705"
     CDS             complement(3502..>4109)
                     /gene="nadD"
                     /locus_tag="VHK28_00705"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2140778.1"
                     /translation="EPRRLGILGGTFDPPHVGHLWLATLAADVLGLDRVLFMPAAQPP
                     HKRGRLMSSAGDRLLMTRLAIAADETFELCPIEMERPGPSFTIDSVQELRRLHQGAEL
                     FLVMAADSLAQIDTWREPDRLLSLVEWAVGPRPGSPLPERALLERRFGAASSRIHLLE
                     GPSLAVSASEIRRRVAAGRTIRYLVPRAVEELIIERRLYRP"
BASE COUNT          589 a         1368 c         1581 g          571 t
ORIGIN      
        1 cgcgcgacgc gcggggggta ggaccgatgc caccgcggat gccactccca tcgacaacat
       61 ggtggtgcgg ctggcacgtc cacgcctggt gctgggcgcc accctggcgc tgctggtggc
      121 ggtcaccgtc gccacccggc ccgacgggcg gctgcacctg ttcgccctgg acgtggggca
      181 gggtgacgcc atcgtggtgg tggcgcccgg cggcgaggtg gccatgatcg acggtggccc
      241 ggatcccgac gtggccatgc gccggctggg cgaggtgctg cccttctgga agcgacgcat
      301 cgacgtgctg gtgctgaccc acccgcacga ggaccacgtg gccgggctgc tgccggcact
      361 cgagcgctac gaggttggca ccgtgctgga ttcggggcgc gagtacgaca acccgtccta
      421 cccccggttc ctgaagctgg catcccgcga gcccgatgcg ctggttcgcc acgcacgcgc
      481 cggcgacatc ctccagctgg gcgaggacac ccggctgacc gtcctcttcc cctccgggac
      541 cgatgcggac gcgccacttc ccgagggaga catcaacaac gcgtcggtgg tgctcctgct
      601 ggagcacggc agcttccgcg cgctgctgac gggcgatgcc gaggcgccgg tggagcaggc
      661 gctgctggcg cgcgggctca tcggtccggt ggacgtgctc aagatcggac accatggatc
      721 ggactcgtcc accacggccg gcctggtggc cgcggcccgg ccgggggtcg ccctgatctc
      781 ggccggcgtg gacaacgagt acgggcaccc gcacgccgtc acgctggagc agttggccgg
      841 ggtgcaggtg catcggaccg acctggaggg cagcctcgag gtggtggtgg acgcatttgg
      901 ctaccgggtg gaggcccgcg gtgggagcga tgcgggtagc atcggtccat ggccgtacca
      961 acccgggagc aggccgagcg cgtcctggag gaggcgggcc tgccggcggg catcgtcatc
     1021 cattcgcatg gagtcatgcg cgtggcctgc gaggcggcac gactggtggc cgcaggtggg
     1081 atcccggtca acggtcccct ggtggaggcc gccgccctgc tgcacgatat cgacaagccg
     1141 gagacgcgcg ccggcgtcgg cgagcacggc gtcgtcggag cggcgcgcat gatcgagcgc
     1201 gggtgggagg agctggccct gccaatcgcg tcgcacccgg tcaccgccct gctggacgac
     1261 gaccgccatc cgaagggctg ggagtcggtg atcgtgtcgg tggcggaccg gcacgtggcg
     1321 caggagttcc tgacgaccga tgagcgcatc gacgacctgg tcgcgcgcca cccccagcac
     1381 agttcctcgc tggaagcggc gcggcggccg gcggcggcac tcgagcgcga ggtggccgat
     1441 gcggccgggc tggaggtgga cgagctggtg tcgcgcctgc gggccgcgtg ggaggcgggc
     1501 gcgtgagcat cccgctgctg ctggcccacg gcgacgactc gtacgggctg gaccaggtcc
     1561 tggcggcctt cgcaaaacgg gtgggggctg acgaccgggt ggagatcgtg gccgaacgct
     1621 cgccggacga gggatcaatc gaccgcgcgc gggtggaggc cggcacgatc gggatgttcg
     1681 gcgtgcacct ggccgtgctg cgccagccgg tgcgggcggc gggacgctcg acggcagttg
     1741 gcgacaagct gatgtcgctg gtgcgagacc tgcccgatgg cgcagccctg gccctggtcg
     1801 acctcagggc atcccgcgac gcggccaagc caccggccct ggtgcagcgc ctggcggatg
     1861 cagtgatcac ccgcggcggg atcgtggagg agcggctggc acctcggcgc ggcgagctgg
     1921 tggcctggat caagcgccac gcggacgcgc tcgggaccag gatcgacggt cgcgccgcca
     1981 tgctgctggc ggagcgggtg ggaggcgccg tggccgagac ggacatcgag cgcggagagc
     2041 agacccgggt gacggacagc gagctgcgca agctggcgac ctacgccgga gagcggccga
     2101 tcgaggtggc cgatgtggag gcgctggtgg cggacacccg cccggccagc atctatgcca
     2161 tcaccaacgc ggtcgaccgc cgggagccgg ccgccgcctc cgaggcggtg cgtcgcgcgc
     2221 tggccgaggc ccagccggtg ctgcgcatca tggccgccct gtccggcagg gtgagcgacc
     2281 tgatcgtggc gcgcgacctg gccgcccgac gggtggcgcc cgcggagatc accaagcgga
     2341 tcgggcgggg caatgcccga atggcggagc ggctggtgga ggcagcccgg cgctacgagg
     2401 gcgccgagct ggaggccatg ctgcgtggcc tgttcgaggc ggacctggcg atcaagtcca
     2461 atgcgatgga gccggagccg gcactggtcg cctggatcgg cgagttcctg ctgggcactc
     2521 gtcggtcagc cgcagctcgg taggtcgggc ggtcgcgcgc tggagcgcgt cacggtgcgc
     2581 ggccacgggg tggctctaac ggccatccag gcggtcggat tagccgcatc ttggccgggg
     2641 tggccacccg atgggcggca cagccaacgg gaagcgtccg tgcgtggctt cttggccgta
     2701 gtggccatct cgtggctacc gaccgccggc gcacgcgcca gccggccggg aagtcaggcg
     2761 gcgttggggc ggaggacccg gtcgctctcg atggcgaagg tgcagcgggc gtcggcgagt
     2821 cccaggatgc cgatcagttg ctggtcgacg tagagctggc ggcacggcac gcacagcgcg
     2881 ccggggatgg cgaagaagag ccgctcgtcg gcggcaccgt cgcggaaggc ggtgtcgaag
     2941 acgctgctca gcagctcccc gctgcagaag cggcaggtct ccggcgcgcg cgaggcgtag
     3001 atgggctcgc tcatgcatgg gagcatgggg tggaccgata ccgccctcaa taggagtacc
     3061 ggcgtggccg gccctagtac tcgtgcagta cccgctgcct ctaaaccatg cgcacgacct
     3121 gggcggcgtt gctccagagc cgctccaggc cgtagtactc gcgctcctcg ggggcgaaga
     3181 cgtggacgat gacgctgccg tagtcgagca gcacccactt ggcaccgctc atgccctcca
     3241 cgccgatcgg acggatgccg tcgtcgcgca gctcatccac gatggccgtg ctgagggcct
     3301 gcacctggcg ctcgctgcgc ccggaggtga tcacgaagta gtccgccatg gtggtcacct
     3361 cggaggtgcg cagcaggacg atgtccacgc ccttcttgtc cgaggcgacg tcgacgatct
     3421 tgtgggccag ctcggcgggg tcgcttgccg tgtgctgccc ggcgggttcg gccaggtgtt
     3481 ctctccccgt gttgcggttg ctcagggccg gtacaggcgc cgctcgatga tcagctcctc
     3541 caccgcccga gggacaaggt accggatggt ccggccggcc gccaccctgc gacgtatctc
     3601 ggaggcactc accgccagcg acgggccctc cagcaggtgg atgcgagagg aggcggcccc
     3661 gaagcggcgc tcgagcaggg cacgctcggg gagcgggctg ccgggccgcg ggccgaccgc
     3721 ccactccacc aatgaaagga ggcgatccgg ctcgcgccag gtgtcgatct gggccagcga
     3781 gtcggctgcc atcaccagga acagctcggc gccctggtgc agacggcgca gctcctgcac
     3841 gctgtcaatg gtgaacgaag ggcccggccg ctccatctcg atggggcaca gctcgaaggt
     3901 ctcatcggcc gcgattgcca ggcgcgtcat cagcagccgg tctcccgcgg agctcatgag
     3961 cctgccccgc ttgtgcggcg gctgggcggc cggcatgaac agcacgcggt ccaggcccag
     4021 cacgtcggcc gccagggtcg ccagccacaa atgcccgaca tgaggtgggt cgaaggtgcc
     4081 gcccaggatg ccgagacgtc tcggttcgc
//
LOCUS       DATACA010000034         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_968750, whole genome shotgun sequence.
ACCESSION   DATACA010000034 DATACA010000000
VERSION     DATACA010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968750"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VHK28_00710"
     CDS             complement(<1..319)
                     /locus_tag="VHK28_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2140779.1"
                     /translation="MEGGAMGGLKSAVLDGSQMAMGELVEHGMAWAMNGIAGLAQEPL
                     VAVERGRTVALTIDNRTAWNHALHLHGHHVRVVEDNGGNGAVPVWRDTVPIEPQATVT
                     LAFI"
     gene            complement(328..1350)
                     /locus_tag="VHK28_00715"
     CDS             complement(328..1350)
                     /locus_tag="VHK28_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX2140780.1"
                     /translation="MWTRRQFLGAAAGLAAFAGGPRLVLGATGGDGFRVLRAKGGVAP
                     LMGDEGDPTAIWGFNGKCPGPTLKVRQGEELKVRLVNELLEPTSIHWHGIRLPNAMDG
                     TDLTQEPVPPGGSFDYVFTPPDAGTFWYHAHVGSAHQVDRGLYGALVVEEETPPEGMS
                     DVLMVIDDWWLLDSGAINEETFGDLMVAAHGGRMGNWLTVNGVSRPHLTAAVGERLRV
                     RLINAANARLMRLRFNAAAPHLLAMDGQPLASPRLLDKDPLVLPPGGRADIGLPRGTE
                     NVAVTIDIGGEALELALIERQDVPEAEPEGKTRATPSRKWIPGISPHSPPIPCPRRST
                     SPRPWR"
     gene            complement(1359..>1739)
                     /locus_tag="VHK28_00720"
     CDS             complement(1359..>1739)
                     /locus_tag="VHK28_00720"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2140781.1"
                     /translation="PQSINVLGGFRTQGRHRDQWAAIEEDARAVSDAGAFAVVLEAMA
                     EPLAATITKAIPIPTIGIGASAVCDGQILVMEDMLGLNPRPPKFVKTFGEVSKAIEAA
                     ISGYAEEVRARSFPALDHTYEVKD"
BASE COUNT          253 a          624 c          585 g          277 t
ORIGIN      
        1 caatgaacgc gagcgtgacg gtcgcctgcg gctcgatcgg caccgtatcg cgccagaccg
       61 gaacggcgcc attgccgcca ttgtcctcca ccacccgcac atggtgcccg tgcaggtgca
      121 gggcatggtt ccaggcggtc ctgttgtcga tggtcagggc gacggtccgg ccccgctcca
      181 ccgcgaccag cggctcctgg gcaagaccgg cgatgccgtt catggcccag gccatgccgt
      241 gttcgacaag ctcgcccatc gccatctggc tgccgtccag gacagcactc ttcaggccgc
      301 ccatggcgcc gccctccatt accagcttca ccgccagggc ctcggagagg tcgagcgcct
      361 cgggcagggg attggcgggg agtggggcga aatccccggt atccacttcc ggctgggcgt
      421 cgcccgtgtc ttcccctcgg gttcggcttc gggcacgtcc tggcgctcga tcagagccaa
      481 ctcgagggcc tcgccgccga tatcgatggt gacggcgacg ttctcggtcc cacgcggcag
      541 gccgatgtca gcgcgtcctc cgggcggcag caccagcggg tccttgtcga gcaggcgcgg
      601 cgaagccagc ggctggccgt ccatggccag caggtgcgga gcggcggcgt tgaaacgcag
      661 ccgcatgagc cgggcattgg cggcattgat cagcctgacc cgcagccgct cgcccaccgc
      721 cgcggtcaga tgcggccggg ataccccgtt gacggtcagc cagttgccca tccgcccgcc
      781 gtgggctgcg accatcaggt cgccgaatgt ttcctcgttg atggcgcccg agtcgagcag
      841 ccaccagtcg tcgatcacca tgaggacgtc gctcatcccc tcgggcggcg tctcctcctc
      901 cacgaccagg gcgccataga gtccgcggtc gacctggtgg gcggagccca cgtgggcgtg
      961 ataccagaag gtgccggcgt ccggcggcgt gaacacgtaa tcgaagctgc cgcccggcgg
     1021 caccggctcc tgggtgagat cggtgccgtc catggcgttg ggcagccgga tgccatgcca
     1081 gtgaatcgac gtcggctcga gcagctcatt gaccaggcgg accttcagct cctcgccctg
     1141 tcgcaccttc agcgtcggcc cggggcactt cccgttgaac ccccagatcg ccgtcgggtc
     1201 gccctcatcg cccatcagcg gcgccactcc gcccttggcg cgcagcaccc tgaagccgtc
     1261 gccgccggtg gcgcccagga ccagccgggg gccgccggcg aacgcggcca gccccgccgc
     1321 tgctccgagg aattgcctgc gggtccacac gggcagcgct agtccttcac ctcgtaggtg
     1381 tgatcgagcg cagggaaact gcgggcccgg acctcttcag catagccgct gatggcggcc
     1441 tcgatggcct tggacacctc gccgaaggtc ttgacgaact tgggcgggcg cggattgagc
     1501 ccgagcatgt cctccatcac caggatctgt ccgtcgcaga ctgccgatgc gccgatgccg
     1561 atggtgggaa tggggatcgc cttggtgatc gtggccgcca gcggctcggc catggcctcg
     1621 agcacgaccg cgaacgcgcc cgcatcgctg accgctcgcg catcctcctc gatggccgcc
     1681 cactggtccc ggtggcggcc ctgggtgcgg aaaccgccga gcacgttgat ggactgggg
//
LOCUS       DATACA010000035         4633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2575489, whole genome shotgun sequence.
ACCESSION   DATACA010000035 DATACA010000000
VERSION     DATACA010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575489"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1275
                     /locus_tag="VHK28_00725"
     CDS             91..1275
                     /locus_tag="VHK28_00725"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX2140782.1"
                     /translation="MTRPLDVVVAPDSFGGALDSVAVAQAMAAGWSRARPQDKVAMIP
                     MADGGEGTLASLEAALGERAERRMVETSDPLGRPVEAGWLLLDQGRGAFVEMAAASGL
                     AHLEPAERTSESARAATTRGTGELLRAALDAGVEWITLGLGGSATTDGGTGMLSALGA
                     RFLDADGRDLPPGGAALARLRRIDHSNLDPRLAEVRITVASDVTNPLCGPRGAAATYG
                     PQKGADPATVEELDAALARFGDGIEAATGRLVADLPGAGAAGGTTAALLGFTAATVRP
                     GVDVVAEVVGLAEALEAADVVLTGEGRADEQTLSGKAAMGVARLARTRSAPVILLCGA
                     LGAGSAALDASGVFALVQPVGDRPMSLEESMADTERLVTNAAERVARGVDIGLKLADA
                     GR"
     gene            1262..2104
                     /locus_tag="VHK28_00730"
     CDS             1262..2104
                     /locus_tag="VHK28_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2140783.1"
                     /translation="MPAADRSRSRSRRRRGPSPQKLAADRRKRRALATFALERLGDRY
                     EHPRWAGPRLEAVAELVLTILSQNTADTNSFRAFTLLRERYPSWDAVLHAPTDELVEV
                     IRPGGLAPTKAPRIQHVLAEVHEATGGTWDLSFLGTLPLREARDWLTELPGIGRKTAA
                     IILLFNFGRPVLPVDTHVHRVATRLGMLPPRAPLERAHDLLEEVLEPEEMYPFHVELI
                     RHGRDTCRAPRPICGLCPLTDACAYYVQVQRGRAPVAPIPRPRPDEAPRYHDDLIHGW
                     RPPE"
     gene            2141..3088
                     /locus_tag="VHK28_00735"
     CDS             2141..3088
                     /locus_tag="VHK28_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2140784.1"
                     /translation="MDRSRGLSVAVLVVVLALLAAVLAFVLLNQGDPVEPGASTAPSP
                     SPPAETTPSPSLNQALLEERLTVLLIGVDSTEGRRAQGLGANTDTLMLASVGPEQSEV
                     TLIGLPRDTVDIPLPDGSTWDRKVNALYSERGVEALVGAVESLFAVPVDGYAEIDMDD
                     FPALVDSVGGVQVSPPEPLTDPIVDLDLPAGEQVLDGQTALAYVRTRVDTDYGRMARQ
                     QEVLLEVVRGLASPETDVDVRGLLDGLASFETDLPLDELPTLLEIGRRAAEAPVERTI
                     LRPPDFITFEGDAGDGRGYILIPDVEAIRAHAAETIGEE"
     gene            3172..3639
                     /locus_tag="VHK28_00740"
     CDS             3172..3639
                     /locus_tag="VHK28_00740"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2140785.1"
                     /translation="MPDLHGWIVFVHVAGVLIAFLAHGVSAGMAFKLTTTREPAAVRA
                     LIDMSRTALFVFAGALLVVVASGIAAGFTGGHWGRLWIWTSIALFVAVFVAMTPMAAQ
                     RIRAVREILLQEEGAQTADAIATLLDAWRPMPTAVLGVSALLVITWLMMFRPF"
     gene            3723..3797
                     /locus_tag="VHK28_00745"
     tRNA            3723..3797
                     /locus_tag="VHK28_00745"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3755..3757,aa:Thr,seq:cgt)
     gene            4055..>4633
                     /locus_tag="VHK28_00750"
     CDS             4055..>4633
                     /locus_tag="VHK28_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140786.1"
                     /translation="MPRALNPRHASPSSTPAPRGSATTLKLLTALALAAMVFAQALPR
                     PTDASGADVAAGYGSATVGGAGGRLINVSTADAFAAALAASGPRIVQVAAGLYDVGKV
                     RVKDPYLTVVGQPGAVIKGSILVVTDQVILRGLSVLPGDQWDDPAALDAVTINGMSGS
                     VSNVVLDHLTMLWGPDIGGLAILGDVSDVTVQH"
BASE COUNT          620 a         1642 c         1692 g          679 t
ORIGIN      
        1 attccgcacc tggcagcccc cctcgcgtga aatccggctc gatatgtcgg gcacggacgg
       61 tcccagggcc gtaccaccta tcatcggtgc atgacccgac cgctggacgt cgtcgtcgcg
      121 ccggactcct tcggcggagc cctggactcg gtggccgtgg cgcaggccat ggcagccggc
      181 tggagccggg cgcgtccgca ggacaaggtc gcgatgatcc ccatggccga tggcggcgag
      241 gggacgcttg cctcgctgga ggccgccctc ggtgagcgcg ccgagcggcg gatggtggag
      301 accagcgacc cgctgggtcg gccggtcgag gccggatggc tgctgctcga ccagggccgc
      361 ggcgcgttcg tggagatggc cgccgcatcg gggctggcgc acctcgagcc cgcggagcgg
      421 acgtcggaga gcgcgcgcgc cgccacgacg cgcggcaccg gggagctgct gcgggccgcc
      481 ctggacgcgg gcgtggagtg gatcacgctg ggcctgggcg gcagcgccac caccgacggt
      541 ggcacgggca tgctcagcgc gctgggtgcc cgcttcctgg acgcggatgg gcgcgacctg
      601 ccgccgggag gcgcggctct cgcccggctg cgccggatcg accactccaa cctcgaccct
      661 cggctggcgg aggtgcgcat cacggtcgcc tcggacgtca ccaacccgtt gtgcggaccc
      721 cggggagcgg cggccaccta tggcccgcag aagggtgccg atcccgccac ggtcgaggag
      781 ctggatgccg cgctggcccg cttcggcgac ggcatcgagg cggccaccgg ccggctggtg
      841 gccgatttgc cgggggcggg ggccgccggc ggcacgaccg cggcgctgct gggcttcacc
      901 gcggcgaccg tgcggccggg cgtggacgtg gtcgccgagg tcgtcggcct ggccgaggcg
      961 ctggaagcgg ccgacgtggt cctgaccggt gagggccgcg cggacgagca gaccctgtcc
     1021 ggcaaggcgg ccatgggcgt ggcccgactg gcacggacgc ggagcgcgcc agtcatcctg
     1081 ctgtgcggcg ccctgggagc cgggtcggcc gccctcgacg catcgggcgt cttcgcactg
     1141 gtgcagccag tgggagaccg gccgatgagc ctggaggagt ccatggcgga caccgagcgg
     1201 ctggtcacca acgcggccga gcgcgtggcg cgcggcgtgg acatcggtct caagctggca
     1261 gatgccggcc gctgaccgga gccggtccag gtcgcggcgg cgaaggggtc ccagcccgca
     1321 gaagctggcc gccgatcgcc ggaagcggcg cgcgctggcc accttcgcgc tggagcggct
     1381 gggagaccgc tacgagcacc cgcgatgggc cggcccgcgg ctggaggcgg tggccgagct
     1441 ggtgctgacc atcctttccc agaacacggc ggacaccaac tcgttccggg ccttcaccct
     1501 gctgcgcgag cggtacccga gctgggacgc cgtcctgcac gcgccgaccg acgagctggt
     1561 ggaggtcatc cgtcccggcg ggctggcgcc caccaaggcg ccgcgcatcc agcacgtgct
     1621 ggccgaggtg cacgaggcga ctggcgggac gtgggatctg tccttcctgg gcacgctccc
     1681 gttgcgcgag gcgcgtgact ggctgacgga attgccgggc atcgggcgca agacggcggc
     1741 catcatcctg ctcttcaact tcggtcggcc ggtgctgccg gtcgacaccc acgtccaccg
     1801 ggtcgccacc cggctcggca tgctgccgcc ccgcgcaccg ctggagcggg cgcacgacct
     1861 gctggaggag gtgctcgagc cggaagagat gtacccgttc cacgtcgagc tcatccggca
     1921 cggccgtgac acctgccgcg caccacgccc catctgcggc ctctgccccc tgaccgatgc
     1981 ctgcgcgtac tacgtccagg tgcagcgcgg cagggcgccg gtggcgccca tccccaggcc
     2041 acgaccggac gaggcgccgc gttaccacga cgacctgatc cacggctggc gaccccccga
     2101 gtaggcgctg gcacgccagc cgcagtcctc ggaccgcttc atggatcgat cccgaggact
     2161 cagcgttgcg gtactggtcg tggtgctggc gctgctggcc gccgtcctgg ccttcgtgct
     2221 gctgaaccag ggcgacccgg tggagccggg tgcttcgacc gcgccgagcc cctcgcctcc
     2281 cgccgagacg acccccagcc ccagcctcaa ccaggccctg ctcgaggagc ggctgaccgt
     2341 gctgctgatc ggcgtggaca gcaccgaggg gcggcgggcg cagggcctcg gcgcgaacac
     2401 cgacacgctg atgctggcca gcgtggggcc cgagcagtcg gaggtgaccc tgatcggcct
     2461 gccgcgggac acggtcgaca tcccgctgcc ggacggatcg acctgggacc gcaaggtcaa
     2521 cgccctctac tccgagcgcg gcgtggaggc gctggtcggt gccgtggagt cgctcttcgc
     2581 cgtgcccgtg gacggatacg cggagatcga catggacgac ttcccggcgc tggtcgactc
     2641 ggtgggtggc gtgcaggtat cgccgccgga gccgctgacc gatcccatcg tcgacctgga
     2701 cctgcccgcc ggcgagcagg tgctggacgg ccagaccgcg ctggcctacg tccggacccg
     2761 ggtggacacc gattacgggc gcatggctcg ccagcaggag gtgctgctgg aggtggtgcg
     2821 agggctggct tcgccggaga ccgatgtcga cgtgcgcggc ctgctggacg gcctcgcctc
     2881 ctttgagacc gacctgccgc tcgacgagct gccgacgctg ctggagatcg ggcggcgcgc
     2941 ggccgaagcg ccggtggagc gcacgatcct gcggccaccc gacttcatca ccttcgaggg
     3001 cgacgcaggc gacggccgcg gctacatcct catccctgat gtcgaggcca tccgcgccca
     3061 cgccgccgaa acgatcggcg aggaataggc gccggcccgg gggcttgcgg tggctgggta
     3121 gcatcggtcc cgttcagttc tcgttcccga gctgtaacgg aggatcgtcg cgtgcccgac
     3181 ctgcatggct ggatcgtctt cgttcacgtg gccggcgtgc tgatcgcctt cctggcccac
     3241 ggcgtgtcgg ctggcatggc cttcaagctg acgaccaccc gggagccagc cgccgtcagg
     3301 gcactgatcg acatgtcgcg caccgccctg ttcgtcttcg ccggggcgct cctggtggtg
     3361 gtcgccagtg ggatcgccgc cggcttcacc ggcgggcatt ggggacggct gtggatctgg
     3421 acctcgatcg cgctcttcgt ggccgtcttc gtggccatga cgcctatggc agcgcagcgc
     3481 atccgtgccg tgcgcgagat cctgctgcag gaggagggcg cgcagaccgc cgatgcgatc
     3541 gccacgctgc tggacgcctg gcgtccgatg ccgaccgccg tgctcggcgt gagtgccctg
     3601 ctggtcatca cctggctgat gatgttccgg cccttctagg gtcgcggtgg catcggtcct
     3661 ggcgcgctgg tagactcggc gtcgcctcag ccgcgcgacg cggcgcccga gacccgatcc
     3721 gggccgaagt agctcagggg tagagcatcg gtttcgtaaa ccgacggtcg tgggttcgaa
     3781 tcccaccttc ggctccacgt tcagagcacg gttcgccggc ttgcgcctcg ttcaaccggc
     3841 gcacttggcg atctgacgcc aacgcgacca ccatcgcccc agtgacctgg cgggccactt
     3901 ggcatcgcga gcgaacgatc gcgtccgtgt gccctggtac tggcgtgcgc ggtgaccatc
     3961 gggccctcgt tgaggggacg aatgcgtcaa taggcgcgcg gctcatcggc acatagcctc
     4021 ggatgacgac tgaccgccat agggggagac ccgagtgccc cgcgctctca atccacgcca
     4081 cgcctcgcca tcgagcacgc ccgcgccccg cgggtccgcc acgaccttga agctgctgac
     4141 cgcccttgca ctggccgcga tggtcttcgc ccaggcgctg cccagaccca cggatgcctc
     4201 aggtgctgac gtggccgccg gctacggttc cgccaccgtc ggcggagcgg gtggcaggct
     4261 catcaacgtc agcaccgcgg acgcgttcgc ggctgccctg gcggcgagcg gcccgcgcat
     4321 cgtgcaggtg gccgccgggc tctacgacgt cgggaaggtc cgtgtgaagg acccgtacct
     4381 gaccgtggtc ggccagccgg gcgccgtcat caaggggtcc attctggtcg tcaccgacca
     4441 ggtgatcctg cgcggcctca gcgtgctgcc cggcgaccaa tgggacgatc ccgccgccct
     4501 cgacgcggtg accatcaacg gcatgtcggg ttcggtgtcc aacgtggtgc tcgaccacct
     4561 gaccatgctg tggggcccgg acatcggcgg gctggccatc ctgggcgacg tgtcggacgt
     4621 gaccgtccag cac
//
LOCUS       DATACA010000036         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_647499, whole genome shotgun sequence.
ACCESSION   DATACA010000036 DATACA010000000
VERSION     DATACA010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647499"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VHK28_00755"
     CDS             complement(<1..496)
                     /locus_tag="VHK28_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140787.1"
                     /translation="MPELPPRDQLELAAQVLTAILATWLGLTVTVRSPPSPATRAFGL
                     ISLLLVTWSVAIVVQRLSTVPAVDSAFNALEEVGAFLVVAATPHIAIAITAEGRWSLP
                     QRINIWVAYGLAALIGIPSVINPGAEFAITPPHFELPGIPGEVFGWAWILIRMGMFAS
                     ALAWI"
     gene            614..>1575
                     /locus_tag="VHK28_00760"
     CDS             614..>1575
                     /locus_tag="VHK28_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX2140788.1"
                     /translation="MNAPSATIDDVRAVVPSPNQLPIFEELTAAPAARRRPHLRIQPP
                     LLADLTPRQRKAVTHGDGPLLIVAGAGTGKTTVITRRIAWLIAEKRARPSEILALTFT
                     DRAALEMTERVDRLVPYGYTDAVINTFHAFGDRLLREHALEAGLSDRCTVLSRAEQVI
                     FLREHLFELPLDRYRPLGDPTRFLHALVTFISRARDEDVTPAEYRSAAAAIAARAAED
                     PHDQALAEEAARHAELAALYEAYERLMRGSDRIDFGDQVSLALRLLRDHPAVLQAERH
                     RFRYILVDEFQDTNYAQFELVKLLAGDPKANVTVVGDDDQSIYR"
BASE COUNT          254 a          562 c          537 g          222 t
ORIGIN      
        1 cgatccaggc cagcgccgag gcgaacatgc ccatgcggat caggatccag gcccagccga
       61 agacctcgcc ggggatgccg ggcagctcga agtgcggcgg cgtgatggcg aactccgcac
      121 caggattaat caccgatggg atgccgatca gcgcggccag cccgtacgcc acccagatgt
      181 tgatgcgttg cgggagcgac cagcggccct cggcggtgat ggcgatggcg atgtgcgggg
      241 tggccgccac caccaggaag gcgcccacct cctccagggc gttgaatgcg ctgtccaccg
      301 ccgggaccgt cgacagccgc tggacgacga tcgccacgct ccaggtgacg aggagcaggg
      361 agatcaggcc gaaggcgcgc gtggccggcg acggcgggga gcggaccgtg accgtcaggc
      421 ccagccaggt ggccaggatg gcggtcagga cctgggccgc gagctcgagc tgatctcgcg
      481 gcggtagctc gggcaccgtc ccctcctatc ggaccggcag cgcgggcacc ttactctcgc
      541 cttggtgccg gcacaagggc caatcggcca gcgcgacagc cgagctgtct gataagcggt
      601 tgataacggt ccggtgaatg ccccctcggc taccatcgac gacgtgcgtg cggtggtgcc
      661 atccccgaac cagctcccga tcttcgagga gctgaccgcc gcccccgctg cccgtcggcg
      721 tccgcacctc cgcatccagc cgccgctgct ggctgacctc actccccgcc agcgcaaggc
      781 ggtgacccac ggcgacggac cgctgctgat cgtggccggt gccgggacgg gaaagaccac
      841 cgtcatcacc cgtcggatcg cgtggctgat cgccgagaag cgggcgaggc cgtcggagat
      901 tttggccttg accttcaccg accgggcggc gctggagatg accgagcgcg tggatcggct
      961 ggtgccctac ggctacacgg acgccgtcat caacaccttc cacgccttcg gcgaccggct
     1021 gctccgcgag cacgccctgg aggccggcct gtcggaccgc tgcacggtgc tgtcccgcgc
     1081 cgagcaggtc atcttcctgc gcgagcacct cttcgagctt ccgctggacc gataccgccc
     1141 cctcggcgat cccacccgct tcctgcacgc cctggtgacg ttcatcagcc gggccaggga
     1201 tgaggacgtc acgcccgccg agtaccggtc cgcggctgca gccatcgcgg cacgcgccgc
     1261 cgaggacccg cacgaccagg ccctggctga ggaggccgcg cggcatgccg agctggcggc
     1321 gctgtacgag gcctacgagc gcttgatgcg cggcagcgac cgcatcgact tcggtgacca
     1381 ggtgagcctc gccctgcgac tgctgcgcga ccatccggcc gtgctgcagg ctgagcggca
     1441 ccgcttccgc tacatcctgg tggacgagtt ccaggacacc aactacgccc agttcgagct
     1501 ggtgaagctg ctggccggtg acccaaaggc caacgtcacg gtggtggggg acgacgacca
     1561 gagcatctac cgctt
//
LOCUS       DATACA010000037         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_245919, whole genome shotgun sequence.
ACCESSION   DATACA010000037 DATACA010000000
VERSION     DATACA010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245919"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VHK28_00765"
     CDS             complement(<1..357)
                     /locus_tag="VHK28_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2140789.1"
                     /translation="MTTTKVHDPELEISAVLTEELREWLMAELRYPVLAIVDADGAPT
                     QSVMWFDLDPDRPDTILMNTKTNRAKYRWLKREPRVSLLFEDRLTWVGLQGRTELDED
                     SERAMADIKALARRYDS"
     gene            593..1438
                     /locus_tag="VHK28_00770"
     CDS             593..1438
                     /locus_tag="VHK28_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2140790.1"
                     /translation="MRIGFKTSQTIADLSVLLETWDAGEELEVFDSAWIFDHFVALRG
                     EPGPSHEGIALAGALAVRTKRLQFGHLVLGNTYRHPALVANAAVTLDHLAPGRFVLGL
                     GAGWHEGEHAMYGMPLPPIGERITMLESSVRVIKALFDDPDGASLDAPPYRLTDAVLD
                     PPAATPGGPPIWLGTQGVRRGLRIVAQYADGWNATGDPTAFVEKRDALIRHCEAVGRD
                     PTEIEISAQANIRDGHAAALERATTLVDDGVQHMVLIMPAELGPDGLRRLAREVAEPL
                     RERFS"
     gene            1446..>2371
                     /locus_tag="VHK28_00775"
     CDS             1446..>2371
                     /locus_tag="VHK28_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX2140791.1"
                     /translation="MTSAGPRAFLLSIGSELLMGETVDTNAAFLGAELRQLGVTLSEV
                     RQLPDNRDAIAKAFTQAAVLADVVLATGGLGPTHDDLTREALAYALGEELTLDSELER
                     QLRERFGALGRMPESNLKQAMRVPSARILANPIGSAPGWWVERGEGVVVLMPGVPSEM
                     RLMWADEVAPRLRALLDVPPLAVRTVKTFGVGESAVAEALGSLLKSPGEGVEAGIYAR
                     DDGVHLRFSTGADASKLDPAVDRALDVLGADVWGTDDDDLASVTLLALKRAGARTAAS
                     WEADTGGALLAVLAATAPGEGAARYLGGVLEV"
BASE COUNT          355 a          812 c          836 g          368 t
ORIGIN      
        1 ggagtcgtag cgtcgggcaa gggccttgat gtcagccatg gcccgctccg agtcctcgtc
       61 cagctccgtc cggccctgga gcccgaccca ggtaagccta tcctcgaaca ggaggctcac
      121 gcgcggctcg cgcttcagcc atcggtactt agcgcggttg gtcttggtgt tcatcaaaat
      181 ggtgtccggc cggtcggggt cgaggtcgaa ccacatcacc gactgggtcg gcgccccgtc
      241 ggcatccacg atggccagca ccgggtagcg cagctcagcc atcagccact cccgcagctc
      301 ttcggtcagg accgcggaga tttccagttc ggggtcatgc accttcgtcg tcgtcatgta
      361 cgcatcgtag accgatgggc cctcacgcga gccggcgccg ataaatcgcg ccggattgca
      421 cgcgacgggc tggtggcgtg tcgttctgcc gatctgtcgg gcagcagcga gcgtgaaggg
      481 cgaagaacct gtcgcctcgt gcgtgggggt acccgatgta tcggcgatcc gacacgcagt
      541 ggaccctccc ggtgaaggac tgcccgatga atcgggccag tacactcccc cgatgcgcat
      601 cgggttcaag acctcgcaga cgatcgccga cctatcggtc ctgctggaga cgtgggatgc
      661 cggcgaggag ctggaggtct tcgactcggc ctggattttc gaccacttcg tggcgctgag
      721 gggcgagccg ggcccgagcc acgaggggat cgcgctggcc ggcgcgctcg ccgtacgcac
      781 gaagcgactg cagttcgggc accttgtgct gggcaacacg taccgccacc cggcactggt
      841 ggccaacgcc gccgtgacgc tcgaccacct ggcgccgggc cgcttcgtgc tggggctggg
      901 cgccggctgg cacgagggcg agcacgccat gtatggcatg ccgctgccac ccatcggcga
      961 acggatcacc atgctcgaat cgtccgtgcg cgtcatcaag gcgctgttcg acgaccccga
     1021 cggtgcatcc ctcgacgcgc cgccctaccg cctgaccgat gcggtcctgg acccaccagc
     1081 cgccaccccg ggcggaccgc ccatctggct cggcacgcag ggggtgcgac gcggactgcg
     1141 aatcgtggcc cagtacgcgg acgggtggaa tgccactggc gacccgaccg cattcgtgga
     1201 aaaacgagac gcactgatac ggcattgcga ggccgtcggc cgcgatccga ccgagatcga
     1261 gatcagcgcc caggccaaca tccgcgatgg ccacgccgcg gcgctcgagc gggccaccac
     1321 cctcgtggat gacggggtcc agcacatggt gctcatcatg ccggccgagc tcgggcccga
     1381 cggcctgcgg cgactggcgc gggaggtggc cgagcccctg cgcgagcggt tcagctgagc
     1441 atcaggtgac cagcgccggt ccgcgcgcct tcctgctgtc catcggcagc gagctgctga
     1501 tgggcgagac ggtcgatacc aacgccgcct tcctgggcgc tgagctgcgg cagctgggcg
     1561 tcaccctgtc cgaagttcgc cagctgccgg acaaccgcga cgcgatcgcc aaggccttca
     1621 cccaggcggc cgtgctcgcc gacgtggtgc tggccaccgg cggcctggga cccacccacg
     1681 acgacctgac gcgcgaggcc ctggcctacg cgctcggcga ggagctgacg ctggactccg
     1741 agctggagcg tcagttgcgg gagcgattcg gcgcactggg ccgcatgccg gagtccaacc
     1801 tgaagcaggc gatgcgagtc ccatcggccc ggatcctggc caatcccatc ggttccgcgc
     1861 ccggctggtg ggtcgagcgc ggggagggcg tggtggtcct gatgccaggc gtgccgtccg
     1921 agatgcggtt gatgtgggcc gacgaagtgg cgccccgcct gcgcgctctc ctggacgtcc
     1981 cgccccttgc ggtgcgtacg gtgaagacct tcggggtcgg cgagtcagcc gttgccgagg
     2041 cgctcggctc gctcctgaag tcgcccggcg aaggggtcga ggccggcatc tacgcccgcg
     2101 acgacggtgt ccatctacgg ttttcgaccg gcgctgacgc ctcgaagctg gacccggcgg
     2161 tggaccgcgc gctggatgtc ctcggagcgg atgtctgggg aaccgatgac gacgacctgg
     2221 cctccgtcac gctcttggcg ctgaagcgag cgggtgcgcg aactgccgcc tcctgggagg
     2281 cggataccgg tggagcgctg ctcgccgtcc tggccgcgac ggcgccgggc gagggagccg
     2341 cgcgctacct cggcggtgtg ctcgaggtcg a
//
LOCUS       DATACA010000038         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2575628, whole genome shotgun sequence.
ACCESSION   DATACA010000038 DATACA010000000
VERSION     DATACA010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575628"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VHK28_00780"
     CDS             <1..357
                     /locus_tag="VHK28_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140792.1"
                     /translation="TASANDNAIVEGITGASSSLGWVGLAFAQEAEGLKSVEVDGGAG
                     CVGASSQAVADESYPISRPLFIYPSLNAVADNPAVEPFVDYYLSPDGLALAGEVGYVP
                     LTDDQGQAARDAWEAR"
     gene            342..1280
                     /gene="pstC"
                     /locus_tag="VHK28_00785"
     CDS             342..1280
                     /gene="pstC"
                     /locus_tag="VHK28_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX2140793.1"
                     /translation="MGGALIARLGISDLRGDPGRRRREAAIRLGFQAAALLSVVISAA
                     IILTLVGEAWSFVSQVDPVRLIDRGWFPRRDMYGIPTIVAGTLSIAGIAMLVAAPLGL
                     GSAMYLSEYAGPRARSMLKPALEVLAGIPSVVLGYFALQVLSPTVVDPLCIGPTDPYT
                     MAAAGIAVGILVTPLVASVAEDAMHAVPTSLREAAYGLGARRRTASLRVVVPAAVSGI
                     VAGLILGMSRAIGETMVVAIAAGGLGGSLFSLDPCGPGQTMTAAMTALATGSDQVRGA
                     SLAFPSLFFVGLLLFAFTLGLNLVANRVVRRMRERY"
     gene            1280..2233
                     /gene="pstA"
                     /locus_tag="VHK28_00790"
     CDS             1280..2233
                     /gene="pstA"
                     /locus_tag="VHK28_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX2140794.1"
                     /translation="MAALATPSRSASLTREVVRQRLTRRHADLGGRVFEGMLMLLLLA
                     SLAVLGWLLAVVAVNGMPTLLDRGVDFFTATLASRAEQAGVAQGITGSLYLMGFVALV
                     AFPLGIAAAVYLEEYAGDTAASRFINAVVRNLAGVPSIVYGLLGLAIFVIALGDVLGP
                     GRTSGRSLLAGGLTMAILVLPIVIITAAEALRAVPASIREAAFAVGATRWEVVRSHVL
                     PYAAPGILTGTILAMARAFGETAPLIVVGAVVGGFNLAYDNPVEQLFGRYTALPTTIY
                     DWAGNPASFAGNVYAAIIVLLVMLLGVNALAVYLRNRYERR"
     gene            2275..>2595
                     /locus_tag="VHK28_00795"
     CDS             2275..>2595
                     /locus_tag="VHK28_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2140795.1"
                     /translation="MATPAAAVAEERVFELRDVSCFYGSFRAVRHVTLDIPRNRITAI
                     IGPSGCGKSTLLRTLNRMNDLVEGARVDGRILYHGVDVYDPQVDPVEVRRRIGMVFQK
                     PNPFP"
BASE COUNT          352 a          904 c          883 g          456 t
ORIGIN      
        1 accgcctccg ccaacgacaa cgccatcgtg gagggcatca ccggcgcctc aagctcgctc
       61 ggctgggtcg gccttgcctt cgcgcaggag gcggagggcc tgaagagcgt ggaggtggac
      121 ggcggcgcag gctgcgtggg cgcctcgagc caggccgtcg ccgacgagtc gtacccgatc
      181 agccgacccc ttttcatcta tcccagcctg aacgcggtcg ccgacaaccc ggctgtcgag
      241 ccgttcgtcg actactacct ctcgcccgat ggcctggccc tggccggcga ggttggctac
      301 gtgccgctca ccgacgatca gggacaggcg gctcgtgatg cgtgggaggc gcgctgatcg
      361 ccaggctggg catctccgac ctgcggggtg accccggccg gcgccgcaga gaggcggcca
      421 tccggctggg gttccaggct gccgcactgc tctcggtggt catcagcgcg gcgatcatcc
      481 tgaccctggt cggcgaggcg tggagcttcg tcagccaggt cgatcctgtg cggctcatcg
      541 accgtggctg gttcccgcga cgcgacatgt acggcattcc gaccatcgtg gccggcacgc
      601 tcagcatcgc cggcatcgcc atgctggtcg ccgcgccgct gggcctggga tcggcgatgt
      661 acctgtctga gtacgccggc ccacgcgcgc gatcgatgct caagcccgcg ctggaggtgc
      721 tggccggaat cccaagcgtg gtgcttggct acttcgcgct gcaggtgctc agtcccaccg
      781 tcgtcgaccc attgtgcatc ggcccgacgg acccgtacac catggcggcc gccgggatcg
      841 cggtcgggat cctggtgact cccctggtgg cgtcggttgc cgaggacgcc atgcacgccg
      901 tcccgacctc gctgcgtgaa gcggcatatg gccttggcgc acgacgtcgc acggcgagcc
      961 tgcgggtcgt ggtgccagcg gcggtctcgg gtatcgtggc cggcctcatc ctgggaatgt
     1021 cgcgcgccat cggcgagacc atggtggtgg ccatcgcggc gggcgggttg ggcggatcgc
     1081 tcttcagcct cgatccttgt ggaccgggac agaccatgac cgcggccatg acggcgctgg
     1141 ccaccggatc ggaccaggtg cgcggagcca gcctggcctt cccaagcctg ttcttcgttg
     1201 gcctgctgct cttcgccttc accctggggc tgaacctggt ggccaaccgc gtcgtgcgcc
     1261 gcatgcggga gcgctactga tggccgcgct cgccacgccc agccgatccg cgagccttac
     1321 ccgcgaggtt gtgcggcaac gcctgacgcg acggcatgcg gacctcggtg gccgggtctt
     1381 cgaagggatg ctcatgctcc tgctgctggc cagcctggcg gtgctgggat ggctgctcgc
     1441 cgtggtggcc gtcaacggta tgcccaccct gctcgaccgc ggcgtcgact tcttcacggc
     1501 cacccttgcc tcgcgtgctg agcaggccgg cgtggcacag ggcatcaccg gctcgctgta
     1561 cctgatggga ttcgtggcac tcgtcgcgtt tccactcgga atcgccgcgg ccgtgtacct
     1621 ggaggagtac gccggcgaca cggccgcctc ccgcttcatc aacgccgtgg ttcgcaacct
     1681 ggcaggggtg ccctccatcg tgtacgggct gctgggcctg gcgatcttcg tgatcgcgct
     1741 gggggacgtt ctgggtccgg gtcgcacatc ggggcgcagc ctcctggccg gtggcctgac
     1801 catggcgatc ctggtcctgc cgatcgtgat catcaccgcc gccgaggcgc tgcgcgccgt
     1861 tcccgcctcc atccgcgagg ccgccttcgc cgtcggtgcc acccggtggg aggtggtgcg
     1921 cagccacgtc ctgccctacg cggcgcccgg aatcctgacc ggcaccatcc tggccatggc
     1981 tcgggccttt ggcgagaccg cgccactgat cgtggtgggt gcagtcgtgg gaggcttcaa
     2041 cctggcatac gacaacccag tggagcagct gttcggtcgc tacaccgcgc tgccgacgac
     2101 catctacgac tgggctggta accccgcctc attcgccggc aacgtgtacg ccgccatcat
     2161 cgtgctgctg gtgatgctgc tgggcgtcaa tgccctggcg gtctacctgc gcaatcggta
     2221 cgagcgcagg tgacaatacc catggtgatc ggtcagcagc tggaaccagc acccatggcg
     2281 accccggcag ccgcggtggc cgaggaacgc gtcttcgagc tgcgtgacgt ctcctgcttc
     2341 tacggatcgt tccgggcggt gcgccacgtc acgctggaca tcccgcgcaa ccggatcacg
     2401 gccatcatcg ggccgtccgg ctgcggcaag agcaccctcc tgcgtacgct caaccggatg
     2461 aacgacctcg tggaaggtgc gcgcgtcgat ggccgaatcc tgtaccacgg cgtcgacgtc
     2521 tatgacccgc aggtcgatcc cgtcgaggtg cggcgccgga tcgggatggt gttccagaag
     2581 ccgaacccat tcccc
//
LOCUS       DATACA010000039         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2816669, whole genome shotgun sequence.
ACCESSION   DATACA010000039 DATACA010000000
VERSION     DATACA010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816669"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VHK28_00800"
     CDS             complement(<1..779)
                     /locus_tag="VHK28_00800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2140796.1"
                     /translation="MSPRALDLLVLGAVALVVRAVAVAIVPWPPFTDPAYYSLIAQRL
                     AEGHGFSSPVLWSFLEVGSSIPDSPMLPVPSNGHWMPLTSVVAASSMALFGHAYIAGT
                     VPLIVLSALLVPLTYLVAWELWAARSTALVAAILALFAGPLLIMYPTTDNFAVFGATG
                     AASLYCSTRAVRAERLGPWLVAAGAFAGLATLARIDGVFLTLGVAAAWFVRRGWTPWR
                     SPVAGGPSLVSGVASAGAFLVVLAPWLARNWSVYGTPLPSTG"
     gene            870..1739
                     /locus_tag="VHK28_00805"
     CDS             870..1739
                     /locus_tag="VHK28_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140797.1"
                     /translation="MNARWVLLVALYLGLGLLGAWYLVDTTSLGADLATYQRAADDLW
                     SSGDPYATAASVPEDYRYRYPPLLAMVIPVLGWPPLWFALLAIATLVPIYVGYRQAGP
                     AGLLPAALLIGAWGQQLLNGNAQAMVVALLAVVPLAGAGGAIGLAVATMLKLHPAFAV
                     VWYVGRREWRLLAWYVGAMAVLTLIQLPWLPAMIRFYLSDPVATETIPGMSLRALGFL
                     PWVAGTVITAAVAVWFARTRYGWLLATLLQLVALPRVLLVNLALLLAAPLPRREARAP
                     ATPPRPVPAARTG"
     gene            complement(1642..>2273)
                     /locus_tag="VHK28_00810"
     CDS             complement(1642..>2273)
                     /locus_tag="VHK28_00810"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX2140798.1"
                     /translation="GNVSTITLLVLLAVARWQDARGGVAIGLLTLLMPKPHLFPVLAY
                     LLVRRPRDFAAAAATMGVGVVVGLVAFGVEPWLAFLGSLRDPLARTFTANIGFSSLFG
                     PAGVIVGLVAGLGLLGVGIVVGGSRGYGLSIIGGILMGPYTFVHYLAGTLVAAEPVLR
                     SRPRWLVPFPWLLVIFPLIPFWLLGLAWVVWRAPEPPDEAAARPVAAPG"
BASE COUNT          383 a          808 c          789 g          293 t
ORIGIN      
        1 ccagtggatg gcagcggggt gccgtagacc gaccagttcc gtgccagcca cggcgccagg
       61 accacaagga aggcaccggc cgacgccacg cccgacacga gcgatgggcc acccgccacc
      121 ggcgaccgcc atggcgtcca gccccgacga acgaaccacg ccgcggcgac gccaagcgtg
      181 aggaagaccc cgtcgatgcg cgccaaggtg gccaagcccg cgaatgcccc ggcggcgacg
      241 agccatggac caaggcgctc cgcgcgcacg gcgcgagtgg agcagtacag ggatgccgcg
      301 ccggtcgccc cgaagaccgc gaagttgtcg gtggtcgggt acatgatcag gagcggcccc
      361 gcgaagaggg cgaggatggc ggcgaccagc gcggtcgatc gtgcggccca tagctcccac
      421 gccaccagat aggtcagggg caccaagagg gccgagagga cgatgagcgg gacagtgccc
      481 gcgatgtagg catgcccgaa cagcgccatg gaagaggccg ccacgacgga ggtcaacggc
      541 atccagtgac cgttgctggg caccggcagc atcggtgaat cggggatcga gctgccaacc
      601 tccaggaagc tccacagcac gggtgagctg aagccatggc cctccgccag ccgctgcgcg
      661 atgagggagt agtacgccgg atccgtgaat ggcggccagg gaacgatggc cactgcgacc
      721 gctcggacca ccagcgccac ggcgcctagg acgagcaggt cgagcgcacg gggtgacatc
      781 ggcgcgcagt gtagcggcca cccgcggcgg cgccccccgc ccagaggtac cagccgcgtc
      841 ccggtgggcg actgctaccg ttgccgccca tgaacgcgcg ctgggtgctc cttgtcgccc
      901 tgtatctcgg tctcggcctg ctcggagcgt ggtacctcgt cgacacgaca tcgctcggcg
      961 ccgatctggc gacctaccag cgtgcagccg acgacctgtg gtcaagcggc gatccatacg
     1021 ccaccgcggc aagcgtcccc gaggactacc gctaccgcta cccacctctg ctggcgatgg
     1081 tcatccccgt cctcggctgg ccaccgctgt ggtttgccct cctcgcgatc gcgaccctgg
     1141 tgccgatcta tgtcggctac cggcaggccg gtccggcggg gctcctgccc gcggcgcttc
     1201 tcatcggcgc gtgggggcag cagctgctca acggcaatgc ccaggccatg gtcgtggcgc
     1261 tgctggcggt tgtgccgctg gcgggcgccg gaggcgccat cggcctggcc gtggcaacca
     1321 tgctgaagct gcacccggcg ttcgccgtgg tctggtacgt cggacgccgc gagtggcggc
     1381 tcctcgcctg gtacgtgggc gccatggcgg tgctcaccct catccagctg ccgtggctgc
     1441 cggcaatgat ccggttctac ctctccgacc ccgtggcgac tgagacgatc cccggcatgt
     1501 cactccgcgc gctcgggttc ctgccgtggg tggccgggac cgtgatcacc gccgccgtcg
     1561 ccgtctggtt cgcgcgtacc cgttatgggt ggctcctggc cacgctgctc cagctggtcg
     1621 cgctgccccg ggtgctgctc gtcaacctgg cgctgctact ggccgcgccg ctgcctcgtc
     1681 gggaggctcg ggcgcccgcc acaccaccca ggccagtccc agcagccaga acgggatgag
     1741 cgggaagatg acgagcaacc agggaaacgg cacgagccag cgcggccggc tgcgcagcac
     1801 tggctccgcc gcgaccagcg tgccagcgag gtagtgcacg aaggtgtatg ggcccatgag
     1861 gatgcccccg atgatgctca gcccgtagcc tcgcgagccg ccgaccacga tcccgactcc
     1921 caggaggccg agaccggcga ccaagccgac gatgacaccg gccggcccga acagcgatga
     1981 gaagccgatg ttcgcggtga acgtgcgcgc aagcgggtcg cgcagcgagc ccaggaatgc
     2041 cagccacggc tcgaccccga aggcgacgag cccgaccacc acgccgacac ccatggtggc
     2101 tgcggcagcc gcgaagtcgc gtggacgccg aaccagcaga tatgccaaca cggggaacag
     2161 gtgcggtttc ggcatgagca gcgtcagcag gccgatggct acgccgccgc gcgcatcctg
     2221 ccagcgcgcc actgccaaca ggacgagcag ggtgatcgtg ctgacgttgc cga
//
LOCUS       DATACA010000040         4133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2736451, whole genome shotgun sequence.
ACCESSION   DATACA010000040 DATACA010000000
VERSION     DATACA010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736451"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VHK28_00815"
     CDS             <1..978
                     /locus_tag="VHK28_00815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2140799.1"
                     /translation="SIGAIWLLAYVAGVLASGERRMRATVLQLSRTDTLTSLFNRSQL
                     FPTLEQEVQRTRRSERGFCLLMIDLDGLKAVNDSFGHHRGDEVLRSLGNVIRRSIRSV
                     DTAYRYGGDEFLVLLPETDFAGAFVVAEKIRAGAEAVGLAAGGGDLLTSVSIGLVSCP
                     EDGTSAEELMIAADRAMYQAKSLGKNQVSGNPRPPRLPYRPEPPEGEPEPPVSEVPPI
                     QMPHVGGAPVPGLGRAEQAIEEPPIPAAFSVEPEARVSVPVAEREREPESAATATVGG
                     GENGDEEEADPSEVRRHIAAARLHMDPDHQIRRAMDAFLSPGRPNGEPH"
     gene            complement(986..2131)
                     /locus_tag="VHK28_00820"
     CDS             complement(986..2131)
                     /locus_tag="VHK28_00820"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEX2140800.1"
                     /translation="MRHLRSRRVQASLAAGLVLVLAVTAIWLANAEGTASVGATPTPL
                     PSSAEPSPSHTVAPSPTATATASAEPVARCPLNGLPLDDAAVLDSTALAVQIENHPAA
                     RPARNLTRADLVVEATVEGDTTRFTAIVLCRPTEGLTGPVRSARYYNIDLWQDLGVLT
                     VGFGASNGALDRFASAGMPYVNGITGSWPWFRRFGTHAAPHNLYGDMGALRAALDDNA
                     ALARLASDVRPLRPPFTFDTEVVLPDGRSVTSLEIRTNSYWRIGWRWDAGLGAWRRMD
                     AGADISDAATDEPVTAGTVLVQRVTQETVYGDPDPGGNPRRLQHLVGSGSGTVYVRGS
                     AVDVLWSRPSADAGTTWTYADGGESVVLPPGVVWWEIVPVEASIAED"
     gene            2197..3603
                     /locus_tag="VHK28_00825"
     CDS             2197..3603
                     /locus_tag="VHK28_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2140801.1"
                     /translation="MIDARSQAAPRADAGPAVARPAIMRTPDAGAALAALLSFIFPGL
                     GQAYNGQARLAWLLATPVLLLAVGAALALTVARANLLSQALHISFLVGLIVLDLALLG
                     WRLVAITQAHAGRERVSPRRWTAWTTALLLLATFAMHALPAWYAVKAIDTLSTVSQGG
                     GVTRGGGGISRIPELDDLPPPSDQPEVERGERVNVLLVGVDSAPGRTHRLTDTMLVVS
                     LDPNSGRSAMVSVPRDLYGVPLGDGRIFNAKLNSMMSFAEARPAEFPLGGVGTLKAAL
                     GELLGVEIHYFAAIDLLGFSSAIDAVGGVDITVERAISDPTYVDESGNRTGFYLDPGR
                     YHFDGHDALAYVRSRMGVGDNDFTRADRQQQLLAAVREKLTAGNLVLALPSLLDAVKS
                     SLSTDIPSARLPDLAQAVQEADMSQLRRIVLQPPVHMTADPGSAAGYILIPNLEAIRQ
                     VGQEMLSSPPPEASGGPS"
     gene            3639..>4133
                     /locus_tag="VHK28_00830"
     CDS             3639..>4133
                     /locus_tag="VHK28_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2140802.1"
                     /translation="MPGFATRAIRAASRVPAAPQAPVNVPIYQTSTFEVGSAEELAEL
                     LEFARPGHSYSRYSNPTHAALEEALAELEGAEACHVTGSGMAAIHGVAISVLRTGDEM
                     LIPRAVYGGVIGLAHAILEPAGISVRAVDTTDLDAVAEAIGPRTRLVWIETISNPTTA
                     LADVA"
BASE COUNT          589 a         1473 c         1470 g          601 t
ORIGIN      
        1 tccattggtg ccatctggct gctggcctac gttgccggcg tcttagcctc gggtgagcgc
       61 cggatgcgcg cgaccgtcct tcagctgtca cggaccgata cgctgaccag cctcttcaac
      121 cgcagccagc tgttccccac cctggagcag gaggtgcagc gcacccggcg cagtgagcgc
      181 ggcttctgcc tgctgatgat cgacctggac ggcctcaaag cggtgaacga ctcgttcggc
      241 caccatcgag gagacgaggt gctccgatcg ctgggcaacg tcatccgtcg ctcgatccgc
      301 agcgtggaca ccgcctaccg ctacggtggt gacgagttcc tggtgctcct gccggagacc
      361 gatttcgccg gcgcattcgt ggtcgccgag aagatccggg ccggcgccga ggcggtcggc
      421 ctggcggcgg gcgggggaga cctgctgact agcgtcagca tcggtctggt gtcctgtccg
      481 gaggacggaa ccagcgcgga ggagctgatg atcgcggcgg accgggccat gtaccaggca
      541 aagtcgctcg gcaagaacca ggtgagcggc aacccgcgtc cgccgcgcct gccgtaccgc
      601 cccgagccgc cggaagggga gccggagccg ccggtctcgg aggtgccccc catccagatg
      661 ccgcacgttg gaggggcacc cgtgcccggg ctgggccggg cggagcaggc gatcgaggag
      721 ccgccgattc cggcggcctt ctccgtggaa cccgaggcac gcgtcagcgt tccggtggcg
      781 gaacgggagc gcgagccgga gtcggccgcc acggccaccg tcggcggcgg tgagaacggc
      841 gacgaggagg aggccgatcc gagcgaggtg cgccggcaca tcgcagcggc gcgattgcac
      901 atggaccccg accaccagat ccggcgtgcc atggacgcct ttctgtcacc cggtcggccg
      961 aacggcgagc cgcactgagt gcgcctcagt cctcggcgat ggacgcctcc accgggacga
     1021 tctcccacca caccacgccc ggtggcagca ccacggactc gccaccatct gcataggtcc
     1081 aggtggtccc ggcatccgcg ctgggacgtg accacagcac gtcgacggcg ctgcccctca
     1141 cgtagaccgt gccgctgccg ctgccgacca ggtgctgcag ccggcgcggg ttgccgcccg
     1201 ggtccgggtc gccgtacacc gtctcctgcg tcacgcgctg gaccagcacg gtgcccgccg
     1261 tgaccggctc gtcggtggca gcatccgaga tgtcggcgcc ggcatccatc cgccgccacg
     1321 cccccaggcc ggcatcccac cgccagccga tccgccagta ggagttggtg cggatctcca
     1381 gggacgtgac cgatcgcccg tccggcagca ccacctcggt gtcgaaggtg aacgggggcc
     1441 gcagcgggcg gacgtcggat gccaggcgcg ccagtgccgc gttgtcgtcc agcgccgcgc
     1501 gcagcgcccc catgtcgccg tacaggttgt gcggggcagc gtgggtcccg aagcgccgga
     1561 accagggcca cgagcccgtg atcccattga catacggcat gccggccgag gcgaagcggt
     1621 cgagagcgcc gttgctggcg ccgaagccca cggtcagcac gcccaggtcc tgccacaggt
     1681 cgatgttgta gtaccgcgcg ctgcgcaccg ggccggtcag gccctcggtc ggcctgcaca
     1741 ggacgatggc ggtgaagcgg gtggtgtcgc cctccacggt cgcctccacc accaggtcgg
     1801 cccgggtcag gttgcgcgcg ggtcgggcgg cgggatggtt ctcgatctgg accgcgagcg
     1861 cggtcgagtc caggaccgcg gcgtcgtcca gtggcaggcc gttgagcggg cagcgcgcga
     1921 ccggctcggc gctcgccgtg gccgtggcgg tggggcttgg ggccacggta tggctgggcg
     1981 agggttcggc gctcgagggc agtggcgtgg gcgtggcgcc gaccgaggcc gtgccctcgg
     2041 cgttcgccag ccagatggcg gtgaccgcca gcaccagcac gaggccagcc gccagcgagg
     2101 cctggacgcg gcgggagcga aggtgtcgca tcgcaacgaa tgctagtagg cgagcgtcaa
     2161 gcctccatgg caccattggg ccatggaggt cccgcattga tcgacgcccg gtctcaggct
     2221 gccccgcgtg cggatgccgg accggcggtc gcccgaccgg cgatcatgcg cacgccggat
     2281 gcgggcgctg ctctggccgc gctgctgtcg ttcatcttcc ccgggcttgg ccaggcatac
     2341 aacgggcagg cgaggctggc ctggctgctg gccacgccgg tcctgctgct ggccgtgggg
     2401 gcggcgctgg cgctgacggt tgcgcgcgca aacctgttga gccaggcgct tcacatcagc
     2461 ttcctggtgg gactcatcgt cctcgacctg gcgctgctcg gctggcgcct ggtggccatc
     2521 acccaggccc acgccgggcg agagcgggtc tccccacggc gctggacggc gtggaccacg
     2581 gccctcctgc tgctggccac cttcgccatg cacgccctgc ccgcgtggta cgcggtcaag
     2641 gcgattgaca cgctgagcac ggtctcgcaa ggcggcggcg taacccgcgg tggcggcggc
     2701 atctcgcgca ttccggagct ggacgacctg ccgccaccat ccgaccagcc ggaggtggag
     2761 cgcggcgagc gtgtcaacgt gctgctggtg ggcgtcgact ccgccccggg ccgcacccac
     2821 cggctgaccg acacgatgct ggttgtcagc ctggatccga acagcgggcg atcggccatg
     2881 gtctcggtcc cgcgcgacct gtacggcgtc ccgctgggcg acggccgtat cttcaacgcc
     2941 aagctcaact ccatgatgtc cttcgccgag gcacgtccgg ccgagttccc gctgggcggc
     3001 gtcggaaccc tcaaggcggc gctgggcgag ctgctgggcg tcgagattca ctacttcgcg
     3061 gccatcgacc ttctcggctt cagcagcgca atcgacgcgg tgggtggggt ggacatcacc
     3121 gtcgaacgag ccatcagcga cccgacctac gtagacgaga gcgggaaccg cacgggcttc
     3181 tacctggatc ccgggcggta ccacttcgat ggtcatgacg cgcttgccta cgtgcggtcc
     3241 cgcatggggg tgggtgacaa cgacttcacc cgcgccgacc gtcagcagca gctgctggcc
     3301 gccgttcgcg agaagctgac ggccgggaac ctggtgctcg ccctcccgag cctgctggac
     3361 gcggtcaagt cctccctgtc gaccgacatc ccctccgccc ggctcccgga cctggcgcag
     3421 gcggtccagg aggccgatat gtcccagctg cgccgcatcg tcctccagcc gccggtgcac
     3481 atgacggccg atccgggttc cgcggcgggg tacatcctga ttcccaacct ggaggcgatc
     3541 cgccaggtgg gccaggagat gctctcctcg ccgccaccgg aggcgagcgg cggcccctcc
     3601 tagggcaccg cgggcctgct gctagactcg gcccggtcat gccaggcttc gccacccgcg
     3661 cgatccgagc cgccagccgg gtgccggcgg cgccgcaggc gccggtcaac gtgccgatct
     3721 accagaccag caccttcgag gtcggatcgg ccgaggagct cgccgagctg ctggagttcg
     3781 cccgccccgg ccattcctac agccgctact cgaacccgac gcatgcggcg ctggaggagg
     3841 cattggccga gctggagggc gcggaggcct gccacgtcac cggttccggc atggccgcca
     3901 tccacggcgt ggcgatctcc gtcctgcgta ccggtgacga gatgctcatc ccgcgcgccg
     3961 tgtacggtgg ggtcatcggc ctggcgcatg ccatcctgga gcccgccggc atctcggtgc
     4021 gagccgttga caccaccgat ctggacgcgg tggccgaggc catcggcccg cggacccggc
     4081 tggtgtggat cgagaccatc agcaacccca ccaccgcgct ggccgacgtg gcg
//
LOCUS       DATACA010000041         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1933781, whole genome shotgun sequence.
ACCESSION   DATACA010000041 DATACA010000000
VERSION     DATACA010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933781"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..981
                     /locus_tag="VHK28_00835"
     CDS             274..981
                     /locus_tag="VHK28_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2140803.1"
                     /translation="MGARILVVEDEPRLRELLRLYLEREGYRVTDAGDGDSALAAYET
                     DGADLVVLDLMLPGLQGEAVLEALREAGDVPVLITSAKRSDAERIAGLRLGADDYLGK
                     PFNPHELTARVAAILRRTRPTAAGEDSILSLAGGRLVLDARSRRYTLADATTGRLTPG
                     ECALLVALARRPGAVFTRDQLLEAVARRPDEVYERVVDVHVANLRRKLGDDAGQPWLI
                     ETVPSTGYRLVASLDSP"
     gene            978..2324
                     /locus_tag="VHK28_00840"
     CDS             978..2324
                     /locus_tag="VHK28_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2140804.1"
                     /translation="MTATSAARRSRGGTPLSIRLAMLLSLAVVAVLLVTGLAVNRFVS
                     RSLEEQLTTSQRSQIELLASQLQGADLSQPGSRRAVDMAFRRIARPIDGRIELIAADG
                     TLLAARGNVRGGIGTERIEEPVPGDAGWTLIVEFPSGQRPFLSVFNATLLVAGIAAVV
                     ALMLAATLLSDRLTRPLRAVAAAANRLGSGDLSARAEGGTDRESTELAEAFNAMAERL
                     ERSEGLRRRAASDIAHDLATPATVLESQLQAMVDGVVPMDRTELDRARASAAALSGVV
                     GQLRDLVDVEAAPLHRRASVISLDDLLAEAADALEPLYREASVQLELAPAPEGLHVEV
                     DSSQMGRVLRNVLTNAAQHSPPGARVRVEAGAEGNAVILRVSDQGRGIATEDVPHVFE
                     RFYRADPARSGGGDSRGSGIGLTVARELVRANGGEVWVERTDPHGTTFAISLPLAG"
     gene            complement(2391..>2733)
                     /locus_tag="VHK28_00845"
     CDS             complement(2391..>2733)
                     /locus_tag="VHK28_00845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2140805.1"
                     /translation="GNVMLRDVDRENGSAEASIVITDKRLQGQGLGTDAMNCLLDFGF
                     GELRLERISLRVFDYNRRARRSYEKSGFQVDAVLHRSRFYRGEHHDVEVMSILRGEWL
                     AVDRRRAWDPA"
BASE COUNT          399 a          884 c         1040 g          410 t
ORIGIN      
        1 cggggccgtg ggcatcgggc gcgatcgagg attcatcggt ggcaggggcg gccatgacgc
       61 tgccgatcag cgtgccgcca acgatggcgg tggcgaccag caggccggcg cctcccagga
      121 gcttcgcgaa ggacatggtt cgtgcacctc tcgttgatgc ggttgctggg cggttgtgag
      181 gggagcatcg gtcgggggac gtgaagaacg ggtcaagatg ggcgccatcg gtcttgacat
      241 ctcctgaatg cacggcgcac taggattcgg cgcatgggtg cccggatcct ggtggtcgag
      301 gacgagccgc gcctgcgcga gctgctgcgg ctgtacctgg agcgcgaggg ttaccgggtg
      361 accgatgccg gtgacggcga ctccgccctg gccgcgtacg agaccgatgg tgccgacctg
      421 gtcgtcctgg acctgatgct ccccggcctg cagggcgagg cggtgctgga ggcgctccgt
      481 gaggctgggg acgtgccggt cctcatcacc tcggccaagc ggtccgacgc ggagcgaatc
      541 gccggcctgc gcctgggcgc cgatgactac ctgggcaagc cgttcaaccc tcacgagctg
      601 acggcgcgag tggcggccat cctgcgccga acgcgtccaa ccgcggccgg ggaggacagc
      661 atcctgtcgc tggccggtgg ccgcctggtg ctcgatgcga gatcacggcg ctacacgctg
      721 gccgatgcca cgaccggccg cttgacgccc ggcgagtgcg cgctgctggt ggcgctggcg
      781 cgccggccgg gagcggtctt tacccgcgac cagctcctgg aggcggtggc tcggcggccc
      841 gacgaggtgt acgagcgcgt ggtggacgtc cacgtcgcga acctgcggcg caagctggga
      901 gacgatgccg ggcagccgtg gctgatcgag acggtgccat caaccggcta tcggctggtg
      961 gccagcctgg actcgccgtg accgcgacat ccgcggcacg gaggtcgcgc ggtggcaccc
     1021 cactctccat ccggctggcg atgctgctgt cgctggcggt ggtggccgtg ctgctggtga
     1081 ccgggctggc ggtcaaccgg ttcgtgagcc gctcgctgga ggaacagcta accacctcac
     1141 agcgcagcca gatcgagctg ctcgccagcc agctgcaggg tgccgatctg tcgcagccag
     1201 ggtcacgacg ggcggtggac atggctttcc gtcggatcgc gaggccgatc gacggccgta
     1261 tcgagctgat cgccgccgac gggacgctgc tggccgcccg gggaaacgtg cgtggaggga
     1321 tcgggacgga gcgcatcgag gagccggtgc cgggcgacgc cggctggacg ttgatcgtcg
     1381 agttcccgtc cgggcagcgg ccgtttctgt ccgtcttcaa tgccacgctg ctggtggccg
     1441 gcatcgccgc cgtggtggcg ctgatgctgg cggccacgct gctctcggat cggctcaccc
     1501 gacccttgcg cgccgttgcc gcggccgcca accggttggg gagcggcgac ctgtcggccc
     1561 gcgcggaggg tgggaccgat cgggagtcga cggagctggc ggaggccttc aacgccatgg
     1621 ccgagcggct cgagcgctcg gagggcctgc gtcgacgcgc ggcgagtgac atcgcgcacg
     1681 acctggcgac gcccgccaca gtgctcgagt cacagctgca ggccatggtt gacggcgtgg
     1741 tgccgatgga tcggacggag ctggatcgcg cccgagcctc tgccgccgca ctcagcggcg
     1801 tcgtcgggca gctgcgggac ctggtggacg tggaggccgc accgctgcac cgccgagcgt
     1861 ccgtgatctc cctcgacgac ctgcttgcag aggcagctga cgcgctggag ccgctctacc
     1921 gggaggcctc cgtccagctc gagctggcgc ctgcgccgga agggctgcac gtcgaggttg
     1981 attcgagcca gatgggacgc gtgctgcgca acgtgctgac caacgcggcc cagcactcgc
     2041 cgccgggtgc gcgggtgagg gtggaggccg gggcggaagg caacgccgtg atcctgcgtg
     2101 tcagcgacca gggacgcggt atcgccacgg aggacgtgcc gcacgtcttc gagcgcttct
     2161 atcgcgccga cccagcccgt tcaggcggag gagatagccg tgggagtggc atcggtctca
     2221 cggtggcccg cgagctggtg cgcgccaacg gcggggaagt gtgggtggag cggaccgacc
     2281 cgcatggcac gacgttcgcg atcagcctgc cgctcgccgg ctaggcgaaa aaggcggcag
     2341 gttgccgtcc ggcgcaaacg aggcgcacgc agcgcatggc gccggaggcg ctaggcggga
     2401 tcccaggcgc ggcgccggtc gactgccagc cattccccgc gcaggatgga catgacctcg
     2461 acgtcgtggt gctcaccacg gtagaagcgg gagcgatgca ggacggcatc gacctggaag
     2521 cccgacttct cataggagcg gcgggcccgt cggttgtagt cgaagacgcg caaggagatg
     2581 cgctcaagac ggagctcgcc aaagccgaag tcgagcaggc agttcatcgc gtcggtcccc
     2641 aggccctgtc cctggagccg cttgtcagtg atgacaatgg atgcctctgc gctgccgttc
     2701 tcgcggtcga cgtcgcgcag catgacgttg ccc
//
LOCUS       DATACA010000042         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2416036, whole genome shotgun sequence.
ACCESSION   DATACA010000042 DATACA010000000
VERSION     DATACA010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416036"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VHK28_00850"
     CDS             <1..485
                     /locus_tag="VHK28_00850"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HEX2140806.1"
                     /translation="FMEQQRTSLLRSVANGLGAGLGATMIMSLVMVAGERLGLLGQHP
                     PSKLSDRLLDRVTPFRSREETDAVGTMIHLGIGAGLGAAFALLRRLVPLFRIPGAGVA
                     YALGVWATSYAGLLPALKIMPMPGNDRPGRPPVMIAAHVVFGLALGLLTRRSARRLAR
                     "
     gene            482..1024
                     /locus_tag="VHK28_00855"
     CDS             482..1024
                     /locus_tag="VHK28_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX2140807.1"
                     /translation="MSGDPNTPPGWSYNPSDWANRVPIIVAALVGAAIAAYLAAYQLR
                     LIESAWDPFFGAGTERILDTPISRVLPIPDALLGALAYLVDAVAGAIGGQDRWRRMPW
                     IVILFGIAVGPLGAVSIGLVIIQPVLYNDFCTLCLATAFISVVMIGPAMDEVLATLQH
                     VRREMRDGRGLWPAVLGADR"
     gene            1021..1359
                     /locus_tag="VHK28_00860"
     CDS             1021..1359
                     /locus_tag="VHK28_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140808.1"
                     /translation="MIWQVLAAAVGIWLMAAPAVLGYAGTPADDVDRILGPLAASIGL
                     IAVTEATRGFRRANYLVALALGVLPWVLGYPTLAIVNSLASALALAVLASLNGRVEGR
                     FGGGWRALVE"
     gene            complement(1482..1910)
                     /locus_tag="VHK28_00865"
     CDS             complement(1482..1910)
                     /locus_tag="VHK28_00865"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX2140809.1"
                     /translation="MTRAARYAYVILAWAFLVGVVVQVFFIGLGLFADNVGIELHVTL
                     GWILHLAPIVVLLAAALSRAGRSHWLWALALAAVIFVVPILATLRDSSPIAAAFHPVA
                     AILGFWLAIVVARNSLEAFAEPRPVEVGDEPRAIEESPST"
     gene            complement(1907..2512)
                     /locus_tag="VHK28_00870"
     CDS             complement(1907..2512)
                     /locus_tag="VHK28_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140810.1"
                     /translation="MTAPDGWVCKVCWKWNRAQDERCYKCKTVRGADEAQVEEQRKEL
                     EARKAKTQEDQPESVPDALVAVPVIIFRGYSKAWLRGGIGTFGFLVLLGLGGVTDLGW
                     VALTAIIGAGLVICGLMAGEVAEGMRNREVWAYLAGIALSVVGGVGSVFAFNTFVPGL
                     VSESAVRWGSLIVFGGAAIAAAAGLVMLWRAPREETGGSPS"
     gene            complement(2571..4088)
                     /locus_tag="VHK28_00875"
     CDS             complement(2571..4088)
                     /locus_tag="VHK28_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020522379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2140811.1"
                     /translation="MDVLGDLANGFATALSVENLLFAATGVLLGTLVGVLPGIGPALT
                     VALLLPITYNFEPTGAFIMFAGIYYGGMYGGSTTSILLNTPGESASVVTTIEGFQMAK
                     QGRGGAALATAAVGSFVAGTISTLAITFLAPVVAAFARGFQPADYVGLMVVAFVSVTA
                     LIGRSLLRGMISLFVGLFIGLIGIDNLTGQERFTFGVPQLLNGLDIVVVAVGLFAVGE
                     ALYVASRMRHDRQEIVPVRGRLLMSREELGRSWKPWLRGSAIGFPIGALPAGGAEIPT
                     FLSYNIEKRLAPRSPWGKGAIEGVAGPEAANNAAFSGVLVPLLTLGIPTSATAAILLA
                     AFQIYNLQPGPLLFQNSPDLVWALIASLYLGNFMLLLLNLPLIRLWVKILAIPRALLY
                     ASILVFATLGVYSLANSLVDVLVMYAIGVLGFFMRRYDFPVGPMILGVILAPMLEAQF
                     RRALQISQGDLSVFFTRPISAILLLTAAAALLLPYLPRMLGRARGRLRFGDGAED"
     gene            complement(4101..4625)
                     /locus_tag="VHK28_00880"
     CDS             complement(4101..4625)
                     /locus_tag="VHK28_00880"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2140812.1"
                     /translation="MADPGRGLLGPRFVAAVLVLGAIGLVVSALGIARGGGYSVIGPA
                     TIPLVVATGLLVLGALFAVRTTVRPDEDLARQAAEEESATHWPTVGLVGAVLVAYALA
                     LDGVRLGPLDIPGLGYPLATGLFLPAVARTLGSRALLRDVIAGVGLALAVYFGFTQFL
                     GVRLPPGVLDFVLP"
BASE COUNT          780 a         1651 c         1565 g          648 t
ORIGIN      
        1 cgttcatgga acagcaacgc acctcgctgc tgcgcagcgt cgccaacggc ctgggcgccg
       61 gactgggcgc cacgatgatc atgagcctgg tgatggtcgc cggggaacgg ctgggcctcc
      121 tgggccagca tccgccgtcc aagctcagcg accgcctgct cgaccgggtc actcccttcc
      181 gctcacggga ggagaccgat gccgtgggca ccatgatcca cctcggcatc ggtgccgggc
      241 tgggcgcagc cttcgcgctg ctgcgccgac tggtgcccct gttccgcatc cccggcgccg
      301 gggtggccta cgcgctcggg gtgtgggcca cgtcctacgc cgggctgctg cccgcgctca
      361 agatcatgcc catgccgggc aacgatcgac ccggacggcc cccggtcatg atcgccgcgc
      421 acgtcgtctt tgggctggcg ctcggcctcc tcacccgtcg ttcggcgcga cgtctggcgc
      481 ggtgagcggc gacccgaaca cgccacccgg ctggagctac aacccgtcgg actgggcgaa
      541 ccgggtgccg atcatcgtgg ccgccctggt gggcgccgcc atcgccgcct atctggccgc
      601 ctaccagctg cggctgatcg agagcgcctg ggacccgttc ttcggtgccg gcacggagcg
      661 catcctggac acgcccatct cgcgcgtgct cccaatcccg gatgcgctgc tcggcgcgct
      721 cgcctacctg gtcgacgccg tcgcaggggc cattggtgga caggaccgat ggcgccgcat
      781 gccgtggatc gtcatcctct tcggcatcgc ggtcgggccg ctgggcgcgg tcagcattgg
      841 tctggtcatc atccagccgg tcctctacaa cgacttctgc acgctgtgcc tggcgaccgc
      901 cttcatctcg gtggtgatga tcggcccggc catggacgag gtgctggcca ccctccagca
      961 cgtacgacgg gagatgcgcg acggccgcgg cctgtggcct gccgtcctgg gggcagatcg
     1021 atgatctggc aggtgctggc cgccgcggtc gggatctggc tgatggccgc cccggctgtg
     1081 ctgggctacg ccgggacgcc ggcggatgac gtggatcgca tcctcggtcc gctggcggcc
     1141 agcatcgggt tgatcgcggt caccgaggcc acgcgcgggt tccgacgggc gaactacctg
     1201 gtcgcgctgg cgctgggggt gctgccctgg gtgctcggct accccacgct tgccatcgtc
     1261 aacagcctgg cgagcgccct ggcgctggcc gtcctggcct cgctgaacgg gcgcgtggag
     1321 ggccgattcg gcggaggatg gcgggcgctg gtcgagtagg tgtgggccgt ccggtccatt
     1381 gtcgtcgctg tcaggcccct tcgtgcagca cagccgcaca ggcgcacggc tgcccaaagg
     1441 gggtcccaca cggcgccctc ctccacgggg accgcgcctg gtcaggtcga cggcgattcc
     1501 tcgatcgcgc gcggctcatc acccacctcg accgggcgcg gctcagcgaa cgcctcgagg
     1561 ctgttgcggg ccaccacgat cgccagccag aagccgagga tcgccgccac cgggtggaat
     1621 gccgcggcga tcggcgacga atcgcgcagg gtggccagga ttggcacaac gaagatcacc
     1681 gccgccagcg ccagcgccca cagccagtgg ctgcgcccgg cgcgcgacag ggccgcggcc
     1741 agcagcacca cgatcggcgc caggtgcagg atccagccca gggttacgtg cagctcgatc
     1801 ccgacgttgt ccgcgaacag ccccagaccg atgaagaaga cctggaccac cacccccacc
     1861 aggaacgccc aggccaggat gacgtacgcg tagcgagccg ctcgggtcac gatgggctac
     1921 ctcccgtttc ctcacgtggc gcccgccaca acatcaccag gccggcggcg gcagcaatgg
     1981 ccgcgccgcc gaacactatc aggctgcccc accgtacggc cgactcgctg accaggccgg
     2041 ggacgaaggt gttgaaggcg aacacggagc caacgcctcc caccactgac agggcaatcc
     2101 ccgccaggta ggcccacacc tcgcggttgc gcatcccttc cgccacctcg ccggccatca
     2161 gcccgcaaat gaccagcccg gcaccgatga tggcggtgag cgccacccag cccaggtcgg
     2221 tcaccccgcc gaggcccagc agcaccagga acccgaatgt cccgatcccg ccgcgcagcc
     2281 aggccttgga gtagccgcgg aagatgatca ccggcaccgc caccagcgcg tccggaaccg
     2341 attctggctg gtcctcctgg gtcttggcct tccgcgcctc gagttccttg cgttgctcct
     2401 cgacttgtgc ctcatcggcg ccgcgcacgg tcttgcactt gtagcagcgc tcgtcctggg
     2461 cgcggttcca cttccagcag accttgcaca cccacccatc cggtgcggtc acccggttcc
     2521 ctccgcgatt ggtccagtgg cgcagattat cccgcgactc gtccagcccc tcagtcctcg
     2581 gcgccgtcac cgaaccggag ccggccgcgg gcacggccga gcatacgcgg caagtacggc
     2641 agcagcagtg ccgctgcggc agtcagcagg aggatggcgg atatgggccg cgtgaaaaag
     2701 acgctcaggt cgccctggct gatttgtagg gcgcgccgga actgcgcctc gagcatgggt
     2761 gccaggatga cgcccaggat catggggcca accgggaagt catagcggcg catgaagaac
     2821 cccaggacgc cgattgcgta catgacgagg acgtcaacga gcgagttggc cagcgagtac
     2881 acgccgaggg tcgcgaacac caggatggat gcgtacagca acgctcgcgg gatagccagg
     2941 atcttgaccc acagccggat gagcggcagg ttgaggagca gcagcatgaa gttgcccagg
     3001 tacaggctgg cgatcaacgc ccagacgagg tcgggcgagt tctgaaagag cagagggccg
     3061 ggctgtaggt tgtagatctg gaatgcggcc agcagaatgg ccgcggtcgc cgaggttggt
     3121 ataccgagcg tgagcagggg caccagcacg cccgagaacg cggcgttgtt cgcggcctcc
     3181 ggcccggcca cgccctcgat cgcgcccttt ccccagggcg aacgtggcgc cagacgcttc
     3241 tcgatgttgt acgagaggaa ggtcggtatc tcggcaccac cggccggaag ggcaccgata
     3301 gggaacccga tcgccgaccc acgaagccat ggcttccatg atcgacccag ctcctcccga
     3361 ctcatgagca ggcggccacg caccggcacg atctcctggc ggtcgtgccg catcctcgac
     3421 gcgacgtaca gcgcctcccc gaccgcgaac agcccgaccg ccacgaccac gatgtccagt
     3481 ccattgagga gctgcggcac gccgaacgtg aagcgctcct ggccggtcaa gttgtcgatg
     3541 ccgatcagcc cgatgaacag gcccacgaac aggctgatca tgccgcgcag cagcgagcgc
     3601 ccgatgaggg cggtgacgct gacgaaggcc accaccatca gcccgacgta gtctgccggc
     3661 tggaagccgc gggcgaacgc cgctaccact ggggccagga atgtgatggc cagcgtcgag
     3721 atggtgcctg cgacaaacga gcccaccgca gccgtcgcca gcgccgcccc gcccctcccc
     3781 tgcttcgcca tctggaagcc ctcgatggtc gtcaccaccg atgccgactc gccgggcgtg
     3841 ttcagcagga tggaggtcgt ggaccctccg tacatgccgc cgtagtagat gccggcgaac
     3901 atgatgaacg cgcctgtcgg ttcgaagttg tacgtgatcg gcagcaggag ggccacggtc
     3961 aacgccgggc cgatgccggg cagcacgccc accagcgtgc cgagcaggac gccggtcgcc
     4021 gcgaacagca ggttctcgac cgagagcgcg gtggcgaatc cgttggccag gtcgcccagc
     4081 acgtccatgg tgcgcgctcc ctacggcagg acgaagtcca ggacgcccgg tggcagccgg
     4141 acaccaagga actgggtgaa cccgaagtag actgccagcg cgaggccgac cccggcgatc
     4201 acgtcgcgca acagcgcccg gctgccgaga gtgcgggcga cggcgggcag gaagaggccg
     4261 gtggccagcg ggtagccgag gcctgggata tccagcggac ccagccgaac accgtcgagt
     4321 gccagggcgt aggcgacgag caccgcacca accaagccga ccgtcggcca gtgcgtggcg
     4381 ctctcctcct cggcagcctg gcgggcgagg tcttcgtccg gccggacggt ggttcggacc
     4441 gcgaacagcg cacccagcac caggagcccg gtggcaacaa ccagcgggat ggtcgccgga
     4501 ccaatgacgc tgtacccgcc gcctctcgcg atcccgagtg cggagacgac taggccgatc
     4561 gcccccagca ccaggaccgc ggcaacgaac cggggaccga gcaggcctcg gccgggatcg
     4621 gccatcggct cagccgccgg tcag
//
LOCUS       DATACA010000043         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1371910, whole genome shotgun sequence.
ACCESSION   DATACA010000043 DATACA010000000
VERSION     DATACA010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371910"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..333
                     /locus_tag="VHK28_00885"
     CDS             70..333
                     /locus_tag="VHK28_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140813.1"
                     /translation="MNTSSPKLWVLGWTGATLGASLALLGLLTPGELSGLLGGYGLLT
                     LGSALYLVAGLKLRETLARRRGMTRATVNASSHGPISTTASRP"
     gene            complement(361..1251)
                     /locus_tag="VHK28_00890"
     CDS             complement(361..1251)
                     /locus_tag="VHK28_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2140814.1"
                     /translation="MPQFVSEPLSERVGVPVVVKTETANPIGSFKGRGTWLAVSELAA
                     AGEVGEGSGVVLASAGNFGQGVAYAGRALGVRVIVFAAGNANATKVEAMRRLGAEVRL
                     VGEDFDAARAAAAAFAADHSWRLLIDGQDPWITIGAGTLAVELTEAISAGELPAFEAI
                     YVPVGNGALIGGVGRWLHAALPSVRVIGVQADQAPAMTLSWRERRPIETERADTVADG
                     IAARVPVPEALDIMLDAVDEMQLVSEDAILAAQRELEAALPFTVEPSAGATWAAVTDR
                     RKPDGPVAMVLTGGNVDRPG"
     gene            complement(1324..>1989)
                     /locus_tag="VHK28_00895"
     CDS             complement(1324..>1989)
                     /locus_tag="VHK28_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2140815.1"
                     /translation="NAGKSTLLNAMSDADVFAADMLFATLDPTSRAVELPSGQRVILT
                     DTVGFINKLPHDLVDAFRATLEEVVRADLLLEVVDAADPDFISQQQAVQTVLDELGAG
                     DKPRITAFNKVDLLDADLRTGPMPSSPQAVFVSALTGEGLDELRARIVDALRGGMEAV
                     DEVVPWARGELVARARAAGEVAEDYTQGGIRLSGHLPAGMAAEVRNAAPRRGRSGRGR
                     SAV"
BASE COUNT          281 a          750 c          677 g          281 t
ORIGIN      
        1 gacctgctgg aggtaccggg taccgatggg tcatcgtgga ctgcatcatg ggctcgtacg
       61 ctcgcggtca tgaacacctc cagccccaag ctctgggtcc tcggctggac cggcgcgacc
      121 ctcggtgcct cgctggcgct cctcggcctg ctgacccctg gcgagctctc agggctcctg
      181 ggcggctacg ggctgctgac gctcggctcg gcgctctacc tggtcgccgg cctgaagctg
      241 cgagagacgc tggcgcggcg gcgcgggatg acccgcgcga ccgtcaacgc gagctcccac
      301 ggcccgatca gcaccaccgc cagccgcccg tagcctccga tccaggcgac gcgtccggcc
      361 tcagccggga cggtcgacgt tgccgccggt gagcaccatg gcgaccggac cgtccggttt
      421 cctgcggtcg gtcaccgccg cccacgtggc cccggccgag ggctcaacgg tgaacggcag
      481 cgcagcctcc agctcgcgct gagcggccag gatggcatcc tcgctgacca gctgcatctc
      541 gtcgaccgcg tcgagcatga tgtcaagcgc ctcgggcacc ggcacgcggg cggcgatgcc
      601 gtcggccacg gtatcggccc gttcggtctc gatggggcga cgttcgcgcc atgagagcgt
      661 catggccggc gcctgatcgg cctggacccc aatgacccgc accgacggca gcgcggcgtg
      721 cagccagcgt cccacgccac cgatcagggc tccgttgccg accggcacat agatggcctc
      781 gaacgccggc agttcgccgg cgctgatcgc ctcggtgagc tccacggcca gcgtgccggc
      841 accgatcgtg atccacgggt cctgcccgtc gatcagcagt cgccatgaat ggtcggcggc
      901 gaaggcggcc gccgcggccc gcgcggcatc gaagtcctcc cccaccaggc gaacctcggc
      961 gcccagccgc cgcatggcct ccaccttggt cgcgttggcg ttcccggccg cgaagacgat
     1021 gacccggacg cccagcgccc ggccggcgta ggcaaccccc tgcccgaagt tgccggccga
     1081 cgccagcacc acacccgatc cctcccccac ctcgccggcg gccgccagct cgctgaccgc
     1141 cagccaggtg ccgcgtcctt tgaacgagcc gatcgggttg gccgtctcgg tctttaccac
     1201 caccggcacc ccgacgcgct cgctcagcgg ctcggagacg aactgcggca tgttgcggaa
     1261 ggctgccggg acgcgggaat gggcagcctt gatcgcttgc ggcgaaacgg ggtccaccgg
     1321 gggtcagacg gcggagcggc cgcggccgga gcggccccgg cgcggcgccg cgttgcgcac
     1381 ctcggcggcc atcccggccg gcagatgccc agagaggcga atgccgccct gggtgtagtc
     1441 ctcggccacc tcgcccgctg cccgggcgcg ggccacgagc tcaccgcgcg cccagggcac
     1501 gacctcgtcc acggcctcca tcccgccgcg cagggcatcc acgatgcgcg cccgcagctc
     1561 gtccaacccc tcgccggtca gggcgctgac gaagaccgcc tgcgggctgg acggcatcgg
     1621 tccggtgcgc aggtcggcat ccagcaggtc gaccttgttg aaggcggtga tgcgtggctt
     1681 gtcaccggcc cccagctcat ccagaacggt ctgcactgcc tgctgctgac tgatgaagtc
     1741 cgggtcggcg gcatccacca cctcgagcag caggtctgca cgcaccacct cctccagcgt
     1801 ggcgcggaag gcatcgacca agtcatgggg cagcttgttg atgaagccca cggtatcggt
     1861 caggatcacg cgctggcccg atggcagctc gaccgcccgg ctggtggggt cgagggtggc
     1921 gaacagcatg tcggcggcga acacgtccgc gtcgctcatg gcgttgagca gcgtgctctt
     1981 gccggcgtt
//
LOCUS       DATACA010000044         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2416570, whole genome shotgun sequence.
ACCESSION   DATACA010000044 DATACA010000000
VERSION     DATACA010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416570"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="otsB"
                     /locus_tag="VHK28_00900"
     CDS             complement(<1..712)
                     /gene="otsB"
                     /locus_tag="VHK28_00900"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX2140816.1"
                     /translation="MGPSATALARSALEAAPAGLLTDFDGTLSPIMREPDAARPVAGA
                     VEALEALAARLAVVGVVSGRAAADARRLMGTDRLLVIGNHGLEWLEPGAARAETGVGE
                     GVRRAVALAVEAVPSEQGVEVERKGLSATVHVRKSLEPDAARERVRAALEAAAIPGIV
                     LRPGRMSLELRPSAAGDKGTAVREVVARYGLRGLLVLGDDVTDLDMFRAADELRQAGR
                     LRAAIIGVGAAGEAPPEVE"
     gene            761..1474
                     /locus_tag="VHK28_00905"
     CDS             761..1474
                     /locus_tag="VHK28_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEX2140817.1"
                     /translation="MRRLSPGMVVPLLLAVAGVALIMVGQLDLDQPPGPSLPGIPDPS
                     TPIAVASPTLSAEPSEADSPSPSTSPDPTPSPTPIPADWVAVQIEIESVGINVPVRQA
                     IDAQHCDFPPSDAAYVLCGGSQPGRDTNSYIFAHAVEHLFKPLWNVQLGHEVEILMSD
                     GAVLRYQVTEVRPNISCPDSRAPGHPNPPLALLYAPPGCEDAAQWTQPTDYERLTLQT
                     SQGFNRNWGELIIVAEPMA"
     gene            complement(1674..3242)
                     /locus_tag="VHK28_00910"
     CDS             complement(1674..3242)
                     /locus_tag="VHK28_00910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2140818.1"
                     /translation="MSERPSVAMHPAAAWAVLVVASLGVFLGGAELMVVAIALPDIVA
                     DFGGWADLARASWIVNAYLLAYIVAMPLAGRAADLWGARRLYVVTLVLFSIGSLGAGL
                     SRFAGPESGMDWLIASRVVQGFGGGALVPLSMALASHLFSGRARAAALGVEGAATFVG
                     MAVGPAYGAWVLLNFNLPLPGVDMATWQWIFFLNVPAGIITLLLIYVVAGGVETPRAS
                     GRLDIGGALLITVALLAGLASLTLAGARGWGDPLILGGFAVSAVTIGLFVWLELRLRN
                     PLVHPRLFADRSFSAANAVSLLTGYTLATAIIGGPVFVNRVLAGGADQSAAALTALTV
                     AIAGGAVAGGLITAVVGERITTVLGVALTVVALWLATGWGTDTNLAVLVRDLAVFGAG
                     FGLTVSPRATAAVEAAGASAYGVASAMLQITRTLGMSVGLALLTSIGQNRIDELNELI
                     NDPVRRDALVVELGRPEFVGVDPRASLALVDVLEEWSRGQAAEVLQLVLLIALGVAVI
                     TLVPALLVGRRGER"
     gene            complement(3239..>3628)
                     /locus_tag="VHK28_00915"
     CDS             complement(3239..>3628)
                     /locus_tag="VHK28_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140819.1"
                     /translation="GSSKERSAPVSRAAAEPLVAGRPVAFGVALLAVLAAGLACVSLG
                     LALGAVLPRLPAALLTLAVSAAALLTGALRSFELPMSPFAGMAVLAHLDDAGAPLGDA
                     LRSGGTALALSAVLLVLAAALLERVDL"
BASE COUNT          570 a         1399 c         1243 g          416 t
ORIGIN      
        1 cctcaacctc cggcggggcc tctcccgcgg cgcctacgcc gatgatcgcc gcccgcagcc
       61 gcccggcctg ccgcagttcg tcggcggcgc ggaacatgtc caggtcggtc acgtcgtcgc
      121 ccaggaccag cagtccccgc aggccgtatc gcgccacgac ctcccgtacc gccgttccct
      181 tgtcgccggc ggcggacggc cgaagctcga gcgacatgcg ccccggccgc agcacaattc
      241 cagggatggc cgccgcctcg agcgcggcgc gcacccgctc ccgcgcggcg tcaggctcaa
      301 gggacttgcg gacgtgcacg gtggccgaca ggcccttgcg ctcaacttcc acgccctgct
      361 cgctgggcac cgcctcgacc gccagcgcca cggcccggcg aacgccctcc cccaccccgg
      421 tttccgcccg cgccgcgccg ggctccagcc actccagccc gtggttgccg atgaccagca
      481 gccgatcggt gcccatcagg cgtcgcgcat cggctgcggc acggccgctg accacgccga
      541 ccaccgccag gcgggcggcc agcgcctcga gtgcctccac ggcaccggcc accgggcgcg
      601 cggcatccgg ctcgcgcatg atcggtgaca gggtcccgtc gaaatcggtc aggaggccgg
      661 cgggcgcggc ctcgagcgct gatcgggcaa gggcggtggc ggagggtccc atcgctcccg
      721 tcagggggtt cggcggaacg ttggtagact ccgccccgtc atgcgtcgcc tgtctcccgg
      781 catggtcgtg ccgctcctgc tcgccgtggc tggggtggcg ctcattatgg tcggccagct
      841 ggacctggac cagccgccgg gcccgagcct gcccggcatc ccggacccgt ccacgcccat
      901 cgcggtcgcc tcgccgaccc tcagtgccga gccgtcggag gcagactcgc cgagccccag
      961 cacctccccg gacccgacgc cgtcgcccac gcccatcccg gccgactggg tggcggtcca
     1021 gatcgagatc gagtcggtgg gcatcaacgt gcctgtgagg caggcaatcg acgcgcagca
     1081 ctgcgacttt ccgccgtccg acgcggccta cgttttgtgc ggcggctcac agcccggccg
     1141 cgacacgaac tcgtacatct tcgcccacgc ggtcgagcac ctcttcaagc cactgtggaa
     1201 cgtgcagctg gggcacgagg tcgagatcct gatgagcgat ggcgcggtgc tccgctacca
     1261 ggtcaccgag gtgcggccca acatcagctg cccggactcg cgcgcgccgg ggcatcccaa
     1321 cccgccgctg gccctgctct acgcgccgcc cggctgcgag gatgcagcgc agtggacgca
     1381 gcccaccgac tacgagcgcc tcacgctgca gaccagccag ggcttcaacc gcaactgggg
     1441 cgagctcatc atcgtggccg agcctatggc ctaggcgggc cgccgccgat gtagcgggca
     1501 gcctttcacg cggggccagt cgttggtgtc aatctgccga tatagcgggc agcgttgagc
     1561 acgaaccgac aagatgcggc tccctcacgc tcacgcctgc ccgatatatg cgcagttccg
     1621 ctcatggcgg gactcgcgcg tgaactcctg cccgatggat cgggcagcgc ggcctatcgc
     1681 tcgccccggc ggcccactag cagcgccggc accagggtga tcaccgccac cccgagggcg
     1741 atgagcagca ccagctgcag cacctcggcc gcctggcccc gcgaccactc ttctagcacg
     1801 tccaccagcg cgaggctggc gcgcggatcc acgcccacga actcgggccg gccgagctcg
     1861 accaccagcg cgtcacggcg caccgggtcg ttgatcagct cgttcagctc gtcgatccgg
     1921 ttctgcccga tgctggtcag cagggccagc ccaacgctca tgcccagcgt gcgcgtgatc
     1981 tgcagcatgg cgctggccac cccgtaggcc gaggccccgg cggcctccac cgccgccgtg
     2041 gcgcgcggcg agacggtcag cccgaagccg gcgccaaaga cggccaggtc gcggaccagc
     2101 acggccaggt tggtatcggt cccccatccg gtcgcaagcc agagcgcgac cacggtgagg
     2161 gcgacgccca gcacggtcgt gatccgctcg cccaccacgg ccgtgatgag gccgccggcc
     2221 accgctcccc cggcgatggc caccgtcagg gcggtcagcg ccgcggcgga ctgatcggcg
     2281 ccaccggcca gcacgcggtt gacgaacacc ggtccaccga tgatggcggt ggccagcgtg
     2341 tacccggtca gcaggctgac ggcatttgcc gccgagaagc tgcggtcagc gaacagccgc
     2401 gggtggacca gcgggttgcg caagcgcagc tccagccaga cgaagaggcc gatggtgacg
     2461 gccgagacag cgaagccgcc caggatgagc gggtcgcccc agccgcgggc gccggccagg
     2521 gtcagcgagg ccagcccggc gagcagcgcg acggtgatga gcagcgcgcc accgatgtcc
     2581 aggcgcccgg aggcccgcgg cgtctccacc ccacccgcca ccacgtagat cagcagcagg
     2641 gtgatgatgc cggcgggcac gttcaggaag aagatccact gccaggtggc catgtcgacg
     2701 cccgggagcg gcaggttgaa gttgagcagc acccaggcgc cgtacgcggg cccgaccgcc
     2761 atgcccacga acgtggccgc gccctccacg cccagggccg cggcgcgggc gcgcccgctg
     2821 aacaggtggc tggccagcgc catggagaga ggcaccagcg cgccgccgcc gaagccctgc
     2881 accacgcgcg aggcgatcag ccagtccatc cccgattccg gcccggcgaa gcgcgacagc
     2941 ccggccccca ggctaccgat actgaacagg acgagcgtca ccacgtacag gcgccgcgcg
     3001 ccccacaggt cggcggcgcg cccggccagg ggcatggcca cgatgtaggc cagcagatag
     3061 gcgttgacga tccacgaggc gcgggccagg tccgcccagc cgccgaagtc ggccacgatg
     3121 tccggcaggg cgatggccac caccatcagc tccgcgcctc ccaggaacac gcccagggac
     3181 gcgacgacca gcactgccca ggcggcggcc gggtgcatgg ccacgctcgg ccgctcgctc
     3241 acaggtcgac gcgttccagc agcgcggcag ccagcaccag gagcacggcc gacagggcga
     3301 gggccgtgcc gccggatcgc agtgcatcgc cgaggggcgc cccggcatcg tccaggtggg
     3361 ccagaactgc catgccggcg aacggcgaca tgggcagctc gaaggagcgc agcgcgccgg
     3421 tcagcagggc ggccgcggag acggcgagcg tgagcagcgc ggcaggcaat cgcggcagga
     3481 ccgcaccaag ggccagtccc agtgacacgc aggccaggcc ggcggccagc acggccagca
     3541 gtgccacgcc gaacgccacc gggcgaccgg caaccagcgg ctcagcggcc gccctgctga
     3601 ccggcgcgct gcgctccttc gagctgcc
//
LOCUS       DATACA010000045         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_247575, whole genome shotgun sequence.
ACCESSION   DATACA010000045 DATACA010000000
VERSION     DATACA010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247575"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..704
                     /locus_tag="VHK28_00920"
     CDS             24..704
                     /locus_tag="VHK28_00920"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEX2140820.1"
                     /translation="MSPERFVHAWLLRLDPAWRRLPSTERRDDLDAFCAAMDRARDRV
                     VQHEYSTLGLRAEGDLLLWRMAERLEDLEESAAALLSSGIGCWFTTAVSMTGLTRPSQ
                     YVKRPTTQEQSLFAGERSRYLVVYPFVKSTEWYLAPADARQHVMREHMRVGHRYPQVR
                     QLLAYSFGLDDQEFVVAYETDDLVGFQDLVRELRETESRRSTVRDTPILTGIHRPLTE
                     ILTMLSPA"
     gene            701..1954
                     /gene="hemL"
                     /locus_tag="VHK28_00925"
     CDS             701..1954
                     /gene="hemL"
                     /locus_tag="VHK28_00925"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX2140821.1"
                     /translation="MSPVATSRSDELMERAERLLPGGVSSPVRAFGAVGGKPRVIARG
                     AGAHVWDVDGHELVDYVGSWGPMILGHAHPSVVDAVMRVVRDGTSFGAPTSVELELAE
                     EIATAMPAIERLRFVNSGTEAAMSAVRLARAYTGRDRVLKMIGGYHGHVDALLVRAGS
                     GAHGLPASAGVTHGAARDTVVVPYNDLDAAAEALREGNVAAVIVEPVAANMGVMPPAP
                     GYLDGLRELTQRYATLLIFDEVITGFRVGRGGGQERYGISPDLTLLGKIIGGGLPVGA
                     YGGRADVMELVAPLGPVYQAGTLSGNPLAMAAGVATLRALDGDAYARLERAGAYLEEG
                     LTAAAGRAGVTARVARVASLLTVFLEHEAAFARFFHAMLDAGVMLPPSQQEAWFISTA
                     HQSSDLDFTLAAAEAAFATAGEPHA"
     gene            1947..>2217
                     /locus_tag="VHK28_00930"
     CDS             1947..>2217
                     /locus_tag="VHK28_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX2140822.1"
                     /translation="MLDLPTRSMTGEERFMAALHHRQPDATPVWFMRQAGRALPAYRA
                     LRERHDILTLAKTPELCAEVTVMPVDEFGVDAAVMFADIMLPLEPM"
BASE COUNT          323 a          732 c          801 g          361 t
ORIGIN      
        1 tggctgcgcg aggatgccgg ctgatgagtc cggagcgctt cgtccacgcc tggctgctca
       61 ggctcgatcc cgcctggcgg cggctcccgt cgaccgagcg ccgcgacgac ctggatgcct
      121 tttgcgccgc gatggaccga gcgcgagatc gcgtggtgca gcacgagtac tcgacactcg
      181 ggctgcgcgc cgagggcgac ctcctcctgt ggcgcatggc ggaacggctg gaagacctgg
      241 aagagtcggc cgccgcgctg ctcagcagcg gcatcggatg ctggttcacg accgccgtgt
      301 cgatgaccgg cctcaccagg ccgagccagt acgtgaagcg gccgaccacg caggagcaga
      361 gcctgttcgc cggagagagg tcgcgctacc tggtggtcta tccgttcgtg aagagtaccg
      421 agtggtacct ggcgccggcc gacgcccgcc agcacgtgat gcgcgagcac atgcgcgttg
      481 gccaccgata tccgcaggtg cgccagctgc tggcgtactc gttcgggctg gacgaccagg
      541 agttcgtggt cgcctatgaa accgatgacc tggtcgggtt ccaggacctg gtccgcgagc
      601 tgcgcgagac cgagagccgg cgcagcacgg tccgcgacac gcccatcctg accggcatcc
      661 atcgcccgct caccgagatc ctcaccatgc tgtcgccggc atgagtccgg tggcgaccag
      721 ccggtcggac gagctgatgg aacgtgccga gcgactgctg cccggcggcg tgagctcccc
      781 cgtgcgcgcc tttggcgccg tgggcgggaa gccacgggtg atcgcccgcg gcgcgggtgc
      841 gcatgtctgg gacgttgacg gtcacgagct ggtcgattac gtcggctcct gggggccgat
      901 gatcctgggc cacgctcacc cgtccgtggt ggacgccgtg atgcgcgtcg tgcgggacgg
      961 aacgtcgttt ggcgcgccga cctcggtcga gctggagctt gcggaggaga tcgccaccgc
     1021 gatgccggcc atcgagcggc tgcgattcgt gaacagtggg acggaagccg caatgtcggc
     1081 ggtgcgcctg gcacgtgcgt acactgggcg cgaccgcgtg ctgaagatga tcggcggcta
     1141 ccacggacac gttgatgcgt tgctggtgcg cgctggctct ggcgcacatg ggctgccggc
     1201 gagcgccggc gtcactcacg gggctgcacg ggacaccgtg gtggtcccct acaacgacct
     1261 ggacgcggcg gccgaggcgc tgcgggaggg gaacgtcgcc gccgtgattg tcgaaccggt
     1321 cgcggcgaac atgggcgtta tgcccccagc gcccggatat ttggacgggc tgcgggagct
     1381 gacgcaacgg tacgcaacgc tgctcatctt cgacgaggtg atcaccggct ttcgggtcgg
     1441 gcggggcggc ggccaggagc gatacggcat cagtcccgat ctcacattgc ttggcaagat
     1501 catcggcgga ggcctgcccg tcggcgcgta tggcggccgg gcggacgtca tggagctggt
     1561 tgcgccactc ggcccggtct accaggcggg gaccctctcc ggaaacccgc tggccatggc
     1621 cgccggggtg gccacgctcc gcgcgctgga tggggacgca tatgcccggt tggagcgtgc
     1681 aggcgcctac ctggaggagg gactgactgc cgcggccgga cgcgccgggg ttaccgctcg
     1741 cgtcgcacga gtcgcctcgc tgctgaccgt cttcctcgag cacgaggcgg cattcgcacg
     1801 cttcttccac gccatgctgg atgcgggcgt gatgctgccg ccgtcccagc aggaagcatg
     1861 gttcatctcg acggcgcatc agtcgtccga tctcgatttc accctcgcgg ccgcggaggc
     1921 cgcgttcgct actgcaggag aaccccatgc ttgacctccc gacccgatcc atgaccggag
     1981 aggagcgctt tatggccgcg ctgcaccatc ggcagccgga tgccacgccg gtgtggttca
     2041 tgcggcaggc gggtcgcgcg ttgccggcgt accgtgcgct ccgcgagcgt cacgacatcc
     2101 tgaccctggc caagacgccg gagctgtgcg ccgaggtcac cgtcatgccg gtggacgagt
     2161 tcggcgtgga tgcggccgtg atgttcgccg acatcatgct tcccctcgag cccatgg
//
LOCUS       DATACA010000046         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2175782, whole genome shotgun sequence.
ACCESSION   DATACA010000046 DATACA010000000
VERSION     DATACA010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175782"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="VHK28_00935"
     CDS             <1..1121
                     /locus_tag="VHK28_00935"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2140823.1"
                     /translation="PGDSPAPSTTIGPPSVPAPPSVIPSVEPIGITHAGDGSGRLYVN
                     GRTGLVQIVEPDGTVRGEPFVDISDRVQAGGERGLLGLVFHPDFERNRRLFVHYSRAG
                     DGATVVSELRAQDSLRADAGSERVLLSAAQPFPNHNGGQLAFGPDGYLYIGLGDGGGG
                     GDPFDNAQNLDVILGKVLRIDVDGRATGGRQYAIPRDNPFAADGSQPGAGAPEVWASG
                     LRNPWSFSFDREGGDLYIADVGQNAWEEVNRQPGDSRGGENYGWDVMEGRHCYQDGCD
                     QTGYVKPIAQYGHDLGCSVTGGHVYRGRAQPQLNGVYVFADYCSGLVFTLQVDEGTRT
                     PKVVLESGLRVTAIGTDEDGEIYLADLAGGAIHRVLVD"
     gene            complement(1128..1799)
                     /locus_tag="VHK28_00940"
     CDS             complement(1128..1799)
                     /locus_tag="VHK28_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEX2140824.1"
                     /translation="MGPFPAPITITEVRRSRGGAGRSAPRVDEMSDVDLVSLLIGSAA
                     DAAAILAEVGGLPGLAQDGHGRLADLAPPIPRAARRLGAALELARRMAEAGCRPGWRI
                     RSPADVGERLRPAMRHLEQEELRTLLLNTKNVVVGARTVYVGNLAGSSVRVGEVFRDA
                     VRSQAAAVVVVHNHPSGDPTPSADDLHISAELAQAGRLLDIELLDHVVVGAEGWVSIR
                     ALGGL"
     gene            complement(1869..2864)
                     /locus_tag="VHK28_00945"
     CDS             complement(1869..2864)
                     /locus_tag="VHK28_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX2140825.1"
                     /translation="MVGAPGTEFEPPPSAEPSRPVPRYRLVQLLSILLIVGGLLAVAT
                     AVGLSLDAVPGDGPQGGVAGSGQLLLGGGLALVAGLLLNVGRALAVRRFLPPERYRGP
                     SILVLLLLAGVISTAASLLVADDLVALGGDATPSVAGSLLLLTVTQAALLVVSAAFVF
                     WPRALAGVRLVPSRGVMRSVALGLALALPVWFGAQVVNVVVSLCLQALGLEFDPGITD
                     AAMALVDPLVLVLALVVVAPVAEETFFRGVVYNAWEREYGSRLAVGGSALLFALIHGS
                     IFVLVPIVLLGVLLAVFYRRTRSLPAAIALHAGFNAISLALALLVRYEVIRVPAT"
     gene            complement(2877..3473)
                     /locus_tag="VHK28_00950"
     CDS             complement(2877..3473)
                     /locus_tag="VHK28_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2140826.1"
                     /translation="MPDGLPDPGQIWNDFINNPIVVLAFQLIVIYVVMLWLGTAYWAF
                     RDMQARTENPILPYFSAALIIFFTPLLFVFAVVLYLIVRPRETLAEVYERSLAEESLL
                     AEVEKNQLCPVCRDRVQADWLVCPNCRTRLHRVCPSCNRLAQPEWPLCAFCGHEFQRA
                     PAARPVPRASEWPAQPAAGTERGERQPEPGGLRRPASS"
     gene            complement(3466..4137)
                     /gene="purN"
                     /locus_tag="VHK28_00955"
     CDS             complement(3466..4137)
                     /gene="purN"
                     /locus_tag="VHK28_00955"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX2140827.1"
                     /translation="MRVGFLVSGRGSNLAAVLQAIALGELPGVEPVVVISNRDGVSAL
                     SVADRYGVPARVLARSAFASRDERDAAIGRALAAGGADLALLAGYDQLLGRSFFDAFG
                     GRTVNIHPSLLPRHGGKGMVGLAVHAAVLAAGDEETGVTIHDVTAELDSGPAIATCRV
                     PVLPGDEAATLAARVLREEHRLLVTTLRRLASLPEPAGPSASMAALPAPARLGGDAQR
                     NPPHA"
     gene            complement(4142..>4454)
                     /locus_tag="VHK28_00960"
     CDS             complement(4142..>4454)
                     /locus_tag="VHK28_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX2140828.1"
                     /translation="SDLLALAHLTGGGWVDNIPRTLPAGLGVEVESGSWPVPRIFSLI
                     QERGDVPDEEMVRTFNLGIGLTAAIRPEMVEVVRSALPEVVRVGTVVPVAEGRPRVAF
                     V"
BASE COUNT          729 a         1626 c         1574 g          525 t
ORIGIN      
        1 cgcctggcga cagccccgcg ccgagcacga ccatcgggcc gccatcggtg ccagccccgc
       61 cgagcgtcat tccttcggtg gagccgatcg gcatcacaca cgccggcgac ggcagcgggc
      121 gcctgtacgt gaatgggcgc accgggctgg tgcagatcgt ggagccggac ggcacggtgc
      181 gcggcgaacc gttcgtcgac atctcggatc gcgtccaggc cggcggcgag cgaggcctgc
      241 tgggcctcgt cttccacccc gacttcgagc gcaaccggag gctcttcgtc cactacagcc
      301 gcgcgggcga cggggcgacg gtggtatctg agctgcgagc gcaggattca ctgcgcgccg
      361 acgcaggcag cgagcgggtc ctgctcagcg ccgcccagcc cttccccaac cacaacggcg
      421 gccagctggc gttcggcccg gacggctacc tgtacatcgg tttgggcgac ggcggcggtg
      481 gtggtgaccc gttcgacaac gcccagaacc tcgacgtgat cctcggcaag gtgctgcgca
      541 tcgacgtgga cggcagggcg accggcgggc gccagtacgc gatcccgcgg gacaacccat
      601 tcgccgccga cggcagccag cccggcgccg gggcgcccga ggtgtgggcc tccggcctgc
      661 gcaacccgtg gagcttcagc ttcgatcgcg agggcggaga cctgtacatc gcggacgtcg
      721 gccagaacgc ctgggaggag gtcaaccgcc agccgggcga ctcgcggggc ggcgagaact
      781 atggctggga cgtgatggaa ggccgccact gctaccagga cggctgcgac cagaccggct
      841 acgtcaagcc gatcgcccag tacggccacg acctgggctg ctcggtcacc ggcgggcacg
      901 tctatcgcgg ccgggcgcag ccgcagctca acggggtcta cgtcttcgcc gactactgct
      961 ccggcctggt cttcacgctc caggtggacg agggcaccag aacacccaag gtggtgctcg
     1021 agagcgggtt gcgcgtgacc gccatcggca ccgatgagga cggcgagatc tacctggccg
     1081 acctggccgg cggcgccatc catcgcgtcc tggtcgactg accgcgctca caggccaccc
     1141 agggcccgaa tgctgaccca tccctcggcg ccgaccacca cgtgatcgag cagctcgatg
     1201 tccagcagtc gaccggcctg cgccagctcg gcgctgatgt gcaggtcgtc ggccgacggc
     1261 gtggggtcac cggacggatg gttgtggacc accaccaccg ccgccgcctg gctgcgcacc
     1321 gcgtcgcgga acacctcccc aacccggacc gacgagccgg cgaggttgcc gacgtacacg
     1381 gtccgggcgc cgaccaccac gttcttggtg ttcagcagca gcgttcgcag ctcctcctgc
     1441 tcgaggtggc gcatggcggg gcgcagccgc tcgccgacgt cggccggcga gcggatcctc
     1501 cagcccgggc ggcagccggc ctccgccatg cgccgtgcca gctccagcgc ggcaccaagc
     1561 cgtcgcgccg ccctcgggat tggtggcgcc agatcggcaa gccgcccgtg tccatcctgg
     1621 gccagcccag gcagcccgcc gacctcggcc aggatggcgg cggcgtccgc cgcggagccg
     1681 atgagcaggc tgaccaggtc gacgtcgctc atctcgtcaa cgcgcggcgc tgagcgcccg
     1741 gcacctcccc gggaacggcg gacctcggtg atggtgatcg gggcggggaa cggacccatg
     1801 ccccgaggct agctgtgagg gcctcaccgg ccgcttatca gcggcttatc ggttcttgtg
     1861 gccgccgctc aggtggccgg cacccgaatc acttcgtagc gcaccagcag ggccagcgca
     1921 aggctgatgg cgttgaagcc ggcatgcagg gcgattgccg ccggcaggga gcgagtccgc
     1981 cggtagaaga cggccagcag cacgcccagc aggacgatcg gcaccagcac gaagatggag
     2041 ccgtggatga gcgcgaagag cagcgccgag ccgcccaccg ccaggcggct gccgtactcg
     2101 cgctcccagg cgttgtagac gacgccgcgg aagaaggtct cctcggccac cggagccacc
     2161 accaccaggg ccagcaccag gaccagcggg tccaccaggg ccatggcggc atcggtgatc
     2221 ccggggtcga actccaggcc gagcgcctgc aggcagaggc tcaccacgac gttcaccacc
     2281 tgcgcgccga accacaccgg gagcgccagc gccaggccga gggccaccga gcgcattacg
     2341 cccctggacg gcaccaggcg taccccggcc agggcccgcg gccagaagac gaaggccgcc
     2401 gacaccacca gcagcgcggc ctgggtcacc gtcagcagca gcagagaacc ggcgaccgat
     2461 ggcgtggcat cgcccccgag cgccaccagg tcgtctgcga ccagcagcga ggcggcggtg
     2521 gagatgaccc cggccagcag cagcaggacg aggatcgagg gcccacggta gcgctccggc
     2581 ggcaggaagc gacgcaccgc cagggcgcgc cccacgttca gcaggaggcc ggcgaccagc
     2641 gccaacccac cgccgagcag gagctggccg gagccggcta cgccaccttg cgggccgtca
     2701 ccgggtacgg cgtcgaggct cagcccgacg gcggtggcca ccgccagcag gccgccgacg
     2761 atgagcagga tggagagcag ctggacgagg cggtagcggg gaaccgggcg cgacggctca
     2821 gctgacggag gaggctcgaa ctccgttccc ggggcgccga ccacgcgcgg acgcgatcag
     2881 ctggaggctg gacgccgcag gccgcccggc tcgggctgac gctcgccgcg ctcggtcccc
     2941 gccgccggct gcgccggcca ctcgctggcg cgcggcaccg gacgcgcggc gggcgcgcgc
     3001 tggaactcgt ggccgcagaa ggcgcacagc ggccattccg gctgcgccag gcggttgcag
     3061 gacgggcaga cccggtgcag ccgggtgcgg cagttggggc ataccagcca gtcggcctgg
     3121 acccgatcgc ggcacaccgg gcagagctgg ttcttctcca cctccgccag cagggactcc
     3181 tcggcgagtg agcgctcgta gacctcggcc agggtctccc gcggccgcac gatcaggtac
     3241 agcaccaccg cgaacacgaa cagcagcggg gtgaagaaga tgatcagcgc cgcgctgaag
     3301 tagggcagga tcgggttctc ggtgcgggcc tgcatgtcgc ggaacgccca gtacgccgtc
     3361 cccagccaga gcatcaccac gtagatgacg atcagctgga aggccagcac cacgatgggg
     3421 ttgttaatga agtcgttcca gatctgtccc ggatccggca ggccgtcagg catggggagg
     3481 gttcctctgt gcatcaccgc caaggcgcgc gggcgccggc agcgcggcca tgctagcaga
     3541 tggtccggcc ggctccggta gggacgccag gcggcgcaag gttgtcacca gcaggcggtg
     3601 ctcctcccgc aggacgcgcg ccgccagcgt cgcggcctcg tcccccggca gcaccggcac
     3661 acggcaggtc gcgatggccg gacccgaatc cagctcggcg gtcacgtcgt ggatggtcac
     3721 tccggtctcc tcatcgccgg ccgccaggac tgcggcgtgc accgccagcc cgaccatccc
     3781 cttccctccg tgcctgggca gcaggctggg atggatgttc acggtgcggc cgccgaacgc
     3841 gtcgaagaag gaccgcccca gcagctggtc atagccggcc agcaacgcaa gatccgcgcc
     3901 gccggcagcc agggcccggc cgatcgcagc atcgcgctcg tcccggctgg cgaaggccga
     3961 acgggccagc acccgggctg gcaccccgta gcgatcggcc acggacaggg ctgacacccc
     4021 gtcgcggttg gagatgacca ccacaggctc cacgcccggc agctcgccga gggcgatggc
     4081 ttgcagcacc gccgccaggt tcgagccacg accggagacc aggaagccga ctctcaccgc
     4141 gctagacgaa ggccacgcgc ggtcgcccct cggcgaccgg caccaccgtc cccaccctga
     4201 cgacttcggg cagggccgag cggaccactt cgaccatctc cgggcggatg gcagcggtca
     4261 ggccgatgcc caggttgaag gtgcgcacca tctcctcgtc cggcacgtcg ccgcgctcct
     4321 ggatcaggct gaagatgcgc ggcaccggcc atgagccgct ctccacctcc acgcccagcc
     4381 cggcggggag cgtgcgcggg atgttgtcca cccagccacc accggtgagg tgggcgagtg
     4441 ccagcaggtc gctc
//
LOCUS       DATACA010000047         8251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_488743, whole genome shotgun sequence.
ACCESSION   DATACA010000047 DATACA010000000
VERSION     DATACA010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488743"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1923
                     /gene="glmS"
                     /locus_tag="VHK28_00965"
     CDS             94..1923
                     /gene="glmS"
                     /locus_tag="VHK28_00965"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2140829.1"
                     /translation="MCGIVGYVGPRDAAPILLEGLRRLEYRGYDSAGIALLTTDGNVF
                     VEKKAGKLTNLTEHLNGGAPAGHPGIAHTRWATHGRPSDLNAHPHSDCSGKLALIHNG
                     IIENYAEIKDRLLAAGHKFTSETDTEVLAHLVEERYDGDLVEALRSALSDVRGAYAIG
                     VMHSDHPDRIVGARMNVPLIVGLGNGEGFLASDVPAILEHTKNVVILHEGDIADVSPD
                     GVRILQLDGSEVERDVTTIRWNIEAAEKGGFKHFTLKEIYEQPHAIQEALRGRVDASG
                     TIKLPELDAIRDKLLAAERVYLVGCGTASYAANVGAYLIQEWAQLPAFTQIGSEMRYA
                     PPPMDDRTLVISVSQSGETADTLAPQKLAAERGATIVVVTNVVGSAITREADAVCYLQ
                     AGPEIAVASTKAFVTQVLVLQLIALHLARLRGKLSDHRLRVYGQGLRELPGLAAETLK
                     IHPQVKKLARRWHDVRDVMFIGRGVGFPIAMEGALKLKELSYVHAEGYAAGELKHGPI
                     ALLDHDTPLVAIATRSATYDKVASNIAEVRAREAPVVAVATEGDEEIARYAQDVLYVP
                     ETLEQLAPAIAILPLQLLAYEVALARGTDVDQPRNLAKSVTVE"
     gene            1937..2317
                     /gene="acpS"
                     /locus_tag="VHK28_00970"
     CDS             1937..2317
                     /gene="acpS"
                     /locus_tag="VHK28_00970"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX2140830.1"
                     /translation="MGRHEVGIDLIEIERIVAILARFPDRFRERILTEAEARYCGRRA
                     ERIAGRWAAKEAISKVLGLGVRGVGWREIEILPDWAGAPQVRLHRRAAARAARLGLEE
                     VTVSISHERQMAVAVAVAHRGADG"
     gene            2310..3221
                     /locus_tag="VHK28_00975"
     CDS             2310..3221
                     /locus_tag="VHK28_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX2140831.1"
                     /translation="MADRVKTALTQRWVAERLPPRPPRAHKGTFGRLLVVAGSMEYAG
                     AALLAGLGAARTGAGLVCLATPESVGVRLMGNVPELVGLLLAEEAPGLIAPGGWRRLA
                     SEARGYDALVVGPGLGRHPSTLRRTRGFLSELERPAVVDADGLNALAESDRWWRPLRA
                     RLVLTPHPGEFARLWGGVTAQPLPDDDDARAAAAREGAVRWGQTVVLKGARTVIATPE
                     GEVIRSDVQTAALATAGSGDVLAGAIGAFLAGGCDPLAAAGCGVGVHGAAGVLAEDRI
                     GTAGVLARDLAALLPAAIRQLRGQDGS"
     gene            3218..4348
                     /gene="alr"
                     /locus_tag="VHK28_00980"
     CDS             3218..4348
                     /gene="alr"
                     /locus_tag="VHK28_00980"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2140832.1"
                     /translation="MSAPDPRSPHRAWVEIDHAALRHNLDRLRTMAGLERQVIAVVKA
                     NAYGHGAIPVARTLLDGGVERLAVATLSEAQELRVAGIEAPILVLWGLGAPEARAALA
                     ADLEAVVYEPRGLVLLEAAAADAGRRLSVHLKVDTGLGRQGAEPEEAVELAVRIARSR
                     HLQLSGTFSHLAVPGEDDAYTEVQLLRLARTLDAMRSAGIEPGLVHVSGTGGILAGAG
                     TFADAIRPGIGLYGMAPSWTDAAEHRLRPVLSLHALPLRIYDLPGGAPIGYGLRFRAE
                     RPTRIATLGIGYGDGWPRAHANNGRVLVCGRSAPVVGAISMDGITVDIGEIDDVTYDD
                     EFVLIGEQAGASISADEVAAERRTINYEVTTALRERLPRLHR"
     gene            4392..4922
                     /locus_tag="VHK28_00985"
     CDS             4392..4922
                     /locus_tag="VHK28_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX2140833.1"
                     /translation="MAVPIEIDVWQGDVAELEVDAIVVPANESLFMTSPVARAVKLRG
                     GESVERDAVAQGPIAAGTAIVTAGGTLAAPYVIHVVGVGHELIADAARLAAALDSAFD
                     IAARLGLKRLAMAPVGLQRGVFSAEESASVLVQVLTARADRAATLPESLVLALTSPSD
                     ASAFRAVVEAPGSPSR"
     gene            4919..5173
                     /locus_tag="VHK28_00990"
     CDS             4919..5173
                     /locus_tag="VHK28_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140834.1"
                     /translation="MTAEGPQQIADALHRRGLDLPARVLLDAHRPLRPLLSQLATFLS
                     PGLRPLAGTRLDAVEAALGDEGSYDDLLAALDETARSRRT"
     gene            5173..6015
                     /locus_tag="VHK28_00995"
     CDS             5173..6015
                     /locus_tag="VHK28_00995"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX2140835.1"
                     /translation="MPDFGEIVGGLVGFLADQFSASSLRLGPIPSVVVFGVVLALLSL
                     VARPTTRWVVRDLGRLAAVGRAMALAAESGAAATFSLGTAGVARAVSAHQRMQTLAAL
                     PILGHVARSAARSGVPLKVTANDPVAVHLAGVAIAEAHRRTATEERAERARAEYLGEG
                     RVVSAAAALSERAAPATAFVMGGLAEEALLLLEGESKDAAWTSYGTAAPSQASSVLLL
                     GHGALIGPELYQAPSDLRSVGHERTSVQAFNRIVGATVVVLLIGSLAGWVTGFDLAGP
                     LAGR"
     gene            6018..6635
                     /locus_tag="VHK28_01000"
     CDS             6018..6635
                     /locus_tag="VHK28_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140836.1"
                     /translation="MRRSLAAATVVVVGLLLLVDFVIINPLLVGAAGAVLELVVLLAA
                     AAAVAGAVALVLRHWTELVERRGDPLGSLVLLTGFTVMLLAGLYPGSDGAADPAVRWL
                     VAALMAPLAAALFSLLFVFLLGALGQGIRLHGRRTAVMLVAAALVVMLLLPLGGPLGD
                     SLAAAASWALAVPIGGVFRGLLIGVGIITALQATRVILAVDGADD"
     gene            6628..7518
                     /locus_tag="VHK28_01005"
     CDS             6628..7518
                     /locus_tag="VHK28_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140837.1"
                     /translation="MTDTGWGGRLRRARRPRPWTVRRPPLLPIVLLLGVAAALLVGRV
                     VDTGTVRLANPDLARVSEWRAALNGLPERPLVLVGMDPDLGTYPEIRAVTRSVLDELA
                     ADGARLAFVSFTPEGRAVAAAEMERLGGSLVLDAGFVAGAEAGLVLAVTDLPGTDASG
                     SVADALREAGGGMAAFDAIVVVGGMDFGPRSWVEQVRTRLPDATILAVAPTFSHPELA
                     PYLRTGQLDGLLATVRDGAAYSALVDGSLPSADDRDRPPDALAMLGGMLLALVFLGRA
                     LLGGVTAAAPADEPVDEGEA"
     gene            7515..8156
                     /locus_tag="VHK28_01010"
     CDS             7515..8156
                     /locus_tag="VHK28_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140838.1"
                     /translation="MSGFFPEGLAGTAATWVAAIVTVAVWAYLVGERRIFRLAQYLLA
                     GLATGYLVLLAVRDVLLPRLVEPLLTEPLATPLLWVALVLVVIMAAARWLPRPVVGIP
                     VAVLVAATAAFALGGAVVGTVLPQVAGAMVRPGSVAGAVDGGIALAISLLVLVAFLHG
                     VPRGRVLSATAGAGRWVLLAGIGGWLGFLVVSRLALVVDRVDFLLFDWIGLAR"
BASE COUNT         1021 a         2918 c         3092 g         1220 t
ORIGIN      
        1 agggggacga ccgcgacgag atcaccgaga tcgcgcacga gctggaccgg cttgcccagg
       61 cgcgcctgaa ctagagactc gaaggagagc gccatgtgcg gcatcgttgg atacgtcggt
      121 ccccgcgacg ctgcccccat cctgctggag ggcctgcgac gcctggagta ccgcggctac
      181 gactccgcgg gcatcgcgct gctgactacc gacggcaacg tcttcgtcga gaagaaggcc
      241 ggcaagctga ccaacctgac cgagcacctc aacggcgggg cgccggccgg ccatcccggc
      301 atcgcccata cccggtgggc cacccacggc cgcccctcgg acctgaacgc gcacccgcac
      361 agcgactgca gcggcaagct ggcgctgatc cacaacggga tcatcgagaa ctacgctgag
      421 atcaaggacc gcctgctggc ggcgggccac aagttcacca gcgagaccga taccgaggtg
      481 ctggcgcacc tcgtggagga gcgctacgac ggtgacctgg tggaggccct gcgcagcgca
      541 ctgtcggacg ttcgcggcgc gtacgccatc ggcgtcatgc atagcgacca ccccgatcgc
      601 atcgtgggtg cccgcatgaa cgtgccgctc atcgtgggcc tgggcaacgg cgagggattc
      661 ctggccagcg acgttcccgc catcctggag cacaccaaga acgtggtcat cctgcacgag
      721 ggtgacatcg ccgatgtctc tccggatggc gtgcggatcc tgcagctgga cggctccgag
      781 gtggagcgcg acgtgaccac catccgctgg aacattgagg cggccgagaa gggcgggttc
      841 aagcacttca ccctcaagga gatctacgag cagccacacg ccatccagga ggcgctgcgc
      901 ggccgcgtgg acgccagcgg gaccatcaag ctccccgagc tggacgccat ccgcgacaag
      961 ctgctggccg ccgagcgcgt gtacctggtc ggctgcggga cggccagcta cgcagccaac
     1021 gtgggtgcct acctgatcca ggagtgggcg cagctgccgg ccttcaccca gatcggcagc
     1081 gagatgcgct acgcgccacc gcccatggac gaccgcacgc tggtgatcag cgtcagccag
     1141 tccggcgaga cggcggatac cctcgccccg cagaagctgg cggcagagcg cggggcgacc
     1201 atcgtggtgg tcaccaacgt cgtcggctcg gccatcaccc gcgaggccga tgcggtctgc
     1261 tacctgcagg ccggcccgga gatcgccgtc gcctcgacca aggccttcgt cactcaggtg
     1321 cttgtgctgc agctgatcgc gctgcacctg gcgcggctgc gcggcaagct ctcggatcac
     1381 cggttgcggg tgtacggcca gggcctgcgc gagcttcccg gcctggcggc cgagacgctc
     1441 aagatccacc cccaggtgaa gaagctggcg cgccgctggc atgacgtgcg cgacgtgatg
     1501 ttcatcggtc gtggcgttgg ctttccgatc gccatggagg gcgccctcaa gctcaaggag
     1561 ctgtcctacg tccacgccga ggggtacgcc gccggcgagc tgaagcacgg cccgatcgcg
     1621 ctgctcgacc acgacacgcc actggtggcc atcgccaccc gcagcgcgac ctacgacaag
     1681 gtggcgagca acatcgccga ggtgcgcgcc cgtgaggccc cggtggtcgc cgtggcgacc
     1741 gagggcgacg aggagatcgc gcgctacgca caggacgtcc tctacgtgcc cgagacgctg
     1801 gagcagttgg cgccggccat cgccatcctg ccgctccagc tgctggcgta cgaggtggcg
     1861 ttggcgcgcg gcacggacgt cgaccagccg cgcaacctgg cgaagtcggt cacggtcgag
     1921 tagcgtccgg gctcgcatgg gccgacacga ggtgggaatc gatctcatcg agatcgagcg
     1981 catcgtcgcc atcctcgcgc gcttcccgga tcgcttccgc gaacggatcc tgaccgaggc
     2041 cgaggcgcgc tattgcggcc gtcgcgccga gcggatcgcc ggccgctggg cggccaagga
     2101 ggccatcagc aaggtcttgg ggctcggggt gcgcggcgtc ggctggcggg agatcgagat
     2161 cctgcccgac tgggccggtg cgccgcaggt ccggctgcac cgtcgcgccg ccgcgcgtgc
     2221 cgcgcgactg ggcctggagg aggtgaccgt ctcgatctcg cacgagcgac agatggcggt
     2281 ggcggtggcc gtcgcccatc gcggggcgga tggctgaccg agtgaagacg gccctgaccc
     2341 agcgctgggt cgccgagcgc ctcccgcccc gtccgccgcg cgctcacaag gggacgttcg
     2401 ggcggctgct ggtggtcgcc ggatccatgg agtacgcggg cgccgccctg ctcgccgggc
     2461 tgggcgcggc acgcacgggg gccgggctcg tctgcctggc cactcccgaa tcggtcggcg
     2521 tacggctgat gggcaacgtc ccggagctcg tcggcctgct gctcgccgag gaggcgccgg
     2581 ggctgatcgc gccgggcggc tggcgtcgat tggcatccga ggcgcgaggc tacgacgccc
     2641 tggtggtggg gccgggcctg gggcgccacc cctcgaccct gcgccgcacc cgtggcttcc
     2701 tctccgagct cgagcggcct gcggtggtcg acgccgatgg gctgaacgcg cttgcggagt
     2761 cggaccgatg gtggcgtccc ttgcgggcgc gcctggtcct caccccgcac ccgggcgagt
     2821 tcgcccgcct gtggggcggc gtcaccgccc agcctctgcc ggacgacgac gacgcacgag
     2881 cggcggctgc gcgcgagggt gccgtgcggt gggggcagac tgtggtgctc aagggtgcac
     2941 gcacggtgat cgccacgccg gagggagagg tgattcgatc ggacgtccag accgccgccc
     3001 tggccaccgc agggagcggc gatgtcctgg ccggcgccat cggcgccttc ctggcgggcg
     3061 gctgcgatcc attggccgcc gccggctgcg gagtgggggt gcacggggcc gccggggtgc
     3121 tggccgagga ccgcatcggc actgccggcg tgctggcccg cgacctggcg gcgctcctgc
     3181 cggcagccat tcgccagctg cgcggccagg acggctcatg agcgcccccg accctcgcag
     3241 cccgcaccgc gcctgggtcg agatcgacca cgccgcgctg cgccacaacc tagaccggct
     3301 gcgcaccatg gccgggctcg agcggcaggt gatcgcggtg gtcaaggcca acgcctacgg
     3361 gcacggggcc atcccggtgg cgcgcacgct gctggacggc ggcgtcgagc gcctggcggt
     3421 ggccaccctg tccgaggcgc aggagctgcg cgtcgcgggc atcgaggcgc cgattctggt
     3481 gctgtggggc ctgggcgcgc ccgaggcacg ggcggcgctg gccgccgacc tggaggcggt
     3541 ggtttacgag cctcgcgggc tggtgctgct ggaggcggcg gcggcggatg ccggccggcg
     3601 cctgtcggtc catctcaagg tggacaccgg gctggggcgg cagggtgccg aacccgagga
     3661 ggccgtggag ctggcggtgc gcatcgcgcg cagccgtcat ctgcagctgt ccggcacctt
     3721 cagccacctg gcggtacccg gcgaggacga cgcctacacc gaggtccagc tcctgcgcct
     3781 tgcacggacg ctggacgcca tgcgctcggc cggcatcgag cccggcctgg tccacgtgtc
     3841 gggtacgggc ggcatcctgg ccggcgccgg caccttcgcg gatgccatcc ggccggggat
     3901 cggcctgtac gggatggcac cctcatggac cgatgccgcc gagcatcggc ttcgcccggt
     3961 gctctcgctc cacgcgctgc cgctgcgcat ctacgacctg ccggggggcg cacccatcgg
     4021 ctacgggctg cgcttccgag ccgagcggcc gaccaggatc gccacgctgg gcatcggcta
     4081 cggcgatggc tggccgcgcg cgcacgccaa caacgggcgg gtcctggtct gcgggcgctc
     4141 cgcgccggtc gtcggcgcca tcagcatgga cggcatcacc gtggatatcg gtgaaatcga
     4201 tgatgtaacc tacgacgacg agttcgtgct catcggtgag caggcgggag ccagcatctc
     4261 ggccgatgag gtcgccgccg agcgtcggac gatcaactac gaggtcacca cggccctccg
     4321 cgagcggctc ccgcgcctgc accgataggg ccgggccgca gttcgggggg cggaacgcga
     4381 gcgagcgctg tatggcagtc cccatcgaga tcgacgtctg gcagggagac gttgcagagc
     4441 tcgaggtcga cgcgatcgtc gtgccggcca acgagagcct gttcatgacc tccccggtag
     4501 cgcgcgccgt gaagctgcgg ggtggagagt cggtggagcg cgatgcggtg gcccagggcc
     4561 cgatcgcggc cgggacggcc atcgtcaccg ctgggggcac gctggccgcg ccgtacgtaa
     4621 tccacgtggt gggggtaggc catgagctga tcgcggacgc ggcgcgtctg gccgcggcgc
     4681 tggactccgc gttcgacatc gccgctcgcc tcgggctgaa gcggttggcc atggcgccgg
     4741 tcggcctgca gcgcggcgtg ttcagcgcgg aggaatcggc ctccgtgctg gtgcaggtgc
     4801 tcactgcgcg ggccgatcgc gcagcgactc tccccgagtc gctggtgctg gccctcacga
     4861 gcccctcgga cgcgtcggcc tttcgtgccg tcgtggaggc cccggggtcg ccgagccggt
     4921 gacggctgag gggccgcagc aaatcgcaga cgccctgcac cgccgggggc tggacctgcc
     4981 cgcgcgcgtc ctgctggacg cgcatcgccc cctgcgtccg ttgctcagcc aactggccac
     5041 cttcctctcc ccggggctcc ggccgctggc gggcacccgg ctcgacgcgg tggaggccgc
     5101 gctgggcgac gaggggtcct acgacgacct gctggccgcg ctggacgaga cggctcggag
     5161 ccgccgcacc tgatgcccga cttcggcgag atcgtcggcg ggctggtcgg cttcctggcc
     5221 gaccagttct cggcgtcctc gctgcgcctg ggaccgatcc cctcggtggt cgtcttcggc
     5281 gtggtgctgg cgctcctctc gctggtggcc cggcccacaa ctcgctgggt agtgcgtgac
     5341 ctgggtcgcc tggcggcggt cggccgcgcc atggcgctgg cggcggagtc cggtgcggca
     5401 gccaccttct cgctgggcac cgccggcgtg gcgcgggcgg tctcagccca ccagcgaatg
     5461 cagaccctgg cggccctgcc catcctgggc cacgtggccc gctcggccgc gcgcagcggc
     5521 gtgccgctga aagtcaccgc caacgatccg gtggccgtgc acctcgccgg cgtggcgatc
     5581 gccgaggcac atcgacgcac cgccaccgag gagcgtgccg aacgggcgcg cgccgagtac
     5641 ctcggcgagg gtcgcgtggt gagcgccgcc gccgccctgt ccgagcgggc ggcaccggcc
     5701 actgccttcg tcatgggcgg cttggccgag gaggcgctgc tcctgctgga gggggagagc
     5761 aaggatgcgg cctggacgag ctacggaaca gctgcgccct cgcaggcctc gtcggtgctg
     5821 ttgctgggcc acggtgcgct catcggtccc gagctgtacc aggcgcccag cgacctgcga
     5881 tcggtcgggc acgagcgtac atccgtgcag gccttcaacc gcatcgtcgg cgcgaccgtg
     5941 gtcgtgctgc tgatcggttc gctggccggc tgggtcaccg gcttcgacct cgctggtccg
     6001 ctggcgggcc gctgacggtg cgccgctcgc tggccgccgc cacggtggtg gtggtcgggc
     6061 tcctcttatt ggtcgacttc gtgatcatca acccgctgct ggtgggagcc gccggcgccg
     6121 tgctggagct cgtcgtgctg ctggcagccg ccgcggcggt ggccggagca gtggccctgg
     6181 tactccggca ctggaccgaa ctggtcgaac gacgcggcga cccactcgga tcgctggtgc
     6241 tgctcaccgg gttcacggtg atgctgctgg cggggctgta ccccggctcg gatggggcgg
     6301 cggacccggc ggtccgctgg ctggtggccg cgctgatggc tccactggcc gctgccctat
     6361 tcagcctcct tttcgtcttc ctgctcgggg cgctgggcca gggaattcgg ctccacggcc
     6421 ggcgcacggc ggtgatgctg gtggccgcgg cgctggtggt catgctgctc ctgccgctcg
     6481 gcgggccgct cggggactcc ctggcggccg cggcctcctg ggcgctggcg gttcccatcg
     6541 ggggcgtgtt ccgcggcctc ctgatcggcg tgggcatcat caccgccctg caggccaccc
     6601 gcgtgatcct ggccgtggac ggcgccgatg actgataccg gctggggggg ccgcctgcga
     6661 cgcgcccggc ggccgcggcc gtggaccgtg cgccggccac cgttgctgcc gatcgtgctg
     6721 ttgctcgggg tcgcggccgc cctgctggtg gggcgcgtcg tcgacaccgg caccgtgcgc
     6781 ctggcgaatc cggacctggc acgggtcagc gagtggcggg cagccctcaa cggcctccct
     6841 gagcggccgc tggtgctggt cggcatggac cccgatctgg gcacctaccc ggagatccgg
     6901 gcggtgaccc gctcggtgct cgacgagctg gcggcggacg gcgcccgcct ggccttcgtc
     6961 agcttcacgc ccgaggggcg ggcggtggcc gcggccgaga tggagcggct gggcggcagc
     7021 ctcgtgctgg atgccggttt cgtggccggc gccgaggccg ggctggtgct ggcagtcacc
     7081 gacctgcccg ggaccgatgc ctccggctcg gtcgccgacg ctctgcgcga ggccggcggt
     7141 gggatggcgg ccttcgacgc gatcgtggtc gtgggcggga tggacttcgg cccgcgctcc
     7201 tgggtcgagc aggttcgcac ccggctgccg gacgccacga tcctggccgt ggctcccacc
     7261 ttctcccatc ccgagctggc gccctacctg cgcaccggcc agctggacgg actgctcgcc
     7321 accgtgcggg acggcgctgc ctacagcgcc ctggttgacg gtagcctgcc gtccgccgac
     7381 gaccgtgacc ggccgcccga cgcgctggcc atgctcggcg gcatgctgct ggcgctcgtg
     7441 ttcctgggcc gtgctctttt gggcggcgtg accgcggccg cgccggccga cgagccggtc
     7501 gacgaggggg aggcatgagc ggcttcttcc cggagggtct ggcggggacg gcggcgacct
     7561 gggtggccgc catcgtcacg gtggccgtct gggcctacct ggtgggcgag cggcgcatct
     7621 tccgcctggc gcagtacctg ctcgccggcc tggcaaccgg gtacctcgtc ctgctggcgg
     7681 tgcgcgacgt tctgctgccg cgcctcgtcg agccgctgct caccgagccg ctggcaacac
     7741 cgctgctgtg ggttgcactg gtcctggtgg tcatcatggc cgccgcgcga tggctgccgc
     7801 ggccggtggt tggcatcccg gtcgccgtgc tggtggccgc caccgcagcc ttcgccctgg
     7861 gcggcgcggt ggtcggcacc gtcctgccgc aggtggccgg cgccatggtc cggccgggga
     7921 gcgtggcagg cgcggtggac ggaggtattg cgctggccat cagcctgctg gtgctggtgg
     7981 cgttcctgca cggcgtgccg cgcgggcgcg tactcagcgc caccgccggc gccggccgct
     8041 gggtgctcct ggccgggatc ggcgggtggc tgggcttcct ggtggtcagc cggctggcgc
     8101 tggtcgtcga ccgggtcgac ttcctgctct tcgactggat cggcctggcc cgatgaccgc
     8161 ccatcccgac gtcgacgacc agcgccagcc ggctgaccac caggaagccc agccacccgc
     8221 cgatcccggc caggagcacc cagcggccgg c
//
LOCUS       DATACA010000048         6063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_488867, whole genome shotgun sequence.
ACCESSION   DATACA010000048 DATACA010000000
VERSION     DATACA010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488867"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VHK28_01015"
     CDS             complement(<1..783)
                     /locus_tag="VHK28_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX2140839.1"
                     /translation="MPTYVRTQLIEHPVGDDGRLSLKVTSADTSVRGVDGEQARVRGT
                     FEISAGSEDEANRIFQEVQLRVRASGDRLAVEEPQDIGSIGSMLSRLFAGRGSVELTV
                     EAELPRHAHLELSGTSADMQVEGMWGDQRYATVSGDLFLNDLGGDVRVNSVSGDVTMR
                     AKLPTAVRAESVSGDVSISAPTIHELRLNSVSGDVEIEGELAPEGDFRADTVSGDVSV
                     GLLGSATFHVRGISTDVDTELDHRLEGRMDRRRVIIGSGRPDF"
     gene            complement(787..1227)
                     /locus_tag="VHK28_01020"
     CDS             complement(787..1227)
                     /locus_tag="VHK28_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEX2140840.1"
                     /translation="MAHDVISTCPVCEGELTISRLHCRSCGTALEGEFQVGRFGRLTR
                     EQLSLLESFLRSRGNLKEMERELGISYPTVRGRVDALVRALDLADSDAPFAEDEEAAG
                     AERIEAIEADAVEEAGAERRAILERLARREISAEDAAAALRELG"
     gene            complement(1228..1380)
                     /locus_tag="VHK28_01025"
     CDS             complement(1228..1380)
                     /locus_tag="VHK28_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140841.1"
                     /translation="MLDYRLHYAILAAEDRLRDSRPHAASQNRGLRRVFRVADSRRQA
                     REAGRR"
     gene            1564..2091
                     /locus_tag="VHK28_01030"
     CDS             1564..2091
                     /locus_tag="VHK28_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2140842.1"
                     /translation="MYQTLLPYSPERDVYRLLQVEPTADEAQIQAACRRLARRFHPDR
                     NVSPRANQEMQVVNAVRALLTDPPSRAAYDRARQRFYQGQRVAAMPSPRTRPVERAPR
                     PTTPPVVALPDLSAIPELAWPAAQTIGRQGRALLRGLRALIARLGPPRCPSCRSLVEA
                     EYRYCADCGTGLLTG"
     gene            complement(2113..4806)
                     /gene="aceE"
                     /locus_tag="VHK28_01035"
     CDS             complement(2113..4806)
                     /gene="aceE"
                     /locus_tag="VHK28_01035"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX2140843.1"
                     /translation="MDRPMHEIFDQFKQQLPDIDPVETQEWLDSLDAVVSQAGPDRAR
                     FIVYKLLKRARQLQVGLPTLTQTRYINTISPEQEPFFPGDEEMELRIRRMIRWNALAM
                     VLRANTKFEGIGGHLSTYASAASLYEVGFNHFFRGSDDGPGDQIYFQGHAAPGIYARA
                     FLEGRLSEEHLDHFRREALTPERGLSSYPHPRLMPEFWQFPTVSMGLGPMAAVYQARF
                     NRYLAAQGIADTSKARVWAFLGDGEMDEPEALGALPIAAREGLDNLVFVVNCNLQRLD
                     GPVRGNGKIIQELEALYRGAGWHVIKVIWAREWDELLARDVDGALVNKMNETLDGDYQ
                     RLSVADGGYIREHFFGPDPRLGRLVEHLSDEQLPKLRRGGHDYRKVYAAYAAAVEHSG
                     APVVILAKTVKGWTLGPGIEGRNVTHQAKKLTLDELKVFRDRLELDIPDDRLKDAPYY
                     HPGPDSPEIQYMLERRKQLGGPVPRRVVVPKPLPAPKDSVFNEFFAGSDRAASTTMAF
                     SRLMRNLVRDPGIGKRIVPIIPDEARTFGMDPLFKEVGIYNPLGQRYTAVDKELLLSY
                     LESETGQLLEEGITEAGAMSSFQAAATAYATWAEPMVPFYTFYSMFGMQRTGDQVWAT
                     GDARGRGFMMGATAGRTTLTGEGLQHDDGQSHVLASAVPNIQAYDPAFAYELAIIIRE
                     GIDRMYGERADDVFYYITLYNENWVQPPKPDGVEEGVIRGLYRFKPASGGSRPAQLLA
                     SGSILQQALRAQEILAEKYDVAADVWSATSFQQLRNDALDVERWNRLHPEAEQRAAYV
                     RRCLDPTQGPVVGVTDYMKAVPDMIGRWVDRPYVVLGTDGFGRSDTREALRDFFEVNA
                     EQIAYAALYGLCLDGKSTVEELQRVIGELGIDPERANPLYA"
     gene            4906..>6063
                     /locus_tag="VHK28_01040"
     CDS             4906..>6063
                     /locus_tag="VHK28_01040"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2140844.1"
                     /translation="MTDSSARPDGQPHRVLILGGGFGGMLTAQELEKRLGRRRDVEIW
                     VVNRDNFMLFTPLLPEVCSGVLEPRHVVVPLRGMVKRPSTWCITAQVEEIDTEAGLVT
                     VLGGDGDLHRLRYDTLVIALGGVTGTFGIPGIDDYAVGMKTLADAFAVRNRMIEMLER
                     AELEDDDAERGAQLTFVVGGGGFSGVETAGEMEEFLRRLMKRYYRKLDRAQIRTVIVE
                     RAGRLLSEMPPEMGEYAQRKLQDRGYEVLLDTPIREVREGAVVVGEGEQERVIATRTV
                     VWAGGVRPAPAVAGSGVEVDRAGRALAQPTMETSRPGVFAIGDCAVIPNVNDPEGRPH
                     APTAQNAVREAAQLARNIMARIDGGPMQPFRYEPLGQLASIGQKTGVGVVFG"
BASE COUNT          915 a         2083 c         2091 g          974 t
ORIGIN      
        1 gaagtccggg cggccagaac cgatgatcac ccgccggcgg tccatgcgac cctccaggcg
       61 atgatccagc tcggtgtcga catcggtcga gatgccgcgc acgtggaagg tggcggagcc
      121 gagcagaccc accgagacgt cgccgctgac ggtgtccgcg cgaaagtccc cctcgggggc
      181 caattcccct tcgatctcga cgtctccgga gaccgagtta agccgcagct catggatagt
      241 cggcgccgag atgctcacgt cccccgatac gctctcggcc cgcaccgccg tgggaagctt
      301 ggcgcgcatg gtgacgtcgc cggatacact gttcacgcgc acgtcgccgc ccaggtcatt
      361 gaggaacagg tcaccggaga cggtggcgta ccgctgatcg ccccacatcc cctcgacctg
      421 catgtcggcc gacgtaccgg acagctccag gtgagcatgg cgcggaagct ccgcctcgac
      481 ggtcagctcc acggaacccc ggccggcgaa caggcgcgac agcatggacc cgatggagcc
      541 aatgtcctgc ggctcctcga ccgccagccg gtctccgctc gcgcgcacgc gcagctgcac
      601 ctcctggaag atccggttgg cctcgtcctc cgacccggcg ctgatctcga acgtgccgcg
      661 cacgcgcgcc tgctcgccgt cgacgccgcg gacgctcgtg tcggccgagg tgaccttcag
      721 cgacaggcgc ccatcgtcgc ccacgggatg ctcgatcagc tgggtgcgaa cgtaggtggg
      781 catcgctcag ccgagctccc gcagggccgc cgccgcatcc tctgcgctga tctctcgcct
      841 ggccaggcgc tcaagtattg cccgccgctc cgcgccggcc tcctcgacag catccgcttc
      901 gatggcttcg atccgctccg cgcccgcggc ctcctcgtcc tcggcgaagg gcgcgtcgga
      961 atcggccagg tccagggcac gcaccagggc gtccacgcgg ccgcggacgg tggggtagga
     1021 gattccgagc tcacgctcca tctccttgag attgccgcgc gatcgcagga agctctccag
     1081 cagcgacagc tgttcgcggg tcaagcggcc gaagcggcca acctggaact cgccctcaag
     1141 cgcggtgccg cagctgcggc agtggaggcg gctgatggtc agctctccct cgcagaccgg
     1201 gcaggtggag atgacgtcat gggccatcta gcggcgcccc gcctcgcggg cctggcgacg
     1261 ggaatcggcg acgcgaaaga cgcgacgcag gccacggttc tggctggccg cgtgcggcct
     1321 gctgtcacga aggcggtcct cggccgccag gatcgcgtag tgaagtcgat agtccaacat
     1381 tggtagccgc tccttcgggt ttagtagttg gtgttgctat ttgtgtcctg gtgagtgcac
     1441 tgtaagggga tagtatcggt ttgtcaaccc ctgctgccaa agtagcaagc cgacctacca
     1501 aaatgaggct gtacaactgg tgctagcacg caccgaatcg ctccgctacc atgccctcga
     1561 cccgtgtacc agaccctgct gccgtactca cccgagcgcg acgtctaccg ccttttgcag
     1621 gtagagccga ccgccgacga ggcccagatc caggccgcct gccgccgcct ggcgcgccgg
     1681 tttcacccgg atcgcaacgt ctcgccgcgt gccaaccagg agatgcaggt ggtcaacgcc
     1741 gtgcgcgcgc tcctgaccga tcctccgtcg cgggccgcct acgatcgcgc gcgtcagcgg
     1801 ttctaccagg gacagcgcgt cgcagccatg ccctcgccgc gaacgcgtcc ggttgaacgg
     1861 gcgccccgcc ccacgacgcc cccggtggtg gccctgcccg acctatcggc cattccggag
     1921 ctggcctggc ccgcggcgca gaccatcggc cgccaggggc gcgcgctgct gcgcgggctg
     1981 cgggcgctca tcgccaggct cggcccgccg cgctgcccgt cctgccgttc gctggtggag
     2041 gcggagtacc gctactgcgc cgactgcgga accgggctct tgaccggctg agcggctcag
     2101 ggaaaaggcc ggtcaggcgt agagcgggtt ggcgcgttcc ggatcgatcc cgagctcacc
     2161 gataactcgc tgcagctcct cgactgtgga cttgccgtcc aggcagagtc cgtacagcgc
     2221 ggcgtaggcg atctgctcgg cgttgacctc gaagaagtcg cgcagcgcct cgcgggtatc
     2281 cgagcggccg aagccatcgg tccccagcac gacgtacggt cggtccaccc agcggccgat
     2341 catgtccggc acggccttca tgtagtcggt gacgccgacc accgggccct gggtgggatc
     2401 caggcagcgg cgcacgtagg ccgcgcgctg ctcggcctcg gggtggaggc ggttccagcg
     2461 ctccacgtcc agcgcatcgt tgcggagctg ctggaagctg gtcgcgctcc acacgtcggc
     2521 ggccacgtcg tacttctcgg ccaggatctc ttgcgcccgc agggcctgct gcaggatcga
     2581 gccggaggcc agcagctgcg ccggccgtga gccgccggag gcgggcttga acctgtacag
     2641 gccgcggatc acgccctcct cgaccccgtc cggcttgggc ggctgcaccc agttctcgtt
     2701 gtagagggtg atgtagtaga agacgtcgtc ggcgcgctcg ccgtacatgc gatcgatccc
     2761 ctcgcggatg atgatcgcca actcgtaggc gaaggccggg tcgtaggcct gtatgttcgg
     2821 gacggcgctg gcgagcacgt gcgactgccc gtcgtcgtgc tgcaggccct caccggtaag
     2881 cgtcgtgcgg ccggcggtgg cgcccatcat gaatccgcgg ccgcgggcgt cgccagtggc
     2941 ccacacctgg tcgccggtgc gctgcattcc gaacatcgag tagaaggtgt agaagggcac
     3001 catcggctca gcccaggtgg cgtacgccgt ggcggccgcc tggaagctgg acatggcacc
     3061 cgcctcggtg atgccctctt ccagcagctg cccggtctcc gactcgaggt agctgagcag
     3121 tagctctttg tcgaccgcgg tgtagcgctg gcccaagggg ttgtagatcc cgacctcctt
     3181 gaagagcggg tccatgccga aggtccgggc ctcgtccgga atgatgggca cgatccgctt
     3241 gccgatgccc gggtcgcgca ccaggttccg catcagccgg ctgaaggcca tggtcgtgct
     3301 cgccgcgcgg tcggaaccgg cgaagaattc gttgaacacg ctgtccttgg gggccggcag
     3361 tggcttcggc accacgaccc gccgcggcac cgggcctccc agctgcttgc gacgctcgag
     3421 catgtactgg atctcgggcg aatcggggcc gggatggtag tacggcgcgt ccttgagcct
     3481 gtcgtccggg atgtcgagct ccagccgatc acggaagacc ttcagctcgt ccagggtgag
     3541 cttcttggcc tggtgggtca cgttgcggcc ctcgatgccc gggcccagcg tccagccctt
     3601 gacggtcttg gccaggataa cgaccggggc gccgctgtgc tccacggcgg cggcgtaggc
     3661 ggcgtacacc ttgcggtagt cgtgcccgcc gcggcgaagc ttggggagct gctcgtccga
     3721 caggtgctcc accaggcgac ccagccgtgg gtcgggaccg aagaagtgct cgcggatgta
     3781 cccgccgtcg gccaccgaca gccgctggta gtcgccgtcg agggtctcgt tcatcttgtt
     3841 gaccagcgcg ccgtcgacgt ctcgggccag cagctcgtcc cactcgcggg cccagatgac
     3901 cttgatcacg tgccagccgg caccgcggta cagcgcctcc agctcctgga tgatcttgcc
     3961 gttgccgcga accgggccgt ccagccgctg gaggttgcag ttgaccacga agaccaggtt
     4021 gtcgaggccc tcgcgagcgg cgatcggcag cgcgccgagc gcctcgggct cgtccatctc
     4081 gccatcgccc aggaaggccc acacccgggc cttgctggtg tcggcgatgc cctgcgccgc
     4141 caggtagcgg ttgaaccgcg cctggtagac ggcggccatg ggtcccagcc ccatgctgac
     4201 ggtggggaac tgccagaact cgggcatcag ccgcgggtgc gggtaactgg acaggccgcg
     4261 ctccggcgtc agcgcctcgc ggcggaagtg gtctagatgc tcctcactca agcgtccctc
     4321 gaggaacgcg cgcgcgtaga tgcccggtgc cgcgtgaccc tggaagtaga tctggtcacc
     4381 ggggccgtcg tccgagccgc ggaagaagtg gttgaagccg acctcgtaca ggctggcggc
     4441 gctggcgtag gtggacaggt ggccgccgat gccctcgaat ttggtgttgg cgcgcagcac
     4501 catggccagc gcgttccagc ggatcatgcg ccggatgcgc agctccatct cctcgtcgcc
     4561 ggggaagaag ggctcctgct cggggctgat ggtgttgatg tagcgcgtct gggtcagcgt
     4621 cggcagcccg acctgcagct ggcgcgcccg cttgagcagc ttgtagacga tgaaccgggc
     4681 tcgatcgggt ccggcctggc tcaccaccgc atccagcgag tcaagccact cctgggtctc
     4741 caccgggtcg atgtccggca gctgctgctt gaactggtcg aagatctcgt gcatcggtcg
     4801 gtccatgtgc cacctatggt acagaccgat gcggaggtgg gcatttcgcc actccgagcc
     4861 agccaatcga ggtcacggca ctcgacttgg tggcagtatc tgtgggtgac cgacagcagc
     4921 gcgcggcccg acgggcagcc acatcgggtc ctgatcctgg gcggcggctt cggcggcatg
     4981 ctgaccgccc aggagctgga gaagcgcctg ggccggcgcc gcgacgtcga gatctgggtg
     5041 gtgaaccgcg acaacttcat gctgttcacc ccgctcctgc cggaggtctg ctccggcgtg
     5101 ctggagccgc ggcacgtggt cgtcccgctg cgcggcatgg tcaagcgccc cagcacgtgg
     5161 tgcatcaccg cgcaggtgga ggagatcgac accgaggccg gcctggtcac cgtgctgggt
     5221 ggcgatggcg acctccaccg gctgcgctac gacacgctgg tcatcgccct gggcggcgtg
     5281 accggcacct tcggcatccc cggcatcgac gactacgcgg tcggcatgaa gacgttggcc
     5341 gatgctttcg ccgtgcgcaa ccgcatgatc gagatgctgg agcgcgccga actggaggac
     5401 gatgacgcgg agcgtggggc gcagctcacc ttcgtggtcg gcggcggcgg gttcagcggg
     5461 gtcgagaccg cgggcgagat ggaggagttc ctgcgccgcc tgatgaagcg ctactaccgc
     5521 aagctcgacc gcgcccagat ccgcaccgtc atcgtcgagc gtgccggccg gctgctgtcc
     5581 gagatgccgc cggagatggg cgagtacgcg cagcgaaagc tccaggaccg cggctacgag
     5641 gtcctgctcg acacccccat ccgcgaggtg cgcgagggag cggtggtggt cggcgagggg
     5701 gagcaggagc gcgtcatcgc cacccgcacc gtggtctggg ccggcggggt tcgaccggcc
     5761 ccggcggtcg ccggctctgg tgtggaggtc gaccgtgccg gtcgcgcact ggcccagccg
     5821 acgatggaga ccagccgccc cggcgtcttc gccatcggcg actgcgcggt aatccccaac
     5881 gtcaacgatc cggaaggccg gccgcacgca ccgacagccc aaaacgccgt gcgcgaggcg
     5941 gcacagctgg cgcgcaacat catggcccgt atcgacggtg gaccgatgca gccattccgc
     6001 tacgagccgc tcgggcagct ggcttccatc ggtcagaaga caggggtggg cgtcgtcttc
     6061 ggc
//
LOCUS       DATACA010000049         6201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2095608, whole genome shotgun sequence.
ACCESSION   DATACA010000049 DATACA010000000
VERSION     DATACA010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095608"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1438
                     /locus_tag="VHK28_01045"
     CDS             95..1438
                     /locus_tag="VHK28_01045"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140845.1"
                     /translation="MAPEPALAARAAALDADWLSRRRWFRSKRRPLGGVEVVDAASLD
                     GPGSWLLVLRARFRDGGEERYLVPAVEDGGRLREPEDGDGAWLAIARRAAAGPPEAAG
                     QGSTFPFESTPAHGELLDAGGDPATTLASLGERRFTGEQSNTSVRLGDVLVLKIYRLL
                     EPGTNPELEVNAFLTRLGFRYAPALGGVVSYHPANGEPAAAAMLQALVPSRGDGWDWV
                     LRSLASPPDGPLRAMAGASQVGGVTAELHAALAARPEEEGFPARPATGEERQAWRRGA
                     EERLAAALGALDGGDAERLERLAPRIRERFAAIEDAEGARLSRIHGDYHLGQLLRTDE
                     GFMVVDFEGEPARPLAERRHPASPLRDVAGMLRSLDYAAQMGERQADRSFDAEGWLSE
                     ARGAFLTAYGFMGPAEEALLAAFELEKACYEVAYEANNRPAWTWLPLAAVEQLAR"
     gene            1590..2627
                     /locus_tag="VHK28_01050"
     CDS             1590..2627
                     /locus_tag="VHK28_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2140846.1"
                     /translation="MGSGSDPSGRTRDDRLRAHSVVVSLVLVAVFVPMLDDMARRSLR
                     VGGVELPSQQSVAGAADSATPGASAGAPVLPTATPDQVPELVLAVGDLTNCPEMSSGV
                     PELVASLPGPLLAVGDIVDPGGSYQEYRDCYEPQWGALRDRIWPVPGNHDYNVPGAGP
                     YFAYFGLDAGTPGAGWYSRDLGWWHVVALNSICPAVGGCDQDSQQVQWLIEDLAAHPA
                     RCTLAFFHHPRFSSGDGAAPPRTETIWKVLYDAGADVVLNGHDHHYDRFAPLAPDGSI
                     DERRGIRQFTVGTGGASRSRGTGQAPQSEFLTREHHGVLQLALHRDRYEWQFISAADG
                     AVIDSGSDTCH"
     gene            2692..4827
                     /gene="glgX"
                     /locus_tag="VHK28_01055"
     CDS             2692..4827
                     /gene="glgX"
                     /locus_tag="VHK28_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX2140847.1"
                     /translation="MPSSVIAPTRVWRGNPYPLGASWDGGGVNFALFSENAHAVDLCL
                     FDADGAETRIRLNEQTDQVWHGYLPDVRPGQRYGYRVHGPYEPGQGHRFNPHKLLIDP
                     YAKRVDGSVNWSDRLFGYRVGGDDDDQPDDRDSAADVPKCVVVDPTFMWTDDRLLKTP
                     WEETIIYEVHVKGFTQQHPEVPSELRGTYAGLASPAAIDYLTQLGVTAVELLPVHHHV
                     NDRHLVERGLTNYWGYNTIGFFAPDSRYAATEDPVTEFKSAVKRLHDAGMEIILDVVY
                     NHTGEGNHLGPTLSFRGIDNAAYYRLSPEDPRFYMDYTGTGNTLNGVHPRALQLIMDS
                     LRYWITEMHVDGFRFDLASALAREMYDVDKLGSFFDIIHQDPIISQAKLIAEPWDVGP
                     GGYQVGNFPVGWAEWNGKYRDSVRRFWRGESGQTAELAYRLTGSSDLYAQGGRLPHAS
                     INFVTAHDGFTLNDLVSYESKHNEANGEDNRDGESNNESMNFGVEGPTQDPAIVAIRE
                     QQKRSFLATLFLSQGVPMLLGGDEIGRTQRGNNNAYCQDNEISWYDWRLTDASRALLE
                     FTRGMIAFFRAHPVLRRRRFFQGRRIRGSSVKDLTWYGPGGVEVDDEQWHAEAVHTVG
                     LRLAGDAIEERGPRGETIEDDTLLMIFSAAEEPTEFRLPTDSGRPWQLVLDTSQPDPP
                     SLDGGPHHEGGSTYSVAARSVVAFLMSPD"
     gene            4886..>6201
                     /locus_tag="VHK28_01060"
     CDS             4886..>6201
                     /locus_tag="VHK28_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2140848.1"
                     /translation="MTEPRATYRLQLHAAFDLDAAAGIVPYLARLGISHLYVSPILQA
                     EAGSTHGYDVVDHSRINIELGGEAAFDRLVAALRGHGIGLVVDVVPNHMAIGPANRWW
                     WDVLTHGPASRYARYFDVDWDPPESRLRNVILLPVLGDHYGRILEAGGLKVERRGAEL
                     VVRHDEQRWPLDPRSLAPLVAAAARRSGSAELAHVARALDGLPPTGAEVPADVERRSA
                     DGAKLSERLEVLVADPALSGALDAELDEVNADHDRLDALLEEQSYRLAFWRASSRDLG
                     YRRFFDINSLAALRVEIPEVFEATHRLILDRVRAGEISGLRIDHPDGLRDPAGYFARL
                     REEAPDAWIVAEKILEADEPLRDWPIDGTTGYRFANLATGLLVDPAGAKPMTDAFAAV
                     TGAPTGWTEVAREARLLTLSASLGSDVNRLTELWLTICEAHRRYRD"
BASE COUNT          889 a         2228 c         2208 g          876 t
ORIGIN      
        1 ggcagggtgc gcttcccgcc catcggcgag cttccctacc tgctcacctt cggcccgcac
       61 gggtactact ggttcgagct gaccgatggc gccgatggcg cctgagccgg cgctggccgc
      121 gcgcgccgcg gcactggatg ccgactggct ctcccgtcgt cgatggttcc gcagcaagcg
      181 ccgtccgctc ggcggcgtgg aggttgtgga cgcggcgtcg ctcgatggcc ccggcagctg
      241 gctgctggtc ctgcgcgccc gcttccgcga cggcggcgag gagcggtatt tggtgccggc
      301 cgtcgaggac ggcggtcgcc tgcgtgagcc ggaggacggc gatggtgcct ggctggcgat
      361 cgcacggcgc gccgcggccg ggccgcccga agctgccggc cagggcagca cgttcccctt
      421 cgagtccacg cctgcccacg gtgagctgct cgacgccggc ggcgatccag ccaccaccct
      481 agcctcgctc ggcgaacggc gattcaccgg cgagcagtcg aacacctcgg tccggctggg
      541 cgacgtgctg gtgctgaaga tctaccggct gctggagcca ggcaccaacc ccgagctgga
      601 ggtgaacgcc ttcttgaccc ggctcgggtt ccggtacgcc ccggcactgg gcggcgtggt
      661 cagctaccac cctgccaacg gcgagccggc cgccgccgcc atgctccagg ccctggtccc
      721 gtcgcgcggc gacgggtggg actgggtgct gcgctcgctg gcctcgccgc cggacgggcc
      781 gctgcgggcc atggccgggg cttcccaggt cggcggcgtg acggccgagc tgcacgcggc
      841 gctggcggcg cggccggagg aggagggatt cccggcgcgg ccggcgaccg gcgaggaacg
      901 ccaggcgtgg cggcgcggcg cggaggagcg gctggcggcc gcgctcggag cgctggacgg
      961 cggcgatgcc gagcgcctgg agcggctggc gccccgaatc cgggagcggt ttgccgccat
     1021 cgaggacgcc gagggggcgc ggctgtcccg gatccacggc gactaccacc tgggccagct
     1081 gctgcgcacc gacgagggct tcatggtcgt cgacttcgag ggcgagccgg cgcgcccgct
     1141 ggccgagcgc cgtcacccgg catctcccct gcgagacgtg gccggcatgc tgcgctcgct
     1201 cgactacgca gcgcaaatgg gcgagcggca ggcggaccgc tccttcgacg ccgagggctg
     1261 gctgtccgag gcgcgcggcg cgttcctcac ggcctacggg ttcatgggtc cggccgaaga
     1321 ggcgctgctg gccgccttcg agctggagaa ggcctgctac gaggtggcct acgaggccaa
     1381 caaccggccg gcctggacgt ggctgccgct ggccgccgtg gagcagctgg cccgctgacc
     1441 gcgcacctcc ctccgcggcg cctgattccg gggactcttg aggcggccgc cgctatcgcc
     1501 gacaatggcc ggcctccgtt cgggccggcg gcggtggcat cggccgtgcg ggaacggcca
     1561 gcggtgaggt agtgcaggga ggtgcgatcg tgggcagcgg gtccgacccg tcggggcgga
     1621 ccagggacga ccggcttcgg gcgcactcgg tggtggtgag cctggtgctg gtcgccgtct
     1681 tcgtccccat gctggacgac atggcccggc ggtccctgcg ggtcggaggc gtcgagctgc
     1741 cctcccagca gagcgtggcc ggggccgcgg acagcgcaac ccccggcgcg agcgcggggg
     1801 cgccagtcct cccgaccgcc acccccgacc aggtaccgga gctggtgctg gccgtgggcg
     1861 acctcacgaa ctgcccggag atgtcgagcg gcgtgccgga gctcgtcgcc tcgctgcccg
     1921 gaccgctgct ggcggtgggt gacatcgtcg accccggggg ctcgtaccag gagtatcgcg
     1981 actgctacga gccgcaatgg ggcgcgctgc gggaccggat ctggccggtt cccggcaacc
     2041 acgactacaa cgtgcccggc gcgggcccct acttcgcgta cttcggcctg gacgccggca
     2101 cgccgggcgc cggctggtac agccgggatc tcggctggtg gcacgtcgtc gccctgaaca
     2161 gcatctgtcc ggcggtgggg ggatgcgacc aggactcaca gcaggtgcag tggctgatcg
     2221 aggacctggc cgcgcacccg gcgcgctgca cgctggcctt cttccaccac ccacgcttca
     2281 gctccggcga cggcgccgcc ccaccccgca cggagacgat ctggaaggtg ctctacgatg
     2341 cgggcgccga cgtcgtgctc aacggccatg accaccacta cgaccgtttc gccccgttgg
     2401 ccccggacgg gtccatcgac gagcggcggg gcatccggca gttcaccgtc ggcaccggcg
     2461 gcgccagccg ttcgcgcggc accgggcagg caccccagag cgaattcctc acccgcgagc
     2521 atcacggcgt gctgcagctt gccctgcacc gcgaccgcta cgagtggcag ttcatctccg
     2581 ccgccgacgg ggcggtgatc gactcgggct cggacacctg ccattgaccg accgcagacc
     2641 gctggcgcca cagcgccacc gaggccgtgg tcgttccgta tccttggcgg gatgccctcc
     2701 tcggtgatcg cgcccacgcg ggtgtggcgc ggcaacccgt accccctggg cgcctcctgg
     2761 gacggaggcg gcgtgaactt cgcgctgttc agcgagaacg cccacgcggt ggacctgtgc
     2821 ctgttcgacg ccgatggcgc cgagacccgc atccggctca acgagcagac cgaccaggta
     2881 tggcacggct acctccccga cgtgcggccc gggcagcgct acggctaccg cgtccacggg
     2941 ccgtacgagc cgggacaggg gcatcggttc aacccgcaca agctgctgat cgatccctac
     3001 gccaagcggg tggacggatc ggtcaactgg agcgatcggc tcttcggcta ccgggtggga
     3061 ggggatgacg acgaccagcc cgatgatcgc gactcggctg ccgacgtgcc caagtgcgtg
     3121 gtcgtcgacc ccaccttcat gtggaccgat gaccgtttgc tgaagacgcc ctgggaggag
     3181 acgatcatct acgaggtcca cgtcaagggc ttcacccagc agcatccgga ggtgccttcc
     3241 gagctgcgcg gcacctacgc cgggctggcc tcgcctgcgg cgatcgacta cctgacccag
     3301 ctgggagtga ccgctgtaga gctgctgccc gtgcaccacc acgtcaatga ccggcacctg
     3361 gtcgagcgcg ggctgaccaa ctactggggc tacaacacca tcggcttctt cgcgcccgac
     3421 tcgcgctacg cggccaccga ggacccggtc accgagttca agagcgcggt caagcggctg
     3481 cacgacgccg gcatggagat catcctggac gtggtctaca accacaccgg cgagggcaac
     3541 cacctggggc cgaccctctc cttccgaggc atcgacaacg ccgcctacta ccgcctctcg
     3601 ccggaggacc cgcgcttcta catggactac accgggaccg ggaacacgct caacggcgtc
     3661 cacccgcgcg cgctgcagct gatcatggac agcctgcgct actggatcac cgagatgcac
     3721 gtggacggct tccgcttcga cctcgcctcg gcgctggcgc gcgagatgta cgacgtcgac
     3781 aagctgggca gcttcttcga catcatccac caggacccca tcatcagcca ggcgaagctg
     3841 atcgccgagc cgtgggacgt cggcccgggc ggctaccagg tcggcaactt cccggtcggc
     3901 tgggccgagt ggaacggcaa gtaccgcgat tcggtgcggc gcttctggcg cggcgagtcc
     3961 ggccagaccg cggagctggc ctatcggctg accggctcat cggacctgta cgcccaggga
     4021 ggtcggttgc cgcatgccag catcaacttc gtgaccgctc acgacggctt caccctcaac
     4081 gacctggtga gctacgagtc gaagcacaac gaggccaacg gcgaggacaa ccgcgacggc
     4141 gagtcgaaca acgagagcat gaacttcggc gtggaggggc ccacccagga cccggccatc
     4201 gtggccatcc gcgagcagca gaagcgcagc ttcctggcca cgctcttcct cagccagggc
     4261 gtgcccatgc tgctgggggg cgacgagatc ggtcgcaccc agcgcggcaa caacaacgcc
     4321 tactgccagg acaacgagat cagctggtac gactggcgcc tgaccgatgc ctcccgcgcg
     4381 ctcctggagt tcacgcgcgg catgatcgcc ttcttccggg cccaccccgt gctgcgccgc
     4441 cggcgattct tccagggtcg tcgcatccgc gggtcatcgg tcaaggacct gacctggtac
     4501 gggcccggcg gggtggaggt cgacgacgag cagtggcatg cggaggcggt gcatacggtc
     4561 gggctgcggc tggccgggga tgccatcgag gagcgcgggc cccgcggcga gacgatcgag
     4621 gacgacaccc tgctgatgat cttcagcgcg gccgaggagc cgaccgagtt ccgcctgccg
     4681 accgactccg ggcggccctg gcagctggtg ctcgacacca gtcagcccga cccgccgagc
     4741 ctggacggcg gaccgcacca cgagggcggc tccacctact cggtcgccgc ccgctcggtg
     4801 gtcgccttcc tgatgtcgcc ggactgagcc gctcatccgc gatcacgcgg gctttggtat
     4861 gccacgtgcc tcatccgggc gcgtcatgac cgagccgcgc gccacctacc gcctccagct
     4921 gcacgccgcg ttcgacctgg acgcggcggc cggcatcgtc ccgtatctcg cccgtctcgg
     4981 catcagccac ctgtacgtgt ccccgatcct gcaagctgag gcgggcagca cgcacggcta
     5041 cgacgtggtg gaccactccc gcatcaacat cgagctcggc ggcgaggcgg ccttcgaccg
     5101 cctggtcgcg gcgctgcggg gccatggcat agggctggtg gtcgacgtgg tgcccaacca
     5161 catggccatc ggtcccgcca accgctggtg gtgggacgtc ctgacccacg gccccgcctc
     5221 acggtacgcg cgctacttcg acgtggactg ggacccgccg gagagccggc tgcggaacgt
     5281 gatcctgctg cccgtcctgg gcgaccacta cggccggatc ctggaggcgg gcgggttgaa
     5341 ggtggagcgg cgcggcgcgg agctggtggt gcgacacgac gagcagcgct ggccgctcga
     5401 cccgcgcagc ctggcgccgc tggtggccgc cgccgcacgg cgcagtggct cggccgagct
     5461 ggcgcacgtc gctcgcgccc tggacggcct gccgccgacc ggcgccgagg tgccagccga
     5521 cgttgagcgc cggagcgccg acggcgcgaa gctgtcggag cggctggagg tcctggtggc
     5581 ggacccggcg ctgagcggtg cgctggatgc cgagctggac gaggtgaacg ccgaccacga
     5641 tcgcctcgac gccctgctcg aggagcagag ctaccggctc gccttctggc gagcgtcgag
     5701 ccgcgacctc gggtaccggc gcttcttcga catcaactcg ctggccgctc tgcgggtgga
     5761 gatccccgag gtgttcgagg ccacgcaccg cctgatcctg gaccgtgtgc gggcgggcga
     5821 gatcagtggg ctgcgcatcg accatcccga cggcctgcgc gacccggccg gctacttcgc
     5881 ccggctccgg gaggaggcgc cggacgcctg gatcgtggct gagaagatcc tggaggcgga
     5941 cgagcccctg cgcgactggc cgatcgacgg caccaccggc tatcggttcg ccaacctggc
     6001 gaccggcctg ctggtggacc ccgccggtgc caagccgatg accgatgcct tcgccgccgt
     6061 caccggagct cccactggct ggaccgaagt agcgcgcgag gcacggctgc tgacgctgtc
     6121 cgcgtcgctg ggcagcgacg tcaaccgcct gaccgagctg tggctcacca tctgcgaggc
     6181 gcaccgccgg taccgcgact t
//
LOCUS       DATACA010000050         8573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1453055, whole genome shotgun sequence.
ACCESSION   DATACA010000050 DATACA010000000
VERSION     DATACA010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453055"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VHK28_01065"
     CDS             complement(<1..242)
                     /locus_tag="VHK28_01065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610481.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEX2140849.1"
                     /translation="MTTNAVISGWGMYAPRRVMTNEELSTLVDTSDEWIVSRTGIHER
                     RIAADDETTTTLSVHASRDALAVAGVDPRDVDLVIVG"
     gene            complement(258..734)
                     /locus_tag="VHK28_01070"
     CDS             complement(258..734)
                     /locus_tag="VHK28_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2140850.1"
                     /translation="MTERAVDPVLAAVDLLTPAFEASGLDELEVEAGDLVIRLARPRA
                     AVLAAPAATPATMPAPPPVESPAPASPYGQPGPGLRFVTAPLTGVWYAAPSPGARPYL
                     SDGSEVGVGSVIGLIEAMKLFNEIKSDVVGRVTRILVESGTLVKRQQPLIEVDPSG"
     gene            complement(731..2041)
                     /gene="fabF"
                     /locus_tag="VHK28_01075"
     CDS             complement(731..2041)
                     /gene="fabF"
                     /locus_tag="VHK28_01075"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX2140851.1"
                     /translation="MTDAYVQPTAYAQPSSAERGERRVVITGMGAVTPLGNDVPSFWD
                     GLKSARSGIGPITSFDPARVPSRVAGEIRGFDADAVMPRKEVRRNDRYVHYAWSAATQ
                     ALSDAGIAYPIADEHEAMRTGAIIGTGIGGINTMIRDIIEAHELGPDRVGPFLVTAMI
                     PDMAAGFIAIYANARGPNYATVSACASSNHAIGSALSLIRRGDADVMLAGGAEAGIGE
                     IPVAAFAAMRALSTRCNDEPERASRPFDALRDGFVIADGAGLVVLEELEHARARGATI
                     HAEVVGYGATDDASHITLPAPGGRGAVGSMSLALADAGLTTGDVDYINAHGTSTPPND
                     RSETAAIKTLFGERAAAIPVSSTKSMTGHLLGAAGGIEAIACVLAITNGIVPPTINYE
                     NPDPDCDLDYVPNQARQVEVRTAMSNSFGFGGHNATLVFRRYAA"
     gene            complement(2034..2486)
                     /gene="fabZ"
                     /locus_tag="VHK28_01080"
     CDS             complement(2034..2486)
                     /gene="fabZ"
                     /locus_tag="VHK28_01080"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX2140852.1"
                     /translation="MEAAAIMEILPHRYPFLLVDRVLELEPGQRAVAIKNVTANEPQF
                     TGHFPTRPIMPGVLMVEALAQTAGLAVSTLDAYRGKLGLFAGIDECRFRRMVQPGDTL
                     RLEVTVEKLRGMFGRVRGVASVDGEVAVEATLSIIFPRDQSLGGGTDD"
     gene            complement(2545..3909)
                     /gene="accC"
                     /locus_tag="VHK28_01085"
     CDS             complement(2545..3909)
                     /gene="accC"
                     /locus_tag="VHK28_01085"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2140853.1"
                     /translation="MFGKILIANRGEIAVRILRACRDLGVPAVVAYSEADRDSLAVHM
                     ADEAVCIGPAEARKSYLNQPAVVSAALITGCDAIHPGYGFLSEDAGFAEVVAAHDLAF
                     IGPRAEVLDRFGSKYAVRRMLAANGLPTVPGSRGILTDLADALSQAQQSGYPVLIKPS
                     GGGGGRGMRLVRSPRELETSLPLARSEAQAAFGDDSVYFEKWIEESRHVEVQVLIDQH
                     GNGIHLGERDCSVQRRHQKIIEEAPSPAMDPAARERLRDLAIRAVIAAGYESAGTLEF
                     LLDADGSFYFIEINCRIQVEHPVTEMLTGVDLIAEQIRLAAGERLSLRQEQVSLRGHA
                     IEFRVTAEDPNDNFAPQTGTIEELELPSGPGVRVDTHLYRGYEVPPFYDSLLAKLIVW
                     GETREMAMARSRRALAEFRIEGIKTNLAFHRGIVDNAAFLQAEVSTNLLDRVGPAAFV
                     AGGA"
     gene            complement(3911..4888)
                     /gene="fabD"
                     /locus_tag="VHK28_01090"
     CDS             complement(3911..4888)
                     /gene="fabD"
                     /locus_tag="VHK28_01090"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2140854.1"
                     /translation="MSLAIVFSPQGSQSVGMGRELATTWPSAAAVFAEADAALGWPVS
                     TLCWDGPAERLNDTRQTQPCLVATSLACLRALEEASAGVGRALSPAFMAGHSVGEYAA
                     LAAAGVLSLADALRLVALRGALMADAGADGGMAAVIGLDREAVAQAVERAASEAGTGH
                     DPVLANDNAPGQVVISGSRPALAAAQPHLSAAGARRIIPLNVSGPFHSPFMADAGRRL
                     AEAFGDVAWSEADPPLVANVNAQPVREPNEIRELLARQVSAPVEWVRSVQRLVADGVD
                     TIVECGPGQALTGMVKRIAPELRALNVMDSTSLAATLRELTSTTAEASV"
     gene            complement(4885..5373)
                     /locus_tag="VHK28_01095"
     CDS             complement(4885..5373)
                     /locus_tag="VHK28_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2140855.1"
                     /translation="MTDQDERLSEVRRAAEDLVPRLTQRLERHGLGEIEVRLGTLRVK
                     VSAASSPGPTAAGSHATSEPAPRLSVEHAPHGENPGHGPAVTSHLAPVTSPAVGFFGY
                     SDGLGPGLAVQKGDELGHVEMLGVRHDVRAPRGGTVRNLVTEAGEPVEYGQLLIELEP
                     VE"
     gene            complement(5370..6383)
                     /locus_tag="VHK28_01100"
     CDS             complement(5370..6383)
                     /locus_tag="VHK28_01100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX2140856.1"
                     /translation="MSDLLPPQVRAFGDTPPSQEQVDEAWRRVQMARHAQRPRTLDLV
                     PRIFEDFVELHGDRSYRDDPAIVGGPALLDGRPVMVIGHQKGSDTESNITRNFGSPHP
                     EGFRKAQRLMRLAEKLGLPVVTLLDTAGAFPGPAAEERGQAEAIASSIKQMTALQVPV
                     VVVIVGEGGSGGALAIGVGDEVLALENAVYSVISPEGCASILWRAPDKAPQAAAAMRM
                     TAADQLQLGVIDRIIEEPGEGAHTDHAATARAIKAALLAALGRVSRLEPEALLATRYA
                     RLRGIGLFREAEAPDGRALEEQTLRRRLGRFLRLPGVPRRPRWSEIWPSGDDGGGGGE
                     GGA"
     gene            complement(6380..7408)
                     /gene="accD"
                     /locus_tag="VHK28_01105"
     CDS             complement(6380..7408)
                     /gene="accD"
                     /locus_tag="VHK28_01105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX2140857.1"
                     /translation="MRIKFPLRRRDGPADAWTKCPSCDTQVFNRQLERNLRVCPTCGH
                     HFRMSIGERIDLLLDADSFAERDAGLESADPLGFHDQKPYPDRIEASRVKTGLREAAV
                     WGTGRIDGRPVVIGLFDFRFMGGSMGSVVGEKIARCFEAALTERVPAIMVSASGGARM
                     QEGTLSLLQLAKTTAPLTRMDLAGVPYISVLTDPTTGGVLASFASLGDVVLAEPRALI
                     GFAGARVASGTVGEELPDGFQTAEFLLEHGFVDLVVPRPELRATLSRLLRLLPVAAVD
                     ERLAEDGPRGWGPISVLSGLAERVGGAVSETIGLDGVQQDGDGAPPRERSGAERTGAG
                     PNNGTGEA"
     gene            complement(7411..7767)
                     /locus_tag="VHK28_01110"
     CDS             complement(7411..7767)
                     /locus_tag="VHK28_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140858.1"
                     /translation="MTTRSIADRLDEAVDELLAGGTPRFDAEVAPLLDTAVRVSSGLP
                     LPAVSERFEARLAGRLRQMSPASRGLDRLRSTTGRQRLIAAGAVSSAAVGMTITAFAV
                     WRTTRRHASPVRLGHR"
     gene            complement(7881..8459)
                     /locus_tag="VHK28_01115"
     CDS             complement(7881..8459)
                     /locus_tag="VHK28_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2140859.1"
                     /translation="MTGPLHEPRPLPSGRDPDRPLVEAAQADRAAFGALYRRYLDRVY
                     AYAFYQLRDHHDAEDVTERVFLAALKALPGYRQQGSTFRAWLFTIAHHTLANAHRSRS
                     RGRTEPLPATFERPAPDDDPAAVVARAEELRRVLTAIDRLPDDRRQVVLLRFVDGLSA
                     REIGQVLDRSPGAVRVLLHRALRELGEQLSPR"
BASE COUNT         1178 a         3134 c         3046 g         1215 t
ORIGIN      
        1 cccacgatca ccaggtcgac gtcccgcggg tcgacgccgg ccaccgccag cgcgtcgcgc
       61 gaggcatgca ccgacagggt agtcgtcgtc tcgtcgtcgg cggcgatgcg ccgctcatgg
      121 atgccggtgc ggctgacgat ccactcgtca gaggtgtcca ccaaggtgga cagctcctcg
      181 ttggtcatca cgcgccgggg cgcgtacatc ccccatccgc tgatcaccgc gttggtcgtc
      241 atgcctgcct ctcctgctta cccgctgggg tcgacttcga tcagcggctg ctggcgtttc
      301 acgagcgtgc cgctctcgac caggatgcga gtcacgcgac ccacaacgtc cgatttgatc
      361 tcattgaaca gcttcatggc ctcgatcagg ccgatgaccg atcccacccc gacctcgctg
      421 ccgtccgaca ggtacgggcg ggcacccgga gacggcgcgg cgtaccacac gccggtcagc
      481 ggagcggtca cgaagcgcag gccgggacca ggctggccgt aggggctggc cggcgccggg
      541 gactcgaccg gtgggggcgc gggcatggtg gccggtgtcg ccgcgggcgc ggccaggacc
      601 gcggctcgcg ggcgagccag gcggatgacc aggtcaccgg cctccacctc cagctcatcg
      661 agcccgctgg cctcgaaggc gggggtgagc aggtcgaccg ccgccagcac cgggtcgacg
      721 gcgcgctcgg tcacgccgcg taccgccgga agacgagcgt ggcgttgtgc ccgccgaagc
      781 cgaacgagtt gctcatcgcg gtccgcacct ccacctgccg cgcctggttg ggcacgtagt
      841 cgaggtcgca gtccggatcg gggttctcgt agttgatggt cggcggcacg atgccgttcg
      901 tgatggccag cacgcaggcg atggcctcga tgccgccggc cgcgcccaga aggtggccgg
      961 tcatgctctt ggtgctgctg accgggatgg ccgccgcccg ctccccgaac agggtcttga
     1021 tggcggccgt ctccgagcgg tcatttggcg gcgtgctagt gccgtgggcg ttgatgtagt
     1081 ccacgtcgcc ggtcgtcagg ccggcatcgg ccagggccag gctcatggag ccgacagcgc
     1141 cgcggccgcc tggtgcgggc agggtgatgt gcgaggcatc gtcggtggcc ccgtagccga
     1201 ccacctcggc atggatcgtg gcgccgcgcg cccgcgcgtg ttccagctcc tccaggacca
     1261 ccagcccggc accatcggcg atcacgaagc cgtcgcgcag cgcgtcgaag gggcgcgacg
     1321 cgcgctccgg ctcgtcgttg caccgggtgg acagcgctcg catggcggcg aaggcggcca
     1381 ccgggatctc gcctatcccc gcctcggcgc caccggccag catgacgtcc gcgtccccgc
     1441 gccggatgag cgacagcgcg ctgccgatgg cgtggttgct gctggcgcag gcgctgaccg
     1501 tcgcatagtt cggcccgcgg gcgtttgcgt agatggcgat gaacccggcc gccatgtccg
     1561 ggatcatggc cgtcaccagg aatggaccca cccggtcggg cccgagctcg tgcgcctcga
     1621 tgatgtcgcg gatcatggtg ttgatgccgc cgatgccggt cccaatgatg gcgccggtgc
     1681 gcatcgcctc atgctcgtcg gcgatggggt aggcgatgcc cgcgtcgctc agcgcctggg
     1741 tggccgcgga ccacgcgtag tgcacgtagc gatcgttgcg ccgcacctcc ttgcgcggca
     1801 tgaccgcgtc ggcgtcgaat ccgcggatct cgccggcgac ccggctcggc accctggctg
     1861 ggtcgaagga ggtgatgggc ccgatgcccg agcgagcgga cttcagtccg tcccagaagc
     1921 tgggcacatc gttgccgagc ggggtcaccg cgcccatgcc ggtgatcacc acccgccgct
     1981 cgccccgctc ggccgagctg ggctgtgcgt aggcggtggg ttgcacgtag gcgtcagtca
     2041 tcggtccctc ctccgagcga ctggtccctg gggaagatga tgctcagcgt cgcctccacg
     2101 gccacctcgc cgtccacgct ggccacgccg cgcacccgcc cgaacatgcc gcgcagcttc
     2161 tcgacggtga cctccaggcg caaggtgtcg ccgggttgca ccatgcgtcg gaagcggcac
     2221 tcgtcgatcc cggcgaacag ccccaacttg ccgcggtagg catccagcgt cgagaccgcc
     2281 aggccggcgg tctgcgccag ggcctcaacc atcagcacgc cgggcatgat cgggcgcgtg
     2341 gggaagtgtc cggtgaactg cggctcgttg gccgtcacgt tcttgatggc caccgcccgc
     2401 tggcccggct cgagctccag gacgcggtcc acgagcagga acgggtagcg gtgcggcagg
     2461 atctccatga tcgccgccgc ctccatgggg agggcgaatg acagggccgg ctcggtcatg
     2521 cgctcatcat cggtcaccgg tcattcaggc gccaccggcc acgaaggccg ccggcccgac
     2581 ccggtccagc aggttggtgc tgacctcggc ctgcaggaac gcggcgttgt ccacgattcc
     2641 gcgatggaag gcgaggttgg tcttgatccc ctcgatccgg aactcggcga gcgcgcgacg
     2701 cgaacgggcc atggccatct cgcgcgtctc gccccacacg atcaacttgg ccagcagcga
     2761 gtcgtagaag ggcggcacct cgtagccgcg gtacaggtgg gtatcgaccc ggacgcccgg
     2821 cccggagggc agctccagct cctcgatggt cccggtctgc ggggcgaagt tgtcgttcgg
     2881 atcttcggcg gtgacgcgga actcgatggc gtggccgcgc aggctgacct gctcctgccg
     2941 cagcgagagg cgctcccccg ccgccaggcg gatctgctcg gcgatcaggt ccacgccggt
     3001 cagcatctcg gtcaccgggt gctcgacctg gatgcggcag ttgatctcga tgaagtagaa
     3061 gctgccgtcc gcgtccagca ggaactccag ggtgcccgcg ctctcgtagc cggcggcgat
     3121 cacggcccgg atggcgaggt cgcgcaggcg ctcgcgggcg gccgggtcca tcgccggcga
     3181 gggcgcctcc tcgatgatct tctggtgccg ccgctgcacg gagcagtccc gctcgccgag
     3241 gtggatgccg ttgccgtgct ggtcgatgag cacctgcacc tccacgtgcc gcgactcctc
     3301 gatccatttc tcgaagtaca cgctgtcgtc gccgaaggcc gcctgcgcct cggagcgggc
     3361 cagcggcagg ctggtctcca actcgcgggg cgagcgcacc agccgcatgc cgcgtccacc
     3421 gccgccgccc gacggcttga tgagcaccgg gtagccggac tgctgggcct gggacagcgc
     3481 atcggccagg tcggtcagga tgccgcggct tcccggcacg gtgggcaggc cgttggcggc
     3541 cagcatgcgg cgcacggcgt acttgctgcc gaagcggtcg aggacctcgg cgcgcggacc
     3601 gatgaacgcc aggtcatggg cggccaccac ctccgcgaag ccggcgtcct ccgacaggaa
     3661 gccgtaccca ggatggatgg cgtcgcagcc cgtgatcagg gccgcgctga ccacggccgg
     3721 ctggttcagg tagctcttgc gcgcctccgc aggaccgatg cacaccgcct cgtcggccat
     3781 gtgtaccgcc agggagtcgc gatccgcctc ggaataggcg accacagctg gcacgcccag
     3841 gtcacggcac gctcgcagga tgcgcacggc gatctcgccg cggttcgcga tcaggatctt
     3901 gccgaacacg ctagaccgat gcctccgccg tggtgctggt gagctccctc agcgtcgcag
     3961 ccaggctcgt cgagtccatc acgttgagcg cgcggagctc gggcgcgatg cgtttcacca
     4021 tgccggtcag cgcctgtccc ggcccgcact ccacgatggt gtccacgccg tcggccacca
     4081 ggcgctgcac ggagcgcacc cactcgaccg gtgcgctgac ctggcgggcc agcagctcac
     4141 ggatctcgtt cggctcacgc accggctggg cattcacgtt ggcgaccagt ggcgggtcgg
     4201 cttcgctcca ggccacgtcg ccgaaggcct cggccagccg ccgtccggca tccgccatga
     4261 acggtgagtg gaacgggccc gatacgttga gcgggatgat ccggcgagcc ccggcggcgc
     4321 tcaggtgcgg ctgcgccgcg gcgagcgccg gccggctgcc cgagatcacg acctggccgg
     4381 gcgcgttgtc gttggccagg accgggtcgt gcccggtgcc ggcctcgctc gcggcgcgct
     4441 cgactgcctg ggctaccgcc tcgcgatcga ggccgatgac cgccgccatg cccccgtccg
     4501 cgccggcatc ggccatcagc gcgccgcgca gcgccaccag ccgcagggcg tcggccagcg
     4561 acagaacccc ggccgctgcc agcgccgcgt actcgccgac cgagtggccg gccatgaagg
     4621 ccgggctcag ggcgcgacca acgcccgcgc tcgcctcctc caacgcacgg aggcatgcca
     4681 gcgaggtggc caccaggcag ggttgcgtct ggcgcgtgtc gttgaggcgt tccgcgggac
     4741 cgtcccagca cagggtggat accggccagc ccagggccgc atcggcctcg gcaaagacgg
     4801 cggccgcgga cggccaggta gtcgccagct cgcgtcccat gcccaccgac tgggatccct
     4861 ggggagagaa gacgattgcc aggctcactc gaccggctcc agctcgatga ggagctgccc
     4921 gtactccacc ggctcgcccg cctcggtgac caggttgcgc accgttccgc cgcggggcgc
     4981 tcgcacgtcg tgccgcacgc cgagcatctc cacgtggccc agctcgtcgc ccttctgcac
     5041 ggccaggccc ggacccaggc cgtccgagta cccgaagaac ccgacggccg gcgaggtgac
     5101 cggggccaga tgcgaggtca ccgccggccc gtggccgggg ttctcgccgt ggggcgcgtg
     5161 ctccacggac aggcgcgggg ccggctcgct ggtcgcgtgg gagccggccg cggtcggtcc
     5221 gggcgaggat gcggcggaca ctttcacccg cagcgtgccc aggcggacct cgatctcgcc
     5281 gaggccgtgg cgctccagcc gctgggtgag gcgtgggacg agatcctccg cggcacgccg
     5341 gacctcgctc agccgctcgt cctggtcggt catgcgcctc cttcaccccc gccgccgccg
     5401 tcgtcgccgc tcggccagat ctcgctccag cgcgggcggc gcgggacgcc gggcagccgc
     5461 aggaaccgtc ccaggcgcct gcgcagcgtc tgctcctcga gggcgcgacc gtccggtgcc
     5521 tccgcctcgc ggaacagccc gatgccccgc agccgagcat agcgcgtcgc gagcagcgcc
     5581 tctggctcga ggcggctcac ccggccgagg gcagccagca gggcggcctt gatggcccgc
     5641 gctgtggccg cgtggtcggt gtgcgctccc tcgccgggct cctcgatgat gcggtcgata
     5701 acgcccagct gcagctggtc ggcggcagtc atgcgcatcg ccgcggcggc ctgcggagcc
     5761 ttgtcgggtg cacgccacag aatggatgcg cagccctccg gcgagatcac cgagtagacc
     5821 gcgttctcga gggccagcac ctcgtcgccg acgccgatgg ccagcgcccc tcccgagccc
     5881 ccctccccga cgatgaccac gaccaccgga acctggagcg cggtcatctg cttgatgctg
     5941 gacgcgatgg cctcggcctg gccgcgttcc tcggccgccg gtccgggaaa cgccccggcc
     6001 gtgtccagca gggtgaccac cggcagcccc agcttctcgg ccagacgcat caagcgctgc
     6061 gcctttcgga agccctcggg gtgcggcgag ccaaagttgc gggtgatgtt cgactcggtg
     6121 tcgcttccct tctgatgacc gatgaccatc accggccgcc cgtcgagcag cgccggtccg
     6181 ccgacgatgg ccgggtcgtc gcggtagctg cggtcgccat ggagctccac gaagtcctcg
     6241 aagatgcgcg gcaccaggtc gagcgtccgc ggacgttgcg cgtggcgggc catctggacg
     6301 cggcgccacg cctcgtcgac ctgctcctgg ctgggcggcg tatcgccgaa ggcgcgcacc
     6361 tgtggcggca gcaggtcgct cacgcctcgc cggtcccgtt attcgggccc gcgccggtcc
     6421 gttccgcgcc tgagcgctcc cgcggtggcg cgccatcacc gtcctgctgg acgccatcca
     6481 ggccgatggt ctcgctgacc gccccgccga cccgctccgc cagccccgag agcacgctga
     6541 tgggacccca accgcgtggc ccgtcctccg ccaggcgctc gtcgacggcc gccaccggca
     6601 gcaggcgcag cagccgggac agcgtggcgc gcagctcggg gcgcgggacc acgaggtcca
     6661 cgaacccgtg ctccagcagg aactcggcgg tctggaagcc atccggcagc tcctcgccga
     6721 cggtgccgct cgccacgcgc gcgccggcga acccgatcag ggcgcgtggc tcggcgagca
     6781 ccacgtcccc caggctggcg aagctggcca ggacgccgcc cgtggtcgga tcggtcagca
     6841 cgctgatgta cgggaccccg gcgaggtcca tgcgggttag tggtgcggtc gtcttggcca
     6901 gctggaggag cgagagggtg ccctcctgca tgcgcgcccc gccggaggcg ctgaccatga
     6961 tggccggcac ccgctcggtg agcgcggcct cgaagcagcg ggcgatcttc tcgcccacca
     7021 cgctccccat gctgccgccc atgaaccgga agtcgaacag gccgatgacc accggccgcc
     7081 catcgatacg gccggtgccc cacaccgccg cctcgcgcag gcccgtcttg acccgcgagg
     7141 cctctatgcg gtcggggtag ggcttctggt cgtggaagcc gagcggatcg gcggactcca
     7201 ggccagcatc gcgctccgcg aagctgtcgg cgtccagcag gaggtcgatg cgctccccga
     7261 tgctcatccg gaagtggtgg ccgcaggtcg ggcacactcg caggttgcgc tccaactgcc
     7321 ggttgaagac ctgggtgtcg cacgagggac acttggtcca ggcatcggcc gggccgtcgc
     7381 ggcgccggag cgggaacttg atgcgcatgc ctagcggtgc cccaggcgca cgggagacgc
     7441 gtgacgccgg gtggtgcgcc acaccgcgaa ggcggtgatg gtcatgccca ccgccgcgga
     7501 cgagacggcc ccggccgcga tcaggcgctg gcgtccggtg gtcgagcgca gcctatccag
     7561 cccgcgcgac gcgggcgaca tctgccggag gcgcccggcg aggcgtgcct cgaagcgctc
     7621 gctgaccgct ggcagcggca gcccgctcga cacgcggacc gccgtatcca gcagcggcgc
     7681 cacctctgcg tcgaaccggg gcgtcccccc ggccagcagc tcgtcgacgg cctcgtcgag
     7741 ccggtcggcg atgctcctcg tcgtcatcgg tcactcctgg gtctggtggg aagggctcat
     7801 cctaggcgaa caccgcgagg accccaaacg ttacgcggcc cgcgcgccgc ggctgcccgg
     7861 atcgtcgggg cgaagcccgt ctagcgcggg ctgagctgct cgccgagctc gcgcagggcc
     7921 cggtgcaaca ggacccgcac cgcaccgggt gagcggtcca gcacctggcc gatctcgcgg
     7981 gcgctcaacc cgtccacgaa gcgcagcagc accacctgtc ggcggtcgtc gggaagccgg
     8041 tcgatggcgg tgagcaccct gcgcagctcc tcggcacgcg ccaccaccgc ggctggatcg
     8101 tcgtccggag ccgggcgctc gaaggtggcc ggcaacggct ccgtgcgccc ccgggaccgg
     8161 ctgcgatggg cgttggcaag cgtgtggtgc gcgatcgtga acagccaggc ccggaaggtg
     8221 gatccctgct ggcggtagcc agggagcgcc ttcagcgcgg ccaggaagac gcgctcggtg
     8281 acgtcctccg cgtcgtggtg gtcgcgcagc tggtagaagg cgtaggcgta gacccgatcc
     8341 aggtagcgcc ggtagagggc cccgaacgcc gcccggtcgg cctgcgcggc ctcgaccagc
     8401 ggccggtccg gatcgcggcc ggaaggcagc ggccgtggct cgtgcagggg gccggtcacg
     8461 ccggcaccac gtcgcgcacg gcaaccacct caccgacgtg cagcgcatgg cgctcgccgt
     8521 cggcctccag cagcagcgcg ccgtcatcgt cgatacccac cacggtgccg ctc
//
LOCUS       DATACA010000051         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_248315, whole genome shotgun sequence.
ACCESSION   DATACA010000051 DATACA010000000
VERSION     DATACA010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248315"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="hydA"
                     /locus_tag="VHK28_01120"
     CDS             <1..1129
                     /gene="hydA"
                     /locus_tag="VHK28_01120"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX2140860.1"
                     /translation="GTTTIIDFAVQPRGGSLRQGLDAWHEKAEGNACIDYGFHMIVSD
                     VRDDVLAEMDTLVDEGVTTFKLFTAYPGVFFSDDGAIFRAMQQSAKNGGLIMMHAENG
                     LAIDVVAGQEFAAGNTDPYYHGTSRSPIMEGEATNRVIRLAEVAGAPVYIVHLSARHA
                     LNEVRRARDEGLPAFAETCPQYLFLSLEDLGNGFEGAKYVCSPPLRPADHQEDLWLGL
                     LKDDLQVVSTDHCPFDFHEQKELGRGDFRKVPNGLPGVEDRLDLLHHGGVVGGRLTPN
                     RWVEVISTAPARLFGLPQKGAVAVGMDADLVVYDPNRKRTISARTHHMDVDYSVYEGM
                     EITGGSDVVLSRGRIVVDGDEWLGEKGGGRFLRRQRSSFLH"
     gene            1199..1888
                     /locus_tag="VHK28_01125"
     CDS             1199..1888
                     /locus_tag="VHK28_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140861.1"
                     /translation="MITDDRWPAGRTPTRRRLFGPRGAALAGATALVMVLAACGEAAP
                     PSVSPSASVTPEATPSPSEPMGGTAGANPSLPSSVPELEAVIPDQVAGMSLEKSSMRG
                     EDFIDSQEADELTREFLRQIEVDPEDLAVAVGFGLDRDTGTGVGVLVFNAQGADPDAL
                     VRVFKETSDAERDTPLEWESVTVGGKEVERTTDPLQGDQVVHLYGHEDTLFLVSATSD
                     EIAAEALEALP"
     gene            1912..2808
                     /locus_tag="VHK28_01130"
     CDS             1912..2808
                     /locus_tag="VHK28_01130"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2140862.1"
                     /translation="MSSTLPDGPPRPAAIVVNERAGSAATARVRRSVELVRRALDADL
                     HVAATRDPAELAAWLRERIAPYSTVVVAGGDGSLGVAYNVLAAADVTLGWIPAGTGNA
                     TAHLLNLPRDPDRLAGVLARGVARPLDLVSVDGHLALFAGVGWDAVVAGRYAAAGAHG
                     LLGWGAAIARSLPDLRRPAAVLVEADGKVIHSGPMELLVVGTTPWWGRGLLVNPGARP
                     DAGQLTLRIYPASVPRFALDAVRWLARRRPSVAPIPARSVTVRSLDGAPLPVEADGDA
                     LEALGEWHFEVRPAAVRLIGHW"
     gene            3023..>3258
                     /locus_tag="VHK28_01135"
     CDS             3023..>3258
                     /locus_tag="VHK28_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140863.1"
                     /translation="MLSALALVLSTAGTAIAAPPEGRSANGPAPKSDNLPNPLGNRQA
                     TLKQQAQEMILKGQAKAQGKNKVVKLAPGQFVELA"
BASE COUNT          493 a         1126 c         1150 g          489 t
ORIGIN      
        1 cgggaccacc accatcatcg acttcgcggt gcagccccgc ggtggctccc tgcgccaggg
       61 cctggacgcc tggcacgaga aggcggaggg caacgcctgc atcgactatg ggttccacat
      121 gatcgtgtcc gacgtccgcg acgacgtgct ggccgagatg gataccctgg tggacgaggg
      181 tgtcaccacc ttcaagctgt tcaccgccta cccgggcgtc tttttctcgg atgacggcgc
      241 catcttccgc gccatgcagc aatcggccaa gaacggcggc ctgatcatga tgcacgccga
      301 gaacggcctg gccatcgacg tcgtcgccgg gcaggagttc gccgccggca acaccgatcc
      361 ctactaccac ggcacgtcgc gctccccgat catggagggc gaggccacca accgcgtcat
      421 tcggctggcc gaggtggccg gtgcgccggt ctacatcgtc cacctctccg cccggcacgc
      481 cttgaacgag gtgcggcgcg cccgggacga gggcctgccg gccttcgccg agacctgtcc
      541 gcagtacctg ttcctctcgc tggaggacct tggcaacggc ttcgagggcg ccaagtacgt
      601 ctgctctccg ccactgcgcc cggccgatca ccaggaggac ctgtggctgg gcctgctgaa
      661 ggatgacctg caggtggtct caaccgacca ctgcccattc gacttccacg agcagaagga
      721 gctgggccgc ggcgacttcc gaaaggtgcc caacgggctg ccgggcgtcg aggaccggct
      781 cgacctgctg caccacggcg gggtagtcgg aggccggctc acgccgaacc gatgggtgga
      841 ggtcatctcg accgcacccg ctcgcctgtt cggcctccca cagaagggcg ccgtggcggt
      901 gggcatggac gcggacctgg tggtctatga cccgaaccgc aagcgcacga tctcggcccg
      961 gacgcaccac atggacgttg actactcggt ctacgagggc atggagatca ccggcggctc
     1021 ggacgtcgtg ctctcgcgcg gccgcatcgt ggtggacggc gacgagtggc tgggcgagaa
     1081 gggcggcggc cgcttcctgc gaaggcagcg gagctccttc ctgcactaag agccgctgcc
     1141 gacggcacgc accgtgcgag cccgcctccg cagcacatga agggcggagg tatccagcgt
     1201 gatcacggat gaccgatggc cagcgggccg cacgccgacg aggcgtcgcc tctttggccc
     1261 ccgcggcgcg gcattggcgg gggccacggc gctggtcatg gtcctggccg cctgcggcga
     1321 ggccgcccct ccatcggtct ctccatctgc cagcgtcacg ccggaagcca cgccgagccc
     1381 gtcggagccg atgggtggga cggcaggagc caatccctcg ctgccatcca gcgtcccgga
     1441 gctggaggcg gtcatcccgg accaggtggc cggcatgtcg ctcgagaagt cttccatgcg
     1501 tggcgaggac ttcatcgact cgcaggaagc ggatgagctg acccgcgagt tcctgcgcca
     1561 gatcgaggtc gatccggagg acctggcggt tgcggtcggg ttcggcctgg atcgggatac
     1621 agggaccggg gtgggcgtgc tggtcttcaa cgcccagggc gcggatccgg acgccctggt
     1681 gcgcgtcttc aaggagacga gcgatgccga gcgggacacc ccgctggagt gggagtcggt
     1741 aaccgtcggc ggcaaggagg tggaacgcac cacggacccg ctgcagggcg accaggtcgt
     1801 gcacctctac gggcacgagg acaccctctt cctggtgagc gccacctccg acgagatcgc
     1861 cgcggaggcg ctggaggcgc ttccctagcg gatcgggcgt agagtcggct ggtgagtagc
     1921 accctgccgg acggcccgcc gcgtcccgca gccatcgtcg tcaacgaacg cgccgggtcc
     1981 gcagcgaccg cccgcgtccg gcgatccgtg gagctggtgc ggcgcgcgct ggacgccgac
     2041 ctgcacgtgg cggccacccg cgatccagcc gagctggctg cctggcttcg cgaacgcatc
     2101 gcgccctaca gcacggtcgt ggtggccgga ggggatggct ccctgggcgt cgcctacaac
     2161 gtcctggccg ccgccgacgt gacgctgggc tggatcccgg cggggacggg caacgccacc
     2221 gcccacctcc tcaacctgcc gcgggatccg gaccggctcg ccggagtgct ggctcgcggc
     2281 gtggcgcgcc cgctcgacct cgtgtcggtg gacggccatc tggccctgtt cgccggcgtg
     2341 ggctgggacg cggtggtcgc tggacgctac gccgcagccg gggcccacgg gttgctggga
     2401 tggggcgccg cgatcgcccg gtcgctaccg gacctgcggc gtcccgcggc ggtgctggtg
     2461 gaggcggacg ggaaggtcat ccattcggga ccgatggagc tgctggtcgt cggcaccacg
     2521 ccatggtggg gacgaggcct gctggtcaac ccaggggcgc gccccgacgc cgggcagctg
     2581 acgttgcgca tctaccccgc gtcggtgccg cgtttcgcgc tggacgccgt tcgctggctg
     2641 gcgcgacgcc ggccctcagt ggcgcccatc ccggcccgct ccgtcaccgt ccgctcgctc
     2701 gacggagctc cgctcccggt cgaggccgat ggcgacgcgc tcgaggccct cggggagtgg
     2761 cacttcgagg tccggcccgc ggccgtccga ctcatcggtc attggtgatc cggcgcaccg
     2821 catagcgtcg ggattcgttg cagcacgcaa agtgcccggc gtaaagttcc cttcacccaa
     2881 tcattaccgg cgtgaaatgc gccggtcctg cgctgcacgg cgacgccacg aggggaggtg
     2941 ttgcgagagt cgtcgatcgg cagcacgcgc ctgatttgga tgaagattgc accggagaat
     3001 tgagttgcga aagctcatca cgttgctcag cgcgctggcg ttggtgctca gcaccgccgg
     3061 caccgcgata gctgctccgc ccgagggccg gtcggccaat gggccggcac cgaagtccga
     3121 taacctcccg aatccgctcg ggaaccggca ggctaccctt aagcagcagg cccaggagat
     3181 gatcctcaag ggccaggcca aggcccaggg caagaacaag gtcgtcaagc tcgctcccgg
     3241 ccagttcgta gagctggc
//
LOCUS       DATACA010000052         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1534190, whole genome shotgun sequence.
ACCESSION   DATACA010000052 DATACA010000000
VERSION     DATACA010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534190"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VHK28_01140"
     CDS             <1..580
                     /locus_tag="VHK28_01140"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX2140864.1"
                     /translation="LLSASDQPGGLLGAELFSDGTGCEGIIIPAGGSVHPASAETVAA
                     NRHTLHMQGSEVYKYATRQLVDSAANAIRDAGLTVDDIDQFVFHQANLRIIKSVEKSL
                     GIDEDKVFVNIEKYGNTSAASVPMALVEAVAAGRIKPGDRVLMVAFGAGYTAGAAVVE
                     WTADPARAFMAPGEQTRLGRPQVQEPALAGAR"
     gene            581..1336
                     /gene="fabG"
                     /locus_tag="VHK28_01145"
     CDS             581..1336
                     /gene="fabG"
                     /locus_tag="VHK28_01145"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX2140865.1"
                     /translation="MFDLTGRVALVTGGSRGLGRAIALALAEQGADVAVNYRGNREAA
                     EETVERIRSAGRRAEPIQGDTSGGREACAAIVKAALDAFGMVDILVNNAGITRDNLMM
                     RMGTDEWDEVLATNLSGPFWMTRAMSRPMMKARYGRIINMSSAAGRMGNAGQANYAAA
                     KAGLIGLTKTTARELASRGITCNALAPGLITTDLTADLPEAATEALKAMTPIGYIGSV
                     EDVAAAAVYLASDEARYVTGQVLGVDGGIVMGS"
     gene            1449..1880
                     /gene="sodN"
                     /locus_tag="VHK28_01150"
     CDS             1449..1880
                     /gene="sodN"
                     /locus_tag="VHK28_01150"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX2140866.1"
                     /translation="MLTLRTLLRPSRRVHAHCDIPCGVYDPEQARIEAESCLKIIEKY
                     QGNEDKVFRARCIHVKEERAELVKHHLDVLWHDYFKPEHLEKYPDLHETFWKATKQAS
                     KVKHTVDAGAAQELLTMIDRIDEMWKATGGPEKTRVNGRPG"
     gene            1896..2396
                     /gene="sodX"
                     /locus_tag="VHK28_01155"
     CDS             1896..2396
                     /gene="sodX"
                     /locus_tag="VHK28_01155"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HEX2140867.1"
                     /translation="MLTQRGPIRPLFLALGTAAGLLVASWVLRRTDAVEVVGGSMSPT
                     LLPGDRLLVESWTYARRAPRRGEVVLAADPRSPARELIKRVAAVHGAHVDLRGDAAAA
                     STDSRHFGRLRIHDVRWRVGLRYWPLRRIGALPPPPAPLLLEPPGGEPACAAFEALIA
                     GDQPDL"
     gene            complement(2531..2896)
                     /locus_tag="VHK28_01160"
     CDS             complement(2531..2896)
                     /locus_tag="VHK28_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140868.1"
                     /translation="MAAEPIVYCLTRDRELATALEVRLSAALAFFYNDAARLHQAIVL
                     RAPDAVVVDTGAIREEYGDAGLGPVIAFIRRRAPEARIAVRPHAGGEWLVAAEAGEGV
                     RMLPPEAGACVEAIADFCA"
BASE COUNT          494 a         1105 c         1071 g          402 t
ORIGIN      
        1 gctgctgtcc gcctccgacc agcccggcgg cctgctgggc gccgagctgt tcagcgacgg
       61 caccggctgc gaggggatca tcatccccgc cggcggctcc gtgcatccag cctcggccga
      121 gacggtggcc gccaaccggc atacgctgca catgcagggc agcgaggtct acaagtacgc
      181 cacccggcag ctggtggact ccgcggcgaa cgccatccgc gacgccgggc tgacggtgga
      241 cgacatcgac cagttcgtct tccaccaggc caacctgcgc atcatcaaga gcgtcgagaa
      301 gtcgctgggc atcgacgagg acaaggtctt cgtcaacatc gagaagtacg gcaacacatc
      361 ggcggcatcg gtccccatgg cgctggtgga ggcggtggcc gccggtcgca tcaagcccgg
      421 cgaccgggtg ctgatggtgg ccttcggcgc cggttatacc gccggcgctg cggtggtgga
      481 gtggaccgcc gacccggcgc gtgccttcat ggcccccggc gagcagacgc gcctcggcag
      541 gccgcaggtg caggagcccg ccctggccgg agctcgatag atgttcgacc tgaccgggcg
      601 ggtggcgctg gtcaccggcg gcagccgcgg cctgggacgc gccatcgccc tggccctcgc
      661 cgagcagggc gcggacgtgg cggtcaacta ccgcggcaac cgcgaggcgg ccgaggagac
      721 ggtcgagcgc attcggtcgg ctggccgcag ggcggagcca atccagggcg acacctccgg
      781 tgggcgggag gcgtgcgcgg cgatcgtcaa ggcagccctg gacgccttcg gcatggtcga
      841 catcctggtc aacaacgctg gcatcacccg ggacaacctg atgatgcgga tggggaccga
      901 tgagtgggac gaggtgctgg ccaccaacct gtccggcccg ttctggatga cccgcgccat
      961 gtccaggccc atgatgaagg cccgatacgg acggatcatc aacatgtcgt ccgcggccgg
     1021 ccgcatgggg aacgccggcc aggccaacta cgccgcggcc aaggcggggc tcatcgggct
     1081 gaccaagacg accgcgcgcg agctggcgag tcggggcatc acctgcaacg ccctggcgcc
     1141 gggactgatc accaccgacc tgaccgccga cctgccggag gccgccaccg aggccctcaa
     1201 ggcgatgaca cccattggct acatcggctc ggtcgaggac gtggccgccg ccgccgtcta
     1261 cctggcctcg gacgaggccc gctacgtcac cggccaggtg ctgggcgtcg acggcggcat
     1321 cgtgatgggg agctgacgcc gcctgctcat tcgacgagcg agcgcatgct cccggcatgg
     1381 ggattgctgc gaaaatgggg ccagccaggc ccaccatcgg ttcacccgac acagcgagag
     1441 gagactccat gctcaccctt cgcaccttgc tgcgcccatc ccggcgcgtc cacgcccact
     1501 gcgacatccc atgcggcgtc tatgacccgg agcaggcccg catcgaggcg gagtcgtgcc
     1561 tgaagatcat cgagaagtac cagggcaacg aggacaaggt cttccgcgcc cgatgcatcc
     1621 acgtcaagga ggagcgcgcc gagctggtga agcaccacct ggacgtgctg tggcacgact
     1681 acttcaagcc ggagcacctc gagaagtacc cggacctgca cgagaccttc tggaaggcca
     1741 ccaagcaggc ctcgaaggtc aagcacaccg tggacgccgg ggcggcccag gagctgctga
     1801 ccatgatcga ccgcattgac gagatgtgga aggccaccgg cggcccggag aagacgcgcg
     1861 tcaacggccg cccgggctga cccgggccca gaccgatgct gacgcagagg ggcccaatcc
     1921 ggcccctctt cctcgcgctg gggaccgcgg ctggcctgtt ggtcgcgtcg tgggtgctgc
     1981 gccggaccga tgccgtcgag gtggtcggcg gctccatgag cccgaccctg ctccccgggg
     2041 accggctgct ggtcgagtcg tggacctacg cgcgccgcgc gccgcgccgg ggcgaggtgg
     2101 tgctggccgc ggacccgcgc tccccggctc gcgagctcat caagcgcgtg gccgccgtgc
     2161 acggtgcgca tgtcgacctg cggggagacg cagccgccgc cagcaccgac tcacggcact
     2221 tcggccgcct ccgcatccac gacgtccgtt ggcgggtggg gctgcgctac tggccgctgc
     2281 ggcgcatcgg ggcattgccg ccgccgccgg cgccgctgct gctcgagccg ccgggcgggg
     2341 agccagcctg cgccgccttc gaggcgctga tcgccgggga ccagcccgat ctataggggc
     2401 accctgcaca cgaacgcccc gaaaccctca attcatgcgc tcgctacccc gatacaccga
     2461 tcaggcgaca catgggcatc ggttcctgcg cagcctgcac ctatagctcg gtcagccacg
     2521 cgtcccgtgg tcaggcgcag aagtcggcga tcgcctccac gcacgcgcct gcctccggag
     2581 ggagcatgcg gacgccctcg ccggcctcgg ccgccaccag ccactctccc ccggcatgcg
     2641 gccgcaccgc gatgcgcgcc tccggcgccc gccgacggat gaaggcgatc accggtccca
     2701 ggccggcgtc cccgtactcc tcgcgaatgg cgccggtgtc gaccaccacc gcatccggag
     2761 ctcggaggac gatcgcctgg tgcaggcgcg ccgcgtcgtt gtagaagaag gcgagggccg
     2821 cgctcagacg tacctcgagc gcggtcgcga gctcgcgatc gcgggtgagg cagtagacga
     2881 tcggttcggc ggccatgggc accgaagggt agcagccgct ctcggcggta caatgagggg
     2941 ccgatgaccg ccacccccgc tcgtaccgat ccacgacgcg acctgacgac cggccgccac
     3001 cagcaccttg gcagtctgct cgggcgcgtc tgggcggtcg acgccgtcgg cgccgacgag
     3061 cgagcggcga gc
//
LOCUS       DATACA010000053         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_570340, whole genome shotgun sequence.
ACCESSION   DATACA010000053 DATACA010000000
VERSION     DATACA010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570340"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VHK28_01165"
     CDS             complement(<1..262)
                     /locus_tag="VHK28_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140869.1"
                     /translation="MTASAYPTERRKKPEYWPIISGYTPKFQVSWYPCPSEPVCRLTG
                     SLGQGYQLTWNFGVYPEMIGQYSGFFLRSVGYALAVTALCLLI"
     gene            240..887
                     /locus_tag="VHK28_01170"
     CDS             240..887
                     /locus_tag="VHK28_01170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2140870.1"
                     /translation="MGYALAVTTLCLLIGFPLAYTIAFRGGRAKSILLLLVILPFFTS
                     FIIRTLAWKLILQDNGFVLGTFKEIGILDAGFRVLATPISVISGLTYNFLPFMVLPLY
                     VALEKIDPRLIEAATDLYASRWQAFRRVTLPLAMPGIFAGSLLVFIPAVGDYVNALIL
                     GSPQTQMIGNVIQRLFLNTNAYPEASALGFLMMAAVLVMITAYARMVGSEELMDR"
     gene            884..1762
                     /locus_tag="VHK28_01175"
     CDS             884..1762
                     /locus_tag="VHK28_01175"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2140871.1"
                     /translation="MSAPAVPIGTPATGPVAINRPSLRTRLGRLVLPVYTGVAVLYLF
                     LPIAVMIAFSFNDPRGRQNITWQGFTLENYATIWGRPDIIEPMVRSMTVAIISTLLAT
                     IFGTLIGLALTRYQFRARGPMSLFIFVPMATPEIIMGASLLAMFVSVGVARDLTTIVI
                     AHVAFNISYVTVTVRARLAGFNRTLEEAAMDLGADEWTTFRKVTLPLIFPGILAAALL
                     AFALSIDDYVITSFVAGQATTFPLWVFGVSRLGVPPEVNVLGTLIFGIALLFIAIKVL
                     TQRRRATTEAVAAPAR"
     gene            1759..>2346
                     /locus_tag="VHK28_01180"
     CDS             1759..>2346
                     /locus_tag="VHK28_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2140872.1"
                     /translation="MTATRAAAHAVAVHPGREGDFRTYRIGEAARLVDTSPSALRSWE
                     RQGLVRPRRTRSGYRLYTDSDLAVLRRVRHMRSERVSAPGIRRLIAATPGGTTDGEAR
                     RRRLDGRRVRSLRLEQGLSLREAAARTGLSVSFLSAVERDASGVSVASLQRLTAAYGV
                     TLLDLFSPATVGQLVRPAQRRVLELQGGVRIEQLAS"
BASE COUNT          332 a          847 c          772 g          395 t
ORIGIN      
        1 cgatcagcag gcacagcgcg gtgacggcca gcgcgtaccc taccgagcgc aggaagaagc
       61 cggagtactg gccgatcatc tcggggtaga ccccgaagtt ccaggtcagc tggtagccct
      121 gccccagcga gccggtcagc ctgcagaccg gttcgctggg gcaggggtac cagctgacct
      181 ggaacttcgg ggtgtacccc gagatgatcg gccagtactc cggcttcttc ctgcgctcgg
      241 tggggtacgc gctggcggtc accacgctgt gcctgctgat cggcttcccg ctggcctaca
      301 ccatcgcctt ccgtggcggc cgggccaaga gcatcctgct gctgctggtc atcctgccgt
      361 tcttcaccag cttcatcatc cggaccctgg cctggaagct gatcctgcag gacaacggct
      421 tcgtgctggg caccttcaag gagatcggca tcctggacgc cggcttccgg gtgctggcca
      481 cgccgatatc ggtcatcagt ggcctgacct acaacttcct gccgttcatg gtgctgccgc
      541 tgtacgtggc gctcgagaag atcgatccgc ggctgatcga ggctgccacc gacctgtacg
      601 ccagccgctg gcaggcgttc cggcgggtca cgctgccgct tgccatgccc ggcatcttcg
      661 ccggctcgct gctggtcttc atcccggcgg tgggtgacta tgtcaacgcc ctgatcctgg
      721 gaagccccca gacgcagatg atcggcaacg tcatccagcg cctcttcctg aacaccaacg
      781 cctacccgga ggcatccgcc ctgggcttcc tgatgatggc cgcggtgctg gtcatgatca
      841 cggcctacgc ccgcatggtc ggtagcgagg agctgatgga ccgatgagcg cgccggccgt
      901 cccgatcggc accccggcga ccggcccggt cgccatcaac cgcccatcgc tgcgcacgcg
      961 cctggggcgc ctggtcctgc ctgtctacac cggggtggcc gtcctctacc tgttcctgcc
     1021 catcgcggtc atgatcgcct tcagcttcaa cgatccgcgc ggccggcaga acatcacctg
     1081 gcagggcttc accctggaga actacgccac catctggggg cggccggaca tcatcgagcc
     1141 catggtccgc agcatgacgg tggccatcat ctccacgctg ctggccacca tctttgggac
     1201 cctgattggc ctggccctga cccgctacca gttccgggca cgcggaccga tgagcctgtt
     1261 catcttcgtg ccgatggcca cgcccgagat catcatgggt gccagcctgc tggccatgtt
     1321 cgtctcggtg ggcgtggcgc gtgacctgac caccatcgtg atcgcccacg tggcgttcaa
     1381 catcagctac gtgacggtca ccgtgcgcgc gcggctggcc ggcttcaacc gcaccctgga
     1441 ggaggccgcc atggaccttg gagcggacga gtggaccacc ttccgcaagg tgaccctgcc
     1501 actcatcttc ccgggcatcc tggccgcggc gctgctggcc ttcgcgctgt ccatcgacga
     1561 ctacgtgatc acctccttcg tggccggcca ggcgaccacc ttcccgctat gggtcttcgg
     1621 ggtcagccgc ctgggcgtgc cgccggaggt gaacgtgctc ggcacgctga tcttcgggat
     1681 cgcgctgctc ttcatcgcga tcaaggtcct gacccagcgg cggcgagcca cgaccgaagc
     1741 ggtcgcggcg ccggcgcgat gaccgccacc agggcggccg cgcacgccgt cgccgtccac
     1801 cccggccggg agggcgattt ccgcacctac cgcatcgggg aggcggcgcg attggtcgac
     1861 acctcgccct ccgccctccg gtcgtgggag cggcagggac tggtgcggcc gcgccgaacg
     1921 cgcagcggct accggctcta caccgactcg gacctggccg tgctgcgccg tgtgcgccac
     1981 atgcgcagcg agcgggtcag cgcgccgggc atccggcgcc tgatcgcggc cacgcccggc
     2041 ggcacgaccg atggcgaggc gcgccgccgc cggctggacg gacgccgcgt gcgaagcctg
     2101 cgcctggaac aggggctctc gctacgcgag gccgcggcac gcaccggcct gtcggtcagc
     2161 ttcctgtcgg cagtcgagcg ggacgcctcg ggcgtcagcg tagccagcct ccagcgcctg
     2221 acagctgcat acggggtcac gctgctcgac ctgttcagcc cggcgacggt cgggcagctg
     2281 gtgcggcccg cccagcggcg cgtcctggag ctccagggcg gggtgcggat cgagcagctg
     2341 gcgtcg
//
LOCUS       DATACA010000054         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_891996, whole genome shotgun sequence.
ACCESSION   DATACA010000054 DATACA010000000
VERSION     DATACA010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891996"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="argS"
                     /locus_tag="VHK28_01185"
     CDS             <1..1200
                     /gene="argS"
                     /locus_tag="VHK28_01185"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX2140873.1"
                     /translation="RVLEGVGHHVTREYYFNDFNAQVRNLGLSVLARRRNEPIPEDGY
                     HGEYVAELADDVPPATWEAATASGADPGAVLGRWASERVRAGIEASLASLGVRFDVWT
                     SEGSLHDDGWVDRAVEQLRAAGHIYEQEGATWFRSTAFGDDKDRVVYRSNGQPTYFAS
                     DIGYLGQKFSRGFNELIYLWGEDHHGTVARNRAAAEALGFDSSSVHWLLMAWVRFVRD
                     GREIGMSKRAGEFISLDELLAEIGADAARWFFGSRAHTTGIDFDIELAKKQSAENPVY
                     YVQYAHARCASILRNARERGVSPDASRAGDLLHHPAEQALLRRMLELPDVVADAAERL
                     ETHEITRFLLEVAQLFSQFYRDCRVLSDEPDEAHLSASRLALTDASRQVLANGLGLLG
                     ITAPESM"
BASE COUNT          235 a          511 c          529 g          233 t
ORIGIN      
        1 cgcgtcctgg agggcgtggg gcatcatgtc acgcgcgagt actacttcaa cgacttcaac
       61 gcccaggtcc gcaacctggg tctctcggtc ctggcgcgcc gtcgcaacga gccgatcccc
      121 gaggatggct accacggcga gtacgtggcc gagctggcgg atgacgtccc gcccgctacc
      181 tgggaggccg ccaccgcatc gggcgccgac cccggcgccg tcctgggtag gtgggccagc
      241 gagcgggtgc gtgccggcat cgaggccagc ctggcgtcgc tcggcgtgcg cttcgacgta
      301 tggaccagcg aagggtcgct gcacgacgac ggatgggtgg atcgagccgt ggagcagctg
      361 cgggccgcgg gtcacatcta cgagcaggag ggggccacct ggttccggtc caccgcgttc
      421 ggtgacgaca aggaccgcgt ggtctatcgg tccaacgggc agccgacgta cttcgccagc
      481 gacatcgggt acctgggcca gaagttcagc cgggggttca acgagctgat ctacctgtgg
      541 ggggaggacc atcacggcac ggtggcgcgc aaccgggcgg cagccgaggc actcggcttc
      601 gactcctcat cggtgcactg gctgctgatg gcctgggtgc gcttcgtgcg cgacgggcga
      661 gagatcggca tgagcaaacg cgccggcgag ttcatcagcc tggacgagct gctggcagag
      721 atcggggccg acgcggcacg ctggttcttc gggtcccgcg cacacacaac cggcatcgat
      781 ttcgacatcg agctggcgaa gaagcagagc gccgagaacc cggtctacta cgtgcagtac
      841 gcccacgccc gatgcgcctc catcctgcgc aatgcgcgcg agcgaggggt ctcgcccgat
      901 gcctcccgcg ccggcgacct gctgcatcac ccggccgagc aggccctgct gcgccgaatg
      961 ctcgagctgc ccgacgtggt cgccgacgcc gccgagcggc tggagaccca cgagatcacc
     1021 cgcttcctgc tggaggttgc gcagctcttc agccagttct atcgggactg ccgtgtcctc
     1081 tccgacgagc cggatgaggc gcacctgtcc gcctcgcgcc tggccctgac cgatgcctcc
     1141 cgccaggtgc tggccaacgg cctggggctg ctggggatca ccgctcccga aagcatgtag
     1201 cctccgccgt gcgcgctgcc ggtagccaca gggtggttgc aacggccaag aagccatcgg
     1261 gatcggcgct tcgatggccg tggcgtccgc tcggcgggtg tggcagccca tgaagcgctt
     1321 gacttcaggc cctcgctggc tgttggagcc atccggtgca cgcagcccgg gatttcggcg
     1381 ggtgatagcc acgggatggc cgcaacgccc aagaagccat cgacttccgg gcctcgatgg
     1441 ccgcggcgat cacccggcgg ttgagacggc caacgaaggg ccgatcctcg atcctcgctg
     1501 gccgttgg
//
LOCUS       DATACA010000055         5032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1534632, whole genome shotgun sequence.
ACCESSION   DATACA010000055 DATACA010000000
VERSION     DATACA010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534632"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VHK28_01190"
     CDS             complement(<1..782)
                     /locus_tag="VHK28_01190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013473341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2140874.1"
                     /translation="MPLPHDSDPKLAQYAHPERLVTTAWLAEHRDDSQLVVVESDEDI
                     LLYDTGHIPGAVRVDWHTELNDPVTRDYVDSAGFARLMSAKGIGRDSTVVFYGDKSNW
                     WAAYAIWVFSLFGHPDLRLLDGGRAKWEAEGRPMTTDVPDRPTTDYPTVDRDDARIRA
                     FRDDVLAHLGRPLVDVRSPDEFTGRLLHMANYPQEGAMRGGHIPGARNVPWARAANED
                     GTFKSRDELEMLYQRELGLGPADEVIVYCRIGERSSHTWFALT"
     gene            862..1311
                     /locus_tag="VHK28_01195"
     CDS             862..1311
                     /locus_tag="VHK28_01195"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2140875.1"
                     /translation="METDDRSLPGEREAWDRLHAAIDRLTPETAGATGYYVEGWTAKD
                     AVAHLGTWMAEGAQVLRRIAAGTFLAGELDVDAANARFLDAMRDVPLETVHLQADAAH
                     AELLRAYAELPRVTEDAAYWIRKAGPEHVAEHLPRLEEWIDEMTGRR"
     gene            complement(1325..1861)
                     /locus_tag="VHK28_01200"
     CDS             complement(1325..1861)
                     /locus_tag="VHK28_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013019061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX2140876.1"
                     /translation="MARLGREVQPFGKLHDLPIELDQRPRAESCTILNEILADSTILY
                     ALYKKHHWNVAGPTFYQLHLLFDKHAEEQLELIDLIGERVQTLGGIAVGDPRHAAELT
                     TIERAPDGAEEVPAQITRLLEAHRIIIGKVRAGIEKTEKNGDYGSNDLLMGDVLRRHE
                     LQVWFLSAHLVDVPLLQD"
     gene            complement(2047..2451)
                     /locus_tag="VHK28_01205"
     CDS             complement(2047..2451)
                     /locus_tag="VHK28_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140877.1"
                     /translation="MERTLKTAAPGPEYRPGVCNIGPAEIAARRRTGHVGLAATTGLL
                     ALLLAMEVDAAWRLLTFFPAVGMAAGYLQAAFRFCAGFGWLGVFNFGVRLRDTTSVQD
                     AEARAADRRKALLISALSGGIAALVALVALLI"
     gene            2515..3849
                     /locus_tag="VHK28_01210"
     CDS             2515..3849
                     /locus_tag="VHK28_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2140878.1"
                     /translation="MNAPPDSIPAVLPQQPPFTIRARLLTPLRAGGLRWEPDAALRVD
                     AQGRLEHIGHGSASDGAFDLRPWVVMPGLVDLHAHLPQLPNAGVGAGLHLLDWLERYI
                     FPLERGFDRGVANELAPAAFRAFAHAGTTTVVAYGAIWPDSLDACFQAAETHGIRAVI
                     GKVMMDRLSYDTARQPDEVLELSVRQSAELCETWHGRDAGRLSYAWTPRFAVSCSPQM
                     LAESARRAEAAGAYWQTHLAEDRGEVEEVRRLFPETTDYLDAYDRAGGLTSRAILAHA
                     IYLSDREVARLAESGAVVAHCPSSNLFLASGAMPLARYLGEGVKVGLGSDVAAGPDVS
                     LFAAMRAGAYTQNGLHAMGLADTPAMAPGEWLRLGTLGGAEGLGLGDRIGSLEVGKEA
                     DLIAVDASMTLPLGGMRAISADGPDEILSRLIYRCHPDMVQAAWVRGRLLPA"
     gene            3890..4279
                     /locus_tag="VHK28_01215"
     CDS             3890..4279
                     /locus_tag="VHK28_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2140879.1"
                     /translation="MDRILQILVNAGALYVAVLIVPGLDFEQTTDDWWWKFLLVAVAF
                     SLVNSYLRPVLRILTFPISLVTMGLFLLVINALMLLLTGLISDQLDLGLQVADFWAAL
                     LGSIVISLVGWILSMVVGAARMPGKVL"
     gene            4302..4979
                     /locus_tag="VHK28_01220"
     CDS             4302..4979
                     /locus_tag="VHK28_01220"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEX2140880.1"
                     /translation="MSTDRVHPLVAFVFAATVALAILLTGPMLLFAPPFVSFMQARHD
                     VAARLETDQATVDGATGIMLRDLLLDGDFTVSIHDSGEPVLDPTERSHMRDVGAVVRT
                     LLLIDVVSLLMVALAARRLRRDPARRGRLVIMAAAGIGLAALVLAAFFALAFDVAFAA
                     FHALFFDPGTWQFGPDSNLLRFFPQPFWFEIALVAGGSIVLGALLASALAWRDIAGSH
                     RRPPAPG"
BASE COUNT          689 a         1763 c         1735 g          845 t
ORIGIN      
        1 gtgagggcga accaggtatg gctggagcgc tcaccgattc gacagtagac gatgacctcg
       61 tctgcgggac ccagcccaag ttcgcgctgg tagagcattt cgagctcgtc acgcgacttg
      121 aaggtgccgt cctcgttcgc ggcccgcgcc cacgggacgt tcctggcacc cgggatgtgg
      181 ccaccgcgca tggcgccctc ctgcgggtag ttggccatgt gcagcagccg cccggtgaac
      241 tcgtccggag accggacgtc gaccagtggc cgcccgaggt gcgccagcac gtcatcccgg
      301 aaggcgcgga tgcgtgcgtc gtcgcggtcc accgtgggat agtcggtggt cggccggtcc
      361 ggcacatcgg tcgtcatcgg tcttccttcg gcctcccatt tggctcggcc gccgtccagc
      421 agccgcaggt cggggtgacc gaacaggctg aagacccaga tggcataggc cgcccaccag
      481 ttcgacttgt cgccgtagaa caccacggtc gagtcccggc cgatgccctt ggcactcatc
      541 agccgggcga acccggccga gtcgacgtag tcgcgcgtga caggatcgtt gagctcggtg
      601 tgccagtcga cgcgaacagc gccggggata tgcccggtgt catacagcag gatgtcctcg
      661 tcggactcga ccaccaccag ctgcgaatcg tcgcggtgct cggcgagcca ggcggtggta
      721 accagccgct cgggatgtgc gtactgcgcc agctttgggt cggaatcgtg ggggagaggc
      781 atgcaacgat cctaggcact tgtcacccag cctgccagtg cgcctatggg cgaccgcggc
      841 gacgtggcgt aggctgtgtg cgtggaaacc gacgaccgca gtcttcccgg cgagcgcgag
      901 gcgtgggatc gactccacgc cgccatcgat cgcctgacgc cggagaccgc gggcgcgacc
      961 ggctactacg ttgagggctg gaccgccaag gacgcggtcg cgcacctcgg cacctggatg
     1021 gcggagggtg cccaggtgct gcgccggatc gctgccggga ccttcctggc gggcgagctg
     1081 gacgtggacg ccgccaacgc ccgcttcctg gatgccatgc gcgacgtccc attggagacg
     1141 gttcacctgc aggccgatgc cgcccacgcc gagctgctgc gggcctacgc cgagctgccg
     1201 cgggtgacgg aggacgcggc ctactggatc cgcaaggccg gcccggagca cgtcgccgag
     1261 cacctccctc gtctcgagga gtggatcgac gagatgacgg gccggcgctg atcgaacggc
     1321 tgcctcagtc ctggagcagc ggcacgtcca ccaggtgcgc cgagaggaac cagacctgaa
     1381 gctcgtgacg gcgcaggacg tcgcccatga gcaggtcgtt gctcccgtag tcgccattct
     1441 tttcggtctt ctcgatgccg gcgcggacct tgccgatgat gatccggtgc gcctcgagca
     1501 gccgggttat ctgagccggc acctcctcag cgccgtcagg cgcgcgctcg atggtggtca
     1561 gctccgccgc gtggcgcgga tcgccgacgg cgatgccccc gagcgtctgg acccgctcac
     1621 caatgaggtc gatgagctcc agctgctctt ccgcgtgctt gtcgaagaga aggtgaagct
     1681 ggtagaacgt cggcccggcc acgttccagt gatgcttctt atagagcgcg tacaggatgg
     1741 tgctgtctgc caggatctcg ttcaggatcg tgcagctctc ggcgcgagga cgctgatcca
     1801 gctcgatggg caggtcgtgc agcttgccga acggctggac ctcgcgccca agtcgtgcca
     1861 tctgggggtt ggcgcggttg ccgtcgcgag cgccctgccg gctcgtcgtg gtgcggtccg
     1921 tggacgccgt ccggctggag cgccccccgg tgcgcgtcgc agtcgtcatc agtgatcctc
     1981 caatcggtgc atgggcttgc atattccgtg atgccgtgcc tgcacaccgc gggccatctg
     2041 gccgcgtcag atcaggagcg ccacgagcgc aaccagcgca gcgataccgc ccgacagcgc
     2101 actgatgaga agcgccttgc ggcgatccgc ggctcgtgcc tccgcatcct gcacggaggt
     2161 cgtgtcgcgg agccgcacgc cgaagttgaa gacgcccaac cagccgaagc cggcgcagaa
     2221 ccggaacgcc gcctggaggt agcccgcggc catccccacg gccgggaaga aggtcagcag
     2281 ccgccaggcc gcatcgacct ccatcgccag cagcagggcc agcagcccgg tggtggcggc
     2341 caggcccacg tgcccggtcc gccgccgcgc ggcgatctcg gccggcccga tgttgcagac
     2401 gcccggccgg tattcgggcc ccggggccgc cgtcttgagg gtcctctcca tcacgtccaa
     2461 gggtacgacg ccagttgcgc ctgtcatacg cacgagccat gtagcatcgc gcgcgtgaac
     2521 gcgccgccag acagcatccc ggccgtcctg cctcagcagc cgccgttcac catccgtgcc
     2581 cgcttgctga cgccgctgcg cgccggcggc ctccgctggg agccggatgc ggcgctgcgg
     2641 gtggacgcgc agggccgcct ggagcacatc ggtcatggat ccgcctctga tggagccttc
     2701 gacctgcggc cgtgggtggt gatgcccggc ctggttgacc tccacgccca cctcccacag
     2761 ctgcccaacg ccggtgtcgg cgccggactg cacctgctgg attggctcga gcgctacatc
     2821 tttccgctcg agcgcgggtt cgaccgcggc gtggcgaatg agctggctcc cgcggcgttc
     2881 cgcgccttcg cccacgccgg gaccacgacc gtggtcgcct acggcgccat ctggccggac
     2941 agcctggacg cctgcttcca agccgccgag acccatggca tccgcgcggt catcgggaag
     3001 gtgatgatgg accgcctctc gtacgacacc gcccggcagc cggacgaggt cctcgagcta
     3061 tccgttcgcc agtccgcgga gctgtgcgag acgtggcacg gccgtgacgc tggacggctt
     3121 tcgtacgcgt ggacgccgcg cttcgcggtg agctgctctc cgcagatgct ggcggagtcg
     3181 gcgcggcggg cggaggcggc cggcgcctac tggcagacgc acctggccga ggaccgcggc
     3241 gaagtcgagg aggtgcgccg gctcttcccg gagacaacgg actacctgga cgcctacgac
     3301 cgcgccggcg gcctgacctc gcgcgccatc ctggcgcacg ccatctacct gtccgatcgc
     3361 gaggtcgccc gccttgcgga gagcggcgcg gtggtggctc actgcccgtc ctcgaacctg
     3421 ttcctggcca gcggtgcgat gccgctggcg cgctacctcg gcgagggagt gaaggtcggc
     3481 ctgggctcgg atgtcgcggc ggggccggac gtctcgctgt ttgccgccat gcgcgccggc
     3541 gcctacaccc agaacgggct gcacgccatg ggcctggccg acacgcctgc catggccccc
     3601 ggggagtggc tgcggctcgg gaccttgggt ggcgcggagg gtcttggcct cggtgatcgc
     3661 atcgggtccc tggaggttgg caaggaagcg gacctgatcg ccgtcgacgc gtccatgacc
     3721 ctgcccctgg gcggcatgcg cgccatctcg gcggacgggc cggacgagat cctcagccgg
     3781 ctgatctatc ggtgccatcc ggacatggtc caggcggcct gggtgcgggg gcgactgctg
     3841 ccggcttagc cgccatgggc ttgcttcgtt cggctacgct acccggcgca tggaccgcat
     3901 cctccagatc ctggtcaacg ccggcgcact gtacgtggcg gtgctgatcg tgccagggct
     3961 cgacttcgag cagaccaccg acgactggtg gtggaagttc ctgctggtgg cggtcgcctt
     4021 cagcctggtg aacagctacc tgcggcccgt cctgcgcatc ctcaccttcc ccatcagcct
     4081 ggtgaccatg gggctgttcc tgctggtcat caacgcgctg atgctgctgc tcaccgggtt
     4141 gatcagcgac cagctggacc tgggcctgca ggtcgccgac ttctgggctg ccctgctggg
     4201 ctcgatcgtg atctccctgg tcggctggat cctctccatg gtggtcggcg cggcacgcat
     4261 gccggggaag gtcctctagc ctgccggacc ttccgggccc gatgtccacg gaccgcgtcc
     4321 accccctggt cgccttcgtc ttcgcggcga ccgtggccct ggccatcctc ctgacaggac
     4381 cgatgctgct cttcgccccg ccgttcgtct cgttcatgca ggcgcggcac gatgtggcgg
     4441 cgcgcctgga gaccgaccag gccaccgtgg acggcgccac cggcatcatg ctccgcgacc
     4501 tgctgctcga cggcgacttc acggtcagca ttcacgactc gggtgagccg gtcctcgacc
     4561 ccacggagcg gagccacatg cgagacgtgg gggccgtggt gcgcaccctg ctgctgatcg
     4621 acgtcgtctc gctgctgatg gtggcgctcg cggctagacg cctgcgacgc gatcctgccc
     4681 ggcgcggccg cctggtgatc atggctgcgg caggcatcgg actggcggcg ctcgtgctgg
     4741 ccgccttctt cgcactcgcc ttcgacgtgg ccttcgccgc cttccacgcg ctgttcttcg
     4801 atcccgggac gtggcagttc gggccggact cgaacctgct gcgcttcttc ccgcagccgt
     4861 tctggttcga gatcgcgctc gtggcgggtg gcagcatcgt gctcggcgcg ctgctggcct
     4921 ccgctctcgc ctggcgcgac atcgctggtt cccaccggcg ccctccggcg cccggctgac
     4981 gctcggctac actccggcca atgggtcgcg ggtcgttctc catcggcagc at
//
LOCUS       DATACA010000056         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1936280, whole genome shotgun sequence.
ACCESSION   DATACA010000056 DATACA010000000
VERSION     DATACA010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936280"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VHK28_01225"
     CDS             complement(<1..1038)
                     /locus_tag="VHK28_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX2140881.1"
                     /translation="MPHIDSGDTAWVLISTALVMLMTPGLALFYGGLVRRKNVLSTLM
                     HSFFMLALVGVTWVLWGYSLAFGPDTGLGLIGGLEWVGLEGVTGEPSSTYAPTVPHLA
                     FMAFQMMFAVITPALITGSFAERKRFKAFVLFSVAWATFVYAPVAHWVWSPDGWLYGL
                     GVLDFAGGTVVHLSSGVAALVAALMIGRRLGLVRHATEPHDVPMTVLGAGLLWFGWFG
                     FNAGSALGANGLAATAMVTTNTAAAMGALTWVTVSWLHRGTPSVVGAAIGAVAGLVAI
                     TPAAGFVSPAASILIGFGVGVVCYGASLLRLRSRADDALDVFAVHGVGGAFGALAVGV
                     FASTAVNAAGRD"
     gene            complement(1198..>2034)
                     /locus_tag="VHK28_01230"
     CDS             complement(1198..>2034)
                     /locus_tag="VHK28_01230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2140882.1"
                     /translation="LLTHEPEAARTLVARLGSQVPDLRPRFLDPDELAAAEPCLAPGY
                     AACRLETGYPVPPRSATAAWAELAERRGAAVHVGRRAVPLIEKGRATGVRLDDGTTDE
                     AGAVLLAAGPWSAEALGGRLPIRRTWGVTVQVRLGASAPRHVVEEDQVDAVNRPVAAE
                     AAAGAAAAAGDADPPSLFSIASAGGLSTLGSTFLPAEPDAARVAPLLIRRGSRFLPAL
                     ADAEVVEVRLCARPQSADGRPFIGPVEGIEGLHLCAGHGPWGISTGPPRPPWPSTPSW
                     RA"
BASE COUNT          333 a          750 c          718 g          233 t
ORIGIN      
        1 gtcgcgaccg gcggcattca ccgcggtcga ggcgaagaca ccgaccgcca gcgcgccgaa
       61 ggcgccaccc acgccgtgca ccgcgaagac gtccagggcg tcgtccgctc gcgaccggag
      121 gcgcagcagc gaggcgccgt agcacaccac gcccacgccg aagccgatga ggatcgaggc
      181 cgccggggag acgaagccgg ccgccggcgt gatggccacc agcccggcca cggcaccgat
      241 ggccgcgccc accaccgacg gcgtgccacg atgcagccag gagacggtca cccaggtcag
      301 cgcccccatg gcggccgccg tgttggtggt gaccatggcc gtggccgcca gcccgttggc
      361 gccgagggcc gagcctgcat tgaagccgaa ccagccgaac cacagcagcc cggcgcccag
      421 cacggtcatc ggtacgtcgt gcggttctgt ggcgtggcgc accagtccca gccggcgacc
      481 gatcatcagc gccgccacca gggccgccac gccgctcgag aggtggacca ccgtcccgcc
      541 ggcgaagtcg agcacgccca ggccgtagag ccagccatcc ggcgaccaga cccagtgcgc
      601 gaccggggcg tagacgaagg tcgcccacgc cacgctgaac aggacgaagg ccttgaaccg
      661 cttgcgctcg gcgaacgagc cggtgatcag cgccggggtg atcaccgcga acatcatctg
      721 gaaggccatg aaggcgaggt gcggcacggt cggcgcatac gtgctcgacg gctcgccggt
      781 gacgccctcc agcccgaccc actccagtcc cccgatcagc ccgaggccgg tatccggccc
      841 gaaagccagc gagtagcccc acagcaccca ggtgacgccc accagggcca gcatgaagaa
      901 gctgtgcatc agcgtggaga gcacgttctt gcgccgtacg agcccgccgt agaagagcgc
      961 cagcccgggc gtcatcagca tcaccagcgc ggtggagatc agcacccagg cggtgtctcc
     1021 agagtcgatg tgcggcatct gcgcgaaggc ccctctgctg catgaaggtt gtgcaggcag
     1081 cctaaggacc gatgtctccc ggccgcgacc cctcagcgac cctgccccgg gtcgctgtgt
     1141 tgtgcagtct gcacaatcac ccgattggcc ggctcgcctg cagctcgggt ggcggggcta
     1201 tgccctccag gatggagtcg atggccatgg cggccgtggc ggcccggtgg agatgcccca
     1261 cggtccgtgt ccggcgcaga ggtgcagccc ctcgatgccc tcgacgggac cgatgaacgg
     1321 gcgaccgtcg gccgactgcg ggcgtgcgca caagcggacc tccaccacct ccgcatccgc
     1381 cagggcaggc aggaagcgcg aaccacggcg gatgagcagg ggagcgacgc gcgccgcatc
     1441 gggctccgcc gggaggaagg tggatcccag cgtcgagagg ccgccggcgc tggcgatcga
     1501 gaacaggctg ggcgggtctg catcgccggc tgcggcggcg gcgccggccg ccgcctcggc
     1561 ggccactggc cggttgacgg catcgacctg gtcctcctcg acgacgtgtc gcggcgcgct
     1621 ggcacccagc ctgacctgga cggtgacgcc ccacgtgcgc cggatgggca gccgcccacc
     1681 gagtgcttcg gcggaccagg ggccggcggc cagcaggact gcgccggcct catccgtagt
     1741 cccgtcatcg agccggacac cggtcgccct gcccttctcg atcaggggca cggcccgccg
     1801 tccgacgtgg accgcggccc cgcgacgttc ggccagctct gcccaggctg ccgtggccga
     1861 ccgcggtggg accggatagc cggtctccaa ccggcaggcc gcgtacccgg gcgccagaca
     1921 cggctcggca gccgccagct cgtccggatc caggaaccgt ggccgcaggt cagggacctg
     1981 cgagcccagg cgagctacga gcgtgcgcgc cgcttccggt tcatgggtga ggag
//
LOCUS       DATACA010000057         2933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_731537, whole genome shotgun sequence.
ACCESSION   DATACA010000057 DATACA010000000
VERSION     DATACA010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731537"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VHK28_01235"
     CDS             <1..172
                     /locus_tag="VHK28_01235"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX2140883.1"
                     /translation="RATFAELRRLRDALQQATGISLPELSMGMTADAEAAVAEGATLV
                     RVGTAIFGPRHA"
     gene            204..449
                     /locus_tag="VHK28_01240"
     CDS             204..449
                     /locus_tag="VHK28_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX2140884.1"
                     /translation="MALVSTFIGMLALVLTLLILGRVLVSWTNPTGGGGLTAFLYQAT
                     EPMLAPIRRVIPPTGGIDWSPMIAILLLGVFTRLFLR"
     gene            469..744
                     /locus_tag="VHK28_01245"
     CDS             469..744
                     /locus_tag="VHK28_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX2140885.1"
                     /translation="MASLTVRVTPRASSRRVGPYRDGVLQVRVTRPPADGEANRAVQD
                     LLAAAADVSRGRVRLVAGERAREKRFEVDGLTDAELTARLRQATGAD"
     gene            784..860
                     /locus_tag="VHK28_01250"
     tRNA            784..860
                     /locus_tag="VHK28_01250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:818..820,aa:Val,seq:cac)
     gene            890..2230
                     /locus_tag="VHK28_01255"
     CDS             890..2230
                     /locus_tag="VHK28_01255"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2140886.1"
                     /translation="MPLLPASVRARLRGIRLLQPLRTRDYALLTTGAVVSLVGDGFFS
                     VALAWQVYEISNVPTAMSVVGVAWTLPLLFFLLLSGVFTDRYDRRWLMIGADLMRAAA
                     IGLLGLLSVSGGLELWHVMVLIAFVGMGDAFFNPASTAMVPDLLPDAQLPQANALQGL
                     FRPLTVRLVGPALGGFVVAVAGPGLAFMIDGASFLVSAAAIAAIRARPVRAVVAHGVR
                     QTIAEVREGLSFVASNPWLWATLVAAMFSLLVFIGPVQVLLPYLVKNRLNLGPEALGA
                     IFAVSGVGSILAAIVVGQAGLPRRRITVMYASWSVGVALFAGYGIMGSLWHAFVVGFA
                     TAALFEVGQIIWITLLQTLVPRRLLGRVSSLDWLVSTGLVPVSFALTGPVSAVLGPGP
                     TMIVAGLVGAFFMGVLLFLPGVRDPERGPRPAVLGEGADLGGALQRGIETEGRP"
     gene            2227..>2933
                     /locus_tag="VHK28_01260"
     CDS             2227..>2933
                     /locus_tag="VHK28_01260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEX2140887.1"
                     /translation="MSSTPAPGGVVAMFDRIAPVYDVMNTVMTAGIDRRWRTAALREA
                     KLRPGMRVLDVATGTGKLALAAAAAVGPRGSVTGLDASRAMLRRAARAESRRTVRREA
                     GSPISWTHADAMSMPFAAASFDAVTIGFGLRNLPGVGAALAEMARVLAPGGRVVILEV
                     GEPAKGLPRLLFRTWFRRIIPLLGRLAGRAAAYEYLPESVRRYPPPEEVAALMRQSGL
                     EEVRWRWLTSGLVTLHHG"
BASE COUNT          331 a         1060 c         1053 g          489 t
ORIGIN      
        1 gcgcgccacg tttgccgaac tgcgccggct gcgcgacgcg ctgcagcagg caaccggcat
       61 ttcgctgccc gagctgtcca tgggcatgac cgccgacgcg gaggcggccg tggccgaggg
      121 cgccaccctg gtccgggtgg ggaccgccat cttcggccct cgccacgcct gagcgagcca
      181 ccgcacgctg ctatcctccc cgcatggcac tcgtctccac gttcatcggc atgctggcgc
      241 tggtgctcac gctcctgatc ctggggcgcg tgctcgtcag ctggacgaac ccgaccggcg
      301 gtggggggct caccgccttc ctgtaccagg ccaccgaacc gatgctggcg cccatccggc
      361 gagtcattcc acccacgggg ggaatcgact ggtcgcccat gatcgccatc ctgctgctgg
      421 gcgtcttcac ccgcctcttc ctgcgctgag caggcgcgcg gccgcccgat ggccagcttg
      481 accgtgcgcg tcaccccgcg cgcatcgtcg cgccgcgtcg gtccataccg cgacggcgtg
      541 ctccaggtac gggtcacccg tccgccggcg gacggcgagg cgaaccgggc ggtgcaggac
      601 ctcctggcgg cggccgccga cgtctcacgt ggtcgggtgc ggctggtggc cggcgagcgg
      661 gcgcgagaga agcgcttcga ggtcgacggc ctgaccgacg ccgagctgac ggcgcggctg
      721 cgccaggcca cgggtgcgga ttgatcggcc tcacgccgcg ccgctatact gccctccccg
      781 tgtgggcgat tggctcagct ggttagagcg cacggttcac atccgtgagg tcactggttc
      841 gaatccagta tcgcccacca tcttcaccat ctgacatccg gaccgaccga tgccgctgct
      901 ccctgcgtcc gtccgcgcca ggctgcgcgg catccgcctc ctccaaccgc tgaggacgcg
      961 cgactacgcg ctgctgacca ccggcgccgt cgtatcactg gtgggcgacg gattcttctc
     1021 ggtggcgctg gcctggcagg tctacgagat cagcaacgtc ccgaccgcga tgtcggtcgt
     1081 gggcgtggcc tggacgctgc cgctcctctt cttcctgctg ctcagcggcg tcttcaccga
     1141 ccggtacgac cgccgctggc tgatgatcgg cgccgacctg atgcgggcgg ccgccatcgg
     1201 cctgttgggc ctgctgtcgg tcagcggcgg gctcgagctg tggcacgtga tggtgctgat
     1261 cgccttcgtg ggcatggggg atgccttctt caacccggcc tcgacggcca tggtgcccga
     1321 cctgctgccc gatgcgcagc tgccccaggc caacgcgctg caggggctct tccggcccct
     1381 caccgtgcgg ctggtgggcc cggcgctggg tggcttcgtg gtggccgtcg ccggcccggg
     1441 cctggcgttc atgatcgacg gggcgtcgtt cctggtgtcc gcggcggcca tcgccgccat
     1501 ccgcgcccgc ccggtgcggg ccgtggtcgc ccacggcgtt cggcagacga tcgccgaggt
     1561 gcgggagggg ctgtccttcg tggccagcaa cccgtggctg tgggcgacgc tggtggcggc
     1621 catgttcagc ctgctggtgt tcatcggtcc cgtccaggtc ctgctgccat acctggtcaa
     1681 gaaccgcctc aacctcgggc ccgaggcgct gggggccatc ttcgcggtca gcggggtggg
     1741 gtccatcctg gccgccatcg tcgtcggcca ggcgggactg ccgcggcggc ggatcacggt
     1801 gatgtatgcc agctggtcgg tcggcgtggc gctgttcgcc ggctacggga tcatgggcag
     1861 cctgtggcac gcattcgtgg tgggcttcgc caccgcggcc ctcttcgagg tgggccagat
     1921 catctggatc acgctgctgc agaccctggt cccgcggcgc ctgctgggcc gcgtctccag
     1981 cctggactgg ctggtctcca ccggcctggt gccggtctcc ttcgccctca ccgggccggt
     2041 atccgccgtc ctcggtccgg ggccgaccat gatcgtcgcc ggactggtgg gtgccttctt
     2101 catgggcgtc ctgctgttcc tgcccggcgt ccgcgatccc gagcgcggcc cacggccggc
     2161 ggtactcggg gagggggccg atctcggcgg cgccctccag cgcggcatcg agaccgaggg
     2221 ccggccatga gtagcacccc cgccccgggc ggggtggttg ccatgttcga ccgcatcgcg
     2281 ccggtgtacg acgtcatgaa caccgtgatg accgccggca tcgaccgacg ctggcgcacc
     2341 gccgccctcc gggaggccaa gttgcggccc gggatgcgcg tgctggacgt ggccaccggg
     2401 accggcaagc tggcgttggc ggctgcggca gcggtggggc cgcggggaag cgtgaccggc
     2461 ctggacgcct cgcgcgccat gctgcgacgc gcggcgcggg cggagtcgcg gcggaccgtg
     2521 cgtcgggaag cgggctcgcc gatcagctgg acgcacgccg acgccatgtc catgccgttc
     2581 gctgcggcca gcttcgacgc ggtcaccatc gggttcgggc tgcggaacct gcctggcgtc
     2641 ggcgcagccc tagccgagat ggcccgcgtc ctcgcgccag gcggacgggt cgtcatcctg
     2701 gaggtgggag agccagccaa gggcctgccg cggctcctct tccgtacctg gttccggcgg
     2761 atcatccccc tgctcggccg cctggccggg cgagcggccg cctacgagta cctgcccgag
     2821 agcgtccgcc gctaccctcc gcccgaggag gtcgcggccc tgatgcgcca gtcggggctg
     2881 gaggaggttc gctggcgctg gctcacgagc ggtttggtca ccctgcatca cgg
//
LOCUS       DATACA010000058         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1936908, whole genome shotgun sequence.
ACCESSION   DATACA010000058 DATACA010000000
VERSION     DATACA010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936908"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..859
                     /locus_tag="VHK28_01265"
     CDS             128..859
                     /locus_tag="VHK28_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX2140888.1"
                     /translation="MRLPYFPLHTVLFPHLPLPIHVFEERYRAMTRDVMAPDSPYAGR
                     FVVAMITEGAEVGEDSEQPARTQRVGTIVEVRRAERFGDGRWALLVVGAARALLGEVE
                     RDGPYAVIEVEPLPDEAGEAGVAVALLPAVQSALDAYMETVKRFVASAASIGAESKEI
                     TDVASSLDEVLKPIRLPDDPLAASYAVAGVLQIELTRKQQLLELPDATSRLRAELQLL
                     RREARLLADGAMPPVSTADIRYHPN"
     gene            885..2039
                     /locus_tag="VHK28_01270"
     CDS             885..2039
                     /locus_tag="VHK28_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX2140889.1"
                     /translation="MPPAHLRLIAACAVAMLVLSACGGGASPSPSAGQPSGVPSASPP
                     ASEAPGETPQPSGEESVSPEQPGETTYTVRAGDTLFSIARAWGTTVEQLQAWNAEAYP
                     SLADDPGRLQAGWVLVVSGDPNATPLPTSAPTARPATPAPSVPPPVSSGCRAGNRVAA
                     GGAQTFYTVPNAGNAVALTLDMGGRLEPGVDIMNFLIENRVCVTLFPTGAMAQTPQGQ
                     EIMAIVRAHPELFEIGNHTMHHCDLVRGGGGSPTTAPCAGVTPTADFIRKQLTDAEAI
                     LRSQTGQNPQPYWRPPFGSVNAAVIQAAAAAGYTKTFMWDVDTIDWKPVSQGGPTAQQ
                     MATKVITQARPGSNVLMHLGGYETLDALRIMVPGLRDRGFLLTSLSDLLN"
     gene            complement(2047..>3024)
                     /locus_tag="VHK28_01275"
     CDS             complement(2047..>3024)
                     /locus_tag="VHK28_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2140890.1"
                     /translation="FLTNSGTEAIEGAIKLARYHTRRPAIIAFEGAFHGRTMGALSLT
                     NSKVKQRAGFGPLLPMVYHAPFPRVRSWLEGSGGDGSAELEVLRSSIFGRLVAPSDVA
                     AIVVEPVQGEGGYFPAPRTFMEGLRALCDQHGILLVADEIQSGMGRTGRWWAMEQLGV
                     EPDIVTTAKGIASGMPIGAFIARESVWTWPAGAHGSTFAGNPICAAAGLATLQVLEDG
                     LLENAERMGALLRSGLERVAGEHGGVRDVRGLGLMVGVELASHEAANAVQQEAFERGL
                     LVLECGEATLRLCPPLIVDEELVRTAISIFAEALPAALRGLPGTAEVGG"
BASE COUNT          459 a         1123 c         1022 g          420 t
ORIGIN      
        1 ctcgcgggcc tcggggccga ccgccgcaac gagatagcgg cctgggtcgc atcggtggac
       61 agtcgcggct gaggggagcc gaacgccggc ttgcctcacc gtcggcggga tgggacgatg
      121 ccggctcatg cgtctgccgt acttcccgct ccacaccgtg ctcttcccgc acctgccgct
      181 cccgatccac gtcttcgagg agcgctaccg ggccatgacc cgcgacgtga tggctcccga
      241 ctcgccgtac gcaggccgct tcgtggtggc catgatcacc gagggcgcgg aggtcggcga
      301 ggactcggag cagcccgccc gcacgcagcg agtcggcacc atcgtcgagg tccggcgcgc
      361 cgaacgcttc ggcgacgggc gctgggcgct gctggtggtc ggcgcagcgc gcgccctgct
      421 gggcgaggtc gagcgcgacg gaccgtacgc cgtgatcgag gtcgagccgc tgcccgacga
      481 ggccggggag gctggggttg cggtcgccct gctgccggcg gtgcagtcag cgttggacgc
      541 ttacatggag accgtgaaac ggttcgtggc ctccgctgcg tccattggag cggaatcgaa
      601 ggagatcacc gacgtggcgt cctccctgga cgaagtgctc aagccgatcc ggttgccgga
      661 cgacccgctg gcggcgagct atgccgtggc cggcgtgctc cagatcgagc tgacccgcaa
      721 gcagcagctc ctcgagctgc cggacgccac gtcccgtctg cgcgccgagc tccagctcct
      781 gcgccgcgag gcccgactgc ttgccgacgg ggccatgccc ccggtcagca cggccgacat
      841 tcgataccat ccgaactgac cagcccatcc gaaccggctg accgatgcct cctgcccatc
      901 tgcgcctgat cgcggcctgc gccgtcgcca tgctcgtgct gtccgcctgt ggcggtggcg
      961 cctcgccgtc gccgagcgcc ggccagccgt cgggcgtgcc cagcgcatcg ccaccggcct
     1021 ccgaggcgcc gggcgagaca ccccagccga gcggcgagga gtccgtcagc cccgagcagc
     1081 cgggcgagac gacgtacacc gtccgcgccg gggacaccct cttctccatc gccagggcgt
     1141 ggggcaccac cgtcgaacaa ctgcaggcct ggaacgccga ggcctacccg agcctcgccg
     1201 acgacccggg caggctgcag gccggctggg tcctggtggt cagcggtgac ccgaacgcca
     1261 ctccgctgcc aaccagtgcg ccgaccgccc ggccggccac gccggctcca tcggttcccc
     1321 cgccggtgag ctcaggctgc cgggccggaa accgggtggc ggccggtggt gctcagacct
     1381 tctacacggt ccccaatgcc ggcaacgcgg tggcattgac cctggacatg ggcggccgcc
     1441 tggagcccgg ggtggacatc atgaacttcc tgatcgagaa ccgggtgtgc gtcacgctct
     1501 tcccgacagg tgccatggcc cagacgccgc agggacagga gatcatggca atcgtgcgag
     1561 cccacccgga gctgttcgag atcggcaacc acaccatgca ccactgcgac ctggtgcgcg
     1621 gcggcggtgg ttcgccgacc accgcgccct gcgccggcgt gacgccgacc gccgacttca
     1681 tccgcaagca gctgaccgat gcggaggcca tcctgcgcag ccagaccggg cagaacccgc
     1741 agccgtactg gcgtccgccc ttcggctctg tgaacgcggc ggtcatacag gccgccgccg
     1801 cggcgggtta cacgaagacc ttcatgtggg acgttgacac catcgactgg aagccggtca
     1861 gccagggtgg ccccaccgcc cagcagatgg cgaccaaggt catcacccag gcgcgacccg
     1921 gctcgaacgt cctgatgcac ctgggtggct acgaaaccct cgacgcgttg cgcatcatgg
     1981 tccccggcct gcgcgaccgc ggattcctgc tcacctcgct gagcgatctg ttgaactgag
     2041 cgcgcctcag ccgcccacct cggcggtacc cggcagccct cgcagcgcgg ccggcagcgc
     2101 ctcggcgaag atcgagatgg cggtgcgcac cagctcctcg tccacgatga ggggcgggca
     2161 caggcgcagc gtcgcctcgc cgcattcgag gaccaggagg ccgcgctcga aagcctcctg
     2221 ctgcacggcg ttggcggcct cgtggctcgc cagctcgacg ccgaccatga gcccgaggcc
     2281 gcgcacgtca cgcacgccgc cgtgctctcc cgccacgcgc tccaggccgg aacgcagcag
     2341 cgcgcccatg cgctcggcat tctcgagcag cccgtcctcc agcacctgca aagtagccag
     2401 cccggccgcc gcacagatgg ggttccccgc gaaggtcgac ccgtgggcac cggccggcca
     2461 ggtccagact gattcccgag cgatgaaggc accgatcggc atcccggagg cgattccctt
     2521 ggcggtagtc acgatgtccg gctcgacgcc gagctgttcc atcgcccacc agcggccggt
     2581 gcgtcccatc cccgactgga tctcgtcggc caccagcagg atgccgtgct ggtcgcacag
     2641 cgcccgcagg ccctccatga aggtcctcgg agccgggaag tagccaccct cgccctgcac
     2701 tggctcgacc acgatggcgg ccacgtcgct tggcgccacc aggcgtccga agatcgagga
     2761 gcgcagcacc tccagctccg cgctcccgtc gccacccgag ccctcgagcc agctgcgcac
     2821 ccgcgggaac ggcgcgtggt agaccatggg cagcaacgga ccgaagccgg cccgctgctt
     2881 gaccttcgag ttggtgaggg atagcgcccc catggttcgg ccgtggaagg cgccctcgaa
     2941 ggcgatgatg gccggccgtc tggtgtggta acgggcgagc ttgatggcgc cctcgatcgc
     3001 ctccgtgccg gagttggtga ggaa
//
LOCUS       DATACA010000059         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2097536, whole genome shotgun sequence.
ACCESSION   DATACA010000059 DATACA010000000
VERSION     DATACA010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2097536"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VHK28_01280"
     CDS             <1..595
                     /locus_tag="VHK28_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2140891.1"
                     /translation="ADRHGILLVADEVQTGMGRTGRMWATEWTDARPDIVVMAKGIAS
                     GLPLSGIMARRELLERFGPGAHGGTYGGNAVACAAALATLDVIAAEGLLENARRQGER
                     LLSGIRRAAAGHPAVAEVRGRGLMVGIELADPGTLAPRPDLAKALLEGAFERRLLLLS
                     CGTRAQVVRVIPPLVTTDTEVDAAVSAIGEALAGLPA"
     gene            complement(598..2295)
                     /locus_tag="VHK28_01285"
     CDS             complement(598..2295)
                     /locus_tag="VHK28_01285"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2140892.1"
                     /translation="MAEARGAATSPARALAWATLLLLPLLGLALVLARPELDLVWEHH
                     PSHFWLVLLAAAVNVILAFATNEAATRRGDARLILVSYAFLASAGFLGLHALATPGVL
                     LPASNAGFVVATPVGLTIAAGFAALSVSPLAGPRGHVLLRRRVALRRGLVALMVAWAV
                     VSLARLPPLNGPLPPQEAAGPLGVLAAASVPLYAWSAWRYWQLYRRRGNRLALAVAVA
                     LVLLAEAMVAVAVSRNWHLSWWEWHVLMTAAFAAIALGARAEYQRTRSLTRAFGGIYL
                     EATAARLDRWHAGAITDLVAAEGRGERAEPLLEQLRREGASEEEIALLGQAAREVGRM
                     GELFRPYLPAQLAERLGREPTLGDLGGQDREVSVLFADLANFTSFSEERPAIEVIGML
                     NAFWAEVVPVVEEVGGAIEHFAGDGMMVIFNAVGEQPDHAARAARAGLRIVERTDVLS
                     RANPGWPRFRVGINTGRAVVGNVGVEQRRSFAAIGDTSNLGARLLSGGEPGQVVISAG
                     THRALAQAGVGLAARRIGPVRVKGKREPVEAWILEGLAAAPLAGPLPPPASSPPAGG"
     gene            complement(2288..>2757)
                     /locus_tag="VHK28_01290"
     CDS             complement(2288..>2757)
                     /locus_tag="VHK28_01290"
                     /inference="COORDINATES: protein motif:HMM:NF037640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2140893.1"
                     /translation="WTDADGWAIRAVRISNGVATPYRVHAARLHLRRPDPNLLRLLGI
                     DPAAPPPSVRLEGAPRPMESPPRHLACICRLTDDGEVYRAIERGWRTVDAVKRATEAA
                     FGECQGRRCVTGLAARLDLTPADPGGSITPRPPLVPVPASVLAAFAGDHGPGG"
BASE COUNT          376 a         1042 c         1020 g          319 t
ORIGIN      
        1 ggccgaccgc cacggcatcc tgctcgtcgc cgacgaggtg cagaccggca tgggtcgcac
       61 cggccgcatg tgggccaccg agtggaccga cgcccggccg gacatcgtgg tcatggccaa
      121 gggcatcgcc tccggcctgc ccctgtccgg gatcatggcc cgccgcgagc tgctggagcg
      181 cttcgggccg ggggcccacg gtgggaccta cggtggcaac gcggtcgcct gcgccgcggc
      241 cctggccacc ctggacgtga tcgccgccga ggggctgctg gagaacgcgc ggcgccaggg
      301 cgagcggctg ctgtccggga tccggcgggc cgcggccggc catccggccg tggcagaggt
      361 gcgcggtcgc gggctgatgg tcgggattga gctggccgac cccggcacgc tcgctccacg
      421 ccccgacctg gccaaagcgc tgctggaggg cgcgttcgag cggcggctgc tgctgctgtc
      481 ctgcggcacc cgtgcacagg tggtccgcgt catcccgccg ctggtgacga ccgataccga
      541 ggtggatgcg gccgtgtcgg ccatcggcga ggcgctggcc ggcctgcccg cctgaggtca
      601 gccgcctgcc ggtggactcg aggccggggg cggaagtggg cccgcgagtg gggccgcggc
      661 cagaccttcg aggatccagg cctcgaccgg ctcccgcttg cccttgaccc gcaccggacc
      721 gatgcgcctg gcggccagtc ccacgccggc ctgcgccagg gcacggtgcg tcccggcgct
      781 gatcaccacc tggccaggct ctccgcccga cagcaggcgc gcacccaggt tgctggtgtc
      841 gccgatggcc gcgaagctgc gccgctgctc aacgccgacg ttgcccacca ccgcgcgccc
      901 cgtgttgatc ccgacccgga agcgcggcca gccggggttg gcgcgtgaca ggacgtcggt
      961 gcgctccacg atgcgcagcc cggcccgcgc ggcacgcgcg gcgtggtcgg gctgctcgcc
     1021 cacggcattg aagatgacca tcatcccgtc gccggcgaag tgctcgatcg cgccgccgac
     1081 ctcctccacc actggcacca cctcggccca gaaggcgttg agcatgccga tcacctcgat
     1141 ggccgggcgc tcctcgctga agctggtgaa gttggccagg tcggcgaaca ggacgctgac
     1201 ctcgcggtcc tgcccgccga ggtcgccgag cgtcggctcg cggccgagcc gctcggccag
     1261 ctgcgcgggc aggtacggcc ggaacagctc gcccatgcgc cctacctctc gcgccgcctg
     1321 gcccagcagt gcgatctcct cctcgctggc cccctcgcgg cgcagctgct ccaggagggg
     1381 ctcggcgcgc tcgccacggc cctcggccgc caccaggtcg gtgatggcgc ccgcgtgcca
     1441 gcggtcgagc cgagcggcgg tcgcctccag gtagatgccg ccgaacgcgc gcgtcagcga
     1501 gcgggtgcgc tggtactccg cgcgagcccc cagcgcgatg gccgcgaatg cagccgtcat
     1561 cagcacgtgc cactcccacc agctgaggtg ccagttgcgg ctgaccgcca ccgcgaccat
     1621 cgcctcggcc agcaggacca gcgccacggc caccgccagc gccagccggt tcccacggcg
     1681 gcggtacagc tgccagtagc gccacgccga ccaggcgtac agcgggaccg atgccgccgc
     1741 cagcaccccc agcggaccgg ccgcctcctg cggcggcagc gggccgttca acggcggcag
     1801 acgggccagc gagacgacag cccaggcgac catcagcgcc accaggccgc ggcgcagcgc
     1861 cacccgccgg cgcagcagta cgtggccgcg cggcccggcc agcggactca ccgacagggc
     1921 cgcaaacccg gcggcgatgg tcaggccgac cggggtggcc accacgaagc cggcgttgga
     1981 ggccggcagc aggacgccgg gagtggccag cgcgtgcagg cccaggaagc cggcactggc
     2041 caggaatgcg taggagacca ggatcagccg ggcgtcgccg cggcgcgtgg ccgcctcgtt
     2101 ggtggcgaag gccaggatca cgttgacggc ggctgccagc agcaccagcc agaagtggct
     2161 gggatggtgc tcccacacca ggtccagctc cggccgggcc aggaccagcg ccagccccag
     2221 caggggcagc aggaggagcg tggcccatgc gagggcacgg gccggcgatg tcgcggcgcc
     2281 acgcgcctca gccaccggga ccgtggtcgc cggcgaaggc agccaggacc gatgccggga
     2341 ccggcaccag cggtggccgc ggggtgatgg atccgcccgg atcggcaggc gtgaggtcca
     2401 ggcgtgcggc caggccggtc acgcagcgcc ggccctggca ctccccgaat gccgcctccg
     2461 tagcgcgctt gaccgcatcg accgtgcgcc agccgcgctc gatggcgcgg tacacctcac
     2521 cgtcgtcggt caggcggcag atgcacgcca ggtggcgcgg tggcgattcc atgggtcgcg
     2581 gcgctccctc caaccggacc gatggtggcg gcgcggccgg gtcgatcccc agcagacgga
     2641 ggaggttcgg gtccggccgc cgaaggtgca ggcgtgcggc atgcacgcgg tacggcgtcg
     2701 ccaccccgtt gctgatgcgc accgcccgga tggcccagcc gtccgcatcg gtccatg
//
LOCUS       DATACA010000060         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1856807, whole genome shotgun sequence.
ACCESSION   DATACA010000060 DATACA010000000
VERSION     DATACA010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856807"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1139)
                     /locus_tag="VHK28_01295"
     CDS             complement(372..1139)
                     /locus_tag="VHK28_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140894.1"
                     /translation="MAAVLAGCSPAAAQPVVPAVATVQPTSAPTLAPTPRPTPVPTLA
                     PTPVPTPSPTPKPEPQPFALNLFEEGDFVPQHTFDWCVGASIQMAWNLVRSDRRSSYE
                     DQQAIWEMARDRSSNSFRGANPRGWASVLTELGLGQHEFTTIPDYADALRTAARALRE
                     TNRPVGLVMWRGRHAWVMSGFTSIGDPAIHDDFTVTGIYVLDPLYPHGSGTWGPSPVP
                     NTLMTPEQLAKQFVIREPRRWTLGMQAGYPLVLPIGD"
     gene            1621..2964
                     /locus_tag="VHK28_01300"
     CDS             1621..2964
                     /locus_tag="VHK28_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX2140895.1"
                     /translation="MSQATQRRFVLPESELPTHYYNIAADLPEAPPPPLHPGTREPIG
                     PEMLAPLFPMELIKQEVSAERLIPIPEPVRHAYRVYRPSPLIRALDLEASLNTPARIY
                     YKYEGVSPAGSHKPNTALAQAFYNRAEGVTRLVTETGAGQWGSALAYAGAQFGIETKV
                     FMVRVSYEQKPYRRSFIQAMGASVVPSPSTETNAGRAILDSDPDSSGSLGIAISEAVE
                     EAATRDDTKYSLGSVLNHVLLHQTVIGQEAMRQMEMAGDVPDVVIGCAGGGSNFAGIA
                     FPFLRDRMNGASRTRFLAVEPASCPSLTRGEYRYDFGDTAGTTPLLKMHTLGNHFRPA
                     PIHAGGLRYHGMAPMVSHLYELGFLEARAYAQNACFEAALRFARTEGIVPAPESSHAI
                     RAAIDEAEEARQLGEERVILFNLSGHGYFDMGAYDAYLAGALQDVEFAISEAVAV"
     gene            complement(2986..>3680)
                     /locus_tag="VHK28_01305"
     CDS             complement(2986..>3680)
                     /locus_tag="VHK28_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140896.1"
                     /translation="PVQAEPIPSSPSRPGRKLVPVAVLLLLLVAAGFAGQRWVASLSD
                     RETDSPYAVVPVEGTPTANPAQIRTASPRPSDVVNPTPTPIAIPEPTAAATAEPRSET
                     PVPETPAPATPAPEPPPETPAAVAVLEPQDSVTRFYSLVSAGRFDEAYGLWSDRMRAS
                     YPRQVNLDDRFAQTASVTVHRLALVANDGRRAVVAIDFTETYDTGSWRRFDGSWELVN
                     VGGGWLLDVPHF"
BASE COUNT          576 a         1203 c         1345 g          556 t
ORIGIN      
        1 tcgcgctcgc cggcctccac cggttcctgg tcgatgccgt gggtctcgcc gtctgtgcga
       61 ctggcgttgc gatcggtcgt catgcgaggt ggaccgatgc gcatgcgccg tgccatgcga
      121 ctgtgcggca ctggtgcagc agcaatacca cgttccgcct ctgggtgtgt cgcttcttcc
      181 ggaaatgcgt accggtgaag cattgcgtcg aggatgccgc ccttgcaggc tcgattcgtc
      241 acggatcgtt ggcgtcaggc ggtgctggta ccgcaaatca ggaagatcct gtggcaatcg
      301 ggtctaatcg ctgagaggct gcgccaccgg taccgcatcg gcagcttcgc gattgcgaac
      361 gaccgcgagc gctagtcgcc gatcggcagc accaacgggt agcccgcctg catgccgagc
      421 gtccagcgac gcggctcgcg aatcacgaat tgctttgcca gttgttcggg ggtcatgagc
      481 gtgttgggga ccggcgacgg accccacgtc ccgctgccgt gcgggtagag cgggtcgagg
      541 acatagatgc cggtcaccgt gaagtcgtca tggatggccg ggtcgccaat ggacgtgaag
      601 ccgctcatca cccaggcgtg gcgcccgcgc cacatcacga gtcccacggg gcggttcgtc
      661 tcccgcaggg cgcgggcggc ggtgcgcagt gcgtcggcgt agtcggggat ggtcgtgaac
      721 tcgtgctgcc cgagccccag ctcggtcagg accgacgccc agccgcgcgg gtttgcccca
      781 cggaacgagt tggacgatcg gtcgcgggcc atctcccaga tcgcctgctg atcctcgtag
      841 gagctgcgcc gatccgatcg gacgaggttc caggccatct ggatgctggc gcccacgcac
      901 cagtcgaagg tgtgctgcgg gacgaagtca ccttcctcga acaggttgag ggcgaacggc
      961 tgcggctccg gcttcggtgt cggcgacggg gtgggaaccg gcgttggggc gagcgtagga
     1021 accggggtgg ggcgcggcgt cggcgccagg gtcggtgcgc tggtcggctg aacggtcgcg
     1081 acggcaggga cgacgggctg agcggcggcc gggctgcagc cagccagaac ggccgccacc
     1141 agaagggcgg cccatggtgc acggattcgt ctgcgggtcg tgctcatctg gtcctgcgaa
     1201 gtgtagtgcg aaagggtcgt gatctcccta tcggtgccgg ttactcaggg cgcgggagcg
     1261 cggcccgcat tgacacgcgg caggcccgag gcgtagggtg cgcggcacat ctgaagatgg
     1321 cgacgatcgg gaggagtacc cggtcaggcg gatccacagg gagccgggag cactgagacc
     1381 ccggcggtcg cgcgaccggc gaatgggccc gggagccccg gaggcgaacg cggacgcatc
     1441 ggtccaccag tagccgacgg cggagaggca ccgataccaa agccgaggcc atcgcctgcg
     1501 tgagcgagcc gtggccgaac gagcccgggt gagaacaccg gggaagaaag gtggcaccac
     1561 gagaagaccc cctcgtcctt ttggacggag gggtcgtgtt gttctcggag gtgtacaccg
     1621 atgtcccagg cgacgcagcg gcggttcgtg ctcccggagt cggagctgcc cacgcactac
     1681 tacaacatcg cggccgacct gccggaggct ccgccgcccc cactgcaccc cggcacccgt
     1741 gaacccatcg gtcccgagat gctggcgccg ctattcccga tggagttgat caagcaggag
     1801 gtcagcgccg agcgcctgat cccaataccg gagccggtgc ggcacgcgta ccgcgtttac
     1861 cggccgtcac cgctcatccg tgcgctggac ctggaggcca gcctcaacac gccggcgcgc
     1921 atctactaca agtacgaggg cgtctcgccg gcgggctccc acaagccgaa caccgccctg
     1981 gcccaggcgt tctacaaccg cgccgagggc gtgaccaggc tggtcaccga gaccggcgcc
     2041 ggccagtggg gaagcgccct ggcctacgcg ggtgcccagt tcggcatcga gaccaaggtg
     2101 ttcatggtgc gcgtcagcta cgagcagaag ccgtaccgac gcagcttcat ccaggccatg
     2161 ggagccagcg tggttccttc gccgtcgacc gagacgaacg cggggcgggc gatcctggac
     2221 tcggaccctg actcatcggg gagcctgggc atcgccatca gcgaggcggt cgaggaggcg
     2281 gccacgcgcg acgacacgaa gtactcgctg ggcagcgtgc tgaaccacgt gctgctgcac
     2341 cagacggtca tcggccagga ggccatgcgg cagatggaga tggccggcga cgtgcctgac
     2401 gtggtcatcg gctgcgccgg aggagggtcg aacttcgcgg gcatcgcctt ccccttcctg
     2461 cgcgaccgga tgaacggcgc cagccggacg cggttcctgg cggtcgagcc ggcgtcgtgt
     2521 cccagcctga cgagggggga gtaccggtac gacttcggcg acaccgccgg gacgacgccg
     2581 ctgctcaaga tgcacaccct gggcaaccac ttccgcccgg cgccgatcca cgccggtggc
     2641 cttcgctacc acggcatggc acccatggtc tcgcacctgt acgagctcgg cttcctggag
     2701 gctcgggcct acgcgcagaa cgcctgcttc gaggcggccc tgcgcttcgc tcgcaccgag
     2761 ggcatcgtcc cggcgccgga atcgtcgcac gccatccggg ccgcgatcga cgaggcggag
     2821 gaggcgcggc agctgggcga ggagcgggtc atattgttca acctgtcggg gcacgggtac
     2881 ttcgacatgg gggcatacga cgcctacctg gccggagcgc tccaggacgt ggagttcgcg
     2941 atttcggagg cggtggccgt gtgacgacct gccgatgggg cgcactcaaa agtgcggcac
     3001 gtccaggagc cagccaccgc ccacgttgac gagctcccag gagccgtcga agcgccgcca
     3061 tgacccggtg tcgtaggtct cggtgaagtc gatggcgacg acggcccgcc ggccgtcgtt
     3121 cgccaccagc gccaggcggt gcacggtgac cgatgcggtc tgcgcgaacc ggtcgtccag
     3181 gttcacctgc cgcgggtagc tggcccgcat ccggtcgctc cacaggccgt acgcctcgtc
     3241 aaagcggccg gcgctcacca gcgagtagaa gcgggtgacg ctgtcctgcg gctccaagac
     3301 ggccaccgcg gccggcgttt cgggtggcgg ctcgggagcc ggggtggccg gcgccggagt
     3361 ctccgggacc ggcgtttcgc tgcgcggctc tgcggtggcc gccgccgtcg gctcggggat
     3421 ggcgatcggt gtgggcgtgg ggttcaccac gtcgctgggc cgcgggctgg cggtccgaat
     3481 ctgtgccggg ttcgcggttg gcgtcccctc tacggggact acggcgtagg gggagtcggt
     3541 ctcgcggtcg gataggctgg cgacccatcg ctgcccagcg aacccggccg cgaccagcag
     3601 caggagcagg acggcaacag gcaccagctt tcgacccggg cgcgacggcg atgacgggat
     3661 cggttcggcc tggaccggtt
//
LOCUS       DATACA010000061         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_571623, whole genome shotgun sequence.
ACCESSION   DATACA010000061 DATACA010000000
VERSION     DATACA010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571623"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VHK28_01310"
     CDS             <1..1327
                     /locus_tag="VHK28_01310"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX2140897.1"
                     /translation="EVLGWGGYWAWDPVENAALLPWLTATAFIHSSMVAERRGTLRAW
                     THALVLATFVLTLVGTFLTRSGVVQSVHSFTASPIGPWFLVAILLALAGGLALLVWRL
                     PDLGDSRPPGAVVSRESAFLFNNILFLGVTFAVLFGTLYPTVREAISGERVSLGAPWF
                     NGVNAPIFVALLFLMGVGPALPWGGASWSTVRERFVAPLLVGGMATAVAWLLGVNDPA
                     PLAAIALSGFVLVVMGHEVLRGARARSRQRGEAAPVATWRLLTRNRRRYGGYLVHAGI
                     CVMAVAVAISATLASDVTASLRPGESLQLGNYTVTHRDLVSEPLASDPRVLETRAELD
                     LAGPQSGPMSPALRDYPSSATLIATPAVRTAAGEDLYVTLLAFEPSDGSVTLHVFVNP
                     LVAWIWIGGGIVAGGAVFAAWPDRRRRAEVAQRTAAVEGVPVPAAGGER"
     gene            1324..>1518
                     /locus_tag="VHK28_01315"
     CDS             1324..>1518
                     /locus_tag="VHK28_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEX2140898.1"
                     /translation="MSRRAPRWRWLAIPVLVLPLAWVLGAGLARPVPRVGDPAPAFEL
                     TTIDGRTISSSDLEGRAYVLN"
BASE COUNT          161 a          553 c          557 g          247 t
ORIGIN      
        1 cgaggtgctg ggctggggcg gctactgggc ctgggacccg gtggagaacg ccgccctgct
       61 gccgtggctg acggccacag ccttcatcca ctcgtccatg gtcgccgagc ggcgagggac
      121 gctgcgcgcc tggacccacg cattggtgct ggcgaccttc gtcctgaccc tggtgggcac
      181 cttcctgacc cgctcgggcg tggtgcagag cgtccactcc ttcaccgcct cgcccatcgg
      241 gccctggttc ctggtggcca tcctgctggc cctggccggc gggttggcgc tgctcgtctg
      301 gcggctgccg gacctggggg acagccggcc gcccggcgcc gtggtgagca gggagagcgc
      361 gttcctgttc aacaacatcc tcttcctggg ggtcaccttc gcggtgctgt tcgggacgct
      421 gtatcccacc gtccgcgagg caatctccgg cgagcgggtc agccttgggg ccccgtggtt
      481 caacggcgtc aacgccccga tcttcgtggc gctcctgttc ctgatgggcg tcggaccggc
      541 cctgccctgg ggtggcgcgt cgtggtcaac cgttcgcgag cgcttcgtgg ccccgctgtt
      601 ggtcggcggg atggcgaccg cggtcgcctg gctgctgggc gtcaacgacc ccgcgcccct
      661 ggccgccatc gctctgtccg ggttcgtgct ggtggtgatg ggccacgagg tgctgcgcgg
      721 cgcgcgcgcc aggagtcggc agcgcggcga ggcggcgccg gttgccacct ggcgcctgct
      781 gacccgcaac cggcggcgct acggcggtta cctggtgcac gcgggcatct gcgtcatggc
      841 cgtggcggtc gccatctcgg ccaccctggc ctcggacgtc acggccagcc tgcgaccagg
      901 cgagagcctg cagctgggca actacacggt cacccaccgg gacctggtca gcgagccgct
      961 ggcgtccgat ccgcgcgtgc tcgagacccg cgccgagctg gatttggccg gcccccagtc
     1021 cggaccgatg tctcccgccc tgcgtgacta ccccagctcc gccacgctga tcgccacccc
     1081 ggcggtgcgc acggctgccg gcgaggacct gtacgtcacg cttctcgcct tcgagccctc
     1141 agacggcagc gtgaccctgc acgtcttcgt caacccgctg gtggcctgga tctggatcgg
     1201 tggcgggatc gtggccggcg gcgcggtctt cgccgcatgg ccggacaggc ggcgccgtgc
     1261 ggaagtggcg cagcggacgg ccgccgttga aggcgtcccg gtccccgcgg cgggagggga
     1321 gcgatgagcc gccgggcgcc gcgctggcgc tggctggcga tcccggtgct ggtgctgccg
     1381 ctggcctggg tgctgggcgc cggcctggcc cggccggtgc cgcgcgtcgg cgacccggcg
     1441 ccagccttcg agctgaccac catcgacggc cgcacgatca gctccagcga cctggaggga
     1501 cgggcctacg tcctcaac
//
LOCUS       DATACA010000062         9125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2419404, whole genome shotgun sequence.
ACCESSION   DATACA010000062 DATACA010000000
VERSION     DATACA010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9125
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419404"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="fusA"
                     /locus_tag="VHK28_01320"
     CDS             <1..652
                     /gene="fusA"
                     /locus_tag="VHK28_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535588.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX2140899.1"
                     /translation="RPQVSYREAIRKPVRAEGRFVRQTGGKGQYGHAIVEFEPLERGV
                     GFEFESKVVGGSVPREFWKAIEEGIRDAFAGGIVAGYPVIDVKARLVDGSYHEVDSSE
                     MAFKIAGSMAAKDGVAKADPAILEPVMRVDVTTPDEFMGDVIGNLNSRRGHIDGIDEH
                     GTSRVIHAQVPLAEMFGYATELRSMTQGRATYSMEFSRYAEVPTNLANELIAKSKS"
     gene            684..1889
                     /gene="tuf"
                     /locus_tag="VHK28_01325"
     CDS             684..1889
                     /gene="tuf"
                     /locus_tag="VHK28_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005385492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX2140900.1"
                     /translation="MAKQKFDRSKPHVNVGTIGHVDHGKTTLTAAITKFLSLSGGADF
                     RAFDSIDNAPEERQRGITIAIAHVEYQTANRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVEVPSLVVYLNKVDMVDDEMLVDLVEMEVRELLSK
                     YEFPGDDIPVIRGSALKALESASTDPESEDYASIKELMDAVDSYIPTPVRDVDKPMLM
                     PIEDVFGIKGRGTVATGRIERGIVHTGDTLELVGIHQEKPRQLVVTGVEMFKKTLDEG
                     QAGDNVGLLLRGIERDEIERGQVLAKPGSVKPGTKFKAEVLVLSKEEGGRHTPFYSGY
                     RPQFYLRTTDVTGAIALPAGVEMIMPGDNVNIDVELITPIAIEQGQRFAIREGGHTVG
                     AGVITEVLG"
     gene            1895..2203
                     /gene="rpsJ"
                     /locus_tag="VHK28_01330"
     CDS             1895..2203
                     /gene="rpsJ"
                     /locus_tag="VHK28_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX2140901.1"
                     /translation="MAKQRIRIRLKAYDHKLLDQSAEQIVETAQRTGAAIAGPVPLPT
                     RIEKYTVIRGPFVHKDGREQFEIRTHKRLIDIIDPSTKTVDALMRLSLAAGVDIEIKM
                     "
     gene            2272..2895
                     /gene="rplC"
                     /locus_tag="VHK28_01335"
     CDS             2272..2895
                     /gene="rplC"
                     /locus_tag="VHK28_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX2140902.1"
                     /translation="MPAGLIGRKLGMTRIFGDDGTVVPVSVIEVTPNSITRLRTPEQD
                     GYAALQVGAGTARRQTKPVAGQFKHLDKDQPRPATVREFRVDSLDGYEVGQPLDVSLF
                     EAGELVDVIGTSKGQGFAGTIKRHNFKRGPKTHGSDNYRAPGSIGAGTSPGRVWKGTR
                     MGGHMGDERVTVKKLKVVRVDSERNLLLVKGAVPGARNGLLLVQKAK"
     gene            2898..3521
                     /gene="rplD"
                     /locus_tag="VHK28_01340"
     CDS             2898..3521
                     /gene="rplD"
                     /locus_tag="VHK28_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX2140903.1"
                     /translation="MAETTILNANGSEAGKLELAEALFGAKINTALIHQAAVRQLAGR
                     RQGTSDTLTRAEVSGGGAKPWRQKGTGRARQGSRRAPHWAGGGVTFGPHPRSYEQKMN
                     AAMRRAALKGVLSAKAADGALRVVAGFEMPEIKTRALAERLDAWKAEGKVLVVLPGRD
                     HVVERSCRNLSHVRVILADSLNVVDLLEADTIVFTQEALSRAEEVYA"
     gene            3518..3814
                     /gene="rplW"
                     /locus_tag="VHK28_01345"
     CDS             3518..3814
                     /gene="rplW"
                     /locus_tag="VHK28_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX2140904.1"
                     /translation="MSATTVHDVLIRPVISEKSVAETERNNYTFAVAKDANKYQIKAA
                     VEAEFKVNVLDVRVMSVKPKQKRRGRRQMGTVPGWRKAVVTIAAGQKIELFEAV"
     gene            3816..4649
                     /gene="rplB"
                     /locus_tag="VHK28_01350"
     CDS             3816..4649
                     /gene="rplB"
                     /locus_tag="VHK28_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX2140905.1"
                     /translation="MPLRKYRPTSPGVRQMTRPTFEEVTTDTPHKPLTERLLSRGGRN
                     SQGKLSVRHQGAGHKRLYRVIDWKRDKAGVPARIATVEYDPNRSARIALLHYADGEKR
                     YILLPHGLGVGDTVVSGPDVEARTGNAMPLGRMPLGTQVHNVELTAGRGGQIVRSAGS
                     SAQLLAREGTMVTLRLPSGEVRRVHATCTATVGQVSNLDHENQKLGKAGRARHMGRRP
                     TVRGSVMTPRDHPHGGGEGKSPTGMPPKTPWGKPAMGLRTRSKKKGSSKMIVRRRYGR
                     G"
     gene            4659..4943
                     /gene="rpsS"
                     /locus_tag="VHK28_01355"
     CDS             4659..4943
                     /gene="rpsS"
                     /locus_tag="VHK28_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX2140906.1"
                     /translation="MSRSVKKGPFVDARLLGRIDDMNKRNERKVLKTWSRASVIFPQM
                     IGHTIAVHDGRRHVPVFVTENMVGHRLGEFAPTRHYRGHGKATERSSALK"
     gene            4954..5295
                     /gene="rplV"
                     /locus_tag="VHK28_01360"
     CDS             4954..5295
                     /gene="rplV"
                     /locus_tag="VHK28_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX2140907.1"
                     /translation="MRVTATAKYIRISPRKVRLVTDLIKGKPVEEAAAILRFLPNAAA
                     RDVAKLLRSATANAENNYNLSADELTVVSATADDGPTLKRGRPRAQGRYFAILKRTSH
                     ITIAVADREEA"
     gene            5295..6365
                     /gene="rpsC"
                     /locus_tag="VHK28_01365"
     CDS             5295..6365
                     /gene="rpsC"
                     /locus_tag="VHK28_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX2140908.1"
                     /translation="MGHKVHPYGFRIGINKPWLAKWYADRDYASLLQEDMRIRQLVAK
                     QLSNASVSQVEIERGINHVTVTVHSAKPGIVIGKGGANVEALRTNVGKLTDKKVKLEI
                     KEILQPELDGVLVAQNVAGQLERRIAFRKAIKQTVQRTIKAGAKGVRIQVSGRLGGSE
                     MSRTEWDKEGRIPLGTLRADISYGMVHALTTAGRIGVKAWIYRGEQLGERPGSARTEP
                     REARRPARGRNERPSAGGSTRPAPARAATATEAPTGTGPETAQPEMDVAPVAKAAQDE
                     TAQAADSTQTDTAQAEAAAEAPRAEVAESEASAEPEESAAPEPTAEPEATAEAEPAAA
                     PEEPAGKSPAPKAKAERKGKGA"
     gene            6368..6796
                     /gene="rplP"
                     /locus_tag="VHK28_01370"
     CDS             6368..6796
                     /gene="rplP"
                     /locus_tag="VHK28_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX2140909.1"
                     /translation="MLMPKRVKHRKVQRGRRAGLAKGGTSVSFGDYGLMAEEVCWLTS
                     RQIEAARRAMTHHIKRGGRIWIRVFPDKPVTKKPAEVRMGSGKGAPDHWVAVVKPGRV
                     MFEMSGVPEPIAREAMRLASHKLPISTKFVVKEGLEHGHQ"
     gene            6783..7007
                     /gene="rpmC"
                     /locus_tag="VHK28_01375"
     CDS             6783..7007
                     /gene="rpmC"
                     /locus_tag="VHK28_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013805286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX2140910.1"
                     /translation="MDISEVRRLSDPDLEKALLDAKQELWKIRFDLATRQEKNYSRLP
                     ATRKQIARILTVMTERQRDATASADAEKAS"
     gene            7007..7276
                     /gene="rpsQ"
                     /locus_tag="VHK28_01380"
     CDS             7007..7276
                     /gene="rpsQ"
                     /locus_tag="VHK28_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX2140911.1"
                     /translation="MQGKRRTKVGRVVSDKMDKTVVVSVERLRRHPIYKRVVRLSSKF
                     KAHDAENSAHVGDTVRIEESKPLSREKRWRVVEVVARGSGEELVD"
     gene            7277..7645
                     /gene="rplN"
                     /locus_tag="VHK28_01385"
     CDS             7277..7645
                     /gene="rplN"
                     /locus_tag="VHK28_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX2140912.1"
                     /translation="MIRQYTRLRVADNSGARVLQTITVIGRSRSDTAEVGDTIVASVK
                     QALPNSGVKKGDVVRAVIVRTAKEYGRPDGSHIRFDENAAVLINAQGSPRGTRIFGPV
                     ARELRERNYMKIVSLAPEVL"
     gene            7648..7974
                     /gene="rplX"
                     /locus_tag="VHK28_01390"
     CDS             7648..7974
                     /gene="rplX"
                     /locus_tag="VHK28_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2140913.1"
                     /translation="MNVRKGDTVLVLAGKDRGKRGTVERVERTKRGLGVVIPGINMAR
                     RHQRPRTRTQQAGILDIPVPIHVSNVQVVCPRCGKATRIGHQRLENGRTVRVCKHCGE
                     QLEGAR"
     gene            8076..8609
                     /gene="rplE"
                     /locus_tag="VHK28_01395"
     CDS             8076..8609
                     /gene="rplE"
                     /locus_tag="VHK28_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2140914.1"
                     /translation="MRQRYHDEVVPAMQREFNYANPMQVPRLEKVVVNIGLGEAIQNA
                     KALDAAVGDLAIITGQKPIVNRAKRSIAQFRLRTGMPIGAKVTLRGQRMYDFLERTMR
                     MALPRIRDFRGIATRSFDGRGNFSLGLREQLVYPEIDYDKIDRLRGLEISIVTTAKTD
                     EEGRMLLQLLGMPFQST"
     gene            8633..8818
                     /locus_tag="VHK28_01400"
     CDS             8633..8818
                     /locus_tag="VHK28_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2140915.1"
                     /translation="MAKKSMIAKSQRKPRFEVRQHNRCSICGRPRGYMRRFALCRICF
                     RERALEGQLPGVTKSSW"
     gene            8920..>9125
                     /gene="rpsH"
                     /locus_tag="VHK28_01405"
     CDS             8920..>9125
                     /gene="rpsH"
                     /locus_tag="VHK28_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2140916.1"
                     /translation="MTTTDPIADLLTRIRNASAARHTEVTLPSSRVKREIARILAEEG
                     FVGGVSTSQDGVQEMLTVQLKYVEG"
BASE COUNT         1712 a         3016 c         3226 g         1171 t
ORIGIN      
        1 tcggccgcag gtcagctacc gcgaggccat tcgcaagccg gtgcgcgccg agggccgctt
       61 cgtgcggcag accggcggca agggccagta cggccacgcc atcgtggagt tcgagccgct
      121 ggagcgcggt gtgggcttcg agttcgagtc gaaggtcgtg ggtggcagcg tgccacgcga
      181 gttctggaag gcgatcgagg aggggatccg agacgccttc gccggtggca tcgtggccgg
      241 ctacccggtc atcgacgtca aggcccggtt ggttgacggc tcgtaccacg aggtcgactc
      301 gtcggagatg gccttcaaga tcgcgggcag catggccgcc aaggacgggg tggccaaggc
      361 cgatcccgca atcctggagc cggtgatgcg cgtggacgtc accacccccg acgagttcat
      421 gggcgacgtc atcggcaacc tgaacagccg ccgcggccac atcgacggca tcgacgagca
      481 cggcacgtcg cgcgtcatcc acgcccaggt gccgctggcc gagatgttcg ggtacgccac
      541 cgagctgcgc agcatgacgc agggtcgtgc cacctattcg atggagttct cccgctacgc
      601 ggaagttccc accaacctcg ccaacgagct catcgcgaag tcaaagagct agagcgcaca
      661 agaggagcga cgaaggacaa accatggcaa agcagaagtt cgatcgcagc aagccgcacg
      721 tgaacgtcgg caccatcggt cacgtcgacc atggaaagac gacgctgacc gcggcgatca
      781 ccaagttctt gtcgctgagc ggcggggcag acttccgcgc cttcgactcg atcgacaacg
      841 ctcccgagga gcggcagcgc ggcatcacca tcgcgatcgc ccacgtcgag taccagacgg
      901 cgaatcgcca ctacgcgcac gtcgactgcc cggggcacgc ggactacatc aagaacatga
      961 tcactggcgc ggcccagatg gacggcgcca tcctggtggt gtccgccgcc gacggcccca
     1021 tgccccagac ccgcgagcac atcctgctgg cgcgccaggt ggaggtgccc tcgctcgtcg
     1081 tctacctcaa caaggtggac atggtcgacg acgagatgct ggtcgacctc gtggagatgg
     1141 aggtgcgcga gctcctcagc aagtacgagt tccccggcga cgacatcccg gtcatccgcg
     1201 gctccgccct caaggcgctg gagtcggcga gcaccgaccc cgagtcggag gactacgcgt
     1261 cgatcaagga gctgatggac gcggtcgaca gctacatccc caccccggtg cgcgacgtcg
     1321 acaagccgat gctgatgccg atcgaggacg tgttcggcat caagggccgc ggaacggtgg
     1381 ccaccggccg catcgagcgc ggcatcgtgc acaccggcga cacgctggag ctggtgggca
     1441 tccaccagga gaagccgcgc cagctggtgg tgaccggcgt agagatgttc aagaagaccc
     1501 tggacgaggg ccaggcgggc gacaacgtcg gcctgctgct gcggggcatc gagcgtgacg
     1561 agatcgagcg gggccaggtg ctggccaagc ccggcagcgt caagccgggc accaagttca
     1621 aggccgaggt gctggtcctg tccaaggagg agggcgggcg ccacacgccg ttctactcgg
     1681 gataccggcc gcagttctac ctgcgcacga ccgatgtcac cggcgcgatc gcgctcccgg
     1741 caggcgtcga gatgatcatg ccgggtgaca acgtcaacat cgacgtcgag ctgatcacgc
     1801 cgatcgccat tgagcagggg cagcgcttcg cgattcgcga gggcggccac accgtcggcg
     1861 ccggcgtcat caccgaggtc ctcggctagc ccccatggcc aagcagcgca tccgcatccg
     1921 cctcaaggcg tacgaccaca agctgctcga ccagtcggcc gagcagatcg tggagacggc
     1981 gcagcgcacc ggggccgcca tcgcgggccc ggtgccactg ccgacccgga tcgagaagta
     2041 caccgtcatc cgcggaccgt tcgtccacaa ggatggccgg gagcagttcg agatccggac
     2101 tcacaagcgg ctgatcgaca tcatcgatcc gtcgaccaag acggtggatg cgctgatgcg
     2161 gctgagcctg gccgccggcg tggatatcga aatcaagatg tgacgcggac cggccctcgc
     2221 gggccggctc gcaccgcaga ccccagcacc gagaaggaag tagacgagac agtgccggca
     2281 ggattgatcg ggcggaagct cggaatgacg cgcatcttcg gcgacgacgg aacggtcgtc
     2341 ccggtgagcg tcatcgaggt cacgcccaac agcatcacgc ggctccgcac gccggagcag
     2401 gacggctatg ccgccctgca ggtcggcgcc ggtaccgcgc gccgtcagac caagcccgtg
     2461 gccggccagt tcaagcacct ggacaaggac cagccgcggc cggcaaccgt tcgggagttc
     2521 cgcgtcgact ccctggatgg ttacgaggtc gggcagccgc tcgacgtctc gctgttcgag
     2581 gccggggagc tggtcgacgt catcggcacc agcaagggcc aggggttcgc cgggaccatc
     2641 aagcgccaca acttcaagcg cgggccgaag acgcacggat cggacaatta ccgggcaccg
     2701 ggctccatcg gtgccgggac ctcgccgggc cgtgtctgga agggcacgcg catgggcggc
     2761 cacatggggg atgagcgggt gaccgtcaag aagctcaagg tcgtcagggt ggactccgag
     2821 cgcaacctgc tcctggtcaa gggagcggtg ccgggtgcgc gcaacggcct gctgctggtg
     2881 cagaaggcga agtgaccatg gccgaaacga cgatcctcaa cgcgaacggc agcgaggccg
     2941 ggaagctgga gctggccgag gcgctgttcg gcgccaagat caacaccgcg ctgatccacc
     3001 aggccgctgt gcggcagctg gccggtcgcc gccagggcac ctccgacacg ctgacccggg
     3061 ccgaggtgtc cggtggcggt gccaagccat ggcgccagaa ggggaccggt cgcgcccgcc
     3121 agggcagccg tcgggctcca cactgggccg gcggcggcgt gaccttcggg ccgcatccgc
     3181 gcagctacga gcagaagatg aacgccgcca tgcgccgcgc cgccctcaag ggcgtgctga
     3241 gcgccaaggc cgcggacggt gcgctacggg tggtcgctgg cttcgagatg ccggagatca
     3301 agacccgcgc cctcgccgag cggctggatg cctggaaggc cgagggcaag gtgctggtgg
     3361 tgctgccggg gcgcgaccac gtcgtggagc gctcctgccg caacctctcg cacgtgcggg
     3421 tcatcctggc cgacagcctg aacgtggtcg acctgctgga ggcggacacc atcgtcttca
     3481 cccaggaggc gctgtcgcgc gccgaggagg tgtacgcatg agcgccacca ccgtgcacga
     3541 cgtgctcatc cggccggtca tcagcgagaa gagcgtggcc gagacggagc gcaacaacta
     3601 caccttcgcg gtggcgaagg atgcgaacaa gtaccagatc aaggcggcgg tcgaggccga
     3661 gttcaaggtg aacgtcctcg acgtccgcgt gatgtccgtg aagcccaagc agaagcgccg
     3721 cgggcgccgg cagatgggca ccgtgcccgg ctggcgcaag gcggtcgtga cgatcgcggc
     3781 gggccagaag atcgagctct tcgaggccgt ttgacatgcc acttcgcaag taccgaccaa
     3841 ccagtcccgg cgtgcggcag atgacgcgcc cgaccttcga ggaggtcacg accgatacgc
     3901 cgcacaagcc gctcaccgag cgcctgctgt ccaggggtgg ccgcaactcg cagggcaagc
     3961 tcagcgtccg ccaccagggc gccgggcaca agcgcctgta ccgcgtcatc gactggaagc
     4021 gggacaaggc cggcgtgccg gcccggatcg cgactgtcga gtacgacccg aaccgctcgg
     4081 cgcggatcgc gctgctgcac tacgcggacg gcgagaagcg ctacatcctg ctgccgcatg
     4141 gccttggcgt gggtgacacc gtggtgtccg gcccggacgt cgaggcgcgt accgggaacg
     4201 ccatgccgct ggggcgcatg ccccttggca cccaggtcca caacgtggaa ctgaccgccg
     4261 gccgcggcgg gcagatcgtg cgctcggccg gcagcagcgc gcagctgctg gcgcgggagg
     4321 ggaccatggt caccctgcgc cttccctccg gggaggttcg ccgggtgcac gccacctgca
     4381 cggcgacggt cggccaggtg agcaacctgg accacgagaa ccagaagctg ggcaaggccg
     4441 gccgggcccg tcacatgggc cggcgcccga cggtgcgcgg ctcggtgatg accccccgcg
     4501 accacccgca cggcggtggc gagggcaagt cgcccaccgg aatgccgccc aagacgccat
     4561 ggggcaagcc ggccatgggc ctgcgcacca ggtcaaagaa gaagggcagc agcaagatga
     4621 tcgtccgccg tcggtacgga cggggttagg aggcttcgat gagtcgttcc gtcaagaagg
     4681 gaccgttcgt cgacgcgcgc ctgctcggcc gcatcgacga catgaacaag cgcaacgagc
     4741 gcaaggtgct caagacctgg tcgcgcgcca gcgtcatctt cccgcagatg atcggccaca
     4801 ccatcgcggt gcatgacggc cgccggcacg tgccggtgtt cgtcaccgag aacatggtcg
     4861 gtcaccgtct gggcgagttc gccccgacgc gtcactaccg tggtcacggg aaggccaccg
     4921 agcgctcctc ggcgctgaag taggagctgg ttcgtgcgcg tgacggcaac cgccaagtac
     4981 atccgaatca gcccgcgcaa ggtgcgcctg gtcaccgatt tgatcaaggg caagccggtc
     5041 gaggaggccg cggccatcct gcgcttcctg ccgaacgcgg cggcgcgcga cgtggccaag
     5101 ctgctgcgca gcgcaaccgc caacgcggag aacaactaca acctatcggc cgacgagctc
     5161 accgtggtga gcgcaacggc ggacgacggg ccgaccctaa agcgtggacg tccgcgcgcc
     5221 cagggtcgct atttcgcaat tctgaagagg accagccaca tcaccatcgc tgtggctgac
     5281 cgtgaggagg cctgatggga cataaggttc atccatacgg cttccggatc ggcatcaaca
     5341 agccgtggct ggccaagtgg tacgcggatc gcgactacgc gagcctgctg caggaggaca
     5401 tgcggatccg gcagctggtc gcgaagcagc tatccaacgc cagcgtcagc caggtcgaga
     5461 tcgagcgtgg gatcaaccac gtcaccgtga ccgtccactc cgccaagccg gggatcgtga
     5521 tcggcaaggg cggggcgaac gtcgaggcgc tgcgcacgaa cgtcggcaag ctcaccgata
     5581 agaaggtcaa gctcgagatc aaggagatcc tgcagccaga gctggacggg gtcctggtgg
     5641 cgcagaacgt cgccggccag ctcgagcggc gcatcgcctt ccgcaaggcg atcaagcaga
     5701 ccgtccagcg caccatcaag gccggagcca agggcgtgcg catccaggtc tccgggcggc
     5761 tcggcggctc cgagatgagc cgaaccgagt gggacaagga ggggcgcatc ccgttgggca
     5821 ccctccgcgc cgacatcagc tacggcatgg tgcacgcgct gacgaccgcg ggacgcatcg
     5881 gcgtcaaggc ctggatctac cgcggcgagc agctcggtga gcgcccgggc tcggcccgca
     5941 ccgagccgcg tgaggctcgg cggccggcgc gcggtcgcaa cgagcgtccc agcgcgggcg
     6001 gttccacccg gccggctccc gcgcgggcag caaccgccac cgaggcgccg accggaaccg
     6061 ggcccgagac cgcgcagccg gagatggacg tagcgccggt cgccaaggcg gcgcaggacg
     6121 aaacggcgca ggctgccgat agcacacaga ccgataccgc ccaggccgag gccgcggcgg
     6181 aggcacctcg agcagaggtc gccgagtcgg aggcatcggc cgagccggag gaatcggctg
     6241 cgccggagcc aacggccgag ccggaggcaa ctgccgaggc cgagcctgcg gcagcgcccg
     6301 aggagccggc cggcaagtcc ccggcgccca aggccaaggc cgagcgcaaa ggcaaggggg
     6361 cctgagcatg ctgatgccaa agcgcgtcaa gcaccgcaag gtgcagcgcg gacgacgagc
     6421 gggactggcc aagggtggca cgagcgtcag cttcggagac tacggcctga tggccgagga
     6481 ggtctgctgg ctgaccagcc gccagatcga ggctgctcgc cgcgcgatga cgcaccatat
     6541 caagcgcggt ggccggatct ggatccgtgt cttcccggac aagccggtaa ccaagaagcc
     6601 cgccgaggtg cggatgggct cgggcaaggg agcgccggac cactgggtcg cggtggtcaa
     6661 gcccgggcgc gtgatgttcg agatgtccgg tgtgccggag ccgatcgccc gcgaggcgat
     6721 gcgcctggcc agccacaagc tgccgatctc gacgaagttc gtcgtgaagg agggcctgga
     6781 acatggacat cagtgaggta cggaggctct ccgaccctga cctggagaag gcgctgctgg
     6841 acgcgaagca ggagctgtgg aagatccgct tcgacctggc cacccggcag gaaaagaact
     6901 acagcaggtt gccggcgacg cgcaagcaga tcgcccgcat cctgacggtg atgacggagc
     6961 gccagcgaga cgctacggcg tccgctgacg cggagaaggc cagctgatgc agggcaagag
     7021 acgaaccaag gtcggacgcg tggtgtccga caagatggac aagaccgtgg tcgtgagcgt
     7081 tgagcggctg cgccggcacc cgatctacaa gcgggtggtt cggctgtcga gcaagttcaa
     7141 ggcgcacgat gcggagaact cggcgcatgt gggcgacacg gtgcgcatcg aggagagcaa
     7201 gccgctgtcc cgcgagaagc gctggcgcgt ggtcgaggtc gtggctcgcg gctccggcga
     7261 ggagctggtg gactagatga tccgccagta cacccgcctg cgggttgcgg acaacagcgg
     7321 cgcacgagtg ctgcagacca tcaccgtcat cggccgctcg cgctccgaca cggccgaggt
     7381 gggcgacacc atcgtggcca gcgtgaagca ggccctgccc aactcgggcg tgaagaaggg
     7441 cgacgtggtg cgcgccgtga tcgtgcgcac cgccaaggag tacgggcggc cggacggcag
     7501 ccacatccgc ttcgacgaga acgcggcggt gctgatcaac gcccagggca gcccgcgcgg
     7561 cacgcgcatc ttcgggccgg tggcccgtga gctccgggag cgcaactaca tgaagatcgt
     7621 ttcgcttgcc ccggaggtgc tgtagcgatg aacgtacgaa agggcgacac cgtgctggtg
     7681 ctcgccggca aggatcgcgg caagcggggc accgtggagc gcgtggagcg caccaagcgg
     7741 ggcctgggcg tcgtgatccc cggaatcaac atggctcgcc gccaccagcg gccgcggacc
     7801 cggacccagc aggccggcat cctggacatc ccggtgccca tccacgtcag caacgtgcag
     7861 gtcgtctgcc cgcgctgcgg caaggcgacc cgcatcggtc accagcggct ggagaacggc
     7921 cggacggtgc gcgtctgcaa gcactgcggc gagcagctgg agggtgccag gtgagcgcgg
     7981 acaccgcgac caagccgaag gccaaggcca aggcgcccgc gcgccccgcg gcgtccggga
     8041 aggacggggc cttctcgcct ccgacctacc acgggctgcg gcagcggtac cacgacgagg
     8101 tggtgcccgc catgcagcgc gaattcaact acgccaatcc gatgcaggtt ccgcgactgg
     8161 agaaggtcgt ggtcaacatc ggtctggggg aggccatcca gaacgcgaag gcgctggatg
     8221 ccgccgtcgg cgacctggcc atcatcaccg gccagaagcc gatcgtgaac cgcgccaagc
     8281 ggtcgatcgc ccagttccgc ctgcggacgg gaatgccgat cggggccaag gtgaccctgc
     8341 gcggccagcg gatgtacgac ttcctggagc gcaccatgcg catggcactg ccgcgcattc
     8401 gcgacttccg gggcatcgcc acccgctcgt tcgacgggcg cggcaacttc agcctggggc
     8461 tgcgcgagca gctcgtctac ccggagatcg actacgacaa gatcgaccgc ctccgggggc
     8521 tggagatcag catcgtgacc acggcaaaga ccgatgagga ggggcgcatg ctgctccagc
     8581 tcctcggcat gccgttccag tccacctgac ggaaggagcc tgaggcaaca ccgtggccaa
     8641 gaagtccatg atcgcgaagt cgcagcgcaa gccgcgcttc gaggtccggc agcacaaccg
     8701 gtgctcgatc tgcgggcgcc cgcgtggcta catgcggcgc ttcgcgctct gccggatctg
     8761 cttccgggag cgggccctgg agggccagct gccgggcgtc accaagtcga gctggtagct
     8821 cgtcaacatc ggccgtcgtg agacggcccc atcagcggca ggcgccaacc acgccgcacg
     8881 cgtggcgcga accaccgccg aggagacgaa tcgcaaacca tgaccaccac tgatccgatc
     8941 gccgacctgc tgacgcgcat ccgcaatgcg agcgcggctc ggcacaccga ggtgacgctg
     9001 ccctcgtcca gggtgaagcg cgagatcgcg cgcatcctgg cggaggaggg cttcgtcggc
     9061 ggcgtgtcga ccagccagga cggcgtgcag gagatgctca ccgtgcagct gaagtacgtt
     9121 gaggg
//
LOCUS       DATACA010000063         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_170408, whole genome shotgun sequence.
ACCESSION   DATACA010000063 DATACA010000000
VERSION     DATACA010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170408"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VHK28_01410"
     CDS             complement(<1..494)
                     /locus_tag="VHK28_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX2140917.1"
                     /translation="MVTACGENGPLPFQPTPEPTPVQPREVAVLAVDRGSDAPVPGAE
                     VSVDGETSVTGQDGSAVVTALPGDRVEITAPGYDPGSETVPDEGGLEVDLRPNVVAGT
                     VTDAAGEPVEGVRVFVDGSELYVRTDADGAYALPGVPESGTLIYKRAGYRLAELPIDE
                     QMTK"
     gene            complement(545..1279)
                     /gene="rpe"
                     /locus_tag="VHK28_01415"
     CDS             complement(545..1279)
                     /gene="rpe"
                     /locus_tag="VHK28_01415"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2140918.1"
                     /translation="MSLRRSPQPRPRPPHISASVLNADFAQLGAEVRRAVSGGVDSIH
                     LDVMDGHFVDNITMGPVVVEAVRSASSVPYHAHLMISEPLRYAGRFAEAGSDVVVFHL
                     EADDDPAAVIRAIRDAGRSPGLAINPETPAAAATPWLEQVDMLLVMTVRPGWGGQEFM
                     HEVLPKVAELRAEADRRGLDLPIGVDGGVGLDTIGPAYAAGGDVLVTGTALYRNPGDL
                     SEVVDGLKAAAREGLQPIPTPERSRS"
     gene            complement(1279..2448)
                     /gene="rlmN"
                     /locus_tag="VHK28_01420"
     CDS             complement(1279..2448)
                     /gene="rlmN"
                     /locus_tag="VHK28_01420"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX2140919.1"
                     /translation="MTELQLAGPAPAISEVDPAALAAWLEARGEPAYRAEQVLSGVHR
                     REASGFEDLTDLPAALRAELARAFRFSTIAASHVLVADNGLTAKAVHQLHDGQRIESV
                     LMRYPARGGAAERTTICISSQAGCAVNCPFCATGQAGFGRQLTPGEIVDQVLHWHRAP
                     WQALGSDWRPGSRTGHYNIVFMGMGEPLNNADRVFEAVRLLNDPQRLGIGARHITVST
                     SGVVPGIDRMIAELPQVNLAISLHAADDAVRDDLVPLNRKWPLEQLIAAGRRFSDATG
                     RRVSLEYVMIDGVNDGLPLAEALVALARGWRSHVNLIPLNPTPGSRWSGTPPVRVRAF
                     ADVLRAGGVAVTVRDTRGREIEAACGQLHAQLAGKALPRAGASTGARSVEPELAR"
     gene            complement(2441..3181)
                     /locus_tag="VHK28_01425"
     CDS             complement(2441..3181)
                     /locus_tag="VHK28_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX2140920.1"
                     /translation="MGFFTYVLLVMLPSIAITAWAAWRVRSTYAKWSKVDSGINMSAF
                     DFARRLLDRQGLTDVRIEPVPGQLTDHYDPRGKVLRVSSAVSGHPELGSYADGQIGLG
                     VPGSDRLSVAAAAVIAHEVGHALQDREGDPWMAVRQAIVPAVQFGSSIAPWLIIAGLI
                     FNMLSLAWVGLIAFGAAVVFTFVTLPVEIGASGKALAFVSGLGVSGERETGARDVLKA
                     AAWTYVAAALTALMTFLYYLMLIAGRRD"
     gene            3367..3921
                     /locus_tag="VHK28_01430"
     CDS             3367..3921
                     /locus_tag="VHK28_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2140921.1"
                     /translation="MSTHRSDRAIREARAAVDRLAGRDRPGPGRYLLVGLMGALGGAV
                     AAFLLDPQRGRTRRIQLGDRTAALARDVGREVERRRRAVTSTVAGKAEAARHAGEEGP
                     LPNDEALAAKVETELFRDPDVPKGGININVEQGRVVLRGEVPDDGMRKKLEKSAAAIP
                     GVWDVENLLHLPGEPAPTYDRVTS"
     gene            complement(3918..>4271)
                     /locus_tag="VHK28_01435"
     CDS             complement(3918..>4271)
                     /locus_tag="VHK28_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX2140922.1"
                     /translation="ASIIRPFTRADGWIDWRRPAEELDRRVRALQPWPGTWTTLDGRR
                     LHVRAAHPVAGVDDVPIGVLLPGDEPRVACGQGALALDVVQPEGRPAMPGDAWRRGLA
                     RDHVLLGAVAPVAGR"
BASE COUNT          637 a         1558 c         1512 g          564 t
ORIGIN      
        1 tccttggtca tctgctcgtc gataggcagc tcggccaggc ggtagccggc ccgcttgtag
       61 atcagggtcc cgctctcggg gaccccgggt agcgcatagg caccgtccgc atcggtcctg
      121 acgtacagct cgctcccgtc gacgaagacg cgcacgccct ccaccggctc tccggccgcg
      181 tcggtgaccg tgccggcgac cacgttgggc cgcaggtcga cctccagccc gccctcgtcc
      241 gggacggtct cggagcccgg gtcgtagccg ggtgccgtga tttcgacccg gtcgccgggt
      301 aacgccgtga ccaccgcgct gccgtcctgg ccggtgaccg acgtctcgcc atccacgctc
      361 acctcggcgc cgggcaccgg cgcgtccgag ccgcgatcga cggccagcac ggcgacctcg
      421 cgcggctgga ccggggtcgg ctccggcgtc ggctggaagg gaagcggccc gttctcgccg
      481 caggcggtga ccaccagcga gacgaccagg aggatgagga gaggtcgcga ggcgcgggga
      541 cgggtcagga tcggctcctt tcgggggtgg gaatcggctg caggccttcg cgcgccgcag
      601 ccttgaggcc gtcaacgacc tcgctcaggt caccggggtt acggtacagg gcggtgccgg
      661 tgaccagcac gtcaccaccg gccgcgtagg cgggaccgat ggtatcgagc cccaccccgc
      721 cgtccacccc gatcggcagg tcgagcccgc ggcggtccgc ctccgcgcga agctcggcga
      781 ccttgggaag cacctcgtgc atgaactcct ggccgcccca gccgggacgg acggtcatga
      841 ccaacagcat gtccacctgc tccagccagg gcgtggcggc cgcggccggc gtctccgggt
      901 tgatggccag ccctggcgag cggcccgcgt cgcgaatggc gcggatgacc gcggccgggt
      961 cgtcatcggc ctccaggtgg aagacgacga cgtcgctgcc cgcctcggcg aagcgcccgg
     1021 cgtagcgcaa cggctcgctg atcatgaggt gggcgtggta cggcacgctt gacgcggacc
     1081 gcaccgcctc gaccaccacc ggccccatgg tgatgttgtc cacgaagtgg ccgtccatga
     1141 catccaggtg gatggagtcc acgccgccgg acaccgcccg gcgcacctcg gcccccagct
     1201 gtgcgaagtc ggcgttcagc acgctggcgc tgatgtgcgg cggccgcggc ctgggttgcg
     1261 ggctgcgtcg caagctcatc acctggcgag ctccggctcg acggatcgcg ccccggtgga
     1321 cgcccccgcc cgcggcaggg ccttgccggc cagctgggcg tgcagctgtc cgcaggcggc
     1381 ctcgatctcg cggccgcggg tgtcgcgcac ggtgaccgcc acgccgccgg cgcgcagcac
     1441 gtcggcgaag gcgcggaccc ggaccggggg cgtgccgctc caccgcgagc cgggggtggg
     1501 attgagcggg atcaggttca cgtggctgcg ccagccacgg gctagcgcca ccagcgcctc
     1561 ggccaacggc aggccgtcat tgacgccgtc gatcatcacg tactccaggc tcacccgccg
     1621 tccagtggcg tcggagaagc gccgcccagc cgcgatcagc tgctccagcg gccatttgcg
     1681 attcagcggg accaggtcgt cgcgcaccgc gtcgtccgcg gcgtggaggc tgatggccag
     1741 gttgacctgc ggcagctcgg cgatcatgcg gtcgatgccc ggcaccacgc ccgacgtgct
     1801 caccgtgatg tgccgcgccc cgatgcccag ccgctgcggg tcgttcagca ggcgcaccgc
     1861 ctcgaacacg cgatccgcat tgttcagcgg ttcacccatg cccatgaaca cgatgttgta
     1921 gtggccggtg cgggaacccg gccgccagtc ggaccccaac gcctgccacg gtgcgcgatg
     1981 ccagtgcagc acctggtcca cgatctcccc cggggtcagc tgacgcccga agcccgcctg
     2041 cccggtggcg cagaaggggc agttgaccgc acagccggcc tggctcgaga tgcagatggt
     2101 ggttcgctcg gcggcgcctc cccgcgccgg gtagcgcatc aggacggact cgatgcgctg
     2161 gccgtcgtgc agttggtgga cggccttggc ggtcagccca ttgtcggcca cgagcacgtg
     2221 gctggcggcg atggtcgaga aacggaaagc gcgcgccagc tcagcgcgca gcgcggcggg
     2281 caggtcggtc aggtcctcga acccgctcgc ctcccgccga tgaacgcctg acagcacctg
     2341 ttcagcccga tacgcaggct cgccgcgggc ctcgagccac gcggcgagtg ctgccgggtc
     2401 gacctcgctg atcgccggcg ctgggccagc gagctgcagc tcagtcacgg cgtccggcga
     2461 tcagcatcag gtagtacagg aaggtcatca gggcggtgag cgccgcggcc acgtaggtcc
     2521 acgccgccgc cttgaggacg tcgcgtgcgc cggtctcgcg ctcgcctgac actcccaggc
     2581 cggagacgaa ggccagtgcc ttgccggatg cgccgatctc caccggcagc gtgacgaagg
     2641 tgaacaccac cgccgcaccg aaggcgatca aaccgaccca ggccagcgag agcatgttga
     2701 agatgagccc ggcgatgatc agccacggcg cgatgctcga gccgaactgc acggccggca
     2761 cgatcgcctg ccgcacggcc atccacgggt cgccctcgcg gtcctgcaga gcgtgaccca
     2821 cctcgtgggc gatcacggcg gctgccgcca ccgaaagccg gtcactgccc gggacgccca
     2881 gcccgatctg gccgtcggcg tacgacccaa gctcgggatg cccggacacg gcgctgctca
     2941 cgcgcagcac cttgccgcgc gggtcgtagt gatcggtcaa ctggccggga acgggctcga
     3001 tgcgcacatc ggtcaggccc tgccggtcca gcagccgccg tgcgaagtcg aaggctgaca
     3061 tgttgatgcc agagtcgacc ttcgaccact tggcgtaggt agagcgcacg cgccacgcag
     3121 cccacgctgt gatggcgatg cttggcagca tcaccagcag cacgtacgtg aagaatccca
     3181 tgttcgttgc cacctcctgg aagccagccg agtataccgt ccgcttggtg aaggttcggt
     3241 caaggcccga tggagaggcg tccacatgct tggctcccgg tcggccttcg gcacgccgcg
     3301 tgcgggaggg aggttggcag gaaccacggc acgcagcacg ccgcagaaca cgaggaagga
     3361 agaccgatga gcacccaccg ttccgacaga gcaatccgcg aggcccgcgc cgccgtcgat
     3421 cggctcgccg gccgcgatcg tcccggcccc ggccgctacc tgctggtcgg gctgatgggc
     3481 gcgctcggcg gcgcggtcgc ggctttcctc ctggatccac agcggggacg cacccgccgc
     3541 atccagctcg gcgaccgcac cgccgccctg gcgcgtgacg tgggtcgcga ggtcgagcgc
     3601 cgccggcgcg cggtgacttc caccgtggcc ggcaaggccg aggccgcccg ccacgccggc
     3661 gaggagggcc cgctgcccaa cgacgaggcg ctggcggcca aggtggagac cgagctcttc
     3721 cgggacccgg acgtcccgaa gggcggcatc aacatcaacg tggagcaggg tcgcgtggtg
     3781 ctgcgcggcg aggtgccgga cgacgggatg cgcaagaagc tggagaagtc agccgccgcg
     3841 attcccggcg tgtgggacgt cgagaacctc ctccacctcc ccggggagcc cgccccgacc
     3901 tacgaccgcg tcaccagcta gcgtcctgcc accggcgcca ccgcgccgag caggacgtgg
     3961 tcgcgagcca gcccccggcg ccacgcgtcg ccaggcatgg cgggcctgcc ctccggctgc
     4021 acaacgtcga gggccagcgc cccctggccg caggcgaccc gcggctcatc cccggggagc
     4081 agcaccccga tcggcacatc gtccacgccg gccacggggt gtgccgcgcg cacgtggaga
     4141 cgcctgccgt ccagggttgt ccaggtaccc ggccagggct gaagggcgcg cacgcgacgg
     4201 tcgagctcct cggccgggcg ccgccagtcg atccagccgt cggcgcgggt gaaggggcgg
     4261 atgatgctcg c
//
LOCUS       DATACA010000064         5147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_973732, whole genome shotgun sequence.
ACCESSION   DATACA010000064 DATACA010000000
VERSION     DATACA010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973732"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VHK28_01440"
     CDS             <1..611
                     /locus_tag="VHK28_01440"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX2140923.1"
                     /translation="SDAAVAAELREQVLDELNVKSLQVLDDESEMVERTLYPLLPVVG
                     PRHGAAVRAIMSGARSGEWQLREDGRVEVGGVVLAPDEFQLTARARPGHEVAEEGDLL
                     VALDTELTPELEAEGLAREVAHRIQNLRKSAGYAIDDRIAVAIGGDAAVVEQLAAHDE
                     WLAGEVLAVELRLAPDAVLEDPDAGEELEAGEGRLRLAVRRG"
     gene            644..1153
                     /gene="lspA"
                     /locus_tag="VHK28_01445"
     CDS             644..1153
                     /gene="lspA"
                     /locus_tag="VHK28_01445"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX2140924.1"
                     /translation="MSSEPPRWTSPGDLAALGLTAVVIVAADQATKALVVAGMRVGER
                     IEVIGDLVMLWHVQNTGGAFSLLPGSLPLFYAVTVVALVMIAWFHRSMLGRGLWLHVV
                     LGMVLGGTIGNLIDRVRQGYVTDFVSMGFGETRFPTYNVADASLVVGILVLVAYLTFA
                     DSRPREVRA"
     gene            1150..2142
                     /locus_tag="VHK28_01450"
     CDS             1150..2142
                     /locus_tag="VHK28_01450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX2140925.1"
                     /translation="MTRPAAAGEEMRVVEGVAEAGRFDLAVSAVGGISRAHAQRLIGD
                     GRALVDGRRARSSDRLRGGERVTVELSAPADQTLKAESIPLSIVYEDEAILIVDKPPG
                     LVVHPSAGHASGTLVNALLGQARERGQSLGSVAGVGRPGIVHRLDKDTSGLLVVARTD
                     AAQASLMRQFGDRSVGKEYLALVRGEAPAPRGRVEAPVGRDPRDRQRMAVVAGGRESV
                     TEYEVVATGGGYSLLALHPRTGRTHQIRAHLAYLRLPIAGDLRYGGGIGPGGLERQFL
                     HAAVLELHRPLDGRRLRAWSGLPPDLAASLAAAGVDLTRAGSRLPVGSGAEVVG"
     gene            2145..2768
                     /gene="gmk"
                     /locus_tag="VHK28_01455"
     CDS             2145..2768
                     /gene="gmk"
                     /locus_tag="VHK28_01455"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX2140926.1"
                     /translation="MDEVQVKRLAFPNPMLVVVSGPPGVGKSTIVAELARGHPQVVPI
                     VTVTTRQQRPDETDGVHYHFVSDADFQALIAADGLLEHANNHGYWYGTPVNQVRGILA
                     AGRDAILTISPEGARNVRAQVPGALLIFVMPPSLDDLDERMRARGSETVESLERRRRD
                     AERWMAEKDDYDHVVVNETGHPEWAAERIWEIIQAEARREPPRQVRI"
     gene            2783..5146
                     /gene="priA"
                     /locus_tag="VHK28_01460"
     CDS             2783..5146
                     /gene="priA"
                     /locus_tag="VHK28_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX2140927.1"
                     /translation="MPAEPGRNVRVAVDAIADRPERTFSYLLPPELDAAEPGSLVLVP
                     YGRRLALGYLMPGEPEAVDGAELKAVEAVLSEPMLTPDLLHLAEAIAEHYRAPVGTTL
                     AAMLPPGLESRLERRWEVLDPSALPPGLASGVASDADVSHAALLRHAPRRGREAWVER
                     LRRSGAIRARWTLRRPDVQARTVRVLRPLSGGVLPARRAPLQRAMLEALGGEERTLPE
                     LAEALGVDLASLLGPARRLAAIGAAELSAREVARDPLAHRAPATSVREGLAAEQVAAL
                     HQIEGLPPGGELLLEGVAAAGKTEVYLAAVAAVLKAGEGAVLLVPELSMVPQLADRVA
                     GVVGDRLAVLHSGLSAGERHDEWWRILRGEARVVVGTRTAVFAPVQRLGLIVVDEAHD
                     GGYKSDRTPRYDARWSAARRAARTGARLVLGTATPDVVTIARVRGGMVERARLRERRV
                     GRAPEVEVVDLRAELASGNRSVFSAALAAALGELRAGSEQAILLMNRRGASTFILCRD
                     CGESLRCPDCDLPFVFHATDELLRCHHCGRSARPPERCPRCDSRRIRYFGAGTQRVEA
                     ELRSRFPGLQVARLDSDALAARRGFEAIYDDFRDGQVDVLVGTQLAAKGLDLPSVTLA
                     AVIAADVTLNLPDYLAPERTFQLLAQVAGRAGRGPMPGRVIIQTYAPDHYAVKAAARM
                     DVDGFADEELTRRRLLGYPPASVLARLLLADPDRERAEARAEAAASAVRVPGVEVHGP
                     LAAYVARSAGRWRFQVILRAERGADRRAALERVPAGVGIDVDPESLL"
BASE COUNT          636 a         1772 c         2002 g          737 t
ORIGIN      
        1 gcagcgacgc ggcggtggcc gctgagctgc gcgagcaggt cctcgatgag ctcaacgtga
       61 agtcgctcca ggtgctcgac gacgagtcgg agatggtcga gcgcaccctc tacccgctgc
      121 tcccggtggt cggtccgcgg cacggcgccg ccgtgcgggc catcatgtcc ggcgctcgct
      181 ccggcgagtg gcagctccgc gaggacggcc gggtggaggt cggtggggtg gtgctggcgc
      241 cggacgagtt ccagctgacg gcgcgggccc gtcccggcca cgaggtggcc gaggagggcg
      301 acctgctggt ggcgctggac acagagctga cccccgagct ggaggcggag gggctggccc
      361 gcgaggtggc gcatcgcatc cagaacctcc gcaagtcggc gggctacgcc atcgacgacc
      421 ggatcgcggt ggccatcggc ggcgatgcgg cagtcgtgga gcagctggct gcccatgacg
      481 aatggttggc cggcgaggtg ctggccgtcg agctgcggct ggcgccggat gccgtgctcg
      541 aggatcccga cgccggggag gagctggagg ccggcgaggg ccggctgcgg ctggccgtgc
      601 gccgcgggtg agggagcccg atccgatcgc gtaccatccg gaaatgagca gcgagccgcc
      661 gcggtggacc agccccggcg acctcgcagc gctgggactc accgcggtgg tcatcgtggc
      721 ggccgaccag gcgaccaagg cgctggtggt ggccggcatg agggtggggg agcggatcga
      781 ggtcattggc gacctggtca tgctgtggca cgtgcagaac actggcgggg cgttcagcct
      841 cctgccgggc agcctgccgc tcttctacgc ggtcacggtg gtggccctgg tcatgatcgc
      901 ctggttccat cggtccatgc tggggcgcgg gctttggctg cacgtcgtgc tgggcatggt
      961 cctgggaggc acgatcggca acctgatcga tcgcgtgcgc cagggctacg tcaccgactt
     1021 cgtgtccatg ggcttcggcg agacgcgctt cccgacctac aacgtggctg acgcgtcgct
     1081 ggtggtcggc atcctggtgc tggtggccta cctgaccttc gccgacagcc gcccccgcga
     1141 ggtgcgggcg tgacgcgacc ggccgcggcc ggcgaggaga tgcgggtggt ggagggcgtg
     1201 gccgaggcgg gccgcttcga cctggcggtg agcgccgttg gtggcatctc gagggcccac
     1261 gcccagcgcc tgatcggcga cggccgagcg ctggtggacg gacgtcgggc gcgctccagt
     1321 gaccgcctgc gcggcggcga gcgcgtcacg gtcgagctca gcgcccctgc cgaccagacg
     1381 ctcaaggcgg agtccatccc gctctccatc gtctacgagg acgaggcgat cctgatcgtg
     1441 gacaagccgc ccgggctggt ggtgcatccc agcgccggcc acgcttccgg cacgctggtc
     1501 aatgccctgc tgggacaggc tcgcgagcgt gggcagtcgc tgggatcggt ggcgggcgtc
     1561 ggacgcccgg gcatcgtcca ccggctggac aaggacacct ccgggctgct ggtcgtggca
     1621 cgcacggacg ccgcccaggc cagcctcatg cgccagttcg gtgaccgctc ggttggcaag
     1681 gagtacctgg ccctggtgcg gggagaggcc ccggcgcccc gcggccgggt cgaggcgccg
     1741 gtaggtcgcg acccgcgcga ccggcagcga atggccgtcg tggccggtgg ccgcgagtcg
     1801 gtgaccgagt acgaggtggt ggccaccggt ggcgggtact ccctcctcgc gctgcacccc
     1861 aggaccggcc gcacccacca gattcgggcg cacctcgcct acctgcgcct tccgatcgcc
     1921 ggcgacctgc gctacggcgg aggcatcggt cccggagggt tggagcgcca gttcctccac
     1981 gccgccgtcc tggagctgca tcggccgctt gacgggcggc ggctgcgtgc ctggagcggg
     2041 ctgccaccgg acctggccgc ctcgctggcg gcggctggcg tggacctgac gcgggccggg
     2101 agccggctgc cggtgggtag cggcgcggag gtggtgggct gaccgtggac gaggtgcagg
     2161 tcaagcggct ggccttcccc aacccgatgc tggtggtggt ctccggaccg cctggcgtgg
     2221 gcaagagcac catcgtcgcc gagcttgcgc gggggcaccc gcaggtcgtt cccatcgtga
     2281 cggtcaccac ccgccagcaa cggccggacg agaccgatgg cgtccactac cacttcgtct
     2341 cggatgccga tttccaggcc ctcatcgcgg ccgatggctt gctggagcat gccaacaacc
     2401 acggctactg gtacgggacg ccggtgaacc aggtgcgcgg catcctggcg gcgggtcgcg
     2461 atgcgatcct gaccatcagc cccgagggcg cccggaacgt gcgcgcccag gtcccgggcg
     2521 cgctcctcat cttcgtcatg ccaccgtccc tggatgacct ggacgagcgg atgcgtgccc
     2581 gcggctccga gacggttgag tccctggagc gccgccggcg ggatgccgaa cgctggatgg
     2641 ccgagaagga cgactacgac catgtcgtcg tcaacgagac ggggcacccg gagtgggcgg
     2701 ccgagcggat ctgggagatc atccaggccg aggcgcgacg ggagccgccg cgccaggtcc
     2761 ggatctgagg acggccagcc cgatgcccgc cgagccgggc cgcaacgtcc gggtggcggt
     2821 ggacgccatc gccgatcggc cggagcgcac cttcagctac ctgctgccgc ccgagctcga
     2881 cgctgccgaa cccgggtcgc tggtgctggt gccctacggc cgacgcctgg cgctcgggta
     2941 cctgatgccg ggcgaacccg aggcggtgga tggcgccgag ctgaaggcgg tggaggccgt
     3001 gctgtcggag ccgatgctca ccccggacct cctgcacctg gccgaggcga tcgccgagca
     3061 ctaccgggcg ccggtgggaa ccaccctggc cgccatgctg ccgcccggcc tggagtcccg
     3121 cctcgagcgg cgctgggagg tgctggaccc atctgccctg ccacctggcc tggcttctgg
     3181 agtggccagc gatgcagacg tcagccacgc ggccctgctg cgccacgcgc cgcgccgcgg
     3241 gcgcgaggca tgggtcgagc gcctgcgccg ttcgggagca atacgcgcgc gatggaccct
     3301 ccgccgcccg gacgtccagg cgcgcaccgt ccgcgtcctg cggccccttt ccggtggagt
     3361 tctgccggcg cgacgcgcgc ctctgcagcg cgcgatgctc gaagcgctgg gcggcgagga
     3421 acgcacgctc cccgagctgg ccgaggcgct gggcgtggat ctggcctcgc tgctcggtcc
     3481 tgcgcggcgg ctggcggcaa tcggtgctgc ggagctgagc gcccgggaag tggcccgcga
     3541 tccgctggcg catcgcgccc cggcgacatc ggtccgggag gggctggcgg ccgagcaggt
     3601 ggccgctctg caccagatcg agggactgcc gcccggcggg gagctgctgc tggagggagt
     3661 ggccgccgcc ggcaagacgg aggtctacct ggcggccgtg gccgccgttc taaaggcggg
     3721 cgagggtgcc gtgttgctcg tccccgagct gtcgatggtc ccgcagctgg cagaccgcgt
     3781 ggccggcgtg gtcggcgatc ggctggcggt gctgcattcc ggcctgtcgg cgggggagcg
     3841 gcacgacgag tggtggcgga tcctgcgcgg cgaggcgcgg gtggtagtcg gcacgcgcac
     3901 cgccgtcttc gcgccggtcc agcgcctggg cctgatcgtg gtggatgagg cgcacgacgg
     3961 cggctacaag tccgatcgca cgccgcgcta cgacgcgcgc tggtcggccg ctcgccgcgc
     4021 ggcccgcacc ggggcgcgac tggtgctggg gacggcgacc ccggacgtgg tcaccatcgc
     4081 tcgggtgcgg ggcgggatgg tggagcgggc gcggttgcga gagcgtcggg tgggccgcgc
     4141 cccggaggtc gaggtggtgg acctgcgggc cgagctggcc tccgggaatc gttccgtgtt
     4201 ctccgccgcg ctggcggccg ccctcggcga gctgcgggcg gggagcgagc aggccatcct
     4261 gctcatgaac cggcgcggtg cctccacctt catcctgtgc cgggactgcg gcgagagcct
     4321 gcgctgcccg gactgcgacc tgccattcgt cttccacgcg accgatgagc tcttgcgctg
     4381 ccaccactgc gggcgcagcg cccgcccgcc cgagcgctgc cctcgctgtg actcacgccg
     4441 catccgctac ttcggtgccg gcacgcagcg agtggaggcg gagctgcgga gccgctttcc
     4501 ggggcttcag gtggcccgcc tggactcaga cgcgctggcg gcacggcgcg gcttcgaggc
     4561 gatctacgac gacttccgcg acggccaggt ggacgtgctg gtcggcaccc agctggccgc
     4621 caaggggctg gacctgccgt cggttaccct tgccgccgtg atcgcggcgg acgtgacgtt
     4681 gaacctgccg gactacctgg cgccggagcg gaccttccag ctgctcgccc aggtcgccgg
     4741 tcgagccggg cgtggaccga tgccggggcg ggtcatcatc cagacctatg cgccggacca
     4801 ctacgcggtg aaggccgccg cccgcatgga cgtcgacggc ttcgccgatg aggagctcac
     4861 ccgacgccgg ttgctcggct acccgccggc atcggtgctc gcgcggctgt tgttggccga
     4921 tccggaccgg gagcgagccg aggcgcgggc cgaggcggct gcgtccgccg tccgcgttcc
     4981 gggtgtggag gtgcacgggc cactcgccgc ctacgtggcg cgcagcgctg gccgctggcg
     5041 gttccaggtg atcctgcgcg ccgagcgcgg cgctgaccgt cgcgccgccc tggaacgagt
     5101 gcccgccggc gtggggatcg acgtggatcc cgagtccctg ctgtgag
//
LOCUS       DATACA010000065         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2018017, whole genome shotgun sequence.
ACCESSION   DATACA010000065 DATACA010000000
VERSION     DATACA010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018017"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VHK28_01465"
     CDS             complement(<1..118)
                     /locus_tag="VHK28_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX2140928.1"
                     /translation="MSGHSKWSQIKRQKGANDAKRGALFTKLTREIITAARQG"
     gene            286..738
                     /locus_tag="VHK28_01470"
     CDS             286..738
                     /locus_tag="VHK28_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140929.1"
                     /translation="MEPLANPANAGVLRYLGQDGDPGELTVERPTPETDTWRLGAHPD
                     VIEWLWTHLNEGLEEDSRFLIVGGAALAHPRSGVILALALGTQYALRLSGNDFEAART
                     AGYQTAHEFRTVGRSLDLAATFGPGWVFGRHDPREKEWLGESWRAANL"
     gene            833..2905
                     /gene="fusA"
                     /locus_tag="VHK28_01475"
     CDS             833..2905
                     /gene="fusA"
                     /locus_tag="VHK28_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX2140930.1"
                     /translation="MKPTSPDRIRNVALISHGGAGKTSLAEAMLFDAGAISRMGSVDA
                     GTATLDWDPDEQKRQQTINLAIASLEQDDTRITIVDTPGYADYQADVVEALAAVDAAI
                     VVVDASAGVEVGTEEVWRLAEERKLPRFVFINKMDRENANYDAVLDQLKARFGPKIAP
                     VYLPIGSADSFRGYIDVIEEHANVYEGGTPKEVPIPDELQGAEHSRRDALVEAAAEAS
                     DELMMKYLEGEEITDAEIGAAMHKGTREGSVVPVFVGSALKNIGVRELIEMIVKHIPS
                     PAEVGTRTTPDGKEIKPDPAAPFVAQVFKTTADPFVGRLTYFRVVSGTLRSQGHVYNA
                     SRREEERIGNLLAMHGKDQSNLPQVGPGDIAAVAKLTSTQTGDTLVADRSQAVELPRP
                     VFPEPSLQVAIEPESKADLDKLGQALSRLQEEDPAVRVVREDATGETILTAMGDAHVD
                     VIVERLKRKFGASVKTSVPQVPYRESIRKPTKVDNKFKRQTGGHGQYGHVVIEFEPLE
                     ADRSFEFGDRIVGGVVPKQYIPAVEKGLREAMSEGVLAGFPVVGLRATLVDGSYHTVD
                     SSEMAFKIAASQALKRAFPEANPTLLEPVLEVEVVVPDEYMGDVMGQLTAKRGHVLGM
                     DSSNGTQRLRAQVPQAEMFQYATELRSITQGRGRFSWKLDHYAEVPHNLADKVIAERS
                     KVTAGEKH"
     gene            2929..>3158
                     /locus_tag="VHK28_01480"
     CDS             2929..>3158
                     /locus_tag="VHK28_01480"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HEX2140931.1"
                     /translation="MTPEERLERIGAWAAATPGVGAALVVGSRARTDVPADEHSDTDV
                     VLVCDDPDRWLHAIDWLAEFGRPLLSFVEETAV"
BASE COUNT          535 a         1085 c         1092 g          446 t
ORIGIN      
        1 caccctgtcg cgccgcggtg atgatctcgc gggtcagctt ggtgaacagc gccccgcgct
       61 tggcgtcgtt ggcgcccttc tgtcgtttga tctgggacca cttgctgtgg ccggacatcg
      121 gtcctccttc gtcatcccct ggatggcaag tctaccaagg aggtcagcca tccctgcccg
      181 gggccaaagg cgtttcgccc gattccacgt ttccgcggcc ccccacagga gcccgtgaca
      241 acttctggcc ggcccgtctg ccctccctgc ccctacgatg gaccgatgga gccgctcgcc
      301 aacccggcca acgcgggtgt gctgcgctac ctgggacagg acggcgaccc cggagagctg
      361 acggtggagc ggccgacgcc ggagacggac acctggcgac tgggcgcgca cccggacgtg
      421 atcgagtggc tgtggacgca tctgaacgaa ggcctggaag aggattcgcg cttcctgatt
      481 gtcggcggcg ccgcactcgc gcacccgcgc tccggcgtga tcctggcgct cgcgctcggg
      541 acgcagtacg cgctgaggct gtccggcaac gacttcgagg cggcacgaac ggccgggtac
      601 cagacggcgc acgagttccg gaccgtcggc cgcagcctcg acctggcagc gaccttcggc
      661 cccggatggg tcttcggccg acacgatccg cgggagaagg agtggcttgg tgagtcctgg
      721 cgcgcggcga acctataatc ggcccatcct cgagagctca atccccgccg ccggcggagg
      781 ctcggcggcg accgatgcac cgcgctgaac gaggcgcgca caaggagcac cgatgaagcc
      841 gacctccccc gaccgcatcc ggaacgtcgc cctcatctcc cacggcggcg ccggcaagac
      901 gagcctcgca gaggcgatgc tcttcgacgc cggcgcgatc agccgcatgg gcagcgtgga
      961 cgccggaaca gccaccctcg actgggaccc cgacgagcag aagcggcagc agaccatcaa
     1021 cctggccatc gccagcctcg agcaggacga cacgcggatc accatcgtcg acacccccgg
     1081 ttacgccgac taccaggccg atgtggtcga ggcgctggcc gccgtggacg cggccatcgt
     1141 ggtggtggat gccagcgccg gggtcgaggt cggcacggag gaggtgtggc gcctggcaga
     1201 ggagcgcaaa ctcccccgct tcgtgttcat caacaagatg gaccgcgaga acgccaacta
     1261 cgacgccgtg ctggatcagc tcaaggcgcg gttcggtccg aagatcgcgc cggtctacct
     1321 gcccatcggt tcggcggact ccttccgcgg ctacatcgac gtgatcgagg agcacgccaa
     1381 cgtctacgag ggtggtacgc cgaaggaggt ccccatcccg gacgagctgc agggcgcgga
     1441 gcattcgcgg cgcgacgcgc tcgtcgaggc ggctgccgag gccagcgacg agctgatgat
     1501 gaagtacctc gagggcgagg agattaccga cgccgagatc ggtgccgcaa tgcacaaggg
     1561 cacgcgcgag ggcagcgtgg tgccggtctt cgtggggagc gccctgaaga acatcggcgt
     1621 ccgcgagctg atcgagatga tcgtgaagca catcccctcc ccggccgagg tgggaacgcg
     1681 cacaacaccg gacggcaagg agatcaagcc cgatcccgct gccccgttcg tggcccaggt
     1741 cttcaagacc acggccgatc cgttcgtcgg ccgcctgacc tacttccggg tggtgtccgg
     1801 caccctgcgc tcgcaggggc acgtctacaa cgccagccgc cgcgaggagg agcgcattgg
     1861 caacctgctg gccatgcacg ggaaggacca gtcgaacctg ccacaggtgg ggcccggcga
     1921 catcgcggcc gtcgccaagc tgacctcgac ccagaccggg gacaccctcg tcgccgaccg
     1981 ctctcaggcc gtcgagctgc cacgccccgt cttcccggag ccgagcctgc aggtcgccat
     2041 cgagccggag agcaaggcgg acctggacaa gctgggccag gcgctcagcc gcctgcagga
     2101 ggaggatccg gccgtccgcg tcgtgcgcga ggacgccacc ggcgagacga tcctgaccgc
     2161 catgggcgat gcgcacgtgg acgtcatcgt ggagcggctg aagcgcaagt tcggtgcatc
     2221 ggtcaagaca tcggtcccgc aggtgcccta ccgcgagtcc atccgcaagc cgaccaaggt
     2281 cgacaacaag ttcaagcgcc agaccggcgg ccatgggcag tacgggcacg tggtgatcga
     2341 gttcgagccg ctggaggcgg accgtagctt cgagttcggc gaccggatcg tcggcggcgt
     2401 ggtgcccaag cagtacatcc cggccgtcga gaagggcctt cgcgaggcga tgtcggaggg
     2461 ggtcctggcc ggtttccccg tcgtcggcct gcgggccacc ctcgtggacg gcagctacca
     2521 caccgtggac agctcggaga tggccttcaa gatcgcggcc tcgcaggcgc tcaagcgcgc
     2581 cttcccggag gccaatccga ccctcctgga gccggtgctg gaggtcgagg tggtggtccc
     2641 cgacgagtac atgggcgacg tcatgggcca gctgaccgcc aagcgtggcc acgtgctggg
     2701 catggactcc tcgaacggga cgcagcgcct gcgcgcgcag gtgccccagg cggagatgtt
     2761 ccagtacgcc accgagctgc gctccatcac ccagggccgc gggcgcttct cctggaagct
     2821 ggaccactat gccgaggtgc ctcacaacct ggcggacaag gtgatcgccg agcgctcgaa
     2881 ggtgaccgcc ggcgagaagc actagaccga taccacccgg tcgcgccggt gacgcccgaa
     2941 gagcgcctgg agcgcattgg agcgtgggcg gccgcgacgc ccggcgtggg cgccgcgctg
     3001 gtggtcggct cgcgcgccag gaccgatgtg cctgcagacg agcactcgga caccgatgtc
     3061 gtcctggtct gcgacgaccc ggaccgatgg ctccatgcga ttgactggct cgccgagttc
     3121 ggccggccgc tgctgtcctt cgtggaggag accgcggt
//
LOCUS       DATACA010000066         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1536252, whole genome shotgun sequence.
ACCESSION   DATACA010000066 DATACA010000000
VERSION     DATACA010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536252"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VHK28_01485"
     CDS             <1..486
                     /locus_tag="VHK28_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX2140932.1"
                     /translation="LLFLTTGISADKWGNARRTAPSSGALYPIELYAVVHNVAGVDPG
                     VYHYALREHALEQVRAGDFRAQVVEQGISQEFLGECGVVLFLTQVLQRMRPKYQDRSY
                     RYGLLEAGHIGENAYLAATSAGLGACGVGAFMDDAINDMLGVDGTEEAVVYMLAIGHP
                     R"
     gene            483..1523
                     /locus_tag="VHK28_01490"
     CDS             483..1523
                     /locus_tag="VHK28_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX2140933.1"
                     /translation="MRTRAAALGAGLLPGLGLVLLVALPARVIGAVTPDVLSEITIAV
                     VVGVVTANLVALPDSFMAGIRFAVRVLLRVGIVFLGAALSFGAVVSTGLGALVVIVLS
                     MLLALGLTIVLGRLLGVPPRLALLVAVGTAICGNSAIVATAPVIEARDRDVSFAIGTI
                     TLFGLSAVLLYPAFGHLMGLSDEVFGTWAGVAVNDTSQVTAAGFAYSEAAGATATIVK
                     LTRNALMGPVILVIGAWYASWSRSGGSADRSGSGEGGRQRRARLSEMVPLFVIGFLAL
                     AVANSAGLIPPVAATAFEDLARFLILLALAGVGLSTNLREMRAVGLRPLYLGLGVAAV
                     LAVFSLALITAL"
     gene            complement(1538..1867)
                     /locus_tag="VHK28_01495"
     CDS             complement(1538..1867)
                     /locus_tag="VHK28_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140934.1"
                     /translation="MRALIVLPLILAACTAPGSREGGGEGGDTNGLTACESAMQLAAD
                     IDAAQEAHTDLDPAIQQCESIDEFEAAARRFPAALDGIPAREFLLNRCGAEPRVDLEL
                     VCAEVAQ"
     gene            complement(1876..2715)
                     /locus_tag="VHK28_01500"
     CDS             complement(1876..2715)
                     /locus_tag="VHK28_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX2140935.1"
                     /translation="MLVVVAIGVLLMGAINSLGPTDDDAALTRAAESATPQPHTPTSE
                     PEPTPEEQATSLPWASATPAPTAAPTVAPVLGATPIGPTQAATVERVIDGDTIEVRLG
                     GSLYTVRYIGIDTPETVHPSQPVEWMGPEASAVNTALVLGRNVVLEKDVSETDAFGRL
                     LRYVWIPQPDGLLLVNLKLVRRGFANASSYPPDVRYQDLLRTAEAEAREAGAGLWGEP
                     PPLTVAPPPAAGGCDPSYPDVCILAYPPDLDCGDVEFRRFAVVPPDPHGFDGDHDGIG
                     CES"
     gene            complement(2921..3274)
                     /locus_tag="VHK28_01505"
     CDS             complement(2921..3274)
                     /locus_tag="VHK28_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140936.1"
                     /translation="MNDGAGALNLLRLTVALFIVLSLILTFVAISVGVPYRVGGIGEI
                     ENVGADSFLRGTGLSAPIVFLIAFAVLLALTWLPTPWRAVPVFMIVVAAVVSSSPGRR
                     RFRWGAGPSPTTSVC"
     gene            3308..3778
                     /locus_tag="VHK28_01510"
     CDS             3308..3778
                     /locus_tag="VHK28_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140937.1"
                     /translation="MDGFLAFKYLHIVSMFFAVALAVSGDIVIRRVAATRDARAIRVV
                     TGRVRPLANLSTILFLAGVAFGIVAALTGQIDLLAPWLILAYVAFLTAMAIGITIADP
                     WVSRLEAASAGSPDGTPSESLQAVIDDPRARAGTWALMLLVATLVFLMVVKPFA"
BASE COUNT          627 a         1459 c         1516 g          701 t
ORIGIN      
        1 ctgctcttcc tgaccaccgg catcagtgcc gacaagtggg ggaacgcgcg acgcaccgcc
       61 ccgtcgtccg gcgcgctgta cccgatcgag ctgtatgccg tggttcacaa tgtcgccggc
      121 gtcgacccgg gcgtgtacca ctacgcgttg cgcgagcacg cgctggagca ggtgcgggcc
      181 ggcgacttcc gggcgcaggt ggtggagcag gggatctccc aggagttcct gggagagtgc
      241 ggcgtggtgc tgttcctgac ccaggtgctc cagcggatgc gcccgaaata ccaggaccga
      301 agctatcggt acgggctgct ggaggccgga cacatcggcg agaacgcgta cctggcggcg
      361 acctcggcgg ggcttggcgc ctgtggcgtg ggcgcgttca tggacgacgc gatcaacgac
      421 atgctcggcg tggacggcac cgaggaggcg gtcgtctaca tgctggccat cggccaccct
      481 cgttgaggac gcgcgctgcg gcgctcgggg ccggcctgct gccggggctc gggctggtgc
      541 tgctcgtggc gctgccggcg cgggtcatcg gtgcggtcac gccggacgtc ctgagcgaaa
      601 tcacgatcgc ggtggtcgtc ggcgtggtga ccgccaacct ggttgcgctg ccggacagct
      661 tcatggccgg tatccggttc gcggtgcgcg tcctcctgcg ggtggggatc gtgttcctgg
      721 gcgccgcgtt gagcttcggg gccgtggtca gcaccgggct gggcgcgctg gtcgtgatcg
      781 tcctgtccat gctgctggcg ctggggctga ccatcgtcct cgggcgcctg ctgggggtcc
      841 cgccgcgctt ggccctgctg gtggcggtgg gcacggccat ctgcggcaac tcggcgatcg
      901 tggccaccgc gccggtgatc gaggccaggg atcgggacgt gagcttcgcc atcggcacca
      961 ttaccctgtt cgggctgagc gccgtgctgc tctacccggc cttcgggcac ctgatggggc
     1021 tcagcgacga ggtcttcggc acgtgggccg gggtcgccgt caacgacacc agccaggtta
     1081 ccgcggccgg cttcgcctac tcggaggcgg ccggggcgac agccaccatc gtcaagctga
     1141 cgcgcaacgc gctgatgggg ccggtgatcc tggtcatcgg tgcctggtac gcgagctgga
     1201 gccgcagcgg tggctcggcc gaccgatcgg gctcgggtga gggcggtcgc cagcggcgag
     1261 cacggctgtc ggagatggtc ccgctcttcg tgatcgggtt cctggcactg gccgtggcca
     1321 actccgcggg tctgatcccg ccggttgccg cgacggcatt cgaggacctg gcgcgcttcc
     1381 tgatcctgct ggcgctggcc ggcgtggggc tgagcacgaa cctgcgcgag atgcgggcgg
     1441 tcgggctgcg gccgctctac ctgggactgg gcgtggccgc cgtcctggcc gtcttcagcc
     1501 tggcactgat cacggcgctg tagtcgtccg gcccgcccta ctgtgcaacc tcggcacaca
     1561 ccagctcgag gtcgacgcgc ggctcagcgc cgcaccgatt gagcaggaac tcacgcgccg
     1621 gaatcccatc gagagcggcg gggaaccttc gggcggcagc ctcgaattcg tcgatggatt
     1681 cgcactgctg gattgcagga tcgaggtccg tatgcgcctc ctgcgcggca tcgatgtccg
     1741 ccgccagctg cattgccgac tcgcaggcgg tcaggccatt ggtatcgcct ccctcaccac
     1801 cgccctcacg ggacccgggc gccgtgcacg cggcgaggat cagcgggagg acgatcagcg
     1861 cccgcatcgc cattcctagg actcgcagcc gatgccgtcg tgatcgccgt cgaacccgtg
     1921 cggatcgggc ggcaccaccg cgaacctgcg aaactcgacg tcaccgcagt ccaggtccgg
     1981 cgggtaagcc aggatgcaga catccgggta ggacgggtca cagcccccgg cagccggagg
     2041 cggggcgacg gtcagcggtg gcggctcacc ccacaggccg gccccggctt cgcgcgcctc
     2101 ggcctcggca gtgcgcagca ggtcctggta gcggacatcg ggcgggtagc tggaggcgtt
     2161 ggcgaagcca cgccgcacga gcttcaggtt gaccagcagc aggccatccg gctgggggat
     2221 ccacacgtag cgcagcaggc gcccgaaggc atcggtctcc gagacgtcct tctccaggac
     2281 cacgttgcgt ccaagcacaa gcgcggtgtt gaccgccgat gcctccggcc ccatccattc
     2341 caccggttgc gacgggtgga cggtctccgg cgtatcgatc ccgatgtacc ggacggtgta
     2401 caggctgccg ccaaggcgaa cctcgatggt gtcgccgtcg atcacccgct ccaccgttgc
     2461 cgcctgggtt ggaccgatgg gtgtggctcc gaggaccggc gcgacggtgg gtgccgccgt
     2521 tggcgctggc gtagcgctcg cccacggcag ggaggtggct tgctcttcgg gtgtcggctc
     2581 gggttccgac gtcggcgtgt gcggctgcgg cgtggcactc tcggccgcgc gggtcagggc
     2641 cgcgtcgtca tcggttgggc ccaggctgtt gatggctccc atgagcagca cgccgatcgc
     2701 gacgacgacc agcacacgct gggcgcgcga acgttgctgc caccagtggc tcggcacacg
     2761 cccctccctc cgacttcctg ggccaggccc gagggtagca ccgatgggcg ggactaaccc
     2821 cctccggcag tggcgcgacg gaccgactgg aagcccgcga tcagcccgac gacgatcatg
     2881 gccaccgccg aagccatgct cagggcccag atgatgaccg ctagcagacc gatgtcgtag
     2941 gcgaagggcc cgctccccaa cggaatctcc gccgtcccgg cgatgatgat acaacagcgg
     3001 cgaccacgat catgaagaca ggcaccgccc gccacggggt gggcagccag gtgagggcca
     3061 gcaggaccgc gaaggcgatc aggaacacga ttggcgccga gaggccggtg ccccgcagga
     3121 acgagtccgc ccccacgttc tcgatctcgc cgatgcctcc gaccctgtac ggcacgccga
     3181 ccgaaatcgc cacgaatgtg aggatcagcg agagcacaat gaacagagcg accgtgagcc
     3241 gcagcaggtt gagggcgccg gctccgtcgt tcatcggtca agcctagcaa ggagagcgca
     3301 ttcgatcatg gacggcttcc tggcgttcaa gtacctccac atcgtctcga tgttcttcgc
     3361 cgtcgcgctg gcggtcagcg gggacatcgt cattcgccgc gtggcggcca cacgcgacgc
     3421 cagggccatc cgtgttgtaa cgggaagggt gcgacccctc gccaacctgt cgaccatcct
     3481 cttcctggcc ggcgtggcct tcggcatcgt ggcggcgctg accggccaga tcgacctcct
     3541 ggcaccatgg ctgatcctgg cgtacgtcgc gttcctcacg gcgatggcga tcggcatcac
     3601 gatcgctgac ccatgggtga gccgcctgga ggcggcgtcc gctggcagcc cggacggcac
     3661 gccatccgag tcgctgcagg cggtcattga cgaccctcgt gcccgggccg gcacgtgggc
     3721 cctgatgctg ctggttgcca cgctcgtctt cctcatggtg gtcaagccct tcgcctgagc
     3781 tgtggcgtcc gccggcttct ttgccgaatc cacgcccgcg gcgcccttaa gtcggaagtt
     3841 gcccggggtt ctttgcctta cgcggccgca ggcggccctt agccagttgg ccacgcttac
     3901 tgcgcatggc tccacgcacg cggtatttgg ctaataccgc cgccggcggg cctatggcca
     3961 agaagtggcc ctggatggac cgataagccc ggccggcgac ggtatctggc agggttggcc
     4021 gcgggccgca gcggtgacgg agccacgacc cgccggccag ctacgatgtc tgtgccactc
     4081 cgaccgcacg atctcacgga tcggctcacc gcggcgttgg aatgggcatc gggcggtggc
     4141 cgccgagggg ccgccgtcac gcacactcac agacaggtga aactcgaatg actcgccgtc
     4201 cctcgccagt cgaatggctc gtgttcggag ccctgggctt cgcatggggc agcagctacc
     4261 tcttcatcaa gatcggcgtc gagacgctga cgccgctgac cct
//
LOCUS       DATACA010000067         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_572569, whole genome shotgun sequence.
ACCESSION   DATACA010000067 DATACA010000000
VERSION     DATACA010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572569"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VHK28_01515"
     CDS             <1..392
                     /locus_tag="VHK28_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140938.1"
                     /translation="TVNREVFEEVGEIGLAPEVHERIVSWRARFGLAIDNALNFLNNP
                     DGIQGHNFGTQVSHTDGGTALQGRLTLVRYNNEPQFFIADADILLSIAADVMDRLGEH
                     LDAEMVDPELVQRLREGVERIERRAKA"
     gene            389..1495
                     /locus_tag="VHK28_01520"
     CDS             389..1495
                     /locus_tag="VHK28_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2140939.1"
                     /translation="MSGARRIFLAATGQNRGKTTVSLGLAAGIAQRGHRLGFLKPVGQ
                     RYIMVDDVPADEDAVLMREVFGLTDALALMSPVTLPRGFTTDFVMGRIAADLGASVAA
                     AERTLAEDKDVVLIEGTGHAGVGAVVGLSNAQVAAMLEAPVIIVSEGGVGRPIDEIVL
                     NQALFQRHGVRILGAVVNKVNVAANPRLPEVLQRGLAQHGVELLGCLPYSELLANPSL
                     ELVATHLAGELLSGAATPGQTIGQVAIGAMQADHAVPFLRNRTLLITPGDRDDMLEVT
                     LEANRGAVAADRPRRVAGVVLTGGFRPSPSRLAQLRDAGLFVLLVEGDTYATAQSVHD
                     ILVKTHAGDTEKIETIIELVAGALDVDGLLERVG"
     gene            1527..2120
                     /locus_tag="VHK28_01525"
     CDS             1527..2120
                     /locus_tag="VHK28_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEX2140940.1"
                     /translation="MTAILNRQPDRLHYTGDDDADRLLASDPFALLVGFALDQQVTVQ
                     KAFSGPTEITRRLGTLDPARVAAMDPAELSATFRQRPAIHRFPDAMARRVHALARFIV
                     DRYGGDASRIWSEATDGADLRRRLAELPSFGDMKVRSMLAVLAKRFGLKLPGLDEVVP
                     QHPTLGDVDSAEALDAYQAAKRAHKAELREAGKRASG"
     gene            complement(2239..>2384)
                     /locus_tag="VHK28_01530"
     CDS             complement(2239..>2384)
                     /locus_tag="VHK28_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2140941.1"
                     /translation="RTDYVSVADANTLAELQRVSGPALAMVAAWIGRTRLVDNTPLAW
                     DVA"
BASE COUNT          368 a          817 c          855 g          344 t
ORIGIN      
        1 tgaccgtcaa ccgcgaggtg ttcgaggagg tgggggagat cgggttggcg ccggaggtgc
       61 acgagcggat cgtctcctgg cgggcgcggt tcgggctggc catcgacaac gccctgaact
      121 tcctgaacaa cccggacggc atccagggcc acaacttcgg cacccaggtc tcgcacacgg
      181 acggcggaac ggcgctccag gggcgcctga cgctggtgcg ctacaacaac gagccccagt
      241 tcttcatcgc cgatgccgac atcctgctat ccatcgccgc cgacgtgatg gaccgcctgg
      301 gcgagcacct ggacgccgag atggtcgacc ccgagctggt gcagcgcctg cgcgagggcg
      361 tggagcggat cgagcgccgc gccaaggcgt gagcggcgcg cggcgcatct tcctggccgc
      421 gaccgggcag aaccgcggca agacgaccgt gtcgcttggg ctggccgccg gcatcgccca
      481 gcgcggtcac cggctcggct tcctcaagcc ggtcggccag cgctacatca tggtcgacga
      541 cgtgccggca gatgaagacg cggtgctgat gcgcgaggtc ttcggattga ccgatgcgct
      601 ggccctgatg agcccggtca ccctgccgcg cggcttcacc accgacttcg tcatggggcg
      661 cattgccgcc gatctcgggg cgtcggtggc ggctgcggaa cggaccttgg ccgaggacaa
      721 ggacgtggtc ctgatcgagg gcaccggcca tgccggggtg ggcgccgtgg tcggcctgtc
      781 caacgcccag gtggccgcca tgctcgaggc gccggtgatc atcgtcagcg agggcggtgt
      841 cggtcgcccg atcgacgaaa tcgtgctgaa ccaggccctc ttccagcgtc acggggtgcg
      901 gatcctgggc gcggttgtca acaaggtgaa cgtggccgcc aacccgcgac tgccggaggt
      961 gctgcagcgc ggcctggccc agcacggcgt ggagttgctg ggctgcctgc cctactcgga
     1021 gctgctggcc aacccgtcgc tggagctggt ggccacccac ctggcggggg agctgctgtc
     1081 gggagcggcg accccgggcc agaccatcgg ccaggtcgcc atcggtgcaa tgcaggcgga
     1141 ccacgcggtg ccattcctgc gcaaccggac cctgctgatc acccccggcg accgcgacga
     1201 catgctggag gtcaccctgg aagccaaccg gggagcggtc gcggccgacc gtccgcgtcg
     1261 ggtggccggt gtcgtgctca ccggcggctt ccgtccgtcg ccatcgcgcc tggcccagct
     1321 gcgagatgcc ggcctcttcg tcctgctggt cgagggtgac acctacgcca ccgcgcagtc
     1381 ggtccacgac atcctggtca agacgcacgc cggcgacacg gagaagatcg agacgatcat
     1441 cgagctggtg gccggtgcgc tcgacgtgga tgggctgctg gagcgggtgg gatgacctgc
     1501 tggcgcggcc gggtaccatc actcatatga ccgccatcct gaaccggcag ccggaccggc
     1561 tgcactacac cggtgacgac gacgccgacc ggctgctggc ctccgacccc ttcgcgctgc
     1621 tggtcgggtt cgccctggac cagcaggtca ccgtccagaa ggccttctcc gggcccaccg
     1681 agatcacccg acgcctcggc acgctggacc cggcacgtgt ggcggccatg gatcctgccg
     1741 agctctcggc cacctttcgc cagcgacccg ccatccaccg cttcccggac gccatggccc
     1801 ggcgcgtcca cgcgctggcg cgcttcatcg tggaccgata cggtggcgac gcctcgcgga
     1861 tctggtcgga ggcgaccgat ggagcggacc tgcgacgcag gctggcggag ctgccatcgt
     1921 tcggcgacat gaaggtgcgc tccatgctgg ccgtgctggc caagcgattc gggctgaagc
     1981 tgcccggcct ggacgaggtg gtgccacagc accccaccct gggggacgtg gactccgccg
     2041 aggcgctcga cgcgtaccag gccgccaagc gggctcacaa ggccgagctg agggaggccg
     2101 gcaagcgggc ctcgggctga gggcacgagg gcagcggcca acaaatagaa gaggaggccg
     2161 agcgtgggca ctcgccggcc tcccaagcgc gaatcatacg tatgcggggc gggacgtcac
     2221 tagtactaac ggagaatttc aggcgacgtc ccaggccagc ggcgtgttgt ccaccaggcg
     2281 cgtgcggccg atccaggccg cgaccatggc cagcgccggg ccggagacgc gctgcagctc
     2341 ggccagggtg ttcgcatcgg ccacgctgac gtagtcggtt cggg
//
LOCUS       DATACA010000068         8898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2018758, whole genome shotgun sequence.
ACCESSION   DATACA010000068 DATACA010000000
VERSION     DATACA010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018758"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VHK28_01535"
     CDS             <1..285
                     /locus_tag="VHK28_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX2140942.1"
                     /translation="LAAAHAACFCMALSAGLARAGTPPEHLHVEAEVTFDKVGDAWTV
                     TASKLILLGRVPGISEADFNAAAEEAKDGCPISRALKGNVQLSVEPTLES"
     gene            complement(441..710)
                     /locus_tag="VHK28_01540"
     CDS             complement(441..710)
                     /locus_tag="VHK28_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140943.1"
                     /translation="MIVKPSIRTGFELESVLSAAEAVARRVALKQRDARLEAMVLVLA
                     DTRANRAAVAAARATLQGAFPTNARAVLAELRAGRSPQGNGILLV"
     gene            771..1145
                     /locus_tag="VHK28_01545"
     CDS             771..1145
                     /locus_tag="VHK28_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140944.1"
                     /translation="MSRQFDRLKLLLPGHFVWRSEVPLAIPGDQRAIDAMIVKPVIRT
                     GFELESLLSAAEAVARRVALKQRDAGLEAMVLVLADTRANRAAVTAARATLQGAFPTS
                     ARAVLAELRAGHSPQGNGILLV"
     gene            complement(1304..1747)
                     /locus_tag="VHK28_01550"
     CDS             complement(1304..1747)
                     /locus_tag="VHK28_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX2140945.1"
                     /translation="MAAVRTATASWSGDLATGDGTVTAGTTGLFTDLPVSWGSRTEAP
                     AGRTSPEELLAAAHAACFCMALSAGLARAGTPPEHLHVEAEVTFDKVGDAWTVTSSKL
                     ILLGRVPGISEADFNAAAEEAKDGCPISRALKGNVQLSVEPTLES"
     gene            1806..2417
                     /locus_tag="VHK28_01555"
     CDS             1806..2417
                     /locus_tag="VHK28_01555"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2140946.1"
                     /translation="MSDGQWPPVHPVPRITAERVYLRPAERDDLPRFVRWFADAEVAR
                     HLAVRAPFSLGMEEKWFDGMVERQGKTDYHFVVCLLADGRPIGTAGLHGLDLENGNAE
                     FGIALGEKDEWDKGYGTEALQAICDFGFGALRLERIDLHVYEGNARGRRSYEKAGFRM
                     EGRLRHAHFAEGRHEDVVVMALLREEWEALPRRKGWELGPPTR"
     gene            2414..3517
                     /locus_tag="VHK28_01560"
     CDS             2414..3517
                     /locus_tag="VHK28_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140947.1"
                     /translation="MSRISELPELIAGLPAASRDRAERMFRAEVVVGRTDPPPELHPW
                     LERQFGSVEAVLEQLITRVTNRWTQDGALFSPLRSRRPKDDNEESDGGSAATDGYRDG
                     EGSDPFCHPLTATPADTFGRIESAHAITGANAAKYDGLHGVVVFRRHHPLDFDAGTVV
                     DLLDIGRRWAEAAHDTDPEANAYLLIWNAGPRAGGSIVHGHAQVMLGRPPHFPQVERL
                     RRDAARYHDETGHAYLPDLVAVHRDLGLVVAETGGVATLASLTPVKERELLVIGPPGM
                     DERDPAFASVVARTVVAYRDRLGVRAFNLALHRPPISGAEGWEEIGPVVHLVDRGDPE
                     SRSSDIGAMELYAASVVGGDPFELADRLRSVLA"
     gene            3745..4731
                     /locus_tag="VHK28_01565"
     CDS             3745..4731
                     /locus_tag="VHK28_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140948.1"
                     /translation="MFELRRDPVTGWWSAVVTDRSFEHASFAVQAAPVEGTHCRHCDD
                     DPTSEADAREDTTVRRVTLRADAFHRIDSSKETAAQLSLSEVESSTGSWEILVAPRGH
                     HERLADASPRLALNFMRSVRDAMRQLATTAKPPDHPLSIQVVQSYGRQAGSRSQHLNV
                     ELYAIPQVPHWLAEEIGGGARHQIRTRTCVWCGLARAEEESGERLILAEERFVVFAPA
                     ASRSAFELMIVPRAHAADFTQLDDDDLAASAATIQRSLRALDVLGDPPYNLFLHTAPA
                     GERLDQTFHWHWELHPRLRVIAGLERATALAVNPAAPEYAADILRRHLAERT"
     gene            4835..5404
                     /locus_tag="VHK28_01570"
     CDS             4835..5404
                     /locus_tag="VHK28_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2140949.1"
                     /translation="MLSIGLEQGADAWTSPVSATPDRAAFDRLFEEFSAPIYNYVLRM
                     VGDADRAADITQDTFIKAYRKLDSLTDESSTRSWLYRIATNTAIDEMRRRRWIAPRGD
                     DEPPDRADERPGPEAQVMAGTLDDRIQRALMRLRPNHRQCLVLSDVEDMSAQQIGEVM
                     GLSYGAVRTLLCRARGEMRRLLAKEGLAR"
     gene            5401..7002
                     /locus_tag="VHK28_01575"
     CDS             5401..7002
                     /locus_tag="VHK28_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX2140950.1"
                     /translation="MTQPGNRPGQHPDELLSASLSGDLTGAERTALDAHLAGCQECTR
                     TLADFRRGRELLGAMRHVEPPRDMPARVYAAVDAPRAPWWRRPSTMVAALATGATMVA
                     ALLAVLTFNDLRQPEVGNVTPSPTAVVSMTPVPPAETPSAVVSPSEAPLPTPLEVAAH
                     FRSSLEQQDASLEIVTPRGTKDLLDTAALPVSSAVSPDGTWIAFRVEGESSGIAQTYV
                     TGDSGNEIHDLGRSVAPSSGVGGELAWSPDGRYLAYTLTSMQDGGSDAWLFDTTSSNV
                     QQVTGTGDSFAASWVPSTDGPMQLWISRASAQPVSFLVEIPVDGPPAQPVDPAQVAVQ
                     TVEGVFGPLVAPDGQKVIFWTGSMANLDGRWVFQPGTPMLGEADEAGALDFSGRPLFS
                     SLTVVGGEAFAAAQIAWGPDSDAFAVWNAEWTGIQQPDGFPARDRVYFGHVSVPELIT
                     PRQALDAADLEGVQQVIDVVIAPDGMKLAVTVQTASGSEGGEFGAEAEIRVIDRGLGE
                     VPDQVTVLKDAGSWNGPAVFDEWDG"
     gene            7034..7588
                     /gene="pyrR"
                     /locus_tag="VHK28_01580"
     CDS             7034..7588
                     /gene="pyrR"
                     /locus_tag="VHK28_01580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX2140951.1"
                     /translation="MSERPVLDADDLRRALTRIAHEIVERNGGTDGLVLVGVRSRGVP
                     LARRLAALIEQHEGVSIPVAALDITFYRDDLTRIAHAPIVKRTDDLPEIDDRTVVLVD
                     DVLYTGRTVRAALDALTDHGRPRAVRLAVIVDRGHRELPIRADHVGKNLPTAREELVH
                     VRLTETDDAEEVVLERAYTPPRNL"
     gene            7619..8575
                     /locus_tag="VHK28_01585"
     CDS             7619..8575
                     /locus_tag="VHK28_01585"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX2140952.1"
                     /translation="MIETEPALARRHRPADAWPHRHLLDVDALSRAEVERCLDLGREM
                     RQLHERGDRLDALAGHVVGLAFAEASTRTRVSFEIAAGRLGAQVVELPVEASSVTKGE
                     SLTDTLRTLERTGVGTVVLRHAASGAPYLAARATGLRVVNAGDGAHAHPTQALLDALT
                     LVEALDGLDGRTVTIVGDLAHSRVARSNLHLLQLLGARVRLAGPPAWVGAFRSWDGVD
                     VTTNLEEALDGTNAVMALRVQLERDAGGGVPSLREYARRWGLDESRMRRAAPGAPLLH
                     PGPTNEGVEVSADLAAGPRSLIGRQVANGVAVRMAVLALLAQ"
     gene            8572..>8898
                     /locus_tag="VHK28_01590"
     CDS             8572..>8898
                     /locus_tag="VHK28_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2140953.1"
                     /translation="MTRFGSISDPPTALLITGARVLDPAAESDAVRDLAIAGGRLVEP
                     ADLPREAARVDGSGLLAIPALCDLHSHLRDPGGEAAETLESGSQAAAHGGYASVCAMP
                     NTEPALD"
BASE COUNT         1299 a         3140 c         3170 g         1289 t
ORIGIN      
        1 ctggccgcgg ctcacgctgc ctgcttctgc atggcgctgt ccgccggcct ggctcgcgcc
       61 ggaacgccgc cggagcatct tcacgtcgag gcggaggtca ccttcgacaa ggtgggggac
      121 gcctggacgg tgaccgcgtc gaagctgatc ctgctgggcc gcgtgcccgg catctcggag
      181 gccgacttca acgcggcggc ggaggaggcc aaggacggct gccccatctc gcgcgcgctg
      241 aagggcaacg tccagctctc cgtggagccc acgctcgagt cctgaccgcc gcggcggcgc
      301 gacacgcggt caggatgtga ggcggccgat gacactctcg cctcggtcgt ccgtcggtcg
      361 aggcacgcgc agtgtgcgtg ggccgtcgga tcatggtggc tccgccggtc ctgtcacccg
      421 gtccgcgggt acgcgtgggc ctagaccagc aggatgccgt tgccctgcgg cgaacggccc
      481 gcacgaagct ccgccaggac ggcgcgtgcg ttcgtgggga aggcgccttg aagggttgcg
      541 cgggccgcgg ccacggccgc tctgttcgcc cgcgtgtcgg ccagaacgag gaccatcgcc
      601 tcgagccgcg cgtcgcgttg cttgagggcc acgcggcgtg ccacggcttc cgccgccgag
      661 agcacggatt ccagctcgaa gccggtccgg atcgacggtt tcacgatcat ggcatcgatc
      721 gcccgctgat ccccctggat cgccaacgga acctccgatc gccagacgaa atgtcccggc
      781 agttcgacag gctgaagctg ctattgccgg ggcatttcgt ctggcgatcg gaggttccgc
      841 tggcgatccc gggggatcag cgggcgatcg acgccatgat cgtgaagccg gtgatccgga
      901 ccggcttcga gctggaatcc ctgctctcgg cagcggaagc cgttgcacgc cgtgtggccc
      961 tcaagcaacg cgacgcgggg ctcgaggcga tggtcctcgt tctggccgac acgcgggcga
     1021 acagagccgc cgtgaccgcg gcccgcgcaa cacttcaagg cgccttcccc acgagcgcac
     1081 gcgccgtcct ggcggagctt cgtgcgggcc attcgccgca gggcaacggc atcctgctgg
     1141 tctaggcaca cgcgtactcg ccgagcgggt gacaggaccg gcggagccac gatgatccga
     1201 cgccccacgc accctgcgcg tgcctaaacc gagggacgac ccaggcgaga ccgtcgtcgg
     1261 ccgcctcaca tcctgaccga gtaaatgggt gtcgccgagg cgctcaggac tcgagggtgg
     1321 gctccacgga gagctggacg ttgcccttca gggcgcgcga gatggggcag ccgtccttgg
     1381 cctcctccgc cgccgcgttg aagtcggcct ccgagatgcc cggcacccgg cccagcagga
     1441 tcagcttcga cgaggtcacc gtccaggcgt cccccacctt gtcgaaggtg acctccgcct
     1501 cgacgtggag gtgctccggc ggcgttccgg cgcgagccag gccggcggac agcgccatgc
     1561 agaagcaggc agcgtgagcc gcggccagca gctcctccgg cgatgttcgt ccggccggtg
     1621 cctcggtgcg cgagccccag ctgacgggaa ggtcggtgaa caggccggtg gtgccggcgg
     1681 ttacggtgcc gtcgccggtg gcgaggtcgc cggaccagct ggcggtggcg gttcggactg
     1741 cggccatggt gggtatcggt cctcctacaa tcctcgggcg cgttgcgtct agcctagagg
     1801 gaccgatgtc agacggccaa tggccccccg tccaccccgt gccccgcatc accgccgagc
     1861 gggtgtacct gcgtcccgcg gagcgcgacg acctgccccg cttcgttcgc tggttcgccg
     1921 atgccgaggt ggcccgccac ctcgcggtcc gtgcgccatt cagtttgggc atggaggaga
     1981 agtggttcga cgggatggtc gagcgccagg gcaagacgga ctaccacttc gtggtgtgcc
     2041 tgctggccga tggccggccg atcggaacgg ccggcctcca cggactggac ctggagaacg
     2101 ggaacgcgga gttcggcatc gccctgggcg agaaggacga gtgggacaag ggctacggga
     2161 ccgaggctct gcaggccatc tgcgatttcg gattcggcgc gctgcgcctg gaacggatcg
     2221 acctgcacgt gtacgagggc aacgcgcgtg ggcggcgctc gtacgagaag gccggcttcc
     2281 gaatggaggg ccgcctgcgt cacgcgcact tcgcggaggg gcggcacgag gacgtggtgg
     2341 tcatggccct gctgcgagag gaatgggaag cgctgccgcg ccggaagggc tgggagctgg
     2401 gaccgccgac ccggtgagcc gcatatccga gctgccggag ctgatcgccg gcctgccggc
     2461 cgcatcccgc gaccgggcgg agcgcatgtt ccgcgccgag gtggtggtgg gccgcaccga
     2521 tcccccgccc gagctgcacc cctggctgga gcggcagttc ggatcggtcg aggcggtgct
     2581 cgagcaactg atcacccgcg ttaccaacag atggacgcag gacggcgcgc tcttctcgcc
     2641 gctccgctcg cgccggccga aggacgacaa cgaggaatcg gacggcggat cggccgccac
     2701 cgacgggtac cgggacggcg aaggcagtga tccattctgc catcccctga ccgcgactcc
     2761 ggcggacacg ttcggtcgca tcgagagcgc gcacgccatc accggggcca acgccgccaa
     2821 gtacgacggc ctccatggcg tggtggtctt tcggcggcat cacccgctcg acttcgatgc
     2881 cggcacggtc gtggacctgc tcgacatcgg gcgccgatgg gcggaggcgg cgcacgatac
     2941 cgacccagag gcaaacgcgt acctgctgat ctggaatgcg ggacctcgtg ccggtggatc
     3001 gatcgtgcac ggccacgcac aggtcatgct tggtcgcccg ccgcacttcc cccaggtgga
     3061 gcgactgcgt cgcgatgcgg cccgctacca cgacgagaca ggtcacgcct acctgcccga
     3121 cctggtggcc gtgcatcgcg acctgggact ggtggtggcc gagaccggtg gcgtcgccac
     3181 gctggcctcg ctgacgccgg tcaaggagcg tgagctgctg gtgatcgggc cgcccggcat
     3241 ggatgagcga gatcccgcat tcgcctccgt cgttgcgcga acggtggtgg cctaccggga
     3301 ccggctcggg gtccgggcct tcaacctggc gctgcatcgg ccaccaatct ccggggcgga
     3361 aggctgggag gagatcggtc cggtcgtcca cctcgtggac cgcggggatc cggagagccg
     3421 cagctcggac atcggtgcca tggagctgta tgccgcatcg gtggtcggcg gcgacccgtt
     3481 cgagctggcg gaccgcctgc gatcggtcct cgcctgaggg cccgcccaca cggcgggcag
     3541 gagttcacgg gaggccgccg cagcgtgctg tcctgccggt acagcaggca gcgttgagcg
     3601 tgagcggcga agttcttgtc cgttcgtgcg tggccctgcc cgacacatcg gcgatctgac
     3661 acatgggctg gcattcctgt gaaggagtgc tcgaaatatc gggcaccgaa ggccacatga
     3721 ccgccatcaa gtacgatgcg cgccgtgttc gagctgcgcc gcgaccccgt caccggctgg
     3781 tggtcggccg tggtcaccga tcgcagcttc gagcatgcct ccttcgcggt gcaggccgcc
     3841 ccggtggagg gcacgcactg ccgccactgc gacgacgacc cgacgtccga ggccgatgcc
     3901 cgcgaggaca ccaccgttcg acgggtgacg ctgcgggccg atgccttcca ccgcatcgac
     3961 tcctccaagg agacggctgc ccagctgtcg ctgtccgagg tggagtcatc gactggatcg
     4021 tgggagatcc tggtcgcgcc gcgaggccat cacgagcggc tggctgacgc ctcgccccgg
     4081 ctggcactca acttcatgcg ttcggtgcgc gatgccatgc ggcagctggc gacgaccgcc
     4141 aagccgccgg accatccact atccatccag gtggtgcaga gctacggacg ccaggccggc
     4201 tcgcgcagcc agcacctgaa cgtcgagctg tacgccatcc cgcaggtgcc ccactggctg
     4261 gccgaggaga tcggcggcgg ggcgcgccac cagatccgga cgcgcacctg cgtgtggtgc
     4321 ggcctggcac gtgccgagga ggagtcgggc gagcgcctga tcctcgccga ggaacggttc
     4381 gtggtattcg ccccggcggc cagccgcagc gccttcgagc tcatgatcgt gccccgcgcc
     4441 cacgcggccg acttcacgca gctcgacgac gacgacctcg ccgccagcgc cgccaccatc
     4501 cagcgctccc tccgggcact ggatgtcctg ggcgaccctc cgtacaacct cttcctgcat
     4561 actgcgcccg ccggcgagcg cctggaccag acgttccact ggcactggga gctgcacccg
     4621 cggctgcggg tcatcgccgg gctcgagcgg gcgaccgcgc tcgccgtcaa cccggccgcg
     4681 ccggagtacg cggccgacat cctgcgccgg cacctcgccg agcgtaccta ggaccagcca
     4741 caccacacca gccatagcac ggggtcgcgt aacaggggtg gagagcgatg cgtcacgagg
     4801 tacggacggg ggacgtctcg aagcggagga ccgtgtgctg tcgatcggcc tggagcaggg
     4861 ggccgatgcc tggacgtcgc ccgtgagcgc gacgccggat cgtgccgcgt tcgaccgcct
     4921 cttcgaggag ttctccgcac ccatctacaa ctacgtcctg cgcatggtgg gcgacgctga
     4981 ccgagccgcc gacatcacgc aggacacctt catcaaggcc taccgcaagc tcgactccct
     5041 gaccgatgaa tcctcgaccc gctcctggct ctaccggatc gccaccaaca ccgccatcga
     5101 cgagatgcgg cggcggcgct ggatcgcacc ccggggtgac gacgagccgc ccgaccgcgc
     5161 cgacgagcgc cccgggccgg aggcgcaggt gatggccgga acccttgacg atcgcatcca
     5221 gcgggcgttg atgcgcctgc gccccaacca tcggcagtgc ctggtcctgt ccgacgtcga
     5281 ggacatgagc gcccagcaga tcggcgaggt gatggggctc tcgtacggcg cggtgcgcac
     5341 cctgctgtgc cgtgctcgcg gcgagatgcg ccgactgctg gccaaggagg gactggcccg
     5401 atgacccagc caggcaaccg ccccggccag caccccgacg agctgctcag cgcgtcgctg
     5461 agcggtgacc tgaccggtgc cgaacgcacg gcgctggatg cgcacctggc cggctgccag
     5521 gagtgcaccc gaaccctggc cgacttccgc cgcggacggg agctgctggg cgccatgcgc
     5581 cacgtcgagc cgccgcgtga catgccggcg cgcgtgtacg ccgccgtgga tgcgcctcgc
     5641 gccccgtggt ggcgccgccc gtccacgatg gtcgctgcgc tggcgaccgg cgcaaccatg
     5701 gtggctgcgc tgctcgccgt gctgaccttc aacgacctgc gtcagccgga ggtcggaaat
     5761 gtcaccccca gcccgaccgc ggtggtgtcc atgacgcccg ttccacccgc tgagaccccc
     5821 agcgcggtcg tttccccgag cgaggcgccg ctgccgacac cgctcgaggt ggcggcccac
     5881 ttccgctcca gcctcgagca gcaggacgcg tccctcgaga tcgtgacgcc gcgcggcacc
     5941 aaggacctgc tcgacaccgc ggctttgccg gtgagcagcg ccgtctcacc ggatggcacc
     6001 tggatcgcct tccgcgtgga gggtgaatct tcggggatcg cccagaccta cgtgaccggc
     6061 gactccggca acgagatcca tgacctcggc cggtcggtgg cgccatcctc gggcgtgggc
     6121 ggcgagctgg cctggtcgcc ggatgggcgc tacctggcct acacgctgac atcgatgcag
     6181 gacggcggca gcgacgcctg gctcttcgac accacctcca gcaacgtcca gcaggtgacc
     6241 ggcactggtg actcgttcgc cgccagctgg gtcccatcca cggacggacc gatgcagctg
     6301 tggatcagcc gcgcctccgc ccagcccgtc tctttcctgg tcgagatccc cgttgatggc
     6361 ccgccggcac agccggtcga cccggcgcag gtggcggtgc agacggtgga gggcgtgttc
     6421 gggccactgg tcgcgccgga cggccagaag gtcatcttct ggacgggcag catggcgaac
     6481 ctggatggca ggtgggtctt ccagccgggt acgccgatgc tgggcgaggc cgacgaggcc
     6541 ggcgccctcg acttcagcgg gcggccgctc ttctcctcgc tgaccgtggt gggtggcgag
     6601 gccttcgccg cggcgcagat cgcctggggg ccggacagcg acgcgttcgc ggtgtggaac
     6661 gccgagtgga ccggcattca gcagccggac ggcttccccg cccgcgaccg cgtctacttc
     6721 ggccacgtga gcgtgcccga gctcatcacg ccccgccagg cgctggacgc cgccgacctg
     6781 gagggcgtgc agcaggtgat cgacgtggtg atcgcccccg acgggatgaa gcttgcggtg
     6841 acggtccaga ccgcgtcggg ctcggagggc ggcgagttcg gggcggaggc ggagatcagg
     6901 gtcatcgacc gcgggctggg cgaggtcccg gaccaggtca ccgtcctcaa ggacgccggt
     6961 tcctggaacg ggcctgccgt cttcgacgag tgggacggct gaccggccgc tccatcggtc
     7021 tacactccgg agcgtgagcg agcgccccgt cctggatgcg gatgacctgc gccgcgcgct
     7081 gacccgcatc gcacacgaga tcgtcgagcg caacggaggg accgatgggc tggtcctggt
     7141 gggagtgcgc agccgaggcg tgccgctggc tcgccgcctg gcggcgctga tcgagcagca
     7201 cgagggggtc agcatcccgg tcgctgcgct ggacatcacc ttctaccgcg acgacctgac
     7261 ccggatcgcg catgcaccga tcgtaaaacg caccgacgac ctgccggaga tcgacgaccg
     7321 aacggtggtc ctggtggacg acgtgctgta caccgggcgc accgtgcgcg ccgctctgga
     7381 tgcgctgacc gaccatggac gcccacgcgc cgtacggctg gccgtgatcg tggaccgcgg
     7441 gcaccgggag ctgccgatcc gcgccgacca cgtcggcaag aaccttccga ctgcccgcga
     7501 ggagctggtg catgtccggc tcaccgagac cgatgacgcc gaggaggtcg tgctggagcg
     7561 agcgtatact ccccctcgca acctttaagc cggtcccgtg aggccgccaa ggagcatcgt
     7621 gatcgagacc gaacccgccc tcgcgcggcg ccaccggccg gcggacgcct ggccccaccg
     7681 tcacctgctc gacgtcgacg cgctctcgcg cgcggaagtg gagcgttgcc tggatctcgg
     7741 tcgcgagatg cgccagctcc atgaacgagg cgatcggctg gatgcgctgg cgggccacgt
     7801 ggtcgggctg gcgttcgccg aggcctcgac ccgcacccgg gtcagcttcg agatcgcggc
     7861 cggtcggttg ggggcgcagg tcgtggagct gccggtcgag gccagcagcg tgaccaaggg
     7921 cgagtcgctg acggacaccc tgcgcaccct ggaacggacc ggcgtgggca ccgtggtgct
     7981 gcgccacgcc gcaagtggcg ccccgtacct ggccgcccgg gccaccgggc tgcgggtggt
     8041 gaatgccggg gacggcgcgc acgctcaccc cacccaggcg ctgctggacg cgctcaccct
     8101 ggtcgaggcg ctggatggac tcgacgggcg cacggtgacc atcgtgggtg acctcgccca
     8161 ctcgcgcgtg gcacgctcca acctccacct gctgcagctg ctcggcgcgc gcgtgcgcct
     8221 ggccgggcct cccgcctggg tcggtgcctt caggtcatgg gacggcgtgg atgtcacgac
     8281 caacctggag gaggccctgg acgggaccaa tgcggtcatg gccctccgcg tccagctgga
     8341 gcgcgacgct ggcggcggcg ttccgagcct cagggagtac gcccgccgct ggggtctgga
     8401 cgagtcgcgc atgcgccgcg ccgcgcccgg cgcgccgctc ctgcatcccg gacccaccaa
     8461 cgagggcgtc gaggtcagcg ccgacctggc cgccgggccc cgcagcctga tcggtcgcca
     8521 ggtggccaat ggggtcgccg tgcgaatggc cgtcctggct ctgctggcgc agtgacccgc
     8581 ttcggttcca tttccgaccc gccaaccgcg ctcctgatca ccggtgcacg cgtcctcgac
     8641 ccggccgccg agtcggacgc cgtccgcgac ctggctatcg ccggcggccg cctcgtggag
     8701 cctgctgatc tgccgcggga ggctgctcgc gtggacgggt ccggcctgct ggccatcccc
     8761 gcgctgtgcg atttgcattc ccacctgcgc gacccgggcg gggaggcggc cgagacgctg
     8821 gagagcgggt cgcaggcggc ggcgcacggc ggctacgcca gcgtgtgtgc catgccgaac
     8881 acggagccgg ccctggac
//
LOCUS       DATACA010000069         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2500588, whole genome shotgun sequence.
ACCESSION   DATACA010000069 DATACA010000000
VERSION     DATACA010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500588"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VHK28_01595"
     CDS             complement(<1..438)
                     /locus_tag="VHK28_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2140954.1"
                     /translation="MTYLLTSEHDELRAVVRELAEERIAPRAAAIDESGEFPMDLKEL
                     LAKQDLLGTCFEERHGGTALDTIAQSILVEELARADATTSLIPIVQKLGALPIILSGT
                     DEQKDRYLPRLASGEWLIAFALTEAAAGSDVAANKMRARLDGDC"
     gene            complement(825..1691)
                     /locus_tag="VHK28_01600"
     CDS             complement(825..1691)
                     /locus_tag="VHK28_01600"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2140955.1"
                     /translation="MIERWARPLTGLWNGLAEALRAIYRALGGVGRLLQDLLNGVWLG
                     HPLHAVIVDVVVGAATAALLLDVLRVFFGVDGLEVATTWIVALTFVSGLGAILSGLTD
                     FKDTSAGDDRNVVGLHGLINIVGALGIGLSLWLRLVDSHDSGFWAFLAGYAVISVGAY
                     IGGHVVYKFGYMVNRNAFARGKKAKEWTAVLPATSLFDNTPTKASLGATSLVVVRRGD
                     VVHALKENCSHVGGPLSEGTLEGDTIVCPWHQSAFRLTDGTVRHGPAQSRQVAYHARI
                     NGDQVEVSGPME"
     gene            complement(1695..3125)
                     /locus_tag="VHK28_01605"
     CDS             complement(1695..3125)
                     /locus_tag="VHK28_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2140956.1"
                     /translation="MTVAERQGVNRDRLREIGRREEAAYRERTARSAELHARARESMP
                     LGVASSFQTYDPYPLFMTDARGSRIWDADGNEYIDFDMAFGVLAAGHSHPLLAEALAR
                     RVANGTCYTFPVEDGILLAEEIKRRFNADLVRFSNSGTEATMDAIRVARGFTGREKII
                     KFEGGYHGHHDDVLVSIQPPREVMGPDAQPATVPATAGLPRSRLAETVVAPFNDAEKV
                     AAILEAHRGEIAAIIVEPVQFNIGVVPPKPGFLEELRRLADEHGIVLIFDEVKTGAVI
                     AWGGATEYFGVQPDLLCLANSIGGGIPIGAFAGRGDVMRVIEEVHADAPAAGIEHSTL
                     AGGATRVAHLGTFNGNPLSMLAGVVTLTQVLTRDTYPRLSAMAERLTAGCQEVLDEFE
                     IPGYALNIGPKGCVMFTPERVTNYRDFIGLDGELWQAFFFYLANRGILLPPGPDDQWT
                     LSVQHTDEEIERHIAVFRDFAVELGG"
     gene            complement(3136..3621)
                     /locus_tag="VHK28_01610"
     CDS             complement(3136..3621)
                     /locus_tag="VHK28_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2140957.1"
                     /translation="MAVRTLGSSGRRPVLDDLDKAIIKALQMDGRRPYAQIGRDLKVP
                     EATVRQRAERLISRGIVQVVGVTDPLAMGFGQPAFIGLHVDAASIDQVSEAVAALEEV
                     TYVVITAGRFDMICEVVCEDNEHLLRVLADKLATIEGVRSTETMVELRFVKESYRWGT
                     R"
     gene            complement(3715..>4239)
                     /locus_tag="VHK28_01615"
     CDS             complement(3715..>4239)
                     /locus_tag="VHK28_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2140958.1"
                     /translation="IRNEYPQFGLDGVRVVHHTELLDELVADGRLVPDQHHEAVVAYH
                     DACYLGRYNDIYEPGRRVAAAVPGQAVVEMDLHHRRGMCCGAGGARFWMEEHEGERIN
                     HRRVKQALEVVPDTIATACPFCLVMLRDGVTDLQRTDVAVSDVAELLADATGAWRVPA
                     EDGPSAAEEPPPAH"
BASE COUNT          661 a         1489 c         1431 g          658 t
ORIGIN      
        1 gcagtcgccg tctaagcggg cgcgcatctt gttggcggcc acgtcactgc cggcggcggc
       61 ctcggtcagg gcgaaggcga tcagccactc cccggaggcc agccgcggga ggtatcggtc
      121 cttctgctcg tccgtcccgg acaaaatgat gggtagcgca ccgagcttct ggacgatggg
      181 aatcaaactg gtggtcgcgt cggcgcgggc gagctcctcg accaggatcg actgcgcgat
      241 ggtgtccagc gccgtcccgc cgtgccgctc ctcgaagcag gtgcccagca gatcctgctt
      301 ggccagcagc tccttgaggt ccatggggaa ctcgccggac tcgtcgatgg ctgccgcccg
      361 cggcgcgatc cgttcctcgg cgagctcgcg caccacggcg cgcagctcat cgtgctcaga
      421 ggtgaggagg taggtcacgc ggggacggtt cctttcgtgc gtggcgcgcg agtcggccgt
      481 tcgggacgaa ggataccgaa tcgggcggca cgcctcggcg acggcccttg cagccgatcc
      541 actctcaggc gcgcccatca gtagcgttat cggttgacgg cggccgatcc actctcgggc
      601 gcgcccaccg gtcgcgttag aggggaccgg tgaccagtcg gaggccgatc cacgctcagc
      661 gcacgctcgg ccacgcgtcc atcacgaccg cttcgcggtg tgaatagttc actaacgcga
      721 cccccattcg cgcctgggaa cggggtcctc tcgagcagcc tctctaccgg cgcgactccc
      781 ggaatcggca gccagcaagc caccatgccg cgacgggggc gcggctactc catcgggccg
      841 gaaacctcca cctggtcgcc gttgatccgc gcgtggtagg cgacctgccg tgactgcgcc
      901 gggccgtggc gaacggtgcc atcggtcagg cggaaggcag actggtgcca agggcagacg
      961 atggtgtccc cctccagcgt cccctccgac agcggaccgc caacgtggga gcagttctcc
     1021 ttgagcgcgt ggaccacgtc ccctcggcgg acgaccacca gggaggtcgc tccaagcgac
     1081 gccttggtcg gcgtattgtc gaacagggag gtggcgggca gcaccgcggt ccactccttg
     1141 gccttcttgc cgcgtgcgaa cgcgttgcgg ttgaccatgt agccgaactt gtagaccacg
     1201 tggccgccga tgtaggcgcc caccgagatg accgcgtacc cggccaggaa ggcccagaac
     1261 ccggaatcat ggctgtcgac caggcgcagc cagagcgaga ggccaatgcc gagggccccg
     1321 acgatgttga tcagcccgtg cagcccgaca acattgcgat cgtcccccgc cgaggtgtcc
     1381 ttgaagtcgg tcagcccgct caggatggcg cccaggccgg agacgaaggt gagggccacg
     1441 atccaggtcg tggccacctc cagcccgtcc accccgaaga agacgcgcaa cacgtccagc
     1501 aggagggccg cggtcgccgc gccgaccacc acgtccacga tcaccgcgtg cagcggatga
     1561 cccagccaca cgccgttgag caggtcctgc agcaggcgtc caacgcctcc cagcgcgcgg
     1621 tagatggcgc gcaacgcctc cgccagcccg ttccacaggc ccgtcagcgg ccgtgcccaa
     1681 cgttcgatca tggcctaacc tcccagctcc accgcgaagt cgcggaacac tgcgatgtgg
     1741 cgctcgatct cctcgtcggt gtgctgcacc gagagggtcc attggtcgtc cgggccgggc
     1801 ggcagcagga tcccgcggtt ggccaggtag aagaagaagg cctgccacag ctcgccatcg
     1861 aggccgatga agtcgcggta gttggtaacg cgctcagggg tgaacatcac gcagcccttg
     1921 ggaccgatgt tcagcgcata gccggggatc tcgaactcgt cgagcacctc ctggcagccg
     1981 gcggtcaggc gctcggccat ggcgctcagc cgcgggtagg tatcgcgggt caggacctgg
     2041 gtcagggtca ccacgccagc cagcatggag agcgggttgc cgttgaacgt acccaggtgc
     2101 gcaacgcgag tcgcgccacc agccagcgtg gagtgctcga tgccggcggc cggagcatcg
     2161 gcgtggacct cctcgatgac ccgcatcacg tcgccccttc cggcgaaggc gccgatcggg
     2221 atgccgccgc cgatgctgtt ggccaggcac agcaggtccg gctgcacgcc gaagtactcg
     2281 gtggcgccgc cccaggcgat caccgcgccg gtcttgacct cgtcgaagat gagcacgatg
     2341 ccgtgctcgt cggcgaggcg gcgcagctcc tccagaaagc cgggcttggg cgggaccacc
     2401 ccgatgttga actggaccgg ctcgacgatg atggccgcga tctcgccgcg atgggcctcc
     2461 aggatggcgg cgaccttctc cgcgtcgttg aacggggcga ccaccgtctc ggccaggcgg
     2521 ctgcgcggca ggccggccgt ggccggcacg gtagcgggct gcgcgtccgg acccatcacc
     2581 tcgcgcggcg gctggatgct gaccagcacg tcgtcgtggt ggccgtggta gccaccttcg
     2641 aacttgatga tcttctccct cccggtgaag ccgcgcgcca cccggatggc gtccatggtc
     2701 gcctcggtgc ccgagttgct gaagcgcacc aggtcggcgt tgaagcgccg cttgatctcc
     2761 tcggcgagca ggatgccgtc ctcgaccggg aaggtgtagc aggtgccgtt ggccacccga
     2821 cgggccagcg cctcggcgag cagtggatgg gagtgccccg ccgccagtac cccgaatgcc
     2881 atgtcgaagt cgatgtactc gttgccgtcc gcgtcccaga tccgggagcc acgggcatcg
     2941 gtcatgaaca gcgggtacgg atcgtaggtc tggaaggagc tggccacgcc cagcggcatg
     3001 gactctcgcg cgcgggcgtg cagctcagcg ctgcgtgccg ttcgctcccg gtaggcggcc
     3061 tcctcgcgcc ggccgatctc cctgagccgg tcgcggttga cgccctgtcg ctcggctacg
     3121 gtcacctggg tcctcctacc tcgtgcccca ccggtagctc tccttgacga accgcagctc
     3181 caccatcgtc tcggtggagc gtactccctc gatcgtggcc agcttgtcgg ccagcacccg
     3241 cagcaggtgc tcgttgtcct cgcagaccac ctcgcagatc atgtcgaagc gtccggcggt
     3301 gatgaccacg taggtgacct cttcaagcgc cgccaccgcc tccgacacct ggtcgatgga
     3361 cgccgcgtcc acgtgcagac cgatgaatgc cggctgcccg aagcccatgg ccagcggatc
     3421 ggtgaccccg accacctgca cgattccacg ggagatgagt cgctccgccc gctggcggac
     3481 ggtcgcctcc ggcaccttca ggtcccggcc gatctgcgcg tacgggcgcc ggccgtccat
     3541 ctgcagggcc ttgatgatcg ccttgtccag gtcgtccagc accgggcgac gaccggacga
     3601 gccgagcgtt cgcactgcca tcctgataca gaatcgtagt cctggcctgc atggtggtca
     3661 acattccgca acccgggcag ccgaagttcg cggaatccgc gtagtcctgc gggatcagtg
     3721 ggccggaggt ggctcctccg cggcggacgg tccgtcctca gccggcaccc gccaggcgcc
     3781 ggtggcgtcc gccagcagct cggccacgtc ggagactgcc acatcggtcc gttgcaggtc
     3841 ggtcaccccg tcacggagca tgaccagaca gaaggggcac gcggtggcga tggtgtccgg
     3901 caccacctcc agcgcctgct tgacgcgtcg gtggttgatg cgctcgccct cgtgctcctc
     3961 catccagaag cgggcgccgc cggcgccgca gcacatgccg cggcggtggt ggaggtccat
     4021 ctcgacgacc gcctggccgg ggaccgcggc ggcgacccgg cgcccgggct cgtagatgtc
     4081 gttgtaacgg cccaggtagc aggcgtcgtg gtaggcaacc accgcctcgt ggtgctggtc
     4141 cggcaccagc cggccatcgg ccaccagctc gtcgagcagc tcggtgtggt gcacgacccg
     4201 caccccgtcc agcccgaact gcgggtactc gttgcggat
//
LOCUS       DATACA010000070         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1055282, whole genome shotgun sequence.
ACCESSION   DATACA010000070 DATACA010000000
VERSION     DATACA010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055282"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VHK28_01620"
     CDS             <1..814
                     /locus_tag="VHK28_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140959.1"
                     /translation="LELAESDADRARAWSALAGIPYRHGDMHAALAAYRSGLASLSGS
                     DTRAHARLESDSAWALYRLGRHDEALKVLERVASVLVEAPETHLRCRTKDRLGLVYHA
                     VGRAEEGLAWLDDGVADATEQDDDRELAVLCLHRGGLLVQLGRHAEAAADLARAATIA
                     DAAHDRYLRSVIRWNTADLHEARGDLAAALAERDAEVTQLREIGNDRNLAGAQRHRAR
                     LLSKLGRQAQAVQAAREARQAAARTGDADLVGGIGQELERILAGKGSGTATS"
     gene            868..1284
                     /locus_tag="VHK28_01625"
     CDS             868..1284
                     /locus_tag="VHK28_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX2140960.1"
                     /translation="MSATETLRVVQGYLDGHAVEWLSEDVEFRDMTRPEPMRGRREVG
                     AFLHLFFAQVFSEARIDQVRLTPADHRVCVEWTFRARHSGSLAGETPTGRDVVHPMAC
                     VYEVVGGEIRRASLYYDGAGLLAQLGIGSAPATVAV"
     gene            1316..1672
                     /locus_tag="VHK28_01630"
     CDS             1316..1672
                     /locus_tag="VHK28_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140961.1"
                     /translation="MKKLFMLATLLLVLAFPMGVAANGPTVDKLINAGWLCDDPDGPA
                     GPLEIHCYTQANNRQAVSAAVSAMVFDPVTGAFLATEVLRFTGKDLSGIPCPTGHEGY
                     WEDLGFAWACHHWKPF"
     gene            complement(1915..>2128)
                     /locus_tag="VHK28_01635"
     CDS             complement(1915..>2128)
                     /locus_tag="VHK28_01635"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2140962.1"
                     /translation="AEAQRLLARWLFDNSDLNRVEASTDVENVAEQRSLEKAGFVREG
                     IQRGAQFRAGAYHDLVTYAVIRSDLG"
BASE COUNT          332 a          720 c          760 g          316 t
ORIGIN      
        1 cctcgagctc gccgagtcgg atgcggatcg agcacgcgca tggagcgcgc tggcggggat
       61 tccgtaccgg cacggcgaca tgcacgcagc gctggcggcg tatcggtccg ggctggcgtc
      121 gctctcgggc tcggataccc gagcgcacgc gcgcctggag agtgattcgg cctgggcgct
      181 gtaccggctg ggccgtcatg acgaggcgct gaaggtcctg gaacgggtgg catcggtgct
      241 ggtcgaggca cccgagactc atctgcgctg ccgcaccaag gaccggctgg ggctggtgta
      301 ccacgccgtt ggccgcgccg aggagggact ggcctggctg gatgacggcg tggcggatgc
      361 caccgagcag gacgacgatc gagagctggc cgtgctgtgc ctgcatcgcg ggggcctgct
      421 ggtgcagctc ggtcggcatg cggaggcggc ggccgacctg gctcgggcgg cgaccatcgc
      481 ggacgccgcg cacgatcggt acctgcggtc ggtcatccgc tggaacaccg ccgacctcca
      541 cgaggcgcgt ggtgacctgg ctgccgcgct ggcggaacgc gacgcggagg tgacgcagct
      601 gcgcgagatc ggcaacgacc gcaacctggc tggtgcgcaa cgccaccgcg cgcggctgct
      661 gtcgaaattg ggacgccagg cacaggcagt acaggccgcg cgggaggcca ggcaggcggc
      721 ggcgcgtacc ggtgacgcgg acctggtagg cggaatcggt caggaactgg agcggatcct
      781 ggccgggaag ggttcgggaa cggccacttc ctagcctcct ttacaggctg cgcaccaagc
      841 gcaccaagtc gaggaggtcc ggtagagatg tccgccacag agacgctgcg cgtggtgcag
      901 gggtacctcg atggccacgc cgtggagtgg ctgtccgagg acgtcgagtt ccgcgacatg
      961 acgcgccctg aaccgatgcg ggggcgccgc gaggtcggcg cattcctgca cctgttcttc
     1021 gcgcaggtct tcagcgaggc acgcatcgac caggtgcgac tcacccccgc cgaccaccgc
     1081 gtctgcgtgg aatggacctt ccgtgcccgc cattccggat cgctggccgg cgagacgccg
     1141 accggtcgag acgtggtgca tccgatggcc tgcgtgtacg aggtggtggg cggcgagatc
     1201 cgccgcgcca gcctctacta cgacggcgcc gggctccttg cccagctcgg tatcggctcc
     1261 gcaccggcga ccgtggcggt ctgaccaggc ccgctcagca cacgaaggag aacccgtgaa
     1321 gaaactgttc atgctcgcga ccctgctgct cgtgctcgcc tttccgatgg gcgtcgcggc
     1381 gaacggcccc accgtcgaca agctgatcaa cgccggttgg ctgtgcgacg accctgacgg
     1441 cccggcaggt ccactcgaga tccactgcta cacgcaggcc aacaaccgcc aggccgtctc
     1501 ggccgcggtc agcgcaatgg tcttcgaccc ggtcaccggc gcattcctgg ccaccgaggt
     1561 cctgcgcttc accggcaagg acctgtccgg catcccctgc ccgacgggtc acgagggcta
     1621 ctgggaggac ctgggcttcg cctgggcctg ccaccactgg aagcccttct agggaccgat
     1681 gagcaggtgc cccggccgcg ccggggcacc tgcacctgag ccgggacgtg gttctgcccg
     1741 atctatcgcg gcgctgagca caggaagggt tcgagccggg tcacctgact catctatcgc
     1801 ggcaaggagc acgggaaggc acggaaatcg cccgttcctg cgcgccctgc cctcatagat
     1861 cggtcaggcg gcacgccgga gcggcttcgc gcgctaaatg caccgataga tcgctcagcc
     1921 gaggtcggag cggatcaccg cgtaggtcac caggtcgtgg taggcccccg cgcgaaactg
     1981 tgcgccgcgc tggatcccct cccgcacgaa cccggccttc tccagtgacc gctgctcggc
     2041 gacgttctcc acgtcggtcg aggcctcaac gcggttcagg tccgagttgt cgaacagcca
     2101 gcgggcgagc aggcgttggg cctcggcg
//
LOCUS       DATACA010000071         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_171777, whole genome shotgun sequence.
ACCESSION   DATACA010000071 DATACA010000000
VERSION     DATACA010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171777"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VHK28_01640"
     CDS             <1..303
                     /locus_tag="VHK28_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140963.1"
                     /translation="RELVVQGVRQVAATADVVIASRAMGKGKTFATLVGMGLLLLGFD
                     AATGGPLSGLGGVGAMLQTAGYWVMVVATALSVASGWDYVRGALPVLLGHPDEPPT"
     gene            complement(296..1120)
                     /locus_tag="VHK28_01645"
     CDS             complement(296..1120)
                     /locus_tag="VHK28_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018554504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2140964.1"
                     /translation="MTAVPRRLPAPAGAPAVLGTLRRARRVVQRNMMVYRHSWIVIVS
                     GFFEPLFYLLGIGFGLGTFVQTVEVRPGLEIPYAAFVAPGLLAMSCLNGAIAETIFNV
                     FFRLNYERIYEGMMATPLGIRDIAIGELIWSLFRGTLYAAAFVGVMFMLGLVFSPWVL
                     LVVPGAILLGAAFSAAGLATTSFLRTVQDFDLPMGLVVMPMFLFGGVFFPIAVYPEPV
                     QILVQALPIYRGVHLFRGLSTGTLDAWMLFDVAYLLVLTAIGLTIAMRQMERRLIK"
     gene            complement(1131..1919)
                     /locus_tag="VHK28_01650"
     CDS             complement(1131..1919)
                     /locus_tag="VHK28_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2140965.1"
                     /translation="MTVSTLAPAARVLQRNLLVYRRTWHGSILGSFLTPLLYLTAMGI
                     GLGTLVQARGTDAFAGYDYVHFLGPGILAATTMQSAVFESTFPVLAKIAWRRNYEAIL
                     ATPLGIRHLVGGELLWVGFRVLTISSVFLVVLTLFGIPRSPLALLAIPSAMLTGIAFS
                     SVVIAFSSTQRTGEGFAWMFRFIVNPLFLFSGTFFPLSQLPEPVQWAAALTPLYHGVA
                     LVRGTILAEPVFLGPWALHVAYLLGMLAMGSYLAHRLLKRRLLS"
     gene            complement(1925..>2317)
                     /locus_tag="VHK28_01655"
     CDS             complement(1925..>2317)
                     /locus_tag="VHK28_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2140966.1"
                     /translation="LYRLKQRGRTLIITTHYMDEAEQLCDRLVVMDKAKIVAEGSPRQ
                     LIERYSTREVLELRLADAVRASLNGRLDGIADRIEELPDRMLLYVADGERALEVVHDR
                     QVALESALVRRATLEDVFLRLTGRTLVE"
BASE COUNT          414 a          765 c          833 g          305 t
ORIGIN      
        1 cgcgagctcg tggtccaggg cgtgcgccag gtggctgcca ccgccgacgt ggtgatcgca
       61 tcgcgcgcca tgggcaaggg gaagacgttc gccacgctgg tgggcatggg actgctgctg
      121 cttggcttcg acgcggcaac cggcggcccg ctctccgggc tcggtggcgt gggagccatg
      181 ctgcagaccg ccggctactg ggtgatggtc gtggccacgg cactcagcgt ggcctccggc
      241 tgggattacg tccgcggcgc gctgccggtg ctgctcggcc acccggacga gccgcctact
      301 tgatcagtcg ccgctccatc tgccgcatgg cgatggtgag cccgatggcg gtcagcacca
      361 gcaggtaggc gacgtcgaac agcatccagg cgtcgagagt ccccgtcgac aggccgcgga
      421 acaggtgcac gccgcggtag atgggcagcg cctggacgag gatctgcacg ggctccgggt
      481 agacggcgat cgggaagaag acgcctccga acaggaacat gggcatcacc accaggccca
      541 tgggcaggtc gaagtcctgc acggtgcgca ggaaggaggt ggtcgccagc ccggccgcgc
      601 tgaaggcggc gcccagcagg atggcgcccg gcaccaccag cagcacccac ggcgagaaca
      661 ccaggccgag catgaacatc acgcccacga aggctgcggc gtacagcgtc ccacggaaca
      721 gcgaccagat cagctcgccg atggcgatat cccggatgcc gagcggggtg gccatcatgc
      781 cctcgtagat gcgctcgtag ttgaggcgga agaagacgtt gaagatggtc tcggcgatcg
      841 ccccgttcag gcacgacatg gccagcagcc cgggtgccac gaaggcggcg taggggatct
      901 ccaggcccgg acggacctcc acggtctgca cgaaggtgcc gagcccgaag ccgatgccca
      961 gcaggtagaa cagcggctcg aagaagcccg agacgatgac gatccagctg tgccggtaga
     1021 ccatcatgtt gcgctgcacc acccggcggg cgcgccgcag cgtgccgagc acggctggtg
     1081 cgccagcggg tgccgggagg cggcggggga cggcggtcat cggtccatcc tcagctcagc
     1141 aggcggcgct tcagcagccg atgcgccagg tagctgccca tcgccagcat gccgagcagg
     1201 taggccacgt ggagcgccca cggccccagg aagacgggtt cggccaagat cgtgccgcgc
     1261 accagggcta ccccgtggta cagcggcgtc agggccgccg cccactggac cggttccggc
     1321 agctggctca gcgggaagaa ggtaccgctg aacaggaaca gcgggttgac gatgaagcgg
     1381 aacatccagg cgaacccctc gccggtccgc tgcgtggacg agaaggcgat gaccaccgag
     1441 ctgaatgcga tcccggtcag catggcggac gggatggcca gcagcgcgag cggggagcga
     1501 gggatgccga acagggtgag caccaccagg aacacggacg agatggtgag cacccggaag
     1561 ccgacccaca gcagctcgcc gcccaccagg tgccggatgc cgagcggagt ggcaaggatg
     1621 gcctcgtagt tccggcgcca tgcgatcttg gccaggaccg ggaacgtcga ctcgaagacg
     1681 gccgactgca tggtggtggc cgccaggatg cccggcccca ggaagtggac gtagtcgtat
     1741 ccggcgaagg cgtccgtgcc gcgggcctgg accagcgtcc ccagcccgat gcccatggcg
     1801 gtcaggtaca gcagcggcgt caggaacgag cccagtatgc tgccgtgcca ggtgcggcgg
     1861 tagacgagca ggttgcgctg cagtacgcgc gcggccgggg ccagggtgct gacggtcatg
     1921 ctgcctattc gaccagcgtg cgcccggtca ggcgcaggaa gacgtcctcc agcgtggcgc
     1981 gccgcaccag tgccgactcc agtgcgacct ggcggtcgtg gaccacctcc agcgcccgct
     2041 cgccgtcggc cacgtacagc agcatccggt ccggcagctc ctcgatccga tccgcgatcc
     2101 cgtccaggcg cccattgagc gaggcgcgca cggcgtccgc cagccgcagc tcgagcactt
     2161 cgcgcgtcga gtagcgctcg atcagctgac ggggcgagcc ttcggccacg atctttgcct
     2221 tgtccatgac caccagccgg tcacagagct gctccgcctc atccatgtag tgggtggtga
     2281 tgatcagcgt ccgtccgcgc tgcttgaggc ggtagag
//
LOCUS       DATACA010000072         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1295838, whole genome shotgun sequence.
ACCESSION   DATACA010000072 DATACA010000000
VERSION     DATACA010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295838"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHK28_01660"
     CDS             <1..198
                     /locus_tag="VHK28_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140967.1"
                     /translation="TAQQIGGFVVIPIIAVAVAQFFGGQPTFTMQQVLIGDLVVAALI
                     GAAMVLGSWAFERESILSRLA"
     gene            complement(531..803)
                     /locus_tag="VHK28_01665"
     CDS             complement(531..803)
                     /locus_tag="VHK28_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140968.1"
                     /translation="MTSDDRDITQPTEHERRVADELEERTGESEGPLGHDDVLTYGDQ
                     TDEQAGGTDRPTYDEEVEAERAGTTAEEVREDDDSALDSAYKPRTG"
     gene            complement(893..1795)
                     /locus_tag="VHK28_01670"
     CDS             complement(893..1795)
                     /locus_tag="VHK28_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2140969.1"
                     /translation="MTAINGAQPQAARERTGVAAALASLIDRWPLHLVLVIIGLLWVL
                     PTVALLSSSFRPANEVLTTGWWNAFTLPFDFTLQNYQDVLDRNNIGQSFINSLFVSIP
                     ATLIPIMVAAFAAYAFAWMDFPGRNALFILVVGLLVIPLQSALVPVLQFLTDMGVTGS
                     FLGIWLAHTGFGLPFGIFLLRNFIGALPREMFESAFIDGASPVTAFFRLVMPLSVPAI
                     ASFAIFQFMWVWNDLLVALIILGGTPDVAPLPVQISSLVSSLGQGWHLLTAAAFISMM
                     LPLVVFFALQRYFVRGILGGSVKG"
BASE COUNT          334 a          606 c          645 g          277 t
ORIGIN      
        1 accgcccagc agatcggcgg gttcgtggtg atccccatca tcgcggtggc ggtcgcgcag
       61 ttcttcgggg ggcagccgac gttcaccatg cagcaggtgc tgatcggcga cctggtggtg
      121 gccgccctga tcggcgccgc catggtgctc ggatcatggg ccttcgagcg ggagtccatc
      181 ctcagccgcc tggcgtagcc ttgacccgat ggctggcctg ttgtgcgtcg gcccgatcct
      241 ctcagacgcg accaccagtc gcgctatccg gctcggatgg gctaaccccc ctcaggcgcg
      301 accacccgtc gcgttggaga tcatctcgaa cctttcggcg tgcctcgcga cagcgttcca
      361 cgctcacgga tggtcgctgg aacgacgaaa taagcctgag gacttctcta acgcgatccc
      421 caggcgcgcc tgagaagagg ccggagtgag gagttctcta acgcgattcc caggcgcgca
      481 agagagaagg caggtgtgac gacgtcttcc tgcgcgcctg agagaaggcg ctatccggtt
      541 cgaggcttgt acgcggagtc cagggcggag tcgtcgtcct cgcggacctc ttcggccgtg
      601 gtcccggcgc gctccgcctc gacctcctcg tcgtaggtcg gccggtccgt gccgccggcc
      661 tgctcatcgg tctggtcgcc gtaggtcaac acgtcgtcgt gcccaagggg gccctcggac
      721 tcgccggtgc gctcctccag ctcgtcggcg acacgccgtt cgtgctccgt cggctgcgtg
      781 atgtcgcggt cgtcactcgt catcgaaaaa cctcccgggc cggtgggttc gtatccggcc
      841 cacgaacccg ggaggtgcac gcagcgcgcc agcgagccgc cagcgagccc ggtcagccct
      901 tgacggaccc gcccaggatg ccgcgcacga agtagcgctg gagggcgaag aagacgacca
      961 gcggcagcat catgctgatg aaggccgcgg cggtcagcag gtgccagccc tggcccagcg
     1021 agctgaccag cgacgagatc tgcaccggca gcggcgccac gtccggcgtt ccgcccagga
     1081 tgatgagggc caccagcagg tcgttccaga cccacatgaa ctggaagatg gcgaacgacg
     1141 cgatggcggg caccgacagc ggcatcacca gccggaagaa ggcggtcacc gggctggcgc
     1201 cgtcgatgaa cgccgactcg aacatctcgc gcggcagcgc cccgatgaag ttgcgcagca
     1261 ggaagatgcc gaacggcaaa ccgaagccgg tgtgggccag ccagatgcca aggaacgagc
     1321 cggtgacgcc catatccgtc aggaactgga gcaccggcac cagcgcggac tgcagcggga
     1381 tgaccagcag gccgaccacc aggatgaaca gggcgttgcg tcccgggaag tccatccagg
     1441 cgaaggcgta agcggcgaag gccgcgacca tgatggggat cagcgtggcc gggatggaca
     1501 cgaacaggct gttgatgaag ctctgcccaa tgttgttgcg gtccagcacg tcctggtagt
     1561 tctgcagcgt gaagtcgaac ggcagggtga acgcgttcca ccagccggtg gtcagcacct
     1621 cgttcgccgg ccggaaggag ctggatagca aggccacggt gggcagcacc cagagcaggc
     1681 cgatgatgac cagcaccagg tgcagcggcc accgatcgat caggctggcg agggcggccg
     1741 cgacaccggt gcgctcgcgg gcggcctggg gctgagcgcc gttgatcgcc gtcatcgaat
     1801 cgcctcctgc gcctggaacc ggcggatgtt gaagagcatg acgggcacga tggcgagcag
     1861 ca
//
LOCUS       DATACA010000073         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_171872, whole genome shotgun sequence.
ACCESSION   DATACA010000073 DATACA010000000
VERSION     DATACA010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171872"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1418
                     /locus_tag="VHK28_01675"
     CDS             162..1418
                     /locus_tag="VHK28_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2140970.1"
                     /translation="MTAPSSIDPARFLHEHLASASPDLLRSMLTTFINTLMSAEADAV
                     CGAGYGETSPDRTNVRNGYRHREFDTRAGTLDVAIPKLRSGSYFPDWLLERRRRAERA
                     LTTVVATCYLLGVSTRRMEKLVESLGITRLSKSQVSTMAAELDAHVADFRTRPLDQGP
                     YTFVAADALVLKVREGGRVVNVHALLATGVNADGHREILGLQVTSAEDGAGWLGFFRD
                     LTARGLAGVRLVTSDAHRGLVEAIGASLPGASWQRCRTHYAANLMAATPKTSWPWVRA
                     LLHSVYDQPDAGSVHAQFDRVLDAVADKLPKVAEHLDTARADVLAFTGFPKEVWRQIW
                     SNNPSERLNREIRRRTDVVGIFPDRDALIRLVGAVLAEQHDEWAEGRRYLGLDVLARS
                     RIAAVPDATTSSEEVTTTDTIPALSA"
BASE COUNT          250 a          606 c          501 g          254 t
ORIGIN      
        1 cccttggtca cgggatcgcg gcggtgtgct gcttcagtcg gtagcggcgt ccgccaaggt
       61 ggtgtgcgtc cggcccggtg acgggcgcgc cgaggtgtag ggcggccatc gcgtgatctt
      121 cttgaagacc tctcacagtg cttctcgaag gacctcacgc gatgaccgca ccgagcagta
      181 tcgaccctgc ccgtttcctg cacgagcacc tggcctccgc ttcgccggac ctgctgcgct
      241 cgatgctgac caccttcatc aacaccctga tgtccgccga ggccgacgcc gtctgcggcg
      301 ccggctacgg cgagaccagc ccagaccgga ccaacgtgcg caacggctac cggcaccgcg
      361 agttcgacac ccgcgccggc accctcgacg tcgcaatccc caagctgcgt tcgggctcct
      421 acttccccga ctggctgctc gaacgccgcc gccgcgccga acgcgccctt accacggtgg
      481 tcgccacctg ctacctgctc ggcgtctcca cacgacggat ggagaaactg gtcgaatcct
      541 tgggaatcac caggctgtcc aagtcccagg tctcaaccat ggccgccgag ctcgacgccc
      601 atgtcgcgga cttccgcacc cgcccgctcg atcaaggccc gtacaccttc gtcgccgctg
      661 acgccctggt gctcaaggtc cgtgagggtg ggcgggtggt caacgtgcac gctctgctcg
      721 ccaccggcgt caacgccgac gggcaccggg aaatcctggg cctgcaggtc acgagcgccg
      781 aggacggcgc tggctggctg gggttcttcc gggacctgac cgcccgcggt ctggcggggg
      841 tccggctcgt cacctccgac gcgcaccgcg ggctggtcga ggcgatcggc gcgtccctgc
      901 ccggtgcgtc ctggcaacgc tgcagaaccc actacgccgc gaacctgatg gccgccaccc
      961 cgaagacgtc ctggccctgg gttcgtgcgt tgctgcactc ggtctatgac cagcccgacg
     1021 ccgggtcggt gcacgcccag ttcgaccggg ttctcgacgc cgtggccgac aagcttccga
     1081 aggtggccga gcacctcgac accgcccgcg ccgacgtgct ggcgttcacc ggattcccga
     1141 aggaagtctg gcgccagatc tggtcgaaca acccctccga gcggctcaac cgggagatcc
     1201 gccgccgcac cgacgtcgtc ggcatcttcc ccgaccgcga cgccctgatc cgcctcgtcg
     1261 gcgccgtgct cgctgaacag cacgacgagt gggccgaagg gcggcgctac ctcggcctcg
     1321 acgtcctggc ccgctcccga atcgccgccg ttcccgacgc cacaaccagc tccgaggagg
     1381 tgaccacgac cgacaccatc cctgcgctca gcgcctgaca agcaggaaga tcacgcatcg
     1441 accgtcaaac accacgccag cggacttgac cggccatgac cccgccgggc ttgaggtggg
     1501 cagttccgga gttgtgctgt cgggcatcgg gtcgcgtccg cagaagccca ccgggtgacg
     1561 gtcccatgtc ggtccgacag aactgtccgt cgccagttga cctgatccat t
//
LOCUS       DATACA010000074         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2260010, whole genome shotgun sequence.
ACCESSION   DATACA010000074 DATACA010000000
VERSION     DATACA010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260010"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1027)
                     /locus_tag="VHK28_01680"
     CDS             complement(224..1027)
                     /locus_tag="VHK28_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2140971.1"
                     /translation="MSYPAGQPLPHHLGEVIVTARGIEKYFGDAHILRGIDLDVHRRE
                     AVMVIGRSGSGKTTLLRCLNFLEEPTIGWVEIDGLRVEADPLHARSRAHLEQIRQLRL
                     RAGMLFQDFALFPHMSVMDNCIEAPVRVRGIPHGEALATAEKYLDKVGLLERRDEYPA
                     RLSGGQKQRVAIARALCMEPKVLLFDEPTSALDLELIGEVLKVMEELAHEGVTMIVVT
                     HEMHFAREAADRVVFMEDGVIVEQGPPEQVLNAPQDERTRGFLRRFLEM"
     gene            complement(1024..1830)
                     /locus_tag="VHK28_01685"
     CDS             complement(1024..1830)
                     /locus_tag="VHK28_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX2140972.1"
                     /translation="MIDSAAQPQPELLARIRRAQTVARRWFRLQFVLVWMAIIVALVG
                     MFAATGNVDVAWIQEWAPFILGGAGITILVCLSAIVLATVLALVGAIGRLSQRPIPHA
                     LASFYVSLVRGTPLIVQIYIIYFSLPSLGIILDAIPAGIIALGFNYGAYMTEIFRAGI
                     QAVPRNQTEAAQALGMPGPMIFRRIQLPQAVRIVTPAIGNEFIAMIKDSALVSIIGVN
                     EVLWRAETSGRPRLNTLEAFIIAAGIYWGLTIIFSFFQERLERRMARGDR"
     gene            complement(1926..2762)
                     /locus_tag="VHK28_01690"
     CDS             complement(1926..2762)
                     /locus_tag="VHK28_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX2140973.1"
                     /translation="MAARRIAPVLIGLLVFATSCQLGGGGDLLSTIESRGRIVVATDP
                     AYPPQSEQLPDGTFEGFDIDVANEIGERMGVDVEFATPNFDEVVAGNWAGRFDISVGS
                     VTITEERQAVLDFTEAYYFTPAQFGATEESGITDIEGIAGTTVCVGAATTYQSWLEGS
                     LTLVGSPEPDEPPENVEVTTFDTDTECADAIVAGRTDFEVWLTASETLKVAIDEGAPF
                     VPVGDPVFFEALAVAVDKEGPAHAELLEELDRIIAEMHEDGTLTELSQKWYDGQDLTK
                     AE"
BASE COUNT          473 a          970 c          949 g          492 t
ORIGIN      
        1 ctgacgtccg cgggtgccgt tcggttgcat tcccggcgag cagtcgagac caagccgtct
       61 cgccaggttc agatggggtg cgcaccactg tgttgaacgg tggtcggcac cgccggatgc
      121 ggccctgatc cagcgcggtg gcagtgcgag cgccctcagt cggcttgcgc tcgcacctca
      181 cctgaatctg gcgacaagga cccgccgcgc cgcggcggcg ggcctacatc tctaggaaac
      241 ggcgcaggaa gccgcgtgtg cgctcgtcct gcggggcgtt gaggacctgc tccggcggac
      301 cctgctccac gatcacgccg tcctccatga agacgacgcg atcggccgcc tcgcgggcga
      361 agtgcatctc gtgcgtcacc acgatcatgg tcacgccctc gtgcgccagc tcctccatca
      421 ccttcagcac ctcgccgatc agctccaagt ccagcgcgct ggtgggctcg tcgaagagca
      481 gcaccttggg ctccatgcac agagcacggg cgatggcgac ccgctgcttc tgtccgccgg
      541 acagcctcgc cgggtattcg tctcgacgct cgagcaggcc gaccttgtcg aggtacttct
      601 ccgcggtcgc cagtgcctcc ccgtgaggga tacccctcac tcgcacaggc gcctcgatgc
      661 agttgtccat caccgacatg tgcgggaaga gcgcgaaatc ctggaagagc atgccggcac
      721 ggagccgcag ctggcggatc tgctccaggt gcgcgcgcga gcgagcgtgc agtggatcag
      781 cctcgaccct caggccgtcg atctcgaccc agccgatggt cggctcctcg aggaagttga
      841 ggcatcggag gagcgtggtc ttgccgctcc ccgaccgacc gatgaccatc accgcctcgc
      901 ggcggtgcac gtcgaggtcg atgcctcgca ggatgtgggc gtccccgaag tacttctcga
      961 tgccacgcgc ggtcacgatg acttcgccca ggtggtgcgg cagcggctgg cctgcggggt
     1021 agctcatcgg tcaccacggg ccatgcggcg ctccagtcgt tcctggaaga agctgaagat
     1081 gatggtcagg ccccagtaga tgccggcggc gatgatgaag gcctccaggg tgttgagcct
     1141 gggccggccc gaggtctcgg cccgccacag gacctcgttc acaccgatga tggagaccag
     1201 ggcgctgtcc ttgatcatcg cgatgaactc gttgccgatg gccggagtca cgatgcggac
     1261 ggcttggggg agctggatgc ggcggaagat catcggtccc ggcatgccga gcgcctgtgc
     1321 cgcctccgtc tggttcctcg gcacggcctg gatgccagcc cgaaagatct cggtcatgta
     1381 ggccccgtag ttgaacccga gggcgatgat ccccgccggt atcgcgtcca ggatgatccc
     1441 cagtgatggc agcgagaagt agatgatgta gatctggacg atgagcggtg tcccgcgtac
     1501 gagcgagacg tagaaggacg ccagggcgtg ggggatcggt cgctgcgaga gtcgcccgat
     1561 ggcgccgacc agcgccagga cggtcgccag cacgatggcg ctgaggcaga ccaggatggt
     1621 gatcccggca ccgcccagga tgaagggcgc ccactcctgg atccaggcga cgtccacgtt
     1681 cccggtggcc gcgaacatcc ccaccagcgc cacgatgatg gccatccaga ccagcacgaa
     1741 ctgcaaccgg aaccaacgcc gggctaccgt ctgcgctcgt cggattcgcg cgaggagctc
     1801 aggctggggt tgggccgcgc tgtcgatcat cggtgctcct cctgttcacg tctgccagga
     1861 aacagaacgg ggacgccgcc cggcgtcccc gttgagcgac ccggtcctcc cgggtggtgc
     1921 agggcctact cagccttggt cagatcctgc ccgtcgtacc acttctggct cagctcggtc
     1981 agcgtcccgt cctcgtgcat ctcggcgatg atccgatcaa gctcctccag gagctccgcg
     2041 tgggccggcc cctccttgtc gacggccacc gcaagcgcct cgaagaagac cggatctccc
     2101 accgggacga agggcgcacc ctcgtcgatg gccaccttga gggtctctga ggccgtcagc
     2161 cacacttcga agtccgttcg tccagcgacg atggcgtccg cgcactcggt atcggtgtcg
     2221 aaggtggtga cctcgacgtt ctctgggggc tcgtcgggct cgggcgagcc gaccagcgtg
     2281 aggctgccct ccagccagct ctggtaggtg gtcgctgcgc ccacgcagac cgtggtcccg
     2341 gcgattccct cgatgtcggt gattcccgac tcctcggtcg cgccgaactg ggcgggcgtg
     2401 aagtagtagg cctcggtgaa gtcgagtacg gcctgccgct cttcggtgat cgtcaccgaa
     2461 ccgacgctga tgtcgaatct gccggcccag ttccccgcca ccacctcgtc gaagttcggg
     2521 gtcgcgaact cgacgtcgac gcccatccgc tcgccgatct cgttggccac atcgatgtcg
     2581 aagccctcga aggtgccgtc cggcagctgc tccgactgcg gtgggtaggc gggatccgtc
     2641 gccaccacga tgcgtccacg cgattcgatg gtgctcagca ggtccccacc accgccaagc
     2701 tggcaggagg tggcgaatac gagtagaccg atgagaactg gtgcaatgcg gcgcgccgcc
     2761 atagaactac tcctcgtgcg gggagacgaa tcctgcgctc tggtgaatgc cgtgttgtag
     2821 cacgcctgcc tcgaagccga caaggaaatc cggcgtccaa ccgcttgccc gcatggcgtt
     2881 cagc
//
LOCUS       DATACA010000075         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_412435, whole genome shotgun sequence.
ACCESSION   DATACA010000075 DATACA010000000
VERSION     DATACA010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412435"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VHK28_01695"
     CDS             complement(<1..287)
                     /locus_tag="VHK28_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2140974.1"
                     /translation="MEPTVTIRPVRPDEREAWIAASTAGYAEAVQQQGGQTPEAARAK
                     ADADVASALGRPGSTLYVVEADGSPVGRLWLAERESGGRRVVFVYDISIDP"
     gene            567..1442
                     /locus_tag="VHK28_01700"
     CDS             567..1442
                     /locus_tag="VHK28_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2140975.1"
                     /translation="MSIDRVYVVGAGLMGHGIAQVSAAAGKQVTLADRTAELADKGRQ
                     RIAGNLGRQVEKGKLEQSDAEATLARISTAEGTDEVAGHGLVVEAVFEDEPLKRETWA
                     AISERADPDTIFASNTSSISITALATATDRPERFIGLHFFSPVPVMKLIEIIRGLETD
                     DTTHAAARTFAEDVGKTVIESKDMPGFLVNRMLAPLINEAIFAYAEGTGSAEDIDTGA
                     KLGLNHPMGPLELSDFIGNDVMLGVMEVLYRGFGDPKFRAAPLLRQMVTAGHLGRKSG
                     RGFYRYDEKGNRIAG"
     gene            1439..>2122
                     /locus_tag="VHK28_01705"
     CDS             1439..>2122
                     /locus_tag="VHK28_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2140976.1"
                     /translation="MTTTTAPRQDPFALTEEERSVRDTARAFAERELAPTVAQRDEEE
                     RYDRALFDRMGELGLTAIPYPEEYGGAGMGSFAWVLGCEEIAAADMGMAVSLSVHVLA
                     QLCIHSFGTDEQKQRLLPAMTAGRHLGAFALTEPGAGSDASAISLRADRTDDGYALTG
                     TKVWITNGADAEVAIVFATVDRSRGRDGVTAFVVERGTPGFRVGGHERKMGIRDSASV
                     ELVFEEAAVP"
BASE COUNT          336 a          754 c          743 g          289 t
ORIGIN      
        1 tgcgggtcga tcgagatgtc gtacacgaac accacgcgcc gcccgcccga ctcgcgctcc
       61 gccagccaca gccggccgac cggcgagcca tccgcctcga ccacgtagag cgtgctgccc
      121 gggcgtccca gggcggaggc aacgtcggca tcggccttgg ctcgcgccgc ctcgggagtc
      181 tggcctccct gctgctggac ggcctcggca tagcccgccg ttgacgccgc gatccacgcc
      241 tcacgctcgt cgggccgcac cggccgaatg gtgaccgtag gttccacgtc tctgatcgta
      301 cgcgcgtgac accgggggtg gtgcgctcac cggatggctc taacggccaa gaagacaggc
      361 acatcacccc ctagttggcc gccaggaccg gccggtgacc gccgccgccg agagtggcgc
      421 gtttacgggg gctgcctggc tgttagagcc acgccgtggc cacgcgacca gtgggcgccc
      481 ggccggtggc tgggcgagcg acggtccccc gggtgtgcaa ccccggtcac gggacgcgtc
      541 ccacgcggct ggtaccctag ccgcccatga gcatcgatcg ggtctacgtt gtgggcgccg
      601 gcctgatggg ccacggcatc gcccaggtca gcgcggccgc cggcaagcag gtcaccctgg
      661 ccgaccgcac cgccgagctg gccgacaagg gccgtcagcg catcgccggc aacctgggac
      721 gccaggtcga gaagggcaag ctggagcagt cggacgcgga ggccacgctg gcccgcatct
      781 ccaccgcgga gggcacggac gaggtcgccg gccacggcct ggtggtcgag gccgtcttcg
      841 aggacgagcc gctaaagcgg gagacgtggg cggccatcag cgagcgcgcc gatccggaca
      901 ccatcttcgc ctccaacacg agcagcatca gcatcaccgc cctggccacc gcgacggacc
      961 ggccagagag gttcatcggt ctgcacttct tctcacccgt cccggtcatg aagctcatcg
     1021 agatcatccg cggcctggag accgacgaca ccacccacgc ggccgcccgc accttcgccg
     1081 aggacgtggg caagaccgtg atcgagtcca aggacatgcc cggcttcctg gtgaaccgca
     1141 tgctggcgcc gctgatcaac gaggcgatct tcgcctacgc ggaggggacg ggcagcgccg
     1201 aggacatcga caccggcgcc aagctgggcc tgaaccaccc catgggcccg ctggagctgt
     1261 ccgacttcat cggcaatgac gtgatgctgg gggtgatgga ggtcctgtac cgcggcttcg
     1321 gcgacccgaa gtttcgcgcc gcgcccctgc tgcgccagat ggtcaccgcc ggccacctgg
     1381 gccgcaagag cggccgcggc ttctaccgct acgacgagaa gggcaacagg atcgccggat
     1441 gacgaccacc accgcgccgc gccaggaccc cttcgccctg accgaggagg aacgcagcgt
     1501 gcgcgacacg gcgcgcgcct tcgccgagcg cgagctggcg ccgacggttg cccagcgcga
     1561 cgaggaggag cgctacgacc gcgccctgtt cgaccgcatg ggcgagctgg gcctgaccgc
     1621 catcccctac ccggaggagt acggcggtgc cggcatgggc agcttcgcct gggtacttgg
     1681 ctgcgaggag atcgcggcgg cggatatggg gatggcggtg tcgctatcgg tccatgtgct
     1741 tgcgcagctg tgcattcatt ccttcgggac cgatgagcag aagcagcgcc tgctcccagc
     1801 catgaccgcc gggcgtcacc tgggcgcctt cgcgctgacc gagccgggag ccggctcgga
     1861 cgcatcggcc atcagcctac gcgcggaccg gaccgatgat ggctacgcgc tcaccggcac
     1921 caaggtctgg atcaccaacg gtgccgatgc cgaggtcgcg atcgtattcg ccaccgtcga
     1981 ccgctcgcgc ggccgcgacg gggtcaccgc cttcgtggtc gagcgtggga cgccaggctt
     2041 ccgcgtgggt gggcacgagc ggaagatggg gatccgggac tcggcgtcgg tggagctggt
     2101 cttcgaggag gcagccgtgc cg
//
LOCUS       DATACA010000076         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1698103, whole genome shotgun sequence.
ACCESSION   DATACA010000076 DATACA010000000
VERSION     DATACA010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698103"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VHK28_01710"
     CDS             <1..800
                     /locus_tag="VHK28_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2140977.1"
                     /translation="MRTNRSLLAAGWLAALIALTAGLTQGSDEVRAPLHDPATPVGQA
                     QEPETDGRQLYLQACASCHGPEGAGSPLGPSLLGVGAASVDFQLTTGRMPFAGPPGSQ
                     TRRKPPAFSPQQIDALVDYVVSLGNGDGPDIPEVALREELINRGLDLYIGNCAPCHGS
                     TGNGGAVGGGALAPPLDRATPVQVVEAMLSGPGQMPVFRYSQEDQDAVATYVEYLRRA
                     PNPGGFSIGGIGPVPEGFVSWVLGMASLLAVAYLVGRRWDRSGKREP"
     gene            797..1648
                     /locus_tag="VHK28_01715"
     CDS             797..1648
                     /locus_tag="VHK28_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2140978.1"
                     /translation="MTSERGIQALLVASMLASAGLVAVYFTGWGGAMAEGLLLTVALG
                     GVGAAIVVWSVTLMHAPAEIEERHPLVSAEEQRTAVQEALDLESITRRRFLVRMLVGA
                     GAFLGAALVLPVFSLGPRPERELFETGWRAGTRLVGADGEPIRVADLTEQAVATVFPE
                     GAVGRPDSGTMLIRLRPSDLRLPAGREAWAPEGYVAYSKLCTHAGCPVGLYRAEDQVL
                     ICPCHQSTFDVTRGAVPVFGPAARPLPQLPLEVDADGFLVASDTFDAPVGPSFWNLTH
                     DQDEAAG"
     gene            1650..2999
                     /locus_tag="VHK28_01720"
     CDS             1650..2999
                     /locus_tag="VHK28_01720"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX2140979.1"
                     /translation="MLHWVDSRLGVASWTRSGLRKIFPDHWSFLLGEVALFCLVVLVL
                     TGVFLTLFYVPDATLVEYEGSYGPLVGHEVSRAYESVLALSFDVRAGLVMRQIHHWAA
                     LVFVAAITVHLMRVFFTGAFRRPREINWLVGIGLLLLTLAMGITGYSLPDDLLSGTGL
                     RIIYSAIIGVPLIGPYAAFLLFGGEFPTETIISRLFVFHVMLLPFLLIGGVLVHLAIL
                     WRQKHTQWAVPGATEHNVVGRPFWPSQVFKSTGLLLLTAAVLAFMGGVVQINPIWTYG
                     PFDPNTVSAPAQPDWYVGWLDGALRLFPPIEFTILGITVPTVFVPGIVIPGIAFGIMT
                     LWPFIEPRLTGDHGEHHILDRPRDNALRTSIGVAGLLVFVVLTVAAGNDVIALIFNAP
                     VEVMTELLRIAFFVVPVLGFLVTYRICVELQRKTDVSDAHQPVRLRRTASGGFEEIE"
     gene            complement(3013..>3630)
                     /locus_tag="VHK28_01725"
     CDS             complement(3013..>3630)
                     /locus_tag="VHK28_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140980.1"
                     /translation="RDDPIRPGDWSPKDHQAHLTAWKARQARRFDSAREGIEVPAITE
                     GDETDAINAELHATRADWTWEAIAREADEVSERLVQAIEATDPEMIAATDRLLGGTFG
                     NGAFHAIAHFGWLIEADVGIDTERVDAFLDETLALVRRSAVPRNEVAAAVYNTACHYA
                     LRGQLNEARPLLREAFSLSPELLEWSRQDEDLRDIRDELAALAPT"
BASE COUNT          451 a         1281 c         1258 g          640 t
ORIGIN      
        1 agatgcgcac caaccggtcg ctcctggcgg ccggctggct ggcggcgctg atcgcgctga
       61 cggccggcct gacccaggga agcgacgagg tccgagcgcc tctccatgat cccgcgaccc
      121 cagtcggcca ggcgcaggag cctgagaccg atggccggca gctgtacctc caggcctgcg
      181 cctcctgcca tggtcccgag ggagctggct cgccgctcgg ccccagcctt ctgggcgtgg
      241 gcgccgcgtc cgtcgacttc cagctgacga cgggccggat gccgttcgcg gggccgcccg
      301 gcagccagac ccggcgcaag ccacccgcct tctcgcccca gcagatcgat gcgctggtcg
      361 actacgtcgt ctcgctgggc aacggtgacg ggccggacat cccggaggtc gctctgcgcg
      421 aggagctcat caaccgcgga ctggacctct acatcggcaa ctgcgccccg tgccacggct
      481 cgacgggcaa cggcggggcg gtcggcggcg gagcgctggc gcctccgctc gatcgggcca
      541 cgcccgtgca ggtggtggag gccatgctct ccggtcccgg ccagatgccc gttttccgct
      601 acagccagga agaccaggac gcggtggcca cctacgtcga atacctgcgg cgcgcgccga
      661 atcccggcgg cttctccatc ggcggcatcg gtccggtccc ggaggggttc gtgagctggg
      721 tgctcgggat ggcgtccctg ttggcggtgg cctacctggt cggcaggcgc tgggaccgat
      781 ccgggaagcg ggagccatga ccagcgagcg gggaatccag gcgctgctgg tggcctccat
      841 gctggcctcg gccgggctgg tggccgtcta cttcaccggt tggggcggag ccatggccga
      901 gggcctgctg ctgacggtgg cgctgggcgg ggtcggcgca gcgatcgtgg tctggtccgt
      961 gacgctcatg catgcgccgg ccgagatcga ggagcggcat ccattggtct ccgcggagga
     1021 gcagcgcacc gcggtgcagg aggcgctcga cctggaatcc atcacccgac gtcgcttcct
     1081 ggtgcgcatg ctggtcggtg ccggcgcctt cctgggcgcc gcactggtcc tgccggtgtt
     1141 ctcgctcggc ccgcgcccgg agcgcgagct gttcgagacc ggctggcggg cggggactcg
     1201 gctggtcggc gcggacggtg agccgatccg ggtggccgac ctgaccgagc aggcggtcgc
     1261 caccgtcttc ccggagggtg cggtcgggcg gccggacagc ggaaccatgc tcatccgcct
     1321 gcgcccgtcc gacctgcggc ttccggccgg gcgcgaggcc tgggcgcccg agggctatgt
     1381 cgcctactcc aagctgtgca cgcacgcggg ctgcccggtt ggcctgtacc gggcggagga
     1441 ccaggtcctc atctgcccct gccaccagtc gacgttcgac gtcacccgcg gcgccgtccc
     1501 cgtcttcggt ccggccgcgc ggccgctgcc gcagcttccg ctggaggtgg acgcggacgg
     1561 tttcctggtg gcgtcggaca cctttgatgc accggtgggc ccatccttct ggaacctgac
     1621 ccacgaccag gacgaggcgg ctggctgaca tgctgcactg ggtcgactcc cgtctcggcg
     1681 tcgcgtcatg gacgcgctcc ggcctgcgca agatctttcc ggaccactgg tcgttcctgc
     1741 tcggcgaggt cgccctcttc tgcctggtgg tgctggtgct gaccggcgtc ttcctgaccc
     1801 tcttctacgt cccggacgcc accctggtcg agtacgaggg ctcgtacggc ccgctggtcg
     1861 ggcacgaagt ctcgcgagcg tacgaatcgg tcctggccct gtcgttcgac gtgcgcgccg
     1921 ggctggtcat gcgccagatc catcactggg cggcgctggt cttcgtggct gcaatcaccg
     1981 tgcacctgat gcgcgtcttc ttcaccgggg cattccgccg gccccgggag atcaactggc
     2041 tggtgggcat cggcctgctg ctgctgacct tggcgatggg catcaccggc tattcgctgc
     2101 cggacgacct gctgtcggga accggactgc ggatcatcta ctcggccatc atcggcgtcc
     2161 cgctcatcgg tccatacgcc gccttcctcc tgttcggcgg cgagttcccg acagagacga
     2221 tcatcagccg cctcttcgtg ttccacgtca tgctgctgcc cttcctgctg atcggtgggg
     2281 tgctcgtcca cctcgccatc ctgtggcggc agaagcacac ccagtgggcc gtccccgggg
     2341 ccaccgagca caacgtggtc ggccgcccgt tctggccgtc gcaggtcttc aagtcgaccg
     2401 ggctgctgct gctcaccgcc gccgtgctgg ccttcatggg cggggtggtg cagatcaacc
     2461 ccatctggac ctacggcccg ttcgacccca acacggtgag cgcccccgcc cagccggact
     2521 ggtacgtcgg ctggctggac ggcgccctgc ggctgttccc gcccatcgag ttcacgatcc
     2581 tgggcatcac cgtgcccacc gtgttcgtgc cggggatcgt gatccccggc atcgccttcg
     2641 ggatcatgac tttgtggccg ttcatcgagc cgcgtctgac cggtgaccat ggcgagcacc
     2701 acatcctgga tcggccgcgc gataacgccc tgcgcacgtc cattggcgtg gccggcctgc
     2761 tggtcttcgt ggtgctcacc gtggcggccg gcaacgatgt gatcgccctc atcttcaacg
     2821 ccccggtgga ggtgatgacc gagcttctgc ggatcgcctt cttcgtggtt ccggtgctgg
     2881 ggttcctggt cacctaccga atctgcgtgg agctccagcg caagaccgat gtctccgacg
     2941 cgcaccaacc ggtgcgcctg cggcgcacgg cgagcggcgg attcgaggaa atcgagtagg
     3001 gctttttacg gcctacgttg gcgccagcgc cgccagctcg tcacgtatgt cccgcaggtc
     3061 ttcgtcctgc cgtgaccatt cgagcagctc gggtgacagg ctgaaggcct cgcgcaggag
     3121 cgggcgcgcc tcgttcagct gtccccgcag cgcgtaatgg caggccgtgt tgtagaccgc
     3181 ggccgccacc tcgttccgcg gcacagcgga gcggcggacg agtgccaggg tctcatccag
     3241 gaaggcatcc acgcgctcgg tgtcgatgcc cacgtccgcc tcgatcagcc agccgaagtg
     3301 ggcgatggcg tggaaggcgc cgttcccgaa cgtgccgccc agcagacgat ccgtggcggc
     3361 gatcatctcg gggtccgtgg cctcgattgc ctgcaccagc cgctcgctga cctcgtccgc
     3421 ctcgcgggcg atggcctccc acgtccagtc ggcgcgcgtc gcgtgcagct cggcattgat
     3481 ggcgtccgtc tcgtcgccct cggtgatggc cggcacctcg atcccttccc gggcggagtc
     3541 gaagcggcgt gcctggcgcg ccttccaggc ggtgaggtgg gcctgatggt ccttcggcga
     3601 ccagtcgccg ggacggatcg ggtcgtcgcg
//
LOCUS       DATACA010000077         3156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2340614, whole genome shotgun sequence.
ACCESSION   DATACA010000077 DATACA010000000
VERSION     DATACA010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2340614"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VHK28_01730"
     CDS             <1..657
                     /locus_tag="VHK28_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2140981.1"
                     /translation="ELLDEFRDIPLVAISDSQGRWWPKNNWQATVHHGLPLENAPFSA
                     EPGGYLAFVGRVAPEKGIEHVVTLARETGVPLRMAAKVFEVEEQELFDRVVAPAVEEG
                     VVSFEGELPTDQRDDLMRGALAVVMMGAWPEPFGLVAIESMATGTPVIARRAGALPEI
                     VEHGQTGFLVDDMTEACLAVRSVAELDRTAIRHRSLERFSVQRMVDDYEEVYHRVAGA
                     "
     gene            742..921
                     /locus_tag="VHK28_01735"
     CDS             742..921
                     /locus_tag="VHK28_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140982.1"
                     /translation="MLEQIVSVAVVAILCATAFLYAALISRRVRERRVRNRIYETIHL
                     TAVVRTTIAQRRSLA"
     gene            1100..2065
                     /locus_tag="VHK28_01740"
     CDS             1100..2065
                     /locus_tag="VHK28_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140983.1"
                     /translation="MVASAPPQLDEATVAWLFDTQEPAVRRLARRELLHASSPDDDQA
                     VLDGPIVAALLDGQQPDGGFGVGPYTKWTGAHWRLVSLVELGVPAGEPRAVAAAESVL
                     AWLTGAAHRRNVPVVNGLARRCGSQEGNALAVASRLGLAGDERTRLLASSLIEWQWPD
                     GGWNCDRRPEAHRSSFHESLVPTWGLHEYACATNDTDAAAAARRGAELFLEHRLFRRL
                     ADGEPIHREWLTMHWPPYWHYDVLQAMVVLARMGLAADPRTADALELISSRRRQDGRW
                     QASTRWWRPPPSTRMPEVLDWRWDDTADRMVTLRALSVLRAGAAG"
     gene            complement(2157..2717)
                     /locus_tag="VHK28_01745"
     CDS             complement(2157..2717)
                     /locus_tag="VHK28_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX2140984.1"
                     /translation="MSNERVPQHEQSLIGHQRTTPWEDARERLANPGSEEYSHAWLAT
                     VRPNGRPHLMPLISFWMEGGLHFIVGEGTRKGRNLASNPWCVIGTENRKVPSLDVIVE
                     GRAEPLTDPDEVRRIAEVFASRNWPLEVRGVEVHGPNAPTAGPPPYRIYRLTPSRAFG
                     LPGNYGLFQVEPGDLPKPTRWDFAAD"
     gene            2816..>3156
                     /locus_tag="VHK28_01750"
     CDS             2816..>3156
                     /locus_tag="VHK28_01750"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2140985.1"
                     /translation="MRGRTKGHTSADRWARHDWWGATIDTPDPLALARFYATLLDWEV
                     TGDDGDRSSAAINPGEGVAYVGFEQVDDYEPPVWPGQRGKQRMMMHLSFEVGDLDAAV
                     QHAVELGARLA"
BASE COUNT          474 a         1065 c         1116 g          501 t
ORIGIN      
        1 gagctcctcg acgagttccg cgatatcccg ctggtggcca tcagcgacag tcagggccga
       61 tggtggccga agaacaactg gcaggcgacc gtgcaccacg gcctgccgct ggagaacgcc
      121 ccattctccg cggagcccgg cggctacctg gccttcgtcg gccgcgtagc tccggagaag
      181 ggcatcgagc acgtggtcac gctggcgcgt gagaccggcg tcccgctgcg catggccgcc
      241 aaggtcttcg aggtcgagga gcaggagctc ttcgaccggg tggtggcacc ggccgttgag
      301 gagggcgtgg tcagcttcga gggcgagctg ccgaccgacc agcgcgacga cctcatgcgc
      361 ggcgccctgg cggtggtcat gatgggcgcc tggccggagc ccttcggcct ggtggcgatc
      421 gagtcgatgg cgaccgggac gcccgtcatc gcccgccgcg cgggagcact cccggagatc
      481 gtggagcacg ggcagacggg cttcctggtg gacgacatga ccgaggcctg cctggccgtc
      541 cgatcggtgg cggagctgga ccgcacggcg atccggcatc ggtccctgga gcgtttcagc
      601 gtgcagcgca tggtggatga ctacgaggag gtctaccacc gggtggccgg cgcctgatgg
      661 ccgcgccagc cgccgtgcgt acccggcggc gggtaccaat gggccatggc cgccgtcaat
      721 ggggcagggt gcaatgcgcg catgctcgag cagatcgtct cggtcgccgt ggtcgcgatc
      781 ctgtgcgcca ccgccttcct gtacgccgcg ctcatctcgc gtcgggtgcg cgagcggcgc
      841 gtacgtaacc gcatctacga gacgatccac ctgaccgccg tcgtgcgcac cacgatcgcc
      901 cagcgccgca gcctggcctg agcggctcgt tgcaccctta acttctggca cgcggtgtgc
      961 gtgccctgct ccccatcgcg ggcagcggac cccgtaacga accgatgctc gcgcagcccg
     1021 gcccggaccg aggggtgaag gttcgggtct ccatctgtcc agcggaatca ttgcgaggct
     1081 gtggcatgct ggccccagca tggtcgccag tgcgccacca cagctcgacg aggccaccgt
     1141 cgcgtggctg ttcgacacgc aggagcccgc tgtccgtcgg ctcgcgcgac gcgagctcct
     1201 ccacgcgtcc agccctgatg acgaccaggc agtcctggac ggccccatcg tcgccgccct
     1261 gctcgacggt cagcagccgg acggtgggtt cggtgtgggt ccctacacca agtggaccgg
     1321 cgctcactgg cggctggtgt cgctggtgga gctcggcgtg ccggccggtg aaccgcgcgc
     1381 cgtggccgcc gccgaaagcg tgctggcctg gctgacgggt gctgcccacc gacgcaacgt
     1441 cccggtcgtc aatggcctgg cccgccgctg cgggtcgcag gagggcaacg ccctggcggt
     1501 cgcgtcccgg ctcgggttgg ccggcgacga gcgcactcgc ctgctggcga gctcgctgat
     1561 cgagtggcag tggccggacg gtggctggaa ctgcgaccgg cggccggagg cccatcggtc
     1621 cagcttccac gagagcctgg ttcccacctg gggcctgcac gagtatgcgt gcgcgacgaa
     1681 tgacaccgat gccgccgcgg cagcccggcg cggcgccgag ctcttcctgg agcaccgcct
     1741 cttccgccgc ctggccgatg gcgagccgat ccaccgcgag tggctgacca tgcactggcc
     1801 gccgtactgg cactacgacg tcctgcaggc catggtcgtc ctggcgcgca tggggctggc
     1861 ggcagatccc aggaccgccg acgctctgga gctgatctcc agccggcgcc ggcaggacgg
     1921 acgctggcag gccagcaccc gttggtggag gccgccgccc agcacgcgca tgccggaggt
     1981 cctcgactgg cgatgggatg acactgcgga ccggatggtc acgcttcgcg ccctcagcgt
     2041 gctccgagcc ggtgcggccg gctgagcgct ggacgaggac cgcggcagcc gggcgctcaa
     2101 gggagtgccc ggccgaaggc ggctcttcgt cgttacaaag ggctagcgac cgtccttcag
     2161 tctgcagcga agtcccatcg ggttggcttg ggcaagtcac cgggttcgac ctggaacaac
     2221 ccatagttgc ctgggaggcc aaacgcccgg gacggagtca gcctgtagat tcgatatggc
     2281 ggaggaccgg cggtcggtgc gttcggccca tggacttcga cgccgcgaac ctcgagcggc
     2341 cagttgcgtg atgcgaatac ttcagctatc cggcggacct cgtccgggtc ggtgagcggc
     2401 tcggccctgc cttcgacgat tacgtcgagc gacggcacct tccggttctc ggtcccgatg
     2461 acgcaccacg ggttcgaggc gagattgcgg cctttgcggg ttccctcccc gacgatgaag
     2521 tggagcccac cttccatcca gaacgagatc aggggcatca gatgcggccg accgttcggt
     2581 cgaacggtcg ccagccatgc gtggctgtat tcctctgatc ccgggttcgc cagccgttcg
     2641 cgagcatctt cccagggcgt cgttcgctgg tgaccaatca gtgactgctc gtgctgcggc
     2701 acacgttcgt tcgacatgtg agcctccttt ggagaatctc ggcaatcgat caacgtaggt
     2761 atgacccggg ctgccgcccg gaaatccggt gacaggactg ccgtagcatg ccgacatgcg
     2821 cggacgaacg aaaggacaca cctcagccga tcggtgggcg cgccatgact ggtggggggc
     2881 aaccatcgac acccccgacc ccttggcgct cgctcgcttt tacgcgacgc tgctcgactg
     2941 ggaggtgacc ggggacgacg gcgaccggag cagtgccgca atcaacccgg gtgaaggcgt
     3001 ggcctatgtc ggcttcgagc aggtcgatga ctacgagcct ccggtatggc ccgggcaacg
     3061 tgggaagcag cggatgatga tgcacctgag cttcgaggtc ggcgacctcg acgcggcggt
     3121 tcagcacgcg gtcgagctcg gcgcgcggct cgccga
//
LOCUS       DATACA010000078         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1698355, whole genome shotgun sequence.
ACCESSION   DATACA010000078 DATACA010000000
VERSION     DATACA010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698355"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1564
                     /locus_tag="VHK28_01755"
     CDS             26..1564
                     /locus_tag="VHK28_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2140986.1"
                     /translation="MTEQRPSPGRLRAALSGLISGAVGLGLAELFAGLVPGAPSPVTA
                     VGALVISLQPPNAKQLVVDLFGEADKLVLNLMVLAVALALGAGLGILARRHPGPATAG
                     FVAVGGVVLFAALRDPLNDGLLAVVAVVLAVGASLLVLRRLMASGTGEPRPAEMPDYA
                     RRRFLGQAVALGAVAAGSGVLGRVLLENRAAGVASMPAVPQPGSSVPPPPAGASLNVP
                     GITPLVTPNDSFYRIDTALLTPRVDVAEWRLTVDGMVERPFTIDYDELVAMPLVEQYV
                     TIACVSNEVGGRLVGNALWRGVRLRDLLERAGVMSGATQVVGRSLDGWTAGFPTAWLE
                     AEGRESLVAVAMNGEPLPPQHGYPARLIVPGLFGYVSATKWLSNIHLTTWESFDGYWV
                     PLGWAKEGPILTQSRIDVPRDRANLEAGEVVVAGVAWAPDRGISAVEVRVDEEEWQPA
                     ELAAPISDATWVQWRYRWVAAPGDHVLRVRATDGTGQVQAEERTRPAPDGARGHHTID
                     VQVG"
BASE COUNT          221 a          599 c          635 g          265 t
ORIGIN      
        1 aattcgacgc ttgactgata gcgtcatgac ggagcaacga ccctctcccg gccgattgcg
       61 ggccgcgctg tccgggctga tttccggtgc cgtggggctg gggctggccg agctgttcgc
      121 ggggctggtg cccggagcgc cttcgccggt caccgcggtc ggtgcactgg tcatctccct
      181 ccagccgccg aacgccaagc agctggtggt cgacctgttc ggcgaggcgg acaagcttgt
      241 cctcaacctc atggtgctgg cggtggcatt ggcgctgggt gcgggactcg gcatcctggc
      301 ccgccgccac ccgggtccgg cgacggccgg attcgtggcg gtcggcggcg tcgtgctctt
      361 cgcggcgctg cgcgatccgc tcaacgacgg cctgctcgcg gtggtggccg tcgtcctggc
      421 cgtgggcgcg tccttgctcg tcctgcggcg actcatggcg agcggcacag gcgagccacg
      481 gccggccgag atgccagact acgcccgccg ccggttcctg ggccaggcgg tagctctcgg
      541 cgctgtggcc gccgggtccg gggtgctggg acgcgtgctg ctcgagaacc gtgctgccgg
      601 cgtcgccagt atgcctgccg taccgcagcc cgggtcctca gtgccacctc cacccgccgg
      661 cgccagcctg aacgtgcccg gcatcacgcc cctggtcacg ccgaacgaca gcttctatcg
      721 catcgacacc gcgctgctca cgccgcgggt ggacgtggcc gagtggcgcc tcaccgtgga
      781 tggcatggtg gagcgaccct tcaccatcga ctacgacgag ctggtagcca tgccgctggt
      841 cgagcagtac gtgaccatcg cctgtgtcag caacgaggtc ggtgggcggc tggtgggcaa
      901 cgcgctgtgg cggggagtcc ggctgcgcga cctcctcgag cgcgccgggg tgatgtccgg
      961 cgccacgcag gtcgtcggcc gctccctgga cggctggacg gccggctttc cgaccgcctg
     1021 gttggaggcg gagggacggg aatcactggt ggcggtcgcc atgaacgggg agccgctgcc
     1081 gccgcagcac ggttatccgg cccgcctgat cgttccggga ctgttcgggt acgtatcggc
     1141 aaccaagtgg ttgtcgaaca tccacctcac cacctgggag agcttcgacg gctactgggt
     1201 gccgctgggc tgggccaagg agggcccgat cctgacccag tcgcgcatcg acgtgccccg
     1261 tgaccgcgcc aatctggagg ctggcgaggt ggtcgtggcc ggggtggcat gggcgccgga
     1321 ccgcggcatc tcggccgtcg aggtgcgggt cgacgaggag gaatggcagc cggccgagct
     1381 ggcagccccg atctccgatg ccacctgggt ccagtggcgg taccgctggg tggcggcccc
     1441 gggcgaccac gtgctgcgcg tgcgtgccac cgacgggacc ggccaggtgc aggccgagga
     1501 gcgcacgcgc ccggccccgg atggcgcccg cggccaccac accatcgacg tccaggtcgg
     1561 ctgacgctcc gcggcgttga cgcgaccggc agaatcgggg acgtgccaac cgacggatcg
     1621 ccgctcgtca tcgtcaaccc gatcgccggc aacggccgcg cccaccacct cgcccccaag
     1681 ttggcggact ggctggtacg cgagcagcgt ggcgcgcggc
//
LOCUS       DATACA010000079         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2823210, whole genome shotgun sequence.
ACCESSION   DATACA010000079 DATACA010000000
VERSION     DATACA010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2823210"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..637
                     /locus_tag="VHK28_01760"
     CDS             128..637
                     /locus_tag="VHK28_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX2140987.1"
                     /translation="MLWLAIVFGGLLTVARLHQLEADDGALDELARYPVERGTIYVGK
                     AMAAGLAMIALGAVLLPVVAILYGLDLASALPALLATLLLGTIGFAAVGTFYAGVTVR
                     LRAREVMLPLLMLPVIAPLLLAAVSATAAALAGDPFGELAAWLQLLIGFDVVMLIAGA
                     AGYGYLLEE"
     gene            637..1377
                     /gene="ccsA"
                     /locus_tag="VHK28_01765"
     CDS             637..1377
                     /gene="ccsA"
                     /locus_tag="VHK28_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX2140988.1"
                     /translation="MATRVLGGVALLAVLAASVNALFIVPADLNQGDAQRIMYVHVPS
                     AWLAYLSFGVTALAGVGYLWKRDLRLDAVAVAGAEIGVMFTAFAIWGGMMWGRPVWGV
                     FWQWEDPRLTTTALLLALYVGYLLFRRLTDDPHRRAARAAVVGLVAAVDIPIVHFSVE
                     WWRGLHQGPTIGSPERALDPAAPMQFVITLLAMLGAFTLAWAWLMVRRYQLARVEWQI
                     EEHGRLGRISAARRAAAVAAPPAEETAG"
     gene            1374..1568
                     /locus_tag="VHK28_01770"
     CDS             1374..1568
                     /locus_tag="VHK28_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140989.1"
                     /translation="MIESWITLPQDIWFIVAGWGVILGGLGLYAATLLRRLAAARDAS
                     LRIRREAESAESADVRDDRR"
     gene            1565..1984
                     /locus_tag="VHK28_01775"
     CDS             1565..1984
                     /locus_tag="VHK28_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX2140990.1"
                     /translation="MRRWPLLLGVAAVGGVIAWLAFSGIGSALVYYQTPSELLARGDG
                     AVGVPVRLGGLVKEGSISGPATDLTFVLTDGTSEVTVHSTVAPTSSFRDGTGAVVQGS
                     LGPDGVFEADQVIVKHDENYVAPSEGELPSQVIDPGQ"
BASE COUNT          234 a          718 c          743 g          343 t
ORIGIN      
        1 agcgcgacca tggccagccc accggccatg gccttgccca ggtagatggt cccccgctcc
       61 accgggtagc gcgccagctc gtcaagcgcg ccgtcgtcgg cctccagctg gtgcagccgc
      121 gccgggcctg ctgtggctgg ccatcgtctt cggcgggctg ctgaccgtgg cgcgcctgca
      181 ccagctggag gccgacgacg gcgcgcttga cgagctggcg cgctacccag tggagcgggg
      241 gaccatctac gtgggcaagg ccatggccgc tgggctggcc atgatcgcgc tgggcgccgt
      301 gctgctgccg gtggtcgcca tcctgtacgg cctggacctg gcctcggcgc tgcccgcgct
      361 gctcgcgacg ctcctgctgg ggaccatcgg cttcgccgcc gtgggcacct tctacgccgg
      421 ggtcaccgtg cgcctgcggg cacgggaggt catgctccca ctgctcatgc tgccagtcat
      481 cgccccgctg ctgctggccg cagtgagcgc tacggccgcc gcgcttgccg gcgatccgtt
      541 cggcgagctg gccgcctggc tgcagcttct gatcggcttc gacgtcgtga tgctcatcgc
      601 cggggccgcc ggctacggct acctgctgga ggagtgatgg cgacacgcgt gctgggtgga
      661 gtggcgctgc tggccgtgct ggcggcatcg gtcaatgcgc tgttcatcgt gcctgccgac
      721 ctgaaccagg gcgatgccca gcggatcatg tacgtccacg tcccgtccgc ctggctggcc
      781 tacctgtcgt tcggcgtcac cgccttggcc ggcgtgggct acctgtggaa gcgggacctg
      841 cgcctggatg cggtggcggt ggcgggggcc gagatcgggg tcatgttcac ggccttcgcc
      901 atctggggcg gcatgatgtg gggccgtccg gtgtggggcg tcttctggca gtgggaggac
      961 ccgcgcctca ccacgaccgc gctgctgctg gcgctgtatg tcggctatct gctcttccgg
     1021 cgcctgaccg atgatcccca ccgccgcgcc gcccgcgccg cggtggtggg cctggtggcc
     1081 gcggtcgaca tccccatcgt gcacttcagc gtggagtggt ggcgcggcct gcaccagggg
     1141 ccgaccatcg gctcgccgga gcgtgccctg gacccggccg cccccatgca gttcgtgatc
     1201 accctgctgg ccatgctcgg cgcattcacc ctggcctggg cctggctcat ggtgcgtcgc
     1261 taccagctgg cgcgggtcga gtggcagatc gaggagcacg gacggctggg gcgtatcagt
     1321 gcggcacgcc gcgccgcggc tgttgccgct ccgccggccg aggagacggc cggatgatcg
     1381 agagctggat cacccttcca caggacatct ggttcatcgt cgccggctgg ggcgtcatcc
     1441 tcggcggcct agggctgtac gccgccacgc tgctgcgccg cctggccgct gcgcgcgacg
     1501 cgtcacttcg catccgccgc gaggcggaga gcgcagagag cgcggacgtg cgggacgatc
     1561 gccggtgagg cgctggcccc tgctgctggg cgtggccgcc gtgggcggcg tgatcgcctg
     1621 gctcgccttc agcggcatcg gcagcgccct ggtctactac cagacgccgt ccgagctcct
     1681 cgcccgcggg gatggcgcgg tcggcgtccc ggtgcggctg ggaggtttgg tcaaggaggg
     1741 cagcatctcc ggcccggcca ccgacctcac cttcgtgctg accgacggca ccagcgaggt
     1801 caccgtgcac agcaccgtgg cacccaccag ctcgttccgt gacgggaccg gcgccgtggt
     1861 gcagggttcg ctgggccccg acggcgtgtt cgaggccgac caggtgatcg tcaagcacga
     1921 cgagaactac gtggcgccca gcgagggcga gctgccctcg caggtgatcg acccgggcca
     1981 atgatcccga acctgggcca catcgcggtg gttgtcggtc tggcctccac cctgttcg
//
LOCUS       DATACA010000080         3862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2501655, whole genome shotgun sequence.
ACCESSION   DATACA010000080 DATACA010000000
VERSION     DATACA010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501655"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHK28_01780"
     CDS             <1..190
                     /locus_tag="VHK28_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning ATPase"
                     /protein_id="HEX2140991.1"
                     /translation="LAREMLQNLREIGDHRVFNTMIKNTVKLGEAPLTGKPVTEYATN
                     SAAARAFRELAREVIELG"
     gene            183..1154
                     /locus_tag="VHK28_01785"
     CDS             183..1154
                     /locus_tag="VHK28_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2140992.1"
                     /translation="MASKRPAFTENIHRLFDEAAATDLAPGIVSLIESRATRAQIREL
                     PLDKILPNPAQPRLSYEEDSLVELADSIREHGVLQPILVRPVGSKFELIAGERRWRAS
                     RQAERESIPAIVVEFDDQTALEVSIIENLQREDVSPLEEAAMFRKMTDLGYSVRQLAQ
                     KIGKDKGYVENRIRLNDAPPEIRELVSVRKDTLSHAYELMRIPDERTRRRLAAKVAAG
                     DLTLAKLRQLTGSDADGEGRPKRRGRTVAGEVAVARAAKATDDALVANRGKLAAAIDE
                     LVELLRQPELIEQIPDTNRDNMARSLTVTKLKLENAIAILRATREPA"
     gene            complement(1170..1526)
                     /locus_tag="VHK28_01790"
     CDS             complement(1170..1526)
                     /locus_tag="VHK28_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140993.1"
                     /translation="MIEGQEATYREMVAAQSDEQVDRWAADLFIDFAKRMGVGTAIAS
                     FNRIAGLDSRGFQRAFMVGGGPDHVIGIDTAGSLAAPIFELPRAIQGLRRIDPEARKK
                     LVDFLVANREVMSYTP"
     gene            1601..2170
                     /locus_tag="VHK28_01795"
     CDS             1601..2170
                     /locus_tag="VHK28_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140994.1"
                     /translation="MTARPIDPRTEDTISYELVRTDRSSGTEQQAGRATFSNGRTRVE
                     ADDDLRVAVEELLGRAFVDRVEADERPRGYRRSGRGIVDMLVPGMPEHFIARMRGLWL
                     SYPDGTVVTARPAPADSHRGPAQSTAIEPVESGPPVTDASVRRATLADSDEILNVRPL
                     LRPNPPLVGLRGDEERTGTPRTTDCGWLV"
     gene            2248..2457
                     /locus_tag="VHK28_01800"
     CDS             2248..2457
                     /locus_tag="VHK28_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2140995.1"
                     /translation="MSLKKYALLPLLIFAVGVIYWFASYEAAGTALLIVFAAAMAVMG
                     WILVPTVDNVGPTAPVDPDFEDPGR"
     gene            2752..>3862
                     /locus_tag="VHK28_01805"
     CDS             2752..>3862
                     /locus_tag="VHK28_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEX2140996.1"
                     /translation="MQPAERGPARGAGRLPVIGSLLLLALSLGLPSPALAQADVTITT
                     QFPAVSFQPGVSASFELTVAAQEPVRVDLAVEGLPEGWSASISGGGNEVQSVFVEPDA
                     PATVTLNLSIPDDAAAEPVQVEVVGTAAGETTTLSLDLVPAAGPGGTVALESDYPSLR
                     GSTDQDFQFNLTLRNDTSQQLTFGLQATGPNGWEVNVQPSGQAQAASVTVDARGTQRL
                     ELTATPPVQATAGAYPLVVEAVAGEHRAATELAVEVTGSLELRLTTPDEVLNTTANAG
                     STRDFDILVVNEGSSPLDGVSLSGRWPTEWDVTFEPATIEALPPGESATATARITPSG
                     NAVAGDYVVTLSAQTEGVDESMEVRVTIETPPIWGI"
BASE COUNT          630 a         1381 c         1298 g          553 t
ORIGIN      
        1 ccttgcccgc gagatgctgc agaacctgcg cgagatcggt gaccatcggg tcttcaacac
       61 catgatcaag aacacggtca agctcggcga ggccccgctg accggcaagc cggtcacgga
      121 gtacgcgacc aactcggctg cggcacgagc gttccgcgag ctggcacggg aggtgatcga
      181 gcttggctag caaacgcccg gcattcacgg agaacatcca ccgcctgttc gacgaggcgg
      241 ccgcgaccga cctggcacca gggatcgtgt cactgatcga gtcacgcgcg acgcgcgccc
      301 agatccgcga gctgccgctt gacaagatcc tccccaaccc ggcccagccc cgcctctcct
      361 acgaggagga ctcgctggtg gagctcgccg actcgatccg agagcacggc gtgctgcagc
      421 cgatcttggt gcgaccggtg ggcagcaagt ttgagctgat cgctggtgag cgtcggtggc
      481 gagcgtcgcg ccaggccgaa cgcgagagca tcccggccat cgtcgtcgaa ttcgatgacc
      541 agacggcgct cgaggtctcg atcatcgaaa acctgcagcg cgaggacgtg tcgccactgg
      601 aagaggcggc catgttccgc aagatgacgg acctcggcta ctcggtgcgt cagctggcgc
      661 agaagatcgg caaggacaag ggctacgtcg agaaccgcat ccgtctcaat gacgccccgc
      721 ctgagatccg tgagctggtg tccgtgcgca aagacactct gagccacgcc tacgaactga
      781 tgcggatccc ggacgagcga acgcgtcgcc ggttggcggc caaggtcgcg gctggtgacc
      841 tgacgctggc caagcttcgg cagctgaccg ggagcgacgc ggacggcgag ggccggccga
      901 agcgccgggg acggactgtg gccggagagg tcgcggtggc gcgcgccgcc aaggccaccg
      961 acgatgccct ggtcgccaat cgcggcaagc tggcggcggc catcgacgag ctggtggagc
     1021 tgttgcgtca gccagagctg atcgagcaga tcccggacac caaccgcgac aacatggccc
     1081 gcagcctgac ggtcaccaag ctcaagctgg agaacgccat cgccatcctg cgggcgaccc
     1141 gcgaaccggc gtagcccagg cgacgccgct cagggcgtgt agctcatcac ctcgcggttc
     1201 gcgaccagga agtcgaccag cttcttgcgt gcctccgggt cgatccgccg caggccctgg
     1261 atcgctcgcg gcagctcgaa gatcggtgcc gccaggctgc cggcggtgtc gatgccgatc
     1321 acgtggtccg ggccgccacc caccatgaac gcgcgctgga agccgcgcga gtcgagcccg
     1381 gcgatgcggt tgaacgaggc gatcgcggtc cccaccccca tccgcttggc gaagtcgatg
     1441 aacaggtcgg ccgcccagcg gtccacctgc tcgtcggact gcgcggccac catctcccgg
     1501 taggtcgctt cctggccctc gatcactccg ctcctccaac tccgtggctg gtcccgattc
     1561 tagccatcgg gcggtcaggg ggtcggctat gctgcccgcc atgacagcgc gccccatcga
     1621 tccgcggacc gaggacacca tcagctacga gctggttcga accgaccgat ccagcggaac
     1681 cgagcagcag gccggacggg ccaccttctc caacggacgg accagggtgg aggccgatga
     1741 cgacctccgg gtggcggtcg aggagctgct gggccgcgcc ttcgtagacc gcgtggaggc
     1801 ggacgagcgc ccgcgcggct accggcgcag cggccggggc atcgtcgaca tgctggtccc
     1861 gggcatgccg gagcacttca tcgcccgcat gcgcggcctg tggctgtcct accccgacgg
     1921 gacggtggtg accgccaggc cggctccggc cgacagccac cgcgggccag cgcagagcac
     1981 cgccatcgag ccagtcgagt ccggcccgcc ggtgaccgat gcgtccgtgc gccgcgcaac
     2041 cctggcggac agtgacgaga tcctgaacgt ccgccccttg ctgcggccca atcccccgct
     2101 ggtcggcctg cggggcgacg aggagcggac gggcacgccc cgcaccaccg actgcggctg
     2161 gctggtctga gtcacagatt taggcatatt tctacccaac atcgccccga gccagcgcgg
     2221 gtccgccggt tgggtatcat ccgcgacatg tcactgaaga agtacgcgct cctgccgctg
     2281 ctgatcttcg ccgtcggggt catctactgg ttcgcgagct acgaggcggc cggcaccgcc
     2341 ctgctgatcg tcttcgctgc ggccatggcg gtcatgggct ggatcctggt gccaaccgtc
     2401 gacaacgtgg gacccaccgc cccggtagat ccggacttcg aggaccccgg ccgctgaccc
     2461 tgggaggccc gggcatgcag atgccgggca actgtggctg ccctagcggt cacccgctaa
     2521 cacagctgcc cggctcctca gtaccaacgc gccacccgcc cgggcgttag gcggccggct
     2581 gtcaaacttc ggacacttcg cgcagccact aggcttgaca acggcccgca acggcgatac
     2641 atctttcggc cggtcgccgc ggcccctgcc gcccggccac ctcctttgat cgcccagcgg
     2701 catgaggcgc tgcgcggggc gtgacaggcg agctcgagct acggaggaac aatgcaacct
     2761 gctgagaggg gtccggcccg tggcgccggc cggctcccgg tcatcggatc cctgctgctt
     2821 ctggcccttt cgctgggcct gccgagtccg gcgctggcgc aggctgacgt gaccatcacc
     2881 acccagtttc cggccgtctc cttccagccc ggggtgagcg cgagcttcga gctcaccgtt
     2941 gccgcccagg aacccgtgcg cgtggacctt gccgtggagg gcctgcccga gggatggagc
     3001 gcgtccatca gcggtggcgg caacgaggtg cagagcgtct tcgtcgagcc ggacgcaccg
     3061 gcgaccgtca ccctcaacct gtcgattccc gacgacgccg ccgctgagcc ggtgcaggtc
     3121 gaggtggtcg gcacggccgc cggcgagacc acgacgctgt ccctcgacct ggttcccgcg
     3181 gccggaccgg gcggcacggt cgccctcgaa tccgattacc cgtccctccg cgggtcgacc
     3241 gaccaggact tccagttcaa cctcacgctc cgcaacgaca cctcgcagca gctgaccttt
     3301 ggcctgcagg ccaccgggcc aaacggctgg gaggtcaacg tccagcccag tggccaggca
     3361 caggctgcca gcgtgaccgt cgacgcgcgc gggacccagc ggctggagct gaccgccaca
     3421 cccccggtcc aggccaccgc cggtgcctac ccgctcgtgg tggaggcggt ggccggtgag
     3481 caccgggctg ccaccgagct ggcggtcgaa gtcaccggct cgctcgagct gcgactgacc
     3541 accccggacg aggtgctcaa caccaccgcc aacgctggct caacacggga cttcgacatc
     3601 ctggtggtca acgagggcag ctcgccgctg gacggcgtgt cgctctccgg ccgttggccg
     3661 accgagtggg acgtgacctt cgagccggcg accatcgagg cgctgccgcc tggggagtcg
     3721 gccaccgcca cggcgcgcat caccccgtcg ggcaacgcgg tcgccggcga ctacgtcgtc
     3781 accctgtcgg cccagactga gggcgtggac gagtccatgg aggtgcgggt cacgatcgag
     3841 acaccgccga tctggggcat cg
//
LOCUS       DATACA010000081         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2742543, whole genome shotgun sequence.
ACCESSION   DATACA010000081 DATACA010000000
VERSION     DATACA010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742543"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(414..1304)
                     /gene="ispH"
                     /locus_tag="VHK28_01810"
     CDS             complement(414..1304)
                     /gene="ispH"
                     /locus_tag="VHK28_01810"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX2140997.1"
                     /translation="MAVHSPTRPEVRIAERAGFCYGVRLAIDKAKKARDEGKPVTTLG
                     QLVHNPGIKADLAERGIATADLADQVEGGTLVIRAHGVPATEMEQLRAKAEGGGELEV
                     IDATCTWVLQSQKAAQELAEAGYTVCIIGHEDHPEVKGVRSYAGQKHVVVDDMDRSTW
                     ERVPRTKKLGVLSQSTILPHKLEEFAAFCLRRCHDLRVVNTVCPVTLTRQDESVKLAA
                     EVDAMVVVGGKNSSNTKELAVKCREICPETIHVADADELAADWGEWLRDKPRIGITGG
                     TSTPEGDLEEVRDRVYALTA"
     gene            complement(1333..2451)
                     /locus_tag="VHK28_01815"
     CDS             complement(1333..2451)
                     /locus_tag="VHK28_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2140998.1"
                     /translation="MPPKQSSFPDPDEGSVRRAALTAATWGIVLLAIVLAAYLIWQLR
                     VVLSLVFLALLLAAAIRGPVERLANLGLPRVIGVVVVYLVVLAVAALGVWLIVPPLVE
                     QASALFENLPQTIEALLGWLRTTLGPLVPGSAIQDAMEGIRSSLDQVLPGIQSALRVP
                     LVVAGVLVNVVLIIFLSIFLILDGGSMWNALLGYFPPGQRERMRGIGETVMSKLGSYV
                     VGQLVIMSATGVGAVVGMLIIGVPYALPLGFLAFLTEAIPLVGPWIGGAPIIAVAFVQ
                     SPVQGLLMAGWILLLQQVEGYVLVPFVQKKAIQVSPTIVLLAVVAGGTIAGVLGALIA
                     IPVVAVTQVLMREVVLPARRRTWQDGEAGAVEPEGASD"
     gene            complement(2481..3137)
                     /locus_tag="VHK28_01820"
     CDS             complement(2481..3137)
                     /locus_tag="VHK28_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2140999.1"
                     /translation="MRTWFFHPGAIITGWLLWLLGPGRVEGLENVPRSGAFVVVANHC
                     SNLDPPFLGSVVGHRTGRVVHFMAKDELRRWPVIGWLAGQAGSFFVRRGSGDRAAQRM
                     ALELLAAGEPLAVFPEGTRSRDGRLKEGRLGAALLATRTGVPVLPVGIAGTHRILAGG
                     SWLPRRGQVTIRIGVPFELPASDQVDRQELRAATDRIMREIAALLPEEQRGRWGTAAD
                     "
     gene            complement(3134..4633)
                     /gene="cmk"
                     /locus_tag="VHK28_01825"
     CDS             complement(3134..4633)
                     /gene="cmk"
                     /locus_tag="VHK28_01825"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX2141000.1"
                     /translation="MPAERLQKVLAQAGVASRRASEDLIAAGRVSVNGRPAELGQSAD
                     PATDEIMVDGRRVGPVERTIHFALHKPAGYVSSARDERGRHSVLRLVEAAPEAAGVRL
                     WPAGRLDVESEGLMVLTNDGAWANRLLHPRYGMEREYAVLVDRAPHPDQLVALLDGVQ
                     LEDGRARLLEARPAAPPREVHRDPAERGTWLRVRLGEGRKREVRRLMAAVGLRVRRLV
                     RVGFGPLALDGLEPGEWRVLDAAEVQALVGSGSPTPGDPGRRRRGRLAVAIDGPSGSG
                     KSTIGFALAERIGATFVDTGLMYRALTLAAVQAGVDPDDEDRLAKLARAVRIEVERPR
                     PDQTDRRETVLLDGKDVTDAARRPRVDRLVSAVSRHAAVRQAMLGIQRAAARRQDTVM
                     VGRDIGTVVLPDATLKVYLTATAEVRAARRAAEMGRPERIETYLAEIRQRDEADSGRP
                     VAPLRKAEGAMVLDTGELGVDACLQAIVEHLPGRPARAAGASVQAKQDR"
     gene            complement(4623..>5094)
                     /gene="scpB"
                     /locus_tag="VHK28_01830"
     CDS             complement(4623..>5094)
                     /gene="scpB"
                     /locus_tag="VHK28_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX2141001.1"
                     /translation="ELASVPHLQAEAELVELADRLEADGRGLRVQRMDDAWQLVTAPE
                     VGARLAAYAAREEGRLSPAALEALSVIAYRQPCTRADVERVRGVDSDYVIRSLLHRRL
                     IVEVGRRDTPGRPVLFGTTFAFLERFGLTSLDDLPPLSSAAAALAAETSVPDAG"
BASE COUNT          778 a         1898 c         1739 g          679 t
ORIGIN      
        1 cgtcgaggta attgaggagc gtcatccgcg gctctccgct gcgacggccc acggtgtgga
       61 tggcgagcgt gccgtcgccg gccgccctgc ccccggagac tcgataggca agccgatgca
      121 tgtgccacag ggcctgcatc ccgatgcggc gcagtgcgcc agccatggat tgaattatgg
      181 gccgttgctg ccgtctgaag gtgtggtttg gcgagcagcg gccgcggccg tgccaacgtg
      241 attcgccaca ttcagatgag gtcagcacgc gccgaccgaa actggcccat cgtcgccacc
      301 ctgcaggctc acagccgcac cgggcgctgg cacgacccac gagatggatc cgtgcgcgcc
      361 gaatatgaac gcggcaagac gacgagcccg accggccccg acgggcagga gcgctacgcg
      421 gtcagggcgt agacccgatc ccggacctcc tcgaggtcgc cctcgggagt ggacgtgccg
      481 cccgtgatcc cgatgcgcgg cttgtcgcgc agccactccc cccagtcggc ggccagctcg
      541 tccgcatcgg ccacgtggat ggtctccggg cagatctcgc ggcacttgac cgccagctcc
      601 ttggtgttcg aagagttctt gccgccgacg accaccatgg cgtcgacctc ggccgcgagc
      661 ttcacgcttt cgtcctggcg ggtcagggtc accgggcaca cggtgttgac cactcgcagg
      721 tcatggcatc ggcgcaggca gaaggcggcg aactcctcca gcttgtgggg caggatggtc
      781 gactgcgaca gcacgcccag cttcttggtg cgtggcacgc gctcccaggt ggagcggtcc
      841 atgtcgtcca ccaccacgtg cttctggccg gcataggaac gcacgccctt gacctcgggg
      901 tggtcctcgt gaccgatgat gcacaccgtg tagccggcct ccgccagctc ctgcgcggcc
      961 ttctgcgact ggagcaccca ggtgcaggtg gcgtcgatga cctccagctc gccgccgccc
     1021 tccgccttgg cgcgcagctg ctccatctcg gtggcgggca cgccgtgggc gcggatcacc
     1081 agggtgccac cctcgacctg gtcggccagg tcggcggttg cgatgccgcg ttcggccagg
     1141 tcggccttga tgccagggtt gtggaccagc tggccgagcg tggtgaccgg cttgccctcg
     1201 tcgcgcgcct tcttggcctt gtcgatggcc aggcgcacgc cgtagcagaa gccggcgcgc
     1261 tccgcgattc ggacctcggg tcgtgtgggc gagtgaacag ccatcgatat gagtgtatcg
     1321 agatcgagcc gctcagtcag acgccccctc cggctcgacc gccccggcct cgccgtcctg
     1381 ccaggtccgc cgtcgtgcgg ggagcacgac ctcgcgcatc aggacctggg tcacggccac
     1441 cacggggatg gcgatcagcg ccccaagcac gccggcgatc gtcccgccgg ccaccaccgc
     1501 cagcagcacg atggtcggcg agacctgaat cgccttcttc tgcacgaacg gcaccaggac
     1561 gtacccctcg acctgctgca ggagcaggat ccagccagcc atgagcagcc cctgcacggg
     1621 gctctggaca aaggcgaccg cgatgatggg agccccgccg atccacgggc caaccagcgg
     1681 gatggcctcg gtgaggaagg ccaggaaccc gagcgggagg gcatacggca cgccgatgat
     1741 gagcatgcca accacggcac cgacgccggt ggcggacatg atcaccagct ggccgaccac
     1801 gtacgagccc agcttggaca tcaccgtttc gccgatgccg cgcattcgct cgcgctgccc
     1861 gggcggaaag tagccgagga gggcattcca catggagccg ccgtcgagga tcaggaagat
     1921 gctgaggaag atgatcagca ccacgttgac cagcacgccg gccacgacca gcggcacgcg
     1981 aagcgcggat tggatgccgg gcagcacctg gtcgaggctg gagcggattc cctccatggc
     2041 gtcctggatc gccgacccgg ggaccagcgg gccgagcgtg gtcctcagcc agccgagcag
     2101 ggcctcgatc gtttgcggca ggttctcgaa cagggcgctg gcctgctcca ccagcggcgg
     2161 gacgatcagc cacaccccca gcgcggccac cgccagcacg accaggtaga cgaccaccac
     2221 cccgatcacg cgcggcagac cgaggtttgc gagacgttcc accggtccgc ggatggcggc
     2281 ggccagcagc agagccagga agaccaggga gagcaccacg cgcagctgcc agatgaggta
     2341 ggccgccagc acgatcgcca gcaggacgat gccccaggtc gcggcggtga gcgctgctcg
     2401 cctgacgctt ccctcgtccg ggtcagggaa gctcgactgc ttgggcggca tgcgaggcaa
     2461 ggcgcacgcc gtatgccagc tcagtcggcc gccgtccccc agcggccccg ctgctcttcc
     2521 ggcagcagcg ccgcgatctc gcgcatgatg cggtcagtcg ctgctcgcag ctcctggcgg
     2581 tccacctggt cggaggccgg cagctcgaac ggaaccccga tgcgaatggt cacctggccg
     2641 cgccgaggca accacgagcc cccggccaaa atgcgatgcg tcccggcgat ccccaccggc
     2701 aggaccggca cgccggtgcg cgtggccagc agcgccgctc ccaggcgacc ctccttcaaa
     2761 cggccgtccc ggctgcgggt gccctccggg aagacggcca gcggctcgcc ggcggccagc
     2821 agctcgagcg ccatgcgctg cgcggcacgg tcgcccgacc cgcggcgcac gaagaaggag
     2881 ccggcctggc cggcgagcca gccgatcacc ggccatcggc gcagctcgtc cttggccatg
     2941 aaatgcacca cccgcccggt acggtgcccc accacggagc ccaggaacgg cgggtccagg
     3001 ttggagcagt ggttggcgac caccacgaac gccccggagc gcggcacgtt ctccagcccc
     3061 tccacgcgtc cggggcccag cagccagagc agccagccgg tgatgatggc tcccggatgg
     3121 aagaaccagg tcctcatcgg tcctgcttgg cctggaccga tgcgcccgcc gcgcgcgccg
     3181 ggcgtccagg aaggtgctcc acgatggcct gcaggcaggc atccacgccg agctccccgg
     3241 tgtcgaggac cattgcgccc tccgccttgc ggaggggtgc caccgggcgg ccggagtcgg
     3301 cctcgtcacg ctgcctgatc tccgccaggt aggtctcgat tcgctcgggc cgtcccattt
     3361 cggccgcgcg tcgcgcggcg cgcacctcgg ccgtggcggt caggtacacc ttcagagtgg
     3421 cgtccgggag gaccacggtg ccgatgtcgc ggcccaccat gaccgtgtcc tgccgacgcg
     3481 ccgcggcccg ctggatgccc agcatcgcct ggcgcacggc cgcgtggcgg ctcacggcgc
     3541 tgaccaggcg gtccacgcgc gggcggcggg cggcgtcggt cacgtccttc ccgtcgagca
     3601 ggacggtctc gcgccgatcg gtctggtcgg gtcgcggccg ctcgacctcg atgcggacag
     3661 cgcgagccag cttggcgagc ctgtcctcgt cgtccgggtc gacgccggcc tgcaccgcgg
     3721 ccagggtcag cgcccggtac atgaggccgg tatccacgaa cgtggcaccg atgcgctccg
     3781 ccagcgcgaa gccgatcgtc gacttccccg atcctgacgg cccatcgatg gccacggcca
     3841 gccggccgcg ccgtcggcgc cccgggtcac cgggcgtggg cgacccgctg ccgaccaggg
     3901 cctgcacctc ggccgcgtcc agcacgcgcc actcgccggg ctcaagaccg tccagcgcga
     3961 gcggcccgaa tccgacgcgg accaggcgac ggacccgcag ccccaccgcc gccatcaggc
     4021 gccgcacctc gcgcttgcgc ccctccccca ggcgcacgcg gagccacgtc ccccgttccg
     4081 caggatcacg gtggacctcg cgaggtggcg cggcggggcg agcttccagc agccgtgccc
     4141 gaccgtcctc cagctgcacg ccgtccagta gcgccaccag ttggtcggga tggggcgcgc
     4201 gatcaaccag cacggcgtac tcgcgctcca tcccgtaccg agggtgcagc agccggttcg
     4261 cccaggcgcc gtcgttggtg agcaccatca gcccctcgga ttcgacgtcc agccgtccgg
     4321 ccggccacag gcgcacgccg gccgcctcgg gagcggcctc caccagccgc agcaccgaat
     4381 gacggccgcg ctcgtcgcgc gcggacgaga cgtagcctgc cggcttgtgc agcgcgaagt
     4441 ggatggtgcg ctcgaccggt cccacgcgcc ggccgtcgac catgatctcg tcggttgccg
     4501 ggtctgccga ctggccgagc tcggctggcc ggccgttgac gctcactcga ccggcggcga
     4561 tcagatcctc ggatgcacgc cgagaggcca cgccggcctg ggccaggacc ttctggagac
     4621 gctcagccgg catcgggcac cgacgtctcc gccgccagcg ccgccgccgc cgaggagagg
     4681 ggcggcaggt catcgagcga cgtcagcccg aaccgctcca ggaaggcgaa cgtggtcccg
     4741 aacagcaccg gccggccggg tgtgtcgcgc cgtccgacct cgacgataag acggcgatgc
     4801 agcagcgagc gaatgacgta atccgagtcc acgccgcgca cccgctccac gtccgccctg
     4861 gtgcacggct ggcggtaggc gatgaccgac agcgcctcca gggcagctgg tgacaggcgc
     4921 ccctcctcgc gcgccgcgta agctgccaac cgggcgccca cctccggggc ggtcaccagc
     4981 tgccaggcat cgtccatgcg ctgaacccgc agcccgcgcc cgtcggcctc gagccggtcg
     5041 gccagctcca ccagctcggc ctcggcctgc aggtggggca cgctcgccag ctcg
//
LOCUS       DATACA010000082         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2020274, whole genome shotgun sequence.
ACCESSION   DATACA010000082 DATACA010000000
VERSION     DATACA010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020274"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VHK28_01835"
     CDS             complement(<1..420)
                     /locus_tag="VHK28_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2141002.1"
                     /translation="MAVTRPARQLTLADALVPAAERRAAALLRDVALVAGGAALIAIA
                     AQGAFRMPWTEVPYTAQTGAVLLVGTALGWRRGLLSMVLYLLMGVAGAPVFSAGNHGL
                     GQLLGVTGGYLVGFVVAAALVGRLAERGWDRSPLRAAG"
     gene            556..1722
                     /locus_tag="VHK28_01840"
     CDS             556..1722
                     /locus_tag="VHK28_01840"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX2141003.1"
                     /translation="MHTLPQTAIPTPAADARPASFSSPGLLALEGGSIYRGRLRGARV
                     RAAGDLIFTTAATGWGEILTDPSYAGQMVVLTHPMAGSYRIDPSDLESSQVHARALIV
                     TRLVTPPPGPGLALEELLFEAGIPALEGIDTRALTLELRRGAARRAVTGPMTGDQHEE
                     ARAVEAARRSLAWESVDHVAAVASRVAHEVAPAGAVRGRATLVDYGVKRSLLAALSDR
                     GLLVTVLPPYASAADVAATDPDLVVLSPGPGDPSRMAPQIAMVSELASVAVGGGTPIL
                     GICLGHQLLALAAGAETRRLAVGHHGGNHAVLEVETGRVDIGAHNHEVAVLDSERLTA
                     AGYRVSHRDLNDRTVEGLVHATGRIASVQFHPEGAPGPVDAARVFDLALEGALA"
     gene            1719..2912
                     /locus_tag="VHK28_01845"
     CDS             1719..2912
                     /locus_tag="VHK28_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2141004.1"
                     /translation="MTGSLTLAPRDALRLAGLLALAAFAFLLVSGRSIDLLGSPARLG
                     LMLAWPAVAVAFWASGRAWPVVAYVIAGGLLMRWVELPPGGAGPSDHLAATYEAIDVY
                     LSGGNPYDHVYQLTRPPGSPVSQPPGELLLHLPGYLLGGLGGVQFTQFVLAALTMGGL
                     AWAAARFSWLAGLPALALYAGAPNLVLLATDGSNDTGVAALLLLAVLALAWAVSRGDQ
                     AALLTAGAAAAFAVSTKQLALPIIVALASVAVQQVGWRRGGSYLLGAAWLLLILSIPF
                     LLMGPVEFFSALLSFVGAHDDIYGWNLWAFVQGLGGTPWDSGAAAILTIIVGGAALVG
                     LVLVRWRSLTGAVLGGVIATLLVMFAARWTTYAYFGLVMPVAMALPLLFAWERRGLQA
                     NEAVA"
BASE COUNT          373 a         1119 c         1125 g          467 t
ORIGIN      
        1 ccccgcggcg cgcaggggcg accgatccca cccccgctcc gccagccggc cgaccagcgc
       61 cgcggcaacc acgaatccca ccaggtagcc gccggtgacg cccagcagct gccccagtcc
      121 atgattgccg gccgagaaga cgggtgcccc ggccacgccc atcaacaggt acagcaccat
      181 gctgagcagg ccgcgccgcc agcccagggc ggtgccgacc agcaggacgg cgccggtctg
      241 cgccgtgtac ggcacctccg tccacggcat ccggaaggcg ccctgcgcgg cgatcgcgat
      301 cagcgcggcg ccgccggcca ccagcgcgac gtcgcgcagc agggcggccg ctcgacgctc
      361 ggcggcgggg accagggcat cggccagggt cagctgccga gctggacgcg tgacggccat
      421 gggttcggta cctcctcgtt ggccaacgta gatggacgga tcgtagcggg ccggcgccac
      481 tcccagcgca cccggtgcgc tacactgcgc caggtccttt aagtccggca cagagaggcc
      541 ggaaaggagc gagacgtgca caccctgccg cagactgcca taccgacgcc agccgccgat
      601 gcgcggccgg cgtcgttttc atctccgggc ctgctggcgc tcgagggcgg cagcatctac
      661 cggggtaggc tgcgcggggc acgcgtgcgg gcggcggggg acctgatctt caccaccgcc
      721 gccaccggct ggggcgagat cctgaccgat ccttcctatg ccggccagat ggtggtgctc
      781 acccatccca tggccggcag ctaccggatc gacccctccg acctggagtc gtcgcaggtg
      841 catgcccgcg ccctgatcgt cacacggctg gtgacgccgc ctcctgggcc aggtctcgcc
      901 ctcgaggagc tcctgttcga ggcgggcatc ccggccctgg aggggatcga cacgcgggcg
      961 ctgaccctcg agctgcgacg gggtgcagcg cgccgcgccg tcacgggccc catgaccggc
     1021 gaccagcacg aggaggcgcg cgccgtcgag gccgcgcgac gcagtctggc ctgggagtcg
     1081 gtcgaccacg tcgcggccgt cgccagccgg gtggcgcacg aggtcgcgcc cgccggcgcc
     1141 gtgcgcggcc gcgccacgct ggtcgactac ggggtcaagc gctcgctgct ggccgcgctg
     1201 tccgatcgtg gactgctggt cacggtgctc cccccgtatg cgagcgcggc cgatgtcgcc
     1261 gccacggacc cggacctggt ggtcctgtcg cccgggccgg gcgatccgtc gcgcatggcg
     1321 ccgcagatcg cgatggtcag cgagctggcc tcggtggcgg tgggaggtgg gacgccgatc
     1381 ctcggcatct gcctcgggca tcagctgcta gcgctcgcgg ccggagccga gaccaggcgg
     1441 ctggcggtgg gccaccacgg gggtaaccac gcggttctcg aggtcgagac gggccgggtg
     1501 gacatcggag cccacaacca cgaggtggcc gtgctggact cggagcgatt gacggccgcc
     1561 ggctaccggg tcagccatcg ggacctgaac gaccggaccg tggaggggct ggtccacgcc
     1621 acgggccgaa tcgcatcggt ccagttccat ccggagggtg cgccgggacc ggtggatgcc
     1681 gcgcgcgtgt ttgacctggc cctggagggc gccctcgcgt gacggggagt ctcacgctgg
     1741 ctccacgtga cgcgctgcgc ctggccggcc tgctggccct ggcggccttc gcgttcctgc
     1801 tcgtctcggg ccgatcgatc gacctgctcg gctcacccgc tcgcctcggc ctgatgctgg
     1861 cctggcccgc cgtggccgtc gctttctggg cgtcgggccg cgcctggccg gtggtcgcct
     1921 acgtgatcgc cggcggcctg ctgatgcgct gggtcgagct gccaccggga ggtgcggggc
     1981 cgtcggacca cctggcggcc acctacgagg ccatcgacgt gtacctctcg ggcggcaacc
     2041 cgtacgacca cgtctaccag ctcacccgcc cgcccggctc accagtctcg caaccgccgg
     2101 gcgagctgct gctccacctg cccggctacc tgctcggcgg gctgggaggc gtccagttca
     2161 cgcagttcgt gctggccgcc ctcaccatgg gcgggctggc gtgggccgct gcgcggtttt
     2221 catggcttgc cggcctgccc gccctggcgc tctacgccgg cgctccgaac ctggtgctgc
     2281 tggccaccga tggctccaac gacaccggcg tggctgcact gctgctgctg gccgtcctgg
     2341 cgttggcctg ggccgtatcc cgcggcgacc aggccgccct gctgaccgcc ggcgccgccg
     2401 ccgcattcgc ggtctcgacc aagcagctcg ccctgccgat catcgtggcg ctggcctccg
     2461 tggcggtgca gcaggtcggc tggcgccgtg gcggttccta cctgctcggg gccgcctggc
     2521 tgctgctgat cctgtcgatc ccgttcctgc tgatgggccc ggtcgagttc ttctcggcgc
     2581 tgctgagctt cgtgggcgcg cacgacgaca tctacggctg gaacctgtgg gccttcgtgc
     2641 aggggctggg tggcacgcca tgggattcgg gggctgccgc aatcctgacc atcatcgtgg
     2701 gcggggcggc gctggtcggg ctggtcctgg ttcgctggcg ctcgttgacc ggcgccgttc
     2761 tcggtggcgt gatcgccacg ctgctggtga tgttcgccgc gcgctggacg acctacgcct
     2821 acttcgggct ggtgatgccg gtggccatgg cgctgccgct gctcttcgcc tgggagcgcc
     2881 gtggcctgca ggcgaatgag gccgtggcat gaaggcgccc agcaaggtcc tcgtcatcgg
     2941 atcgggcccg atcctcatcg ggcaggcggc cgagttcgac tacgccggcg tgcaggcctg
     3001 cctggcgctg cgagcggagg gcgtggagac ggtgctggtc aactcgaacc cggccacggt
     3061 gatgaccgac ccggacgtgg gagg
//
LOCUS       DATACA010000083         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2180799, whole genome shotgun sequence.
ACCESSION   DATACA010000083 DATACA010000000
VERSION     DATACA010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180799"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VHK28_01850"
     CDS             <1..240
                     /locus_tag="VHK28_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX2141005.1"
                     /translation="VGLSQPLISWHLRILRLAGLIETRRQGRETICQLRVAAFEELHE
                     GEARLIGGTAGIAGTEPAPTDVPKPTTRPEVPDVG"
     gene            230..919
                     /locus_tag="VHK28_01855"
     CDS             230..919
                     /locus_tag="VHK28_01855"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2141006.1"
                     /translation="MSASHTPTRDLRRGDSLLSDRTKEAGRAVLAPVVRLALRLGLTA
                     NGVTIIGFVIVSTAAVLIGTGNLLIGAAVLTAGSLLDAVDGALARASGGTTPFGGFLD
                     STLDRAAEAALYAGVVAYYLNTAEQPTGPVVLAFVALAGSFLVSYTRARAEAIGLTAS
                     VGLAPRVERLVLIVAGIALAGLGFGIGLIGALAVIAVLSVATTVQRIWHVQRLSAAPP
                     PAGGDTTREKQ"
     gene            921..2132
                     /locus_tag="VHK28_01860"
     CDS             921..2132
                     /locus_tag="VHK28_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX2141007.1"
                     /translation="MADANGKSATNGKRPSDGKVRVAIVGVGNCASSLVQGRYYYEDA
                     KPGDFIPGLMHVNLGGYHIRDIEFVAAFDVDRNKVGKDLSEAIYAEPNNTYKFHSVPN
                     LGVPVERGMTHDGLGKYLSEIIEKAPGPTADIVGILRERQVDVMVSYLPVGSEDATKW
                     YAEQAIQAGVAFVNAIPVFIGREPYWQRRFAEAGLPVIGDDIKSQVGATITHRVLTRL
                     FMDRGVRIDRTYQLNFGGNTDFLNMLERERLESKKISKTNAVTSMIDYEIADDDVHVG
                     PSDYVPWLKDRKWCHIRMEGTTYGDVPLNLELKLEVWDSPNSAGVITDAIRCAKLGLD
                     RGLAGTLVAPSSYFMKSPPLQIHDDVAHQRVEAFIRGDDDETLVGTEQPRKREMRKLA
                     GSRTRTASASR"
     gene            2139..>3122
                     /locus_tag="VHK28_01865"
     CDS             2139..>3122
                     /locus_tag="VHK28_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2141008.1"
                     /translation="MQPETAEPVSRSAWSRISRPSAGGGERGRELLAYWAYRAAERLI
                     SALPRAVVLPIAAAAGNLAYDLGGDKRRVVAENLAPAMGLPPDDRRVARATRRAFRNY
                     ARYLADVMRLAGLTPQEVAALVDIDNLELLHQAHAEEKGVLLCTLHVGGMDLIASGLS
                     LQEQELHVIADDTTYGRLYEHLRAVRARHGLTLIGWRNLRGMVRALRSGGNLVLFCDG
                     GYRAGDVPVEFLGEATTFPAGPATLSARSGAPMLPVACQRTRSDRFRAHGLPLVRAER
                     DDPAEIYRATQALADELAGVIASDPGQWYMFRPVWPQTDEDRARARAALERA"
BASE COUNT          484 a         1128 c         1072 g          438 t
ORIGIN      
        1 gtcggcctgt cgcagccgct catcagctgg cacctgcgca tcctgcgcct agccggactg
       61 atcgagaccc gccgccaggg tcgcgagacg atctgccagc tgcgggttgc agcattcgag
      121 gagctccacg agggcgaggc gcgcctgatc ggtggcacgg cggggattgc cggcacggaa
      181 ccggcaccca ctgacgtgcc aaagcccacc acacggccgg aggtcccaga tgtcggctag
      241 ccacacgccc acccgcgacc tgcgccgcgg cgactcgctg ctctcggacc ggaccaagga
      301 ggccgggcgc gccgtgctgg ctcccgtcgt gcggctggcc ctgcggctgg gactcaccgc
      361 caacggcgtg acgatcatcg ggttcgtgat cgtgtcgacc gccgccgtgc tgatcggcac
      421 cggcaacctg ctcatcggcg ccgcagtcct gaccgccggt tcgctgctgg atgccgtgga
      481 tggcgcgctg gcgcgggcca gcggcggcac gaccccgttc ggcggcttcc tggactcgac
      541 cctggaccgc gccgcggagg cagccctcta cgccggcgtg gtggcctact acctgaacac
      601 ggccgagcag cccaccggcc cggtggtgct cgccttcgtg gccctggccg gctccttcct
      661 ggtgagctac acgcgcgcca gggcggaggc catcggcctc accgccagcg tggggctcgc
      721 gccccgcgtt gagcgactcg tcctgatcgt ggccggcatt gcccttgcgg gcctcggctt
      781 cggaatcggg ctcatcggtg ccctggcagt gatcgccgtc ctctcggtgg cgaccaccgt
      841 ccagcggatc tggcacgtcc agcggctcag cgcggccccg ccgcccgccg gtggcgacac
      901 gacaagggag aagcaatagc gtggctgacg cgaacggcaa gagcgcgacg aacggaaaac
      961 gcccctcgga cggcaaggtc cgggtggcga tcgtgggcgt gggcaactgc gcctcgagcc
     1021 tggtccaggg acgctactac tacgaggacg caaagcccgg cgacttcatc ccggggctga
     1081 tgcacgtcaa cctgggcggc taccacatcc gcgacatcga gttcgtggcg gccttcgacg
     1141 tcgaccgcaa caaggtcggc aaggacctgt cggaggcgat ctacgccgag ccgaacaaca
     1201 cctacaagtt ccactcggtg ccgaacctcg gcgtgccggt ggagcgcggc atgacccacg
     1261 acgggctggg caagtacctc tccgagatca tcgaaaaggc cccgggaccc accgccgaca
     1321 tcgttggcat cctgcgcgag cgccaggtgg acgtcatggt ttcctatttg ccagtcggca
     1381 gcgaggacgc caccaagtgg tacgccgagc aggccatcca ggcgggggtg gccttcgtca
     1441 acgccatccc ggtcttcatc ggtcgtgagc cctactggca gcgccgcttc gccgaggccg
     1501 ggctgccggt catcggtgac gacatcaaga gccaggtggg agccaccatc acccatcggg
     1561 tcctgactcg gctgttcatg gaccgcggcg tgcggatcga ccgaacctac cagctgaact
     1621 tcggcggcaa caccgacttc ctcaacatgc tcgagcgcga gcggctggag tccaagaaga
     1681 tcagcaagac caacgcggtc accagcatga tcgactacga gatcgccgat gacgacgtgc
     1741 acgtcggccc ctcggactac gtgccatggc tcaaggaccg caagtggtgc cacatccgca
     1801 tggagggcac cacctacggt gacgtgccgc tcaacctgga gctcaagctg gaggtctggg
     1861 actcgccgaa cagcgccggg gtgatcaccg atgccatccg ctgcgccaag ctgggcctgg
     1921 atcgcgggct ggccggcacc ctggtcgcgc cgagcagcta cttcatgaag tcgccgccct
     1981 tgcagatcca cgacgacgtc gcgcaccagc gggtggaggc cttcatccgc ggcgacgacg
     2041 acgagacgct ggtgggcacc gagcagccgc gcaagcggga gatgcgcaag ctggccggat
     2101 ccaggaccag gacggcctcc gccagccgct gatcgggagt gcaacccgaa accgccgagc
     2161 cggtcagccg cagcgcctgg tcgcgcatct cgcgtccatc cgccggcggc ggcgagcgcg
     2221 ggcgcgagct gctcgcctac tgggcctacc gggcagcgga gcggctgatc agcgccctgc
     2281 cgcgggccgt ggtgctgccc atcgcggccg ccgccggcaa ccttgcctat gacctgggcg
     2341 gggacaagcg ccgggtggtc gccgagaacc tggcgcccgc catgggcctg ccgccggacg
     2401 atcgccgggt ggcccgcgcc acgcggcgtg ccttccgcaa ctacgcgcgc tacctggccg
     2461 acgtcatgcg gctggcggga ctcacgccgc aggaggtcgc ggcgctggtc gacatcgaca
     2521 acctcgagct cctgcaccag gcgcatgccg aggagaaggg cgtcctgctc tgtaccctgc
     2581 acgtgggagg catggacctg atcgcctcgg gactctcgct gcaggagcag gagctgcacg
     2641 tgatcgccga cgacacgacc tacggccgcc tgtacgagca cctgcgcgcg gtgcgcgccc
     2701 ggcacggcct gacactgatc ggctggcgca atttgcgtgg catggtccgc gccctgcgct
     2761 cgggcggcaa cctggtcctc ttctgcgacg gcggctaccg ggccggcgac gtgccggtcg
     2821 agttcctggg cgaggccacc accttcccgg ccgggccggc caccctctcg gcacgctcgg
     2881 gtgcaccgat gcttcccgtg gcctgccagc ggacccgcag cgaccgcttt cgggcgcacg
     2941 gcctccccct ggtgcgggcg gagcgcgacg accccgccga gatctaccgc gccacccagg
     3001 cgctcgccga cgagctggcc ggggtgatcg cctcggatcc cggccagtgg tacatgttcc
     3061 gacccgtctg gccccagacc gatgaggacc gcgctcgcgc gcgggcggcg ctggagcgcg
     3121 cg
//
LOCUS       DATACA010000084         4340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1137237, whole genome shotgun sequence.
ACCESSION   DATACA010000084 DATACA010000000
VERSION     DATACA010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137237"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHK28_01870"
     CDS             <1..220
                     /locus_tag="VHK28_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX2141009.1"
                     /translation="SAHADMVNSGTRDASLIKSAVFLSEFATVPWVHVDIAGSAYLTA
                     DKPANPKGALGTAVSTLVKLALNYRDGA"
     gene            complement(287..490)
                     /locus_tag="VHK28_01875"
     CDS             complement(287..490)
                     /locus_tag="VHK28_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX2141010.1"
                     /translation="MDKDRIEGGVKEGVGKAKEGWGDLTDDASTEAEGHRDQAEGELQ
                     QGWGETKDAARDLTDDVEDDGPL"
     gene            626..1285
                     /locus_tag="VHK28_01880"
     CDS             626..1285
                     /locus_tag="VHK28_01880"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX2141011.1"
                     /translation="MTPLALAVGASFALFGAAAERLASVWPAEEASRRAPGLRTVLLA
                     LAAGAAGWGIAARSTLPAWATAVHLAILALLVVLSATDLDQRRLPHIVLDPLIVVAVA
                     FVPFNPSVPPLDAVIGATVAVAFLGVLNLLIRGGVALGDLYLVAPLGLLLGWEGVFGA
                     IFLAALLSAITSVILLATRRAGLKSYIPFGPFLVAGAVLALLRDDHLLSEAAAAAMRL
                     L"
     gene            1539..2837
                     /locus_tag="VHK28_01885"
     CDS             1539..2837
                     /locus_tag="VHK28_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX2141012.1"
                     /translation="MSTLLRRALALVLLVATVAVLAPGSVRQTVRADDPNVNDAIRAQ
                     QQMEAELARQRAQLADLRREQSELTASLAKLETDLGRVGLELAEAQEQLQRVTTTLEK
                     SRADLERYRIQISNLEANLEEIAVDIRETRQELAARELLLQDHLRAAYEQSQTSVLEI
                     LLSTESFTDASSQLSYLLTLSDEDRLLAEDIRTTRERLQVRRKTLRDGRETLAALRDQ
                     EAERAASLEEQQAIVDAARARLAEHQARLAELQVEQEAQLAAAQRGERQTAEQIAAQQ
                     RALAGQQELVERLKEQAARLDIAYRGRFAWPQRGDFYVTQEFGHTTFNPKHTGIDMAY
                     STPRCGGPIYAAADGTVLADGRPNAKYGDTAIGVVIGHSQRLQTWYWHLSSEQVSVGQ
                     EVSTGDLIGYEGATGIATGCHLHFETLLDGTPQNPRNYLP"
     gene            2966..>4340
                     /locus_tag="VHK28_01890"
     CDS             2966..>4340
                     /locus_tag="VHK28_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2141013.1"
                     /translation="MQRRRHRLMARRTTRRGSQRRGLATAFIVALGVFALLVVGSVAG
                     TAGGLLAAYSYFASGLPDPRLLDDIELPASTYVYDRTGNTLLARFECQNRDQVSFEEL
                     PDHVVNATVAAEDRTFWTNDGVDYYAVAAAAVANLEAGEIVRGASTITAQVIKYAGSI
                     KEAEEQDRPGSTAAPTTALDPEIETSDPELEACEPPDLTFLSGRGYDDKVREFILARQ
                     MTDAYPGRQGKEQILETYLNLIFYGNGSYGIQAAAANYFGISDLTKLSLAQSAFLAGL
                     PQLPSAYDPYFNNQGPARAIARRNVVLDAMLRDGYITPAEHDQASATTWEEMQPKQIS
                     AILREPHFSLRVSREAERIFEAMGKANPAQEVRTGGYRITTTLDYGLQDVAKDQVTAW
                     VNHPELKNKNVNNGALVLGHILQPIVQGGRDPVAAGADLLRRVGLAHGLEDTLSLAAH
                     AKAEVRFP"
BASE COUNT          669 a         1610 c         1399 g          662 t
ORIGIN      
        1 ctccgcccac gctgacatgg tcaactccgg cacgcgcgac gcgtcgctca tcaagagcgc
       61 cgtcttcctg tcggagttcg ccaccgttcc ctgggtgcac gtggacatcg ccggcagcgc
      121 ctacctgacc gccgacaagc cagccaatcc caagggcgcg ctgggtactg ccgtctcgac
      181 gctcgtcaag ctggcgctga actaccgcga cggcgcatag cctcaggctg gggaagggcc
      241 ccaccgcggc ggcgatgggg tccttctctc gtggtatgga cggggcctac agcgggccgt
      301 cgtcctcgac gtcgtccgtc aaatcgcggg cagcgtcctt ggtttcgccc cagccctgct
      361 ggagctcgcc ctcggcctgg tcccggtgtc cctcggcttc ggtcgaggcg tcatcggtca
      421 ggtcgcccca accttccttg gccttgccga cgccttcctt gacgccgccc tcgatgcgat
      481 ccttgtccat ctcgtgcctc cttgctgcac gctgcgaccg gctcccccgc cggcgcagcc
      541 gagcgcgcgg ccggtcgctg gccccgagtg gcaggttgcg tgccatcctg cccctccggc
      601 gtggaccggc ggctaggatg cgcccgtgac gccactcgcc cttgccgttg gagccagttt
      661 cgccctgttc ggcgctgcag ccgagcgact ggcgtccgtc tggcctgccg aggaggcgtc
      721 tcggcgtgcg cccggtctcc gcaccgtgct gctggccctc gccgctggag ccgcgggatg
      781 gggaatcgcg gcccgctcga cgctgcccgc ctgggccacg gcagtccatc tggccatcct
      841 ggccctgctg gtggtgctat ccgccaccga cctggatcag cgtcgcctgc cgcacatcgt
      901 gcttgaccca ctgatcgtgg tcgccgttgc cttcgtgccc ttcaacccca gcgttccgcc
      961 gctcgacgcg gtcatcgggg cgaccgtcgc agtggctttc ctcggcgtgc tcaacctgct
     1021 catccgcggc ggagtggcac tcggcgacct ctacctcgtt gcgccgctgg gcctgctgct
     1081 gggctgggag ggcgtcttcg gcgcgatctt cctggccgcc ctgctctcgg ccatcaccag
     1141 cgtgatcctg ctcgctacgc gtcgcgccgg cctgaagagc tacatcccgt tcgggccgtt
     1201 cctggtggcc ggcgcggtgc tggccctgct tcgcgacgac cacctgttga gtgaggcggc
     1261 agccgccgcc atgcgcctcc tctgaggcac cgcccggccc agttctggac tttcacggct
     1321 cggtttcgat ggcgccgcga ggggcgaggt cgtgcaatag cccgaacgtc agggccaagg
     1381 ggactggcgc aggactggac ttctccgtta tccttcgccg gcgcccaagc gacccgagac
     1441 ccagcattgc cccgcgtccc acagctggac gtgccctgta cgcggtgagc gcaggccagc
     1501 cagcgctccg gcgcgcagaa ccatcacagc aggagctcat gtcgaccctc ctccgccgag
     1561 cactcgccct cgtcctcctg gtggccaccg tcgccgtcct cgctcccggc agcgtgcggc
     1621 agaccgttcg tgcggacgat cccaacgtca acgatgccat ccgcgcacag cagcagatgg
     1681 aggccgagct cgcccgtcag cgtgcgcagc tcgctgacct gcgccgcgag cagtcggagc
     1741 tgaccgccag tttggccaag ctggagaccg acctcggccg cgtcggactg gagctggcgg
     1801 aggcgcagga acagctgcag cgggtgacca ccacgctcga gaagtcgcgc gccgacctcg
     1861 agcgctaccg gatccagatc tccaacctgg aagccaacct ggaggagatc gcggtcgaca
     1921 tccgcgaaac acgccaggag ctcgccgctc gcgagctgtt gctgcaggac cacctgcggg
     1981 ccgcctacga gcagtcccag acgtcagtcc tggagatcct gctctccacc gagtccttca
     2041 ccgacgcatc cagccagctc tcctacctgc tcaccctgtc cgacgaggac cggctgctgg
     2101 ccgaggacat ccgcaccacc cgcgagcgcc tgcaggttcg ccgcaagacg ctgcgcgacg
     2161 gtcgcgagac gctggcggcg ctgcgtgacc aggaggccga gcgggccgca tcgctcgaag
     2221 agcagcaggc catcgtcgac gcggcacggg cgcggctggc cgagcatcag gcgcgcctgg
     2281 cggagctcca ggtcgagcag gaggctcagt tggcggccgc ccagcgcgga gagcgccaga
     2341 ccgccgagca gattgcagcc cagcagcgcg cgctggccgg ccagcaggag ctcgtggagc
     2401 ggctcaagga acaggccgcc cggctggaca tcgcataccg gggtcgcttc gcctggcccc
     2461 agcgcgggga cttctacgtc acccaggagt tcggccacac cacgttcaac ccgaaacaca
     2521 ccggaatcga catggcctac tccacgccgc gctgcggcgg gccgatctac gccgcggccg
     2581 acgggaccgt gctcgccgac ggccggccga acgccaagta cggcgacacc gccatcggcg
     2641 tggtcatcgg tcattcgcag cgcctgcaga cctggtactg gcacctttcc tccgagcagg
     2701 tgagcgtagg ccaggaggtg agcaccggcg acctcatcgg ctacgaaggt gccacgggaa
     2761 tcgccaccgg ctgccacctg cacttcgaga cgctgctgga cggaacgccg cagaacccgc
     2821 gcaactacct gccctgacgc ctccgacgcc tggcgcgccc gagctcgagg caccggtcgt
     2881 gcaggagcaa cacggagacg acctcggccg tcattcgacc ggacaacgac gcatgcggta
     2941 ccgttagcca cagatgcacc ctgccatgca gcggcggcgc catcgtttga tggcgcgccg
     3001 cacgacccgc cgcggctccc agcggcgcgg gcttgccact gccttcatcg tcgcgctggg
     3061 cgtcttcgcc ctgctggtcg tcggatcggt ggcgggcacc gccggcgggc tgctggcggc
     3121 ctacagctac ttcgccagtg gcctacccga cccgcggctg ctcgacgaca tcgagctgcc
     3181 ggcgtccacc tacgtctacg accggaccgg gaacaccctg ctcgcccgct tcgagtgcca
     3241 gaaccgtgat caggtctcct tcgaggagct gccggatcac gtggtgaacg ccacggtcgc
     3301 cgccgaggac cgcaccttct ggaccaacga cggcgtggac tactatgcgg tcgccgccgc
     3361 ggccgtcgcg aacctggagg ccggcgagat cgtgcggggg gccagcacca tcaccgcgca
     3421 ggtcatcaag tacgccggct cgatcaagga ggcggaggag caggaccgcc cgggaagcac
     3481 cgcggcaccc accaccgcac tcgatccgga gatcgagacg agcgatccgg agctggaggc
     3541 ctgcgagccc ccggacctca ccttcctctc gggtcgaggt tatgacgaca aggtccgcga
     3601 gttcatcctg gcccgccaga tgaccgatgc ctaccctggg cgccagggca aggagcagat
     3661 ccttgagacc tacctgaacc tgatcttcta cgggaacggc tcgtacggca tccaggcggc
     3721 cgccgccaac tacttcggca tctccgacct gaccaagctg agcctggcgc agtccgcgtt
     3781 cctggccggc ctgccgcagc tgccgtccgc ctacgacccc tacttcaaca accagggtcc
     3841 ggcgcgggcc atcgcgcgcc gcaacgtggt actggacgcc atgctgcgcg atggctacat
     3901 cacccccgcc gagcacgacc aggcgtccgc caccacctgg gaggagatgc agccaaagca
     3961 gatcagcgcc atcctgcggg agcctcactt cagccttcgc gtgagccgcg aggctgagcg
     4021 catcttcgag gccatgggca aggccaaccc ggcgcaggag gtccgcaccg gcggctaccg
     4081 gatcacgacc accctggact atggcctgca ggatgtggcc aaggaccagg tgaccgcctg
     4141 ggtgaaccat ccggagctga agaacaagaa cgtcaacaac ggggccctgg tccttggcca
     4201 catcctgcag cccatagtcc agggtggtcg tgatccggta gccgccggtg cggacctcct
     4261 gcgccgggtt ggccttgccc atggcctcga agatacgctc agcctcgcgg ctcacgcgaa
     4321 ggctgaagtg cggttcccgc
//
LOCUS       DATACA010000085         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1618692, whole genome shotgun sequence.
ACCESSION   DATACA010000085 DATACA010000000
VERSION     DATACA010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618692"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VHK28_01895"
     CDS             complement(<1..244)
                     /locus_tag="VHK28_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2141014.1"
                     /translation="MLVPLSWLREYVDVVLSPSELAEQLTLRGMEVQGIEVSGAGWTD
                     VVVGRLLSVDRHPNADKLWLTTVDVGGQEPLQVVCGA"
     gene            complement(248..1330)
                     /gene="pheS"
                     /locus_tag="VHK28_01900"
     CDS             complement(248..1330)
                     /gene="pheS"
                     /locus_tag="VHK28_01900"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2141015.1"
                     /translation="MTDLADRLEQLRADARRRLEAAVNAAAVEELRVALSGRSGKLTA
                     LLRELGSVTPEDRPRIGQMANQVRTEVEESIRQRLEALSTAALESQLEAERLDMTAPG
                     RPVRIGHLHPLMNVERDFREIFHAFGFEVFEGPEVETDWWNFEALNIPPDHPSRDLWD
                     TLYVAEPGSVEAGEPRPADDGTILRTHTSPVQIRAMRALDPPIRVIMPGRCYRYENVA
                     ADRNFEFFQIEVLVVDRDTSLADLKGMLEEFARVLYGPETPTRFRPGYYPFTEPSVAF
                     DIRCQVCGGPGCAACKRSGWVTILGAGMVHPTVIRNGGYDPSEYQGYAVGFGMDRLAM
                     NREGIADIRALLGADLRFLEQFSGGR"
     gene            complement(1546..1905)
                     /gene="rplT"
                     /locus_tag="VHK28_01905"
     CDS             complement(1546..1905)
                     /gene="rplT"
                     /locus_tag="VHK28_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016854830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX2141016.1"
                     /translation="MARVKRGVAAHKRHKRLLAQAAGRRGTRSKLVRPAREALIHALV
                     YAYRDRKVRKRDMRRLWIERINAASRQHGLPYGRFIAGLKSAGVEVDRKVLADLAVRE
                     PAAFAVMVETAKGSFTT"
     gene            complement(1916..2113)
                     /gene="rpmI"
                     /locus_tag="VHK28_01910"
     CDS             complement(1916..2113)
                     /gene="rpmI"
                     /locus_tag="VHK28_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX2141017.1"
                     /translation="MPKIKSHKGTAKRYKKTGAGKWVRPKAWRGHHLEIKSSRRTRRY
                     AGKAVVSGTHTKQLQRLLPYE"
     gene            complement(2114..>2771)
                     /gene="infC"
                     /locus_tag="VHK28_01915"
     CDS             complement(2114..>2771)
                     /gene="infC"
                     /locus_tag="VHK28_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX2141018.1"
                     /translation="EVRVIDDEGQQLGVMPTFKALALAQEKGLDLVEVAPTAVPPVCR
                     ILDYGQYKYELQKREREAKKKQKSQTFKEIRLRVKIDVNDLRTKLRRAAGFLDEGDRV
                     KVTVQFRGREMSHANLGRDLLERAAELLAEHGTVERPPLLEGRNMYIVMAPLEKRPEK
                     KTEQGQGRPAAAQPQPKSPSEGEETIGDALAAKGQAPTATVEPEPPAEGSEPAKQENK
                     "
BASE COUNT          375 a          974 c          940 g          482 t
ORIGIN      
        1 ccgctccgca caccacctgc agcggctcct ggccgccgac gtccacggtg gtcagccaca
       61 gcttgtccgc gttcgggtga cggtccacgc tgagcagccg ccctacgacg acatcggtcc
      121 atccggctcc tgagacctcg atgccctgga cttccatgcc ccgcagcgtg agctgctccg
      181 ccagctcgga cggcgacaga acgacatcca cgtactcgcg caaccaggag agcggcacca
      241 gcatctgtca gcgccctccg ctgaactgct cgaggaagcg caggtccgcg cccagcagcg
      301 cgcggatgtc cgcgatgccc tcccggttca tggccagccg gtccatgccg aacccgaccg
      361 cgtagccctg gtactcggac gggtcatagc cgccgttgcg gatcacggtg ggatgcacca
      421 tgccggcgcc gaggatggtg acccagccac tccgcttgca ggcggcgcag ccgggcccgc
      481 cgcacacctg gcagcgaatg tcgaacgcca cgctgggctc ggtgaacggg tagtagccgg
      541 ggcggaagcg ggtgggcgtc tccggcccat acagcacgcg cgcgaactct tcgagcatgc
      601 ccttgaggtc ggccaggctc gtgtcgcggt cgacgaccag cacctcgatc tggaagaact
      661 cgaagttgcg gtcggcggcc acgttctcgt agcggtagca gcggccgggc atgatgaccc
      721 ggatgggcgg gtccagggcg cgcatggccc ggatctgcac cgggctggtg tgggtgcgca
      781 aaatggtccc gtcatcggcc gggcgtggct ccccggcttc cacgctgccc ggctcggcca
      841 cgtagagcgt gtcccacaga tcgcggctcg gatgatccgg cgggatgttg agcgcctcga
      901 agttccacca gtcggtctcc acctccgggc cctcgaagac ctcgaagcca aaggcgtgga
      961 agatctcgcg gaagtcgcgc tcgacgttca tgagcgggtg caggtgaccg atgcgcaccg
     1021 gccgaccggg cgccgtcatg tccagccgct cggcctccag ttgtgactcc agggccgcgg
     1081 ttgacagggc ctccaggcgc tgacgtatgg actcctcgac ctccgtccgc acctggttgg
     1141 ccatttggcc gatcctgggg cggtcctcgg gcgtcacgga gcccagctcc cgcagcaggg
     1201 cggtcagctt cccgctgcgc ccgctgagcg cgacgcgcag ctcctcgacg gccgcggcgt
     1261 tgacggcagc ctccagccgc cgtcgcgcgt cggcgcgcag ctgctccagc cgatcggcca
     1321 ggtccgtcac ggcaagaccc cgttgctgtc ggcacccgcc tgtgtagcac ggtcgcacgg
     1381 tcgcacgggc gcacagccgc acaactggcc gtgatgtggc tccatgggtg gcgtgggcgt
     1441 gctctcgatc ttcatcggtc cgttgactgc aatcatcgcg ataaacgaac gaccccgctc
     1501 cgcaatggag cagggccgag gggccggcat cggtcttgac gccggctagg tggtaaagga
     1561 gcccttggcg gtttcgacca tgacggcgaa ggcggccggc tcgcgcacgg cgaggtcggc
     1621 gagcaccttg cggtccacct caacgcctgc cgacttcagc cccgcgatga aacgcccgta
     1681 tggcaggccg tgctggcggg acgcggcatt gatgcgctcg atccacaggc ggcgcatgtc
     1741 gcgcttccgg accttacggt cacggtaggc gtagaccagc gcgtggatga gcgcctcgcg
     1801 agcggggcgg accagcttgg agcgggtgcc gcgtcgcccg gccgcctgcg ccaggagccg
     1861 cttgtggcgc ttatgggcgg cgacgccgcg cttgactcgt gccatctggg gctccctact
     1921 cgtacggcag cagccgctgg agctgcttgg tgtgcgttcc ggagacgacg gctttgccgg
     1981 cgtagcgccg cgtgcggcgg ctggacttga tctccaggtg gtggccgcgc caggccttgg
     2041 gccggaccca cttgcctgca ccggtcttct tgtagcgctt ggccgtgccc ttgtgactct
     2101 tgatcttcgg catctacttg ttttcctgct tcgccggctc ggatccctcc gcggggggct
     2161 cgggctcgac cgtggcggtc ggtgcctgac ccttggcggc gagcgcgtcg ccgatcgtct
     2221 cttcgccctc cgagggcgat ttcggctggg gctgcgcggc cgctggtcgg ccctgcccct
     2281 gctcggtctt cttctccggg cgcttctcga gcggcgccat cacgatgtac atgttgcggc
     2341 cctcgagcag cggtggccgc tcgacggtcc cgtgctccgc caggagctcg gctgcgcgct
     2401 cgaggaggtc acgccccagg ttggcgtggc tcatctcgcg cccgcggaac tggaccgtga
     2461 ccttgacgcg gtcaccctcg tccaggaacc cggccgcgcg ccgcagcttg gtacgcaggt
     2521 cgttgacgtc gatcttgacc cggagccgga tctccttgaa cgtctgcgac ttctgcttct
     2581 tcttcgcttc gcgttcccgc ttctggagct cgtacttgta ctggccgtag tccaggatgc
     2641 gacagaccgg tgggaccgcg gtgggcgcca cctccacgag gtcgagcccc ttctcctgtg
     2701 ccagagccag tgccttgaac gtgggcatca cgccgagctg ctggccctca tcgtcgatga
     2761 cccgcacctc g
//
LOCUS       DATACA010000086         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_574781, whole genome shotgun sequence.
ACCESSION   DATACA010000086 DATACA010000000
VERSION     DATACA010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574781"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VHK28_01920"
     CDS             <1..1893
                     /locus_tag="VHK28_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX2141019.1"
                     /translation="AFIAAYTLYRHHRVLDAILLIGAFLVANLASTLTDLLGYLVLFM
                     LAALLLWLRAALIGREEGWQRRRVTENTEVPRAIMRSGVAFIAASIAMAWVLTSVAVA
                     SPLTAVWNNLDVVWHDVRDQLDGVFGGLSNADSRIGGTVFGSGFNVRGEWFSNDDPVL
                     TYAASQPYYLKTVTYDVYTGGGMESSDAESRSVAPGELIFPEDSPEQALNPESVEAET
                     IEVSIQGTVGRNLFSHGHPTVAYTPLIVGEPEGTSLLASLRSANAIDSGQGYAITALV
                     SNATRAQLASAGTQYPSQIAERYLSLDGVSGRTRQLAADIIAAAGAQNPYTRAEALAT
                     WLRTNPEFSYNESAPVPEDPQQDLVDFFLFDSKVGYCEYYAPAMALMARSVGLPARVA
                     GGFAPGEEIELPGGLGGGVFQIRERNAHVWTEIYFPGYGWQIFESTKSIAPVIRVAGE
                     PLASGGPAGPQASLPPLFDPGDPGVVNNLPSFDPIEGGFRPGDEAPPADATGGNTLLI
                     LGIGLALLLVIAWRWRKSRRLMRLLAPGERQWRRLTLAADRAGVTQRPSETIYEYAGW
                     LEEQIPARRGEIRTIARGKVWQSYSGHALTTDALARIEAAWKRLELPLMWLTIRRWFR
                     ALLPGR"
     gene            complement(1909..2604)
                     /locus_tag="VHK28_01925"
     CDS             complement(1909..2604)
                     /locus_tag="VHK28_01925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2141020.1"
                     /translation="MTARLLLVRHGITAWNREGRFQGHLDPPLADDGRKEAGLLAERL
                     ADGVDRPDRIVTSPLQRAATTADLIAGGLGDDAPNVEHDPRLMEIGQGEWEGRTHAEL
                     AVEDAERYAAWRQHSDQAQPPGAETIEAVLARVAELLHELAAAPGDVTCVVSHGGIIR
                     LAARHLLGLGAIRSWAMDVDNASLSVLTATAPAPATGSGSAWRVERWNDTAHLLGRAP
                     LHVDESEGEPLAL"
     gene            complement(2601..3758)
                     /locus_tag="VHK28_01930"
     CDS             complement(2601..3758)
                     /locus_tag="VHK28_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX2141021.1"
                     /translation="MDQNLRTPGPIPLPREVRHAQAQPMVDHRGTEFGEMQREITAGI
                     GQLIGTRGDVLLLTGSGTGSLEAAVVNTLSPGDRVLAVSIGSFGDRFAAIATAFGTDV
                     ERLEVEWGTAADPTVLEQHLASRPPFRAVLLTHNETSTGVANPLRELVAAVRAAPGEP
                     LAIVDGISGLGAIPFEMDAWGVDLALSASQKAWMGSPGIAIAAVGERARAAEASAAIP
                     RFYWDFAEARKWAEKGQTPWTPAVSVLFGLRVGVQRLRAEGRERTWARHAALAAAAGS
                     GLEALGLRLVAPPEHRSPTVTAAWLPDGLDWPPFNRDMRSRDLVVAGGQGKWVGRILR
                     FGHMGMVELDEMSDAVRVMGETLRDHGREVDPPAAVEAAREAFDRAAAAAR"
     gene            complement(3800..>4271)
                     /locus_tag="VHK28_01935"
     CDS             complement(3800..>4271)
                     /locus_tag="VHK28_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141022.1"
                     /translation="VAGEHPLWADAYIVGRVESVEREELEPITLVVTPTHAFGGDVPQ
                     RMRLAARSDGPPPPDSWRVGEHWFLGLEHAGAIEDVNATVAPCAPNFRVTTSDELDSL
                     VQAATTVEIRGVGASIAPAEAPPGTVAILGVSVVAMGIAAWVAFRRRSGRGENP"
BASE COUNT          601 a         1574 c         1481 g          615 t
ORIGIN      
        1 gccttcatcg cggcctacac gctgtaccgc caccatcggg tgctggacgc catcctgctg
       61 atcggcgcgt tcctggtcgc caacctggcc agcaccctga ccgatctcct cggctacctc
      121 gtgctcttca tgctggccgc cctcctgctc tggctgcgcg ccgcgctcat cggtcgggag
      181 gagggctggc agcgccgccg agtgaccgag aacaccgagg tgccgcgggc catcatgcgc
      241 agcggggtgg cgttcatcgc cgccagcatc gccatggcct gggtgctgac cagcgtggcg
      301 gtggcttcgc cgctgacggc agtctggaac aacctggacg tggtgtggca cgacgttcgg
      361 gaccagctgg acggcgtctt cggcggcctg tccaacgctg actcgcggat cggaggcacc
      421 gtcttcggct ccggcttcaa cgtccgcggg gagtggttca gcaacgacga cccggtgctc
      481 acctacgccg ccagccagcc gtactacctc aagacggtca cctacgacgt gtacaccggc
      541 ggcggcatgg agagctcgga cgcggagtcc cggtccgtgg cacccggcga gctcatcttt
      601 ccggaggaca gtcccgagca ggcgctgaac cccgagtccg tcgaggcgga gaccatcgag
      661 gtctcgatcc agggcaccgt cggtcgcaac ctgttcagcc acggccaccc gaccgtggcg
      721 tacacgcccc tcatcgtggg cgagccggag gggacctcgc tcctggcatc gctgcggtcg
      781 gcgaacgcca tcgattccgg ccagggctac gccatcaccg ccttggtgag caacgccacc
      841 cgcgcgcagc tggcctcggc cggcacgcag tacccgtcac agatcgccga gcggtacctg
      901 agcctggatg gcgtcagcgg acgcacccgg cagctggccg ccgacatcat tgcggcggcc
      961 ggggcccaga acccgtacac gagggccgag gcgctggcaa cctggctgcg caccaacccg
     1021 gagttcagct acaacgagtc ggcgcccgtg ccggaggacc cgcagcagga cctggtcgac
     1081 ttcttcctgt tcgacagcaa ggtcggttac tgcgagtact acgccccggc catggccctc
     1141 atggcccgct ccgtcggcct cccggcgcgc gtggccggcg gcttcgcccc gggcgaggag
     1201 atcgagctgc cgggcggcct gggcgggggc gtgttccaga tccgcgagcg caatgcgcac
     1261 gtctggaccg agatctactt ccccggctac ggatggcaga tcttcgagtc gaccaagagc
     1321 atcgcgccgg tcatccgcgt cgccggtgag ccgctggcgt ccggggggcc ggcaggtccg
     1381 caggcatccc tgccgccgct cttcgacccc ggggatccgg gcgtcgtgaa caacctgccg
     1441 tcgttcgacc cgatcgaggg tggattccgg cccggcgacg aggcgccgcc cgctgacgcc
     1501 accggtggaa acaccctgct gatcctgggc atcggcctgg cgctgctgct ggtgatcgcc
     1561 tggcgctggc gcaagtcgcg gcggctgatg cgcctgctgg cccccggcga gcgacagtgg
     1621 cggcggctca cgctggctgc cgaccgcgcc ggcgtcaccc agcgtccctc ggagacgatc
     1681 tacgagtacg ccggctggct ggaggagcag atccccgctc gacgcggcga gatccggacg
     1741 atcgcccgcg gcaaggtctg gcagtcgtac tctggccacg cgctgacgac cgatgcgctg
     1801 gcgcgcatcg aggcggcctg gaagcggctc gagctgccgc tgatgtggct caccatccgc
     1861 cgctggttcc gcgccctgct gcccgggcgc tgacgatagt ccccggccct agagggcgag
     1921 cggctcgccc tcggactcgt cgacgtgcag tggcgcgcgc ccgagcaggt gcgccgtgtc
     1981 gttccagcgc tccacgcgcc aggcagagcc cgagccggtt gcgggtgcgg gcgccgtggc
     2041 ggtcaggacc gacagcgagg cgttgtccac gtccatggcc catgaccgaa tcgcgccgag
     2101 tcccaacagg tgccgcgccg cgaggcgaat gatgcccccg tgcgagacca cgcaggtcac
     2161 gtcccccggc gcggcggcca gctcgtgcag cagctcggcg acgcgggcca gcacggcttc
     2221 gatcgtttcg gccccgggtg gttgcgcctg gtcggagtgc tgccgccagg cggcgtagcg
     2281 ctccgcgtcc tcgaccgcca gctcggcatg ggtgcggccc tcccattccc cctggccgat
     2341 ctccatcagt cgcgggtcgt gctcgacatt gggtgcatcg tcgccgagcc cgcccgcgat
     2401 caggtcggcc gtggtcgccg cgcgctgaag tggagaggtc acgatgcggt ccggccggtc
     2461 aacgccatcc gccagtcgct ccgccagcag gcccgcctcc ttgcggccgt cgtcggccag
     2521 cgggggatcc aggtggccct ggaagcggcc ctcgcggttc catgccgtga tgccgtgacg
     2581 gacgaggagc aggcgggcgg tcacctcgcc gcggcggcgg cccgatcgaa ggcctcccga
     2641 gcggcctcca ccgcagcagg cgggtcgacc tcccggccgt ggtcgcgcag tgtctcgccc
     2701 atgacccgta ccgcatcgct catctcatcg agctcgacca tgcccatgtg gccgaagcgc
     2761 aggatgcgcc caacccactt cccctggccg ccggccacca ccaggtcgcg cgatcgcatg
     2821 tcgcggttga acggcggcca gtccagccca tccggcagcc aggcggcggt gaccgtgggg
     2881 gaccgatgct ccggcggcgc caccagccgc aggcccaggg cttccaggcc ggaccctgcc
     2941 gcggccgcga gggccgcatg gcgcgcccac gtccgctcgc gcccctcggc gcgaagccgc
     3001 tggacgccga cgcggagccc gaacaggacg ctgacggccg gcgtccacgg agtctgcccc
     3061 ttctccgccc acttgcgcgc ctcagcgaag tcccagtaga agcgcggaat ggcggccgat
     3121 gcctcggccg cgcgtgcccg ctctccgacg gccgcaatgg cgattcccgg cgatcccatc
     3181 cacgccttct ggctggcgct gagcgccagg tccacgcccc aggcgtccat ctcgaacgga
     3241 atggcgccca gaccgctgat gccgtccacg atggccagtg gctcgcccgg agcggcacgc
     3301 acggccgcaa ccagctcgcg cagcgggttg gccacgccgg tggacgtctc gttgtgcgtc
     3361 agcagcacgg cgcggaacgg tggtcgcgag gccaggtgct gctccaggac ggtcggatcg
     3421 gcggcagtgc cccactcgac ttccaggcgc tctacgtcag tgccgaaggc ggtggcgatg
     3481 gccgcgaacc gatcgccgaa ggagccgatc gagacggcga gcacgcggtc gcccggcgag
     3541 agcgtgttga ccaccgctgc ctcgagcgac ccggtgcccg acccggtgag aaggagaaca
     3601 tcgccgcgcg tgccgatgag ctgaccgatg ccggcggtga tctcgcgctg catctccccg
     3661 aactccgtgc cgcggtggtc gaccatcggc tgcgcctggg cgtggcgaac ctcgcgcggc
     3721 aggggaatgg gtcccggagt tcggagattc tggtccatga tcggcatcat agtcgtggga
     3781 cggcgcggcc ccggcccgat tacgggttct cgccacgtcc cgaacgccga cggaacgcca
     3841 cccacgcggc gatgcccatc gcgaccacgg acacgccgag gatcgcgacg gtcccaggcg
     3901 gagcctccgc cggagcaatc gaggccccca cgccgcgtat ctccaccgtc gtggctgcct
     3961 gcacgagact gtcgagctcg tcactcgtcg tgacgcggaa gttcggtgcg cacggagcga
     4021 cggtcgcatt cacgtcctcg atcgcccccg cgtgctcgag ccccaggaac cagtgctctc
     4081 ccaccctcca ggagtcgggc ggtggtggcc cgtccgagcg tgccgcgagc ctcatgcgct
     4141 gcgggacgtc gcctccgaat gcgtgggtcg gcgtcacgac gagcgtgatg ggctcgagct
     4201 cctcgcgctc gaccgattcg acgcggccga cgatatacgc gtctgcccag agtgggtgct
     4261 cgccggcgac c
//
LOCUS       DATACA010000087         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2181782, whole genome shotgun sequence.
ACCESSION   DATACA010000087 DATACA010000000
VERSION     DATACA010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181782"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="xseA"
                     /locus_tag="VHK28_01940"
     CDS             <1..1006
                     /gene="xseA"
                     /locus_tag="VHK28_01940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX2141023.1"
                     /translation="HGMQVICHGRLRAYEPQGVYQLYVMSMTPAGAGDLHQRYEALRA
                     KLAGEGLFEADRKRPLPRWPRRIGVATSPVGAVWRDITNVMRRRYPLAELVLSPTVVQ
                     GAAATGAIIRALQRLYARGDLDLVILARGGGSLEDLWPFNEERVVRAVAASPVPVIVG
                     VGHESDVTLCDFVADVRAPTPSAAAEQAVPDATQFPTILARLRDRAGTAAQAALARRQ
                     RFVAEEGRALGRLAPDVAAARQRTADLLDRAYRATLTRTEREAATLMRLRDGLRTLSP
                     DATMERGYAVARLADGTIVRDPDQAPPGSALAVTVARGRLATRVEQAERRGPAEEELA
                     "
     gene            1003..1248
                     /gene="xseB"
                     /locus_tag="VHK28_01945"
     CDS             1003..1248
                     /gene="xseB"
                     /locus_tag="VHK28_01945"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009806546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX2141024.1"
                     /translation="MTEQAETTQLDARPFDELVGELQRIVQSLEAGNLPLEESIALYR
                     RGLELHAACEARLRDAELKITQLGRGADAEGDAEGDG"
     gene            1241..>1659
                     /locus_tag="VHK28_01950"
     CDS             1241..>1659
                     /locus_tag="VHK28_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141025.1"
                     /translation="MASPVAATVIRGPLPSDAAVLAALATQLGYPTDPTEMSERLAAV
                     GANQLAAVFVAARPQDRPLGWIHVELKRSLASSGNALVAGLVVDEAARSAGIGRRLLD
                     RAEAWAREHGCQTMQVASRRTRERAHRFYLREGYTVL"
BASE COUNT          224 a          593 c          645 g          197 t
ORIGIN      
        1 gcacggcatg caggtcatct gccacggacg gctgcgggcc tacgagccgc agggcgtcta
       61 ccagctgtac gtgatgagca tgacgccggc gggcgccggc gacctgcacc agcgctacga
      121 ggcactgcgg gcgaagctgg caggcgaggg gctgttcgag gcggaccgca agcggccgct
      181 gccgcgctgg ccgcgacgaa tcggggtggc cacctcgccg gtcggcgccg tatggcgcga
      241 catcaccaac gtcatgcgcc gccggtaccc gctggcagag ctggtgctgt cgcccaccgt
      301 cgtgcaggga gcggccgcca ccggggccat catccgcgcc ctgcagcggc tctacgcccg
      361 cggtgacctg gatctcgtca tcctggcccg tggcggcgga tcgctcgagg acctgtggcc
      421 cttcaacgag gagcgggtgg tgcgcgcggt ggccgcttcg ccggtgcctg tcatcgtggg
      481 cgtcggccac gagagcgacg tgacgctgtg cgacttcgtg gcggacgtcc gcgctcccac
      541 gcccagcgcg gccgccgagc aggcagtgcc ggacgccacg cagttcccga ccatcctggc
      601 ccgcctgcgc gaccgggcag ggaccgcggc acaggcggcg ctggcccggc ggcagcggtt
      661 cgtggcggag gaaggtcgtg ccctggggcg cctggcgccg gacgtggcgg ccgctcggca
      721 gcggaccgcc gacctgctgg atcgcgccta ccgcgccacc ctgacccgca ccgagcggga
      781 ggcggcgacc ctgatgcggc tgcgggacgg gctccgcacg ctctcgccgg acgccaccat
      841 ggaacgaggc tacgcggtgg ccaggctggc ggacgggacc atcgtgcgcg atccggacca
      901 ggcgccgccc ggatcggcgc tggccgtgac cgtggcccgc ggccgtctgg cgacccgcgt
      961 cgagcaggca gagcgccgcg gcccggcaga ggaggagctg gcgtgaccga gcaggccgaa
     1021 acgacgcagc tggacgcgcg ccccttcgac gagctggtcg gcgagctgca gcggatcgtg
     1081 cagtcgctgg aggcgggcaa cctgccgctg gaggagagca tcgcgctcta ccggcgcggg
     1141 ctcgagctgc acgctgcctg cgaggcacgc ctgcgggacg ccgagctcaa gatcacccag
     1201 ctcggccgcg gggccgatgc agagggcgac gccgagggcg atggctagcc cggtcgccgc
     1261 aacggtcatt cgcgggccgc tgccatccga cgcggccgtc cttgcggcgc tggccaccca
     1321 gctgggctac ccgaccgacc caaccgagat gagcgagcgc ctggcggcgg ttggcgccaa
     1381 ccagctggcg gccgtcttcg tggccgcccg cccgcaggac cggccgctgg gatggatcca
     1441 cgtcgagctc aagcgcagcc tggcgtcatc gggcaacgct ctggtggcgg ggctggtcgt
     1501 ggacgaggcc gcccgcagcg ccggcatcgg gcggcggctc ctggacaggg cggaggcctg
     1561 ggcgcgggag cacggctgcc agaccatgca ggtcgcctcg cggcgcacgc gggagcgggc
     1621 gcatcggttc tacctgcgag aggggtacac cgtgctcaa
//
LOCUS       DATACA010000088         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_414550, whole genome shotgun sequence.
ACCESSION   DATACA010000088 DATACA010000000
VERSION     DATACA010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414550"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VHK28_01955"
     CDS             <1..876
                     /locus_tag="VHK28_01955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2141026.1"
                     /translation="ASLVNGRLLPSLGPQSVGFISVILVASALFVTWSPRWHTAWLLT
                     ACFMAAGMVTLSLAAGTPVDTAASELMAVGVAGLVSLVGHPLAHLRTRRLLEQQFELR
                     QLSRVALRQERQLARLNGELVLTARMDVVTGIGNRRALDEALASLTGQRLAAVLVDVD
                     HFKAFNDRNGHLAGDSALGRIGEILREVVRKGDLVFRYGGEEFLILIPGGDREGAGRL
                     AERLRQGVEDDVAFQQWGLTISAGVAVADRFSASSPLPLLRRADSALYQAKRTGRNRV
                     VVDDAALVAPGPSPG"
     gene            905..1777
                     /locus_tag="VHK28_01960"
     CDS             905..1777
                     /locus_tag="VHK28_01960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2141027.1"
                     /translation="MRFGLQIDSFSWPGRAPAIGPTLARITRTADDAGFDSIWVMDHF
                     FQIRGGGPPEAPMLEGMTALGFMAAHSERARLGLLVGGIHYRPPGLWIKANTTLDVLS
                     GGRAWFGIGAAWNEEESTALGFPMPPLRERFEWLEDTLQMAHAMWSGGSGSGERFEGR
                     RVTATRLLNSPQAISRPRVPIMIGGGGERKTLRLVAQYADACNVFGGPERIAAKYGVL
                     REHCEQLGRPYEEIERSVLKSVDLEREAPAQVVDRFGALAEAGAQHVIVTLRRVSDVG
                     RVERLGAEVLPQLR"
     gene            complement(1957..>2133)
                     /locus_tag="VHK28_01965"
     CDS             complement(1957..>2133)
                     /locus_tag="VHK28_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2141028.1"
                     /translation="DDRDESPGVKLTDAELLGMPLIFTISPRSLAAGGAEVTRRATGE
                     RSVRPLDELGELLG"
BASE COUNT          303 a          731 c          772 g          327 t
ORIGIN      
        1 gcctcgctgg tcaatggccg cctgctgccc agcctcgggc cgcagagcgt gggattcatc
       61 tcggtgatcc tggtggcgtc cgccctgttc gtgacctggt cgccgcgctg gcacacggcc
      121 tggctgctga cggcatgctt catggccgcc ggcatggtca cgctgtcgct ggccgccggc
      181 accccggtcg acaccgcagc atcggagctg atggcggtcg gcgtggccgg gctggtgagc
      241 ctcgtcggtc acccgctggc gcaccttcgc acgcgacgcc tcctggagca gcagttcgag
      301 ctccgacagc tgagtcgggt cgcgctgcgc caggagcggc agctggcccg gctcaacggt
      361 gagctggtgc tcaccgcgcg catggacgtg gtgaccggga tcggaaaccg ccgagcgctt
      421 gacgaggcgc tggcctctct taccgggcag cggctggcag ccgtgctcgt agacgtggac
      481 cacttcaagg ctttcaacga tcgcaacggg cacctcgccg gtgattccgc gctcggacgc
      541 atcggcgaga tcctgcgcga ggtggtgcgc aagggcgacc tggtattccg ctacggcggg
      601 gaggaattcc tgatcctgat ccccggcggt gaccgcgaag gcgcgggcag gctcgccgaa
      661 cgcctgcgcc agggggtgga ggacgacgtc gccttccagc agtgggggct caccatcagc
      721 gcgggcgtcg cggtggccga tcggttcagc gccagcagcc cgctcccgct gctgcgacgg
      781 gcggacagcg cgctctacca ggccaagcgc accggtcgca accgggtggt ggtcgatgac
      841 gcagcgctgg tcgctccagg cccatctccg ggctgaatgg gcgacggctg tagcattgcg
      901 gccgatgcga ttcggcctcc agatcgactc gttcagctgg ccggggcggg ccccggccat
      961 cggtcccaca ctggcgcgga tcacccgcac ggccgatgac gccgggttcg actccatctg
     1021 ggtcatggat catttctttc agatccgtgg tggtgggcct cctgaggcac cgatgctgga
     1081 ggggatgacc gccctgggct tcatggccgc ccattcggag cgcgcccgcc tgggactgct
     1141 ggtgggcggc atccactacc gcccgcccgg gctgtggatc aaggccaaca cgacgctcga
     1201 cgtcctctcc ggtggccgtg cctggttcgg gatcggggcg gcctggaacg aggaggagtc
     1261 gaccgccctg ggcttcccga tgccgccgct gcgcgaacgc ttcgagtggc tggaagacac
     1321 cctgcagatg gcgcacgcca tgtggagcgg cggctccggc agcggagagc gcttcgaggg
     1381 ccggcgggtg acggcgaccc ggttgctgaa ctcgccgcag gccatcagtc gcccgcgggt
     1441 cccgatcatg atcggcggcg gcggcgagcg gaagacgctg cgcctggtgg cccagtacgc
     1501 ggatgcctgc aacgtcttcg gcgggccgga gcgcatcgcc gccaagtacg gcgtgctgcg
     1561 cgagcactgc gagcaactcg gccggcccta tgaggagatc gagcgaagcg tgctcaagag
     1621 cgtcgatctc gagcgcgagg cgcccgcgca ggtcgtggac cgcttcggcg cccttgccga
     1681 ggccggggcg cagcacgtca tcgtcacgct gcgccgcgtg agcgatgttg gtcgggtgga
     1741 gcggctgggc gccgaggtct tgccgcagct gcgctgagat ccggccggcc tggtcaccta
     1801 ctggtggtaa aggcgacgaa ggggcggcgt catcgccgaa tcaccagcca gtgacgcctg
     1861 ccggcgacga atccggccca tccagggcct gccagacgct catcgtcgcg aataccaccg
     1921 gctgttggtc atcggaggcg ggacacctca gccgcgtcac cccagcagct cgcccagctc
     1981 atcgagcggc cgcaccgatc gctccccggt ggcgcgacgc gtcacctcgg cgccgccagc
     2041 ggccagcgac cgcggcgaga tggtgaagat cagcggcatg cccagcagct cggcatcggt
     2101 cagcttcacg cccggcgact cgtcacggtc gtc
//
LOCUS       DATACA010000089         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1057695, whole genome shotgun sequence.
ACCESSION   DATACA010000089 DATACA010000000
VERSION     DATACA010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057695"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VHK28_01970"
     CDS             complement(<1..689)
                     /locus_tag="VHK28_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX2141029.1"
                     /translation="MAGSDRLTSAPAGDRGLPETIGRPGTSAFHRLNPLTKATLATCT
                     AVGAVAIGGLAGPLILVGVATLLPAAAAGVLRPLARTAVLLALPIAVSAFLVNLFFFP
                     GGETVLVRIGPVVATAEGLGLALEILVRIMAISGAITLFYLTTRPADLVVDLERRGVS
                     RRVAFVAHASVQTVPAMVERAAQITDAQRARGLETEGSVWRRVRGLVPIVGPVILGSI
                     AEVEERTMALEA"
     gene            complement(668..>1619)
                     /locus_tag="VHK28_01975"
     CDS             complement(668..>1619)
                     /locus_tag="VHK28_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141030.1"
                     /translation="VAAFVAYAVTEYAQTGALSSISRQFDTRTIVLMPIAIAINIILG
                     ATVANALKIPIYLDSIGTILVGALAGPIPGALTGFLANILWTYVVPPPFQNGPAAAFA
                     VVAAVIGLMAGLAGRMGWLRPRLNRSTGQLAGGAVLTIAVILGMAWLAYLGWTSPAFG
                     GEVVFGVNEGSDVIFTILGWLVLFLVAATVVGLLAILFLRRDLTGAYVVVAGLATGIV
                     AALISAPISAGVFSGVTGGGTDFLVAALRQAGADIGAATLGQGLISDPIDKITTFFVV
                     YLILSAMARSTKARFPQGERLIEGDEGTLPGESGWRGATA"
BASE COUNT          258 a          581 c          586 g          194 t
ORIGIN      
        1 gcctcgaggg ccatcgtccg ctcctcgacc tcggcgatgg agcccaggat gaccggtccc
       61 acgatcggca ccagcccccg cacccggcgc cagacgctgc cctcggtttc caggcctcgc
      121 gcgcgctggg cgtcggtgat ctgcgcggcg cgctcgacca tggccggcac ggtctggacc
      181 gatgcgtggg ccacgaaggc cacccgccgg ctgaccccgc gccgctcgag gtccaccacc
      241 aggtcggccg gccgggtggt cagatagaag agggtgatgg cgccgctgat ggccatgatg
      301 cgcaccagga tctccagcgc caggccgagg ccctccgcgg tggccaccac cggaccgatg
      361 cgcaccagga ccgtctcccc gccggggaag aagaacaggt tgacgaggaa ggcagagacc
      421 gcgatcggca gcgccagcag gacggcggtg cgggccagcg ggcggagcac gccggctgcc
      481 gcggccggca gcagggtggc cacgccgacc aggatcagcg ggccggccag gcccccgatc
      541 gccaccgcgc cgaccgcggt gcaggtcgcc agcgtggcct tggtcagcgg attcagccga
      601 tggaacgcgg aggtaccggg gcgcccaatc gtctcgggca gtccccggtc cccggccggc
      661 gcggatgtca ggcggtcgct ccccgccacc cgctctcgcc gggcagcgtg ccctcgtcgc
      721 cctcgatgag ccgctcgccc tgcgggaaac gcgccttggt gctgcgggcc atggcgctca
      781 ggatcaggta gaccacgaag aaggtggtga tcttgtcgat cgggtcgctg atcaacccct
      841 gccccagggt ggcggcgccg atgtccgcgc ccgcctggcg cagcgcggcc accaggaagt
      901 cggtcccgcc gccggtcacc ccgctgaaga cgcccgcgct gatcggggcg ctgatcagtg
      961 ctgccacgat gccggtcgcc agcccggcga ccaccacgta ggcgccggtc aggtcgcgcc
     1021 gcaggaagag gatggccagc agcccgacca cggtggcggc caccaggaac aggacgagcc
     1081 agcccaggat ggtgaagatg acgtccgatc cctcgttcac gccgaacacc acctcgccac
     1141 cgaacgcagg cgacgtccag cccaggtacg ccagccaggc catgcccagg atgacggcga
     1201 tggtgagcac cgccccgccg gccagctggc cggtcgatcg gttcagccgc ggccgcagcc
     1261 aacccattcg cccggcgagg ccggccatca ggccgatgac cgccgccacc acggcgaacg
     1321 cggcggccgg gccgttctgg aagggcggcg gcacgacgta ggtccacagg atgttggcca
     1381 ggaagccggt cagcgcgccg ggtatcggtc ccgccagggc gccgaccaaa atggtgccga
     1441 tgctgtcgag gtagatcggg atcttcagcg cgttggccac ggtggcgccc aggatgatgt
     1501 tgatggcgat ggcgatcggc atgagcacga tggttcgggt gtcgaactgc cgggagatgg
     1561 agctgagggc gccggtctgc gcgtactcgg tgaccgcgta ggccacgaag gcggccacc
//
LOCUS       DATACA010000090         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2663577, whole genome shotgun sequence.
ACCESSION   DATACA010000090 DATACA010000000
VERSION     DATACA010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663577"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="VHK28_01980"
     CDS             <1..1707
                     /locus_tag="VHK28_01980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX2141031.1"
                     /translation="MRGWRPQFTSPEFSPASRESLHTARVVPVYRLSSGLTQRRVREL
                     LARVLERALPAVEDPLLPNERVGLLGLVDALRDAHFPEEAEDVPAALDRLAFDELLAL
                     QLTLSQARVARQALRAPRVEIDPAELERLRAALPFELTDDQRRAVGEVVEDLASDQPM
                     RRLLQGDVGSGKTAVAAVALAGVVRAGWQGALMAPTEILARQHHAGLAPLLDALGVRS
                     EFLSGSLAPTRKREIHDAIAGGMADVVVGTHAVISEAVTFARLGMAVVDEQHRFGVAQ
                     RAALQAKGGGLEPHVLALTATPIPRTLALTFYGDLSISTLRELPPGRQPIRTEVRDRS
                     ALPKIEAFLASEAAEGRQSFVVVPLVNESEAMAAASAEADAERLRAALPELRIGLVHG
                     QQRADLRDTTMQAFAAGELDTLVATTVIEVGIDIPNASVMLIEDAERFGLAQLHQLRG
                     RVGRGPHRSFCILLSDATDELARRRLDVVRGSSDGFEIAEADLVLRGAGNLLGTRQSG
                     LPPLKVASLFEPRHVELAGRARVLADSLVATDAALEKRPELARLRAELVASEASDDGD
                     AA"
     gene            1715..2272
                     /gene="rsmD"
                     /locus_tag="VHK28_01985"
     CDS             1715..2272
                     /gene="rsmD"
                     /locus_tag="VHK28_01985"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX2141032.1"
                     /translation="MRVIAGSAGGIALTAPRGDATRPVTDRVKETLFGILGDRVLDAR
                     VVDLYAGSGALGVEALSRGAAHATFVEHDRRALVAIRENLERTRLAASASVHGLEVAR
                     YLASAGDQRFDLAFLDPPYEERDILAPLERLAPLLAPGATVVVKHFWRTDVPAPPGLA
                     VARRRRFGETVLTFLETDAVEEPPP"
     gene            2269..2757
                     /gene="coaD"
                     /locus_tag="VHK28_01990"
     CDS             2269..2757
                     /gene="coaD"
                     /locus_tag="VHK28_01990"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2141033.1"
                     /translation="MSRVAVFPGSFDPMTMAHLDVARRAASLFDRLVVGVLSNPKKSP
                     LFAAQQRVAMISEAVAEFGGNVEVEAFDGLTVEFAERHGAGFIVRGLRAVSDFEAELQ
                     MAHTNRKLQRRVDTVFLMTALEFGYLSSTLAKEVAQFGGDVSGMVPGPVAEALQARFA
                     KG"
     gene            3074..3520
                     /locus_tag="VHK28_01995"
     CDS             3074..3520
                     /locus_tag="VHK28_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2141034.1"
                     /translation="MLEQAAVLELIDQLRVAVPDEVRQAKRITEEAGRITERAREEGD
                     AIIARAQEQAAQMLEERELVRMAQQRAGEILDAANHEAAEVRRGADEYAAGVLIRLEG
                     ECIKALTSIKRGIDILDERHRVRPSTNDVPHDASGEPEATEAATRQ"
BASE COUNT          503 a         1290 c         1393 g          505 t
ORIGIN      
        1 atgcgcggct ggcgtccgca gttcacttcg ccggagttct ccccggccag ccgcgagtcg
       61 ctgcacaccg cgcgggtggt gccggtctac cggctgtcct ccggcctgac ccagcggcgc
      121 gtccgcgagc tgctggcccg cgtgctggag cgagccctgc cagccgtcga ggacccgctg
      181 ctgcccaatg agcgggtggg gctgctcggg ctggtggatg cgttgcgcga cgcccacttc
      241 ccggaggagg cggaggacgt gccggcggcc ctggatcgcc tcgcgttcga tgagctgctg
      301 gcgctgcagc tgacgctgtc ccaggcacgg gtggcgcggc aggcgctgcg cgcgccgcgg
      361 gtcgagatcg acccggccga gctggagcgg ctcagggcag cgctgccctt cgagctgacc
      421 gacgaccagc ggcgcgccgt gggggaggtg gtcgaggacc tggcgtccga ccaacccatg
      481 cgccggctgc tgcagggcga cgtgggcagc ggcaagacgg cggttgccgc cgtggcactg
      541 gcgggcgtgg tgcgcgccgg ctggcagggg gccctgatgg cgccaaccga gatcctggcg
      601 cggcagcacc acgccgggct ggcgccgctg ctggacgcgc tcggcgtccg atccgagttc
      661 ctgtccggct ccctggcccc gacgcgcaag cgcgagattc acgacgcgat cgccggcggc
      721 atggccgacg tggtggtcgg cacccacgcg gtgatcagcg aggcggtcac cttcgcgcgg
      781 ctgggcatgg ccgtggtcga cgagcagcat cgcttcggcg tggcgcagcg cgccgcgctg
      841 caggcaaagg gcggcgggct ggagccgcac gtgctggcgc tgaccgccac acccatcccg
      901 cgcacgctgg ccctcacctt ctacggcgac ctctcgattt ccaccctgcg agagctgcct
      961 ccaggccggc agccgatccg caccgaggtc cgggatcgct ccgcgctgcc gaagatcgag
     1021 gccttcctgg cgtccgaggc ggccgagggg cgccagtcct tcgtggtcgt cccgctggtc
     1081 aacgagagcg aggcgatggc ggccgcgtcc gccgaggcag atgcggagcg gctgcgcgcc
     1141 gcattgcccg agctgcggat cgggctggtg cacggacagc agcgcgccga cctgcgcgac
     1201 acgaccatgc aggccttcgc ggccggcgag ctggacacgc tggtggccac gaccgtgatc
     1261 gaggtcggca tcgacatccc gaacgccagc gtgatgctca tcgaggatgc ggagcgcttc
     1321 ggcctggcgc agctgcacca gctgcgcggc cgggtcgggc gcgggccgca tcggtccttc
     1381 tgcatcctcc tctcggatgc gaccgatgag ctcgcgcgcc ggcgcctgga cgtggtgcgc
     1441 ggctcgtcgg acgggttcga gatcgcggag gccgacctgg tcctgcgcgg tgccggcaac
     1501 ctgcttggca cccggcagtc cgggctgccg ccgctgaagg ttgcgtccct gttcgagcca
     1561 cggcacgtgg agctggccgg acgggcgcgc gtcctggccg actcgctggt ggccaccgac
     1621 gccgcgctcg agaagcgtcc cgagctggcc cggctgcgcg ccgagctggt ggcaagcgag
     1681 gcctcggacg acggagacgc ggcgtgaggc gccggtgcgc gtgattgccg gatcggcggg
     1741 tggcatcgcg ttgacggcgc cgcgcggcga cgccacccgc ccggtcaccg accgcgtcaa
     1801 ggagacgctg ttcggcatcc tcggtgaccg ggtgctggac gccagggtgg tggacctgta
     1861 cgcgggcagc ggggcgctgg gagtcgaggc gctctcccgg ggagcggccc acgccacctt
     1921 cgtggagcat gaccgccggg cgctggtcgc catccgcgag aacctggagc ggaccaggct
     1981 ggcggcctcc gcatcggtcc atggcctgga ggtggcgcgg tacctggcat ctgcgggcga
     2041 tcagcgcttc gacctggcct tcctggatcc gccctacgag gagcgtgata tcctcgcgcc
     2101 gctcgagcgg ctggcgccgc tgctggcccc cggcgcgacg gtggtggtca agcacttctg
     2161 gcggaccgat gtgccggctc cgcccgggct ggccgtcgcg cgccggcggc ggttcggcga
     2221 gacggtgctg accttcctgg agaccgatgc agtggaggag cctcccccgt gagccgcgtc
     2281 gccgtctttc ccgggtcgtt cgaccccatg acaatggccc acctcgacgt ggcgcgccgg
     2341 gcagccagcc tcttcgaccg cctggtggtg ggcgtgctca gcaacccgaa gaagtcgccg
     2401 ctgttcgccg cgcagcagcg ggtggccatg atcagcgagg cggtggccga gttcggcggg
     2461 aacgtcgagg tggaggcttt cgacgggctg accgtcgagt tcgccgagcg gcacggcgcg
     2521 ggcttcatcg tccgcggcct gcgcgccgtg tccgacttcg aggcggagct gcagatggcc
     2581 cacaccaacc gcaagctgca gcgccgggtg gacaccgtct tcctgatgac cgccctggag
     2641 ttcggctacc tgtcgagcac gctggccaag gaggtcgccc agttcggcgg cgacgtgtcc
     2701 ggcatggtgc cgggaccggt ggcggaggca ctgcaggcgc gcttcgcgaa gggatagccg
     2761 gggtcagtgc ccgtctgtgc cgcacaggcg cacaggggca caaccggggt ctgacgcccg
     2821 cacggtggtc cgccggcacc accggctcgc tgtcatggcc cttcgtgcac cacagccgca
     2881 cggccgcacg gccgcacagc cccagcggat gccctggctg cgaccgccgc gccggcaccg
     2941 cagaggttga acggggcggg tctataatcg cgccacctga cggagggcac acgccacgga
     3001 catcatcttc ctcgtcgagc ggctggagag cctcgttgcc gcgggcaaga agctgcccct
     3061 cacgaacaac atcgtgctgg agcaggcagc cgtgctggag ctgatcgacc agctccgcgt
     3121 cgctgtgccc gacgaggtgc gccaggcgaa gcgcatcacc gaggaggcgg gacgcatcac
     3181 ggagcgggcg cgcgaggagg gtgacgccat catcgcccgt gcccaggagc aggccgcgca
     3241 gatgctcgag gagcgggagc tggtgcgcat ggcgcagcag cgcgcgggcg agatactgga
     3301 cgccgccaac cacgaggcgg ccgaggtgcg gcgcggcgct gacgagtacg ctgccggcgt
     3361 cctgatccgg ctggaggggg aatgcatcaa ggccctgacc agcatcaagc gcgggatcga
     3421 catcctcgac gagcgccatc gcgtgcgtcc gagcaccaac gacgttccgc acgacgccag
     3481 cggcgagccg gaggcgaccg aggccgctac ccggcagtga ccgtcttcaa cgtggcgggc
     3541 ctgctccggg agccacccgg ggcagtacga ggacatcgcc tgcgcgaccg atacgtctcc
     3601 ctggggcctg acgtggagct ggccggaccg atcaacggca ccatccgcat gcagcgcacc
     3661 aaccgcggca tcctggtgcg cggcgacctg g
//
LOCUS       DATACA010000091         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_575455, whole genome shotgun sequence.
ACCESSION   DATACA010000091 DATACA010000000
VERSION     DATACA010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575455"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..666)
                     /locus_tag="VHK28_02000"
     CDS             complement(169..666)
                     /locus_tag="VHK28_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141035.1"
                     /translation="MDWVTFGAQWLHVIFGITWFGYSLAMAIFIIPALNRLPIPVQRQ
                     FGTALGARTEPIIDVLAPTIIVLGVIRGTFLGPIDSVEEVFTTAYGITWLVALIATTA
                     VYLWSRFVIFPAVARMNAAPLTAEGGPTPELDAAIATAKGVVILELIGFLVIFTCMIL
                     MRFGL"
     gene            929..1879
                     /locus_tag="VHK28_02005"
     CDS             929..1879
                     /locus_tag="VHK28_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141036.1"
                     /translation="MKPIAILYEHPEWFKPLFAELDRLGAPYVKLHAGEHIFDPDERE
                     SPYSLVVNRMSPSAWMRGQREAIFHSLRYLEHLEAIGAPVFNGATAFRLELSKAAQYT
                     LMSRLGIRHPATRVLSRPDQVPAACEGLHFPVLVKPNIGGSGAGIQSFATPQELEAAV
                     TAGDISFGIDHVGLLQEHLPAEGDAIVRIELLGGRFLYAIRLLLTPGTFNLCPADYCD
                     LPGMADGVSGRGLPIEGFDPPPELVDQARRLVAAGGLDVGGVEYLVNARDREAYFYDV
                     NALSNFVADAPNVIGFNPYVNLAELIVDRAGVKASGATAA"
BASE COUNT          316 a          628 c          648 g          308 t
ORIGIN      
        1 cggtcccgcg tgagaggtca tcgccggcgt aatcgcgctc gggcggcacc agctcgcagc
       61 cgaagagacc gatgtagaac tcggccagcg cccgccagtc ggtcgcgatg atgttggtgt
      121 ggccgaagcg ggcacccagg atcggtggtc ggtctgtcat ggccgcactt agagcccgaa
      181 gcgcatgagg atcatgcagg tgaagatcac gaggaagccg atgagctcca ggatcacgac
      241 gcccttggcc gtggcgattg ccgcgtccag ctcgggcgtc gggccgccct cggccgtcag
      301 cggtgctgcg ttcatgcggg ccacggccgg gaagatgacg aagcggctcc acaaatagac
      361 cgcggtggtc gcgatcagcg ccaccagcca ggtgatgccg taggcggtgg tgaagacctc
      421 ctcgacggag tcgatgggtc ccaggaacgt gccgcggatg acgcccagca cgatgatggt
      481 cggggcaagc acgtcgatga tcggttccgt gcgcgcaccg agcgccgtgc cgaactgccg
      541 ctgaacgggg atcggcaagc ggttgagggc cgggatgatg aagatggcca tcgccagcga
      601 gtagccgaac caggtgattc cgaagatgac gtgcagccac tgcgcgccga acgtgaccca
      661 gtccatggtc ccctcgtatc accccaccga acgcgtcgtg tcggatctgg cgggacggta
      721 gcgggtcgcg gactgtcgag caacaccgtt cgcgcagggg caccgggagg aggtcgagac
      781 cgcgtgggag gtcgcttgtg aagcacagcc gcacagatcc tgtgcagcac aaccggggtc
      841 ccacagcgcg cacccctacc accagcaccc gcgtcccata ggggctgagt tgtgccgtgg
      901 gtggtatcgg tctaggctcc gcctcccgat gaagccgatc gccatcctct acgagcaccc
      961 ggagtggttc aagcctctct tcgctgagct tgatcgcctt ggcgccccgt acgtgaagct
     1021 ccacgcgggc gagcacatct tcgatccgga cgagcgcgag tcgccctaca gcctggtcgt
     1081 gaaccggatg agcccgtcgg cctggatgcg gggccagcgg gaggcgatct tccattcgct
     1141 gcgctacctg gagcacctcg aggccatcgg tgcgccagtg ttcaacggcg cgaccgcgtt
     1201 ccggctcgag ctctcgaagg cggcccagta cacgctcatg tcgcgcctgg ggatccgcca
     1261 tccggccacg cgcgtcctca gccggcccga ccaggtgccg gccgcgtgcg agggcctcca
     1321 ctttccggta ttggtcaagc cgaacatcgg tggcagcggg gccggcatcc agtcgttcgc
     1381 cacgccccag gagctggaag cggcggtgac tgccggcgac atcagcttcg ggatcgacca
     1441 cgtggggctg ctccaggagc acctgccggc cgagggcgac gcgatcgtgc gcatcgagct
     1501 cttgggcggg cgcttcctgt atgccatccg cctgctgctg acaccaggca ccttcaacct
     1561 ctgcccggcc gattactgcg acctgcccgg catggccgac ggtgtctccg gtcgcgggct
     1621 ccccatcgag ggcttcgatc ctccgcccga gctggtggac caggctcgtc ggctggtggc
     1681 cgccggtggc ctggacgtgg gcggcgtgga gtacttggtc aacgcaaggg accgcgaagc
     1741 ctacttctac gacgtcaatg cgctgtccaa cttcgtggcc gatgcgccga acgtgatcgg
     1801 tttcaacccc tacgtcaacc tggccgagct gatcgtggac cgcgctggcg ttaaggcatc
     1861 gggcgcgacc gcggcctgac gcctaactgc tcgattccaa
//
LOCUS       DATACA010000092         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2101351, whole genome shotgun sequence.
ACCESSION   DATACA010000092 DATACA010000000
VERSION     DATACA010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101351"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(665..1315)
                     /locus_tag="VHK28_02010"
     CDS             complement(665..1315)
                     /locus_tag="VHK28_02010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2141037.1"
                     /translation="MKGRIYLALAGALALAGCAFGSGDASPAPCPTAAPTAEQAAGVI
                     GDAEQVEVVTNKGTFVIDLAPEAAPIAVANFVALVRCDFYDGISFHRVLAGFVAQAGD
                     PQTKDNRGDFEALGTGGPGYRFVIEPPAEDLNYDPYVVAMANAGAPDTNGSQFFINLA
                     NLDARLERSYTIIGQVSEGTEVVDTIGQVPTNGPRGVPLDPVIIERMTVQDAAEEA"
     gene            complement(1312..>1632)
                     /locus_tag="VHK28_02015"
     CDS             complement(1312..>1632)
                     /locus_tag="VHK28_02015"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX2141038.1"
                     /translation="ARIILDVRLHRGDIGFDDAVDFLVEHTGFERHGALAEVRRYTST
                     PTYQLSYLYGRHLFDQLRQRVERRLGSEFDLRRFHDTLLYGGTMPIAFADRLFETELQ
                     PVDR"
BASE COUNT          280 a          533 c          538 g          281 t
ORIGIN      
        1 ggcgaaactg tacgcgactg tgcgcacggc ggtttgtcgt tttggtgaca cggccgggtg
       61 ggtatccacc tggtggctga ttcggccaac tggcagccga attacccgtc ccatgactgc
      121 gggggccaag aacagggctc aacttggctg tcccagtcgc ccggcggctg cgcgcgccac
      181 tcagtggccg ttacatcgtg ggatgaatat tgcccgaatg cgccccgcga ccaaaggacc
      241 tgagcccctc tacagacgct caccgagcgg gctggcacgc gaggtgcatc acatgaaggc
      301 atcgaccacg ccagctgaag gaggaagcca tgtactgggg cggagggatc atcgggctca
      361 tcctgctgat cctactgatc ctgctgctga cggggaacct gtaagcagcc caaccatcaa
      421 ggacatcgag gcaagaagaa ggcccctccg aagaggggcc ttcattgctg ccggattcgc
      481 taaccgcggg ggcatctttc acgcgcggtt tcccgcacta ccctaaccca aacctaccca
      541 tgaccctgcg atcagtggtg aatcgctgtg cgaacagcga acacatcata ggctgccgga
      601 cccccagagg tcaacggtct gggactaaga ttcctttacg tttcagagcg cttttgagcg
      661 tgaatcaggc ctcttcagcc gcatcctgga ccgtcatccg ctcgatgatg accgggtcca
      721 gcggcacccc gcgcggcccg ttggtcggca cctggccgat ggtgtccacc acctcggtgc
      781 cctcgctcac ctggccgatg atcgtgtacg agcgctccag ccgagcgtcg aggttggcca
      841 ggttgatgaa gaactggctg ccgttggtat ccggcgcgcc ggcgttggcc atggccacca
      901 cgtacggatc gtagttcagg tcctcggccg gcggctcgat cacgaaccga taccccggtc
      961 cgccggtgcc caacgcctcg aagtcgccgc ggttgtcctt cgtctgcggg tctcctgcct
     1021 gggccacgaa tccggccagg acgcggtgga agctgatccc gtcgtagaag tcgcagcgca
     1081 ccagggccac gaagttggcg actgcaatgg gtgccgcctc gggcgccagg tcgatcacga
     1141 aggttccctt gttggtcacc acctcgacct gctcggcgtc gccgatgacg ccggcggcct
     1201 gctctgcagt cggggcggcc gtcgggcacg gggcggggga ggcgtcgccc gacccgaatg
     1261 cgcagccggc cagcgcaagg gccccggcca gggccaggta aatccgcccc ttcatcggtc
     1321 caccggctgg agctcggtct cgaagagccg gtccgcgaac gcgatcggca tggtgcctcc
     1381 gtacagcagg gtgtcgtgga atcgtcgcag gtcgaattca ctgcccagcc gccgctcgac
     1441 gcgctggcgc agctggtcga agaggtggcg cccgtagagg tagctcagct ggtaggtggg
     1501 cgtgctggtg tagcgcctga cctcggccag ggcgccgtgg cgctcgaagc cggtgtgctc
     1561 gaccaggaag tcgaccgcgt cgtcgaagcc gatatcgccg cggtgcagcc gcacgtccag
     1621 gatgatgcgc gc
//
LOCUS       DATACA010000093         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_334750, whole genome shotgun sequence.
ACCESSION   DATACA010000093 DATACA010000000
VERSION     DATACA010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334750"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VHK28_02020"
     CDS             <1..1128
                     /locus_tag="VHK28_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005627796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX2141039.1"
                     /translation="MSVEEWRTFAATAPWSVARSAARRMGLRVHWEPDRALSPEGYHQ
                     VRGGIGYAIARSLAVAPFADVLWMETKTADLDEAREFAEAIHAAYPDKLLAYNLSPSF
                     NWDTTGMSEEQMRSFPEELGRLGYVFNFITYGGHQVDGLAAEEFATALLEDGMLALAR
                     LQRKLRLLESPYRTPQTLVGGPRADAALLATSGLTATTKAMGEGSTQVQHLVQTEVPP
                     RVLAAWLGRWAEHNGLGYQPRVTLRPYTSGSEVLELAVTDEADERLANIVFSTIADRR
                     GRSILSVRDQNTFDESLRRRRLMTLMHLFLIHRYRAVSVHYLTPTEDNRRQCERMREI
                     GIFAAVNDEVGAIIVADVSAETVAELAGKESHDRERLIVKG"
     gene            complement(1130..1639)
                     /locus_tag="VHK28_02025"
     CDS             complement(1130..1639)
                     /locus_tag="VHK28_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX2141040.1"
                     /translation="MLPSILILGSLVVVLTVVAVTVGGWPLAWRGVRTGSTLLLSVLP
                     QLVLGFVLAGLVTVLLPREVLASVVGEGSGLLGLALATVAGLATPGGPFLQFPLVAAL
                     MAGGAAPGPVAAYLTAWSLLGWNRILVWELPLLGPGFTFTRVAVSLVIPILVGLVVPV
                     VMRLLNRPS"
     gene            complement(1651..>2248)
                     /locus_tag="VHK28_02030"
     CDS             complement(1651..>2248)
                     /locus_tag="VHK28_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141041.1"
                     /translation="GSVRDQFFAVIRRRDLRLILGAALVAAGGRGLDIVAPFMLLYLR
                     GPLGIDEATTAWLYALLLVGAVIGPLLAGWVSDRIGRKRTLVAYYLLSAVGILSFLAA
                     GANVWLLAPLLLPFGTAVFSESPVLQAYLADRAAGPMRDVAFSVYFTFAFGIGAFWAF
                     IIGSVVNTWGYPVAFSLMAASYVGAAVLLVAVRDRAPE"
BASE COUNT          412 a          781 c          789 g          266 t
ORIGIN      
        1 atgtcagtgg aggaatggcg cacctttgcg gccaccgccc cgtggtcagt ggcccgctca
       61 gccgcgaggc gcatggggct gcgggtgcat tgggagccgg atcgcgccct gtcgccggag
      121 ggctaccacc aggtccgcgg cggcatcggt tatgcgatcg cacgctcgct ggccgtggct
      181 cccttcgccg acgtgctgtg gatggagacc aagaccgccg acctggacga agcgcgcgag
      241 tttgccgagg cgatccacgc cgcgtacccg gacaagctgc tggcctacaa cctgtcaccg
      301 tcgttcaact gggacaccac cggcatgagc gaggagcaga tgcgctcctt cccggaggag
      361 ctgggcaggc tgggatacgt cttcaacttc atcacctacg gcggccacca ggtggatggc
      421 ctggcagccg aggagttcgc gaccgccctg ctcgaggacg ggatgctggc cctggcccgc
      481 ctgcagcgca agctcaggct gctggagtcg ccgtatcgca cgccgcagac cctcgtcggc
      541 ggaccgcggg ccgacgccgc gttgctggcg acctcgggcc tgacggccac caccaaggcc
      601 atgggcgagg ggtcaaccca ggtgcagcac ctggtgcaga ccgaagtgcc tccccgggtg
      661 ctggccgcgt ggctggggcg ctgggcggag cacaacggcc tcggctacca gccgcgcgtc
      721 acgctgcggc catacacttc cggctccgag gtgctggagc tggcagtcac cgacgaggcg
      781 gacgagcggc tggcgaacat cgtgttcagc accatcgccg atcggcgggg gcgaagcatc
      841 ctgtccgtgc gcgaccagaa caccttcgat gaatcgctgc gccgtcggcg cctgatgacg
      901 ctgatgcacc tcttcctgat ccatcggtac cgggccgtat cggtccatta cctgacgccc
      961 accgaggaca accggcggca gtgcgagcgg atgcgggaaa tcgggatctt cgcggcggtg
     1021 aacgacgagg tgggcgccat catcgttgcc gacgtttcgg ccgagacggt cgctgagctg
     1081 gccgggaagg aaagtcacga ccgagagcgc ctgatcgtaa agggctagct caggagggcc
     1141 ggttcagcag gcgcatgacc accggcacca ccaggccaac caggatgggt atcaccaggc
     1201 ttactgccac gcgggtgaac gtgaagcccg gccccagcag cggcagctcc cagaccagga
     1261 tccggttcca gcccagcaag gaccaggcgg tcaggtaggc ggccactggg ccgggcgccg
     1321 ctccgccggc catcagggcg gcgaccagtg ggaactgcag gaacggcccg cccggtgtgg
     1381 ccaacccggc caccgtggcc agcgccaacc ccagcagccc gctcccctcg ccgaccacgc
     1441 tcgccagcac ctcccgcggg aggagcacgg tgaccaggcc ggccagcacg aagcccagca
     1501 ccagctgcgg caggaccgaa aggagcagcg tcgacccggt gcgcacgccg cgccaggcca
     1561 gcggccaacc accgacggtt accgccacca cggtcagcac caccaccagg ctgcccagga
     1621 tcaggatcga cggcagcatg cgacgcagcc tcattccggg gcgcggtcgc ggacggcgac
     1681 cagcaggacg gcggcgccca cgtaggaggc cgccatgagg gagaacgcga ccggatagcc
     1741 ccaggtgttc accaccgaac cgatgatgaa ggcccagaag gcaccgatgc cgaaggcgaa
     1801 cgtgaagtac acgctgaagg ccacgtcgcg catgggcccg gccgcccggt cggccaggta
     1861 ggcctgcagc accggggact cgctgaagac cgcggtaccg aacgggagca gcagcggcgc
     1921 cagcagccag acgttggcac cggcggccag gaaggacagg atgccgaccg ccgacagcag
     1981 gtagtaggcg accagggttc gcttgcgacc gatgcggtca gacacccagc cggccagcag
     2041 cggcccgatc acggcgccca ccagcagcag ggcgtacagc caggcggtgg ttgcctcgtc
     2101 gatgccaagc gggccgcgca ggtagagcag catgaacggc gcgacgatgt ccaggccacg
     2161 gccgccggcc gccaccagtg ccgcgccgag gatcagccgg aggtctcggc ggcggatgac
     2221 ggcgaagaac tggtcgcgca ccgagccc
//
LOCUS       DATACA010000094         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_174484, whole genome shotgun sequence.
ACCESSION   DATACA010000094 DATACA010000000
VERSION     DATACA010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174484"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VHK28_02035"
     CDS             <1..1311
                     /locus_tag="VHK28_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2141042.1"
                     /translation="PETSRFLVWRAQTSAVVSSLFDASVYLAGNVADGFAAKVDAPAG
                     SLASSPRCGGLSVAPEPASLPNMLSVVGSGGPEDARLKANVNERTGTFVNGVGFPAPN
                     PSWSTEAALPDATPGAAPTPEPTAPPALSTPPPDGVSRLAGTDRYGTAAAASRATFGS
                     GVPVAYVASGASFPDALAAGSVAAKRAGPLLLVRPDGVPPDTATELARLRPEKIVVLG
                     GTRAVPATVATALARFAPVSRLDGVDRYATAAAASRATFGSGVPVVYVASGVSFPDAL
                     AAGSVAAKRTGPLLLVRPDAVPAATATELARLQPGKIVLLGGPRAVSGAVATALARLS
                     SGGVTRLAGADRYGTASAASAATFGSGVPVAYVASGSSFPDALAAGSIAAKRTGPLLL
                     VRPDGVPATTATELARLNPDEIVVLGGTGRIPDSVLRALGACID"
BASE COUNT          199 a          584 c          555 g          231 t
ORIGIN      
        1 ccggagacca gccgcttcct ggtgtggcgg gcccagacca gcgcggtggt ctccagcctg
       61 ttcgacgcca gcgtgtacct ggccggcaac gtggccgacg gcttcgccgc caaggtggat
      121 gccccggccg gcagcctggc cagcagcccg cgctgcggcg gcctgtcggt cgcgcccgag
      181 cccgcgtccc tgccgaacat gctcagtgtg gtcggatccg gcgggccgga ggatgcgcgc
      241 ctgaaggcca acgtgaacga acgaactggc accttcgtca acggcgtcgg cttcccggct
      301 ccgaacccgt cctggtccac ggaggcggcc ctgccggacg ccacccccgg cgccgcgccg
      361 acgccggagc cgacggcccc gcccgctcta tccacgccgc ctccggacgg cgtgagtcgc
      421 ctggcgggaa cagaccgcta cggcaccgcg gcagcggcgt cgcgagccac cttcggctct
      481 ggcgtgccag tcgcgtacgt ggctagcggc gcttcgttcc cggacgctct ggctgccggc
      541 tccgtcgcgg ccaagcgggc aggtccgctg ctgctggtcc gtcccgatgg cgtacctccg
      601 gacaccgcca ccgagttggc gaggctgcgt ccggagaaga tcgtcgtcct gggcggcacc
      661 cgtgccgtgc cagccaccgt ggcgacggcc ctcgcacgct tcgcgccggt cagccggctg
      721 gacggcgttg accgctacgc cactgcggcc gccgcctcgc gagccacgtt cggctcgggt
      781 gtaccggtgg tctacgtggc cagcggcgtt tccttcccgg acgcattggc cgcaggttcg
      841 gtggcggcca agcggaccgg tccgttgctg ctggttcgtc ccgatgccgt gccggctgcc
      901 acggccaccg agctcgcccg cctacagccg gggaaaatcg tgctcctggg cgggccgcgt
      961 gccgtatccg gtgccgtggc cacggccctg gcccgcctct cgtccggcgg cgtgactcgc
     1021 ctggccgggg cggatcgcta cggaaccgca tcggcagcat ccgctgccac cttcggctcc
     1081 ggggtgccgg tggcctacgt ggccagcggc tcgagcttcc cggacgcgct ggcagcggga
     1141 tcaattgcag ccaagcgcac aggcccgctg ctgctggtcc gcccggacgg ggttccggcc
     1201 acgaccgcga ccgagttggc gcgcctgaac ccggacgaga tcgtcgtcct gggcggcacc
     1261 gggcggatcc cggacagcgt gctgcgcgcg ttgggtgcct gtatcgactg atcggctcgt
     1321 ggccgtttcg gccaccggac tgcggtctcc gctgacccgc ctttcgctca cggcacgacg
     1381 cgtgcacgcc ttcggctgtg acacgaagag gaaccgcagg aggagatgat gcagtcgact
     1441 gatgccggtc gcacgcagac gaccgtgggc gaggaggcgc gagagcagga gcactcgcac
     1501 gagccgctca cccatgtcca cgatcactac cacgtgaccc accatcacgc gggtggtccg
     1561 ctgggcgag
//
LOCUS       DATACA010000095         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1219159, whole genome shotgun sequence.
ACCESSION   DATACA010000095 DATACA010000000
VERSION     DATACA010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219159"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VHK28_02040"
     CDS             complement(<1..478)
                     /locus_tag="VHK28_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141043.1"
                     /translation="MSERDELEARRRAERFERYLDALLDGGRPSPEDVADRDEAEMAR
                     LAAELSAASDPQAAPDPAFLEQVRARMRQADQGIRSVQTPLPVRPRPQAGAGGPATGS
                     VRISRRQLLAGGVVGAAGLAAGALGASVLRQPEDGPLLWDDGSDLVAGEGEWREVAS"
     gene            complement(471..1001)
                     /locus_tag="VHK28_02045"
     CDS             complement(471..1001)
                     /locus_tag="VHK28_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2141044.1"
                     /translation="MADPTHRPAEQRGRFEEIYDEHVVPIYRYIYARVGNRPDAEDLT
                     AQVFMRAVEQLDTTRDPGQIASWLYRVSQNVIADYWRAFYRLPLVGADQVAPGWEPRE
                     PVRERTSDDGAAIARVRQLLERLPERYARVLELRFLQRMSVAETAERMGISHGNAKIL
                     QYRALRKAALLGDGDE"
     gene            complement(1036..1530)
                     /locus_tag="VHK28_02050"
     CDS             complement(1036..1530)
                     /locus_tag="VHK28_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2141045.1"
                     /translation="MSHPVAEERPVEHGDALNLVRTIIAEAPGPEAAAQRAVELLHDR
                     FGHYDWLGIYWVEGDDLVLGPWIGPEATEHTRIPIGVGICGAAAASGQTEIVDDVDAD
                     PRYLACFASTRSEIVVPIFAEGRVVGEIDIDGSDPGAFTDTDARFLEEVAALLAPLRP
                     RHEA"
     gene            complement(1544..1921)
                     /locus_tag="VHK28_02055"
     CDS             complement(1544..1921)
                     /locus_tag="VHK28_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141046.1"
                     /translation="MDAGLPVPTPGTHRSRAARRAAARRADSAATAALNILGQKWVLR
                     IVRALGDRTQRFCELQDALGGANSATLSQRLKLLEDEGLVDRRAVSETPPWVEYSLTV
                     KGTDLHRAIAGIDRWADRWASQQ"
     gene            complement(1989..2447)
                     /locus_tag="VHK28_02060"
     CDS             complement(1989..2447)
                     /locus_tag="VHK28_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2141047.1"
                     /translation="MRHSHAPRRRRSPAVLAALISLLLVLAACASAATDAASAEPSEA
                     ASTEASASTAASEGAEPSDGAAQEVSINGTSFGADEITVAVGDTVTYTNHSSLPHTVT
                     EGTDGNTADGARFNEDLPAGESVEITFEEAGDYDVTCLFHSNMNMVVHVE"
     gene            2636..3403
                     /gene="ccmA"
                     /locus_tag="VHK28_02065"
     CDS             2636..3403
                     /gene="ccmA"
                     /locus_tag="VHK28_02065"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX2141048.1"
                     /translation="MPQTTIYSAPRTGPHPPPPAVATDQLARLFGRSVALAGVSLRVE
                     AGRSIALLGPNGAGKTTLLRILATSLRPSFGSAAVDGVDVAERPDLVRRQVAYLSHAT
                     GLYDDLTAEENLRFAARLRRVPDAPASVARVLAEVGLRPDAGRRVRGFSAGMRRRLAL
                     GRLLLATAPIVLLDEPHAALDAEGMALVDRLLAAWRGEGKTVLLASHQTARIMPLVDG
                     WARLDGGLLVEVGGMGVAGDSQVVMPVPAAAGAVANR"
     gene            3400..4092
                     /locus_tag="VHK28_02070"
     CDS             3400..4092
                     /locus_tag="VHK28_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX2141049.1"
                     /translation="MMASVRDELGLAWAIARKDALSELRGRQAAGSTLFFAALVLLLF
                     GFALGPDSRLLADAAPGLLWLAIVFGGLLTVARLHQLEADDGALDELVRYPVERGTIY
                     LGKAMAGGLAMVALGAVLLPVVAILYGLDLASALPALLATLLLGTIGFAAVGTFYAGV
                     TVRLRAREVMLPLLMLPVIAPLLLAAVSATAAALAGDPFGELAAWLQLLIGFDVVMLI
                     AGAAGYGYLLEE"
BASE COUNT          496 a         1500 c         1481 g          617 t
ORIGIN      
        1 gactggcaac ctcgcgccac tcgccctcgc cggcgaccag gtcgctgcca tcgtcccaaa
       61 ggagcggccc gtcctccggc tggcgcagga ccgacgcgcc gagggcgccg gccgccagcc
      121 cggccgcccc caccactcct ccggccagca gctgtcggcg gctgatccga acgctgcccg
      181 tggccggccc gccggcgccg gcctgcggac gcggccgtac cgggagcggt gtctggaccg
      241 aacggatgcc ctggtccgcc tggcgcatgc gcgcccgtac ctgctccagg aaggcggggt
      301 cgggcgcggc ctgcgggtcg gaggcggcag acagctcggc cgccaggcgc gccatctccg
      361 cctcgtcgcg gtctgccacg tcctccggtg atggccggcc gccatccagc agcgcgtcca
      421 ggtagcgctc gaagcgctcg gcgcgacgcc gggcctcgag ctcgtcccgc tcactcatcg
      481 ccgtcaccca ggagcgccgc cttgcgcagc gcgcggtact ggaggatctt ggcgttgccg
      541 tggctgatgc ccatgcgttc ggccgtctcg gccaccgaca tgcgctgcag gaagcgcagc
      601 tccaggacgc gggcgtagcg ctccgggagc cgctccagca gctggcggac gcgcgcgatc
      661 gccgcgccgt cgtccgaggt gcgctcgcgg accggttccc tgggctccca gccgggggcc
      721 acctggtccg cgccgaccag cggcaggcga tagaacgcgc gccagtagtc ggcgatcacg
      781 ttctggctga cccggtacag ccagctggcg atctgcccgg gatcgcgggt ggtgtccagc
      841 tgctcgacgg cgcgcatgaa gacctgcgcg gtgaggtcct cggcgtcggg ccggtttccc
      901 acgcgggcgt agatataccg atagatcggc acaacgtgct cgtcgtagat ctcctcgaat
      961 cggccgcgtt gctcggcggg acggtgcgtc gggtcggcca tcgctgcgga tacgcggcgg
     1021 gttacgccgg ccgcgtcagg cttcgtggcg aggtcgaagc ggagcgagga gggcggccac
     1081 ctcctcgaga aaacgggcat cggtgtcggt gaacgcgccc ggatccgagc cgtcgatgtc
     1141 gatctcgccc accacccggc cctcggcgaa gatcggcacc acgatttcgc tgcgggtgga
     1201 ggcaaagcag gccaggtagc gcgggtcggc gtccacgtcg tcgacgatct cggtctggcc
     1261 ggacgcggcc gccgcgccgc agatgccgac cccgattggg atgcgggtgt gctcggtggc
     1321 ctccgggccg atccacgggc cgagcaccag gtcgtctccc tcgacccagt agatgcccag
     1381 ccagtcgtaa tggccgaatc gatcgtggag cagctccacg gcgcgctgcg cagcggcctc
     1441 cggaccgggc gcctcggcga tgatggtgcg caccaggttc agcgcgtcgc cgtgctcgac
     1501 cggccgctcc tcggccaccg ggtggctcat cggcggcgag gcctcattgc tgcgaggccc
     1561 agcggtccgc ccagcggtcg atgccggcga tggcgcggtg caggtcagtg cccttgaccg
     1621 tcagcgagta ctccacccac ggaggcgtct cgctcacggc gcgccggtcg accagcccct
     1681 cgtcctccag cagcttcagc cgctgcgaca gcgtggccga gttggcgccg cccagcgcat
     1741 cctgcagctc gcagaagcgc tgcgtccggt cgcccagcgc gcgcacgatg cgcagcaccc
     1801 acttctggcc caggatgttc agcgccgcgg tggcggcgct atcggcgcgc cgcgccgctg
     1861 cccgccgcgc cgcccgcgag cggtgcgtcc cgggggtggg tacggggagc ccggcgtcca
     1921 tcaggcggcg gacgatagca ccggcccatg ggagggtcaa ccgaagggaa cgggctccgc
     1981 cgcggtcatc actcgacgtg gaccaccatg ttcatgttgg agtggaacag gcaggtgacg
     2041 tcgtagtcgc cggcctcctc gaaggtgatc tccacgctct cgccggccgg caggtcctcg
     2101 ttgaagcggg cgccgtcggc cgtattgcca tcggtcccct ccgtcacggt gtgcggcagg
     2161 gacgagtggt tggtgtaggt caccgtgtca ccgaccgcga ccgtgatctc atcggcgccg
     2221 aagctggtgc cgttgatcga aacctcctgc gcagcgccgt cggagggttc cgcgccctcg
     2281 cttgcggcgg ttgacgccga ggcctcagtg gacgccgctt cgctcggctc ggcgctggcc
     2341 gcatcggttg ccgccgaggc gcaggcggcc aagaccaaca gcaggctgat cagcgcagcc
     2401 aggacggccg gcgaccgccg ccggcgggga gcatgggagt gacgcatttg cggctacctc
     2461 gaaggtcgtg catgaaggtg gagtgatggg cgccaccgcg ccgccccgtc caggctgctc
     2521 gatggcgccc gatgcctcat acgcgtccgg tttcacacgc cgtccgccat ttgacgccca
     2581 tccgggcagg tcgcacactc aggcacaccc accgtcagcc ccggacgcgt cgagcatgcc
     2641 gcagaccacc atctactccg cgccgaggac cggcccgcac cctccacccc cggccgtcgc
     2701 caccgaccag ctggcgcggc tcttcgggcg gagcgtggcg ttggctggcg tctcgctgcg
     2761 cgtcgaggcc ggccgctcga tcgccctgct gggcccgaac ggggcgggta agaccacgct
     2821 gctgcgcatc ctggccacct cgctgcggcc gtcgttcggc agcgcggccg tcgacggcgt
     2881 tgacgtcgcc gaacgcccgg acctggtgag gcggcaggtc gcctacctgt cacacgccac
     2941 cggcctgtat gacgacctga ccgcggagga gaacctgcgg ttcgcggcgc gcctccgccg
     3001 cgttccggat gcgccagcct ccgtggcccg cgtgctggcc gaggtcggcc tgcgcccgga
     3061 cgctggccgg cgggtgcgcg ggttctcggc gggcatgcgg cggcggctgg cgctcggacg
     3121 cttgctcctg gccactgctc caatcgtgct cctggacgag ccacatgccg ccctggacgc
     3181 tgagggcatg gcgctggtgg atcgtctcct ggccgcctgg cgcggcgagg gcaagacggt
     3241 gctgctggcc tcgcaccaga cggcccgcat catgcccctg gtcgacgggt gggcccggct
     3301 ggatggcggc ctgctggtgg aggttggcgg catgggcgtc gccggcgact cgcaggtcgt
     3361 tatgccggtt ccggccgcgg ccggcgcggt ggcgaaccga tgatggcctc cgtccgcgac
     3421 gagctcggcc tggcctgggc gatcgcccgc aaggacgccc tctccgagct gcgcggccgg
     3481 caggccgccg gctcgacgct cttcttcgcc gccctggtcc tcctcctgtt cggcttcgcc
     3541 ctgggcccgg acagccggct cctggccgat gccgcgccgg gcctgctgtg gctggccatc
     3601 gtcttcggcg ggctgctgac cgtcgcgcgc ctgcaccaac tggaggccga cgacggcgcg
     3661 cttgacgagc tggtgcgcta cccggtggag cgggggacca tctacctggg caaggccatg
     3721 gccggtgggc tggccatggt cgcgctgggc gccgtgctgc tgccggtggt cgccatcctg
     3781 tacggcctgg acctggcctc ggcgctgccc gcgctgctcg cgaccctcct gctggggacc
     3841 atcggcttcg ccgccgtggg caccttctac gccggggtca ccgtgcgcct gcgggcacgg
     3901 gaggtcatgc tgccactgct catgctgcca gtcatcgccc cgctgctgct ggccgcagtg
     3961 agcgctacgg ccgccgcgct tgccggcgat ccgttcggcg agctggccgc ctggctgcag
     4021 ctgctgatcg gcttcgacgt cgtgatgctc atcgccgggg ccgccggcta cggctacctg
     4081 ctggaggagt gatg
//
LOCUS       DATACA010000096         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_656944, whole genome shotgun sequence.
ACCESSION   DATACA010000096 DATACA010000000
VERSION     DATACA010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656944"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VHK28_02075"
     CDS             complement(<1..221)
                     /locus_tag="VHK28_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 family protein"
                     /protein_id="HEX2141050.1"
                     /translation="MALVTWILRSEAVAALVAGIGLWLVNDGSLLWLIPALLVPDVSA
                     IGYALNPRVGAVTYNLVHNWTLALVLLGLA"
     gene            complement(230..904)
                     /locus_tag="VHK28_02080"
     CDS             complement(230..904)
                     /locus_tag="VHK28_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX2141051.1"
                     /translation="MSEIMKVDVWSDIACPWCYIGKRNLEQAIRAAGTDAGSPSVQVE
                     FHSFQLSPDTPVDFDGSETDYLAQHKGISREQAAAMQERVTAIAAAAGLDYHFERVRH
                     TNTVKAHQLLHYARTRGLQSEMAERLMRGYFIEGRHLGRDEELVALAGEIGLDAEDVR
                     RSLREEEFLAAVRQDEGLAAAYGVRGVPFFVIDRGYALQGAQPPEVFEEALRMVANER
                     AAPAAT"
     gene            complement(1010..1978)
                     /locus_tag="VHK28_02085"
                     /pseudo
     CDS             complement(1010..1978)
                     /locus_tag="VHK28_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
BASE COUNT          299 a          701 c          675 g          306 t
ORIGIN      
        1 gccagcccga gcaacaccaa cgccagcgtc cagttgtgca ccaggttgta ggtaaccgcg
       61 ccgacgcgag gattcaacgc atagccgatt gccgacacgt cggggaccag taacgccggg
      121 atcagccaca gcagcgaccc gtcgttgacc agccagagac cgatgccggc caccagggct
      181 gccaccgcct ccgagcgcag gatccatgta accaaggcca tcgagccggc tacgtcgccg
      241 ccggagcggc ccgctcgttg gcgaccatgc gcagcgcttc ctcgaagacc tccggtggtt
      301 gggccccttg taacgcgtac ccgcggtcga tcacgaagaa cggcacgccg cggactccgt
      361 acgctgcggc cagcccctcg tcctgccgca cggccgccag gaactcctcc tcgcgcaggg
      421 accgccgcac gtcctctgcg tccagcccta tctcaccggc cagggcgacc agctcctcgt
      481 cgcggccgag gtggcgcccc tcgatgaagt agccgcgcat cagccgctcc gccatctccg
      541 actgcagtcc gcgggtccgc gcgtagtgca gcagctggtg ggccttgacc gtgttggtgt
      601 ggcgcacgcg ctcgaagtgg tagtcgagcc cggcggccgc ggcgatggcc gtcacgcgtt
      661 cctgcatggc agccgcctgt tcgcggctga tgcccttgtg ctgcgcgagg taatccgtct
      721 cgctgccatc gaagtcgacg ggcgtgtccg gcgacagctg gaagctgtga aactccacct
      781 ggaccgatgg gctgcccgca tcggtgccgg cggctcgaat ggcctgctcg aggttgcgct
      841 tgccgatgta gcaccacgga caggcgatgt cggaccagac gtcgaccttc atgatttcgc
      901 tcatgctgcc aggctcatcg gtcatgcgca gcatgaggcg cccagcgagc aggccgccgg
      961 gaggcatggg tgccatgcgc cagactggct gaggatcgat cacgggggat cacgccagat
     1021 cagggttggg cgattccgag atggtggtcg gggcgccgtg gacggcaggg atggagccca
     1081 gccggccggc ctggaagtct tcgaaggcct gcatcagctc cgcgcgggtg ttcatcacga
     1141 acggcccggc ccaggccacc ggctcgcgga tgggccggcc acccaggacc aggacgtcaa
     1201 gggtcgggct cctgctctcc tggcgagcgg cggccgccac gcggaggacc gtgcctcctc
     1261 cgagcacagc cagctgcccg gcctggacct ggactcgctc ggccccgacc gatccgtggc
     1321 ctgcgagcac gtagaccagc gcgttgtagt cggcacgcca tggcacgacc agctcagcct
     1381 ccgggctgag cgtggcgtgc aggagggtca tgggcgtgta ggtcgtgccc ggtccggcat
     1441 gaccggccac ctcgccggcg atcacgcgca ccagggctcc cccatccgcg gaggccagca
     1501 gggcgacctc gccgcctcgg atgtcctggt agcggggcgg cgaccacttc tgcgctcgcg
     1561 gcaggttgac ccacagctgg atgccgtgga acagcccgcc gctgatcacc agcgcctccg
     1621 gtggcttctc gatgtggagg atcccggcgc cagccgtcat ccactgggtg tcgccattgg
     1681 tgatcactcc cccacccccg ttcgagtcgc tgtgctcgaa cgtcccgtcg atcatgtagg
     1741 tcaccgtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttcggctcg ccgggcgcgt
     1801 actcgacctc ccccatctgg tccatgtgga tgaacgggtc caggtccccc aggtcgacgc
     1861 cgtggaaggc gcggcgcacc gggaatccct cgccctagaa cccgcgcggg gcctgtgtca
     1921 cgctcttgat gggtcgggcg acgctcgtga tcggatcggg ctcgggcagg cggggcagga
     1981 c
//
LOCUS       DATACA010000097         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1139467, whole genome shotgun sequence.
ACCESSION   DATACA010000097 DATACA010000000
VERSION     DATACA010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139467"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1817)
                     /locus_tag="VHK28_02090"
     CDS             complement(<1..1817)
                     /locus_tag="VHK28_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX2141052.1"
                     /translation="MAAVALPATLAGLCLGILGVDQGWLPPTGVPATAGATLLLAGRM
                     LRSPSVLAVGVLLLGVALGAWRGPAAALPVGSSSLTAHTGATSWQISGTVIDDPRPRG
                     ERQQVVLDRVTLEGPPAAPAAAEGRLLLWIPRAVEVRSGDRVRFRAPLEEPRDFDGFA
                     YREYLARQGIGAISRAFAMTVEHGAEGVPGAPLVALRQALLTDLNAVVPEPEAALAAG
                     ILLGVRAGIAPEVSEAFATAGLTHVVAISGWNIAIVAALAAAATRPLTRLQGGRWLAL
                     LAAFTTVGAYVLLTGASPSVVRAALMAAALVLARVGGSRAHALSALMVAALAMLVAAP
                     SVLWDVGFQLSALATAGLIWFGAPLERRLRRWPALVREPVALTMAAQLTTLPVILLNF
                     ERLSLVAPAANVVVVPLVPVVMLCSALAAVAGTLALSEIPIVGDALAWTTGGSAWLYL
                     RAMILAGQAAASVPFASVDLAAPVWLAGIWYPTLALGARRAGGRTDATADATPIDNMV
                     VRLARPRLVLGTTLALLVAVTVATRPDGRLHLVALDVGQGDAIVVVAPGGEVAMIDGG
                     PDPDVAMRRLGEVLPFWKRRIDVLVLTHPHEDHVAGLLPA"
     gene            complement(1845..>2502)
                     /locus_tag="VHK28_02095"
     CDS             complement(1845..>2502)
                     /locus_tag="VHK28_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX2141053.1"
                     /translation="PPSPPGRGGERMSLTVRDRIALGLAGCAAACVVAAGWLLVAAPT
                     SAGGLLGSSSLPPLPTMSTVAVGSPPPGGELVVDVQGGVVRPGVVVLPGGSRVADAIE
                     AAGGFADTADTIAAAGALNLAAPLTDGAQVYVPLKGVAGASHPPAGPAGPGGGNGLVN
                     LNSATPEELDALPGIGPVTVQKIVAARAERAFTTLDELVERDVMNRGQLDGIRDLVTI
                     "
BASE COUNT          376 a          956 c          910 g          260 t
ORIGIN      
        1 gccggcagca gcccggccac gtggtcctcg tgcgggtggg tcagcaccag cacgtcgatg
       61 cgtcgcttcc agaagggcag cacctcgcct agccggcgca tggccacgtc gggatccggg
      121 ccgccgtcga tcatggccac ctcgccgccg ggcgccacca ccacgatggc gtcaccctgc
      181 cccacgtcca gggcgaccag atgcagccgc ccgtcgggcc gggttgcgac ggtgacggcc
      241 accagcagcg ccagggtggt gcccagcacc aggcgtggac gtgccagccg caccaccatg
      301 ttgtcgatgg gagtggcatc cgcggtggca tcggtcctac cccccgcgcg tcgcgcgccc
      361 agcgccagcg tcgggtacca gataccggcc agccacaccg gtgccgccag gtcgaccgat
      421 gcgaacggca cggacgcggc tgcctgcccg gccaggatca tggcccgcaa gtagagccag
      481 gccgagccac cggtcgtcca ggccagcgca tcgcccacga tcgggatctc gctgagtgcg
      541 agggtgccgg ccactgcggc cagcgccgag cacagcatca ccaccggcac cagcgggact
      601 acgaccacgt tggccgctgg cgccaccagc gagaggcgct cgaagttgag caggatgacc
      661 ggtagcgtgg tcagctgagc ggccatggtc agcgccaccg gctcgcggac gagcgccggc
      721 cagcgccgaa gccgccgttc cagcggggcg ccgaaccaga tcagcccggc ggtggccagc
      781 gccgacagct ggaacccgac atcccacagg accgatggag cggcgaccag catggccagc
      841 gccgcgacca tcagcgccga cagggcgtgg gcccgggagc caccgacccg cgccagcacc
      901 agcgcggcgg ccatcaacgc ggcgcgcacg accgacggag aggcaccggt cagcagtacg
      961 taggcgccga ccgtggtgaa ggccgcgagc agcgccagcc agcggccgcc ctgcaggcgc
     1021 gtgagcggac gagtggccgc cgccgccagc gcggcgacga tggcgatgtt ccagccggag
     1081 atggcgacga cgtgggtcag gcctgccgtg gcgaaggcct cgctcacctc gggcgcgatg
     1141 cccgcccgta cgcccagcag gatcccggcg gccagcgccg cctcgggttc ggggaccacg
     1201 gcgttgaggt cggtcaacag cgcctgccgc agggcgacca gcggcgcacc cggcacgccc
     1261 tcggcgccat gctccaccgt catagcgaag gcccgcgaga tggctccgat cccctggcga
     1321 gccaggtact cgcggtaggc gaagccgtcg aagtcgcgcg gctcctcgag cggcgcgcgg
     1381 aagcgcacgc ggtcgccgga ccgcacctcg acggcccgcg gaatccagag cagcagccgc
     1441 ccctccgcgg cagcgggcgc ggccggcggg ccctcgagcg tcacccggtc gaggaccacc
     1501 tgctgccgct cgccccgcgg ccgagggtcg tcgatgaccg tcccgctgat ctgccagctg
     1561 gtggcgccgg tgtgtgccgt caggctggat gaccccaccg ggagggcggc ggccggaccg
     1621 cgccaggccc ccagggcaac cccgagcagg aggaccccga cggccagcac cgatggggac
     1681 cgcagcatcc gccccgcgag caggagcgtc gcgccggcgg tggccggcac gccggtaggc
     1741 ggcagccaac cctggtcgac gcccaggatg ccgaggcaca ggccggcgag cgtggccggc
     1801 agggcgacgg ccgccacgtc ggcagtcccc tgcctgcact gccgtcagat ggtgacgagg
     1861 tcgcggatgc cgtccagctg gccgcggttc atcacgtcgc gctccaccag ctcgtcgagg
     1921 gtggtgaacg ctcgctcagc ccgggcggcc acgatcttct ggaccgtgac cggaccgatc
     1981 cccggcagcg cgtccagctc ctccggcgtg gcgctgttca ggttcaccag gccgtttccg
     2041 ccgccgggtc cagccggtcc cgccggcggg tggctggcgc cggccacgcc cttgaggggc
     2101 acgtagacct gggcaccgtc cgtcagcggc gccgcgaggt tcagcgcgcc ggccgcggcg
     2161 atagtgtccg ccgtgtccgc gaatccgccg gcggcctcga tcgcgtcggc gacccgcgac
     2221 ccgccaggga gcaccaccac gccggggcgc acgactccgc cctggacgtc caccaccagc
     2281 tcgccgcccg gtggcggcga cccgacggcc accgtgctca tggttggcag cggtggaagg
     2341 gagctcgagc caaggagacc gccggctgac gttggcgcgg cgaccagcag ccagccagcc
     2401 gccaccacgc acgcggccgc gcagccggcc aggcccagcg cgattcgatc tcggacggtg
     2461 agggacatcc gttcgcctcc acggccggga ggcgacggag gg
//
LOCUS       DATACA010000098         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_255407, whole genome shotgun sequence.
ACCESSION   DATACA010000098 DATACA010000000
VERSION     DATACA010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255407"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VHK28_02100"
     CDS             <1..467
                     /locus_tag="VHK28_02100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141054.1"
                     /translation="AATGTLFALANVLAAISFPVAAWLAGRIGLIRTMVFSHIPANLF
                     LVGSALAPGIGLAVAFMLARAALSSMDVPARQSYTMAVVDPDERTATAGVTSLARSTG
                     QVMGPLLAGALLAPIGIGVPLIAAGVLKTAYDLSLYAVFRARPAPEEAITGA"
     gene            488..1756
                     /locus_tag="VHK28_02105"
     CDS             488..1756
                     /locus_tag="VHK28_02105"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2141055.1"
                     /translation="MNGKQVAGGSTGWVRHVLAIGSRPDDDPDLRVRKRTAVAAVYAA
                     IGAGIFYAALGIAADRPIVTALSVAQAAAQVAALAWFARTGRIDPMVVIVIVVGLLTI
                     ASGIVTFGGIQHSSGNVMFALLMPIVAVLVLGRRGAVPAFAAFVVLVLWAAITDPIWR
                     DLASPIPAELGVSLYAMNVIVAGGIALGTVVFIDGERVRAKRQAESLLLNVLPQSIVA
                     RLNAGERVIADHCPEVSVLFSDVVDFTPFAERQPPELVVAVLDDLFSAFDRLAQERGL
                     EKIKTIGDAYMVVAGVPQTRPDHAEVLVDMALAMHRVAGERAALDGHSIQLRSGIATG
                     PVVAGIIGRQKFSYDLWGDTVNLASRMESSGVPGCIQVSPATRERVAHLHAWQVREGI
                     EVKGKGPMRTYVLDPSQQVQPVSLAQPAQQ"
     gene            complement(2324..>2631)
                     /locus_tag="VHK28_02110"
     CDS             complement(2324..>2631)
                     /locus_tag="VHK28_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141056.1"
                     /translation="VAAMAVVGPAVLLVNPLTRTVLPGTFTFVVAGIVIASLLCGGRL
                     NLAVVGSAVAMVFASTAVPVQGGDLGWSMWLALVIGAWTIIPIVARHAAKQSSESRP"
BASE COUNT          384 a          897 c          911 g          439 t
ORIGIN      
        1 ccgcggccac cggcaccctg ttcgcgctgg cgaatgtgct ggccgccatc tccttcccgg
       61 tggccgcctg gctggcgggg aggatcgggc tgatccggac catggtcttc agccacatcc
      121 cggccaacct gttcctggtc ggttcggcgc tggcgccggg catcggcctg gcggtggcct
      181 tcatgctggc ccgcgccgcc ttatcgtcca tggatgttcc cgcccgccag tcgtacacca
      241 tggcggtggt cgaccccgac gagcgcaccg cgaccgcggg ggtcacctcg ctggcgcgca
      301 gcacggggca ggtgatgggg ccgctcctgg cgggcgcgct cctggcaccg atcgggatcg
      361 gcgtcccgct gatcgctgcc ggcgtgttga agaccgccta cgacctgtcg ctctatgccg
      421 tgttccgtgc gcggccggcc cccgaggagg ccatcaccgg ggcctgagcg gcgtaccctg
      481 cgcggcaatg aacgggaagc aggtcgccgg cggatcgacc ggttgggtcc gccacgtgct
      541 ggccatcggc agccgcccgg acgatgatcc cgacctgcgg gtccggaagc ggacggccgt
      601 tgcggccgtt tatgccgcga tcggggcggg catcttctac gccgcgttgg gcatcgccgc
      661 cgaccggccg attgtcaccg cgttaagcgt cgcccaggcc gccgcgcagg tggctgccct
      721 ggcctggttc gcgcgaaccg ggcggatcga tccgatggtg gtcatcgtca tcgtggtcgg
      781 ccttctgacc atcgcgtcgg ggatcgtcac cttcggtggc atccagcact ccagcgggaa
      841 cgtcatgttc gccctgctga tgcccatcgt ggcagtgctg gtgctgggcc gccgcggggc
      901 ggtgccggcc ttcgccgcct tcgtggtgct ggtgctgtgg gcggccatca ccgacccaat
      961 ctggcgcgac cttgcgtcac cgatccctgc cgagctgggc gtgtcgctgt atgccatgaa
     1021 cgtcatcgtg gcaggtggca tcgcgctggg gacggtcgtc ttcatcgacg gcgagcgcgt
     1081 ccgtgccaag cgccaggccg aatcactgct gctgaacgtc ctgccgcagt ccatcgtcgc
     1141 ccgcctgaac gccggggagc gggtgatcgc cgaccattgc ccggaggtat cggtcctgtt
     1201 cagcgacgtc gtggacttca cgcccttcgc cgagcgccag ccgccggaac tggtggtggc
     1261 cgtcctggac gacctgttct cggccttcga tcgcctggcg caggagcgtg gcctggagaa
     1321 gatcaagacc atcggcgacg cctacatggt cgtggccggc gtgccgcaga cgcgtcccga
     1381 ccacgccgag gtgctggtgg acatggccct cgccatgcac cgggtcgccg gggagcgggc
     1441 ggccctggac gggcatagca ttcagctgcg gtcggggatc gccaccggcc cggtggtggc
     1501 cggcatcatc ggtcgccaga agttcagcta cgacctgtgg ggcgacacgg tcaacctggc
     1561 gtcacgcatg gagtcgagcg gggtgccggg ctgcatccag gtcagcccgg ccacccggga
     1621 acgcgtcgcg cacctccatg cgtggcaggt ccgcgagggc atcgaggtca agggcaaggg
     1681 accgatgcgc acctacgtgc tcgacccgag tcagcaggtg cagccggtct cgttggcgca
     1741 gcccgcgcag cagtagctct tgccctcgcg ctccacgggg ttgctggcga tatccatctc
     1801 catcccgcag ttcgcacagt tctggtcggc catctggagc cacctcctgg gacagatgtg
     1861 cccgcatatt cggtaccacg tcggacgtgg cggtggctgc tgcccgatga agcgggcggt
     1921 ttttcactgg aacgagcggc tgcgtgtcaa atggccgttg tgtcgggcag cgccacgcat
     1981 gagccgacaa gaacttggcc gtccacgctc agggctgccc gatagatccg caggatcaca
     2041 cctgcccagg gcctcgcgtg aaatcctgcc cgatggctcg gctcgcgcag caccggtgag
     2101 gcaggatctc caggatggcg tccgcccgcg ctcggtccgg ggcgacatcg ttgggaaatg
     2161 ccgtaccggt gatgcgtaac ggagacgttc tcgccgtgga gggccttccg ggaccaacat
     2221 attcgccgtt aggccttacc cgcgcggcat ttccgcacga tcctgggacg ccgacggcac
     2281 ttcgtggaca gctcgccatg ccggcgccgc atgcgcctgt gagtcatgga cgggactcgc
     2341 tcgactgctt ggcggcatgg cgcgcaacga tcggaatgat cgtccatgcg ccaatcacca
     2401 gggcgagcca catcgaccag ccgaggtctc cgccctgaac gggcacggcc gtgctggcaa
     2461 acaccatggc caccgccgat ccaacgaccg caaggttgag ccgccctccg cagaggagcg
     2521 aggcgattac gatgccggca acgacgaagg taaaggtgcc tggcagaacc gtgcgcgtga
     2581 gcgggttcac cagcaggaca gccggaccaa ctaccgccat ggcggccacg a
//
LOCUS       DATACA010000099         4362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1059414, whole genome shotgun sequence.
ACCESSION   DATACA010000099 DATACA010000000
VERSION     DATACA010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059414"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VHK28_02115"
     CDS             complement(<1..716)
                     /locus_tag="VHK28_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141057.1"
                     /translation="MPDAGTVAIARIPLARARMRLLVAPLVLLVAGAVAAGGGVLLGG
                     FPGVGLVVAGIVVSVLAIYLALIPLTARLDVEVATLRLRWLGGERAFTLVRGAVTRVP
                     LVGEGAARLRPRFGALGWALGPARLRGEESIQLVRLAPTPSMILVPTDQGRFGIAAAS
                     EAQLLEALGAAARVQQRLDEVTARTRAFMPAVVPPPPPEIIEPVEHAAESGPRVMTGI
                     ERAILEERLAAQRAAALARA"
     gene            complement(719..2401)
                     /locus_tag="VHK28_02120"
     CDS             complement(719..2401)
                     /locus_tag="VHK28_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141058.1"
                     /translation="MSAPHDPDRFHWLQRLLEMVPGIISWAIIIGPIWLSFSYPWLVA
                     YFVLSFDFYWLCRALWFAGAVIVAFRRIRAVQAVSWRRRLDVLSDPVARREELLRRLS
                     ALSGRPSAEALGFAAGASARLGATPGGREQRVIERELADLERLDALPEPPPDWREYVH
                     LALIPTYTESLDKLRDTVRALVEADWPRERKICAIITRETDAEGRANVAALRDEFGHA
                     FAEFIHILDPLEPGIVIGKSSAMAWGGRYLYRLLVRERGMDARRILVTDLDADYRVHS
                     QYFTYLTWVHLTDANRETQLYQPIPYFHNNLWQAPFLQRLFAAVLTQLQMWRSVLPEK
                     LQSFGSYSTTLHLVHDVGYWATDAIPEDTRFYWKSFFRYGDRFRAVPLFIPIYGDAVR
                     ARTYWRSMASQYLQARRWAWGVTDIPYVVRNILVHPEIPLASRVWRLLNLFGEHVNWA
                     IAPFVVMFGATIPLVINPAFAETTLGQNLPFYASTMLSIGLLALAVLIWVEHRIVPPR
                     PAAWGRFKRLLSYVQWLGLPFVGIFFSNLPALDAQTRLLTGRYLEYRVTEKA"
     gene            complement(2459..3421)
                     /locus_tag="VHK28_02125"
     CDS             complement(2459..3421)
                     /locus_tag="VHK28_02125"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX2141059.1"
                     /translation="MLKQETRTADAPQEQTPELWAFFRGELVPLRDARVSVMTHAFNY
                     GTAVFEGIRAYWNADQSQLWALDLVPHYERLKASARFLMMEIPHTAEDLAARTIEMLR
                     RDGLREDVYIRPIVYKSSETIGVRLHNLDADVVIFGIPFGQYIDTEGGIRAQVSTWRR
                     TDDNAIPARGKITGAYINGALAKSEAQLNGYDEAIVLTQDGHVSEGSAENLFLVKAGR
                     LITPPVTDNILEGITRRRLMAVARERLELQVDERSLDRTELYTADEVFLCGTGAQISP
                     VVEIDRRTIGNGRPGPVTRQLHDLYFDAVRGRLDEYRDWLTPVY"
     gene            complement(3552..>4362)
                     /locus_tag="VHK28_02130"
     CDS             complement(3552..>4362)
                     /locus_tag="VHK28_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141060.1"
                     /translation="RRGTGMKVNVRILGRDVAFFMFGYAIAIGTAFLPQELAILKWVA
                     AGALFIIYAVYVRLHFTDAPEEGDPETLNRLHATRLPGMAGRHTLDIDILPGQAHERE
                     HLEGATPRMRIIVSQVVLALGMIIVGAQVFVGAVEHISVVIGLDPTILALIIAPIATE
                     LPEKFNSVLWVRTGKDTLAMGNITGAMVFQSCLPTVLGLLFTTWTFTAQSAISFLSAG
                     VAFLATIIIFGTMLRSGRLSAWTLLAGGPLYLVYVYLALFTPLGAGAEGLH"
BASE COUNT          718 a         1462 c         1550 g          632 t
ORIGIN      
        1 tcggcgcggg cgagagcggc ggcgcgctgc gcggcaaggc gctcctcaag gatggcgcgc
       61 tcgatcccgg tcatcacccg cggcccgctc tcggcggcat gctccacggg ctcgatgatc
      121 tcgggcggcg gcggcgggac cacggccggc atgaacgccc gggtcctggc ggtaacctcg
      181 tccaggcgct gctgcacgcg cgccgcggca cccagggcct cgagcagctg tgcctcggat
      241 gccgccgcga taccgaagcg cccctggtcg gtcgggacca ggatcatcga cggtgtcggc
      301 gccagccgga cgagctggat ggactcctcc ccgcggaggc gggcgggccc gagcgcccag
      361 cccaacgcgc cgaagcgagg ccgcagccgg gcggctccct cccccaccag cgggacgcgg
      421 gtgaccgcac cgcgcaccag cgtgaaggct cgctcgccgc cgagccagcg caggcgcagg
      481 gtggccacct ccacgtccag ccgggccgtc agcgggatga gggccaggta gatcgccagg
      541 acggagacga cgatcccggc caccaccagc ccaaccccgg gaaagccgcc gagcaggacg
      601 ccgccgcccg ccgccacagc gcccgcgacc agcagcacga gcggcgcgac cagcagccgc
      661 atgcgggcgc gggccagtgg gattcgggcg atcgcgacgg ttccggcgtc gggcatgcct
      721 acgccttctc ggtgacgcgg tactcgaggt agcggccggt cagcagccgg gtctgggcat
      781 cgagcgccgg caggttgctg aagaagatgc ccacgaaggg caggcccagc cactgcacgt
      841 agctcagcag gcgcttgaag cgcccccacg ctgcgggccg cggcgggacg atgcggtgct
      901 ccacccagat caggaccgcc agggccagca aaccgatgct cagcatggtg gaagcgtaga
      961 acggcaggtt ctgtccgagc gtggtttcgg cgaacgccgg gttgatgacc agcgggatgg
     1021 tcgcgccgaa catgaccacg aacggtgcga tggcccagtt gacgtgctcg ccgaacaggt
     1081 tgagcaggcg ccacacgcgc gacgccagcg ggatctcggg gtgcaccagg atgttgcgca
     1141 ccacgtaggg gatgtcggtc acgccccagg cccatcgtcg ggcctggagg tactggctgg
     1201 ccatcgagcg ccagtaggtg cgagcccgca ccgcgtctcc gtatatcggg atgaagagcg
     1261 ggacggcccg gaaccggtcg ccgtagcgga agaagctctt ccagtagaag cgggtgtcct
     1321 ccggaatggc atcggtcgcc cagtagccga cgtcatgcac caggtgcagg gtggtcgagt
     1381 acgaaccgaa gctctgcagc ttctccggca ggacgctgcg ccacatctgc agctgggtca
     1441 gcaccgcagc gaacaggcgc tgcaggaagg gagcctgcca caggttgttg tggaagtacg
     1501 ggatcggctg gtagagctgt gtctcgcggt tggcatcggt caagtgaacc caggtcaggt
     1561 aggtgaagta ctgcgaatgg acccggtagt cggcgtccag gtcggtgacc aggatgcggc
     1621 gcgcgtccat gccccgctcg cggaccagga ggcggtacaa gtagcggcca ccccaggcca
     1681 tggcgctgct cttgccgatc acgatcccgg gctccagcgg gtcgagtatg tggatgaact
     1741 cggcgaaggc gtggccgaac tcatcgcgca gggcggccac gttggcccgg ccctccgcat
     1801 ccgtctcgcg ggtgatgatg gcgcagatct tccgctccct cggccagtcg gcctcgacca
     1861 gggctcggac cgtgtcgcgc agcttgtcga gcgactcggt gtaggtcggg atgagcgcca
     1921 ggtgcacgta ctcgcgccag tcgggcgggg gctcgggcag ggcgtccagc cgctcgagat
     1981 cggctagctc acgctcgatc acgcgttgtt cgcgcccgcc gggggtggcg cccaaccggg
     2041 cgctggcgcc cgccgcgaat cccagggcct cggccgaggg ccggccagag agcgcggaca
     2101 agcgccggag cagctcctca cggcgtgcga cgggatcgga cagcacgtcc agccgtcggc
     2161 gccagctgac cgcctgcacg gcccggatgc gacggaaggc aacgatcacc gcgcccgcga
     2221 accagagcgc ccggcacagc cagtagaagt cgaagctgag gacgaagtac gccaccagcc
     2281 acggatagct gaagctcagc cagatgggac cgatgatgat cgcccagctg atgatccccg
     2341 gcaccatctc gaggaggcgc tggagccagt gaaatcggtc gggatcgtgc ggcgcggaca
     2401 tctgccctct tggtccgtgt cgccgcggcc gccgcgcccc tggcggccgc gccgtgcgct
     2461 agtagaccgg tgtcagccag tcccgatatt cgtcgaggcg gcctcgcacc gcgtcgaagt
     2521 agaggtcgtg cagctggcgg gtcaccggtc cggggcgccc gttgccgatg gtccggcgat
     2581 cgatctcgac cacgggcgag atctgcgcgc cggtgccgca caggaacacc tcgtcggcgg
     2641 tgtacagctc ggtgcggtcc aggctccgct cgtcgacctg cagctccagc cgctcgcggg
     2701 ccacggccat cagccgccgg cgggtgatgc cctcgaggat gttgtccgta accggcggcg
     2761 tgatcaggcg gccagccttg accaggaaca ggttctcggc gctgccctcg ctgacgtggc
     2821 cgtcctgggt cagcacgata gcctcgtcgt agccattcag ctgcgcctcc gacttggcaa
     2881 gggccccgtt gatgtacgcc cccgtgatct tgccgcgggc cgggatggcg ttgtcatcgg
     2941 tcctgcgcca ggtggagacc tgggcccgga tgccgccctc ggtgtcgatg tactgcccga
     3001 acgggatgcc gaagataacc acgtcggcgt ccaggttgtg gagccggacg ccgatggttt
     3061 cgctggactt gtagacgatc ggtcggatgt acacgtcctc tcggaggccg tcgcggcgaa
     3121 gcatctcgat ggtccgggcc gccagatcct cggcggtgtg cggaatctcc atcatcagga
     3181 accgggccga ggccttcagc cgctcgtaat gcggcaccag gtccaacgcc cacagctggg
     3241 actggtcggc gttccagtag gcacggattc cctcgaagac ggccgtgccg taattgaagg
     3301 catgggtcat gacgctcacg cgcgcgtcgc gcaatgggac gagctccccc cggaagaagg
     3361 cccacagctc gggtgtctgc tcctgtggag cgtcggctgt gcgggtttcc tgcttgagca
     3421 cggatcgctc tcctcgtcgt gcggccacgt ctgcacgggc gcggcaccgg catggccggc
     3481 ggagccagta tacccacgcc ctccctcccg gacgctccgg acgggcccct ggttgcggaa
     3541 tccgtggacc gctagtgcag gccctcggct cccgccccga ggggcgtgaa gagcgccagg
     3601 tagacgtaca ccaggtacaa gggaccgcca gcgagcaagg tccatgccga gagcctgccg
     3661 gagcgcagca tggtgccgaa gatgatgatg gttgccagga aggcgacgcc tgccgagagg
     3721 aacgagatcg cgctctgggc ggtgaacgtc caggtggtga agagcaggcc gagcactgtc
     3781 ggcaggcagc tctggaagac catggccccg gtgatgttgc ccatggccag cgtgtccttg
     3841 ccggtgcgca cccagagcac gctgttgaac ttttcgggca gctcggtcgc gatgggcgcg
     3901 atgatcagcg ccaggatcgt tggatccagc ccgatcacca ccgagatgtg ctccaccgct
     3961 cccacgaaca cctgcgcgcc aacgatgatc atgcccagcg ccagcaccac ctggctgacg
     4021 atgatccgca tgcgcggcgt ggcgccctcc agatgctcac gttcgtgggc ctgaccgggc
     4081 aggatgtcga tgtccagcgt gtgccggccg gccatccccg gcaggcgcgt ggcatgcagc
     4141 cggttcagtg tctcgggatc accctcctcc ggcgcatcgg tgaagtggag ccggacgtag
     4201 accgcgtaga tgatgaacag ggcgcccgcc gccacccact tcaggatggc cagttcctgc
     4261 ggcaggaatg cggtgccgat ggcgatggcg tagccgaaca tgaagaaggc cacgtcgcgg
     4321 ccgaggatgc ggacattgac cttcatgccc gttccgcggc gg
//
LOCUS       DATACA010000100         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_898658, whole genome shotgun sequence.
ACCESSION   DATACA010000100 DATACA010000000
VERSION     DATACA010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898658"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VHK28_02135"
     CDS             <1..1519
                     /locus_tag="VHK28_02135"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2141061.1"
                     /translation="TTKRHPAVIRYRSGISATGELVAQEIEVIMDGGAYCTLTPVVLS
                     RGSLHAAGPYRCENVRIVGRAMATNTPPNGAFRGFGAPQTEFAVETQVNRIAEALDIS
                     PLELRRRWAYREGDITPTGQVLRESVAALDVLETVAEASGFERHRQQTREARKRRTDG
                     ARLASGMGLGLAWHGAGFTGSGEVHLASVASVELTDEGRIRILTASTEMGQGTKTIFP
                     QLVADALGVQYDEVEIAPQDTAIVPDSGPTVASRTAMVVGGLLIRASQKLRAQIEERT
                     GRPFAESYREAAPLRVDERFTPYPGEPFDDKRYTGDAYPTFGWAAAVVQVDVDLDTAE
                     VTVRDYWAADEIGRVIHPILAEGQVEGGSLQAVGYATIEEMKMIDGRYLNDRLATYLI
                     PTALDAPRIHTSLLEKPFSAAPHGAKGVGELPMDVGAPAVVAAIHDAVGAWIHDLPAT
                     PERILAAMTNRPMPPPPGISLENPAPAAPPIEDLPAGGDRIPLRSPTEPPQDFKP"
     gene            1516..1983
                     /locus_tag="VHK28_02140"
     CDS             1516..1983
                     /locus_tag="VHK28_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2141062.1"
                     /translation="MSYRLVVNGEAVELEVPGMRRLLDALREDLGLTGTKEGCGEGEC
                     GACSVLLDGDVVDSCLVPMSQVDGARVETVEGLAAEGRLNELQAAFLETGGAQCGICT
                     PGMLMAGEAFLASGAPPSDEAIREAIAGNLCRCTGYTKIVEAIALAAERRAAP"
     gene            1994..2848
                     /locus_tag="VHK28_02145"
     CDS             1994..2848
                     /locus_tag="VHK28_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX2141063.1"
                     /translation="MPPEPPVHSPHSLDDAYAALGQDGYRPLAGGTDLLVQITGEIGE
                     PPDRVLDIWGLDVLRGIGVEDGALVVGALETYIRIRRSQEVAEHLPALAEAAATIGAA
                     QIQNRGTLGGNAANASPAGDTLPILLATDAELVVGSARGERRVPAAEFWPAYRRTALA
                     ADELLLRVRFPLVDGRQVRFRKVGTRRAQAISKVVMALSWVPQADGTWTDVRVALGSM
                     AATPIRAFATEGALEGAPPTAEAAERAVAALERELKPIDDVRSTADYRRAVAGRVLHR
                     LLRDEGGW"
     gene            2845..3489
                     /locus_tag="VHK28_02150"
     CDS             2845..3489
                     /locus_tag="VHK28_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141064.1"
                     /translation="MTEPGLILYLNGSSSAGKGMLSRAIQRRFAEPFLHIGADTMFEA
                     VEGTGWNQYGRPEDIDAARGDGIRWLLGSDGHITAMEFGEAGRRLIYGLHAMTAALAY
                     EGNNVVVDDVIFEPDMAAHLGRTLAELPAYLIGVRCGLEELERRETERGNRLLGLARL
                     LHERPHEHIPAYDLEVDTARMSAEEAADAVHRFMDRPPKPHAMRALAQLAGGRS"
     gene            3501..>3690
                     /locus_tag="VHK28_02155"
     CDS             3501..>3690
                     /locus_tag="VHK28_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-protection protein"
                     /protein_id="HEX2141065.1"
                     /translation="MPVVRPTRDEVRLFPTAAEFRAWLEENHDRDTHLFVGYYKKGVP
                     KVAMTYAQAVEEALCFGWI"
BASE COUNT          550 a         1298 c         1330 g          512 t
ORIGIN      
        1 cacgacgaag cgccacccgg ccgtcattcg ataccgctcg gggatctcgg cgaccggtga
       61 gctcgtcgcg caggagatcg aggtcatcat ggacggtggc gcgtactgca ccctcacgcc
      121 cgtggtcctc tcgcgcggga gcctgcacgc ggccgggccg tatcggtgcg agaacgtgcg
      181 catcgtgggg cgtgccatgg ccaccaacac gccgcctaat ggtgcctttc gcggattcgg
      241 ggcgccgcag accgagttcg cggtcgagac ccaggtcaac cgcatcgccg aggccctcga
      301 catctccccg ctggagctcc ggcgtcgctg ggcgtaccgc gagggcgaca tcacgcctac
      361 cggccaggtg ctccgcgaga gcgtggctgc gctggacgtg ctcgagacgg tcgccgaggc
      421 atccggcttc gagcggcacc ggcagcagac gcgtgaggcg cgcaagcgcc ggacggatgg
      481 cgcgcgcctg gcatccggca tgggactggg gctggcctgg cacggtgccg ggttcaccgg
      541 gtcgggggag gtgcacctgg cgtcggtcgc atcggtcgag ctgaccgatg agggcaggat
      601 ccggatcctg accgcgtcca ccgagatggg ccagggcacc aagaccatct tcccgcagct
      661 ggtcgccgac gcgctcggcg tacagtacga cgaagtcgag atcgcgcccc aggacaccgc
      721 catcgtcccc gactcgggtc ccaccgtcgc gagccgcacc gccatggtgg tcgggggact
      781 gctgatccgt gcgtcgcaga agctgcgcgc gcagatcgag gagcgcaccg ggcggccgtt
      841 cgccgagtcg taccgagagg ccgccccgtt gcgcgtggac gagcggttca ccccctaccc
      901 cggcgagccg ttcgacgaca agcgctacac cggggacgcc tatcccacct tcggctgggc
      961 ggcggccgtg gtacaggtgg acgtcgacct ggacacagcc gaggtcaccg tgcgcgacta
     1021 ctgggccgcc gacgagatcg gtcgcgtcat ccatcccatc ctggccgagg gccaggtgga
     1081 gggcggatcg ctgcaggcgg tcggctacgc gaccatcgag gagatgaaga tgatcgacgg
     1141 ccggtacctg aacgatcggc tggcgaccta cctgatcccc acggccctcg acgcgccacg
     1201 catccataca tcgttgctcg agaagccctt ctcggccgcg ccgcacggtg ccaagggcgt
     1261 gggggagctc cccatggacg tgggcgcgcc ggcggtggtg gccgccatcc acgacgcggt
     1321 gggtgcctgg atccacgacc tgcccgccac gccggagcgc atcctggccg ccatgaccaa
     1381 tcgaccgatg cctccgccgc ccggcatctc gctcgagaac ccggcgccgg cggccccgcc
     1441 gatcgaggac ctcccggccg gaggcgaccg gataccgctg cgctccccca ccgagccgcc
     1501 acaggacttc aaaccgtgag ctaccggctg gtcgtcaacg gcgaggccgt cgagcttgag
     1561 gtgcccggca tgcgccgtct gctggacgcg ttgcgcgagg acctgggact cacgggcacc
     1621 aaggagggct gcggcgaggg cgagtgcggt gcctgctcgg tgctgctgga tggcgacgtg
     1681 gtcgactcct gcctggtgcc gatgtcccag gtggacggcg ctcgcgtgga gaccgtggag
     1741 gggctggccg ccgagggccg gctcaacgag ctgcaggccg ccttcctgga gaccggcggc
     1801 gcccagtgcg gcatctgcac gcctggcatg ctgatggccg gcgaggcgtt cctggcctcc
     1861 ggtgcgccac cctcggatga ggccatccgc gaggcgatcg ccggcaacct gtgccgctgc
     1921 accggctaca ccaagatcgt ggaggcgatc gccctggccg ccgagcgccg cgccgcgccg
     1981 tagccgcaga cacatgccgc ccgagccgcc ggtccactcc ccgcactcgc tggacgacgc
     2041 ctacgctgcg ctcggccagg acgggtaccg gccgctggcg ggcggcacgg acctgctggt
     2101 ccagatcacc ggtgagatcg gtgagccacc agaccgcgtg ctggacatct ggggcctgga
     2161 tgtgctgcgc ggcatcggcg tcgaggacgg ggcgctggtg gtcggcgcgc tggagaccta
     2221 catccgcatc cgccgctctc aagaggtcgc cgagcatctg ccggcgctgg ccgaggcagc
     2281 ggccaccatc ggggccgccc agatccagaa ccgcggcacg ctgggcggca acgctgccaa
     2341 cgcgtcgccg gcgggcgaca cgctgcccat tctgctggcc accgacgcgg agctggtggt
     2401 cggatcggcg cggggcgagc gtcgcgtgcc ggccgccgag ttctggcccg cctaccggcg
     2461 gacagcgctg gcggccgatg agctcctgct gcgcgtccgc ttcccgctgg tggacggccg
     2521 ccaggtccgg ttccgaaagg tcggcacgcg acgcgcgcag gccatctcca aggtggtcat
     2581 ggccctgagc tgggtgccgc aggcggacgg gacctggacg gacgtccggg tcgcgttggg
     2641 ctccatggcc gccacgccca tccgggcctt cgccaccgag ggcgcgctgg agggcgcccc
     2701 tcccaccgct gaggccgcgg aacgggcggt cgcggcgctc gagcgggagc tgaagcccat
     2761 cgacgacgtg cgatccacgg ccgactaccg gcgtgcggtg gcggggcgcg tcctgcaccg
     2821 cctgctgcgc gacgagggcg gctggtgaca gaaccaggcc tgatcctgta cctcaatggc
     2881 tcgtcgagcg caggcaaggg aatgctgtcg cgcgccattc agcgccgttt cgccgagccg
     2941 ttcctgcaca tcggcgccga cacgatgttc gaggctgtcg agggcaccgg ctggaaccag
     3001 tacggccggc cggaagacat tgatgcggcc cgcggagatg gcatccgctg gctactgggg
     3061 tccgatggcc acatcacggc catggagttc ggggaggcag ggcggcgact gatctacggt
     3121 ctgcatgcca tgaccgcggc actcgcctac gagggcaaca acgtcgtggt ggacgacgtg
     3181 atattcgagc cggacatggc cgcgcacctt gggcgcacgc tcgccgagct gcccgcctac
     3241 ctcattggcg tgcgatgcgg cctggaggag ctggagcgtc gcgagaccga gcgcggcaac
     3301 cgcctgctgg gcctggcccg cctgctccac gaacggccgc atgagcacat cccggcctat
     3361 gacctcgaag ttgacaccgc acgcatgtcg gcggaagagg cagcggatgc cgtgcatcgg
     3421 ttcatggacc ggccaccgaa gccccacgcg atgcgtgccc tggcccaatt ggccggcggc
     3481 cgctcttagg ggacagccgc atgccagtcg tccgtcccac cagggacgag gtgcggctct
     3541 tcccgaccgc cgcggagttc cgcgcctggc tggaggagaa ccacgaccgg gacacgcacc
     3601 tgttcgtggg ctactacaag aagggcgtcc ccaaggtcgc catgacctat gcccaggccg
     3661 tggaggaggc cctctgcttc ggctggatcg
//
LOCUS       DATACA010000101         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2183556, whole genome shotgun sequence.
ACCESSION   DATACA010000101 DATACA010000000
VERSION     DATACA010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183556"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VHK28_02160"
     CDS             <1..833
                     /locus_tag="VHK28_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX2141066.1"
                     /translation="PARAAPDGRPVNGVRGLIESMLSLLREPDVTHIAAATDYVIESW
                     RNDMYPGYKSSAGMDPDLLAQFRDAERALRALGISTWAMVEDEADDALATAVARFADD
                     PRITEVVIASVDKDLAQCVRDGKVVLRDRMRRITYDEPGVLAKFGVSPESIPDYLALV
                     GDSSDGYPGLPGWGARSAAAVLARWRHIDAIPHSALEWGVDVRGAATLARTLADQRDA
                     ALLYRRLAQLNTDAAIDGTLDDLEWLGVPRPAFEALCAELGLESLIGRVHRWAEAPSV
                     "
     gene            885..2102
                     /locus_tag="VHK28_02165"
     CDS             885..2102
                     /locus_tag="VHK28_02165"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX2141067.1"
                     /translation="MPTRFLVAATLAALTLIGSALPGSFAVSRAVAASCSGWTSESQP
                     PPSIRVFRHASGAVETVDFRTYTKNVLSREWIGSWTAESLRSGALAVKSYAWYQVLHW
                     RGGTNASGQCFDLRDDTYDQVYDPSKPTWSTAAAAVDATWGTRVLKNGLIFPTYYNAG
                     ASGEACGANANGWKMYQWGTQGCGLAGKTAAQIMLTYYWPGVTVTDAPAATPSPTPSP
                     SPTAAPTPTPAASPGSTASPTAAPTLQPTPVATAVPTPVPTPVATPLPTPPPVQALPG
                     GGQAGVERAAAPPPPPPANPEPVVVGAVTSPEPARSRFSGSLSVAERSAAAMRRHLER
                     VDDGPVADRGAWAREHVVAAFKAPGAGVMATDVRLVAFGTLWEDAMSRLADALVAQLA
                     GESPSTALNLPGR"
     gene            complement(2132..3202)
                     /locus_tag="VHK28_02170"
     CDS             complement(2132..3202)
                     /locus_tag="VHK28_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX2141068.1"
                     /translation="MSWFPVLLVVHISLAIALLAPSVVLPFLLRGSGSAAAARANPLA
                     RLLLAMQAAVQPVELLDAVEAAYRDVAAGRDRSPLRTHVALPSGDLLLMPGVREGAAG
                     TSVKLVTVTPGNTARGLPTVQAVVAWLDGETGEPTALLDGGTLTRMRTGAASGVATRL
                     LARHDSAVLGLLGAGGQAAWQLRAVLAARPSIEVVRVYSRTAASREAFAAEMAAELGG
                     RVRVEAAASAEEAVRDTDVVCCATTSSTPVFDPGWLAAGTHVNGVGAFRLGMVEIPPQ
                     AFARAALVTVDSREAALAEAGDLVAALEDGVLAADAVEEIGTVPAAWAASREPEALTV
                     FKSVGLAIQDVAAAELILSRLA"
     gene            complement(3260..3595)
                     /locus_tag="VHK28_02175"
     CDS             complement(3260..3595)
                     /locus_tag="VHK28_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141069.1"
                     /translation="MLTTLRRELGALAWILRTLTMVAVVAAVYRELQRPPEERTWHGR
                     LMGKVPYDFRIPTPRRLADAWWNPGSDKVITDQPFGVGWSLNLPALLQRITSLGKQSS
                     PGKRSRAAG"
BASE COUNT          535 a         1426 c         1322 g          509 t
ORIGIN      
        1 gtccggcgcg cgctgcgccg gacgggcggc cggtgaatgg ggtgcgcggc ctgatcgagt
       61 ccatgctctc cctgctgcgc gagccggacg ttacccacat cgcggccgca actgactacg
      121 tcatcgaatc ctggcgcaac gacatgtacc ccggctacaa gtccagcgca ggaatggacc
      181 ccgacctgct ggcccagttc cgcgatgcgg agcgcgccct gcgcgccctg ggaatcagca
      241 cctgggcgat ggtcgaggat gaggcggacg acgccctggc caccgcggtg gcgcgcttcg
      301 ccgacgaccc gcgcatcacc gaggtggtga tcgccagcgt ggacaaggac ctcgcacagt
      361 gcgtgcgtga cgggaaggtc gtcctgcgcg atcggatgcg ccgcatcacc tacgacgagc
      421 ccggcgtgct ggccaagttc ggcgtgtcgc cggagagcat ccccgactac ctcgccctgg
      481 ttggcgacag ctccgacggc taccccgggc tccccggctg gggggcgaga tccgcggccg
      541 ccgtcctggc ccgctggcgg cacatcgatg ccatccctca ctccgcgctg gagtggggcg
      601 tcgacgtgcg cggcgccgcg accctggccc ggaccctcgc cgaccagcgc gatgcggcac
      661 tcctctaccg acgccttgca cagctgaaca ccgacgccgc catcgacggc accctggacg
      721 acctcgagtg gttgggcgtg ccgcgacccg ccttcgaggc gctgtgcgcc gagctggggc
      781 tggaatcgct catcgggcgg gtgcaccgtt gggcggaggc accatcggtc taggaccatc
      841 ggtcggttct ggtgaggtga ggtgcggtgt agcgtgccgc acccatgcca acgcgcttcc
      901 tggtcgcggc cacgctggcc gcgctgaccc tgatcggttc cgccctgccc ggctcattcg
      961 ccgtctcgcg cgcggtcgcc gcatcctgct cggggtggac ctccgagagc cagccaccgc
     1021 ccagcatccg ggtcttccgc cacgccagcg gcgcggtcga gacggtcgac ttccggacct
     1081 acaccaagaa cgtgctatcg cgcgagtgga ttggctcctg gaccgcggaa tcgcttcgct
     1141 ccggggcgct ggcggtcaag agctacgcct ggtaccaggt gctgcactgg cggggcggca
     1201 ccaacgcgtc cgggcagtgc ttcgacctgc gtgacgacac ctacgaccag gtatacgacc
     1261 cctccaagcc gacctggagc acagcggccg ccgcggtaga cgccacctgg gggacgcggg
     1321 tgctgaagaa cggcctcatc ttcccgacct actacaacgc gggggcgtcg ggtgaggcat
     1381 gcggcgccaa cgccaacggc tggaagatgt accagtgggg cacgcagggc tgcggcctgg
     1441 ccggcaagac ggccgcccag atcatgctga cctactactg gccaggggtc acggtgaccg
     1501 atgcaccggc tgcgaccccg agcccgaccc cctcgcccag cccgaccgcc gcacccacgc
     1561 ccacacccgc cgcgagcccc gggtcgaccg cgtcgccgac cgcggcgcca acgctccaac
     1621 ccacgccggt ggcgacggcg gtcccgacgc ccgtgccgac gccggtcgcg acgccgctcc
     1681 cgaccccgcc gccggtccag gcgcttccgg gcggcggcca ggccggcgtc gagcgggcgg
     1741 ccgctccacc acctccgccg cccgccaacc cagagccggt cgtcgtgggg gccgttacgt
     1801 ccccggagcc ggcgcggagc cggttcagcg gctcgctcag cgtggcggag cgctctgccg
     1861 cggcaatgcg ccggcacctg gagcgcgtgg acgacgggcc ggttgctgac cgcggcgcct
     1921 gggcgcgaga gcacgtcgtg gccgcgttca aggcacctgg cgcaggcgtc atggcgaccg
     1981 atgtacgcct ggtcgccttc ggcactctct gggaggacgc catgagccgc ctggccgatg
     2041 cgctcgtggc gcagctcgcg ggggagtccc cgagcacagc gctgaacttg cctggccgtt
     2101 gacaggcgat tcgggcgccc gccgcgagac ctcaggccag ccggctgagg atgagctctg
     2161 cggccgcaac gtcctggatg gcgagcccga cgctcttgaa gacggtgagc gcctccggtt
     2221 cccggctcgc agcccaggcg gcgggcaccg tgccaatctc ctcgacggcg tctgccgcca
     2281 gcactccatc ctccagcgcg gccaccaggt cgccggcctc cgccagcgcc gcctccctcg
     2341 agtcgacggt cacgagcgct gcgcgcgcga aggcctgcgg agggatctcg accatgccaa
     2401 gccggaaggc accgaccccg ttgacgtgag tgccggccgc cagccagccg ggatcgaaca
     2461 ccggcgtcga cgaggtggtc gcgcagcaca ccacgtctgt gtcgcgcacc gcctcctcgg
     2521 cggatgcggc agcctcgacc cgcacccggc cccccaactc ggcggccatc tcggcggcga
     2581 acgcctctcg tgacgcggcg gtccgcgagt acacgcgcac cacctcgatg gaggggcgcg
     2641 ccgccagcac ggcgcgcagc tgccatgccg cctggccacc tgcaccgagc aagccgagca
     2701 cagccgagtc gtgccgggcg agcaggcgcg tggccacccc ggatgcggcg ccggtccgca
     2761 tgcgcgtcag cgtgccgccg tccagcagcg ccgtgggctc accggtctcg ccgtccagcc
     2821 atgccaccac ggcctgcacg gtgggcagcc cgcgggccgt gttgccgggc gtcacggtca
     2881 ccagcttcac cgaggtgccg gcggcccctt cccgcacgcc tggcatcagc agcaggtcgc
     2941 ctgatggcag ggcaacgtgc gtacggagcg gcgagcggtc gcggccggca gcgacatcgc
     3001 ggtacgccgc ctcaacggca tccagcagct cgaccggctg caccgccgcc tgcatggcca
     3061 gcagcagccg ggccagcggg ttggcgcggg ccgcggccgc gctccccgag ccgcggagca
     3121 ggaagggcag cacgacgctg ggagccagca gcgcgatggc caggctgata tggacgacga
     3181 ggaggaccgg gaaccagctc acgggccgat gctacccggt ccgccatggt tcggcggcgc
     3241 gctgatctgg tgtcgatgcc tagccggctg cccgcgagcg cttgcctggg cttgactgct
     3301 tgccgagcga ggttatccgc tgcagcaggg cgggcaggtt gagggaccac ccgaccccga
     3361 aaggctggtc ggtgatgacc ttgtcgctgc ccggattcca ccacgcgtcg gccaggcgcc
     3421 gcggggtcgg gatccggaag tcgtacggca ccttgcccat cagccggccg tgccaggtgc
     3481 gctcctccgg cgggcgctgc agctccctgt agacggccgc cacgacggca accatcgtca
     3541 gcgtccgtag gatccaggcc agtgcgccga gctcgcgccg cagggtagtc aacatgaact
     3601 cgccatcccg caagtcgtgt gccgcggacg gcgtggcatg atgcgcgctg ccgatgcgcc
     3661 ctggccctta tctcgccctg ctccgactga acccggcctt cgcgcgcctg ttcggcgccc
     3721 agctgatcag cttcggcggc gattggttcg ccaccgtcgc catcctcggc ctgtgcctgg
     3781 agctgaccgg ct
//
LOCUS       DATACA010000102         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_657650, whole genome shotgun sequence.
ACCESSION   DATACA010000102 DATACA010000000
VERSION     DATACA010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657650"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1559)
                     /locus_tag="VHK28_02180"
     CDS             complement(411..1559)
                     /locus_tag="VHK28_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2141070.1"
                     /translation="MPVPATALDHESTIVAVVELSARSWLVGAQVAGVPRQCRQKLAP
                     SAAALLEHLDRLRQRAERAGKRVGRVVVAYEAGRDGCWLARWRLGRGIEVDVIQPSSV
                     PVDRRARRAKTDALDVEMLLRTTLAWLRGEPRVGSMVPIPSEAEEDGRRPSRERQDLI
                     KERLALTNKIDGVLATLGVGGYRPLRRDRRECLEALRRPGGGSLPAHAKARLTRLLDR
                     LELVLEQIGVLEEARNAVVARDAVPGGEADGLVRRLAGVKGIGPELATLLTWEAFVRP
                     FRDRRALAAYAGLTGTPFDRGDRRREQGIGKAGNRRLRAALVELAWLWLRYQPDSALA
                     AWFRRRTQAAGGRTRKVMIVALARKLLVALWRFCRDGVLPEGAALKAV"
BASE COUNT          254 a          647 c          546 g          245 t
ORIGIN      
        1 gcaagatcgc gctcaacttc gtccgggccg atggttcact gaaggcgagc ctcaagggca
       61 agcgcaggtg cgcggcctgg gatgacgctt tcatggcgaa gctgatcgcc gggtgatctc
      121 gtgcgtgagc cctcgtatga ggcgcctgct gtcaagcggg aactccctcg tgatcgatcc
      181 aaggtggagc tgcatgttgt cgagaacggg cgatgcccgt ccaacctcgt atccggtcgg
      241 atcggctgat ccgtgaccac gatcccgggc atccgggagg gcagctccaa ggcggcggca
      301 ccattctgag taacggcgcc tgagcgggtc ctgggctaac gacggcgatt cgccgacctt
      361 ggatcggtgc gagggggcct gccgccgagc agacggcgcc tcctcggtct ctaaacggcc
      421 ttcaacgccg cgccctcggg caggacgccg tcgcggcaga agcgccacaa cgcgacgagc
      481 agcttcctgg ccagggccac gatcatcacc ttgcgggtgc gcccacccgc ggcctgcgtc
      541 cgacgccgga accaggcggc gagcgcacta tccggctggt agcgcaacca cagccaggcc
      601 agctcgacca gggccgcgcg caggcggcgg ttgcccgcct tgccgatgcc ttgctcgcgt
      661 cgcctgtcac cgcggtcgaa gggagtaccg gtcagccccg cgtaggcggc cagcgcccgg
      721 cggtcgcgga acggtcgcac gaacgcctcc caggtcagca gcgtggccag ctccgggccg
      781 atccctttca cgcccgccag ccggcggacg aggccgtccg cctcgccgcc cgggaccgca
      841 tcccgggcca cgacagcatt ccgcgcctct tccaggaccc cgatctgctc gagcaccagc
      901 tccagccggt cgaggagacg ggtcaatcgg gccttggcgt gcgccggcag cgagccaccg
      961 cccggtcgac gcaacgcctc caagcattcg cgccggtcac gacgcagcgg ccggtagccg
     1021 ccgaccccga gtgttgccag cacgccgtcg atcttgttgg tgagcgccaa ccgctccttg
     1081 atcaggtcct gccgctcgcg cgagggccgg cgaccatcct cctccgcctc gctcgggatc
     1141 ggcaccatcg agcccacccg cggctccccc cgcagccagg ccagggtcgt gcgcagcagc
     1201 atctccacgt ccagggcgtc ggtctttgcc cgtcgcgccc ggcggtcgac gggcacgctc
     1261 gagggctgga tcacgtccac ctcgatcccg cgcccgagcc gccaccgcgc gagccaacac
     1321 ccgtcccgac ccgcctcgta ggcgacgacc acccgcccga cccgcttgcc ggcccgctcg
     1381 gcgcgctgcc gcaaacggtc caggtgctca agcagcgccg ccgcggacgg ggcaagcttc
     1441 tgccggcatt gccgcggcac gcccgccacc tgcgcgccca ccagccagga gcgtgcgctc
     1501 agctccacga ccgcaacgat cgtgctctca tgatccagcg ccgtggccgg caccggcatc
     1561 cgcgcgttgc ccattgggag tcccctcggt cggtttcaca acgaccggga gtcaacccgt
     1621 tcgtcgctcg cctccatagg ttctggtatg gacgggatca ccatgcttca ggccgcatga
     1681 ctttctgatc ct
//
LOCUS       DATACA010000103         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_416481, whole genome shotgun sequence.
ACCESSION   DATACA010000103 DATACA010000000
VERSION     DATACA010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416481"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VHK28_02185"
     CDS             complement(<1..323)
                     /locus_tag="VHK28_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141071.1"
                     /translation="MPRLIPELDPNGRRLMVAKLFRGFAFGLNSVVLGLYLSELDLAP
                     EVIGLVLSAALAGTLALTLVIALWGDRIGRRRLLVVGSALMVLAAVIPFVGANPVLLV
                     LIGLTG"
     gene            581..>1532
                     /locus_tag="VHK28_02190"
     CDS             581..>1532
                     /locus_tag="VHK28_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141072.1"
                     /translation="MIGSRTSGVALLAAFLPAVVLVACGKVPPPVGGTAERNGLVLET
                     RLRATAESLVVETLVSNQRAEPIHLVPDQCGRITEVTLGRTVFEPEGEQWEGSVHAVK
                     ELILRDQLSRQRPDRFHPRIPGSDSPDPPGCERPEQPVTLEPDGGIVERWELPFDITS
                     ARVLRAVGSSGSVVRAEVVEARDRDELEFLDIYSTGAPEAAEARRGRAVWVETPAEAV
                     VKRQPDAQPAALSLGQLFDRLLEDAKLRAWIEAQPADSWRHAALTPTFAALGDVEEQR
                     VELRLITAEYERAALVNARHDGGGAELQLPGEEWRTRAFER"
BASE COUNT          229 a          538 c          571 g          194 t
ORIGIN      
        1 ccggtaagac cgatgagcac cagcagcacc gggttggcgc ccacgaacgg gatgacggcc
       61 gccagcacca tgagcgccga gccgacgacc agcagcctgc gccgcccgat gcgatctccc
      121 cacagcgcga tcaccagggt cagggccagg gtgcccgcca gggctgccga caggaccagc
      181 ccgatgacct ccggggccag gtccagctcg gacaggtaga ggcccagcac caccgagttc
      241 aggccgaagg cgaagccgcg gaacagcttg gcgaccatca gccgcctccc gttcgggtcg
      301 agttcgggga tgaggcgcgg catcggagca gtatcggggg tcagctgcgg cgccgttcgg
      361 cgtccactgg tgtacgcgtc cagcgggtga cacgaccggc ggtgcctcgc tggccccgtc
      421 ccgtccgccg ccccacctcc acagcgcgtg tcgaggcgag cggtcgatcc ggattgccgg
      481 ttcgaccgcc ccctcacccg cccggcgggt agcggggcga aatgcgggaa tccatcggtc
      541 ctgttggcac ccccagccgc gcgtaggaca cttcggcgct gtgattggat ctcgaacgag
      601 cggcgtcgcg cttctagcag ccttcctgcc ggccgtcgtg ctggtggcct gcgggaaggt
      661 cccgccgcca gtcggcggca ccgccgagcg gaacggcctt gtgctggaga cccggctgcg
      721 agcaacggcc gagagcctgg tggtcgagac cctggtcagc aaccagcggg ccgagccgat
      781 ccacctggtt ccggaccagt gcggacgcat caccgaggtg acgctgggcc gcaccgtgtt
      841 cgagccggag ggtgagcagt gggagggatc ggtccacgcg gtgaaggagc tgatcctccg
      901 cgaccagctg tcgcgccagc ggccggaccg cttccacccg cgcatcccgg gcagcgattc
      961 gcccgatccc cccggatgcg agcggccgga gcagccggtc acgctggagc cggacggcgg
     1021 gatcgtggag cgctgggagc tgccgttcga tatcaccagc gcccgggtgc tgcgcgcggt
     1081 cggctcgtcc ggcagcgtgg tgcgcgccga ggtggtcgag gcgcgggacc gggacgagct
     1141 cgagttcctg gacatctact cgaccggcgc accggaggca gccgaagcgc gccgcggacg
     1201 tgccgtctgg gtcgagacgc cggccgaggc ggtcgtcaag cggcagccgg atgcgcagcc
     1261 cgccgccctc agcctgggcc agctgttcga tcgactcctg gaggacgcga agctgcgcgc
     1321 ctggatcgag gcgcagccgg ccgacagctg gcgccacgcc gcgctgacgc cgaccttcgc
     1381 tgccctgggc gatgtggagg agcagcgcgt ggagctgcgg ctgatcaccg ccgagtacga
     1441 gcgcgcggcg ctggtcaacg ccaggcacga cggcggcggt gccgagctgc agctgcccgg
     1501 cgaggagtgg cgcacgcgtg ccttcgagcg gc
//
LOCUS       DATACA010000104         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1541515, whole genome shotgun sequence.
ACCESSION   DATACA010000104 DATACA010000000
VERSION     DATACA010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541515"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VHK28_02195"
     CDS             complement(<1..351)
                     /locus_tag="VHK28_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141073.1"
                     /translation="MTVAADLVARPLTASRWPDLERLFGPNGAYSNCWCAFQRVSGAE
                     FSRGCENGGTGNRALLQRLTDERRRPGLIAFDGRQPVGWVSVGPRVEFGRILRSPVTR
                     LSPEERSDPSVWSVV"
     gene            complement(348..905)
                     /locus_tag="VHK28_02200"
     CDS             complement(348..905)
                     /locus_tag="VHK28_02200"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEX2141074.1"
                     /translation="MDMISTANPELELVADFVNTNDIEGRRDHLGTPEQLSEWLAERG
                     VAPAGSRVSPELHRRAIAVREGIRALGRANNGEPPRAEQLEAMNRAAAAYPVRIGLTD
                     GEWSLAPMGSGVDAFLARVMAAVARAMADDTWSRVKSCRNDRCRWLFIDHSRNHSATW
                     CSMRVCGSRSKARAYRARRREAAEA"
     gene            994..1161
                     /locus_tag="VHK28_02205"
     CDS             994..1161
                     /locus_tag="VHK28_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141075.1"
                     /translation="MTRRERKKMVDMLDYRLHYAVMATEDRVREHSGRRPANPLRPRL
                     LLRRENSRRRG"
     gene            complement(1199..1789)
                     /locus_tag="VHK28_02210"
     CDS             complement(1199..1789)
                     /locus_tag="VHK28_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141076.1"
                     /translation="MTQEPRSMPPEPRRSAASGPALLGLILIGLGVLFLIGQVVDVDW
                     GGFTWPFFVIIPGLAMVAFGLSQRDGSGLTVAGSMVAIVGLLLLYQNMTDHWESWAYA
                     WALVAPGGSGVGMALYGARSGNAGMTRGGMWQIVTALGIFAVGFVFFEGLIGISGDRL
                     ALPEWVMPAAIIGLGVLVLIRAVTSGRERHDDAQAS"
     gene            complement(1838..2155)
                     /locus_tag="VHK28_02215"
     CDS             complement(1838..2155)
                     /locus_tag="VHK28_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141077.1"
                     /translation="MTDRAVHLLRTLRQEAELPDSTGVRVYSESDESGQKMLSIGFTP
                     DPAPSDEIAERDGLRLFVASDVANPLSEAVMDVVGENGESQLVFRPAADENGATGSTA
                     PEA"
     gene            complement(2208..3197)
                     /locus_tag="VHK28_02220"
     CDS             complement(2208..3197)
                     /locus_tag="VHK28_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2141078.1"
                     /translation="MASPPNLHDVIAARHRLRGVALHTPLERSPRLADESGAAEVRLK
                     LEMLQPTGAFKLRGAYNKVALIAAEQPGAALVTASTGNHGIAVATAAAHHGMPVTVLV
                     GASITTGKLERLRAFESDRCVVEVFGRSPEDAEAEARRRHDAGRAVYVSPYNDADVIA
                     GQGTLAAEVLDDWPECDALVVPVGGGGLICGIGLYAKAVRPDLRLVGVQPSASPPVYA
                     YLQSGSTKPMPIAPTLADAVSGNNERDSITWKMVRQLVDEVVLVDEPQIAEAMGWVID
                     THRLLVEGSGALAVAALRAGLGGLRGRRVTAVLSGRNVDYGTIAGILATYLHA"
     gene            complement(3233..3640)
                     /locus_tag="VHK28_02225"
     CDS             complement(3233..3640)
                     /locus_tag="VHK28_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141079.1"
                     /translation="MLAERAPDRYGQRVTQSVQPREWRRFGFGGPPEPWAHDAQRDLD
                     RLATSYYLEVLELRRQVIGDAPQADVRARVEELFTTATRHKHEIDYTLRHWATPVERA
                     RVEDRLGSLMRISRSMRTLVDAPMPDGWTPDAA"
BASE COUNT          634 a         1447 c         1338 g          576 t
ORIGIN      
        1 gaccaccgac cataccgatg gatccgaacg ttcctcgggc gagagacgcg tcaccggcga
       61 gcggaggatg cgaccgaact caacgcgagg gccgacgctg acccaaccga ccggctggcg
      121 gccgtcgaac gcgatcaggc caggtcgccg ccgctcgtcg gtcagccgct gcagcagcgc
      181 gcggttcccg gtgccgccgt tctcgcagcc gcggctgaac tccgcgccgc tcacgcgttg
      241 aaaggcgcac cagcagttgg agtaggcccc gttggggccg aagaggcgct cgaggtccgg
      301 ccagcgggaa gccgtcagcg gccgagccac gaggtcggcc gccaccgtca tgcctccgcc
      361 gcctcccgcc gccgcgcgcg ataggcgcgg gccttggagc ggctgccaca cacgcgcatg
      421 ctgcaccacg ttgccgaatg gttgcgagag tggtcgatga acagccaccg acaccgatcg
      481 ttgcggcagg acttcacgcg tgaccaggtg tcgtcggcca tggcgcgcgc gaccgccgcc
      541 atgaccctgg ccaggaaggc gtccaccccg gaacccatcg gcgccagcga ccattcgccg
      601 tcggtcaggc cgatccgcac cgggtacgcc gccgcggccc gattcatggc ttcgagctgt
      661 tcggcacgcg gtggctcgcc gttgttcgcg cgaccgagcg cccggatccc ctcccgaacg
      721 gcgatcgctc gacggtgcag ctccgggcta acccgcgagc cggcaggcgc cacgccgcgc
      781 tcagccagcc actccgagag ctgctcgggc gtgcccagat ggtcgcgtcg cccctcgatg
      841 tcgttggtgt tgacgaagtc ggcgaccagc tcgagctcag ggttcgcggt gctgatcatg
      901 tccatgtccc cattgtacat caccactcac aggccttgac aggtgacgca ccatcccgta
      961 gtctacgagt taccagcaaa gggtcaatga ctggtgacga gaagggaacg aaagaagatg
     1021 gtcgacatgc tggactaccg actgcactac gccgtgatgg ccaccgagga ccgcgtcagg
     1081 gagcactccg ggcgccgtcc cgcaaatccg ctacggccac gcctgctgct gcgccgcgag
     1141 aattcaaggc gtcgaggatg acgccccgcc acctgggcag cgtcggtcgc gaacgaggtc
     1201 agctcgcctg cgcgtcgtcg tgacgctcgc gaccggacgt caccgcccgg atcagcacca
     1261 gcacgcccag gccgatgatg gcggccggca tcacccattc ggggagcgcg agccggtcac
     1321 cactgatgcc gatcaacccc tcgaagaaga cgaagcccac cgcgaagatg cccagcgcgg
     1381 tgacgatctg ccacattccg ccgcgcgtca tgcccgcgtt gcctgaccgc gcgccgtaca
     1441 gcgccatgcc gaccccgctg ccgccgggtg caaccagcgc ccaggcgtag gcccagctct
     1501 cccagtggtc ggtcatgttc tggtagagga gcagcaggcc gacgattgcc accatgctgc
     1561 cggccacggt caggccggag ccgtcccgct ggcttaggcc gaacgcgacc atcgccagcc
     1621 cggggatgat cacgaagaac ggccaggtga agccgcccca gtcgacgtcg accacctggc
     1681 cgatcaggaa caggacgccg aggccgatga ggatcagccc caacagcgcg ggtcccgaag
     1741 ccgccgagcg gcgcggctcg ggcggcatcg atcgtggttc ttgcgtcacg gcggcatggt
     1801 acgtctcgtg tcacggcccc gggaagggcc gagcggccta cgcctcaggg gccgttgagc
     1861 ccgtggcgcc gttctcatcg gccgccgggc ggaagacgag ctgcgactcg ccgttctcgc
     1921 cgacgacgtc catgaccgcc tcactcagcg ggttggccac gtcgctggcc acgaacaacc
     1981 ggaggccatc gcgctccgcg atctcgtcgc tcggcgcggg gtccggcgtg aagccgatgg
     2041 acagcatctt ctggcccgat tcgtcggact cgctgtagac gcgcaccccc gtcgagtccg
     2101 ggagctcggc ctcctgccgc agggtgcgga gcaggtggac ggcgcgatcg gtcatctgaa
     2161 gcatgttcgg catcgcctcc aagcatggga attgggcaga agccgattca ggcgtgcaaa
     2221 tacgtggcca gaatccccgc gatcgtgccg tagtccacat ttcggcccga cagcacggcc
     2281 gtcacgcgac gcccgcgcag tccgccaagc cccgcgcgca gcgccgccac cgccagcgcg
     2341 ccgctgcctt ccaccagcaa ccgatgggtg tcgatcaccc agcccatcgc ctcggcgatc
     2401 tgcggctcgt cgaccagcac cacctcgtcc accagctgac gcaccatctt ccaggtgatg
     2461 ctgtcccgct cgttgtttcc ggacaccgca tcggccaggg tcggggcgat gggcatcggc
     2521 ttggtcgagc cggactggag gtaggcgtac accggtggcg acgccgatgg ctgcacgccg
     2581 accaggcgca ggtcggggcg caccgccttg gcatacaggc cgataccgca gatcaggccg
     2641 cccccaccca ccggcaccac cagggcgtcg cactccggcc agtcgtccag gacctcggcc
     2701 gccagcgtgc cctggccggc gatgacgtcc gcatcgttgt agggcgagac gtacaccgct
     2761 cggcctgcat cgtgtcgccg cctggcctcg gcctcggcgt cctccgggct gcgcccgaag
     2821 acctcgacca cgcaccgatc agactcgaag gcacgcagcc gctccagctt gccggtggtg
     2881 atggatgcgc ccaccaggac ggtgacgggc atgccgtggt gtgccgcggc ggtcgccacc
     2941 gcgatgccgt ggttgccggt cgacgccgtg accagcgcgg caccgggctg ctcggcggcg
     3001 atcagcgcca ccttgttgta cgcgccgcgc agcttgaacg ccccggtcgg ctggagcatt
     3061 tccagcttca ggcgcacctc cgcagcacca ctctcatcgg ccaggcgcgg cgaccgctcc
     3121 agtggcgtgt gcagcgccac gccgcgcagc cggtgccgcg cggcgatcac gtcatggagg
     3181 ttcgggggcg atgccacgcg ccgagtctat cggcgcctgc aacgagggag gctcaggcgg
     3241 cgtcgggcgt ccagccgtca ggcatgggtg catccaccag ggtgcgcatg ctgcggctga
     3301 tgcgcatcag ggaccccaac cggtcctcga cgcgggcgcg ctccacgggc gtcgcccagt
     3361 ggcgcagggt gtagtcgatc tcgtgcttgt ggcgggtggc cgtggtgaac agctcctcga
     3421 cacgggcgcg gacgtccgcc tgcggggcgt cgccgatcac ttgccggcgc agctccagga
     3481 cctccaggta gtaggaggtc gccaggcggt ccaggtcccg ctgggcatcg tgcgcccacg
     3541 gctccggcgg gccgccaaag ccgaatcgtc gccactctcg gggttgcact gattgcgtca
     3601 cgcgctgacc gtatcggtcc ggcgctcgct cggccagcat ccgtccgggc tatcgaggcg
     3661 ggtactcctg gcgggaggcc ggtagcacct tgtatggccc gaatggagcc ggaaggttcg
     3721 gtccgcgcga tcccggcgct gtatggaacg cccgggagcg tgagcgccgc cgcagagcgc
     3781 gcctcatgcc gaccatcgac cgtcttatgc tccaaatcca gcgcgcggat tgggtcgcgc
     3841 gcctttcgcg ctctatggcg gtcaggaccg gaaatccaaa aatggtccga tggctgccac
     3901 cttccacagg tatggcaccc tgcgcaacct gcaagcggct ctaccggcca cggccggaga
     3961 tccccgtccc ggcccgcgcc ttctgcagcc gggac
//
LOCUS       DATACA010000105         3880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1942987, whole genome shotgun sequence.
ACCESSION   DATACA010000105 DATACA010000000
VERSION     DATACA010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942987"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..585
                     /locus_tag="VHK28_02230"
     CDS             202..585
                     /locus_tag="VHK28_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141080.1"
                     /translation="MQRPTGITILAVLAAIGGILGLFAALAALGIGAAVGASGLGGLV
                     LIVGLIALAYAVLSLAVAYGFWTLQPWAWPLGVAVQVLGIIQALGQFTSDQTNFVSLV
                     ISLAISGFILWYLFQPHVKAAFGRT"
     gene            670..999
                     /locus_tag="VHK28_02235"
     CDS             670..999
                     /locus_tag="VHK28_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEX2141081.1"
                     /translation="MKLVSAIVHNEDAANLVDALLEREFRATRLHSSGGFLKQGNATI
                     LVGVEDDQVDAVLEIVSANCHSRKQFVNPMPPIMEPGEFYMPYPVEVEVGGATVFVLP
                     VERFERL"
     gene            1008..1586
                     /locus_tag="VHK28_02240"
     CDS             1008..1586
                     /locus_tag="VHK28_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2141082.1"
                     /translation="MPPPAWLEALGGRAPDVVAAWRAVDAGPMAMRRELRRRAGAGSA
                     GRVLAQQEAVADALAAALEACGDDQLGEPGGESDWNVAQAFAHTTAARRWLPRAAALA
                     VSGRWPDEAPILTPGVPGRADASRELLRTYLDKSRRSMAESAATIAGHEEEPCPLDHP
                     LVGRLRCGEWLLWAGVHDVMHLEQLHRLIGDD"
     gene            1579..2718
                     /locus_tag="VHK28_02245"
     CDS             1579..2718
                     /locus_tag="VHK28_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141083.1"
                     /translation="MTERPAPLIGVVDLGAAPTPAFGSYASVARTGQELLAAELSRRL
                     GAMGCAVVRLPVAAVRERPFHWGGWFATALREAVAAARKEGRALDAIGYAGAGSLALA
                     PDSLLDSLASPVAGEVVSNNRFSTDAFVVAGDLDAALDALEACPSDNAAARHLEAAGF
                     RSRDLRSSPWSRFDVDTPQDLALLRLATRLPDTRRLERSVGGFLEMATLPGGRRLEVP
                     NATAIGEVIRDRGAELLVAGRVPASALAHLETEAACRVRALVEERGMRSARDGRPRSL
                     LADWLADRGPAELVRELASLADGVVLDSRVLMAVRAGSSDAAAWPGEEERFASDFGDA
                     GRVGTSWLADLASAAAHAPVPVVLGGHALVSDGLRILVDGAWLGR"
     gene            2887..3246
                     /locus_tag="VHK28_02250"
     CDS             2887..3246
                     /locus_tag="VHK28_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX2141084.1"
                     /translation="MDQTGLIYVLGFGFAATTFVFGTILFSWLITHRRRRPQKGLPYE
                     SGIDTIGDTWSRFGLAFYLYALLFVAFDVEIIFVYLWAITVRAVELPGIIAIGVFLAI
                     LMFGELYAWRKGDLRWH"
     gene            3459..>3880
                     /locus_tag="VHK28_02255"
     CDS             3459..>3880
                     /locus_tag="VHK28_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX2141085.1"
                     /translation="MEEHPKGGFHGLLEVIPTKADYVLDLIRANSLWPLLSGLSCCAI
                     EMMSTATSKNDIDRFGMFPFRASPRQADVLIVAGTLTTKMAGPLVRLWEQMPEPKWCV
                     AMGTCTTSGGRFKRSYSVVQGVDRVMPVDVYVPGCPPRP"
BASE COUNT          498 a         1373 c         1411 g          598 t
ORIGIN      
        1 ccgacgggca gcggcgcccc gtggtgcgcg cctgatgtga atcggaggct tcgtcagctg
       61 gcggcagcgg cgatcacccg ggccgagcgg acatcgccct ggcaggccga gcggacccga
      121 cggctccgcc cccgcgtaca ctcgcgtcgc ggcacggccc gcccttccat tccgccgtgc
      181 cagcgctatg aggaggcgag catgcaacga ccgaccggta tcaccatcct ggcagtgctg
      241 gccgccatcg gcggcatcct gggcctgttc gccgcgctcg cggcactcgg catcggggcg
      301 gccgtcgggg ccagcggact cggcggcctg gtgctcatcg tgggcctgat cgcgctggcc
      361 tacgcggtgc tcagcctggc cgtggcctac ggcttctgga cgctccagcc atgggcctgg
      421 ccgctgggcg ttgcggtcca ggtgctgggt atcatccagg cgctcggcca gttcaccagc
      481 gaccagacca acttcgtgtc gctggtcatc agcctggcga tctccggctt catcctgtgg
      541 tacctgttcc agccgcacgt caaggccgcg ttcggccgga cctgacccgt ggccgccgca
      601 cggcgccctg cggcataatc gcgctgaccg gggaccgcgc cacaccgaac ctgtgaggag
      661 cccgcccaca tgaagctcgt ctccgcgatc gtgcacaacg aggacgccgc caacctcgtc
      721 gacgccctcc tggagcgcga gttccgcgcg acccggctgc acagctcggg cggcttcctg
      781 aagcagggca acgccaccat cctggtgggc gtcgaggacg accaggtgga tgcggtgctc
      841 gagatcgtca gcgccaactg ccactcgcgg aagcagttcg tgaacccgat gcctcccatc
      901 atggagccgg gcgagttcta catgccctac ccggtcgagg tggaggtggg cggcgccacg
      961 gtcttcgtcc tcccggtcga gcgcttcgag cgcctgtagc ctgaccgatg cctcctcccg
     1021 cctggctgga ggcgctcggc ggtcgcgctc ctgacgtcgt agccgcatgg cgtgccgtcg
     1081 acgctgggcc gatggccatg cggcgcgagc tgcgccggcg tgccggcgcg ggcagcgcgg
     1141 gccgggtgct ggctcagcag gaggcggtgg ccgatgccct ggcggcggcg ctggaggcct
     1201 gcggcgacga ccagctcggc gaacccggtg gcgaatcgga ctggaacgtc gcgcaggcat
     1261 tcgcccacac cacggcggcc cgccggtggc tgccacgcgc cgccgccctg gccgtctcag
     1321 gcaggtggcc ggacgaggcg ccgatcctca cgcccggcgt cccgggacgg gcagacgcct
     1381 cgcgcgagct attgcggacc tatctcgaca agtcgcgccg ctccatggcc gagtccgcgg
     1441 caacaatcgc cggccacgag gaggagcctt gtccgctcga ccacccactg gtcggtcgcc
     1501 tgcgctgcgg ggagtggctg ctgtgggcgg gcgtccacga cgtcatgcac ctggagcagc
     1561 tgcatcgcct gatcggcgat gactgagcgt ccggcgccac tgatcggggt cgtcgacctc
     1621 ggcgccgcgc cgacgccggc gttcggctcc tacgcgtcgg tcgcgcgcac cggccaggag
     1681 ctgctggccg ccgagctgtc gcggcgcctg ggggccatgg gctgcgcggt cgtgcgattg
     1741 ccggtggccg ccgtgcggga gcggccgttc cactggggtg gctggttcgc caccgccctc
     1801 cgggaggcgg tcgctgccgc gcggaaggag ggccgagcgc tggacgccat cggctacgcg
     1861 ggcgccggct cgctggcgct ggcgccggac tcgctgctcg actcgctggc ctctccggtc
     1921 gccggcgagg tggtgtccaa caaccgcttc agcaccgatg cgttcgtggt ggctggcgac
     1981 ctggatgctg ccctggacgc gctggaggcc tgcccatcgg acaacgccgc cgcgcgccac
     2041 ctggaggcag ccgggttccg gtcccgcgac ctgcgttcga gcccctggtc gcgcttcgac
     2101 gtggatacgc cgcaggacct cgccctgctg cggctcgcca cacggttgcc ggacacgcga
     2161 cggctggagc ggtcggtcgg cgggttcctg gagatggcca ccctgcccgg cggccgtcgc
     2221 ctggaggtgc cgaacgccac cgccatcggg gaggtcatcc gcgaccgcgg agccgagctg
     2281 ctcgttgccg gcagggtgcc cgcctcggcg ctcgcccacc tcgagacgga ggccgcctgc
     2341 cgggtgcggg cgctcgtcga agagcgcggc atgcgctcgg cccgggacgg tcgcccgcgc
     2401 tcgctgctgg ccgactggct ggccgatcgc gggccggccg agctggtgcg cgagctggcg
     2461 tcgctggcgg acggcgtggt gttggacagc cgcgtgctga tggccgtgcg cgccgggtcg
     2521 tcggatgcgg cggcttggcc gggggaagag gagcgcttcg catcggactt cggggatgcc
     2581 ggccgcgtgg gcacgagctg gctggcggac ctcgcgtcgg ccgcggcgca tgcaccggtg
     2641 ccggtcgtgc ttggggggca tgcgctggtc agcgacggcc tgcgcatcct ggtggacggc
     2701 gcctggctcg gccgctgacc cggcctccga cgagggcgaa tcggactgct agacttgctt
     2761 cagaacgtac gaaaccattc caggcgcgcg gatcggcacg gtccgcgacg cggatgcagc
     2821 ggagggccgg cctcggcgtc ccgggtgggc tgccgctgcg gagcacaggg ctgacgcgga
     2881 gctgacatgg atcagaccgg cttgatctac gtcctcgggt tcgggttcgc tgccacgacc
     2941 ttcgtctttg gcacgatcct gttctcgtgg ctgatcacac accgacggcg ccgcccccag
     3001 aagggcctcc cctacgagag cggcatcgac accatcggtg acacctggtc gcgcttcggg
     3061 ctggccttct acctctacgc gctgctgttc gtggccttcg acgtggagat catcttcgtc
     3121 tacctgtggg ccatcaccgt gcgcgcggtg gagctgcccg ggatcatcgc cattggcgtg
     3181 ttccttgcga tcctgatgtt cggtgagctc tacgcctggc ggaagggaga cctgcgatgg
     3241 cactagacga ccgaactccg cggcgacgcc ggcccagcaa ggcggcggcc gcgcctgaag
     3301 caaccgatgc ggccgaggca cagaccgccc agatggcggg tccggtgggc aacacgctgg
     3361 ccgtcggtgg cggcaacggt gggccggatg gcaacggcgg cacgccgatc atcatcccgg
     3421 accaggccgc ggacgacgac ctgctccgcc agttcggcat ggaggagcat cccaagggcg
     3481 gcttccatgg cctgctggag gtgatcccca ccaaggcgga ctacgtcctg gatttgatcc
     3541 gggccaattc gctgtggccg ctgctgtccg ggctgtcgtg ctgcgccatc gagatgatga
     3601 gcaccgctac gtccaagaac gacatcgacc gcttcgggat gttccccttc cgcgcctcgc
     3661 cgcgacaggc ggacgtgctg atcgtggccg gcaccctgac caccaagatg gccgggccgc
     3721 tggtccgcct ctgggagcag atgcccgaac ccaagtggtg cgtggccatg ggcacctgca
     3781 ccacctctgg cggccgcttc aagcgcagct acagcgtggt gcagggcgtc gatcgggtca
     3841 tgccggtgga cgtctacgtg cccggctgcc cgccgcgccc
//
LOCUS       DATACA010000106         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_416679, whole genome shotgun sequence.
ACCESSION   DATACA010000106 DATACA010000000
VERSION     DATACA010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416679"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1364)
                     /locus_tag="VHK28_02260"
     CDS             complement(12..1364)
                     /locus_tag="VHK28_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX2141086.1"
                     /translation="MVDATGEAKQPPLRVAFDRRLKLEFHGARITSDGGLLAYRELDD
                     ALGLTTLGVAVLAEGRRGKNIQHRLLALLRQAVYGRLAGYEDVNDAARLARDPAMRAI
                     VGREGLARPAASTSQMGRFETEWLASDANLAALADLSGAWIDRVHGRRPPDGIILDLD
                     SSESPTFGQQEGTAYNGHFRCTCYHPLFLFNQFGDLERCLLRPGNVHGAEDWRSVLEP
                     VLGRYRERGVELYFRADAAFAKPELYETLETEGVQYAIRLPANRVSMARIGHLLTRPV
                     GRPPKEPRVFHASFSYQAQSWSKPRRVVAKIEWHQGELYPRVGFIVTNLKRPAERVVR
                     FYHGRGTAEQWIREGKAALRWTRLSCRAFRANAVRLQLFALAYNLANFLRTLALPDAV
                     AQWSLTTLREKLVKIGARIVRHGRYVVFQLAEVAVPRALFADILRRIDRLRGPPVSAT
                     "
BASE COUNT          243 a          554 c          552 g          225 t
ORIGIN      
        1 gttccgcccc gtcacgtcgc cgacacgggc ggcccgcgca gccggtcgat ccggcgcagg
       61 atatcggcga acagcgcccg cggcaccgcc acctcggcca gctggaacac gacgtagcgg
      121 ccgtggcgca cgatcctggc accaatcttg accagcttct cgcgcagggt ggtcaacgac
      181 cattgggcca ccgcatcggg cagcgccagc gtgcgcagga agttcgcgag gttatacgcg
      241 agcgcgaaca gctggaggcg gaccgcgttg gcccggaagg cgcggcacga cagccgcgtc
      301 cagcggagcg cggccttgcc ctcgcggatc cattgctccg ccgtgccccg accgtggtag
      361 aacctgacca cccgctcggc cggtcgcttc aggttggtga cgatgaagcc gacgcgcgga
      421 tagagctcgc cctggtgcca ctcgatcttg gccaccaccc ggcgcggctt cgaccagctc
      481 tgggcttggt acgagaagct ggcgtggaac acccgcggtt ccttgggcgg ccggcccacc
      541 ggccgcgtca gcaggtggcc gatccgcgcc atcgacacgc ggttggccgg cagccggatg
      601 gcgtactgga cgccctcggt ttccagggtc tcgtacagct cgggcttggc gaacgcggcg
      661 tcggcgcgga agtacagctc gacgccgcgc tcgcggtagc ggccgagcac cggctccagg
      721 acggagcgcc agtcctcggc gccatggacg ttgcccggcc gcaggaggca gcgctccagg
      781 tcgccgaact ggttgaagag gaacagcggg tgatagcacg tacagcgaaa atgcccgttg
      841 taggcggtgc cctcctgctg gccgaaggtc gggctctcgg agctgtcgag gtccaggatg
      901 atcccatccg ggggcctgcg cccgtgcacc cggtcgatcc acgcgccgga gaggtccgcg
      961 agcgccgcca ggttggcatc gctggcgagc cactcggtct cgaaccgacc catctggctg
     1021 gtcgaggccg cgggccgagc gagtccctcc cgcccgacga tggcgcgcat ggccggatcg
     1081 cgggcgagtc gcgcggcgtc gttgacgtcc tcgtagccgg ccagccggcc gtacaccgcc
     1141 tgccgcagca gcgcgagtag ccggtgctgg atgttcttgc cccggcgccc ctcggcgagg
     1201 accgccacgc ccagcgtggt caggccgagg gcgtcgtcca gctcgcggta ggcgagcagc
     1261 ccgccgtccg aggtgatcct cgcgccgtgg aactcgagct tcaggcggcg gtcgaacgcg
     1321 acccgcagcg ggggttgctt ggcttcaccc gtcgcgtcca ccatcccgcg cccacccgtc
     1381 tgcgtctatg ctgctgattc caatagcaga atcagcgcag ggcgtcagct gaaagccgag
     1441 ctatctgggg aatccggggt taaaggcact ggtcctccga ccggagaggg cctagaacag
     1501 cgcccatgac gacgacggac aaggtccaca tcggtatcct cggcgccagc ggctacaccg
     1561 gcgcggagct cctg
//
LOCUS       DATACA010000107         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2666123, whole genome shotgun sequence.
ACCESSION   DATACA010000107 DATACA010000000
VERSION     DATACA010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666123"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..867
                     /locus_tag="VHK28_02265"
     CDS             151..867
                     /locus_tag="VHK28_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141087.1"
                     /translation="MSTNLPPLAEPLDAPEPNGPQPIEPATPQMRLQMLSTEHWSLLA
                     SRNLAWNEAFTRSGMFLSTLSFTVVALALVGQASGFGQEFRLFALVTLPVVLFLGIST
                     IIRLDSANNHDMTCVIGMNRIRAAYLELAPDLDRYFVMGTTDDFAGIETTMANLTGRP
                     FFSHIVAAAPFQITVMNSIVLGTIAALVAIQLGASSAVALGAAAVGFLAMVAGFFLYT
                     RRLLATLMRTYKPAFPARED"
     gene            complement(864..1067)
                     /locus_tag="VHK28_02270"
     CDS             complement(864..1067)
                     /locus_tag="VHK28_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141088.1"
                     /translation="MTYRPPDPETPRPASPDTDMPLEEPPTDVPPGSPPEYSPQGEPG
                     WESPGEQEPLTEPGATPGEEPGS"
     gene            complement(1123..1668)
                     /locus_tag="VHK28_02275"
     CDS             complement(1123..1668)
                     /locus_tag="VHK28_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141089.1"
                     /translation="MPVEQAMEASSVAEVPVVLPSSHRLVTVPVYIRSEEPWAGDLPD
                     GFNRVPNRPALTFEGSALYPELIVVKLLERAGWHAAWRKTWNTVEYWRDIGVTVEPSA
                     LAVNIVDHVSRQAGHAPPWNIVAWHGRQLRLLVSRVGERHRTSGYLANWLDAALRLGI
                     PLACFSVVEHRIEHVPAPRRR"
BASE COUNT          255 a          614 c          597 g          322 t
ORIGIN      
        1 aacccaggtg tcctgcgcct gcttgggcga tcgcttcaac gtatcgggca gctcatcccg
       61 tcctggcatg gtgtggctcc ttccgcgctt tcccgaagtc cctgcacggc tggtgccggg
      121 gggttgcatg ccccgctacg ctgtggcccg atgagcacca acctgccgcc acttgcggaa
      181 ccgctcgacg ctccggagcc gaatgggccg cagcccatcg agccggcgac tccccagatg
      241 cggctgcaga tgctgtcgac cgagcactgg agcctgctcg cgtcgcgcaa cctcgcctgg
      301 aacgaggcct tcacgcgctc aggcatgttc ctctcgaccc tctccttcac cgtcgtggcg
      361 ctggcactgg tgggacaggc atcgggcttt ggccaggagt ttcggctgtt cgcgctggtc
      421 accctgccag tggtgctgtt ccttgggatc agcaccatca ttcgcctcga cagcgcaaac
      481 aaccacgaca tgacgtgcgt catcggcatg aaccgcatcc gggctgccta cctggagctg
      541 gctcccgacc tggatcgcta cttcgtcatg ggtacgaccg atgacttcgc cggaatcgag
      601 accaccatgg ccaacctgac gggccggcca ttcttttccc atatcgttgc ggcagcgcca
      661 tttcagatca ccgtcatgaa cagcatcgtc ctcggcacga tcgcggcact ggttgcgatt
      721 cagctcggcg ccagctctgc ggtcgccctg ggggcggcgg ccgttggatt cctggcgatg
      781 gtcgcgggct tcttcctcta cacccggcgg ctccttgcaa cgctcatgcg tacctacaag
      841 ccggccttcc ccgcgcgaga ggactagctg ccgggctcct ctccgggcgt cgcgcccggc
      901 tcggtgagtg gctcctgctc gccgggtgac tcccatccgg gctcaccctg cggcgaatac
      961 tccggtggcg agccgggcgg aacgtccgtt ggcggctctt cgagcggcat gtcggtgtcc
     1021 gggcttgccg gccgcggcgt ctccggatcg ggtggtcggt aggtcatcgg ttcgcgcctc
     1081 ctggggaacg tccgtgcacc caccgtgcca cggcggcgca agctagcggc ggcgaggtgc
     1141 cggtacgtgc tcgatccggt gctccaccac cgagaagcag gcgagcggga tccccagccg
     1201 aagcgcggcg tcgagccagt tggcgaggta cccgctggtc cgatggcgct cgcccacccg
     1261 tgagaccagc agccgcagct ggcggccgtg ccaggccacg atgttccagg gcggcgcgtg
     1321 gcccgcctga cggctgacgt gatccacgat gttgactgcc agagccgacg gctccactgt
     1381 gacgccgatg tcgcgccagt attccaccgt gttccaggtc ttgcgccagg cggcgtgcca
     1441 gccagcgcgt tccaggagct tgaccacgat cagctccggg tagagggcgg acccctcgaa
     1501 ggtcagcgcc ggccggttgg ggacgcggtt gaatccatcg ggcaggtcac cggcccatgg
     1561 ctcctcgctg cggatgtaga ccgggacggt gaccaggcgg tgtgacgagg gaaggacgac
     1621 gggcacttcg gcgaccgatg acgcctccat ggcctgctcg acgggcacca attgtcgcgg
     1681 gcgcgctcgg cttcggccgc ggccggtcga tccggatcct gatgcagggg tctcgatggc
     1741 caatgctcgc cttgcgtagg gagggacagg gtactccatg gccgcgtg
//
LOCUS       DATACA010000108         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2264904, whole genome shotgun sequence.
ACCESSION   DATACA010000108 DATACA010000000
VERSION     DATACA010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264904"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="malQ"
                     /locus_tag="VHK28_02280"
     CDS             <1..1362
                     /gene="malQ"
                     /locus_tag="VHK28_02280"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2141090.1"
                     /translation="PVPPIQPSPYFPGTRRFRNPLYLRVEEVPGASSLGGRRLETLAT
                     AGRALNDRELIDREAAFGAKMAALEMLWREGAPRRGLAGYRREQGAALRSWARYAVIA
                     ERHGASWSRWPEELRRPSSRAVARFAADHADRVAFHQWIQWLLDQQLARAGNALPLIG
                     DVPIGVDRDGADAWAWQDAMALDVNIGAPPDLFNRAGQDWGLPPFNPAALRAAGYEPF
                     IQTLRAALRHAGGLRIDHAMGLFRLWWIPMGNDPRDGAYVRYPAEELLAIVALESARA
                     GATIIGEDLGTVERRVRRELERRSILSYRLAYFERALPASYPRASLAAVTTHDLPTIA
                     GAWSGSDLEVQRGAGLEPDAEDLATLRRRLARVGGLTRQDAVDEVVLRVHAALAASPS
                     LLVAATLDDALRVERRPNLPGTTHQHPNWSIPLPVPLEELMADPYVRLLAARLRRPAE
                     SRS"
BASE COUNT          196 a          584 c          548 g          223 t
ORIGIN      
        1 ccggtgccgc ccatccagcc cagcccctac tttcccggga cgcgccgctt ccgcaacccg
       61 ctctacctcc gggtggagga ggtgcccggg gcctcgtccc tgggcgggcg ccgcctggag
      121 acgctggcaa ccgctggccg cgccctcaac gaccgggagc tgatcgaccg tgaagcggcc
      181 ttcggggcca agatggcggc gctcgagatg ctctggcgcg aaggtgcgcc acgccgcggg
      241 ctggccggtt accgtcgaga gcagggcgcg gcgctgaggt cctgggcgcg ctacgcggtg
      301 atcgccgagc ggcacggggc gtcgtggagc cgatggccgg aggagctgcg ccgtccatcc
      361 agccgcgcgg tggcgcgctt cgcggccgac cacgctgacc gggtcgcctt tcaccagtgg
      421 atccagtggc tgctcgacca gcagctggct cgtgccggca atgccctgcc gctgattggg
      481 gacgtgccca tcggcgtcga ccgcgacggg gccgatgcct gggcctggca ggacgccatg
      541 gcgctggacg tcaacatcgg tgccccgccg gatctcttca atcgcgccgg gcaggactgg
      601 ggcctgccgc cgttcaaccc ggcagccctg cgcgccgccg gctacgagcc gttcatccag
      661 acgctgcgcg ccgccctgcg gcatgccggc gggctgcgca tcgaccacgc catggggctc
      721 tttcggctgt ggtggatccc gatgggcaac gacccgcgcg acggcgccta cgtccgctac
      781 ccggccgaag agctgctggc catcgtcgcc ctggagagcg cgcgggccgg agcgaccatc
      841 atcggcgaag acctcgggac ggtcgaacgc cgcgtgcgcc gtgagctcga gcggcgcagc
      901 atcctgtcct accgcctggc ctacttcgag cgggcgctgc ccgccagtta cccgcgcgcc
      961 tcgctggccg cggtcaccac ccacgacctg ccgaccatcg ccggggcctg gtccggctcg
     1021 gacctcgagg tccagcgtgg cgcagggctg gagccggatg ccgaggacct ggcaaccctt
     1081 cgccggcggc tggcgcgggt gggcgggctc acccggcagg acgccgtgga cgaggtggtg
     1141 ctgcgggtgc acgccgcgct ggccgcctcc ccgtcactcc tggtggccgc aaccctggac
     1201 gatgcgttgc gcgtcgagcg gcggccgaac ctgcccggca ccacgcacca gcaccccaac
     1261 tggtcgatcc cgctgcccgt gccgctcgag gagctgatgg ccgaccccta cgtcaggctg
     1321 ctggcggccc gcctgcgacg ccccgccgag agccggtcct gaacccgggt tcggccacgc
     1381 ggcggcgtgg catcatgcgc ggcggccgtt cgatccacgc tagagagcca aaagcccgac
     1441 caggaggatg acaccatgcc cgagctcttt ccctggctgc tgttcctgca cgtcttggcc
     1501 gccatcgtgg ccttcggccc gacgttcgcc ttctcgatca tcggttccat g
//
LOCUS       DATACA010000109         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2345409, whole genome shotgun sequence.
ACCESSION   DATACA010000109 DATACA010000000
VERSION     DATACA010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345409"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VHK28_02285"
     CDS             <1..1081
                     /locus_tag="VHK28_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141091.1"
                     /translation="VQEALSRLADPQAAQKDAALTNTARQVSRAATGEEDANQEGDPE
                     QAARDLEELRERVGSMDGSQASDTARQLREAAQGSAAANPDLARQLTEAADALERAAR
                     TGGDHDEQAARDQLQEAADAIRQAGTDRELQRDVARAQSALQDGARQVARAGQPQAGQ
                     PGPSGQPGASGQPGQPGASVQPGQPGASGQPGQPGPSGQPGQPGQPGASGQPGQPGNQ
                     PGNQPGNQPGNQPGTGQQPGAGGGATVRRIPGGGLRTGGFDGPSQGNREFDVTGEDDL
                     FAPFDRVGRPGEPSFIAGQGGDGGTDQTGSGSGVGFDNDSVVPYRSVYDLFRDFANNQ
                     LDRQQVPITLKDLVRDYFSRLEPSD"
     gene            1103..2122
                     /locus_tag="VHK28_02290"
     CDS             1103..2122
                     /locus_tag="VHK28_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2141092.1"
                     /translation="MTEQTTTQPATPHGIEPERFAELALGIEAEVGRFIVGQKQLVRH
                     TLISLLAGSHALLEGVPGLGKTMLIRTLGEALELGFSRIQFTPDLMPADIVGTNIIVE
                     EGGERRFRFQPGPIFSNLVLADEINRATPKTQSALLEAMQEHRVTVAKQQYTLTEPFF
                     VLATQNPLEMEGTYPLPEAQLDRFFFKVDVPFPTEDDLIQIMERTTGAERITVKPVAS
                     GADILAMQTLARDVPIASHVMAYAVRILRASHPDTDGAPQLVRQYVRYGGSPRGAQAI
                     VLGAKIHALLDGRFNVAFSDVQAVAEPALRHRVILNFEGEAEGIGTDSVVRAILAETP
                     TQPAE"
     gene            2142..>2486
                     /locus_tag="VHK28_02295"
     CDS             2142..>2486
                     /locus_tag="VHK28_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2141093.1"
                     /translation="MAASAPHRPGIPPPDEPLFDEGFLRRLEQLELASRRLTAGRMKG
                     ERRSVRRGQSVEFADYRNNTAGDDLRQLDWNVYARLERLFIKLFVEEEDVTVHVLVDA
                     SRSMDFGAPNKLW"
BASE COUNT          416 a          900 c          877 g          293 t
ORIGIN      
        1 ggtacaggaa gcgctctcgc gcctcgccga tccacaggcc gcgcagaagg atgccgcact
       61 gaccaacacg gcgcggcagg tatcccgggc ggcgaccggc gaggaggacg ccaaccagga
      121 gggagacccc gagcaggccg cccgcgacct ggaggagctg cgcgaacgag tcggatccat
      181 ggacggctcg caggcctcgg acaccgcccg ccagctgagg gaggcggcgc agggttctgc
      241 cgccgccaat ccggacctcg cacgccagct caccgaggcc gccgacgcgc tggagcgtgc
      301 ggcgcgcacc ggcggcgacc atgatgagca ggccgcccga gaccagctcc aggaggcggc
      361 cgatgcgata cgccaggccg gcactgaccg cgagctgcag cgcgacgtgg cgcgggctca
      421 gtcggcgctc caggacggcg ctcgccaggt ggcgcgcgcc ggccagccac aggcgggcca
      481 gccgggaccc agcggccagc ccggcgcgtc agggcagcct ggtcagcccg gggcgagcgt
      541 ccagccggga cagccaggag catcgggcca gccgggtcag cccgggccga gcggccagcc
      601 ggggcagccg gggcagccgg gcgcgtcggg gcaaccgggg cagccgggaa accagccggg
      661 caaccaaccc ggaaaccagc ccggcaacca acccggcacg gggcagcagc cgggcgccgg
      721 aggcggcgcc accgtccgtc gcatcccggg cggcgggctg cggacaggcg gtttcgacgg
      781 gccctcgcag ggcaaccgcg aattcgacgt gaccggcgag gacgacctgt tcgccccgtt
      841 cgaccgcgtc ggccgcccgg gcgagccgag cttcatcgcc gggcaggggg gagacggcgg
      901 cacggaccag accggctccg gcagtggcgt cgggttcgac aacgactccg tcgtccccta
      961 tcggtccgtc tacgacctct tccgcgactt cgccaacaac cagctggacc gccagcaggt
     1021 gccgatcacc ctgaaggacc tggtccgcga ctacttctcc cgtctcgagc cgtccgactg
     1081 agcgcacacc agcgagggat tcatgaccga gcagaccacg acccagcccg ccactcctca
     1141 cgggatcgag ccggagcgct tcgccgagct ggcgctgggc atcgaggccg aggtgggccg
     1201 cttcatcgtc ggccagaagc agctggtgcg ccacacgttg atcagcctgc tggccggcag
     1261 ccacgccctg ctggaagggg tgcccggcct gggcaagacg atgctgatcc gcaccctcgg
     1321 cgaggcgctg gagctgggct tcagccgcat ccagttcacg ccggacctga tgccggccga
     1381 catcgtgggc accaacatca tcgtggagga gggcggggag cgccgcttcc gcttccagcc
     1441 cggacccatc ttcagcaacc tggtgctggc cgatgagatc aaccgggcca cgcccaagac
     1501 ccagagcgcc ctgctggagg cgatgcagga gcaccgggtg acggtggcca agcagcagta
     1561 cacgctgacc gagccgttct tcgtgctggc cacccagaac ccgctggaga tggagggcac
     1621 ctatccgctg cctgaggcac agctcgatcg cttcttcttc aaggtggacg tgcccttccc
     1681 caccgaggat gacctgatcc agatcatgga gcgcaccacc ggcgccgaac gcatcacggt
     1741 caagccggtg gccagcggcg ccgacatcct ggccatgcag acgctcgccc gcgacgtgcc
     1801 gatcgcgagc cacgtgatgg cctacgcggt gcgcatcctg cgcgccagcc atcccgacac
     1861 cgatggggcg ccccagctgg tgcgacagta cgtgcgctac ggcggttcgc cacgcggggc
     1921 gcaggccatc gtgctgggag ccaagatcca cgccctgctg gacgggcggt tcaacgtagc
     1981 cttcagcgac gtccaggcgg tcgccgagcc ggcattgcgc catcgggtca tcctcaactt
     2041 cgagggtgag gccgagggga tcggcaccga ttctgtggtg cgcgccatcc tggccgagac
     2101 gcccacgcag ccggccgagt aggccggctc cgccaggacc gatggctgca tcggcccccc
     2161 accgacccgg tatcccgccg ccggacgagc ccctcttcga cgagggcttc ctgcggcgcc
     2221 tcgagcagct cgagctggcc agccgacggc tgaccgccgg ccgcatgaag ggcgaacggc
     2281 gcagcgtgcg gcgcgggcag agcgtggagt tcgccgacta ccgcaacaac acggccggcg
     2341 acgacctgcg gcagctggac tggaacgtct acgcccggct ggagcggctg ttcatcaagc
     2401 tcttcgtgga ggaggaggac gtcaccgttc acgtgctggt cgacgccagc cgctccatgg
     2461 acttcggcgc gccgaacaag ctgtgg
//
LOCUS       DATACA010000110         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2586492, whole genome shotgun sequence.
ACCESSION   DATACA010000110 DATACA010000000
VERSION     DATACA010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2586492"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1230)
                     /locus_tag="VHK28_02300"
     CDS             complement(1..1230)
                     /locus_tag="VHK28_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2141094.1"
                     /translation="MEHIAEVLLQLFAVLVAAMIGNEIFRRLRQPTVVGEILGGLVVG
                     PAVLNIYHITPETQLFAEIGVVLLLFQVGVETRLHELLRVGTTALAVGVLGVLLPFGA
                     GFLAAELAGGDLALAVFLAAALTATSVGITSNVLRDLGGLHTKSGRIILGAAIIDDVL
                     AIMILSVASGVAAGSVSAGRIVSLLVVAVLFIVVVLLGGTRILRRRRALLTDPEFAET
                     PFLPGMILMLGLASLAAVIGLAAIIGAFLAGMVVGESSERHALESEVAPVAAFFTPFF
                     FGYIGAQVDLQGLANVEALMLLLGITALAVGSKFFGAFIGALRQGRWRAVVVGWGMVP
                     RGEVGIVVAGLGLSTGAIESDIYSVVVGIAILTTLIVPPLLPVLLRRAEPQGMPEKEP
                     VVVPAGTEDDDGVREGN"
     gene            1428..3098
                     /locus_tag="VHK28_02305"
     CDS             1428..3098
                     /locus_tag="VHK28_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2141095.1"
                     /translation="MTGGLGLTLLIALLAALVLGAIAQRLRLQPLIGFIAAGLVVGPF
                     TPGVVADRQQILDLADIGIALLMFSIGLRFSLRSLAQGGATLISQAVIQVLLTIGLGY
                     AVATLLGLPWLEALFIGAVASNSSSVVIVKVAGEAVVQGTVHGRMSLIWSTVQDLMTV
                     ALVVLLSSLAGAGEENPFLEVGGATLLAVAFIVAVVVAGSRIFPFLLGLLARYGSREI
                     FIIGVAVLALGTAFSATLVGVSVALGAFVTGIALADSDLTDSVLGEIVPLRELFAAFF
                     FVSLGLLVDPGGLLTGVGLLAAVLAVVMIGKGSLAAAIARAGGTPTNVAVRMGALLAQ
                     NGEFSFVLAVVGLQVGGISEDTFAVLMGVVVATIILLRPLLRGSEWLVARLASRRPDI
                     DQREELAEWRLRRHAVVLGYGRVGRTVVRALVSRGFPYVVIDSDYELVRHTREEGDIH
                     LLFGEAGSPSLLDIAGVDEAFLLVVAIPDALATRQAVAYALRRNPRIEVIARAPSEVE
                     AQQLRQLGVRNVIVAEREVANELMRHTLHRFGVGEREITAMLQRRRET"
     gene            complement(3106..3756)
                     /locus_tag="VHK28_02310"
     CDS             complement(3106..3756)
                     /locus_tag="VHK28_02310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2141096.1"
                     /translation="MDRWVCLDVGETLIDETRIWSLWADAFGIPRLTFFAALGAVVAG
                     GGQHGQTFESFGIPRESWLARLPEVEERYGGFREEDLYADARRALEALDAAGYRVAVA
                     ANQPAVREAQLRALGISPDVLAMSDDIGVAKPDPDFFARILELMGDPEPGAVAYVGDR
                     VDNDVLPALKAGMRAVWLRRGPWGVVQQLPEGVRPSLVVDSLDELVGRIGQAWSEA"
     gene            complement(3761..>4291)
                     /locus_tag="VHK28_02315"
     CDS             complement(3761..>4291)
                     /locus_tag="VHK28_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141097.1"
                     /translation="RPVGHRSAGHPSTASIGAMTDADCALCRGPRTDPELDRVEVWRD
                     DHWRLTMSRHGTILAFAYLEPIRHIPYLADLDGPEAVTFGPTIARASRVLREASGARL
                     VYAYVFGGGIPHLHVHLAPNVPEGVLNTALIGGRVEERKLPSGATEIRSLDHADIPDE
                     EVSAVIERVRAAMTAA"
BASE COUNT          661 a         1495 c         1471 g          664 t
ORIGIN      
        1 ctaattaccc tctcgaactc cgtcatcgtc ctccgtgccg gccgggacga cgactggctc
       61 cttctccggc atcccttgcg gctcggcccg gcgtaggaga accggaagga gcggcggcac
      121 gatcagcgtg gtgaggatgg caattccaac cacgaccgag tagatgtcgg actcgatcgc
      181 gcccgttgac agccccagcc cggcaaccac gatcccgacc tctccgcgcg ggaccattcc
      241 ccagccaacg accaccgcac gccaccgccc ctgccgcaat gcgccaatga aggcgccgaa
      301 gaatttcgag ccgacagcca gcgccgtgat gccgagcagg agcatcaacg cctcgacatt
      361 ggccagtccc tgcaggtcga cctgggcgcc gatgtaacca aagaagaacg gcgtgaagaa
      421 ggccgcgacg ggcgctacct ctgactcgag ggcgtggcgt tcgctggatt cccctaccac
      481 catgcctgcc aggaacgctc cgatgatggc ggccaagccg atcaccgcgg ccagcgatgc
      541 cagtcccagc atgagaatca ttcccggcag gaagggcgtc tccgcgaact ccgggtcggt
      601 gagcagcgcg cgtcggcggc gaaggattcg agtcccgccc agcaggacga ccacgatgaa
      661 cagcacggcc accaccagga ggctgacgat cctgcctgcc gagacgctac cggcggccac
      721 gcccgatgcg acggacagga tcatgatggc cagcacgtcg tcgatgatcg cggcgcccag
      781 gatgatcctg ccgctcttgg tgtgcagacc gcccagatca cgcagcacgt tcgaggtgat
      841 gcccacgctg gtggccgtca gtgccgcggc cagaaagacc gccagagcca ggtctccgcc
      901 ggcgagctcc gcggccagga agcccgcgcc gaacggaagc agcaccccaa ggacgccaac
      961 cgccagcgca gtggtgccga cccgcagcag ctcgtgcagg cgggtctcga cgccaacctg
     1021 gaagaggagg agaacgacgc cgatctcggc gaagagctgc gtctcagggg tgatgtggta
     1081 gatgttgagg accgccgggc ccaccaccag ccctccgagg atctctccga ccaccgtcgg
     1141 ctgtcgaagg cgacggaaga tctcgttgcc gatcatcgcc gcgaccagga cggcgaacag
     1201 ctgaaggagg acttcggcga tgtgctccaa tgcgcgcctc caactcgtac ctgaacggca
     1261 aggaagccgc gcgatgcgca gcctcctccg tcgccacggg tacggggcgt ggcgaacccc
     1321 aatggcgaaa tcggcggtcg ggcgaggata ccggcgccgt tcgaacgagg caaccgcggc
     1381 ggtagcccgg cgacgcgccg gtatgatcgg acggtggagg gactcccatg acgggtggcc
     1441 tggggctcac cctgctcatt gcgttgctgg ctgcgttggt gctcggcgcc atcgcccagc
     1501 gcctgcggct ccagccactg atcggcttca tcgcggctgg cctggtggtc ggtcccttca
     1561 cgcccggcgt cgtcgccgac cgccagcaga tcctggacct ggccgacatc ggtatagcgc
     1621 tgctgatgtt cagcatcggc ctccgtttct cgctccgctc gctggcgcag ggcggcgcga
     1681 ccctgatcag ccaggcagtc atccaggtgc tgctcaccat cggccttggg tacgcggtgg
     1741 cgaccctgct cggcctgccg tggcttgagg cgctgttcat aggcgccgtc gcctccaact
     1801 cgtcgagcgt ggtgatcgtc aaggttgccg gcgaggcggt cgtccagggc accgtgcatg
     1861 ggcgcatgag cctcatctgg tccaccgtgc aggacctgat gacggtggcc ctggtggtgc
     1921 tcctcagctc gctggccggt gccggcgagg agaacccttt cctggaggtg ggcggtgcca
     1981 ccctactggc ggtcgccttc atcgtggctg tcgtggtcgc cgggtcccgg atctttccct
     2041 tcctgctggg tctgctggcg cgctacgggt cgcgggagat cttcatcatc ggcgtggcgg
     2101 tgctggcgct ggggaccgcg ttctcggcca ccctggtggg cgtctctgtg gcactcggcg
     2161 ccttcgtgac cggcatcgcg cttgccgact cggacctcac cgacagcgtc ctgggggaga
     2221 tcgtcccgct tcgcgagctg ttcgccgcct tcttcttcgt gtccctgggc ctgctggtcg
     2281 accctggtgg actgctgacg ggggtagggc tcctcgctgc ggtgctggcg gtggtcatga
     2341 tcgggaaggg gtcattggcc gctgccatcg cgcgcgccgg cggcacaccg accaacgtcg
     2401 ccgtgcggat gggtgccctg ctcgctcaga acggcgagtt ctcattcgtc ctggccgtgg
     2461 ttggcctgca ggtcggcggc atcagcgaag acaccttcgc cgtgctgatg ggcgtggtcg
     2521 tggccaccat catcctgctc cgtccgctgc tccgcggttc cgagtggctg gtggcgcgcc
     2581 tggccagccg ccgtcccgat atcgaccagc gcgaggagct ggccgagtgg cggctgcggc
     2641 ggcatgcggt ggtgctcggc tacggccgag tcgggcgcac cgtggtgcgc gcgctggtgt
     2701 cgcgcgggtt cccgtacgtg gtgatcgact cggactacga gctggtgcgg cacacgcgcg
     2761 aggaaggtga catccacctg ctcttcggcg aagcgggcag ccccagcctg ctggacatcg
     2821 ccggcgtcga tgaggcgttc ctcctggtgg tggccatacc tgacgccctg gcgacgcgcc
     2881 aggcggtggc ctatgcgctg cgccgcaacc cgcggatcga ggtcattgcc agggctccat
     2941 ccgaggtcga ggcgcagcag ctgcgacagc tgggcgtccg gaatgtcatc gtcgcggaac
     3001 gcgaggtggc caacgagctg atgcggcaca ccctgcaccg cttcggcgtc ggcgagcgcg
     3061 agatcaccgc catgctccag cgccggcgcg aaacgtgagg ggagctcaag cctccgacca
     3121 ggcctggccg atgcgtccca ccagctcgtc caggctgtcc accaccagcg acggccgcac
     3181 tccctccggg agctgctgga cgacgcccca cggtccacgg cgcagccaca ccgcgcgcat
     3241 gccggccttc agcgcgggga ggacgtcgtt gtccacgcgg tcgccgacgt aggccaccgc
     3301 ccccggctcc ggatcgccca tgagctccag gatgcgggcg aagaagtccg ggtccggctt
     3361 tgccacgccg atgtcgtccg acatggcgag cacgtccggc gagatcccca gcgcccgcag
     3421 ctgcgcctcg cgtacggccg gctggttggc ggcgaccgcc acccggtagc cggctgcgtc
     3481 gagcgcctcc agcgcgcgcc gagcatccgc gtagaggtcc tcctcgcgaa agccgccgta
     3541 gcgctcctcc acctccggca ggcgcgccag ccacgactcg cgcggaatgc cgaacgattc
     3601 gaacgtctgg ccatgctggc caccaccggc cacgacggcg cccagcgcgg cgaagaaggt
     3661 cagccgcggg atgccgaagg catcggccca cagggaccag atgcgggtct catcgatcag
     3721 cgtctcaccg acgtccaggc acacccatcg gtccatggcg tcaggcagcc gtcatggcgg
     3781 ctcgcacccg ctcgatcacg gcggagacct cctcgtccgg gatgtccgcg tggtcgaggc
     3841 tgcgaatctc ggtggccccg ctcggcagct tgcgctcctc tacccgtcca ccgatgagcg
     3901 cggtgttcag cacgccctcg ggtacgttcg gcgccaggtg gacgtgtagg tgcggaatgc
     3961 caccgccgaa cacgtaggcg tacaccaggc gggcgccgct cgcctcgcgc agcacccggc
     4021 tggcacgggc gatggttggc ccgaaggtga cagcctccgg cccgtccagg tcggcaaggt
     4081 agggaatgtg ccggatcggc tccaggtagg cgaaggccag gatcgtcccg tggcgggaca
     4141 tggtcagccg ccagtggtcg tcgcgccaga cctcgacccg gtcgagctcc ggatcagtcc
     4201 gcgggccacg gcagagtgcg cagtcggcat cggtcatggc accgatgcta gcggtggaag
     4261 ggtggccggc ggagcgatgc cccaccggcc g
//
LOCUS       DATACA010000111         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_256890, whole genome shotgun sequence.
ACCESSION   DATACA010000111 DATACA010000000
VERSION     DATACA010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256890"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHK28_02320"
     CDS             complement(<1..194)
                     /locus_tag="VHK28_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141098.1"
                     /translation="MDQTATETAIPARATRREWIGLAVITLAALVYAMDLTVLNLAIP
                     RISSELQPTSAQLLWMIDIYG"
     gene            complement(291..1319)
                     /locus_tag="VHK28_02325"
     CDS             complement(291..1319)
                     /locus_tag="VHK28_02325"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2141099.1"
                     /translation="MGKPGRRPLELTRDQILAFRRRGQALEGRMPPGGESLRGAAWAG
                     LQDSVPRSALHSLHARVQGVAANALDDPALVQVWGPRYTAFVVPSDAHAAFTLARLPE
                     RGRLRERAQDLAARLDRHLAGRKLLVDEAAHGIGLHDNSLRYASLTGRVQIRWGGARQ
                     PTIWTVPPPDLEPAEALRELVRRYLHWYGPSTVDSFVRWGGIEVRAARSAFDALASSL
                     LEVRTPQGEAHVLAADEPALRGSAEPPAAARLLPSGDPYYLLWGADRELLVPDAAHRA
                     LLWTSRVWPGAVLARGEIVGTWRRARAVVSVSLWRPLSRSEREAVESEASTLPLPEIA
                     AAPLVRWE"
     gene            complement(1322..1990)
                     /locus_tag="VHK28_02330"
     CDS             complement(1322..1990)
                     /locus_tag="VHK28_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2141100.1"
                     /translation="MSEQGWKEFLAADGVEDWVVLHGGAVAVFRVKSFSEAARLAEAI
                     AQVPGLEGSATLLTVASDHLAVRLTRDMWGLEQRHVELARAVSAVIRERGAVADRRAV
                     QEVQLAVAAKPDALDLGFWRAVLGYAPMADDNAVDPLGHGSTVWMQDLNPDKPLRHAM
                     HVDVSVAREHIEARLAAALAAGGRIVDDSSAPASWILSDRAGNRVCIAAWPDGVAPPE
                     PESA"
BASE COUNT          392 a          866 c          880 g          365 t
ORIGIN      
        1 ccatagatgt cgatcatcca cagcagctgc gcgctcgtcg gctgcagctc cgagctgatg
       61 cgcgggatgg ccaggttgag caccgtcagg tccatggcgt agacgagcgc ggccagggtg
      121 atgaccgcga ggccaatcca ctcccgccgc gttgcccgtg ccggtatcgc ggtctcggtg
      181 gctgtctgat ccatgctcca ggtgctcctt ccactgacgc tcatcgatgg tgcttgctgc
      241 ggtaagacga cggcagcggg tcgcagtcat cggtcaggtg gcagttggca ttattcccag
      301 cggacgaggg gcgcggcggc gatctcgggc aacggtagcg tggatgcttc agactcgact
      361 gcctcgcgtt cgctcctcga gagaggccgc caaagcgaga ccgaaaccac ggcccgtgct
      421 cgacgccagg tgcccacgat ctcgccgcgg gccaacacgg cgccgggcca cacacgcgat
      481 gtccagagta gggctcggtg cgcggcatcg gggaccagca gctcacgatc ggcgccccag
      541 agcaggtagt aggggtcacc gctgggcaag agccgcgcgg ccgcgggagg ctccgcagac
      601 ccgcgcagcg ccggctcatc cgcggccagg acgtgcgcct ctccctgggg cgttcgcacc
      661 tctagcagcg aagaggccag ggcatcgaag gcggaacggg cggcccggac ctcgatgcca
      721 ccccaacgga cgaaggaatc gacagtcgac ggtccgtacc agtgcaggta gcggcggacc
      781 agctcgcgca gggcctcagc cggttccagg tccggaggcg ggacggtcca gatggttggc
      841 tgccgagcac caccccatcg gatctggacc cgcccggtca gcgaggcgta gcgcaggctg
      901 ttgtcgtgaa ggccgatccc gtgggccgcc tcgtccacga gcagctttcg accggcgaga
      961 tgccggtcca ggcgtgcggc gaggtcctgc gcgcgctccc tcaggcgtcc cctctccggc
     1021 aggcgggcga gggtgaaggc ggcgtgcgcg tcagagggca cgacgaacgc ggtgtagcgc
     1081 ggcccccaaa cctggacgag ggctgggtcg tccaaagcgt tcgctgctac cccttggact
     1141 cgggcgtgga gcgagtggag ggccgatcgc gggacgctgt cctggaggcc cgcccaggcc
     1201 gcgcctcgca gcgactcccc gccgggtggc atccgcccct ccaatgcctg acctcgacgt
     1261 cggaaggcca ggatctggtc gcgcgtgagc tcgagcgggc gccgtccggg cttacccatt
     1321 gtcaggcgga ttccggctca ggaggcgcca caccatcggg ccaggccgcg atgcagaccc
     1381 ggttgccggc ccgatcactg aggatccacg acgcgggcgc gctcgagtcg tcgacgatgc
     1441 ggccaccggc ggccagcgcc gctgccagcc gcgcctcgat gtgctcacgc gcgaccgaga
     1501 cgtccacgtg catcgcgtgc cgcagcggct tgtccggatt caggtcctgc atccagacgg
     1561 tcgatccatg tccgagcggg tcgacggcgt tgtcgtcggc catgggtgcg tatccgagca
     1621 cggcgcgcca gaagccgagg tccagcgcgt ccggcttggc ggcgacggcc agttgcacct
     1681 cttgcaccgc gcggcggtcg gcgacagcgc cgcgttcccg gatgaccgcg gacacggccc
     1741 gtgccagctc aacatggcgc tgctccagtc cccacatgtc ccgggtgagc cgaaccgcca
     1801 ggtgatcgga tgcgaccgtg aggagcgttg ccgacccctc cagtccggga acctgcgcga
     1861 ttgcttcagc cagccgtgcc gcctcgctga acgacttcac gcggaagact gccaccgcgc
     1921 cgccgtgcaa gaccacccag tcctcgacgc catcggccgc gaggaactcc ttccatccct
     1981 gttcgctcac ctgccatgtt tagcatctac caatgcaccg aagggcgccc gataccgcat
     2041 gaccgaaggg agcatcaggg tcgtcgcggc caacgaggca ggctgtggcg agctggcggc
     2101 cgtgttcggc gagcgaggtc cggcggcgtt ttgccgctgc cagcgctaca agctggcacc
     2161 tggcgaggcg ttctcgaaat tcccggtcga ggagcgcatc caacggcttc gggagcagac
     2221 gcactgcgga gaaccggacg cggctgccag cagcgggctg gtggcctacc tcgatggcgt
     2281 gcccgtgggc tggtgcgcgg ttgagccacg caccgcgtac tccggcctcc tgcgggtcta
     2341 ccgggtgccc tgggaggggt gtgacgagga tcggatggac gccagcgtgt ggcccgtacc
     2401 aggacgcagg tcacggccca cacgctggcg tccatccgat cctcgtcaca cccctcccag
     2461 ggcacccggt agacccgcag gaggccggag tacgcggtgc gtg
//
LOCUS       DATACA010000112         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1622317, whole genome shotgun sequence.
ACCESSION   DATACA010000112 DATACA010000000
VERSION     DATACA010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622317"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="VHK28_02335"
     CDS             <1..1593
                     /locus_tag="VHK28_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX2141101.1"
                     /translation="FRERRRVAVPVIAALGGMILPVVLFLAINAGTEAARGWAMVMAT
                     DTAFAVGVLALVGRRSSMRLRVFLLTLVIVDDVAAVSVIAVAYSTDVQLGALSAGIAL
                     LAAMALLRWRGVERPAVYVVLSLAIWLAASEAGVHPTVAGVAIGMLTGAHPPRRERLQ
                     EATGLTRAFRQQPSPALARAAASRISLSLSPNDRLQHALHPWSSYLVVPLFALANGGV
                     DLSGNLLERVVGSPLVIGIVLGLVVGKTVGISAGAWLATRPWLGGLPLSVGWPSLVAA
                     SSVAGIGFTVSLLIADLSYRGELLDAAKLGILSASLTAGLLSIALFYGLSLLPRDFLR
                     RAEGRTSQPLADLVVPVDPTEDHVIGLEDAPVTLLEYGDYECPHCGRAAPVIRQLLDG
                     HHGEIRFVFRHLPLTDVHPNAALAALAAEAAGAQGRFWEMHEALFAHQDALQLSDVVG
                     YARDLGLDVGTFERDLREARYSAAVERHISSAEAAGVAGTPTFFINEVRYRGAYDLES
                     LERAVGGAGRFASNQALAAEGG"
     gene            1613..>2482
                     /locus_tag="VHK28_02340"
     CDS             1613..>2482
                     /locus_tag="VHK28_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2141102.1"
                     /translation="MSIEPAAEPRPDLSVGTGTPDRFLSSKIRAMPPSGIRRFFDMLA
                     EMKEVISLTIGEPDFTTPEPITRAAIASLEAGETHYTANGGMIELRRLIANDLQHRYG
                     LGYDPRTELFITVGASEGLDATLRAVCNPGDEVIYHEPSFVAYAPCIELAGGVPVAVS
                     TTDATDFRVTAEMIEAAVTPRTRAIFLGYPNNPTGAVLEREDLEAIAAVAERHDLLVI
                     TDEIYERLVYGDHAHTPFPSLPGMRDRTVLISGFSKSYAMTGWRIGYVAAPAELMTGI
                     AKVHQYGIMCAPTP"
BASE COUNT          334 a          900 c          863 g          385 t
ORIGIN      
        1 ttccgcgagc gccgacgcgt ggccgtgccg gtcatcgccg ccctgggcgg catgatcctg
       61 ccggtcgtcc tgttcctggc gatcaacgcc gggaccgagg cggcacgcgg ctgggcgatg
      121 gtgatggcca ccgacaccgc cttcgccgtg ggcgtgctcg ccctcgtggg ccgtcgcagt
      181 tcgatgcggc tgcgggtgtt cctgctgacg ctggtcatcg tcgacgacgt ggccgcggtc
      241 agcgtgatcg cggtggccta cagcacggac gtgcagctag gggcgctgtc ggccggcatc
      301 gccctcctgg cggccatggc gctcctgcgc tggcggggcg tcgagcggcc tgccgtgtac
      361 gtcgtactgt cgctggccat ctggctggcg gcgtccgagg cgggggtgca cccgaccgtg
      421 gccggcgtgg cgatcgggat gctgaccggg gcgcacccgc cgcgccgcga acggctccag
      481 gaggcgaccg ggctgaccag ggccttccga cagcagccga gcccggcact ggcgcgcgcc
      541 gccgcctcgc ggatcagcct ctcgctctcc ccgaacgatc gcctgcagca cgcgctgcat
      601 ccgtggagca gctacctggt ggtgccgctc ttcgccctgg ccaacggcgg cgtggatctc
      661 agcgggaacc tcctcgaacg ggtcgtcggc tcgccgctgg tcatcggcat cgtgctcggc
      721 ctggtggttg gcaagacggt tgggatctcg gccggcgcct ggcttgcgac tcgaccgtgg
      781 ctgggcgggc tgccgctctc ggtcggctgg ccatccctgg ttgcggcgtc gtcggtggcc
      841 ggcatcggct tcaccgtctc gctgctgatc gccgacctca gctacagggg cgagctgctc
      901 gatgccgcga agctcggcat cctgagcgcc tcgctgacgg ccgggttgct gagcatcgcc
      961 ctcttctacg ggctctcgct gctgccgcgg gacttcctgc gacgcgcgga gggcaggacc
     1021 tcgcagccgc tggcggacct ggtcgtgccg gtcgatccca ctgaggacca cgtcatcggc
     1081 ctcgaggacg cgccggtgac gctgctggag tacggcgact acgagtgccc gcactgcggc
     1141 cgcgcggcgc ccgtgatccg ccagctgctg gacggtcacc acggcgagat ccgcttcgtc
     1201 ttccgccatc ttccactgac cgatgtccac cccaacgccg cgcttgccgc cctggccgca
     1261 gaggcggccg gtgcgcaggg ccgcttctgg gagatgcacg aggcactctt cgcgcaccag
     1321 gacgcgctcc agctctcgga cgtggtcggc tacgcccgcg acctggggct cgacgtgggg
     1381 accttcgagc gcgatctgcg cgaggcgcgc tactcggccg cggtggagcg tcacatcagc
     1441 tccgccgaag ccgcaggcgt ggccggcacc ccaaccttct tcatcaacga ggtccggtat
     1501 cgcggcgcct acgacctcga atcgctggag cgggccgtcg gcggagccgg ccgcttcgcc
     1561 agcaaccagg cgctggcggc cgagggcgga tgagcaccgc tagactgtgg tcatgagcat
     1621 cgagccggcc gccgagccgc gccccgacct cagcgtgggc acggggactc cagaccgatt
     1681 cctgtcgtcg aagatccgcg ccatgccgcc atcgggcatc aggcgcttct tcgacatgct
     1741 ggccgagatg aaggaggtca tcagcctcac catcggtgag ccggacttca ccacgccgga
     1801 gcccatcacc cgcgccgcga ttgccagcct ggaggcgggg gagacgcact acaccgccaa
     1861 cggcggcatg atcgagctgc ggaggctgat cgcgaacgac ctccagcacc gctacggact
     1921 cggctacgac ccgcgaacgg agctgttcat taccgtcggc gcctccgagg gcctggacgc
     1981 cacgctgcgg gcggtgtgca atccgggcga cgaggtgatc taccacgagc cgtccttcgt
     2041 ggcctacgcg ccgtgcatcg agctggccgg cggcgtgccg gtagcggtgt ccaccacgga
     2101 cgcgaccgac ttccgcgtga ccgccgagat gatcgaggcc gccgtcacgc cccgcacccg
     2161 cgccatcttc ctgggctacc ccaacaaccc gaccggtgcc gtgcttgagc gcgaggacct
     2221 ggaggcgatc gccgccgtcg ccgagcgcca cgacctgctc gtcatcaccg acgagatcta
     2281 cgagcggctg gtctacggcg accacgccca cacgccgttc ccgtccctgc cgggcatgcg
     2341 tgaccggacg gtgctgatca gcggcttcag caagagctac gcgatgaccg gctggcgcat
     2401 cggctacgtg gccgcgccgg cggagctgat gaccggtatt gccaaggtcc accagtacgg
     2461 gatcatgtgc gcccccaccc cg
//
LOCUS       DATACA010000113         8981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_900132, whole genome shotgun sequence.
ACCESSION   DATACA010000113 DATACA010000000
VERSION     DATACA010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900132"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="trxB"
                     /locus_tag="VHK28_02345"
     CDS             complement(<1..1002)
                     /gene="trxB"
                     /locus_tag="VHK28_02345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2141103.1"
                     /translation="MTATVPPTTGTETPEQTDTRHRKVAIIGSGPAGLTAAIYASRAR
                     LEPIVFAGNVPLGQLMITSDVENYPGFPQGIQGPELMQQFREQAERFGTTMVEKDVTR
                     VDFSERPFKLWVGDEQWLADSVIVATGASALWLGLASEEAFRGRGVSACATCDGFFFR
                     DREIAVVGGGDTALEEALFLTRFASKITMLVRRDQFRGSKIMQDRALSHPKIEVRWNT
                     EVTDVRGDMTVTELALRDTVSGEESTLNVQGLFVAIGYKPNTELFAGQLELDPRGYCK
                     TARDGDTASNVEGVFVAGDVHDHKYRQAVTAAGEGCKAAIDAERWLESVGEVEAATAT
                     "
     gene            complement(999..1766)
                     /locus_tag="VHK28_02350"
     CDS             complement(999..1766)
                     /locus_tag="VHK28_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2141104.1"
                     /translation="MGELLAAFAAGVASTAAPCILPLYPAFIAYLTGGAATGNVVLPD
                     GGGVAVAGSPVRRLSPMLAAALVLAGVMSGMLVIAAVVIALSAPLSRMIPVILPAADL
                     LLITLGVLLLAGVNPFARLPQPSATPRAGGAAAGAYLYGLLFAPIAIPCIGPFLLGIF
                     AFSLTIGDALGQLAFFAAYGLGFGVPLFVIGIIGQWRGARLARAMVRHERTIGVLVGL
                     ALVAVGAWDLAVNLPELLRAWGLVRIGPFDRFAMLTS"
     gene            complement(1768..2373)
                     /locus_tag="VHK28_02355"
     CDS             complement(1768..2373)
                     /locus_tag="VHK28_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2141105.1"
                     /translation="MPIALLAVLVLGTACSATTPSASAREATATDGGTASDKPASGTA
                     VASDAPSASQMPALGSDPLHTLVLTDVRDGTEFTLGELAAEKPVLLEAMAIWCTNCRA
                     QMHRVIEAHERADFHSVGIDVDPSELPADLAAYVEEQGFEWPFAMANPEIATALTDRF
                     GNGFRFPPNTPMALLFPDGSIRALDFGGYDVEALLAEIAAG"
     gene            complement(2466..2654)
                     /locus_tag="VHK28_02360"
     CDS             complement(2466..2654)
                     /locus_tag="VHK28_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141106.1"
                     /translation="MDDIYRVPREPHPGEPDELTPDTHPMDDIERPHDAGQGRDDPDE
                     PAPKDLDEPGLADRTPRP"
     gene            complement(2657..3025)
                     /locus_tag="VHK28_02365"
     CDS             complement(2657..3025)
                     /locus_tag="VHK28_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141107.1"
                     /translation="MVEDDPAVAELAREMCVGMGQTAAIYAAPGAFLRDCEAGAPAAV
                     VLDWRLEREVGSAAFMAIRHRFPELPVVCWTATPCDHLPDMVRDDPHTRVVDKADGAG
                     AFEVALRWAVSGLASNGVEA"
     gene            3187..4392
                     /gene="ispG"
                     /locus_tag="VHK28_02370"
                     /gene_synonym="gcpE"
     CDS             3187..4392
                     /gene="ispG"
                     /locus_tag="VHK28_02370"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX2141108.1"
                     /translation="MTRRTTTSVDVGGVSIGSAHPIVVQSMTNTDTADADATAIQVAQ
                     LAHAGSELVRVTVNNDEAAQAVPRIREKLDRLGVGTPLIGDFHYNGHLLLSRYPEAAA
                     ALAKYRINPGNVGAKRRDANFQQIVEVAVANAKPVRIGVNWGSLDQQLLTEMMDANAA
                     LPEPADSREVMIEAMIESALRSARLAEETGLAHDRIIISAKVSGVQDLVEVYGRLAKR
                     CDYPLHLGLTEAGMGSRGIITSTAGLAILLQRGIGDTIRVSLTPSPGGDRTEEVQVAQ
                     QVLQALGLRSFTPQVSACPGCGRTTSTFFQEMARDITAYLAEKMPIWRERHPGVEELK
                     VAVMGCVVNGPGESKHADIGISLPGTFEEPVAPVFVDGQLHTTLRGDDIVPRFLEILD
                     GYVERRYPT"
     gene            complement(4445..4897)
                     /locus_tag="VHK28_02375"
     CDS             complement(4445..4897)
                     /locus_tag="VHK28_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141109.1"
                     /translation="MTERPIPSAARPRHGLTAMLLVLGLALAACGGGGGGGGGGDGDT
                     LTLRVAVVNETEGDAAVSLDVAGTPGEETAIATCSAQVLELDFPAFEDWVLTVAGQTA
                     IDSTQLQDNQLDRNLIAEVWVNEDGSVTQESLAPGRLIGAPAQAGICN"
     gene            complement(4995..5756)
                     /locus_tag="VHK28_02380"
     CDS             complement(4995..5756)
                     /locus_tag="VHK28_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141110.1"
                     /translation="MSDLALAVRQVRYENRAFWRNPAAAFFTFAFPLLFMVIFNVIFA
                     GIDTGDVSASSFFTPAIIVFAVITATYTNIAMTVAIARDNGVLKRVRGTPLPAWAYIF
                     GRIAHAVLIALLLVVIVAAFGALLYDVAVPWDRSPQLLLTLVIAAASFCALGLAVSAL
                     IPNADAAPAVVNASILPLLFISNVFIRIEEPPAWMDLLAGIFPVRPFADAMLATYSPR
                     LAPGGLEWQDLAVIAGWGIAAVVFSVRFFSWEPRR"
     gene            complement(5753..6679)
                     /locus_tag="VHK28_02385"
     CDS             complement(5753..6679)
                     /locus_tag="VHK28_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141111.1"
                     /translation="MTAPEPAIELRGLAKRYGQIEAVRGIDLRVERGEVFALLGPNGA
                     GKTTTVEILEGHRRRDAGEARVLGHDPGRGERRLRERIGIVLQSTGVEEYLTVHEVVA
                     LHAGYYPRPRPVPEVLELVGLAEQADRPVRKLSGGQRRRVDLAVALAGDPELLFLDEP
                     TTGFDPGARRQAWEVLRGLTALGKTIFLTTHYMDEAQAVANRVAVIAGGRIVAEGPPS
                     TIGGRQGARSRVRFQLTDGTELPDRFGARRDGPAQTPTWELETVRVTRLLHELTEWAL
                     SADVELTGLEVRQPTLEDVYLELVGESSREAP"
     gene            complement(6694..6900)
                     /locus_tag="VHK28_02390"
     CDS             complement(6694..6900)
                     /locus_tag="VHK28_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141112.1"
                     /translation="MTQNLPVPRDEAPLVVDTVHDLHPDHHDPQHQEVRQNVAVALLP
                     LALITLLTLAFVAAAWTFLAAQGA"
     gene            complement(6914..7471)
                     /locus_tag="VHK28_02395"
     CDS             complement(6914..7471)
                     /locus_tag="VHK28_02395"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2141113.1"
                     /translation="MTAASAARRPLGTGLALRWRIVGAVLPLLLLAAWGTMLALRPGP
                     AGARIGEPAPDFALVDLNGTPIRLADLRGRPVIVNFWASWCGPCVEEFPILESASRLH
                     GPHGLTVLGIVFRDNSEAARVFMDRMGASWSALMDPGEEVARRYGIYGPPETFFIDPE
                     GTVVARQIGQLDRAALDRHLAELLR"
     gene            complement(7479..7868)
                     /locus_tag="VHK28_02400"
     CDS             complement(7479..7868)
                     /locus_tag="VHK28_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141114.1"
                     /translation="MGESAVGFLILGVGILAILALVFAVTLRSGRAAAGRPEPPRGVH
                     LPPPSYLPVVMSVGAALIGAGLSFRPEEPMTMPVMDVVSGLMHPLIGPLGVIVLLYGV
                     WAWVRAAGHEWRETERGAHGHEDARGH"
     gene            complement(7881..8504)
                     /locus_tag="VHK28_02405"
     CDS             complement(7881..8504)
                     /locus_tag="VHK28_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX2141115.1"
                     /translation="MSVEAKTSMSADITAHPELSRRETGMPTPLVGMLLFIASEVMFF
                     GGLFAAYFNARATFPGEWRPPAPAHELELLPLALPLTVILVISSFTMQFGVAAIRRGD
                     QGRLRMWTAITLLLGIAFLAGQLYDYSILGFGISDGVFGTVFYTLTGFHGAHVFGGTV
                     GLTILLARAMQGQFSARNHVAVEAVSMYWHFVDVVWIALFTTLYILK"
     gene            complement(8501..>8981)
                     /locus_tag="VHK28_02410"
     CDS             complement(8501..>8981)
                     /locus_tag="VHK28_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2141116.1"
                     /translation="LHYVFFGGSVFGVFAATYFWFPKMTGRRLNEGLGKVHFWIHFIG
                     FNLAFFPMHPLGISGMPRRIADYAADPIWMFWNFVSTIGAFMIAISVLPFLVNVVATL
                     INGEQVGDDPWEANTLEWATTSPPPAYNFDTLPPIRSERPVFDLRHKHDAEHLGDHA"
BASE COUNT         1432 a         3106 c         3205 g         1238 t
ORIGIN      
        1 ggtggcggtc gccgcctcca cctcgcccac cgactccagc cagcgctcgg cgtcgatggc
       61 cgccttgcag ccctcaccgg cggcggtgac cgcctggcgg tacttgtggt cgtggacgtc
      121 gcctgccacg aacacgccct cgacgttgct ggccgtgtcg ccgtcgcggg ccgtcttgca
      181 gtagccgcgc gggtcgagct caagctggcc ggcgaagagc tcagtgttcg gcttgtagcc
      241 gatggccacg aacagcccct ggacgttgag cgtcgactct tcgccgctga ccgtgtcacg
      301 cagcgcgagc tcggtgaccg tcatgtcgcc gcgcacatcg gtcacctcgg tgttccagcg
      361 cacctcgatc ttgggatggc tcagcgctcg gtcctgcatg atcttgctgc cccggaactg
      421 gtcacggcgc accagcatgg tgatcttgga cgcgaagcgg gtcaggaaca gcgcctcctc
      481 cagagcggtg tcgccgccgc cgaccacggc gatctcgcgg tcgcggaaga agaagccgtc
      541 gcaggtggcg caggcgctca cgccacggcc gcggaaggcc tcctccgagg ccaggcccag
      601 ccacagcgcg gacgcgccgg tggcgacgat gaccgagtcg gccagccact gctcgtcgcc
      661 gacccacagc ttgaacggtc gctcgctgaa gtcgacccgg gtgacgtcct tctcgaccat
      721 ggtggtgccg aatcgttcgg cctgctcgcg gaactgctgc atcagctccg gcccctggat
      781 cccctgcggg aagccggggt agttctcgac gtcggaggtg atcatgagct gtccaagcgg
      841 cacgttgccg gcgaagacga tcggctccag ccgtgcgcgc gaggcgtaga tggcggcggt
      901 caggccggcc gggccggaac cgatgatcgc gaccttgcgg tggcgggtgt cggtctgttc
      961 tggggtttcg gtgcccgtcg tgggcggaac ggtcgcggtc atgaggtgag catagcgaac
     1021 ctgtcgaacg gaccgatgcg caccaggccc cacgcgcgca gcagctccgg caggttgacc
     1081 gccaggtccc aggcgccgac ggccaccagc gccagcccga ccagcacccc gatggtgcgc
     1141 tcgtggcgca ccatggcgcg cgccaatcga gctccccgcc actgcccgat gatgccgatc
     1201 acgaacagtg gcaccccgaa gcccagcccg taggcggcga agaaggcgag ctggcccagc
     1261 gcgtcgccga tggtcaggct gaaggcgaag atgcccagca ggaacgggcc gatgcaggga
     1321 atggcgatcg gcgcgaacag cagcccgtac aggtaggcgc cggcggccgc tcccccggcg
     1381 cggggcgtgg ccgagggctg cggcaggcgg gcgaaggggt tgacccccgc cagcagcagc
     1441 acgcccaggg tgatgaggag caggtcggcg gcgggcagga tgaccgggat catgcgcgaa
     1501 agcggcgctg acagggcgat gaccaccgcg gcgatgacca gcatgccgct catcacgccg
     1561 gccaggacga gggcggccgc cagcattggg gaaaggcggc gcacggggct gccggccacg
     1621 gcgacaccgc cgccatcggg gagcaccacg ttgcccgtcg cggccccgcc ggtcaggtag
     1681 gcgatgaagg ccgggtagag cggcaggatg cagggagcgg cggttgaggc cacgccggcc
     1741 gcgaaggccg ccagcagctc gcccatgtca gcccgcggcg atctcggcca ggagcgcttc
     1801 cacgtcgtag ccgccgaagt cgagggcgcg aatcgaaccg tccgggaaga ggagcgccat
     1861 gggggtattg ggcgggaagc ggaagccgtt gccgaaccga tcggtcagcg cggtggcgat
     1921 ctccgggttg gccatggcga agggccactc gaagccctgc tcctcgacat aggccgccag
     1981 gtcggccggc agctcgctgg ggtcgacatc gatgccaacc gaatggaagt cggctcgctc
     2041 atgtgcctcg atgaccctat gcatctgcgc ccggcagttc gtgcaccaga tggccatggc
     2101 ctccagcagc accggcttct cggccgccag ctcgccgagc gtgaactcgg tcccgtcccg
     2161 cacgtcggtc aggaccaggg tgtgaagcgg gtcgctgccc agggcgggca tctggctggc
     2221 cgagggggcg tcgctggcaa ccgccgtccc cgacgcgggt ttgtcgctcg cggtgccgcc
     2281 atcggtggcc gttgcctccc gcgccgaggc ggagggagtg gtggcggagc aggccgtccc
     2341 caggacgagg acggcgagca gggcgatcgg cacgggccgg cgaccagcag gcatccggcg
     2401 acgatagcgc cgaattgtga cgaccgggtg tcgggcgtgc gaagacgccc tgctacggcc
     2461 gtgggctagg ggcgtggagt gcgatcggcc aaaccgggct cgtccaggtc cttgggcgcc
     2521 ggctcgtcag ggtcgtcgcg accctggccg gcgtcgtgcg gccgctcgat gtcatccatg
     2581 gggtgcgtat caggcgtgag ctcgtccggc tcgccgggat gcggctcgcg cgggacgcgg
     2641 tagatgtcat ccatggtcag gcctccactc cgttgctcgc caatccactg accgcccagc
     2701 gcagggcaac ctcgaacgcc ccggcgccgt cggccttgtc caccacccgg gtgtgcgggt
     2761 cgtcgcgcac catgtcgggc aggtgatcgc agggcgtcgc cgtccagcac accaccggca
     2821 gctcagggaa gcggtgccgg atcgccatga aggcggcgga ccccacctcc cgttcgagcc
     2881 gccaatcgag gaccaccgcc gccggcgcgc ccgcctcgca gtcgcgcagg aacgcgccgg
     2941 gcgcggcata gatggcggcg gtctgtccca tgcccacgca catctccctg gccagctcgg
     3001 ccacggcggg gtcatcttcg accacgcaca ccgccgcctc gccgggagcc agagagcgcg
     3061 tcgccggatt gggcgcaagg gacaggttca ggcccacacg atcgggtcct tgcaggctgc
     3121 gtgcccatga aggtcctgga tcgaagatcg cggtgcgcgg cggcaaccgg tacgatcttc
     3181 gacgtgatca ccaggcgcac caccacctcg gtcgacgtgg gcggcgtcag catcggctcc
     3241 gcccacccga tcgtcgtgca gtccatgaca aataccgata ccgccgatgc ggacgcgacc
     3301 gccatccagg tcgcgcagct tgcgcatgcc ggcagcgagc tggtacgggt cacggtcaac
     3361 aacgacgagg ccgcccaggc ggtcccgcgc atccgcgaga agctcgaccg cctcggcgtc
     3421 ggcacacccc tgatcggcga cttccactac aacggccacc tgctgctgag caggtacccg
     3481 gaggcggccg ccgcgctggc gaagtaccgc atcaacccgg gcaacgtggg cgccaagcga
     3541 cgcgatgcga acttccagca gatcgtggag gtggccgtcg ccaacgcgaa gccggtgcgc
     3601 atcggagtga actggggcag cctcgaccag cagctgctga ccgagatgat ggatgccaac
     3661 gccgcccttc ccgagccggc cgacagccgg gaggtgatga tcgaggccat gatcgagtcg
     3721 gcgctgcgct cggcgcggct ggcggaggag accggtctgg cccacgaccg catcatcatc
     3781 tcggccaagg tcagcggcgt gcaggacctg gtggaggtct acggccgcct ggcaaagcgc
     3841 tgcgactacc cgctgcattt ggggctgacc gaggccggca tgggatcgcg cggcatcatc
     3901 acctccaccg ccggcctggc catcctgctg cagcgcggca tcggcgacac catccgcgtg
     3961 tcactcacac cctcgcccgg cggcgaccgg accgaggagg tgcaggtcgc ccagcaggtc
     4021 ctccaggcgc tcggcctgcg cagcttcacg ccgcaggtca gtgcctgccc gggctgcggc
     4081 cgtaccacct cgaccttctt ccaggagatg gcccgcgaca tcaccgccta cctggcggag
     4141 aagatgccaa tctggcgcga gcgtcatccg ggcgtggagg agctcaaggt ggcggtcatg
     4201 ggctgcgtgg tgaacgggcc cggcgagtcc aagcacgcgg acatcgggat cagcctgccg
     4261 ggaacgttcg aggagccggt cgcccccgtc ttcgtggacg gacagctgca caccacgctc
     4321 cgcggcgacg acatcgtgcc gcgcttcctg gagatcctgg acgggtacgt cgagcgccgc
     4381 tacccgacct agaccaatgc ctgccggcgg cgtggcgccg gcaggttcag cccgatccgg
     4441 ttggtcagtt gcagatgccg gcctgggccg gggctccgat caggcgaccg ggggccaggc
     4501 tctcctgcgt caccgagccg tcctcgttga cccagacctc ggcgatgagg ttgcggtcca
     4561 gctggttgtc ctggagctgg gtggagtcga tggccgtctg ccccgccacg gtgagcaccc
     4621 agtcctcgaa ggccgggaag tcgagctcga ggacctgcgc ggagcaggtg gcgatggccg
     4681 tctcctcgcc gggggtgccg gccacgtcca gtgagaccgc ggcgtcgcct tccgtctcgt
     4741 tgaccaccgc aacacggagg gtcaaggtgt caccgtcgcc tccgccaccg ccaccgccgc
     4801 cgcctccgca ggcggcgagc gcgaggccga gcaccagcag catcgcggtg agtccgtggc
     4861 gcggtcgagc ggcggacggg attgggcgtt ccgtcatcga aatcgatcct ccttcatgct
     4921 ccctccgagg gtacccgcgg tgtgggcgcg ctgtcccgcg gactctacgc cctagccgat
     4981 gtcatacgcc agcctcagcg cctcggctcc cagctgaaga agcgcacgct gaagacgacc
     5041 gcagcgatgc cccagcccgc gataaccgcc aggtcctgcc actccaggcc gcctggcgcc
     5101 agccgcgggc tgtacgtggc cagcatggca tcggcgaagg ggcgcaccgg gaagatgccg
     5161 gccagcaggt ccatccaggc cggcggctcc tcgatgcgga tgaagacgtt gctgatgaac
     5221 agcaggggca ggatgctggc attcacgacc gccggggcgg cgtccgcgtt cgggatcagg
     5281 gcgctgaccg ccaggcccag ggcgcagaag ctggcggcgg cgatcaccag ggtcagcagc
     5341 agctgcggcg accggtccca gggaaccgcc acgtcgtaga gcagggcgcc gaaggcggcg
     5401 acgatgacca ccagcagcag cgcgatcagc accgcatggg cgatgcgccc gaagatgtag
     5461 gcccaggccg ggagaggggt gccgcgcacg cgcttcagca cgccgttgtc gcgggcgatg
     5521 gccacggtca tggcgatgtt ggtgtaggtg gcggtgataa cggcgaagac gatgatggcc
     5581 ggcgtgaaga agctggaggc cgagacgtcc ccggtgtcaa tgccggcgaa gatgacgttg
     5641 aagatgacca tgaacagcag cgggaaggcg aaggtgaaga aggcggccgc cggattgcgc
     5701 cagaacgccc ggttctcgta gcggacctgg cggaccgcca gcgcgaggtc gctcacggcg
     5761 cctcccggct gctctctccg acgagctcca ggtacacgtc ctcgagcgtc ggttgccgca
     5821 cctcgaggcc ggtgagctca acatccgcgg agagcgccca ctccgtcagc tcgtggagca
     5881 gccgggtgac gcggaccgtt tcgagctccc aggtgggcgt ctgggcgggg ccgtcgcgcc
     5941 tggcaccgaa ccggtcgggc agctcggtgc cgtccgtcag ctggaaccgc acccggctgc
     6001 gggctccctg gcgtcccccg atggtggatg gcggcccctc ggccacgatc cgcccgccgg
     6061 caatgaccgc cacgcggttt gccaccgcct gtgcctcgtc catgtagtgg gtggtcagga
     6121 agatggtctt gcccaacgcg gtcaggccgc gcagcacctc ccacgcctgg cggcgggcgc
     6181 ccgggtcgaa tccggtcgtc ggctcgtcca ggaagagcag ctcagggtcg ccggccagcg
     6241 ccacggccag gtccacccga cgccgctgtc cgccggacag cttccgcacg ggccggtctg
     6301 cctgctcggc gagcccgacc agctcgagca cctcgggcac cgggcgtggg cgtgggtagt
     6361 aaccggcgtg cagcgcgacc acctcgtgca ctgtgaggta ctcctccacc ccggtcgact
     6421 gcagcacgat cccgatccgc tcccgtagcc ggcgctcccc gcgaccgggg tcgtgaccca
     6481 gcacccgagc ctcgccggcg tcccgccgac gatgtccctc caggatctcg acggtggtcg
     6541 tcttccccgc cccgttgggc cccagcagcg cgaagacctc gccgcgctcc acgcgcaggt
     6601 cgatgccgcg caccgcctcg atctggccgt accgcttggc caggcctcgc agctcgatcg
     6661 cgggctcggg tgccgtcacc gcgtcggtcg cggtcaggcg ccctgggcag ccaggaaggt
     6721 ccaggccgcg gcgacaaagg ccagcgtcag gagggtgatg agtgcgagcg gcagcagcgc
     6781 cacggccacg ttctgacgca cctcctggtg ctgcgggtca tggtggtcgg gatgcaggtc
     6841 gtggacggtg tcgaccacga gcggtgcttc atcgcggggt acgggcaggt tctgggtcat
     6901 ggggcgtgat ctcctaacgc agcagctcgg ccaggtggcg gtccagggcg gcccggtcga
     6961 gctggccgat ctggcgtgcg accaccgtgc cctccgggtc gatgaagaat gtctcgggcg
     7021 gcccgtagat cccgtagcgg cgggccacct cctcgccggg atccatcagg gcgctccagc
     7081 tcgcgcccat gcggtccatg aaaacgcgag cggcctccga gttgtcgcgg aagacgatgc
     7141 ccagcacggt caacccgtgc ggcccgtgga ggcggctggc ggactcgagg atcgggaact
     7201 cctccacgca cgggccgcac cagctcgccc agaagttgac gatcaccggg cgtccccgca
     7261 ggtcagcgag tcgtatcggc gtgccgttga ggtcgaccag ggcgaagtcg ggtgccggct
     7321 cgccaatgcg cgctccggct gggccgggac gcagcgccag catcgtgccc caggccgcca
     7381 ggaggagcag cggcaggact gctcccacga tccgccagcg gagcgcgagc ccggtgccca
     7441 ggggccgtcg cgcggccgag gcggcggtca tggcgcgctc agtgcccgcg ggcgtcttcg
     7501 tggccatggg cgccacgctc cgtctcgcgc cactcgtggc cggcggcacg cacccaggcc
     7561 cagacgccgt agagcaggac gatcactccc agcgggccga tcagcgggtg catcaagccg
     7621 gacaccacgt ccatgacagg catcgtcatc ggctcctcgg gcctgaagct cagcccggcg
     7681 ccgatgaggg ccgccccgac cgacatcacg accggcaggt agctgggcgg cggcaggtgg
     7741 accccccgag gcggctccgg ccgcccggcg gcagcccggc cggagcgcag cgtgacggcg
     7801 aagaccagcg ccaggatggc caggatgccg acgcccagga tcaggaagcc gactgcgctc
     7861 tcgcccacct atcggtcctc ctacttcagg atgtagagcg tcgtgaacag ggcgatccag
     7921 accacgtcca cgaagtgcca gtacatgctg accgcctcca ccgccacgtg gttgcgggcg
     7981 ctgaactggc cctgcatggc gcgcgcgagc aggatggtga gtcccaccgt gccgccgaag
     8041 acgtgcgctc cgtggaaccc ggtcagggtg tagaacacgg tgccaaacac gccgtcgctg
     8101 atcccgaagc ccaggattga gtagtcgtag agctgaccgg ccaggaaggc gatgcccagc
     8161 agcagggtga tggcggtcca catccgcaac cgaccctggt ccccgcgccg gatggcggcc
     8221 acgccgaact gcatcgtgaa gcttgagatg accaggatca cggtcaacgg cagcgccagc
     8281 ggcagcagct ccagctcgtg cgccggggcc ggcggacgcc actcccccgg gaaggtggcg
     8341 cgggcgttga agtaggcggc gaacaggccc ccgaagaaca tgacctcgct ggcgatgaag
     8401 agcagcatcc ccaccagcgg ggtgggcatg cccgtctcgc ggcgagacag ctcggggtgc
     8461 gcggtgatgt ccgcgctcat gcttgtcttg gcctcgacgc tcatgcgtgg tctcccaggt
     8521 gctcggcatc gtgcttgtgc cgcaggtcga agaccgggcg ctcgctgcgg atgggcggca
     8581 gcgtgtcgaa gttgtaggcc ggcggcggag aggtggtcgc ccactccagg gtgttcgcct
     8641 cccacgggtc gtcacccacc tgctcgccgt tgataagggt ggccaccacg ttcaccagga
     8701 agggcagcac gctgatggcg atcatgaagg cgccgatggt ggagacgaag ttccagaaca
     8761 tccagatcgg gtccgcggcg tagtccgcga tgcgccgcgg catgccgctg atgcccagcg
     8821 ggtgcatcgg gaagaaggcc aggttgaacc cgatgaagtg gatccagaaa tggaccttgc
     8881 ccaacccctc gttcagccgg cggccggtca tcttggggaa ccagaagtag gtcgccgcga
     8941 agacgccgaa cacgctgccc ccgaagaaca cgtagtgcag g
//
LOCUS       DATACA010000114         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2185137, whole genome shotgun sequence.
ACCESSION   DATACA010000114 DATACA010000000
VERSION     DATACA010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185137"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VHK28_02415"
     CDS             complement(<1..690)
                     /locus_tag="VHK28_02415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2141117.1"
                     /translation="MAFRNRPVLDRKHRPRWQDELRSQQVMVGGFALAIAVAIGIFAA
                     AGWSSYYDSSLRQVALVGGDPVQRGEVNRRSDIIGAELTATFLDLNSQLGGSRDQIIQ
                     QQISTLDSTLTNLPSVASDAVVTGRTLQQQADELGISVDPASVDAEVAERRNLPERRK
                     LSLIMVFPEKDEGAPAESEPTEQDWAEARAEAEAILAEIEAGGDFAALAAERSDDQTK
                     ASNGLLGWIQAD"
     gene            complement(782..1546)
                     /locus_tag="VHK28_02420"
     CDS             complement(782..1546)
                     /locus_tag="VHK28_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX2141118.1"
                     /translation="MTAKPRVGLAAGRGVCVVLPTYNERENLEPMAAAILTSLPEARL
                     LVVDDASPDGTGMLADTLAATEPRVSVLHREGKEGLGVAYRHGFRWALDQPDVRAVVQ
                     MDADMSHDPADLPRLLEPLMRDADLVLGTRYMRGGGTVGWPMRRQLISRAGTLFARTV
                     LLLPYRDLTGGFKAWRRELLDAIRLRDANASGYGFQIETTWWAHRRGARIVQVPIIFR
                     ERVAGYSKMSRSIVREALLVVLRLRGEAIVDAVRRR"
     gene            complement(1553..>2164)
                     /locus_tag="VHK28_02425"
     CDS             complement(1553..>2164)
                     /locus_tag="VHK28_02425"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX2141119.1"
                     /translation="IALANEYARYAAQRGIDVLEVIEAANSQPYSHIHQPGVGVGGHC
                     IPVYPHFLFNTDASLELPPLARRINEGMGRWTVDLVRGRIGSLQQAAVLVLGIAYRAD
                     VQEDAFSSAFRLRDELLAAGARVYGHDPYFDAAHLRELGFEPWDPDDPQPVRVAVLQA
                     GHRQYRSMDLSRIPGLELVVDGRNVLDRAEVEGAGLAYAGIGR"
BASE COUNT          296 a          764 c          778 g          326 t
ORIGIN      
        1 gtcggcctgg atccagccca gcaggccgtt gctggccttg gtctggtcgt cgctgcgctc
       61 ggcggccagc gccgcgaagt cgccgccggc ctcgatctca gccaggatcg cctccgcctc
      121 cgcgcgggcc tccgcccagt cctgctcggt gggctcgctc tcggcggggg cgccctcgtc
      181 cttctccggg aagaccatga tcagtgacag cttgcggcgc tcgggcaggt tgcggcgctc
      241 ggcgacctcg gcgtccaccg atgccggatc aacggagatg cccagctcgt ccgcctgctg
      301 ctggagggtc cgaccggtga ccaccgcgtc ggaggcgacc gatggcaggt tggtgagcgt
      361 gctatccagg gtgcttatct gctgctggat gatctggtcg cgcgacccgc ccagctggct
      421 gttcaggtcc aggaaggtgg ccgtcagctc ggcaccgatg atgtcgctgc ggcggttcac
      481 ctccccgcgc tgcaccgggt cgccgccgac cagcgccacc tggcgcaggc tactgtcgta
      541 gtacgagctc catccggcgg cggcgaagat gccgatggcc acggcaatgg ccagcgcgaa
      601 gccgccaacc atcacctgct ggctgcggag ctcgtcctgc cagcgtgggc ggtgctttcg
      661 atcgaggacg ggtcggttgc ggaaggccat gctcgggtgc tcgtgcgcag gggttatcag
      721 cggggttgcg ttctgggcga gggagaatgc tagccgatgc cctccagcgg cgccaccgcg
      781 ctcaccggcg gcggacggcg tccacgatcg cttcgccgcg caggcgcagg acgaccagca
      841 gggcctcgcg gacgatgctc cgcgacatct tcgagtaccc ggccacgcgc tcgcggaaga
      901 tgatcggcac ctggacgatg cgcgcgccgc ggcggtgggc ccaccaggtg gtctcgatct
      961 ggaagccgta gccgctggcg ttcgcgtccc gcagccggat ggcgtccagc agctcgcggc
     1021 gccaggcctt gaagccgccg gtcaggtcgc ggtacggcag gagcagcacg gtgcgggcga
     1081 acagggtgcc ggctcggctg atgagctggc gcctcatcgg ccagcccacc gtcccgcctc
     1141 cgcgcatgta gcgtgtgccg agcacgaggt ccgcgtcgcg catcagcggc tcgagcaggc
     1201 gcggcaggtc ggccggatcg tggctcatgt cggcgtccat ctggaccacg gcccgcacgt
     1261 ccggctggtc gagcgcccag cggaagccgt gacggtaggc gactcccagc ccctccttgc
     1321 cctcccggtg caggaccgat acccgtggct cggtggccgc cagcgtgtcg gccagcatcc
     1381 cggtgccgtc cggcgatgcg tcgtcgacca ccagcagccg tgcctcgggc agcgacgtca
     1441 ggatggcggc tgccatcggc tccaggttct cccgctcgtt gtaggtgggc agcaccacgc
     1501 acacgccgcg accggcggca agcccgaccc gtggcttggc cgtcatggcc ggctagcgcc
     1561 cgatgccggc gtaggccagg ccggcgccct caacctccgc gcggtcgagg acgttgcgac
     1621 cgtcgaccac cagctcgagc ccggggatgc gcgagaggtc catggaccgg tactggcggt
     1681 gcccggcctg cagcaccgcc acccggaccg gctgcgggtc gtccgggtcc cacggctcga
     1741 agccgagctc gcgcaggtgc gccgcatcga agtacggatc gtggccatag acgcgggcgc
     1801 cggccgccag cagctcgtcg cggagccgga aggcggagct gaaggcgtcc tcctgcacgt
     1861 cggcacggta ggcgattcct agaaccagca ccgctgcctg ctgaagagaa ccgatgcgtc
     1921 cgcgcaccag gtcgaccgtc cagcggccca tcccctcgtt gatgcgccgg gccagcggcg
     1981 gcagctcgag gctcgcatcg gtgttgaaca ggaagtgcgg gtacaccggg atgcagtgcc
     2041 cgccgacgcc gacgcccggc tggtggatgt ggctgtaggg ctgcgaattg gcggcctcga
     2101 tgacctccag cacgtcgatt ccacgctgtg cggcgtaccg ggcgtactcg ttggccagcg
     2161 cgat
//
LOCUS       DATACA010000115         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1622600, whole genome shotgun sequence.
ACCESSION   DATACA010000115 DATACA010000000
VERSION     DATACA010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622600"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VHK28_02430"
     CDS             <1..1648
                     /locus_tag="VHK28_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141120.1"
                     /translation="KACAGRLAGRVILPSQRPREIRLTITAHDVEPVPDAEGQAARRR
                     AADRWREAATRVHVPGHRLVERMLGQASSDLVSFALLEGSRDEWLAPSAGMPLYPALF
                     GRDSLTTCWQAALLDRGRLLDASMTRLRRLQGVRDDPARDEQPGRIIHSLRRGRLART
                     GANPFAQYFGDFASPLMFVISLAHLYAWTGDKADLERHWDAARRALDWACDAGDRDGD
                     GYLEYRTLAEQGPKNQGWKDSGEAIVYQDGRPVPAPIATCELQGYWFAAQQLGAVLAA
                     VQGRLNDARDYWRSAVDLKRRFNRDWWMAEEGFIALALDPDKRQVRSVGSNAGHCLTA
                     GIISRDHIPSVVGRLFSPDMFSGWGIRTLASTHPAYNPIGYHLGSIWPVENATIGFGL
                     RRYGFDIRFLDLMQAMLDLASLYQGGRVPECVGGYPRSMFPHPGAYPQANTPQAWNQS
                     ALPLLLHSLLGLQPVAALDLLVVDPVLPRWLPEVTLERLRVGGATATLRFYRDRGGES
                     HVEVVRKRGTLHVLRQPPPESLTVGAKDRFTALVDRLMHH"
     gene            1687..>2634
                     /locus_tag="VHK28_02435"
     CDS             1687..>2634
                     /locus_tag="VHK28_02435"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2141121.1"
                     /translation="MAPTLKKVEDQVVVITGASSGIGLVTAKMAAARGARVLLAARNE
                     RDLQSAVDEIRRAGGRALHAAADVARPEDVARVADMAVREYGRIDTWVNNAAVAVYGR
                     LMDLAVPDMRRQFDINYWGQVYGSRAAVPHLARQGGALINVASALADRAIPLQGNYCA
                     AKHALKAFTDSLRMELEDEGAPISVTLIKPGSIDTPLFEKARTLLDVEPQPVPPVYAP
                     ELVARAILHCAEHPVRDLVVSGMGKVLSVAGTVAPRLTDRYMERSTFEAQMSDLPLAD
                     ARPDNLYDPVEHDGGERGRNWTGRGHARSLQTWAALHPAG"
BASE COUNT          405 a          897 c          926 g          406 t
ORIGIN      
        1 gaaggcctgc gccgggaggt tggccggtcg tgtgatcctg ccgtcccagc gtccccggga
       61 gatccggctc accatcaccg cgcacgatgt cgagccggtg ccggatgccg aggggcaggc
      121 ggcgcgcagg cgggctgcgg atcggtggcg ggaggccgcc acccgcgttc acgtgccggg
      181 ccatcgcctg gtcgagcgga tgctgggcca ggcaagcagc gatctggtgt cgttcgccct
      241 gctcgagggc agccgcgacg agtggctggc tccatcggcc gggatgccac tgtacccggc
      301 gctcttcggc cgtgactccc tgaccacgtg ctggcaggcc gcgctcctcg accggggccg
      361 cctgctggac gcgtccatga cgcggctgcg acggctccag ggtgtacggg acgaccctgc
      421 gcgggacgag cagccgggac ggatcattca ctcgctccgg cgcgggcggc tggcccggac
      481 cggagcgaat ccctttgcgc agtacttcgg cgacttcgcc agtccgctaa tgttcgtgat
      541 cagcctggcg cacctctacg cgtggaccgg cgacaaggcc gacctcgagc gacactggga
      601 tgccgcccgt cgtgccctgg actgggcgtg tgacgccggc gatcgggacg gcgacggata
      661 tctggagtac cggacgctgg cggagcaggg gcccaagaat cagggctgga aggacagcgg
      721 agaagcaatc gtgtaccagg acgggcgccc ggtgcccgcg ccgatcgcca cctgcgagct
      781 ccagggctac tggttcgccg cccaacagct cggcgcggtg ctcgccgccg tccagggtcg
      841 tttgaacgat gccagggact actggcgatc agcggtggat ctgaaacggc ggttcaaccg
      901 ggactggtgg atggccgagg aaggcttcat cgcgctcgcg ctggacccgg acaagcgcca
      961 ggtccggagc gtcggctcga acgccggaca ctgcctcacg gcgggcatca tctcgcgaga
     1021 ccacattccc tccgtcgtgg gacgcctctt ctcgcccgac atgttcagcg ggtggggcat
     1081 tcgtaccctg gcgagcacgc atcccgccta caacccgatc ggctatcacc tcggcagcat
     1141 ctggccggtg gagaacgcga ccatcggctt cgggctccgc cgctacggct tcgatatccg
     1201 ctttctcgac ctgatgcagg ccatgctcga cctggcgagc ctctatcagg gcgggcgcgt
     1261 cccggagtgc gtcggcgggt atcctcgctc gatgttcccc cacccgggtg cctaccctca
     1321 ggccaacaca ccgcaggcct ggaatcagag cgccttgccg ctcctcctcc acagcctgct
     1381 cggtctgcag ccggtcgccg ccttggacct gctcgtagtg gatccggtct tgccgcggtg
     1441 gctgccggaa gtgacgctcg agcgactccg tgtcggtgga gccaccgcga cgcttcggtt
     1501 ctaccgtgac cgcggaggcg agtcgcacgt agaggtcgtt cgaaagcggg gaacgctgca
     1561 cgtcctgagg cagccgcccc ccgagtcgct gacggtgggc gcgaaggacc gtttcacggc
     1621 cctggtggac cgcctgatgc atcactgagc ggtacgtcac gtggatcaga ggaatacgag
     1681 ggactcatgg ctcccacgct gaagaaggtc gaagaccagg tcgtggtgat caccggtgcc
     1741 tccagcggca tcggactggt gacggcaaag atggccgcgg cacgcggggc tcgggtgctc
     1801 ctggccgcgc gcaacgagcg cgatctccag agcgccgtcg acgagatccg ccgggccggc
     1861 ggtcgggccc tccatgcggc ggccgatgtg gcccgtcccg aggacgtcgc gcgcgtggcc
     1921 gacatggccg tgcgggagta cggccgcatc gacacctggg tgaacaacgc ggccgtcgcg
     1981 gtatacggca ggctcatgga tctggccgtg cccgacatgc gccgccagtt cgacatcaac
     2041 tactggggcc aggtctacgg ctcacgggct gcggttcccc atctcgcgcg tcagggcggg
     2101 gcgctcatca acgtggccag cgccttggcg gaccgtgcca tccccctcca ggggaactac
     2161 tgcgcggcca agcacgccct gaaggccttc accgactccc tccgcatgga gctcgaggac
     2221 gaaggcgcac cgatctcggt cacgctgatc aagcccggca gcatcgacac gccgctcttc
     2281 gagaaggccc ggaccctcct ggatgtggag ccgcagcccg tgccgccggt ctacgccccc
     2341 gagctggtcg cgcgcgcgat actccattgc gccgagcacc cggtccggga tctggtggtg
     2401 agcgggatgg gcaaggtcct gagcgtcgcg ggcaccgtcg ctccgaggct gaccgaccgc
     2461 tatatggagc ggagcacctt cgaggcccag atgtccgatc tgccgctggc ggacgcccgg
     2521 ccggacaacc tctatgaccc ggtggaacac gatggtggtg aacgggggcg caactggacg
     2581 gggaggggcc atgcgaggag cttgcagaca tgggccgcgc ttcatccggc gggc
//
LOCUS       DATACA010000116         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1141162, whole genome shotgun sequence.
ACCESSION   DATACA010000116 DATACA010000000
VERSION     DATACA010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141162"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1504
                     /locus_tag="VHK28_02440"
     CDS             167..1504
                     /locus_tag="VHK28_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEX2141122.1"
                     /translation="MTDLLTSANAWLNGLVWGPPMLVLLVGTGVLLTIVTGGVQFRRL
                     GTALGEVLGKLRRPGGGEGSVTPFQAVATALASTVGVGNIAGVATAIAVGGPGALFWL
                     WISGIFGMATKFSEIVVALHYRERDAAGTMRGGAMYTLRKLGYPRLGATFALLTALAA
                     FGIGNMVQANSVAQSLESTFGASPPVVGFVLVLLAGAVILGGIRRIAAFAEVLVPFMA
                     LVYIGGGLVVLALHAGAIPAAIRLVFDGAFSGTAATGGFAGSTIMLAMRYGIARGLFS
                     NEAGLGSAPLVHAAANTDHPVRQGLYGIFEVFVDTLLICTTTGLAILVTGAWEGGETG
                     AALSGKAFETGLPGVWGDILVTSGLVLFAFSTLIGWSYYGETGIVYLLGSRAALPYRL
                     AWLVFIYLGAVGSLQVIWSVADTLNGLMALPNLLSVLLSIPLLRRLQREFFER"
     gene            1501..2313
                     /locus_tag="VHK28_02445"
     CDS             1501..2313
                     /locus_tag="VHK28_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEX2141123.1"
                     /translation="MTPRPGELRARLHEIIFEADTPEGRLFDLLLLVAIVTSVIVVLL
                     ESVASVRARVGPTLRAMEWGFTVLFTVEYLLRLFTVGRPLRYARSAFGLIDLLAILPT
                     YLSLLLPGAQSLLVIRLLRLLRVFRVLKLAEYLRESRTLVLALRASARKIFVFLLAVM
                     TIVVVVGTLMYVIEGERHGFTNIPISIYWAVVTLTTVGYGDLAPATTLGRGLAALLML
                     TGYGIIAVPTGIVTAELTRATMAPVSTQACPACGVGGHEHDAAYCRRCGSRL"
BASE COUNT          292 a          840 c          857 g          411 t
ORIGIN      
        1 cggcggtgcg ctcggcggtg cgcgggtgtg cgcgggcggg tgatgtacgc agggcggggc
       61 gcgcggtctg gcggagccct cacccggctc gaggagtcca ccacgcgcac tatcgccccg
      121 caaccggccg ccctatcata cgcggcaact ccgaccctca acgaccatga ccgatctgct
      181 cacgagcgca aacgcctggc tcaacggcct cgtgtggggc cctccgatgc tggtgctgct
      241 ggtgggcacc ggagtcctgc tcacgatcgt caccggtggg gtgcagttcc gccgcctcgg
      301 gacggcactc ggcgaggtgc tcggcaagct gcgccggccg ggcggcggtg agggcagcgt
      361 cacgcccttc caggcggtcg cgacggcgct ggcctccacg gtgggcgtcg gcaacatcgc
      421 cggtgtcgcg accgcgatcg cggtcggcgg gccgggcgcg ctcttctggc tgtggatctc
      481 cggcatcttc ggcatggcga ccaagttctc cgagatcgtg gtggcgctcc attaccggga
      541 gcgggatgcc gcgggcacca tgcgcggcgg ggcgatgtac acgctgcgga agctcggcta
      601 cccgcggctc ggcgcgacgt tcgcgctgct caccgcgctg gccgcgttcg gcatcggcaa
      661 catggtgcag gcgaactcgg tcgcccagag cctggagagc acgttcggcg cctcgccgcc
      721 ggtggtgggc ttcgtcctgg tgctgctggc cggcgcggtg atcctcggcg gcatccgccg
      781 gatcgccgcc ttcgccgagg tgctggtgcc gttcatggcc ctggtataca tcggcggggg
      841 actggtggtg ctcgcgctgc acgcgggggc catcccggcc gcgatccggt tggtcttcga
      901 cggcgcgttc agcggcacgg ccgccaccgg cggcttcgcg ggcagcacga tcatgctcgc
      961 gatgcgctac ggcatcgccc ggggcctctt ctccaacgag gccggtctcg ggagcgcgcc
     1021 gctggtgcac gccgcggcga acaccgacca cccggtgcgc cagggcctgt acgggatctt
     1081 cgaggtcttc gtggacacgc tcctcatctg caccacgacg gggctcgcga tcctggtgac
     1141 cggcgcgtgg gaagggggcg agaccggggc cgcactcagc ggcaaggcgt tcgagaccgg
     1201 gctgcccggc gtgtggggcg acatcctggt gacctccggt ctggtgctgt tcgccttctc
     1261 caccctcatc gggtggagct actacggcga gaccgggatc gtctacctcc tcggcagccg
     1321 cgccgcgctc ccgtaccgtc tggcgtggct ggtgttcatc tacctcggtg cggtgggctc
     1381 gctccaggtg atctggagcg tggccgacac gctcaacggc ctgatggcgc tcccgaacct
     1441 cctctcggtc ctgctctcca ttccgctgct ccggcggctg cagcgggagt tcttcgaacg
     1501 gtgaccccgc ggcccggcga gctccgcgcc cgcctgcacg agatcatctt cgaggccgac
     1561 acacccgaag gaaggctttt cgacctgctg ctgctggtcg cgatcgtgac cagcgtcatc
     1621 gtcgtgctgc tggagagcgt cgcctcggtc cgggcgcggg taggccccac gctgcgcgcg
     1681 atggagtggg gcttcaccgt cctgttcacg gtcgagtacc tgctccgcct gttcaccgtg
     1741 gggcggccgc tgcgctacgc gcggagcgcg ttcgggttga tcgacctgct cgcgatcctg
     1801 cccacctacc tcagcctgct cctgcccggc gcacagagcc tgctcgtcat ccggctgctg
     1861 cgcctgctcc gggtgttccg ggtactcaag ctcgccgagt acctgcggga atcgcggacg
     1921 ctggtcctgg cgctgcgcgc cagcgcgcgg aagatcttcg tctttctcct ggccgtgatg
     1981 accatcgtcg tcgtggtggg cacgctgatg tacgtgatcg agggcgagcg gcacggcttc
     2041 accaacattc cgatcagcat ctactgggcc gtggtgaccc tgacgacggt cggctacggc
     2101 gacctggcgc cggccacgac cctcgggcgc gggctggccg cgctgctcat gctcacgggc
     2161 tacgggatca tcgcggtgcc gaccggcatc gtgaccgcgg agctgacccg cgcgaccatg
     2221 gcgccggtca gcacccaggc gtgcccggcc tgcggggtgg gcgggcacga gcacgatgcg
     2281 gcgtactgcc gccgctgcgg gagccggctg tgacatgcac cccgggtacg ggagggtatt
     2341 tttcgagcac cgtacctcct cacgatcgac ccggagaggc agtcgtgtcc cgtaccatcg
//
LOCUS       DATACA010000117         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1382219, whole genome shotgun sequence.
ACCESSION   DATACA010000117 DATACA010000000
VERSION     DATACA010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382219"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VHK28_02450"
     CDS             <1..1219
                     /locus_tag="VHK28_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2141124.1"
                     /translation="INVNIVNLELSALVQRVALDMASGASQYQVIYADPYQVLAPYHK
                     GLADLREFIEGEGFPPLEGGIDDFIPTQMDAAGRFEREGPIYALPYDCPTMIWQYRAD
                     LFDKYGQQMADDLGFDPTPGSDRTWEEYRQIAQWFNDNVEEVPYGTGHQAKQHDSLMC
                     DFSNILWAYGGDYFPNGQQVGLMGVSEPGPCLLGSPESMEAAAFYNSLLEVAHPGSAT
                     WDWDGVGAALRAGEIAMCPNWHEFAASNEEALPGKVHYAPLPKGPARAAHMYGGTGIS
                     VAGNATGAERGAAWLFVNWATAPQTQLSNLRSSAGGGTPTRTSVYQLPAVRAAEERPS
                     EMPNMLTADAVSVAWEPQNIGLRPKIPMWNACDTAIFTEVSKMVAGESDPEETMASAT
                     ERFNDIVERGWVA"
     gene            1216..2136
                     /locus_tag="VHK28_02455"
     CDS             1216..2136
                     /locus_tag="VHK28_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2141125.1"
                     /translation="MTTAVQSRAVAEETASGPPPGRRRLTFTGRMLTPGVLVLAAISI
                     VPFIALVVMSFSRVRLLGGVRLEWVGLDNWSRALADPDMWSSWAKTAVYLFLTLGLEM
                     VLGFGLALLLYRVTRARGLLLSLVLLPMFVAPVIVGLLGRYLTDSTFGLYAWVLNQLG
                     WDGDILGGTTSAFVAVVLMDVWEWTPLIALIALAGLSSVNPSTLEAASLDGAGYWRSL
                     RHIVLPSISGILLVALLIRAMDAVRYYDIIYITTNGGPADATKTIPIRLFENAFRFFD
                     LGYAATIGLMMLAITILIARGFVSMLQKED"
     gene            2191..3003
                     /locus_tag="VHK28_02460"
     CDS             2191..3003
                     /locus_tag="VHK28_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2141126.1"
                     /translation="MNVLTTFLLLLALAWTLVPLLWMLMGSFKSGADVTSGTPKVLFD
                     PTLENYRNLFAGPNDLTPYLWHSVVAAGVSALLSLVLGTLAGYGLANSRMRGKPHLSF
                     WIISTRMAPIAAVILPLFLMFRLAGLIDTLPGLVLAYMTFNLPFAIWLMSAFFANVPP
                     SIEESALVAGCTRWQAFWHVVLPLSKAGLVTTGVLCLVFAWNDYAFATVFSGPRSQTL
                     PIAASQLVTQTGIDWGQLSAIGTIVVVPMILAGLAVRRWLVTGLTLGAVTGE"
BASE COUNT          455 a         1057 c         1084 g          497 t
ORIGIN      
        1 catcaacgtc aacatcgtca acctggagct ctcggcgctg gtgcagcggg ttgcgctgga
       61 catggcgtcg ggcgcgtcgc agtaccaggt gatctacgcc gacccgtacc aggtgctggc
      121 gccgtaccac aaaggcctgg cagacctgcg cgagttcatc gagggggagg gcttccctcc
      181 gctcgagggc gggatcgacg acttcatccc cacgcagatg gacgccgcgg gccggttcga
      241 gcgggagggc ccgatctacg ccctgcccta cgactgcccc acgatgatct ggcagtaccg
      301 cgcggacctc ttcgacaagt acggccagca gatggccgac gacctcggct tcgaccccac
      361 gcccggcagc gaccggacgt gggaggagta ccggcagatc gcccagtggt tcaacgacaa
      421 cgtcgaggag gttccctacg gcaccggcca ccaggccaag cagcacgact cgctgatgtg
      481 cgacttcagc aacatcctgt gggcctacgg cggcgactac ttccccaacg gccagcaggt
      541 gggcctgatg ggtgtcagcg agccgggccc gtgcctgctc gggtcgcccg agagcatgga
      601 ggcggccgcc ttctacaact ccctgctcga ggtcgcccac cccggctcgg cgacctggga
      661 ctgggacggt gtcggtgcgg cgctgcgcgc cggcgagatc gcgatgtgcc cgaactggca
      721 cgagttcgcc gcgagcaacg aggaggcgct cccgggcaag gtgcactacg ccccgctgcc
      781 gaagggcccc gcccgcgcgg cgcacatgta cggcggcacg ggcatctcgg tggccggcaa
      841 cgccaccggc gccgagcgcg gcgccgcctg gctgttcgtg aactgggcga cggccccgca
      901 gacgcagctc agcaacctgc gcagctcggc cggcggcggc acgccgacac ggacctcggt
      961 gtaccagctg ccggcggtgc gcgccgccga ggagcggccg agcgagatgc ccaacatgct
     1021 gaccgccgac gccgtgagcg tcgcctggga gccgcagaac atcgggctgc gcccgaagat
     1081 cccgatgtgg aacgcgtgcg acaccgccat cttcaccgag gtgtcgaaga tggtcgccgg
     1141 tgagagcgac ccggaggaga ccatggccag cgccaccgag cgcttcaacg acatcgtcga
     1201 gcgggggtgg gtggcatgac gaccgccgtg cagtccaggg cggtcgccga ggagaccgcc
     1261 agcggccctc cgccgggacg acggcggctg acgttcaccg gcaggatgct cacgccgggt
     1321 gtcctcgtcc tggcagcgat ctcgatcgtg ccgttcatcg cgctggtcgt catgagcttc
     1381 agccgcgtcc ggttgctcgg cggcgtccgg ctggagtggg tcgggctgga caactggagc
     1441 cgggcgctcg cagaccccga catgtggtcg agctgggcga agacggccgt ctacctgttc
     1501 ctgaccctcg ggctggagat ggtgctcggg ttcggcctgg cgctgctcct ctaccgggtc
     1561 acacgggcac ggggcctgct gctgtcgctg gtgctgctgc cgatgttcgt cgcgcccgtc
     1621 atcgtgggcc tgctcgggcg ctacctcacc gactccacct tcgggctgta cgcgtgggtg
     1681 ctcaaccagc tgggctggga cggcgacatc ctcggcggca ccacgagcgc gttcgtcgcg
     1741 gtggtgctca tggacgtgtg ggagtggacg ccgctcatcg cgctcatcgc gctcgcgggc
     1801 ctgagctcgg tgaacccgtc gacgctcgag gcggccagcc tggacggcgc gggctactgg
     1861 cgcagcctgc gccacatcgt gctgccgtca atatccggca tcctgctggt cgcgctgctg
     1921 atcagggcga tggacgcggt ccgctactac gacatcatct acatcacgac caacggcggt
     1981 ccggcagacg cgaccaagac catccccatc cggctgttcg agaacgcctt ccggttcttc
     2041 gacctcgggt acgccgccac catcgggctg atgatgctcg ccatcacgat cctcatcgcc
     2101 cgcggcttcg tcagcatgct gcagaaggag gactgagccg tgtcgaccgc cggtgtgagc
     2161 ctgcacagct caccgtcgaa ggaacgcgag atgaacgtcc tgacgacgtt cctcctcctg
     2221 ctggcactgg cgtggacgct ggtcccgctg ctgtggatgc tgatgggctc gttcaagagc
     2281 ggcgcggacg tcacgtccgg aacgccgaag gtgctcttcg accccacgct ggagaactac
     2341 cgcaacctgt tcgccggccc gaacgacctg acgccgtacc tgtggcacag cgtggtcgcc
     2401 gccggcgtct ccgcgctgct gtccctggtg ctgggcacgc tcgcaggcta cgggctggcg
     2461 aactcgcgga tgcgggggaa gccgcacctg tcgttctgga tcatctcgac gcgcatggcg
     2521 ccgatcgccg cggtgatcct tccgctgttc ctgatgttcc ggctggcggg gctgatcgac
     2581 acgttgccgg ggctggtgct cgcgtacatg acgttcaacc tgccgttcgc catctggctg
     2641 atgagcgcgt tcttcgccaa cgtcccgccc tccatcgagg agtcggcgct ggtggccggc
     2701 tgcacccggt ggcaggcgtt ctggcatgtg gtactgccgc tgagcaaggc ggggctggtc
     2761 accaccgggg tgctgtgcct ggtgttcgcc tggaacgact acgcgttcgc cacggtgttc
     2821 agcggcccga ggtcgcagac cctgccgatc gccgccagcc agctggtcac gcagaccggc
     2881 atcgactggg ggcagctgag cgccatcggc acgatcgtgg tggtgccgat gatcctggcc
     2941 gggctggcgg tgcgccggtg gctggtcacc gggctgacgc tgggggctgt gacaggtgag
     3001 tgacggcgac gttccgcgga ccatgcgcgc cagcgtgctg cacgcacccg gcaagctcgc
     3061 ggtggaggag cgcccggtgc cggcaccggg tac
//
LOCUS       DATACA010000118         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2747509, whole genome shotgun sequence.
ACCESSION   DATACA010000118 DATACA010000000
VERSION     DATACA010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747509"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VHK28_02465"
     CDS             <1..752
                     /locus_tag="VHK28_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141127.1"
                     /translation="GQPEVARRAAHRGVALNPGDGPGWTNLALLCLGAGERDCAATAA
                     DGAIARSTTRQELVNAALVHEALGNHQAADRAYRLSALTNFWTTITVPWPRPVSFVAA
                     DVAGLEELGDTVQFNEVIALGVLRLPIEPDAYTNVHARILGHAFVGDYDQVALELDAA
                     SSAARAAPLTWELAALLARPLGRDPEEALRIAQVARGIPPRNEPPSPAGLAFDIATFR
                     AYPGDELVAAAERLIPEVPWPWVLEPYLPAP"
     gene            complement(762..1451)
                     /locus_tag="VHK28_02470"
     CDS             complement(762..1451)
                     /locus_tag="VHK28_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141128.1"
                     /translation="MPAEQLPLPVPPDDHSRQRLPSRVAPMQPTAVDEPFDDPAYLFE
                     PWWPGVRAFAFAERGILRLQVDGLADALAAFPELAALGAQLLEDGVLLDGTLLVLDEA
                     GRPDPALLRERLDGRARSGSPAYVASDLLWAGGQPAVRRSFSVRRRWLEAILPASDRI
                     TVGRGYVGDGTLVAEALAELGIDGLSARRLASRYRAGPAGDAWLRAPIQPAEPRQRPT
                     LALILRLPLGD"
     gene            complement(1462..2454)
                     /locus_tag="VHK28_02475"
     CDS             complement(1462..2454)
                     /locus_tag="VHK28_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2141129.1"
                     /translation="MARSMWRGAIQFGLVTIPVRLYLATETGGIGFNMLHESCLNRIQ
                     MKVYCPYHDEIIPRSETVRGYEWSKGKYVVVTEEDLESVPLKTVRSIEIEAFVGEEEA
                     EETGSRFVKQAYYLEPDEIGRKAFYLLKSVLAETGKTAICKIVLKDREQLAALNPFSN
                     TMLLTTLHWPDEVRSLEELNLPEDQVDIKPAERKMAEQLIASMADEFDPAAYRDEYRE
                     ALMGVIEAKVAGEKPEPARKAEPTKITDLMAALEASVNAARDARRTGVEEDGKETKGA
                     RATKGEKQSKAEKPSKAPRKAAAASKDGRKASGRGRKADRDAEEVPAARRRKTA"
     gene            2576..3013
                     /locus_tag="VHK28_02480"
     CDS             2576..3013
                     /locus_tag="VHK28_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141130.1"
                     /translation="MSEQVARSVPSTATALSADGEEQRAYVLEDDADIAALEADILRD
                     LGYSVVVCTRIVELGDLMALALPHLMVVDVMLPDGDGGDITELLQGAWPGIPVVFVSA
                     ASRERLVALEPFGPVVAKPFDIEAFTSAVQSAVQTPRALRSAS"
BASE COUNT          416 a         1133 c         1117 g          480 t
ORIGIN      
        1 gcggccagcc ggaggtggcc cgccgtgcgg cgcatcgggg agtggcgctc aacccgggtg
       61 acggaccggg gtggaccaac ctggcgctgc tctgcctggg cgctggggag cgcgactgcg
      121 ccgctaccgc ggctgacggc gcgatcgccc gctccacgac acgccaggag ctggtgaacg
      181 ctgccctggt gcatgaggcg ctcggcaacc accaggccgc cgatcgcgcc tatcggctgt
      241 cggcactcac caacttctgg accacgatca ccgtcccgtg gccgcggccg gtgtccttcg
      301 tcgcggccga cgtcgcgggc ctggaggagc tcggtgacac ggtgcagttc aacgaggtga
      361 tcgcgttggg cgtcctgcgc cttcccatcg agccggatgc ctacaccaac gtccatgccc
      421 ggatcctggg gcatgccttc gtgggcgact acgatcaggt cgcgctcgag ctggacgctg
      481 cgagctcggc cgcccgcgcg gcgccgctga cctgggagct ggccgcgctg ctggcgcggc
      541 cgctgggtcg cgacccggag gaggcgctgc gcatcgccca ggtggcccgg ggaataccgc
      601 cgcgaaacga gccgcccagc cccgcgggcc ttgccttcga catcgcgacg ttccgggcct
      661 atccggggga cgagctggtg gccgccgccg aacgactcat tcccgaggtg ccctggccgt
      721 gggtgctgga gccgtacctg cccgctccgt agcggccggc ctcagtcgcc gagcggaagt
      781 cgcaggatga gcgccagggt cgggcgctgc cgcggctcgg cgggctggat gggcgcccgc
      841 agccaggcgt cgccggccgg gccggcccgg tagcgcgagg ccaggcggcg cgccgagagg
      901 ccgtcgatgc ccagctcggc gagcgcctcg gccaccagcg tcccgtcgcc cacgtagccc
      961 cggccgacgg tgatgcggtc ggaggccggg aggatggcct caagccatcg gcgacggacg
     1021 ctgaaggagc ggcgcacggc cggctgaccc ccggcccaca gcaggtcgga ggcgacgtac
     1081 gccggtgagc cgctcctggc gcggccatcc aggcgctccc gcagcagggc ggggtccggg
     1141 cggccggcct cgtcgagcac cagcagcgtg ccgtccagca gcaccccgtc ctcgagaagc
     1201 tgggcgccca gggctgccag ctcggggaac gcggccagcg cgtccgccag gccgtccacc
     1261 tgaaggcgga ggatgccgcg ctcggcgaac gcgaaggcac ggacgcctgg ccaccacggc
     1321 tcgaagaggt aggcggggtc gtcgaacggc tcatcgaccg cggtcggctg catgggcgcc
     1381 acccgcgacg ggagccgctg ccgcgaatga tcgtcgggcg ggacggggag cggaagctgc
     1441 tcggccggca ccctcgctgc gtcaggcggt cttgcgacgg cgggccgccg ggacctcctc
     1501 ggcgtcgcga tcggccttgc ggccccgtcc ggaggccttg cgaccgtcct tcgatgcggc
     1561 cgcggccttg cgtggtgcct tggacggctt ctcggccttg gactgcttct cacccttggt
     1621 cgccctggcg cccttcgtct ccttgccgtc ctcctcgacg ccggttcgcc gcgcgtcgcg
     1681 cgccgcattc acgctggcct cgagcgcggc catcaggtcg gtgatcttgg tcggctcagc
     1741 cttgcgcgcc ggctcgggct tctcgccggc caccttggcc tcgatgacgc ccatcagcgc
     1801 ctcgcggtac tcgtcgcggt aggcggccgg atcgaactca tcggccatgc tggcgatcag
     1861 ctgctcggcc atcttgcgct ccgccggctt gatgtcgacc tggtcctccg ggaggttgag
     1921 ctcctcgagc gaacggacct catcgggcca atgcagggtg gtcagcagca tggtgttgct
     1981 gaacgggttg agcgcggcca gctgctcgcg gtccttgagc acgatcttgc agatggccgt
     2041 cttgccggtt tcggccagga cggacttgag gaggtagaag gctttgcggc cgatctcatc
     2101 gggctcaagg tagtacgcct gcttgacgaa gcgcgacccg gtctcctccg cctcctcctc
     2161 gcccacgaag gcctcgatct cgatggagcg caccgtcttg agcggcaccg actcgaggtc
     2221 ctcctcggtg accaccacgt acttgccctt gctccactcg tagccgcgca cggtctccga
     2281 gcgcgggatg atctcgtcgt ggtacgggca gtagaccttc atctggatcc ggttcaggca
     2341 gctctcgtgg agcatgttga agccgatgcc gccggtctcg gtggccaggt agagccgcac
     2401 cgggatggtg accagcccga actgaatcgc cccccgccac atggatcgcg ccatcagacc
     2461 ctacctgtcc tcacctgtct tccggggcgc catcctacac cgggcgagca agcacacccg
     2521 attaggacca ttccgtcatt gccgctcgcc gttggcgaac ggcagactgc gacgcatgag
     2581 cgaacaggtc gcaaggagcg tgccgtccac cgcaaccgcc ctcagcgcgg acggcgagga
     2641 gcagcgggcc tacgtcctgg aggacgacgc ggatatcgcc gccctggagg cggacatcct
     2701 gcgcgacctg ggctactcgg tggtggtctg cacccggatc gtggagctcg gcgacctgat
     2761 ggccctggcc ctcccgcacc tgatggtggt ggacgtcatg ctgcccgacg gggacggcgg
     2821 cgacatcacc gagctcctgc agggcgcctg gccgggcatc ccggtcgtct tcgtctcggc
     2881 agccagccgc gagcgcctgg tcgcgctcga gccgttcggc ccggtcgtgg ccaagccgtt
     2941 cgacatcgag gcgttcacca gcgccgtgca gagtgcggtt cagacgccgc gggcgcttcg
     3001 cagcgcctcg tgatcggggt ccacagccgg tcagccgggc ggcgggccgg tgaccaccac
     3061 cagcgccgcc tgtccctcgc catcggtgtc ggtccgcgtg ccatccgcgc catcggccca
     3121 cagccggtag cgatgctcga tgcgca
//
LOCUS       DATACA010000119         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2266336, whole genome shotgun sequence.
ACCESSION   DATACA010000119 DATACA010000000
VERSION     DATACA010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266336"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VHK28_02485"
     CDS             complement(<1..514)
                     /locus_tag="VHK28_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2141131.1"
                     /translation="MEILVLGGGGREHALAWRLARDEAVRRVRIAPGNGGTPATAETV
                     PGLDPLDPVAVARHAGRERYDLVVIGPEGPLASGVADALAQAGIPAFGPSRDAARLES
                     SKAFSKEQMRRAGVPTAESADFVTVDDALGHARRMDPPPVVKADWLAAGKGVVVPDSA
                     EEAEIAIRTLF"
     gene            complement(514..>2541)
                     /locus_tag="VHK28_02490"
     CDS             complement(514..>2541)
                     /locus_tag="VHK28_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2141132.1"
                     /translation="LTVVAVGAAALLLLNIWFRTLWALALAGGAWFVLSVLVGSVYPT
                     FVQNFNVNPNEQNLERPYIADHIASTRAAFELDEIEVRDFSGEGRLSEGVFEGDAPTV
                     DNLRLCDYRPLLTTFGQQQIIYPYYDFLDVDIDRYQIDDEQRQIMLSGRELNIDKLAA
                     DARTWTNERLVYTHGFGITAVPVDAVTRQGQPDYLVSGINREPSLPVGQPRIYFGEAT
                     TTWVVTGTGTEEFDYPLDEAADVATTTWQGTTGVDIDNPIARFLFAARFGDLNLIISN
                     QLTDDSQILFRRSIYERVAELAPFLAYDGDPYLVSADDRLMWVWDAYTATGRYPNAQP
                     LGAGSPFAGANYVRNSVKVVIDAYDGTVRFFVADPDEPIVRAYANIFPSLFEPMDGLP
                     SGLEPHLRYPEDLFIAQNEAYQLYHLPPTDGGANTYYSQNDRWAIPPDPTQGSGTPME
                     PYYVIMRIPGEDEAEFVLIQPLVLERRPNMIAWVAARMDPGVYGEKIAFHFPNDTSTD
                     GPVQVQARIDQDPTISAQFSLWDRTGSSVIKGNLLVLPIGEDSLVYVQPIFLQAEGAP
                     FPEFVRVIMVSEEQVAFAEDIPGALEQLLGEAEPPPPDGGPGGPPADLAGLVAEAQRL
                     YEEAQAALAAGDLGTYQARLNELEPILEALAELTGAEPDPESPEPEASPSP"
BASE COUNT          371 a          864 c          915 g          391 t
ORIGIN      
        1 cgaagagggt ccggatggcg atctccgcct cctccgcgct atcgggcacc accacgccct
       61 tgccggccgc cagccagtcc gccttgacca ccggcggcgg atccatgcgc cgcgcgtggc
      121 ccagcgcgtc gtcgaccgtg acgaagtcgg cgctctccgc ggtcggcacg ccggcgcggc
      181 gcatctgctc cttggagaac gccttcgagg actccagccg cgccgcatcc cgcgagggac
      241 cgaaggccgg gatgccggcc tgcgccagcg catcggccac cccggacgcc agcggcccct
      301 ccggcccaat gaccaccagg tcgtaccgct cccgcccggc gtgccgcgcc acggccaccg
      361 gatcgagcgg atccaggccc gggaccgtct cggccgtggc cggcgttcca ccgttgccag
      421 gggcgatccg cacccgccgc accgcctcgt cccgggccag gcgccaggcc agggcgtgct
      481 cccgcccacc gccgcccagg accaggatct ccatcagggc gaggggctcg cctcgggctc
      541 cggcgactca gggtccggtt ccgcgccggt cagctcggca agggcctcca ggatcggctc
      601 gagctcgttg aggcgagcct ggtaggtccc caggtcgccg gcggccagtg ccgcctgcgc
      661 ctcctcgtac aggcgctgcg cctcggccac caggccggcc aggtcggcgg gtggtccgcc
      721 gggaccgcca tccggcggcg gaggctcggc ctcgccgagc agctgctcca gcgccccggg
      781 gatgtcctcg gcgaaggcga cctgctcctc gctgaccatg atgacccgca cgaactcggg
      841 gaagggggcg ccctccgcct gcaggaagat gggctggacg tatacaaggc tgtcctcgcc
      901 gatgggcagc accagcaggt tgcccttgat gaccgatgag ccggtccgat cccacagcga
      961 gaactgggcg ctgatggtcg gatcctggtc gatgcgtgcc tgcacctgga ccggcccgtc
     1021 ggtggaggtg tcgtttggga agtggaaggc gatcttctcg ccgtagacgc cggggtccat
     1081 gcgggccgcg acccaggcga tcatgttcgg ccggcgctcg agcaccagcg gctggatcag
     1141 gacgaactcg gcctcatcct cgccggggat gcgcatgatc acgtagtagg gctccatggg
     1201 cgtgccgctg ccctgggtcg gatcgggcgg gatggcccag cggtcgttct ggctgtagta
     1261 ggtgttcgcc ccgccatcgg tcggcggcag gtggtagagc tggtaggcct cgttctgggc
     1321 gatgaacagg tcctcggggt agcgcaggtg cggttccagc ccgctcggca ggccgtccat
     1381 gggctcgaac agggacggga agatgttggc gtaggcgcga acgatgggct catcggggtc
     1441 ggccacgaag aagcgcacgg tgccgtcgta ggcgtcgatc accaccttca cgctgttgcg
     1501 cacgtagttg gccccggcga aggggctgcc agcgcccaac ggctgcgcgt tggggtagcg
     1561 gccggtggcg gtgtacgcat cccagaccca catcaggcgg tcatcggcac tgaccaggta
     1621 cggatcgccg tcgtaggcca ggaagggcgc cagctcggcg acgcgctcat agatcgagcg
     1681 gcggaagagg atctgggagt catcggtcag ctggttgctg atgatcaggt tgaggtcgcc
     1741 gaagcgcgcc gcgaacagga agcgggcgat cgggttgtcg atgtcgaccc cggtggtccc
     1801 ctgccaggtg gtggtcgcca cgtccgccgc ctcgtccagc ggatagtcga actcctcggt
     1861 gccggtgccg gtcaccaccc aggtggtcgt cgcctcaccg aaatagatgc gcggctgccc
     1921 gaccggcagc gacggctcac ggttgatgcc gctgacgagg tagtcgggct ggccctggcg
     1981 ggtgaccgcg tccaccggca cggcggtgat cccgaatccg tgggtgtaca ccagccgctc
     2041 gttggtccag gtgcgcgcgt cggccgccag cttgtcgatg ttcagctcgc ggccgctgag
     2101 catgatctgg cgctgctcgt cgtcgatctg gtagcggtcg atgtccacgt cgaggaagtc
     2161 gtagtagggg tagatgatct gctgctggcc gaaggtggtg agcagcgggc ggtagtcaca
     2221 cagccgcagg ttgtcgacgg tcggcgcgtc accctcgaag accccctcac tgagccggcc
     2281 ctcgccgctg aagtcgcgga cctcgatctc gtcgagctcg aatgcggcgc gtgtggaggc
     2341 gatgtggtcc gcgatgtagg gtcgctccag gttctgctcg ttggggttga cgttgaagtt
     2401 ctgcacgaag gtcgggtaca cgctgccgac cagcaccgac agcacgaacc aggcaccgcc
     2461 ggccagcgcc agcgcccaca gggtccggaa ccagatgttg agcaggagca gggcggccgc
     2521 gccgaccgcc accaccgtca g
//
LOCUS       DATACA010000120         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2025617, whole genome shotgun sequence.
ACCESSION   DATACA010000120 DATACA010000000
VERSION     DATACA010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025617"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VHK28_02495"
     CDS             <1..899
                     /locus_tag="VHK28_02495"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012576158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX2141133.1"
                     /translation="ARPQVTVLGSINVDLVVSVPRLPRPGETVLGGTFAQHMGGKGAN
                     QAVAAARAGARVVMVGAVGADANGDASLAALAHEDVDVSHVRRVQDPTGVALIAVAPD
                     GENQITVAPGANRALQADDVPDEVLASSQVLLASLEIPLPMVTAAMRAAARLGLRRVL
                     NPAPAQPLPDDLLTLRPVLTPNREEALVLAAVDDLDTAVERLAAHGPLVVTLGSEGAL
                     VVENGVRTPIPAHRVERVADATGAGDTFAGVLATWLAGGSGLLEAATAANAAAALSVQ
                     QAGARGGMPRAEQIRAALAAGA"
     gene            complement(905..1081)
                     /locus_tag="VHK28_02500"
     CDS             complement(905..1081)
                     /locus_tag="VHK28_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141134.1"
                     /translation="MTVGSTQPDGSSDPSPDDAREWAIPEDLWPDGFDDPWAASGDEP
                     WDPEHPFQYPVGLP"
     gene            complement(1110..1778)
                     /locus_tag="VHK28_02505"
     CDS             complement(1110..1778)
                     /locus_tag="VHK28_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311993.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX2141135.1"
                     /translation="MRSRQPEPADGLSGEEREEAIQRARSLDEVRRAAERCTACDLYR
                     NATQTVFGAGGERARLLFLGEQPGDMEDRAGEPFVGPAGRLLNEALDAAGIARPDAYV
                     TNVVKHFKWRRAPSGKRRIHQKPDRAEIGACRPWLEAEVARVQPDLIVCLGATAAQAL
                     LGAAFRVTRQRGSVHPTDLGPPALATVHPSSVLRSRSSDERQAAFDGLVADLAVARGV
                     LEGR"
     gene            complement(1750..1953)
                     /locus_tag="VHK28_02510"
     CDS             complement(1750..1953)
                     /locus_tag="VHK28_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141136.1"
                     /translation="MDATERLRRESPGRYRTDDGRFRVESSDGAWYVTDQETRDELGQ
                     AKLSGPFATLGEARHAVAAARAR"
     gene            2055..>3000
                     /locus_tag="VHK28_02515"
     CDS             2055..>3000
                     /locus_tag="VHK28_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2141137.1"
                     /translation="MRIALVSGPMVPIPPPRYAGTERIVAVLADELSRRGHEVTLIGP
                     GDSKVECELIPSIDQAMWTSGVRGNPTPYVQVTIERAWSVCERFDVIHSHLDTLSFSF
                     ARHCPTPVVHTLHGRLDMPGVPELLDEFRDIPLVAISDSQGRWWPKNNWQATVHHGLP
                     LENAPFSAEPGGYLAFVGRVAPEKGIEAVVTLARETAIPLRMAAKVFEIEEQELFDRV
                     VAPAVEEGVVSFEGELPTDERDDLMRGALAVVMLGAWPEPFGLVAIESMATGTPVIAR
                     RAGALPEIVEHGRTGFLVDDLTEACLAVRSVAELDRTAI"
BASE COUNT          418 a         1077 c         1071 g          434 t
ORIGIN      
        1 aggcgcgccc gcaggtgacc gtcctcggct cgatcaacgt ggacctggtg gtcagcgtgc
       61 cgcgcctccc acgccccggc gagacggtgc tgggcggcac gttcgcgcag cacatgggcg
      121 gcaagggcgc caaccaggca gtcgccgcag cccgcgccgg cgcgcgggtc gtcatggtcg
      181 gcgcggtggg cgccgatgcc aacggtgatg cctctctggc cgcgctggcg cacgaggacg
      241 ttgatgtgag ccacgtgcgg cgcgtccagg acccgaccgg ggtggccctc attgccgtgg
      301 cgccggacgg cgagaaccag atcacggtgg cgccgggcgc caatcgcgca ctgcaggcgg
      361 acgatgtccc ggatgaagtg ctggcctcgt cgcaggtgct tttggcctcg ctcgagattc
      421 cactgccaat ggtgaccgcg gcgatgcgcg ccgccgcacg cctgggactg cgccgcgtgc
      481 tcaatcccgc gccggcgcag ccgctccccg acgacctgct aacgctgcgc ccggtattga
      541 cgcccaaccg cgaggaggcc ctcgtgctcg ccgccgtcga cgacctcgac actgcggtgg
      601 aacggctcgc agcccacggg ccgctggtcg tcaccctcgg tagcgaaggc gcgctggtcg
      661 tggagaacgg ggtgcgcact cccattcccg cccaccgggt agagcgggtc gcggacgcca
      721 ctggggccgg tgacaccttc gccggcgtgc tggcgacctg gctggctggc ggaagcgggt
      781 tgctggaggc cgcgacggcg gccaacgcgg cggcggcgct atcggtccag caggctggag
      841 cgcgtggcgg catgccccgc gccgagcaga tcagggctgc attggcagcc ggagcctgac
      901 gcccctacgg caacccaacc gggtactgga aggggtgctc gggatcccag ggctcgtcgc
      961 cgctggctgc ccatggatcg tcgaagccat cgggccagag atcctccgga atcgcccact
     1021 cacgggcgtc gtccggtgac gggtctgatg acccgtccgg ctgcgtgcta ccgaccgtca
     1081 tctcgaatcc cccaattgtt gacgtgccgc taccggccct ccagcacgcc acgcgccacc
     1141 gcgaggtcgg cgaccaggcc atcgaatgcc gcctgccgct cgtcggagga gcgcgaacgc
     1201 aggaccgatg acggatgcac ggtggccagt gccggcgggc cgaggtcggt cggatggacg
     1261 ctcccccgct gccgcgtgac gcggaacgcc gccccgagca gcgcctgtgc ggcggtggcg
     1321 cccaggcaga cgatgaggtc tggctgcaca cgagccacct ccgcctccag ccaggggcgg
     1381 caggcgccga tctccgcccg atccggcttc tggtgaatgc gtcgcttccc ggagggagca
     1441 cgacgccact tgaagtgctt gaccacgttc gtcacgtagg catccggtcg ggcgatgccg
     1501 gccgcgtcca gcgcctcgtt cagcaggcgc ccggccggtc ccacgaacgg ctccccggct
     1561 ctgtcctcca tgtcacccgg ctgctcgccc aggaacagga ggcgcgcacg ctcgccaccg
     1621 gcgccgaaca ccgtctgggt ggcgttgcgg tacagatcac aggcggtgca tcgctccgcg
     1681 gcacgccgca cctcgtccag cgatcgggcg cgctggatgg cctcctcgcg ctcctcgccg
     1741 gacagcccgt cagcgggctc gggctgccgc gaccgcatgt cgtgcctccc cgagggtggc
     1801 gaacggcccg ctcagcttgg cctgccccag ctcgtcgcgt gtctcctggt cggtcacgta
     1861 ccaggcgccg tcgctcgact cgacgcggaa gcggccatca tcggtccggt agcggccagg
     1921 agactcccgg cgcaggcgtt cggtggcgtc catcggtccc tgcgcaggca agcacaccgc
     1981 gtgccggggg gtggcccgga ttgtgcttgc caagggacaa gcccgcccac cggcctcgtt
     2041 acagggggat tgcgatgcgc atcgcactgg tatccggacc gatggtgccc atcccgccgc
     2101 cccgctatgc cggcacggaa cggatcgtgg cggtcctggc ggacgagctg tcgcggcgcg
     2161 gccacgaagt caccctcatc ggtcccggcg actcgaaggt ggagtgcgag ctcatcccgt
     2221 ccatcgacca ggcgatgtgg acctccggcg tgcgcggcaa cccgacgccc tacgtccagg
     2281 tcaccatcga gcgggcctgg agcgtgtgcg agcggttcga cgtcatccac tctcacctcg
     2341 acacgctcag cttcagcttc gcgcgccact gcccgacgcc ggtggttcac accctgcacg
     2401 gccggctgga catgcccggc gtgcctgagc tgctcgacga gttccgcgac atcccgttgg
     2461 tggccatcag cgacagccag ggccgatggt ggccgaagaa caactggcag gcaaccgtgc
     2521 accacggcct gccgctggag aacgccccat tctccgcgga gcctggcggc tacctggcct
     2581 tcgtcggtcg ggtggcgccg gagaagggca tcgaggcagt ggtcacgctg gcgcgggaga
     2641 ccgccatccc gctgcgcatg gcggccaagg tcttcgagat cgaggagcag gagctcttcg
     2701 accgggtggt ggcgccggcc gtggaggagg gcgtggtcag cttcgagggc gagctgccga
     2761 ccgacgagcg cgacgacctc atgcgcggcg ccctggctgt cgtgatgctg ggtgcctggc
     2821 cggagccgtt cggcctggtg gcgatcgagt cgatggccac cggcacgccc gtcatcgccc
     2881 gccgcgccgg ggcgctgccc gagatcgtgg agcatggccg gacaggcttc ctggtggacg
     2941 atctgaccga ggcctgcctg gccgtccgat cggtggctga gctggaccgc acggcgatcc
//
LOCUS       DATACA010000121         7445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2427004, whole genome shotgun sequence.
ACCESSION   DATACA010000121 DATACA010000000
VERSION     DATACA010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427004"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VHK28_02520"
     CDS             complement(<1..823)
                     /locus_tag="VHK28_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2141138.1"
                     /translation="MPDFKVNAPFQPTGDQPQAISALAGGVRSGLKHQVLLGATGTGK
                     TATAAWLIQEVQKPTLVMAHNKTLAAQLYQEFKEFFPENAVEYFVSYYDYFQPEAYLP
                     RSDTYIEKDSSRNDEIDRLRHNATRALFERRDVIVVASVSCIYGIGAPIDYGSTVLNL
                     KVGGRYRRDGVLRHLVDLQYQRNDAALARSRFRVRGDVLEFAPVSGDYIVRVDFFGDE
                     VERIVEVDELTGEVLAERREVNIYPATHYVTPRDKLLLAIDDIEAEMEERVGQLAA"
     gene            1058..1591
                     /locus_tag="VHK28_02525"
     CDS             1058..1591
                     /locus_tag="VHK28_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX2141139.1"
                     /translation="MVEFAFVLLPVLLLIVGIVQFGLLFGANVTLTNAAREGARAGTI
                     YVYDHSHTKAWNDAQRCGDVAQAAMQSFGILSTSGPHFSVTLTGGACPTPAGDGQTNG
                     DLVISYCEYVDTPDGPCPDTSDSDTNCTPDTRQSCLLRVTLTYRSDIVVPLLGSVLST
                     DASGRFVQSATATMVVN"
     gene            1591..3513
                     /locus_tag="VHK28_02530"
     CDS             1591..3513
                     /locus_tag="VHK28_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX2141140.1"
                     /translation="MAPRPEPRGQVLVLFAILLVVLLAFTGLAIDVGRQNAEQRYIQT
                     AADAAALAACQALIDGDTDVVAATEARTVAEINLQNSPAGASAVIAPDAARIYADGHA
                     GDPAYLVSGILISGTTVRVAISSTLGTTLARVVGVTTMEAGARSRCQLQGGPAIPIVA
                     RRYENAPGPSNGFRDFLATEATSTIGQVDAWNVLGYDGRTPASEVERGPEFELYGPNA
                     KASNDSSFRGFVALDVRNFQDLTSRVYYNGVVPGVSENTLKSKEGAYLLSGYPGPGFP
                     PISQPADPNDQVATLSGNDTAMVVGNFRDVYEVGDRILLAVYNGTVMQIPDFAISPPS
                     VIIVPSTTTTPVAGPNFSVTRNDAFNSTVTLHLHGDAEATAQGHPEWDLVPTPAITPP
                     AAGDMNEPTWSTNVFIPNKNGTTVSMSGIQTNNVPAGIYTVWLEGHSGDPYFQTRRAP
                     VPVRVGGATRDFSLVNSTTSASIASVGGSAVLPIFVSTTNASSTKWNTTNPVTLSVDQ
                     SSFTDCSLVPKTIGAGQLTLSQSSLVPTSSGDGAQSNLTISSAGLAPGCYRFNLRAYG
                     TNGDGQPVVHLHPVTFTVATTSSDGTYVDIIGFAVFEITTLDANSIAGRAVSGVYADP
                     NDVALRRAQQARLMPW"
     gene            3533..4354
                     /locus_tag="VHK28_02535"
     CDS             3533..4354
                     /locus_tag="VHK28_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEX2141141.1"
                     /translation="MELEYTDKNSRRSKLYIAVGLVMALAVAGMVYAALQFSGITKDN
                     DVVMRQVVVAARDIASRKPIEEGDVTMTTVVANETNASAFTRIDEVLGRISGVPILTN
                     QMLTQNMLASTTEGQSYTILELGEEFDPNGPDLRAVSISVADDRAVAGTLVPGQVVDV
                     IVTMAINPEVGQTPEEAEVTTAQFIPGPSTKVTFQALTILARSGGLYIMRTDLATAEQ
                     IAEVTAAGGQFTLALRPDVDDRTADTEGSTIDRLIEEFEFPVPLPPDFEAEAAGR"
     gene            complement(4361..4942)
                     /gene="coaE"
                     /locus_tag="VHK28_02540"
     CDS             complement(4361..4942)
                     /gene="coaE"
                     /locus_tag="VHK28_02540"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX2141142.1"
                     /translation="MKVIGLTGNIGCGKSTVAAMLSEIGVAVIDLDAVARQIRNNDAE
                     ARRRIEARFGTIDAGQLASVVFSDPEALHDLELILHPLVRNETRARLAELEAAGVEAA
                     CIEAIKLLESPLSDRCDQTWVVRCDEADAVQRVMATRGMTDEQARARLASQSSQEEKV
                     RAADVIIDGSAALEETRRQVETAYRALVGADAP"
     gene            complement(4939..5841)
                     /gene="mutM"
                     /locus_tag="VHK28_02545"
     CDS             complement(4939..5841)
                     /gene="mutM"
                     /locus_tag="VHK28_02545"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX2141143.1"
                     /translation="MPELPEVETVARDLQRRVAGARITDAEVHWGRTITNPEPDEFVA
                     MVRGAEISRVGRRAKSVLIHLADGRVMTVALRMTGALILAQAGADADPYARVVFRLAD
                     GRELRYRDVRKFGRIGLWEGGGLRARTKRPRKTRQGRTRLPFRVGDVFGRHGPEPLAS
                     GFSAARFAERLRGRSARLKSLLLDQSFVAGVGNIYADEALWRARLHPLRRADTLTPAE
                     VRRLHRAVRAVLRQGIENRGASFSDYVDTDAEPGDNAERLYVYRRTGQPCLRCGTPIQ
                     RIVVGQRSTHFCPRCQPEPAGEDG"
     gene            complement(5869..>7445)
                     /gene="polA"
                     /locus_tag="VHK28_02550"
     CDS             complement(5869..>7445)
                     /gene="polA"
                     /locus_tag="VHK28_02550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX2141144.1"
                     /translation="VRGAEVAGPADDTLVASYMVNPSLRAQTLEDLAANRFGATLPER
                     PVIAETGAEEQVAARRAAAEALTALLVRPQLEAELRDGGLLDLYREVEMPLLPVLARV
                     ELAGVVIDREALAGMSAEFGAQLAGLEARIYELVGRPFNIGSPKQLEQVLFHELGLPA
                     TKRTRTGFSTDASVLEELREQHEVVGLILEHRQISKLKGTYVDALPTLVGPDGRLHTT
                     YQQAVAATGRLSSVDPNLQNIPIRTALGRRIRSAFVAGPGKLLLGADYSQQELRILAH
                     VSGDEGLKTDFAAHEDIHLAAAARVLGLPADKVGPRERSIAKMINYGIAYGLSDFGLA
                     DRLKIPREDAQRFIADYFAAYPGIRRYTVEIRMLARDQGFVTTLLGRRRYLPELSARN
                     SALRSAGERMAINMPIQGTAADGMKIAMVRLDAAMRERGLRSRMLLQVHDELVFETDE
                     DELPALAELATQVMEAALPLDPPLEVDLKVGTDWENMDRYVRGDAGGWRRVPKSAAQV
                     AREEAEEEVAAGALVP"
BASE COUNT         1136 a         2715 c         2472 g         1122 t
ORIGIN      
        1 cggccgccag ctgcccgacg cgctcctcca tctcggcctc gatgtcgtcg atggccagca
       61 gcagcttgtc ccgcggcgtg acgtagtggg tggccggata aatgttcacc tcgcgtcgct
      121 cggcgagcac ctcgccggtc agctcgtcca cctcgacgat gcgctccacc tcgtcgccga
      181 agaaatcgac ccgcacgatg tagtcgccgg agaccggtgc gaactccagg acgtcaccgc
      241 gcacgcggaa ccgcgagcgc gccagggccg catcgttgcg ctggtactgc aggtcgacca
      301 ggtggcgcag cacaccgtcg cgacggtatc ggcccccgac cttgagattg aggacggtcg
      361 agccgtagtc gatgggtgca ccgatgccgt agatgcacga cacggacgcc acgacgatca
      421 cgtcccggcg ctcgaagagc gcccgcgtgg cgttgtgccg cagccggtcg atctcgtcgt
      481 tccgggacga gtccttctcg atgtacgtat cggaccgggg caggtaggcc tccggctgaa
      541 agtagtcgta gtagctgacg aagtactcga cagcgttctc cgggaagaac tccttgaact
      601 cctggtagag ctgggccgcc agtgtcttgt tgtgcgccat caccagggtc ggcttctgga
      661 cctcctggat gagccaggca gcggtcgccg tcttgcccgt cccggtggcg cccagcagca
      721 cctggtgctt gaggcccgag cgcacgccac cggccagcgc ggagatcgcc tgcggctgat
      781 ccccggtcgg ctggaacggc gcgttgacct tgaagtcggg catcgcgagc ggcggacctc
      841 tcgttcaggt gggggtaccg cgattctacc gaaacctcca tcggtaccga tggcggccta
      901 cccgagcctc ctataggact ccggggtccc ccttcctacg cttgccagac cgatgcacac
      961 gccagccagg cccttcgtcc ggcgagcgcg cccggccatc atggggtccg ggcgtcggcg
     1021 cctgcccgtc tccacgcggt cgacgcgagg ccaggccatg gtcgagttcg ccttcgtcct
     1081 cctgccggtc ctgctgctga tcgtgggcat cgtgcagttc ggcctcctgt tcggcgcgaa
     1141 cgtcacgctc accaatgctg cgcgtgaggg tgcgcgcgcg ggcaccatct acgtctacga
     1201 ccactcgcac accaaggcct ggaacgacgc tcagcgctgt ggcgacgtgg cgcaggccgc
     1261 gatgcagtcg ttcgggatcc tctcgacgtc cgggccgcac ttcagcgtga cgctgaccgg
     1321 cggcgcctgc cccacacctg ccggcgacgg gcagacgaac ggggacctcg tcatcagcta
     1381 ttgcgagtat gtcgatacgc cggacggccc ctgcccggac acctccgact cggacaccaa
     1441 ctgcacgccg gacacgcgac agagctgcct gctgcgggtg accctgacgt atcggtcgga
     1501 catcgtggtg ccgctcctcg ggagcgtgct gtcgaccgat gccagcggcc ggttcgtgca
     1561 gagcgcaacc gcgaccatgg tggtcaactg atggcccctc gccccgagcc tcgcgggcag
     1621 gtgctggtcc tgttcgccat cctgcttgtc gtgctgctgg cgttcaccgg cctggccatc
     1681 gacgtcggac gccagaacgc ggagcagcgc tatatccaga ccgccgccga tgcggcggca
     1741 ctcgccgcct gccaggcgct gatcgacggc gacaccgacg ttgtggccgc gaccgaggca
     1801 cggaccgtgg ccgagatcaa cctgcagaac tcgccggctg gggcgagcgc tgtgatcgct
     1861 cccgacgcgg cgcgcatcta cgccgacggc catgccggcg atccggccta cctggtcagc
     1921 ggcatcctga tcagcgggac gacggtccgc gtcgcgatct cctccacgct gggtacgacg
     1981 ctggcccgcg tggtgggcgt gaccaccatg gaggctggtg cgcggtcgcg ctgccagctg
     2041 cagggtggcc ccgccatccc gatcgtggcc cgccgctacg agaacgcccc ggggccgagc
     2101 aacggcttcc gcgacttcct ggccaccgag gcaaccagca ccatcgggca ggtggatgcc
     2161 tggaacgtcc tgggctacga cggccggaca ccggccagcg aggtcgagcg tggcccggag
     2221 ttcgagctgt acggcccgaa cgccaaggcg tccaacgaca gctcgttccg cggcttcgtg
     2281 gccctcgacg tccgcaactt ccaggacctg acctcacgcg tctactacaa cggggtggtc
     2341 cccggcgtct ccgagaacac gctcaagtcg aaggagggcg cctacctgct gagcggctac
     2401 cccggccccg ggttcccacc gatcagccag ccggctgatc cgaacgacca ggtcgccacc
     2461 ctgagcggca acgacacggc catggtggtc ggcaacttcc gagacgtgta cgaggttggc
     2521 gatcgcatcc tgctggccgt ctacaacggc acggtgatgc agatcccgga cttcgcgata
     2581 tcgccaccct cggtgatcat tgttccctcc accacaacga cgccagtcgc cggcccgaac
     2641 ttcagcgtca cccgtaacga cgcgttcaac agcacggtca cgcttcactt gcacggcgac
     2701 gcggaggcca cggcccaggg ccaccccgag tgggatctgg tccccactcc ggcgatcacg
     2761 cctccagcgg cgggcgacat gaacgagccg acctggtcca ccaacgtctt cattcccaac
     2821 aagaacggca cgacggtgtc gatgagcggc atccagacca acaacgttcc cgccgggatc
     2881 tacacggtct ggctggaggg ccactccggc gacccgtact tccagacgcg acgcgcgccg
     2941 gtgcccgtgc gggtcggcgg cgccacccgc gacttcagcc tggtgaacag cacgaccagc
     3001 gcgtcgatcg catcggtcgg tggctcggcc gtgctgccca tcttcgtctc gaccaccaat
     3061 gccagcagca cgaagtggaa caccaccaac ccggtcacgc tctccgtcga ccagagctcc
     3121 ttcaccgatt gctccctggt cccgaagacg atcggcgccg gacagctgac cctcagccag
     3181 agcagcctgg tgccgacctc ctcaggtgat ggtgcgcagt cgaacctgac gatcagctcc
     3241 gccggcctcg cgccgggctg ctatcggttc aacctgcgcg cgtacggcac caacggcgac
     3301 ggccagccgg tcgtccacct gcacccggtg actttcacgg tggccaccac ctcgtcggac
     3361 ggcacctacg tggacatcat cggtttcgcc gtcttcgaga tcaccacgct cgacgccaac
     3421 tcgatcgccg gacgagcggt cagcggcgtc tacgccgacc ccaacgacgt ggcactgcgc
     3481 cgagcgcagc aagcccgact catgccctgg taacccccta ggagacctct tcgtggaact
     3541 cgagtacacc gacaagaaca gccgtcgttc gaagctctac atcgccgtgg ggctggtcat
     3601 ggccctggcc gtggccggca tggtctacgc ggcgctgcag ttcagcggca tcaccaagga
     3661 caatgacgtg gtcatgcgcc aggtggtcgt cgcggcgcgg gatatcgcca gtcgcaagcc
     3721 catcgaggag ggcgacgtga ccatgaccac cgtggtggcc aacgagacca acgcctcggc
     3781 cttcacccgc atcgacgagg tgctcggccg catctcgggc gtgcccatcc tgaccaacca
     3841 gatgctcacc cagaacatgc tggcatcgac caccgagggc cagagctaca ccatcctgga
     3901 gctgggcgag gagttcgatc ccaacgggcc ggacctccgc gcggtcagca tctcggtggc
     3961 cgatgaccgc gccgtggcgg gcacgctggt gcccgggcag gtcgtggacg tgatcgtcac
     4021 catggcgatc aacccggagg tcggccagac gcccgaggag gcggaggtga ccaccgccca
     4081 gttcatcccc ggaccgtcga ccaaggtcac cttccaggcg ctcaccatcc tggcgcgcag
     4141 tggtgggctc tacatcatgc gcaccgacct ggcgaccgcc gagcagatcg cggaggtcac
     4201 cgcggcgggg ggccagttca cgctggcact gcggccggac gtggacgatc gcacggcgga
     4261 taccgagggc tcgaccatcg accgcctgat cgaggagttc gagttcccgg tcccgctgcc
     4321 gccggacttc gaggccgagg ccgcagggcg ctaacccccg ctagggcgca tcggcgccca
     4381 ccagggcgcg gtaggccgtc tccacctgcc gcctcgtctc ttccagggcc gctgacccgt
     4441 cgatgatgac gtcggcggcc ctgacctttt cctcctgcga cgactggctg gcgaggcggg
     4501 cccgggcctg ctcgtcggtc atcccgcgag tggccatcac ccgctgaacg gcgtcggcct
     4561 cgtcacagcg cacgacccag gtctgatcgc agcggtcgct gagcggcgac tccagcagct
     4621 tgatggcctc gatgcaggcg gcctccaccc ctgccgcctc cagctcggca aggcgggccc
     4681 gggtctcgtt gcgcaccagc gggtgaagga tgagctcgag gtcgtgaagg gcctccggat
     4741 cggaaaagac gaccgatgcc agctggccgg cgtcgatggt cccgaaccgc gcctcgatcc
     4801 ggcgccgcgc ctccgcgtcg ttgttgcgga tctggcgggc caccgcgtcc aggtcgatca
     4861 ccgccacccc aatctcgctc aacatggcgg ccaccgtgct cttcccgcag ccgatgttgc
     4921 ccgtgagacc gatgaccttc acccgtcctc ccccgccggc tccggctggc agcggggaca
     4981 gaaatgcgtg gatcgctgcc cgaccacgat gcgctggatc ggggtgccgc agcgcaggca
     5041 tggctggccg gtgcgccggt agacgtacag gcgctccgcg ttgtcgcccg gctcagcgtc
     5101 ggtatccacg tagtcgctga agctggcgcc acgattctcg atcccctgcc gcaggaccgc
     5161 tcgcacggcg cggtgcaggc gccgcacctc cgccggcgtc agcgtgtcgg cccggcgcag
     5221 cgggtggagc cgggcgcgcc acagcgcctc gtccgcgtag atgttgccca cccccgcgac
     5281 gaaagactgg tcgagcagca gcgacttgag ccgcgcggac cgcccgcgca gccgctcggc
     5341 gaaacgcgcc gcgctgaagc cgctggcgag gggctccggc ccgtggcggc caaaaacgtc
     5401 gccgacccgg aacgggaggc gcgtcctgcc ctgccgggtc ttgcggggcc gcttcgtgcg
     5461 cgctcgcagg ccgccgccct cccacagccc gatccgcccg aacttccgca cgtcgcggta
     5521 gcgcagctcg cggccgtcgg ccaggcggaa gacgacccgc gcgtacgggt ctgcgtcggc
     5581 gccggcctgc gccaggatca gggcgccggt catgcgcagg gccacggtca tcacccggcc
     5641 atcggcgaga tggatcagca cgctcttcgc ccggcgcccg acgcggctga tctcggcgcc
     5701 gcggaccatc gccacaaact cgtccggctc cgggttggtg atggtgcgtc cccagtgcac
     5761 ctccgcgtcc gtgatgcggg cgccggccac ccggcgctgg aggtcacgcg ccacggtctc
     5821 gacttcggga agctccggca cgagcagtag tgtgcccgat cggaccggct acgggaccag
     5881 ggcgccggcg gcgacctcct cctcggcctc ctcgcgcgcc acctgggcgg cgctcttcgg
     5941 gacgcgccgc cagccgccgg cgtcgccgcg aacgtatcgg tccatgttct cccagtcggt
     6001 gcccaccttg aggtccacct ccagcggcgg gtcgagcggc agcgctgcct ccatgacctg
     6061 ggtcgccagc tcggccagcg cgggaagctc gtcctcatcg gtctcgaaca cgagttcgtc
     6121 gtggacctgg agcagcatcc gggagcgcag gccacgttcg cgcatggcgg cgtccagccg
     6181 caccatggcg atcttcatgc cgtcggccgc agtgccctgg atgggcatat tgatggccat
     6241 ccgctccccc gccgagcgca gcgccgagtt gcgggctgac agctccggca ggtagcggcg
     6301 acggccgagg agggtggtca cgaagccctg gtcgcgcgcc agcatccgga tctcgacggt
     6361 gtagcgccgg attcccgggt aggcggcgaa gtagtcggcg atgaagcgct gggcgtcctc
     6421 gcgcgggatc ttcagccggt cggccagccc gaagtcgctg agcccgtacg cgatgccgta
     6481 gttgatcatc ttggcgatgc tccgctcccg cggcccgacc ttgtcggcgg ggaggcccag
     6541 cacgcgagcg gccgccgcca ggtggatgtc ctcgtgcgcc gcgaagtccg tcttcaaccc
     6601 ctcgtcgccg gagacgtggg ccaggatgcg cagctcctgc tgcgagtagt ccgcacccag
     6661 cagcagcttg cccggccctg ccacgaaggc gctccggatg cggcggccga gcgccgtgcg
     6721 gatggggatg ttctgcaggt tgggatccac gctcgacagc ctgccggtgg cggccaccgc
     6781 ctgctggtag gtggtgtgga gccggccgtc cgggccgacc agggtcggga gcgcgtcgac
     6841 gtaggtgccc ttcagcttcg agatctggcg gtgctcgagg atcaggccaa ccacctcgtg
     6901 ctgctcccgc agctcctcca ggaccgatgc gtccgtcgag aacccggtcc gcgtgcgctt
     6961 cgtcgccggc aggcccagct catggaagag gacctgctcc agctgcttgg gagaaccgat
     7021 attgaacggc cgccccacca gctcgtagat ccgcgcctcc agcccggcca gctgggcgcc
     7081 gaactcggcg ctcataccgg ccagcgcctc gcgatcgatg accaccccgg ccagctcgac
     7141 gcgcgccagc accgggagga gcggcatctc cacctcgcgg tagaggtcca gcaggccgcc
     7201 gtcccgcagc tcggcctcca gctgggggcg gaccaggagg gcggtcagcg cctcggctgc
     7261 agcccggcgc gccgccacct gctcctccgc gccggtctcg gcgatgaccg gccgctccgg
     7321 cagcgtggcg ccgaaacggt tggcggccag gtcctccagg gtctgcgccc ggagcgaggg
     7381 gttgaccatg tagctggcca ccagggtgtc gtccgcggga ccggccacct cggcgccgcg
     7441 cacgg
//
LOCUS       DATACA010000122         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2105386, whole genome shotgun sequence.
ACCESSION   DATACA010000122 DATACA010000000
VERSION     DATACA010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105386"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VHK28_02555"
     CDS             <1..481
                     /locus_tag="VHK28_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2141145.1"
                     /translation="YARRVFVFIRGATNSGMSQYLIDQMAAIENIEVRLSCQISEVRG
                     DTHLSGVTTRGPQGEERLELAAMFIFIGQQPRTDWLEGMVQRDSGGFVVTGPALLEDG
                     KPPLGWGLNREPFLLEASVPGIFAAGDVRHRSIKRIASAVGEGAMAVQFVHQYLATP"
     gene            490..1845
                     /locus_tag="VHK28_02560"
     CDS             490..1845
                     /locus_tag="VHK28_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2141146.1"
                     /translation="MTIEALRAAPLFTGLADDDLARLAATVERMRLAPGEYLVREGDA
                     GDAMFVVVSGELEVTKRAGAAEQPVAVVGPGAIQGEIGAISGASRAASVRAMTDVEVL
                     RVPREGLLALLDAGPEPALALLRTVLERIRGLEMFAREREKLAALGTLAAGLAHELNN
                     PAAAIRRSVSALGQAIAERDRLHPPHELLGVHPDASRAPTDALGRADAIDEVLELVDD
                     SEIAAALVDAGWDAEQLRQAFEGMEPDVARESAGWLAATARIDALLSEVGMAGERISE
                     IVGAVKSYAFLDQAPVQRVNVTAGLDNTLVILRHKLRGTIDVTRHYASDLPEIEAYGS
                     ELNQVWTNLVDNAVDAMDGPGAIDIYADPAPDGGVRVRICDTGPGIPPEVQQRLFEPF
                     FTTKAPGVGTGLGLHIAYSVIARQGGRIEIESTVGEGTCFVVTLPPKPPGSGSERGRE
                     G"
     gene            complement(2034..>2327)
                     /locus_tag="VHK28_02565"
     CDS             complement(2034..>2327)
                     /locus_tag="VHK28_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141147.1"
                     /translation="RNARRTDGETHGFDQEPVEAGERDTTQQRQHEQEGDPPIRDPAS
                     PDADAEDSLATRPDALYGQSEDQAGTPDAGMPTPGGESQGAGDPFRPRQDEDR"
BASE COUNT          308 a          812 c          855 g          352 t
ORIGIN      
        1 ctacgcgcgc cgcgtgttcg tcttcatccg cggggccacc aactcgggga tgagccagta
       61 cctgatcgac cagatggcgg ccatcgagaa catcgaggtt cggctcagct gccagatcag
      121 cgaggtgcgc ggcgataccc acctgtccgg cgtcacgacc cgtggtccgc agggcgagga
      181 gcgcctggag ctggccgcga tgtttatctt catcggccag cagccgcgca ccgactggct
      241 ggagggcatg gtgcagcgcg actccggcgg cttcgtagtg accgggccgg cgctgctgga
      301 ggacggcaag ccgccgttgg gatggggcct gaaccgcgag ccgttcctcc tggaggccag
      361 cgtgccgggg atcttcgccg ccggcgacgt ccggcatcgg tccatcaagc gcatcgccag
      421 tgcggtgggc gagggtgcca tggccgtcca gttcgtgcac cagtatttgg ccacgcccta
      481 ggtgcaccga tgaccatcga ggccctgcgc gcagccccgc tcttcaccgg cctggccgat
      541 gacgacctcg cgcgcctggc ggccaccgtc gagcgcatgc gcctggctcc cggcgagtac
      601 ctggtacggg agggcgacgc cggcgacgcc atgttcgtgg tggtctccgg cgagctggag
      661 gtgaccaagc gcgcgggagc cgccgagcag cccgtggccg tggttggccc cggcgccatc
      721 cagggcgaga tcggcgccat ctccggggcg tcgcgtgccg cttcggtgcg agcgatgacc
      781 gacgtggagg tcctgcgcgt gccccgcgag ggcctgctgg cactgctcga cgcagggccg
      841 gagccggccc tggccctgct gcgcacggtg ctcgagcgaa tccgtggcct ggagatgttc
      901 gcccgcgagc gggagaagct ggccgcgctg ggcacgcttg ccgccgggct cgcccacgag
      961 ctcaacaacc cggcggccgc catccgccga tccgtctccg cgctggggca ggccatcgcc
     1021 gagcgggacc gactgcaccc gccgcacgag ctgctcggcg tccatcccga cgcgagccgg
     1081 gcgcccaccg acgccctggg ccgcgcggat gccatcgacg aggtcctcga gctggtggac
     1141 gactcggaga tcgccgccgc cctggtcgac gccgggtggg atgcggagca gctgcgccag
     1201 gccttcgagg gaatggagcc ggacgtggcc cgcgagagcg ccggctggct ggctgccacc
     1261 gcccggatcg acgctttgct gtccgaggtt ggcatggcgg gggagcggat cagcgagatc
     1321 gttggcgccg tgaagagcta cgccttcctg gaccaggcac ccgtgcagcg ggtcaacgtc
     1381 acggccgggc tggacaacac gctcgtgatc ctgcgtcaca agctgcgcgg cacgatcgac
     1441 gtgacgcgcc actacgcgtc ggacctgccg gagatcgagg cctacggcag cgagctgaac
     1501 caggtgtgga ccaacctggt cgacaacgcc gtcgacgcca tggatggccc cggagcgatc
     1561 gacatctacg ccgatccggc gcccgacggt ggcgtgcggg tgcgcatctg cgataccggc
     1621 cccgggatcc cgccggaggt ccagcagcgc ctgttcgagc cgttcttcac caccaaggct
     1681 ccgggcgtgg gtaccggcct cgggctgcac atcgcctact cggtgatcgc ccgccagggc
     1741 ggtcggatcg agatcgagtc cacggtgggc gaggggacgt gcttcgtggt caccctgccg
     1801 cccaagcccc cgggaagcgg atcggagcgg ggtcgagagg ggtaggcggc gtccctcgtt
     1861 ggaccgctgc gctaaccccg gccggggtca tggggtggct gcaacggcca tcgatccggc
     1921 cacctgcagc cttcggtcgc cgcgggggtc atccggtggc tgccgcggcc agcgagaggc
     1981 ccattccgct ggctgcttcg ccgttggagc catcgcgtgg ctacgcgcca ggttcagcgg
     2041 tcctcatcct ggcggggccg gaagggatcc cccgcgccct ggctctcgcc gcccggggtc
     2101 ggcattccgg catccggcgt cccggcctgg tcctcggatt ggccgtagag cgcatccggc
     2161 ctggtagcca gggagtcctc ggcatccgcg tccggtgaag cgggatcacg aatgggaggg
     2221 tctccctcct gctcgtgctg ccgctgctgg gtcgtgtcgc gctcgccggc ctccacgggt
     2281 tcctggtcga agccgtgggt ctcgccgtct gtgcgcctgg cgttgcg
//
LOCUS       DATACA010000123         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2667882, whole genome shotgun sequence.
ACCESSION   DATACA010000123 DATACA010000000
VERSION     DATACA010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2667882"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1381
                     /locus_tag="VHK28_02570"
     CDS             56..1381
                     /locus_tag="VHK28_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX2141148.1"
                     /translation="MTSRQLTMMGLGSAIGAGLFLGSGAGIQAAGPAVLISYLLAGTL
                     VIIVMWALGEMAAAHPSSGAFSTYAEKAMGRTAGATIGWLWWLQLVTVIAAEAVGAAA
                     LLASVWPVLPSWLLALVFMVVFTGINLMGVKNYGEFEFWFAIIKVAAIVAFLALGAAL
                     IFGWLPHAPSPGLSNLVGGDGFAPAGLAGIAAGLLVVVFAFGGTEIVAVAAAETNDPQ
                     HSVSRAIKTVVWRILVFYFGSVLVIATVVPWDSEALASPFAAVLSVAAIPGADVAITI
                     IAAFALLSALNANLYGASRMIFSLSQRGEAPALLQQISLRRVPVAAVLSSVAFGFVAT
                     VMELLFPEKVLPVLLSFVGSTCLVVWGMVLVSQLILRRRADRAGQDLPMRMRGFPAIT
                     YVGLGLLTAILAVGFFSPGTSAQLVSTAALITGIAVLNKLFARRGSAVR"
BASE COUNT          208 a          527 c          480 g          322 t
ORIGIN      
        1 gctgcactcc ggcgccaatg aagccggcgc tccccgccac ggtctcggcc agtcaatgac
       61 gtcgcggcag ctcaccatga tgggactggg cagcgcgatc ggtgccggac ttttcctcgg
      121 ctccggcgca ggcatccagg cggcaggacc ggcagtgctg atctcctacc tgctcgccgg
      181 gacgctggtc atcatcgtga tgtgggcgct gggcgaaatg gccgcggcac atcccagcag
      241 cggcgcgttc tccacctatg cagaaaaagc catgggccgg acggccgggg caaccatcgg
      301 ctggttgtgg tggctccagc tggtgaccgt catcgcggcc gaggccgtcg gcgcggctgc
      361 cctgctcgcc tccgtctggc ccgtcctgcc gtcatggctg ctggccctgg ttttcatggt
      421 cgttttcacc ggcatcaacc tgatgggcgt caagaactac ggtgagttcg agttctggtt
      481 cgccatcatc aaggtcgcgg ccatcgtggc gttcctggcc ctcggcgcgg ccctgatctt
      541 cggctggctc ccccacgcac cctcgcccgg actgtccaac ctcgtaggcg gcgacggctt
      601 tgctcccgcc ggcctggccg gcattgccgc gggcctgctg gtggtggtgt tcgcctttgg
      661 cggcaccgaa atcgtcgcgg tggcagctgc ggaaacgaac gacccgcagc acagtgtctc
      721 ccgggccatc aagacggtgg tgtggcggat tctggtcttc tacttcggct cggtactggt
      781 catcgccacg gtggtcccgt gggattccga agccctcgcc tcccccttcg ccgcggtgct
      841 gtccgtcgcc gcgataccgg gcgcggatgt ggccatcacg atcatcgccg cctttgccct
      901 gctctcggcc ctgaacgcca acctgtacgg tgcctcccgg atgatcttct ccctctccca
      961 gcggggcgag gcaccggcgc ttctccagca gataagcttg cgccgcgttc ccgtcgcggc
     1021 agtcctgtcc tcggtggcct tcgggttcgt ggcgaccgtc atggaactgt tgttcccgga
     1081 gaaggtcctg cccgtgctgc tgagcttcgt cgggtcgacc tgcctggttg tctggggcat
     1141 ggttctggta tcgcagttga tcctgcgccg ccgtgcggac cgcgcgggcc aggacctgcc
     1201 gatgagaatg cggggctttc cggccatcac ctacgtgggc ctgggtctgc tgaccgccat
     1261 cctggccgtc ggattctttt ctccgggcac cagcgcccag ctggtttcca cagcagcatt
     1321 gatcacgggc atcgccgttc tgaacaagct ctttgcacgc cggggttcgg cagtccgcta
     1381 accaacccag ggaaaccacc tagcaaattg ctactcccgg acccagcatc gttgcgttaa
     1441 ctgtgcacac tgcacagtag ttttcttcca tattgcacac cccgcttgac cgtgactatg
     1501 gtcacttaca ggacggccgc cgggccgctt gcggttt
//
LOCUS       DATACA010000124         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2747975, whole genome shotgun sequence.
ACCESSION   DATACA010000124 DATACA010000000
VERSION     DATACA010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747975"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /gene="meaB"
                     /locus_tag="VHK28_02575"
     CDS             complement(<1..620)
                     /gene="meaB"
                     /locus_tag="VHK28_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX2141149.1"
                     /translation="MPEPAFAELAAGDRRPLARLLTRIESGDPTLRPQLPELFAAGGG
                     AHVVGITGPPGSGKSTLVSALTGEWRRRGRRVGVLAVDPSSPYTGGAILGDRIRMMEH
                     AADRDVFMRSMASRGELGGLASTTWIAAAALDAAGFDPVVIETVGAGQSEVEIARLAE
                     TTVVVEVPEMGDEVQAIKAGLLEVADVIAVNKGDRPGADRAARQLRA"
     gene            complement(620..1051)
                     /locus_tag="VHK28_02580"
     CDS             complement(620..1051)
                     /locus_tag="VHK28_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEX2141150.1"
                     /translation="MTNQTDANAAERPPIRVLIAKPGLDGHDRGAKVLARGLRDEGFE
                     VIYTGLRQTPEMIADAAEQEDVDVVGLSILSGAHMALLPRVVELVRERGMDDVLVAAG
                     GIIPDADIAKLKKAGVAEIFGPGTSIGQIARYFREHARRRA"
     gene            1102..1851
                     /locus_tag="VHK28_02585"
     CDS             1102..1851
                     /locus_tag="VHK28_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2141151.1"
                     /translation="MRLRIATLNILGTADRWEERRPLLVEGIAELGPDVLGLQEVEFS
                     ADQDELLASAMAPDSTVQRATEHGTFGNSLVVGPVLRGAWNGPPRGTELALPGDRAAA
                     IVDVPLDDGPLRLVTTHLHWVPEEQELRLEQLMRLVEHLAALEPAAATVLTGDLNATP
                     DEPAITLLRTAGFSSAFAAIHGADPEWTYPSPMTPADVAVRPPSPIDYVWVLGSAQVV
                     DASTALDRPSPHDPSLYPSDHRAVVADLEVP"
     gene            complement(1922..2221)
                     /locus_tag="VHK28_02590"
     CDS             complement(1922..2221)
                     /locus_tag="VHK28_02590"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX2141152.1"
                     /translation="MPYAPLLTEPEIAAEMDRLPEWTREGEWISRTVRCASFREAIEL
                     VNRVADAAEVADHHPDITIVWRRVTFRLTSKASGGITARDVDMAQRIEAAAAPRD"
BASE COUNT          335 a          857 c          816 g          342 t
ORIGIN      
        1 gcgcgcagct gacgagccgc gcggtcggcg ccgggacggt cacccttgtt gacggcgatc
       61 acgtcggcaa cctcgagcag gccggccttg atggcctgga cctcgtcgcc catctcgggg
      121 acctccacga ccaccgtggt ctcggccagg cgggcgatct cgacctccga ctgcccggcg
      181 ccgaccgtct cgatcaccac ggggtcgaag cccgccgcgt caagcgccgc ggcggcgatc
      241 caggtggtgg acgccaggcc acccagctcg ccgcgcgacg ccatggagcg catgaacacg
      301 tcacggtcgg cggcgtgctc catcatcctg atgcgatcgc ccaggatggc gccgcctgtg
      361 tagggcgacg agggatccac ggccagcacg ccgacccgcc ggccgcgacg tcgccactcc
      421 ccggtgaggg cgctgaccag cgtggacttg ccgctgcccg gcggcccggt gatcccaacc
      481 acgtgcgcgc cgcccccggc cgcgaagagc tccggcagct gggggcgcag ggtcgggtca
      541 ccgctctcga tgcgggtcag cagccgggcg agcggtcgcc ggtccccggc ggccagctcc
      601 gcgaaggccg gctcgggcat caggcgcgac ggcgcgcgtg ctctcggaag taccgcgcta
      661 tctgtccgat gctggtcccc gggccgaaga tctctgcgac gcccgccttc ttgagcttcg
      721 cgatgtcggc atcgggaatg atcccgccgg ccgccaccag cacgtcgtcc atgccccgct
      781 cacggacgag ctccaccacc cgcggcaaca gggccatgtg ggcgccggac aggatggaga
      841 ggccgaccac gtcaacatcc tcctgctccg cggcatccgc gatcatctcc ggggtctggc
      901 gcaggccggt atagatcacc tcgaagccct cgtcgcgcag cccgcgggcc agcaccttgg
      961 ccccgcgatc gtggccatcg agacccggct tggcgatcag gacgcggatg ggcgggcgct
     1021 cggccgcgtt cgcatcggtc tggttggtca tcagcgcgcc gattctagcc cggcacgatt
     1081 ctggccatcg gtcgatcggc catgcgcctc cgaatcgcca ccctgaacat cctgggaacc
     1141 gccgaccgct gggaggagcg acggcccctg ctggtcgagg gaatcgcgga actcgggccg
     1201 gacgtgctgg ggctgcagga ggtcgagttc tcggccgacc aggacgagct gctggcttcc
     1261 gccatggcgc ccgattcgac cgtccagcgc gcgaccgagc atggcacctt cggcaacagc
     1321 ctggtggttg ggcccgtcct gcgcggcgcc tggaacgggc cgccgcgcgg caccgagctg
     1381 gcgctgccgg gagaccgggc ggccgccatc gtcgacgtcc cgctggatga cggcccgctt
     1441 cgattggtca ccacccatct gcactgggtg ccggaagagc aggagctgcg gctggagcag
     1501 ctgatgcgcc tggtcgagca cctggccgcg ctggagccgg cagccgccac ggtccttacc
     1561 ggagacctga acgcgacgcc ggacgagccg gccatcaccc tgctgcgcac tgccggcttc
     1621 agctcggcgt tcgccgccat tcacggcgcg gaccctgaat ggacctaccc ctcgccgatg
     1681 acccccgcgg acgtggccgt ccgcccgccc tcacccatcg attacgtgtg ggtgttgggc
     1741 agcgctcagg tggtggatgc cagcaccgcc ctcgatcggc cgtccccgca cgacccgagc
     1801 ctctacccgt ccgatcaccg tgcggtggtc gccgacctgg aagtgcccta ggagccgctg
     1861 gatcggcctc atgcggcggc cgtcggacgc cggttggaat tcgcctcacg aataggtggc
     1921 gtcaatcacg gggcgcggcg gcggcttcga tccgctgagc catgtccacg tctcgcgcgg
     1981 tgatcccgcc cgaggccttg ctggtgaggc ggaaggtgac ccgccgccag acgatggtga
     2041 tgtcggggtg gtgatcggcg acctctgcgg catccgccac ccggttgacc agctcgatcg
     2101 cctcccggaa tgatgcgcag cgcacggtac ggctgatcca ttcgccctcg cgggtccact
     2161 ccggcaggcg gtccatttcc gcggcgatct ccggctcggt cagcaggggc gcgtacggca
     2221 tggaccgatg atatccgcac accagcgggc ctgggcctgc ggctagcggc ctgacgagcg
     2281 accgatcagg gtcaggaact cggagcgcgt ccggccatca cgacggaagg tgccgagcat
     2341 ggagctggtg
//
LOCUS       DATACA010000125         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_178930, whole genome shotgun sequence.
ACCESSION   DATACA010000125 DATACA010000000
VERSION     DATACA010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178930"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..719)
                     /locus_tag="VHK28_02595"
     CDS             complement(30..719)
                     /locus_tag="VHK28_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX2141153.1"
                     /translation="MADEVSTTVRSMVERVLEADVRDQMARRGRELATVVAEASDTVS
                     ARAAEAWRESAPTRRDAQRQVRKASRDAMKWGRRTWKKDLGPSVKQLWSRRAAAIGAA
                     GAALPVAGELVDDAAVRLGIRRREERRWTAFFLGVVLGAVAGAVVALMTAPKPGREMR
                     DELAERARDAASRAREAGTEWKPLFQRETNGAPLVAPAPADPAVSEPLGPEASDVPID
                     SLSPEGEEPLH"
     gene            956..2104
                     /locus_tag="VHK28_02600"
     CDS             956..2104
                     /locus_tag="VHK28_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141154.1"
                     /translation="MPAFAADPGFQTIGQEAARTAILRAFRGGRLGRTLLVHGPAGAG
                     KTAFVDDLLALLVCERPDPELGPCNACRFCRQARERSHPDLVSASPEVWREARSSGES
                     IVAAARRWLLEASGAPIAARHRVVLIEGADRANEQTQNALLKSLEEPSPRQLFVLVAD
                     EPGRLLPTIRSRCQPLRIGPVAHAELVAWLIEHERLPADQAEPIARMAGGLAGRAVAL
                     AGRAPLVEWRRRTQQELLDLLQRGRAERLGSARELIDSALRVTSAAAGLEPLVEAGSG
                     SQAATAAGAEQRAAALLVVDAWLGLTRDLLLTAAQRAELAPAAALLGDVQEPARRAGI
                     GPLVAFVDVLERVREGLRQNASPRLALEVAMLAWPGTDAQPLPATAAR"
     gene            2101..2388
                     /locus_tag="VHK28_02605"
     CDS             2101..2388
                     /locus_tag="VHK28_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX2141155.1"
                     /translation="MTDHQRMTARISGRVQGVGFRWWVRSRAAELGLTGWVANDPDER
                     TVSILAEGTPEMLDRFEGMLWQGPPSARVERVEAERQPASGQFGGFQISRP"
     gene            2385..>2619
                     /locus_tag="VHK28_02610"
     CDS             2385..>2619
                     /locus_tag="VHK28_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141156.1"
                     /translation="MNWRAAGCLLVGVAGFVGLGLLAMSMTFNDPGGCPDTLQWDDRT
                     YVASGAPASSPSLAEPGDPSEIGSTFFGLTTRRV"
BASE COUNT          299 a          993 c          954 g          373 t
ORIGIN      
        1 gccgcccctt cttgcgtcgt ggagtgggtc tagtggagcg gctcctcgcc ctccggcgat
       61 agcgagtcga tcggcacgtc gctcgcctcg ggtcccagcg gctccgagac ggccggatcg
      121 gcgggggccg gcgccacgag cggtgcgccg ttcgtctcgc gctgaaagag cggcttccac
      181 tctgtgccag cctcgcgggc tcggctcgcg gcgtcgcggg cacgctcggc gagctcgtcg
      241 cgcatctcgc gtccgggctt gggcgcggtc atcagcgcca ccaccgcgcc agccaccgct
      301 cccaggacca cgcccaggaa gaaggccgtc cagcggcgct cctcccgccg gcgaatcccg
      361 aggcgcaccg cggcgtcgtc caccagctcg ccggccacgg ggagtgctgc cccggccgcg
      421 ccgatggccg ccgcccggcg gctccacagc tgcttcacgg aaggcccgag gtccttcttc
      481 caggttcggc gcccccactt catcgcgtcg cggctcgcct tgcggacctg gcgctgcgcg
      541 tcgcggcggg tgggtgcgga ctcgcgccac gcctcggccg cacgcgctga gacggtgtcg
      601 ctggcctcgg cgaccacggt cgccagctcg cgaccgcgtc gcgccatctg gtccctcacg
      661 tccgcctcga gcaccctctc aaccatcgag cggaccgtgg tcgagacctc atcggccatg
      721 tcgtcatcct cctgttcgcg ggcagcgggc cggtgccaga agtatgccac cggcggagcg
      781 gcatgaccat cgggcgccca ggcgggacgc ctctcgaagg cgggtccggt tgccggccgg
      841 ccgccagcgc gatgtgcccc gggcgtcccg ccgggcacgg accgccagca tgccgcagga
      901 cacctcaccg gcggcttacc ggggccttat cgccgccctt gcctatactc gcgccatgcc
      961 cgcgttcgcg gccgatcccg gcttccagac catcggtcag gaggccgcca ggacggccat
     1021 cctccgcgcc ttccgcggcg gccggctggg ccgcacgctc ctggtccacg ggcccgccgg
     1081 ggcgggcaag acggccttcg tggacgacct cctggcactg ctcgtctgtg agcggccgga
     1141 ccccgagctg ggcccctgca atgcctgccg cttctgccgc caggcgcgcg agcgctcgca
     1201 cccggacctg gtcagcgcat cgccggaggt gtggcgcgag gcgcgctcca gcggcgagag
     1261 catcgtcgct gcggcgcggc gctggctcct ggaggcgtcc ggtgccccga tcgccgcccg
     1321 ccaccgggtt gtgctgatcg agggggcgga tcgcgccaac gagcagaccc agaacgccct
     1381 cctcaagtcg ctggaggagc cgtctccgcg gcagctgttc gtgctggtgg cggacgagcc
     1441 gggacggctg ctgccgacca tccgctcgcg ctgccagccg cttcgcatcg gtccggtggc
     1501 gcatgccgag ctggtggcct ggctcatcga gcacgagcgg ctgcccgccg accaggcgga
     1561 gccgatcgcc cgcatggccg gtggcctcgc cggccgggcc gtggccctcg ccggccgggc
     1621 gccgctggtc gagtggcgcc ggaggacgca gcaggagctg ctggacctgc tgcagcgcgg
     1681 ccgtgccgag cggctgggca gcgcgcggga gctgatcgac agcgcgctgc gcgtcacctc
     1741 ggctgcggcc ggcctggagc cgctggtcga ggccgggtcg ggatcacagg cggccaccgc
     1801 cgccggcgcc gagcagcgcg cggcggcgct gctggtcgtc gatgcctggc tcgggctcac
     1861 acgggatctc cttctgaccg cggcgcagcg tgccgagctg gcgccagcgg ccgccctgct
     1921 cggagacgtg caggagccag cgcgccgcgc cggcatcggt ccattggttg ccttcgtgga
     1981 tgtcctggag cgcgtgcgcg aagggctccg ccagaacgcg tccccgcgcc tggccctgga
     2041 ggtcgccatg ctggcctggc cggggacaga tgcccagccg cttccggcca ccgcggcgcg
     2101 atgaccgatc accagcgaat gaccgcacgg atctccgggc gggtgcaggg ggtcgggttc
     2161 cgatggtggg tccgctcccg ggcggccgag ctcggactga ccggctgggt ggccaacgat
     2221 cccgatgagc gcaccgtctc catccttgcc gaagggaccc ccgagatgct ggaccgattc
     2281 gagggcatgc tctggcaggg accgccgtcc gcccgtgtcg agcgcgtcga ggcggagcgg
     2341 cagcccgcct ccgggcagtt cggcggcttc cagatctcgc gcccgtgaac tggcgtgcgg
     2401 cggggtgcct gctggtgggc gtggcggggt tcgtcggcct cggcctgctg gccatgtcca
     2461 tgaccttcaa cgatcccggc ggctgccccg acacgctgca gtgggacgac cgcacctacg
     2521 tcgcttcggg agcgccggca tcatcgccgt cgctcgccga gcccggcgac ccgtcggaga
     2581 tcggctccac cttcttcgga ttgaccacgc gacgcgtct
//
LOCUS       DATACA010000126         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_258495, whole genome shotgun sequence.
ACCESSION   DATACA010000126 DATACA010000000
VERSION     DATACA010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258495"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="nadD"
                     /locus_tag="VHK28_02615"
     CDS             complement(<1..199)
                     /gene="nadD"
                     /locus_tag="VHK28_02615"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554817.1"
                     /note="transfers an adenyl group from ATP to NaMN to form
                     nicotinic acid adenine dinucleotide (NaAD) which is then
                     converted to the ubiquitous compound NAD by NAD
                     synthetase; essential enzyme in bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX2141157.1"
                     /translation="MLFAAAEEVSVLPDEELLDGEPRRLGILGGTFDPPHVGHLWLAT
                     LAADVLGLDRVLFMPAAQPPHK"
     gene            complement(211..1512)
                     /gene="obgE"
                     /locus_tag="VHK28_02620"
     CDS             complement(211..1512)
                     /gene="obgE"
                     /locus_tag="VHK28_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX2141158.1"
                     /translation="MFADHARIMVAAGAGGDGASSFRREAHVPRGGPDGGDGGRGGDV
                     VLEAEAGMTTLGEFQRHRHFRAKPGGRGAGKKAHGRNGASVTLHVPPGTVVREVDMEA
                     RAIGPFLGELITPGDRLVVARGGRGGRGNAHFATATHRAPTHYEKGTPGEERWIELEL
                     KLIADIGLVGAPNAGKSTLLAALTAAQPKVASYPFTTITPNLGVIVFEDERSAVIADV
                     PGLIEGASEGHGLGHAFLRHVERTRVLVAVVDGADDDPVAEWQAVAEELRRHDPRLLE
                     RQMPMVVTKLDLPEVRDRWSELRRALHASGHAPIGVSAHDGTGLDELRRLLAAALERA
                     EVASEVEPPGADEMRVHRFDPLDAGWLVVAEDDGVRVRGRRIENIAARTDFENPESRD
                     RFQRTLERMGIDAELRRQGVGAGTLVRIGRVELEWSDEDDG"
BASE COUNT          213 a          590 c          524 g          220 t
ORIGIN      
        1 gcttgtgcgg cggctgggcg gccggcatga acagcacgcg gtccaggccc agcacgtcgg
       61 ccgccagggt cgccagccac aaatgcccga catgaggtgg gtcgaaggtg ccgcccagga
      121 tgccgagacg tctcggttcg ccgtcgagca gctcttcgtc cggaaggacc gatacctcct
      181 cggccgcggc gaagagcatg gccggcccgc tcacccgtcg tcctcgtccg accactccag
      241 ctccacgcgc ccgatgcgca ccagggtgcc cgcccccact ccctggcgac gcagctccgc
      301 gtcgatgccc atccgctcca gcgtgcgctg gaaccggtcc cgcgactcgg ggttctcgaa
      361 atcggtccgc gcggcgatgt tctcgatccg gcggccgcgg acgcgcaccc catcgtcctc
      421 ggctaccacc agccagccgg cgtccagcgg gtcgaaccga tgcacccgca tctcatcggc
      481 cccgggcggc tccacctcgg aggcgacctc ggctcgttcc agggcagcgg ccagcaggcg
      541 gcgcagctcg tccagcccgg ttccgtcatg ggcggagacg ccgatcggcg cgtgaccact
      601 ggcgtgcagc gcgcggcgga gctcgctcca tcggtcccgc acctcgggga ggtccagctt
      661 ggtgaccacc atcggcatct ggcgctccag gagccttggg tcatgccggc gcagctcctc
      721 ggccacggcc tgccactcgg cgaccgggtc gtcgtccgcc ccgtcaacga ctgccaccag
      781 cacccgggtg cgctcgacgt ggcgcaggaa ggcgtggccg agcccgtggc cctccgacgc
      841 gccctcgatc aggccgggaa cgtcggcgat caccgcgctg cgctcgtcct cgaagacgat
      901 cactcccagg ttgggcgtga tggtggtgaa ggggtagctg gccaccttcg gctgggcggc
      961 ggtgagggcg gccagaagcg tcgacttgcc ggcgttggga gcacccacca gaccgatgtc
     1021 ggcaatcagc ttcagctcca gctcgatcca gcgctcctcg cccggcgtcc ccttctcgta
     1081 gtgcgttggt gcacggtggg tggcggtagc gaagtgggcg ttgccacgac cgccacggcc
     1141 gccgcgcgcg accaccagcc ggtcccctgg ggtgatcagc tcgccgagga aggggccgat
     1201 ggcacgcgcc tccatgtcca cctcgcggac cacggtgccc ggtggcacgt gcaaagtgac
     1261 cgatgccccg ttgcggccat gggccttctt gccggccccg cgcccgccgg gcttggcgcg
     1321 gaagtggcgg tggcgctgaa actcgccaag cgtggtcatg ccggcctccg cctccagcac
     1381 aacgtcgccg ccgcggccgc cgtcgccacc gtccggtcct ccacgcggca cgtgtgcctc
     1441 ccgccggaat gaggacgctc catctccgcc agccccggcc gccaccatga tgcgggcgtg
     1501 gtcagcgaac atcggaccga tgatagcaac aggccgtcgc ccggccg
//
LOCUS       DATACA010000127         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2588264, whole genome shotgun sequence.
ACCESSION   DATACA010000127 DATACA010000000
VERSION     DATACA010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588264"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1404)
                     /locus_tag="VHK28_02625"
     CDS             complement(43..1404)
                     /locus_tag="VHK28_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase"
                     /protein_id="HEX2141159.1"
                     /translation="MAWRAALLAVLVLLATGVNAVHGAVAAGPSARARPVGAPDFNTP
                     AAQLRVTLGRVLAEHAFLLGEAMRAGLGEASGFQAAGAALEQNSAELEGLMADVYGHD
                     VASRFGELWRRHVAYLVDYTRALAEDDQAAQDLAEHQLHDYVTEFSAFLASANPNLPV
                     SALERLVGEHVQQLEAIAAFDAGEYAEAYPTVRETYAHMFVIADGLSEAMALQFPERF
                     TGKSVAYSPAGDLRLNLDRLLGEHTMLAGTAMRAGASDADDRAAAEEALAANTDALGA
                     AVTAIYGEEAGAAFVDLWSVHTAAYLEYVASVQSSDVTGRDEALTALGRYRDEFSAFL
                     ADANPQLSAQALREMLGHHTDQLVRQVDAYSAGDHREAYALAREAFSHAFAMGDALAL
                     AIAEQFPDRFPDAATRGGWDTSRMGAILVGFAALAMAGQEVSRRRRRTQAEPWPVMRA
                     SRT"
     gene            complement(1441..>1908)
                     /locus_tag="VHK28_02630"
     CDS             complement(1441..>1908)
                     /locus_tag="VHK28_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141160.1"
                     /translation="AVVVPFPNAVDRSVRLTGSGGSGATLCLPVGSRPWTFASVELHT
                     RSWVGTGVWLRMADEVRGVLVDAEGRFIAEPGGRWITEAPAEPDQWHRVEFVREGDER
                     FSVTIVPLPERGNRGGVPLPPTSEVGERGEVALCIGPPSGPAAVVYVDNIVVE"
BASE COUNT          301 a          685 c          634 g          288 t
ORIGIN      
        1 actagccatc gcctcccaaa atgcttgagg tgcgctggca ggttacgtcc gtgatgcacg
       61 cattacgggc cacggctcgg cctgcgtgcg ccgccgtcgg cgactgacct cctggccggc
      121 cattgccaag gccgcgaaac cgaccaggat ggcacccatc cggctggtgt cccatccgcc
      181 acgagtggcc gcgtcaggga agcggtccgg gaactgctcc gcgatggcca gtgccagggc
      241 gtcgcccatg gcgaaggcat ggctgaatgc ctcgcgcgcg agggcgtacg cctctcggtg
      301 gtcgccggcg gagtatgcat ccacctgccg caccagctgg tcggtgtggt ggccgagcat
      361 ctcgcgcagc gcctgggccg aaagctgcgg attcgcgtca gccaggaagg cgctgaactc
      421 gtcgcggtag cgaccgaggg ccgtcagcgc ctcgtcgcgg ccggtgacgt cggaggactg
      481 gacgctggca acatattcca ggtacgcggc ggtatgcacc gaccacaggt cgacgaaggc
      541 ggcacctgct tcctcgccgt agatcgccgt cacggccgca cccagcgcgt cggtgttcgc
      601 tgccagggcc tcttccgccg ccgcccgatc gtccgcgtca cttgcccccg cccgcatcgc
      661 ggtcccggcc agcatggtgt gctcgcccag caatcggtcc aggttcagcc gcaggtcgcc
      721 agccggactg taggccacgc tcttcccggt gaagcgctcg ggaaattgca gagccatcgc
      781 ctccgacagg ccgtcggcaa tcacgaacat gtgagcgtag gtctcgcgga ccgtcgggta
      841 ggcctcggcg tactcgccgg cgtcgaacgc ggcgatggct tccagctgct ggacgtgctc
      901 gcccaccagc ctctcgagcg cgctgaccgg caggttgggg ttggcgctgg cgaggaaggc
      961 gctgaactca gtcacgtaat cgtgcagctg gtgctccgcc aggtcctgcg cggcctggtc
     1021 gtcctccgcc agggcccgcg tgtagtccac caggtacgcg acgtgacggc gccacagttc
     1081 gccgaaccga ctggcgacgt cgtggccgta gacgtcggcc atcagcccct cgagctcggc
     1141 gctgttctgc tccaacgccg ctccggccgc ctggaagccg ctggcctcac ccaatccggc
     1201 gcgcatcgct tcgcccaaca ggaatgcgtg ctccgcgaga acccgtccga gcgtgacccg
     1261 cagctgcgcc gcgggcgtgt tgaagtccgg cgcgccgacg ggtcgggcgc gagcactcgg
     1321 cccggccgcg accgcgccgt gcacggcgtt cacacccgtc gccagcagga ccaggactgc
     1381 gagtagagca gcgcgccagg ccattaggaa accgtccttt ctgagtatta cgacgcctcg
     1441 tcactcaacc acgatgttgt ccacgtaaac cacggctgcc gggccggatg gagggccgat
     1501 gcagagcgcg acttccccgc gttcccccac ctcggaggtc ggtggaagtg ggacgccgcc
     1561 tcgatttccc cgctcgggca ggggcacgat cgtcaccgaa aaccgctcat cgccctcacg
     1621 cacgaattcc acgcggtgcc attggtccgg ctcggcaggt gcctcggtga tccaccggcc
     1681 accaggctcg gcgatgaatc tgccctcggc atcaaccagc acgccgcgca cttcgtccgc
     1741 catgcgcagc cacacgccgg tgccgaccca ggaccgggtg tgaagttcga cggaggcaaa
     1801 ggtccatggt cgagagccga ccgggaggca gagcgtcgcc ccgctgccgc ccgagcccgt
     1861 cagccgaacg ctgcgatcga cggcgttagg gaacgggacg accacggc
//
LOCUS       DATACA010000128         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2588339, whole genome shotgun sequence.
ACCESSION   DATACA010000128 DATACA010000000
VERSION     DATACA010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588339"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VHK28_02635"
     CDS             <1..589
                     /locus_tag="VHK28_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141161.1"
                     /translation="RTVQDFDLPMGLVVMPMFLFGGVFFRLNYERIYEGMMATPLGIR
                     DIAIGELIWSLFRGTLYAAAFVGVMFTLGLVFSPWVLLVVPGAILLGAAFSAAGLATT
                     SFLRTVQDFDLPMGLVVMPMFLFGGVFFPIAVYPEPLQVLVQALPIYRGVHLFRGLST
                     GALDAWMLFDVAYLLVLTAIGLTIAMRQMERRLIK"
     gene            complement(582..1217)
                     /gene="pgsA"
                     /locus_tag="VHK28_02640"
     CDS             complement(582..1217)
                     /gene="pgsA"
                     /locus_tag="VHK28_02640"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX2141162.1"
                     /translation="MDEAADARPRQASRLTTLPNLLGVARILATPVVVVLMLTAFPGA
                     GLAAFIVFALAAATDSLDGAIARARGQVSPLGIFLDLTADKVLVAGVLVAMVEVGLLP
                     TWMVALLLIRELVVQGVRQVAATADVVIASRAMGKGKTFAMLVVMGLLLLGFDAATGG
                     PLSGLGGVGALLQTTGYWVMVVATALSVASGWDYVRAALPVLLGRPDEPPT"
     gene            complement(1201..>1726)
                     /locus_tag="VHK28_02645"
     CDS             complement(1201..>1726)
                     /locus_tag="VHK28_02645"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX2141163.1"
                     /translation="ATNALRTLGTRWAAAVFALDVAKGMLAVALARMLFVGGPGDADD
                     WVAAGAGLAAVVGHNWSVFIGFAGGRGVATSAGGLLVLTPLTLAIVGPLAAGATWRTR
                     YVSLGSIIAGVSAPIATAALAAAGMAPLAAVAYSLAVGALVVISHADNMSRLRAGTER
                     RLGDKEVVTHGRGG"
BASE COUNT          257 a          632 c          591 g          246 t
ORIGIN      
        1 gcgcaccgtc caggacttcg acctgcccat gggcctggtt gtgatgccca tgttcctgtt
       61 cggcggcgtc ttcttccgcc tcaactacga gcgaatctac gagggcatga tggccacccc
      121 gctcggcatc cgggacatcg ccatcggcga gttgatctgg tcgctgttcc gcgggacgct
      181 gtacgccgcg gccttcgtgg gcgtgatgtt cacgctcggc ctggtgttct caccttgggt
      241 gctgctggtg gtgccgggcg ccatcctgct gggcgccgcc ttcagcgcgg ccgggctggc
      301 gaccacctcc ttcctgcgca ccgtccagga cttcgacctg cccatgggcc tggttgtgat
      361 gcccatgttc ctgttcggcg gcgtcttctt cccgatcgcg gtctaccccg aaccgctgca
      421 ggtcctcgtc caggcactgc ccatctaccg cggcgtgcac ctgttccgcg ggctgtcgac
      481 tggggctctc gatgcctgga tgctgttcga cgtcgcctac ctgctggtgc tgaccgccat
      541 cgggctgacc atcgccatgc ggcagatgga gcggcgactg atcaagtagg tggctcgtcc
      601 gggcggccga gcagcaccgg cagcgcggcg cggacgtaat cccagccgga ggccacgctg
      661 agcgccgtgg ccaccaccat cacccagtag ccggtggtct gcagcagggc tcccacgcca
      721 ccgagcccgg agagcgggcc gccggttgcc gcatcgaaac ccagcagcag cagccccatg
      781 accaccagca tggcgaacgt cttgcccttg cccatggcgc gcgatgcgat caccacgtcg
      841 gcggtggcag ccacctggcg cacgccctgg accacgagct cgcggatgag gagcagggcg
      901 accatccagg tcggcagcag gccgacctcg accatggcca ccaggacccc ggccaccagc
      961 accttgtcgg cggtcaggtc caggaagatg ccgagcgggc tgacctggcc gcgggcgcgg
     1021 gcgatcgccc cgtccaacga gtcggtggcc gccgccaggg cgaacacgat gaatgccgcg
     1081 aggccggcgc cggggaaggc ggtcagcatg aggaccacga ccaccggcgt ggccaggatg
     1141 cgcgccacgc ccagcaggtt cgggagcgtc gtcagccgcg aggcctgacg gggcctggcg
     1201 tcagccgcct cgtccatggg ttaccacctc cttgtccccc agcctgcgct ccgtacccgc
     1261 gcggagccgg gacatattgt cggcgtgcga gatcacgacc agcgccccaa cggccaggga
     1321 gtaggcgacc gcggccagcg gcgccatgcc agccgcggcc agagcggcgg tggcgatcgg
     1381 cgcagacacc cccgcgatga tcgagccgag ggaaacgtac cgggtgcgcc aggtggcgcc
     1441 cgcggccagg ggtcccacga tcgccagcgt cagcggggtc aggaccagca ggccgccggc
     1501 cgaggtggcc actccccgcc cgcccgcgaa accgatgaag accgaccagt tgtgcccgac
     1561 cacggctgcc agtccggcgc cggcggccac ccaatcgtcg gcgtctcccg gtccgccgac
     1621 gaacagcatg cgggcgagcg ccacggccag catccccttg gccacgtcca gggcgaagac
     1681 ggcggcggcc cagcgggtgc ccagcgtgcg caacgcgttg gtggca
//
LOCUS       DATACA010000129         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_258633, whole genome shotgun sequence.
ACCESSION   DATACA010000129 DATACA010000000
VERSION     DATACA010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258633"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..887
                     /locus_tag="VHK28_02650"
     CDS             48..887
                     /locus_tag="VHK28_02650"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX2141164.1"
                     /translation="MPAIEPEQAVQVIVGFIRSQLEQAGFGRLVLGLSGGVDSATVAY
                     LAARAIGHENLLAVRMPYRSSSPDSDEHARLVVDDLGCPTELVDITPMVDPMLESISC
                     SDERALRVRRGNVMARQRMIVLYDRSAAWDALVIGTSNKTEALLGYGTLFGDMAAALQ
                     PIGDLYKSQLRSVAAVLGVPEVILDKPPSADLWPCQTDEGELGATYDDLDRVLYALVD
                     RRWTVDRCVRAGLDRRLVEWVAGTVARMEYKRQLPPVAKVSLRTPGIDHLYPRRRPGS
                     RRA"
     gene            1308..1751
                     /locus_tag="VHK28_02655"
     CDS             1308..1751
                     /locus_tag="VHK28_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2141165.1"
                     /translation="MNKVLLVGRLTRDPELRTLPSGKPVANFTIATNEYRGPAGERTE
                     YHNLVAWDRLAEICGKFLSKGQLVDVEGRLQTRQWDDDAGVRHWKTEIVAASLEMLSG
                     RGKKEYAKEAQIVAEAGPDGTPAQPVAAAAVTNGAGEAEEALVAL"
     gene            complement(1807..2316)
                     /locus_tag="VHK28_02660"
     CDS             complement(1807..2316)
                     /locus_tag="VHK28_02660"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2141166.1"
                     /translation="MNGSLMTDAFGHHVWATLRLIDACAPLNEEQLQSSVPGTYGSIL
                     DTMRHLVGADNRYLFVLTGERVGIIDEDRMSLPELREVMESYGREWSALLDGAIDPDE
                     VLVRRRDDGSETHAPVGIRLAQALHHGTDHRSQICTALTNLGVEPPFIDVWDYGEAQG
                     RTTEIPPSS"
BASE COUNT          353 a          815 c          894 g          371 t
ORIGIN      
        1 cccgacgagg cgggggagtg agcccgccgg aggccgagcg cgcgccactg ccggccatcg
       61 agccggagca ggcggtgcag gtcatcgtcg gcttcatccg ctcgcagctg gagcaggccg
      121 ggttcgggcg cctggtgctc ggcctgtcgg gcggggtcga ctcggccacg gtcgcgtacc
      181 tggcggcgcg ggccatcggc catgagaacc tgctggcggt gcgcatgccc taccgcagct
      241 cgtcacctga ttcggacgag cacgcgcggc tggtggtgga cgacctgggc tgcccgaccg
      301 agctggtgga catcacgccg atggtcgacc ccatgctgga gtcgatctct tgctcggacg
      361 aacgcgcgct gcgcgtgcgc cgcggaaacg tcatggcccg ccagcggatg atcgtgctct
      421 acgaccgatc ggccgcctgg gatgcgctgg tgatcggcac ctcgaacaag accgaggcgc
      481 tgctgggcta cggcacgttg ttcggcgaca tggccgccgc tctgcagccg atcggcgacc
      541 tgtacaagtc gcagctgcgg tcggtggcag ccgtcctggg cgtgccggag gtcatcctgg
      601 acaagccgcc gtccgccgac ctgtggccgt gccagaccga tgagggtgag ctgggcgcca
      661 cctacgacga cctggaccgc gtcctgtacg ccctggtcga ccggcgctgg acggtggacc
      721 gatgcgtccg ggccgggctc gaccgccggc tcgtggagtg ggtggcgggg accgtggcgc
      781 gcatggagta caagcgccag ctgccccctg tcgccaaggt cagcctgcgg acgccgggga
      841 tcgaccacct ctacccgcgg cggcgccccg ggtctcgtcg ggcgtaggtg ccgggccgcc
      901 ggccggcccg tgcgtcacgg ctgatgcagc ttgtcgacgg cgtggcccgt ggcgcgtccg
      961 catcggctac atccttggga attgcggtac cggtagcgcc ttaacgatca cgattccgcc
     1021 tgcccggctc ggtgatgacc gggatcgtgg ctgtagcgcc gcgcgggtgc cgcatttcct
     1081 cacgaaaccg ggtgtcgcgg tcggttggcg cccgggatcg tggacaacca gcttcaccag
     1141 cacggcgcgc caacccatct gtgggggtcc cagcggctgc tggtaagccc gcggcaactg
     1201 gccgataaca ggcgctcctc tagcctgtcg ctccgatacc cggtggccgc ctcgcccggt
     1261 gcgccggcac agctctcagt agcgagggca ggaggagata cgcacccatg aacaaggtgc
     1321 ttctggttgg ccggctgacg cgcgatcccg agctgcggac gttgccgtcc gggaagccgg
     1381 tcgccaattt cacgatcgcg accaacgagt accgggggcc ggccggcgaa cggaccgagt
     1441 accacaacct ggtcgcctgg gaccgcctgg ccgagatctg tggcaagttc ctgtcgaagg
     1501 gacagctggt ggacgtcgag ggccggctgc agacccgcca gtgggacgat gacgctgggg
     1561 tgcggcactg gaagaccgag atcgtggccg cgtcgctcga gatgctgtcg ggccgtggca
     1621 agaaggagta cgccaaggag gcgcagatcg tggccgaggc gggcccggat gggacgccgg
     1681 ctcagccggt cgccgctgcg gcggtgacca acggcgccgg cgaggcagag gaagcgctgg
     1741 tcgccctcta gccgcgccag ggcggggcgg cgagtcgcgc gccgctccgc ttcattccgg
     1801 ctcggctcag gacgacggcg gaatctcggt cgttcgcccc tgcgcctcgc catagtccca
     1861 gacgtcgatg aacggcggct ccacgccgag attggtgagt gccgtgcaga tctggctgcg
     1921 gtggtcggtg ccgtgatgga gcgcctgggc gagacggatg ccgaccggcg cgtgcgtctc
     1981 cgagccgtcg tcgcggcgcc gcaccaggac ctcgtcggga tcgatcgcac cgtccaggag
     2041 cgcagaccac tcgcggccgt agctctccat cacctcccgc agctccggca ggctcatccg
     2101 gtcctcgtcg atgatgccca cccgctcgcc ggtgagcacg aagaggtatc ggttgtcggc
     2161 gccgaccagg tggcgcatgg tgtccaggat cgagccgtac gtgccgggta cgctcgactg
     2221 cagctgctcc tcgttcagcg gtgcgcaggc gtcgatcagg cgcagcgtgg cccagacgtg
     2281 gtggccgaag gcatcggtca tgagggatcc gttcatccat gcattatcgc ggcggccgcc
     2341 gtcaccggca gggctagcgg ctggcggcca ccatgaccag cgtaaggata tatacgtgca
     2401 gcgcggcccc gtacaggatc gagcgcgtgc gcc
//
LOCUS       DATACA010000130         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1303091, whole genome shotgun sequence.
ACCESSION   DATACA010000130 DATACA010000000
VERSION     DATACA010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303091"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VHK28_02665"
     CDS             complement(<1..1520)
                     /locus_tag="VHK28_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX2141167.1"
                     /translation="MTDDTRDIKRPDQPDEAGQAQHDQPENNQAEHGAVSGATDPDRI
                     QVLALVALRDTVIFPEMIVPLQVGRDRSVKALDRAVRASQPVALVTQRSSETEEIGGV
                     DELYAIGTLAKIAQVIRLQDGTIRAIVQGQRRIRLLDLLQNEPHLEARVEVIPDVDGS
                     SLEVEALMASVKAQIEQYVSSGASVPPEVAVAARNISDGGLLADMVAYSPEMTTEQRQ
                     DLLETLDVAERLRKVSQFLAKQIEVLELKGKIQSEVKSEMDKTQREYILREQMKAIQK
                     ELGEDDPAVAEATELRTKVEESAMPDEVKEKALKEVDRLARIPSASPEQGVIRTYVDW
                     LVSLPWGEETDDNLDLSEAEKVLNEDHYGLEKPKERIVEYMAVRKLAEKIRSPILLFV
                     GPPGVGKTSLGKSIARAMGRKFVRMSLGGIHDEAEIRGHRRTYIGALPGRVIQSIKTV
                     GSRNPVFMLDEIDKVGMDFRGDPSSALLEVLDPEQNNTFQDNYLEVPFDLSKVLFIA"
     gene            complement(1539..1883)
                     /locus_tag="VHK28_02670"
     CDS             complement(1539..1883)
                     /locus_tag="VHK28_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2141168.1"
                     /translation="MSSLRDPHRPRYFISIAAELASVHPRTLRIYEEEGLLCPQRRNR
                     LRLYSEADIQRVRIIRFLTRRHGVNLAGVKVILQLEALGKIRVYDLFAEDDLERFIEG
                     DEPAEEPAADLA"
     gene            complement(1954..2445)
                     /locus_tag="VHK28_02675"
     CDS             complement(1954..2445)
                     /locus_tag="VHK28_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2141169.1"
                     /translation="MANITRFDPFGEMVTLRQAMDRLFEDSFVSPLSLRTSNGEAASP
                     ALDVHQNADEIVVTAALPGIKPEDVDITITGQTLSIRGELKADEKVERDQYLYRERRF
                     GTFHRQLQLPVRVQGDAATATFENGVLTLAIPKAEEVKPRQIQVKAAPKQVESSTGGN
                     ETA"
BASE COUNT          355 a          900 c          879 g          497 t
ORIGIN      
        1 gcgatgaaca gcaccttgga caggtcgaac ggcacctcca ggtagttgtc ctggaaggtg
       61 ttgttctgct ccgggtccag cacctccagc agcgccgacg acgggtcgcc gcggaagtcc
      121 atgccgacct tgtcgatctc gtcgagcatg aacaccgggt tgcggctgcc cacggtcttg
      181 attgactgga tcacccgacc cggcagggcg ccgatgtacg tgcggcggtg gccccggatc
      241 tcggcctcgt catggatgcc gcccaggctc atgcgcacga acttgcggcc catggcccgc
      301 gcgatggact tgcccaggct ggtcttgccc acgccgggcg gtcccacgaa gagcaggatg
      361 gggctgcgga tcttctcggc cagctttcgc acggccatgt actcgacgat ccgctccttg
      421 ggcttctcca ggccgtagtg gtcctcgttg agcaccttct cggcctccga caggtcaagg
      481 ttgtcgtccg tctcctcgcc ccacggcagg ctgaccagcc agtcgacgta ggtgcggatg
      541 acgccctgct ccgggctggc gctggggatg cgggccaggc ggtcgacctc cttgagggcc
      601 ttctccttga cctcgtccgg catggccgat tcctcgacct tggtgcgcag ctcggtggcc
      661 tcggccacgg ccgggtcgtc ctcgcccagc tccttctgga tggccttcat ctgctcgcgg
      721 aggatgtact cgcgctgggt cttgtccatc tcggacttga cctcgctctg gatcttcccc
      781 ttgagctcca ggacctcgat ctgcttggcc aggaactggc tcaccttgcg cagccgctcg
      841 gcgacgtcca gcgtctcgag caggtcctgc cgctgctcgg tggtcatctc cggcgagtag
      901 gccaccatgt cggccagcag gccgccgtcg ctgatgttgc gcgcggcaac cgcaacctcc
      961 ggtgggaccg atgcgcccga gctgacgtac tgctcgatct gcgccttgac cgatgccatg
     1021 agcgcctcga cctccagcga tgagccatcc acgtcgggga tgacctccac gcgggcctcg
     1081 aggtgcggct cgttctgcag caggtcgagc agccggatgc ggcgctgccc ctgcacaatg
     1141 gcgcggatgg tgccgtcctg caggcggatg acctgggcga tcttggccag ggtgccgatg
     1201 gcgtacagct cgtccacgcc gccgatctcc tccgtctccg aggagcgctg ggtgaccagg
     1261 gccaccggct gggaggcgcg cacggcgcgg tcgagggcct tgacgctgcg gtcgcggccg
     1321 acctgcagcg gcacgatcat ctccgggaag atgacggtgt cgcgcagcgc caccagggcc
     1381 aggacctgga tccgatccgg atcggtcgcg cccgacacgg cgccgtgctc ggcctggttg
     1441 ttctccggct ggtcgtgctg ggcctggcca gcctcgtcgg gctggtcggg ccgcttgatg
     1501 tctcgggtgt cgtcagtcat atctttcaag aagtcccttc aggcgaggtc ggccgccggc
     1561 tcctcggccg gctcgtcccc ctcgatgaat cgttcgaggt cgtcttctgc aaacaggtcg
     1621 tacacgcgga tcttgccgag cgcctcgagc tgaaggatga ccttgacgcc cgccaggttt
     1681 accccgtggc gacgggtgag gaagcggatg atgcgcaccc gctggatgtc cgcctccgag
     1741 tagagccgca gcctgttccg ccgctgcgga cagaggagcc cttcctcttc gtagatccgc
     1801 agcgtgcgtg gatgcacgct cgccagctcg gcggcgatgc tgatgaagta gcgcggccgg
     1861 tgcgggtcgc ggagcgagct catcgcccgc tgcccgacct cacgccgccg gccgcgctcg
     1921 gcggttcgct gggttcgccg ctgagctgac ggctcacgcg gtctcgttgc caccggtcga
     1981 agactcgacc tgcttcggcg cggccttgac ctggatctgc cgcggcttga cctcctcggc
     2041 cttcgggatg gcgagcgtca gcacgccgtt ctcgaacgtg gccgtggccg cgtcgccctg
     2101 gactcgcacc ggcagctgga gctggcggtg gaacgtcccg aaccggcgct cgcggtacag
     2161 gtactggtcg cgctcgacct tctcgtcggc cttgagctcg ccgcgaatcg acagcgtctg
     2221 gcccgtgatc gtgatgtcca cgtcctccgg cttgatgccg ggcagggcgg ccgtcacgac
     2281 gatctcatcg gcgttctggt gcacgtcgag cgccgggctc gcggcctcgc cgttcgacgt
     2341 tcgcaggctg agcgggctca cgaacgagtc ctcgaagagg cggtccatcg cctgtcgcag
     2401 cgtgaccatc tcgccgaacg gatcgaatcg agtgatgttc gccatgggaa tgctccttcg
     2461 aacctccttc acggtactgg tgaaatcgga ccgtggcgtt accctaaacc ctgacaatag
     2521 ggttgtcaag agtgttgcca tgaccgatgc cggccggggt gccctcgtcc ggctcagcgg
     2581 cggagcctgg cccgcacgga gttcaggcgc ggcatcgcac ggcgtgcctc c
//
LOCUS       DATACA010000131         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2588538, whole genome shotgun sequence.
ACCESSION   DATACA010000131 DATACA010000000
VERSION     DATACA010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588538"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VHK28_02680"
     CDS             <1..615
                     /locus_tag="VHK28_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2141170.1"
                     /translation="ASGTDWLAVAGTVVYYFHFVLPVVTALLLWRRVPLQLHPYLISL
                     ILLSFAGFATYLLLPVAPPWLAAQLGQIGATASEPMVHDLKLHAFTEVTGAVGLDGES
                     LYDVAFIALSANPVAAFPSLHAAFAFLSFLVLRRAFGRAGWLGLMYFGMVAFSIVYSA
                     DHYVIDVLAGVAYASAAYGLMWLLVRPRMEPVEPSPNVPQTAPG"
     gene            complement(622..1428)
                     /locus_tag="VHK28_02685"
     CDS             complement(622..1428)
                     /locus_tag="VHK28_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX2141171.1"
                     /translation="MGQPTISLDDEHARASLGPEEFDRGWLKTHLAEERAKASLLGEI
                     REAIFGAQDGLVSTLAVVSAVAGASAAVFPVLVAGIASGLAGVFSMAAGEYMSSKSQR
                     EIFEAQIVGERDEVHERPGEAEAEMAFMFEEDGLPAEEARSVATILARHPDVLLKTMV
                     EKELGLAGEHAEGSPLQGALIMGGAFGAGALVPIIPYLVLSLETAAVASGVATAAVLF
                     GIGVTKSRWTGRSWLRSGLEILLLGAFAGIAGYFFGSLLPGMLGVPPVGA"
     gene            complement(1449..2057)
                     /locus_tag="VHK28_02690"
     CDS             complement(1449..2057)
                     /locus_tag="VHK28_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX2141172.1"
                     /translation="MAVTRPARHLTLADALVPAVEGRAAGLLRDVALVAGGAALTAIA
                     AQVAFRMPWTEVPYTAQTGAVLLVGTALGWRRGLLSMVLYLLVGVAGAPVFSAGNHGL
                     GQLLGVTGGYLVGFVVAAALVGRLAERGWDRSPLRAAGLMAIGNLAIYAIGVPVLAAA
                     SGLALTDAAWRGAGVFLPWDAFKVLLAAGLLPLAWRLAGRDA"
BASE COUNT          339 a          842 c          761 g          319 t
ORIGIN      
        1 gcatcgggaa ccgattggct ggcggtggca ggcaccgtcg tctactactt ccacttcgtc
       61 ctgccggtcg tcacggcgct gctgctgtgg cggcgcgtcc cgctgcagtt acacccgtac
      121 ctcatctccc tcatcctgct cagcttcgcc ggcttcgcca cctacctgct gctcccggtg
      181 gcgccgccct ggctggctgc tcaactgggg cagatcggtg cgacggcttc ggaaccgatg
      241 gtgcacgacc tcaagctgca cgccttcacc gaggtcaccg gtgctgtcgg cctggatggc
      301 gagagcctgt acgacgtggc tttcatcgcc ctgtcggcga acccggtggc cgccttcccc
      361 tcgctgcacg cggcgtttgc cttcctctcc ttcctggtgc tgcgacgggc gttcggcagg
      421 gcgggctggc tgggcctgat gtacttcggg atggtggcct tcagcatcgt gtactcggcc
      481 gatcactacg tcatcgacgt gcttgccggc gtggcctacg cgtcggccgc gtacgggctg
      541 atgtggttgc tcgtccgtcc gcggatggag cccgtcgagc cgtcgcccaa cgttccgcag
      601 acagcccccg gctgaggtgc ctcaggcgcc caccggcggg acgcccagca tgccgggcag
      661 gaggctgccg aagaagtagc cggcgattcc ggcgaaggcg cccagcagca ggatctcgag
      721 ccccgagcgc agccagctgc gccccgtcca gcgcgacttg gtgacgccga tgccgaacag
      781 cacggccgcg gtcgccaccc cggacgccac ggcggctgtc tccagcgaca ggaccaggta
      841 ggggatgatg ggcaccaggg caccggcgcc gaaggcaccg cccatgatca gcgccccctg
      901 caacggggag ccctccgcgt gctcgccggc caggcccagc tccttctcga ccatcgtctt
      961 gagcagcacg tccgggtgac gggccaggat ggtggccacc gatctggcct cctcggccgg
     1021 gagcccatcc tcctcgaaca tgaaggccat ctcggcctcc gcctcgccgg gacgctcgtg
     1081 gacctcgtcc cgctccccga cgatctgggc ctcgaaaatc tcgcgctggc tcttgctgct
     1141 catgtactcg ccggcggcca tgctgaagac gcccgccagg ccggaggcaa tgccggcgac
     1201 caggaccggg aagaccgccg cggacgcgcc ggccaccgcg ctgaccacgg ccagcgtgct
     1261 caccaggccg tcctgggcgc cgaagatcgc ctcccggatc tccccgagga ggctggcctt
     1321 ggcgcgctcc tcggccaggt gggtcttcag ccagccccgg tcgaactcct ccgggcccag
     1381 ggacgcgcgg gcatgctcgt cgtccaggct gatggtcggc tgtcccatgg aagggatgct
     1441 agccgaggtc aggcatcgcg gccggccagg cgccacgcca ggggcagcaa ccccgcagcc
     1501 agcagtacct tgaacgcgtc ccagggaagg aacaccccgg ccccgcgcca ggcggcatcg
     1561 gtcagcgcca ggccggaggc cgcggccagg acgggcacgc cgatggcgta gatggccaaa
     1621 ttgccgatgg ccatgagccc cgcggcgcgc aggggcgacc gatcccaccc ccgctccgcc
     1681 agccggccga ccagcgccgc ggcaaccacg aatcccacca ggtagccgcc ggtgacgccc
     1741 agcagctgcc ccagtccgtg attgccggcc gagaagacgg gtgccccggc cacgccaacc
     1801 agcaggtaca gcaccatgct gagcaggccg cgccgccagc ccagggcggt gccgaccagc
     1861 aggacggcgc cggtctgcgc cgtgtacggc acctccgtcc acggcatccg gaaggcgacc
     1921 tgcgcggcga tggcggtcag cgcggcgcca ccggccacca gcgcgacgtc gcgcagcagg
     1981 ccggccgctc gaccctcgac ggcggggacc agggcatcgg ccagggtcag atgccgagct
     2041 ggacgcgtga cggccatggg ttcgatacct cctcgttggc caacgtagat ggacggatcg
     2101 tagcgggccg gcgccactcc cagcgcaccc ggtgcggtac actgcgccag gtcctttaag
     2161 tccggcacag agaggccgga aaggagcgag acgtgcacac cctgccgcag actgccatac
     2221 cgacgccaga cgccgatggg cggccggcgt cgttttcatc t
//
LOCUS       DATACA010000132         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_500027, whole genome shotgun sequence.
ACCESSION   DATACA010000132 DATACA010000000
VERSION     DATACA010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500027"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VHK28_02695"
     CDS             <1..977
                     /locus_tag="VHK28_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141173.1"
                     /translation="ALQRRPPRRGLDPAGRAAAELRPPRSMLGLLLPVLLPVAERIAQ
                     WLPRPAAYAVADLAGRAWHRWSSARRRVVAANLARVCAATGRPTSGRQFHDIVRRAFV
                     EHARYWLEVFRAPGWSAKRLDRMLSADDWAHWQPIFAAGAVVGVPHLGNFEPFAHFVA
                     VHGISGVAPVEETRPFALYRFLLKRRLVGGQGVQLIPLSRAMRPMLVALRQNRLVALA
                     ADRDLTGTGVEVTFFGHPARIPAGPATLALRTGKPLLVARVLRTAPEQFHGRAWALDV
                     ERSGDTARDVATLTEAMARRFEQAIAEAPEQWWGAFQPFWPDQLEPRS"
     gene            974..1720
                     /locus_tag="VHK28_02700"
     CDS             974..1720
                     /locus_tag="VHK28_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEX2141174.1"
                     /translation="MTSRGADQRGKADMHLHTLYSDGTAEVRALLDHVEQHTDLDLIA
                     VTDHERIDGAVRARELAADGGYSFEVVVGEEITTRRGHLLALFIEERIPPLRPITETL
                     ERIHDQGGLAIAAHPMAPLTPSLGRRTLLELHADRDPRHRLDAIEILNPSAAGRARRL
                     ARHHLNEDVLHLTEVGNSDAHVLEHVGSGWTWYPGSTAEHYRRAVLDRTTEADGEHWS
                     HWHNVTVYGRQLVAKARHLRHTLRPTGEWR"
     gene            1717..>2546
                     /locus_tag="VHK28_02705"
     CDS             1717..>2546
                     /locus_tag="VHK28_02705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2141175.1"
                     /translation="MTKAEPAAGRRAAAPSVQPARERPPLKVGIVSPYGYPHPGGVNE
                     HVRHTYEAMRRMGHDVWIITSKYGKERESEGHIIRLGTGYAFPANGSVGRVTVSWRFK
                     ERARALLAEHRFDILHFHEPLVPFLSPTVLDQSDTVNIGTFHAFGGFSPSYWVGKRFA
                     GRLAKRLDGRIAVSGAARHFIDRYFPGEYRIIPNGVDLDRFADAEPFDELRDGTLNIL
                     FLGRFEERKGLIHLLKAYHRLRKRHVDARLLVVGSGPKAREYKRYVGLRQIRDVEFLG
                     R"
BASE COUNT          362 a          941 c          891 g          352 t
ORIGIN      
        1 gcgcgctcca gcgccgcccg ccgcggcgag gactggaccc ggccggccgt gccgcggccg
       61 agctgaggcc tccccggtcg atgctcgggc tgctgctgcc ggtcctcctc ccggtcgccg
      121 agcgcatcgc ccagtggctg ccgcggccag cggcctatgc cgtggcggac ctggccggtc
      181 gtgcctggca ccgctggtcg tcggcgcgac gcagggtggt ggccgccaac ctggcccgcg
      241 tctgcgccgc caccggccgg cccacctctg ggaggcagtt ccacgacatt gtgcgacgcg
      301 ccttcgtgga gcacgcccgc tactggctgg aggtcttccg ggcccccggc tggagcgcaa
      361 aacggctgga ccgcatgctc agcgccgacg actgggcaca ctggcaaccg atcttcgccg
      421 ccggcgcggt ggtcggcgtg ccgcaccttg gcaacttcga gccgttcgcc cacttcgtgg
      481 cggtgcacgg catcagcggc gtggcgccag tcgaggagac ccggcccttc gcgctgtacc
      541 gcttcctgct caagcgccgg ctggtgggcg gccagggcgt gcagctgatc ccgctgtcgc
      601 gcgcgatgcg ccccatgctg gtcgccctgc gccagaaccg gctggtcgcc ctggccgcgg
      661 accgcgacct taccgggacg ggcgtggagg tcaccttctt cgggcacccc gcgcgcatcc
      721 cggccgggcc agcgaccctg gcgctgcgga ccggcaagcc cctgctcgtg gcgcgcgtgc
      781 tgcgcaccgc accggagcag ttccacggtc gcgcctgggc gctcgacgtg gagcgcagcg
      841 gcgacaccgc gcgggacgtc gccacgctga ccgaggccat ggcccgccgc ttcgagcagg
      901 cgattgccga ggcaccggag cagtggtggg gggccttcca gccgttttgg cccgatcagc
      961 tggagccgcg gtcatgacct cgcgcggggc cgaccagcgc ggcaaggcgg acatgcacct
     1021 gcacaccctc tactcggacg ggaccgcgga ggtccgggcg ctgctcgacc acgtggagca
     1081 gcacaccgac ctggatctga tcgcggtcac cgaccacgag cggatcgacg gcgcggtgcg
     1141 cgcccgcgag ctcgccgcgg acggcggcta ctccttcgag gtggtggttg gcgaggagat
     1201 caccacccgg cgtggccacc tgctggcgct attcatcgag gagcggatcc caccgctgcg
     1261 ccccatcacc gagacgctgg agcgcatcca cgaccagggc gggctggcca tcgccgccca
     1321 ccccatggcg ccgctgaccc ccagcctggg acgccgcacg ctgctcgagc tgcacgccga
     1381 ccgcgacccg cgccaccggc tggacgccat cgagatcctg aacccctccg ccgccgggcg
     1441 tgcgcggcgc ctggcgcgtc atcacctcaa cgaggacgtg ctgcacctca ccgaggtcgg
     1501 caacagcgat gcccacgtgc tggagcacgt cggcagcggc tggacctggt acccgggatc
     1561 gacggccgag cactaccggc gtgccgtgct ggaccgcaca accgaggcgg acggcgagca
     1621 ctggagccac tggcacaacg tgacggtgta cggccgccag ctcgtggcca aggcgcggca
     1681 cctgcggcat actcttcggc ccacaggaga gtggcgatga ccaaggccga gccggcagcg
     1741 gggcggcgtg cggctgctcc atcggtccag cctgcgcgtg agcgcccacc actcaaggtc
     1801 gggatcgtca gcccgtacgg ctacccgcac cccggcgggg tgaatgagca cgtgcggcac
     1861 acctacgagg ccatgcgccg catgggccac gacgtgtgga tcatcaccag caagtacggc
     1921 aaggaacggg agagcgaggg ccacatcatc cgcctgggca cgggctacgc cttcccggcc
     1981 aacgggagcg tgggccgcgt gacggtcagc tggcgcttca aggagcgggc gcgcgctctg
     2041 ctggccgaac atcgcttcga catcctgcac ttccacgagc cattggtgcc gttcctgtcg
     2101 ccgaccgtgc tggaccagtc ggacacggtc aacatcggca cctttcacgc ctttggcggc
     2161 ttctcgccct cgtactgggt cggcaagcgc ttcgccggtc gcctggcgaa gcggctcgac
     2221 ggccggatcg cggtcagcgg cgccgcgcgg cacttcatcg accgctactt tccgggcgag
     2281 taccgcatca tccccaacgg cgtcgacctg gaccgattcg ccgatgccga gcccttcgac
     2341 gagctgcgcg acgggacgct caacatcctg ttcctgggtc gcttcgagga gcgcaagggg
     2401 ctgatacacc tgctgaaggc ctaccaccgg ctgcgcaagc gccacgtcga tgcgcggctg
     2461 ctggtggtcg gctcaggccc caaggcgcgg gagtacaagc gctacgtggg gctgcgccag
     2521 atccgcgacg tggagttcct gggccg
//
LOCUS       DATACA010000133         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_500058, whole genome shotgun sequence.
ACCESSION   DATACA010000133 DATACA010000000
VERSION     DATACA010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500058"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VHK28_02710"
     CDS             <1..1533
                     /locus_tag="VHK28_02710"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2141176.1"
                     /translation="LPVEAGRVDVRRLLPGTVPGQPGVLPRPEERAETLCLHFLRAER
                     YLEAWKYARLAGDRAREKAANVDAAGFYRQALDAAQLSGQARPDEVAALAEKLGDVCE
                     IAGLYQDGLRAYRTARRVRAGMGTVPPDLFRKEGLVQERLGRYPKAEALYRQGLRVAA
                     SMTDEEEAARSRARVQVAMAGALQYEGRLRAGLWWARKGARLAAEHDVMDVLAHAYYL
                     IDNAYTDLGDQAAAKEYRGLALPIYQQLGDLIGQVKVLNNLGANAYLEGQWNEAIAFY
                     ERGLDAAERAGDTVHVAMLSTNIAEVLLQQGHHEEAERLLKEALQTQRGTGHRIGEAW
                     SQFNLGVSAARAGRTEEAYNLLTQAREKFSEMGAESYVLEVSARLAEVHLVAGDHRSA
                     RATLSRAMRDVEARGGLVLEALMRRLQAYALLQAGDPDQALAVLERSCYVAEMANATY
                     ELALTLDAMWRFGEVLGRPRPEERRRASSLLEDLGVVRTPVIPLPSDVEPAASGSHGL
                     RN"
     gene            1656..2159
                     /locus_tag="VHK28_02715"
     CDS             1656..2159
                     /locus_tag="VHK28_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141177.1"
                     /translation="MSAIRTFSIVTWVLLPTVMFGGYSLLRLMNAEELSDFQLTFFRA
                     GHAHAGVLLVMALVYFSFLARTSLSDSLKLWACVILMLGVLMQSGGFFLHMAVGEQGE
                     SSPGILVTTIGALLLTVAIGLLVYGLIITKSSPFGLPSEAAYGTSAVAAGDAPRGGSG
                     PAPTAPV"
     gene            complement(2143..3312)
                     /locus_tag="VHK28_02720"
     CDS             complement(2143..3312)
                     /locus_tag="VHK28_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2141178.1"
                     /translation="MRRPRVLVVGGGIGGMTAAIALRRRGLDAVVFERAASIERVQLG
                     AGLHLWSNALRALQVLALDRAVVDIGTVMTRQRYLDSRGRSLGDLDVGRVSSELGAPT
                     VAVTRPDFHRLLVDALAEVGPDALRFGAECTGFYHEPDSVTAKFADGTEERGDVLVGA
                     DGIASAVRAQLHGAEPPSYSGYTAWRALCDFSHPRLPVGEMWIYWGPGARILHYHVSD
                     QRVYWLAMAKAPARTPDPPEGRKAAALARYRGWASPVEELIESTDEAAIIHQDVVDRD
                     PIPRWGASRVTLLGDAAHPMTPNLAQGAGQAIEDGVSLATVLASSADPVEGLRAYERR
                     RAERANGMIKVSRMVGRMSLLESPVARAVRDNVVLRSIYATYDGRRVRRDLTPVL"
     gene            complement(3355..3810)
                     /locus_tag="VHK28_02725"
     CDS             complement(3355..3810)
                     /locus_tag="VHK28_02725"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HEX2141179.1"
                     /translation="MNLADIAVVVATVLVGLLAGNELGTLSVVHPALEKAGYPAGRPG
                     AQAIVARYGKVMPAAMPLTIAVTFLTAALLDGTQTVLMLIAGALLVVMLLVTLVGLAP
                     LNSRQIAATESTPAEEWRGWRKRWLKLHTVRVVCDITALVLVATAALLR"
     gene            complement(3807..4250)
                     /locus_tag="VHK28_02730"
     CDS             complement(3807..4250)
                     /locus_tag="VHK28_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2141180.1"
                     /translation="MPSVASRAQNFFPNLILRSPLHPVLSGKYTIIEFTGRKSGRKYS
                     TPVAYVKEGERILMTTDSPWWRNMSGGAPVKVWVGGRRREGTATAVTDPERSAAILRM
                     LVDAIPSYAGPAELAREGGRVSDAEIARAVAEGRVAIEVDVEGRA"
     gene            4435..>5414
                     /locus_tag="VHK28_02735"
     CDS             4435..>5414
                     /locus_tag="VHK28_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX2141181.1"
                     /translation="MADGRGSDRLGGNAEQRRLAEPFEGRVQPWRRWGPYISERAWGT
                     VREDYSPDGEAWDHFPHELARSKAYRWGEDGIAGLCDRYQKLCTSLALWNGRDPILKE
                     RMFGLGHHEGNHGEDAKELWHYLDATPTHSYGRMLYRYPQAEFPYLELVEENRRRGGH
                     GPEYELVDTGIFDDGRYWDVEVEHAKAAPEDLCIRITAVNRGAEPASLHLLQQLWFRN
                     TWSWGHPRGLPPVITAADAPAGCIAVCADDSAASPLPGLRFDYRVGRRHLYGPSPGAL
                     LFTDNETNIERVYGCPSHSRFTKDAFHRFIFDCEDCCNPEETGTKAALHYV"
BASE COUNT          836 a         1900 c         1905 g          773 t
ORIGIN      
        1 ctgccggtag aagccggccg cgtcgacgtt cgccgccttc tccctggcac ggtccccggc
       61 cagccgggcg tacttccacg ccccgaggag cgcgccgaga cgctgtgcct ccacttcctc
      121 cgggccgagc gctacctgga ggcgtggaag tacgcccggc tggccgggga ccgtgccagg
      181 gagaaggcgg cgaacgtcga cgcggccggc ttctaccggc aggcgctcga tgcggcgcag
      241 ctgtcgggtc aggccaggcc ggatgaggtc gccgcactgg cggagaagct cggtgacgtc
      301 tgcgagatcg cgggcctgta ccaggacggg ttgcgtgcct accggacggc gcggcgggtt
      361 cgcgccggca tgggaaccgt ccccccggac ctgttccgca aggaggggct cgtccaggag
      421 cggctggggc gctacccgaa ggcggaggcc ctgtaccggc agggcctgcg cgtggcggcc
      481 tcgatgacgg acgaggagga ggcggcccga agccgcgcca gggtccaggt ggcgatggcg
      541 ggagccctcc agtacgaggg tcgcctgcgc gccggtttgt ggtgggcgcg caagggcgcg
      601 cgcctcgccg ccgagcacga cgtgatggat gtcctggccc acgcctacta cctgatcgac
      661 aacgcctaca ccgacctcgg ggaccaagcg gcagccaagg agtaccgcgg gcttgcgctg
      721 cccatctacc agcagctcgg tgaccttatc ggccaggtga aggtgctcaa caacctgggc
      781 gcgaacgcat acctggaggg ccagtggaac gaggcgatcg ccttctacga gcgcggcctc
      841 gacgccgccg agcgcgccgg cgacaccgtt cacgtcgcga tgctgtcgac caacatcgcc
      901 gaggtgctgc tccagcaggg ccaccacgag gaggcggagc ggctgctcaa ggaggcgctc
      961 cagacgcagc gcggcacggg ccaccgcatc ggcgaggcat ggtctcagtt caacctggga
     1021 gtcagcgccg cccgcgcggg tcgcacggag gaggcgtaca acctcctcac acaggcgcgc
     1081 gagaagttca gcgagatggg cgcggaatcc tacgtgctgg aggtcagcgc ccggctcgcc
     1141 gaggtgcacc tggtggccgg cgaccaccgc agcgccaggg cgaccctcag cagggccatg
     1201 cgcgacgtgg aggcccgcgg cgggctggtg ctcgaggcgc tgatgcggcg gcttcaggcc
     1261 tacgccctgc tccaggccgg ggaccccgac caggcgctcg ccgtgctgga gcgcagctgt
     1321 tacgtggctg agatggccaa cgccacctac gagcttgcgc tcaccctcga tgcgatgtgg
     1381 cgctttggcg aggtgctcgg caggccgcgc ccggaggagc gtcggcgggc aagctcgctg
     1441 ctcgaggatc tcggcgtggt gagaacgccg gtgatccccc tcccgtccga cgtcgaaccg
     1501 gcggcttcgg ggtcgcacgg actgcgcaac tgaccctcct cgagccggcg tcggtgcgct
     1561 agtgtgccgc ccagcggtgc ctggtcggcc cgcagcgctg gcggactgcc ccgaggagtc
     1621 gctcagcgtc acgcagtcgg agcaagggag ccaggatgag cgcgattaga acgttctcga
     1681 tcgtcacctg ggtgcttctg cccacggtca tgttcggcgg ctactcgctg ctgcgcctga
     1741 tgaacgccga ggagctcagc gacttccagc tgaccttctt ccgggcaggg catgcgcacg
     1801 ccggcgttct gctcgtgatg gcactggtgt acttcagctt cctcgcgcga acgtcgctgt
     1861 ccgacagcct caagctgtgg gcctgcgtga tcctgatgct cggcgtgctc atgcagtccg
     1921 gcggcttctt cctgcacatg gccgtcgggg agcaggggga gagctcgccc ggcatcctgg
     1981 tgacgaccat cggggcgctg ctgctgaccg tggcgatcgg cctgctggtc tacggcctga
     2041 tcatcaccaa gtccagcccg ttcgggttgc cgtccgaggc cgcctatggg acctctgcag
     2101 tagcagcggg ggacgctcct agaggcggca gcggccccgc gcctacagca ccggtgtgag
     2161 gtcgcgcctg accctgcgcc cgtcgtaggt ggcgtagatc gagcgcagca cgacgttgtc
     2221 gcgcacggcg cgcgcaacag gcgactccag caggctcatg cgcccgacca tgcgtgacac
     2281 cttgatcatg ccgttggccc gctcggcgcg gcgccgctcg taggcgcgca ggccctcgac
     2341 aggatcggct gacgacgcca ggacggtcgc gaggctgacg ccgtcctcga tcgcctgccc
     2401 cgcgccctgc gcgaggttgg gcgtcatggg atgcgcagcg tccccgagca gggtcacccg
     2461 gctggccccc cagcgcggga tgggatcgcg gtcgacgacg tcctggtgga tgatcgctgc
     2521 ctcgtcggtg ctctcgatca gctcctcgac gggactcgcc cagccgcggt agcgggcgag
     2581 cgcggcggcc ttgcggccct ccggcgggtc gggcgtccgc gcgggggcct tggccatggc
     2641 cagccagtaa acgcgctggt cactcacgtg gtagtgcagg atgcgggcgc cggggcccca
     2701 gtagatccac atctccccga ccggcagtcg agggtgcgag aagtcgcaca gggcacgcca
     2761 ggcggtgtag cccgagtagc tcggcggctc ggcgccgtgc agctgggcgc gaaccgccga
     2821 ggcgatgccg tcagccccca ccaggacgtc gccgcgctcc tcggtaccat ccgcgaactt
     2881 ggccgtgacg ctgtccggct cgtggtagaa gccggtgcac tcggcaccga agcgcagggc
     2941 gtcgggtccg acctccgcca gcgcgtcgac gagcaagcgg tggaagtccg gtcgggtgac
     3001 cgcgaccgtc ggcgcgccca gctcgctgct caccctcccg acgtcgaggt cgcccaggct
     3061 gcgcccgcgc gagtcgaggt agcgctggcg cgtcatgaca gtcccgatgt cgaccacggc
     3121 gcggtcgagc gccaggacct ggagcgcccg cagggcgttg ctccacaggt gcagccccgc
     3181 tccgagctgg acgcgctcga tcgaggcagc ccgctcgaac accaccgcat caagcccgcg
     3241 acgacgcagc gcgatggctg ccgtcatgcc gccgatgcca ccaccgacca ccagaaccct
     3301 aggacgtcgc atgacttgcc ccacctccac tagttggcac ccgcccgctt gaactcagcg
     3361 cagaagggca gcggtcgcga ccagcacgag ggcagtgatg tcgcacacaa ctcggacggt
     3421 gtgcagtttg agccatcgct tgcgccaccc gcgccactcc tcggcagggg tcgactcggt
     3481 cgcagcgatc tggcgagagt tcagcggcgc cagcccgacc agcgtcacga gcagcatcac
     3541 gaccagcaag gctcccgcga taagcatgag caccgtctgg gtgccgtcga gcagcgcagc
     3601 agtgagaaag gtcacggcga tggtgagggg catcgcggcc ggcatgacct tgccgtagcg
     3661 cgccacgatc gcctgcgctc ccggccgccc cgccgggtag ccggccttct ccagggccgg
     3721 gtgcacgact gacaaggtcc cgagctcgtt gcctgccagc agtccgacca gcaccgtggc
     3781 gaccaccacc gcgatgtcgg cgaggttcac gcccggccct ccacgtcgac ctcgatcgcc
     3841 acgcgcccct ccgcgaccgc gcgcgcgatc tccgcgtcgc tcaccctgcc gccctcgcga
     3901 gcgagctctg ccggcccggc gtaggacggg atcgcgtcga cgagcatccg caggattgcg
     3961 gccgagcgct cgggatctgt gaccgcggtg gcggtgccct ccctgcggcg gcccccgacc
     4021 cagaccttga cgggcgctcc cccggacatg ttccgccacc acggcgaatc cgtggtcatc
     4081 aggatgcgtt cgccctcctt gacgtacgcg accggcgtcg agtacttgcg gccgctcttg
     4141 cggccggtga actcgatgat cgtgtacttg ccgctcagga ccgggtgcag gggcgagcgg
     4201 aggatgaggt tggggaagaa gttctgggca cgggacgcaa ctgagggcat ggggcactcc
     4261 cgataaaaag ttcggtgtgc acaaacctag acgtgtccgg cgccgataca agggggctga
     4321 tggccgccgg aggtacccgc cctgacagac gacggtactg cgtacactcc gcacccggag
     4381 tcgagccgcc tggcgctgcg ctgcttgagc ggtggtcacc tggcggaggg gcgcatggcg
     4441 gacgggcgcg gatcggatcg cctcggtggc aatgccgagc agcgcagact ggccgagccc
     4501 ttcgaagggc gggtgcagcc gtggcgccgc tgggggccct acatcagcga gcgcgcctgg
     4561 ggcaccgtgc gcgaggacta cagcccggat ggcgaggcct gggaccactt cccacacgag
     4621 ctggcacgca gcaaggcata ccgatggggc gaggacggca tcgccggcct gtgcgaccgc
     4681 taccagaagc tgtgcaccag cctcgcgctg tggaacggtc gcgacccgat cctgaaggag
     4741 cggatgttcg gcctgggcca ccacgagggc aaccacggcg aggacgcgaa ggagctgtgg
     4801 cactacctcg acgccacacc gacccacagc tacggacgca tgctctaccg ctatccgcag
     4861 gccgagttcc cgtacctcga gctggtcgag gagaaccggc gccgaggtgg gcacggaccg
     4921 gagtacgagc tcgtcgacac cggcatcttc gacgacggcc gctactggga cgtggaggtc
     4981 gagcacgcga aggccgcacc agaggatctc tgcatacgaa tcaccgcggt caatcgcggc
     5041 gccgagcccg ccagcctgca cctgctccag cagctgtggt ttcgcaacac ctggtcgtgg
     5101 ggccatccgc gcgggctgcc gccggtcatc acggcggctg acgcgcctgc cggctgcatc
     5161 gctgtctgcg ccgacgacag cgccgcctcg ccgctgcccg gccttcgctt cgactaccgg
     5221 gtcgggcgcc gccacctcta cgggccgtcg ccgggcgcgc tgctgttcac cgacaacgag
     5281 accaacattg agcgcgtcta cggctgtccc agtcacagcc gcttcaccaa ggacgccttc
     5341 caccgcttca tctttgactg cgaggactgc tgcaaccccg aggagaccgg caccaaggcg
     5401 gcgttgcact acgt
//
LOCUS       DATACA010000134         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1463621, whole genome shotgun sequence.
ACCESSION   DATACA010000134 DATACA010000000
VERSION     DATACA010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463621"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VHK28_02740"
     CDS             complement(<1..666)
                     /locus_tag="VHK28_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2141182.1"
                     /translation="MSPELDGRLIVGGEWTDGDGTFVVMDPYRSTPVAEVHSASAATV
                     ERAVAKAREGAREMGAMPAHARADLLRRAAMLVSERAEEIATTVSRQTGKALRDTRRE
                     ASRSTHTLRAAATAAETLAGSLEPADAIPGGEGLLAMVMREPLGVIGAIAPFNAPFNL
                     SMHKVAAAIAAGNSVVLKPSSHAPLSGFQLAEILLEAGLPPAAISVLPGSGVGPALVA
                     HAGV"
     gene            complement(656..1084)
                     /locus_tag="VHK28_02745"
     CDS             complement(656..1084)
                     /locus_tag="VHK28_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2141183.1"
                     /translation="MTTDERLAAAWDYAHGYYADPQVEEEFKLLSRFQPEVFTSYMSM
                     RRGLFQAPPDGALDRKTKELVILGIECMARKTNPPPTGHTRKAIEAGATPEEISEIVG
                     LCIMLGGMITFRESGRFVLREAVEHAERQHGAGAAASDES"
     gene            1215..1919
                     /locus_tag="VHK28_02750"
     CDS             1215..1919
                     /locus_tag="VHK28_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2141184.1"
                     /translation="MDADVAVKRLYERFAGNGSTADAIHDAVAEGILSRMLPPGSRLG
                     EERLAALFSVSRTPVREALMRLESENLAARNRRSGLVVADVSAEQILEIYVIREALDG
                     VAARQAAHFHSGADIAALERINDQIERAAAEGAFPRMASLNVEFHTALARASRNQILQ
                     RFVDQVHQAVRRFTRTTFSEPGRAQEAVSEHRQIIDAVKAQDEELAERVARAHMHNAL
                     NVRMAMELDRGIDSDS"
     gene            1959..2435
                     /locus_tag="VHK28_02755"
     CDS             1959..2435
                     /locus_tag="VHK28_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX2141185.1"
                     /translation="MKVTTTLRVNGQLVQSPADPETPLLYVLRNDLGLKGTRFGCGVG
                     LCGACTVQVDGVATASCNLPISALGDRWVTTIESLGDEARLHPLQQAFLDEQAGQCGY
                     CLTGVLMTAAALLDSNPDPSRAEIRSALDGVLCRCGVQDRMVRAVERAAAQLRVQP"
     gene            2432..4546
                     /locus_tag="VHK28_02760"
     CDS             2432..4546
                     /locus_tag="VHK28_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX2141186.1"
                     /translation="MTTDGLPMALRSYPLIGQWLSISPDGQIVVRCGKVEFGQGIRTA
                     QAQIVAHELAVPVDAVTVEEVSTIGSPDEGVTSGSRSIEEADEGLRRAAAELRVALAR
                     RAATRCGVAASDLSVDSGCVVLPDGQRIPYADLVDSDLAAEAISGNASPRPPAAGSPL
                     GASVPRVDLPAKVLGKPAFVGDLELPGMLHGRVCRPAGPGARLVEVDEQAVLEMPGVR
                     AVVRDGNFLAVVAEREEQAIRALARLRRVSTWTEPSTLPSSTRFLLHEQTLDVTVAEA
                     GEPPGDALQVRPLTGEYSRPYLAHASLGPSCAVATFHGDSYDIWTHSQGVYHLRGELS
                     KVLELDAEEIRVHHLEGAGCYGANGADDAALDAALLARAVPGSPVRVQWMRADEFGWE
                     PFGTPMVVRLQALLDESGSIVDWQHDVWGHGHRDRAGSDSPRNVTNLLAARHLANAFE
                     GSVPAPPPTPSAGSGRNAAPVYAFPSQRVINHYVPRVPIRVSALRSLGAHANVFAIES
                     FMDEIAAELGVDPLEHRLRYLHDQRGRRVLKSATERAGWSGRRITEGRGLGIGFAQYK
                     NDGCYVAVVAEVDVDDELVLRRVWAAVDAGLVINPDGLRNQVEGGIAQAASWTLKEQV
                     TFDRSHITSRDWETYPILRFSEAPEIEVDVIVQPDEPPHGAGEAVAGPTAGAIANAVF
                     AATGARIRDMPITRERLIRALA"
     gene            4543..>4723
                     /locus_tag="VHK28_02765"
     CDS             4543..>4723
                     /locus_tag="VHK28_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2141187.1"
                     /translation="MTGVTFGFKTEPPRAEWEDLRRIWQEADEIPLIADLWAFDHFVP
                     LRADAGDPCLEGWTVL"
BASE COUNT          712 a         1601 c         1642 g          768 t
ORIGIN      
        1 cacccctgcg tgcgcaacaa gtgccggacc gacgccggat ccgggcagga cgctgatggc
       61 ggccggcggg agacctgcct ccaggaggat ctcggccagc tggaagccgc tcagcggcgc
      121 gtgggacgac ggcttgagga cgacgctgtt gccggctgcg atggccgccg cgaccttgtg
      181 catcgacaaa ttgaagggcg cgttgaacgg cgcgatggca ccgatgactc cgagtggctc
      241 ccgcatcacc atcgccagca gcccttcgcc gcccgggatc gcgtctgcgg gctccaggct
      301 gcctgccagc gtctcggccg cggtggccgc cgcacgcagc gtgtgggtgc tgcggcttgc
      361 ctcgcgtcgg gtgtcgcgca gcgccttgcc ggtctggcgg ctgacagtgg tggcgatctc
      421 ctctgctcgc tccgagacca gcatcgcagc ccgacgcagg aggtcagcgc gggcgtgggc
      481 cggcatggcc cccatctcgc gcgccccctc acgggccttc gccaccgccc gctccacggt
      541 agccgcactc gcggaatgca cctcggccac gggggtggac cgataggggt ccatgacaac
      601 gaaggtgccg tcgccatcgg tccattcgcc gcccacgatc aggcggccat caagctcagg
      661 actcatctga cgctgcagcc cccgctccat gctggcgttc ggcatgctcg accgcctcgc
      721 gcaggacgaa ccgaccgctc tcgcggaagg tgatcattcc gccgagcatg atgcacaacc
      781 ccacgatctc cgagatctcc tctggcgtgg cgccagcctc gatcgccttc cgggtatgcc
      841 cggtgggcgg ggggttcgtc tttcgcgcca tgcactcgat gccgaggatc accagctcct
      901 tggtcttgcg gtcgagcgcg ccatccggcg gcgcctggaa caggccgcgg cgcatggaca
      961 tgtagctggt gaagacttct ggctggaagc gcgacaggag cttgaactcc tcttccacct
     1021 gcgggtcggc gtagtagcca tgcgcgtagt cccatgcggc ggcgagccgc tcgtcggtcg
     1081 tcatcgatcc cacctccaag tcgctcagta tacggttgtg tacgcctgtt catgaggggc
     1141 gtcagcgacc gccgggtttc ggtatgcttg acctgcccga cgttccggta attgtcgtgg
     1201 gagggtgctc agccatggac gcggatgtcg ccgtaaagcg gctctacgag cggtttgcgg
     1261 gcaatgggtc gactgccgac gctatccacg acgccgttgc cgagggaatc ctgtcacgga
     1321 tgctccctcc cggttcccgg cttggcgagg agcggctggc tgccctcttt tcggtctctc
     1381 gcacgccggt gcgtgaggca ctgatgcgcc tggagagcga gaacctcgcc gcccgcaacc
     1441 gccgcagcgg actggtggtg gccgacgtgt cggcggagca gatcctggag atctacgtca
     1501 tccgggaggc gctcgacggg gtggctgcac gacaggcagc tcacttccac tccggcgccg
     1561 acatcgccgc cctggagcgc atcaatgatc agatcgagcg cgcggctgcg gagggcgctt
     1621 tcccgaggat ggcgtccctc aatgtggagt tccataccgc cctcgcccgt gccagccgca
     1681 accagatcct gcagcgcttc gtggaccagg tgcaccaggc cgtcaggcga ttcacgcgca
     1741 ccacgttctc cgagcccggc cgcgcacagg aggcggtcag tgagcaccgg cagatcatcg
     1801 acgcggtcaa ggcccaggac gaagagctgg ccgaaagggt ggcccgcgcc catatgcaca
     1861 acgcgctgaa cgttcgcatg gcgatggagc tggaccgcgg catcgatagc gactcctgac
     1921 ggcttgccgg gggcgactct cgtgtgcaac cgtatacgat gaaggtgacg actacgcttc
     1981 gcgtcaacgg ccaactggtc cagtcgcccg ccgatccgga aactcccctg ctctacgtcc
     2041 tccgtaacga tctcgggctg aaggggaccc gcttcggctg tggcgttggg ctgtgcggcg
     2101 cctgcacggt ccaggtcgac ggagtggcca ccgcgtcctg caacctcccc atctcggccc
     2161 tcggcgaccg gtgggtcacg accatcgagt cgcttggtga cgaagcacgg ctccacccgc
     2221 tgcagcaggc attcctcgac gagcaggccg gccaatgcgg atactgcctg acgggtgtgc
     2281 tgatgacggc tgcggcgctc ctggactcca acccggatcc aagccgtgcg gagattcgct
     2341 ccgccctgga cggtgtttta tgccgctgcg gcgtccagga ccggatggtg cgggcggtgg
     2401 aacgggccgc ggctcagctg cgcgtccagc catgacgacg gacgggctgc ccatggcgct
     2461 gcggtcgtac cccttgatcg gccagtggct ctcgatctcc ccagacgggc agatcgtggt
     2521 tcgctgcgga aaggtcgagt tcgggcaggg catccggacg gcgcaggcgc agatcgtggc
     2581 gcatgagctc gccgtgcccg tcgatgccgt gaccgtcgag gaggtgagca ccatcggcag
     2641 cccggacgag ggtgtcacct ccggcagccg ctccatcgag gaggccgatg aagggctgcg
     2701 ccgggcggca gccgagctgc gggtggctct cgcccggcgc gcagcgactc gctgtggcgt
     2761 tgcggcctcc gatctgagcg ttgattcagg gtgcgtcgtg cttcccgacg gccagcgaat
     2821 tccctatgcc gatctggtgg actcggacct ggcggccgag gcgataagcg ggaatgcctc
     2881 gccccggccg ccagctgccg gctcaccttt gggagccagc gtccctcggg tggaccttcc
     2941 cgccaaggtg ctcgggaagc cggccttcgt gggagacctc gaactccccg ggatgctcca
     3001 cggccgtgtg tgccggccgg caggcccagg cgctcggctg gtcgaagtcg atgagcaggc
     3061 cgtcctcgag atgccggggg tgcgggcagt tgttcgcgac ggcaacttcc tggcggtggt
     3121 cgctgagcgc gaggagcagg ccatccgcgc gctggcaagg ctccgccgcg tctccacctg
     3181 gaccgaaccc tccacactcc cgtcctcgac gcgcttcctg ctccatgagc aaaccttgga
     3241 tgtcacggtg gcagaggctg gagaacctcc tggcgatgcc ctccaggtgc gcccgttgac
     3301 cggcgagtac agccgtcctt acctggcaca cgcctcgctg ggcccatcct gcgcggtggc
     3361 gacgttccac ggcgactcgt acgacatctg gacccacagc cagggcgtgt accacctacg
     3421 cggggagctt tccaaggtcc tggagctgga cgcggaggag atccgcgtcc accacctcga
     3481 gggcgcgggc tgttacgggg cgaacggggc agacgacgca gctctggacg cggccctttt
     3541 agcacgcgcg gttccgggaa gccccgtgcg ggtgcagtgg atgcgtgccg acgagttcgg
     3601 gtgggagccg ttcggcacac cgatggtcgt tcggctgcag gctctgctag acgagagcgg
     3661 gtcgatcgtc gactggcagc acgacgtgtg gggccatggc caccgtgacc gggccggcag
     3721 cgacagcccg cggaacgtca ccaacctgct ggccgcgcgc cacctggcga atgcattcga
     3781 gggatcggtc cctgcgccgc cgccgacgcc gtccgcgggc agtggccgca acgcagcgcc
     3841 ggtctacgcc ttcccttccc agcgggtcat caaccactac gtgccgcggg tgcccatccg
     3901 cgtttccgcc ctgcggtcgc tgggcgccca cgccaacgtc ttcgccattg aatcgttcat
     3961 ggacgagatc gccgccgagc tcggcgtgga cccgctcgaa caccggctcc gctacctgca
     4021 cgaccagcgc gggcgtcgcg tgcttaagtc ggccaccgaa agagctgggt ggtccgggcg
     4081 ccggatcacg gaaggccgcg ggctgggcat cggattcgcg cagtacaaga acgacggctg
     4141 ctacgtggcc gtggtcgccg aggtggacgt ggacgatgag ctggtgctcc ggcgggtctg
     4201 ggcggcagtg gacgccgggc tggtcatcaa ccccgatggg ctgcgcaacc aggtcgaggg
     4261 gggcatcgct caggcggcga gctggacgct caaggagcag gtcaccttcg accggtcaca
     4321 cataacctcc cgggactggg agacgtaccc catcctacgg ttctcggagg cgccggaaat
     4381 cgaggtcgat gtgatcgtgc agcccgacga gccgccccat ggggccggcg aagcggtcgc
     4441 tgggccgacc gcgggagcca tcgcgaacgc ggtcttcgcc gcgactggtg cccgaatccg
     4501 cgacatgccc atcacccgtg agcgcctgat ccgggcgctg gcatgaccgg tgtgacgttc
     4561 gggttcaaga ccgagccgcc gcgcgcggag tgggaggacc tgcggcggat ctggcaggag
     4621 gccgatgaga tcccgctgat cgccgacctg tgggccttcg accacttcgt gccgttgcgg
     4681 gcggacgctg gcgatccgtg cctcgagggc tggaccgtgc tga
//
LOCUS       DATACA010000135         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1303592, whole genome shotgun sequence.
ACCESSION   DATACA010000135 DATACA010000000
VERSION     DATACA010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303592"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..796
                     /locus_tag="VHK28_02770"
     CDS             164..796
                     /locus_tag="VHK28_02770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2141188.1"
                     /translation="MADGCCTTDYDLIFDETSARKELTDYLDHGLDDDTSRLLDALVG
                     QDVQGSSVLEIGGGVGDVQLELLAAGVASVTDVDASQPYIAVAREEVERRGLADRVRY
                     VHGDFVAVASGVPPADVVVLNRVVCCYPDMTALVRESTARAQRVYALVLPVDRWLTKL
                     IVGLDNLRHRIGRSSFRAYVHPTAEVERLIRAAGFEPRFEHSGWMWRIRV"
     gene            complement(823..1803)
                     /locus_tag="VHK28_02775"
     CDS             complement(823..1803)
                     /locus_tag="VHK28_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX2141189.1"
                     /translation="MTRRILSIDGGGVRGVIPAVLLDALERSTGAPAREQFDFVAGTS
                     TGALVAAGVAAGVSGEELVDLYLRRGPRVFRRTPILSTLRRIVRGHMYDVRQLHRLIR
                     ETLEFHGAGDWRLDDAGLDVMFTAKGLGDSHQWYFVNDRPQANSCRTGHLSLADCLTA
                     SAAAPTYFAPWVVRGLEARGPMVDGGAGVAGNPVYQACVEAFEYSKGYQPSETIVVSL
                     GTGHYLGRRRPAWLWSWLGWLLAELLRSPGEQQTELVDRHYPQAAFYRIDVELPRDIA
                     MDDARQIAALRDIGEELAGRVDWPAVLSGEDGEWLVSRRGARPPAYAEQV"
     gene            complement(2018..2428)
                     /gene="msrB"
                     /locus_tag="VHK28_02780"
     CDS             complement(2018..2428)
                     /gene="msrB"
                     /locus_tag="VHK28_02780"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX2141190.1"
                     /translation="MTTKITKSEAEWRASLTPEQYHVLREKGTERAFTGAYWDEHTPG
                     VYRCAGCNAELFRSDTKFESGSGWPSFFTPADDAAVAVETDRSFFMTRNEVHCARCGG
                     HLGHVFDDGPDPTGLRYCINSAALKLDPEAAAER"
     gene            2522..>3331
                     /locus_tag="VHK28_02785"
     CDS             2522..>3331
                     /locus_tag="VHK28_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX2141191.1"
                     /translation="MRRAAPTALPALLAIAMVVGGCLLAEPIASGSPSLPPSVPAAAS
                     VPPESESPAATVAPEATPGADAVPRLAVGSGATTNAPGLRVRSRPGTAQRVITTMGVD
                     HDLLVGMGPVIVEGLGWYQVRDADADEPAFREGWVAAGFEPDPFLIGTAFAVEFNPYL
                     AGYAHDADGEFGPVLLPGADVTLRWIASPPTDLGCSFAVDLRPGGGQPVPAIRSTVGA
                     FPAPGEVFSDYFAGHPELIGPEVFVIVTSDCSWALTFVRPDPAASPTPSPAS"
BASE COUNT          477 a         1189 c         1178 g          487 t
ORIGIN      
        1 tgggtgccgc gctgctctgt gccgcgtggc tcccagggcg aagatccggg tgagactaag
       61 catggtggtg cggcccatca ccgccagatt agtgccgagc agcgtcgatt gcagaagtgc
      121 caaacgtcgt gatttcttgg ccgcgccgcg tagactcgac ggcatggctg acgggtgctg
      181 caccaccgac tacgacctca tcttcgacga gacatcggcg cgcaaggagc tgaccgacta
      241 cctggaccac gggctggacg atgacacctc ccggctgctc gacgccctcg tcgggcagga
      301 cgtccagggc agcagcgtgc tggagatcgg cggcggggtg ggcgacgtgc agctggagct
      361 gctggccgcg ggggtggcca gcgtgaccga cgtggatgcc tcgcagccgt acatcgcggt
      421 ggcccgcgag gaggtggagc gccgcggcct tgccgaccgg gtgaggtacg tgcatggcga
      481 cttcgtggcg gtggccagcg gcgtgccacc cgccgatgtg gtcgtgctca accgcgtggt
      541 gtgctgctac ccggacatga ccgccctggt ccgcgagtcc accgcccgag cgcagcgcgt
      601 ctacgcgctc gtcctgccgg tggaccgatg gctgacgaag ctgatcgtcg gcctcgacaa
      661 cctgcggcat cgcatcggac gcagctcctt ccgggcatac gtgcacccga ccgccgaggt
      721 ggagcgcctc atccgggccg ccggcttcga gccgcgcttc gagcacagcg gctggatgtg
      781 gcggatccgg gtctaggccc gtgccgacgg ccgctccccc gcctagacct gctccgcgta
      841 cgccggcgga cgcgcgccgc gccggctgac cagccactca ccgtcctcgc cggacaggac
      901 cgccggccag tccacgcggc cggccagctc ctcaccgatg tcgcgcagcg cggcgatctg
      961 tcgggcatcg tccatggcga tgtccctggg cagctcgacg tcgatgcggt agaaggccgc
     1021 ctgtgggtag tggcgatcga ccagctcggt ctgctgctcc ccgggcgagc gcagcagctc
     1081 cgccagcagc caccccagcc aggaccacag ccaggcgggg cggcgcctgc ctagatagtg
     1141 ccccgtgccc agcgagacga cgatggtctc cgacggctgg taccccttgc tgtactcgaa
     1201 ggcctccacg cacgcctggt agaccgggtt gccggcaacg ccggcgccgc cgtccaccat
     1261 cggtccgcgc gcttccagcc cccgaacgac ccagggcgcg aagtaggtcg gggcggccgc
     1321 cgaggcggtc aggcagtccg ccaggctgag gtggccggtg cggcaggagt tggcctgcgg
     1381 ccgatcgttg acgaagtacc actggtggct gtcgcccagg cccttggcgg tgaacatcac
     1441 gtccaggccg gcgtcgtcca gccgccagtc acccgccccg tggaactcca gggtctcgcg
     1501 aatgagccga tgcagctggc gcacgtcata catgtgcccg cgcacgatgc gccgcagggt
     1561 ggagaggatc ggggttcggc ggaagacgcg cggcccgcgt cgcaggtaga ggtccaccag
     1621 ctcctcgccg ctgactcccg ccgccacgcc ggccgccacc agcgccccgg tggaggtgcc
     1681 ggccacgaag tcgaactgct cccgtgccgg cgcgccggtg ctccgctcca acgcgtccag
     1741 cagcacggcc gggatcacgc cccgcacgcc gccaccgtcg atggagagga tgcgcctggt
     1801 cacatcggcc agcgtacccg ccggctcgac agctgcgtgt cgcgttgagc ggcgcgccgg
     1861 tcaaacggcg catgaaccgg cctccgcgct cgggatgacc gataaaccgc tcgccagggt
     1921 gcagggcccg cgccgcaaag cgccgcccac gctccccacc gggccataga ccgctcaaac
     1981 cccgcgcaat caggcgccgc cgcgccgatc gagcgaccta tcgctccgcc gcggcctccg
     2041 ggtcgagctt gagcgccgcc gagttgatgc agtagcgcag cccggtcggg tctggcccgt
     2101 cgtcgaagac gtggcccagg tgcccgccgc atcgggcgca gtggacctcg ttgcgggtca
     2161 tgaagaagga gcgatcggtc tcgaccgcca cggccgcgtc atcggccggg gtgaagaagg
     2221 acggccagcc gctgccggac tcgaacttgg tgtctgagcg gaaaagctct gcattgcagc
     2281 cggcacatcg gtacacgccg ggcgtgtgct cgtcccagta ggcgccggtg aacgcgcgct
     2341 cggtgccctt ctcgcgaagg acgtggtact gctcaggggt cagcgaggca cgccactcgg
     2401 cctcgctctt ggtgatcttg gtggtcatgg ctgaatgata cgaatctggc gtgaaggacc
     2461 gatgaagccg ccccgatgcg atacttcggc ctcctgttgc gtcctcaggg ggatgagacc
     2521 gatgcgccga gccgcaccaa ccgcccttcc tgccctccta gccatcgcca tggtggtggg
     2581 gggctgcctg ctggcggagc cgatcgcgag cggtagcccc agcctgccgc cgtcggtgcc
     2641 ggcagcggca tcggtcccac cggagagcga atcgccagcg gccacggtgg ccccggaggc
     2701 gacgccgggc gccgatgccg tgccgagact ggcggtcgga tcaggggcga ccaccaacgc
     2761 tcccggcctg cgggtgcgct cccggccggg gacggcgcag cgggtgatca ccaccatggg
     2821 cgtcgaccac gacctgctgg tgggaatggg tccggtcatc gtggaggggc tgggctggta
     2881 ccaggtccgc gacgcggatg ccgacgaacc ggcgttccgc gagggttggg tggcggccgg
     2941 gttcgagccc gatccgttcc tgatcggcac cgccttcgcc gtggagttca acccctacct
     3001 ggccggatac gcacacgatg ccgacggcga gttcggcccg gtgctgctgc cgggggccga
     3061 cgtgacgctg cgctggatcg cctcgccgcc aacagatctg ggctgctcgt tcgcggtcga
     3121 cctgcgaccg ggcggcggcc agccggtgcc ggccatccgt tccacggtcg gcgccttccc
     3181 ggccccggga gaggtcttca gcgactactt cgccgggcac ccggagctca tcggtccgga
     3241 ggtcttcgtc atcgtgacct ccgattgctc atgggcgctc accttcgtgc ggcccgaccc
     3301 cgcggcgtcg ccgacgccct cgccggcgag c
//
LOCUS       DATACA010000136         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1223953, whole genome shotgun sequence.
ACCESSION   DATACA010000136 DATACA010000000
VERSION     DATACA010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223953"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VHK28_02790"
     CDS             complement(<1..408)
                     /locus_tag="VHK28_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141192.1"
                     /translation="MSGIEVQAGTVQVWSDLGCPWSHAVVWRLWDARRRLGLEERVRF
                     DHHVFPLELFNNEPTPKAHRDAEWPVASQLAPRAGWQAWSEPLETYPVTMLPPMEAVQ
                     AAKLQSLAAAEELDRGLRRAFWGESRCISLRHVI"
     gene            complement(435..1322)
                     /gene="deoC"
                     /locus_tag="VHK28_02795"
     CDS             complement(435..1322)
                     /gene="deoC"
                     /locus_tag="VHK28_02795"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2141193.1"
                     /translation="MLGRVWPVDQVGVQERAASLARRSIKRDAKLWALDLAIRMMDLT
                     TLEGKDTPGKVRALAGKARRPDPTDPSIPSVAALCVYPSLVPIAKEALEGSTVKVASV
                     ATYFPSGQAPLEVKLHDVRQAIELGADEIDMVIDRGAFLAGEYARVYDEIVAVKEACG
                     EHHLKVILETGELETFDNVRRASVLAMAAGADFIKTSTGKVNPAAELPVTLVMLEAIR
                     DFYQQTGRAVGMKPAGGIRTSKEAISYLVVLYETLGPDWMTPDRFRFGASSLLNDVLM
                     QIRKERTGRYSGGDYFTLD"
     gene            1434..>1781
                     /locus_tag="VHK28_02800"
     CDS             1434..>1781
                     /locus_tag="VHK28_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141194.1"
                     /translation="MNAPRETLLYCLTRDRELAAAIDGRLSDALSFFYNDAARLHQAI
                     VLRAPDAVVVDTGAIREEYGDAGLGPVIAFIRRRTPEARIAVRPHAGGEWLVAAEAGE
                     GVRMLPPEAGACVE"
BASE COUNT          243 a          619 c          636 g          283 t
ORIGIN      
        1 gatcacgtgc cgcaggctga tgcagcggct ctcgccccag aacgcgcggc gcagcccgcg
       61 gtccagctcc tcggccgcgg ccagggactg cagcttggcg gcctgcacgg cctccatggg
      121 cggcagcatg gtgaccgggt acgtctcgag tggttcgctc caggcctgcc acccggcacg
      181 tggcgccagc tggctggcca ccggccactc ggcgtcccgg tgagccttgg gcgtgggctc
      241 gttgttgaac agctcgaggg gaaagacgtg atggtcgaag cggacgcgtt cctcgaggcc
      301 gaggcggcgg cgggcgtccc agagccgcca caccacggcg tgcgaccacg ggcagccaag
      361 gtctgaccag acctggaccg ttcctgcctg cacctcaatc ccgctcatgg ctacgactct
      421 agccccggca ggcctcagtc gagggtgaaa tagtcgccgc ccgagtagcg gccggtgcgc
      481 tccttgcgga tctgcatcag cacgtcgttg agcaggctgc tggcaccgaa gcggaagcga
      541 tccggtgtca tccagtccgg tccgagcgtc tcgtacagca ccaccaggta gctgatcgcc
      601 tccttcgagg tgcggatgcc gcccgccggc ttcatgccca cggcccggcc ggtctgctgg
      661 tagaagtcac ggatcgcctc cagcatgacc agggtgaccg gcagctcggc ggccgggttc
      721 accttgccgg tgctggtctt gatgaagtcg gcaccggccg ccatggccag gaccgatgcc
      781 ctgcgcacgt tgtcgaacgt ctccagctcg cccgtctcca ggatcacttt gaggtggtgc
      841 tctccgcagg cttccttgac ggcgacgatc tcgtcgtaga cgcgcgcgta ctcccccgcc
      901 aggaaggcgc cgcgatcgat caccatgtcg atctcgtccg cgcccagctc gatcgcctgg
      961 cgcacgtcgt ggagcttgac ctccagcggt gcctggccgg atgggaagta ggtggccacc
     1021 gacgccacct tgaccgtgga gccttcgagc gcctctttgg cgatcggcac caggctgggg
     1081 tacacgcaca gcgccgccac gctcgggatg gacgggtcgg tcgggtccgg ccgccgcgcc
     1141 ttgcccgcca gggcgcggac cttgccgggt gtgtcctttc cctcgagggt ggtgaggtcc
     1201 atcatccgga tggcgagatc gagggcccag agcttcgcgt cgcgcttgat gctgcgccgc
     1261 gccaggctgg ccgcccgctc ctggacgccg acctggtcga ccggccacac gcgcccgagc
     1321 atgctgccca gcgcctggtg gcgcggcgcg gagaggtcgc gggcggggtc gcggcgggcg
     1381 ggcgtggtgg tggccatgga cctcgaattg tactgctacg cttcgcgagc cccatgaatg
     1441 ccccccggga aacgctgctc tactgcctga cccgagaccg ggagctggcc gccgccatcg
     1501 acggccggct gagcgacgcg ctcagcttct tctacaacga cgcggcgcgc ctgcaccagg
     1561 cgatcgtcct ccgggctccg gatgcggtgg tggtcgacac cggggccatt cgcgaggagt
     1621 acggggatgc cggcctggga ccggtgatcg ccttcatccg tcggcggacg ccggaggcgc
     1681 gcatcgcggt gcggccgcat gccggcggag agtggctggt ggcggccgag gccggcgagg
     1741 gcgtccgcat gctccctccg gaggcaggcg cgtgcgtgga g
//
LOCUS       DATACA010000137         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1705723, whole genome shotgun sequence.
ACCESSION   DATACA010000137 DATACA010000000
VERSION     DATACA010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705723"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..317
                     /locus_tag="VHK28_02805"
     CDS             114..317
                     /locus_tag="VHK28_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2141195.1"
                     /translation="MSVARVTELSCTSNESFEDAIRQGIERAQRTLRQVRSAWIKEQR
                     VNIGSNGAMEFQVNILVTFVLED"
     gene            complement(330..1247)
                     /locus_tag="VHK28_02810"
     CDS             complement(330..1247)
                     /locus_tag="VHK28_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2141196.1"
                     /translation="MNGAEPATPLGRAGVWSNFDRLPIDEVIAFARAAEERGYGAFWT
                     QETAGRDPFALLGHLAARTERIRLGVGIAVIYGRDAVAMRGGAATVHELSAGRMVLGL
                     GASHRDTVSQVRGHQYRGPLTVMRDYLDAYDAASYRGPAPHGSPTLLLAALRQRMLAL
                     AAARADGAFPYLVPLHYVSTARRILDEATAATGRPPALLVVSQACVLDADPVAARRAA
                     RRYLERYLGLPNYLNNLLELGYDEAELQPPGADRVVDDLVAWGDVTAIQARLRAMLDA
                     GADHVAVIPLTPEGLMADRHTMEALAPPW"
     gene            complement(1249..2061)
                     /locus_tag="VHK28_02815"
     CDS             complement(1249..2061)
                     /locus_tag="VHK28_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141197.1"
                     /translation="MSYERRPDRRPGGQRGGFGRPDRPQRDRPPRDRMDRDRPPPDRD
                     RGPRDRSRPYTPRPPDEGGMAIRLDPRRLSALKLLAAEAGVRPGELVTQWVQERLDAA
                     RGGRPPSATETADPGALAALAARVDQLAQRLDELESQRATAGDGTAPAPTAQAAQAAA
                     TPEDTSQERTPAAPSQPAEKPKRAARQRKSAREATGPRVPLHEEIAAVINEQGPMTAG
                     ELATAIRERGRYAPPRSAKPLDAATVNARVSNPVYRSRFVRSEGRIGLANAS"
     gene            complement(2134..3858)
                     /locus_tag="VHK28_02820"
     CDS             complement(2134..3858)
                     /locus_tag="VHK28_02820"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX2141198.1"
                     /translation="MIRWTFSTIAAVVLVVGLLVVPRVLDITGVSATRFPDPVRDQAV
                     YDPAGAVTPGVETAIEEMIDRIEERSGAEMVVYVRVEPSNDESNLSDARALIDQWGIG
                     RAGYDDGFVILLSFNDTSFQHGVLSTYAGGGFKAVYLPESEQQVIRDEVVIPAIRQGS
                     PEGGLAAAVSLVEAAITSDKTGRLEFFRIVNAMVGIPGGILALLLTLGSAYVTWRRYG
                     DDPELVDSPSILMAGPPAGMTAPLATVIRQGRATQHSQNTLLVDLASRGLIRFRNLDR
                     VGKVKSDDDPDPLTDPAIEVFKRPPGTGRLDPTLTDAFQIVQQQELGGVLARERLWKL
                     NERLGGMKQRLEEEAVRLGWLSQRPSPMISRWTWIGVAEIVGGIGLAWLGYTIPMSGL
                     TLLGAAFGVGGIGTVGFGSAMSQRTRKGAYVDGMLKAYRRTLRKTLEQARNMGEVVTV
                     PEVAVLADTPDKAVLWGIALGLHDEVATVLERGLADPGVQADPSRAYYPTWLGTSSSS
                     NLSSSSNVAAGAGLGGLFSGSGTPDIGGMFGALGSIGSTPASSSSGSGGGFGGGGSSG
                     GGGGSSSF"
     gene            4093..>4289
                     /locus_tag="VHK28_02825"
     CDS             4093..>4289
                     /locus_tag="VHK28_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141199.1"
                     /translation="MPRSFTALVAALVLCLLLVPALPVHPRASAMELPDYHAWPSATE
                     QYVLAARRVPATPAPSPVPTAA"
BASE COUNT          589 a         1564 c         1523 g          613 t
ORIGIN      
        1 ggcctggacg ggctcgcacc agtcctagcc cagccgcatc ggggcaaagg cgcgagttgc
       61 cggcgagaat catgggtcca tacttggccg tccatcacga caggaggaga ccgatgtccg
      121 tcgcgcgcgt caccgagctc agctgcacgt ccaacgagtc cttcgaggac gccatccgcc
      181 aggggatcga gcgggctcag cgcaccctgc gccaggtgcg gagcgcctgg atcaaggagc
      241 agcgggtgaa catcggctcc aacggcgcca tggagttcca ggtcaacatc ctggtcacct
      301 tcgtcctcga ggactgaggg cagccactcc taccaggggg gagccagggc ctccatggtg
      361 tggcggtcgg ccatcaggcc ctctggggtg agcgggatga cggccacgtg gtcggccccg
      421 gcatccagca tggcgcgcag ccgagcctgg atggccgtta cgtcgcccca ggccaccagg
      481 tcgtcgacca cccggtcggc accgggaggc tgcagctccg cctcgtcgta gccgagctcg
      541 agcaggttgt tcaggtagtt cggcaggcca aggtagcgct ccaggtagcg ccgcgccgcc
      601 cggcgggcgg cgaccgggtc ggcgtccagc acgcaggcct ggctcaccac cagcagcgcg
      661 ggcggccgcc ccgtggccgc ggtcgcctcg tccagaatgc gccgcgccgt gctcacgtaa
      721 tggagcggga ccaggtaggg gaacgcgcca tcggcccggg cggcggccag ggccagcatg
      781 cgctgccgca gcgcggccag cagcagcgtc gggctgccgt gcggcgccgg gccgcggtag
      841 ctggcggcgt cgtaggcgtc caggtagtcg cgcatcacgg tcagcgggcc gcggtactgg
      901 tggccgcgca cctggctgac ggtgtcgcgg tggctggcgc caagtcccag aaccatgcgg
      961 ccagcggaca gctcgtgcac ggttgcggcc ccgccgcgca tggcgaccgc gtcccgcccg
     1021 tagatcacgg cgatccccac gcccagcctg atccgctccg tccgggccgc caggtggccc
     1081 agcagggcga aggggtcgcg gccggcggtc tcctgcgtcc agaaggcgcc gtagccgcgc
     1141 tcctcggctg cccgcgcgaa ggcgatcacc tcgtcgatcg gcaggcggtc gaagttggac
     1201 cacaccccgg cccggccgag gggcgtggcc ggctcggccc cgttcacgct acgaggcgtt
     1261 ggcgagcccg atgcggccct cggagcgcac gaatcgcgac cggtacaccg ggttcgacac
     1321 gcgcgcgttg acggtggcgg cgtccaacgg cttcgcggaa cgcggcggcg cataacggcc
     1381 ccgctcacgg atggcggtgg ccagctctcc cgcggtcatg ggaccctgct cattgatgac
     1441 cgccgcgatc tcctcgtgca gcgggacccg cggtccggtc gcctctcgag ccgactttcg
     1501 ctgcctggca gcacgcttcg gcttctcggc cggctgggat ggcgcggcgg gggtccgctc
     1561 ctgtgacgtg tcctccgggg tggccgcagc ctgggctgcc tgcgcggtcg gcgccggcgc
     1621 cgtgccgtcg ccggcggtgg cacgctggct ctccagctcg tccaggcgct gcgccagctg
     1681 gtccacgcgg gcggccaggg cggcgagcgc acccggatcg gcggtctcgg tcgccgacgg
     1741 cggcctgccg ccacgcgccg catccagtcg ctcctggacc cactgggtca ccagctcgcc
     1801 agggcgcact cctgcctcgg cggccagcag cttgagggcc gagagccggc gggggtccag
     1861 gcggatggcc atgccaccct cgtcgggcgg tcgaggcgtg tagggacggg accggtcgcg
     1921 cgggccgcgg tcgcgatccg ggggcggccg atcgcgatcc atccggtcgc gcggcgggcg
     1981 gtcgcgctgt ggccggtccg gccggccgaa gccgccgcgc tggccgccgg gacggcggtc
     2041 gggtcgtcgt tcgtatgaca tggacgctcc actctagacg ttcgcatcgg tctacgccag
     2101 atgcagaacc gggcgttcag gaccgcaggc gggtcagaaa gagctgctgc cgccgcctcc
     2161 gcctgagctg ccgccgccac cgaagcctcc ccccgacccg gaggagctgg acgctggggt
     2221 tgagccgatg ctgcccagcg cgccgaacat gccgccgatg tccggtgtgc ccgacccgga
     2281 gaacaggccg cccagcccgg ctccggcggc cacgttcgag ctggacgaca ggttggacga
     2341 ggacgaggtg cccagccagg tcgggtagta ggcgcgggac gggtcggcct gcacgccggg
     2401 atcggccagg ccccgctcca ggacggtggc cacctcgtcg tggaggccca gggcgatgcc
     2461 ccacaggacg gccttgtcgg gagtgtcggc cagcacggcc acctccggca cggtgaccac
     2521 ctcgcccatg ttgcgtgcct gctccagcgt cttgcgcagc gtgcgccggt aggccttgag
     2581 catgccgtcc acgtaggctc ccttgcgcgt gcgctggctc atggcggaac cgaatccgac
     2641 ggtaccgatg ccgcccacgc caaatgccgc gcccagcagg gtgagtccgc tcatggggat
     2701 ggtgtatccc agccaggcga gaccgatgcc gcccacgatc tcggccacgc cgatccaggt
     2761 ccagcgcgag atcatcgggc tcggccgctg gctcagccag cccagccgca cggcctcctc
     2821 ctccagtcgc tgcttcatgc cccccagccg ctcgttcagc ttccacagcc gttcgcgggc
     2881 caggacacca cccagctcct gctgctggac gatctggaag gcatcggtca gggtcggatc
     2941 caggcgaccg gtgccgggcg gccgtttgaa gacctcgatg gccgggtcgg tcagcggatc
     3001 cgggtcgtcg tcactcttca ccttccccac gcggtccagg ttgcggaagc ggatcaggcc
     3061 acggctggcc aggtcaacca gcagcgtgtt ctggctgtgc tgcgtggctc ggccctgacg
     3121 gatcacggtg gccaggggag ccgtcatgcc cgccggcggg ccggccatca ggatcgaggg
     3181 tgagtccacg agctcgggat cgtcgccgta gcgacgccag gtgacatacg cggaccccag
     3241 ggtgagaagc agggccagga tgccacccgg gatgccgacc atggcgttca cgatccggaa
     3301 gaactccagg cgccccgtct tgtcggaggt gatcgcggct tcgaccaggg acacggcggc
     3361 ggccaggccg ccctcgggcg agccctggcg gatggccggg atgaccacct cgtcgcgaat
     3421 cacctgctgc tcgctctccg gcaggtacac cgccttgaac ccgccaccgg cgtaggtgct
     3481 caggacgccg tgctggaacg aggtgtcgtt gaagctgagc aggatgacga agccgtcgtc
     3541 gtagccggcg cggccgatgc cccactggtc gatcagtgcc cgcgcgtccg acaggttgct
     3601 ctcgtcgttg gagggctcga cgcgcacgta gacgaccatc tcggcgccag agcgctcctc
     3661 gatccggtcg atcatctcct cgatggcggt ctccacgccg ggagtgaccg cgccggccgg
     3721 gtcgtagacc gcctggtccc ggaccgggtc ggggaagcgc gtggcgctga ctccggtgat
     3781 gtccagcacc cgcggcacga caagcaggcc gaccaccagc accaccgccg cgatggtcga
     3841 gaaggtccag cgaatcacgc cgcgcaggat acgccggagc ggccgttcgc ggtctcggcc
     3901 cgtgcctgtt agggtgacgt ggcacacgtc gtgctcaatc gaccgtccag cgcgtggttc
     3961 agtgcccgag ccgcgcgggc cgttcacgga cgtccttcgg cctgccccag gttgcagcgc
     4021 cgctcgagcc tctcttcgga ccgtcctcca ggcccggcca cgccctgcct aacctgcatg
     4081 gagcgactca tattgccccg atccttcacg gcactcgttg ccgcccttgt cctgtgcctg
     4141 ctgctcgtcc cggccctgcc ggttcacccg cgggcctccg ccatggagct tcccgactac
     4201 cacgcctggc cgagcgccac cgagcagtac gtgctcgccg cccgccgggt tcccgccaca
     4261 cccgccccga gcccggttcc caccgccgc
//
LOCUS       DATACA010000138         3001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1464657, whole genome shotgun sequence.
ACCESSION   DATACA010000138 DATACA010000000
VERSION     DATACA010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464657"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VHK28_02830"
     CDS             complement(<1..708)
                     /locus_tag="VHK28_02830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2141200.1"
                     /translation="MKRSADHAEVVIVGGAIVGSAVAAFLAMRPDAPRRIVVVERDPT
                     YRTSSTTLSAASIRQQFSTAVNIEISRFGISVIKDLDRWLGVPGDPPLDIDFVEGGYL
                     FLATDAGLATLEHNHALQREHDAPVALLGPMELRQRFPWLNVEDIAAGSLGLADEGWF
                     DAHALLQAFRRRARSLGVEYVTDEVTSLECDRSRITAARLANGGSIGLDWVVDAAGPR
                     AAEVAALAGLDLPVRPRK"
     gene            761..2440
                     /locus_tag="VHK28_02835"
     CDS             761..2440
                     /locus_tag="VHK28_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2141201.1"
                     /translation="MAGPKPVILTVDDDVPVLRAVERDLRSRYGKDYRIVAASSGAEA
                     LDAVRQLSVRGNNVALFVVDQRMPKMTGIEFLAEAIPLQPDAKRVLLTAYADTDAAIQ
                     AINQIRLDHYVLKPWDPPEERFYPVIDELLADWSATFRPPFEGLRLLAGRWAASGHLI
                     RDFLTRNQVPYKWLDPTEDDEGRRLVASLPPAAAQGISEDPSAPMVLFPDGTVLRDPS
                     TRDIAERVGLQTRAALPFYDLVIVGGGPAGLAAAVYGASEGLKTLLVEREAPGGQAGT
                     TSRIENYLGFPAGLTGGDLARRALAQARRLGAEILSPQDVVGIRRDDPYRVVMLADGT
                     ELSCQTVVVATGVSYRQLDLPRAAELAGAGIYYGAATTEAVLYRDTDVGVLGGGNSAG
                     QAAVHLSRYARRVFLFIRGATNSGMSQYLIDQMAAIENIEVRLSCQISEVRGDTHLSG
                     VTTQGPQGEERLELAAIFIFIGQQPRTDWLEGMVQRDSGGFVVTGPALLEDGKPPVGW
                     GLNREPFLLEASVPGIFAAGDVRHRSIKRIASAVGEGAMAVQFVHQYLATP"
     gene            2449..>3001
                     /locus_tag="VHK28_02840"
     CDS             2449..>3001
                     /locus_tag="VHK28_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2141202.1"
                     /translation="MTIEALRAAPLFTGLADDDLARLAATVERMRLAPGEYLVREGDA
                     GDAMFVVVSGELEVTKRAGATEQPVAVVGPGAIQGEIGAISGASRAASVRAMTDVEVL
                     RVPREGLLALLDAGPEPALAMLRTVLERIRGLEMFAREREKLAALGTLAAGLAHELNN
                     PAAAIRRSVSALAQAIAERDRLHP"
BASE COUNT          434 a         1069 c         1080 g          418 t
ORIGIN      
        1 cttgcgcggt cgcaccggca ggtccaggcc ggccaaggca gccacctccg ccgcgcgcgg
       61 tccggccgcg tcgaccaccc aatccaggcc gatggagccg ccgttcgcca gccgcgccgc
      121 cgtgatccgg gaccggtcgc actcgagtga cgtgacctcg tcggtgacgt actccacgcc
      181 gagcgagcgg gcgcgccgcc ggaaggcctg gaggagggcg tgggcgtcga accacccctc
      241 gtcggcgagc cccagcgacc cggccgcaat gtcctccacg ttgagccacg ggaagcgctg
      301 gcgcagctcc atcggtccga gcagggcgac cggcgcgtcg tgctcgcgct gcagggcatg
      361 gttgtgctcc agggtggcga ggccggcatc ggtcgccagg aagagatagc caccctcgac
      421 gaagtcgatg tccagcggcg ggtcgccggg aacgccgagc catcggtcca gatccttgat
      481 gaccgatatc ccaaagcggc tgatctcgat gttgaccgcc gtgctgaact gctgccggat
      541 ggacgccgcg gacagggtgg tggacgaggt gcggtaggtc ggatctcgct ccacgaccac
      601 gatacggcgg ggggcgtccg gccgcatggc caggaaagcc gccaccgcgc tgcccacgat
      661 ggcccctccc acgatgacca cctccgcatg gtcagcagag cgcttcaccg gccgatgcta
      721 ccggtgcctg gcctcctgcg agcggtagac tcggcagcgc atggccggcc cgaagcccgt
      781 catcctgacg gtagacgacg acgttcccgt ccttcgtgcc gtggagcggg acctccgctc
      841 gcggtacggc aaggactacc gcatcgtggc cgcctcgtcc ggcgccgagg cgctggacgc
      901 ggtccgccag ctgagcgtgc gcggcaacaa cgtcgcgctg ttcgtggtcg accagcgcat
      961 gccgaagatg acgggcatcg agttcctggc cgaggcgatt ccgctgcagc cggacgccaa
     1021 acgggtcctg ctgacggcct acgcggacac cgatgccgcc atccaggcca tcaaccagat
     1081 ccgcctggac cactacgtcc ttaagccgtg ggacccgccg gaggagcgtt tctacccagt
     1141 catcgacgag ctgctggccg actggagcgc caccttccgc ccgccgttcg agggcttgcg
     1201 gctgctcgcc gggcgctggg cggcaagcgg gcacctgatc cgcgacttcc tgacccgcaa
     1261 ccaggtgccg tacaagtggc tcgaccccac cgaggacgac gaggggcggc ggctggtggc
     1321 ctccctgccg ccggccgcgg cgcagggcat ctccgaggat ccctcggccc cgatggtgct
     1381 ctttccggac ggcacggtgt tgcgcgaccc gagcacccga gacatcgccg agcgggtggg
     1441 cctgcagacg cgggccgcac tgccgttcta cgacctggtg atcgtgggcg gcggtccggc
     1501 cgggctggcg gccgcggtct acggggccag cgaggggctc aagacgctgc tcgtggagcg
     1561 cgaggctccc ggtggccagg cggggaccac cagccgtatc gagaactacc tcgggtttcc
     1621 ggccggcctg accggtggcg acctggcgcg ccgggcgctg gcccaggcgc ggcggctggg
     1681 cgcagagatc ctctcgccgc aggacgtggt cggcatccgc cgtgacgacc cgtaccgggt
     1741 ggtgatgctc gccgacggca ccgagctctc ctgccagaca gtggtggtgg ccaccggcgt
     1801 cagctaccga cagctggacc tgccgcgcgc tgccgagctg gccggcgcgg gcatctacta
     1861 cggcgccgcc accaccgaag cggtgctgta ccgcgacacc gacgtgggcg tcctcggcgg
     1921 cggcaactct gccggacagg ccgccgtcca cctgtcgcgc tacgcgcgcc gcgtgttcct
     1981 cttcatccgc ggggccacca actcggggat gagccagtac ctgatcgacc agatggcggc
     2041 catcgagaac atcgaggttc ggctcagctg ccagatcagc gaggtgcgcg gcgatacgca
     2101 cctgtcgggc gtcacgaccc aggggccgca gggggaggaa cgactcgagc tggcagcgat
     2161 cttcatcttc atcgggcagc agccgcgcac cgactggctg gagggcatgg tgcagcgcga
     2221 ctccggcggc ttcgtagtga ccgggccggc gctgctggag gacggcaagc ccccggtggg
     2281 atggggcctg aaccgcgagc cgttcctcct ggaggccagc gtgccgggga tcttcgccgc
     2341 cggcgacgtc cggcatcggt ccatcaagcg cattgccagc gcggtgggcg agggcgccat
     2401 ggccgtccag ttcgtgcacc agtacctggc cacgccctag gtggaccgat gaccatcgag
     2461 gccctgcgcg cagctccgct cttcaccggc ctggccgatg atgacctggc gcgcctggca
     2521 gccaccgtcg agcgcatgcg cctggcgccc ggcgagtacc tggtacggga gggcgacgcg
     2581 ggcgacgcca tgttcgtggt ggtctccggc gagctggagg tgaccaagcg ggcgggagcc
     2641 accgagcagc ccgtggccgt ggttggcccc ggcgccatcc agggcgagat cggcgccatc
     2701 tccggggcgt cgcgtgccgc ttcggtgcga gcgatgaccg acgtggaagt cctgcgcgtg
     2761 ccccgcgagg gcctgctggc actgctcgac gcagggccgg agccggccct ggccatgctg
     2821 cgcacggtgc tcgagcgaat ccgtggcctg gagatgttcg cccgtgagcg ggagaagctg
     2881 gccgccctgg gcacgcttgc cgccgggctc gcccacgagc tcaacaaccc ggcggccgcc
     2941 atccgccgat ccgtctccgc gctggcgcag gccattgccg agcgggaccg attgcacccg
     3001 c
//
LOCUS       DATACA010000139         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_260352, whole genome shotgun sequence.
ACCESSION   DATACA010000139 DATACA010000000
VERSION     DATACA010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260352"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /gene="murG"
                     /locus_tag="VHK28_02845"
     CDS             complement(<1..1012)
                     /gene="murG"
                     /locus_tag="VHK28_02845"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005270072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX2141203.1"
                     /translation="MKTHEPSVVLAGGGTAGHISPLLAIAGAIREQAPDAGILAVGTA
                     AGMETRLVPAAGYELATIERVPMPRRPSLDLLKLPFRFGTAVKQARRIIESAGADVVV
                     GVGGYVSTPMYIAAKLAKVPLVIHEANARPGLANRVGARLTDDVAVAFRSTKLPGATW
                     VGMPMRREISALDRSAERAAARDRLGLEQDKPTLIVTGGSSGAASINRTIRASLEKFA
                     AAGIQTLHITGKGKELREPDGSLVRVPGYHQVEFVDGMENVYAAADLLLARAGAATVC
                     EIAAVGLPAVLVPLPHGNGEQVLNAADLVGAGGAVMVKDADFTPDWATAQLPALLQDH
                     NKL"
     gene            complement(1038..>1886)
                     /locus_tag="VHK28_02850"
     CDS             complement(1038..>1886)
                     /locus_tag="VHK28_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2141204.1"
                     /translation="PSEAAKLALGLWMACVLVRKGPLLSEWKHTVVPVLPVGGLVILL
                     VLLGNDLGTAMIIMLIIAGALYFAGARVKLFLVAGGAAVAGVLLLAITSPNRVARITM
                     WLDQSCEVTTGLNMQSCNGLFALASGGWWGVGLGQSRQKYSWIPEAHNDYIFAIIGEE
                     LGLLGTLVVVALFGILAVAIVRTVMRHDDAFVRIFGGSIMVWIIGQAFLNMGVVTGLL
                     PVVGVPLPFISYGGSALTITLAAVGVLLSFARPDARAGRNRARKRARNLAQRKPLDPA
                     GNISNQ"
BASE COUNT          359 a          632 c          582 g          313 t
ORIGIN      
        1 gcagcttgtt atggtcctgc agcagcgcag gaagctgcgc agttgcccag tcaggcgtga
       61 agtcggcgtc cttgaccatc accgctcccc cggctccgac taggtccgcg gcattcagta
      121 cctgctcgcc gttgccgtgc ggcagcggca caagaacagc cggcaggccg acggcggcga
      181 tctcgcagac agtagctgcg ccggccctgg ccagcagcag atccgctgca gcgtagacgt
      241 tttccatgcc gtccacgaat tcgacctggt gatagccggg aaccctgacc aggctgccgt
      301 cgggctcgcg gagctctttc cctttgcccg tgatgtgcag ggtctggatg cctgccgccg
      361 cgaacttctc caacgacgcc cggatggtgc ggttgatgct ggcggcgccg gaggagccac
      421 cagtcacgat cagcgttggc ttgtcctgtt ccaggcccag ccggtccctg gccgcggcac
      481 gctcggccga tcggtccagt gccgagattt cccggcgcat gggcatgcct acccaggtgg
      541 cgccaggcag tttggtactc cggaaagcca ccgcgacgtc gtcggtcagc cgcgccccga
      601 cgcggttggc caagcccggg cgcgcgttag cctcatggat caccagcggc acctttgcca
      661 gcttggcggc aatgtacatg ggagtgctga cgtacccgcc gacaccgacg acgacatccg
      721 cccccgcgct ttcaatgatg cggcgggcct gcttcaccgc tgtgccgaac cggaacggaa
      781 gtttcagcag atccagcgaa ggacgccgtg gcatcggaac ccgctcgatc gtggccaact
      841 catagccggc agcaggaacc agcctggttt ccatgcctgc agccgtaccg acggccagaa
      901 tcccggcgtc cggtgcctgc tcccggatgg ctcccgcgat ggccagcaga gggctgatgt
      961 gtccggcggt gccgccgccg gcgagaacca cggacggttc atgcgtcttc ataaagtact
     1021 ggtaccttct gcctcggtca ttggttgctg atgttgccgg ccggatccag cggcttccgc
     1081 tgggccaggt tcctggcgcg tttcctggca cggttcctgc cggcccgggc atcaggacgg
     1141 gcaaaggata acagtacccc gaccgcggca agtgtgatgg tcagtgccga gccgccgtag
     1201 gaaatgaacg gcagcggaac accaacgacg ggcagcagtc cggtcaccac ccccatgttc
     1261 aggaaagcct ggccaatgat ccacaccata atggaaccgc cgaaaatgcg gacaaaggca
     1321 tcgtcatggc gcatgacggt ccgcacgatc gctacggcca gaataccgaa aagggccacc
     1381 acaacgaggg taccgagcaa gcccagttcc tcaccgatga tcgcgaaaat gtagtcgtta
     1441 tgtgcctcgg gaatccagct gtacttctgc cggctctggc ccaggcccac gccccaccag
     1501 ccgcccgacg ccagcgcgaa cagtccattg caggactgca tgttcagacc ggtggtcacc
     1561 tcgcagctct gatccagcca catggtaatg cgcgccaccc ggttaggact cgtgatcgca
     1621 agcagcagca cgcccgctac cgctgctccg ccggcaacga ggaagagttt tacccgggct
     1681 ccggcgaagt aaagtgcgcc ggcgatgatg agcatgatga tcatggccgt gcccaggtca
     1741 ttgccaagaa gcaccagcag aatgaccagt ccaccgaccg gcagtacagg caccacggtg
     1801 tgcttccact cgctgagcaa cgggcccttg cggaccagga cacaggccat ccaaagaccc
     1861 agtgcaagtt tggcggcctc ggatgg
//
LOCUS       DATACA010000140         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_983776, whole genome shotgun sequence.
ACCESSION   DATACA010000140 DATACA010000000
VERSION     DATACA010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983776"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VHK28_02855"
     CDS             <1..579
                     /locus_tag="VHK28_02855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX2141205.1"
                     /translation="GEADPPVSLVTLDIGGNDLLRLLTADPCASQPDGAACQGQVRGT
                     LASFEVNLRRVLDELRAALDVHAPDAPLLVMTYFNPFSGTDAIYERGGEVALLGEDLT
                     IDCQAAGDPAMRGMNDIILCVAGEAGAIGVDVHPRFTALGLELTHIGAQDIHANDRGY
                     EVIAAEMLAELDRHTSPASAGRSAIRSALHLQ"
     gene            588..1364
                     /locus_tag="VHK28_02860"
     CDS             588..1364
                     /locus_tag="VHK28_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2141206.1"
                     /translation="MRLAAMGDIHCTRDSHGELAETFAAAGEAADLLVLCGDLTDYGL
                     PAEAEVLGQELASAKVPVVGVLGNHDHESGEAGEVARILADAGVRLLDGDAVEIDGVG
                     FAGTKGFGGGFGRHALGSWGEPAIKAFVQEAVDEALKLENALARLRTDLRVAVLHYAP
                     IRDTVENEPPEIFPWLGSERLEEPIDRFELTLALHGHAHHGAPEGRTRGGVPVYNVSA
                     PLLRGLDEQRPFRVIELPAQPAAAGPRVAASALDRQSQGL"
     gene            1369..2148
                     /locus_tag="VHK28_02865"
     CDS             1369..2148
                     /locus_tag="VHK28_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141207.1"
                     /translation="MGGRTPTAANLDPRAVAFYRHAIEALREASVPFLVGGAYALEAH
                     AGISGRTKDLDLFLRPSDVERALRALAQSGYRTELTYPTWLAKAYMGDDFVDLIFSSG
                     NGLGLVDDDWFANPRRIGVLGYKLPLVPIEEIIWQKAFIMERHRYDGADVLHLMRAAD
                     GHLDADRLLDRFGMHWRVLLAHVILFDYVYPAERDLLPDRLRAELLRRAKEETDAQVA
                     VAGTNGGGAKLCWGTFLSSYEYVVDIEQFGMADARQSLRKD"
     gene            complement(2145..2510)
                     /locus_tag="VHK28_02870"
     CDS             complement(2145..2510)
                     /locus_tag="VHK28_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141208.1"
                     /translation="MIWRWSLAALLLIHGGIHLLGFLASTGLAVVPQISAEPTLLPDD
                     YEVGDQVLVALGALWLVAFLGFAASAIGLGMRRSWWLRLAVASAVLSTFLIVLWWPDA
                     AFGLVPNLMVFVVFAVRRF"
     gene            complement(2507..3217)
                     /locus_tag="VHK28_02875"
     CDS             complement(2507..3217)
                     /locus_tag="VHK28_02875"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX2141209.1"
                     /translation="MDIELLPEPVRRYLDVGFGGSAPSPRTLVMEGTGRVRIGPLPWL
                     PIEVQMNHEPGRNYVSLIRLRPGGVPVLRVVDAYVDGRGITKIGPFASVGEEVDQGAF
                     LAMWAGAIGLPSAWADGVAWEAVDAQTARARLPFSRDAEVATVHFDPTSGFPVRFEAD
                     RYRTRGGPKIRWFGGSRAWQVAGGLPYPSRVDALWADEPAPWFEMRVERARIDVPVEA
                     AMGVGRRAIAQAAGEERS"
     gene            3273..>4150
                     /gene="pyk"
                     /locus_tag="VHK28_02880"
     CDS             3273..>4150
                     /gene="pyk"
                     /locus_tag="VHK28_02880"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX2141210.1"
                     /translation="MRRTKLVCTIGPASQDRVDQLVAAGMDVARLNFSHGTDEDHQRA
                     AAAVRAASERQGRPVAILADLPGPKIRLGDLPHGELALVTGERFALGEDASVTYAGLA
                     DDMRNGDRLMLADGAVELEVLGTRAGIVETQVVRGGTVRSGAGLNGPSGRLRLPAVTD
                     RDHEALRTILGMGVDYVAQSFVRHADDLRAVRELMGDRPMPIMAKIETRAAVENLGAI
                     LEETDALMLARGDLGVEIPFEDVPLIQKRAIDASVAAGVPVVVATQMLESMVSAPRPT
                     RAEANDVANAVLDGADA"
BASE COUNT          645 a         1465 c         1474 g          566 t
ORIGIN      
        1 ggcgaggctg accctccggt cagcctggtc acgctggaca tcggcgggaa cgacctgctg
       61 cgcctgctga ctgccgatcc ctgcgcttcc cagccggacg gtgccgcctg ccagggacag
      121 gtgcgcggca ccctggcgtc cttcgaggtg aacctgcgca gagtcctgga cgagctgcgc
      181 gccgccctcg acgtccatgc cccggacgcg ccactgctgg tgatgaccta cttcaacccg
      241 ttctccggaa ccgatgccat ctacgaacgg ggtggcgagg tggccctgct gggggaggac
      301 ctcacgatcg actgccaggc ggctggtgat ccggccatgc gcggcatgaa cgacatcatc
      361 ctgtgcgtgg ccggagaggc cggcgccatc ggcgtagacg tccatcccag gttcacggcg
      421 ctgggcctgg aactgaccca catcggtgcc caggacatcc acgccaacga tcgcggatac
      481 gaggtgatcg ccgccgagat gctggccgag ctggaccggc acacctcgcc ggcatccgcc
      541 ggccgatcgg ccattcggtc ggccctgcac ttgcagtagg ggcgggcatg cgcctggctg
      601 caatgggtga cattcactgc acgcgcgact cgcacggcga gctggcggag acgttcgccg
      661 cagccggcga ggctgccgac ctgctggtgc tgtgcggcga cctgaccgac tacggcctcc
      721 cggccgaggc cgaggtgctc ggccaggagc tggcgagcgc caaggtcccg gtcgtcggcg
      781 tgctgggcaa ccacgaccac gagtccggtg aggccggcga ggtggcccgc atcctggccg
      841 atgccggcgt gcggctgctg gacggtgacg ctgtcgagat cgacggtgtc ggctttgccg
      901 ggaccaaggg cttcggcggc gggttcggcc ggcacgcgct cggctcgtgg ggggagccgg
      961 ccatcaaggc cttcgtgcag gaggcagtgg acgaggcgct gaagctggag aacgccctcg
     1021 cccgcctgcg gaccgacctg cgggtggccg tgctccacta cgcgcccatc cgcgacaccg
     1081 tcgagaacga gccgccggag atcttcccgt ggctggggtc ggagcggctg gaggagccaa
     1141 tcgaccgctt cgagctcacg ctggccctgc acggccacgc ccaccacggc gcgccggagg
     1201 ggcgaacgcg cggaggcgtg ccggtctaca acgtttctgc cccgctgctg cgcgggctgg
     1261 acgagcagcg tccgtttcgc gtcatcgagc ttccagcgca gccggcggcg gcgggcccac
     1321 gcgtggcggc ctccgcgctc gatcgccagt cccaggggct ttgacgtcgt ggggggcagg
     1381 acgcccaccg ccgccaacct cgaccccagg gcggtcgcct tctaccggca tgccatcgag
     1441 gcgctgcggg aggcatcggt cccgttcctg gtcggggggg cctatgccct ggaggcccac
     1501 gccggaatca gcggccggac caaggacctg gacctgttcc tgcgcccgtc cgacgtcgaa
     1561 cgggcgctcc gcgcgctggc ccagtccggc taccgcacgg agctgaccta ccccacctgg
     1621 ctggccaagg cgtacatggg cgacgacttc gtggacctca tcttcagctc cggcaacggg
     1681 ctgggtctgg tggacgacga ctggttcgcc aatccgcgtc gcatcggcgt gctgggctac
     1741 aagctgccgc tggtgcccat cgaggagatc atctggcaga aggcgttcat catggagcgc
     1801 caccgctacg acggggcgga cgtgctgcac ctgatgcgcg ccgcggacgg acacctggac
     1861 gccgatcgcc tgctggaccg cttcgggatg cactggcggg tgctgctggc ccacgtgatc
     1921 ctgttcgact acgtctaccc cgccgagcgc gacctcctgc ctgaccgcct gcgcgccgag
     1981 ctcctgcgga gggccaagga ggagaccgat gcccaggtgg ccgtggccgg caccaacggc
     2041 ggcggcgcca agctgtgctg gggaaccttc ctgtcgtcgt acgagtacgt ggtggacatc
     2101 gagcagttcg ggatggcgga cgctcgccag tcactgcgca aggactagaa gcggcgcacc
     2161 gcgaacacga cgaagaccat caggttgggc accaggccga aggcggcgtc cggccaccac
     2221 aggacgatca gaaacgtgga gagcaccgcg gaggcgacgg ccagccgcag ccaccatgac
     2281 ctgcgcatgc cgaggccgat ggcgctggcc gcgaagccga ggaaggccac cagccacagc
     2341 gcaccaaggg ctacgaggac ctggtcgccg acctcgtagt cgtccggcag cagcgttggc
     2401 tccgctgaga tctgtggcac gaccgcaagt ccagtgctgg ccaggaatcc gagcaggtgg
     2461 atgccgccgt ggatcagcag cagcgccgcc agcgaccagc gccagatcac gatcgttcct
     2521 cccccgctgc ctgggcgatc gcccgtcgcc ccacgcccat cgcagcctcc accggcacgt
     2581 cgatgcgagc gcgctcgacg cgcatctcga accagggcgc gggctcgtcc gcccacaagg
     2641 cgtccacgcg cgatgggtag ggcaggccac ccgccacctg ccacgcgcgc gagccaccga
     2701 accagcggat cttcggtcca cccctggttc gatacctgtc ggcctcgaag cgcaccggaa
     2761 agccggaggt cgggtcgaag tggacggtcg ccacctcggc gtcacgcgag aacggaagcc
     2821 gcgcccgagc agtctgggcg tcgaccgcct cccaggccac tccatcggcc caggcggacg
     2881 gaaggccgat ggcgccggcc cacatcgcca ggaaggcacc ctggtcgacc tcctcaccga
     2941 cggacgcgaa cggcccgatc ttggtgatgc ccctgccgtc cacataggcg tcgaccacgc
     3001 gcagcacggg aaccccgccg gggcgcagcc ggatcaggct gacgtagttg cgccccggct
     3061 cgtggttcat ctgcacctcg atcggcagcc acggcaatgg accgatgcgg acgcgaccgg
     3121 ttccctccat caccaacgtg cgtggggaag gcgcgctccc tccgaagccg acgtcgaggt
     3181 agcgccgcac gggctccggc agcagctcga tgtccatgca cccggcggcg cgcaagactc
     3241 gggccagcga cgcggctgct acgctcgttc caatgcgccg gaccaagctg gtgtgcacca
     3301 tcggccccgc gtcacaggac cgcgtcgacc agctggtggc tgccggaatg gacgtcgcgc
     3361 gcctgaactt ctcccacggg accgatgagg accaccagcg cgccgcggcg gccgtgcgcg
     3421 cggcatccga gcggcagggc cgaccggtgg ccatcctggc cgatctgccg gggccgaaga
     3481 tccgattggg cgacctgccc cacggcgagc tggcgctggt caccggggag cgcttcgcac
     3541 tgggggagga cgcatcggtc acctacgccg ggctggctga cgacatgcgc aacggcgacc
     3601 gactgatgct ggccgatggg gccgtggagc tggaggtgct cggcacgcgc gccgggatcg
     3661 tcgagacgca ggtggtgcgc ggcggcacgg tgcgcagtgg ggcgggcctc aacgggccct
     3721 ccggacggct gcgcctgccc gctgtgaccg accgagatca cgaggcgctt cgcacgatcc
     3781 tggggatggg cgtcgactac gtggcgcagt ccttcgtgcg ccacgcggac gacctgcgcg
     3841 cggtgcgtga gctgatgggc gatcggccaa tgccgatcat ggccaagatc gagacgcgcg
     3901 ccgccgtgga gaacctgggc gccatcctgg aggagaccga tgccctgatg ctggcccgcg
     3961 gcgacctggg cgtggagatc ccgttcgagg acgtgccgct catccagaag cgggccatcg
     4021 acgccagcgt ggccgccggc gtcccggtgg tggtcgccac ccagatgctc gagtcgatgg
     4081 tcagcgcgcc gcgtcccacc cgagccgagg cgaacgacgt cgccaacgcg gtgctcgatg
     4141 gtgcggacgc
//
LOCUS       DATACA010000141         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_180879, whole genome shotgun sequence.
ACCESSION   DATACA010000141 DATACA010000000
VERSION     DATACA010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180879"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VHK28_02885"
     CDS             complement(<1..573)
                     /locus_tag="VHK28_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141211.1"
                     /translation="MLWLLRLAAATVALALACLVPIGSARAAVLWTLVASPLAVSTGV
                     PTTFTLTATNEDPLAALLSEAEIGCVVVDVPANFSVHDATVTGSNSGGSWDAKLTGNR
                     VKVRAGSGGDRLELLGWVRFTIDATALSAGSLTWAAKAYRDQDCEGSTSLLGVPPVVA
                     VSGPAVTPTPQPTVAASPTPQPTTPATPGPT"
     gene            complement(573..>1848)
                     /locus_tag="VHK28_02890"
     CDS             complement(573..>1848)
                     /locus_tag="VHK28_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2141212.1"
                     /translation="AVADAQRDIVIVTLGAAAALAVILFLVFRTAQKRIAHQHAQLLE
                     AERRDPLTGLLNHGAAVTLLADMVERAKVDAAQIGLALVDVDNFRLLNDTHGHQAGDD
                     VLCRVSRLVARGAGANATVARYGPDEFVVVIRDTAAAHLQAMLEGLRIGLGEETIQFG
                     DSENLPISFSAGVAVYPDHADAVTDLLSAATVALAEAKAGGGDATCVARTSDEDERAA
                     TASFDVLQGLIIAVDTKDRYTKRHSEDVARYAVFLANEMGLDEETRRVIHLAGLLHDV
                     GKIGIPDALLRKPGRLSGDEFDVFKQHVALGDAIVREVPHAEQVRAGIRHHHERWDGN
                     GYLAGLEGEEIPLVARILAVADAFSAMTTTRPYRKALPLREALKRLGDAAGDQLEEEL
                     VTTFITGIETASDAPLPGDQPAGIWRPQLWVA"
BASE COUNT          272 a          645 c          656 g          275 t
ORIGIN      
        1 ggttggcccg ggcgttgcgg gggtggtggg ctgtggcgtc ggactcgcgg cgacggtcgg
       61 ctgcggggtg ggcgtcacgg ccgggccgct caccgcgacg accggcggca ctccgagcag
      121 cgaggtgctg ccttcgcagt cctggtcccg gtacgccttg gcagcccatg tcaacgaccc
      181 cgcactcaat gccgtggcat cgatcgtgaa ccgaacccag ccgagcagct ccaggcggtc
      241 gccaccgctg ccggcgcgga ccttgacccg gttgccggtc agcttcgcat cccaggagcc
      301 acccgaattc gagccggtga ccgtcgcgtc gtggacgctg aagttcgcgg gaacgtccac
      361 caccacgcag ccgatctcgg cctcggacag cagggcagcc agcgggtcct cgttggtggc
      421 ggtgagcgta aacgtggttg gcacgccggt gctgacagcg agcggggacg cgaccagggt
      481 ccacagcacg gcagcccgcg ctgaaccgat cggcaccagg cacgccaggg ccagcgccac
      541 ggtcgcggcc gccagcctca ggagccagag catcaggcca cccacagctg cggtcgccag
      601 atgccggccg gctggtcgcc tggcagtggg gcatcggagg ccgtctcgat gccggtgatg
      661 aacgtcgtga ccagctcctc ctcgagctgg tcacccgcag cgtcgccaag gcgcttcagc
      721 gcctcccgaa gcggcagcgc cttgcggtac ggccgcgtgg tggtcatggc gctgaaggcg
      781 tcggccacgg ccaggatgcg ggcaaccaac gggatctcct caccctccag cccggccagg
      841 tagccattcc cgtcccagcg ctcgtggtgg tggcggatcc cggcccgcac ctgctcggcg
      901 tggggaactt cgcgcacgat ggcgtcaccc agggcgacgt gctgcttgaa gacgtcgaat
      961 tcgtccccgc tgaggcgccc gggcttgcgc agcagggcgt ccgggatccc gatcttgccg
     1021 acatcatgca gcagtcccgc caggtggatg acgcggcggg tctcctcgtc caggcccatc
     1081 tcgttggcga ggaagaccgc gtagcgggcg acgtcctcgg agtggcgctt ggtgtagcgg
     1141 tccttggtgt cgaccgcgat gatcagcccc tgcagcacgt cgaagctggc agtggccgcc
     1201 cgctcgtcct cgtcgctggt gcgcgccaca caggttgcat ccccgccgcc ggccttggcc
     1261 tcggcgagcg ccacggtcgc ggccgacagc aggtcggtca ccgcgtcggc gtgatcgggg
     1321 tagacggcca ccccggcgct gaacgaaatg ggcaggtttt cggaatcgcc gaactggatg
     1381 gtttcctcgc cgagcccgat acgcaggccc tcgagcatgg cctgcaggtg ggccgcagca
     1441 gtgtcccgga tgacgaccac gaattcgtcc ggaccatagc gggccacggt ggcgttcgcc
     1501 cccgccccgc gcgccaccag ccgcgacact cggcagagca catcgtcacc ggcctggtga
     1561 ccgtgggtgt cgttcagcag gcggaagttg tcaacgtcca ccaaggccag gccgatctgt
     1621 gcagcgtcga cctttgcccg ctccaccatg tcggccagca gggtcacggc ggcgccgtgg
     1681 ttgagcaggc cggtcaacgg gtcgcggcgc tccgcctcga gcagctgggc gtgctggtgg
     1741 gcaatgcgct tctgcgcggt tcggaagacg aggaacagga tgaccgccag ggccgccgcc
     1801 gctccaaggg tgacgatcac gatgtcccgc tgcgcgtcgg cgaccgcg
//
LOCUS       DATACA010000142         4902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1706551, whole genome shotgun sequence.
ACCESSION   DATACA010000142 DATACA010000000
VERSION     DATACA010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706551"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="VHK28_02895"
     CDS             complement(<1..1859)
                     /locus_tag="VHK28_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX2141213.1"
                     /translation="MAERRPILMLIDGPSLVYRGYFALPPLTTSDGTLTNAAFGFLQI
                     VLRGMQDLKPDYAIVSFDLGKPQFRFDAYPEYKAGRPSMPDDLRSQFPIVRELAQMMG
                     MPIRELEGYEADDVIGSLVQEAKKANVDTYIVSGDLDGLQLVNDHVKLLTTRMGVAAT
                     VIYDEAKVMDRYGLRPDQMLDYKALKGDTSDNIPGVPGVGEKTAIQLLQQFGSLDAIY
                     DNLDQVKGKLRERLAEHRESAFLSREVGRIVTDLPVELKLEEARTGRYDRRAVAQRFR
                     ELEFRSLIDRLPPSSIAPTGFGSPEQETGGGLQLSLDLLGGKAPPADGTGAPHTPRVQ
                     PDAISDPGGAIEHVDPRIVRGDEEMAELESWLDARAEAVGLGWAAGGGRPLERALLGI
                     SLAGTDGSTWYLPWNDDRPLPAWFTRDDRLLIGHDLKQLVTMLAVRGAEVAGPADDTL
                     VASYMVNPSLRAQTLEDLAANRFGATLPERPIIAETGAEEQVAARRAAAEALTALLVR
                     PQLEAELRDGGLLDLYREVEMPLLPVLARVELAGVVIDREALAGMSSEFGAQLAGLEA
                     RIYELVGRPFNIGSPKQLEQVLFHELGLPATKRTRTGFSTDASVLEELREQHEV"
     gene            2091..2597
                     /locus_tag="VHK28_02900"
     CDS             2091..2597
                     /locus_tag="VHK28_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2141214.1"
                     /translation="MADRPVLDPYAVLGVPREATQRQVAHAHRRLAKRFHPDLHPGED
                     VTEPMRRINDAYRLLTSPARRAAFDQAYPNAGARPGGHWAATHRPIKPAAPTSTRTWA
                     DWRTTADEVRAAPRVRRAPGEVQVPLTRRPPRLEPLQGTFLDSGWAAVLVAVVLLTLL
                     AAAIVAGR"
     gene            complement(2594..3286)
                     /locus_tag="VHK28_02905"
     CDS             complement(2594..3286)
                     /locus_tag="VHK28_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141215.1"
                     /translation="MTMNHDVHPHDERLAALASDEPEVAGDAALRAHVSACDRCGPMV
                     AEMTALRSALAELPDLVPSRPLQLLPPVPEPAASGAGIGLLRRLWGPFMVAGAGLALV
                     GAIGLSGGTEGLMQGMSAEDAGAPGFETMSSDPRMERATDIPAAPSGAVQFNPDAAGS
                     PAAEGATGNDEGEVTGDNLAGGGESGEGDGRADLLATDQGDAAWWAVVFAGIVLILVA
                     LALRFRVQPRAG"
     gene            complement(3301..3975)
                     /locus_tag="VHK28_02910"
     CDS             complement(3301..3975)
                     /locus_tag="VHK28_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2141216.1"
                     /translation="MPPTLPSPQEPASDPSAPTSEALTPPRPAGRTRTAPLPDEAEVV
                     ARAAAGDRGAFTRLMEHYQSACYGLAYRLLGDADQAADATQDAFVHAYDALGKYRGGV
                     FRSWLLRITANASYDILRRAQRRPTTTLPDADEGEAELPDVTAPNPVAEAGRSELYRH
                     LDAALRRLPEDQRVAVVLCDVYGMDYNEVASFTQSALGTVKSRIHRGRLRLRELLADH
                     RELFEG"
     gene            4008..4094
                     /locus_tag="VHK28_02915"
     tRNA            4008..4094
                     /locus_tag="VHK28_02915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4042..4044,aa:Leu,seq:caa)
     gene            complement(4128..4793)
                     /locus_tag="VHK28_02920"
     CDS             complement(4128..4793)
                     /locus_tag="VHK28_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141217.1"
                     /translation="MSEKLRVLLEIGPKGKRIVAAAMDWPGLERWGRTEPEAVDKLLA
                     YVPRYVPVAEQVGLRSELERGVEPHIVERTPGNTSTDWWGVAHVPAVVEAEPLGDDEL
                     ERRIGLMQAAWDYFNHASESGVAPLTKGPRGGGRERDQIVRHVYASERHNWWRKVGIR
                     EDDELRLTPKQLAALRERYVAAIRQYHAEGRPARRWPLRFLIRRTAQHAMDHAWELED
                     RTP"
BASE COUNT          704 a         1755 c         1691 g          752 t
ORIGIN      
        1 acctcgtgct gctctcgcag ctcctccagg accgatgcgt ccgtcgagaa cccggtccgc
       61 gtgcgcttcg tcgccggcag gcccagctca tggaagagga cctgctccag ctgcttggga
      121 gaaccgatgt tgaacggtcg ccccacgagt tcgtagatcc gcgcctccag cccggccagc
      181 tgggcgccga attcggagct catgccggcc agcgcctcgc gatcgatcac cacaccggca
      241 agctcgacgc gtgccagcac cgggaggagc ggcatctcca cctcgcggta gaggtcgagc
      301 aggccgccgt cccgcagctc ggcctccagc tgggggcgga ccaggagggc ggtcagcgcc
      361 tcggccgcag cccggcgcgc cgcgacctgc tcctccgcgc cggtctcggc gatgatcggc
      421 cgctccggga gcgtggcccc gaagcgattg gcggccaggt cctccagggt ctgcgcccgc
      481 agcgaggggt tgaccatgta gctggccacc agggtgtcgt ccgcgggacc ggcgacctcg
      541 gcgccacgca cagccagcat ggtcaccagc tgcttgaggt cgtggccgat cagcaggcgg
      601 tcgtcgcggg tgaaccaggc cggcaacggc cggtcgtcgt tccagggcag gtaccaggtg
      661 ctcccgtcgg taccggccag gctgatgccc agcagcgccc gctccagcgg ccttccgccg
      721 cctgcagccc agcccaggcc gaccgcctcc gcgcgggcgt cgagccagga ctccagctcg
      781 gccatctcct cgtctccacg cacgatccgc gggtcgacgt gctcgatggc gcccccgggg
      841 tcgctgatgg cgtccggctg gacgcgaggg gtgtgcggcg cgccggtgcc gtcggccggc
      901 ggcgccttgc cgcccagcag gtccagggag agctgcaggc ctccgccggt ctcctgttcg
      961 ggcgaaccga aaccggtggg tgcgatgctg gatggcggca ggcggtcgat gagcgaccgg
     1021 aactcgagct cccggaagcg ctgcgcgacc gctcggcggt cgtaccggcc ggtgcgtgcc
     1081 tcctcgagct tcagctcgac cggcaggtcg gtcacgatcc gcccgacctc gcgcgacagg
     1141 aacgccgact cgcggtgctc cgccagccgc tcacggagct ttcctttgac ctggtccagg
     1201 ttgtcataga tggcgtccag cgacccgaac tgctggagga gctggattgc ggtcttctcc
     1261 cccaccccgg ggacgccggg aatgttgtcc gaggtgtcgc ccttgagggc cttgtagtcc
     1321 agcatctggt ccggccgcag tccgtatcgg tccatgacct tggcttcgtc gtagatcacg
     1381 gtagcggcta cgcccatccg ggtggtcagc agcttcacgt ggtcgttgac cagctgcagg
     1441 ccatccaggt caccagacac gatgtaggtg tcgacgttcg ccttcttggc ctcctggacc
     1501 aggctgccga tcacgtcgtc ggcctcgtag ccctccagct cgcggatcgg catgcccatc
     1561 atctgcgcca gctcgcgcac gatcgggaat tgcgaccgca ggtcgtccgg catggacggg
     1621 cgcccggcct tgtactccgg gtaggcatcg aagcggaact gaggcttgcc gaggtcgaag
     1681 ctgacgatgg cgtagtccgg cttgaggtcc tgcattccgc gcaacacgat ctgcaggaag
     1741 ccgaacgccg cattggtcag ggtgccgtcc gaggtcgtca gcggcgggag cgcgaagtaa
     1801 ccgcggtaga cgaggctggg cccgtcgatg agcatgagga tcggtctgcg ctcggccatc
     1861 ggagggccaa gtctagaccg atgtcttgcc gcggggatct gtcgatgctg acgcctcgaa
     1921 caggtcggat tcacaccagg tgcactgacc gcgagaaacg gccgttgaag gcacctgatt
     1981 cacgccagct gtgaattcgg acgtctcagg caccgttccg aggccggact ggtcacctca
     2041 cgtgaattcg gacgctctcg cgcgcggcac ccggaaccta caatcggagg atggcggacc
     2101 gcccggtgct cgacccgtac gccgtcctcg gcgtgccgcg cgaagcgaca caaaggcagg
     2161 tggcgcacgc ccatcggcgc ctggccaagc ggttccaccc ggacctccac cccggcgagg
     2221 acgtgaccga accgatgcgc cgcatcaacg acgcgtaccg cctgctgacc tctccggcac
     2281 ggcgcgccgc gttcgatcag gcctatccga atgccggcgc gcggcccggc gggcactggg
     2341 cagcgacgca tcggcccatc aagccggcgg cgcccacgag cacacgaacc tgggcggact
     2401 ggcggacgac cgccgatgag gtgcgtgccg ccccgcgcgt ccgacgcgcg ccgggcgagg
     2461 tccaggttcc gctgacccgc cggccgccac ggctggaacc gctgcagggg accttcctgg
     2521 acagcggctg ggcggcggtt ctggtcgcgg tggtgctgct gacgttgctg gccgcggcga
     2581 tcgtggccgg tcgctagccg gcgcggggct ggacccggaa gcggagggcg agcgccacca
     2641 aaatcaggac gatgccggcg aagacgacgg cccaccaggc cgcatctccc tggtcggtgg
     2701 cgagcaggtc ggcccgacca tccccctccc cggattctcc tccaccagcc aggttatccc
     2761 cggtcacctc accttcgtcg ttgcccgtgg ccccctcggc cgccggcgag cctgcagcat
     2821 ccgggttgaa ctgcaccgca cccgaaggtg cggccgggat gtccgtcgcg cgctccattc
     2881 gcgggtcgct gctcatcgtc tcgaagccgg gtgcgccggc gtcctccgcg gacatgccct
     2941 gcatcaggcc ttcggtccct ccgctgagac cgatggcgcc caccagcgcc agcccggcac
     3001 ccgcgaccat gaacggtccc cacagccgcc gcagcagccc gatgccggcc ccgctggctg
     3061 ccggctcggg aaccggtggc aggagctgca atggccgcga gggcacgagg tccggcagct
     3121 cggccagcgc cgagcgcagg gcggtcatct cggcgaccat cgggccgcat cgatcgcagg
     3181 cggagacgtg cgcgcgcagc gccgcgtcgc cagcgacctc gggctcgtcg ctcgccagcg
     3241 ccgccaggcg ctcgtcatga gggtgaacgt cgtgattcat cgtcatcggt ccagacggca
     3301 tcagccttcg aagagttccc ggtggtcggc gagcagctcg cgaagccgca gccgccctcg
     3361 gtggatccgg gatttcacgg tgcccagcgc cgactgcgtg aagctggcca cctcgttgta
     3421 gtccatgccg tagacgtcgc acagcacgac cgccacccgc tggtcctcgg gcaggcggcg
     3481 cagggcggcg tccaggtggc ggtacagctc gctgcggcca gcctccgcga ccgggttggg
     3541 agccgtcacg tccggcagct cggcctcgcc ttcgtccgcg tccggcaggg tggtcgtcgg
     3601 gcggcgctgc gcccgacgca ggatgtcgta gctggcgttg gccgtgatgc gcagcagcca
     3661 ggagcgaaag acgccgccgc ggtacttgcc caatgcgtcg taggcatgga cgaacgcgtc
     3721 ctgggtggca tccgccgcct ggtccgcgtc gccgaggaga cggtaggcca gtccgtagca
     3781 ggccgactgg tagtgctcca tcagccgggt aaaggcgcca cgatcgcctg ccgcggcgcg
     3841 ggcgaccacc tccgcctcgt ccggcaaggg cgccgtccgc gtccggccgg caggtcgcgg
     3901 cggggtgagc gcctcggagg tcggggcgct cgggtcgctg gctggctcct gggggctcgg
     3961 cagggtagga ggcatggcct ctctataatc acatcactcc ccccatcgcc ggagtggcgg
     4021 aatcggcaga cgcgtcggtc tcaaacaccg atgcccgcaa gggcgtgtgg gttcgaatcc
     4081 cacctccggc accaccctga ttcgtaacac cgtagccgcg gtggcactca gggtgtgcgg
     4141 tcttccagct cccacgcgtg gtccattgcg tgctgcgccg tgcggcggat gaggaagcgc
     4201 agcggccagc ggcgcgctgg ccgcccctcg gcgtggtact gccggattgc cgccacgtag
     4261 cgctcgcgca gcgcggcgag ctgcttcggg gtcaggcgca gctcgtcgtc ctcacgaatg
     4321 cccaccttgc gccaccagtt gtgccgttcc gacgcataga catgacggac gatctggtcc
     4381 cgctcgcgtc ccccaccccg cggccccttg gtcagaggcg ctacgcccga ctctgacgcg
     4441 tggttgaagt agtcccacgc cgcctgcatg agcccgatcc gccgctcgag ctcgtcatcg
     4501 ccgagtggct cggcctcgac gacggctggc acgtgggcca caccccacca gtccgttgat
     4561 gtgttgcccg gcgtgcgctc cacgatgtgc ggctcaacgc ctcgctccag ctcggagcgg
     4621 aggcccactt gctccgccac cgggacgtag cgtggcacgt aggcgaggag cttgtcgacc
     4681 gcctcgggct cggtacgccc ccagcgctcc aggcctggcc agtccatggc ggcggccacg
     4741 atccgcttgc ccttcggacc gatctcgagg aggacacgca gcttttctga catgccaaca
     4801 ggctacggca gcttgggtat gtggggccgg cgacggtacc tccgtactct ggcgatcgca
     4861 ccagcagcga gttagcgtct cgatcgggcc ggtccgggca tt
//
LOCUS       DATACA010000143        10524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_181406, whole genome shotgun sequence.
ACCESSION   DATACA010000143 DATACA010000000
VERSION     DATACA010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181406"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /gene="cpsB"
                     /locus_tag="VHK28_02925"
     CDS             <1..291
                     /gene="cpsB"
                     /locus_tag="VHK28_02925"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="capsular polysaccharide colanic acid biosynthesis
                     protein; catalyzes the formation of GDP-mannose from GTP
                     and alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX2141218.1"
                     /translation="VVPLDAGWSDIGSWSALLEALSTQSGHSLVASGRHLDRGSSNVL
                     VQGGERLIVTVGLEDVIIVDTPDALLVCSRDRAEEIKPVLDEIAEKAGERYL"
     gene            311..1051
                     /locus_tag="VHK28_02930"
     CDS             311..1051
                     /locus_tag="VHK28_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141219.1"
                     /translation="MPPTSVRPGPPARRRPPVIEDRVRFAWAGRLIGRAMALYVRLVA
                     ATARTSGPEVAQEQLIWAIWHESNLATAVAALALRGDTQAIVFTTRGFRGIVMNAMLH
                     GIGSGAVTVPPEGAAGRAEAASVSRAMSRLTREGWSVLASCDGPFGPRRVVKPGVLIV
                     ARESGLPVQPWALAIRPPLRLRRRWDQQLVPLPLCRLTVVEGRQLRLEPRERIKPRLV
                     ELQAELDRITALAERRMAGRRTAARGRS"
     gene            1126..2580
                     /locus_tag="VHK28_02935"
     CDS             1126..2580
                     /locus_tag="VHK28_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEX2141220.1"
                     /translation="MTRIKFGTDGWRAAIAEEYTFHNVRRCARAVAEYLQDTGAADRG
                     VVVCHDRRFASEHFARACVEVLAAHDIRSYAPPEAVPTQVGSYFTRELGAGAGIVITA
                     SHNPWTDNGFKVKADTGGAAAPEMLTAIEATMDRHPDEEVPPRRDMADARAAGLVEEF
                     DPYPRFREQLERIVDLDKIKAADRRVLVEPLYGSGAGWFTRLLGDGRMTIRELHTERN
                     PYFGGVNPEPIRPNIDAWLEEIPRLGADIGIAFDGDADRVGMATEEGVFVNQLQVYGL
                     LYWYLLEVRGQIGPAVYTVTTTSMAPRLAEIFGTQAYETGVGFKYVGPKMTETDAVIG
                     GEESGGFGFGMHIPERDGLASGLFLLDLWLTKGRRASEVLAELQALAGPSYYNRIDIR
                     FDRDGYESVKADTLARLEHEAPESLAGRAVDRRIPLETRDGFKFYRDDGSWLLIRFSG
                     TEALLRIYTEARSPADVEALLAEGRTIATAGVAA"
     gene            2594..3769
                     /locus_tag="VHK28_02940"
     CDS             2594..3769
                     /locus_tag="VHK28_02940"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX2141221.1"
                     /translation="MSDARDFFGYRKPDDEPGRPDPEDGDQPGDSPPQFDEAITLDSP
                     EVLRRIDTSDMLGRVRELPRQVSMGRRVAAAIELEPRHRDVDAVLVLGMGGSAIGAEL
                     VAGVAGDRLRVPLVIHRDYGLPGWANQRTLVIAASHSGETVETLSAADEARRRRLPLV
                     VASTGGRLGRDASGDGTPYLHYQAGGQPRAAVGFGMVLVHELLASAGLLADPDPYGPA
                     VEALESVLERHGPAVETDANPAKQLAWGIFGRIPVIYGAGAMTAVARRWKTQLNENTK
                     SWASYEPMPEANHNAIEGSLNPRELSDALYVVQVREREEAPAIAERYQVVEELLGERA
                     TNRSIVWAEGPSPLARVLTAVAIGDLVSVYLAILYQTDPTPVTLLAMLKERLARSTD"
     gene            3832..4101
                     /locus_tag="VHK28_02945"
     CDS             3832..4101
                     /locus_tag="VHK28_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141222.1"
                     /translation="MIGKVEKAMRYAHEPDRVKIQSFTASFAGDNGSHLVSLDGDTWQ
                     CDCHLFEAAGGCTHTLAIQKMLDPMLTEMAKETPLYGRAELAAPV"
     regulatory      4212..4355
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4382..5380
                     /locus_tag="VHK28_02950"
     CDS             4382..5380
                     /locus_tag="VHK28_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2141223.1"
                     /translation="MAPRLRASSVLALALVLSACAPGAATVPPSAADSASVQASASGT
                     ASPAAAYPLTLTDDAGRSVTLDGDPQRIVSLAPSNTEIVCALGSCEQIVGVNDFRDGL
                     PDDVLATLEDIPDVATFTGVDREALVAAEPDLVLAAGNELTSSADIQAIDELGIPVLV
                     LYPESLEEVYADIELVGAALDEQASAEELVSSMEIEVAEIEAEVRGAERPRTFYEVGL
                     FEGSIYTAGEDSFLASLIELAGGDPIVGDAVTTTISTEELVAADPQVILLGDAAYDPS
                     ITPEAVAARPGWEGMSAMQDGRVVPMPEDVLITRPGPRITQGLEALARAIHPEAFD"
     gene            5380..6495
                     /locus_tag="VHK28_02955"
     CDS             5380..6495
                     /locus_tag="VHK28_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX2141224.1"
                     /translation="MRATAATPAALGRLPSGPTLTRAALVLGGGVLVLLVVMVAAVAA
                     GSVGVGPADTAAILARRLLGLPIGETWTPASETIIFELRLPRVLTAMLVGGGLAMAGT
                     VFQAVLRNPLADPYIIGTAAGASLGAVAGIVLPLLLPALALGAGTSWLGLGLVQVLAF
                     AGGLGTVLVVYGVARSGGRVPVVTLLLTGYAVSSVLAATVALMMFLSGRALAAVFSWL
                     MGSLADASWSELAFSVPLLAASLALLVVRWRGLNLLTLGDSAAANLGLDVEREKLVLT
                     MLATLATSAAVAISGTIGFVGLVVPHLLRLAIGPDHRLLLPASVLFGAALLALADLGA
                     RLIGGVPVGVVTALVGAPFFLWLLRRSRAAGAGTVNA"
     gene            6492..7337
                     /locus_tag="VHK28_02960"
     CDS             6492..7337
                     /locus_tag="VHK28_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141225.1"
                     /translation="MSDGGSQPLVQLDGVTYGYPLPGGRRRPVLTAVDLAFGRGELLA
                     VLGPNGSGKTTLLRLITGVLRSQSGSVSFDGVPVAEWSRSRLARRVAVLPQSLELPAG
                     FRVAELVEMGRAPHARRLFGSTADDERAVLRALAEADALDLAHRTADQLSGGERQRVL
                     VAMALAQEPELLLLDEPTLHLDLAHQVSLLNTIARLREQRGLTVVAVLHDVNLASALA
                     PRVVVLHDGGVVADGPPEAVLEPPLVTRAFGVSVTEAWTAEGQRHLALADVTASARTP
                     QRPRP"
     gene            complement(7382..8638)
                     /locus_tag="VHK28_02965"
     CDS             complement(7382..8638)
                     /locus_tag="VHK28_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2141226.1"
                     /translation="MADPGPLAASGPRERRWLLVFLILGSAYFAILVAQEALGFLGGF
                     SQIILIVFLAWLLAFVMSPVVRFLDEGLPLPRPLAVAIAYGLALLTLGFVLFYVGSSI
                     TQEVTLVTVEFPGTARQIEKQLLETQQAFGLDRFQVDLVELFQSAQRQVGNVAGAIFE
                     QAEEIAGATLATLGSLVLILILSLYMVLDRDRIVAKINRAVPRRYSDEMQILERSVAR
                     AFGGFLRAQVILAAIQALLVTVIGIAFDMPYLFLVGTLSTLAMLIPFFGPPLALIPPI
                     VAAWIYVPDGFIPITILLVGIQTVIVNWLQPRLMQDSLGIHPILVLVGLLVGAQVAGV
                     WGALFGIPVIAVLNVFLNLVVWSELPNAALPAEERLEDVPEATMVKVEREQVSDETHP
                     HIHVHRSLRPDGTEQVDLVMDEEEGI"
     gene            complement(8644..9705)
                     /gene="trpS"
                     /locus_tag="VHK28_02970"
     CDS             complement(8644..9705)
                     /gene="trpS"
                     /locus_tag="VHK28_02970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2141227.1"
                     /translation="MPDIMGLHSLPMSQAAPAPTTTAPATRERVFSGIQPSGASHLGN
                     FLGAQANYVAMQDEYDAIYAIVDLHALTTVHDGAKLRQLTHDMVLDLLAVGLDPDRCA
                     IVRQSDLVEHAELQWIFNTVVPVTWLERTPTYKEKRAEGIENSNGLLSYPVLQAADIV
                     IYKATRVPVGKDQAAHLELSREIVRAFNRRYGEIFPEPQAVFTDAPTVLGLDGARKMS
                     KSANNTIPIFAEPDEIRRLVMSMVTDPQRIKRSDPGRPEICNVCQLHRFFGGDYLQIQ
                     DGERTARTGCVDTKQLLAERIVEFFRPMRQRRDEMAADPGHVEAVLAAGAAKVRPILE
                     ETMREVRAAVGIGPDGRRA"
     gene            9704..10501
                     /locus_tag="VHK28_02975"
     CDS             9704..10501
                     /locus_tag="VHK28_02975"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX2141228.1"
                     /translation="MTDASAWQRAADPARRIGHVVEFHPSIGSTSDRARELLAAGQEG
                     VAVVADEQTAGRGRRGRSWASPPGVNLMVSVAFRPNLEPEGLGHLGLGAALAVRAACM
                     TVAPTTRIWLRWPNDLVADDGGKLAGVLVETSIAEGQPVDAVVGMGINVNWARGEMPA
                     EIGARATSLGELGGGEVDRVALLAALLRGVDGELSALERGLSPVPRARKASALDGRWV
                     QVETGNGALSGTVVGIDDDGALLLEADGERHALHVGEVVAVRDVVPA"
BASE COUNT         1504 a         3635 c         3829 g         1556 t
ORIGIN      
        1 gtcgtgccgc tcgacgcggg ttggagcgac atcggctcct ggtcggcgct gctggaggcg
       61 ctgtccacgc aaagcgggca cagcctggtg gcctcggggc gtcacctcga ccgcgggtcg
      121 agcaacgtcc tcgtccaggg cggggagcgg ttgatcgtga ccgtcggcct ggaggacgtc
      181 atcatcgtgg acacgccgga tgccctcctg gtctgctccc gggaccgcgc cgaggagatc
      241 aagccggtgc tggacgagat cgccgagaag gccggcgagc gctatctctg atccatcggc
      301 tctcggaccg atgcctccga cgtccgtacg tcccggtccg cccgcccggc gaaggcctcc
      361 cgtcatcgag gaccgcgtca ggttcgcctg ggccggccgc ctcatcggtc gcgccatggc
      421 cctctacgtc aggctggtgg ccgccaccgc ccgaacgtcg gggccggagg tcgcccagga
      481 gcagctgatc tgggccatct ggcacgagtc caacctggcc accgcggtcg cggcgctggc
      541 cttgcgcggg gacacccagg cgatcgtctt caccacccgc ggcttccggg gaatcgtgat
      601 gaacgccatg ctgcacggca tcggctcggg agcggtcacc gtcccgccgg agggagccgc
      661 cggtcgcgcc gaggccgcca gcgtgtcgcg tgccatgtcc cgactgacgc gcgagggctg
      721 gagcgtgctg gccagctgcg acggcccgtt cgggccgcgg cgggtggtga agcccggcgt
      781 cctcatcgtg gcgcgagagt ccgggctgcc agtgcagccc tgggcgctgg ccattcggcc
      841 accgctgcgc ctgaggcgcc gctgggacca gcagctggtg ccgctgccct tatgccggct
      901 cacggtggtc gagggccgtc agctgcggct cgagcctcga gagcggatca agccgcgcct
      961 ggtcgagctc caggccgagc tggaccgcat cactgcgctt gccgagcgac gaatggcagg
     1021 ccggcgcacg gctgctcgcg gccgctccta gcgcgatccg gatggcagtt ggcgcgacgc
     1081 tcgctaccat agtcggcatc tgacagtgga gtgccctccc gccccgtgac gagaatcaag
     1141 ttcggaaccg acggctggcg cgccgccatc gccgaggagt acacgttcca taacgttcgc
     1201 cgctgcgccc gggcggtggc ggagtacctc caggacaccg gtgcggcgga ccgcggcgtc
     1261 gtcgtctgcc acgaccggcg cttcgcctcc gagcacttcg cgcgcgcctg cgtggaggtg
     1321 ctggcggcgc acgacatccg ctcctacgca ccgcccgagg cggttccgac ccaggtcggc
     1381 agctacttca cccgggagct gggtgccggc gccggcatcg tgatcaccgc cagccacaat
     1441 ccctggaccg acaacggctt caaggtgaag gccgataccg gcggcgccgc cgcgccggag
     1501 atgctgaccg ccatcgaggc caccatggat cgccatccgg acgaggaggt gccgccgcgc
     1561 cgcgacatgg ccgacgcgcg tgccgccggg ctggtcgagg agttcgaccc atacccgcgc
     1621 ttccgcgagc agctggagcg gatcgtggac ctcgacaaga tcaaggccgc ggaccggcgg
     1681 gtgctggtcg agccgctgta cggctcgggc gccggctggt tcacccgcct gctcggcgac
     1741 gggcggatga ccattcgcga gctgcacacc gagcgcaacc cgtacttcgg cggtgtcaac
     1801 ccggaaccga tccggcccaa catcgacgcc tggctggagg agatcccgcg cttgggcgcg
     1861 gacatcggga tcgccttcga cggcgacgcg gaccgggtcg gcatggccac cgaggagggt
     1921 gtcttcgtca atcagctgca ggtctacggg ctgctctact ggtacctgct cgaggtgcgc
     1981 gggcagatcg gtccggcggt ctacacagtc accaccacct ccatggcgcc gagactggcc
     2041 gagatcttcg gcacgcaggc ctacgagacc ggtgtcggct tcaagtacgt cggtcccaag
     2101 atgaccgaga ccgatgcggt catcggcggc gaggagtccg gcggcttcgg attcggcatg
     2161 cacattcccg agcgcgacgg attggcatcc ggcctcttcc tgctggacct gtggctgacc
     2221 aagggccgca gggcgtccga ggtgctggct gagctgcagg ccctggccgg tccgtcgtac
     2281 tacaaccgga tcgacatccg cttcgaccgc gacggctacg aatccgtcaa ggccgatacg
     2341 ctggcgcggc tggagcacga ggcgccggag agcctggccg gccgggccgt ggatcgccgc
     2401 atcccgctcg agacgcgcga cgggttcaag ttctaccgcg atgacggctc gtggctgctc
     2461 atccgcttca gtggcaccga ggcgctgctg cgcatctaca ccgaggcgcg ctccccggcc
     2521 gacgtcgaag cgctgctggc cgagggccgc acgatcgcca ccgcgggggt ggccgcgtga
     2581 gccagcgcac ggcatgagcg acgcccgcga cttcttcggc taccgcaagc cggacgacga
     2641 gccgggccga ccggacccgg aggacggcga ccagcccgga gactcgccgc cgcagttcga
     2701 cgaggcgatc accctagaca gccccgaggt gctgcgccgc atcgacacct cggacatgct
     2761 cggtcgggtg cgcgagctgc cgcgccaggt gtcgatgggc cgtcgggtgg cggccgccat
     2821 cgagctcgag ccccggcacc gtgacgtgga tgccgtgctg gtgctgggca tgggaggttc
     2881 ggccatcggc gccgagctgg tggccggggt ggccggcgac cggctccgcg tgccgctggt
     2941 gatccatcga gattacgggc tgccggggtg ggccaaccag cggacgctgg tgatcgcggc
     3001 cagccactcc ggtgagacgg tggagacgct gtcggccgcc gatgaggcgc gccgccgccg
     3061 cctcccgctt gtcgtggcca gcaccggtgg gcggctcggc cgcgacgcca gcggcgacgg
     3121 cacgccgtac cttcactacc aggccggcgg gcagccgcgc gccgccgtcg gattcggcat
     3181 ggtgctggtg cacgagctcc ttgcttcggc cggcctgctg gccgaccccg acccgtacgg
     3241 gccggcggtg gaggcgctcg aatcggtcct ggagcggcac ggcccggcgg tcgagaccga
     3301 tgccaacccg gccaagcagc tggcgtgggg gatcttcgga cgaatcccgg tgatctacgg
     3361 cgcgggcgcc atgacggcgg tcgcgcgacg ctggaagacg cagctcaacg agaacaccaa
     3421 gtcgtgggca agctacgagc cgatgcctga ggccaaccac aacgccatcg agggcagcct
     3481 caacccgcgc gagctctcgg acgcgctgta cgtggtccag gtgcgcgagc gggaggaggc
     3541 gccggccatc gccgagcgct accaggtggt cgaggagctg ctcggggagc gggcgaccaa
     3601 ccgctcgatc gtgtgggccg agggcccgtc gcccctggcc cgggtgctga ccgcggtggc
     3661 catcggcgac ctggtgagcg tgtacctcgc gattctctac cagaccgacc cgaccccggt
     3721 tacactgttg gcaatgttga aggagcggct cgccaggtcg acggactgac gggcgccgat
     3781 agatcggcgc cggatgccgg cgccgggagg aaacgcaccg tgcattcgag catgatcggc
     3841 aaggtcgaaa aggcaatgcg ctacgcgcac gagccagatc gggtgaagat ccagtccttc
     3901 accgccagct tcgccggcga taacgggagc cacctcgtct cgctcgacgg cgacacctgg
     3961 cagtgcgact gccacctctt cgaggctgca ggcggctgca cccataccct tgccatccag
     4021 aagatgctcg atccgatgct gaccgagatg gccaaggaga cgccgctcta cggccgcgcg
     4081 gagctcgccg cgccggtgta ggtccgcggc tgcgtccgca ttcgaggccc gcctcggccc
     4141 ctcggccggc gggcctttct catgcccgcg ccgccggccg cgcgccgacc gctctgctac
     4201 gatccgctga ccaactgaag accgcgaagg gtgccgccgc cgaaaggccg gcgggtaaat
     4261 ggcgaagccg gtgcgaagcc ggcgctgtcc cgcaactgta tccgcgccgc gaggcgtggg
     4321 agccaggtcg cccagcccca tcgcgatgtc aagaccctcg agtgaaaggg aacggcatca
     4381 catggctcct cgtctccgcg cctcctccgt actggcactt gccctggtcc tgtcggcctg
     4441 cgcgccgggt gccgccacgg tgccaccatc ggcagctgat tcggcatccg tccaggcatc
     4501 ggcttcgggt acggcgtctc ccgccgctgc atacccactc accctgaccg atgacgccgg
     4561 gcgcagcgtc acgctcgacg gcgatccgca gcggatcgtc agcctggcgc cgtcgaacac
     4621 ggagatcgtg tgcgcgctgg gctcctgcga gcagatcgtg ggcgtcaacg acttccgcga
     4681 cggcctgcca gatgacgtcc tggccaccct cgaggacatc ccggacgtgg ccaccttcac
     4741 cggtgtggac cgcgaggccc tggtcgccgc cgagccggac ctggtgcttg cggcgggcaa
     4801 cgagctgacc tccagcgccg acatccaggc catcgacgag ctcggtattc cagtgctggt
     4861 cctctacccc gagtcgctcg aggaggtgta cgccgacatc gagctggtcg gcgccgcgct
     4921 ggacgagcag gcgagcgcgg aggagctggt cagcagcatg gagatcgagg tcgccgaaat
     4981 cgaggcggag gtgcgcggcg ccgagcggcc gcggaccttc tacgaggtcg ggctcttcga
     5041 gggctcgatc tacaccgctg gcgaggactc gttcctggcc tcgttgatcg agctggcagg
     5101 cggcgacccg atcgtcggcg acgcggtcac caccaccatc tccaccgagg agctggtggc
     5161 agccgacccg caggtcatcc tgctgggcga cgccgcctat gaccccagca tcaccccgga
     5221 ggccgtggcc gcccgacccg gttgggaggg catgagcgct atgcaggacg gccgggtggt
     5281 cccaatgccg gaggacgtgc tcatcacgcg gcccggaccg cgtatcaccc aggggctgga
     5341 ggcgttggcg cgcgccatcc acccggaggc gttcgactga tgcgcgccac ggccgccacg
     5401 cccgccgcgc tcggccgcct gccgagcggg ccaacgctga cgcgcgcggc gctggtgctg
     5461 ggcggtggcg tgctggtgct gctggtggtg atggtggcgg ccgtggccgc cggcagcgtc
     5521 ggagtgggac cggccgatac ggcggccatc ctcgcccgca ggctgctcgg cctgccaatc
     5581 ggcgagacct ggacgccggc cagcgagacg atcatcttcg agctgcgcct gccgcgggtc
     5641 ctgaccgcca tgctggtcgg cggcggcctg gcgatggccg gcaccgtctt ccaggccgtg
     5701 ctgcgcaacc cgctggccga cccgtacatc atcggcaccg cggcgggcgc cagcctggga
     5761 gcagtggccg gcatcgtcct gccgctgctg ctgccggccc tggcgctggg ggccggcacc
     5821 agctggctgg ggctgggcct ggtgcaggtc ctggcgttcg ccgggggttt ggggacggtg
     5881 ctggtcgtct acggcgtggc ccgcagcggc ggacgggtac cggtggtgac cctgttgctg
     5941 accgggtacg cagtgtcatc ggtcctggcc gcgacggtgg cgctcatgat gttcctctcc
     6001 gggcgcgcgc tggcagccgt gttcagctgg ctcatgggct cgctcgccga cgcctcgtgg
     6061 tcagagctgg ccttctcggt gccgctgctg gccgccagcc tggccctgct ggtcgtccgc
     6121 tggcgcggcc tgaacctgct gaccctcggc gactcggccg cggccaacct tggcctggac
     6181 gtcgagcgcg agaagctggt gctgaccatg ctcgccacgc tggcaacctc ggcggcggtg
     6241 gccatctctg gcaccatcgg attcgtgggc ctggtggtgc cgcacctgct gcggctggcg
     6301 atcgggccgg atcaccgact gctgctgccg gccagcgtac tgttcggtgc cgcgctgctg
     6361 gcactggcgg accttggcgc gcggctgatc ggcggggtgc cggtgggcgt ggtgaccgcc
     6421 ctggtcggag cgcccttctt cctgtggctg ctgcgccgat cgcgggctgc cggggccggg
     6481 acggtaaacg catgagcgac ggaggttctc agcctctcgt ccagctcgac ggcgtcacct
     6541 acggctatcc cctgcccggt ggacggcgcc gtccggtgct gaccgccgtg gacctggcct
     6601 tcgggcgcgg cgagctgctg gcggtgctgg ggcccaatgg cagcggcaag accaccctgc
     6661 tgcgcctgat caccggcgtg cttcggtcac agtccgggtc cgtctcgttc gacggcgtgc
     6721 cggtcgcgga gtggtcgcgc tcccgcctcg ctcggcgggt ggcggtgctg ccgcagagcc
     6781 tggagctgcc ggccgggttt cgggtcgccg agctggtgga gatgggccgc gccccgcatg
     6841 cccgccgcct gttcggttcg acggccgatg acgagcgggc cgtgctccgc gcgctagccg
     6901 aggcggacgc gctggacctc gcccaccgaa cggcggacca gctctccggc ggcgagcgcc
     6961 agcgcgtcct ggtggccatg gcgctggccc aggagccgga gctgctgctg ctcgacgagc
     7021 ccaccctgca cctggacctg gcgcaccagg tgtcgctgct gaacacgatc gcgcggctgc
     7081 gggagcagcg tggcctgacg gtggtggcgg tgctgcacga cgtcaacctt gcgtccgccc
     7141 tggcgccgcg ggttgtcgtg ctgcacgacg gcggcgtggt ggcggacggg cccccggagg
     7201 cggtgctgga gcctcccctg gtgaccaggg ccttcggcgt gtccgtcacc gaggcgtgga
     7261 cggcggaggg ccagcggcac ctggccctcg ccgatgtgac cgcttcggcg cgaacgccgc
     7321 agcgaccacg cccctaggct agccgagaat cgcggatgac gccgaggaag cccggttcgg
     7381 gctagatccc ctcctcctcg tccatcacca ggtcgacctg ctcggtgccg tccggacgca
     7441 gcgagcggtg gacatggatg tgcgggtggg tctcgtcgct gacctgttcg cgctcgacct
     7501 tgaccatggt tgcttccgga acgtcctcca gccgctcctc ggcggggagg gcggcgttcg
     7561 gcagctcgga ccagaccacc aggttgagga agacgttgag cacggcgatg accgggatgc
     7621 cgaacagggc gccccacacg ccggccacct gcgctccgac cagcaggccc accagcacca
     7681 ggatggggtg gatgcccagc gagtcctgca tgagccgggg ttgaagccag ttgacgatca
     7741 ccgtctggat gccgaccagc aggatagtga tcgggatgaa gccgtccggc acgtagatcc
     7801 aggccgccac gatgggcggg atgagggcca gcggcggccc gaagaagggg atcagcatgg
     7861 ccagggtgga cagcgtcccg accaggaaca ggtagggcat gtcgaaggcg atgccgatga
     7921 ccgtgaccag cagggcctgg atcgcggcca ggatgacctg tgcccgcagg aagccgccga
     7981 aggcacgcgc cacgctgcgc tccaggatct gcatctcgtc ggagtagcgg cgcggcacgg
     8041 cgcggttgat cttggccacg atccggtcgc ggtccaggac catgtagagc gacaggatga
     8101 ggatgagcac cagcgagccc agcgtggcca gcgtggcgcc ggcgatctcc tcagcctgct
     8161 cgaagatggc cccggccacg ttgccgacct gccgttgggc ggactggaag agttcgacga
     8221 ggtccacctg gaatcggtcc aggccgaagg cctgctgggt ctcgagcagc tgcttctcga
     8281 tctgccgcgc ggtgccgggg aactccacgg tgaccagcgt cacctcctgg gtgatcgacg
     8341 aaccgacgta gaacagcacg aagcccaggg tcagcagcgc caggccgtag gcgatggcca
     8401 ccgccagcgg gcggggcagc ggcagcccct cgtccaggaa gcgcaccacc ggcgacatga
     8461 cgaaggccag cagccaggcc aggaagacga tcaaaatgat ctggctgaag ccgcccagga
     8521 atcccagcgc ctcctgcgcg accaggatcg cgaagtaggc cgaccccagg atgaggaaga
     8581 cgagcagcca gcgccgctcg cggggacccg atgcggccag gggtcccgga tcggccatct
     8641 gtctcaggcc cgccggccgt ctggaccgat gcccaccgcg gcccgcacct cgcgcatcgt
     8701 ctcctccagg attggccgca ccttggccgc accggcagcc agcaccgcct caacgtggcc
     8761 ggggtccgcc gccatctcgt cccgccgctg gcgcatgggc cggaagaact cgacgatgcg
     8821 ctcggccagc agctgcttgg tatcgacgca gccggtacga gccgtccgct caccgtcctg
     8881 gatctgcagg taatcgccgc cgaagaaccg atgcagctgg cacacgttgc agatctccgg
     8941 tcgcccgggg tcgctgcgct tgatgcgctg cgggtcggtc accatgctca tcaccagccg
     9001 ccgaatctca tcgggctcgg cgaagatcgg gatcgtgttg ttggcgctct tgctcatctt
     9061 gcgggcgccg tccagcccga gcacggtggg cgcatcggtg aacaccgcct gcggttccgg
     9121 gaagatctca ccgtagcggc ggttgaaggc gcgcacgatc tcgcgcgaaa gctccaggtg
     9181 cgctgcctgg tccttgccaa ccggcacccg cgtggccttg tagatgacga tatcggccgc
     9241 ctgcaggacc gggtagctga gcagcccgtt gctgttctcg atgccctcgg cgcgcttctc
     9301 cttgtacgtc ggcgtgcgct ccagccaggt gaccggcacc accgtgttga agatccactg
     9361 cagctcggcg tgctcgacga ggtccgactg ccggacgatg gcgcagcggt ccgggtccag
     9421 gcccactgcc agcaggtcga ggaccatgtc gtgggtcagc tggcggagct tcgccccgtc
     9481 gtgaaccgtg gtcagggcat ggaggtcgac gatggcgtag atcgcgtcgt actcgtcctg
     9541 catggcgacg tagttcgcct gcgcccccag gaagttgccc aggtgcgagg cgccggaggg
     9601 ctggatcccg gagaagacac gctctcgggt ggccggcgcc gtggtggtgg gggcgggagc
     9661 ggcctggctc atgggcagcg agtgtaaccc cataatgtcg ggcatgaccg atgcctccgc
     9721 ctggcaacgt gccgccgatc cagcgcggcg cataggccat gtcgtggagt ttcacccgtc
     9781 gatcggctcg accagtgacc gagcccgcga gctgctggcc gccggtcagg agggggtggc
     9841 cgtggtcgcc gatgagcaga ccgccgggcg cggacgccgc ggccgaagct gggcctcgcc
     9901 gcccggcgtc aacctgatgg tcagcgtggc cttccgtccc aacctcgaac ctgaggggct
     9961 ggggcacctg ggcctggggg cggccctggc cgtgcgcgct gcatgcatga ccgtggcgcc
    10021 cacaacccgc atctggttgc gctggcccaa cgacctggtg gccgacgacg gaggcaagct
    10081 ggccggcgtg ctggtcgaga cctcgatcgc cgagggacag ccggtggacg cggtggtggg
    10141 gatggggatc aacgtcaact gggcgcgcgg cgagatgccg gccgagatcg gggcccgcgc
    10201 gacctcgctg ggcgagctgg gcggaggcga ggtggaccgg gtggcgctgc tggccgccct
    10261 gcttcgggga gtggacggcg agctgagcgc cctcgagcgc ggtttgtcac cggtcccgcg
    10321 cgcccggaag gcatccgccc tggacggccg ctgggtgcag gtggagactg gcaacggcgc
    10381 cctgagcggc accgtggtgg gtatcgacga tgacggcgcg ctgctgctgg aggccgacgg
    10441 cgagcgccat gcgctgcacg tcggtgaggt ggttgccgtg cgcgacgtgg tgccggcgtg
    10501 accggccccc tgcacgagcc acgg
//
LOCUS       DATACA010000144         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2832351, whole genome shotgun sequence.
ACCESSION   DATACA010000144 DATACA010000000
VERSION     DATACA010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832351"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VHK28_02980"
     CDS             <1..417
                     /locus_tag="VHK28_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEX2141229.1"
                     /translation="GAFPYLVPQAYVSRARALLDEAAAGGRTRPALVVSLACRIEPDP
                     SAARAAARRYLDRYLGLPNYRANLLECGFDDGDLTKPGSDRLVDALVAWGDEPTVLGR
                     LRAMLEAGADHVALIPLTADGLHADRATMEALAPPW"
     gene            complement(445..648)
                     /locus_tag="VHK28_02985"
     CDS             complement(445..648)
                     /locus_tag="VHK28_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2141230.1"
                     /translation="MSVARVTELSCTSNESFEDAIRQGIERATKTLRQVRSAWVKEQR
                     VNVGRDGTVEFQANILVTFVLED"
     gene            complement(709..1743)
                     /locus_tag="VHK28_02990"
     CDS             complement(709..1743)
                     /locus_tag="VHK28_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2141231.1"
                     /translation="MSALRDPVTDLRRIAFLLEAANEPSYRVRAFRNAARTLAAMPAG
                     ELEERVTRGGLRELAGIGEVVERTILESARGEEPVYLRRLEATGGRMVAELGAELCAA
                     LRGDCHVHSDWSDGGATIREMAEAARELGREYMVLSDHSPTLTVANGLTAERLERQLD
                     EVAALNAELAPFRILTGIEVDILPDGSLDQSPELLSRLDVVVASVHSELRMEARFMTR
                     RMVTALADPNLDILGHCTGRMRTRRRNRPESTFDAEIVFAAAARFDKAIEVNSRPERL
                     DPPKRLLRLALEAGCKVSIDSDAHYPGQLAWLPYGCDRAAACGVQAGSVVNALGADAL
                     LDWAAAHEMA"
     gene            complement(1740..>2403)
                     /locus_tag="VHK28_02995"
     CDS             complement(1740..>2403)
                     /locus_tag="VHK28_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="phosphotriesterase homology protein; PhP; YhfV;
                     member of a family of proteins related to
                     phosphotriesterase (PTE); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HEX2141232.1"
                     /translation="GTGWYRQSYYPAEALIDRRNVDDLAAEMVAEFTDGVTGTGVHPG
                     IIGEIGTDKPWVSAQEERVHRAAARAARATGMAITTHAVMSPVGLDQLRIFEEEGVDP
                     ARVVIGHADSYPVLDHYLAILDRGANLEFDFIGHRFGTEEAAEPRLVHLMVELLERGY
                     AGQVLLSQDVCHNSQLKANDGFGYTYLQQHFLPTLRTAAVGEGEIRQMTVDNPRRILT
                     IA"
BASE COUNT          332 a          865 c          834 g          372 t
ORIGIN      
        1 ggcgcctttc cgtacctggt gccccaggcg tacgtgtcgc gcgcccgcgc gctcctggat
       61 gaagctgccg cgggcgggcg cacccgcccg gcgctcgtgg tcagcctggc ctgtcgaatc
      121 gagcccgacc cctctgccgc gcgggctgcg gcgcgacggt accttgaccg ctacctgggc
      181 ctgcccaact accgcgccaa cctgctggag tgcgggttcg acgatgggga cctgacaaag
      241 ccagggtcgg atcggctggt ggacgcgctg gtggcgtggg gggacgagcc aaccgtgctc
      301 ggcaggctgc gcgccatgct cgaggcgggc gccgaccacg tggcgctgat ccccctcacc
      361 gcggatggcc tgcatgccga ccgcgcgacc atggaggccc tggctccgcc ctggtagccg
      421 gtgccctccc cgccgggtcc gaggtcagtc ctccaggacg aaggtcacca ggatgttggc
      481 ctggaactcg acggtcccgt cgcggccgac gttcacgcgc tgctccttga cccaggcgct
      541 gcgcacctgg cgcagggtct tggtggcgcg ttcgatcccc tggcgaatgg cgtcctcgaa
      601 ggactcattc gaggtacagc tcagctcggt gacgcgtgcg acggacatgc gtgcctcctc
      661 atcctgatgg tgccggcccg atggtggggc caaagtatga ccgatgaatc acgccatctc
      721 gtgcgcggcc gcccagtcga gcagcgcatc ggcgccgagg gcgttgacga ctgagccggc
      781 ctgcacgccg catgccgcgg cccgatcgca gccgtacggc agccaggcca gctggccggg
      841 gtagtgcgcg tccgagtcga ttgacacctt gcaaccggcc tcgagcgcga ggcgaagcag
      901 gcgcttcggc gggtccagcc gctccggccg cgaattgacc tcgatggcct tgtcgaaacg
      961 tgccgccgcg gcgaagacga tctcggcgtc gaaggtcgac tccggccggt ttcgacggcg
     1021 cgtccgcatg cggcccgtgc agtggccgag gatgtccagg ttcgggtcgg ccagcgcggt
     1081 gaccatgcga cgcgtcatga aacgcgcctc catgcgcagc tccgagtgca cgctggcgac
     1141 gacgacgtcc agccgggaca ggagctcagg cgactggtcc agtgagccgt cggggaggat
     1201 gtcgacctcg atgccggtca ggatccggaa cggcgccagc tccgcgttga gagccgccac
     1261 ctcgtccagc tggcgctcca gccgctccgc cgtcaggccg ttggccacgg tcaacgtcgg
     1321 cgagtggtcg gacaggacca tgtactcccg tcccagctcg cgggcggcct cggccatctc
     1381 gcggatcgtg gcacctccgt ccgaccagtc ggagtggacg tgacagtcgc ctcgcagcgc
     1441 tgcgcacagc tccgccccca gctcggcgac cattcggccg ccggtcgcct ccagccggcg
     1501 gaggtagacc ggttcctccc cccgcgcgga ctccagaatg gtgcgctcga ccacctcgcc
     1561 gatgcccgcc agctcgcgca gcccgccacg cgtgacgcgt tcctccagct cccccgccgg
     1621 catggcggcc agcgtccgcg cggcgtttcg gaaggcccgc acccggtacg acggctcgtt
     1681 ggcggcctcg aggaggaagg cgatgcgccg caggtcggtg accgggtcgc ggagggcgct
     1741 cacgcgatgg tcaggatgcg gcgcgggttg tcgaccgtca tctgccggat ctcgccctcg
     1801 cccacggccg cggtgcgcaa cgttggcagg aagtgctgct gcaggtaggt gtagccaaag
     1861 ccgtcgttgg ccttgagctg gctgttgtgg cacacgtcct ggctcagcag cacctgcccg
     1921 gcgtagccgc gctccaggag ctccaccatc aggtgcacca gccgcggttc ggcggcctcc
     1981 tccgtcccga agcggtgccc gatgaaatcg aactccaggt tcgcgccgcg atccaggatg
     2041 gccagatagt ggtccagcac cggatacgag tccgcgtggc cgatgaccac gcgcgccggg
     2101 tccacgccct cctcctcgaa gatgcgcagc tggtccagcc ccaccgggga catcaccgca
     2161 tgggtggtga tggccatccc ggtcgccctg gcggctcgtg cggccgcgcg gtggacccgc
     2221 tcctcctggg cgctgaccca tggcttgtcg gtgccgatct cgccgatgat tccgggatgg
     2281 acgccggtgc cggtcacgcc atcggtgaac tcggcgacca tctcggcggc caggtcgtcc
     2341 acgttgcgcc tgtcgatgag cgcctccgcc gggtagtagg actgccggta ccaacctgtc
     2401 ccc
//
LOCUS       DATACA010000145         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_342205, whole genome shotgun sequence.
ACCESSION   DATACA010000145 DATACA010000000
VERSION     DATACA010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342205"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /gene="cphA"
                     /locus_tag="VHK28_03000"
     CDS             complement(<1..1809)
                     /gene="cphA"
                     /locus_tag="VHK28_03000"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015779327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX2141233.1"
                     /translation="MRIVETRVYRGPNYWSYDPAIKLVVDLGVLENFPSNTIRGFVEG
                     LLSVLPGVGEHSCGTGRAGGFSDRLREGTWLGHVAEHVALQLQREAGTEVGRGKTRGT
                     GEPGRYHVVYSYAEESVGLAAGRLAVRLVNHLVEADPAFDFGAEFEKLVLLAERAAFG
                     PSTQAILDEATLRDIPYIRLNEQSLVQLGHGVYQRRIRATMTSQTSSIGVDVAGDKKL
                     TNRLLAATGVPVPRSEVVRSEDEAAAAAASIGFPVALKPLDGNHGRGVMLKLEDEAAV
                     RTAYPIARGQSRSGALVVETFLVGNDYRCLVIGGVLRAVAQRVPAHVDGDGTHTLAEL
                     VEITNEDPRRGIGHERVLTRIKVDDEAVGYAREQGFEMTDVPPRAVRVYLKRTGNMST
                     GGISIDRTDEIHPDNAEVAELAARMVGLDIAGIDFVCPDISVPVRETGGGIVEVNAAP
                     GFRMHTHPTEGEAQYVAKPVIDMLFAPGSPSRIPIVAVTGSNGKTTTARMIAHVMKGM
                     GRKVGMTSTDGIFVDGRVVRRGDMSGPRSASMVLQNPTVDFAVFEVARGGILREGLGY
                     QRNDVAVVLNVTGDHLGLGGITSIRQLAAVKQVVVEA"
     gene            complement(1914..>2931)
                     /locus_tag="VHK28_03005"
     CDS             complement(1914..>2931)
                     /locus_tag="VHK28_03005"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEX2141234.1"
                     /translation="RRADGMLPSMADQPASGKSRAGHLLLIGGAEDKLRQRQILSRFV
                     SLAGGSDGRVAVISTASSLGDEATELYRSLFLQLGIPDVRGLRPLSREDADDRTATEA
                     VEDATGIFMTGGNQLRLSSVIGGTALGRAIGERHRRGAIVAGTSAGASAISAHMVAFG
                     TGGGTPKQRMTQMSAGLGLLPGVIVDQHFEQRNRLGRLLALVAQSPSLLGIGIDEDTA
                     LLVSASGVGEVLGKGSVTILDPAKLQTDAYEVKRHRPMMVSGVILHSLPSGYRFDLRR
                     RRLLAPLRPVSASERDLAQLSAASNVTRRLIRRIAAEGADDRAPERARRRSVRTRAAE
                     EASE"
BASE COUNT          402 a         1051 c         1048 g          430 t
ORIGIN      
        1 ggcctcgacc acgacctgct tgacggcggc cagctgccgg atggaggtga tgccgcccag
       61 ccccaggtgg tcgccggtca cgttgagcac caccgccacg tcattgcgct ggtagcccag
      121 gccctcgcgc aggatgccgc cccgcgcaac ctcgaacacc gcgaagtcga cggtcgggtt
      181 ctggagcacc atggaggccg agcgcgggcc gctcatgtca ccccggcgca ccacgcgtcc
      241 gtcgacgaag atgccatcgg tggaggtcat ccccaccttg cggcccatgc ccttcatcac
      301 gtgggcgatc atgcgggcgg tcgtcgtctt cccattcgag ccggtgacgg ccacgatggg
      361 gatccgcgac ggggagcccg gcgcgaagag catgtcgatc accggcttgg cgacgtactg
      421 cgcctcgccc tcggtgggat gggtgtgcat ccggaagccg ggcgccgcgt tgacctccac
      481 gatcccgccg ccggtctcgc ggaccggcac gctgatgtcc gggcacacga aatcgatgcc
      541 ggcgatgtcc aggccgacca tgcgggccgc cagttccgcc acctcggcgt tgtcggggtg
      601 gatctcatcg gtccggtcga tgctgatgcc gccggtgctc atgttgccgg tgcgcttgag
      661 gtagacgcgc acggcccgag gcgggacatc ggtcatctcg aagccctgct ctcgcgcgta
      721 gccgacggcc tcgtcgtcga ccttgatgcg ggtcagcacg cgctcgtgac cgatgccgcg
      781 gcgggggtcc tcgttggtga tctccaccag ctcggccagg gtgtgcgtgc cgtcgccgtc
      841 cacgtgggcg ggcacccgct gcgccaccgc gcgcagcacg ccgccgatga ccaggcaccg
      901 atagtcgttt cccaccagga aggtctcgac caccagggcg ccggaccggg actggccgcg
      961 cgcgatcggg taggcggtgc gtactgcggc ctcgtcctcc agcttcagca tcacgccgcg
     1021 gccgtgattg ccgtccagcg gcttgagggc caccgggaaa ccgatgctgg ccgcggccgc
     1081 cgcggcctcg tcctcgctgc gcaccacctc cgagcggggg accggcacgc cggtggcggc
     1141 cagcaggcgg ttggtcagct tcttgtcgcc ggccacgtcg acgccgatgg agctggtctg
     1201 gctggtcatg gtggcgcgga tgcgtcgctg gtagacgcca tggcccagct gcaccaggga
     1261 ctgctcgttg aggcggatgt aggggatgtc gcgcagcgtg gcctcgtcca ggatggcctg
     1321 cgtggacgga ccgaaggcgg cccgctcggc gagcaggacc agcttctcga actcggcgcc
     1381 gaagtcgaac gccgggtccg cctcgaccag gtggttgacc agtcgcacgg ccagccggcc
     1441 ggcagccagg cccacgctct cctccgcgta ggagtagaca acgtggtagc ggcccggctc
     1501 accggtgccg cgcgtcttgc cgcggccgac ctccgtgccg gcctcgcgct ggagctggag
     1561 cgccacgtgc tcggcgacgt ggccgagcca ggtcccctcg cgcagccgat ccgagaaccc
     1621 gccggcacgg ccggtgccgc acgagtgctc gcccacgccg ggcaggacgc tgagcagccc
     1681 ctccacgaag ccgcggatcg tgttcgaggg gaagttctcg aggacgccca ggtcgaccac
     1741 cagcttgatg gccgggtcgt agctccagta gttcgggcca cggtagacgc gcgtctccac
     1801 gattcgcagc tccggcgccg ccttcctgcg gccggcgctg cgagccttgg ccgcccgggc
     1861 cttcgccgcg ctgtggccat ccttggccgc gccggcccga cgcctggtgg tggtcactcg
     1921 ctggcctcct cggcggcgcg ggtacggacc gaccgccgcc gggcgcgctc aggcgcgcgg
     1981 tcgtcggctc cctcggcggc gatgcggcgg atgaggcggc gggtcacgtt gctggccgcc
     2041 gagagctggg ccaggtcgcg ctcggaggcc gaaactggac gcagcggggc cagcagccgt
     2101 cgacggcgga gatcgaaccg ataaccggac ggcagcgagt gcaggatgac cccgctgacc
     2161 atcatcggcc ggtgtcgctt gacctcgtag gcatcggtct ggagcttggc cggatcgagg
     2221 atggtgaccg atcccttgcc gagcacctcg cccacgccgg atgcgctcac cagcagcgcg
     2281 gtgtcctcgt cgatgccgat gcccagcagc gacggggact gggccaccaa agccagcagg
     2341 cgaccgagcc ggttccgctg ctcgaagtgc tggtcgacga tgacgccggg cagcagcccg
     2401 aggccggcgc tcatctgggt catgcgctgc ttcggcgtgc caccgccggt gccgaaggcg
     2461 accatgtgcg cgctgatggc cgatgccccc gcactggtcc cggccacgat cgcgccgcgg
     2521 cgatgccgct cgccgatggc ccggcccagg gcagtcccac cgatcacgct cgagagccgc
     2581 agctggttgc cgccggtcat gaagataccg gtcgcgtcct cgaccgcctc ggtcgccgtg
     2641 cggtcgtcgg cgtcctcgcg ggacagtggg cgcaggccgc gaacgtccgg gatgccaagc
     2701 tgcaggaaga gcgaccggta cagctccgtc gcctcgtcgc ccagcgagga agcggtggag
     2761 atcacggcca cccggccatc gctgccgccg gccagggaga cgaagcgcga caggatctgc
     2821 cgctggcgca gcttgtcctc ggccccgccg atgagcagca ggtgcccggc gcgcgacttg
     2881 ccggatgctg gttggtccgc catcgagggg agcataccgt ctgccctcct c
//
LOCUS       DATACA010000146         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_824121, whole genome shotgun sequence.
ACCESSION   DATACA010000146 DATACA010000000
VERSION     DATACA010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824121"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /gene="glgB"
                     /locus_tag="VHK28_03010"
     CDS             complement(<1..1862)
                     /gene="glgB"
                     /locus_tag="VHK28_03010"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX2141235.1"
                     /translation="MSTPTAQLGVPEAEAARLARGEHHEPHSLLGAHPVPGGTVVRAF
                     HPDADGVEILPPDGPPITMQPLGHGLWAALMPGTQPPVSYRLRFRFPDGNTWEHDDPY
                     RFLPTLGELDLHLIGEGNHERLWEVLGAHPRTVEGTAGVAFAVWAPNARSVRVVGDFD
                     RWDGRLLPMRSLGASGVWELFVPDIGPGELYKYEVVGRDGSLRLKADPLSFSMQVRPE
                     TASRVWDLDAFTWTDDAWMAERAARDPYRSPMATYEVHLGSWMRNPDGSWLGYRQAGE
                     RLAEHCRRFGFTHVELLPVAEHPFDGSWGYQVTGYYAPTARFGSPDDFRAMVDALHGA
                     GIGVLLDWVPAHFPNDDFALRRFDGTALYEHADPERGEHPDWGTLIFDYGRPEVRNFL
                     VANALFWLDRYHIDGLRVDAVASMLYLDYSRQAGEWKPNRFGGNENLEAIAFLREMNE
                     RVYAAFPGAVTIAEESTAFPGVSRPTYLGGLGFGFKWDMGWMHDTLAYFAEDPIHRRY
                     HHNRLTFRGLYLSSEQYVLPLSHDEVVHGKGSLLGKMPGDEWRRFANLRTLFGYMYTQ
                     PGKKLMFMGGDIAQWREWNHDAELDWQLLNNPYHEGVRHWLRDLNHFYRSGLATV"
     gene            2200..>3095
                     /locus_tag="VHK28_03015"
     CDS             2200..>3095
                     /locus_tag="VHK28_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2141236.1"
                     /translation="MGLFDGLLGLLSHDVGIDLGTANTLVTVRHRGIVISEPSVVAMD
                     TRTKRVLAIGAEAKRMVGRTPANIVAIRPLRDGVISDFDVTEQMIRYFIQKVHDRYAR
                     IPRPRVLVGIPSGVTEVEKRAVRDATINAGARWARLIEEPMAAAIGAGLPVSEPSGSL
                     IVDIGGGTTEVAVISLGGIVVSRSVRIGGDEMDADIVSFARREYNLLMGERTAEEIKI
                     AVGSAYPVPGDHTVTFRGRDLLTGLPRSVEVGSEQIREALEPSVVQIIDAIKETIEET
                     PPELVADIMDQGIVLAGGGALLA"
BASE COUNT          478 a         1046 c         1120 g          451 t
ORIGIN      
        1 accgtagcga ggccgctgcg gtagaaatga ttgaggtccc gcagccagtg gcgcacacct
       61 tcgtgatacg ggttgttcag gagttgccag tccagctcgg cgtcgtggtt ccactcgcgc
      121 cactgcgcga tgtcaccgcc catgaacatc agcttcttgc cgggctgcgt atacatgtag
      181 ccgaagagcg tgcgcaggtt cgcgaagcgg cgccactcgt cgccgggcat cttgccgagc
      241 agggagccct tgccgtgcac cacctcgtcg tgagagaggg gcagcacgta ctgctccgac
      301 gacaggtaga gcccgcggaa ggtgagccgg ttgtggtggt agcggcggtg gatggggtcc
      361 tcggcgaagt aggccagggt gtcgtgcatc cagcccatgt cccacttgaa gccgaagccc
      421 aggccgccca ggtaggtcgg ccgtgacacg ccggggaagg cggtcgactc ctcggcgatg
      481 gtgaccgctc ccgggaaggc ggcgtagacg cgctcgttca tctcgcgcag gaaggcgatg
      541 gcctccaggt tctcgttgcc gccgaagcgg ttcggcttcc actctccagc ctggcgcgag
      601 tagtcgaggt acagcatgga ggccaccgca tccacccgca ggccgtcgat gtggtagcgg
      661 tccagccaga agagcgcgtt ggcgaccagg aagttgcgga cctccggccg cccgtagtcg
      721 aagatcaggg tcccccagtc ggggtgctcg ccgcgctccg ggtcggcgtg ctcgtagagg
      781 gccgtgccgt cgaagcggcg cagggcgaag tcgtcgttcg ggaagtgggc cggcacccag
      841 tccagcagga cgccgatgcc ggcaccgtgc agcgcgtcca ccatcgcgcg gaagtcatcg
      901 ggcgagccga agcgcgcggt cggggcgtag tagccggtca cctggtagcc ccatgacccg
      961 tcgaacgggt gctccgccac cggcagcagc tccacgtgcg tgaagccgaa ccgccggcag
     1021 tgctctgcca gcctctcccc ggcctgccgg tagcccagcc acgacccgtc cgggttgcgc
     1081 atccaggagc ccaggtggac ctcgtaggtc gccatcggag accgatacgg atcgcgcgcc
     1141 gcgcgctccg ccatccaggc atcatcggtc caggtgaacg cgtccaggtc ccagacccgc
     1201 gacgcggtct cgggtcggac ctgcatgctg aagctcagcg gatcggcctt cagccgcagg
     1261 ctgccgtcgc ggccgacgac ctcgtacttg tagagctcgc cgggaccgat gtccggcacg
     1321 aacagctccc acacccccga cgcgcccagc gagcgcatgg gcagcaggcg tccgtcccag
     1381 cggtcgaagt cgccgaccac ccgcacgctg cgggcgttag gggcccagac ggcgaatgcc
     1441 accccggccg ttccctccac ggtgcgtggg tgggcaccga ggacctccca caatcgctcg
     1501 tggtttccct ccccgatgag gtgcaggtcc agctcaccca gggtgggcag gaagcggtac
     1561 gggtcgtcgt gctcccaggt gttgccgtcc gggaagcgga agcgcagccg gtagctgacc
     1621 ggtggctgcg tcccgggcat cagcgccgcc cacaggccgt gccccagcgg ctgcatggtg
     1681 atcggcggtc cgtccggcgg caggatctcg accccgtcgg catcggggtg gaaggcgcgc
     1741 accaccgtgc cgcccggcac agggtgggcg ccgagcaggg agtgcggctc gtggtgctcg
     1801 ccgcgcgcca ggcgagcggc ctcggcctcc gggacgccga gctgggcggt gggcgtggac
     1861 atgggtctaa ccatagtggt atgtgccagc acgtttcgtg cgaggcctgt accccgccga
     1921 ctggtttcga cgaggagccg ggccgaatcg aaggggatgg ggcggctccg tcgccgttgg
     1981 cgggccgggg tgccatatcg gcagaacgca cgcgcggcgg ccccggggtc aactcctgcc
     2041 cgagctttcg ggcagccggg tggtcgcagg aggggcggga gccgtgtgct agggtttggc
     2101 ccgcgccgac gaggtgccgg cgcgcaccgg tagcagaacc tgcgagcgac ggccctgcgt
     2161 gccacgggcc cctcgcttca gccacctggc ggaggcaact tgggactctt cgacggcctg
     2221 ctcgggctcc tgagccacga tgtcggcatc gacctcggga cggcgaacac gctggtcacg
     2281 gtgcggcatc gcggcatcgt catcagcgag cccagcgtgg tggccatgga caccaggacc
     2341 aagcgggtgc tggccatcgg tgccgaggcc aagcgcatgg tgggccgcac gccggccaac
     2401 atcgtcgcca tccggccgct gcgcgacggg gtcatcagtg acttcgacgt caccgagcag
     2461 atgattcgct acttcatcca gaaggtgcac gaccgatatg cccgcatccc ccggccgcgg
     2521 gtcctggtcg gcattccatc gggcgtgacc gaggtcgaga agcgcgccgt ccgcgacgcc
     2581 accatcaacg ccggcgcccg ctgggcccgc ctcatcgagg agcccatggc ggccgccatc
     2641 ggtgccggcc tgccggtatc cgagccgtcg gggagcctga tcgtggacat cggcggcgga
     2701 accaccgagg tggcggtcat cagcctgggg ggcatcgtgg tcagccgcag cgtgcgcatc
     2761 ggcggcgacg agatggacgc cgacatcgtc agcttcgcgc ggcgggagta caacctgctg
     2821 atgggagagc gcaccgccga ggagatcaag atcgcggtcg gctccgccta cccggtcccc
     2881 ggcgaccaca cggtcacctt ccgcgggcgc gacctgctga ccggcctgcc acgcagcgtg
     2941 gaggtcggca gcgagcagat tcgcgaggcg ctggagccgt cggtggtgca gatcatcgat
     3001 gccatcaagg agacgatcga ggagacgccg cccgagctgg tggccgacat catggaccag
     3061 ggcatcgtgc tggccggcgg tggcgcgttg ctggc
//
LOCUS       DATACA010000147         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1948381, whole genome shotgun sequence.
ACCESSION   DATACA010000147 DATACA010000000
VERSION     DATACA010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948381"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHK28_03020"
     CDS             complement(<1..188)
                     /locus_tag="VHK28_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141237.1"
                     /translation="MFRRMSPEERAARSARVARDVLMGVAPGMRTGRRFFSKVPSDPR
                     CKLCASPFAGPVAPVLGL"
     gene            390..1079
                     /locus_tag="VHK28_03025"
     CDS             390..1079
                     /locus_tag="VHK28_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141238.1"
                     /translation="MNRRLRGLGIGLVVIGIAFVAAGGFAFMKTQEGAASLQAFSAAQ
                     DVQLSYNEEGQLVDRGETAGAEAIMALLTDEWGYPVVSAELNPNDPLVNTASEYMYQM
                     ATIAYHTLHGTQTVELTEAVEYNGETFAPGTYEFAVDGRYWTDFDRSHPIEGPAREMA
                     WTGTAHALIAELGVGTMTAQALQLGLALAGLFAGIGATVLFTGAGLVWATRVETVPAP
                     AFQHATVGTPA"
     gene            complement(1325..2146)
                     /locus_tag="VHK28_03030"
     CDS             complement(1325..2146)
                     /locus_tag="VHK28_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141239.1"
                     /translation="MSALRLSLAPILALALLCLSAGAAVGREQPTTDARGGDSRSLTG
                     YDISYPQCGARYPTDVAFGIVGVNGGRVYSENPCLGAGGNGASQLAWAGLNAELYMNT
                     GNPGPELSSYWPVGQASPRVCSADDPDTADCAYDYGWNAAADAYAVAVRAYISLGWAS
                     PDAERTPVANRWWLDVETANSWRSDASLNVAALQGMVDFLESMEVESVGFYSVDGMWD
                     SITGGTDAFADRASWVAGASTLRGARANCDREPFTGGWVELTQYFAKGFDANHRC"
     gene            complement(2243..>3037)
                     /locus_tag="VHK28_03035"
     CDS             complement(2243..>3037)
                     /locus_tag="VHK28_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX2141240.1"
                     /translation="TPPRVRGALVLGISQSGRSPDVVEVVSEARRQGQLTAAITNDPQ
                     SPLAAAAEHVMALRAGPEESVAATKTYVAELLAVAMLAAGLSEDVRHADALRHVPDTI
                     GRALLSNADAEAAAEAHRSMDEAVILGRGFNLSTALEWALKLKELAYVRAHAYSTADF
                     QHGPAASLAPGGDLLAVSAHGPLETELIGVLQELATSRAARILQVSASPAELPGGTIR
                     FPDDLPEWLSPLAAIVPIQLFCYHLARAKGLDFEAPRGLRKVTLTQ"
BASE COUNT          504 a         1036 c         1069 g          428 t
ORIGIN      
        1 atcaggccca ggaccggggc gaccggtccg gcgaacggtg atgcgcacag cttgcaacga
       61 gggtcggacg gcaccttgct gaagaaccga cggccggtgc gcatgccggg cgcgactccc
      121 atcagcacgt cccgggctac gcgcgcgctg cgcgccgcac gctcctcggg tgacatccga
      181 cggaacaccc gaccattgtc cctgcctcgg caagcagtga gccggacggc atgtcggcgg
      241 ggtgaccagt ggcccgtcgg gcgggggccg ctgggctgcg gaatgggtcc acctgtcacg
      301 tgtgccgaat ggggcgcaga ccgcaaagtg cctgcatcgg gagaccgata cgaaagcaac
      361 aacaacgaac cgaaaggcag gcacactgaa tgaatcgtcg tttgcgagga ctgggcatcg
      421 gacttgtggt catcggcatc gccttcgtgg cagccggtgg cttcgcgttc atgaagaccc
      481 aggaaggcgc cgcgtcgctc caggcgttca gcgcagcgca ggacgtccag ctcagctaca
      541 acgaagaagg ccagctcgtc gatcgcggcg aaaccgcggg ggccgaagcc atcatggcgc
      601 tgctcaccga tgagtggggc taccccgtcg tcagcgccga gctgaacccg aacgacccgt
      661 tggtcaacac cgccagcgag tacatgtacc agatggcgac catcgcctac cacaccctgc
      721 acggcacgca gacggtcgag ctgaccgagg cggtcgagta caacggcgag accttcgccc
      781 ctggcaccta cgaattcgca gtcgacggcc gctactggac cgatttcgac cggtcgcacc
      841 cgatcgaggg cccggcgcgt gagatggctt ggaccggcac cgcacatgcc ctgatcgcgg
      901 agctcggcgt gggcaccatg accgcgcagg ccctgcagct gggcctggcc ctggccggtc
      961 tcttcgccgg aatcggcgca acggtcctct tcaccggcgc cggcctggtg tgggccaccc
     1021 gggtcgagac cgtcccggct cccgccttcc agcacgccac ggtgggcact cccgcctgac
     1081 gctgatcttg cggggcggct gccgccccgc atccctagcc cacggcgagg ccccattgag
     1141 ggtctcgccg ttcggctatc cacgggacgg ctgtaacgga caggaagtgg ccgtttatgc
     1201 gtggggcgac tggttcggcc aggctggcag gccgatgttg gctggctgag tcgtgcggcg
     1261 ggtaggacgg ccacgatgtg gccgatacac ccgtggagtg aatacgcggc aggagtgccg
     1321 cggctcagca gcggtggttg gcgtcgaacc ccttggcgaa gtattgcgtc agctccaccc
     1381 agccgccggt gaacggctcc cgatcgcagt tggcgcgagc accgcgcaag gttgacgcgc
     1441 cggcgaccca gctggcgcgg tcagcgaagg catcggtgcc gccggtgatg ctgtcccaca
     1501 tgccgtcaac gctgtagaag cccaccgact cgacctccat cgactccagg aagtcgacca
     1561 tgccctgcag agccgccacg ttcagggagg catcgctccg ccaggagttg gcggtctcca
     1621 cgtccagcca ccatcggttg gcgaccggcg tgcgctcggc atcgggagag gcccagccaa
     1681 ggctgatgta ggcgcgcacg gcgacggcgt aggcatcggc cgcggcattc cagccgtagt
     1741 cataggcgca gtcggcggtg tcggggtcat ccgcgctgca gacgcgcggg ctggcctggc
     1801 cgaccggcca gtacgagctc agctccggcc ccggattgcc ggtgttcatg tacagctcgg
     1861 cgttgagtcc ggcccaggcg agctgcgagg caccgttccc gccggcgccc aggcaggggt
     1921 tctcgctgta gacccgcccg ccgttgacgc caacgatccc gaaggcgacg tccgtcggat
     1981 agcgcgcgcc gcattgcggg taagagatgt cgtagccggt cagcgagcgg gaatcgccgc
     2041 cacgtgcatc cgtcgtcggc tgctcgcgac cgaccgcggc gccggcggac aggcacagca
     2101 atgccagggc caggattggg gccagggaca gacgaagggc gctcatgggc acgggcacgc
     2161 tcggagcatc gggtcacgca ggatagaacg tacgtgcgga acgggcaagc gagcgagcgg
     2221 ctgccggcga ctgagcgccc gcctactgcg tgagcgtgac cttacgcaag ccgcgcggcg
     2281 cctcgaagtc cagccccttg gcccgggcca ggtggtagca gaagagctgg atgggcacga
     2341 tggccgccag cggcgacagc cactcaggca ggtcatccgg gaagcgaatc gtcccgcccg
     2401 gaagctcggc gggcgaggcg gaaacctgca ggatgcgcgc ggcgcgtgat gtggcgagct
     2461 cttgcagcac gccgatgagc tccgtctcga gcggtccgtg ggcggagacg gccagcagat
     2521 cccccccggg cgccaggctg gcagccggac catgttgaaa atcggcggtc gaataagcat
     2581 gcgcgcggac gtaggccagc tccttcagct tgagcgccca ctccagcgcg gtcgacaggt
     2641 tgaacccgcg gcccaggatg accgcctcgt ccatggaccg atgcgcctcg gccgctgcct
     2701 cggcgtccgc gttcgacagg agggcccgac cgatggtgtc cggcacgtgc cggagtgcgt
     2761 cggcatgccg gacgtcctcc gagaggccgg cggccagcat ggccaccgcc agcagctcgg
     2821 ccacgtatgt cttggtggcc gccaccgact cctccgggcc tgcccgcagg gccatcacgt
     2881 gctcggcggc agcagccagt ggcgactgcg ggtcgttggt gatggctgcg gtcagctgtc
     2941 cctggcggcg cgcctcgctc accacctcca ccacgtccgg ggagcgaccc gactggctga
     3001 tgcccagcac gagcgcaccc ctgacccgcg ggggcgt
//
LOCUS       DATACA010000148         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_20774, whole genome shotgun sequence.
ACCESSION   DATACA010000148 DATACA010000000
VERSION     DATACA010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20774"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1456
                     /gene="prfB"
                     /locus_tag="VHK28_03040"
     CDS             join(346..390,392..1456)
                     /gene="prfB"
                     /locus_tag="VHK28_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959056.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX2141241.1"
                     /translation="MKDEALRLARLVADTRCGFDLPAKETELAELTDRSADPNLWNDP
                     SAAQAMLRRAEELRDEIGAWRDLEARATALAEIAELAETDSEEGDGLEADLQRDLRAL
                     TADWNRMEGRLLMSDPYDERPAIVSVHAGAGGTESQDWAEMLLRMYLRWAERNRYRTE
                     ILDQTEGEEAGLKSVTVSVEGRWAYGRLKSERGVHRLVRISPFDSNKRRHTSFALVEV
                     MPEVEDAPDIEIRDDDLRVDTYRASGAGGQHVNKTDSAVRLTHLPTGIVVTCQNERSQ
                     AQNRERAMAVLRAKLVERAQEEREVELARLRGQHVEAGWGNQIRSYVLQPYTMVKDLR
                     TGVETSNPSAVLDGEIDPFIEGYLRWRIGGDGAAA"
     gene            1516..2193
                     /gene="ftsE"
                     /locus_tag="VHK28_03045"
     CDS             1516..2193
                     /gene="ftsE"
                     /locus_tag="VHK28_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX2141242.1"
                     /translation="MIDVGMTYPNGKVALADVNVSIPSGDFVFLVGPSGAGKSTFIRL
                     LIREQLPTSGRVVVAGRDLSRMRRGEVHKLRRRIGIVFQDFRLLPAKTVYENVAFALD
                     VTGAPRSEIRQRVPQLLNLVGLQEHNDHLPTQLSGGEQQRVAIARALVHDPAMLIADE
                     PTGNLDPVTSWEIIQLLIQINGLGTTVLMATHNQEIVNAMRRRVLALEHGTLVRDESG
                     AAYEREY"
     gene            2198..3091
                     /locus_tag="VHK28_03050"
     CDS             2198..3091
                     /locus_tag="VHK28_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEX2141243.1"
                     /translation="MGFVRFVAFAVVRAWQGFWRNAMMSLAATATVILMLVLLSGLFI
                     VISGLNSGLEFIESKVGVTARLVGGLPDSQRDVVLAYAESLDGVNEVIYVSPELAMDR
                     LREYYQGLGQELELGGADIELYASMEISLDDPDFSGSVAAALEDRPEVERITTRQADY
                     EKLLGIIGVIRTVGIVALALVGLTVLFMIVNTIRIAVYSRANEIEIMRLVGASDSFIR
                     WPFILEGILCGLIGAVVTVILVAVVWNPIQPVMVQVFQMPTAVSAQFLATLSGVVLGV
                     GLLVGALGSWISVRANLSSAA"
BASE COUNT          527 a         1066 c         1109 g          478 t
ORIGIN      
        1 ccggggcgcg gtgatcccgg gatcgacaca gctggagggc gttcgtgaag cagctgaccg
       61 gcccaggcag gggtcaggca agcacccaga ccaccccctc aaccccggca gcagcccttc
      121 cgctggtaac ccgattcaga gaccccgaag aaccctatga agacctgatc atgtgcgagc
      181 gcgactgcat gcgctgcagc tacgcgctca cgctggactg cgacgggaac tgtggctcct
      241 gccctcgcca gtcatactgt ccctgcgtga acccggccaa ggtgcgaacc aagaacgcgc
      301 gcctggccga agtggagctc cccatggtgg cgacccttgg aagcaattaa ggacgaggcc
      361 ctgagactgg cgcgcctcgt ggccgacacg gcggtgcggc tttgacctgc ccgccaaaga
      421 gaccgaactg gcggagctga ccgaccggtc ggctgacccc aacctctgga acgatccatc
      481 cgcggcacag gcgatgctgc gccgcgcaga agagctgcgc gacgagatcg gtgcctggcg
      541 cgacctcgag gcgcgcgcca cggcgctggc cgagattgcc gagcttgccg agaccgacag
      601 cgaggaggga gacggcctgg aggccgatct ccagcgtgac ctccgcgccc tgaccgccga
      661 ctggaaccgg atggaggggc gcctcctgat gtccgatccg tacgacgagc ggccggcgat
      721 cgtatccgtc catgcgggag ccggcgggac ggagagccag gactgggcgg agatgctgct
      781 gcgcatgtac ctgcgctggg cggagcgcaa ccggtaccgg accgagatcc tggaccagac
      841 cgagggcgag gaggccggcc tcaagagcgt gacggtcagc gtcgaggggc gctgggcgta
      901 cgggcggctg aagagcgagc gtggggtgca tcggctggtg cgcatcagcc ccttcgactc
      961 gaacaagcgc cggcatacca gcttcgcgct ggtggaggtc atgccggagg tggaggacgc
     1021 gcccgacatc gagatccggg atgacgacct gcgggtggac acctatcggg ccagtggggc
     1081 aggtgggcag cacgtcaaca agaccgacag cgcggtccgg ctcacgcacc tgcccaccgg
     1141 catcgtggtc acctgccaga acgagcgcag ccaggcccag aaccgcgagc gtgccatggc
     1201 cgtgctgcgc gccaagctgg tggagcgtgc ccaggaggag cgcgaggttg agctggcccg
     1261 cctgcgcggg cagcacgtgg aagccggctg gggcaaccag atccgtagct acgtgctcca
     1321 gccgtacacc atggtcaagg acctgcgcac cggcgtggag acctccaacc ccagcgcggt
     1381 gctggacggc gagatcgacc cgttcatcga gggctacctg cgctggcgaa tcggcgggga
     1441 cggggccgcg gcatgatcga agcgctcgac cggccggtgg tcgccaccga gaccaccgcg
     1501 cagccggtca tcagcatgat cgacgtgggg atgacctacc ccaacgggaa ggtggcgctg
     1561 gccgacgtca acgtgtcgat accgtccggc gacttcgtct tcctggtggg gccgtcggga
     1621 gcgggcaagt ccaccttcat ccggctgctg atccgcgagc agctgcccac ctcgggtcgc
     1681 gtcgtggttg ccggccgcga cctgtcgcgc atgcgccgcg gcgaggtgca caagctccgc
     1741 cgccggatcg ggatcgtgtt ccaggacttc cggctgctgc cggccaagac ggtgtacgag
     1801 aacgtggcct tcgccctgga cgtcaccggc gcgccgcgct ccgagatccg gcagcgggtg
     1861 ccgcagctgc tgaacctggt cgggctgcag gagcacaacg accacctgcc cactcagctg
     1921 tccggcggcg agcagcagcg agtggccatc gcccgcgccc tggtccatga cccggccatg
     1981 ttgatcgccg acgagcccac cgggaacctc gacccggtca cctcgtggga aatcatccag
     2041 ctcctgatcc agatcaacgg gctgggcacc acggtgctga tggccaccca caaccaggag
     2101 atcgtgaacg ccatgcgccg ccgcgtgctg gcgctggagc acggcaccct ggtccgcgac
     2161 gagtctggcg cagcctacga gcgcgagtac tagaccgatg ggattcgtcc gcttcgtggc
     2221 cttcgccgtg gtgcgcgcct ggcagggctt ctggcgcaac gcgatgatga gcctggccgc
     2281 gaccgccacc gtgatcctga tgctggtcct gctgtccggg ctgttcatcg tgatcagcgg
     2341 cctgaactcc gggctggagt tcatcgagag caaggtgggc gtcacggcgc ggctggtcgg
     2401 cgggttgccg gacagtcagc gcgacgtggt gctggcctac gccgagtcgc tcgacggcgt
     2461 caacgaggtg atctacgtca gccccgagct ggccatggac cgcctgcgcg agtactacca
     2521 gggcctcggc caggagctcg agctgggcgg cgccgacatc gagctctacg ccagcatgga
     2581 gatcagcctg gacgacccgg acttctccgg ctcggtggcg gccgccctgg aggaccgtcc
     2641 ggaagtggag cgcatcacca cccgccaggc ggactacgag aagctgctgg ggatcatcgg
     2701 cgtcatccgc acggtcggca tcgtggccct ggcgctggtg ggcctgaccg tcctgttcat
     2761 gatcgtgaac acgatccgca tcgctgtcta ctcgcgcgcc aacgagatcg agatcatgag
     2821 gctggtgggc gcctcggact ccttcatccg ctggccgttc atcctggagg ggatcctgtg
     2881 cggcctgatc ggcgcggtgg tcaccgtgat cctggtggcc gtggtctgga accccatcca
     2941 gccggtcatg gtccaggtct tccagatgcc caccgcggtc agcgcccagt tcctggccac
     3001 cctctccggc gtggtcctgg gcgtggggct gctggtcgga gcgctgggca gctggatcag
     3061 cgtgcgcgcc aacctctcgt cggcggcctg atcccccctt gacgccggtt attgaaacgt
     3121 aaccggcgcg taacggactt cacggtgccc ggtgctagac tgcccgcctt catgaccgat
//
LOCUS       DATACA010000149         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1547589, whole genome shotgun sequence.
ACCESSION   DATACA010000149 DATACA010000000
VERSION     DATACA010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547589"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1263)
                     /gene="recA"
                     /locus_tag="VHK28_03055"
     CDS             complement(193..1263)
                     /gene="recA"
                     /locus_tag="VHK28_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX2141244.1"
                     /translation="MDAPVAQAQSQTPPERNGERSRAVDAAILQIEKQFGRGSIMRLG
                     DKGAHQNVDVIPTGSIALDLALGVGGVPRGRITEIFGPESSGKTTLCYHVAANAQRNG
                     GIAAFIDAEHALDPRYAANVGVNVDDLLVSQPDTGEQALEIAETLIRSNGVDVVIVDS
                     VAALVPRAEIEGEMGDSFVGLQARLMSQALRKLTGAINRSNTALVFTNQLREKIGVMF
                     GNPETTPGGRALKFYSSVRLDIRRIETLKAGTDAVGSRTRVKVVKNKVASSFRIAEFD
                     ILYNEGISREGGLLDVGIAAGILSKTGAWFNYGETRLGQGRENAREFLRANPELAAKV
                     EDEVRGKVASIEVGVEGISEAQ"
     gene            complement(1309..1482)
                     /locus_tag="VHK28_03060"
     CDS             complement(1309..1482)
                     /locus_tag="VHK28_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141245.1"
                     /translation="MAKGRDRPRHQEKRKPKAKAPKAPPVSAFEPPMSVEVIKPRRKP
                     RTEPSGEPSGEEE"
     gene            complement(1550..2692)
                     /gene="tgt"
                     /locus_tag="VHK28_03065"
     CDS             complement(1550..2692)
                     /gene="tgt"
                     /locus_tag="VHK28_03065"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX2141246.1"
                     /translation="MPAPVEFTFRPTRIGRARLGRLVTPHGAIDTPQFMPVGTQATVK
                     ALTPADLRAAGTQVILGNTYHLSLRPGHERIARLGGLHRFMGWDGPILTDSGGFQVFS
                     LAHRRRVDEDGVTFASHLDGSSHRITPESAIAIQEALGSDVAMALDQLVGPEEPSAQV
                     AKAMERTHRWAERCLAAHTRPDQALFGIVQGGVDPQLRRRSTQVVASLPFDGIAVGGL
                     SVGESKADMAAALDAVAATLGDDPRVRYLMGVGSPADFFTAIERGIDLFDCVLPTRVA
                     RNGQLWTSEGRLNIRNARFMDDPGPIDPACACEACQNHSRAYLAHLFRAEELLAYRLA
                     SVHNVTYTLDLMRRIRGALDDGSFASLRDVVAAQYGSIGQAAISRR"
     gene            2783..3451
                     /locus_tag="VHK28_03070"
     CDS             2783..3451
                     /locus_tag="VHK28_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141247.1"
                     /translation="MRLDDWPRNPYLNEELREWIRLHLRALGADDVAIFATTAGREED
                     ERRVLVATEVGLLDTWYAPRGSSARYALNVRLYPWQQVHGVDLRGETYRLWAHEHQSR
                     WRLRLARPALDAISDDAGVGRALAEFASACSVMAETGGQQISEDGHPGLAARSEAAAS
                     APASSAPSPQPTTPPAERVPPRIAGVPGADRPRPPIRVEPSLADEPALEVEQGVHAHP
                     PGRG"
     gene            complement(3461..>3748)
                     /locus_tag="VHK28_03075"
     CDS             complement(3461..>3748)
                     /locus_tag="VHK28_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX2141248.1"
                     /translation="TTSVRVLETAVSGKSASGWSDLYITPGHRFRVVDALVTNFHLPR
                     SSLLLLVTAFVQDGMGDTDAFAARDTLLAAYRVALEKGYRFFSFGDAMLIL"
BASE COUNT          509 a         1343 c         1310 g          586 t
ORIGIN      
        1 gtcctgggct ccggcgcgcc gcagccggcg ctcgacctcc cagcggctgc gcgggcgcgt
       61 ggccaggaag cgcgcggcga tctcgatggc ggcggggacg tcgggcggct cgcgcggctc
      121 gtcgcgcggc cggcggcgac cgcccgggcg gccccgagca ggcggcggtt cgctcatcga
      181 gcgacgccag cgttactggg cctcgctgat cccctccacc ccgacctcga tcgaggcgac
      241 cttgccgcgc acctcgtcct cgaccttggc cgccagctcc gggttggcgc gcaggaactc
      301 gcgggcgttc tcacgcccct ggcccaagcg ggtctcgccg tagttgaacc aggcaccggt
      361 cttggacagg atgccggccg ctatgccgac gtccagcagc ccgccctcgc ggctgatgcc
      421 ctcgttgtag aggatgtcga actcggcgat gcggaatgac gaggcgacct tgttcttcac
      481 gaccttgacg cgggtgcggc tgccgacagc atcggtcccg gccttgagcg tttcgatgcg
      541 ccgaatgtcg agccggacgc tggagtagaa cttcagcgcg cgtccaccgg gcgtggtctc
      601 cgggttgccg aacatcacgc cgatcttctc gcgcagctgg ttggtgaaga ccagcgcggt
      661 gttcgatcgg ttgatggccc ctgtcagctt gcgcagcgcc tggctcatga ggcgtgcctg
      721 gaggccgacg aagctgtccc ccatctcgcc ctcgatctcg gcgcgcggca ccagcgcggc
      781 caccgagtcg acgatgacca cgtccacccc gttggagcgg atgagcgtct cggcgatctc
      841 cagcgcctgc tcgccggtgt ccggctggct gacgagcagg tcgtccacgt tcacgccgac
      901 attggcggcg tagcgcgggt cgagcgcatg ctccgcgtcg atgaaggcgg cgatgccgcc
      961 gttgcgctgt gcgttggcgg ccacgtggta gcagagcgtg gtcttgccgc tcgactcggg
     1021 gccgaagatc tccgtgatcc ggccgcgtgg cacgccgccc acgccgagcg cgaggtccag
     1081 cgcgatggag ccagtgggga tgacgtcaac gttctggtgc gctcccttgt caccgaggcg
     1141 catgatcgag ccgcgcccga actgcttctc gatctggagg atggcggcat cgaccgcgcg
     1201 gctgcgctcg ccgttccgct cgggcggggt ctgggactgg gcctgggcca ccggggcgtc
     1261 catcggttct cgggctcctt ctactggtgc gtcccggcgg cgatcaggtc actcctcctc
     1321 gccgctgggc tcgccgctgg gttcggtgcg cggcttgcgc cgtggcttga tcacctcgac
     1381 cgacatcggc ggctcgaaag ccgataccgg gggagccttc ggcgccttgg ccttcggctt
     1441 gcgcttctcc tggtgacgcg gacggtctct ccccttcgcc atcttccgca gacctccgat
     1501 ctcgtcggtc gggcgcctac agcctgcccg atggtgcctt atcggcccct tatcggcggc
     1561 ttatcgcggc ctgcccgata ctcccgtact gtgccgcgac gacgtctcgc agcgatgcga
     1621 acgacccgtc gtccaacgcg ccgcggatgc ggcgcatcag gtccagagta taggtgacgt
     1681 tgtggaccga ggcgaggcga tacgcgagca gctcctcggc tcggaacagg tgcgccaggt
     1741 aggctcgaga gtggttctgg caggcctcgc aggcgcatgc cgggtcgatc ggtccggggt
     1801 catccatgaa gcgcgcattg cggatgttga gcctgccctc gctggtccac agctggccgt
     1861 tgcgcgcgac ccgcgtgggc agcacgcagt cgaagaggtc gatgccgcgc tcgatcgcgg
     1921 tgaaaaagtc ggccggtgag ccgaccccca tcaggtagcg gacgcgaggg tcgtcaccga
     1981 gcgtggcggc caccgcgtcc agcgcggcgg ccatgtctgc cttgctctcc cccaccgaca
     2041 ggccgccgac cgcgatcccg tcgaatggca acgatgccac gacctgcgtg ctccgccgcc
     2101 gcagctgcgg atccacgccg ccctgcacga tgccgaacag cgcctggtcc ggacgggtgt
     2161 gggccgccag gcagcgctcc gcccagcggt gggtgcgttc catggccttt gctacctgtg
     2221 cggacggctc ctccggcccg accagctggt cgagcgccat ggccacgtcg ctgcccagcg
     2281 cctcctggat ggcgattgcc gactccggcg tgatgcggtg cgacgatccg tccaggtggc
     2341 tggcgaaggt cacgccatcc tcgtccaccc ggcggcgatg cgccaggctg aagacctgga
     2401 acccgcccga atcggtgagg atcggtccat cccagcccat gaaccgatgg agcccaccaa
     2461 gccgcgcgat gcgctcgtgt ccgggccgca gcgacaggtg gtaggtgttg ccaaggatga
     2521 cctgggtgcc cgccgctcgc agatcggcgg gcgtcagcgc cttgaccgtg gcctgcgtac
     2581 ccaccggcat gaactgcggg gtgtcgatgg caccgtgcgg ggtgacgagc cgccccaacc
     2641 gggcacggcc gatccgggtt ggccggaacg tgaactcgac gggagctggc acgctcggag
     2701 aataggacct tggggttggc cctggccgct caacgcgata gtcttactcc gccgcccacc
     2761 tactgctggg gagagacgcc tggtgcgatt ggacgactgg ccccgcaatc cgtacctcaa
     2821 cgaggagctg cgggagtgga ttcgcctcca cctccgcgcc ctcggggccg acgacgtggc
     2881 gatcttcgcc accaccgccg gccgtgaaga ggacgagcgc cgcgtgctgg tcgccaccga
     2941 agtgggcctg ctcgacacct ggtacgcgcc gcgcggctcg tcggcgcgct acgccctgaa
     3001 cgtgcgcctc tacccgtggc agcaggtgca cggcgtcgac ctgcgcggag agacctaccg
     3061 gctctgggcg cacgagcacc agagccgctg gcgcctgcgg ctggcccgcc cggccctgga
     3121 tgcgatctcg gacgacgccg gcgtgggccg cgccctggcc gagttcgcgt ccgcctgctc
     3181 ggtgatggcc gagaccggcg ggcagcagat cagcgaggac gggcatcccg gcctcgccgc
     3241 tcgctcggag gccgccgcat cggcgccggc cagcagcgca ccatcacccc agccgaccac
     3301 gccgcccgcc gagcgcgtcc cgccccgcat cgcgggcgtg cccggggcgg atcgtccgcg
     3361 gcctccgatt cgcgtcgagc cgagcctcgc cgacgagccg gccctcgagg tggagcaggg
     3421 cgtccacgcc catcctcccg gtcgcggctg acgcgcgccg ctacaggatc agcatcgcgt
     3481 cgccgaagct gaagaagcgg tagcccttct cgagtgcgac gcggtaggcg gccagcagcg
     3541 tgtcgcgcgc cgcgaacgca tccgtgtccc ccatcccatc ctgcacgaag gcggtgacca
     3601 gcagcagcag cgagctgcgc ggcaggtgga agttggtgac cagcgcgtcg accacccgga
     3661 accgatggcc gggcgtgatg tacaggtcgc tccacccgct ggcggacttg ccgctgacgg
     3721 ccgtctccag cacccgcacg ctggtggt
//
LOCUS       DATACA010000150         1868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1868696, whole genome shotgun sequence.
ACCESSION   DATACA010000150 DATACA010000000
VERSION     DATACA010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868696"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VHK28_03080"
     CDS             complement(<1..555)
                     /locus_tag="VHK28_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX2141249.1"
                     /translation="MATRPAARGPVAQVLARPGPRRLALACAALAGLTAVLLVVQWAL
                     VAAVVSAVFAGRAAPGDLLGALAGALAAWLARAGLLALRELLAARASARTRHEVRSEL
                     VGKVLRLGPALMAGERAGELVTTATEGVAKLDGLVARLLPGAVWAAVLPPLLAGTVLV
                     LDPPSGALLLATGPLLVFFLWLVGT"
     gene            complement(555..1589)
                     /gene="cydB"
                     /locus_tag="VHK28_03085"
     CDS             complement(555..1589)
                     /gene="cydB"
                     /locus_tag="VHK28_03085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2141250.1"
                     /translation="MDLVSLWFWLIALTFTLYFFLEGFDFGVDILRPTLARNEAEERA
                     LTGTIGPFWDGNEVWIIAAAGLMFSTFPIWYGAVFSGLYPVFVAILLALLLRGVSFEY
                     RNQVDQQRWRDFWDWMSFLGSVIPSFLWGVVMAKIIEGLALDEDSRVVSPVTELFTPF
                     ALVGGCATLLLFVLHGANFLLLRLHQDTPLHARARTAALGWGALATVAIVAFVVMGYV
                     TEGLFEDFGVLPWLFPVAAFLTLASIWLALTLRRDTLAFVMSGLTILFATVTVFIALF
                     TRGVVLPSTLDPAFSLTLQESASQHYTLVLMTWVGAIFLPLIIGYQIWNYYVFRERVR
                     PDEGALDRGY"
     gene            complement(1592..>1868)
                     /locus_tag="VHK28_03090"
     CDS             complement(1592..>1868)
                     /locus_tag="VHK28_03090"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2141251.1"
                     /translation="TGWMVTEMGRQPFMVYGLLTVEEGVSANSFGEVLAGLVGLWVVY
                     LALIGLDVYLLTVTARAGIHRKPDAQLLAAPAPSYEGAGFQGYGRKD"
BASE COUNT          327 a          673 c          678 g          190 t
ORIGIN      
        1 ggtgccgacc agccagagga agaacaccag cagcggcccg gtggccagca gcagcgcgcc
       61 gctcggcggg tccaggacca ggacggtgcc ggccagcagc ggcgggagca ccgcggccca
      121 caccgcgccg ggcagcaggc gggccacgag gccgtcgagc ttggcgaccc cctccgtggc
      181 cgtcgtgacc agctccccgg cccgctcccc cgccatcagc gccgggccca gccgcaggac
      241 cttgcccacc agctcgctgc ggacctcgtg gcgggtccgc gccgacgcgc gtgccgccag
      301 cagctcccgc agggccagca ggccggcccg cgccagccac gcggccaggg ctccggcgag
      361 ggcgccgagc aggtcgccgg gggcggcccg gccggcgaac accgcgctga cgacggcggc
      421 gaccagcgcc cactgcacga cgagcaggac ggcggtgagc ccggccaggg cggcgcaggc
      481 cagcgccagc cggcggggtc cgggccgggc cagcacctgc gccacggggc cgcgcgccgc
      541 cgggcgagtg gccatcagta cccccggtcg agggcaccct cgtcggggcg cacccgctcc
      601 cggaagacgt agtagttcca gatctggtag ccgatgatca gcggcaggaa gatcgcgccc
      661 acccaggtca tcagcaccag ggtgtagtgc tggctggccg actcctgcag cgtcaggctg
      721 aacgccgggt ccagcgtgga gggcagcacc acgccccggg tgaacagggc gatgaacacc
      781 gtcaccgtgg cgaagaggat ggtcagcccg ctcatcacga acgccagggt gtcgcggcgc
      841 agcgtcagcg ccagccagat gctggccagg gtgaggaacg ccgcgaccgg gaagagccag
      901 ggcagcacgc cgaagtcctc gaacaggccc tcggtcacgt agcccatcac gacgaaggcc
      961 acgatggcca cggtcgccag cgctccccac ccgagcgccg ccgtgcgggc ccgggcgtgc
     1021 agcggggtgt cttggtgcag ccgcagcagc aggaagttcg cgccgtgcag cacgaacagc
     1081 agcagcgtgg cgcacccacc caccagcgcg aacggcgtga acagctcggt gaccgggctg
     1141 accacccggg agtcctcgtc cagcgcgagc ccctcgatga tcttggccat caccacgccc
     1201 cagaggaacg aggggatgac gctgccgagg aaggacatcc agtcccagaa gtcccgccag
     1261 cgctgctggt cgacctggtt gcggtactcg aaggacaccc cgcgcaggag cagagccagc
     1321 aggatcgcga cgaagaccgg gtagaggccg ctgaagaccg cgccgtacca gatggggaag
     1381 gtggagaaca tcaggccggc cgcggcgatg atccacacct cgttgccgtc ccagaagggg
     1441 ccgatggtgc cggtcagggc ccgttcctcg gcctcgttcc gcgccagcgt gggccgcagg
     1501 atgtccacgc cgaagtcgaa gccctcgagg aagaagtaga gggtgaacgt cagcgcgatg
     1561 agccagaacc acagcgagac gaggtccacc gtcagtcctt ccgcccgtag ccctggaagc
     1621 cggcgccctc gtagctgggc gcgggcgcgg cgagcagctg cgcgtcgggc ttgcggtgga
     1681 tgccggctcg cgcggtcacg gtgagcaggt agacgtcgag gccgatgagg gcgaggtaca
     1741 ccacccacag cccgaccagg ccggcgagca cctcgccgaa ggagttggcg ctgaccccct
     1801 cctcgacggt gagcaggccg tagaccatga agggctgccg gcccatctcg gtgaccatcc
     1861 acccggtg
//
LOCUS       DATACA010000151         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1466471, whole genome shotgun sequence.
ACCESSION   DATACA010000151 DATACA010000000
VERSION     DATACA010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466471"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VHK28_03095"
     CDS             <1..1764
                     /locus_tag="VHK28_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2141252.1"
                     /translation="GDYLGTPLEETLPVYMTVRDSERTPEVAMVAVVDQSGSMADCHC
                     TGDERDTATSGTRGFEKVDIAKEAILRAADALAPTDQLGVVAFDEGAHWALRTGPIDV
                     NGLQSSLGFRADGQTNIFAGLKAAYDDLVRNPAKLRHIILITDGWSQSGAYDQLLADM
                     KAAGITLSTIGTGGGSADILRRLAEQSGGRYYDAGDATTIPDIFLRETIRTAGEQIVE
                     EAFQPIPSAPSEILRGLDAGRLPALRGYNATTPKGTATVALLTGREDPLLAQWQYGLG
                     RAAAWTSDARQQWAGEWIGTPEYGTLLSQLVAWTLPPQDDQGIDVRFSPGREGQLNVD
                     VTSLDPDGAPRNFYRSVLRLVGPGLEPAQVVLEQVGPGHYAGSVRAEEPGAYLVRVAQ
                     TSPGAEAASRTLGMVSPAAEEFRRLGVDGEALAELAVAGGGRELVVDEEGQAAAAVWS
                     HDISAAAFPTPMWPCLLLLAMVLVPLDVGVRRVAISRSDLRRARHWAARRVGLVQKVP
                     EAVPGLAELQAARDRTRRRADRAPGRAEPVAPRAVPDAAPSVPLDVSESGPPPAAEAS
                     PAGTTETLAERLARQRQRSRR"
     gene            complement(1766..>2062)
                     /locus_tag="VHK28_03100"
     CDS             complement(1766..>2062)
                     /locus_tag="VHK28_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141253.1"
                     /translation="LLVDGSPVATGPGGEFRVEVPAGLLPRTVRVQATDELGNTAESA
                     MSVVAPLDYRRLPWIAIVVALTLAAAVMLYLRVPRPAAARLPSSTDEGTLEEID"
BASE COUNT          295 a          740 c          761 g          266 t
ORIGIN      
        1 ggcgactacc tcggcacgcc gctcgaggag acgcttccgg tgtacatgac cgtccgcgac
       61 agcgaacgca ccccggaggt ggcgatggtg gccgtggtcg accagtccgg cagcatggcg
      121 gactgccact gcaccggcga cgagcgggac accgccacca gcgggacgcg cggcttcgag
      181 aaggtggaca ttgccaagga ggcgatcctg cgcgccgccg atgcgctggc gcccaccgac
      241 cagctgggcg tggtcgcctt cgacgagggt gctcactggg cgctgcgcac cgggccgatc
      301 gacgtcaacg gcctccagtc gtcgctcggc ttccgggcgg acggccagac caacatcttc
      361 gccggcctga aggcggccta cgacgacctg gtccgaaacc cggccaagct gcgccacatc
      421 atcctgatca ccgatggctg gtcgcagtcc ggcgcctacg accagctgct ggccgacatg
      481 aaggcggccg gcatcacgct gtcgaccatc ggcactggcg gcggctcggc cgacatcctg
      541 cgccgcctgg ccgaacagtc gggcggccgc tactacgacg ccggcgacgc gaccaccatc
      601 ccggacatct tcctgcgcga gacgatccgc accgccgggg agcagatcgt ggaggaggcc
      661 ttccagccga ttccatcggc cccgtccgag atcctgcgcg ggttggatgc cgggcggctc
      721 ccggcgctgc ggggctacaa cgccactacg ccgaagggca ccgccacggt ggcgctgcta
      781 accggccgcg aggatcccct gcttgcccag tggcagtacg ggctgggccg cgctgcggcc
      841 tggaccagcg acgcgcggca gcagtgggcc ggcgagtgga tcggcacccc cgagtacggc
      901 acgctgctct cgcagctggt ggcctggacc ctgccgccgc aggatgacca gggcatcgac
      961 gtgcgcttct cgccgggtcg cgaaggacag ctcaacgtgg acgtgacctc cctggacccc
     1021 gacggcgcac cgcgcaactt ctaccgcagc gtgctgcggc tggttggccc cggactggag
     1081 cccgctcagg tcgtgctgga gcaggttggc cccggccact atgccggcag cgtgcgggcc
     1141 gaggagcccg gcgcctacct ggtgcgcgtg gcccagacca gcccaggcgc ggaggcggca
     1201 tcacgaaccc tgggcatggt gagtccggcg gcggaggagt tccggcgcct cggcgtcgac
     1261 ggcgaggcac tcgccgagct ggcggtcgcc gggggcgggc gcgagctggt ggtggacgag
     1321 gagggccagg cggcagccgc ggtctggtcc catgacatct cggccgccgc cttcccaaca
     1381 ccgatgtggc catgcctgct gctgctggcc atggtgctcg tgccgctgga cgtcggcgtg
     1441 cgccgggtgg cgatcagccg ctccgacctg cgccgtgcgc gccattgggc ggcacgccgc
     1501 gtggggctgg tgcagaaggt cccggaggca gtgcccgggt tggccgagct ccaggccgct
     1561 cgggaccgga cgcggcgacg ggccgatcgt gctcccggac gagcggaacc ggtggctccg
     1621 cgagccgtgc ccgacgcagc gccatccgtg ccgttagatg tgtccgagtc cggtccgccg
     1681 cccgccgcgg aggccagtcc ggccgggacc accgagacgc tggcagagcg gctggcacgc
     1741 cagcgccagc gcagccgccg ctgagctagt cgatctcttc gagggttccc tcgtcggtgg
     1801 aagacggcag ccgggccgcg gcggggcgcg ggacacgcag gtagagcatc acggcggccg
     1861 ccagcgtcag ggccaccacg atggcgatcc acggcagccg ccggtagtcg agtggagcga
     1921 ccacgctcat ggccgattcg gcggtgttgc ccagttcgtc cgtggcctgg acacggaccg
     1981 tcctgggcag caggccggcc gggacctcga cgcggaactc accgcccggc ccggtggcca
     2041 ccgggctgcc atccacgagc ag
//
LOCUS       DATACA010000152         1970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2833270, whole genome shotgun sequence.
ACCESSION   DATACA010000152 DATACA010000000
VERSION     DATACA010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833270"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VHK28_03105"
     CDS             <1..507
                     /locus_tag="VHK28_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX2141254.1"
                     /translation="PEMRTVETVRQLVYFVAEQDKAKALLELTRHFGFDRLLVFRHTQ
                     IGVDRLAATLKRLGHNVAALHGGMSQRDRTRTLADFKSGKIQFLVATNVASRGLDITD
                     LPFVLSYDIPEDADTYVHRVGRTARAGRKGTAITFVGEWDLDAWEQIREQVGAEIEEG
                     QLDVYGAG"
     gene            544..1257
                     /locus_tag="VHK28_03110"
     CDS             544..1257
                     /locus_tag="VHK28_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141255.1"
                     /translation="MAKELTLRPQLASPSDAVIQSVEWLFEPAWRGDRLIARMRAGRV
                     SLTDEDGQPTSRPDLDEVVEVLEPALRTEDAVIDGIWTAMPFVGEGSAARHLADAIEE
                     EGLTGEVPDPIENEPRRAFVAIDLVEIDGQLLHDVPYQERRRLLTAIVDEDIRVRVTP
                     AVRVPLHNWLLAWRAAGFSHYVAKHMNSRYRPGEVAEDWLLISTAERRGANPIGRLFG
                     MRPKKVLHIEDDGNPKVRR"
     gene            1518..>1970
                     /locus_tag="VHK28_03115"
     CDS             1518..>1970
                     /locus_tag="VHK28_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141256.1"
                     /translation="MLRFQSRASTAKRPREHAQEVPPLPSNPTTTPDSALDEKGLEEV
                     RRVGSADLMVGIPSFGNAETIGYVVRAAQAGMVQFFPDLKPVLVNSDGGSPDDTPRVA
                     VSTESPEYIEKIILVSSRHKLQRVTFTYQGTSGKGTAVRALFEGARELK"
BASE COUNT          327 a          700 c          682 g          261 t
ORIGIN      
        1 cccgagatgc gcaccgtgga gaccgtccgg cagctggtgt acttcgtcgc cgagcaggac
       61 aaggccaagg ccctgctgga gctgacgcgc cacttcggct tcgaccgcct gctggtcttc
      121 cgccacacgc agatcggcgt ggaccgcctg gcggccaccc tcaagcggct gggccacaac
      181 gtggcggcgc tgcacggcgg catgagccag cgcgacagga cgcggacgct ggccgacttc
      241 aagagcggca agatccagtt cctggtggcc accaacgtgg ccagccgcgg cctggacatc
      301 accgacctgc cgttcgtgct gtcctacgac atccccgagg acgccgatac ctacgtccac
      361 cgcgtcggcc gcaccgcccg cgccgggcgc aagggaaccg ccatcacctt cgtcggcgag
      421 tgggacctgg acgcctggga gcagatccgc gagcaggtcg gtgccgagat cgaggaaggc
      481 cagctcgacg tctacggcgc cggatgaacg acggggcgct cccggcggta aggtaagttg
      541 ccgatggcga aggaactgac cctgcggccg cagctggcga gcccgtcgga cgcggtcatt
      601 cagagcgtcg agtggctgtt cgagcccgcc tggcgcggcg acaggctcat cgcgcgcatg
      661 cgcgccggac gcgtcagcct gaccgatgag gacggccagc ccaccagccg ccccgacctg
      721 gacgaggtgg tcgaggtcct ggagcccgcg ctgcgcaccg aggacgcggt gatcgacggc
      781 atctggaccg ccatgccctt cgtcggcgag gggagcgccg cacggcacct ggccgatgcc
      841 atcgaggagg aggggctgac gggcgaagtg cccgatccca tcgagaacga gccgcggcgc
      901 gccttcgtgg ccatcgacct ggtggagatc gacggccagc tgctgcacga cgtgccctac
      961 caggagcgtc gtcggctgct gaccgccatc gtcgacgagg acatccgggt acgggtcacc
     1021 cccgccgtcc gcgtcccgct ccacaactgg ctgctggcct ggcgggccgc cgggttcagc
     1081 cactacgtgg ccaagcacat gaactcgcgc taccgccccg gcgaggtggc cgaggactgg
     1141 ctgctgatct ccaccgccga gcgccgcggg gcaaatccga tcggcaggct gttcggcatg
     1201 cggcccaaga aggtgctgca catcgaggac gacggcaacc caaaggtccg ccgctgacct
     1261 gtcgctgccg ccccgtccag gtgccgatgt gtcgtaggcg ccaccccatg ggtgcctcgg
     1321 ccaggcgcgg ccacgcaacg gccactacgg ccaagaagcg cccgatgaac cgctgcggat
     1381 ggccgtgacg ctcacctggt ggccacggcg gccaagatgg gcgggattat ggtgtctgac
     1441 tggccgttgg agccgctggg tggccgcgcg cagcgcacgc cactccatcg gtccaggagg
     1501 tgcaccaacg gcggtccgtg ctacgcttcc aatcccgagc gagcaccgcc aagcgaccac
     1561 gcgagcacgc ccaggaggtc ccgccgctgc cgtccaaccc cacgaccacc cccgattcgg
     1621 cgctcgacga gaagggcctc gaggaggtcc gccgcgtcgg cagtgcggac ctgatggtcg
     1681 gcatcccgtc cttcggcaac gccgagacga tcggctacgt ggtgcgtgcc gcccaggccg
     1741 gcatggtgca gttcttcccg gatctcaagc cggtgctggt caacagcgac ggcggctccc
     1801 cagacgacac gccgcgggtg gcggtcagca ccgagagccc ggagtacatc gagaagatca
     1861 tcctggtctc gtcccgccac aagctgcagc gggtgacctt cacctaccag ggcaccagcg
     1921 gcaagggcac cgcggtgcgc gccctgttcg agggggcgcg tgagctgaag
//
LOCUS       DATACA010000153         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1869110, whole genome shotgun sequence.
ACCESSION   DATACA010000153 DATACA010000000
VERSION     DATACA010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869110"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1154
                     /locus_tag="VHK28_03120"
     CDS             120..1154
                     /locus_tag="VHK28_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2141257.1"
                     /translation="MIPPGRDPIADLRRIAFLLEAANEPSYRVRAFRNAAAAIGGLEP
                     EELAERARSARLRELPGIGEVLERTISESLRGEEPVYLRRLEATGGRMVAELGAEICS
                     ALRGDCHVHSDWSDGGAPIREMAEAAIEMGREYMVLSDHSRNLTVANGLSAERLERQL
                     KEVAVLNGELAPFRILTGIEVDILADGSLDQEPELLERLDVVVGSVHSELRMEARLMT
                     RRIVAALADPLLDILGHCTGRMKTGRRSRPPSTFDAEIVFAAAARFDKAIEVNSRPER
                     LDPPKRLLRLAVEAGCRVAVDSDAHAPGQLAWLPYGCDRAAACGVPLSAVVNAMTADD
                     LLAWTGSHES"
     gene            1239..1442
                     /locus_tag="VHK28_03125"
     CDS             1239..1442
                     /locus_tag="VHK28_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018774263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX2141258.1"
                     /translation="MSVARVTELSCTSNESFEDAIRQGIERAQRTLRQVRSAWIKEQR
                     VNIGSNGAVEFQVNILVTFVLED"
BASE COUNT          239 a          567 c          594 g          228 t
ORIGIN      
        1 ggcccggggt tcggctacgt ggcgcagcac ttcctgccca cgctgcgcac ggcggcggtg
       61 ggcgagggcg agatccacca gatgacggtc gagaacccgc gccgcctgct caccatcccg
      121 tgatcccgcc cggtcgggac ccgatcgccg acctgcggcg catcgcgttc ctgctcgagg
      181 cggccaacga gccgtcgtac cgggtccgcg ccttccgcaa cgcggccgcc gccatcggtg
      241 ggctcgaacc ggaggagctc gccgaacggg cacggtcagc gcgcctgcgg gagctgccgg
      301 ggatcggcga ggttctggag cgcaccatca gcgagtcgct gcggggcgag gagccggtct
      361 acctgcgacg cctggaggcg accggtgggc ggatggtggc cgagctgggg gcggagatct
      421 gctccgcgct gcgcggcgac tgccacgtgc actccgactg gagcgacggg ggcgcgccca
      481 tccgcgagat ggcggaggcg gccatcgaga tggggcgcga gtacatggtc ctctccgacc
      541 actcgcggaa cctgacggtg gccaacggcc tctccgccga gcggctggaa cgccagctga
      601 aggaggtggc ggtcctgaac ggggagctgg cccccttccg catcctgacc ggcatcgagg
      661 tcgacatcct ggccgacggc agcctggacc aggagccgga gctgctggag cggctggacg
      721 tggtggtggg cagcgtgcac tccgagctgc gaatggaggc gcgcctcatg acacggcgga
      781 tagtggccgc cctcgccgac ccgctgctcg acatcctggg ccactgcacc gggcggatga
      841 agaccgggcg tcgttcgcgc ccgccgtcga ccttcgacgc cgagatcgtg ttcgctgcgg
      901 ccgcccgctt cgacaaggcg atcgaggtca actcccggcc ggagcgcctc gacccgccta
      961 agcggctgct gcgcctggcc gtggaagccg gctgccgggt ggcggtcgac tccgacgcgc
     1021 acgccccggg ccagctcgcc tggctgccct acggctgcga ccgggccgcc gcctgcggcg
     1081 tgccgctgtc tgcggtggtc aatgcgatga cggccgatga cctgctggcc tggacgggct
     1141 cgcacgagtc gtagtccatc cgaatcggga catggcgcga gttgccggcg agaatcatcg
     1201 gtccatactt ggcggtccat cacgacagga ggagaccgat gtccgtcgcg cgcgtcaccg
     1261 agctcagctg cacgtccaac gagtccttcg aggacgccat ccgccaggga atcgagcggg
     1321 ctcagcgcac cctgcgccag gtgcgcagcg cctggatcaa ggagcagcgg gtgaacatcg
     1381 gctccaacgg tgccgtggag ttccaggtca acatcctggt caccttcgtc ctcgaggact
     1441 gagggcagcc actcctacca ggggggagcc agggcctcca tggtgtggcg gtcggccatc
     1501 aggccttccg cggtgagcgg gatgacggcc acgtggtcgg ccccggcatc cagcatggcg
     1561 cgcagccgag cctggatggc cgttacgtcg ccccaggcca ccaggtcgtc gaccacccgg
     1621 tcggcacc
//
LOCUS       DATACA010000154         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_423166, whole genome shotgun sequence.
ACCESSION   DATACA010000154 DATACA010000000
VERSION     DATACA010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423166"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VHK28_03130"
     CDS             complement(<1..615)
                     /locus_tag="VHK28_03130"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEX2141259.1"
                     /translation="MVVVASGEPHPDDAVWLQDEALVIAADGGAGWLETLGRTPDLLV
                     GDLDSIDPRLLADLERTGVAVERHPADKEASDAELALLRAAEAGDEVVLIGALVGLRL
                     DHELANLLLIADRRWRDALDDLRIVRGRTLVRALHGGESLAVEAAPGSTVTLLPIGGD
                     AEGVRTAGLRYPLDGDALRLGRSRGLSNQVAQAPASVSLARGTLL"
     regulatory      complement(718..821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            864..1349
                     /locus_tag="VHK28_03135"
     CDS             864..1349
                     /locus_tag="VHK28_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2141260.1"
                     /translation="MPDAPHPNVERVMRAAADAGLSISVQRFGVETRTASDAARAVGC
                     EVGQIVKSLVFIADGAGVVALLSGAHRLDTDRMAAHLGAADVRRATGDEARQATGYAI
                     GGVPPIGHTNRLPVLMDEGLLEHDVVWAAAGLPDAVFSAEPRALASAAGASIATLAER
                     G"
     gene            complement(1362..1850)
                     /locus_tag="VHK28_03140"
     CDS             complement(1362..1850)
                     /locus_tag="VHK28_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141261.1"
                     /translation="MDPGSVTSPGTPRRAGWAAARLAVAMVALAACSPSPDSPSPSAG
                     LLSDSPRPDASEAVSSSAEPSARVPGELPAGFPVPPGAESRTIQPGERIIARWQLNDH
                     GSAAYDYFLRELPAAGFTVEAVAPGGAAASIRITTPHGEKLQIDLVGTQPVQVELGPP
                     HE"
     gene            complement(1911..>2796)
                     /locus_tag="VHK28_03145"
     CDS             complement(1911..>2796)
                     /locus_tag="VHK28_03145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2141262.1"
                     /translation="PNVGLSYGMDRDWRLGVQLDWAGKVWWDSVPHRGRVYDNHTGLD
                     YPMALRSTIAAARAGTVIDVEGGFGTQQKGQFGNFVLMSHSDGRRTLYYHLASAAEGG
                     IHHGVGKQLAAGASVGLSGCSGICSGPHLHFEVLLVSGQQEVPTDPMAHRMWTTWPGR
                     VPFLAAYVRENNAGTEVVRRGQTITHWVEFRNTGGRTWRPDIFPGRVVLGTWNPPAHA
                     SPFRAADWPLSWMATKVDQASVPPNSVGRFTFGIRGGPAVGSYVETFNLVAQSVRWFD
                     HARLGGFYVPIVVSNFSP"
BASE COUNT          395 a          996 c         1006 g          399 t
ORIGIN      
        1 caggagcgtc ccccgcgcga gcgagaccga tgcgggcgcc tgcgccacct ggttggagag
       61 ccctcgcgac ctacccagcc gcagcgcatc accgtccagc ggatagcgca ggcccgcggt
      121 ccgcacgcct tcggcgtcgc cgccgatcgg cagcagcgtg acggtcgagc cgggtgccgc
      181 ctcgacggcc aaactctccc cgccgtggag ggcccgcacc agcgtcctgc cgcgcacgat
      241 ccgcaggtca tccagcgcat cgcgccaccg ccggtcggcg atcagcagca ggttggccag
      301 ctcgtggtcg aggcggagac cgaccagcgc accgatgagc acgacctcgt cgccggcctc
      361 ggccgcacgc agcagcgcca gctcggcgtc cgacgcctcc ttgtccgccg ggtggcgctc
      421 caccgctacc cccgtccgct cgaggtcggc gagcagtcgc gggtcgatag agtcgaggtc
      481 acccaccagc aggtcgggcg tccgccccaa ggtctccagc cagccggcgc cgccgtcggc
      541 ggcgatgacc aaagcctcgt cctgcagcca aacggcatcg tcggggtgcg gctcgccgct
      601 ggcgaccacg accactcgca tccaacctcc cgtgcgtgca ccgcgggtgt tgcggtgctc
      661 gtcgcttcat cggtcccgcg agacgacgaa cggcgcaccc tggagggcgc gccgctggca
      721 ctgttcaaac cgactccctt cgctggcatg atccagatca ggttcgaggg tctgcggctg
      781 gccgcactct cagcgcacgt ggcgctcccc tgtcgctctt gggttgtcgt ccccgagcct
      841 agcagcatgc gatcatcggc gccatgcccg atgcacctca tccgaacgtc gagcgcgtga
      901 tgcgcgccgc ggcggatgcc ggcctgtcga tcagcgtcca gcgcttcggc gtcgagacgc
      961 ggacggcgtc cgatgctgcg cgcgccgtcg gctgcgaggt ggggcagatc gtgaagtcgc
     1021 tcgtcttcat tgccgatggg gcaggcgtgg tcgccctgct gtccggcgcg catcggctgg
     1081 acacggatcg gatggcggcg catctgggcg ccgccgacgt gcggcgcgcc accggtgacg
     1141 aagcgcggca ggccacaggc tacgccatcg gcggggtccc gcccatcggg catacgaatc
     1201 gcctgccggt gctcatggac gaggggcttc tggagcacga tgtcgtgtgg gccgcggccg
     1261 gcctgccgga tgctgtcttc agcgcggagc cgcgggcgct ggcaagcgcg gccggggcat
     1321 ccatcgccac gctggcggag cgcggctgag caagtgcgcc gtcactcgtg tggcgggccg
     1381 agctcgacct ggaccggctg cgtcccgacc aggtcgatct gcagcttctc gccgtggggg
     1441 gtggtgatcc tgatgctggc agccgcccct ccgggagcta ctgcctcgac ggtgaatccg
     1501 gccgccggca gctcgcgcag gaagtagtcg taggcggcgc tgccatggtc gttcaactgc
     1561 cagcgggcga tgatccgctc tcccggctga atcgtgcggg actccgcgcc gggcggcacg
     1621 gggaagccgg cgggcagctc gccgggcacc cgagcggagg gttctgccga ggacgagacc
     1681 gcctcgctgg catccggccg agggctgtcg gacagcaggc cggccgaggg cgagggcgag
     1741 tcgggagagg ggctgcaagc ggcaagggcg accatcgcca ccgccagccg ggctgctgcc
     1801 cagccggctc ggcgcggggt cccggggctg gtgacggacc ctggatccat gtgcacctcc
     1861 gtggtggtgg tggtgcgggg gcgtcccggc gaacgccccc cgcgagtcgt tcacgggctg
     1921 aagttgctga ccacgatcgg cacgtagaac ccgcccaggc gggcgtggtc aaaccagcgc
     1981 accgattggg cgaccaggtt gaacgtctcg acatagctcc cgaccgccgg gccgccgcgg
     2041 atgccgaacg tgaagcgacc gaccgagttg ggcgggaccg atgcctggtc gaccttggtc
     2101 gccatccagc tcagcggcca gtcggcggca cggaagggag aagcgtgagc cggcgggttc
     2161 cacgtcccga gcaccacacg accggggaag atgtccgggc gccaggtgcg accgccggtg
     2221 ttgcggaact cgacccagtg ggtgatcgtc tgccctcggc gcacgacctc ggtgccggcg
     2281 ttgttctcgc gcacataggc ggccaggaaa ggcacgcgcc ccggccaggt ggtccacatg
     2341 cggtgggcca tgggatcggt gggcacttcc tgctggccgc tgaccagcag cacctcgaag
     2401 tggaggtggg gaccgctgca gatgccgctg caccctgaca gtcccacgct ggcaccggcc
     2461 gccagctgct tcccgacgcc gtggtggatg ccaccctcgg cggcgctggc caggtggtag
     2521 tagagggtcc gccgcccgtc ggaatggctc atcagcacga agttgccgaa ctggcccttc
     2581 tgctgcgtgc cgaacccgcc ctccacgtcg atcaccgtgc ccgccctggc ggcggcgatg
     2641 gtcgagcgca gggccatcgg gtagtccagg ccagtgtggt tgtcgtacac ccgtccccgg
     2701 tgcgggaccg agtcccacca caccttgccg gcccagtcca gctgcacccc gagacgccag
     2761 tcgcggtcca tgccgtagct gagaccgacg ttcggg
//
LOCUS       DATACA010000155         2897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_905621, whole genome shotgun sequence.
ACCESSION   DATACA010000155 DATACA010000000
VERSION     DATACA010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905621"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VHK28_03150"
     CDS             complement(<1..842)
                     /locus_tag="VHK28_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX2141263.1"
                     /translation="MRRPAVCIYDVVDPENRLVAAELERRWEQALQAAAAAREAAERV
                     ARANRADAARVAFPDADSFAPVRVRVGVQEGVVVRRGEERRRLEIKATAEAELAPPGA
                     DGLAPFAAGGGYDGPLAYRQGIPMRPDVALAFDRMARAARTDGIALSIGSGYRSDAEQ
                     AELFARNPDPKWVAPPGTSLHRYATELDLGPPSAYGWLAANAPRFHFVLRYPHEPWHF
                     GFTLNARSTRRGPGDGARRTGLPAFVPARFAPILTRAAQRWNVSATMLAAQLYAESNF
                     NPFAV"
     gene            853..2439
                     /locus_tag="VHK28_03155"
     CDS             853..2439
                     /locus_tag="VHK28_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141264.1"
                     /translation="MLDDDLVVGHHDLLHHQPQDLLLHREGWLNQLRLEARAEGTDRL
                     RQTRRLVRLNELLADRLRPLVEAVPRRAQPLAAHRQLVQLQHPGLIGVDEALVLSPQC
                     RLLPLNTLQFLLGIAQGWAIPRLLLAQVGHDELWLAEQGPDMLPDHRLDIALADGPQV
                     AAPLRRLAAAPPRAFVAPAGHPGVPAQAPPTGAADEQAGQEILVARLARRALAIELQL
                     PLGHSEGLHVNDGRPGDTDPLGGGAQAAPRVFGRPPVPLARFLRRILRPIIVVLAGVE
                     AVGEDLVDSGGRPGHAPGAWRDALLGQAPSDGSHRQLVFDEPGEDLAHRRRLGLVDRA
                     GAALAVAGQIGVAVGTGPGEEAAGTGGVQLATAATLLEGGPLEFSEQAMHLAHQALFG
                     AGGRGTGDEDDRAAGPFQFLNNDVLVGIVAGQPVGGQDEHLREFPAEGGVTQPVEAGA
                     IEAGTAGAIVDVAMLGRDRQTMRGGVGVQGGELAGNGALLLLLGRRDTGIEGGGRHGA
                     TSDDGEDGAAEGSAATIVLG"
BASE COUNT          413 a         1005 c         1045 g          434 t
ORIGIN      
        1 accgcgaacg ggttgaagtt gctctcggcg tagagctgag ccgccagcat ggtggccgag
       61 acgttccagc gttgcgcggc gcgggtcagg atcggcgcga agcgcgctgg cacgaacgcg
      121 ggcaggccgg tgcgacgcgc gccgtcaccc gggccgcggc gcgtcgagcg cgcgttcaac
      181 gtgaagccga agtgccacgg ttcgtgcgga tatcggagca cgaagtggaa gcgcggcgcg
      241 ttcgccgcca gccagccgta ggccgatggc gggccgaggt cgagctcggt ggcgtagcgg
      301 tgcagcgacg tgccgggcgg cgcgacccac ttcgggtccg ggttgcgggc gaacagctcg
      361 gcctgctcgg cgtccgaccg gtagccgctg ccgatcgaga gggcgatccc gtcggttcgc
      421 gccgcgcgcg ccatgcggtc gaacgcgagc gcgacgtcgg gccgcatcgg gatcccctgc
      481 cggtacgcga gcgggccgtc gtacccgccg cccgccgcga acggcgccaa cccgtccgcc
      541 cccggcggcg cgagctcggc ctccgccgtg gccttgatct cgagtcttcg gcgctcctcc
      601 ccacgccgca ccaccacgcc ctcctggacg ccgacgcgaa cccgcaccgg agcaaagctg
      661 tcggcgtcgg ggaacgcgac ccgcgccgcg tccgcgcggt tggcgcgcgc cacccgctcg
      721 gccgcctcgc gcgctgcagc cgctgcctgg agtgcctgct cccagcgccg ctccagctcg
      781 gccgccacca ggcggttctc ggggtcgact acgtcgtaga tgcaaacagc tggtcgacgc
      841 acctgggatg atgtgctcga tgacgatctg gtcgtcggtc accacgatct gctccaccac
      901 cagccgcagg atcttctgct tcaccgcgaa ggctggctca accaactgcg cctggaggcg
      961 cgtgcagaag gtactgaccg tctccgccag actcgtcgcc tggtgcgcct gaacgagttg
     1021 ctggcggacc gcctgcgccc gctcgtcgag gcggtgcccc gccgcgcgca gccgctggcg
     1081 gcgcaccgtc aactcgtcca actccagcac cccggcctga taggcgtcga tgaggcgctg
     1141 gtcctctcgc cccaatgccg cttgctgccg ctcaacacgc ttcagttcct gctgggcatc
     1201 gctcagggct gggccatccc ccgcctgctg ctcgcgcagg taggccatga tgagctctgg
     1261 ctggctgagc agggtccgga tatgctccca gaccaccggc tcgatatcgc gctggcagat
     1321 ggcccgcagg tggcagcgcc gctccggcgg ctcgctgctg cgccgccgcg tgcattcgta
     1381 gcgcccgctg gccaccccgg cgtgcccgcc caggcgccgc ccacaggcgc cgcagacgag
     1441 caggctggac aagagatact cgtggcgcgt cttgcgcggc gagcgctcgc gattgagctg
     1501 cagttgccgc tcggccatag cgaaggtctc cacgtcaatg atggccggcc aggggatacg
     1561 gatccactcg gtggcggggc gcaggcggcg ccgcgggtct ttggtcggcc gccagtaccg
     1621 ctggctcggt tcctccggcg cattctccgt cctattatag tagtgctcgc cggtgtagag
     1681 gcggttggtg aggatcttgt ggacagtggc gggcgaccag gccacgcccc cggcgcgtgg
     1741 cgggatgccc tgctcggtca ggcgccgagc gatggcagcc accgacagct ggtcttcgat
     1801 gagccaggcg aagatctggc gcaccgtcgc cgcctcggtc tcgttgaccg cgcaggtgcc
     1861 gccctggccg tcgctgggca gataggtgta gccgtaggca ccggccccgg tgaagaagcg
     1921 gccggcacgg gcggcgtgca gcttgccacg gcggccacgc tcctggaagg cggcccgctc
     1981 gaattcagcg aacaagccat gcacctcgcg caccaggcgc tcttcggggc tggtggccgt
     2041 gggaccggtg acgaagacga ccgtgcagcc ggcccgttcc agttcctcaa taacgacgta
     2101 ctggtaggca tagtggcggg ccagccggtc gggggccagg atgagcacct gagagagttc
     2161 cccgcggaag gcggcgtcac gcagccggtc gaggccgggg cgatcgaggc gggcaccgct
     2221 ggagccatcg tcgacgtagc gatgctcggg cgcgatcgtc agaccatgcg cggcggcgta
     2281 ggcgtccagg gcggcgagct ggctggcaat ggtgccctgc ttctcctgct gggccgtcga
     2341 gacacgggca tagagggcgg tggtcgtcat ggtgcaacct ccgatgacgg tgaggatggt
     2401 gcggccgagg gcagcgctgc gacgatcgtg ctcggctgat cggtgggact cgcccgcgcc
     2461 agcagactga aggccagact gaggcgctgg gcggcatcgc ggcgcgcgac acaggtgagc
     2521 accacgtggc ggggcggacg gacagccgga cggggtgggc gttcggcacg ctgggcagcc
     2581 ataccacctc ctctcggcga ctggcactga ccgatggccg gctcccgcag tccaaccagt
     2641 cgctgatggc agtatggccc acgctggcca tgccagcggc gtgcttcgcc gcccacctca
     2701 cacctcctca tggaccgacc agcggcggtc acggcgctga taccgcgcgg tggcttcccg
     2761 ccgtaagggc acggtggtgt agaaggtcag atgggcgttt gcatctacga cgtaaccgcc
     2821 ataaagcggc ggcgggcgag gtcggcttcg tagtgtgcac gttccagctg cagctgccat
     2881 tggtgctcca cctcgcg
//
LOCUS       DATACA010000156         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1146307, whole genome shotgun sequence.
ACCESSION   DATACA010000156 DATACA010000000
VERSION     DATACA010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146307"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VHK28_03160"
     CDS             complement(<1..1345)
                     /locus_tag="VHK28_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HEX2141265.1"
                     /translation="MARPRPRIAVALASLLVLAACSAGSSQTVTGERPQPPLTDPPPG
                     VGGVTNPNDEPYPDVFFDNPGVNPFVDTEDDALSTFALEVDTGSYTVARRFLTDGNLP
                     DKDAIRVEEFVNYFPTGVPAPDGEAFAIHADAAVTPFVQNDRYQVARVVIRSREMAPE
                     TRPDAALTFVIDVSGSMEMENRLELVKQSLRLLVTQLRLSDTVAIVVYGSDARVVLQP
                     TPLSEVETVQAAIDALHPEGATNAEAGLVLGYQLARAAFRPGGVNRVILASDGVANVG
                     NTGPDSILAQIRGEAAGGIQLATLGFGMGNYNDVLMEQLADDGDGFYAYVDTIDEAER
                     LFVDELTTTLVTVALDAKVQVSWDQAAVERWRLIGFENRAVADDQFRDDRVEAGAIGP
                     GHEVTALYELKLTDEAASTTSLGTVGLRWTDPDSGEAVEMQRQLAGLVTPAFPNAD"
     gene            complement(1804..>2736)
                     /locus_tag="VHK28_03165"
     CDS             complement(1804..>2736)
                     /locus_tag="VHK28_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX2141266.1"
                     /translation="SVHTGLNSLTLLQWGNEDQKERYLIPQARGEKLATFGLTEPGVG
                     SDAANLSTTARRDGDRYILNGSKIWISLADTADHFLVFATVDRSAGHRGITAFIVERG
                     FSGFSSDSLHGKLGVRAGNTGILYFNDCEVPAANRVGEEGEGFTIAMSAIDQGRYTVA
                     AGSVGLAQACLDASVKYAHERKAFGEEIGRHQLVKQMIARMYQGIEAGRLLVWKAGWK
                     KNAGERNTRETSLAKWFCTDHAVASALDAIQVHGAYGYSNEYPVERYLRNSKGSVIYE
                     GTSQIHTLMQADYLLGYRRDRDLRMPQPAFEGEM"
BASE COUNT          410 a          942 c          918 g          466 t
ORIGIN      
        1 gatcagcgtt ggggaacgcc ggtgtcacca ggccggccag ctggcgctgc atctcgaccg
       61 cctccccgct gtccggatca gtccagcgca ggccaaccgt tcccagggac gtggtgctcg
      121 ccgcctcatc ggtcagcttg agctcgtaca gcgccgtcac ctcgtgaccg ggcccgatgg
      181 ctccggcctc gacgcggtcg tcgcggaact ggtcgtccgc cacggcccgg ttctcgaagc
      241 cgatgaggcg ccagcgctcg accgcggcct ggtcccagct gacctgcacc ttggcgtcca
      301 gcgcgacggt caccagtgtg gtggtcagct cgtcgacgaa cagccgctcg gcctcgtcga
      361 tggtgtcgac gtaggcgtag aagccgtccc cgtcatcggc cagctgctcc atcagcacgt
      421 cgttgtagtt gcccatcccg aagcccagcg tggccagctg gatgccgccg gccgcctcgc
      481 cgcggatctg cgccaggatc gagtcggggc cggtgttgcc cacgttggcc acgccatcgg
      541 aggccaggat cacgcggttc acgccgcccg gcctgaacgc ggcacgcgcc agctgatagc
      601 cgagcaccag cccggcctcg gcgttggtgg ctccctctgg gtggagggca tcgatcgcag
      661 cctggaccgt ctccacctcg ctcagcggcg tgggctggag cacgacgcgg gcatcggacc
      721 cgtagaccac gatggccacc gtgtcgctca gccgcagctg ggtcaccagg agccgcagcg
      781 actgcttcac cagctccagg cggttctcca tctccatgga tccggagacg tcgatgacga
      841 aggtcagcgc ggcgtccggc cgcgtctcgg gcgccatctc gcggctgcgg atgaccaccc
      901 gcgccacctg gtagcggtcg ttctgcacga acggggtcac cgcggcgtcg gcgtggatgg
      961 cgaaagcctc gccatcgggc gccggcaccc cggtcgggaa gtagttcacg aactcctcga
     1021 cgcggatggc gtccttgtcc gggaggttgc catcggtcag gaaccgacgg gcgaccgtat
     1081 aggacccggt gtcgacctcc agcgcgaagg tcgatagcgc gtcatcctcg gtgtccacga
     1141 aggggttcac gccggggttg tcgaagaaga cgtccgggta cggctcgtcg ttcggattgg
     1201 tcacgccgcc gacgcccgga ggcggatcgg tcagcggcgg ttgcggccgc tcgccggtca
     1261 ccgtctgtga gctcccggcg ctgcatgcgg ccagaacgag cagggacgcc agggccacgg
     1321 cgatccgtgg gcgtggtcgt gccatggttg ccacctcgct tttccctcag ccatgtgatg
     1381 gtctgtgcga tcgcgattgg tggccggcca gcagtatcta ccgattccgt ggcagcttcg
     1441 ttcagtcgct cccccatccc cgccctgacc caaccgcgcg gacctcaccg aaggcgccaa
     1501 caccctcctt tggaccgatg cggattcggt ggtcttcccc gcctgtcacg acgcaaaccg
     1561 accggtacgc ggccggtaag ggcaagtcct gcaccacgtc tgcatggacg atcgccagct
     1621 cgggataacc attcgactcc tgcgccagcg atgcggatgg cgccaggtcg acctcgcgat
     1681 tcgcgccggt caggcgagcg tgcagaaggg cgccccggcg gccgtgcagg ccagtcaacg
     1741 gcctaagcgc agccaggctg cgatctggag attggtggca gggcgctggc aggaactgcc
     1801 cttctacatc tccccttcga aggcaggctg cggcatgcgc aggtcccggt cgcggcgata
     1861 tccgagcagg taatcggcct gcatcagggt gtggatctgg ctggttccct cgtagatgac
     1921 cgagcccttc gagttgcgga ggtagcgctc caccgggtat tcattcgagt agccgtacgc
     1981 gccgtggacc tggatggcgt ccagcgccga ggccaccgca tgatcggtgc agaaccactt
     2041 ggccagcgat gtctcgcgcg tgttgcgctc ccccgcgttc ttcttccagc cggccttcca
     2101 gaccaggagg cggccggcct cgatgccctg gtacatcctg gcgatcatct gcttgacgag
     2161 ctggtggcgg ccgatctcct cgccgaacgc cttgcgctca tgcgcgtact tgaccgatgc
     2221 gtccaggcag gcctgcgcaa gcccgaccga accggcagcg acggtgtagc gcccctggtc
     2281 gatggcgctc atggcgatcg tgaagccctc cccctcctca ccaacgcggt tggccgccgg
     2341 tacctcgcaa tcgttgaagt acaggatccc ggtgttgccg gcgcgaacgc ccagcttgcc
     2401 gtgcagcgag tcactgctga acccggagaa tccgcgctcc acgatgaagg cggtgatgcc
     2461 gcgatgtccg gccgaccggt ccaccgtcgc gaagaccagg aagtggtcgg cggtatcggc
     2521 caggctgatc cagatcttgg agccgttgag gatgtatcgg tcgccgtcac ggcgggccgt
     2581 ggtggacagg ttggccgcgt cggagcccac gccgggctcg gtcaggccga aggtggccag
     2641 cttctcgccg cgcgcctgcg ggatcaggta gcgctccttc tggtcctcgt tgccccactg
     2701 cagcagggtc agcgagttca ggccggtatg gaccga
//
LOCUS       DATACA010000157        13035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1949608, whole genome shotgun sequence.
ACCESSION   DATACA010000157 DATACA010000000
VERSION     DATACA010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13035
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949608"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..817)
                     /locus_tag="VHK28_03170"
     CDS             complement(71..817)
                     /locus_tag="VHK28_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141267.1"
                     /translation="MTVILEARDLVKRYPLRGSELAALRGVSLSVTQGEFVAIMGTSG
                     SGKSTMLHLLGGLDRPTSGEVVIDGVNISQLDDEQATLTRRAKTGFVFQFFNLIPLLN
                     VAENVALPFLIAGDAVTDHRERVNDLLELVGLADKAAHRPDQLSAGEQQRVALARALA
                     TAPAILLADEPTGNLDYTTGSEILDLLWDSCDRLGQTIVLVTHDARAAAYADRVLVVR
                     DGSIVEEIELGRRKDREARPLIARLAALGL"
     gene            complement(881..1741)
                     /locus_tag="VHK28_03175"
     CDS             complement(881..1741)
                     /locus_tag="VHK28_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX2141268.1"
                     /translation="MAAALLAWTPQPATGWNQGAAEGTVWQLLNGARVNNGRRAVQQH
                     STLVSLARWRSKDQVDRNYFDHTILGSGCQVYCWYDSNGLNYTWGGENIGWNNGFSDT
                     DSPVKIHEGFMNSPGHRANLLEPSFTHGGVGAYARDNVNFLGKLRSPRFYTQLFMTAA
                     SAPPPPPPPQPSGNTPPPPPRSGGGGGGGSRPAATASAAPNAAEVRTERSAPRPSQPL
                     DGAESLVAAQPSVSTAAVRALPWTDGAEGGSPSARPALVASRLRVEAAVTPDRGMFET
                     VIGSVLGVLF"
     gene            1979..2374
                     /locus_tag="VHK28_03180"
     CDS             1979..2374
                     /locus_tag="VHK28_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX2141269.1"
                     /translation="MTDASSPAPTIGRTRWAIAEGYIPSQSTGPEPAMTSHETACLLN
                     AGGQDAQVSIWIYFADRDPAGPYTVRVPARRTLHLRFNELQDPEPLPRDTDYASVIQS
                     DVPIVVQHTRLDSRQAENAILSTIAYAGE"
     gene            2371..2934
                     /locus_tag="VHK28_03185"
     CDS             2371..2934
                     /locus_tag="VHK28_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX2141270.1"
                     /translation="MTDTRPCRVVLVGMMGSGKTTIGRALAEHTGWGYHDNDELLAAA
                     TGRSAAEIALSGEAALRSAEAAALAEALSMPPPTIVAVAAGSVLDRDHRERLRRDALV
                     VWLHAAPERLASRATGAAHRPWLDDDAAQWFARAAAEREPLYRAVADLVVETGASGPA
                     AAAERILDWLRGTACARWVPPAREAQP"
     gene            2965..3420
                     /locus_tag="VHK28_03190"
     CDS             2965..3420
                     /locus_tag="VHK28_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX2141271.1"
                     /translation="MDDRNPRRDQPLPSEDTGDLAETPPSGETDDFLVAREEGVPYVP
                     PTDRVLSQSRQGESGPDQAAAAPDDASELSRQAPITDDQQDLTARAAEALRMSDVTAG
                     DRLRVGSVGSTVYLRGQVESVDIADELTSILGDVAGVDEVVDETQVRGL"
     gene            3575..3964
                     /locus_tag="VHK28_03195"
     CDS             3575..3964
                     /locus_tag="VHK28_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141272.1"
                     /translation="MSTGAERESGVQRPNAPDAGWPTGMPYTVHFPYEGDYLGADLEH
                     PAPLPRVGDVVEYFDERGACHRFRVREVIHTLQTSAAYRPAVADGTASPQSVARLDED
                     VEAEPPGGSGVVRAGLPKVILETVREE"
     gene            4136..5092
                     /locus_tag="VHK28_03200"
     CDS             4136..5092
                     /locus_tag="VHK28_03200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006108782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2141273.1"
                     /translation="MTTIGYALSSEEHPPRNLVGHAAAAERAGFRFALISDHFHPWTS
                     RQGHSPFVWSVLGGIAHNTSSLEVGTGVTCPLIRTHPAIIAQAAATSASMFEGRFFLG
                     VGTGENLNEHIFGDAWPRYATRNEMLREAVEIMRELWTGELTSYEGAYYRVENAQLYT
                     LPERPPAVMVAASGPESAQLAAEIGDGMITTTPDKELVDAFRQSGGEGKPLYGQLTVC
                     WAPTRDQALDVAMEWWPTAGVPGDLSQELPLPMHFEQVSKLVTRDQLAQSVLCGPDAD
                     EHLQRIGQFTEAGFDHVYVHQVGPDQEGFFRFYADQVLPRLT"
     gene            5140..5733
                     /locus_tag="VHK28_03205"
     CDS             5140..5733
                     /locus_tag="VHK28_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141274.1"
                     /translation="MAHPPLVHVKTCNRCGRTGSHAFRGTPDGLYECTTVTACRARTR
                     RNAGLRGEGRGRLPKQAGLNGAPPGVAYVIGQADAPRALVEETLRDATPLSVAVGEPN
                     RATLTALGSRNVKLIAIDARCLARIGFRNEFSLRRRQPRLSSVPVFLYGDDATLAPMA
                     PQFPDARLIGFANGPREAIRRLRREVRLPEAAVRARA"
     gene            complement(5735..5974)
                     /locus_tag="VHK28_03210"
     CDS             complement(5735..5974)
                     /locus_tag="VHK28_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141275.1"
                     /translation="MSERERTNQPDAGAFINNRGEAAAETVPGGVQEDDERVAGHDRE
                     SSGTGAKEHRVQGRDDEWPQGHRETDVNPDDELMP"
     gene            6049..7701
                     /locus_tag="VHK28_03215"
     CDS             6049..7701
                     /locus_tag="VHK28_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX2141276.1"
                     /translation="MVEAHSRPAWYSQAVIYAIDVGSYRDSDGDGVGDLVGVSQALPY
                     LQRLGIDCIWLLPFFATENRDNGYDVTDYYAVDPQFGTLGDFGDLVDEAEQRGIRIVI
                     DLVVNHTSDRHPWFQAARSRDPSPYRDYYIWNDQPPPLREQPPSIFPDRVPSVWTWDE
                     QRGSFYYHQFYPFQPALNHLNPDVRDEVRRIIAFWLRLGVAGFRLDAASHLIDPPLDG
                     EPADRVEILGEYRRHVVKGRGDAILIGEADLPEKDLEPLMGEHALQLLFNFVLNGHAF
                     LAMARGSAEPIVEAIGRLPHPADAGWANFLRNLDEVDLERLTDAEREEVFAAFAPDEG
                     MRIFNRGIRRRLASMLDGDQRRLELAYAILLALPGAPVIVYGDEIGMGDDQSLPEREA
                     VRTAMQWTSGTRAGFTPADTNRRSVKVISGGRFGAERVNVADQENDPGSLLRWLEEAI
                     RVRRATPELVAGGAEVTALADGAVLARRCELDGVRVVALHNLAGHQVSLSRDELGLEA
                     GPVQRLLADGRSPDPTAGKRAVRLAPYGYLWLRMEWSDEEAG"
     gene            7698..9302
                     /locus_tag="VHK28_03220"
     CDS             7698..9302
                     /locus_tag="VHK28_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2141277.1"
                     /translation="MTDSHLWWQTGVIYEIYPRSFADSDGDGAGDLPGVRGRLDYLRW
                     LGVDAIWLTPFYPSPMVDGGYDVADYTDVHPMFGDLQQFDLLVAEAHRRSLRVIVDLV
                     PNHTSDQHPWFIESRSSRDNPRRDWYIWRDPAPDGGPPNNWISEFGGSGWTLDESTGQ
                     YYYHAFAEAQPDLNWRNPDVRRAMYDVMRFWLDRGVDGFRVDVMWHVIKDELLRDNPP
                     NPDYREGVDSPSRRLIQAYSTDQPEVHDVVAEMRGVLDEYDERVMIAEIYLPVDRLVD
                     YYGRGERAGAHLPFNFHLILTPWDAREIELVVDRYEGALPANAWPNWVLGNHDLPRVA
                     SRIGPKQARIAAMLLLSLRGTPTLYYGEELGTEETPVPPEDVQDVREINQPGRGLGRD
                     PCRTPMPWDGSPGAGFTTGRPWLPIGEDNRQRNVEALRSDPDSMLSLYRRLLELRRRE
                     PALTIGSYRPVPASGSVLAFEREQDGRRLLVALNLSDQPAGLELEPDGRRYRPLLSTL
                     PDAAGGEVGPAIELRADEGLILEPLE"
     gene            9344..10531
                     /gene="chrA"
                     /locus_tag="VHK28_03225"
     CDS             9344..10531
                     /gene="chrA"
                     /locus_tag="VHK28_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX2141278.1"
                     /translation="MDDPPAAAPGSWGSVLGVALQLGLTSFGGPIAHLGYFRREYVER
                     RRWLDERDFADLVALCQTLPGPASSQLGIAIGRLRAGAAGALAAWVGFTLPSAAIMVG
                     FALLAGSTDLADAGGVAGLKLAAVAVVGHAVLRMSATLAPNLAQRLLALVSAGVLLVL
                     EGPVMQVGVLAAAALLGYAWTHGQRRSPPTARVGGRRWGMVALVVFGVLLIALPVLRA
                     VDGQVVALVDTFYRAGSLVFGGGHVVLPLLDAGVVGPGWVSEDQFLAGYGLAQAIPGP
                     LFTFSAYLGAVAAPEPNEVLGAVLALVAIFLPSFLLVFGVLPFWERLRGSPRVRGALD
                     AVNVAVIGLLLAALYHPVATAAIGSPLDAAVALGGLALLSARVPPLAVAAGAAVAGQL
                     IGP"
     gene            10729..12858
                     /locus_tag="VHK28_03230"
     CDS             10729..12858
                     /locus_tag="VHK28_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011776931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2141279.1"
                     /translation="MPSDPHRAPLDAGASTSDAHAGHGGADGGGHGGGAHGGGAHGGH
                     DKHAGHDPEAFRRQFWLVLLLTIPVVVWSEEVQMWLGYRAPAFPGSEWVAPVLGTLVF
                     LYGGRVFLSGARTELGDRQPGMMTLISLAIVVAFVTSWAATLGLFEVEVWWELATLIT
                     IMSLGHWLEMRSIMQARGALSALAELLPDTAERVTPGGLETVPLDALQTGDVVLVRPG
                     GRVPADGKVAEGSADVDESMITGESRPVTRGPGDAVTAATVAAGGSLRVRVTAVGDET
                     ALSGIMRLVAAAQASSSRAQALADRAAAILFYVALVAGGLTLAAWSLLGDPEEGLIRT
                     ATVLVIACPHALGLAIPLVIAIGTTLGARNGLLIKDRLALERARELEVVVFDKTGTLT
                     RGRPVLAAAAPAAGVDEGELLEIAAAVEADSEHPLARAVVAAAQERGLRPPAVESFEA
                     LPGRGARARLRGVELRVGGPALLADAGAELPSEIASETEEWAAAGRTVLYVLRDGVVV
                     GAMAMADEIRPESVEAVRRLHEMGLRVAMITGDAQSVADDVARRIGIDELAAQVLPAD
                     KAAAVQRFQAGGQRVAMVGDGVNDAPALAQADVGIAIGAGTDVAVESAGIVLVRDDPR
                     DVPGAIELSRASYRKMIQNLAWATAYNAIAIPVAAGLFAPWGIVLAMSVGALVMSLST
                     IIVAANAQLLRGLRLRPEPSALAATPG"
BASE COUNT         1836 a         4539 c         4746 g         1914 t
ORIGIN      
        1 ccacggtcag ggcggtgcac agcgggcggg cggccaagcc acgcgaggcg agggcgccga
       61 gccgcacggg ctacagcccc agggcggcca ggcgggcgat cagcggccgc gcctctcggt
      121 ccttgcgccg ccccagctcg atctcctcga cgatggaccc gtcgcggacc accagcacgc
      181 ggtcggcgta cgccgccgcg cgggcatcgt gggtgaccag cacgatcgtc tggcccaggc
      241 gatcgcacga gtcccacagc aggtccagga tctcgctgcc ggtcgtgtag tccaggttgc
      301 cggtcggctc gtctgccagc aggatggccg gcgccgtggc cagcgcccgg gccagcgcga
      361 cccgctgctg ctccccggcc gaaagctggt ccggtcggtg ggcggccttg tcggccagcc
      421 ccaccagctc gagcaggtcg ttgacccgtt cgcggtggtc ggtgaccgcg tcgccggcga
      481 tcaggaacgg cagcgccacg ttctccgcca cgttcagcag cgggatcagg ttgaagaact
      541 ggaacacgaa gccggtcttg gcccggcggg tgagcgtcgc ctgctcgtca tccagctggc
      601 tgatgttgac gccgtcgatt accacctcgc cggaggtcgg gcggtcgagg ccgccaagca
      661 ggtggagcat ggttgacttg ccggaaccgg aggtgcccat gatggcgacg aactcgccct
      721 gggtgaccga gagcgagacg ccacgtagcg ccgcgagctc gctgccccgc agcggatagc
      781 gcttgacgag gtcgcgcgcc tcgagaatga cggtcatgct gccggcgagt ctagaccgat
      841 ggcgacctgc ggcgagcggc ggcctggcgt tccgccgccg ctagaagagg acgcccagga
      901 ccgagccgat caccgtctcg aacatgcccc ggtcaggggt gaccgctgcc tccacccgga
      961 gccggctggc gaccagggcc ggccgggcag agggagatcc gccctccgcg ccatcggtcc
     1021 agggcagggc tcgaaccgcc gcggtggata cgctcggctg ggcggccacg agtgactcgg
     1081 caccgtccag tggctgcgac ggtcgcggtg ccgaccgctc ggtccggacc tcggcggcat
     1141 tcggcgcggc gctggccgtg gccgccggcc gggagccgcc tccgcctccc ccgccggagc
     1201 gcggcggcgg aggtggcgtg ttgcccgacg gctgcggagg tggcggcggt ggaggtgccg
     1261 aagctgcggt catgaacagc tgggtgtaga agcgtggact gcgcagcttg ccgaggaagt
     1321 tgacgttgtc ccgcgcgtag gcgcccaccc cgccgtgtgt gaacgacggc tccaggaggt
     1381 ttgcccggtg ccccggggag ttcatgaacc cctcgtggat cttgaccggc gaatccgtgt
     1441 cggagaagcc gttgttccag ccgatgttct cgccgcccca tgtgtagttg aggccgttgg
     1501 agtcgtacca gcagtacacc tggcagccgc tgccaaggat ggtgtggtcg aagtagttgc
     1561 ggtccacctg gtccttgctg cgccatcggg ccaggctgac cagggtggag tgctgctgca
     1621 ccgcgcgccg gccgttgttc acccgggcgc cgttgaggag ctgccagacc gtgccctccg
     1681 ccgctccctg gttccagccg gtcgccggct gcggcgtcca ggccaggagc gcggcagcca
     1741 tcagcgcggt gccagccagg cccgcggccc tgagcaggac gcgggacggt cggcgatgaa
     1801 tcggggcgtg cgacaaggca agcaggctcc aggcagtccg taccgcacga gcctacgggg
     1861 caccgtcgcc ggggtcagta gtgctgtggt ccggtcgacg gggtgaccat gggcgtttgg
     1921 ggtaccggtt ggggatggga ccaacggctg gcacgtgcct tgcgcgcagc cgccagacat
     1981 gactgatgcc agctcgccag cgcccaccat cggtcggacg cgttgggcga ttgccgaggg
     2041 atatatcccg tcacagagca ccggcccgga gccggccatg accagccacg agacggcgtg
     2101 cctgctgaac gccggcggac aggacgcgca ggtgagcatc tggatctact tcgccgatcg
     2161 cgacccggcc ggcccgtaca ccgtccgcgt gccggcgcgg cgcaccctgc acctgcgctt
     2221 caacgagctg caggaccctg agccgctgcc gcgcgacacc gattacgcca gcgtcatcca
     2281 gtcggacgtg cccatcgtcg tccagcacac gcgcctggac tcccgccagg ccgagaacgc
     2341 catcctctcg acgatcgcct acgcgggcga atgaccgata cgcgtccctg ccgcgtcgtg
     2401 ctggtgggga tgatgggcag cggcaagacg accatcggaa gggcgttggc cgagcacacc
     2461 ggctggggct accacgacaa cgacgagctg ctggccgcag ccaccggccg gagtgcggcc
     2521 gagatcgcgc tgtccgggga ggccgccctg cgcagcgccg aggcggcggc cctggcagag
     2581 gcgctcagca tgccgccgcc cacgatcgtc gcggtggccg ccggcagcgt cctggaccgt
     2641 gaccaccgcg agcggctccg ccgcgacgcc ctggtggtct ggctccacgc cgcacctgag
     2701 cgcctggcga gtcgcgcgac cggggcggcg caccggccct ggctggacga cgatgcggca
     2761 cagtggttcg cccgcgccgc cgcggagcgc gagccgctct accgggcggt ggcggacctg
     2821 gtggtcgaaa ccggcgcgtc gggcccggcc gctgccgcgg agcggatcct cgactggctg
     2881 cgcggaacgg cgtgcgctcg gtgggtgccg cccgcccgag aggcccagcc gtagcccggc
     2941 acgctgcttg ccggatcagc cggcatggac gacaggaacc cacgccgcga ccagccactc
     3001 ccctccgagg acaccggcga cctggcggaa acgcctccca gcggcgagac cgatgacttc
     3061 ctggtggccc gcgaggaggg cgttccgtac gtgccgccga cagatcgggt gctcagccag
     3121 tcgcgccagg gcgagtccgg cccggaccag gcggccgccg cgccggacga cgcgtccgag
     3181 ctctcgcgcc aggcaccgat caccgatgac cagcaggacc tgaccgcccg cgcggccgag
     3241 gcgctgcgca tgtcggacgt gaccgccgga gaccggctgc gggtggggag cgtcggctcg
     3301 acggtctacc tgcgcggtca ggtggagtcg gtcgacatcg ccgacgagct gacctcgatt
     3361 ctcggcgacg tggccggcgt cgacgaggtc gtggacgaga cgcaggtgcg cgggctgtga
     3421 gccgaccgat ctggcacgcc cggtgctatc caatctcccc ggggaccggt gaaccgctcg
     3481 ccggacggga actcccgggg ccccgccgtc ggcgcccgcc ggcctccacc cggccggttg
     3541 ggcgccggcc acgtttcgcg aacgggagcc gtccatgagc accggcgcgg agcgcgagag
     3601 cggcgtgcag cgaccgaacg caccggatgc cggctggccg acagggatgc cgtacacggt
     3661 ccacttccca tacgagggcg attacctggg tgccgatctc gagcaccccg ccccgctgcc
     3721 acgggtcggc gacgtggtcg agtacttcga cgagcgcggc gcctgccacc ggttcagagt
     3781 cagggaggtg atccacacgc tgcagacctc tgccgcctac cgtcccgcgg tggcagacgg
     3841 gacagcatcc ccccagtcgg tggcgcggct ggacgaggac gtggaagccg agccgccggg
     3901 cggatccggc gtggtgcgcg caggcctgcc caaggtcatc ctggagacgg ttcgggagga
     3961 gtaggccgcc gggctgtgga tccatggcct gtgacgacgg ccggcttgcc gcgacgccca
     4021 cccggcgctg ggggtggaac acggcgcgcc gaccgttggg ggcgccgatt ggagcatgcc
     4081 gcgtgccagc caggaggcct ttgattgggc acggcaggtg caccacgccg gccccatgac
     4141 aaccatcggc tacgcgctca gcagcgaaga gcatccgccg cgcaacctgg tcgggcacgc
     4201 ggccgcggcc gaacgggccg ggttccgctt cgcgctcatc tcggaccact tccatccctg
     4261 gaccagccgg cagggccaca gcccattcgt gtggtccgtc ttgggcggca tcgcccataa
     4321 cacctcgagc ctggaggtgg gcacaggcgt gacctgcccg ctgatccgca cccacccggc
     4381 catcatcgcg caggcggccg ccaccagcgc gtccatgttc gagggccgct tcttcctggg
     4441 cgtggggacc ggagagaacc tgaacgagca catcttcggc gatgcctggc cgcgttacgc
     4501 gacgcggaac gagatgctgc gcgaggcggt cgagatcatg cgcgagctgt ggaccgggga
     4561 gctgaccagc tacgagggcg cctactatcg cgtcgagaac gcgcagctct acaccctgcc
     4621 ggagcgcccg ccagcggtga tggtggcggc cagcggcccc gaatccgcgc agctggccgc
     4681 ggagatcggg gacgggatga tcaccaccac gccggacaag gagctggttg atgcctttcg
     4741 ccaaagtggc ggagaaggga agccgcttta cggccagctg accgtgtgct gggcgccgac
     4801 gcgcgaccag gcgctggacg tggccatgga atggtggccg accgccggcg tccccggcga
     4861 cctgtcacag gagctgccgc tgcccatgca cttcgagcag gtcagcaagc tggtcacccg
     4921 ggatcagctg gcgcagagcg tcctgtgcgg tccggacgcg gacgagcacc tgcagcgcat
     4981 cggtcagttc acagaggcag gcttcgacca cgtctacgtc caccaggttg gcccggacca
     5041 ggaaggcttt ttccgcttct acgccgacca ggtcctcccg cggctgacct gagcgacgga
     5101 cccgcccgct ctggcacacg ccctgctcaa tgccctgcca tggcccatcc tccgctcgtc
     5161 cacgtcaaga cctgcaaccg ctgcgggcgg acgggctcgc acgccttcag gggcacgccg
     5221 gacgggctct acgaatgcac cacggtgacg gcctgccgcg cccgtacgcg gcgcaacgcc
     5281 ggcctgcggg gggaggggcg gggccggctg ccgaagcagg cgggcctgaa cggcgcacct
     5341 cccggcgtcg cctacgtcat cggacaggcg gacgcaccgc gggcgttggt ggaggagacg
     5401 ctgcgggacg cgacgccgct gtcagtggcc gtcggcgagc cgaaccgggc cacgttgacc
     5461 gcgcttggct cgcgcaacgt caagctcatc gccatcgatg cccgctgcct ggcgcgcatc
     5521 ggttttcgca acgagttcag cctgcggcga cgccagccca ggctgagctc agtaccggta
     5581 ttcctgtacg gcgacgacgc gacgctggcc cccatggccc cgcagttccc agacgcccgg
     5641 ctgatcggct tcgcgaacgg accgcgagaa gcgatccggc ggctgcgccg cgaggtgcgg
     5701 cttcccgaag ccgccgtgcg ggcgagggcc tgagctaggg catcagctcg tcgtccgggt
     5761 tgacgtccgt ctcgcgatgg ccctgcggcc actcgtcatc ccggccctgg acgcggtgct
     5821 ccttggcgcc ggtgccgctc gactctcgat cgtggccggc aacccgctcg tcgtcctcct
     5881 ggacgccgcc cgggacggtc tcggcggccg cctcgccgcg gttgttgata aaggcgcccg
     5941 catcgggctg gttggtgcgc tcacgttcgc tcatgatggc ctgcggactg cagcatcggt
     6001 gccgggggcc tgcagcgggt ggcgcgcccc ttgcatgctc gccgagcaat ggtcgaggca
     6061 cactcccgtc cggcctggta cagccaggcc gtcatctatg ccatcgacgt cggcagctat
     6121 cgcgactccg acggggacgg cgtcggcgac ctggtcggcg tgagccaggc gctcccctac
     6181 ctgcaacgcc tcggcatcga ctgcatctgg cttctgccct tcttcgccac cgagaaccgg
     6241 gacaacggct acgacgtcac cgactactac gccgtcgacc cgcagttcgg gaccttgggt
     6301 gacttcggtg acctggtgga cgaggccgag cagcgcggca tccggatcgt gatcgacctg
     6361 gtggtgaatc acacctcgga ccgccacccg tggttccagg ccgcccgcag ccgcgacccg
     6421 tccccgtacc gcgactacta catctggaac gaccaaccgc cgccgctgcg ggagcagccg
     6481 ccgtccatct ttcccgaccg cgtgccgtcg gtgtggacgt gggacgagca gcgcgggagc
     6541 ttctactacc accagtttta cccgtttcag ccggcgctca accacctcaa tccggacgtg
     6601 cgcgacgagg tgcgacgcat catcgccttc tggctccgcc ttggagtcgc cggcttccgg
     6661 ctggacgcgg cctcgcacct catcgatccg ccgctggacg gcgagccggc ggaccgcgtc
     6721 gagatcctgg gggagtaccg ccggcacgtg gtcaagggca ggggcgacgc gatcctcatc
     6781 ggcgaggcgg acctgccgga gaaggacctg gagccgctga tgggtgagca cgctttgcag
     6841 ctgctgttca acttcgtcct gaatggacac gcctttctgg cgatggcacg cggcagcgcg
     6901 gaaccgatcg tggaggcgat tgggcgcctg ccgcacccag cggatgccgg gtgggcgaac
     6961 ttcctgcgca acctcgacga ggtggacctg gaacggttga ctgatgcgga gcgcgaggag
     7021 gtgtttgccg ccttcgcccc cgacgagggg atgcgcatct tcaatcgtgg gatccggcgt
     7081 aggctggcgt cgatgctcga cggcgaccag cgacggctcg agctggcgta cgccatcctg
     7141 ctggcgctcc ccggggcgcc ggtgatcgtg tacggcgacg agatcgggat gggcgacgac
     7201 caatcgctgc ccgagcgtga ggcggtgcgg accgccatgc agtggacgtc cggcacgcga
     7261 gccggattca ccccggccga caccaaccgc cgcagcgtga aggtcatcag cggcgggcgc
     7321 ttcggcgccg agcgcgtcaa cgtcgctgac caggagaacg atcccggttc attgcttcgc
     7381 tggctggagg aggcgatccg ggtgcgacga gcgacgcctg agctggtggc cggcggagct
     7441 gaggtcacgg cactggcgga tggtgccgtg ctggcgcgtc gctgcgagct ggacggcgtc
     7501 cgcgtggtgg cgctccacaa cctggccggg caccaggtca gcctcagccg cgacgagctc
     7561 ggcctggagg ccggtcccgt acagcggctg ctggccgacg gacggtcgcc agatccgacg
     7621 gcgggcaaac gggcggtaag actggcgccg tacggctacc tctggctgcg catggaatgg
     7681 agtgacgaag aggccggatg accgattcgc acctgtggtg gcaaaccggc gtgatctacg
     7741 agatctatcc gcgcagtttc gcggacagcg atggcgacgg ggccggcgac ctgccgggcg
     7801 tgcgcgggcg gctggattac ctgcgctggc tgggcgtgga tgccatctgg ctcacgccgt
     7861 tctacccctc ccccatggtg gacggcggct acgacgtggc cgattacacc gatgtccatc
     7921 ccatgttcgg cgatctccag cagttcgatc tgctggtggc cgaggcccat cgccggtcgc
     7981 tgcgcgtcat cgtggacctc gtgccaaacc acacctcgga ccagcacccg tggttcatcg
     8041 agtcgcgaag ctcacgggac aacccgcgtc gcgactggta catctggcgc gaccctgccc
     8101 cggatggagg cccaccgaat aactggatca gcgagttcgg cggttcgggt tggacactcg
     8161 acgagagcac cggccagtac tactaccacg ccttcgcgga ggcccagccg gacctcaact
     8221 ggcgtaaccc cgatgtgcgc cgggcaatgt acgacgtgat gcggttctgg ctggatcggg
     8281 gtgtcgacgg ctttcgcgtc gacgtcatgt ggcacgtcat caaggacgag ctgctgcgcg
     8341 acaacccgcc caaccccgat taccgcgagg gcgtcgactc gccatcgcgc cgcctcatcc
     8401 aggcctactc gaccgatcag cccgaggtgc acgatgtcgt cgccgagatg cgcggagtgc
     8461 tcgacgagta cgacgagcgg gtgatgatcg ccgagatcta cctgcccgtc gaccgcctcg
     8521 tcgactacta cgggcgcggg gagcgggccg gggcgcacct tccgttcaac ttccacctca
     8581 tcctcacgcc ctgggacgcg cgggagatcg agctggtggt ggaccgatac gagggcgccc
     8641 ttcctgccaa cgcctggccg aactgggtgc tgggcaacca cgaccttccc cgggtggcca
     8701 gccgcatcgg tcccaaacag gcgcgcatcg ccgccatgct gctgctctcc ctgcgcggca
     8761 caccgactct ctattacggg gaagagctgg gcacggagga gacgcccgtg ccgccggagg
     8821 acgtccagga cgtacgtgag atcaaccagc ccggccgagg cctggggcgt gacccgtgcc
     8881 gcacgccaat gccctgggat ggttctcccg gtgccgggtt cacgaccggg cgaccctggc
     8941 tgccgatcgg cgaggacaac cgccagcgga acgtggaggc cttgcgatcc gacccggact
     9001 cgatgctctc cctctaccga cgcctcctcg agctccgtcg ccgggagccg gccctgacca
     9061 tcggcagcta ccgcccggtc ccggccagcg ggtcggtcct ggcgttcgag cgcgagcagg
     9121 acggccggcg cctcctggtg gccctcaacc tctccgacca gcccgcaggg ctggagctgg
     9181 agccggatgg tcgccgctac cgtcctctgc tctccaccct ccccgacgcg gcaggtgggg
     9241 aggtcgggcc ggcgatcgag ctgcgcgcgg acgaggggct catcctggaa ccgctcgaat
     9301 agggcaggcc ggcagcgagc ggccacgtgg caagatcggg cgcatggacg atccgcccgc
     9361 cgcagcgccg ggcagctggg gttcggtgct cggcgtcgcg ctgcagctgg gcctgacctc
     9421 cttcggcggg cccatcgcgc atctcggcta cttccgccgg gagtacgtcg agcgccggcg
     9481 gtggctggac gaacgtgact tcgccgacct ggtggcgttg tgccagacgt tgcccggtcc
     9541 ggccagcagc cagctcggaa tcgccatcgg caggctgcgc gccggggcgg cgggcgcact
     9601 ggccgcctgg gtcggcttca cgctgccatc ggcggcgatc atggtcggct tcgccctgct
     9661 ggccggcagc accgacctgg ccgatgccgg cggggtcgcc gggctgaagc tggccgcggt
     9721 ggcggtggtc ggccatgcgg tgctgcggat gtcggcgacc ctcgccccga acctggcgca
     9781 gcggctcctg gcactggtgt cggcgggggt cctgctggtc ctcgaaggcc cggtcatgca
     9841 ggtcggcgtg ctggccgccg cggcactcct ggggtatgcc tggacgcacg gccagcgccg
     9901 gagtcccccg acggctcgcg tcggagggcg caggtggggc atggtggcgc tggtcgtgtt
     9961 cggcgtgctg ctgatcgcgt tgccggtgct ccgcgcggtc gacggccagg tcgtggcgct
    10021 ggtcgacact ttctaccgcg ccgggtcgct ggtcttcggc ggcggccacg tggtgctgcc
    10081 cctgctcgac gccggggtcg tcgggccggg ctgggtcagc gaggaccagt tcctggcagg
    10141 gtacgggctg gcacaggcga tccccggacc gctcttcacc ttctcggcct acctgggagc
    10201 ggtggccgcg ccggaaccga acgaggtcct gggagccgtg ctggcactgg ttgccatctt
    10261 cctgccctcg ttcctgctgg tcttcggcgt cctgcccttc tgggagcgtc tgcgtggctc
    10321 gccgcgggtc cggggtgccc tggatgcggt gaacgtggcc gtcatcggac tgctgctggc
    10381 ggcgctgtac cacccggtgg cgaccgcagc cattggctcg ccgctcgacg cggccgtggc
    10441 gctcggcggc ctggccctgc tcagcgcgcg ggtgcctccc ctggccgtcg cggcgggggc
    10501 tgccgtcgcc ggccagctca tcggtccttg agctcgtgcc accctcgatg cagtccgggt
    10561 gcgctggagg ctaccgtgtc ccgtcggcgc ttgagccaga gtcgcgacga ctgacgcatt
    10621 gaccggctgc tcatactagg ggggagtatc caccaagcca gggagcggga gcccagcgtg
    10681 tcagagcatc accaggacca tcaggcgcac gacgagcacc acgcggcgtt gccttccgat
    10741 ccgcatcggg cgccgctcga tgccggtgcc tccacgagcg acgcccatgc gggccacggc
    10801 ggagccgatg gcggcggtca tggcggtggt gcccatggcg gcggagccca cggtggacac
    10861 gacaagcacg cgggccacga cccggaggcc tttcgccggc agttctggct cgtcctgctg
    10921 ctgaccatcc cggtcgtggt ctggagcgag gaggtccaga tgtggctggg ctaccgcgcc
    10981 cccgccttcc ccggctcgga gtgggtggcg cccgtgctgg gtaccctcgt cttcctgtac
    11041 ggaggtcgcg tcttcctgtc gggagcgcgg acggagctgg gcgaccggca gccgggcatg
    11101 atgaccctga tcagcctggc gatcgtggtg gccttcgtca ccagctgggc ggcgacgctc
    11161 gggctgttcg aggtcgaggt gtggtgggag ctggccaccc tgatcaccat catgagcctc
    11221 ggccactggc tcgagatgcg atccatcatg caggcccgcg gcgcgctcag cgcgctcgcc
    11281 gagctgctgc ccgacaccgc ggagcgagtc acccccggcg ggctggagac cgtcccgctg
    11341 gatgcactgc agacgggcga cgtcgtgctc gtgcgtccgg gggggcgagt gccggccgat
    11401 ggcaaggtgg cggagggaag cgccgacgtc gacgagtcga tgatcaccgg cgagtcacgg
    11461 ccggtcaccc gcggaccggg ggacgcggtc accgctgcca cggtcgcggc gggcggcagc
    11521 ctgcgggtgc gcgtcaccgc cgtgggggac gagaccgcgc tgtccgggat catgcggctg
    11581 gtggccgcag cgcaggcctc gagctcgcga gcgcaggcgc tcgccgaccg tgccgctgcc
    11641 atcctcttct acgtggcact ggtcgcgggc gggctgaccc tggccgcctg gtcgctgctc
    11701 ggcgacccgg aggagggtct catccggacc gcaaccgtgc tggtcatcgc ctgcccgcat
    11761 gcgctgggtt tggccatccc gctggtgatc gccatcggga ccacgctcgg tgcgcgcaat
    11821 gggctgctga tcaaggaccg gttggccctg gaacgagccc gcgagctgga ggtggtcgtc
    11881 ttcgacaaga ccggcaccct cacccgggga cgaccggtgc tggcagccgc ggccccggca
    11941 gccggcgtgg acgaaggtga gctgctcgag atcgcggcgg cggtggaggc ggacagcgag
    12001 catccgctgg cccgggcggt ggtggctgcc gcccaggaac gcgggctgcg accacccgcg
    12061 gtggagagct tcgaggcgct tcccggacgg ggtgcgcgtg ccaggctccg cggcgtggag
    12121 ctgcgcgtgg gtggaccggc cctgctggct gatgccgggg ccgagctccc ttccgagatc
    12181 gcctcagaga cggaggagtg ggcagcagcc ggcaggacgg tgctctacgt gctgcgcgac
    12241 ggggtggtgg tgggagcgat ggccatggcc gacgagatcc gccccgaatc ggtcgaggcg
    12301 gtcaggcgcc tgcacgagat gggtctgcgg gtggccatga tcaccggcga cgcgcagtcc
    12361 gtggcggacg acgtggcgcg ccgcatcggc atcgacgagc tggcggccca ggtgctgccc
    12421 gccgacaagg cggccgcggt ccagcgcttc caggccggtg gccagcgcgt ggcgatggtc
    12481 ggcgacggcg tgaacgatgc gcccgcgctg gcccaggccg atgtcgggat cgccatcggt
    12541 gccgggacgg atgtcgccgt cgagtcggcc ggcatcgtgc tcgtccgtga cgaccctcgg
    12601 gacgtgcccg gcgccatcga gctcagccgg gccagctacc gcaagatgat ccagaacctg
    12661 gcgtgggcca cggcctacaa cgcgatcgcc atcccggtcg cggccgggtt attcgcgccg
    12721 tgggggatcg tgctggccat gagcgtcgga gcgctggtca tgagcctgtc gaccatcatc
    12781 gtggcggcga acgcgcagct gctgcgcggg ctgaggctgc gtccggagcc gtcggccctg
    12841 gcggccacgc cgggctgacc tacggttcgc ggacctcgag cacctccccg gcagccaggt
    12901 ccacgaccac gtccatcgac ggcgagccct cctccccgcc ggagcggtag gcgaccacca
    12961 tgaggtgcgc gttgggcggc ggctgccaag tggcaccctc ggagctgtcc ggcctcgcct
    13021 atgccggtgc cactt
//
LOCUS       DATACA010000158         6427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1708560, whole genome shotgun sequence.
ACCESSION   DATACA010000158 DATACA010000000
VERSION     DATACA010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708560"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VHK28_03235"
     CDS             complement(<1..377)
                     /locus_tag="VHK28_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2141280.1"
                     /translation="MTDTRPARPRFQPAADVLSGDTADVYFDRAQRILAAEQLDPVVT
                     MEIFPREDAILCGAEEALAYLREIFAEANGDTEPVVESLHDGDRFSPKEVVMRIQARY
                     SAFGLYETAILGILSQSTGWATAA"
     gene            complement(374..2239)
                     /locus_tag="VHK28_03240"
     CDS             complement(374..2239)
                     /locus_tag="VHK28_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141281.1"
                     /translation="MVTQATYSVLPDAREIAVSIQVEFTNTTPDPAGQFSVFEVIDLA
                     IHSGATEVAARDSRGSLNVAAAEDDGVNVASVQPRTPVRFEQEMSFTLSYVIPDGAAP
                     DVRVRPSIVIFPAWSFGTQGTVSVDVPVAYEVLVDGDELTAARDGASWRLQSGEVADP
                     TRWLALVTVSHPDAYATLSRVVPLGSGTVDLQVRHWSDDEAWGEATADVLTRGLPALE
                     EALGLSYQRVGPLTVVESLPTSDGGLGETEAGDSVVLAVSFDADPFTILHQAAHVFVT
                     DALAEDRWIREGFASWAASRAGAEQEAEAPWDPEARLEETGEAAFPLVSWGAGQATAE
                     QDAYAYAASWALTERLTETIGEEDVRLAWNRIAAGIGPYEPPSDEPVEPDGRVPVAID
                     SRQLLDHLETVSGQDLAEAFVELVFDQATAEQMTARGAARKAYAALLAAADEWGGPEP
                     VQAALAGWRFDEAQQRMTEAAEWLVARDRLLADAERVGLAVPARLRDQWQTAGGGPGA
                     QTELDAERSVVDAYATAHERNQEERSIFDGIGLLGGPQPGDELTRARALFAEGELRGA
                     LKAAQSASLRLERATTDGVLRLASVLVVLGLIVAAAVYLARRRRTRPASVYTAAP"
     gene            complement(2353..2940)
                     /locus_tag="VHK28_03245"
     CDS             complement(2353..2940)
                     /locus_tag="VHK28_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141282.1"
                     /translation="MAGADERWSPPIDAWLSELGVEVVSGAGGPQDAAFSRDVVLDGA
                     RRFDLRVTVAWVEGIGASLWAYYGPEAMEIPKRVYHRMLRAAFDYPFVKFAMTDDDRP
                     MLMTELPPSAVTRDELGRGLVRLAVVGDRLLEETAAAVADRGQLPDWSGRQARNAALL
                     DAYRDEVEAAMPTWDPPPQRSRRPGLLARLLGGGR"
     gene            complement(2945..4204)
                     /locus_tag="VHK28_03250"
     CDS             complement(2945..4204)
                     /locus_tag="VHK28_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX2141283.1"
                     /translation="MSTSVLTLILAGGEGERLSILSQVRAKPGVPFGGKYRIIDFSLS
                     NAVNSALTDVGILTQYAPRSLMDHIGLGRPWDLDRTHGGVTLLQPFIGRGRARDWYRG
                     TADAVLQNLEFIADRDPELVLVLAGDHVYKMDYRPFIATHRASQADVTCAVRTVPLEE
                     AHRFGILETDEQGRVVAFTEKPPNPTSNLVSMGVYVFSWSFLRDALSPERVDFGRDML
                     PWMVAGGQRVQAYEFRGYWEDVGTVESYWRANLDLLSDAPGIELNDLGWLVYTKSEER
                     PPARIGPEAHVERSMISHGCVIAGSVIHSVLSPGVRVEQGAVVRDSIVMFDSVVEEGA
                     VLDRVIVDKECRIGRGAVVGRGDELRPNRDEPERLYAGITLVGKRASVPPRLAIGRNC
                     RVDPGVGPADFGRRRVVRSGETVARPG"
     gene            complement(4186..>6427)
                     /locus_tag="VHK28_03255"
     CDS             complement(4186..>6427)
                     /locus_tag="VHK28_03255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2141284.1"
                     /translation="SGRGYDDKVREFILARQMTDAYPGRQGKEQILETYLNLIFYGNG
                     SYGIQAATANYFGISDLTKLSLAQSAFLAGLPQLPSAYDPYFNDQGPARAIARRNVVL
                     DAMLRDGYITPAERDQASATTWEEMQPKRISAILREPHFSLRVSREAERIFEALGKAN
                     PAQEVRTGGYRITTTLDYGLQDVAKDQVTAWVNHPELKNKNVNNGALVAIDSRTGEIV
                     AYVGSVDYYLRDDPRVQGQFDVAGQGVRQPGSAFKPITYSSAFVAREATPATFFVDAT
                     TQFGPERATSYLPTNADIQEHGPLLAVDALRYSLNVPSVKMQHLVGAQATAEFAERMG
                     IASAEYIMDQDPGLTLALGSVPVSLTNMTGAYSVFAAEGARHPPTTILEIRDRNNRIV
                     YTREDNGPQTGQPMTAAEAYLTHWILDGNTDPRRNVIWGSRAQLTDAAGNRREAGFKT
                     GTTNDFRDVSGFGYVPGSLVTGVWMGNNNQEPMSNVLGRGLFSADGPLYLWHDFMQIA
                     LNQPWDWNGQQPVPQTTFERPSGITMANICQFSGMRATNGCGPTREMPFVEGTVPPPD
                     DLHSKGCLDIVQAVARDDRRPDEWVEAAQTWADRLVNGQTGAVGDPTKLKENPNYRLR
                     ISPVPGNNGYGGSICGRLRATPRPAPTAPPSGGGPSKPPDGPSPCPPPRNECDDDDRP
                     TLPVEPAGEAGAQPAATDVALAPSLGLVALLWATPAVGSVASSRRRRRRRGTGVSESG
                     ACPPRS"
BASE COUNT          909 a         2321 c         2233 g          964 t
ORIGIN      
        1 gcggcggtgg cccaccccgt ggactggctc aggatcccca ggatcgcggt ctcgtacagg
       61 ccgaaggccg agtagcgcgc ctggatgcgc atcaccacct ccttcggcga gaaccgatcg
      121 ccgtcgtgca gcgactcgac gaccggctcc gtgtcgccgt tcgcctcggc gaagatctcg
      181 cgcaggtagg cgagagcctc ctccgccccg cacaggatgg cgtcctcacg cggaaagatc
      241 tccatggtca ccaccgggtc cagctgctcg gcggccagga tccgctgcgc ccggtcgaag
      301 tagacgtccg ccgtgtcgcc ggacaggacg tcggccgccg gctggaaacg tggacgggcc
      361 gggcgcgtgt cggtcatggc gcagcagtgt agacgctggc cgggcgcgtt cggcgccggc
      421 gggccaggta caccgccgcg gccacgatca gcccgagcac caccaggacc gatgccaggc
      481 gcaggacccc gtcggtggtg gcgcgctcca ggcgcaggga ggcggactgg gcggccttca
      541 gggccccgcg cagctccccc tcggcgaaca gcgcccgggc gcgggttagc tcgtcaccgg
      601 gctgcggccc gccgagcagg ccgatgccgt cgaaaatcga acgctcctcc tggttgcgct
      661 cgtgggcggt cgcgtaggcg tccaccaccg agcgctccgc gtcgagctcg gtctgggcgc
      721 ccggtccccc gcccgcggtc tgccactggt cgcgcagccg ggcggggacg gccaggccaa
      781 cgcgttcggc gtcggccagc agccggtcgc gggccaccag ccactcggcc gcctccgtca
      841 ttcgttgctg cgcctcgtca aatcgccagc cggccagggc ggcctggacc ggctccgggc
      901 cgccccactc gtcggcggct gccagcaggg ccgcgtacgc cttgcgcgcc gcgccgcgcg
      961 ccgtcatctg ctcagcggtg gcctggtcga acaccagctc cacgaacgct tcggccagat
     1021 cctgcccgga caccgtctcg aggtggtcga ggagctgccg ggagtcgatg gccaccggca
     1081 cgcggccgtc gggttccacc ggctcatcgg agggcggctc gtacgggccg atgcctgcgg
     1141 cgatgcggtt ccaggccagc cgcacgtcct cctcgccgat cgtctcggtg agccgctccg
     1201 tgagtgccca ggaggcggcg taggcgtagg catcctgctc ggctgtggcc tggccggcac
     1261 cccagctcac cagagggaag gcggcctcac ccgtctcctc caggcgggcc tcgggatccc
     1321 acggcgcctc ggcctcctgt tcggcgcccg cgcgcgacgc tgcccacgac gcaaagccct
     1381 cccggatcca tcggtcctct gccagcgcat cggtcacgaa gacgtgcgcc gcctggtgga
     1441 ggatggtgaa cggatccgcg tcgaaactca ccgccagcac cacgctgtcg cccgcctccg
     1501 tctcgccgag ccccccatcg ctggtcggca gcgactccac cacggtgagc ggccccaccc
     1561 gctggtagga gaggcccaac gcctcctcca gggccggcag gccgcgcgtc agcacgtcgg
     1621 cagtcgcctc gccccacgcc tcgtcatccg accagtggcg gacctgaagg tccaccgtcc
     1681 ccgaaccgag cggcaccacg cgtgacagcg tggcgtaagc gtcggggtgg ctgaccgtga
     1741 ccagtgccag ccagcgggtc ggatccgcca cctccccgct ctgcaggcgc cagctggcac
     1801 catcgcgggc cgctgtcagc tcatcaccgt ccaccagcac ctcgtaggcg accggaacgt
     1861 ccacggacac ggtcccctgc gtcccgaagc tccacgccgg gaagatgaca atcgagggcc
     1921 gcaccctgac gtcgggtgct gcgccgtcgg ggatgacgta ggacagggtg aaggacatct
     1981 cctgctcgaa gcggacaggg gtgcgcggct gcacggaggc cacgttgacg ccatcgtcct
     2041 ccgcggcggc cacgttcagc gatccgcggc tgtcgcgggc ggccacctcg gtggcgccgg
     2101 agtgaatggc gaggtcgatc acctcgaaca cgctgaactg cccggccggg tcgggcgtgg
     2161 tgttggtgaa ctcgacttgg atgctgaccg cgatctcccg cgcgtcggga agaacgctgt
     2221 aggtggcctg ggtgaccatc gtgtactccg ggtcggcggc ccgcgccggc gcacgtcctg
     2281 gtacgacgcc agcaaccacg gcgagcagcg ctaacgccgc cagcagcctg cgcccgggag
     2341 cgcccgcggc tctcaccgac cgccgccgag caggcgcgcc agcaggccgg gccgcctcga
     2401 gcgctgtggc ggtgggtccc aggtcggcat ggccgcctcg acctcgtcgc gataggcgtc
     2461 gagcagggcg gcgttccgcg cctggcgccc gctccagtcc ggcagctgtc cgcgatccgc
     2521 caccgccgcc gccgtctcct ccagcagccg atcgccaacc accgccaggc gcaccaggcc
     2581 tcgccccagc tcgtcgcggg tgacggcgct ggggggcagc tcggtcatca gcatcgggcg
     2641 gtcgtcatcg gtcatggcga acttcacgaa ggggtagtcg aaggcggcgc gcagcatccg
     2701 gtggtagacg cgcttcggga tctccatggc ctccggccca tagtatgccc acagcgaggc
     2761 accgataccc tccacccagg cgaccgtcac ccgcaggtcg aagcgccgag caccgtcgag
     2821 caccacgtcg cggctgaacg ccgcgtcctg ggggccgccc gcgccggaca cgacctccac
     2881 cccaagctcc gagagccagg catcgatggg tggcgaccag cgctcgtccg cgcccgccac
     2941 cgagtcagcc agggcgagcc accgtctcgc cggagcgcac cacccggcga cggccgaagt
     3001 cggcgggccc cacgccggga tccacccggc agtttcgccc gatagccaga cgcggcggca
     3061 cggacgcccg cttccccacc agggtgatgc cggcatacag gcgctcgggc tcgtcccggt
     3121 tgggccgcag ctcgtccccg cggccgacca ccgccccgcg cccgatccgg cactccttgt
     3181 cgacgatgac gcggtccagc accgctccct cctccaccac gctgtcgaac atcacgatcg
     3241 agtcgcgcac gacggcaccc tgctccaccc gtacgccggg cgagaggacg ctgtggatca
     3301 cgctgccggc gatgacgcag ccatggctga tcatgctgcg ctcgacgtgg gcttcgggac
     3361 cgatgcgcgc cggcggccgc tcctcgctct tggtgtacac cagccagccc aggtcgttca
     3421 gctcgatgcc cggcgcgtcc gacagcaggt ccaggttggc ccgccagtag ctctccaccg
     3481 tgccgacgtc ctcccagtaa ccccggaact cgtaggcctg cacccgctgg ccgccggcca
     3541 ccatccaggg cagcatgtcg cggccgaaat cgacccgttc gggcgacagg gcatcgcgca
     3601 ggaaggacca ggagaagacg tagacaccca tgctcaccag gttgctggtc gggttgggag
     3661 gcttctcggt gaacgccacc acgcggccct gctcatcggt ctccaggatg ccgaaccgat
     3721 gcgcttcctc cagcgggacg gtgcgcacgg cgcaggtgac gtcggcctgg ctggcccgat
     3781 gggtggcgat gaacggccgg tagtccatct tgtagacgtg gtcgccggcc agcaccagca
     3841 ccagctccgg gtcgcggtcg gcgatgaact ccaggttctg gagcacggcg tccgccgttc
     3901 cccggtacca gtcgcgcgcc cgtccacgac cgatgaacgg ctgcagcagg gtcaccccgc
     3961 cgtgggttcg gtccaggtcc catggccttc caaggccgat gtgatccatc agtgagcgcg
     4021 gcgcgtactg ggtcaggatc ccgacgtcgg tgagggcgga gttgaccgcg ttcgacagcg
     4081 agaagtcgat gatccggtac ttgccaccga aggggacgcc cggcttggca cggacctggg
     4141 acaggatgga caggcgctca ccttcgccac ccgccaggat cagggtcagg accgaggtgg
     4201 acacgctccc gattctgaca cgcccgtgcc acgccgccgt ctgcggcggc gtgagctggc
     4261 cacgctgccc accgcgggcg tggcccacag cagcgccacc agcccgagcg atggggcgag
     4321 cgccacgtcg gtcgccgccg gctgcgcacc cgcctcgccg gccggctcga ccggcagcgt
     4381 cggtcggtcg tcgtcgtcgc actcgttgcg cggtggcgga cagggcgatg gtccgtcggg
     4441 cggcttgctg ggtccgccgc ctgacggagg ggccgtcggc gccggcctgg gcgtcgcgcg
     4501 caagcggccg cagatggacc cgccgtaccc gttgttgcct ggcaccgggc tgatacgcag
     4561 gcggtagttc gggttctcct tgagcttggt cggatcgccg accgccccgg tctgcccgtt
     4621 caccagtcga tcggcccagg tctgcgcggc ctccacccac tcgtccggcc gtcgatcgtc
     4681 gcgggccacg gcctgcacga tgtccaggca gcccttgcta tgcaggtcgt ccggcggcgg
     4741 cactgtgccc tccacgaacg gcatctcgcg cgtgggaccg cacccgttgg tggcccgcat
     4801 gccgctgaac tggcagatgt tggccatggt gatgccggat gggcgctcga aggtcgtctg
     4861 cggcaccggt tgctggccgt tccagtccca cggctggttc agcgcgatct gcatgaagtc
     4921 gtgccacagg tagagcggcc cgtccgcgct gaagagtccg cgacccagga cgttggacat
     4981 cggttcctgg ttgttgttcc ccatccagac gccggtcacc aggctgccgg gaacgtagcc
     5041 gaagccggag acgtcacgga agtcgttggt cgtcccggtc ttgaatccgg cctcgcgtcg
     5101 gttgccggca gcatcggtca gctgcgcgcg ggaaccccag atcacattgc ggcgcgggtc
     5161 ggtgttgccg tccaggatcc agtgggtgag gtaggcctcg gccgccgtca tgggctgccc
     5221 ggtctggggg ccattgtcct cccgggtgta gacgattcgg ttgttgcggt cacgaatctc
     5281 caggatggtg gtgggcgggt gccgcgcgcc ctcggctgcg aacaccgagt aggcgccggt
     5341 catgttggtc aggctgaccg gcaccgagcc cagcgccagc gtcaggcccg gatcctggtc
     5401 catgatgtac tcggcgctgg cgatgcccat gcgctccgcg aactcggctg tcgcctgcgc
     5461 cccgaccagg tgctgcatct tgaccgacgg cacgttcagc gagtagcgca gcgcgtccac
     5521 ggccagcagc gggccgtgct cctggatgtc ggcgttggtg ggcaggtagc tggtggcccg
     5581 ctccgggccg aactgggtcg tggcgtccac gaagaaggtg gccggcgtcg cctcgcgcgc
     5641 cacgaatgcc gacgagtagg tgatgggctt gaaggccgat cccggctgcc ggactccctg
     5701 gccggccacg tcgaactggc cctggacccg tgggtcgtcg cgcaggtagt agtccacgct
     5761 gccgacgtag gccacgatct caccggtccg cgagtcgatg gccaccaggg ccccgttgtt
     5821 gacgttcttg ttcttcagct ccggatggtt cacccaggcg gtcacctggt ccttggccac
     5881 atcctgcagc ccatagtcca gggtggtcgt gatccggtag ccgccggtgc ggacctcctg
     5941 cgccgggttg gccttgccca aggcctcgaa gatgcgctca gcctcgcggc tcacgcgaag
     6001 gctgaagtgc ggctcccgca ggatggcgct gatgcgcttg ggctgcatct cctcccaggt
     6061 ggtggcggac gcctggtcgc gctcggcggg ggtgatgtag ccatcgcgca gcatggcgtc
     6121 cagtaccacg ttgcggcggg cgatggcccg cgccggaccc tggtcgttga agtaggggtc
     6181 gtaggcggac ggcagctgcg gcaggccggc caggaacgcg gactgcgcca ggctcagctt
     6241 ggtcaggtcg gagatgccga agtagttggc ggtggccgcc tggatgccgt acgagccgtt
     6301 cccgtagaag atcaggttca ggtaggtctc aaggatctgt tccttgccct ggcgcccagg
     6361 gtaggcatcg gtcatctggc gggccaggat gaactcgcgg accttgtcgt cataacctcg
     6421 accggag
//
LOCUS       DATACA010000159         4758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_423569, whole genome shotgun sequence.
ACCESSION   DATACA010000159 DATACA010000000
VERSION     DATACA010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423569"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VHK28_03260"
     CDS             complement(<1..601)
                     /locus_tag="VHK28_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEX2141285.1"
                     /translation="MRRLRQDVDWLVSVGLLLSVVVTGATGVIADLWDLNDFWWHTVA
                     GYVMGGLAVAHVALNVGRLVSYGRFRVRREPARAAPTSVQRKERKAEAPMPWWSARGL
                     LSRRGFIGLAAGGALGWLTGRGLRQPPQIAAGSDVGVVYHEWSKPGIIDALGSVANWG
                     QFPELYKTYPDASKVVLPDPALDGDARTSRAIAGRRSIRH"
     gene            complement(652..1020)
                     /locus_tag="VHK28_03265"
     CDS             complement(652..1020)
                     /locus_tag="VHK28_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2141286.1"
                     /translation="MKTSRLSETAENAADPQHFTGPASRHDLIAVDDPPSSALLVRFG
                     PGGRTHWHRHPTGQYLYVVEGKGVAQSRGEPPVELAPGTCLYAAPGEEHWHGASPDEG
                     VAHLAFSFGVTEWVGPVEPE"
     gene            1170..1394
                     /locus_tag="VHK28_03270"
     CDS             1170..1394
                     /locus_tag="VHK28_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141287.1"
                     /translation="MPNDQAVEETVHYPNGKTKYTGFLLDGQMHGAWSWYRTDGSLMR
                     TGEFDRGQQVGSWRTFSRDGRVMKETSFHR"
     gene            1391..2590
                     /locus_tag="VHK28_03275"
     CDS             1391..2590
                     /locus_tag="VHK28_03275"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2141288.1"
                     /translation="MTERHSYLHSLECSSCGVVLDANQLHGVCPECGLVLLARYDLET
                     LRNDMPQPSFEGRRTDLWRYHELLPVSDPAHTDGLGEGGTPLLRLTGRAASQVGMDGG
                     ELFVKEEGLNPTGSFKARGMAVAVTRAAELGVTDVALPSAGNAAGAAAAYAAATGLGA
                     HVAMPSDAPPANLAEAATYGAEVMLVDGLIDAAGRLIRELAAEHGWFDLSTLREPYRV
                     EGKKTMGIELAEAGGWGDDWCPDAIIYPTGGGTGIVGMWKGFDELEQLGWIGPRRPKM
                     VVVQATGCAPMVRAFENGADRAEPWTDAHTIAAGMRVPSAIGDYLVLRAIRDSGGTAV
                     ALDDETIVDAQLRLARATGIYPAPEGAAAYAAVGRLRETGFLAGHERVIVFNTGTGLK
                     YPPPAMA"
     gene            2631..3542
                     /locus_tag="VHK28_03280"
     CDS             2631..3542
                     /locus_tag="VHK28_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2141289.1"
                     /translation="MPDRSRSEQLTLAAFLLLVVLAGGNAVGIDIASQELDAFWAAGT
                     RFAAGGLIFAILMGVFSVALPTGGALIGALLYGVLGFGAAFGLAFVAIPMTGAGTGQL
                     LLGLVPLLTVVLARLHGLEQIRLQSVIGSVVALGGVAILAADRIAADIPLLGIGLAFL
                     GALFLSEAGVIVKLTPRAHPIATNAVGMLTGAGLLLPVSLLLGETWTLPDQADTWAAM
                     TYLVVAGSVAVFALNVYVLGRWPASVVAVEFLLIPLATIPFSAVLTEEGITPIMLLGG
                     AVILSGVYFGALGPELGRGREARVADT"
     gene            complement(3547..3921)
                     /locus_tag="VHK28_03285"
     CDS             complement(3547..3921)
                     /locus_tag="VHK28_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX2141290.1"
                     /translation="MAENPWRKARPEDVERFEEAIGGIEGIEQRKMFGFPAAFVGGNM
                     VAGLHQDTVMVRLPEDERQERLDAGWSMFEPMPGRPMREYVALPAEVFADADATRGWI
                     ERAAEYARTLPSKEPKPRKRRA"
     gene            complement(3948..4613)
                     /locus_tag="VHK28_03290"
     CDS             complement(3948..4613)
                     /locus_tag="VHK28_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX2141291.1"
                     /translation="MSTKIGRPSRQRLPLSRERVLRAGIELADEEGIEQLSMRRLAQP
                     LGVEAMSLYHHVANKDDLLAGMLDIVFAEMELPSEEGDWKAALRRTAISAHHVLLRHE
                     WACGLLMQPTGPSAARLRWMNSVLRRLRHAGFSADMTHHSYHALDSHIVGFTLWVLPY
                     LKVAREQPGLVQDFISRVSVDDLPYLAEHIGVHMSDRPGETSEFDFGLDLILDGLERE
                     LRG"
BASE COUNT          716 a         1661 c         1593 g          788 t
ORIGIN      
        1 aatggcggat cgatcgacgg ccggcgatcg cccggctggt ccgcgcgtca ccgtccagcg
       61 ctgggtccgg gagcacgacc ttcgacgcat cggggtaggt cttgtacagc tccggaaact
      121 ggccccagtt ggcgaccgat cccagcgcgt cgatgattcc cggcttgctc cactcgtggt
      181 acacgacacc gacgtcagag cctgcggcga tctgtggcgg ctggcgcaac cccctgccgg
      241 tcagccagcc cagcgctcct cctgcggcca gaccgatgaa gccgcggcgt gacagcagcc
      301 ctcgggcgct ccaccagggc atcggcgctt ctgccttccg ctccttccgc tggaccgatg
      361 tcggcgcggc ccgcgccggc tcgcgccgaa cgcgaaagcg gccatagctc acgagtcggc
      421 cgacgttgag ggcaacgtgc gccacggcca ggccacccat gacgtatccc gccacggtgt
      481 gccaccaaaa gtcgttcagg tcccacaggt cggcgatgac gccggtagcg ccggtgacca
      541 ccacgctgag cagcaggccg acgctcacca gccagtcgac gtcctggcgc agccgccgca
      601 tgcgccccag ggcagcatcc ggcgggccag cccgagcgca ctagctgaag ttcattccgg
      661 ctcgactgga ccgacccact ccgttacccc gaacgagaat gcgagatgcg cgacgccctc
      721 gtcgggactg gcaccgtgcc agtgctcctc gcccggcgcc gcatagagac aggttcccgg
      781 tgcgagctcg acgggcggct cgccgcgact ctgcgcgacg cccttgcctt caaccacgta
      841 caggtactgc ccagtggggt gccggtgcca gtgcgtgcgt ccaccaggac cgaatcggac
      901 caggagggcg ctgctcggcg gatcatcgac cgcgatcagg tcatgccggc tggcgggacc
      961 ggtgaaatgc tgtggatcgg ccgcgttctc ggccgtttcg gaaagccgtg aagtcttcaa
     1021 gagcttctcc tgtgagtggg gctcctactg tatcgagcgg ctacgggcgt gcggcacccg
     1081 cacacgcgtt ccctgttagc cagctgaccc gtcatgccca actgatgacg gctcgcgggt
     1141 ggctgcgaac gtggcacgat tgtgtcctca tgcccaatga ccaggccgtc gaggagaccg
     1201 tgcattaccc caacggcaag accaagtaca ccggcttcct gctggatggc cagatgcacg
     1261 gcgcctggtc ctggtacagg accgatggct ccctgatgcg caccggcgag tttgatcgcg
     1321 gccaacaggt cggcagctgg cggaccttct cccgtgacgg ccgcgtaatg aaagagacga
     1381 gctttcaccg atgaccgagc gccactccta tctccactcc ctcgagtgca gctcgtgtgg
     1441 cgtcgttctc gacgcgaacc agctgcatgg ggtctgcccc gagtgcgggc tggtcctcct
     1501 ggcccgatac gaccttgaga cgctgcgcaa cgacatgccc cagccgtcct tcgaagggcg
     1561 ccggacggac ctgtggcgtt accacgagct gctgcccgtc agcgatcccg cgcacaccga
     1621 tggcctgggc gagggcggca cgccgctgct ccgtctgacc ggcagggcgg ccagccaggt
     1681 cggcatggac ggcggcgagc tgttcgtgaa agaagagggg ctaaacccca ccggctcgtt
     1741 caaggcccgc ggcatggccg ttgccgtgac acgagcggcg gaactggggg tgaccgacgt
     1801 ggccctgccc tcggccggca atgccgccgg tgcggcagcc gcctacgcgg cagccaccgg
     1861 gctgggcgcc cacgtcgcca tgccctccga cgccccgccc gcaaacctgg cagaagccgc
     1921 gacctacggc gccgaggtga tgctggtcga cggcctgatc gatgccgccg gccgcctcat
     1981 ccgcgagctc gccgccgagc acggctggtt cgacctgtcc acgctgcgag agccgtatcg
     2041 ggtggagggc aagaagacga tgggcatcga gctcgccgag gccggcggct ggggtgacga
     2101 ctggtgcccg gacgcgatca tctacccgac cggcggcggc actggcatcg tcggcatgtg
     2161 gaaggggttt gacgagctgg agcagctcgg ctggatcggt ccacgccgtc cgaagatggt
     2221 ggtcgtgcag gccaccggct gtgcgcccat ggtccgtgcc ttcgagaacg gtgcggatcg
     2281 cgcggagcca tggaccgatg cgcacaccat cgcagcgggc atgcgggtgc cgtcggccat
     2341 cggcgactac ctcgtgctgc gcgccatccg cgacagcggc ggaaccgccg tggccctgga
     2401 tgacgagacg atcgtggatg cccagctccg cctggcgcgc gccaccggga tctacccggc
     2461 gccggaaggc gctgccgcct atgccgcggt ggggcggctg cgcgagacgg gattccttgc
     2521 gggccacgag cgagtcatcg tcttcaacac cggcaccggc ctgaagtacc cgccgccagc
     2581 gatggcttga taggcgaagg gatactcggg agagccggga ggtagccccc atgcccgatc
     2641 gctcccgatc cgagcagctg accctcgccg ccttcctcct gctggtcgtc cttgccggcg
     2701 ggaacgcggt cggcatcgat atcgcgtccc aggagctgga cgcattctgg gcggccggca
     2761 cacgattcgc ggctggcggg ctgatcttcg ccatcctgat gggcgtcttc agtgtggcgc
     2821 ttcccaccgg cggtgctttg atcggcgcgc tgctctacgg cgtccttggc ttcggcgcgg
     2881 cgttcgggct ggcgttcgtg gcgattccca tgaccggcgc cggtaccggc cagctgctcc
     2941 tgggcctggt gccgctgctg accgtggtcc tggcacggct ccatgggctg gagcagatcc
     3001 gcctgcagtc ggtcatcggt tcggtcgttg cgctgggagg cgtggccatc ctggctgcag
     3061 accgcatcgc ggccgatatt ccgctgctgg gcatcggcct cgcgttcctg ggggcgctct
     3121 tcctgtccga ggccggggtc atcgtgaagc tcacccctcg ggcccatccg atcgcgacca
     3181 atgccgtggg gatgcttacc ggcgctggct tgctgctgcc agtgtcgctg ctcctcggcg
     3241 agacctggac gctgcccgat caggctgaca cgtgggcggc catgacctac ctggtcgtcg
     3301 ccggctcggt ggcggtgttc gccctgaacg tctacgtgct cggccggtgg ccggcatcgg
     3361 tcgtggccgt cgagttcctg ctcattccgc tggccacgat cccgttctcc gcggtgctca
     3421 cggaagaggg gatcaccccg atcatgctgc tgggaggcgc cgtcatcctc tccggtgttt
     3481 acttcggcgc actcgggccg gagctcggtc gcggacggga ggcgcgagtc gccgacacgt
     3541 aacctgtcag gcgcggcgct tccgcggctt gggctccttg gatggcagcg tgcgggcgta
     3601 ctcggcggcg cgctcgatcc agccgcgcgt ggcatcggca tccgcgaaca cctccgccgg
     3661 gagcgcgacg tactcgcgca tcggcctgcc gggcatcggt tcgaacatgg accagccggc
     3721 atccagccgt tcctgccgct cgtcctccgg gagccggacc atgacggtgt cctggtgcag
     3781 gccggccacc atgtttccgc caacgaacgc cgccggaaag ccgaacatct tccgctgctc
     3841 gatgccctcg atccccccga tcgcctcctc gaagcgctca acatcctccg ggcgcgcctt
     3901 gcgccaggga ttctccgcca tgtggcactc cttcctctcg gcgcggatca gccgcgcagc
     3961 tctcgctcaa gcccgtccag gatcaggtcg agcccgaagt cgaactcgct ggtctccccg
     4021 ggcctgtctg acatatgtac cccgatgtgc tccgcgaggt aaggaaggtc gtcaacggag
     4081 acgcgcgaga tgaagtcctg gaccaggccc ggctgctccc tcgcaacctt gaggtaaggc
     4141 aagacccaga gcgtgaagcc gacgatgtgg ctgtccagcg cgtgataaga gtggtgcgtc
     4201 atgtccgccg agaaccccgc gtgacgcagc cgtcggagaa cgctgttcat ccaccgcagg
     4261 cgggccgcac tgggccctgt cggctgcatc aggagcccgc acgcccactc gtgacgcagg
     4321 aggacgtggt gggccgatat cgccgtccgc cgcagggccg ccttccagtc gccctcctcc
     4381 gagggcagct ccatctcggc gaagacgatg tccagcatgc cggccagcag gtcgtccttg
     4441 ttggcgacgt ggtggtagag cgacatggcc tcgacgccga gcggctgtgc gaggcgacgc
     4501 atgctcagct gctcgatccc ctcctcgtcc gccagctcga tgccggcacg cagcacccgt
     4561 tcccggctga gcgggaggcg ctggcggctg ggtcggccaa tcttggtgga catcggtctt
     4621 gacacactct tacgccgtaa ggatactgtg ctgcacgtct aaccttacat cgtacgaaag
     4681 gaacttgtgg acatctacga ggccctgtac acgacgcgcg ccatgcggcg gctgaagccg
     4741 gatccgatcc cccacgac
//
LOCUS       DATACA010000160         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1869856, whole genome shotgun sequence.
ACCESSION   DATACA010000160 DATACA010000000
VERSION     DATACA010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869856"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..911
                     /locus_tag="VHK28_03295"
     CDS             162..911
                     /locus_tag="VHK28_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX2141292.1"
                     /translation="MTQPALVVDGLWKSFGQLEVLRGMSLTLEEHEVVCLIGASGSGK
                     STLLRCINLTEPIDGGRIVVEGEEITAEGVDVDRIRRRIGIVFQSYNLFPHMSVIDNV
                     TLAPRKVLGRSRRQAEEQADALLERFGLAEKRDDFPDRLSGGQQQRVAIVRALAMDPD
                     LLLLDEVTSALDPELIAEVLNVIRELAGQGMTMLIATHEMGFARDIADRVAFLDGGSV
                     LEQGPPERIFSSPSEPRTREFLQRIIEAGRL"
     gene            complement(930..1535)
                     /locus_tag="VHK28_03300"
     CDS             complement(930..1535)
                     /locus_tag="VHK28_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEX2141293.1"
                     /translation="MKVASRHWPRNGWMPSADVMVIGLVGAALYAVFNWIAWDILIPG
                     TDDVAIRPHYGLLTFFGFAFGPLAGLLTGLIGNAVGDVLGGFNPLSSWWWTVANGLAG
                     MLAGVLSIVLVRSAAEGRALLVMAAIAAVAATMLGFGFIWIELVTQPEMTPRQILLTE
                     YIPVVVGNSLAAAVVTPVLVLAWRPLHEQLAAASMEQEPRG"
     gene            complement(1609..3126)
                     /locus_tag="VHK28_03305"
     CDS             complement(1609..3126)
                     /locus_tag="VHK28_03305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2141294.1"
                     /translation="MAITLQFLGAVGTVTGSQFLLTAGDRRVLVECGMFQGSPEEVSR
                     NRLPFAFEAGELDAVLVTHAHLDHCGRLPALVAAGYHGPIYLTEATADLAEIVLRDAA
                     KLQGEAEARWRRKHPDEAEAHDEAMARAESDAQAEDMPDRLREAEPRGATMVRAALYT
                     EADVEQTLSQFRPVPYGKVLDITNGVRATFHDAGHILGSAIIEVTAEGTTVVFSGDLG
                     RRSTPILRDPTPLSHADYVVVESTYGNREHASHDAAIEELVQTIAEVADDGGVLLVPA
                     FAIGRTQEVVWVLDELVRAGRIPRLPLYLDSPMASQASAVYLRHPDVYDEQTEELLQS
                     GESPLEYPGEQFTNSVEESKAIRHATRPLMVVASSGMLTGGRIMHHLKDFLPDPSCTL
                     LFIGYQARGSLGRHLQSGGRTARIDGDEYPVRCRVRSISGFSAHADEHELTEWLRSFV
                     RAGGSNGTDGRPKRVFLVHGDPESAEPFAERIRGELGLEPHLPRYRETVTLHDGT"
     gene            3171..>3395
                     /locus_tag="VHK28_03310"
     CDS             3171..>3395
                     /locus_tag="VHK28_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2141295.1"
                     /translation="MAFPIPPPIDPMLAKPTPELPAGDGWRYEPKWDGFRAIVFRDGQ
                     EVYLQSRDLKPLDRYFPELADPVRAIGTADA"
BASE COUNT          499 a         1184 c         1197 g          515 t
ORIGIN      
        1 gccacagcgc tggtcttcct actgctcacc atcccgctcg cccggttgac cgattggctg
       61 gttgcccgcg agcgcgaccg gcgcttcgcc gccgcggtcg aggtttccgg ctcccgcgct
      121 cggcgcgggc ggctgctcgg cctgggcggg ggacgggacc gatgacccag ccggccctgg
      181 tggtcgacgg cctgtggaag tcgttcggcc agctggaggt gctgcgcggc atgtcgttga
      241 ccctggagga gcacgaggtg gtgtgcctca tcggtgcctc cggctccgga aagtcgacgc
      301 tgctgcgctg catcaacctg accgagccga tcgacggcgg gcggatcgtg gtcgagggag
      361 aggagatcac cgccgagggc gtggacgtgg accgcatccg acggcggatc ggaatcgttt
      421 tccagtccta caacctgttc ccgcacatgt cggtgatcga caacgtgacc cttgccccgc
      481 gcaaggtgct cggacgctcg cggcgccagg cggaggagca ggccgacgcg ctgctggagc
      541 gattcggact ggcggagaag cgcgacgact tcccggaccg cctctccggc gggcagcagc
      601 agcgggtggc catcgtgcgt gccctggcca tggatcccga tctgctgctg ctcgacgagg
      661 tgaccagcgc cctggacccg gaactcatcg ccgaggtgct caacgtgatc cgcgagctgg
      721 ccgggcaggg aatgaccatg ctgatcgcca cccacgagat gggcttcgcg cgtgacatcg
      781 ccgatcgagt cgcgttcctg gacggcggca gcgtcctgga gcagggtccg ccggagcgca
      841 tcttcagcag tccctcggag ccgcgaaccc gcgagttcct gcagcggatc atcgaggccg
      901 gccgcctcta gccttcgaaa cccctcccgc tagccccgcg gctcctgctc catggaggcc
      961 gccgccagct gctcgtggag ggggcgccag gcgaggacca gcaccggcgt caccaccgcg
     1021 gccgccagcg agttgccgac cacgaccggg atgtactcgg tcagcaggat ctggcgggga
     1081 gtcatctccg gctgggtcac cagctcgatc cagatgaagc cgaagccgag catggtggcc
     1141 gccaccgccg cgatggccgc catgaccagc agcgcgcgcc cctcggccgc ggatcggacc
     1201 aggacgatcg acagaacccc ggccagcatg ccggccagcc cgttggccac cgtccaccac
     1261 cacgagctga gcgggttgaa ccctcccagc acgtctccca ctgcgttgcc gatcagccct
     1321 gtcagcaggc cggccagggg tccgaaggcg aagccgaaga aggtcagcag gccgtagtgc
     1381 gggcggatgg cgacgtcatc ggtccccggg atgaggatgt cccaggcgat ccagttgaac
     1441 accgcgtaca gggcggcacc gacgaggccg atcaccatca catccgcgga cggcatccag
     1501 ccattgcgcg gccagtgtcg gctggcgacc ttcatgacgg gtgatctctc cggcgggctc
     1561 gggcctgatg gataccgata tggatcgtgc ggcgcctccg gccgctcgtc aagtaccgtc
     1621 gtggagggtc acggtctcgc ggtagcgggg caggtgtggc tccagtccga gctctccgcg
     1681 gatgcgctcg gcgaacggct cggcggattc gggatcgccg tggaccagga agacgcgctt
     1741 gggccggcca tccgtcccgt tgctcccccc ggcccgcacg aagctgcgca gccactcggt
     1801 cagctcgtgt tcgtcggcgt gggctgagaa gccggagatg gaccgcaccc ggcagcggac
     1861 cgggtactcg tcgccgtcga tgcgcgccgt gcggccgccg gactggaggt gccgccccag
     1921 gcttccgcgc gcctggtaac cgatgaacag cagggtgcag ctcgggtcgg gcaggaagtc
     1981 tttgaggtgg tgcatgatcc ggccgccagt caacatcccg gagctggcga ccaccatcag
     2041 cgggcgcgtc gcgtggcgaa tggcctttga ctcctcgacg ctgttggtga actgctcgcc
     2101 ggggtactcg agcggcgact cgcccgactg cagcagctcc tcggtctgct cgtcgtagac
     2161 atcggggtgg cgcaggtaga ccgcgctggc ttgcgacgcc atgggcgaat ccaggtagag
     2221 cggcagtcgc gggatgcggc cggcccgtac cagctcgtcc aggacccaga ccacctcctg
     2281 ggtacgaccg atggcgaagg ccggcaccag tagcacgccg ccgtcatcgg ccacctcggc
     2341 gatggtctgc accagctcct cgatggccgc gtcgtgcgac gcgtgctcac gattgccgta
     2401 tgtggactcg acgaccacgt agtcggcgtg gctcagcggt gtcgggtcgc ggaggatcgg
     2461 ggtcgagcgg cgtcccaggt cgccggagaa gacgacggtg gtgccctccg cagtcacctc
     2521 gatgattgcc gagcccagga tgtgccccgc gtcgtggaag gtggcgcgca cgccgttggt
     2581 gatgtccagc accttgccgt agggcaccgg ccggaactgg gagagggtct gctccacgtc
     2641 ggcttcggtg tacagcgcgg cgcgcaccat ggtcgctccc cggggctctg cctcccgcag
     2701 ccggtccggc atgtcctcgg cctgcgcgtc ggactcggcg cgcgccatgg cctcgtcgtg
     2761 ggcctcggct tcgtcggggt gcttacggcg ccagcgcgcc tcggcctcgc cctgcagctt
     2821 ggcggcgtcg cgcaggacga tctcggcgag gtcggcggtg gcctcggtga ggtagatcgg
     2881 gccgtggtag ccggcggcca ccagcgccgg caggcggccg caatggtcga ggtgcgcgtg
     2941 ggtgaccagc accgcgtcca gctcaccggc ctcgaaggcg aacgggaggc ggttgcggct
     3001 cacctcctcc ggcgagccct ggaacatccc gcactcgacc aggacccggc gatccccggc
     3061 ggtcagcagg aactgcgagc cggtcacggt gcccaccgca cccaggaact gcagcgtgat
     3121 tgccatgcgc cccgagtatg gccgatgcgc ccgcgtatca tcgggcggcc atggccttcc
     3181 ccatcccgcc gcccatcgat cccatgctcg ccaagccgac ccccgagctc cccgctggcg
     3241 atggctggcg ctacgagccg aagtgggacg gcttccgggc catcgtcttc cgcgacgggc
     3301 aggaggtcta cctccagtcg cgtgacctca agccgctgga ccgatacttc cccgagctcg
     3361 ccgacccggt gcgcgcgatc ggcaccgccg atgcc
//
LOCUS       DATACA010000161         3940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1548861, whole genome shotgun sequence.
ACCESSION   DATACA010000161 DATACA010000000
VERSION     DATACA010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548861"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VHK28_03315"
     CDS             <1..774
                     /locus_tag="VHK28_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX2141296.1"
                     /translation="EGWLYGLGVLDFAGGTVVHLSSGVAALVAALMIGRRLGLVRHAT
                     EPHDVPMTVLGAGLLWFGWFGFNAGSALGANGLAATAVVTTNTAAAMAALTWVTISWL
                     HRGTPSVVGAAIGAVAGLVAITPAAGFVSPAASILIGFGVGVVCYGASLLRLRSRADD
                     ALDVFAVHGVGGAFGALAVGVFASTAVNAAGRDGALAGNLAQLPLQAFGAGVVAVFSA
                     VMTAGILLVVNLVVRIRVPGEAEEAGLDLAQHGEVAYQS"
     gene            complement(890..1477)
                     /gene="ybeY"
                     /locus_tag="VHK28_03320"
     CDS             complement(890..1477)
                     /gene="ybeY"
                     /locus_tag="VHK28_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX2141297.1"
                     /translation="MPSTVTFHDRIGKEQVEAAIEPMSVDQLHRLLEGAVNEATRITL
                     GGTEVDLELTLCGDAEMTRLNFDHMGERGPTDVLAFPLHEWTIDGRHSHLADDDGAAP
                     PGALLLGDLVVDVDQAVRQASEGGWSVAEEIVLLVVHGTLHLVGHDHAELDEEQEMRG
                     AEHQVLGSLHRRFRDVDWKPGSLFDRAGHAVTSGS"
     gene            complement(1531..3645)
                     /locus_tag="VHK28_03325"
     CDS             complement(1531..3645)
                     /locus_tag="VHK28_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX2141298.1"
                     /translation="MFIPRTLAESEDGSRAAWIRVLVVASVLTAVLWAVLTVNVSASQ
                     VNLEVGDVAPDDISAPRTESFVSESLTDARRQEAAAGVQDIYVPIGPRADIREAQLRQ
                     FDSRVRVVNGVLTRRDAGTLDAEEVVPEINRVAPELSTAQVDQLASMPAGTWERVAEA
                     GRTVLEDALSGEVREDRLADVRAQVRSNITNELAPAERELAGDMAALYVVPNEQYSAE
                     ETEAARQAARDAVDPEVISVQEGQTIVRAGDRIEEVDRETLEALGLTRQQTEVSTLAG
                     NAVIAGVIATLLVSFLWRFEPHIWFRNRSLLLFVLALVLTAVALRLSADRTLWAYVVP
                     SAATVLLAAILLDGAAGAAMAVALAVLAGVMNRDSLEPAVYTLAGGIVAVTAIVRAER
                     LNAFLRAGVLLAATNMAVLLAFNLLGQRDVTAMLQGFGAGAVNAGLSVVLAVGSFAVI
                     GNLFGIMTVFQLLELANPSNRLLRRLLLETPGTYHHSVMVGNLAERAAETIGADPLLA
                     RVAAYYHDIGKMKNPLAFIENQAGARNIHDDLTAEVSARIIASHIRDGIDLAYEHALP
                     VQVIGFIPQHHGTSVMSYFYGKALREVGGQDDMVSKDTYRYPGPKPQSREAAILMLAD
                     GVEASVRSLDDKDEPAIRAMVDRIVDARVEDGQLDDAELTLKNIAQIKEAFVQQLLGM
                     YHSRIKYPDNIVPLEQPRREQA"
     gene            complement(3649..>3940)
                     /locus_tag="VHK28_03330"
     CDS             complement(3649..>3940)
                     /locus_tag="VHK28_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX2141299.1"
                     /translation="EMKQRRESIEQFRAGGREELAAAEEAEAKILAEFLPDPMDAEEL
                     DAVVRETIAATGASGPAGMGKVMGAVMPKVRGRADGKQVSAAVRRNLGCEAG"
BASE COUNT          529 a         1400 c         1372 g          639 t
ORIGIN      
        1 gaaggctggc tctacggcct gggcgtgctc gactttgcgg gcgggacagt ggtgcacctc
       61 tcgagcgggg tggcggccct ggtggcagcg ctgatgatcg gccgccggct gggactggtg
      121 cgccacgcca cagaaccgca cgacgtaccg atgaccgtgc tgggcgccgg gctgctgtgg
      181 ttcggctggt tcggcttcaa cgccggctcg gccctcggcg ccaacggcct ggcggccacg
      241 gccgtggtca ccaccaacac ggctgcggcc atggccgcac tgacctgggt gaccatctcc
      301 tggctgcatc gcggcacccc gtcggtggtg ggcgcggcca tcggtgccgt agccgggctg
      361 gtggccatca cgccggcggc cggcttcgtc tccccggcgg cctcgatcct tatcggcttc
      421 ggcgtgggcg tggtgtgcta cggcgcttcg ctgctgcgcc tccggtcgcg agcggacgac
      481 gccctggacg tcttcgcggt gcacggcgtg ggtggcgcct tcggcgcgct ggcggtcggt
      541 gtcttcgcct cgaccgcggt gaatgccgcc ggtcgcgacg gagcgctggc cgggaacctg
      601 gctcagctgc cgctccaggc cttcggggcc ggggtggtgg cggtgttctc ggcggtgatg
      661 accgccggca tcctgctggt cgtaaacctc gtggtgcgca tccgcgtccc gggggaggcg
      721 gaggaggcag gactcgacct cgcccagcac ggcgaggtcg cgtaccagtc gtagcctcta
      781 tcaaggagga cgtggaccga tggagatcgc cgcactgctg ctcttcggct cgctgctggc
      841 aggctggctg ctggccccgt cggaggtgcg ccgcgacgag ctgagctgat cagctcccgc
      901 tggtcaccgc gtgccccgcg cggtcgaaga gggagccggg cttccagtcg acgtccctga
      961 agcgccggtg cagggaaccc agcacctggt gctcggcgcc ccgcatctcc tgctcctcgt
     1021 ccagctcggc gtggtcgtgg ccgaccaggt gcagcgtgcc gtggacgacc aggagcacga
     1081 tctcctcggc cacgctccag ccgccttcgc tggcctggcg caccgcctgg tcaacgtcca
     1141 ccaccaggtc gccgagcagc agcgcgcccg gtggagcggc gccgtcgtca tcggccaggt
     1201 gggagtggcg cccgtcgatg gtccactcgt ggagggggaa ggcgaggacg tcggtcgggc
     1261 ctcgctcgcc catatggtca aagttcaggc gcgtcatctc cgcgtcgccg cacagggtca
     1321 gctccagatc gacctcggta ccgcccagcg tgatgcgggt tgcctcattg actgcgccct
     1381 ccagcaggcg gtgcagctga tccaccgaca tgggctcgat ggcagcttcc acctgctcct
     1441 tgccgatgcg gtcgtggaag gtgacggtgg agggcatcgc acacccatcc taggcgaacc
     1501 ccccgcccgc ctcaaggccc ggcgcgggtc tcaggcctgc tcgcggcgcg gctgctccag
     1561 cggcacgatg ttgtccgggt acttgatgcg gctgtggtac atgcccagca gctgctggac
     1621 gaacgcctcc ttgatctggg cgatgttctt gagcgtgagc tcggcatcgt ccagctgtcc
     1681 gtcctcgacc ctggcatcca cgatgcggtc caccatggcc cggatggccg gctcgtcctt
     1741 gtcgtccagg ctgcgcaccg atgcctcgac gccgtctgcc agcatcagga tggccgcctc
     1801 ccggctctgc ggcttggggc ccgggtagcg gtaggtgtcc ttgctgacca tgtcgtcctg
     1861 cccgcccacc tcgcgaagcg ccttaccgta gaagtagctc atcacgctgg ttccgtggtg
     1921 ctgcgggatg aagccgatga cctgcacagg caatgcgtgc tcgtaggcca ggtcgatgcc
     1981 gtcgcggatg tggctggcga tgattcgggc gctcacctcg gcggtcaggt cgtcgtggat
     2041 gttgcgggcg ccggcctggt tctcgatgaa ggccagcggg tttttcatct tgccgatgtc
     2101 gtggtagtag gccgccacgc gggccagaag cgggtcggca ccgatcgtct ccgcggcgcg
     2161 ctctgccagg ttgccgacca tcacgctgtg gtggtacgtg ccgggcgtct cgagcagcag
     2221 cctccgcagc agccggttgg acgggttggc gagctccagc agctggaaga cggtcatgat
     2281 cccaaacagg ttgccgatca ccgcaaagga cccgaccgcg aggacgaccg acaggccggc
     2341 attcaccgcg ccggcgccga agccctgcag catggctgtc acgtcgcgct ggccgaggag
     2401 gttgaacgcg agcaggacgg ccatgttggt ggccgccagc agcaccccgg cgcgcaggaa
     2461 ggcgttgaga cgctccgctc ggacgatggc cgtcaccgcc acgatgcctc ccgccagggt
     2521 gtacaccgcc ggctccagcg agtcgcggtt catgacgcct gccagcacgg ccagcgccac
     2581 ggccatggcc gctcccgccg cgccgtcgag caggatggca gccagcagca cggtggccgc
     2641 gctggggacg acgtaggccc acagggtgcg gtcagccgag agacgcagtg ccacggcggt
     2701 caggaccagg gccagcacga agagcagcag cgagcggttg cggaaccaga tgtgcggctc
     2761 gaaccgccac aggaaggaga ccagcagggt ggcgatcacg ccggcgatca cggcgttgcc
     2821 ggccagggtc gagacctccg tctgctgacg ggtcagaccc agcgcctcga gcgtctcgcg
     2881 gtcgacctcc tcgatccggt cgccggcacg aacgatggtc tggccttcct gcacggagat
     2941 gacctccgga tccaccgcgt cgcgtgcggc ctggcgggcc gcctccgtct cctccgccga
     3001 gtactgctcg ttggggacca cgtacagcgc ggccatgtcc ccggccaact cccgctcggc
     3061 cggcgccagc tcgttggtga tattcgagcg cacctgggcc cgcacgtcgg ccagccggtc
     3121 ctcccggacc tcgccggaga gcgcgtcttc cagcacggtg cgccccgcct ccgcgacccg
     3181 ctcccaggtg ccggccggca tgctggccag ctggtcgacc tgtgcggtgc tgagctcggg
     3241 ggcgacccgg ttgatctccg gcacgacctc ttcggcgtcg agcgtccccg cgtcgcgccg
     3301 ggtcagcacg ccgttcacca cccgaacgcg cgaatcgaac tggcgcagct gggcctcgcg
     3361 gatatcggct cgcggcccga tggggacata gatgtcctgg accccggccg cggcctcctg
     3421 gcgacgtgcg tcggtcaggg actccgacac gaagctctcc gtgcgcgggg cgctgatgtc
     3481 gtccggcgct acgtcgccga cctccaggtt cacctggctg gctgagacgt tcaccgtcag
     3541 caccgcccat agcaccgcgg tgagcacgct cgccacgacc agtacccgga tccaggccgc
     3601 gcggctgccg tcctccgact cggcgagggt acgggggatg aacacggcct agcccgcctc
     3661 gcagcccagg ttgcgccgca cagcggccga aacctgcttg ccgtcggccc ggcctcgaac
     3721 ctttggcatc acggcgccca tcaccttgcc catccctgcc ggacctgacg cgccggtggc
     3781 cgcgatcgtt tcgcgcacca cggcatccag ctcttcggcg tccatcggat ccgggaggaa
     3841 ctcggccagg atcttcgcct ccgcctcctc ggcggccgcc agctcctcgc ggccaccggc
     3901 gcggaactgc tcgatcgact cgcggcgctg tttcatctcg
//
LOCUS       DATACA010000162         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_183827, whole genome shotgun sequence.
ACCESSION   DATACA010000162 DATACA010000000
VERSION     DATACA010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183827"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VHK28_03335"
     CDS             <1..1274
                     /locus_tag="VHK28_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2141300.1"
                     /translation="EWYVGLGTGTNPEQIPVQEEQVELFNEEHPDIHLTLTVVDSEVA
                     EQDLATRLPTNPPDIIGPIGIRGLQGYGDSLLDLTEYIESAGVDRSSIPDALWDVYQL
                     DGKQIGIPMAVYPSFIFYNKALFDEAGLAYPPHEVGEQYEGQDWTWETVRELAMQLTV
                     DTNGNDASSADFDPSAIEVWGLDAQFTNNDPRAWSTIFGGSGSVVADDGTTAQWPDNW
                     RDGLQFFYDGMWVDHFIPTKPELDSIEPNGNSFQTGRIAMDVIHQWYTCCIYPPEGEP
                     PVSDWDIAVLPEGPDGNITSKLHADTVGILDSTENPEAAFVVLDWIRQNPALLETWGA
                     LPAPTDQRDAFFAALDERFAPLEVDWNVSTLMLEYPDVPSHEAFMPNFTEANAANGEF
                     GSSLWTTADLDLDSEIDAHVERLQGIFEEAD"
     gene            1361..2320
                     /locus_tag="VHK28_03340"
     CDS             1361..2320
                     /locus_tag="VHK28_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2141301.1"
                     /translation="METTTESAAALTAAPPRRGRLARLGDREGMTGLIFVSPWIIGFL
                     AFSAIPMAASLVLSLTDFDPRRADELRFIGLQNYTRMLSDPALIKSAVVTLRFALIVV
                     PITLAFALAVALLVNHKLLAGRNVFRTLFYMPLQIPIVASTVVWIGVLNVSTGWLNLG
                     LEAVGGPSIDWLNNPFWVHPALALMGLWGIGNMMLIFLAGLQSVPSELYDAAKVDGAG
                     PWATFRKVTLPMISPVLFYNLIIVLIATFQYFTQAYVITNGRGDPGGETLFFNLNLFR
                     EAFQFYNMGYASALAWVLFAVVLGLTIVLFRTAGHWVYEGGER"
     gene            2317..3399
                     /locus_tag="VHK28_03345"
     CDS             2317..3399
                     /locus_tag="VHK28_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2141302.1"
                     /translation="MTAVARPVDRSISAGSPMRGQMRHFLAVSGFTLFASILVSVFLM
                     PLGYMAATAVKSETQTSTPGAPIWPAAPETFSYEGREYPVFQVPTEDGVRSWALIEPH
                     REDAVFIDPENPEAGPITWEGRWRTLEQSWTFSLDWSSFSRAWEILNFPRMFFNTFMI
                     AALSTVGAVLSAICVAYGFSRFRFPGRNGLFLLMLATIILPFQVTLIPTYAVYFALGW
                     VGTWLPLIIPHFFANAYNVFLLRQYFLTIPRDLDEAAMIDGAGPFRILRSVIIPQAIP
                     AIVAVTLFHFFWAWNEYFLPLVYLQGNPDLQPLSVGLGRFNSLYSGEPTVIQAAALMA
                     MALPVVVFFFAQRAFMRGVVFGGVEK"
BASE COUNT          524 a         1184 c         1091 g          605 t
ORIGIN      
        1 tcgagtggta cgtcgggctc ggaaccggca ccaacccgga gcagatcccg gtgcaggaag
       61 agcaggtgga gctcttcaac gaggagcatc ccgacatcca cctgacgctg acggtggtag
      121 acagcgaggt ggccgagcag gacctggcca cgcggttgcc gaccaacccg ccggacatca
      181 tcggtcccat cggcatccgc ggcctgcagg gctacggcga tagcctgctg gacctgaccg
      241 agtacatcga gtcggccggt gtcgatcggt cctccatccc ggacgccctg tgggacgtgt
      301 accagctgga tggcaagcag atcggcatcc cgatggccgt ctacccgtcg ttcatcttct
      361 ataacaaggc cctcttcgac gaggccgggc tggcctatcc accgcacgag gtcggtgagc
      421 agtacgaggg ccaggactgg acgtgggaga ccgtccgcga actcgccatg cagctcacgg
      481 tcgacaccaa cggcaacgac gcgagcagcg cggacttcga cccctcggcc atcgaggtct
      541 ggggtctgga cgcgcagttc acgaacaacg acccgcgggc ctggtccacc atcttcggtg
      601 gctcgggatc ggtggtcgcc gacgacggga cgacggccca gtggccggac aactggcgcg
      661 acggcctgca gttcttctac gacgggatgt gggtcgacca cttcatcccg accaagccgg
      721 agctcgactc gatcgagccc aacggcaaca gcttccagac cggccggatc gcgatggacg
      781 tgatccacca gtggtacacc tgctgcatct acccgcccga gggcgagccg ccggtcagcg
      841 actgggatat cgccgtcctg ccagagggcc cggacgggaa catcacctcg aagctgcacg
      901 ccgacacggt cggcatcctg gacagcaccg agaaccccga ggcggcgttc gtggtgctcg
      961 actggatccg ccagaacccc gctctgctgg agacgtgggg cgccctgccg gcgccgaccg
     1021 accagcggga cgcgttcttc gcggcgctgg atgagcggtt cgcgccgctg gaggtggact
     1081 ggaacgtctc taccctgatg ctcgagtacc cggacgtgcc gagccacgag gcgttcatgc
     1141 cgaacttcac cgaggccaat gcggccaatg gcgagttcgg gtcctcgctg tggaccaccg
     1201 ccgacctcga cctcgattcg gagatcgacg cccacgtgga gcggctgcag ggcatcttcg
     1261 aggaagccga ctagcagccc cgagcagtcg ggggcggcct ccgggccgtc cccggcgcat
     1321 cattggcgaa ccatcacagc gagggtgagg tgaggtgagg gtggagacca cgaccgaatc
     1381 cgcggccgcg ctgaccgccg cgccgcctcg gcgcgggcgt ctcgcccggc tgggggatcg
     1441 ggagggcatg accgggctca tcttcgtctc accctggatc atcggcttcc tcgccttttc
     1501 ggcgatcccg atggccgcct cgctggtgct gtcactcacc gacttcgacc cgcgccgggc
     1561 cgacgagctg cgcttcatcg gcctccagaa ctacacccgc atgctcagcg acccggcgct
     1621 gatcaagtcg gcggtcgtga ccctgcgttt cgcgttgatc gtggtgccga tcaccctggc
     1681 cttcgccctg gccgtggcac tgttggtcaa ccacaagctg ctggccgggc gcaacgtctt
     1741 tcgcaccctg ttctacatgc cgctgcagat ccccatcgtg gccagcacgg tggtctggat
     1801 cggcgtgctc aacgtctcga ccggttggct caacctgggg ctcgaggcgg tcggcgggcc
     1861 gagcatcgac tggctgaaca acccgttctg ggtgcatccc gccctggccc tgatgggcct
     1921 gtggggcatc gggaacatga tgctcatctt cctggcgggg ctgcagagcg tcccctccga
     1981 gctgtacgac gcggccaagg tcgacggcgc cgggccgtgg gcaaccttcc gcaaggtcac
     2041 cctgccgatg atctcgccgg tcctgttcta caacctgatc atcgtgctga tcgccacgtt
     2101 ccagtacttc acccaggcct atgtcatcac caacgggcgc ggtgatccgg gcggcgagac
     2161 gctcttcttc aacctcaacc tgttccgcga ggcgttccag ttctacaaca tgggctacgc
     2221 atcggccctg gcctgggtgc tgttcgcggt ggtcctgggc ctcaccatcg tgctgttccg
     2281 gaccgcgggc cactgggtct acgagggcgg cgagcgatga ccgccgtcgc gcgcccggtc
     2341 gaccgctcga tctccgccgg gtcgcccatg cgcgggcaga tgcggcactt cctggccgtc
     2401 tcgggcttca cactgttcgc gagcatcctg gtatcggtct tcctcatgcc gctggggtac
     2461 atggcggcga ccgcggtcaa gagcgagacc cagaccagca cgcccggtgc tccgatctgg
     2521 cccgccgccc ccgagacgtt cagctacgag ggccgggagt acccggtctt ccaggtcccc
     2581 accgaggacg gcgtgcggtc gtgggccctg attgagccgc accgtgagga cgccgtcttc
     2641 atcgaccccg agaacccgga ggccggaccg atcacctggg aaggccgatg gcggacgctg
     2701 gagcagtcct ggaccttctc cctggactgg agcagcttca gccgggcgtg ggagatcctc
     2761 aacttcccgc gcatgttctt caacaccttc atgatcgcgg cgctctccac ggttggcgcg
     2821 gtgctgtccg cgatctgcgt ggcctacgga ttcagccgat tccggttccc cggtcgcaac
     2881 ggtctcttcc tgctgatgct ggccaccatc atcctgccct tccaggtcac cctgatcccg
     2941 acatacgccg tgtacttcgc gctcggctgg gtggggacgt ggctgccgct gatcatcccg
     3001 cacttcttcg ccaacgccta caacgtcttc ctgctgcgcc agtacttcct caccattccg
     3061 cgtgacctgg acgaggcggc catgatcgac ggcgccggac cgttccgcat cctgcgctcg
     3121 gtgatcatcc cgcaggccat cccggccatc gtggccgtca cgctgttcca cttcttctgg
     3181 gcctggaacg agtacttcct gccgctggtc tacctgcagg gcaatcccga cctccagccg
     3241 ctgtcggtgg gcctcggtcg cttcaactcg ctgtactccg gggagccgac cgtcatccag
     3301 gcagcggcgc tgatggccat ggcgctgccg gtcgtcgtct tcttcttcgc gcagcgcgcc
     3361 ttcatgcgcg gggtggtctt cggcggggtc gagaagtgag cggc
//
LOCUS       DATACA010000163         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2593047, whole genome shotgun sequence.
ACCESSION   DATACA010000163 DATACA010000000
VERSION     DATACA010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2593047"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1373)
                     /locus_tag="VHK28_03350"
     CDS             complement(360..1373)
                     /locus_tag="VHK28_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX2141303.1"
                     /translation="MPEGFTFLLLTVLALAIIFDYINGFHDTANAIATSVSTRALKPH
                     WAIGMSAVANFLGALTGTEVAHTVGSGLIDPRFEAGASGQVVIAAALVGAIAWNLLTW
                     RLGIPSSSSHALIGGLLGAVGAAAGLGAWQMDGIVNRVLVPLVGSPLAGFAIGLMLMV
                     LIFNLFRRFNPHFMNDLFRRLQVISAAYMAFSHGSNDAQKTMGIMTLALVTAGVIPEF
                     EVPLWVIISAATAISLGTAAGGWRIIRTMGTKVVKLDPVHGFAAETTAATIIYGASQL
                     GMPVSTTHVISSAIMGTGASDRFNAVRWAVARNIGIAWVLTLPAAGLTAAAAYLVLRP
                     LLV"
     gene            complement(1366..1983)
                     /locus_tag="VHK28_03355"
     CDS             complement(1366..1983)
                     /locus_tag="VHK28_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2141304.1"
                     /translation="MRLPLIPREEKFFDMFVEDARNVLGAARLLEEFFRNYGERERIA
                     SQLRDAEHTGDQLSHDIGHKLEHTFVTPFDREDIHALISRLDDILDLIEEVADTCILY
                     GIDQPTEPARQQSEIIVRQCEQIERALGKLRGFKGLEDYWIEVHRLENEGDRIARKAM
                     AELFTNGSQPLDVIKWKDIYALAEEAIDACEDAANVIERIVVKHA"
     gene            2060..2242
                     /locus_tag="VHK28_03360"
     CDS             2060..2242
                     /locus_tag="VHK28_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141305.1"
                     /translation="MFQLEQLTLMHRADGEWHQLSPAEPTPDDYDLERRVLRGGRLFR
                     CSRCEIEVMVSPGEDS"
     gene            2254..>3615
                     /locus_tag="VHK28_03365"
     CDS             2254..>3615
                     /locus_tag="VHK28_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141306.1"
                     /translation="MAADSPARRPAPLRASALAAALVLVAPLLASCVPAARPGTLPTT
                     LVMEDHGGQPIPVQNGIPVPTFDAQPRPRITLSGPWRVERLRLRSGLSLTDRRESLPD
                     ILEEAAGRQRTDYDDGDWETLQVPGSLNPPPLGETGGWYRRTFFVPSGWAGTVTTLKF
                     GAVNYLADVWINGEHVGYHEGGYTPFAFDVSTFLRPGAANQIAVRVDNPPWGTRNDIV
                     PWGLADWWNYGGITQPVWIESTPPVHLVRADVVPHLDGVDISVTLRHAEAVVSEEPTG
                     SPSPQGEEPTPAASSVRPEEAEDQGEPVVRVEILGAEVRPDNLGDPQALSLADTDARA
                     VVDDELELPEVDPGAIATIETGFLLGDPERWSPAQPALYVLHVALAGEGVAADDLWTT
                     FGMRHVAVDPQQALIRLNATPVAFTGVGLHDEVIVADPSGAVELLSASRVHSVEPLLE
                     QLER"
BASE COUNT          568 a         1209 c         1300 g          538 t
ORIGIN      
        1 caacgacgac atccgccgcc tggtccttgg cgtctgacgc gtgcgggtcg tccccggcgc
       61 ggcgccgata cagcgggcaa accccagcgc gaagcgacaa gttcttcgcc cctcacgctc
      121 acggctgccc gatagaacgg cagaaccgca cccgagcggc ccgtgcgggt gaagagccgc
      181 cgctagaaca ggcagcccac gcggccgcgc atcgctgaga agctgccgcg tgggtgcggc
      241 cgggcggcaa ggcgggcggt cgccgagctc cctggacgtt atttcggacg ggggcactct
      301 ccgcgtgtca gatcgtcgac agatcgggca atggcacgca cgaggcgcga agaacgcgct
      361 cagacgagga gcggtcgcag aaccaaatag gcagctgcgg cggtcaggcc cgcggcgggc
      421 agggtcagga cccaggcgat gccgatgttg cgtgcgaccg cccagcgcac cgcgttgaag
      481 cggtcggagg cgccggtgcc catgatggcg ctggagatga cgtgggtggt gctgaccggc
      541 atgcccagct gggacgcgcc gtagatgatg gtggcggccg tcgtctccgc cgcgaagcca
      601 tggaccgggt ccagcttcac gaccttggtg cccatggtgc ggatgatccg ccagccgccg
      661 gccgcggttc ccagcgagat ggcggtggcg gccgagatga tgacccacag cggcacctcg
      721 aactcgggga tgaccccggc ggtgaccagg gccagggtca tgatccccat cgtcttctgg
      781 gcgtcattgc tgccgtggct gaaggccatg taggcggccg agatcacctg caggcggcgg
      841 aacaggtcgt tcatgaagtg cggattgaag cgccggaaca ggttgaagat caggaccatc
      901 agcatcaggc cgatggcgaa gccggccagc ggggagccga ccagcggcac cagcacccgg
      961 ttcacgatgc cgtccatctg ccatgcgccg aggccggccg ccgcacccac cgcacccagc
     1021 agccccccga tcagcgcgtg gctgctcgac gaggggatcc ccagccgcca ggtgagcaga
     1081 ttccaggcga tggcgccgac cagggctgcg gcgatcacca cctggcccga ggcgccggcc
     1141 tcgaagcgcg gatcgatcag gccggagccg accgtgtgcg ccacctccgt gccggtcagc
     1201 gcgcccagga agttggcgac cgcgctcatg ccgatggccc agtgcggctt cagcgcgcgg
     1261 gtgctgacac tggtggcgat ggcgttggcc gtgtcgtgga agccgttgat gtagtcgaag
     1321 atgatggcga gggcgaggac cgtgagcagg aggaaggtga acccttcagg catgcttgac
     1381 gacgatgcgc tcgatcacgt tggcggcatc ctcacaggcg tcgatcgcct cctcggccaa
     1441 tgcgtagatg tccttccact tgatgacgtc cagcggctgg ctgccgttgg tgaacagctc
     1501 ggccatggcc ttgcgcgcga tgcggtcccc ctcgttctcg aggcggtgca cctcgatcca
     1561 gtagtcctcc agccccttga agccgcgcag cttgccgagc gcccgctcga tctgctcgca
     1621 ctggcgcacg atgatctccg attgctgcct ggcgggctcg gtcggctggt cgatgccgta
     1681 caggatgcag gtgtcggcga cttcctcgat caggtccagg atgtcgtcca ggcggctgat
     1741 cagggcgtgg atgtcctctc ggtcgaaggg ggtgacgaag gtgtgctcca gcttgtgacc
     1801 gatgtcgtgg ctgagctggt cgccggtgtg ctcggcgtcg cgcagctggc tggcgatccg
     1861 ctcccgctca ccgtagttgc ggaagaactc ctccagcagc ctggcggcgc cgaggacgtt
     1921 gcgagcgtct tcgacgaaca tgtcgaagaa cttctcttct cgagggatga gtggcagacg
     1981 catcggtcgg gagcatagcg ccgattgacc gatggggcac actgcttgcg ggagaacccg
     2041 caggcgaaag agaggaccga tgtttcagct cgagcagctg acgctcatgc accgcgcgga
     2101 cggtgagtgg caccagctct ccccggccga gccgacgccg gacgactacg acctggaacg
     2161 ccgcgtcctg cgtggtgggc ggctcttccg ctgctcgcgc tgcgaaatcg aggtgatggt
     2221 ctctccgggc gaggacagct gaccggtcgg gcgatggcgg cggacagccc agctcggcgc
     2281 ccggcgccgc tgcgcgcgag cgccctcgcc gcagccctgg tgctggttgc gccactgctc
     2341 gcctcctgcg ttccggccgc gcgccccggc acgctgccga cgacgctggt catggaggac
     2401 cacggcggac agccgatccc ggtacagaac gggatccctg tgccgacctt cgacgcccag
     2461 ccgcgccccc gcatcaccct cagcggaccg tggcgcgtcg agcgcctccg cctgcgctca
     2521 gggctctcgc tgacggatcg ccgtgagtcg ttgccggaca tcctggagga ggcggccggc
     2581 cgccagcgga ccgactacga tgacggtgac tgggagacgc tgcaggttcc aggctcgctg
     2641 aacccgccgc cactcggcga gaccgggggc tggtaccggc gcacgttctt cgtgcccagc
     2701 gggtgggccg gcacggtgac caccctcaag ttcggcgcgg tgaactacct tgccgacgtg
     2761 tggatcaacg gcgagcacgt gggctaccac gagggtggtt acactccgtt cgccttcgac
     2821 gtgtcgacct tcctgcgccc cggcgccgcc aaccagatcg cggtgcgggt cgataacccg
     2881 ccgtggggca cgcggaacga catcgtcccc tggggcctgg ccgactggtg gaactacggt
     2941 ggcatcacgc agccggtgtg gatcgagtcc acgccaccgg tgcacctggt gcgagccgac
     3001 gtggtgccgc acctcgacgg cgtggacatc agcgtcaccc tgcgccacgc cgaagcggtg
     3061 gtgagcgagg agccaaccgg cagcccatcg ccgcagggtg aggagccgac gccggcggcg
     3121 tcatcggtcc ggccggagga agcggaggac cagggggaac cggtcgtccg ggtggagatc
     3181 ctgggcgccg aggtcaggcc cgacaacctg ggcgatccgc aggccctgtc gctggcggac
     3241 accgacgcgc gtgccgtggt cgacgacgag ctcgagctgc cggaggtcga cccgggcgcg
     3301 atcgcgacca ttgagaccgg gttcctgctt ggcgatcccg agcgctggag cccggcgcag
     3361 ccggcgctct acgtgctgca cgtggcgctg gccggggagg gcgtggcggc ggacgacctg
     3421 tggaccacat tcggcatgcg ccacgtggcg gtcgacccgc agcaggcact catccggctc
     3481 aacgccacgc cggtggcctt caccggcgtc gggctgcacg acgaggtgat cgtcgccgac
     3541 ccatcgggcg cggtcgagct gctgagcgcc agccgagtgc actcggtcga gccgctgctg
     3601 gagcagcttg aacgc
//
LOCUS       DATACA010000164         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2031584, whole genome shotgun sequence.
ACCESSION   DATACA010000164 DATACA010000000
VERSION     DATACA010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031584"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1901
                     /locus_tag="VHK28_03370"
     CDS             222..1901
                     /locus_tag="VHK28_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX2141307.1"
                     /translation="MKNALRRWIATSVLLAVSLAACDGDGGTDPQPLTAPTGITGQAG
                     SQQVTITWTAVQDADAYTVQRATGAGEFADVGTTQQASYTDSGLQASTTYRYRVFATR
                     GTQTGPAAEVSITTSNLPVVLIGADITANRTFFRDSVYRLTNFIHVGNGARLTIQSGT
                     RIEGEQGSALFILRGASIEAIGTAAQPIVFTSSRAVGERQPGDWGGVIIIGGGQISRT
                     GDIILEGTGTNNTSNDTIFYSGGGAAGDAGSSGRLSYVRIEFAGFGPAPNQELNSLTL
                     AAVGSGTTVDHVQTLAGLDDSFEWFGGTVDAKYLVSYESGDDHFDASEGYRGRVQFAI
                     AYQDTVLPPRAFAGNLSSDPQGIENDGCDAQTGTCTFNSAPLTTPMFANFTVVGTGPG
                     VVPSGGGIGMLLRRGVGGYYVNGILARWPQAAISIRDQATWDRVAAGNLLVRNLLVAE
                     SGPTFQTGSGRFPVAADSAATKTAWSIQNAAGATGTLFTGFPARPAAPTTATLDWTPP
                     AGSPAATGGTGAFTGTLATVGGTFVTGTDYRGAASPTDDKWWQGWTSYARR"
BASE COUNT          328 a          767 c          704 g          295 t
ORIGIN      
        1 agagaggcag agcagcggaa ggacgatctt cggtcttctg ctgctctgcc tctctgttct
       61 ctgcgtgagt gcagttccgt ccgtgcagcg cgtgatgctt tcttccgcac gtcgtgacaa
      121 tgccgatgca gagccgtgcg ccggtgatga cgacgcctcg cgaggttgat gcagcgtgtg
      181 tgcgcacgcg catcgactga acacccacac tcgggatcga gatgaagaac gcactccggc
      241 gatggatcgc cacgtccgtg ctgctggccg tgtcgctggc cgcctgcgac ggggacggcg
      301 gcacggaccc ccagccgctc accgcgccca cgggcattac cggccaggcg gggagccagc
      361 aggtcaccat cacctggacg gcggtgcagg acgccgacgc ctacaccgtg cagcgcgcca
      421 ccggtgccgg cgagttcgcg gacgtcggca ccacgcagca ggccagctac acggattccg
      481 ggctgcaggc cagcaccacc taccggtaca gggtgttcgc cacccgcggc acccagaccg
      541 gcccggcggc ggaagtcagc atcaccacca gcaaccttcc cgtggtgctc atcggcgccg
      601 acatcaccgc caaccgcacc ttctttcgcg actccgtcta ccgcctgacg aacttcatcc
      661 acgtcggcaa cggggcccgg ctcaccatcc agtccggcac gcgcatcgag ggcgagcagg
      721 gctcggcgct cttcatcctg cgcggcgcca gcatcgaggc catcggcacg gcggcgcagc
      781 ccatcgtctt cacctcgtcg cgcgcggtgg gcgagcggca gccgggtgac tggggcgggg
      841 tgatcatcat cggcggcggg cagatcagcc gcaccggcga catcatcctc gaaggcacgg
      901 ggaccaacaa cacgtccaac gacaccatct tctactcggg cggcggcgcg gccggcgacg
      961 cggggagcag cgggcggctg agctacgtgc gcatcgagtt cgccggcttt ggccccgcgc
     1021 ccaaccagga gctcaacagc cttacgctgg cggccgtggg cagcggcacc accgtggacc
     1081 acgtgcagac gctggccggc ctggacgaca gcttcgagtg gttcggcggg acggtggacg
     1141 cgaagtacct ggtgtcgtac gagtcgggtg acgaccactt cgacgcgtcg gagggctatc
     1201 gcggccgcgt gcagttcgcc atcgcctacc aggacacggt gctgccgccg cgcgccttcg
     1261 cgggcaacct gtccagcgac ccgcagggga tcgagaacga cggctgcgac gcgcagaccg
     1321 gcacctgcac cttcaactcg gctccgctca ccacgcccat gttcgccaac ttcaccgtcg
     1381 tcggcaccgg gccgggggtg gtgccgtcgg gcggcggcat cggcatgctg ctgcggcgcg
     1441 gcgtgggggg ctactacgtg aacggcatcc tggcgcgctg gccgcaggcc gccatctcca
     1501 tccgcgacca ggccacgtgg gaccgcgtgg cggccggcaa cctgctcgtc cgcaacctgc
     1561 tcgtcgcgga gagcggcccc accttccaga cggggagcgg ccgcttcccg gtggcggcgg
     1621 acagcgcggc gacaaagacg gcgtggagca tccagaacgc cgccggcgcc acgggcacgc
     1681 tcttcaccgg cttcccggcg cgccccgcgg cgcccaccac ggccacgctg gactggaccc
     1741 ctcccgcggg ctcccccgcg gccaccgggg gcaccggcgc cttcaccggc accctggcca
     1801 cggtcggcgg caccttcgtg accggcaccg actaccgcgg cgccgcgtcg cccaccgatg
     1861 acaagtggtg gcagggctgg acaagctacg cccgccggta aggaaagacc acggaagcaa
     1921 tcgatgattt cctgagggag gcgccgccgc gatccatccg ttcgcgtgga gccgtggcgc
     1981 ctccctcagg aaatcatcga ttgcttccgt ggtctttcct taccggcggg cgtagcttgt
     2041 ccagccctgc caccacttgt catcggtggg cgacgcggca ccgactaccg cggc
//
LOCUS       DATACA010000165         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2272231, whole genome shotgun sequence.
ACCESSION   DATACA010000165 DATACA010000000
VERSION     DATACA010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272231"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..650)
                     /locus_tag="VHK28_03375"
     CDS             complement(96..650)
                     /locus_tag="VHK28_03375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX2141308.1"
                     /translation="MRTRRRSLLWLTLIAGVGVVLAALVTYPLLRGGATGTEGSLVGG
                     PAPEIEARDLDGRTWSLALDGEGRVTWVNFWATNCEPCRTEMPAMQRLADAYGERLLI
                     LGVDWGESAESVAGFVERYQLEYPILLDPTLDNYYRWAGTDGLPRHYFVDAAGTVVRQ
                     VIGPLEPAAMAGVLDELIGPIGLR"
     gene            complement(655..900)
                     /locus_tag="VHK28_03380"
     CDS             complement(655..900)
                     /locus_tag="VHK28_03380"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX2141309.1"
                     /translation="MSSRQGQITLYERDGCHLCEEARVLMDEMIGPDGYQRVDIDRDD
                     ELVVRYGFRVPVVAVDGVDRLEAPMTAPDLRALLKDT"
     gene            1146..1221
                     /locus_tag="VHK28_03385"
     tRNA            1146..1221
                     /locus_tag="VHK28_03385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Arg,seq:cct)
     gene            complement(1372..2685)
                     /locus_tag="VHK28_03390"
     CDS             complement(1372..2685)
                     /locus_tag="VHK28_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX2141310.1"
                     /translation="MAPFDRLVPPVARMPGFGIELEAYELLVVVVGAALLIGAVVLSV
                     QKGRPFSAAIIYLGIGLAVGIVLDVSETVWFDPTLDPVVFTHVAEFAVIVSLFGAGVK
                     LDRPLTWRAWRSTVMLLLVAMPLTIAGVAAVGYAALGLSIPGAVLLGAALAPTDPVLA
                     DDVQVAGPGDEEPEDEARFAITSEAGLNDGLAFPFVMLALFGAQESFQVASTWLWEWL
                     AADVLYAVAVGIAGGAAAGVGIAALSYWVAGRGWLSKPFDGYVALASILVVYGAVEIV
                     EGYGFLAVFAAGVAFRRYEMHHDYNQRLHEFTLTVEKLAEMAVMLMLRTVLPLADIGE
                     LLLPLLAVTAGLLFLVRPMAVALALLPTRTRRNERAFIGWFGIRGIGSVYYLGFALGE
                     VGTPDARPLFAAISVAIAASVVLHGISSGYLTRRLLGRAAASDQG"
     gene            2804..3514
                     /locus_tag="VHK28_03395"
     CDS             2804..3514
                     /locus_tag="VHK28_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX2141311.1"
                     /translation="MDRRGAALVAEAVGTFLFVFVGAGSVVLATHAGDAGPGLVGIAL
                     AHGLALAVLISAFGAISGGHFNPAVTVAVRLAGRIEWGNAVGYVIAQLLGAAVAAFAL
                     RAVLPESSWEPSGIGSPALGQGIGIGAGIAIEAVLTVLLVLAVFGTAIDARAPRLGGM
                     AIGLAVTADVLMGGPLTGAAMNPARWFGPAVASGQLDNWYVWWVGPLLGGIVAALIYR
                     YALEGEAEPEVTPATPEG"
BASE COUNT          582 a         1348 c         1285 g          531 t
ORIGIN      
        1 gccgaggtgg aggtggaggc catggatgcc ggcgagtact acttcgactg caccattcac
       61 cccaacatga acggcacgct gtacgtggtc gaggcctaac gcaatcctat cggtccgatc
      121 agctcgtcga ggacgccagc catcgcggcc ggctcgagcg gaccgatcac ctgccgcacc
      181 acggtgcctg ccgcgtccac gaagtagtgg cgcggcagcc cgtcggtgcc ggcccatcgg
      241 tagtagttgt ccagcgtggg gtccagcagg atcgggtact cgagctggta gcgctccacg
      301 aagccggcca ccgactcggc ggactcgccc cagtcaaccc ccaggatcag cagccgctcc
      361 ccgtaggcgt cggccaggcg ctgcatggcg ggcatctccg tccggcacgg ttcgcagttg
      421 gtcgcccaga agttcaccca cgtgacccgg ccctcgccgt cgagcgcgag gctccacgtg
      481 cggccgtcca ggtcccgcgc ctcgatctcc ggagcgggcc cgcccaccag tgacccctcc
      541 gtcccggtgg cgccgccgcg cagcagcggg tacgtgacca gcgcggccag cacgacaccc
      601 acgccggcga tcagcgtcag ccacaggagg ctgcgacgcc gcgtgcgcat cggtctacgt
      661 gtccttgagc agcgcacgga ggtccggcgc cgtcatcggt gcctccaggc ggtccacgcc
      721 gtccacggca accaccggca cccggaagcc gtagcgcacc accagctcgt cgtcgcggtc
      781 gatgtccacc cgctggtacc cgtccggacc aatcatctcg tccatcagga cgcgcgcctc
      841 ctcgcaaagg tggcacccgt cgcgctcgta gagcgtgatc tggccctgcc ggctgctcat
      901 ggaccgatga tacggccggc tgcgggcccg gcccgtacgc gccccgcggg tggccggacc
      961 gatgaaacgc gcggtgatgc tcactccgga acctgctcgg tcgcgtcacg cgccatgcgc
     1021 gtgggccagc cgtcgaagat gcgcaatcgt ggtccggccg gccctgtcac ccgctaggtg
     1081 cgtaccttcg agcccggcgg cgcgtggccg tgcgggggcc ggtccggtac gattgatcag
     1141 gcgccgcccc cgtagctcag tggatagagc gccgccgtcc taaggcgggc gtcgggggtt
     1201 cgaatccctc cgggggcgcc atcgaccgag cggaggcgcc gtggccgagc cgcggactcg
     1261 gtttcatcga caggatgttg atgcgctaca ccgggcagcc gtaccgcagg cggctccgag
     1321 cgagaggtct tcgtcatcga gccggccggc tgcggtagtt cgctcgctcc gtcagccctg
     1381 atcgctggcc gctgcccgcc cgagcaggcg tcgcgtcagg tatccgctac tgatgccgtg
     1441 cagcaccacc gaggcggcga tcgccaccga gattgcggcg aacagcggtc tcgcgtccgg
     1501 cgttccgacc tcgcccagcg cgaacccgag gtaatagacc gatccaatcc cgcggatgcc
     1561 gaaccagccg atgaacgccc gctcgttgcg tcgtgtccgg gtggggagta gggccagggc
     1621 caccgccatc ggccgcacga ggaagaggag acctgcggtg acggccagca gcgggaggag
     1681 cagctcgccg atatcggcca gtggaaggac cgtgcggagc atgagcatca cggccatttc
     1741 agcgagcttc tcgacggtga gcgtgaactc gtgcaggcgc tggttgtagt cgtggtgcat
     1801 ctcgtagcga cgaaaggcga cacccgcggc aaagacggcc aggaaaccgt agccctccac
     1861 gatctccacg gcgccataca ccacgaggat cgaggccagc gcgacatagc cgtcgaacgg
     1921 cttcgaaagc caccctcggc ccgcaaccca gtacgagagg gccgcgatgc ccacgccggc
     1981 cgctgcgccc cctgcgattc caacggccac cgcgtagagc acgtcggccg cgagccactc
     2041 ccatagccag gtgctggcca cctggaagct ctcctgtgca ccgaacagcg ccagcatcac
     2101 gaacgggaag gcgaggccgt cgttcagccc cgcctcgctg gtgatggcga agcgggcctc
     2161 gtcctcaggc tcctcgtcgc cgggccccgc cacctggacg tcgtccgcca gcaccgggtc
     2221 tgtcggcgcc agcgccgccc ccagcagcac cgcgcccgga atgctgaggc ccagggcggc
     2281 gtaaccgacc gccgccaccc cggcgatggt cagcggcatg gcaaccagca gcagcatcac
     2341 cgtcgagcgc caggcccgcc aggtgagcgg gcggtccagc ttcaccccgg cgccgaacag
     2401 cgagacgatc accgcgaact cggcaacgtg ggtgaacacc accgggtcca gcgtcgggtc
     2461 gaaccagacg gtttcgctaa cgtccagcac gattcccacg gccaggccga tccccaggta
     2521 gatgatggca gccgagaagg gacgaccctt ctgcaccgag aggaccacgg cgccgatcag
     2581 cagcgcggcg ccaacgacca ccaccagcag ctcgtaggcc tccagctcga tgccgaagcc
     2641 cggcatgcgc gccacgggcg gcaccagccg gtcgaaggga gccatcaccc ggccatccta
     2701 tccgtggctg gcgcgtgttg aaggtcgtga gcggatccgt cgggaacggg caggcatccg
     2761 catgcgatca tgcggcgcaa cgttaacggg cgaggtggca acgatggaca ggcggggcgc
     2821 ggccctggtt gccgaggcgg tcggcacctt cctcttcgtc ttcgtgggag ccggatcggt
     2881 ggtgctggcg acccatgcgg gtgacgcggg gcccggcctg gttggcatcg ccctggccca
     2941 tggcctggcg ctggcggtgc tgatctccgc cttcggcgcc atctccggcg gccacttcaa
     3001 cccggccgtc accgtggccg tacgactggc gggcaggatc gagtggggca acgcggtcgg
     3061 gtacgtgatc gcccagctgc tcggcgcggc cgtcgccgcc ttcgccctgc gggcggtcct
     3121 ccccgagtcg tcgtgggagc cgtcggggat cggcagcccg gcgctcggcc aggggatcgg
     3181 catcggcgcc gggatcgcca tcgaggcggt gctcaccgtg ctgctggtgc tcgcggtgtt
     3241 cggcacggcc atcgacgcgc gggcacctcg cctgggcggg atggccatcg gtctcgcggt
     3301 cacagcagac gttctgatgg gcggtcccct gacgggagcg gccatgaatc cggcgcgctg
     3361 gttcgggccg gcggtcgcat cgggccagct tgataactgg tacgtgtggt gggtgggtcc
     3421 gctgctgggc gggatcgtgg cggcgctgat ctaccgctac gcgctcgagg gtgaggcgga
     3481 gcctgaggtg acgccggcca cccctgaggg ataggccgcc gagttgccgc tggggccagg
     3541 gaaggccccc aaaggacccg cattcacgcc aggtgaccag tagggtcgca aaccggccac
     3601 tggaccgtct gtattcacga gaggtgtgaa ttcacaccct tcccggtgcc ctttccggcg
     3661 ctggtgcgca cctggcgtga atctgcgtgc ctcgccgccc cgccccgccc cgcccccccc
     3721 cacacgtcct cagccggcac cgcccc
//
LOCUS       DATACA010000166         4908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1629138, whole genome shotgun sequence.
ACCESSION   DATACA010000166 DATACA010000000
VERSION     DATACA010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629138"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..425)
                     /locus_tag="VHK28_03400"
     CDS             complement(165..425)
                     /locus_tag="VHK28_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141312.1"
                     /translation="MALIRIAPAPARVGWDRRHTRPVSVQLADRRLTVTAVEAMRDET
                     AAYPAGRGPRVTYLLATDAGHASLVFDHRQRRWYVEALDSAA"
     gene            complement(521..1756)
                     /gene="dinB"
                     /locus_tag="VHK28_03405"
     CDS             complement(521..1756)
                     /gene="dinB"
                     /locus_tag="VHK28_03405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX2141313.1"
                     /translation="MRTILHADLDAFYASVEQLDDPRLRGRPVIVGGDPNARGVVSAA
                     SYEARRFGVHSAMPLRTAQRLCPDGVFLAGRFDRYHELSRMVMAILATFTPLVEPISL
                     DEAFLDVTGSRAAFGDGEEIARRIKERVRAEAGLVVSVGVATNKLCAKVASDLRKPDA
                     LVVVPPGDEAAFLAPLPVSRLWGVGPQARQALSDYGVTTIGQLASLAPGTLRRRFGSH
                     GLDLAQRARGIDPSPVIPEHAPKSVGHEITFDHDVNDLARLETTLLDLAESVASRLRR
                     HDLAAGGVQLKLRYEGFDTITRQAPLDRQTREAEPLYELGVDLLRRARAPDRAVRLIG
                     LTAIHLTDSQQLTLFDAPDRTDRLVRSIDAVRKRFGDEAITRARLVGRVDRRRPDFGM
                     RPDAPPPGGEDEEPPQHGS"
     gene            complement(1766..2920)
                     /locus_tag="VHK28_03410"
     CDS             complement(1766..2920)
                     /locus_tag="VHK28_03410"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2141314.1"
                     /translation="MSAGSALSLRQLNRATLARQGLLTPLPAAEGAARLVHRIGSLQA
                     QHPDWPPVALQARLGPSDQAVDLAAARHRREVVRASLMRVTVHVVAADDFWPMSTVTI
                     GFRARQWRLLYKQDPAASSLGRKITAAHPAALAALRESPLGISEIERVLAAELPGVEI
                     PPHRSLWRHFSGTVPLVQVPYEGETYGRQRYAPAIDWMGQPGEDDADPDRAAAHLAAV
                     YLGAFGPASIDDLVAYVGRGDAIGRWRRALQSLGERIVHFTDPDGRALVDLADAPRPD
                     EDVPAPPRLLARWDSLLLAYATRHRARVLPPEHQATVITRNADVLPTFLVDGVVAGTW
                     LPREDAAGRRIELRPFGALADGDREMLEAQATSLLPTLRGGAFGRYPGTE"
     gene            complement(2941..3615)
                     /gene="lexA"
                     /locus_tag="VHK28_03415"
     CDS             complement(2941..3615)
                     /gene="lexA"
                     /locus_tag="VHK28_03415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX2141315.1"
                     /translation="MTRHDAERKQKIIECIARTVAERGYPPSVREIADAVGLASTSAV
                     HHHLIALEREGLLERGSHSSRALRLTARGEQELETPARRPTHGRVTPFRMPVEREVLA
                     LPVLGEIAAGQPIEAYEDRTETLEVPRSIQAHDGSYVLRVRGKSMIDALIDDGDYVVV
                     QPQATARDGDIVVALLEDNGVTLKRFFRESDRIRLQPANAEMEPIYASEVQIQGKVVG
                     VIRKLA"
     gene            3741..4244
                     /locus_tag="VHK28_03420"
     CDS             3741..4244
                     /locus_tag="VHK28_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX2141316.1"
                     /translation="MNVTGRLRYLRLARLVWRLPTYARLVWGLLRDPRTPVPLKGLLA
                     AGLAYVLVPLDVVPDAVPILGQADDLTVLLLVLDLFIANAPREVREEHMERARSGTAQ
                     LDSDLARLRLLLGDRYDHIRDNLPELLDRYGRLREPGNVKRVLRDWRTRRMRSARAEP
                     DSATLDR"
     gene            4303..>4908
                     /gene="rplI"
                     /locus_tag="VHK28_03425"
     CDS             4303..>4908
                     /gene="rplI"
                     /locus_tag="VHK28_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX2141317.1"
                     /translation="MKVILKHDVKGLGRAGDIKEVKDGYARNFLLPNGAAVVADAGAI
                     KNWERHRSEREERDRSERAAAEATAERLRELKLEIAVKAGEKNRLFGSVTNREIAELL
                     AREGVEVDRHAIHLHEPIKTVGEHRVDVRLMSGVDAQLSVKVIPAPASSATGRSGDCG
                     LTVATPKLSYSGRTLRACAASGPGTGGFPQGAGLSTTRPTYP"
BASE COUNT          723 a         1707 c         1773 g          705 t
ORIGIN      
        1 ggcaccggat ctcgagtcgc cagccgggcg agcagttcgc ggaggggcag gtcggtcagg
       61 ctggtgttgc tggttccctc cactgggcag gccctcgatg cgcggcgaac cagccgcgat
      121 cagggctcgc cacctcgatt gtaggggcgt ggcctgacgc aggactaggc ggcgctgtcc
      181 agcgcctcca cgtaccagcg tcgctggcga tggtcgaaga ccagcgacgc gtggccggca
      241 tcggtcgcca gcaggtaggt aacgcgcgga ccgcggccgg cgggataggc ggcggtctcg
      301 tctcgcatgg cctcgaccgc ggtcaccgtc aggcgccggt ctgccagctg gaccgatacc
      361 gggcgcgtgt ggcggcgatc ccagccgacg cgggcgggag cgggggcgat gcggatgagt
      421 gccatggtct ggtcctagct ccttgcacat cgcctggcag gggcaccgaa caggcgttct
      481 attggtgggt ggccagtcta cgaacatccg ttcggttctg tcaagacccg tgctgtggcg
      541 gctcctcatc ctccccaccc ggaggcgggg cgtcgggccg catcccgaag tccggccgtc
      601 gccggtccac gcgaccgacc agccgagcgc gggtgatggc ttcgtcgccg aatcgcttgc
      661 gcaccgcgtc gatggagcgc accaggcggt ccgtccggtc cggggcgtcg aacagcgtca
      721 gctgctggga atcggtcagg tggatggcgg tcaaaccgat gaggcgtacc gcgcggtcgg
      781 gggcccgtgc tcgtcgcagc aggtcgaccc cgagctcgta gagcggctcg gcctcgcggg
      841 tctgccgatc cagtggcgcc tggcgagtga tggtgtcgaa cccctcgtaa cggagcttga
      901 gctgcactcc gccagccgcc aggtcgtggc gccgcagccg gctggcgacc gactcggcca
      961 ggtccagcag cgtcgtctcc agcctggcaa ggtcgttcac gtcgtggtcg aaggtgatct
     1021 cgtgcccaac cgacttcggc gcgtgctccg ggatcactgg gctgggatcg atccccctgg
     1081 cgcgctgtgc gaggtccaag ccgtggctgc cgaaccgacg ccggagtgtc cccggcgcga
     1141 gggacgccag ctgcccgatg gtggtcaccc cgtagtccga cagtgcctgg cgcgcctgcg
     1201 gacccacccc ccatagccgg ctgacgggga gtggcgccag gaacgcggcc tcgtcgccgg
     1261 gtggcaccac caccagcgca tccggcttgc gcaggtcgga cgccaccttg gcgcacagct
     1321 tgttggtggc caccccgaca ctcaccacca ggccggcctc ggcgcgcact cgctccttga
     1381 tgcgccgcgc gatctcctca ccgtcgccaa aggccgcgcg gctgccggtg acgtccagga
     1441 acgcctcgtc caggctgatg ggctcgacca gtggcgtgaa ggtggccaag atggccatca
     1501 ccatccgcga cagctcgtgg tagcggtcga atcggcccgc caggaagacg ccgtccgggc
     1561 acaggcgttg ggcggtgcgc aagggcatgg ccgagtgcac cccgaagcga cgggcctcgt
     1621 aggacgcggc gctgaccacg ccacgtgcgt tcgggtcgcc gcccacgatt accggccggc
     1681 cgcggagccg cgggtcgtcg agctgctcga cactggcgta gaaggcatcg aggtcggcgt
     1741 gcaggatggt gcgcacggcc cccgatcact ccgtcccggg gtagcgcccg aaggccccac
     1801 cgcgcagcgt cgggagcagg ctggtggcct gcgcctcgag catctcacgg tcgccgtccg
     1861 ccagcgcgcc gaagggtcgc agctcgatcc ggcggccggc cgcatcctcg cgcggcagcc
     1921 aggtgccagc caccacgccg tcgaccagga aggtgggcag cacgtccgca ttgcgggtga
     1981 tgacggtggc ctggtgctcc ggcggcagga cccgggcccg atggcgcgtg gcgtaggcga
     2041 gcagcaggct gtcccagcgg gccagcagcc gcggcggggc cgggacgtcc tcgtccggcc
     2101 gcggggcatc ggccaggtcg acgagggcgc ggccgtccgg atcggtgaag tggacgatcc
     2161 gttctccgag gctctgcagt gcgcggcgcc aacgcccgat ggcatccccc cggccgacgt
     2221 aggcgaccag gtcatcaatg ctggccggcc cgaaggcgcc aaggtacacg gcggccaggt
     2281 gggcggcggc ccggtcggga tcggcgtcgt cttcgcccgg ctggcccatc cagtcgatgg
     2341 ccggcgcata ccgctgccgg ccataggtct caccctcgta cgggacctgc accaggggca
     2401 cggtaccgct gaaatgccgc cacagggacc tgtggggtgg aatctccacc cccgggagct
     2461 cggcggccag gacgcgctcg atctcgctga tgccaagcgg cgactcgcgg agtgcggcca
     2521 gcgcagctgg atgggcggcc gtgatcttgc ggcccaggga tgacgcagcc ggatcctgct
     2581 tgtacagcag gcgccactgg cgcgcgcgaa agccgatggt gacggtggac atcggccaga
     2641 agtcgtcggc cgccaccacg tggaccgtga cccgcatcag cgaggcgcgc accacctcgc
     2701 ggcgatggcg agcggccgcg aggtcgaccg cttggtccga tggccccagc cgcgcctgca
     2761 gcgcgaccgg cggccagtcc gggtgctgag cctgcagcga gccgatgcgg tgtaccagcc
     2821 gcgcggcccc ctcagcggca ggcagcggag tgagcaatcc ctggcgcgcc agcgtggcgc
     2881 ggttcagctg gcgcaggctg agcgccgagc cggccgacac gagtccagcc agccctggtg
     2941 tcaggcgagc ttgcggatga ccccgacgac cttgccctgg atctgcactt ccgacgcgta
     3001 gatgggctcc atctccgcgt tcgccggctg caggcggatg cggtcgctct ctcggaagaa
     3061 ccgcttcagg gtcacgccgt tgtcctccag cagggcgacc acgatgtcgc cgtcccgggc
     3121 tgtggcctgc ggctggacga ccacgtagtc accgtcgtcg atgagggcgt cgatcattga
     3181 cttgccgcgc acgcgcagga cgtacgagcc gtcatgggcc tggatcgagc gggggacctc
     3241 gagcgtctcg gtcctgtcct cgtacgcctc gatgggctgt ccggcggcga tctcgccgag
     3301 cacgggaagg gccagaacct cgcgctcgac cggcatgcgg aagggtgtca cgcggccgtg
     3361 ggtgggtcgc cgcgccggcg tttcgagctc ctgctcaccg cgggcggtga ggcgcagggc
     3421 gcgggatgag tgcgagcccc gctcgagcag tccctcgcgc tccagcgcaa tgagatggtg
     3481 gtgcacggcc gaggtcgacg ccaggccgac ggcgtccgcg atctcgcgca cggacggcgg
     3541 gtagccgcgc tccgcaacgg ttcgcgcgat gcactcaatg atcttctgct tgcgctcggc
     3601 atcgtgcctg gtcacgggca gctcctgctt cgacggtcgc cgagcatgct agccgaacag
     3661 acgttctatt gtcaaccgag accggccggt cgcgctatga tcggggacca tttgagcctc
     3721 tgctcggagc gagaccccgc gtgaatgtga ccggtcggct gcgctacctg cgcctggcgc
     3781 gcctcgtgtg gcgcctgccg acctatgccc ggctggtgtg gggcctgctg cgcgacccgc
     3841 gcacgccggt ccctctcaag ggcctgctgg ccgccgggtt ggcctacgta ctggtaccgc
     3901 tcgacgtggt gcccgatgcc gtgcccatcc tcggccaggc ggatgacctc accgtcctgc
     3961 tgctggtgct ggacctgttc atcgccaacg caccacgcga ggtgcgcgag gagcacatgg
     4021 agcgcgcgag gagcggcacc gcacagctgg acagcgacct ggcgcgcctc cgcctgctgc
     4081 tgggagatcg ctacgaccat atccgcgaca acctccccga gctcctggac cgatacggac
     4141 ggctgcgcga gcccggcaat gtgaagcggg tgctgcgaga ctggcgaacg cgacggatgc
     4201 gctctgcccg ggccgaaccc gactccgcca ccctggaccg atgagaaccg tgccctgcgc
     4261 cccggcaggc cggcggttgc cacgaaagga accccgaact cgatgaaggt catcctcaag
     4321 cacgacgtga agggcctcgg acgggccggc gatatcaagg aggtcaagga tgggtacgcc
     4381 cgtaacttcc tgctgccgaa cggcgccgcc gtggtggcgg acgccggcgc gatcaagaac
     4441 tgggagcggc accggtcaga gcgcgaggag cgcgaccgct cggagcgcgc cgccgccgag
     4501 gccacggccg agcgcctgcg cgagctgaag ctcgagatcg ccgtcaaggc tggcgagaag
     4561 aaccgtctgt tcggctcggt caccaaccgc gagatcgccg agctgctggc gcgcgagggg
     4621 gtcgaggtcg accgccacgc gatccacctg cacgagccga tcaagaccgt cggagagcat
     4681 cgcgtcgacg tgcggctgat gtcgggcgtg gatgcccagc tgtccgtgaa ggtcatcccc
     4741 gccccggcga gctcggcaac cgggcgcagc ggcgactgtg gcctgacggt cgcgacgccc
     4801 aagctgtcat attccggccg caccctgcgc gcatgcgcag catccggccc gggcaccggc
     4861 ggttttccac aaggggcggg attgtccaca acccgcccca cttatcca
//
LOCUS       DATACA010000167         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_344497, whole genome shotgun sequence.
ACCESSION   DATACA010000167 DATACA010000000
VERSION     DATACA010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344497"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VHK28_03430"
     CDS             complement(<1..721)
                     /locus_tag="VHK28_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX2141318.1"
                     /translation="MTTDEQLLQRAAELAAEYRRSLPERHVGPRADAATIGERLGSLP
                     ESGVGPMQVLEELVEAVDPGLVASAGPRYFGFVIGSVLPAAAAADWMTTTWAQNAALP
                     ASSPAAAAAEQVAGEWMLELLGLPASASIGLPTGAGLGNTVGLAAGRHAVLARAGWDV
                     EAQGLYGAPEIRVVVGEEAHATLLTGLQYLGLGRDRVIRVPTDDQGRIRADAFRAAMS
                     EVGDGPLIVAAQAGNVNTGGFD"
     gene            complement(747..1532)
                     /locus_tag="VHK28_03435"
     CDS             complement(747..1532)
                     /locus_tag="VHK28_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2141319.1"
                     /translation="MGGVLVPPALDTSELETRVREMYRAVAEAPEGRYHFELGRALAD
                     RLGYDPRALDSVPADAVDSFAGVGHFFDLVELRPGESVLDLGSGSGMDAFLAAAQVGS
                     EGRVVGVDMTPEQLAKAERLREAAGLENAKFVEGQIERLSADEASFDAVISNGVINLS
                     PQKDRVFAEAFRVLRPGGRLAIADIIAERALPDDVVCNADLWASCIGGAAESGAYRQA
                     IADAGLILERTRVNPYAFISGQARRASVTYGVQSVSLLARKPA"
     gene            complement(1534..2100)
                     /locus_tag="VHK28_03440"
     CDS             complement(1534..2100)
                     /locus_tag="VHK28_03440"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2141320.1"
                     /translation="MTTETSTSNGIDVEQLVQTIDAIQADPSVGEFTFRAASTWRGGT
                     HNVGRIGSFRHAGADDQSRIAPFVLEGDEPPVLLGQNHGPNAVELVLQALAFCYAVGY
                     VANAAARGIQISSMEYEIEGDLDVRPFLGLEGRRPGFTSVRAKARVSSPNATQAQLEE
                     LCQYVQDTSPVRDIIANATPVSTSLEVA"
     gene            2254..4821
                     /locus_tag="VHK28_03445"
     CDS             2254..4821
                     /locus_tag="VHK28_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141321.1"
                     /translation="MQLLERDRPLSLLSEAHAAASDGRGSVVLLAGEAGIGKTSLVNR
                     FAAELGPQARLLLGMCDDLRIPRPLGPLRNLAGAPSAELVEAIATARPATEVGAALLA
                     ELERAPQPGVLVLEDVHWADEATLDAVTVVARRIEGVPALLLLTFRPGEIGDDHPLRS
                     LLGAVPAAAARHIALAPLSREGVASLAGEQADEVYVATGGNPFFVVQLLAAGPGELPP
                     SVANAVLAQAARLDPDARRLVELVSVVPSRIEATLVDAIMPGWAVAAEEPERRGLLEV
                     GPTDVRFRHELARLAIRSSVPVARRRRLHAEVLAALRAAGADAADIVHHAEAAGDLDA
                     VAEHALVAGRQAAAVGANREAYAHFRRAADFADRLDSSGRATLFEELAGSAYVANQVT
                     DALAAIERAISIHVERDDRVAIGRCTRLKSRIHWYAGDGAAALDAARSAVAILEPIGP
                     SGELARAYSALSQLAMLAGRVEETVSWGERAVNLATTFGDDRGRAHALINIGLARAGV
                     DPDDTAVLMEGYRAADAAGDRHEATRALLGLSGILFEWVRPAEAIRIGDEAIAYAERY
                     QVDTLLAYLKAMRAWMHLRAGEWAEAERLAWAEIERDVTVAQLVAKTVLAALAVRRGD
                     ADAAERLAEVVLQADRTGELQHIVPVLEIEVARSMTTGEPMPVAHLERALTLVDAKDR
                     ELGWDAGRLVAWARSAGLKISVQARMPAPHAAIAAGDWRGAADAFGAVGWTYDRALFL
                     LLLEDEAALAEALEVARDLDARPLADRVTTLMRDLGYRIPRGVRESTRANPMNLTDRQ
                     LEVLRLLAEGLTNAEIAERLVVSPRTAEHHVAAVLGKLDAASRREAVRSATALGMLG"
     gene            complement(4832..>5842)
                     /locus_tag="VHK28_03450"
     CDS             complement(4832..>5842)
                     /locus_tag="VHK28_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2141322.1"
                     /translation="RIGNQVDRHFFTTLLTAVLGFVVAATIVVTLLERQLTLADFGNT
                     FYWAITTVIGSGDASYVTSPGGFIVGWLLAFFGVALVAAMTGAVVGFVIDFLLKEGQG
                     MGAAGFRDHIVVCGWNTTARDLIEELRTDEYKTRVVVLHDTERNPAGDGIYFVRGDIT
                     AEADLRRTGIDEASSAIVFPADGSNEADMRSILCVMAIESLAPDVRTVVEANNPAHVE
                     HFRRAKADEVLVTSRLASRLLARSALYPGLSEMVTDMVSGGEGSELYRIAIPEDMCGL
                     SVDEFSARLRAEHRATLLAVSRRGRSSINPPSDFKMEPGDDAVVVAESLGTLAPLRLQ
                     HD"
BASE COUNT          791 a         2115 c         2073 g          863 t
ORIGIN      
        1 gatcgaagcc gccggtgttc acgttcccgg cctgcgcggc cacgatcagc gggccgtcgc
       61 cgacctcgga catcgcggcg cggaaggcgt cggcccgaat gcgtccctgg tcatcggtcg
      121 gcacgcggat cacgcggtcc cggcccaggc ccaggtactg cagccccgtg agcagggtgg
      181 cgtgcgcctc ctcgcccacc accacccgga tctccggagc gccgtacagc ccctgggcct
      241 ccacgtccca gccggcccgg gccagcaccg catgacggcc ggcggccagg ccaaccgtgt
      301 ttcccagccc cgcaccggtc ggaaggccga tgctggcgct ggccggcaag cccagcagct
      361 ccagcatcca ctccccggcg acctgctccg cggcggccgc ggccggcgac gaggcgggca
      421 gcgccgcgtt ctgcgcccag gtggtcgtca tccagtccgc ggcggcggcg gccggcagca
      481 cactcccgat cacgaatccg aagtagcgcg gcccagccga cgccaccagg ccggggtcga
      541 ccgcctccac cagctcctcg agcacctgca tgggtcccac gccgctttcg ggcagtgatc
      601 ccagcctctc cccgatggtg gccgcgtcgg cccgcgggcc gacgtggcgt tcgggcaggg
      661 agcgccggta ctcggccgcc agctccgcgg cgcgttgcag cagctgctcg tcagtcgtca
      721 ccttgcacca ctcctccggt cgaccttcag gcgggcttgc gggccagcag cgacacgctc
      781 tggacgccgt acgtcacgct cgcgcgccgc gcttgccccg agatgaacgc gtatgggttc
      841 acgcgtgtcc gctcgaggat aaggcctgcg tcggcgatcg cctgccggta ggcgccggat
      901 tcggctgccc cgccgatgca ggaagcccac aggtccgcat tgcagactac gtcgtcgggc
      961 agtgcgcgct cggcgatgat gtcggcgatc gccaggcgcc cgccgggccg aaggacgcgg
     1021 aacgcctcgg cgaacacgcg gtccttctgc ggcgagaggt tgatgacgcc gttggagatg
     1081 accgcgtcga agctcgcctc atccgctgac agccgctcga tctggccctc gacgaacttc
     1141 gcgttctcca gcccggccgc ctcccgcagc cgttcggcct tggccagctg ctccggcgtc
     1201 atgtccaccc cgaccacgcg gccttccgac ccgacctggg cggccgccag gaaggcgtcc
     1261 atccccgagc ccgagccgag gtcgagcacc gactcgcccg gacgaagctc caccaggtcg
     1321 aagaagtgcc ccaccccggc gaacgagtcc accgcgtcgg ccgggaccga atccagcgcc
     1381 cgcgggtcgt agccgagccg gtcggccagc gcccgtccca gctcgaagtg gtagcggccc
     1441 tcgggggcct ctgccaccgc gcggtacatc tctctgaccc tggtctccag ctcgctcgtg
     1501 tccagcgctg gggggacgag gacgccgccc atctcaggct acctccaggc tggtggacac
     1561 cggagtggcg ttggcgatga tgtcgcgcac cggcgaggtg tcctggacgt actggcacag
     1621 ctcctcgagc tgcgcctggg tggcgttcgg gctggacacc cgtgccttgg cccgcaccga
     1681 ggtgaagccg gggcgccgtc cctccagccc caggaaggga cgcacgtcga ggtcaccctc
     1741 gatctcgtac tccatgctgc tgatctggat gccgcgagcg gcggcgttcg ccacgtagcc
     1801 gacggcgtag cagaaggcca gcgcctgcag gaccagctcc actgcgttcg gcccgtggtt
     1861 ctgcccaagc aggacgggtg gctcgtcgcc ctccaggacg aacggtgcga tgcggctctg
     1921 gtcgtccgcg ccggcatgcc tgaaggaacc gatgcgcccc acgttgtgcg tcccaccccg
     1981 ccaggtgctg gccgcccgga aggtgaactc acccacgctc ggatcggctt ggatggcgtc
     2041 gatggtctgc accagctgct cgacgtcgat gccgttgctg gtgctggtct cggtggtcat
     2101 tttgctcgcc tcctttcgtt ctggcgtccc tgatgacgct gcgggtcacg ctaggcgggc
     2161 ggtgagcgca tggcatcggc aggaaccacc catttcgggc gtccgggcta cccacctgtt
     2221 tccgcttgtg cacctcgcta cgctggaggg accgtgcagc tgctggagcg cgatcgcccg
     2281 ctctccctcc tgtccgaggc gcatgccgcg gcgtcggatg gccgcggaag cgtcgtcctg
     2341 ctggccgggg aggcgggcat cggcaagacg tccctggtca accgcttcgc ggccgagctg
     2401 ggcccgcagg cccgcctgct gctcgggatg tgcgacgacc tccgcatccc tcgaccgctc
     2461 gggccgctgc ggaacctggc cggcgctccc tcggcggagc tggtggaggc gatcgccacc
     2521 gctcgcccgg ccaccgaggt cggtgccgcc ttgctcgccg agctggagcg cgccccgcag
     2581 ccgggggtgc tggtcctgga ggacgtgcac tgggcggacg aggcgacgct cgacgcggtc
     2641 accgtcgtgg cccgccggat cgagggcgtc cccgcgctgc tcctgctcac cttccggccg
     2701 ggcgagatcg gggacgatca cccgcttcgc agcctgctcg gtgcggtgcc cgcggccgcc
     2761 gcccgtcaca tcgccctggc gccgctctcc cgagaggggg tggcctcgct ggccggtgag
     2821 caggcggacg aggtctacgt ggccaccggc ggcaacccgt tcttcgtggt ccagctgctg
     2881 gcggccggcc cgggggagct gccgccctcg gtggccaatg cggtcctcgc ccaggccgcg
     2941 cggctggatc ccgatgcgcg gcggctggtc gagctcgtgt cggtcgtccc ctcgcgcatc
     3001 gaggcaacgc tggtcgacgc catcatgccc ggctgggcgg tggccgccga ggaacccgag
     3061 cggcgagggc tcctggaggt gggtccgacg gacgttcgct tccggcacga gctggcgcgc
     3121 ctggccatcc gttcgagcgt ccccgtggcc cgccgacgcc ggctccacgc cgaggtcctc
     3181 gctgcgctgc gcgccgcggg agcggatgcc gcagacatcg tccaccacgc ggaggccgcc
     3241 ggcgatctgg acgccgttgc cgagcacgcg ctggtagccg gccgccaggc agcggcggtg
     3301 ggcgccaatc gcgaggcgta cgcccatttt cgtcgggcgg cggacttcgc cgaccgcctc
     3361 gactcgtccg gtcgcgccac cctgttcgag gagctggccg ggtcggccta cgtcgccaac
     3421 caggtgaccg atgcgctggc ggccatcgaa agggcgatct cgatacacgt cgagcgcgac
     3481 gaccgggtgg ccatcggtag atgcacccgc ctgaagtcgc gcatccactg gtacgccggc
     3541 gacggtgcgg ccgccctcga cgccgctcgc agcgcggtcg ccatcctgga acccatcggt
     3601 ccatccggtg agctcgcccg ggcgtacagc gcgctctcgc agctggccat gctggcgggg
     3661 cgagtggagg agaccgtcag ctggggtgag cgggccgtca acctggccac caccttcggc
     3721 gacgaccgcg gacgagccca tgccctgatc aacattggcc tggctcgggc gggggtcgac
     3781 ccggacgaca cggcggtcct gatggagggc taccgcgcgg cggacgcggc gggggaccgg
     3841 cacgaggcga cgcgggccct gctgggcttg agcggcatcc tcttcgaatg ggtgaggccg
     3901 gccgaggcga tccgcatcgg cgacgaggcc atcgcctacg ccgagcgcta ccaggttgac
     3961 acgctgctcg cctatctgaa ggcgatgcgc gcctggatgc acctgcgtgc cggtgagtgg
     4021 gcggaggcgg agcgactggc gtgggcggag atcgagcggg acgtcaccgt ggcgcagctg
     4081 gtcgccaaga cggtgctggc cgctctcgcc gtgcgtcgcg gcgatgcgga tgccgcggag
     4141 cggctcgccg aggtcgtgct gcaggcggat cggaccgggg agctgcagca catcgtgccg
     4201 gtgctggaga tcgaggtggc gcggtcgatg accactggtg aaccgatgcc ggtcgcccac
     4261 ctggagcgcg cgctgaccct cgtcgacgcg aaggaccgcg agctggggtg ggatgctgga
     4321 cgcctggttg cctgggcgcg ctcggctggc ctgaagatca gcgtgcaggc gcggatgccc
     4381 gctccgcatg ccgccatcgc cgccggcgac tggcgtgggg cggccgacgc cttcggcgcg
     4441 gtgggctgga cctatgaccg cgccctgttc ctcctgctgc tggaggacga ggcggcactg
     4501 gccgaggcgc tcgaggtggc ccgcgacctg gacgcgcgcc cgctggccga tcgggtcacc
     4561 acgctcatgc gtgatctcgg gtatcgcatc cctcgtggcg tccgcgagtc gacgcgggca
     4621 aaccccatga accttaccga ccggcagctc gaggtgctgc gactgctggc cgagggactg
     4681 accaacgcgg agatcgccga gcgcctggtc gtgtcacctc gcaccgccga gcatcacgtg
     4741 gcggctgtgc tcggcaagct ggacgccgcc tcccgccgag aggcggttcg cagcgcgacc
     4801 gcgctcggca tgctgggttg agcgtccggg ttcagtcgtg ctggaggcgc agcggcgcca
     4861 gcgtgcccag cgactcggcc accaccaccg catcgtcgcc gggttccatc ttgaagtcgg
     4921 acggtgggtt gatggacgac cgcccgcggc ggctgacggc cagcagggtg gcccgatgct
     4981 ccgcccgcag gcgcgccgaa aactcgtcca cggacagacc gcacatgtcc tcggggatgg
     5041 cgatgcggta cagctccgac ccttcgccgc cggagaccat gtcggtcacc atctccgaca
     5101 gcccggggta gagcgccgac cgcgccagca gccgtgacgc gaggcgtgag gtgaccagca
     5161 cctcatcggc cttggcccgc cggaagtgct cgacgtgggc cgggttgttg gcctccacca
     5221 cggtccgcac gtccggcgcg agcgactcga tggccatgac gcacaggatc gagcgcatat
     5281 cggcctcgtt gctgccgtcg gctgggaaga cgatggcgct gctcgcctcg tcgatgcccg
     5341 tgcgtcgcag gtcggcctcg gcggtgatgt cgcctcgcac gaagtagatc ccgtcgcccg
     5401 ccgggttgcg ctccgtatcg tggagcacga ccacgcgcgt cttgtattcg tccgtccgca
     5461 gctcttcgat caggtcgcgg gcggtcgtgt tccagccgca gacgacgatg tggtcgcgga
     5521 aaccggccgc acccattccc tggccctcct tgagcagaaa gtcaatgacg aaccccacca
     5581 ccgccccggt catggccgct accagcgcca cgccgaagaa ggcgagcagc cagcccacga
     5641 tgaagccgcc gggtgacgtc acgtagctgg cgtcgcctga gccgatgacc gtggtgatcg
     5701 cccagtagaa cgtgttgccg aagtcggcca gcgtgagctg gcgctcgagc aaggtcacca
     5761 cgatggtcgc cgccacgacg aagcccagca ccgccgtcag cagggtcgtg aagaaatgcc
     5821 ggtcgacctg gttcccgatg cg
//
LOCUS       DATACA010000168         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2513305, whole genome shotgun sequence.
ACCESSION   DATACA010000168 DATACA010000000
VERSION     DATACA010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513305"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VHK28_03455"
     CDS             <1..385
                     /locus_tag="VHK28_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141323.1"
                     /translation="LAAAAVAPTRELAIAAWIPFGIGMGAFLSADWALMADVIPKATA
                     GRYMGILNAGTAMAGPLYIVIAGPAQDLSGALFGEAVGPRVAIGIAALFVVGAALALT
                     RVNPRRREDPVPEAEPAAARAVGAA"
     gene            382..1140
                     /locus_tag="VHK28_03460"
     CDS             382..1140
                     /locus_tag="VHK28_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX2141324.1"
                     /translation="MSAASSPADRARRTGPRELSVRPDRRYRVLWGANRTLVRLLFDV
                     RASGLERWPSAPFCLVANHHNGWDPLLVMAVAPFDPRISWFGPREADFSHGFKNRVMA
                     FFGGVIPYHPQMTTLTSAVRAVRRVFAAGGVLGIFAEGRVGFRESELLPFEDGAVVFA
                     STAEVPIVPCAIVGSSWLWFRRRVDVRFGGPIPTRGVRSREARAELEAVVRAAMLELM
                     PDTEPAVPRRRPLRWLGDLLTGADDLARREGSDG"
     gene            1153..1425
                     /locus_tag="VHK28_03465"
     CDS             1153..1425
                     /locus_tag="VHK28_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141325.1"
                     /translation="MLPVIWAVFAIAAVGGFLVLAAYWLDVQDRPDLTFRARVAWSVA
                     ILAFPLTIPAYAFAGGGGWPPFLRAVSFLPAFALLLFFGFAFGLFT"
     gene            complement(1442..1963)
                     /locus_tag="VHK28_03470"
     CDS             complement(1442..1963)
                     /locus_tag="VHK28_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141326.1"
                     /translation="MLVLALTFVPAARVLACSCMDLPFDEHVALADLAVVGVVGGADE
                     VRIDEGNGLVSMGTTYTLVVEEVLKGEVRVGEAALFHSEVGTCGMAFSIGQRWRMTAS
                     RQADTYVTSICSGNELLAENVPVPAVAPAVEEDGGGPGEIPAGVLLALGGFAMVAVVS
                     VVAFGRAERKTSS"
     gene            complement(2080..2676)
                     /locus_tag="VHK28_03475"
     CDS             complement(2080..2676)
                     /locus_tag="VHK28_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141327.1"
                     /translation="MTDGHRRVTERGAAPPRPADMRWRLAGRLSRVVAALLLGSIASF
                     GPVAEVLACDCSAIAPARALAEADAAFVGVVTGTLKQIDEPAAVGANYLFLVEEVLKG
                     QGGAEVQVSTNATDCAASFRAGERWRVYAYVDAGVLTTHLCAGNQRLAEGAAPVSQPA
                     PETAGGLPSEALFGGGAFLVVVLVAAAGFLLGTRPAKR"
     gene            2696..4117
                     /locus_tag="VHK28_03480"
     CDS             2696..4117
                     /locus_tag="VHK28_03480"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX2141328.1"
                     /translation="MTTTVRGVDAALSYLREHADEHLKGLDEFLRLESVSADPARRDQ
                     VRRTAQWIVDQLTRMGVDDAQLHETSSHPIVTGSWLHAGDDRPTVLVYCHYDVQPADP
                     LEEWVRPPFEPRYENDIVYARGSGDDKGQLYMHLKAAEAWLQGAGGLPVNLRFFFEGD
                     EEVGSLPVEEFIASHPELLEADICVVSDTDMQDDEGTPAITYGLRGIAYWEIKVSGPF
                     QDVHSGTYGGGVDNPANVLVNLLSGLTDEHGRVTVPGFYDKVRPMTDEERAAYAALPF
                     DETEWAKGLGVPGPMDGDPDHTLPERLSARPTFDIHGLWGGYAGDGPKTIIPAWAAAK
                     FSTRLVPDQDHREIERMMVEHIERMAPPTVKVEVRIVHGGQPAITPIDHPAVRIAARA
                     LEATFGKPPQMHRSGGSIPVVAALESGIGVKSVLVGFASPNGNFHAPNEWLPLANYRG
                     GMEAIVRLWQELGDAAPAELRGG"
     gene            4129..4782
                     /locus_tag="VHK28_03485"
     CDS             4129..4782
                     /locus_tag="VHK28_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141329.1"
                     /translation="MSGEHDRRTNPDDPWLPLGAASRLLGVGPDTLRRWADSGQVESY
                     TTPGGHRRFLRSSLEAMVNAPRRSRYGVARLAGSAGTISGDVHRRVARVGEAAQPWQA
                     RLNKEQRDDFRRWGQRTFQLVIDYVAAGRRAERELLLSEAEKMGALYGAEASRAGLSL
                     AEAVEAFLFYRSPVLEAIGSHLRRRAADVSDVTVAYREATAAIDEVLNALVSSYRGS"
BASE COUNT          735 a         1889 c         1782 g          762 t
ORIGIN      
        1 cctggcggcc gccgccgtgg cccccacgcg tgagctggcc atcgcggcct ggataccgtt
       61 cgggatcggg atgggcgcgt tcctctccgc ggactgggcg ctcatggccg atgtcatccc
      121 caaggccacc gccggtcgct acatgggcat cctcaacgct gggaccgcca tggccggccc
      181 gctgtacatc gtgatcgccg gaccggcgca ggacctgtcg ggcgccctct tcggggaggc
      241 agtcggtccg cgtgtggcga tcggaatcgc ggcgctattc gtggtggggg ccgcgctggc
      301 tctcacgcgc gtcaatcccc ggcgtcggga agacccggtg ccagaggcgg agccggcggc
      361 cgcccgggcg gtcggcgcgg cgtgagcgca gcctccagcc cggcggaccg cgcccggcgg
      421 accgggccgc gcgagctctc cgttcgcccc gaccggcgct atcgggtgct gtggggggcg
      481 aaccggacct tggtgcgcct cctcttcgac gtgcgcgcca gcggcctcga gcggtggcct
      541 tcggcgccgt tctgcctggt cgccaaccac cacaacggct gggacccact gctggtcatg
      601 gcggtcgctc ccttcgatcc acggatcagt tggttcggac cgcgcgaggc cgatttctca
      661 cacgggttca agaaccgggt gatggccttt ttcggcggcg tgatccccta ccacccgcag
      721 atgaccacgc tgacgtccgc agtgcgcgcg gtgcgccgcg tgttcgcggc cggcggcgtg
      781 ctcggcatct tcgccgaggg tcgggtcggc ttccgcgagt cggagctgct ccccttcgag
      841 gacggcgccg tcgtcttcgc ctccaccgcc gaggtgccga tcgtgccgtg cgccatcgtc
      901 ggcagcagct ggctgtggtt caggcggcgc gtggacgtgc gcttcggagg accgattccc
      961 actcgcggcg tccgcagccg cgaagcgcgg gcggagctgg aagccgtcgt gcgtgctgcc
     1021 atgctcgagc tgatgccgga tacggagccg gcggtcccgc gccggcggcc actgcgatgg
     1081 ctgggggatc tgctgaccgg ggcggacgac ctggcgcggc gggagggctc ggacgggtag
     1141 catcggtccc gcatgctccc ggtcatctgg gcggtctttg ccatcgccgc ggtcggcggg
     1201 ttcctggtgc tcgcggccta ctggctcgac gtgcaggacc ggcccgacct cacgttccgc
     1261 gcccgcgtgg cgtggtcggt cgccatcctg gccttcccgc tgaccatccc cgcctacgcc
     1321 ttcgccggcg gtggcggctg gccaccattc ctgcgcgccg tgtcgttcct gcccgccttc
     1381 gcgctgctcc tgttcttcgg gttcgcattc gggctgttca cctaggcccc gggtggcgcc
     1441 gtcaggagga ggtcttccgc tccgctcgcc cgaatgccac aaccgatacg acggccacca
     1501 tcgcgaatcc gccgagcgcc agcagcacgc ccgccgggat ctcccctggc ccccctccgt
     1561 cctcttcgac ggctggcgcg acggcgggca ccgggacgtt ctcggcgagc agctcgttgc
     1621 cggaacagat gctggtgaca taggtatccg cctggcgaga tgcggtcatt cgccaccgct
     1681 gaccgatgct gaacgccatc ccgcaggtcc caacctcgct gtggaagagc gctgcctcgc
     1741 ccacccgaac ttcgcccttg aggacctcct ccacgacaag ggtgtaggtc gtccccatcg
     1801 agaccagccc attgccctcg tcgatcctca cctcgtcggc gcctcccacc acgcccacca
     1861 cggcgaggtc agcgagcgcg acgtgctcat cgaagggaag gtccatgcag gagcaggcga
     1921 ggacgcgtgc ggccggcacg aaggtgagcg ccagtaccag catcgccgga gcaaacagga
     1981 cggtcgccgt tcgacgggtc acaccccttc gacgcaaccg tccgcccgtt cgttgcgggc
     2041 cgcgccacgc acgaacgcgc tcacgagagc gcgaaccttt catcgtttcg ccggcctggt
     2101 gccgagcaga aacccggccg cggccaccag caccaccacc agaaacgctc cgcctccaaa
     2161 aagggcttcg gacggcagtc caccggccgt ctcgggagcc ggctgtgaga cgggggcggc
     2221 accctccgcc aaccgctggt tcccggcaca taggtgcgtg gtcagcacac ccgcgtcgac
     2281 gtaggcgtag acgcgccacc gctcgccggc ccggaagctg gctgcacagt ccgtcgcgtt
     2341 ggtggagacc tgcacctcag cgccgccctg gcccttcagc acctcctcca ccaggaacag
     2401 gtagttggca ccgacagccg ccggctcgtc gatctgcttc agggttccgg tgacgacccc
     2461 gacgaacgcc gcgtccgcct ccgccagggc gcgagccggg gcgatggcgg agcaatcgca
     2521 ggccaggacc tcggccacgg gcccgaacga cgcgatcgaa ccgaggagca gtgccgctac
     2581 aaccctggat agtcgccccg ccagtcgcca gcgcatgtcc gcaggacgcg gcggcgcggc
     2641 cccgcgttcc gtcactcgcc ggtgaccatc ggtcatcccg cctatcatcg cgaccatgac
     2701 aaccaccgtg cgcggtgtcg acgccgccct cagctacctg cgcgagcacg ccgatgagca
     2761 cctcaagggg ctggacgagt tcctgcggct ggaatcggtc agcgccgacc cggcccgccg
     2821 tgaccaggta cgccgcaccg cgcagtggat cgtggaccag ctgacccgca tgggggtaga
     2881 tgacgcgcag ctgcacgaga ccagcagcca cccgatcgtg accggaagct ggctgcacgc
     2941 cggcgacgac aggccgaccg tgctcgtcta ctgccactac gacgtgcagc ctgccgatcc
     3001 gctcgaggag tgggtgcgcc cgcccttcga gccgcgatac gagaacgaca tcgtctacgc
     3061 ccgcggctcc ggcgacgaca agggccagct gtacatgcac ctcaaggcgg ccgaggcctg
     3121 gctgcagggc gcaggcgggc tcccggtcaa cctgcgcttc ttcttcgagg gcgacgagga
     3181 ggtcggtagc ctgccggtgg aggagttcat cgcctcgcac cccgagctgc tggaggcgga
     3241 catctgcgtg gtgtcggaca ccgacatgca ggacgacgag ggcacgccgg ccatcaccta
     3301 cgggctgcgc ggcatcgcct actgggagat caaggtctcc gggccgttcc aggacgtgca
     3361 ctccggcacg tacggcggcg gcgtcgacaa cccggccaac gtgctggtca acctcctctc
     3421 cggcctgacc gatgagcacg gccgggtcac ggttcccggc ttctacgaca aggtcagacc
     3481 aatgaccgat gaggagcggg cagcctacgc cgcgcttccc ttcgacgaga ccgagtgggc
     3541 caaggggctg ggcgttcccg gaccgatgga cggcgacccg gatcacaccc tccccgagcg
     3601 gctctcggcg cggccgactt tcgacatcca tgggctgtgg ggtggatacg ccggcgatgg
     3661 gcccaagacc atcatccccg cctgggcggc cgccaagttc agcacccggt tggtgccgga
     3721 ccaggaccac cgcgagatcg agcggatgat ggtcgagcac atcgagcgca tggccccgcc
     3781 gacggtcaag gtcgaggtgc gcatcgtcca cggcgggcag ccggccatca cgcccatcga
     3841 ccaccccgcc gtacgcatcg ccgcccgcgc gctggaggcg accttcggga agccgccgca
     3901 gatgcatcgg tccggtgggt ccatcccggt ggtcgctgcg ctcgagtcgg ggatcggcgt
     3961 caagagcgtg ctggtcggct tcgcgtcgcc gaacggcaac ttccacgccc caaacgagtg
     4021 gctcccgctg gccaactacc gtggcgggat ggaggccatc gtccggctct ggcaggagct
     4081 gggggatgcc gcgccggcgg agctgcgcgg gggctgaccg gcagcacggt gagcggcgag
     4141 cacgaccggc gcacgaatcc cgacgacccc tggctgcccc tgggtgccgc ctcgcgcctg
     4201 ctgggcgtgg ggccggacac cctgcgccgc tgggcggact ccggccaggt ggagagctac
     4261 accacgccag gcgggcatcg ccgcttcctg cggtcgagcc tcgaggcaat ggtcaacgcc
     4321 ccgcgccgct cgcgctacgg cgtggcgcgg ctggccggtt cggcgggcac catctcgggc
     4381 gacgtccacc gccgcgtggc acgcgtcggc gaggcggccc agccctggca ggcgcgtttg
     4441 aacaaggagc agcgcgatga cttccgccgc tggggccagc gcaccttcca gctggtgatc
     4501 gactacgtgg ccgccggtcg ccgcgccgag cgggagctgc tgctcagcga ggccgagaag
     4561 atgggcgccc tgtacggcgc ggaggcctcc cgcgccggcc tctcgctggc cgaggcggtc
     4621 gaggccttcc tgttctaccg ctcgccggtg ctggaggcga tcggatccca cttgcgacgc
     4681 cgcgcggccg atgtctccga cgtaacggtc gcctatcggg aggcgacggc tgccatcgac
     4741 gaggtgctca acgccctggt cagctcgtac cgcgggtcgt agtggggtac gccgaccccc
     4801 ggggccggaa ccaccggatg gccacaaggg ccaagtagcg acgcgggaac ggccgtggat
     4861 ggccgccgtg ctcacgcggt gggtgtcccg gccaagatga gcgcctctcc gccctcttcc
     4921 tggccgttgg agccgccacg tggccattac ctcggcttcc tggccgttgg agccgccacg
     4981 tgcctatgac ctcggctgca cggccgctac agccacgacc ctccccgccg gagccaccag
     5041 gtggtcacaa cggccaagga gcgatgcggt aacggccgtg ggtggccgcc gtgctcacgc
     5101 ggtgggtgtc ccggccaaga tgagcgcctc tccgccctct tcctggccgt tggagccgcc
     5161 acatggcc
//
LOCUS       DATACA010000169         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2433373, whole genome shotgun sequence.
ACCESSION   DATACA010000169 DATACA010000000
VERSION     DATACA010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433373"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VHK28_03490"
     CDS             complement(<1..1395)
                     /locus_tag="VHK28_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEX2141330.1"
                     /translation="MEGQPVRSRTRTPATPAIAPGTAPTALILPPATPTPGRARPAGP
                     LRREGPAKLNGTAKYADDLVFPGAWYGATIRSTEPHARLLGIELDDAFDWKRVVVVTA
                     DDIPGTNVVSLIDDDQPVLVPIGGEIRHQAEPCALVAGPDRATVREARRHMTLRTERL
                     PAVFDPLHSEQEFANYVIERGDAAAAMERAELVIEGTYRVGHQEQLYIENQAMIAVPR
                     EDGGMTVHGSLQCPYYIHKAMKRALDLDDLQTQVIQSETGGGFGGKEEYPSIIALHAS
                     LLALKCGKPVRMIYDRHEDISATTKRHPAVIRHRTGVTRDGELVAQEVEVIMDGGAYC
                     TLTPVVLSRGSLHAAGPYRCENVRIVGRAMATNTPPNGAFRGFGAPQTEFAVETQVNR
                     IADMLDLSPLELRRRWAYREGDITPTGQVLRESVAALDVLETVAEASGFERHRQQTRE
                     ARQRRTDGARLASGI"
     gene            complement(1457..1867)
                     /locus_tag="VHK28_03495"
     CDS             complement(1457..1867)
                     /locus_tag="VHK28_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141331.1"
                     /translation="MHRRRVQHLLAAAAALGVLAGCGAPAGAGGRTLTLETLNGSGVN
                     GTVTLTAVDDETTRVLVEVDPASYPNMPAHIHPGTCAELTPQPRHPLQNVIAGRSVTD
                     VPATLDELFAATVALNLHASNEDMRTYTACVDLR"
     gene            1986..>3196
                     /locus_tag="VHK28_03500"
     CDS             1986..>3196
                     /locus_tag="VHK28_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX2141332.1"
                     /translation="MPSALLRRAIAWRPSLRVQVLAGSALLVLVLAVAIAVSVANHLR
                     ESAAEAARAHAASIVRGYIDPILGPQSLDIDAAADPEVQAQLERLAAGAEIRRINIWT
                     RDGRILYSTEKGLRGVRMDIGHELAEAFAGHAQDEFGLAADENEHVAGLPDQFLEIYV
                     PIRGTSDGSPIGVFELYQDARPIENRVENTRRDVFLITLGAGVLLFGGLWLAFGAASR
                     RLAAQNRRLSRFNEQLNSLATDLRQSEARFRSLVQNSSDVVAVLAPDGRVRYVSDALR
                     RVLGHDPAQRLERPFEEHVHHEDVGWVRQLLSGIPVRDGRQAAAELRMRHSDGSWRWV
                     ETIGQDLTGDPAVAGLVLNFRDVTDRKRLEEQLQHEAFHDPLTGLANRALFGDRVTHA
                     LARGNRGPVEQT"
BASE COUNT          430 a         1105 c         1152 g          509 t
ORIGIN      
        1 gatgccggac gccaggcgcg ccccatccgt ccgccgctgg cgcgcctctc gcgtctgctg
       61 acggtgccgt tcgaagcccg atgcctcggc gaccgtctcg agcacgtcca gcgcagccac
      121 gctctcgcgc agcacctggc cggtgggggt gatgtcgccc tcgcggtacg cccagcgtcg
      181 ccgcagctcc agcggggaga ggtcgagcat gtcggcgatg cggttgacct gggtctcgac
      241 cgcgaactcg gtctgcggcg ccccgaaccc gcgaaaggcg ccgtttggcg gcgtgttggt
      301 ggccatggcc cgccccacga tgcgcacgtt ctcgcatcga tacggcccgg cggcgtgaag
      361 gctcccccgc gagaggacca cgggcgtgag ggtgcagtac gcgccaccgt ccatgatcac
      421 ctccacctcc tgggccacca gctcgccgtc gcgggtgacg ccggtgcgat ggcggatgac
      481 ggcggggtgg cgcttggtgg tggcgctgat gtcctcgtgg cggtcgtaga tcatgcgcac
      541 cggtttgccg cacttgagcg ccagcaggga ggcgtggagg gcgatgatcg acggatactc
      601 ctccttgccg ccgaagccgc cgcccgtctc ggactggatc acctgtgttt ggaggtcgtc
      661 caggtccagc gcccgcttca tggccttgtg gatgtagtac gggcactgca gcgagccgtg
      721 gacggtcatg ccgccgtcct ctcgcgggac ggcgatcatg gcctggttct cgatgtacag
      781 ctgctcctgg tgcccgaccc ggtacgtccc ctcgatcacc agctccgccc gctccatggc
      841 cgccgccgcg tcgccgcgct caatgacgta attcgcgaac tcctgctcgc tgtgcagcgg
      901 gtcgaagacc gccggcaacc gctccgtccg cagcgtcatg tgacgccggg cctcgcgcac
      961 ggtggcgcgg tcgggtccgg cgaccagggc gcagggctcg gcctggtggc ggatctcgcc
     1021 gccgataggc acgagcaccg gttggtcgtc gtcgatgagg ctgaccacgt tggtgcccgg
     1081 gatgtcgtcc gcggttacga ccaccacccg cttccagtcg aaggcgtcat ccagctcgat
     1141 gcccagcagg cgggcgtgcg gctctgtgga gcggatggtg gcgccatacc aggcgccggg
     1201 gaagaccagg tcgtccgcgt acttcgccgt gccgttgagc ttggccggac cctcgcggcg
     1261 cagcggaccg gccggccggg cacgtcctgg cgtcggcgtg gcgggcggga ggatcagcgc
     1321 ggtgggagcg gtcccgggcg caatcgccgg ggtggcgggc gtccgggttc gggaccgcac
     1381 gggttgccct tccatcggcg aatcctagcg cggcatgggt cccacgtcag ggcgccggcc
     1441 acacgaccgg gcttgatcag cggaggtcga cgcaggccgt gtaggtgcgc atgtcctcgt
     1501 tcgaggcgtg caggttgagc gcaaccgtgg cggcgaacag ctcgtccagc gtggcgggaa
     1561 cgtcggtgac cgagcggccg gcgatcacgt tctgcagcgg gtggcgcggc tgcggtgtga
     1621 gttcggcgca cgtcccaggg tggatgtgtg ccggcatgtt cgggtagctg gccggatcga
     1681 cctcgaccag gacgcgggtg gtctcgtcgt cgaccgcggt cagggtgacc gtcccgttga
     1741 ctcccgagcc gttcagcgtc tccagggtga gggttcgccc tcccgctcca gcgggcgcgc
     1801 cgcagccggc aagcacgccg agggctgccg ccgctgccag gagatgttgc acgcgtcgcc
     1861 tgtgcatctc gaagagatgg tgtcgtcgcg caccgcctga ccaccatggg actatggtcc
     1921 ccggcggccc tggtagtacc accgtgcgct ggtgccggcc agctgcgtgc gccacgctgc
     1981 cctccgtgcc ttccgccctt ctcagacgag cgatcgcgtg gcggccgagc ctgcgcgtcc
     2041 aggtcctggc cggcagcgcc ctgctcgtcc tggtcctcgc ggttgcgatc gcggtcagcg
     2101 tcgccaatca cctccgcgaa tccgccgccg aagccgccag ggcccatgcc gcttcgatcg
     2161 tgcgtggcta catcgacccg attctcggac cgcagagcct ggatatcgac gcggctgccg
     2221 acccggaggt ccaggcacag cttgagcggc tggcggccgg cgccgagatt cgccggatca
     2281 acatctggac gcgtgacggc cggatcctgt actcgaccga gaagggcctg cgaggggttc
     2341 gcatggacat cggtcacgag ctggcggagg cattcgccgg ccacgcacag gatgagttcg
     2401 ggctggcagc ggacgaaaac gagcacgtgg ccgggctgcc ggaccagttc ctggagatct
     2461 acgtgccgat ccgcggcacg tccgacggca gccccatcgg tgtcttcgag ctctaccagg
     2521 acgcacgccc gatcgagaac cgcgtggaga acacccggcg cgacgtcttc ctgatcaccc
     2581 tgggcgccgg cgtgctgctg ttcggagggc tctggctggc gttcggggct gcctcccggc
     2641 ggctggcggc gcagaaccgc cgcctctccc ggttcaacga gcagctgaac tcgctggcca
     2701 ccgacctgag gcagagcgag gcgcgcttcc gctccctagt gcagaactcg tcggacgtcg
     2761 tggccgtgct ggccccggac ggtcgcgtcc ggtacgtgtc ggacgccctg cgccgcgtgc
     2821 tcggacacga cccggctcag cggcttgagc gtcctttcga ggagcacgtg caccacgagg
     2881 acgtcggctg ggtgcgtcag ctgctctccg gaataccggt tcgcgacggg cgccaggcgg
     2941 ccgccgagct ccggatgcgg catagcgatg gcagctggcg ctgggtagag accatcggcc
     3001 aggacctgac cggcgacccg gccgtggcgg gcctggtcct caacttccgc gacgtgacgg
     3061 accgcaagcg gctggaggag cagctgcagc acgaggcatt ccacgatccc ctcaccggcc
     3121 tggccaatcg cgccctgttc ggcgaccgcg tgacgcacgc gctggcgcgc ggcaaccgtg
     3181 gtccggtcga gcagac
//
LOCUS       DATACA010000170         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_746438, whole genome shotgun sequence.
ACCESSION   DATACA010000170 DATACA010000000
VERSION     DATACA010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746438"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VHK28_03505"
     CDS             <1..552
                     /locus_tag="VHK28_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141333.1"
                     /translation="GWFGVPAGNFYAWLFVTVGFSVVTRWLREAAARRRALDWLQLLV
                     PLPAFGLLLAGLLPFIWLKPLTDDSPGGGLALFALTLAAFCAISAWAVWGPDRGTPDG
                     QRMAIVDLRVAFATRLAIHLFFLGGLFALRLHAELPVLLGVALVLLAAELPLAALVHR
                     RMAVSGQRAERDSVGALAPARYR"
     gene            557..1945
                     /locus_tag="VHK28_03510"
     CDS             557..1945
                     /locus_tag="VHK28_03510"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2141334.1"
                     /translation="MQDHSRAVSECVEHLRALIRIPSVNPPDKADGAAGRDPSGGESA
                     VARYCAEVLSSAGIAAEVLEPVAGRGSCFARLPATVPNPEPPLVLLSHVDVVPVEAAS
                     WTRDPFGGELVDGVMWGRGAVDMKDMLAMELGVILELHRSGAELRRDVIFAALADEEA
                     GGVYGALHWIRERPDLFADSAGRPAAAALNAVGGYSMTIGGRRAYTIPVAEKGILWTR
                     LRATGTPSHGSMPSPDNAAIKLAAAVSRLAEARRPARVIPVVRSFFEGLGLGEVARQA
                     ERGRDEEARELLAELVDDLVVRRSLDAMLRDTVTPNVIHVGQKVNVVPGGGEAEIDVR
                     TLPGTDQAAFLAYLQEVAGDEVTVESVHSMPAVECAPDAEIVQLMESALRAADPEATP
                     LPMMITPGTDAKGLALLGIPTYGFAPVRLEPDVPFLSLFHANDERLPVSALEFGLPVL
                     YDVVRRYCRLED"
     gene            2077..2778
                     /locus_tag="VHK28_03515"
     CDS             2077..2778
                     /locus_tag="VHK28_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141335.1"
                     /translation="MDVDLDELRQALLGEPPYPSGWEAQVEEIMETVRTALDASRLPD
                     MTGMDPVQASCAAWRPMVGNTLWANGAFLERRLFIAHLTALAGVAPDEIRPAVEEALH
                     VSAEAAAEQMRPDGDQAVISRAPKDEIRTIGLWAVEHCDLPVVPEEMSHATDWTDEQI
                     AQSCESDRRRLEDGQEEYRSGPGEGRYALHPHALEVTLEIPLYPLWHQFADVDNDANP
                     PTFEVEPIPGGFCDV"
     gene            2854..>3821
                     /gene="fabD"
                     /locus_tag="VHK28_03520"
     CDS             2854..>3821
                     /gene="fabD"
                     /locus_tag="VHK28_03520"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2141336.1"
                     /translation="MTDAAEQQPPPRTEAAPGEPIQRIHAFVFPGQGSQYVGMGSAVV
                     QRSPAAAAVMQRADAALGFPLSQLIAEGPEDELNLTVNAQPAILATSIAYLEAMRAEA
                     AEAGIELHPLMLAGHSAGQYAAAVAAEAIDFETAMRLVRERGRIMQERGIEGGMGAVI
                     GLSDEQVDEVVESARQHGEISVANANAPGQIVLSGVIPALVFALEMSKTVGARRAVQL
                     TVSVASHSPLMRRARDEFGRILSHVPFRDPKIPMLGNVHATVIQTADGLRRELTEHLV
                     HGVQWTGTVRRMAADGVTDFVEIGPGRVLTGLIRRIAPGATAHAVDA"
BASE COUNT          503 a         1348 c         1390 g          580 t
ORIGIN      
        1 ggctggttcg gcgttccggc cggcaacttc tacgcctggc tgttcgtgac ggtaggtttc
       61 agcgtggtca ccaggtggct gcgcgaggcg gcggcgcggc gccgtgccct cgactggctg
      121 cagctgctgg tcccgcttcc ggccttcggg ctgctgctcg ccggcctgct gccgttcatc
      181 tggctcaagc ccctgaccga tgactcaccg ggcggtggcc tggcgctctt cgccctgacg
      241 ctggccgcct tctgcgcgat ctcggcctgg gccgtctggg gtcccgaccg cgggacgccc
      301 gacgggcaac ggatggccat cgtcgacctg cgggtcgcct tcgccacccg gctggccatc
      361 cacctcttct tcctgggcgg cctgttcgcc ctgcggctgc acgcggagct gccggtgctc
      421 ctgggcgtgg ccctcgtcct gctggctgcc gagctgccgc tggccgcgct cgtccatcgc
      481 aggatggctg tctctgggca gcgcgcagag cgtgattcgg tgggagcgct ggctccggcc
      541 cgctaccgtt aggcccgtgc aggaccattc ccgagccgtc tccgagtgcg tggagcacct
      601 gcgagcgctg atccgcatcc cgagcgtcaa cccgccggac aaggccgacg gcgcggcggg
      661 acgcgatccg tctggagggg agagcgcggt cgcccgctac tgcgcggagg tgctgtcgtc
      721 ggcggggatc gcggccgagg tgctggagcc tgtcgcgggc cgcggctcgt gcttcgcccg
      781 actgcccgcc accgttccga acccggagcc accactcgtc ctgctctcgc acgtggacgt
      841 cgtcccggtc gaggccgcgt cctggacgcg agacccgttc ggcggggagc tggtcgacgg
      901 cgtgatgtgg ggccggggag cggtcgacat gaaggacatg ctggccatgg agctgggcgt
      961 gattctggag ctgcatcggt caggggccga gcttcggcgc gacgtcatct tcgccgccct
     1021 ggcggacgaa gaggccggcg gcgtgtatgg ggcgctgcac tggatccggg agcgtccgga
     1081 cctgttcgcc gattcggccg gccgtcccgc cgcggccgcc ctcaacgcgg tgggcggcta
     1141 ctcgatgacc attggcgggc gccgtgcgta caccatcccg gtggccgaga agggcatcct
     1201 ctggacgcgg ctgcgggcca ccggaacgcc cagccacggc tcgatgccct cgccggacaa
     1261 cgccgcgatc aagctggccg ccgccgtcag ccggctggcc gaggctcgcc gccccgcgcg
     1321 cgtgatccca gtcgtgcgca gcttcttcga ggggctcggc cttggcgagg tggccaggca
     1381 ggccgagcgg ggccgcgacg aggaagcgcg ggagctgctt gccgagctgg tggatgatct
     1441 ggtcgtgcgc cgctcgttgg acgcgatgct gcgcgacacg gtgaccccca acgtgatcca
     1501 cgttgggcag aaggtgaacg tggtgccggg tggcggcgag gccgagatcg acgtccgcac
     1561 cctgcccggc accgaccagg cggccttcct tgcctacctg caggaggtgg ccggcgacga
     1621 ggtgacggtc gaatcggtcc acagcatgcc ggcggtcgaa tgcgcgccgg acgccgagat
     1681 cgtgcagctc atggagtccg cgctgcgggc ggccgacccg gaggcgactc cccttccgat
     1741 gatgatcacc cccgggaccg atgccaaggg cctggccctg ctcggcatcc cgacctatgg
     1801 cttcgcgccc gtgcggctgg agcccgacgt gccattcctg tccctcttcc acgccaacga
     1861 cgagcggctg ccggtctcgg ccctcgagtt cgggctgccg gtgctgtacg acgtcgtgcg
     1921 gcgctattgc cgcttggagg actgagcggt gcgcatcgcg ggcatgctga tcgcggtgct
     1981 gctgatcctg tccgcctgcc gcctggccgg tcagggcctg gcgacgccga ccgcggaggc
     2041 gacgcccaca gccaccgggt cccggtcgag gacgtggtgg acgtggacct cgacgagctg
     2101 cgccaggccc tgctgggcga gccgccctac ccgtccggct gggaagcaca ggtcgaagag
     2161 atcatggaga cggtgcgaac ggcgctcgat gcgtctcgcc tgccggacat gacgggcatg
     2221 gaccccgtcc aggcctcgtg tgccgcgtgg cgcccgatgg tcgggaacac gttgtgggcc
     2281 aacggcgcct tcctggagcg gcggctgttc atcgcgcacc tcacggctct ggcgggcgtg
     2341 gcgcccgacg agatccgccc ggcggtggag gaggcgctgc acgtctcggc cgaggccgcg
     2401 gccgagcaga tgcggccgga cggcgaccag gcggtcatct cgcgtgcccc gaaggacgag
     2461 attcgcacga tcggcctgtg ggcggttgag cactgcgacc tgccggtggt gcccgaggag
     2521 atgtcacacg ccaccgactg gaccgatgag cagatcgccc agtcgtgcga gagcgaccgg
     2581 cggcggttgg aggacgggca ggaggagtat cggtccggcc cgggcgaggg ccgctatgcc
     2641 ctccacccgc acgccctgga ggtgaccctg gagatccccc tgtacccgct gtggcaccag
     2701 ttcgccgacg tcgacaacga cgcgaacccg cccacgttcg aggtggagcc gatccccggc
     2761 ggcttctgcg acgtctgaac ttggcgacgt gggcccgatt cctgctcggg ctatactcgg
     2821 gcagttttca acgtgaccgt agttgaccct ccgatgaccg acgcagccga acagcagcct
     2881 ccgcctcgga ccgaggccgc gccaggcgag ccgatccagc gcatccacgc cttcgtcttc
     2941 ccggggcagg gttcccagta cgtgggcatg ggcagcgccg tggtacagcg ctcgcccgcc
     3001 gcggccgcgg tgatgcagcg cgccgacgcc gcgctcggct tccctctcag ccagctcatc
     3061 gccgaggggc cggaggacga gctgaacctg accgtgaacg cccagccggc gatcctggcg
     3121 accagcatcg cctacctgga ggccatgcga gccgaggcgg ccgaggccgg catcgagctg
     3181 cacccgctga tgctggccgg acactccgcc ggccagtacg ccgcggcggt cgcggccgag
     3241 gcgatcgatt tcgagaccgc catgcgcctg gtgcgggagc gcggcaggat catgcaggag
     3301 cgcggcatcg agggcggcat gggcgcggtg atcggccttt cggacgagca ggtcgacgag
     3361 gtcgtggagt cggcgcgcca gcacggcgag atcagcgtgg cgaacgccaa cgctcccggg
     3421 cagatcgtgc tctccggcgt catcccggcg ctcgtctttg cgctcgagat gagcaagacc
     3481 gtcggggccc gccgggcggt gcagctcacg gtcagcgtcg ccagccactc cccgttgatg
     3541 cgccgcgccc gcgatgagtt cggccgcatc ctgtcccacg tcccgttccg tgaccccaag
     3601 atccccatgc tcggcaacgt ccacgccacc gtgatccaga cggcggatgg cctgcgtcgc
     3661 gagctcaccg agcacctcgt acacggcgtg cagtggaccg gcaccgtgcg ccgcatggcg
     3721 gcggacggcg tgaccgactt cgtggagatc ggccccggac gcgtgctgac cgggctcatc
     3781 cggcgcatcg cacccggcgc aacggcccac gcggtggacg c
//
LOCUS       DATACA010000171         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1389283, whole genome shotgun sequence.
ACCESSION   DATACA010000171 DATACA010000000
VERSION     DATACA010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389283"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1484
                     /locus_tag="VHK28_03525"
     CDS             276..1484
                     /locus_tag="VHK28_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141337.1"
                     /translation="MTRTAQQITAMARQPAGTSLLAGAVATLVYLITAWAGAEPLDLY
                     LALADAFLHGRADVPAHPGAELAPIGATWYVPFPPAPALVYMPFVAALGTSPGVALVI
                     PPLAGGLSVGLAHLLLRRLDVALQPALWLTAGFATTTLWWVVSEGGTWHAAQVLGVLF
                     ALGALHVAISGRLALLAGVLLGLAAASRLPIGLSLPLFAYLYRRSYAAVAALLVGVAL
                     IAVPVGMYNIARFGSPIDFGYALIPSYYHPESTVLAESWFRDGIISLTYIPRSLGVMF
                     LSGFQVVPDAPYLRPSVSGLSIVLSAPVYLLAVRAPVSRLMIVSWVAVALVMLPNLAH
                     GSWGFWQLGYRFLLDATPFLLVLLGLAYRERIGPWGQAAVVLGAAVTGYGLWAMKLAD
                     FVDRALPFAA"
BASE COUNT          198 a          538 c          471 g          294 t
ORIGIN      
        1 aagagtgcta atgaactgaa gcatcctcgc gattacctcc tttcctcaag aatttgacgg
       61 cagcgtaggt tgcctgatca gggcatgaaa tgacccgatg gtcccaacta ccgccgttgg
      121 gctccgtacc atctatcgtc cggtctatta ttgtcaatag ccctcgaacc aagcgctggg
      181 cagcaaaccg caccagattg cacccatctg cggcggctcc caacagtcga aatcccgcca
      241 tccagcgcgg ccgccgatgc cacgattggc gctcagtgac acgcaccgcc cagcagatca
      301 cggccatggc ccggcaaccg gcaggcactt ccctgctggc cggagcggtg gccacgctgg
      361 tctacctcat cacggcctgg gccggcgcag agccgcttga cctctacctg gcactggccg
      421 atgccttcct acacggccgt gcggacgttc cggcgcatcc gggggcggag ctggccccca
      481 tcggagccac gtggtacgtc ccgttcccgc cggctccggc actggtgtac atgccattcg
      541 tcgccgctct tggcacgtcg ccgggcgtcg ctctcgtgat cccacccttg gccggcggtc
      601 tgtcggtcgg gctggcccac ctgctcctgc ggcggctcga cgttgcgctg cagccggccc
      661 tgtggctgac ggctggattc gccaccacca ccctgtggtg ggttgtcagc gagggcggga
      721 cctggcacgc ggcccaggtg ctcggcgtcc tgttcgccct gggcgccctg cacgtggcca
      781 tctccggacg cctcgcgctc ctggccggcg tgctgctcgg cctggcggcc gcctcacggc
      841 tccccatagg cctttcgctt ccactgttcg cctacctgta ccgccgctcc tacgccgcgg
      901 tcgcggcgct gctggtcggc gtggcgctga tcgcggtccc ggtggggatg tacaacatcg
      961 cccgcttcgg gtcacccatc gacttcggat atgccctgat ccccagctac taccatcccg
     1021 agtcgacggt cctggccgag tcgtggttcc gggacggcat catcagcctt acctatatcc
     1081 cgcgatcgct cggagtgatg ttcctgtccg gcttccaggt ggtgccggat gcgccgtacc
     1141 tgcgaccatc ggtctccggg ctgtcgatcg tgctctcggc accggtctac ctgctggcag
     1201 tgcgcgcgcc tgtcagccgc ctgatgatcg tcagctgggt ggcggtcgca ctggtcatgc
     1261 tcccgaacct ggcccacggc tcgtgggggt tctggcagct cggctatcgg ttcctgctgg
     1321 acgccacgcc gttcctgctg gtgctgctgg ggctggcgta ccgcgaacgc atcggtccat
     1381 ggggacaggc cgcggtggtg ctgggagccg ccgtcaccgg ctacgggctg tgggccatga
     1441 agctggccga cttcgtcgat cgcgcgctgc ccttcgccgc ctgatcgccg gacacaaaat
     1501 g
//
LOCUS       DATACA010000172         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1790738, whole genome shotgun sequence.
ACCESSION   DATACA010000172 DATACA010000000
VERSION     DATACA010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1790738"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VHK28_03530"
     CDS             complement(<1..787)
                     /locus_tag="VHK28_03530"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX2141338.1"
                     /translation="MDRSTAVDGGAARLDHIARRAERVLRLAGDSRLALAMLLAAGAA
                     NALAAALPDGGRLLDSPAYLVLLGAVLLSGMAAVAVRLPATWREWRAPGPPPDGEGSL
                     SVEITLEGGFDPATREALRRELAAAGYRVVERTQRGRWMLAGVRRGWSRFAGLGSHLA
                     LVLLVLGAAIGAAFSSETSFSLLPGEQALLDAPRAGFTDAVRLDTFDAEFGPDGRPLR
                     LDTAVTILRDGRAVRSQTIEVNRPGQFGGYLVHGWTYGPAASLR"
     gene            complement(799..>1918)
                     /locus_tag="VHK28_03535"
     CDS             complement(799..>1918)
                     /locus_tag="VHK28_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2141339.1"
                     /translation="VNVPIYQTSTFEVGSAEELAELLEFARHGHSYSRYSNPTHAALE
                     EALAELEGAEACHVTGSGMAAIHGVAISVLRTGDEMLIPRAVYGGVIGLAHAILQPAG
                     ISVRAVDTADLDAVAEAIGPRTRLVWIETISNPTTALADVAAIARLAHERDAAVGVDN
                     TFASPYLANPLASGADFVVHSTTKYVGGHSDLTGGAILGSADRVSAARSVVINAGGNA
                     APLEAFLALRGLKTLALRMERHSANALQVARALEGAAGITSVLYPGLESHPQHALART
                     TLREGMGGGMLAIDLEGGRGAGERFLERLRVAVHATSLGSAETLCSHPASSSHRQLSE
                     PELAEAGLTSGMVRISIGLEDPDDLVADLSAAAGASSA"
BASE COUNT          282 a          704 c          697 g          235 t
ORIGIN      
        1 cccgcagcga ggccgccggt ccataggtcc agccgtggac caagtagccg ccgaactggc
       61 ccggacggtt gacctcgatc gtctggctgc gaacggcgcg gccgtcgcgc aggatggtga
      121 cggcggtatc caggcgcagc ggccggccat cggggccgaa ctcggcgtcg aaggtatcca
      181 gtcgcaccgc gtcggtgaag ccggcgcgtg gcgcatcgag cagtgcctgc tcgccgggca
      241 gcaggctgaa gctggtctcg gacgagaacg cagcgccgat ggcggcgccc aggacgagca
      301 gcaccagcgc caggtggctg ccaagcccgg cgaagcgaga ccagccccgg cgtaccccgg
      361 ccagcatcca ccggccgcgc tgggtgcgct ccaccacccg gtagccggcg gccgccagct
      421 ccctgcgcag cgcctcccgc gtcgccgggt caaatccccc ctcgagcgtg atctcaacgc
      481 tgagcgagcc ctcgccgtcc ggtgggggcc cgggcgcacg ccactccctc cacgtggccg
      541 gaaggcgaac ggcgaccgcc gccatgccgc tcagcaggac ggcacccaac agcaccaggt
      601 aggcggggct gtcgaggagg cggccgccat cgggcagggc agccgccagt gcgttcgccg
      661 caccggcggc cagcaacatg gccagggcca ggcgcgagtc gccggccagg cgcagcacgc
      721 gctcggcgcg ccgcgcgatg tgatccaggc gggcggcccc gccatccacc gcagtcgatc
      781 ggtccatgtg ccgcccgctc aggcggacga ggcgcccgcg gcagccgaga ggtcagcgac
      841 gagatcgtcg gggtcctcga ggccaatgga gatgcgcacc atgccgctgg tcagccccgc
      901 ctcggccagc tccggctcgg aaagctgccg gtgcgacgag ctggccggat ggctgcacag
      961 cgtctccgcg ctgcccaggc tggtggcgtg aacggcgact cgaagccgct ccaggaaacg
     1021 ctccccggcc ccacgccctc cttccagatc gatggccagc atgccgccac ccatgccctc
     1081 gcgcagggtc gtccgggcca gggcgtgctg ggggtgcgac tcgaggccgg ggtacagcac
     1141 gctggtgatg ccggcagcac cctccagcgc acgcgccacc tgcagggcgt tcgcggagtg
     1201 gcgctccatg cgcagggcca gtgtcttcag gccgcgcagc gccaggaagg cctccagcgg
     1261 cgccgcgttc ccgccggcgt tgatcaccac cgagcgcgcc gccgacaccc ggtcggcgct
     1321 gcccaggatc gcgccgccgg tcaggtcgga gtggccgccg acgtacttgg tggtcgagtg
     1381 cacgacgaag tcggcgccgc tcgccagcgg gttggccagg tacggcgagg cgaaggtgtt
     1441 gtccaccccc accgccgcgt cgcgctcgtg cgccagccgg gcgatggccg ccacgtcggc
     1501 cagcgcggtg gtggggttgc tgatggtctc gatccacacc agccgggtac gcggaccgat
     1561 ggcctcggcc accgcgtcca ggtcggcggt gtcaacggcc cgcaccgaga tgccggcagg
     1621 ctgcaggatg gcgtgcgcca ggccgatgac cccgccgtac accgcgcgcg ggatgagcat
     1681 ctcgtcaccg gtacgaagga cggatatggc cacgccgtgg atggcggcca tgccggaacc
     1741 ggtgacgtgg caggcctccg cgccctccag ctcggccaat gcctcctcca gcgccgcatg
     1801 cgtcgggttg gagtagcggc tgtaggagtg gccgtggcgg gcgaactcca gcagctcggc
     1861 gagttcctcg gccgatccga cctcgaaagt gctggtctgg tagatcggca cgttgacc
//
LOCUS       DATACA010000173         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2112035, whole genome shotgun sequence.
ACCESSION   DATACA010000173 DATACA010000000
VERSION     DATACA010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112035"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VHK28_03540"
     CDS             <1..232
                     /locus_tag="VHK28_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141340.1"
                     /translation="AFVNAVMAAVLVAIALMEWQMAMVPSVVVGAVAFVIAIGLQMAF
                     NAYSARSYQREHGRRDPAGDVTSSNAPPSPSR"
     gene            334..1470
                     /gene="dnaJ"
                     /locus_tag="VHK28_03545"
     CDS             334..1470
                     /gene="dnaJ"
                     /locus_tag="VHK28_03545"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX2141341.1"
                     /translation="MAIKRDYYEILGVPRDASDEEIKRAFRRSAQRHHPDVDKSDGAE
                     ERFKELNEAYRVLGDRQRRTAYDMFGHAGVDAASAGSGGGFEGGFGGFGPFGDIFDAF
                     FGGSPAGTRQRRRVVAGADLRYDLTIEFAEAVSGVTREISFPTLATCDVCAGSGGEPG
                     TQPETCPECNGTGERRRVSQTILGQMVNITACPRCNGEGRIIATPCHNCGGDGRVRQE
                     RTLEVTIPAGIDSGQRIALEGQGEAGPRGGPPGDLYVVVAVKEHPQLVRRGTEVYHEL
                     PVTFAQAALGATLTVPTVEGSEELEIPAGTQSGTEIRLRGRGVPRLRGGGRGDLHVIV
                     TVVVPQKLTKKERDLLKQLGDVSQPAVLPTRGESFFDRLRDMFS"
     gene            1475..2386
                     /locus_tag="VHK28_03550"
     CDS             1475..2386
                     /locus_tag="VHK28_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX2141342.1"
                     /translation="MARWLELTVETDVEAVEAVSEILGRVATGSAVRPTRLIRDPADE
                     LAVREDPDAPYEVTAHIPDDLAAREAIDATERALWHLQAFDLRAVGELRVRAVDEADW
                     LERWKDGYAAQRIGRVVVVPSWLDEPIRPDEVAVRLDPGMAFGTGLHPTTRGCLTLLQ
                     RSGPMPERVLDVGCGSGILALAALRLGASRANCFDTDATAVAATLANAAANDLADRLT
                     VEQGSLPMRPVNEPYPLVLANLVAAVLVRLAGPLAAHVAPSGTLVAGGIFAERAAEVL
                     AALADAGFATAERLDDGEWVSLRMVRG"
     gene            2383..>2711
                     /locus_tag="VHK28_03555"
     CDS             2383..>2711
                     /locus_tag="VHK28_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HEX2141343.1"
                     /translation="MTPHRFFVRPDEMAGSRFPLPDQIEYQVLRVLRMRDGDRLVLLD
                     GRGEQTTVRLQGSECVVEERSPAGGEPVHRLTVWQALLRGDHLEPVVRHGTEVGVSAF
                     RLFVSER"
BASE COUNT          377 a          940 c          995 g          399 t
ORIGIN      
        1 cgcgttcgtc aacgcggtga tggcggcggt cctggtggcc atcgcgctca tggagtggca
       61 gatggccatg gtgccctcgg tggtcgtggg cgccgtggcc ttcgtcatcg ccatcggtct
      121 gcagatggcc ttcaacgcct acagcgcgcg cagctaccaa cgcgagcacg gacggcggga
      181 tccggcagga gacgtcacca gcagcaacgc gccaccgtcg ccgagccgct gacaattttc
      241 gcttcaccca cccccttgcg ccaagcacga tcacgttatc cacacgactg gcactctgaa
      301 cattagagtg ccagtttgta taatcccgcg gcgatggcca tcaagcgcga ctactacgag
      361 atcctcggcg tcccgcgtga cgcgtccgac gaggagatca agcgcgcctt tcggcgctcc
      421 gcgcagcgcc accaccccga cgtcgacaag tcggatggcg ccgaggagcg cttcaaggag
      481 ctcaacgagg cgtaccgcgt gctgggcgac cggcagcggc gcacggccta tgacatgttc
      541 gggcacgccg gcgtggatgc ggcgagcgcc gggtccggcg gcggcttcga gggcggcttc
      601 ggcggcttcg ggccgttcgg cgacatcttc gacgccttct tcggcggttc tccggccggc
      661 acgcgccagc gccgtcgggt ggtggctggc gccgacctgc gctacgacct gaccatcgag
      721 tttgccgagg ccgtgtccgg cgtgaccagg gagatcagct tccctaccct ggcgacctgt
      781 gacgtctgcg caggcagcgg tggcgagccc ggcacgcagc ctgagacctg cccggaatgc
      841 aacggcaccg gcgagcggcg gcgggtgagc cagacgatcc tgggccagat ggtcaacatc
      901 accgcctgcc cgcgctgcaa cggcgagggc cggatcatcg ccacgccgtg ccacaactgc
      961 ggcggcgacg gccgggtgcg gcaggagcgg acgctggagg tgaccatccc ggccggcatc
     1021 gacagcggcc agcgcatcgc gctcgagggc cagggcgagg ccgggccgcg gggcggcccg
     1081 ccgggtgacc tctacgtggt ggtggccgtc aaggagcacc cgcagctcgt ccgtcgcggc
     1141 accgaggtgt accacgagct gccggtcacc ttcgcgcagg ctgcgctggg tgccaccctg
     1201 acggtgccca ccgtcgaggg atcggaggag ctcgagatcc cggccgggac gcagtcggga
     1261 accgagattc ggctgcgcgg ccggggcgtg ccgcggttgc gcggcggcgg gcgcggcgac
     1321 ctgcacgtga tcgtgaccgt ggtcgtcccc cagaagctca ccaagaagga gcgcgacctc
     1381 ctcaagcagc tgggcgacgt gagccagccg gcggtgctgc ccacccgggg cgagagcttc
     1441 ttcgaccgcc tgcgcgacat gttcagctag cgccatggcg cgctggctgg agctcaccgt
     1501 cgagaccgat gtggaggcgg tcgaggccgt cagcgagatc ctcggccggg tggctaccgg
     1561 gtccgcggtg cgacccaccc gcctcatccg cgacccggcc gacgagctcg ccgtgcgcga
     1621 ggatccggac gctccgtacg aggtgaccgc ccacatcccc gacgatttgg ctgcgcgcga
     1681 agccatcgac gccaccgagc gcgcgctctg gcacctccag gccttcgacc tccgcgcggt
     1741 gggcgagctc cgcgtgcgcg ccgtcgacga ggccgactgg ctggagcgct ggaaggacgg
     1801 ctacgccgcc cagcgcattg gtcgggtggt ggtcgttccc tcctggctgg acgagcccat
     1861 ccggccggac gaagtcgccg tgcgcctgga tccaggcatg gccttcggga ccggcctgca
     1921 cccgaccacc cggggctgcc tgacgctcct gcagcgcagt ggaccgatgc cggagcgcgt
     1981 gctggacgtc ggctgcggtt cgggcatcct ggccctggcc gccctgcgcc tgggcgcgag
     2041 ccgggcgaac tgctttgaca ccgatgcgac cgcggtggcg gcgacgctgg cgaatgcggc
     2101 cgccaacgac ctggccgatc gcctgaccgt cgagcagggc agcctaccta tgcggccggt
     2161 gaacgagccc tacccgctgg tgctggccaa cctggtggcg gccgtgctgg tccggctggc
     2221 agggccgctg gcggcgcacg tcgcaccgtc cggcacgctg gtagccggtg gcatcttcgc
     2281 cgagcgggcg gcggaggtgc tggcagcgct cgccgacgcc ggcttcgcga ccgcggagcg
     2341 cctcgacgac ggggagtggg tctcgttgcg catggtgcgc ggatgacccc tcaccgcttc
     2401 ttcgtgcggc cggacgagat ggcgggatcg cgcttcccgc tgcccgacca gatcgagtac
     2461 caggtgctgc gcgtgcttcg catgcgggac ggcgaccgtc tcgtgttgct cgacggtcgc
     2521 ggtgagcaga ccaccgtgcg gttgcagggc agcgagtgcg tggtcgagga gcgctccccg
     2581 gctggtggcg agcctgttca tcggctgacg gtgtggcagg cgctcctgcg gggagaccac
     2641 ctggaaccgg tcgtgcggca cgggactgaa gtgggcgtct ccgcgttccg cctgttcgtc
     2701 tccgagcggt g
//
LOCUS       DATACA010000174         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2352867, whole genome shotgun sequence.
ACCESSION   DATACA010000174 DATACA010000000
VERSION     DATACA010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352867"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VHK28_03560"
     CDS             <1..611
                     /locus_tag="VHK28_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2141344.1"
                     /translation="WPVYAETCPHYLSKTDAAYGAEDESEVIRSVISPPLRSEADRQA
                     LWLGVAHSGLDVVASDHVPDRLAVEKAVPAPPFPQISNGAPGIETLLAVTYSTGPGAG
                     RVSLERMVDVLSTTPARLFGLETKGTVEAGKDADLVLFDPTVSRTVTQESLHHTSDFT
                     PYEGTQLRGAVRTVLLRGEPVVREGLFVGRRGRGRFLERRLA"
     gene            697..>1721
                     /locus_tag="VHK28_03565"
     CDS             697..>1721
                     /locus_tag="VHK28_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2141345.1"
                     /translation="MTCTACGTHNEAGRKFCGECGARLLTACPSCGALNAPTVKFCGE
                     CGTPLVTEAAPIAANQPDEGTNGETTMPRPTAVAERRLVSVLFADLVGFTTLSEERDP
                     EEVRELLTRYFDTARSLVGRYGGTIEKFIGDAVMAVWGTPTTNEDDAERAVRAALDLT
                     AAVAALGEEVGIPTLRARAGVLSGEAAVTLGATGQGMVAGDLVNTASRIQSAARPGQV
                     LVGPETKHATDMAIDYEDAGSQELKGKAGAVQLWRATRVVGLTGGTVRSQEIEPPFIG
                     RDPELRLIKQLFHSSADESRAHLVSVMGIGGIGKSRLAWEFEKYIDGLAQDVWWLRGR
                     CLAYGDGV"
BASE COUNT          261 a          600 c          618 g          242 t
ORIGIN      
        1 gctggccagt ctacgcagag acctgcccgc actacctttc caagaccgat gcggcctacg
       61 gcgccgaaga cgaatcagag gtcattcgat ccgtcatctc gccgcccctt cgctcggagg
      121 cggaccgcca ggcgctgtgg ctgggggtgg cacactccgg gctggacgtc gtcgcctcgg
      181 accacgtgcc ggaccggctg gccgtggaaa aggccgttcc cgcgccgccc ttcccgcaga
      241 tcagcaacgg cgcgccggga atcgagacgc tcctggcggt gacctactcg acagggccgg
      301 gcgcggggcg ggtatcactg gagcggatgg tggacgtgct ctccacgacg ccggcgcgcc
      361 tcttcggcct ggagaccaag ggcaccgtcg aggccggcaa ggacgccgac ctggtgctct
      421 tcgacccgac tgtgtcgcgc acggtcacgc aggagagcct gcaccacacc agcgacttca
      481 cgccgtacga ggggacgcag ctgcgcggtg cggtacggac cgtcctgctc cgcggggagc
      541 ctgtggtccg cgagggcctc ttcgtggggc gccgagggcg tggccgattc ctggagcgaa
      601 ggctggcctg actccgccag acctgtaccc ggccgggccc atcgggtacc ctgcggccct
      661 gcgcgtcacg cgctcacctg acatcgcgga ggatccatga cctgcaccgc ctgcgggacg
      721 cacaacgagg cgggacgcaa gttctgcggc gagtgcggcg ccaggctgct gaccgcctgc
      781 ccctcctgcg gcgcgctgaa tgccccgacc gtcaagttct gcggcgaatg cggaacgccg
      841 ctcgtcaccg aagccgctcc gatcgccgcc aaccagcccg acgaaggcac caatggcgag
      901 acgaccatgc cgcgtcccac cgccgtggca gagcggcgac tggtctcggt cctgttcgcc
      961 gacctggtgg gcttcaccac cctcagcgag gagcgggatc cggaggaagt ccgcgagctc
     1021 ctgacgcgct acttcgacac cgcccgctcg ctggtcggtc gctacggcgg cacgatcgag
     1081 aagttcatcg gtgatgcggt catggccgtg tggggtacgc ccaccaccaa cgaggacgat
     1141 gccgagcgcg ccgttcgcgc ggcgctggac ctgactgccg ccgtggcggc gctcggcgag
     1201 gaggtgggca tccccaccct ccgcgcgcgt gcgggtgtcc tctccgggga ggccgccgtg
     1261 acgctgggcg ccaccggtca gggcatggtg gccggcgacc tggtcaacac cgcctcgcga
     1321 atccagtcgg cggcccgtcc cggccaggtc ctggtgggcc ccgagaccaa gcacgccacc
     1381 gacatggcga tcgactacga ggacgctggc agccaggagc tcaagggcaa ggcgggcgcc
     1441 gtccagctgt ggcgcgcgac ccgggtggtg ggcctcaccg gtggcacggt gcgcagccag
     1501 gagatcgaac cgccgttcat tggtcgtgac cccgagctgc ggctgatcaa gcagctcttc
     1561 cacagcagcg ccgacgagag ccgcgcgcac ctggtgtcgg tgatgggcat cggcggcatc
     1621 gggaagtcaa ggctggcctg ggagttcgag aagtacatcg acggcctggc gcaggacgtg
     1681 tggtggctgc gggggcgctg cctggcgtac ggcgacgggg t
//
LOCUS       DATACA010000175         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2032806, whole genome shotgun sequence.
ACCESSION   DATACA010000175 DATACA010000000
VERSION     DATACA010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032806"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VHK28_03570"
     CDS             complement(<1..356)
                     /locus_tag="VHK28_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX2141346.1"
                     /translation="MARGPARPGPAQGPKDIPGPTVNLQLLAFNDYHGHLQSDTPGSV
                     GSIPAGGAAFLGAHLESLRKGAKYSLTVAAGDLIGGTPFLSGLFKDEPSVESLNAIGL
                     DVSSVGNHEFDEGVTEL"
     gene            complement(568..1974)
                     /locus_tag="VHK28_03575"
     CDS             complement(568..1974)
                     /locus_tag="VHK28_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015135189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX2141347.1"
                     /translation="MELNGIQIATFFVWLVGSVTFIIALKFLASPGTARTGNLLGAAG
                     MALVIAWTFFTVEGMLENWWIIVVGGLVGSVAGVLGARRVPMTAMPQMVAIFNGAGGG
                     AAAVVAVAEFLEFAPEADGLLPLPFVIATLAGAIIGSVALTGSIIAFGKLQGIVTSRP
                     YMLPGQRLITGVLFLAMLALAVYVAAFENSIPLFLLYCALALVAGVVLVMPIGGADMP
                     VVVSLLNSYTGIAVAASGFVLSNYALLIAGTLVGASGAILTQLMTKAMNRSLVNVLFA
                     GFGTGGGPAATGAEGEEQLREITAEDAAVLMAYGKRVIIVPGYGMAVAQAQGPVRELM
                     DLLTERGVDVQFAIHPVAGRMPGHMNVLLAEANVPYDRLKEMDEINDDFADTDVALVI
                     GANDVVNPLAREEGSPISGMPILNVDQAENIIVLKRGRGTGFAGVPNPLFTNEKTTML
                     FGDARPSVEDLVAGVKQA"
     gene            complement(1975..2292)
                     /locus_tag="VHK28_03580"
     CDS             complement(1975..2292)
                     /locus_tag="VHK28_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX2141348.1"
                     /translation="MDATTLSPFALLAALFILVLAAFVGYEVVSKVPTMLHTPLMSGA
                     NAVHGVIVVGAMLVAATAEGPLRIVLALIGVTLGTINVVGGFVVTDRMLEMFRRRSPG
                     GRR"
     gene            complement(2292..>2559)
                     /locus_tag="VHK28_03585"
     CDS             complement(2292..>2559)
                     /locus_tag="VHK28_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141349.1"
                     /translation="SMPGSATQMYAKNMQTLVKHLLKDGKVELNFDDEITRGATITHQ
                     GKVVHAATAEALGIQPAEPAKAEPASVQPSQTDAPAAAGEKERA"
BASE COUNT          410 a          866 c          906 g          377 t
ORIGIN      
        1 agctcggtga cgccctcgtc gaactcgtgg ttgcccacgc tggagacgtc gagaccgatg
       61 gcgttcagcg actccaccga cggctcgtcc ttgaagagcc cggacaggaa cggcgtgccg
      121 ccgatcaggt caccggccgc caccgtgagc gagtacttgg ctcccttgcg caggctctcg
      181 aggtgggcgc cgaggaacgc cgctcctccg gccgggatcg atccgaccga gcccggagtg
      241 tctgactgca gatggccgtg gtagtcgttg aaggccagca gctggaggtt gacggtggga
      301 cccgggatgt ccttcggccc ctgggccgga cctggacggg ctggtccgcg cgccatggcc
      361 ggtgcgacca tcgcgagcag cagggcgagt gcgccgagca gggccgtcag ccgaccgggg
      421 tgagacgtgg ccaatcggag catcgtgagg caacctccaa gcagcggtcg cgctcggcgc
      481 cggggcacac atcgggcagc aggatgtcag cgctcgagag cgcggattgt tgggtcggac
      541 cagcgtactc ccgccgtcca gcgcccgcta ggcctgcttg actcctgcca ccaggtcctc
      601 gaccgagggg cgtgcgtccc cgaacagcat ggtcgtcttc tcgttggtga acagcgggtt
      661 cgggacgccg gcgaagccgg tgccccgccc acgcttgagg acgatgatgt tctcggcctg
      721 gtcgacgttg aggatgggca tgccgctgat gggcgagccc tcctcgcggg cgagcgggtt
      781 gaccacgtcg ttggcaccga tgaccagggc gacatcggtg tcggcgaagt cgtcgttgat
      841 ctcgtccatc tccttgagcc ggtcgtaggg cacgttggcc tcggccagca ggacgttcat
      901 gtggcccggc atgcggccgg ccaccggatg gatggcgaac tgcacgtcca cgccgcgctc
      961 ggtcagcagg tccatcagct cccggaccgg cccctgcgcc tgggcgaccg ccatgccgta
     1021 gccgggcacg atgatcaccc gcttgccgta ggccatcagc accgccgcgt cctcggccgt
     1081 gatctcgcgc agctgctcct cgccctcggc ccccgtagca gccgggccgc cgccggtgcc
     1141 gaagccggcg aacagcacgt tgaccagtga ccggttcatg gccttggtca tcagctgggt
     1201 caggatggcg cctgacgcgc cgaccagagt gcccgcgatc agcagggcgt agttcgacag
     1261 cacgaagccg gatgccgcca ccgcgatgcc ggtgtagctg ttcagcaggc tgaccacaac
     1321 cggcatgtcc gcgccgccga tgggcatgac cagcaccacg ccggccacca gggccagcgc
     1381 gcagtagagc aggaagaggg ggatgctgtt ctcgaaggcg gccacgtaca cggccagcgc
     1441 cagcatggcc aggaacagga cgccggtgat cagccgctgc ccgggaagca tgtaggggcg
     1501 agaggtgacg atgccctgca gcttgccgaa ggcgatgatc gagccggtca gggccaccga
     1561 gccgatgatg gcgccggcca gggtggcgat cacgaagggc agcggcagca ggccgtcagc
     1621 ctcgggggcg aactccagga actcggcgac ggccaccacc gcggccgcac cgccgccggc
     1681 gccgttgaag atggccacca tctgcggcat ggcggtcatg ggcacgcgcc gcgcgccgag
     1741 cacgccggcc accgagccga ccaaaccgcc gaccacgatg atccaccagt tctcgagcat
     1801 gccctcgacg gtgaagaagg tccaggcgat gaccagcgcc atgccggccg cgccgagcag
     1861 gttgccggtg cgagccgtgc ccgggctggc caggaacttg agggcgatga tgaaggtgac
     1921 cgacccgacc agccagacga agaaggtcgc gatctggatg ccgttgagct ccatctagcg
     1981 acgccctcct gggctgcgcc ggcggaacat ctccagcatc cggtcggtga ccacgaagcc
     2041 gccgaccacg ttgatggtgc ccagcgtgac gccgatcagc gccagcacga tgcgcagcgg
     2101 tccctccgcg gtggcggcca ccagcatggc gccgaccacg atcacgccgt gcacggcgtt
     2161 ggcgccgctc atcagcgggg tgtgcagcat ggtgggcacc ttgctgacga cctcgtaacc
     2221 gacgaaggcg gccagcacca gtatgaagag cgcggccagc agcgcgaacg ggctgagggt
     2281 cgtggcatcc atcaggctct ctccttctcg cccgccgcgg cgggcgcatc ggtctgggac
     2341 ggctggaccg atgccggctc cgccttggcc ggctccgccg gctggatgcc gagcgcctcg
     2401 gcggtcgcgg cgtgcaccac cttgccctga tgggtgatgg tcgcgccgcg cgtgatctcg
     2461 tcgtcgaagt tgagctccac cttgccgtcc ttgaggaggt gcttgaccag ggtctgcatg
     2521 ttcttggcgt acatctgggt ggcgctgccg ggcatgctg
//
LOCUS       DATACA010000176         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1469351, whole genome shotgun sequence.
ACCESSION   DATACA010000176 DATACA010000000
VERSION     DATACA010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469351"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1420)
                     /locus_tag="VHK28_03590"
     CDS             complement(191..1420)
                     /locus_tag="VHK28_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2141350.1"
                     /translation="MALDQSALLEVLDALKAADVDDRIRSAATTIYQALIEAELTAVI
                     GAGPHERTDARTGQRNGSRPRTLTTTAGDLELRIPKLRAGSFFPSLLERRRRIDQALF
                     AVVMEAYLHGVSTRKVDDLVRALGADTGISKSEVSRICADLDAEVSVFRDRSLAEQPF
                     PYVFLDATYCKARVNRRVVSQAVVIATGVAADGHREVLGFAVGDSEDGAFWTAFLRTL
                     KARGLAGTQLVISDAHTGLKQAIGAVLLGAAWQRCRVHFMRNVLARVPKGNSEMVAAA
                     IRTVFAQPDAEHVRTQLDVIAGMLGRQFPAVEAMLRDAAEDLLAFTGFPVAHWKKIWS
                     TNPLERLNKEIKRRTDVVGVFPNPAALLRLAGAVLVEAHDEWQVSDRRYLAEGSMALL
                     NQPNPAKEVAQPALIAS"
BASE COUNT          233 a          527 c          596 g          258 t
ORIGIN      
        1 tcccggtgcc cagccggcgg ctgtatcacg ggccctcgga ggccaaaggg actgcctccc
       61 ttcagttgac gctctggtgc gccccgcgtt ccgtggagtc gcttcgctgg acttcatgcc
      121 acggtgatca gggtgacgtc ccgctgagtg gtggaagtag gcctcaccgt gctggtcagc
      181 ggtgtggtga ctatgacgcg atcagcgccg gctgcgccac ctccttggct gggttcggtt
      241 ggttgagcag ggccatggag ccttcggcga ggtagcggcg gtcggatacc tgccattcgt
      301 cgtgcgcttc gaccaggacc gcgccggcga ggcggagcag ggcggcgggg ttggggaaga
      361 cgccgacgac gtcggtgcgg cgcttgatct ccttgttcag ccgttccagc gggttggtcg
      421 accagatctt cttccagtgc gcgacaggga agccggtgaa ggccagcagg tcctcggcgg
      481 cgtcacggag catggcctcc acggccggga actggcggcc gagcatgccg gcgatgacgt
      541 cgagctgggt gcgcacgtgt tcggcgtcgg gctgggcgaa gacggtgcgg atggcggcgg
      601 cgaccatctc gctgttgccc ttcgggacgc gggcgagcac gttgcgcatg aagtgcaccc
      661 ggcagcgctg ccaggccgcg ccgagcagca ccgcaccgat ggcctgcttg aggccggtgt
      721 gggcgtcgga gatgaccagc tgggtgccgg ccagcccgcg ggccttgagc gtgcgcagga
      781 aggcggtcca gaaggcgccg tcctcggagt cgccgacggc gaagcccagg acctcgcggt
      841 gcccgtcggc ggccacgccg gtggcgatca ccaccgcctg ggagaccacc cggcggttga
      901 cccgcgcctt gcagtaggtg gcgtcaagga acacgtacgg gaagggctgc tcggccagcg
      961 agcggtcgcg gaagacactg acctcggcgt cgaggtcggc gcagatccgg gacacctcgg
     1021 acttgctgat gccggtgtcg gcgcccagcg cccgcaccag gtcatcgacc ttccgcgtgg
     1081 agacgccgtg cagataggcc tccatcacca ccgcgaacag cgcctggtcg atccgccggc
     1141 gccgttccag cagcgagggg aagaacgacc cggcgcgcag cttcgggatc cgcagctcca
     1201 gatccccggc cgtcgtggtg agcgtccgcg gccgcgagcc gttgcgctgg ccggtccggg
     1261 cgtcggtgcg ctcgtgcggg ccggcgccga tcaccgcggt gagctcggcc tcgatcaagg
     1321 cctggtagat cgtggtcgcc gccgaccgaa tccggtcgtc gacatcggcg gccttcagtg
     1381 cgtcgagcac ctcgagcagg gcagactggt ccagggccat cgtgtgctgt gtccttccgc
     1441 gagaaccatt ggcgtggtct cgctgaccat cacacgatgg ccccttcgcg tggtcatcca
     1501 cgcgagcggt gacctggact tacaccacca gcggggacgt caccccggac taggagcagg
     1561 cactgtagat cgggtcgtcg cctccactga tcgagccact ggttggtggg cagg
//
LOCUS       DATACA010000177         1593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1710733, whole genome shotgun sequence.
ACCESSION   DATACA010000177 DATACA010000000
VERSION     DATACA010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710733"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VHK28_03595"
     CDS             complement(<1..432)
                     /locus_tag="VHK28_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141351.1"
                     /translation="MAGATAAKGSPPAQRRPRLLFYAMYDASVASNAPRVRIQMLAAA
                     LERRADVRLVSGGRVARLRAGAGLLAGGLRRFDAAYVEAPTTTPMPWDLLVLAAARAR
                     GLPVGIYFRDAYQLFRDLYPLRGWRARLSDLAWRLTLGPMRT"
     gene            complement(438..980)
                     /locus_tag="VHK28_03600"
     CDS             complement(438..980)
                     /locus_tag="VHK28_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX2141352.1"
                     /translation="MASDRSGGTVEVTVEIPSGSRNKYEYDHARHRFVLDRVLYSSVH
                     YPADYGAIDGSLAEDGDPLDVLVVISEPTFPGCVVRARPVGVLDMTDDKGHDYKVLAV
                     AHDDPRWDQTNALEDLPPHLLREIENFFETYKTLEGRETDVSGWLGVTDAWRIIEEAM
                     AAGREQAASGVERVRPGTWR"
     gene            complement(1162..>1593)
                     /locus_tag="VHK28_03605"
     CDS             complement(1162..>1593)
                     /locus_tag="VHK28_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2141353.1"
                     /translation="RAALEERAAGLGIGGRVIIVDAIPHRLMAEYLRLADMVVSVPES
                     DSFPVAVQEAMATGVPVVTSDLPAARAALEELAPDALVPVGDVDATATALQRAIELAP
                     DERHAIGLLLREQVVAHGDYRSNMEIMEDLYRNLVVATGRA"
BASE COUNT          222 a          579 c          564 g          228 t
ORIGIN      
        1 cgtgcgcatc ggaccgagcg tcagccgcca ggccagatcg gaaaggcggg cgcgccagcc
       61 gcgcagcggg tagaggtccc ggaacagctg gtaggcgtcg cggaagtaga tgcccaccgg
      121 caggccccgg gcacgggccg ccgccagcac cagcaggtcc cagggcatgg gcgtggtggt
      181 gggtgcctcg acgtaggccg catcgaagcg gcggaggcca ccggccagga gtccggctcc
      241 ggcgcgcagc cgtgcgaccc ggccgccgct cacgaggcgc acatcggcgc ggcgttcgag
      301 cgcggcggcg agcatctgga tccgcacccg cggcgcgttg ctggccaccg aggcgtcgta
      361 catggcgtag aacagcagcc gcggacgccg ctgggctggc ggcgagccct tcgccgcggt
      421 cgcgccggcc acctgcgtca gcgccaggtc ccgggccgga cccgctcgac gccgctggcg
      481 gcctgctcgc gcccggccgc catggcttcc tcgatgatcc gccacgcgtc ggtgacgccc
      541 agccagccgg agacatcggt ctcgcgcccc tcgagcgtct tgtaggtctc gaagaagttc
      601 tcgatctcgc gcaacaggtg gggcggcagg tcctcgagcg cgttggtctg gtcccagcgc
      661 gggtcgtcgt gggcgacggc cagcaccttg tagtcgtggc ccttgtcatc ggtcatgtcc
      721 aggacgccga ccggacgggc gcgcaccacg caccccggga aggtgggctc gctgatgacc
      781 accagcacgt ccagcgggtc gccgtcctcg gccaggctgc cgtcgattgc gccgtaatcg
      841 gccgggtaat gcacgctgct gtacagcacg cggtccagca cgaaccgatg tcgcgcgtgg
      901 tcgtactcgt acttgttgcg gctgccggac gggatctcca cggtgacctc cacggtcccg
      961 ccgctacggt cgctcgccat gcgcccgagt atgcgcgatt ctcggctcct cggcgaaacg
     1021 ccacccagcg cggggcacgc ggggttcacg tggtgagcac tacggccatc gagcggccca
     1081 gcgagtcgac ttcctggcgg ccctgctccc caggtggacc gctcggccag gaaggggccg
     1141 ttttccctgg ctcgttggcc gttatgcccg cccggtggcc acgacgaggt tccggtacag
     1201 gtcctccatg atctccatgt tggaccgata gtccccgtgt gcaaccacct gctcccgcag
     1261 gagtagaccg atggcgtggc gctcgtccgg ggcgagctcg atcgcccgct gaagcgcggt
     1321 ggcggtggcg tccacgtcgc ccaccggtac cagcgcatcc ggtgccagct cctccagtgc
     1381 agcacgcgcc gcgggaaggt ccgaggtgac caccggcacg ccggtggcca tggcctcctg
     1441 gacggccacc gggaaggagt cgctctccgg gaccgatacc accatgtccg ccaggcgcag
     1501 gtactccgcc atcaggcggt gcggaatggc gtccacgatg atgacccggc cgccgatccc
     1561 gagcccggct gcccgctcct cgagcgccgc gcg
//
LOCUS       DATACA010000178         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1872050, whole genome shotgun sequence.
ACCESSION   DATACA010000178 DATACA010000000
VERSION     DATACA010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872050"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..519)
                     /locus_tag="VHK28_03610"
     CDS             complement(34..519)
                     /locus_tag="VHK28_03610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2141354.1"
                     /translation="MELTALAARRIPAPAELIYTYIADFGQHHHRFLPDAFGDFRVEE
                     GGVGAGTVNSFTMTLGGQTRAYRTRIEEPEPGRVLTETATDGSSHTTFTVTPDGDGTR
                     VQIETTWRGAGGVAGIMERLVAPAMLRRVYAEELERLDAYARAEIKGPAVPRTAQIHT
                     N"
     gene            712..915
                     /locus_tag="VHK28_03615"
     CDS             712..915
                     /locus_tag="VHK28_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141355.1"
                     /translation="MTLILVVPMVGGTVLGLVLDAMLESSPVAVVSGLVVGSLVAATG
                     VWLYIRAHAGRIGGPDDGRADGQ"
     gene            905..1117
                     /locus_tag="VHK28_03620"
     CDS             905..1117
                     /locus_tag="VHK28_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX2141356.1"
                     /translation="MGNERPNAPWGIAFDLGLRLGISVILGLGGGLVVDNWLDTLPLF
                     TLLGTALGIGAAMYTIWDVARDAMRR"
     gene            1125..1964
                     /gene="atpB"
                     /locus_tag="VHK28_03625"
     CDS             1125..1964
                     /gene="atpB"
                     /locus_tag="VHK28_03625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX2141357.1"
                     /translation="MPEEEGGDLIHVSIEAEPLFNIGPFEFTNSMVGALLASLVLLAA
                     AWYLYRRGTLVPGRMQSLIELPIEWMTSIVSASTTRWRGYVALIIGFFLMILVANWIG
                     LLPGVGTIGLVHHEEGHTTLVPLIRPASADLNFTLGLSIVAFITFVWWGVRINGVGGY
                     LKELVGEPRYMAPLMFPIHLISELSRLISLSMRLFGNVFAGEVLLATMLALTFVGVPA
                     IFLGLEAVFGFVQALVFSLLAMTYITLSIAEHHGGGHGHEGGEEENPDVQRHPPDVAS
                     VQS"
     gene            2027..2251
                     /gene="atpE"
                     /locus_tag="VHK28_03630"
     CDS             2027..2251
                     /gene="atpE"
                     /locus_tag="VHK28_03630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX2141358.1"
                     /translation="MEGDISTLAAGLAIGLGAIGPGIGLGIAVSRAMEALGRNPDASG
                     AIFVPYILGIALTEAIGIYALVIGLLLIFV"
BASE COUNT          330 a          749 c          826 g          421 t
ORIGIN      
        1 gaacgttcgg ttgcctgttt cggccgctgc tggtcagttg gtgtgaatct gcgcggttcg
       61 cggcacggcc ggccccttaa tctccgcgcg agcgtaggcg tccaggcgct ccagctcctc
      121 ggcgtatacg cggcgcagca tggcgggggc gaccaggcgc tccatgatgc cggccacgcc
      181 gccggcgccc cgccaggtcg tctcgatctg gacgcgcgtg ccgtcgccgt cgggagtgac
      241 ggtgaaggtg gtgtggctgg agccgtcggt ggcggtctcg gtcaggacgc gccccggctc
      301 gggctcctcg atccgcgttc ggtaggcgcg ggtctggccc cccagggtca tggtgaagct
      361 gttgaccgtg ccggcgccca cgcctccctc ctcgacccgg aagtcgccga aggcgtccgg
      421 caggaaccgg tggtggtgct ggccgaaatc ggcgatgtag gtgtagatca gttcggccgg
      481 cgccgggatg cggcgagcgg cgagggcggt gagttccatg ccattcagca tgcggggcgg
      541 ccggcggcgg acgcatcggt cctgcggccg aagcggccgt acgtcgaatg accgggtggc
      601 ccgggagcga cgatcgaagg cctcggctat actcgcgccg tccgaccgct gtgagtcgaa
      661 ctccgtatga cacaactgac gcctgcggac ctcgtcagga tcctgggcca cgtgacgctc
      721 atcctggtgg tccccatggt gggaggcacg gtgctgggcc tcgtgctcga cgcgatgctc
      781 gaaagctcac cggtggcggt ggtcagcgga ctggtggtcg gaagcctggt ggcagcaacg
      841 ggagtgtggc tctacatccg cgcccacgcg gggcggatcg gagggccgga cgacgggcga
      901 gcggatgggc aatgagaggc ccaacgcgcc gtggggcatc gccttcgacc tggggttgcg
      961 gctggggatc tcggtgatcc tggggctggg cggcggcctg gtcgtggaca actggctcga
     1021 tacgctcccg ctcttcaccc tgctggggac ggcgctgggc atcggtgccg cgatgtacac
     1081 gatctgggac gttgcccgcg acgcaatgag gagatgaccg caacgtgcct gaggaagagg
     1141 gcggcgacct gatccacgtc agcatcgagg ctgagccgct cttcaacatc ggaccgttcg
     1201 aattcaccaa cagcatggtt ggcgcgctgc tggcgtcgct ggtgctgctg gccgcggcct
     1261 ggtacctgta ccgccgcggc acgctggtgc ccggccgcat gcagagcctg atcgagctgc
     1321 cgatcgagtg gatgacctcg atcgtgtcgg cctccaccac ccgctggcgc ggctacgtgg
     1381 cgctgataat cggcttcttc ttgatgatcc tggtggccaa ctggatcggg ctgttgccgg
     1441 gcgtgggcac catcggtctc gtgcaccacg aggagggcca taccacgctg gtgccgctca
     1501 tccggccggc ctcggccgac ctgaacttca cgctggggct gtcgatcgtg gccttcatca
     1561 ccttcgtgtg gtggggcgtg cgtatcaacg gcgtgggcgg ctacctcaag gagctggtcg
     1621 gcgagccgcg ctacatggcc ccgctgatgt tccccatcca cctcatcagc gagctgagtc
     1681 gcctgatcag cctgtcgatg cgactcttcg gcaacgtctt cgccggtgag gtgctgctcg
     1741 cgaccatgct ggccctcacc ttcgtcggcg tgccggccat cttcctgggg ctggaagccg
     1801 tcttcggctt cgtgcaggcg ctcgtcttca gcctgctggc catgacctac atcaccctct
     1861 ccatcgcgga gcaccatggc ggtggccacg ggcacgaggg tggcgaggag gagaacccgg
     1921 acgtccagcg gcatccgccc gatgtcgcat cggtccagag ctaggacacc aatcagggca
     1981 aatcgacgcc tgcactcgca ggcgcaacgt aggaggaatt gcttcagtgg aaggtgacat
     2041 ctccacactg gcggccggtc tggcgatcgg tctcggcgcc atcggtcccg gcatcggcct
     2101 gggcatcgcg gtgagcaggg ccatggaggc gctggggcgc aacccggacg cttccggcgc
     2161 gatcttcgtg ccgtacatcc tgggcatcgc gctgacggag gccatcggca tctacgcgct
     2221 ggtcatcggc ctgctcctga tcttcgtcta gggaggccga gcgttgctcg agttcttcaa
     2281 cgccttcggc gtcgacgccc tgaagctcgc cttccaggtc gtcaac
//
LOCUS       DATACA010000179         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1711086, whole genome shotgun sequence.
ACCESSION   DATACA010000179 DATACA010000000
VERSION     DATACA010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711086"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1462)
                     /locus_tag="VHK28_03635"
     CDS             complement(239..1462)
                     /locus_tag="VHK28_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141359.1"
                     /translation="MPSPPHPPDHAAPEDDARAAAIERIAIWGDLDPAPFAVLHREWE
                     EAQTSVRPRSRRTLDLIRRVPIGVAALWVFLVALALMKDGSRGLAPALGEAVGGAMDA
                     LGIGWLGSLLVLSGSPVAASALALFDGGFLDLDEAYGMVVGSRLGAAFVVLVVGAAYA
                     LTGRAVGRRAPISIGVLALAMTALMYVPGGIAGLLLLRSGALDSVSLTPPPVFFDLTD
                     AATAPVVGAIEGILPPESALGAAAVFALGVLLLLVSFRLVDALLPKLEAADSGGNARW
                     YVGPWTVFGIGLLVALATLSVSVALSLLVPAVSRGLVRREQILPYVAGANITTLVDTL
                     LVAVLLGNPDAPRIVLSLAIGITVVTLLTLAFAYRSTRRAVFRLQEVVLHSPARLASF
                     VAVLFAVPLLLVLIT"
     gene            1569..2939
                     /locus_tag="VHK28_03640"
     CDS             1569..2939
                     /locus_tag="VHK28_03640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX2141360.1"
                     /translation="MQITVVGLGYVGLVTATCLARLGQDVIGLEADPDKLRALERDEP
                     PFFEPGLEGEIAFQRSTGRLRFTGESAQALRTPDVVLICVGTPSRPSGEADLRAVEQV
                     IDSIAGELQNHAVIALRSTVPVGTTRKVEARLNARLGEHGGTASVPVFANPEFLRTGR
                     AIEDFLRPWRVVLGKTELPTEEDVERLLTLYRPLESPTLVFDAESAELVKNAANAYLA
                     TRISFINELSALCERTGASIDAVISGIASDPRIGADYLRPGIGYGGSCLPKDVRSLVA
                     MGDHYKVPMQLALAVDAINQQQPLRAAERLADELGGSLQGRRIGLLGLSFKPDTDDIR
                     DSPALALARALRGMGADVAACDPEAAAAAAAREPWIEIVAGPSELAARADALVLATEW
                     PEYVTLDMGPLAADMRQPILLDARNALDAKVVMAAGFRYLRVGNPAGDVTDLLAARPE
                     ASRRSA"
     gene            complement(2890..>3516)
                     /locus_tag="VHK28_03645"
     CDS             complement(2890..>3516)
                     /locus_tag="VHK28_03645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX2141361.1"
                     /translation="LAAVAGVGARLVFASSSSVYGNDDRRPFKEDQMAARPVSPYGAT
                     KVAGEALVHAHHALTGLPVMLARLFTVYGPRQRPDLAIHTFATRILRGDPIELYGSGT
                     VPRDYTYVDDVVDALVRMADTDHPRLVVNVGSDRPITNGEMLTALETALGMDAVRRDV
                     PHQPGDALATHADITRAREVLGWQPRTRFEDGIRRFVDWLRAEQPGGR"
BASE COUNT          604 a         1208 c         1234 g          470 t
ORIGIN      
        1 caacgtcccc cggatcccag cagttgccgc agacgaagtg gccgcaacgc gagcagaacg
       61 tgtagtggtc ggcctcagcg ccgcaccgat cacatccgac tccggcccgc agggtcatcg
      121 tccgcctcac cccccaaacc cagtgggaac cactcgccgc agcgcagctg ggcgaccatt
      181 ttaggccctt ggtggtcgga cgaccagagt cacgccatat cgcgcttggg gccaatgctc
      241 aggtgatcag gacgagcaga agcggcacgg caaacaggac cgccacgaac gaggccagcc
      301 gagcggggct gtgcaggacg acctcctgca ggcggaacac ggcccggcgc gtcgatcgat
      361 aggcgaaggc gagcgttagc aatgtgacga cggtgatgcc gatggcgagc gatagcacga
      421 tgcgcggcgc gtccgggttt ccgagcagga ccgccaccag aagggtatcg acgagggtcg
      481 tgatgttggc acccgccacg tacggcagga tctgctcacg acgcaccagg ccacgcgaga
      541 ccgccgggac cagcaggctc agcgcgaccg acaccgatag cgtggccagc gccaccagca
      601 ggccgatccc aaacaccgtc caggggccaa cgtaccagcg ggcgttaccg ccgctatccg
      661 cggcctccag cttcggcagc aaggcatcca ccagccggaa ggagacgagc agcagcagca
      721 cgccaagcgc gaagacggcc gctgctccaa gcgcgctctc tggcggcagg atgccctcga
      781 tggcgccgac cactggcgcc gtcgctgcat cggtcaggtc gaagaacacc ggcggtggcg
      841 tgaggctcac cgagtccagc gcgccggagc gcaggaggag cagtcccgcg atacccccgg
      901 ggacgtacat gagggcagtc atggccaggg ccagcacgcc aatggaaatg ggtgcacggc
      961 ggccgacggc gcgtccggtc agggcatacg cggcgcccac caccagcacc acgaaggcgg
     1021 cacccagcct cgagcccacc accatcccgt acgcctcgtc gaggtccagg aacccgccgt
     1081 cgaacagcgc caacgcggag gcggccaccg gcgatccgga cagcaccagc agcgagccga
     1141 gccaaccgat gccgagtgcg tccattgctc cccccaccgc ctcgccgagc gccggggcca
     1201 gccctcgcga tccgtccttc atgagtgcca gggccaccag gaacacccac agggcagcaa
     1261 cgcctatcgg caccctgcgg atgaggtcca gggttcgccg cgagcgcggg cgtaccgagg
     1321 tttgtgcttc ctcccactcg cggtgcagga cggcgaaggg tgcagggtcg aggtcgcccc
     1381 agatggcgat gcgctcgatc gccgccgcgc gcgcgtcgtc ctccggggca gcgtggtcgg
     1441 gcggatgcgg cggcgatggc acggcgcgca ataatagcgg ccctctcatt gaatgatggt
     1501 tgcctgtccg ggctgactgg agtcgaacgg ccgcaggcag cgtgaagcta ccgtaggagc
     1561 agtgacgaat gcagatcaca gtcgtgggcc tcggctatgt cggcctggtc acggccacct
     1621 gcctggcccg actcgggcag gacgtcatcg gcctggaggc cgatccggac aagctgcgtg
     1681 cgctggagcg ggacgaaccg ccgtttttcg agcccgggct ggagggtgag atcgcgttcc
     1741 agcgcagcac agggcgcctg cgcttcaccg gcgagtccgc ccaggcactg cgtacccccg
     1801 acgtcgtatt gatatgcgtg gggacgccgt cacgtccgag cggagaagcc gatctgcgcg
     1861 ccgtggagca ggtcatcgac agcatcgcgg gcgagctcca gaaccacgcc gtcatcgcgc
     1921 tgcgcagcac cgtcccagtg gggaccacgc gcaaggtcga ggcgcggctc aacgctcgcc
     1981 tcggcgagca cggcggcacc gcatcggtcc cggtctttgc caacccggag ttcctgcgaa
     2041 cgggacgtgc catagaggac ttcctgcggc cgtggcgcgt ggtgctgggc aagaccgagc
     2101 taccgaccga ggaggacgtc gagcggctgc tgacgctgta ccggcccctg gagagcccca
     2161 cgctggtctt cgatgctgag agcgccgagc tcgtcaagaa cgcggcgaac gcctacctgg
     2221 caactcgcat cagcttcatc aacgagctct ccgcgttgtg cgagcgcacg ggggcatcca
     2281 tcgatgcggt gatcagcggc atcgccagcg acccgcgcat cggcgcggac tacctgcgtc
     2341 cggggatcgg gtacggcggc tcgtgccttc ccaaggacgt ccgctcccta gtggccatgg
     2401 gcgaccacta caaggtgccg atgcagctgg ccctggccgt cgacgcaatc aaccagcagc
     2461 agccgctgcg cgccgcagag cgcttggccg acgagctggg tgggagcctg cagggacgtc
     2521 gcatcggact gctggggctc tccttcaagc cggacacgga cgacatccgc gactcgcccg
     2581 cgctggcgct ggcccgcgcg ctgaggggca tgggggccga tgtcgccgcc tgcgaccctg
     2641 aggcggccgc agccgcggcc gcgcgcgaac cttggatcga gatagtggcg ggaccatccg
     2701 aactggcggc gcgggcggac gcgctcgtgc tggccaccga gtggccggag tacgtcaccc
     2761 tcgacatggg gcccttggcg gctgacatgc gccagccgat cctgctggac gcccgcaacg
     2821 cgctcgacgc gaaggtggtg atggcggccg gcttccgtta cctgcgtgtc gggaacccag
     2881 ccggcgacgt taccgacctc ctggctgctc ggcccgaagc cagtcgacga agcgcctgat
     2941 cccgtcctcg aagcgggtgc ggggctgcca accgagcacc tcgcgggctc gggtgatgtc
     3001 ggcgtgggtg gccagcgcgt ctccgggctg gtgggggacg tcgcgccgga cggcgtccat
     3061 gccgagcgcc gtctcgagag cggtcagcat ttcgccgttt gtgatgggcc gatcgctccc
     3121 gacgttgacc accagcctgg gatggtctgt atccgccatg cggaccagcg cgtcgaccac
     3181 gtcgtcgacg taggtgtagt cgcgcgggac ggtccctgag ccgtagagct cgatcgggtc
     3241 accgcgcagg atgcgagtcg cgaaggtgtg gatggccagg tcgggacgtt ggcggggccc
     3301 gtacacggtg aacaggcgcg ccagcatcac cggcaagccc gtgagggcat ggtgggcatg
     3361 caccaacgcc tcgccggcca ccttcgtcgc gccgtagggc gagacggggc gggcggccat
     3421 ctggtcctcc ttgaacgggc ggcggtcgtc gttgccgtac accgagctgg acgaggcgaa
     3481 gacgagccgc gcgccgacac ctgcaaccgc cgccag
//
LOCUS       DATACA010000180         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_586453, whole genome shotgun sequence.
ACCESSION   DATACA010000180 DATACA010000000
VERSION     DATACA010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586453"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..851
                     /locus_tag="VHK28_03650"
     CDS             396..851
                     /locus_tag="VHK28_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2141362.1"
                     /translation="MIRVKLRLPASVHLTRASLDDVEALAEAFAGADAVAHLAGINRE
                     RGDATYEAVHVQGTRNVVAAAQRVGVPGSSCSVSCGPGRIADPATTSRSGRPRSSCEF
                     GRSKRPSPAYCARDVNSHRLRASAKPNPPIEPLRPRLIYSSPNAMTREQ"
     gene            988..1063
                     /locus_tag="VHK28_03655"
     tRNA            988..1063
                     /locus_tag="VHK28_03655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Ala,seq:ggc)
     gene            1157..1747
                     /locus_tag="VHK28_03660"
     CDS             1157..1747
                     /locus_tag="VHK28_03660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2141363.1"
                     /translation="MRTGTTNRIDSSGGTRGRARTTRDGRSWTLRAARPTDSRGLARL
                     FAEVRTEGRWLLTPPSAVSEPSEAFFIGEMIRNEGGLALVAEADGDVVGNVLVSVERS
                     TVSNHVGTLSIVISEAWRDVGIGGEMVRAAQDWVRERGLAKLALAVFPDNARAIAVYE
                     HAGFVREGLRRRQYRGEGGTFRDELLMAWFPEEPGS"
     gene            1744..>2221
                     /locus_tag="VHK28_03665"
     CDS             1744..>2221
                     /locus_tag="VHK28_03665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2141364.1"
                     /translation="MNAIDRDARARGFDAWADEYDRFRPGYPDELFGTVATQLGLPAH
                     PLVVDLGAGTGRAALAMAERGWRVTAVEPGRPMLDLLRAAASDAGLLVATVQAPAEAT
                     GLDPASADLVTAAQAFHWFDKDAALTEIARITKPGGGVALFWNMRDAARSPFLADYE"
BASE COUNT          340 a          740 c          805 g          336 t
ORIGIN      
        1 ccgccgagcc tccacaccgg ccgggctggc catggccgcc cgctcggtgt cgccgagggc
       61 gtcgaggctg gcgaactgct ctcgcaggcc tggaccaaac ccgtccagcg actctggcag
      121 gggcagctgt tgcatcttgc cgcggtagac cacgtggggg tagagcaccg cgaagaggtc
      181 ccgcttggtt cggaagtgac ggtagacggt agggactgat acaccggcct cgcgggcgac
      241 ggccggtatg gaggcacttg caagcccccg cgacatgacc cggacagtgg cgtccaggat
      301 ccggctgcgc gtctcctcgg cttgttgccc ccgcagcacg ctacggtagg ggcgtgatgg
      361 ctccgcattg gcgatatggt tcatattgtt aatatatgat ccgtgtcaaa cttcggttgc
      421 ccgcatcggt tcacctcacg cgcgcgtcgt tggacgatgt cgaggcgctg gcggaggcgt
      481 tcgccggagc ggatgccgtg gcgcacctgg ccggcatcaa ccgcgagcgc ggcgacgcaa
      541 cctacgaggc ggtccacgta cagggcacgc ggaacgtggt cgccgcggcg cagcgggtag
      601 gggtccccgg ctcgtcctgc tcagtttcct gcgggcccgg ccggattgcg gatccggcta
      661 ccacgagtcg aagtgggcgg ccgaggagct cctgtgaatt cggacgctcc aagcgcccat
      721 cgccggcgta ctgcgcacgt gatgtgaatt cccaccgctt gagggcgagc gccaagccca
      781 atccgccaat tgagccactc cgcccacggc tgatatactc gtccccgaac gcgatgacgc
      841 gggagcagta ggcgctgctc cgatcacgaa gcgagccggg acggtggaag ccggcgatcg
      901 agacgcgccg aactcgcccg ccgagcagcc tcgtcaaagc gaggccggag tggcgcccga
      961 tagaagcgcc gatgagtgga ccgacgtggg gttgtagctc agctgggaga gcacctgcat
     1021 ggcatgcagg gggtcggggg ttcgagtccc cccagctcca ccaatcttca tcggtcaagt
     1081 catggtggta ccgcgacggg agcccccttc gtcctgacga gagggggctt tgttgtaccc
     1141 gacggacggc agaccgatga ggaccggcac tacgaacagg atcgacagca gcggcgggac
     1201 tcgcggccgg gcgcgcacca cgcgcgatgg ccgcagctgg acgctgcgcg cggcgcgtcc
     1261 caccgactcc cgcggcctgg cccgcctgtt cgccgaggtg cgaaccgagg gccgctggct
     1321 gctgacgccg cccagtgcgg tgagtgagcc gtcggaggcg ttcttcatcg gcgagatgat
     1381 ccgcaacgag ggtggtctgg cgctggtcgc cgaggcggac ggcgacgtcg tcggcaacgt
     1441 gctggtcagc gtggagcgca gcaccgtgag caaccatgtc gggaccctct ccatcgtgat
     1501 aagcgaggcg tggcgcgacg ttgggatcgg gggcgagatg gtgcgcgccg cccaggactg
     1561 ggttcgcgag cgaggcctgg ccaagctggc gctggcagtt ttcccggaca acgcccgggc
     1621 catcgcggtg tacgagcatg ccgggttcgt gcgcgagggc ctgcgccgcc gccagtaccg
     1681 cggcgagggc ggcaccttcc gcgacgagct cctgatggcc tggttcccgg aggagcccgg
     1741 ctcgtgaacg ccatcgatcg cgacgcgcgt gcccggggct tcgacgcctg ggccgatgag
     1801 tacgatcgat tccgtcctgg ctacccggac gagctgttcg gcaccgtcgc cacccagctg
     1861 ggattgccgg cgcatccgct ggtggtggat ctcggcgcgg gcacgggccg ggccgcactg
     1921 gccatggccg agcgtggctg gcgggtcacc gcggtggaac ccggccgccc catgctggac
     1981 ctgctgcggg ccgcggccag cgatgccgga ctgctggtcg caaccgtcca ggcaccggcc
     2041 gaggccaccg ggctggatcc ggcgtccgcc gacctggtga ccgcggcaca ggccttccac
     2101 tggttcgaca aggacgcggc gttgaccgag atcgcgcgca tcaccaagcc cggcggcggc
     2161 gtggccctgt tctggaacat gcgcgacgcc gcacgctctc ccttcctggc cgactacgag
     2221 c
//
LOCUS       DATACA010000181         7170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_104858, whole genome shotgun sequence.
ACCESSION   DATACA010000181 DATACA010000000
VERSION     DATACA010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104858"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VHK28_03670"
     CDS             complement(<1..321)
                     /locus_tag="VHK28_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141365.1"
                     /translation="MDARIRDALGRQPGVDDWTIRREASRGVQLYLVGDRIENVRHVQ
                     HEAYELDLFNRHQTDAGGAVGSATLPVASSDLDRLGPLLTDGVTIAGSVHSPPWELPG
                     AGEPP"
     gene            complement(332..1771)
                     /locus_tag="VHK28_03675"
     CDS             complement(332..1771)
                     /locus_tag="VHK28_03675"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2141366.1"
                     /translation="MTAPNQDLSETDASAAARLRERFGFLREVVAELEREVPYASALV
                     RQADGIRIQLRDGDQQAGRQDPQAGVVLTVSNGAYLEEAATDRTDPDAVRQLARELAE
                     RARSHRPRAGEPALQLATNGVLEADFATPMQRPPNAESLVEKLERYESLRQRLRAVDS
                     RAVQAICTYGHNELSEVFVRRDGMVTQQLRRVVLALIVFVSDGQQRQYAYRGSGATGG
                     LEHIEVDDADLHDLAGVAVSMLSAKPVPPGTYDVVADPATAGTIAHEAFGHGVETDMF
                     VKGRARAADFIGRQVGSDLVNILDDPTLAGAYGSYFVDDEGQPAAPTTIIRNGVFERG
                     LTDLYSASRLGVARSANGRRQSVHRKAYARMSNTYFAPGTSSRQEVLESLDDGLLLCD
                     ALNGMEDPKGWGIQIWAHHAREYRGGRPTGIIYAPVAITGYVPDVLRDVTMVSDELAM
                     SPGTCGKGWKELVPVSSGGPYLRTRCRLG"
     gene            complement(1835..3130)
                     /locus_tag="VHK28_03680"
     CDS             complement(1835..3130)
                     /locus_tag="VHK28_03680"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141367.1"
                     /translation="MRRLVGFLRASTDALSERDFRLLWIGQSGSAVGDALVGVALAFA
                     VLELTSSAADLGLVLASFAVPRVVFTLAGGVWSDRLPRQRVMVACDLLRAAGQAVVSA
                     MLISGNAEIWHLVGLAFLMGTASAFFVPASVGLMPQLVSVERVQQANALVSLSQSAAH
                     IFGPLLAGLMVATVGAGWAFAVDGLSFLVSAGFLMAMRVPPRPPTERRPFVAELADGW
                     REVVSRSWVVAAILAFSLSNASLAAFQVLGPLVADRTLGGAAAWGVIVTGGSVGGLLG
                     GAISLRWKPRRPLVPGFVLMALATVQLFLLIPPFPAPVIAAGAMFTIGAIVVSNTFWD
                     TMLQQHIPRDAISRVSSYDWMVSLIFQPIALAAVGPMSEAIGIRETLLVAAAIGIAAN
                     GAVLLVPGVRRLERVDTSVAATPPEVAAAAGEATEPPLA"
     gene            complement(3173..3646)
                     /locus_tag="VHK28_03685"
     CDS             complement(3173..3646)
                     /locus_tag="VHK28_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141368.1"
                     /translation="MTDTARPARCTEPERRQFDFWIGDWDVLDPEGRTVGHNRITSLF
                     DGCAIREEWEGASGHRGTSLNAWSPETGWWSQTWVDSSGLVLRIEGGLRPEGMVMEGT
                     AVLPGSETSVPTRQRITWSPMDGDADRIRQHWEVSKDGSSWETVFDGHYRRRPAG"
     gene            3706..4179
                     /locus_tag="VHK28_03690"
     CDS             3706..4179
                     /locus_tag="VHK28_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141369.1"
                     /translation="MTDLEPGITLRPAAEADAPAVKRLVDDAYGHYQARIGRRPMPML
                     MDQAAAIRDREVWVLADDGRVVGVIDLQPDLDHVLIVNVAVDPQHQGRGLGSRLLVHA
                     EQRARELNLPEVRLYTNERFVENLALYGRRGYRETHRVPAGTTAVVHMRKRLDGG"
     gene            4189..4506
                     /locus_tag="VHK28_03695"
     CDS             4189..4506
                     /locus_tag="VHK28_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141370.1"
                     /translation="MAMTAEALRDLPARVISEATGANRRTAERWRAGTQPRRTVYRLR
                     LQDLATVWDLLGPGIRPRARLAWLTARSAHLGWQRPVDLLAAGEFERVRGAALAYSAG
                     DVT"
     gene            4584..5024
                     /locus_tag="VHK28_03700"
     CDS             4584..5024
                     /locus_tag="VHK28_03700"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEX2141371.1"
                     /translation="MPPEQPAPHAGRYHRVGDPWPLYAALDRETMWAEWAHATGGAVR
                     PEDEERWVCRLDVDLAVLDLRDAATRRALGVDERDLVGDWSPEAPNAATLRVAAVATE
                     LGVDGMVVPSAAHEGGWNLAVLPRAFDAVRLASRRRSRPSPPVS"
     gene            5070..5819
                     /locus_tag="VHK28_03705"
     CDS             5070..5819
                     /locus_tag="VHK28_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141372.1"
                     /translation="MKYGRGKGLAVVVTVMLLVAHAAPVAAEHAGRDIGSFSTCDRPV
                     NPPRCSSVGNDRWHFVYIDPSVPAALADSIRDAIAKYDATYLIVREQVAINHLTDAVA
                     FAADHGANGAAGWVYCPLDAPQGINHRGHRWCQRQEIHFNLNARYAVFFDDDASRDHL
                     ACHELGHTIGLRHWGNPPRTDGPAGATCLNADTPNGPLDLHQADVDHILDYYASPGAW
                     IAGALRDARPRRGDDAATGMQRLGGLLPRFD"
     gene            complement(6000..6686)
                     /gene="msrA"
                     /locus_tag="VHK28_03710"
     CDS             complement(6000..6686)
                     /gene="msrA"
                     /locus_tag="VHK28_03710"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX2141373.1"
                     /translation="MFFRNDHASRLPDPSEALPGRPDPIPTAERHFVNGQPLNGPYPE
                     GSEIADFALGCFWGEEKLFWETPGVIVTAVGYQGGTTPNPTYKEVCTGRTGHAESVRV
                     VYDPQQVSYDDLLRTFWEHHDPTQGFRQGNDVGTQYRSAIFTHTDAQAAAARASREMY
                     QAELSKAGYGEITTEIRPAPEFYFAEDYHQQYLAKVPKGYCPDHSTGVACPLDFSRLA
                     QVSQAEGASS"
     gene            complement(6755..>7170)
                     /locus_tag="VHK28_03715"
     CDS             complement(6755..>7170)
                     /locus_tag="VHK28_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2141374.1"
                     /translation="ADQLARARVQQIGLADDPHQRAIGHEAELLLKSYRISGLPVVTD
                     GALVGVISQTDLLNARSSELIGANWDRVRVRHLMTRPAVTVHMNTKALRSARLMLDQH
                     IHRLVVVDDEEVPIGVVTATDMLRLLLPEEAVAPV"
BASE COUNT         1068 a         2531 c         2550 g         1021 t
ORIGIN      
        1 cggaggctcg ccggcaccgg gcagctccca gggcgggcta tgcaccgacc cggcgatggt
       61 cacgccatcg gtcagcagtg gcccaaggcg atccaggtcg gagctggcca ccggcagggt
      121 ggcgctgccg acggcgccgc cggcatcggt ctggtgcctg ttgaacaggt cgagctcgta
      181 cgcctcgtgc tgcacgtggc gcacgttctc gatccggtcg cccaccaggt agagctgcac
      241 gccgcgactc gcctcgcgac ggatggtcca gtcgtccacc cccggctggc ggccgagggc
      301 atccctgatc cgggcgtcca cgcgctcctc ctcagcccag gcggcagcgg gtacggaggt
      361 acggaccgcc ggacgagacg gggacgagct ccttccagcc cttgccgcag gtcccggggg
      421 acatggccag ctcgtcggag accatggtca cgtcccgtag cacgtccggc acgtagccgg
      481 tgatggcaac cggcgcgtag atgatgcccg tgggccggcc gccgcggtac tcgcgggcgt
      541 ggtgcgccca gatctggatg ccccagccct tgggatcctc catgccgttc agggcgtcgc
      601 acagcagcag cccgtcgtcc aggctctcga gcacctcctg cctgctgctg gttcccggcg
      661 cgaagtacgt gttcgacatg cgcgcgtacg ccttgcggtg cacgctctgg cgccggccgt
      721 tcgcggagcg ggccacgcca aggcgactcg cggagtagag gtccgtcagc ccccgctcga
      781 agacgccgtt gcggatgatg gtggtgggag ctgccggctg gccctcgtcg tccacgaagt
      841 agctgccgta ggcgccggcc agggtcggat cgtccaggat gttgaccagg tcagaaccga
      901 cctggcgacc gatgaagtcc gcggcgcgcg cccggccctt tacgaacatg tcggtctcca
      961 cgccgtggcc gaaggcctcg tgggcaatcg tgccggccgt tgccggatca gcgacgacgt
     1021 cgtaggtccc cggcggcacc ggcttggcgc tcagcatcga gacggcgacg cccgccaggt
     1081 cgtgcaggtc ggcgtcgtcc acctcgatgt gctccaggcc gccggtggcc ccgctgccgc
     1141 ggtacgcgta ctgccgctgc tgcccgtccg agacgaagac gatcagggcc agcaccacgc
     1201 gccgcagctg ctgggtcacc atgccgtcgc gccgcacgaa gacctcggac agctcgttgt
     1261 gcccgtaggt gcagatggcc tgcaccgcac gcgaatcgac cgccctcagc cgctggcgca
     1321 ggctctcgta gcgctccagc ttctcgacca atgactcggc attcggcggc cgctgcatgg
     1381 gcgtagcgaa gtcggcctca agcaccccgt tggtcgccag ctgcagcgcc ggctcgcccg
     1441 ctctcggacg atggctgcgc gcgcgctccg cgagctcgcg cgccagctgc cgaaccgcat
     1501 cgggatcggt gcgatcggtg gccgcctcct ccaggtacgc gccgttcgat acggtcagga
     1561 cgacgcccgc ctgcgggtcc tgccggccgg cctgctggtc accgtcgcgc agctggatgc
     1621 ggatgccgtc cgcctggcgc accagggcgc tggcgtaggg aacctcccgc tcgagctccg
     1681 ccaccacctc tcgcaggaac ccgaaccgct cccgcagccg ggcagcggcc gaggcgtcgg
     1741 tttcggagag gtcctggttc ggggcagtca cgggcgtggc ggagggtagc aggcgaccgg
     1801 catcggcccg cgtcgccttt gcgacacccc cggctcaggc aaggggtggc tcggtggcct
     1861 cgccggccgc ggcggccacc tctggcggcg tggcggcgac cgaggtatcg acccgttcca
     1921 ggcggcgtac gccggggacc agcagcaccg cgccgttggc ggcgatgccg atggcggcgg
     1981 ccaccagcag cgtctctcgg atgccgatgg cctccgacat cggtccaacc gctgccaggg
     2041 cgatgggctg gaagatgagc gacaccatcc agtcgtagga gctcacccgg ctgatggcgt
     2101 cacgcgggat gtgctgctgc agcatggtgt cccagaaggt gttgctgacc acgatggcac
     2161 cgatggtgaa catcgccccg gcggcgatca ccggcgccgg gaagggcggg atcagcagga
     2221 agagctggac ggtggccagg gccatgagca cgaagcccgg caccagcggg cggcgcggct
     2281 tccagcgcag cgagatcgct ccacccagca ggccgcccac cgacccgccg gtgacgatca
     2341 cgccccaggc ggcggcaccg ccgagcgtgc ggtccgcgac gagcggcccc aggacctgga
     2401 aagcggccag cgaggcgttc gacagggaga acgccaggat ggcggccacc acccaggagc
     2461 gactcaccac ctcgcgccag ccgtcggcca gctcggccac gaacggccgc cgctcggtcg
     2521 gcggcctggg cggcacgcgc atggccatca ggaagccggc gctgaccagg aagctgagcc
     2581 cgtcgacggc gaacgcccag ccggcaccga cggtggccac catcagcccg gccagcaacg
     2641 gcccgaagat gtgcgccgcg ctctgcgaca ggctgaccag cgcgttcgcc tgctggacgc
     2701 gctccacgct cacgagctgc ggcatgagcc ccaccgaggc cggcacgaag aaggcggagg
     2761 cggtgcccat caggaaggcg agtccgacca ggtgccagat ttcggcgttg ccgctgatca
     2821 gcatggccga gacgaccgcc tggccggccg ctcggagcag gtcgcaggcc accatcaccc
     2881 gctggcgtgg caggcgatcg gaccagacgc cgccggccag ggtgaagacc acccggggca
     2941 cggcgaacga ggcgaggacg agcccgaggt cggcggccga gctggtcagc tccaggacgg
     3001 caaatgcgag cgccacgccc accagtgcgt cgccgaccgc ggagccgctc tgtccgatcc
     3061 agagcaggcg gaagtcacgc tccgagaggg cgtcggtcga ggcgcgcagg aaccccacca
     3121 accggcgcat gccggcagca gggtaacagg ggcgccttgc ggaaagtcgt cctcagccgg
     3181 cgggacggcg ccggtagtga ccgtcgaaca cggtctccca gctggacccg tccttcgaaa
     3241 cctcccagtg ctggcggatt cggtctgcat ccccgtccat cggtgaccag gtgatgcgct
     3301 gcctggttgg gaccgatgtc tccgacccgg gcaggactgc ggtcccctcc atgaccatgc
     3361 cctcgggccg caggcctccc tcgatgcgca ggaccagtcc cgaggagtcg acccaggtct
     3421 gggaccacca tccggtttcc ggcgaccagg cgttcaggct cgtcccgcgg tgcccgctgg
     3481 cgccctccca ctcctcgcgg atggcgcagc cgtcgaacag tgaggtgatg cggttgtgtc
     3541 cgaccgtgcg tccctccgga tccagcacgt cccagtcgcc gatccagaag tcgaactgcc
     3601 ggcgctctgg ctccgtgcag cgtgccgggc gggcggtatc ggtcatgcct ttccgtagcc
     3661 gcggagggcg cgaccggttc aatcgcgagc ggctaccttg accccatgac cgatctggaa
     3721 cccgggatca cgctgaggcc cgcggcggag gcggatgcgc cggccgtgaa gcggctggtc
     3781 gacgatgcct acggccacta ccaggcgcgc atcggtcgca ggccgatgcc gatgctgatg
     3841 gaccaggcgg cagccatcag ggaccgcgag gtatgggtgc tagcggacga cggccgggtg
     3901 gtgggtgtca tcgacctcca gccggacctc gaccacgtcc tgatcgtgaa cgtcgcagtg
     3961 gacccgcagc accagggccg ggggctgggg agccgcctcc tggtccacgc cgagcagcgg
     4021 gcacgggagc tcaaccttcc cgaggttcgg ctgtacacca acgagcggtt cgtggagaac
     4081 ctggcgctgt atgggcggcg gggctaccgc gagacccacc gggtgccggc cgggaccacg
     4141 gcagtggtcc acatgcggaa gcgcctggac ggggggtagc atcgggccat ggccatgacc
     4201 gccgaggcac tgcgcgacct gccggcccgc gtcatctccg aggctacagg cgccaaccgg
     4261 cgtaccgccg agcgctggcg agctggaacc cagccgagac gcacggtcta ccgactgcgc
     4321 ctccaggacc tggcgacggt gtgggacctg ctcggcccgg gcattcgccc ccgagcgcgg
     4381 ctggcctggc tcaccgcccg cagcgcgcac ctgggctggc agcggccggt cgacctgctg
     4441 gccgccggtg agttcgagcg cgtgcgcggt gcggcactgg cctactccgc cggggacgtg
     4501 acctgagccg gaggccgctc gcccccgtcg gccggcttcg ggcgcgggtc tggcgccaga
     4561 ccgcgatcca cgtaccgggt tgcctgccgc ccgaacagcc ggcgccgcat gccggccgct
     4621 accaccgggt cggcgatccc tggccgctgt acgcggccct ggaccgcgag acgatgtggg
     4681 ccgagtgggc gcacgccacc ggaggcgccg tccgtccgga ggacgaggag cgctgggtgt
     4741 gccggctgga cgtggacctg gccgtgctcg acctgcgcga tgccgccacc cgccgcgccc
     4801 tgggcgtcga tgagcgcgac ctcgtgggcg actggtcccc cgaagcgccg aacgccgcga
     4861 cgctgcgcgt ggcggcggtc gccacggagc tgggcgtgga cggcatggtg gtgcccagcg
     4921 ctgcgcatga ggggggctgg aacctggccg tcctgccgcg tgcgttcgac gcggtacgcc
     4981 tggcaagccg gcgccgctcc aggccgtcgc cgccagtttc ctgagcgtcc gggatggggg
     5041 agaatgcggg ctccacgggg agtgagcgcg tgaagtatgg gcgggggaaa gggctggcgg
     5101 tggtcgtcac ggtcatgctg ctggtggctc acgccgcgcc tgtggccgcc gagcacgccg
     5161 gccgggacat cggttccttc tcaacctgcg accggcccgt caatccgccc cgctgcagca
     5221 gcgtgggcaa cgaccggtgg cacttcgtgt acatcgatcc gtcagtcccg gccgcgctgg
     5281 cggattccat ccgcgacgcc atcgccaagt acgacgccac ctacctgatc gtccgcgagc
     5341 aggtggccat caaccacctg accgatgccg tggcattcgc cgccgaccac ggcgccaacg
     5401 gcgccgccgg ctgggtctac tgcccgctgg atgcgccgca gggcatcaac catcgggggc
     5461 atcggtggtg ccagcgccag gagatccact tcaacctgaa cgcccggtac gccgtcttct
     5521 tcgacgacga cgcttcccgg gatcacctgg cctgccacga gctgggccac accatcggcc
     5581 tgcgccattg gggcaacccg cccaggaccg atgggccagc cggcgccacc tgcctcaacg
     5641 ccgacacgcc gaacggcccg ctggacctgc accaggccga tgtcgaccac atcctcgatt
     5701 actacgcctc gccgggcgcc tggatcgcgg gcgctctgcg tgacgcaagg ccgcggcgcg
     5761 gcgacgacgc tgcgaccggt atgcagcgtc tcggaggttt gctgccgcgc ttcgactgag
     5821 gcggacgttc gcagccgcca aaagttgtca gcggctcctg tggagagccc cggatctgcg
     5881 atcgagctcg tttcgttgtc aacggctcgc cacgagagcc agtgacggct cccagcggcg
     5941 aacggcgatt cgcgggctcc gcagcggagc cagtgagcga tttctggctc gcaggcgagc
     6001 tagctgctcg cgccctccgc ctgtgaaacc tgcgccagtc gcgaaaagtc gagcgggcag
     6061 gcgacgccag tcgagtggtc cgggcagtag cctttcggga ccttggcgag gtactgctgg
     6121 tggtagtcct cggcgaagta gaactccgga gcaggtcgga tctcggtcgt gatctccccg
     6181 tagccggcct tcgagagttc ggcctgatac atctcacggg acgcgcgcgc ggccgcggcc
     6241 tgggcatcgg tgtgggtgaa gatggctgac cggtactgcg tgccgacgtc gttgccctgg
     6301 cggaagccct gggttggatc gtggtgctcc cagaaggtgc gcagcaggtc gtcgtaggag
     6361 acctgttgcg ggtcgtagac cacgcgcact gattcagcgt gcccggtgcg gccggtgcac
     6421 acctccttgt acgtcgggtt gggcgtggtt ccgccctggt agccgaccgc ggtgacgatc
     6481 acgcccggcg tctcccagaa cagcttctcc tcgccccaga agcagccgag ggcgaagtcg
     6541 gcgatctctg acccctccgg gtaggggccg ttcagtggct ggccgttgac gaagtggcgc
     6601 tcggctgtcg ggatgggatc cggacgcccg ggcagggcct ccgacgggtc cggcaaccgc
     6661 gaggcgtggt catttcggaa gaacatgctc acagtctagg cctggcgcat gacgatgccg
     6721 tgaagccagc gtgaaggtcc acccacgatc agagctacac gggcgccact gcctcctcgg
     6781 gcagcaggag ccgcagcatg tcggtggcgg tcaccacccc gatcggcacc tcttcgtcat
     6841 ccacgaccac cagccgatgg atgtgctggt cgagcatcag ccgcgccgag cgcagcgctt
     6901 tggtgttcat gtgcacggtg accgccggcc gcgtcatcag atgccgcacc cgtacgcggt
     6961 cccagttggc gccgatcagc tcgctcgagc gcgcgttcag cagatcggtc tggctgatga
     7021 cccccaccag cgcgccatcg gtcacgaccg gcaggccgct gatgcggtag ctcttgagga
     7081 gcagctccgc ctcgtgaccg atggcgcgct ggtgggggtc atcagccaga ccgatctgct
     7141 gaacgcgcgc tcgagcgagc tggtcggcgc
//
LOCUS       DATACA010000182         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1469889, whole genome shotgun sequence.
ACCESSION   DATACA010000182 DATACA010000000
VERSION     DATACA010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469889"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VHK28_03720"
     CDS             <1..1069
                     /locus_tag="VHK28_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141375.1"
                     /translation="VVSASASDPAPLQFLAPYAGVAMGEEFLDTGRDALCVYDDLSKH
                     AWAYRQVSLLMRRPPGREAYPGDIFYAHSRLLERAARLNEDNGGGSLTALPIIETQAG
                     DVSAYIPTNVISITDGQIFLQSDLFNQGQRPAVNAGISVSRVGGNAQIKAMRQVAGKL
                     RLDLAQYRELAAFAQFASDLDKATRDQLTRGEKLTLTLRQPQFSPLPVEKQVAIIWAA
                     TNGYLDDVESARVSEFETGFYRFLESSYEGLLPAIAKEKALSDDLTGQLTEAVKAFRD
                     QSGFGSEAGEASTGTDDAKTDDGAGTDEPAASGQAQAAPAQETAKAQGEEAGPSDAST
                     SGEGARIDEGAEGAPDKEGSR"
     gene            1073..1963
                     /gene="atpG"
                     /locus_tag="VHK28_03725"
     CDS             1073..1963
                     /gene="atpG"
                     /locus_tag="VHK28_03725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX2141376.1"
                     /translation="MASLKEIRARIGSVRNISQITRAMELVAASRMKRAQDAILAARP
                     YAEELHGALARISAAMDEQVHPLLARRTVRRIGLIVMTTDRGLAGALDSNTVRTALRY
                     VSERISQLEDGQRVEVSAITIGRKGRDQLRRAGIPIAAHFQRLGDRPSFSDVTPIARL
                     AAEDYLDGTYDEIDIVYPGFVTTLNQRPTVITLLPVGRPQDLSAEEAERTDEYLIEPT
                     PEAVLGRLLPHYVAVDLYRAVLEANASEQSARMVAMRSATDNANELIDDLTLVYNKTR
                     QATITREMIEIASGAEALGG"
     gene            1986..>2956
                     /gene="atpD"
                     /locus_tag="VHK28_03730"
     CDS             1986..>2956
                     /gene="atpD"
                     /locus_tag="VHK28_03730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2141377.1"
                     /translation="MATGKVIQITGPVIDVEFPPGELPAIYNALEIKRPPQARRAVGD
                     GDGAAPSEEILVVEVQQHLGNNWVRCVAMSTTDGLSRGLDAVDTGAAITVPVGEVTLG
                     RVFDVLGHPIDGKGTVKVKETLPIHREAPSFEEMETEAQVFETGIKVIDLIAPFKRGG
                     KVGVFGGAGVGKTVIIQELIRNIAQEHGGYSVFAGVGERSREGNDLIGEMTESGVIEK
                     TVMVFGQMNEPPGARQRVGLTGLTMAEYFRDVEGRDILLFIDNIFRFTQAGSEVSALL
                     GRMPSAVGYQPTLATEMGDLQERITSTTKGSITSLQAIFVPADDYTDP"
BASE COUNT          496 a         1032 c         1025 g          403 t
ORIGIN      
        1 cgtggtgtcg gcgtcggcct ccgatccggc gccgctccag ttcctggcgc cgtacgccgg
       61 cgtggcaatg ggcgaggagt tcctggacac cgggcgcgac gcgctgtgcg tgtacgacga
      121 cctctccaag cacgcctggg cgtaccgcca ggtgtcactg ctgatgcgcc gcccgccggg
      181 tcgcgaggca taccccgggg acatcttcta cgcccactcg cggctgctgg agcgcgcggc
      241 gcgcctgaac gaggacaacg gtgggggctc gctgaccgcg ctgcccatca tcgagacgca
      301 ggccggcgac gtgtccgcct acatcccgac caacgtgatc agcatcaccg acggccagat
      361 cttcctgcag tccgacctgt tcaaccaggg ccagcggccg gcggtcaacg ccggcatcag
      421 cgtgtcgcgg gtgggcggca acgcacagat caaggccatg cgccaggtgg ccggcaagct
      481 gcggctggac ctggcccagt accgcgagct ggcggcgttc gcccagttcg catcggacct
      541 ggacaaggcc acccgcgacc agctgacccg tggcgagaag ctgaccctca ccctgcgcca
      601 gccgcagttc tcgccgctgc cggtcgagaa gcaggtggcc atcatctggg ccgccaccaa
      661 cggctacctg gacgacgtgg agtcggcgcg ggtcagcgaa ttcgagaccg gcttctaccg
      721 cttcctggag tcgtcctacg agggcctgct gccggcgatc gccaaggaga aggcgctctc
      781 cgacgacctc accggccagc tgaccgaggc ggtcaaggcc ttccgcgacc agtccgggtt
      841 cggcagcgag gcaggcgagg cctctaccgg gaccgatgac gccaagaccg atgacggcgc
      901 cggcacagat gagcccgcag cctccggcca ggcgcaggcg gcgcccgcgc aggagaccgc
      961 caaggcccag ggcgaggagg ccgggccgtc cgatgcgtcc acctccggcg agggcgcgcg
     1021 catcgacgag ggtgccgagg gcgcccccga caaggagggc agccgctagc cgatggcaag
     1081 cctcaaggag atccgcgccc gaatcggctc ggtccgcaac atcagccaga tcacgcgggc
     1141 catggagctg gtggccgcct cgcgcatgaa gcgcgcgcag gacgccatcc tggccgcgcg
     1201 cccgtacgcc gaggagctgc acggggcgct ggcccgcatc agcgcggcca tggacgagca
     1261 ggttcacccg ctgctggcgc gccgcaccgt gcggcgcatc ggtctgatcg tgatgaccac
     1321 cgaccgcggg ctggccgggg cgctggactc caacacggtg cgcaccgcgc tgcgctacgt
     1381 cagcgagcgg atcagccagc tggaggacgg gcagcgggtg gaggtctcgg ccatcaccat
     1441 cggtcgcaag gggcgtgacc agctgcggcg cgcggggatc ccgatcgccg cccacttcca
     1501 gcggctgggc gaccggccgt cgttctcgga cgtgacgccc attgctcggc tggcggcgga
     1561 ggactacctg gacggcacct acgacgagat cgacatcgtg taccccggct tcgtcaccac
     1621 cctgaaccag cgcccgacgg tcatcaccct gctcccggtg gggcggccgc aggacctcag
     1681 cgcggaagag gcggagcgca ccgatgagta cctgatcgag cccacccccg aggccgtgct
     1741 cggccgtctg ctgccgcact acgtggcggt tgacctgtat cgcgctgtgc tggaggccaa
     1801 cgcgtccgag cagtcggcgc gcatggtcgc catgcgatcg gccaccgata acgcgaacga
     1861 gctcatcgac gacctgaccc tggtctacaa caagacgcgt caggccacca tcacccgcga
     1921 gatgatcgag atcgcctccg gggcggaagc cctcggcggc tagcgaagga gagacgtaca
     1981 cacttatggc caccggcaag gtgattcaga tcactggacc agtgatcgac gtggagttcc
     2041 cgcccgggga gctgccggcg atctacaacg cgctggagat caagcgtccg ccccaggcgc
     2101 ggcgtgcggt cggcgatggc gacggggcgg caccctcgga ggagatcctg gtggtcgagg
     2161 tgcagcagca cctgggcaac aactgggtcc gctgcgtggc catgtccacc accgatgggc
     2221 tgtcgcgcgg ccttgacgcg gtcgacaccg gggccgccat caccgtcccg gtgggcgagg
     2281 tgaccctggg tcgcgtcttc gacgtgctcg gccacccgat cgacggcaag gggacggtca
     2341 aggtgaagga gacgcttccc attcaccgcg aggccccgtc cttcgaggag atggagaccg
     2401 aggcccaggt cttcgagacc ggcatcaagg tcatcgacct gatcgcgccc ttcaagcgcg
     2461 gcggcaaggt cggcgtcttc ggcggcgccg gcgtgggcaa gacggtcatc atccaggagc
     2521 tgatccgcaa catcgcccag gagcacggtg gctactcggt cttcgcaggc gtgggcgagc
     2581 ggtcgcgcga ggggaacgac ctcatcgggg agatgaccga gtcgggcgtg atcgagaaga
     2641 ccgtcatggt gttcggccag atgaacgagc cgcccggcgc tcgccagcga gtcggcctga
     2701 ccggcctgac catggccgag tacttccgcg acgtcgaggg ccgcgacatc ctgctgttca
     2761 tcgacaacat cttccgcttc acccaggcgg gctcggaggt ctcggccctg ctgggccgga
     2821 tgccctccgc ggtgggctac cagccgacgc tggccaccga gatgggcgac ctgcaggagc
     2881 ggatcacctc gaccacaaag ggatcgatca cctccctgca ggccatcttc gtcccggccg
     2941 acgactacac cgatcc
//
LOCUS       DATACA010000183         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2112977, whole genome shotgun sequence.
ACCESSION   DATACA010000183 DATACA010000000
VERSION     DATACA010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112977"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VHK28_03735"
     CDS             complement(<1..243)
                     /locus_tag="VHK28_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2141378.1"
                     /translation="MTAARRRLPRMVPRMAGALVLLLAAGCASQVQPTPVATDVIDLP
                     QSYRFEPALITVPAGTTVTWTNNDQFTHNVRLLDDGG"
     gene            complement(240..551)
                     /locus_tag="VHK28_03740"
     CDS             complement(240..551)
                     /locus_tag="VHK28_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141379.1"
                     /translation="MTPIGDFVNAIPAPVFLLIHLTAFGIGAYFAWRSFEAGATLLGA
                     GFSLFALAEISYMTYHLDWTGVLFAHTISEVLDLSAFVLVFAAATRGAMAGLRGAPQQ
                     S"
     gene            complement(535..834)
                     /locus_tag="VHK28_03745"
     CDS             complement(535..834)
                     /locus_tag="VHK28_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141380.1"
                     /translation="MLVIQSVRRIWGELNRLPAIARLGLWIMSIAAAIDIVVHALGGG
                     QAAHGLPLAHGAHLLGITGMTLVWASVVLDGVRHQPTRRAIGSAKPGGPSRDAHR"
     gene            908..1606
                     /locus_tag="VHK28_03750"
     CDS             908..1606
                     /locus_tag="VHK28_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141381.1"
                     /translation="MTTRTREENLAAIGLLDEPVRLRVYDWVVGEGRPVGREETAQSL
                     GISRALATFHLDRLAEAGLLDTAYRRLTGRVGPGAGRPARVYWRAAGDVEVSLPDRRY
                     RLAADLFAAALRQIGGDRVPAELRQGARRLGEELGRKVASGRGSPGRRLMRALERGGY
                     EPIREKTGTIRLRNCPFDALVDEHRPLVCGTNLALSQGLASGAKSSGLEPVLDPQPGY
                     CCVAFVPKPAGASE"
BASE COUNT          255 a          552 c          630 g          236 t
ORIGIN      
        1 gcccccgtcg tccaggagcc ggacgttgtg cgtgaactgg tcgttgttgg tccaggtgac
       61 ggtcgtgccg gcggggaccg tgatcagcgc cggctcgaac cggtaggact gcggaaggtc
      121 gatcacgtcg gtggccaccg gggtcggttg cacctggctg gcacagcccg cagccagcag
      181 gaggacgagg gcgccggcca tacgcgggac cattcgcggc aggcgacgcc gggctgcggt
      241 catgactgct ggggcgcgcc gcggaggccc gccatcgcgc cgcgcgttgc ggcggcgaag
      301 acgagcacga atgcgctcag gtccagcacc tcgctgatcg tgtgcgcgaa cagcacgccg
      361 gtccagtcca ggtggtaggt catgtagctg atctcggcca gcgcgaacag cgagaagccc
      421 gcgcccagca gggtcgcgcc ggcctcgaag ctgcgccagg cgaagtaggc accgatgccg
      481 aaggcggtga ggtggatgag caggaagacc ggggccggaa tggcgttgac gaaatcaccg
      541 atgggcgtca cgagacggac ctccaggctt ggccgagccg atggcgcggc gggtgggctg
      601 gtgccgaacg ccgtcgagga cgacgctggc ccagacgagg gtcatgccgg tgatacccag
      661 caggtgcgct ccgtgcgcca ggggcagacc atgcgcggcc tgcccgccgc cgagggcgtg
      721 caccacgatg tcgatggcgg cggcgatgga cataatccac agccccagtc gggcgatggc
      781 cgggaggcga ttgagctccc cccagatgcg tctgacgctc tgtatcacta gcatcggttg
      841 gtgatacacc tgccgcaggc cggtgtcaag tgtatcgcca gcaactcggg gtgatagaat
      901 ccgtggcatg acgactcgca cccgcgagga gaacctggct gccatcggcc tgttggatga
      961 gccggtgagg ctgcgcgtct acgactgggt ggtcggcgag gggcgcccgg tcgggcgcga
     1021 ggagaccgcg caatcgctcg gcatcagccg tgcgctggcc accttccacc tcgatcggct
     1081 ggcggaggcc ggcctgcttg acaccgccta tcggcgcctg accgggcggg tgggacccgg
     1141 cgccgggcgg ccggcgcgcg tctactggcg cgccgcaggc gacgtggagg tttcactccc
     1201 cgaccgccgc taccgactgg ccgccgacct gttcgccgcc gcgctgcgcc agatcggcgg
     1261 cgatcgcgtt ccggccgagc tccgacaggg ggcccgaagg ctgggtgagg agctgggtcg
     1321 caaggtcgcc tcgggcaggg gttcgcccgg ccgccgcctg atgcgcgccc tggaacgcgg
     1381 cgggtatgag ccgatccggg agaagaccgg cacaattcgc ctgcgcaact gcccgttcga
     1441 cgccctggtg gacgagcacc ggccgctggt gtgcggcacc aacctggccc tgtcccaggg
     1501 attggccagt ggtgcaaagt cgagcgggct cgagcccgtc ctggatccgc agcccggcta
     1561 ctgctgtgtc gccttcgtcc ccaagccggc aggcgccagc gagtgagcgc cgacgtcgcc
     1621 gtcatcggcg gcgggatcat cggcacttcg gcagctgcca tgctcgccga gcg
//
LOCUS       DATACA010000184         7282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_105116, whole genome shotgun sequence.
ACCESSION   DATACA010000184 DATACA010000000
VERSION     DATACA010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105116"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2421)
                     /locus_tag="VHK28_03755"
     CDS             complement(<1..2421)
                     /locus_tag="VHK28_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX2141382.1"
                     /translation="MRTRLTNIALVLIGLGIPLLVAWLTRPRPAQVAARRESPPPTAA
                     RRTVVGPPMVEPPLLEPHPTGMFAALGRFAYRFRKPLPVAGLALAIGLTVWSFNAGGD
                     LIQGGWVISGSEEQEAAELLAQRFGEQASTLLVVYRDPDGDASDPAFQREVREGLEPI
                     EDDPIVDEILTWGETQAEDLLSTDGERTMAAVVLTEEVESAVDDAARLAERIRAPDGS
                     EMEVSGIPQLYHEFNDRIERDLVRAEAISLPIALVILLAVFGTLVAAGLPLLIAALSL
                     PTTFAVITLLATELEMSIFVTNIATMIGLALAIDYSLFMVSRFREELRHHSVEIAVER
                     MMGSVGKAVAVSGVAVAIGLSSLIVFEAAALRSMGIAGIVTVVSTLVYGLTVLPALLA
                     LLGPRVNRLRIPLPRALRLIEDDPSAADRRQGHGIWAWIAARVMRRPLLIAGPVLVLL
                     LLLGSPFASIQLSTGQNLEDLPPTPARLAFETILDEFPGGETDPITIAVVWPGEDLGE
                     SVSAERQAALAEYVDSIAELEGVTEIESALKPPPGLDAATYEQLLAQPAEQRPPEVRA
                     GLGEYLSEFVAEDTARVRVFSSLLPDSDAGRELVHDIRDLEAPDGARALTGGLPSRSA
                     DFMDSFWSSLPVALIIVVAVTGGVLFLTFGSVFLPVKAVLMSLISISASMGALVWIFQ
                     QGHLSDLLGFEPPGTTAAWLPIIMFAILFGLSMDYEVLLLSRIRERYVAHGDNTRAVA
                     EGIGLTGGIITGAALIMVAVFGAFGLSSIVFIKALGFTMALAVLIDATIVRGILVPAF
                     MRVMGRVNW"
     gene            2559..3914
                     /locus_tag="VHK28_03760"
     CDS             2559..3914
                     /locus_tag="VHK28_03760"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141383.1"
                     /translation="MTVLATPVAWWRGLGGLTRSGSSVEGLAWALYDFANTIFSFAIL
                     SFAMGPWTTMHLGETTGTFLFTLAASGAVALNAAVSPVLGAMSDRTGGRKRYLFAFTA
                     VCIVPTMFIGVLDIGLGLLAFAVANFGFQAALIYYDALLPDVARPETRGRLSGIGVSL
                     GYVGSIVSALLLGTTLDADGDITAASFVLVGSLFAIFAIPIFVLVRERPRPDAQRFSA
                     GAALRSWSQLASTVRRARQRPGLLRFVIGRFFYTDPVNTAIAVMSIFAINAVGFTAGE
                     ARYVLIGLIVVAIIAAFFWGILCDRIGPKRTLMLVLGSWAAGLLLIGLWLEKGPFLVA
                     GAILGSGLGGVAVTDRLYLLRLTRPEEVGEMLGLYGMAGKLSAVVGPLLFGAIVLVMD
                     PERNGNLAYQVAILSLLLLLVIGVLILRGVPEGTPHPQAEDRLGQPLEPSIVPPGETP
                     P"
     gene            3911..4666
                     /locus_tag="VHK28_03765"
     CDS             3911..4666
                     /locus_tag="VHK28_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX2141384.1"
                     /translation="MTLVVAHRGASAQLPENTMEAYRRAVEVGADAIELDVHLTADGQ
                     VAVIHDDILDRTTDATGPVAERTMDQLRSADAGARFQDAGGDFPFRGQGMTIPSLPEV
                     LDWLPDGLGLVVEIKARAAVGPTIELLRDSAVRRSGRLSVISFDEASIDQAHDLDPDL
                     PTGYLLVPTQPFEPALRWAVEHGHIGVHPWDGDLGLDPAPHLAQALAYGRLVGCYVVN
                     DPQRMQQLAAYGLWGFVTDVPDVARTALGPPER"
     gene            complement(4663..5064)
                     /locus_tag="VHK28_03770"
     CDS             complement(4663..5064)
                     /locus_tag="VHK28_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2141385.1"
                     /translation="MNAFIIELENRPGVLADITEAIAQKGINITAIAAATASDMGSVA
                     LLTNDEAGTRGALQGTGATFREVALVSSAIEDRPGSLAEAARRLAEAGVNIEALFATG
                     SEGGKVTIAFAVNDADAARTALGARTGAASA"
     gene            5172..5990
                     /locus_tag="VHK28_03775"
     CDS             5172..5990
                     /locus_tag="VHK28_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141386.1"
                     /translation="METPDHPPRLGFGLVARPDPDPSELGSLGARLEALGYTELWAND
                     GRGRAGLATLRAAAGSSRRLDLCLGVAPLSEVSPAQIAAEVARLELSPGRLVIGVGTG
                     SGSSLAMVRDGVAALRELLPGVRLAIAALGPRMCHLGGEVADVVLLNWALPERIAWSR
                     ERICEGAEASGRPMPRVASYVRVALGSDAPARLAAEAERYRARPRSYRRLFAEQAVAA
                     GEPPGIATADAAEVPALLAPYRAALDSCVVRALPAESSTEELLAIAAAAVMLPA"
     gene            5987..6895
                     /locus_tag="VHK28_03780"
     CDS             5987..6895
                     /locus_tag="VHK28_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX2141387.1"
                     /translation="MTYSIVARDPETGRFGIAVQSHYLGVGPVVPWLEAGIGAVATQA
                     SVNVSFGPIGLELLRAGRSSQEVVDALVASDDHPEIRQVGVVDAEGRAAAHTGAQCIP
                     AAGHHVGDGFTVQGNLLASDTCWPAMADAYQAGLREGAPFVERLLRAMEAAEGQGGDV
                     RGRQSAALMVHDGALHPAPWRGRLMDMRIEDHPDPVPELRRIVTLQLAYDLLDDSGDA
                     ARAGRSGEDRYAEARRLAPQAYELVFWRAIELANAGDLDAARREMAIAVGADQCWRDT
                     LQHLAEGRREGVTPELAAALLGEAGA"
     gene            complement(7133..>7282)
                     /locus_tag="VHK28_03785"
     CDS             complement(7133..>7282)
                     /locus_tag="VHK28_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2141388.1"
                     /translation="EGVFVAGDVHDHKYRQAVTAAGEGCKAAIDAERWLESVGEVEAA
                     TATNW"
BASE COUNT         1006 a         2602 c         2606 g         1068 t
ORIGIN      
        1 ccagttgacg cgccccatga cccgcatgaa ggcgggcacc aggatgccgc gcacgatggt
       61 ggcgtcgatc agcacggcca gcgccatggt gaagccgagg gccttgatga acacgatgct
      121 cgacaggccg aaggccccga acacggccac catgatcagc gccgccccgg tgatgatgcc
      181 gccggtcagg ccgatgccct ccgccacggc gcgcgtgttg tcgccgtgtg cgacgtagcg
      241 ctcacggatg cgggacagca gcagcacctc gtaatccatg gacaggccga agaggatggc
      301 gaacatgatg atcggcagcc aggcggcggt cgtcccgggc ggctcgaagc ccagcaggtc
      361 ggacaggtgg ccctgctgga agatccagac cagagcgccc atcgaggcgc tgatgctgat
      421 caggctcatg agcacggcct tgaccggcag gaagaccgat ccgaaggtca ggaacagcac
      481 cccgccggtg acggccacca cgatgatcag cgccaccggc agcgagctcc agaagctgtc
      541 catgaagtcg gccgatcggg acggcaggcc gccggtcagc gcgcgagcgc catcaggcgc
      601 ctccaggtcg cgaatatcgt ggacaagctc gcgaccggcg tccgagtccg gcagcaggct
      661 gctgaagacg cgtacgcgcg cggtgtcctc cgccacgaac tcggacaagt actcgcccag
      721 gccggcgcgc acctccggcg gtcgctgttc ggccggctgc gccagcagct gctcgtatgt
      781 cgccgcatcc aggccgggcg gcggcttcag ggcggactcg atctcggtca ccccctccag
      841 ctcggcgatt gagtccacgt actcggccaa cgcggcctgc cgctcggcgg acaccgactc
      901 ccccaggtcc tcgccgggcc agaccacggc gatggtgatg ggatcggtct cgccgccggg
      961 gaactcgtcc aggatggtct cgaacgccag gcgcgccggc gtggggggca ggtcctccag
     1021 gttctgcccg gtggacagct ggatggaggc gaacggcgag cccagcagca ggagcagcac
     1081 cagcaccggg ccggcgatga gcagcggacg gcgcatgacg cgcgccgcga tccaggccca
     1141 gatgccgtgc ccctgccggc ggtcggcggc ggacgggtcg tcctcgatca ggcgcagtgc
     1201 tcgcggcaac gggatgcgca gccggttgac gcgcgggccg agcagggcca gcagggcggg
     1261 cagcacggtg agcccgtaca ccagggtgga gaccacggtc acgatgccgg cgatccccat
     1321 ggatcgcagg gcggctgcct cgaagacgat gaggctcgaa agcccgatgg ccaccgccac
     1381 gccggagacc gccaccgcct tgcccacgct gcccatcatc cgctccacgg cgatctcgac
     1441 gctgtggtgc cgcagctcct cgcggaagcg gctgaccatg aacagcgagt agtcgatggc
     1501 cagcgccagc ccgatcatgg tcgcgatgtt ggtgacgaag atgctcatct ccagctcggt
     1561 tgcgagcagg gtgatgacgg cgaaggtggt cggcaggctg agggcggcga tcagcagcgg
     1621 caggccggcg gcaaccaggg tcccgaacac ggccagcagg atgaccagcg cgatgggcag
     1681 gctgatggcc tcggctcgca ccaggtcgcg ctcgatgcgg tcgttgaact cgtggtacag
     1741 ctgggggatc ccgctgacct ccatctcgga gccgtccggc gcccggatcc gctccgccag
     1801 gcgcgcagcg tcgtccacgg ccgactcgac ttcctcggtg aggaccacgg cggccatggt
     1861 ccgctcacca tcggtgctca gcaggtcctc ggcctgggtc tcgccccagg tcaggatctc
     1921 gtccacgatc gggtcgtcct cgatcggctc caggccctcg cgcacctcgc gctggaaggc
     1981 agggtcgctg gcgtcgccgt cggggtcgcg gtagaccacc agcagcgtgc tggcctgctc
     2041 gccgaagcgc tgggccagca gctccgcggc ctcctgctcc tccgagccgg agatcaccca
     2101 gccaccctgg atcaggtcgc cgccggcatt gaaggaccag acggtgagac cgatggccag
     2161 cgccaggccg gccaccggca gcggcttcct gaaccggtag gcgaagcggc ccagcgccgc
     2221 gaacatgccg gtcgggtgcg gctcgagcag cggtggttcg accatgggcg ggcctacgac
     2281 cgtcctgcgg gcggccgtgg ggggcggcga ctcgcgacgt gccgcgacct gcgccgggcg
     2341 cgggcgggtc agccacgcga ccagcagcgg tatgccgagg ccgatgagga ccagggcaat
     2401 gttggtgagc cgggtgcgca tgcctctcct gggtgcgatg agtccgtggt gtgcgggcag
     2461 aagggtaatg cgcatcggtt ccacgcgccg cgcgggccgt ggcgcgtgct gcgcgtcgct
     2521 atgatccgcg gcgtggatcg cgcgccgaga cccgccccat gaccgtcctg gcgacacccg
     2581 tcgcctggtg gcgcgggctg ggaggattga cccggtccgg cagctcggtc gagggcctgg
     2641 catgggccct ctacgacttc gccaacacca tcttcagctt cgccatcctc agcttcgcca
     2701 tggggccgtg gacgaccatg cacctcggcg agacgaccgg caccttcctg ttcaccctgg
     2761 ccgccagcgg ggcggtcgcc ctgaacgccg ccgtcagccc ggtcctgggc gccatgagcg
     2821 atcgcaccgg cggtcgcaag cgctacctgt tcgccttcac cgccgtctgc atcgtgccga
     2881 cgatgttcat cggcgtgctg gacatcggcc tcgggctgct ggccttcgca gtggcaaact
     2941 tcgggttcca ggccgcgctg atctactacg acgcgcttct gccggacgtg gcccggccgg
     3001 agacccgcgg ccgactgtcg gggatcggcg tgtctctggg ctacgtgggc agcatcgtgt
     3061 cggcactgct gctgggaacc acgctggacg cggacggcga catcacggcc gccagcttcg
     3121 tgctggtcgg ctcactgttc gccatcttcg ccatccccat cttcgtcctg gtacgggagc
     3181 ggccacgacc agacgcccag cgcttcagcg cgggggcggc tctgcgctcg tggtcgcagc
     3241 tggcgagcac cgtacggcgc gcccgccagc ggccgggact gctgcgcttc gtgatcggcc
     3301 gcttcttcta caccgacccg gtgaacaccg ccatcgcggt gatgagcatc ttcgccatca
     3361 acgcggtcgg cttcaccgcg ggtgaggcgc gctacgtgct catcgggctc atcgtggtgg
     3421 ccatcatcgc ggccttcttc tggggcatcc tctgcgaccg catcggtccc aagcgcacac
     3481 tgatgctcgt cctgggttcg tgggcggccg gactgctgct catcggtctg tggctggaga
     3541 aggggccgtt cctggtggcc ggcgccatcc tgggttccgg cctgggtggc gtggcggtca
     3601 ccgaccggct ctacctgctg cgcctcaccc gtccagagga ggtgggcgag atgcttggcc
     3661 tgtacggcat ggccggcaag ctttcggccg tggtggggcc gctgctgttc ggggccatcg
     3721 tgctggtcat ggacccggag cggaacggga acctcgcgta ccaggtggcc atcctgtccc
     3781 tgctgcttct gctggtgatc ggggtgctga tcctgcgcgg cgtgccggag ggaacgccac
     3841 atccgcaggc cgaggatcgc ctgggccagc cgctggagcc ctccatcgtg ccgcccgggg
     3901 agacgccgcc atgacgctgg tggtcgccca ccgcggcgca tcggcccagc tgccggagaa
     3961 caccatggag gcctaccggc gcgccgtcga ggtgggcgcc gacgccatcg agctggacgt
     4021 gcacctcacc gcggacggcc aggttgcggt catccacgac gacattctgg accgcaccac
     4081 ggatgcgacc ggtccggtgg ccgagcggac catggaccag ctgcggtcgg cggatgccgg
     4141 cgcccgattc caggacgccg gcggcgactt cccgttccgt ggccagggca tgaccatccc
     4201 cagcctgccc gaggtgctgg actggctgcc ggacgggctc ggcctggtgg tcgagatcaa
     4261 ggcccgggca gccgtggggc ccaccatcga gctgctgcgc gacagcgccg tgcggcgctc
     4321 cgggcgcctg tcggtgatca gcttcgacga ggcatccatc gaccaggcgc acgacctgga
     4381 tcccgacctg cccaccggct acctgctggt gcccacccag cccttcgagc cagccctgcg
     4441 ttgggcggtc gagcacggcc acatcggggt gcatccgtgg gatggcgacc tcggcctgga
     4501 cccggctccc cacctggccc aggcgctggc ctacggccgg ctggtgggct gctacgtggt
     4561 caacgacccg cagcgcatgc agcagctggc cgcctacggc ctgtggggct tcgtcaccga
     4621 cgtcccggac gtggcacgca ccgcgctcgg cccgccggag cgttaagcgg aggctgcccc
     4681 cgtccgggcc cccagcgcag ttcgcgccgc gtcggcatca ttcaccgcga aggcgatcgt
     4741 caccttgccg ccctcgctgc cggtcgcgaa cagggcctcg atgttcacgc ccgcctcggc
     4801 caaccgccgc gcggcctccg ccagcgaccc cggccggtcc tcgatggcgc tcgacaccag
     4861 ggccacctcg cggaacgtcg cgcccgtgcc ctgcaatgcg ccgcgagtgc cggcctcgtc
     4921 gttggtcagc agcgcgaccg agcccatgtc gctggcggtc gcggcggcga tggcggtgat
     4981 gttgatcccc ttctgggcga tcgcctcggt gatgtcggcc agcactccgg gccggttctc
     5041 gagctcgatg atgaacgcgt tcatgccgca cctcctccac ggcgccggcc gtggcccgcg
     5101 accgtccagc accgcggctg acccggccgg cagcaggcaa tccagtgtac gccgcctgtc
     5161 atcatggacc gatggagacg cccgaccatc cgccccgcct cgggttcgga ctggtcgcgc
     5221 ggccggaccc tgacccgtcc gagctcggct cgttgggcgc gcgcctcgag gcgctcggct
     5281 acaccgagct atgggccaac gacgggcgcg ggcgcgcggg gctggcgacc ctccgggcgg
     5341 cggcgggcag cagccgacgg ctcgacctgt gcctgggggt tgcgcctctc tcggaagtgt
     5401 cgccagcgca gatcgccgcc gaggtcgctc gcctcgagct gtcgcccgga cggctggtga
     5461 tcggcgtggg caccggctct ggctcatcgc tggccatggt gcgcgacggc gtcgccgccc
     5521 tgcgcgagct gctgcccggc gtgcgcctgg cgatcgccgc gctcggcccg aggatgtgcc
     5581 acctcggcgg cgaggtggct gacgtggtgt tgctgaactg ggcgctgccg gaacggatcg
     5641 cgtggtcgcg cgaacggatc tgcgagggag ccgaggcgtc cgggcgaccg atgccgcgcg
     5701 tggcgtccta cgtccgcgtg gccctcggct ccgacgcgcc cgccaggctg gcggccgagg
     5761 cggaacgcta ccgcgcgcgg ccgcgctcct acagacgact gttcgccgag caggcggtag
     5821 cggccggcga gccgccgggc atcgcgaccg cggacgctgc cgaggtgccg gccctgctcg
     5881 cgccctaccg cgcggccctg gacagctgcg tggtccgcgc cctgcccgcc gaaagctcca
     5941 cggaggagct gctggccatc gcggctgccg ccgttatgct cccggcatga cgtacagcat
     6001 cgtggcgcgc gacccggaga ccggacgctt cggaatcgcc gtgcagtcgc actacctggg
     6061 ggtggggccg gtcgttccat ggctggaggc ggggatcggc gcagtcgcca cccaggcatc
     6121 ggtgaacgtc tcgttcggcc ctatcggtct cgagctgctg cgcgccggac gatcctcgca
     6181 agaggtggtt gacgcgctgg tcgcctccga tgaccatccc gagatccggc aggtcggcgt
     6241 ggtggacgcg gagggccggg cagccgcaca cacgggcgcg caatgcatcc cggccgccgg
     6301 acatcacgtc ggcgacggct tcaccgtgca gggcaacctg ctggcatccg atacgtgctg
     6361 gccggccatg gcggacgcct accaggcagg actccgcgag ggagcgccgt tcgtcgagcg
     6421 cctgctgcgt gccatggagg ccgccgaggg ccagggcggc gacgtccgcg ggcgtcagtc
     6481 agcggcgctc atggtccacg acggtgcgct gcacccagcg ccgtggcgcg gccggctgat
     6541 ggacatgcgc atcgaggatc atcccgaccc ggttcccgag ctgcggcgca tcgtgaccct
     6601 ccagctggct tacgacctcc ttgacgacag cggggacgcc gcccgagccg ggcgcagcgg
     6661 ggaggatcgc tacgcggagg cccgccgcct ggcgccgcag gcgtacgagc tcgtcttctg
     6721 gcgggcgatc gagctggcca atgccgggga ccttgacgct gcgcgccggg agatggcgat
     6781 cgccgtgggt gctgaccaat gctggcggga caccctgcag cacctggccg agggccggcg
     6841 cgagggcgtc accccggagc tggctgcggc cctgctcggc gaggccgggg cgtagccggg
     6901 gctcggtagg cagcactggc ctggccactt ctccacgatg taccggggga ccgccagttt
     6961 cgtcccgagt tgtcgtaccg gtccgacctg accgcgacga tcccggcccg ccgggcccct
     7021 ttcgtcgccg gtgcgccttg cgggtctcca ttccccaacg aagtggcggt gcggacggca
     7081 gttcctctgc gagctgccgc accagtaccg caatgacgtc cgggcgcggg ccttaccagt
     7141 tggtggcggt cgccgcctcc acctcgccca ccgactccag ccagcgctcg gcgtcgatgg
     7201 ccgccttgca gccctcaccg gcggcggtga ccgcctggcg gtacttgtgg tcgtggacgt
     7261 cgcctgccac gaacacgccc tc
//
LOCUS       DATACA010000185         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_105135, whole genome shotgun sequence.
ACCESSION   DATACA010000185 DATACA010000000
VERSION     DATACA010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105135"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1680)
                     /gene="aspS"
                     /locus_tag="VHK28_03790"
     CDS             complement(<1..>1680)
                     /gene="aspS"
                     /locus_tag="VHK28_03790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX2141389.1"
                     /translation="GWVHRRRDHGHLTFLDLRDRYGITQVVTSAEDAPDAHAAADPVR
                     NEWVIHVEGVVRHRPEGTANAELPTGAIEVGVDRLAVLNPSKVPPFYVNEEQPGLDES
                     LRLKHRYLDLRRPPLQQRILLRSRLASAIRRALEDEGFVEIETPTLIHSTPEGARDFV
                     VPSRLRPGEFYALPQSPQQLKQLLMVAGYDRYFQLAHAYRDEDLRGDRQPEHTQVDIE
                     MSFVREEDVMATVEAMVTRVTREVLPERPILASPFPRLSYEEAIARFGSDKPDLRFGI
                     ELVDLTDALRGLDFRVFAEPIAAGGAVYAIRAPGCGDYSRKQLDELTDLAQRHGARGL
                     AHLAVEMDGSLRGPAAKFLGTAEDEVRSLTQAGPGDLLLIVADAHRVHAAEALGHVRL
                     EMGERLGMRDPEVLAFVWVHRFPMFAWDEEGNRWDATHNPFSAPVPEEAERMEADPAS
                     VHAQQYDLALNGWELGGGSVRIHERPMLERAFALMGHTVEGMRDQFGALLDALEYGAP
                     PHGGIAIGLDRWAALFAGVDNIREVMSFPKTQSGTDPMMGAPSPISPQQLD"
BASE COUNT          236 a          580 c          611 g          253 t
ORIGIN      
        1 tcgtcgagct gctgcgggct gatgggcgag ggcgcgccca tcatcggatc ggtccccgac
       61 tgggtcttgg ggaaggacat cacctcgcgg atgttgtcga ccccggcgaa cagggcagcc
      121 caccggtcca ggccgatggc gatgccgccg tgcggcggcg ccccgtactc cagggcgtcc
      181 agcagggcgc cgaactggtc gcgcatgccc tccacggtgt ggcccatcaa cgcgaaggcg
      241 cgctccagca tcggtcgttc gtgaatgcgg accgatcctc cgcccagttc ccagccgttg
      301 agggccaggt cgtactgctg cgcatggacc gatgccggat cggcctccat gcgctccgcc
      361 tcctccggca ccggggcgct gaaggggttg tgggtggcgt cccagcggtt cccctcctcg
      421 tcccaggcga acatcgggaa gcggtggacc cagacgaagg caagcacctc ggggtctcgc
      481 atgccgagcc gctcgcccat ctccaggcgg acgtggccca gcgcctcggc ggcgtgcacc
      541 cggtgggcgt cggccacgat cagcaggagg tccccgggtc cggcctgggt cagggaccgc
      601 acctcatcct cggcggtacc caggaacttg gccgccggac cgcgcagtga gccgtccatc
      661 tccaccgcca ggtgggccag tcccctggcg ccgtggcgct gtgcgaggtc ggtgagctcg
      721 tcgagctgct tgcgcgagta gtcgccgcag cccggcgcgc ggatggcgta caccgcgcca
      781 ccggcggcga tcggctcggc gaagacacga aagtcgaggc cgcgcagggc atcagtcagg
      841 tccaccagct ctatgccgaa gcgcaggtcg ggcttgtccg agccgaatcg agcgatcgcc
      901 tcctcgtagc tgaggcgcgg gaaggggctg gccaggatcg gccgctctgg gagcacctcg
      961 cgcgtcacgc gggtgaccat ggcttccacc gtggccatca cgtcctcctc gcggacgaag
     1021 ctcatctcga tgtccacctg ggtgtgctcc ggctgccgat cgccgcgcag gtcctcgtcg
     1081 cggtaggcat gggccagctg gaagtagcgg tcgtagccgg ccaccatcag cagctgcttc
     1141 agctgctgcg gcgactgcgg cagcgcgtag aactcgcccg gccgcagccg tgagggaacc
     1201 acgaagtcgc gggcgccttc cggcgtggag tggatgaggg tgggggtctc gatctccacg
     1261 aagccctcgt cctccagcgc tcgccggatg gcggacgcca ggcgcgagcg aagcaggatc
     1321 cgctgctgca gcggagggcg gcgcagatcg aggtaccgat gcttcagccg cagcgactcg
     1381 tccaggccgg gctgctcctc gttgacgtag aagggcggca ccttgctggg gttgaggacg
     1441 gcgaggcggt ccaccccaac ctcgatggcg ccggtcggta gctcggcgtt cgccgtgccc
     1501 tccgggcggt ggcgtaccac gccctcgacg tggatcaccc actcgttgcg caccgggtcg
     1561 gcggcggcgt gggcgtccgg ggcgtcctcg gcggaggtca ccacctgggt gatcccgtac
     1621 cggtcccgca ggtcgaggaa ggtgaggtgt ccgtggtcgc gccgacggtg gacccagccg
//
LOCUS       DATACA010000186         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_186276, whole genome shotgun sequence.
ACCESSION   DATACA010000186 DATACA010000000
VERSION     DATACA010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186276"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VHK28_03795"
     CDS             <1..495
                     /locus_tag="VHK28_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141390.1"
                     /translation="HPPPPAAVGPTGRVGRDTRLAAVVLAALGAVLLIAGVLGLAAGL
                     YLSGWIHDRLPEITADSAAVGGAAFALGASLVGLAIVHLGLSLAVARTVRAMLLPAAV
                     LSLGMTVAVAGWSVAALVSAAAGVVDPPPMLAAASLLAVVAAAYALAARSVIRAWRST
                     RTRT"
     gene            542..618
                     /locus_tag="VHK28_03800"
     tRNA            542..618
                     /locus_tag="VHK28_03800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:576..578,aa:Asp,seq:gtc)
     gene            complement(663..1115)
                     /locus_tag="VHK28_03805"
     CDS             complement(663..1115)
                     /locus_tag="VHK28_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2141391.1"
                     /translation="MIKEEGNVIIRRPLEEVFAYVSDLTHSAEWQGGLLEVRKLTEGP
                     IGVGTRYAFVRQFLGRQMESTNEFVAYEPDVTVRFKIPSGPMPGQGTYLFEATPDGTK
                     VTARVELEPIGLTRLAGPLIAASLRREIATGLPVLKELLERQVAWDPA"
     gene            1218..1910
                     /locus_tag="VHK28_03810"
     CDS             1218..1910
                     /locus_tag="VHK28_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141392.1"
                     /translation="MSGVKRLESATASRRERAQATQRRIVEAAYRLFCERGYAASTFK
                     QIAQTAGVAVQTVYFMFNTKSTLLSRAYDYAVMGHEEPLVPADQRWYRQMVAAADVVA
                     AIRHLVAGVGEITRRVTPLYLVARATASGDPEVARVVDFHERWRADGYREMLEILRAK
                     ASLREGLSPERANDLLLLYAGMDAYHLLVDVRGWAYDEWVDWTVSVVADQVFGRHADA
                     SALQRSPAECAG"
BASE COUNT          278 a          680 c          656 g          369 t
ORIGIN      
        1 catcctcctc cgcccgcggc cgtaggcccc accggccgcg tgggccgaga cacccgcctg
       61 gcggctgtgg tactggcggc cctgggtgcc gtcctgctga tcgccggcgt gctggggctg
      121 gccgccggcc tgtacctgtc ggggtggatc cacgaccgcc tgccggagat cacggctgac
      181 tcggcggcgg tgggcggcgc cgccttcgcg ctgggcgcgt cgctggtcgg gctggcgatc
      241 gtgcaccttg gcctgtcgct ggccgtcgcc cggacggtgc gagccatgct gctcccggcg
      301 gcggtactca gcctgggcat gaccgtggcc gtggccgggt ggtcggtggc cgcgctggtg
      361 agcgccgctg cgggtgtcgt cgacccgcct ccgatgcttg ccgccgcgag cctcctggcc
      421 gtggtggcgg ccgcctacgc gttggcggct cgcagcgtca ttcgggcctg gaggagcacc
      481 aggacccgga cctgaaggtc ctcccgatcc ttgttatact cgcgcccgcc gtcactcccg
      541 cgggcccgtg gtgtagtggc ccaacatgcc agcctgtcac gctggagatc gccggttcga
      601 atccggtcgg gcccgccaat gctcgtacct cgtcaccccg gccgctcggt cgtcggattg
      661 agtcacgcag ggtcccacgc cacctggcgc tccagcaatt ccttgagcac cgggaggcct
      721 gtcgcgatct cgcgcctcag gctggcggcg atgaggggcc cggccagtcg tgtcaggcct
      781 attggctcca gctcgacgcg cgcggtgacc ttggttccgt ccggtgtcgc ctcaaacaag
      841 tatgtgcctt ggccgggcat gggacctgac gggatcttga accgtaccgt cacgtccggt
      901 tcgtaggcca cgaactcgtt ggtcgactcc atctgccgcc ctaaaaactg gcgaacgaag
      961 gcataccggg tgccgacgcc gattgggcct tcggtcagct tccgcacctc caagagcccc
     1021 ccttgccact ctgccgaatg cgtgaggtcg ctcacgtagg cgaacacttc ctccagaggg
     1081 cggcggatga tgacattgcc ttcctctttg atcatcccgc catccctcgt ttaaagcgcc
     1141 ggcatccata cgtcagaact ggagcgttac actagagcga cacactagcg ctcagcgctg
     1201 gtattctgct cgacgccatg tctggtgtca agcgtttgga gtcggccact gcgtcacggc
     1261 gtgagcgtgc ccaagcgact cagcgccgga tcgtcgaggc cgcctaccgg ctcttttgtg
     1321 aacgggggta cgcagcatcg accttcaagc agattgccca gaccgccggc gtcgccgtcc
     1381 agacggtgta cttcatgttc aacacgaagt cgaccctgct cagtcgtgcc tacgactacg
     1441 cagtgatggg ccacgaggag ccgctcgtgc cggccgacca gcggtggtat cggcaaatgg
     1501 tggccgcggc ggacgtcgtc gcagcaattc gccacctcgt cgccggtgtg ggcgagatca
     1561 cgcgccgcgt gacgccgctg tacctggtgg cgcgggccac ggcgtcaggc gatcccgaag
     1621 ttgcccgcgt cgtcgacttc cacgagcgct ggcgggcgga tggctaccgc gagatgctgg
     1681 agatcctgcg cgccaaggct tcccttcgtg aagggcttag cccggaacgt gcgaacgacc
     1741 tgttgctgct ctacgccggt atggacgcct accacctgct ggtcgacgtc cgtggctggg
     1801 cttatgacga atgggtcgat tggaccgtct cggtcgtggc tgaccaggtc tttggccgcc
     1861 atgctgatgc cagcgcgctt cagcgatccc cggccgaatg tgcaggctag ccagcgacac
     1921 tcacgccgaa ccggggagcc cctatggagc ccttaatcga ccgcgtctca tgcgtctcct
     1981 gcg
//
LOCUS       DATACA010000187         2715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_105291, whole genome shotgun sequence.
ACCESSION   DATACA010000187 DATACA010000000
VERSION     DATACA010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105291"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VHK28_03815"
     CDS             <1..569
                     /locus_tag="VHK28_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141393.1"
                     /translation="RATGVERQQLKWFLYGGALLVVAFVLEPLAAAVPALRPAADLAF
                     PLALAAMPVAIALAILRYGLYEVDRIISRTIGYALVSATLLAVYAGGALVLSQLTVPF
                     TGDQTVAVAASTLAVVALFRPARRRIQAAVDRRFYRSRYDAQRTLDEFMGLVRDEVDV
                     ERLSAQLRSAATSTLHPAAVSVWLRDRG"
     gene            574..1731
                     /locus_tag="VHK28_03820"
     CDS             574..1731
                     /locus_tag="VHK28_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141394.1"
                     /translation="MNRRLVLSSAAGVAVLAMLLAMALWLFGVDEPFTPEIVLYPTTY
                     LSFAVVGAAILARRPGNIIGGLCLAIGLVGSLVAVIDSYARLTAQPPGQDWAAWITAW
                     AFPSTLGPILLVLLLFPNGAPQSARWRPVVWLVVVGSLVVVAGAALTPTLADFPNVDN
                     PVGQGWVNDTPLAGGGVGWFALIIGALAAAVGLGVRLRRSAGIERQQLKVVAFAAALN
                     GISWVALALDLPGAAGELAVYAVFATFAAVPIAIGVAMLRYRLYDFDLIVRRTLVYGA
                     LTVLLGGVYVGLVLGLPLVLDPFTPDDSLAVAISTLGVAALFGPARSRVQAAVDRRFY
                     RARYDARRTLEQFAGQVRDQVDIDLLVGELNRVARDTVHPKSLSIWLRQQP"
     gene            1796..2560
                     /locus_tag="VHK28_03825"
     CDS             1796..2560
                     /locus_tag="VHK28_03825"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HEX2141395.1"
                     /translation="MSLDSHTPTSTPRRSDADAWASPVDRLRVDPTSGGVAGTVSGRR
                     LAGPLQGFGQMWQKTFRVRLDGAGPEVTPESVIAAWKEHFPEFWPRGNTFYAPLAGIR
                     PGEVALLAVRATGPVRLHTGVMVMYADDTSFTLMTPEGHMLTGWITFSAFRAKDGVVV
                     AQAQALERTSDPLFELGYALRFGHRANNRFWEQTMEAVARFQGVETRAETTVVCVDRR
                     RQWRHAGNVRHSAAFRSALHGVSAPLRWVRRAGARD"
BASE COUNT          339 a          938 c          948 g          490 t
ORIGIN      
        1 gccgcgccac cggcgtggag cgtcagcagc tgaagtggtt cctgtacggc ggcgccctgc
       61 tggtggtcgc cttcgtcctc gagcctctgg ccgccgcggt ccccgccttg cggccggcgg
      121 ccgacctggc cttcccgctg gcgctggccg ccatgccggt ggccatcgcc ctggccatcc
      181 tgcgctacgg cctgtacgag gtcgatcgaa tcatcagccg caccattggc tacgcgctgg
      241 tcagcgcgac gctgctggct gtgtatgccg gaggcgcgct ggtgctctcc cagctgaccg
      301 tgcctttcac gggggaccag acggtggccg ttgcggcatc gacactggcc gtggtcgccc
      361 tcttccgccc ggcacgccgt cgcatccagg cggccgtcga ccgccgcttc tatcggtctc
      421 gctacgacgc gcagcgcacg ctggacgagt tcatgggcct ggtgcgtgac gaggtggacg
      481 tcgagcgtct ttcggcccag ctccgatccg ctgcgacatc gaccctgcac ccggcggccg
      541 tctccgtctg gttgcgcgac cgcgggtaga ccgatgaacc ggaggcttgt gctgtcctcg
      601 gccgcgggtg tggccgtgct ggccatgctc ctcgccatgg ccctgtggct gttcggcgtc
      661 gacgagccgt tcacgcccga gatcgtcctc taccccacca cctatctctc gttcgcagtc
      721 gtcggcgcgg cgatcctggc cagacgcccg ggcaacatca tcggtggcct gtgcctggcc
      781 attggcctgg tcggctcgct ggtggccgtc attgacagct acgcacgcct gacggctcaa
      841 ccgccggggc aggactgggc ggcctggatc acggcgtggg ccttcccctc gacgctcggc
      901 cccatcctcc tggtcctgct gctgtttccc aacggggcgc cgcagtcggc ccgatggcgc
      961 ccggtggtct ggctggtggt cgtgggctcg ctggtggtcg tggccggtgc cgccctgacc
     1021 ccgacgttgg cggacttccc caacgtcgac aatccggtcg gacaagggtg ggtcaacgac
     1081 accccactcg cgggaggagg cgtcggatgg ttcgcgctga tcatcggtgc gctggcggcc
     1141 gccgtggggc tgggcgtcag gctgcggcgt tcggctggca tcgagcgcca gcagctcaag
     1201 gtcgtggcct ttgctgcggc gctgaacggc atctcctggg tcgccctggc actggaccta
     1261 ccgggcgccg ccggcgaact cgcggtttat gccgtgttcg ccacgttcgc cgcggtcccc
     1321 atcgccatcg gggtggccat gctccgctac cggctctacg acttcgatct catcgtccgc
     1381 cggaccctgg tctatggcgc gctcacggtg ctgctgggcg gcgtgtacgt cggcctggtg
     1441 ctgggccttc cgctggtgct cgaccccttc acccctgacg acagcctggc ggtggctatc
     1501 tcgacgctgg gagtcgccgc cctcttcggg ccggctcgct cacgggtcca ggcggccgtg
     1561 gacaggcgct tctaccgcgc acggtacgac gctcgccgca cgctcgagca attcgccggg
     1621 caggtgcgcg accaggtcga catcgacctc ctggtgggcg agctaaaccg ggtggcgcgt
     1681 gacacggtgc atcccaagag cctctcgatc tggctgcggc agcagccgta acgattctcg
     1741 gacggtcgcg cgggagtatc ggtccagcct gaacttcctc gctggagctg gactcatgag
     1801 cctggattcg cacaccccaa cctcaacccc gcgtcgctct gacgccgacg cctgggcgtc
     1861 accggtcgat cgcctgcgcg tggaccccac gtcgggcggc gtggccggca cggtgtccgg
     1921 gcggcgcctg gcgggcccgc tccagggctt cggccagatg tggcagaaga cctttcgggt
     1981 tcggctggac ggcgccggtc cggaggtgac gccggagagc gtgatcgccg cctggaagga
     2041 gcactttccg gagttctggc cgcgcggcaa cacgttctat gcgccgctgg ccggcattcg
     2101 gcccggcgag gtggcgcttc tggctgtacg tgccactggc cccgttcgcc tgcacaccgg
     2161 cgtgatggtg atgtatgcag acgacacctc cttcacgttg atgacgccag agggacacat
     2221 gctgaccggc tggatcacct tctccgcatt tcgcgcgaag gacggggtgg tggtcgccca
     2281 ggcacaggcg ctggagcgca ccagtgaccc gctattcgag ctgggctatg cgctgcgctt
     2341 cgggcaccgg gccaacaacc ggttctggga gcagaccatg gaggcggtgg cgcgtttcca
     2401 aggcgtcgag acgcgggctg agacgacggt ggtttgcgtg gaccgtcgac gccagtggcg
     2461 ccacgccggc aacgtgcggc actctgcggc cttccgctca gcgctccacg gtgtcagtgc
     2521 cccgctgcga tgggtgcggc gcgccggtgc ccgtgattga ggaggagggc cgaccaagcg
     2581 gatcgaggat gagttggtgg agatggggga gaatcgaact ccccgtccag aaccgagtcc
     2641 ccggaaacgt actacaggta tatccggcct acttggcgtt tcgctggcgg gcccccgacc
     2701 ggcggggtgc tccgt
//
LOCUS       DATACA010000188         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_426604, whole genome shotgun sequence.
ACCESSION   DATACA010000188 DATACA010000000
VERSION     DATACA010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426604"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /gene="recJ"
                     /locus_tag="VHK28_03830"
     CDS             complement(<1..1736)
                     /gene="recJ"
                     /locus_tag="VHK28_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX2141396.1"
                     /translation="MIRPRLEWLLPEPLFDPPAFAGFGPPVATLLARRGFRTQPELDT
                     FLGAGAQSLHPVELMADAGVALDRLETAVDRGERIAIWGDYDADGMSAVAVWAVALRA
                     LGAEAVRHVPSRLAEGYGMSTSGLERLSGLGVTLVVTCDCGVGNVAEVEHARGLGIEV
                     IVTDHHLPGGELPRAVAVVDPHRPDCEYPDPDLTGAGIAYKLASALVKRRGAQVSDLA
                     ALAAIGTVADMAPMTGESRAIVRLGLAEMAATQRAGLRALLARACERPERPTARDLAF
                     TLAPRVNAAGRIDEAELAISLLLEEDPEAAERGAQALEDVHLRRREMTVSAVNSARAM
                     VEGLEGTGPIVVREDSWAPGIIGLVAGRLAEDLSRPVAAAVLVGAEVRGSVRAPDDFH
                     AAHGLEACAHLLTKRGGHAGAGGFSLLPEMWDAFVTAFGALARPYPPGAETVLRVAGR
                     MEVDLVLPARHLGWRLADELDRLAPYGPGHREPLLAITGLRVGSVRRVGADGGHLAFR
                     MLRGAETMDAIAFSMPADRPVPEEGALVDLLGTLERDTFQGQARLRIRVVDYASADAS
                     PLAARRRAAQPTT"
     gene            complement(1819..>2104)
                     /locus_tag="VHK28_03835"
     CDS             complement(1819..>2104)
                     /locus_tag="VHK28_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX2141397.1"
                     /translation="GEPFGAIVNHSILQTLGRSINTSVTVVITLAALLLLGPPSIRTF
                     TLALLIGVVSGTYSSIFNASQLLVAWYEWDARRKARGAGPGTRAVRPLSR"
BASE COUNT          294 a          797 c          753 g          260 t
ORIGIN      
        1 gtcgtgggct gggccgcgcg gcgccgggcg gcgagggggc tggcatcggc ggaggcgtag
       61 tcgactaccc ggattcgcag ccgcgcctgt ccctggaacg tgtcgcgctc cagggtgccc
      121 agcaggtcga ccagggcgcc ctcctcgggc accggccggt ccgccggcat ggagaaggcg
      181 atggcgtcca tggtctccgc gccgcgcagc atgcggaacg cgaggtggcc tccgtccgcg
      241 ccgacccggc ggacggagcc gacgcgcagc ccggtgatgg ccagcagcgg ctcacgatgg
      301 ccggggccgt acggcgccag gcggtcgagc tcgtcggcca gcctccagcc caggtgccgc
      361 gcgggcagga ccaggtccac ctccattcgg cccgcgacgc gcagaacggt ctcagcgccg
      421 ggcgggtagg gccgggccaa cgcaccgaag gcggtcacga aggcatccca catctcgggg
      481 agcaggctga acccgccggc gccggcgtgg ccgccgcgtt tggtcagcag gtgggcgcac
      541 gcctccaggc cgtgggccgc gtggaaatcg tccggggcgc ggaccgagcc gcgtacctcg
      601 gcccccacca gaaccgccgc agccaccggg cgcgagaggt cttccgccag ccggccggcg
      661 accaggccga tgatccccgg cgcccagctg tcctcgcgga ccacgatggg ccccgttccc
      721 tccaggccct ccaccatggc ccgcgccgag ttcaccgccg acaccgtcat ctctcgacga
      781 cgaagatgga cgtcctcgag tgcctgggct ccccgctccg cggcctctgg gtcctcctcc
      841 agcagcaggg agatggcgag ctcggcctcg tcgatacggc ccgctgcatt cacccgcggt
      901 gcgagggtga aggcgaggtc ccgcgccgtc ggtcgctccg gccgctcgca ggcgcgcgcc
      961 agcagggctc ggaggcccgc gcgctgcgtg gctgccatct cggccaggcc gaggcggacg
     1021 atggcgcggc tctcgccggt catgggcgcc atgtccgcca ccgtgccgat ggccgccagc
     1081 gccgccaggt cgctcacctg cgccccacgc cgcttcacga gcgcggacgc gagcttgtag
     1141 gcgatgccgg cgccggtcag atctgggtcg ggatactcgc agtccggccg gtgcgggtcg
     1201 accacggcca ccgcccgcgg cagctcgcct ccgggcaggt ggtggtcggt cacgatgacc
     1261 tcgatcccca gaccccgggc gtgctccacc tctgccacgt tgccgactcc gcaatcgcag
     1321 gtgaccacca gcgtcacgcc caagccgctc agccgctcga gcccgctcgt cgacatgccg
     1381 tagccctccg ccagccggga gggaacgtgc cggacggcct cggcacccag cgcgcgcagc
     1441 gcgaccgccc agaccgccac cgccgacatg ccatccgcgt cgtagtcgcc ccagatggcg
     1501 atccgctcgc cgcggtcgac ggcggtctcc agccggtcca gggcgacgcc ggcatcggcc
     1561 atcagctcga ccgggtgcag ggactgggcc ccggcgccca ggaaggtatc cagctctggc
     1621 tgggtgcgga agccgcgccg cgccagcagg gtggcgacgg gcgggccgaa gccggcgaag
     1681 gccggcgggt caaacaacgg ttcgggcagg agccattcca ggcgcggtcg gatcaccccg
     1741 gcatcgtagc cgcgggccct taccgcccgc ttacgaatcc gttatcagca gcgcggcgcg
     1801 ttcgcgcccg tactccctct atcggctcag cggtcgcacg gctcgcgtgc ccggtcccgc
     1861 accgcgtgcc ttgcgacgcg cgtcccactc gtaccaggcc accagcagct ggctggcgtt
     1921 gaagatggac gagtaggtgc cgctcaccac gccgatcagc agcgccaggg tgaaggtccg
     1981 gatcgacggc gggccgagca acaggagggc cgccagggtg atcaccacgg tgaccgaggt
     2041 gttgatcgag cgacccaggg tctgcaggat cgagtggttc acgatcgccc cgaacggctc
     2101 gccg
//
LOCUS       DATACA010000189         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1792329, whole genome shotgun sequence.
ACCESSION   DATACA010000189 DATACA010000000
VERSION     DATACA010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792329"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VHK28_03840"
     CDS             complement(<1..294)
                     /locus_tag="VHK28_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2141398.1"
                     /translation="MRALLGGKGAGLAEMTNAGLPVPPGFTITTEACNAYYAAGKELP
                     PGLWDGVVAHMKELETQTGKGFGDPARPLLVSVRSGAAFSMPGMMDTVLNLGLN"
     gene            complement(674..>2663)
                     /locus_tag="VHK28_03845"
     CDS             complement(674..>2663)
                     /locus_tag="VHK28_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2141399.1"
                     /translation="EILELKNTINTMVDQLNAFASEVTRVAREVGTEGKLGGQADVRG
                     VGGTWKDLTDSVNLLAANLTTQVRAIGEVATAVTRGDLGRTVEVEAQGEVRILKDDIN
                     EMIRRLRDTTRENAEQVWLKANLARFSSLLQGERDPEKVARMLLSELGPLIGVLTGVF
                     YVAERDGESEQLRLLGSYARGRSPGPPSVVQFGEGLLGQCAVEGRRIVVTRVPAEYSQ
                     ISSALGRAAPSMIVILPVRFEQETMAVIELAAFDSFTQVQLDFLDQLSETVAIVLNTI
                     TANQRTAALVQEQAGRAEAEAGLARLRQVVDVMPEGILIADGNGSVYLHNAAAADIMG
                     MVPESVISPAGLPILRRLDGSVCPPEEQPLARAIFRQEVVRGEQLLVTNAATGREIPI
                     LVNSAPLSDPSRAAAGGVAVFQDIGPLHDLDRQRDQFLAAVSHDLRTPVAVIKGRTDL
                     LQRAVTRSESPDFDQVASGLRTIDESTARLVRLIDELLDLTRIRMGNPVDLQLEPTDL
                     IELTQRITREYDALTADHEIRFDGDVDSLVGEWDAARLERVLANLLSNAVKYSEPDGE
                     IVVHAWREAVDGREWAVLAVRNPGIGIPPGEVDRIFDTYYRGTNVSGSITGTGVGLAG
                     VRHIVEQHGGRIDVDSTQGGTTTFAVRLPLAPPNNGSR"
BASE COUNT          377 a          918 c          909 g          459 t
ORIGIN      
        1 gttgaggcca aggttgagga ccgtgtccat catccccggc atgctgaagg cggccccgga
       61 gcggacgctg accagcagcg gcctggccgg atcaccgaat cccttgccgg tctgggtttc
      121 gagctccttc atgtgcgcca cgacgccgtc ccacagcccg ggcggcagct ccttgccggc
      181 ggcgtagtag gcgttgcagg cctcggtggt gatcgtgaag cccggcggca ccggcagccc
      241 ggcgttggtc atctctgcca ggccggcccc cttgccgccc aggagcgccc gcatctgggc
      301 gttgccctcg gcgaaggtgt agatcagctt gccgtcggcc actgccgtgc cagctgcctt
      361 cgaggtgcgc gtccgcttgc gagtcgtccc ggtcgtgctg gccaatgccc tgtcaggcct
      421 ccgatgttcg ttggggaacc ctcattgtac cgataccgtg gatgggcttc gaaatcggct
      481 ggggggccgg tcgccggcgc cttggacgtc agatagtggc caggtgaatg ccgcgcccag
      541 ctctgcatct gataggccgc cagccattca ccgggtgact gccgtaacgc cccatgagcc
      601 tgatcggctc agtcgagcag aatttcaatg gtttggtagt cgcccgagga atagatggcg
      661 cccgccgcga tgcctagcga gatccgttgt tcggcggcgc caggggcagg cgcacggcga
      721 aggtggtcgt cccgccctgg gtgctgtcca catcgatccg accgccgtgc tgctcgacga
      781 tgtggcgcac gccggccagg ccgacgccgg tgccggtgat cgagccggag acgttcgtcc
      841 cgcggtagta ggtgtcgaag atccgatcga cctccccggg aggaatgccg atccccgggt
      901 tgcgcaccgc cagcaccgcc cactcgcgcc cgtccaccgc ctcgcgccag gcgtgaacga
      961 cgatctcacc gtccggctcg ctgtacttga cggcgttgga cagcaggttg gcgagcacgc
     1021 gctccaggcg cgcggcgtcc cactccccca ccaggctatc cacgtcgccg tcgaagcgga
     1081 tctcgtggtc cgccgtgagt gcgtcgtact cgcgcgtgat ccgttgggtg agctcgatca
     1141 ggtcggtcgg ctcgagctgc agatcgaccg ggttgcccat ccgtatccgg gtcagatcca
     1201 gcagctcgtc gatcagccgg accaggcggg cggtgctctc gtcgatcgtc ctcaggccgc
     1261 tggccacctg gtcgaagtcg ggactctccg atcgcgtcac cgctcgctgc agcaagtccg
     1321 tgcgtccctt gatcacggcg accggggttc gaaggtcgtg cgagacggcc gcaaggaact
     1381 gatcgcgctg tcggtcaaga tcgtggagcg ggccgatgtc ctggaacacc gcgacgccgc
     1441 cggccgccgc gcgcgacggg tcgctgaggg gcgcgctgtt gaccaggatc gggatttcac
     1501 ggcccgtggc cgcgttggtg accaggagct gctcgccgcg gactacctcc tggcggaaga
     1561 tcgcccgtgc caggggttgc tcctcgggag ggcagacgga gccatccagg cgtcgcagga
     1621 ttggaaggcc ggccggcgag atcaccgact cgggcaccat gcccatgatg tcggcggcgg
     1681 cggcgttgtg caggtagacg gacccgtttc catcggcgat gaggatgcct tcgggcatga
     1741 catcgacgac ctgccgcagg cgcgccagcc cggcctcggc ctccgcgcgg ccggcctgtt
     1801 cctggaccag ggccgcggtg cgctggttgg cggtgatggt gttcagcacg atcgccacgg
     1861 tctcgctcag ctggtccagg aagtccagct gaacctgcgt gaagctgtcg aaggcggcca
     1921 gctcgatcac cgccatggtc tcctgctcga atcgcaccgg caggatcacg atcatgctcg
     1981 gcgccgcccg ccccagggcg gagctgatct gcgagtactc ggccggcacg cgggtgacca
     2041 cgatgcgccg cccctcgacc gcacactgcc ccagcaggcc ctcgccgaac tgaacgaccg
     2101 atggtggacc gggtgacctg cccctcgcgt agctgccgag caggcgcagc tgttcggact
     2161 cgccgtcccg ctccgcgacg tagaacaccc cggtcagcac gccgatcagc gggccgagct
     2221 cagacaggag cattcgagcc accttctccg ggtcccgctc accctgcagg aggctgctga
     2281 agcgagcgag gttggccttg agccagacct gctcggcgtt ctcgcgggtc gtgtcgcgga
     2341 ggcggcggat catctcgttg atgtcgtcct tgagaatccg cacttcacct tgcgcctcga
     2401 cctccaccgt gcgtccgagg tctccgcggg tgaccgcggt cgcgacctcg ccgatggcgc
     2461 ggacctgggt cgtgaggttc gccgcgagca ggttcacgct gtcggtcagg tccttccagg
     2521 tgccgcccac gccgcgcacg tcggcctggc cgcccagctt gccctcggtg cccacctcgc
     2581 gcgccacgcg ggtcacctcg ctggcgaagg cgttgagctg gtcgaccatg gtgttgatgg
     2641 tgttcttcag ctccaggatc tcg
//
LOCUS       DATACA010000190         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_506949, whole genome shotgun sequence.
ACCESSION   DATACA010000190 DATACA010000000
VERSION     DATACA010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506949"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1547)
                     /locus_tag="VHK28_03850"
     CDS             complement(222..1547)
                     /locus_tag="VHK28_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141400.1"
                     /translation="MTGHPNETGDSGQRRALPLGQLVTLSIYWLGIQTIWGGVNTVVL
                     PDRFDEMGPAVAGSLLAITLGLSAVAAILVQPTVGIISDYTVTRWGRRKPYIVIGTLL
                     DMVFLVGLATSNTFLSIVAFYFLLQVSSNFAQGPFQGYVPDLVPARQVGLASGLMGLM
                     LTVGTIAGVGLATIGPDLMGQPIAVISLGVVEVLTMVVLVATVNEGRAAPPRPGTWRQ
                     VALSAWARDILGERNVLWLLVVRLLFLGAYNSTLIALLFFQRSLGLPHDEARSTLFVA
                     VAIVAVLTALAAVPGGRLSDRFGRRAVIWGASVIATLGLAAAAVAPTRELAIAAWIPF
                     GIGMGAFLSADWALMADVIPKATAGRYMGILNAGTAMAGPLYIVIAGPAQDLSGALFG
                     VAVGPRVAIGIAALFVVGAALALTRVDPRRREDSMPEAEPAAARAVGAA"
     gene            1705..2589
                     /gene="lgt"
                     /locus_tag="VHK28_03855"
     CDS             1705..2589
                     /gene="lgt"
                     /locus_tag="VHK28_03855"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX2141401.1"
                     /translation="MIDPILFQIGPFQVHWYGVIIASAVLLAGVVGSMEARRRGEDPE
                     VGWSMLLPVLILGVLGARLYHVIHEWSFYSQNLALIPQVWLGGLGIPGAVVGGIVAIW
                     LYCRINGLNTLRWFDIFATALLLGQAIGRLGNFVNQELYGPPTTLPWGIPIDADHRVG
                     EWMNLAAYPVETTRFHPLFAYEGLLNLVGLALILWIGRRFAHRLYDGDILLIYLMWYG
                     AVRTALEPFRTDNWIILGVPTAIWIGVLSFIGAGFLLWLRHRRGWGTPGAWMRERRGT
                     DVDAEPGDTQASPEPSAG"
     gene            2534..>3557
                     /locus_tag="VHK28_03860"
     CDS             2534..>3557
                     /locus_tag="VHK28_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2141402.1"
                     /translation="MWTPSQAIPRPPRSQALADRGGILPDVTAAIGDTPLVALDRLAA
                     GVAPRLVAKLEHMNPGGSVKDRIALPMIEAAERAGMLKPGGTIVEPTSGNTGVGLAQA
                     AAVKGYRCIFVMADKQSEEKRALLRAYGAEVVVCPTDVAPDDERSYYRVSDRMAREIP
                     GAWKPDQYSNPANPEAHYLTTGPEIWEATDGRITHFVVALGTGGTVTGTGRYLKERNP
                     SVIIVGADPEGSVYSGADPHPYLTEGIGEDFWPGTYDASMCDVVIRVSDRDSMLMARA
                     ATATEGILMGESSGTAIWAALQHAREVDDREALFVVLLPDSGRNYLGKLYDDAWLREK
                     GLLGVDE"
BASE COUNT          570 a         1201 c         1258 g          528 t
ORIGIN      
        1 gaccgccatg accagcagtg ggtcccagcc gttgtggtgg ttggcgacca ggcagaacgg
       61 cgccgaaggc caccgcttga gtccgctcgc gtgcacgtcg aagaggaggc gcaccaaggt
      121 ccggttcgcc ccctacagca gccgatagcg ccggtcgggg cgaacggaga gctcgcgcgg
      181 gccggtccgc cgggcgcggt ccgccgggct ggaggctgcg ctcacgccgc gccgaccgcc
      241 cgggcggccg ccggctctgc ctctggcatt gaatcttccc gacgccgggg atcgacgcgc
      301 gtgagggcca gcgcggcccc caccacgaac agcgccgcga tcccgatcgc cacccgcgga
      361 ccgacggcca ccccgaagag ggcacccgac aggtcctgtg ccggtccggc gatcacgatg
      421 tacagcgggc cggccatggc ggtcccagcg ttgaggatgc ccatgtagcg accggcggtg
      481 gcctttggga tgacatcggc catgagcgcc cagtccgcgg agaggaacgc gcccatcccg
      541 atcccgaacg gtatccaggc cgcgatggcc agctcacgcg tgggggccac ggcggcggcc
      601 gccaggccga gcgtggcgat gacggacgcg ccccagatga ccgcgcgacg gccgaaccga
      661 tccgagaggc gaccaccggg caccgcggcc agcgcggtga gcaccgccac gatggcgacc
      721 gccacgaaca gcgttgaccg tgcctcgtcg tgcggcaggc cgagcgaccg ttggaagaag
      781 agcagcgcga tcagcgtgct gttgtaggcg cccaggaaca gcagacgaac gacgagcagc
      841 cacagcacgt ttcgttcgcc caggatgtcc cgagcccagg cggagagcgc cacctgtcgc
      901 cacgttccgg gcctcggcgg agcagccctg ccctcgttca cggtcgccac caggaccacc
      961 atggtgagca cctcgaccac ccccaggctg atgaccgcga tcggctgacc catgaggtcc
     1021 ggaccgatgg tggccaggcc cacaccggcg atggtgccga cggtcagcat cagtcccatc
     1081 agcccggacg ccagtccgac ctggcgcgcc gggacgaggt ccggcacgta tccctggaac
     1141 ggaccctgcg cgaagttgga gctgacctgc agcaggaagt agaaggccac gatcgacagg
     1201 aacgtgttcg acgtggccag cccgaccagg aagaccatgt cgagcagcgt gccgatgacg
     1261 atgtagggct tgcggcgacc ccagcgcgtc acggtgtagt cgctgatgat cccgaccgtc
     1321 ggctgcacca ggatggcggc caccgcactc aggcccaggg tgatggcgag cagcgagccg
     1381 gcgacggcag ggcccatctc gtcgaagcga tcgggcagca ccacggtgtt cacgccgccc
     1441 cagatggtct ggatgccgag ccagtagatg gacagcgtca ccagctgccc caatggaagc
     1501 gcccgccgtt ggccgctgtc gccggtttcg ttcgggtggc cagtcatctc gcttctccgg
     1561 ggaggccgtt catcggtcac cgcactcggc acgatacgcg tggccggagc tcggtgcgat
     1621 cggattgtgt gacgcctgcc ctgcggaagg actgcggaga cggcgagtgc gcggcagccg
     1681 gaatgggcgg gtatgatcgg ccgagtgatc gatcccatcc tctttcagat cggcccgttc
     1741 caggtgcact ggtacggggt catcatcgcc tccgccgtgc tgctggcggg cgtcgtcggc
     1801 tccatggagg cgcgccggcg gggcgaggac cccgaggtgg gctggtccat gctgctgccg
     1861 gtcctgatcc tgggtgtcct gggcgcgcgc ctgtaccacg tcatccacga gtggagcttc
     1921 tactcgcaga accttgccct cattccccag gtgtggctcg gcgggctggg catccccggc
     1981 gcagtggtgg gtggcatcgt ggccatctgg ctctattgcc gcatcaatgg actgaacacg
     2041 ctgcgctggt tcgacatctt cgccaccgcg ctgctcctgg gccaggccat cggtcgcctg
     2101 ggcaacttcg tgaaccagga gctctacggc ccgccgacca cgctgccgtg gggcattccc
     2161 atcgacgcgg atcatcgcgt gggggagtgg atgaacctgg cggcgtaccc ggtggagacc
     2221 acccgcttcc atccgctctt cgcctacgag ggcctgctca acctggtggg gctggcgctc
     2281 atcctgtgga tcgggcggcg cttcgcccat cggctctacg acggcgacat cctgctcatc
     2341 tacctcatgt ggtacggcgc cgtgcggacg gcgcttgagc cgtttcgaac cgacaactgg
     2401 atcatcctgg gtgtgcccac cgccatctgg atcggcgtgc tgagcttcat cggtgccgga
     2461 ttcctgctct ggctccggca ccgcagggga tgggggactc ctggagcctg gatgcgcgaa
     2521 cgtcgcggga ccgatgtgga cgccgagcca ggcgataccc aggcctcccc ggagccaagc
     2581 gctggctgac cgcggcggca tcctgcccga tgtcaccgcg gccatcggcg acaccccgct
     2641 ggtggccctc gaccggctgg cagccggcgt cgcgccgcga ttggtcgcca agctggagca
     2701 catgaacccg ggcggttcgg tcaaggaccg catcgcgctg cccatgattg aggccgccga
     2761 acgagccgga atgctgaagc cgggcggaac gatcgtggag cccaccagcg gcaacaccgg
     2821 ggtggggctg gcgcaggcgg cggcggtgaa gggctatcgg tgcatcttcg tcatggccga
     2881 taagcagtcg gaggaaaagc gcgcgctgct gcgggcctat ggtgcggagg tggtggtctg
     2941 tcccaccgac gtggcgccgg atgacgagcg ctcctactac cgtgtgtccg accgcatggc
     3001 gcgcgagatc ccgggtgcct ggaagccgga ccagtactcg aacccggcca acccggaggc
     3061 gcactatctg accaccggcc ccgagatctg ggaggcgact gacgggcgca tcacgcactt
     3121 cgtggtcgcc ctcggaacgg gcgggacggt taccggcacg gggcgctacc tgaaggagcg
     3181 caacccttcg gtgatcatcg tgggcgcgga tcctgagggg tcggtgtact ccggcgcgga
     3241 cccgcatccc tacctgacgg agggcatcgg cgaggacttc tggccgggga cctacgacgc
     3301 gagcatgtgc gacgtggtga tccgcgtctc cgaccgggac tcgatgctga tggctcgcgc
     3361 cgccaccgcc actgagggga tcctgatggg ggagtcatcc ggaactgcca tatgggcggc
     3421 gctgcagcac gcacgcgagg tggatgatcg cgaggcgctg ttcgtcgtcc tcctgccgga
     3481 cagcggccgc aactacctgg gcaagctgta cgacgacgcc tggctgcgcg agaagggcct
     3541 gctgggcgtc gacgagg
//
LOCUS       DATACA010000191         8164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_748573, whole genome shotgun sequence.
ACCESSION   DATACA010000191 DATACA010000000
VERSION     DATACA010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748573"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /locus_tag="VHK28_03865"
     CDS             complement(<1..1338)
                     /locus_tag="VHK28_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2141403.1"
                     /translation="MDLGRPSIRLFAALLVVALPPLVAFAALRVFAADWLLARGFGAG
                     TQLLIGAAVTVAWAALVAVAAARLMAGEARSMLELAERGITSGAEREDDLSEAQRRLA
                     TTLEERNRQIANLAEMVRSAPMVEDPASVARSMADAARQLTSDPTWVLVVLRGPDDRS
                     LPPGVYAPAPAEAVEPVGEVHRWASTVEAAPHGARHAIGPWGAFVVVDVAAGEELRAI
                     LMAPREGRPAPPPAELALLSLLGQHAATAVEHALLYTQLRAQADQLNRMAAVQSDFLR
                     GVTHDLQTPLTSIRALASELRNSVGTDAAAQTDLDTITHQADRLRRMVGQLLAVSRLE
                     AGALTPSLDVFRVEPLVRRTWEALRADRPFTVEVEGTEHLVVGDPDRVEQVLWALLDN
                     GVKYSPAGSPITLRIRGDASGSDLRSVIEIADQGAGMDAATLGRAFEQFYRSAD"
     gene            complement(1343..2071)
                     /locus_tag="VHK28_03870"
     CDS             complement(1343..2071)
                     /locus_tag="VHK28_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2141404.1"
                     /translation="MQPALRPAVLLVDDDPTLLSVLSRRLSREGLEVRTASSGSAALA
                     ELDRAWPALLVVDLMMPGMDGFELCRRVKRIADLPIIVLSAVDASESKVRALEEYAED
                     YITKPFNPDELVARVSRVLRRATAGVSQVTLAGGELEIDLTGRRIVTRSGSHVLTPIE
                     VRLLQVLIASLDRTVATETLLDRVWSEADGADPSYVWVSVRRLRRKLEVDPDRPRFLL
                     TERGIGYRLVSGSTASDASVAGRG"
     gene            complement(2088..3011)
                     /locus_tag="VHK28_03875"
     CDS             complement(2088..3011)
                     /locus_tag="VHK28_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2141405.1"
                     /translation="MPEIPTEILIALFAALAVLLITYGLAASPPKDAVQTRLEQLVVQ
                     PKTLEEFELQQPFFERVVRPVVKRLSKVGRRGDQGGLIARTDAKLEKAGYPGGLRGAD
                     WMGVKILSTIVVTLAGFFLGLAMGGFPMAMLFTLVGAMFGFIGPEFWLGRKIRARSMA
                     MVLQLPDALDLLTISVEAGLGFDAALAKVVEKMEGPLVNEFRQALAEIRMGRSRRDAL
                     RDVVTRADAQPVSNFIGAIVQAEQLGVPIAKVLQIQSQQLRIERRQRAEEAAAKAPVK
                     MLFPMVGCIFPTIFIVILGPAIVTVMGGVGI"
     gene            complement(3016..4005)
                     /locus_tag="VHK28_03880"
     CDS             complement(3016..4005)
                     /locus_tag="VHK28_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2141406.1"
                     /translation="MGMLQFVIAGLAAGAVLLIAIGVAMSGGSGGVAARLERYARPEE
                     GDAADGQDRQGVVTAGLNRVIERQGLAGRLSTEIARADLKLRPAELVAAWIASPFVFL
                     GAGFLLGLFLPGFQNPLALAGMFLIGAFFPRFYLKYRQGKRLQAFASQLPDTITLLAN
                     SLRAGSSFLQGVELVTREARPPISEEFERVVREMNLGVALQPALNNLVRRVASEDLEL
                     MVTAINIQSQVGGNLATVLDSIAYTIRERIRIQGEIKTLTAMQRYSGYVITLLPVGLA
                     GILFLISPSYISRMLDKPPEMFGLPVGVVFFIIGGISMFIGYLLIRRVVDIKV"
     gene            complement(4022..5458)
                     /locus_tag="VHK28_03885"
     CDS             complement(4022..5458)
                     /locus_tag="VHK28_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX2141407.1"
                     /translation="MSLLKRIERARPAAEGEQPNVPATAPQTPAAPPRPGAPVTPPPP
                     GGQPAGRPFLGALPARESFREAKFRVQNRLINELDPKLDLTNQVEVRRQIEELFGKIA
                     DEEGLALTRAERVRMLEQITDEILGLGPLEPLLRDETITEVMVNGPQQVYIEREGRLE
                     LTNVTFQNDEHVMKIIQRIIAPIGRRVDESSPMVDARLADGSRVNAIIPPLSLVGPVV
                     TIRKFSATPFTVEDLIRFGTATPEMFEFLEACVKARLNVFVSGGTGSGKTTMLNILSS
                     FIPDDERIVTIEDAAELQLRQEHVITLESRPPNIEGKGAIPIRELVRNALRMRPDRIV
                     VGECRSGEALDMLQAMNTGHDGSMSTGHANTPRDMLSRLETMVLMAGMELPLRAIREQ
                     IASAVDLIVHQNRLKDGSRKIVAITEVQGMEGDVIVMQDIFVFEQTAVVEGKIQGRLK
                     PTGIRPKFVEKFEVAGIHLPPNVFGSPF"
     gene            complement(5478..6701)
                     /locus_tag="VHK28_03890"
     CDS             complement(5478..6701)
                     /locus_tag="VHK28_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141408.1"
                     /translation="MIKVLIVDDIPETRDHLVRLLGLEQEIEVAGVAGTGEEAIKQSL
                     DLHPDVVVMDINMPGMDGVAAAEIITQRLPNTPIIMMSVHGEAEQLKRSLSAGAREFL
                     VKPFSADELSTTIRRVYERELVRRDQLAATVAPATQAVAPLGEELGEHQVIAVFSPKG
                     GAGRTTVATNLAVALQRETGGRICLVDANLQFGDVGVLLNLNPKNRSMLDAVEGGEPD
                     ADIINSVVIDHSTGIRVLLAPPSPEGADLVTPAYLRKMVDTLREGHNYVVVDVPATLH
                     DHTLAVLDAADQIVVLTALEITTIKNVRLFLEVADQLEYDRSKIRLVVNRSDSAQGIR
                     IGDVEASIRRSIDGTVVSDGRLAVLAVNRGVPFVVSHPEGALSRDIIKLARTLAGDAV
                     AANGKPNKRGLFARR"
     gene            complement(6740..7633)
                     /gene="cpaB"
                     /locus_tag="VHK28_03895"
     CDS             complement(6740..7633)
                     /gene="cpaB"
                     /locus_tag="VHK28_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX2141409.1"
                     /translation="MKRSNRLVILVGVLLAVLAFVGIVIILNQQQQPEEVEQTTVPVL
                     VAVEDIAIGDEVTPDLVEIEEVQPDQVVGTRIGSTTQVSGQRALYDIPAGSQVPAEAI
                     GLGQVGDVCIECQLNPGEKAVAFQVDRVTGLDFLIQPGDHVDIVVSQNLQVVQPTADS
                     LEDPTGPQRFETIPGLENSRTVKTILQDKRVLYVSATRIRSTAVQETPAPGEDAPPAQ
                     PAQQIENVIIVFAGTDQDAEVIKFAQNDVNNVGPLTAVLRSTDDTDVEVTTGITIDLL
                     VENYGLPIPDIIADLGEEAAP"
     gene            complement(7862..8047)
                     /locus_tag="VHK28_03900"
     CDS             complement(7862..8047)
                     /locus_tag="VHK28_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX2141410.1"
                     /translation="MLQFISTLLARFRREDEEGQTLVEYGLLLALIAIIVIVALIFLG
                     PIVSQIFQNVGQNLQTT"
BASE COUNT         1365 a         2703 c         2786 g         1310 t
ORIGIN      
        1 atcggccgag cggtagaact gctcgaacgc ccggcccagc gtggccgcat ccatgccggc
       61 tccctggtcg gcgatctcga tcacggaacg caggtcgctc cctgacgcat ctcccctgat
      121 gcgcagggtg atgggtgaac cggccgggct gtacttgacc ccgttgtcca gcaacgccca
      181 gagcacctgc tcgacccggt cggggtcccc caccaccagg tgctccgtac cttcgacctc
      241 caccgtgaac ggccggtcgg cgcgcagcgc ctcccacgtg cgccgcacca gcggctcgac
      301 ccggaacacg tcgagcgacg gcgtcagcgc cccggcctcc aggcgcgaga ccgccaggag
      361 ctggccgacc atgcgtcgca ggcgatccgc ctggtgggtg atggtgtcaa ggtctgtctg
      421 ggcggccgca tcggtcccta ccgagttgcg gagctcggag gcgagggcac ggatgctggt
      481 cagcggcgtc tggaggtcat gtgtcactcc ccgcaggaag tccgactgga cagcggccat
      541 cctgttgagc tggtccgcct gggcgcggag ctgcgtgtag agcagggcat gctcgacggc
      601 ggtggcggcg tgctggccca ggaggcttag gagcgccagc tcggcggggg gaggagcggg
      661 acgaccctcc cgaggcgcca tcaggatggc gcgcagctcc tcaccggccg ccacatccac
      721 caccacgaag gctccccagg gaccgatggc gtggcgtgcc ccgtgcggtg cggcttccac
      781 cgtggatgcc cagcgatgca cctcgccaac gggctcgacc gcctccgcag gtgctggcgc
      841 gtagaccccc ggcggcaggg agcggtcgtc ggggccacgg agcacgacca gaacccaggt
      901 cgggtccgag gtgagctgcc gtgcggcatc ggccatggat cgggcaactg acgcaggatc
      961 ctccaccatt ggcgcgctgc ggaccatctc ggccaggttg gcgatctggc ggttgcgctc
     1021 ctccagggtg gtggccaggc ggcgctgggc ctcgctgagg tcgtcctcgc gctccgcacc
     1081 cgacgtgatg ccccgctcag ccagctcgag catggatcgg gcttcgccgg ccatgaggcg
     1141 agcggccgcc acggccacca gtgcggccca agcaaccgtg accgccgcgc cgatcagcag
     1201 ctgcgtgcct gccccgaaac cgcgtgccag cagccagtcg gcggcgaaga cgcgcagggc
     1261 agcgaaggcc accagcggcg gaagggcgac caccagcagc gcagcaaaga ggcggatcga
     1321 cggacggccg aggtccatcc gatcagccgc ggcccgcgac gctggcgtcg ctcgcggtcg
     1381 agccggaaac cagccggtat ccgatccccc gctcggtgag caggaagcgc gggcggtccg
     1441 ggtccacctc cagcttgcgt cgcagacggc ggacgctgac ccagacgtag gatggatcgg
     1501 ccccgtcggc ctcggaccag acccggtcca gcagcgtctc ggtggcgacg gtgcggtcga
     1561 gcgaggcgat cagcacctgg agcagacgaa cctcgatggg cgtcaggacg tggctcccgg
     1621 aacgggtcac gatccggcgt ccggtcagat cgatctccag ctcgccgccg gccaacgtga
     1681 cctgcgacac gccagcggtt gcacgtcgca gcacgcggct gacgcgggca accagctcat
     1741 cggggttgaa cggcttggtg atgtagtcct cggcgtactc ctcgagcgcg cggaccttcg
     1801 actcggaggc atccaccgcg gacagcacga tgatcggcag gtcggcaatg cgcttcacac
     1861 gacggcacag ctcgaagccg tccatgcccg gcatcatgag gtccacgacc aggagggcgg
     1921 gccaggcgcg gtccagctcg gccagtgcag cggaaccgga tgaggcggta cggacctcca
     1981 gtccctcgcg gctcaggcgc cgcgagagga cggacaggag cgtcgggtcg tcgtcgacca
     2041 gcaggacggc cggccgcagc gccggctgca tctcgtgtgc cggcgcctca gattccgacg
     2101 ccgcccatga cggtcacgat cgccggtcca aggatcacga tgaagatcgt ggggaagatg
     2161 cagcccacca tggggaacag catcttgacc ggggccttgg cggcagcctc ctccgcccgc
     2221 tggcgacgct cgatgcgcag ctgctgggac tggatctgca gaaccttggc gatcgggacg
     2281 ccgagctgct cggcctggac gatggcgccg atgaagttcg acaccggctg ggcgtccgcc
     2341 cgcgtcacca cgtcgcgcag ggcatcgcgc cggctgcggc ccatccggat ctcggcgagt
     2401 gcctggcgga actcgttgac gagggggcct tccatcttct ccacgacctt ggccagtgcc
     2461 gcgtcgaagc cgaggccagc ctccacggag atggtcagca ggtcgagggc gtcgggcagc
     2521 tggaggacca tggccatcga gcgcgcgcgg atcttgcggc cgagccagaa ctcgggaccg
     2581 atgaagccga acatggcgcc gaccagggtg aagagcatgg ccatcgggaa gccgcccatg
     2641 gccaggccca ggaagaagcc ggccagggtg acgacgatgg tcgacaggat cttgacgccc
     2701 atccagtcgg caccgcgcaa gccgcccggg tagccggctt tctccagctt cgcgtcggtc
     2761 cgggcgatca ggccgccctg gtctcctcgg cggcccacct tggacaggcg cttgacgacg
     2821 ggtcgcacca cgcgctcgaa gaacggctgc tggagctcga actcctccag cgtcttgggc
     2881 tggacgacga gctgctccaa cctcgtctgg acggcatcct tgggcgggga tgccgccaat
     2941 ccatacgtga tcagcaggac ggcgagcgct gcgaagagcg ctatgaggat ctcggtggga
     3001 atctcgggca tggcgctaca ccttgatatc gacgacgcga cggatcagca ggtatccgat
     3061 gaacatgctg atgccaccga tgatgaagaa cacgacgccg accggcagcc cgaacatctc
     3121 cggcggcttg tcgagcatgc gggagatgta cgaggggctg atcaggaaca ggatccccgc
     3181 caggcccacc ggcagcaggg tgatgacgta gccgctgtag cgctgcatgg cggtcagggt
     3241 cttgatctcc ccctggatcc ggatgcgctc gcggatggtg taggcgatgg aatccagcac
     3301 ggtggccaag ttgccaccaa cctggctctg gatgttgatg gcggtgacca tcagctccag
     3361 gtcctccgac gccacgcgcc ggaccaggtt gttgagggcg ggctgcagcg ccacgcccag
     3421 gttcatctcg cgcacgaccc gctcgaactc ctctgagatc ggcggccgcg cctcgcgcgt
     3481 gaccagctcg acgccctgga ggaaggatga cccggcccgt agcgagttgg ccagcagggt
     3541 gatggtgtcg ggcaattgag aagcgaacgc ctgcagccgc ttgccctgcc ggtacttgag
     3601 gtagaagcgg ggaaagaatg caccgatcag gaacatcccg gccagcgcca gcgggttctg
     3661 gaagccgggc aggaacagcc cgagcaagaa gccagcaccc aggaacacga atggcgaggc
     3721 gatccatgct gccaccagct cggccggccg cagcttgagg tccgcgcggg cgatctccgt
     3781 ggacaggcgg ccggccaggc cctggcgctc gatgactcgg ttgaggccgg ccgtgaccac
     3841 cccttggcgg tcctggccat ccgctgcgtc accctcttcc gggcgcgcgt agcgctccag
     3901 ccgagccgcc acgccgcctg accccccgct catggcgacg ccaatggcga tcaggagcac
     3961 ggctcctgcc gccaggccgg cgatcacgaa ctgaagcatg cccatctcga gcgatcctct
     4021 cctagaacgg cgagccgaag acgttcggcg gcaggtgaat tccggccact tcgaacttct
     4081 cgacgaactt gggccgaatg ccggtcggct tcaggcggcc ctggatcttg ccctccacga
     4141 cggcagtctg ctcgaacacg aagatgtcct gcatgacgat cacgtcgccc tccattccct
     4201 gcacctcggt gatcgccacg atcttgcgcg atccatcctt gagccggttc tggtggacga
     4261 tgaggtcgac ggccgatgcg atctgctcac ggatggcccg cagcggcagc tccatgccgg
     4321 ccatcagcac catggtctcg agccgcgaga gcatgtcgcg cggcgtgttg gcgtggccgg
     4381 tggacatcga gccgtcatgg ccggtgttca tggcctgcag catgtccagc gcctcgccgg
     4441 accggcactc ccccacaacg atgcggtcgg gccgcatgcg cagggcgttg cgcaccagct
     4501 cacggatggg gatggcgccc ttgccctcga tgttcggcgg gcgggactcc agggtgatga
     4561 cgtgctcctg gcgcaactgg agctcggcgg cgtcctcgat ggtcacgatc cgctcgtcgt
     4621 cggggatgaa cgaggagagg atgttcagca tcgtggtctt gccggagccg gtgccgccgg
     4681 agacgaagac gttgagcctg gccttcacgc aggcctccag gaactcgaac atctccgggg
     4741 tggcggtgcc gaagcggatc aggtcctcca cggtgaacgg cgtggcggag aactttcgga
     4801 tggtgaccac cggtcccaca agggagagcg gcggaatgat ggcgttcacg cgggagccat
     4861 cggccaggcg ggcgtccacc atcgggctcg actcgtccac gcggcgcccg atgggagcga
     4921 tgatgcgctg aatgatcttc atcacgtgct cgtcgttctg gaacgtcacg ttggtgagct
     4981 ccaggcgacc ctcacgctcg atgtagacct gctgcggccc gttgaccatc acctcggtga
     5041 tcgtctcgtc gcgcaggagc ggctccaggg ggccgaggcc aaggatctcg tcggtgatct
     5101 gctcgagcat gcggacacgc tcggcgcggg tcagggccag gccctcctcg tcggcgatct
     5161 tgccgaagag ctcctcgatc tgacgccgga cctcgacctg gttcgtcagg tcgagcttcg
     5221 ggtccagctc attgatgagc cggttctgga cgcggaactt ggcctcccgg aacgactcac
     5281 gggccggcag cgctcccagg aacgggcgtc ctgcgggctg acctccgggc ggcggtggcg
     5341 tgaccggcgc accagggcgc ggcggggccg ccggcgtctg cggcgcagtt gccggaacgt
     5401 tcggctgctc cccctctgcg gcgggccgcg cccgctcgat cctcttcaac aatgacatga
     5461 cgacttgatc cttcccctta acggcgtgcg aagagtcctc gcttgttggg cttgccattg
     5521 gcagcgacgg cgtcgcccgc aagcgttcga gcgagcttga tgatgtcgcg cgagagcgcg
     5581 ccttctggat gcgatacgac gaacggcacg ccccgattca cggcgagcac cgccagccga
     5641 ccatcggaga cgaccgtgcc gtcgatgctt cgacgaatgg acgcctccac gtcccctatc
     5701 cggatgccct gcgccgagtc ggagcggttg acgacgagcc gaatcttcga ccggtcgtac
     5761 tcgagctgat cggccacctc caggaagagg cggacgtttt tgatggtcgt gatctccaaa
     5821 gcggtgagca ccacgatctg atcagcggcg tcgagcaccg ccagggtgtg atcgtggagc
     5881 gtggccggaa cgtcgaccac cacgtagttg tgcccctcgc gcagggtatc caccatcttg
     5941 cgcaggtagg caggcgtcac caggtccgcg ccctcagggg acggcggtgc caggagtacc
     6001 cggatcccgg tcgaatggtc gatgacgacc gaattgatga tgtcagcatc cggctcgccc
     6061 ccctcgaccg cgtcgagcat ggaccgattc ttcggattca ggttcaggag cacgcccacg
     6121 tcgccgaact ggaggttggc gtccacgagg cagatgcgtc ccccggtctc gcgctgcagc
     6181 gccacggcca ggttggtggc caccgtggtg cgcccggccc cacccttcgg cgagaagacg
     6241 gcaatcacct ggtgctcgcc cagctcctcc cccagcggtg ccacggcctg ggtggccggt
     6301 gcgaccgtgg cggccagctg atcgcgccgc acgagttcgc gctcgtagac gcgtcgaatg
     6361 gtcgtggaca gctcgtccgc ggagaagggc ttgaccagga actcacgggc accggcggac
     6421 agcgatcgct tcaactgctc cgcctcgccg tgcacgctca tcatgatgat cggcgtgttg
     6481 gggagccgct gggtgatgat ctccgcagcg gccaccccgt ccatgccggg catattgatg
     6541 tccatgacga cgacgtccgg atggagatcc agcgactgct tgatcgcctc ctcccccgtt
     6601 ccggccaccc cggcgacctc gatctcctgc tccaggccaa gcagccggac caggtggtcg
     6661 cgggtctccg ggatgtcgtc gacgatgagg accttgatca cgttggctcc ttcgggaacg
     6721 aaagctgcgc gggtatcggc tagggagccg cttcctcgcc caggtcggcg atgatgtccg
     6781 gaatcggcag gccgtagttc tcgaccagca agtcgatggt gataccggtg gtgacctcga
     6841 cgtcggtgtc atcggtcgat cgcagcaccg cggtcagcgg gccgacgttg ttcacgtcgt
     6901 tctgcgcgaa cttgatgacc tccgcgtcct ggtccgtccc ggcgaagacg atgatcacgt
     6961 tctcgatctg ctgggccggc tgggcaggtg gcgcatcctc gcccggggcg ggcgtctcct
     7021 gtacggccgt gctccgaatg cgggtggcgc tgacgtacag cactcgcttg tcctggagga
     7081 tggtcttcac ggtccgggag ttctccaggc cggggatcgt ctcgaagcgc tgcgggccag
     7141 tcgggtcttc cagcgagtcc gcggtgggct gaaccacctg caggttctgg ctgaccacga
     7201 tgtcgacgtg gtcgccgggc tggatcagga agtccaggcc ggtgacgcgg tccacctgga
     7261 aggcgactgc cttctcgccc gggttcagct ggcactcgat gcacacgtcg ccgacctgtc
     7321 ccaaaccgat tgcctcggcc gggacctggg aaccggcggg gatgtcatag agcgcgcgct
     7381 ggccggagac ctgcgtcgtg ctgccgatgc gggtgccgac gacctggtcg ggctgcactt
     7441 cctcgatctc gacgagatcg ggtgtgacct cgtcaccgat ggcaatgtcc tcgacggcaa
     7501 ccagcaccgg cacggtcgtc tgctcgactt cctccggctg ctgctgctga ttgaggatga
     7561 tcacgatgcc gacgaacgcc aggacggcga gcagcacgcc gaccaggatg accaggcgat
     7621 tggatcgctt cacgaaacgg accccttagc tcggaggaaa tgaaatcaat gaggacgcga
     7681 aatgtgcagc cattgtgact gcggtcaaca gtctacgagc ggtttgcaag cgccagcgag
     7741 gggacttgtg ggctaggccg atgggggtac tcgcgctcga ggcgctcccc tggcagcgcg
     7801 aaaggaggag cccccgcctt gacggcaggg gctcctcgca tcgtcgatgc agtgtgcaga
     7861 gttacgtggt ctgcaggttc tgtccgacgt tctggaagat ctggctcacg attgggccga
     7921 ggaagatgag ggccacgatc acgatgatcg cgatgagggc cagcagcaga ccgtactcga
     7981 cgagcgtctg accttcctca tcctcgcggc ggaagcgtgc aagaagagtg ctaatgaact
     8041 gaagcatcct cgcgattacc tcctttcctc aagaatttga cggcagcgta ggttgcctga
     8101 tcagggcatg aaatgacccg atggtcccaa ctaccgccgt tgggctccgt accatctatc
     8161 gtcc
//
LOCUS       DATACA010000192         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1631857, whole genome shotgun sequence.
ACCESSION   DATACA010000192 DATACA010000000
VERSION     DATACA010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631857"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHK28_03905"
     CDS             <1..358
                     /locus_tag="VHK28_03905"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017025356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2141411.1"
                     /translation="GLTSEVVDRQMRAAISGLDRIEGSGLVIAYEPVWAIGTGLAATP
                     DDAQTAAAQIRAILHEADPDGAHDVPILYGGSCTPENAAEIFAQPDVDGALVGGASLD
                     ADRFAAIVRAAAERAR"
     gene            355..1899
                     /gene="gpmI"
                     /locus_tag="VHK28_03910"
     CDS             355..1899
                     /gene="gpmI"
                     /locus_tag="VHK28_03910"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX2141412.1"
                     /translation="MTDGRPAQRPVVLCVLDGFGLNDDPRRNALLAARMPAWRALTAS
                     WPACRLGASGEAVGLPAGQMGNSEVGHLNLGAGFPVLQDLPRISKAVADGTFFDNPVL
                     REAIETALRRGSRVHLMGLIGPGGIHALDEHIEAMAELAYRAGLPPDRVLLHAFTDGR
                     DTSPRSADVFLPALESRLAGRAIVATVSGRFYAMDRDRRWERTALAWEALVHGAGERA
                     AGGTQAIAAAYARGEGDEFIRPTVVDTYDGMRDSDVAVHLNFRADRARQLTRALALDG
                     FDGFDRGRRPAELYVATLTEYQARDELPVPVAFPPLEIDSLAAYLSRLGRRQLHLAET
                     EKYAHVTYFFNGGVEAQLPGEERRLIPSRRDVPTYDLAPEMSAPGITDALLEAIGSGT
                     YDFIIVNYANPDMVGHTGVWDAAVQAAEVVDGCLDRVSSAVLAAGGALVITADHGNIE
                     EMRDEAGAPQTKHTTSPVPLLLVAEAWRAARLRDGILADVAPTLCELMGIEVPPQMSG
                     RSLILR"
     gene            1952..2176
                     /gene="secG"
                     /locus_tag="VHK28_03915"
     CDS             1952..2176
                     /gene="secG"
                     /locus_tag="VHK28_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX2141413.1"
                     /translation="MTPLVVAQMILAVALIASILLQQRGTGLGGAFGGEVTAYRSRRG
                     IERTLFRLTIVLAVLFVLFSLLNLVDFGAL"
     gene            2227..2313
                     /locus_tag="VHK28_03920"
     tRNA            2227..2313
                     /locus_tag="VHK28_03920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2261..2263,aa:Leu,seq:cag)
     gene            2321..2405
                     /locus_tag="VHK28_03925"
     tRNA            2321..2405
                     /locus_tag="VHK28_03925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2355..2357,aa:Leu,seq:gag)
     gene            2784..3737
                     /locus_tag="VHK28_03930"
     CDS             2784..3737
                     /locus_tag="VHK28_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141414.1"
                     /translation="MVAIAGSQRRVGIATVLLLLGLVLSMFATGRPIVNVSQASAHAP
                     LTADWSCNGTRYFNDHRSVGQTITSGQTIDGGTIVIEGDSFMEMRDNPAGGSDPMHGH
                     IVAEFDRPGVTGNDTATITFDRNIQITSIYLWDNDPAAGETGWSFQGSLLTQTGHQNA
                     LIIPWNETTNTVTFSAGLDSGGIDFCYVEVPNGGGTEGCTPGYWKQAHHFDSWVTYVP
                     TQTLESVFDVPDSLGMDNFTLLQALNFGGGGGVEGMARNLLRHAVASLLNGSNPNVDS
                     PLTDAEVISATNTALASLNRGTMETQKNIFEDNNELGCPLN"
     gene            3837..3913
                     /locus_tag="VHK28_03935"
     tRNA            3837..3913
                     /locus_tag="VHK28_03935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3871..3873,aa:Val,seq:tac)
     gene            4059..4247
                     /locus_tag="VHK28_03940"
     CDS             4059..4247
                     /locus_tag="VHK28_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX2141415.1"
                     /translation="MVDEAWNQISVQLKRRHDLIPNLVNAVKGYMDFEQETLTRVIEA
                     RNQAVSAQEAGPPGSGPR"
     gene            complement(4222..4348)
                     /locus_tag="VHK28_03945"
                     /pseudo
     CDS             complement(4222..4348)
                     /locus_tag="VHK28_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011589187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
BASE COUNT          680 a         1505 c         1449 g          714 t
ORIGIN      
        1 gggacttacg tccgaggtgg tggaccgaca gatgcgtgcc gccatctccg ggctcgaccg
       61 gatcgagggc agcgggctgg tgatcgcgta cgagccggtg tgggccatcg gcaccggctt
      121 ggcggccacg ccagacgatg cgcaaacggc ggcagcgcag atccgcgcaa tcctccacga
      181 ggcggacccc gacggcgcgc acgacgtacc gatcctgtat ggcggaagtt gcacgcccga
      241 gaacgcggcc gaaatcttcg cccagcctga tgtcgacggg gcgctggtgg gcggcgcgtc
      301 gctcgacgcg gatcggttcg cggccatcgt gcgggctgcc gccgagcgcg ccaggtgacc
      361 gacggccggc cggcgcaacg tccggtggtc ctctgcgtgc tggacggctt cgggctgaac
      421 gatgatcccc ggcggaacgc gctgctggcc gcccgcatgc cggcctggcg agcactcacc
      481 gccagctggc cggcctgccg gctcggggcc tccggcgagg cggtcgggct gccggcgggc
      541 cagatgggga actcggaggt cggccacctc aacctgggcg ccggattccc ggtgctgcag
      601 gacctgcctc gcatcagcaa ggcggtggct gacggcacgt tcttcgataa cccggtgctg
      661 cgcgaagcta tcgagacggc cctgcgcagg gggagccgcg tgcacctgat gggactcatc
      721 ggtccgggcg gcatccatgc cctggacgag cacatcgagg ccatggccga gctcgcatac
      781 cgcgccgggc ttccgccgga tcgcgtcctc ctccatgcgt tcaccgatgg ccgggacacc
      841 tcaccgcgct cggccgacgt ctttctgccg gcgctcgaga gccgcctggc gggacgtgcc
      901 atcgtggcca ccgtcagcgg acgcttctac gccatggacc gcgaccgccg ctgggagcgc
      961 actgccctgg cctgggaggc gctggtgcac ggcgccggtg aacgtgccgc cggtggcacg
     1021 caggccatcg ccgcggccta cgccaggggc gagggggacg agttcatccg gccgaccgtg
     1081 gtcgacacct acgacggcat gcgcgacagc gacgtcgccg tgcacctcaa cttccgggcc
     1141 gatcgagcgc ggcagctgac tcgggcgctt gccctggacg gcttcgatgg tttcgaccgc
     1201 ggccggcggc cggccgagct gtacgtcgcg accctgaccg agtaccaggc gcgcgacgag
     1261 ctgccggtgc cggtggcctt cccgccgctg gagatcgact ccctggccgc gtacctgtcg
     1321 cgcctgggca ggcgtcagct gcaccttgcg gagacggaga agtacgccca cgtcacctac
     1381 ttcttcaacg ggggcgtgga ggcgcagctg cccggggagg agcgccggct gatcccctcg
     1441 cggcgggacg tcccgaccta cgacctggcc ccggagatga gcgcgccggg tatcaccgac
     1501 gccctgctcg aggcgatcgg atccggtacc tacgacttca tcatcgtgaa ctacgccaac
     1561 cctgacatgg tcggccacac cggcgtgtgg gacgccgcgg tgcaagccgc cgaggtggtg
     1621 gacggctgcc tggatcgcgt gagctcagcg gtcctggccg ccggcggcgc gctggtgatc
     1681 accgccgacc acggcaacat cgaggagatg cgcgacgagg cgggggcgcc ccagaccaag
     1741 cacaccacct cgccggtgcc gctgctgctg gtggccgagg cgtggcgcgc agcccgcctg
     1801 cgcgacggca tcctggccga cgtggcgccc accctctgcg agctgatggg catcgaggtg
     1861 ccgccacaga tgagtggcag gtcgctgatc ctgcgctgac ctcggcgccg ccgggcggcg
     1921 tcggcatcgg tctgataaac tgcgccccct catgaccccc ctcgtcgtcg cacagatgat
     1981 ccttgccgtg gcgctcatcg cgtccatcct gctgcagcag cgcgggacgg gcctgggtgg
     2041 cgccttcggc ggtgaggtga ccgcctaccg cagccggcgc ggaatcgagc gcacgctctt
     2101 ccgcctgacg atcgtgctgg cggtcctgtt cgtgctcttc agcctgctca acctggtcga
     2161 cttcggcgcc ctgtagggcg tgatatcatc gggttgatcg ccggtcttcg tgccggtgat
     2221 cgtcgtgccg aggtggcgga ataggcagac gcgctaggtt caggacctag tgaccgcaag
     2281 gtcgtgtggg ttcaagtccc tccctcggta ccaccctcag gcccaggtgg cggaattggt
     2341 atacgcgtac gtttgagggg cgtatgggga aacccatccg ggttcaagtc ccggcctggg
     2401 caccaccacc ttctcccggt tcgtgccttg tgctgggcaa actgccggcg gcgcccgact
     2461 caatcccgcc acccgtacgc acctgaggcg tgagaggtcc gatggggacg gcttctgtgc
     2521 ggattctccg acggctcacg tcgtgggcgt gtggtccgac ccccagatca ggctccatcc
     2581 gtcgcctcac ccgccggacg cgtgagggcg cgtgctccat cggcccgtgc aacccctcgc
     2641 tcccagcagc gttgccggga ggcaccacaa cacatgccgt aaccggtgac ggcccggtac
     2701 ttctctccta tcctgcaccc agtactttca gtgcaccgcg ccgacgtgcc ttcggctata
     2761 gcccttagga gggaacacca ttcatggtcg ccatcgctgg ttcgcagcgt cgcgtgggaa
     2821 tcgcgaccgt gctcctgctc cttggcctcg tcctttcgat gtttgcaacc ggccgcccca
     2881 tcgtcaacgt gagtcaggcg tccgcgcatg cgccgctcac cgctgactgg tcctgtaacg
     2941 gcacgaggta cttcaacgac catcggtcgg ttggtcagac gatcacttcc ggccagacca
     3001 tcgacggcgg caccatcgtc atcgagggcg acagcttcat ggagatgcgg gacaatcccg
     3061 ccggcggctc cgatccgatg catgggcaca tcgtggccga gttcgaccgc cccggcgtga
     3121 cggggaacga caccgccacg atcaccttcg accgcaacat tcagatcact tcgatctacc
     3181 tctgggacaa cgatccggcc gccggcgaga ccggctggtc gttccagggc agcctgctga
     3241 cccagaccgg gcaccagaac gccctgatca ttccgtggaa cgagaccacg aataccgtga
     3301 cgttctcggc cggcctggac tcgggcggca tcgacttctg ctacgtcgag gtgccgaacg
     3361 gcggcgggac cgagggctgc acccccggat actggaagca ggcgcaccac ttcgactcct
     3421 gggtgaccta cgttccgacc cagacgctcg agtcggtgtt cgacgtcccg gacagcctgg
     3481 gcatggacaa cttcacgctg ctccaggccc tgaacttcgg cggtggtggt ggagtcgagg
     3541 gcatggcccg caacctcctg cgccatgccg tggcctcgct gctcaacggc tcgaacccga
     3601 acgttgactc accgctgacg gacgccgagg tgatcagcgc caccaacacg gcgctggcca
     3661 gcctgaaccg cggcaccatg gagacgcaga agaacatctt cgaggacaac aacgagctcg
     3721 gctgcccgct caactgagct gacagcgcca agcgccacga ggaggctcgg cccgcagggt
     3781 cgggcctcct cccacgttcg gcgctgctct ggtcggcgtg gtagcctcga tcggcggggc
     3841 ggttagctca gctggttaga gcgtctcgtt tacaccgaga aggtcggggg ttcgagcccc
     3901 tcaccgccca ccaaaccccc tgctagcatt gatgctgtcc accccgaacc gagggcacgc
     3961 gagccatgga ccttccctgg atcattttgc tggtcgtcat tgcggccgtg gtcctgttcc
     4021 tgatcgtcat ctacaacacc ctggtccgcc tgcggaacat ggtcgacgag gcgtggaacc
     4081 agatcagcgt gcagttgaag cgacgacacg atctcatccc gaacctggtc aacgccgtca
     4141 agggctacat ggacttcgag caggagacct tgactcgagt catcgaggcc cggaaccagg
     4201 cggtcagcgc ccaggaggcc ggaccgcctg gttccgggcc tcgatgactc gagtcagggt
     4261 ctcctgctcg aagtccatgt agcccttgac ggcgttgacc aggttcggga tgagatcgtg
     4321 tcgtcgcttc aactgcacgc tgatctgg
//
LOCUS       DATACA010000193         5829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_587679, whole genome shotgun sequence.
ACCESSION   DATACA010000193 DATACA010000000
VERSION     DATACA010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587679"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VHK28_03950"
     CDS             complement(<1..631)
                     /locus_tag="VHK28_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141416.1"
                     /translation="MAPIPTPPDPLSGRLTIGFKTTPSDVTWPTLDETWDAAGQEPAF
                     SAGWMADHLTDASRERGGQAWEAFSLMAALAHRVPGKWLGQAVLANTFRHPAVLAKQA
                     TVMDHVTGGRFIVGLGAGWHEGEHQAFGIPLPLIGERISRLESAVGVLRALLSIEARA
                     EPGVTRDDAFYPLRGAVNDPPPLQREGPPIWLGGQKPRGIRLAAAAASGW"
     gene            complement(632..1837)
                     /locus_tag="VHK28_03955"
     CDS             complement(632..1837)
                     /locus_tag="VHK28_03955"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX2141417.1"
                     /translation="MTDPASDQIETRVVHAGAEPDPLTGAVVPAIQMSTTFKQDGVGG
                     LRGGFEYGRSDNPTRRALEAAIAQLEGGTHGLAYASGLAATQNILYLTQPGDRLVMSD
                     DVYGGTWRLAEKVWRRYGIRTDAVDLTDLDAVAAAIAGSADGNGTDGPARLVWIETPT
                     NPLLKVVDISAVARLAAAHGAMTVVDSTFATPFLQRPLELGADLVLHSATKYLGGHSD
                     VMGGLVATRNDELAERLRFHQNAAGAVPSPFDCFLILRGLRTLAVRMERACANARAIA
                     YWLNGRPEVAVVHWPGFHDGPHYELVERQMRMPGAMVSFELATDDLATARQFVGSTRL
                     FTLAESLGAVESLIELPAAMTHASVADSPLAVPPGLVRLSVGIEAAEDLIADLEAAFA
                     MAAVPTGAA"
     gene            complement(1841..3382)
                     /locus_tag="VHK28_03960"
     CDS             complement(1841..3382)
                     /locus_tag="VHK28_03960"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2141418.1"
                     /translation="MTQVAEPRAGAVEAARERVNHWIGGKRVEGASGRSGPVYNPATG
                     EVARQVDFASVEEVDAAVAAARQAFPAWRSTSISRRTEIMFRIRNLVDQHREEIAAHL
                     TREHGKVRTDALGEVARGLENIEFACGIPNLLKGGFSEQASTGVDVYQIRQPLGVVAG
                     ITPFNFPAMVPMWMFANAIACGNTFVLKPSEKDPSASVYLAELLKEAGVPDGVFNVVH
                     GDKVAVDRILEHPDIAAVSFVGSTPIARYIYETGTRNGKRVQALGGAKNHMVVLPDAD
                     IEMAADAAVSAAYGSAGERCMAISVVVAVGDVAEPLVEAIEKRLPKIKVGPGHEEGVE
                     MGPLITREHRDKVASYLEGGKHQGATLRVDGRQHVLYDADGGGFFLGVSLLDNVTPDM
                     ECYRNEIFGPVLEIIRVPTYTDAARIIDENPYANGTAIFTRDGGAARQFQFDVNVGMV
                     GINVPIPVPVAYYSFGGWKASLFGDLHMYGPEGIQFYTRAKIVTARWPDPATSSVDLG
                     FPQTR"
     gene            3788..>5829
                     /locus_tag="VHK28_03965"
     CDS             3788..>5829
                     /locus_tag="VHK28_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141419.1"
                     /translation="MADATLVERDAPLDELRTYLADARAGSGRFVLVRGETGIGKTAL
                     VEAFVDSVRDEIPVLAGICDGVSTPKPFGPLHDLGDRLGSELIALLDEHAGRHAVSAW
                     LIDRLSASGQAVLVIEDLHWADEATTELLTFLARRMDGMALLVVATIRDDDPATPPVR
                     RLLGQLAGMTAVRHLVLQPLSPDGVAGMAAGTQTDPDDLYRLTSGNPFFVSQVLAAGN
                     HRVPVSIGDALRARLDRLSARARSALEAAAVIGSRSEPWLLAAVAGEDVLGADECVAA
                     GLLGTPTDVITFRHELTRLAVLDELPVFRAIGLHRRTLDALQRSGSGDDARLAHHAEG
                     AGHGEEVLRHAVAAADTAMRVGANHEGVAQLRRAARFLDNADDPLRAQVLERLASALY
                     SVNRLPEAFEVRQQALEVRVRLGDTLRIAENELALGKLTFWRGEGAAGRERINRAREM
                     LEPLGDSAQLAMAYASVGGLEVGRETTLARDWTQRALEMGRRLEDPEVIAASLNTLGS
                     MAMMEGDMSGEELLLESLRVSKEHSQFERAHAALFNLVILFAQNREPARASEYLAELI
                     DYVSGVQIERCNLDCTLARLQLDMGSWTEAERSAQLALEYARTPSDDQGLALTTLATL
                     AIRRGAEGWRALLDRAEQIVAGFDDFEFLVPVIRARAEAAWLDGTLSSLQPRLREGV"
BASE COUNT          867 a         1999 c         2066 g          897 t
ORIGIN      
        1 gccagccgct ggccgcggcc gccgccagcc gaataccgcg cggcttctgg ccgcccaacc
       61 agatgggcgg tccttcgcgc tgcaacggcg gcgggtcatt gacggcgccg cgcagcgggt
      121 agaacgcgtc gtctcgggtg acgcctggct cggcgcgcgc ctcgatcgat aagagggctc
      181 gcagcacgcc aaccgccgac tccaggcggc tgatgcgctc gccgatgagc ggcagcggga
      241 tgccgaatgc ctggtgctca ccctcgtgcc acccggcgcc cagaccgacg atgaatcgtc
      301 cgccagtgac gtggtccatg acggtggcct gcttggccag gaccgccgga tgacggaacg
      361 tgttggccag caccgcctgc cccagccact tgccgggcac tcgatgggcg agcgcggcca
      421 tgaggctgaa tgcctcccag gcctgtccgc cgcgctcgcg ggaggcatcg gtcaggtgat
      481 ctgccatcca gccggcgctg aacgcgggct cctgcccggc ggcgtcccag gtctcgtcca
      541 gggtcggcca ggtgacgtcg ctgggcgttg tcttgaagcc gatggtcagc cttccgctga
      601 gcggatccgg cggcgtggga atgggcgcca tctaggccgc gccggtgggg accgccgcca
      661 tggcgaaggc cgcctccagg tcggcgatca ggtcctcagc ggcctcgatc ccgaccgaga
      721 ggcgcacgag acccggcggc acggccaatg gcgaatcggc cacggacgcg tgggtcatgg
      781 ccgcgggcag ctcgatcagc gattccaccg cacccagaga ctcggccagg gtgaacaggc
      841 gggtggagcc gacgaactgg cgagccgtgg ccaggtcatc ggtcgccagc tcgaagctga
      901 ccatggcccc cggcattcgc atctggcgct cgaccagctc gtagtgcggg ccgtcgtgga
      961 agccgggcca gtggacgacg gccacctccg gccgcccatt cagccagtag gcgatggcac
     1021 gcgcgttggc gcaggcacgc tccatgcgca cggccagcgt ccgcaggccg cgcaggatga
     1081 ggaagcagtc gaacggcgag ggaacagcgc cggcagcatt ctggtggaag cgcaggcgct
     1141 cggccagctc gtcgttgcgg gtggccacca ggccgcccat cacgtcggag tggccgccca
     1201 ggtacttcgt ggccgagtgc agaaccaggt cggcccccag ctcgagggga cgctgcagga
     1261 agggcgtggc gaacgtcgag tcgaccacgg tcatcgcccc gtgcgccgcg gccaggcggg
     1321 cgaccgcgga gatgtcgacc actttgagca gcggattggt gggcgtctcg atccacacca
     1381 ggcgcgccgg cccatcggtc ccgttgccgt cagcggagcc ggcgatggct gctgccaccg
     1441 cgtcgaggtc ggtgaggtca accgcatcgg tcctgatccc gtagcgacgc cagacctttt
     1501 cggccaggcg ccaggtgccg ccgtagacgt cgtccgacat caccagccga tcgccgggct
     1561 gggtcaggta caggatgttc tgggtggccg ccagcccgga ggcgtaggca aggccgtggg
     1621 tgcccccctc cagttgcgcg atcgcggcct ccagggcgcg gcgggtcgga ttgtccgagc
     1681 ggccgtactc gaatccgccg cgcagccccc cgacaccgtc ctgcttgaag gtggtgctca
     1741 tctggatcgc gggaacgacg gcgccggtca gcggatcggg ctcggcgccg gcgtggacga
     1801 cccgggtctc gatctggtcg ctggcgggat cggtcattcg tcagcgagtc tgcgggaagc
     1861 ccaggtccac ggacgaggtg gccggatccg gccagcgtgc cgtcacgatc ttggcgcgag
     1921 tgtagaactg gatgccctcg ggaccgtaca tgtgcaggtc gccgaagagg gacgccttcc
     1981 agccgccgaa gctgtagtag gccaccggca ccgggatcgg gacgttgatg ccgaccatgc
     2041 cgacgttgac gtcgaactgg aactggcggg ccgccccgcc gtcgcgggtg aagatcgccg
     2101 tgccgttggc gtacgggttc tcgtcgatga tgcgagcagc atcggtgtag gtcggcacgc
     2161 ggatgatctc cagcaccggg ccgaagatct cgttgcggta gcactccatg tccggcgtca
     2221 cgttgtccag aagggagacg cccaggaaga agcccccacc atcggcgtcg tagaggacgt
     2281 gctgccgccc gtccacccgc agcgtcgctc cctggtgctt gccgccctcc aggtaggacg
     2341 ccaccttgtc gcggtgctcg cgagtgatga gagggcccat ctcgaccccc tcctcgtgcc
     2401 ccggccccac cttgatcttc ggcagccgct tctcgatggc ctccaccagc ggctcggcca
     2461 cgtcgccgac cgccaccacc acgctgatgg ccatgcagcg ctcaccggcc gagccgtagg
     2521 cggccgagac cgcggcgtcg gccgccatct cgatgtcggc atcgggcagg acgaccatgt
     2581 ggttcttggc gccgcccagg gcctgcaccc gctttccgtt cctggtgccg gtctcgtaga
     2641 tgtagcgcgc gatgggcgtg gagcccacga acgagacggc cgcaatgtcc ggatgctcga
     2701 ggatgcggtc gacggccacc ttgtcgccgt ggaccacgtt gaagacgccg tcgggaacgc
     2761 ccgcctcctt cagcagctcc gcaaggtaga ccgatgcgga ggggtccttc tcggacggct
     2821 tcaggacgaa cgtgttcccg caggcgatgg cgttggcgaa catccacatc ggcaccatgg
     2881 ccgggaagtt gaagggcgtg atgccggcca ccacgcccag tggctgccga atctggtaga
     2941 cgtccacgcc cgtcgacgcc tgctcgctga agccgccctt gagcaggttg gggatgccgc
     3001 atgcgaactc gatgttctcc aggccgcgcg ccacctcgcc cagcgcatcg gtgcgcacct
     3061 tgccgtgctc gcgggtgagg tgggcggcga tctcctcgcg atgctggtcg accaggttcc
     3121 gaatgcggaa catgatctcg gtgcgccggc tgatggatgt ggagcgccac gccgggaagg
     3181 cctggcgggc agcggcgacg gcggcgtcca cctcctcgac cgaggcgaag tccacctggc
     3241 gcgccacctc cccggtggcc gggttgtaga ccggaccgct gcggccgctg gcgccctcca
     3301 cccgcttgcc gccgatccag tggttgaccc gctcgcgtgc cgcctcgacg gcacccgcac
     3361 gcggctctgc gacctgcgtc atgtgctcta cctcacgttc ctcggagaga tgactttact
     3421 ccgaccggca tggaacgtca ggtaagggct tcaatcgtcc agggtctgga ccgaaacgag
     3481 ctcgcggcgt atcgtcgccc tcatggaaga ggcggctggg tgcgccatct gcggccaacc
     3541 aatcgacgtg ggccaggcct ggatggaggc tgaccgcgac ggcgcgcgtg tccgcgccca
     3601 ttccgagtgc ctgtaccggg acgaacgggc aggagagggc tgggagccac aggaccacgc
     3661 ggcgagctga cgcggcaggc gacgtcgcct atagaccgtt gccgttccgc ggcgtatggt
     3721 cggaggtatg gccctgtcaa cccgtgactc gggactgcgc gtgacgtccc agagcgatgt
     3781 ccgcgtcatg gctgacgcga cgctcgtcga gcgtgacgcc cccctcgatg agctcaggac
     3841 gtacctggcc gacgctcgcg ccgggagcgg acggttcgtc ctggtgcgtg gcgaaacggg
     3901 gatcggcaag accgcactgg tcgaagcctt cgtcgactcg gtccgtgacg agatcccggt
     3961 gctggccggc atctgcgatg gcgtcagcac gcccaagccc ttcggtcctc tgcacgacct
     4021 gggcgaccgc ctgggcagcg aactcatcgc cctgctcgac gagcatgcgg gtcggcatgc
     4081 ggtcagcgcc tggctgatcg atcggctgtc cgcttctgga caggcggtgc tcgtgatcga
     4141 ggaccttcac tgggccgacg aggcgactac cgagctcctc accttcctcg cccgccggat
     4201 ggacggcatg gcgctcctgg tggtggccac catccgcgac gacgatcccg cgaccccgcc
     4261 cgtgcgccgc ctcctggggc agctggccgg gatgacggcc gtgcgccacc tggtcctcca
     4321 gcccctgagc cctgatggag tggcgggcat ggcagccggc actcagacgg acccggatga
     4381 cctctatcgg ctgacctcgg gcaacccatt cttcgtaagc caagtgctgg ccgccggcaa
     4441 tcaccgcgtc ccggtgtcca ttggggacgc cctgcgtgct cgcctcgacc gcctgtcggc
     4501 ccgcgccagg tccgcgctcg aagcggccgc ggttatcggc tcgcgcagcg agccatggct
     4561 gctcgccgcc gtggccgggg aggacgtgct cggcgccgac gagtgcgtgg ctgcgggcct
     4621 gctggggacg ccgaccgacg tgatcacctt ccggcatgag ctgacacggc tggctgtcct
     4681 ggacgagctc cccgtcttcc gcgccatcgg cctgcaccgg cgcaccctgg atgcgttgca
     4741 gcgcagcgga tcgggcgacg acgcgcgact ggcgcaccac gccgagggcg ccgggcatgg
     4801 cgaggaagtc ctgcgccacg cggtcgcggc agccgacacc gccatgcgcg tcggtgcgaa
     4861 ccacgagggg gttgcccagc tgcggcgtgc agcccgcttc ctggacaacg cggacgaccc
     4921 gctgcgtgcg caggttctcg agcggttagc atcggcactc tattcggtca atcgcctgcc
     4981 cgaggccttc gaggtgcggc agcaggcgct cgaggtgcgc gtccgcctgg gcgacacgct
     5041 ccgcatcgcc gagaacgagc tggcgctcgg caagctcacc ttctggcgtg gcgagggtgc
     5101 tgccgggcgc gagcgtatca accgtgcacg tgagatgctc gaaccgctgg gggacagcgc
     5161 ccagctggcc atggcgtatg ccagcgtcgg ggggctcgag gtcggtcgcg agaccacgct
     5221 tgcgcgagac tggacgcagc gtgccctgga gatgggccgt cggctggaag accccgaagt
     5281 gatcgcggcg tccctcaaca cgctgggttc gatggccatg atggaggggg acatgtccgg
     5341 cgaggagctc ctgctggaga gcctgcgggt ctcgaaggag cacagccagt tcgagcgagc
     5401 gcatgccgcc ctcttcaacc tcgtcatcct tttcgcccag aatcgcgagc ctgcgcgggc
     5461 gagcgaatac ctggccgagc tgatcgacta cgtgtccggc gtgcagatcg agcgctgcaa
     5521 cctggactgc acgctggcgc ggctgcagct ggacatgggc tcctggaccg aggccgagcg
     5581 ctcggcgcag ctcgcgctcg agtacgcccg tacaccctcg gacgaccagg gattggcgct
     5641 tacgacgctg gccacgctgg ccattcgacg tggggccgag ggatggagag cgctccttga
     5701 ccgggccgag cagatagtcg cgggctttga cgacttcgag ttcctggtgc cggtcatacg
     5761 ggcgcgcgcc gaggctgcct ggctcgacgg gacgctttcg tcactgcagc cgcggctgcg
     5821 tgagggcgt
//
LOCUS       DATACA010000194         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1632056, whole genome shotgun sequence.
ACCESSION   DATACA010000194 DATACA010000000
VERSION     DATACA010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632056"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VHK28_03970"
     CDS             <1..1176
                     /locus_tag="VHK28_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2141420.1"
                     /translation="RDAALADGRSPTLQRGISILVEDGRVAWIRPSDGEEDAGDALVV
                     DASGATVVPGMVDGHSHVTSPGGAHWIERFDDATDRLLAYAEANGRLAWDAGVRWLRD
                     VGSPTREDPADGRQRALALGVRDRWNGRHGLPVIRAAGTWLTGAGVLVGRGLQAANAD
                     ELLALAMRQLDDGADLVKLYLDGPDPAVSPWSAAEVGRVVAAVHERGARLTAHAGRLD
                     GARVAAEAGVDAVEHGFELDAAVAARMAERGIMLVSTLTVYRSWITFGRTTTIPNFAG
                     AGRSAVWQAGLEAAEASVRTAAAAGVAIAAGTDFGGGSARANQLAWEVESLVAAGLQP
                     VEALAAATWRGGELLGEPDAGVIREGGPADFFLVHGDPLSDPAALWRVWRHGVPLTR"
     gene            1191..1988
                     /locus_tag="VHK28_03975"
     CDS             1191..1988
                     /locus_tag="VHK28_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2141421.1"
                     /translation="MTEQAIFRAPAAGAPVLVVRDLHKRYGSLEAVDGVSFEVRQGEI
                     FGILGPNGAGKTTTLEMIEGMRAIDSGSATVDGVDVARNPRGVKARIGIQLQASAFFD
                     ELTLVELLELFGHLYGRDVNASALLASVELTEKERSLVRTLSGGQKQRFSIAAALVNE
                     PRVLFLDEPSTGLDPQARRHLWALIRDIRERGHTVVLTTHYMEEAEELCDRVAIMDRG
                     RIVALETPQGLVDDLLARGFRKARVERLANLEDVFIDLTGHELREDG"
     gene            1995..3077
                     /locus_tag="VHK28_03980"
     CDS             1995..3077
                     /locus_tag="VHK28_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141422.1"
                     /translation="MKRRLQLFWVLFVASVKMFVRNRVALFFSLFLPMVIMLIFGVLN
                     FGGSASISMGLVDEAQTDASTALASAVEDVETLEVADGGRDALLTALEEGDLDMVVVI
                     PEGWGEEPETSQLTAYASAASQQNAQLGTLILNAIAAQVLFGGPGTPSGPLVAVEEVN
                     ARELGYIDFLVPGILGLTIMQLGVFGVAFGFVQLKRTGALRRLFATPTPPRYFLAAQI
                     ASRLVLGLAQVGILLGIGLLFGLNLVGSVPLLLAVAVLGAVIFLGIGFAIAGWAKNED
                     QAAPVANIISLPMMFLSGTFFPREAMPSFLQGVTDFMPLTYLNQALRSIANEGAGVVE
                     IGGDLLGMAVWAVISFVIAVRLFSWE"
     gene            complement(3088..3450)
                     /locus_tag="VHK28_03985"
     CDS             complement(3088..3450)
                     /locus_tag="VHK28_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2141423.1"
                     /translation="MGQVSLYIRDVPRAERFYGEILGLPHLYTFGDLAFFEAGGMRIY
                     LHRKDEADWQPGSILYFECPDIRAAHEALAARGAEFSGPPQLVHRHDDGTEEWMAFFS
                     DGQGNMLALMSRVPATTA"
     gene            complement(3490..3789)
                     /locus_tag="VHK28_03990"
     CDS             complement(3490..3789)
                     /locus_tag="VHK28_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX2141424.1"
                     /translation="MNEQQTSPGGRRTEEFNLNGDQVVAKVKELVHEGNIRRISIKNE
                     DGRTILEVPLTLGVIGAALLPVFAAIGAAAALATRCTIVVERDDNAAPPTAAGSE"
BASE COUNT          582 a         1554 c         1607 g          644 t
ORIGIN      
        1 cgggacgcgg cgctggccga cggccgctcg ccgacgctgc agcgcgggat cagcatcctg
       61 gtggaggacg gccgtgtggc gtggatccga ccctccgacg gcgaggagga cgccggcgac
      121 gcactggtcg tggatgcctc cggcgccacc gtggtccccg gcatggtgga cggccactcg
      181 cacgtcacaa gcccgggcgg cgctcactgg atcgagcgct tcgacgacgc gaccgaccgg
      241 ctgctggcgt acgcggaggc caacggtcgc ctggcgtggg acgcgggcgt gcggtggctg
      301 cgggacgtcg gctcgccgac gcgcgaggac ccggctgacg ggcgccagcg ggcgctggcg
      361 ctcggcgtgc gggaccgatg gaacggccgc cacggccttc ccgtcatccg ggcggccggc
      421 acctggctga ccggtgccgg cgtcctggtc gggcgcgggc tgcaggcggc caacgcggac
      481 gagctgctgg cgctggccat gcgacagctc gacgacggcg cggacctcgt caagctgtac
      541 ctggacgggc cggatccggc cgtctccccc tggtcggcgg ccgaggtggg ccgggtggtg
      601 gccgcggttc acgaacgggg cgcgcggctg acggcgcacg ccggacggct ggacggggcg
      661 cgggtggcgg ccgaggcggg agtggacgcc gtcgagcacg gcttcgagct ggacgcggcg
      721 gtggccgcgc gcatggcgga gcgggggatc atgctggtct cgaccctgac cgtctaccgc
      781 tcgtggatca ccttcgggcg gacgaccacc atccccaact tcgccggcgc cgggcgctcg
      841 gcggtatggc aggcggggct ggaggcggcc gaggccagcg ttcggacggc tgccgccgcg
      901 ggcgtggcca tcgcggccgg gaccgacttc ggcggtggct ctgcccgcgc caaccagctg
      961 gcctgggagg tggagtcgct ggtggcggct gggctccagc cggtcgaggc gctggctgcg
     1021 gccacctggc gcggcggcga gctgcttggc gagccggacg ccggcgtcat ccgcgagggc
     1081 ggacccgccg acttcttcct ggtgcacggc gacccgctct ccgacccggc ggcgttgtgg
     1141 cgcgtctggc gtcacggggt gccgctgacc cgctagcatt cggtcaccct atgaccgagc
     1201 aagccatctt ccgcgcgccg gccgccggtg ccccggtcct ggtcgtccgc gacctgcaca
     1261 agcgctacgg cagcctcgag gcggtcgacg gcgtcagctt cgaggtgcgc cagggggaga
     1321 tcttcggcat cctcggccca aacggcgcgg gcaagaccac cacccttgag atgatcgagg
     1381 gcatgcgggc catcgactcc gggagcgcga ccgtcgacgg cgtggacgtc gcccgcaacc
     1441 cacggggcgt gaaggcgcgc atcggcatcc agctccaggc ttcggccttc ttcgacgagc
     1501 tgaccctggt cgagctgctc gagctcttcg gccacctcta cgggcgggac gtcaatgcct
     1561 ccgccctcct cgccagcgtg gagctcaccg agaaggagcg ctcgctggta cggaccctgt
     1621 ccggcggaca gaagcagcgg ttcagcatcg ccgccgcgct ggtgaacgag ccgcgggtcc
     1681 tgttcctgga cgagccgagc accggcctcg acccgcaggc gcgccgtcac ctgtgggcgc
     1741 tgatccgcga cattcgcgag cgtggacaca cggtggtgct gaccacgcac tacatggagg
     1801 aggcggagga gctctgtgac cgggtggcca tcatggaccg tggccgcatc gtggcgcttg
     1861 agaccccgca ggggctggtc gacgacctcc tggcgcgcgg cttccgcaag gcgcgcgtgg
     1921 agcgcctggc caacctggag gacgtcttca tcgacctcac cggccacgag ctgcgcgagg
     1981 atggctgagg accgatgaaa cggcggctcc agctgttctg ggtcctcttc gtggcgagcg
     2041 tcaagatgtt cgtgcgcaac cgggtggcgc tcttcttcag cctgttcctg ccgatggtga
     2101 tcatgctcat cttcggcgtc ctcaacttcg gcggcagcgc ctcgatctcg atgggcctgg
     2161 tcgacgaggc acagaccgat gccagcacgg cgctggcctc agcggtggag gacgtggaga
     2221 ccctggaggt cgccgacggc ggacgcgacg ccctgctgac ggccctcgag gagggggacc
     2281 tggacatggt cgtcgtcatc cccgagggct ggggtgagga gcccgagaca tcccagctga
     2341 ccgcctacgc ctcggccgcc agccagcaga acgcgcagct cggcacgctg atcctgaacg
     2401 ccatcgccgc ccaggtgctg ttcggcgggc cggggacgcc cagcggcccg ctggtggcgg
     2461 tcgaggaggt caacgccagg gagctgggct acatcgactt cctggtgccg ggcatcctgg
     2521 gcctgaccat catgcagctg ggcgtcttcg gcgtggcctt cggcttcgtg cagctcaagc
     2581 gcaccggtgc gctgcgccgg ctgttcgcca cgccgacccc gccgcgctac ttcctggcgg
     2641 cgcagatcgc ctcgcggctg gtgctgggcc tggcccaggt ggggatcctg ctgggcatcg
     2701 gcctcctctt cgggctcaac ctggtgggca gcgtgccgct gctgctggcg gtcgcggtgc
     2761 tgggtgcggt catcttcctg ggcatcggct tcgcgatagc cggttgggcc aagaacgagg
     2821 accaggcggc gcccgtggcc aacatcatca gcctgcccat gatgttcctg tcggggacat
     2881 tcttcccgcg cgaggcgatg ccctccttcc tccagggcgt gaccgacttc atgcccctca
     2941 cctacctgaa tcaggcgctg cgcagcatcg ccaacgaggg tgccggcgtg gtcgagatcg
     3001 gcggcgacct gctgggaatg gccgtctggg cggtgatcag cttcgtgatc gccgtccgcc
     3061 tcttctcctg ggaatagcgc gcttggccta ggctgtggtc gctgggaccc gggacatcaa
     3121 ggccagcatg ttcccctgcc cgtcggagaa gaaggccatc cactcctcgg tgccgtcgtc
     3181 gtggcggtgg acgagctgcg gcggaccgct gaactccgcc ccgcgcgcag ccagcgcctc
     3241 gtgcgctgcc cggatgtcgg ggcactcgaa gtagaggatg gatcccggct gccagtccgc
     3301 ctcgtccttt cggtgcaggt agatgcgcat gccgcccgcc tcgaagaagg ccagatcgcc
     3361 gaaggtgtac aggtgtggca gcccaaggat ctcgccgtag aagcgctccg cgcgcgggac
     3421 gtcgcggatg tacagggata cctgacccac cggacccagc cggccggcat ctgctccgtc
     3481 caccccacgc tactccgagc cggctgccgt tgggggcgcg gcgttgtcgt cgcgctcgac
     3541 cacgatggtg cagcgcgtgg ccagcgccgc cgccgccccg atggccgcga acaccggcag
     3601 cagcgccgcc ccgatcacgc ccagggtcag cggcacctcc aggatcgtcc gcccgtcctc
     3661 gttcttgatg ctgatgcgcc ggatgttgcc ctcgtggacg agctccttga ccttggccac
     3721 cacctggtcg ccgttcaggt tgaactcctc ggtgcgccgc ccgcccgggc tcgtctgctg
     3781 ttcgttcatg agtggagcct actccgcatg cagcctgcca tcggggtcga ccatccagga
     3841 ccgatgcctc ccggtcacct cgcgcggggc cacgaccggc tgacaccacg tcgccgcccg
     3901 cgctcagcac gaacgaccgg tggcctgccc ggcgcagcgc gccggccgcc cagcgcagag
     3961 ccagcccctt gccgatgccg cccaggtcca ccggcgccag cagccgcgcg cggccgcgtc
     4021 gggggtcgag cgacagccag cgttcacccg gcccgagccg cggcggcgat ggcggcaggc
     4081 gcacgccggc ccgctcgccg gcaacctcca gcgcaccgat gatcctggga tcgaagcggc
     4141 cgctgctcac ccggtaggca cgccaggcga cggccagggc gcgcgccagg aggggcgaca
     4201 ccggctgcca gcggccgacc gcgccgttca gccggctgag gggactgcat gcgtcaaagc
     4261 gggtgagctc cgcctcggcc cgggcgaagg tgccggccac gacgctgaag gcggcatcgc
     4321 ccagtggggc atcgtggacg ccgggcagtg tcaggcggac tgcggagccc atcgcgcgtc
     4381 cggtgcg
//
LOCUS       DATACA010000195         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_26218, whole genome shotgun sequence.
ACCESSION   DATACA010000195 DATACA010000000
VERSION     DATACA010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26218"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VHK28_03995"
     CDS             <1..690
                     /locus_tag="VHK28_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX2141425.1"
                     /translation="PIAESDRTEPINPYGATKLAFEGAMRWFAAAHGFQAISLRYFNV
                     AGATERNGEDHDPETHLIPLVLRVAAGEAGHVQIFGQDYPTPDGTCIRDFIHVRDLAA
                     AHLLALEATGDADPSLEVYNLGSAAGFSVREVVEAARKVTGRAIPARVVKRRLGDPPV
                     LVASSRRARRELGWQPQHSKLEQMLSDAWSWKMAHPQGYPHGANGQAAQQSGPSRDER
                     VTAGAPASAST"
     gene            complement(682..1128)
                     /locus_tag="VHK28_04000"
     CDS             complement(682..1128)
                     /locus_tag="VHK28_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2141426.1"
                     /translation="MASPQGAAPVPERLRAAGERVTPRRLSVAAALAGAARQQTADEL
                     WRRLRRSDPGIGRATIFRTLETLVGAGVARRLELENHVYGYVACEPEHHHHLVCTACG
                     RVEEIGEGYVRPVAGQVAADTGFVIDDARLDFYGRCAGCARADYSG"
     gene            complement(1128..1877)
                     /locus_tag="VHK28_04005"
     CDS             complement(1128..1877)
                     /locus_tag="VHK28_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX2141427.1"
                     /translation="MPLTAVLVSMAAFASAIAGGVLALRAVRSVGLIIAVGAGIRIGA
                     AFFDLMPEAVEHLGSLDAAMLFTAVGFLAFYAVEKLTTLHVGHEAATEMDHGEAAHRH
                     VGVIGASGMSLHSFLDGVALAAGLVVGGGLGLVIAAVVIIHRFSDGIAIVSFLLASRT
                     PLRETYRWVMVVALAPVAGVLLGLLIPIPDEVLGGMLAVFAGFFLYIGAAELLPEAHR
                     SNRSRWLVGATMAGVLAIYLFSMFAGAVGAH"
     gene            1985..>2887
                     /locus_tag="VHK28_04010"
     CDS             1985..>2887
                     /locus_tag="VHK28_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2141428.1"
                     /translation="MPETDTRPARRRASTTRRAAPEPAADATPQDDTAESPLLAAVLA
                     AQRTYRFGEIELPPTIARAIEKMGFTQPTEVQARAIGPLRAGKDLIGQAQTGTGKTAA
                     FGIPIVEKIDPGANHVQALVLTPTRELCVQVTEELRRIGTFRNVGAVAIYGGSSMERQ
                     ISALKRGVPIVVGTPGRVLDLIRRGELRLDRVHTVILDEADRMLDMGFIVDVETILAR
                     CPRERQTALFSATMPYAILRICDRFMKREPEHITVRPEMRTVETVRQLVYFVAEQDKA
                     KALLELTRHFGFDRLLVFRHTQIGV"
BASE COUNT          461 a         1073 c          986 g          367 t
ORIGIN      
        1 cccatcgccg aatcggacag gaccgagccc atcaacccct acggcgccac caagctcgcc
       61 ttcgagggcg ccatgcgctg gttcgccgcg gcgcacggct tccaagccat cagcctgcgc
      121 tacttcaacg tggccggcgc caccgagcgc aacggggagg atcacgaccc ggagacgcac
      181 ctcatcccgc tggtcctgcg cgtggcggct ggtgaggcgg gccacgtcca gatcttcggg
      241 caggactacc cgaccccgga tggaacctgc atccgcgact tcatccacgt ccgcgacctg
      301 gccgccgcgc acctgctggc gctggaggcg accggcgacg ccgatccctc gctggaggtg
      361 tacaacctcg gctcggccgc cggcttcagc gtccgcgagg tggtggaggc ggcgcgcaag
      421 gtgaccggtc gcgccatccc ggcccgcgtc gtgaagcgtc ggttggggga tccaccggtg
      481 ctggtggcct cctctcgtcg cgcccgtcgg gagctcggct ggcagccgca gcacagcaag
      541 ctggagcaga tgctgtcgga tgcctggtcc tggaagatgg cccatccgca gggctatccg
      601 cacggggcga acggtcaggc ggcgcagcag tccggaccga gccgggacga gcgtgtgacc
      661 gccggcgccc cagcctcggc atcaacctga gtagtcagcc cgggcgcagc cggcgcagcg
      721 cccgtagaag tcgagccgcg cgtcgtcgat gacgaagccg gtatcggcgg ccacctggcc
      781 ggccaccggc cgcacgtagc cctcgccgat ctcctccacc cgcccgcagg cggtgcacac
      841 caggtggtga tggtgctccg gttcgcaggc cacgtagccg tagacgtggt tctccagctc
      901 cagccggcgc gccacgccgg cacccaccag cgtctccagg gtgcgaaaga tggtggcacg
      961 gccgatgccc ggatcgctgc ggcgcagccg ccgccacagc tcgtcggcgg tctgctgccg
     1021 ggcggcgccg gccagcgcgg cggccaccga gaggcggcgg ggggtcaccc gctccccggc
     1081 cgcccgcagc cgctcgggga ccggggccgc accttgggga ctggccatca gtgcgctccc
     1141 accgccccgg cgaacatcga gaacaggtag atggccagca ccccggccat ggtggcaccc
     1201 accagccagc gcgagcggtt ggaccgatgc gcctccggca gcagctcggc ggctccgatg
     1261 tacaggaaga acccggcgaa gacggccagc atgccgccca gcacctcgtc ggggatgggg
     1321 atcagcagcc ccagcaacac gcccgcgacc ggggccaggg ccaccaccat cacccagcgg
     1381 tacgtctcgc gcagcggggt gcggctggcg agcaggaagc tgacgatggc gatcccgtcg
     1441 ctgaagcggt ggattatgac caccgccgcg atcaccaggc ccagcccgcc gcccaccacc
     1501 aggccggcgg ccagcgccac gccgtccagg aacgagtgca ggctcatgcc gctggcaccg
     1561 atgaccccca cgtgccggtg ggcagcctcg ccatggtcca tctcggtggc cgcctcatgg
     1621 ccgacgtgca gggtggtcag cttctcgacc gcgtagaagg ccaggaagcc gacggcggtg
     1681 aacagcatgg cggcgtccag cgaaccaagg tgctcgaccg cctcgggcat caggtcgaag
     1741 aaggcggcac cgatgcggat gccggcgccc acggcgatga tcagccccac cgagcgcacc
     1801 gcccggagcg ccagcacgcc gccggcgatc gccgaggcga atgcggccat cgagaccagc
     1861 accgcggtca gcggcatcgg tcccagcccc ggcttggtac gcgtcgtctc tccatgtgag
     1921 acgcagtatc atctacgtgt caagccggcc gcgtggtacg atcgcggtat tacaggagcc
     1981 ttgtttgcca gagactgaca cccgcccggc gcgtcgccgg gccagcacta cccgccgcgc
     2041 cgcccccgag ccggcggccg atgccactcc ccaggacgac accgccgagt ccccgctgct
     2101 cgccgcggta ttggccgccc agcggacgta tcggttcggc gagatcgagc tgccgcccac
     2161 catcgcgcgc gccatcgaga agatgggctt cacgcagccg accgaggtcc aggcgcgggc
     2221 catcggtccg ttgcgcgccg ggaaggacct catcggtcag gcccagacgg gcaccggcaa
     2281 gaccgcggcc ttcggcatcc ccatcgtcga gaagatcgac ccgggtgcca accacgtcca
     2341 ggcgctggtc ctcacgccca cccgcgagct gtgcgtgcag gtgaccgagg agctgcgccg
     2401 catcggcacc ttccgaaacg tcggtgcggt ggccatctac ggcggctcga gcatggagcg
     2461 ccagatcagc gcgctgaagc gcggggtgcc gatcgtggtc ggcacgccgg gccgcgtact
     2521 ggacctcatc cgccgcgggg agctgcgcct ggaccgggtg cacaccgtca tcctggacga
     2581 ggcggaccgc atgctggaca tgggcttcat cgtggacgtc gagaccatcc tggcccgatg
     2641 cccgcgcgag cggcagacgg ccctcttcag cgccaccatg ccgtacgcca tcctgcgcat
     2701 ctgcgaccgc ttcatgaagc gcgagccgga gcacatcacg gtgcgccccg agatgcgcac
     2761 cgtggagacc gtccggcagc tggtgtactt cgtcgccgag caggacaagg ccaaggccct
     2821 gctggagctg acgcgccact tcggcttcga ccgcctgctg gtcttccgcc acacgcagat
     2881 cggcgtg
//
LOCUS       DATACA010000196         4540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_910395, whole genome shotgun sequence.
ACCESSION   DATACA010000196 DATACA010000000
VERSION     DATACA010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910395"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VHK28_04015"
     CDS             <1..641
                     /locus_tag="VHK28_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX2141429.1"
                     /translation="RDGAEIIFTGPSSEERRVVARQVVEERGHVMIEPYDAPAIIAGQ
                     GTAGLEIAEDLPQVTSVLIPVSGGGLSAGIATAVKSLAPSARIVGVEPELAADARESL
                     AAGEIVQWDASLTTRTAADGLRVEHLGWLPFVHLRRYIDEIVTVSEEAMTDAIRRLAS
                     GARLVVEPSGAAAMAAHLTGAAPQPDGDDHRVIVISGGNIDPKSLAEILASG"
     gene            complement(645..1754)
                     /locus_tag="VHK28_04020"
     CDS             complement(645..1754)
                     /locus_tag="VHK28_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX2141430.1"
                     /translation="MADRDSRIPVFRTDDHLGHSGLVEVNAGREVTCWESPQRAVEIE
                     RALLAAGGFRLEEPREFGREPILAVHDPELLEVVDRAWTDAVAAGADESRPLIPDTFR
                     LDAYAGPMLRLPLPDAAHLRLGAFCFDTATPIVAGTAAAAHAAVDVALSAAAAVAHGD
                     AALAYGLCRPPGHHAARGMLGGYCYFNNAAIVAEWLRREAGARRVAIVDVDYHHGNGT
                     QQIFWERPDVLYASLHGDPARAYPYFSGSSAERGGGPGEGSTLNFPLPAGTTLDAYAT
                     ALAAALDRAADFDPDGPLVVSIGFDTFERDPISDLALRTADYAEVGRMIGSTASGGSA
                     GSVIAVQEGGYAVDAIGANAVAFLAGLRQGLDGSA"
     gene            1801..2520
                     /locus_tag="VHK28_04025"
     CDS             1801..2520
                     /locus_tag="VHK28_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX2141431.1"
                     /translation="MSTLDDQAALEALRGCPLFAPCPEPVLAEVGRRLRHRHFRRNEV
                     IFHQGDPGDALHVITSGSVKIVLPSPEGEEAIVATLRPGDFFGELALLDGKPRSASAV
                     ALEQTETLSLPRDVFRELLNQHPELCDALFASLATLLRRITRHVEELHFLDLAGRLAA
                     RLAELARQAQPRGAGLGPEAGPIELDWPYTQSDLASMIGGTRQSVNRLLAELMDDGLV
                     RLEHERLIVNDLRALERRAER"
     gene            complement(2542..3030)
                     /locus_tag="VHK28_04030"
     CDS             complement(2542..3030)
                     /locus_tag="VHK28_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2141432.1"
                     /translation="MAAAVTRLNHAVLFVRDVDRASAFYRDAFGFEEIERPAGMRAVF
                     MRSPAGDNHHDLGLFEVGQAAARPPRGSVGLYHLAWEVESIEGLAEMAGRLSEMGAMG
                     GASDHGVSKSLYGRDPDGNEFEVMWRVPREAWGEYAEQAVVKPLDLDAELERWGRGAA
                     VH"
     gene            complement(3032..3757)
                     /locus_tag="VHK28_04035"
     CDS             complement(3032..3757)
                     /locus_tag="VHK28_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2141433.1"
                     /translation="MVAAAPRLQPSILPLRALPEREIVAQAISYPKELVTMRVAVIGI
                     GNVGSTLSEKLESAGHEVVVAASTEESAERAAEDLNCEPAATPAEAAEKAEAVVLAVP
                     ADALGEVAQELRGHVSGKPVVDVSNGPMAVQEGRSLAETVQEQLPEARVVKAFNTAFA
                     SRMADPTFDGGLSLDGFLAGDDPEAKKVVGRLVGDVGFEPLDIGELKMARVLEGMAWV
                     NISLNMANSWPWRSGWKLLRQEA"
     gene            3854..>4540
                     /locus_tag="VHK28_04040"
     CDS             3854..>4540
                     /locus_tag="VHK28_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX2141434.1"
                     /translation="MFYVKRGEVPHKRHTQFRKGDGSLYAEELFGVEGFAGRSSLLYH
                     HTPPTQTHQIDDPSSVRLEPADPEGGSPHRHHLVNTAQLAASGDGLSGRIPLFYNSDV
                     TFGVVLPEGPMTDFYRNGVADEMYFVHSGSGTLETNFGPIPYGPGDYLVIPIGTTYRL
                     VPDGGVAQRMLWLECPSEIEPPKRYRNEYGQLLEHSPYYHRDIRPPDEMPPVTDADGD
                     HVILVRSRDRI"
BASE COUNT          659 a         1688 c         1565 g          628 t
ORIGIN      
        1 ggcgggacgg ggccgagatc atcttcaccg ggccgtcgtc ggaggagcgg cgcgtggtcg
       61 ccaggcaggt cgtcgaggag cggggccacg tgatgatcga gccctacgat gcgccggcca
      121 tcatcgccgg ccaggggacc gccggcctgg agatcgccga ggaccttccg caggtgacca
      181 gcgtcctcat cccggtgagt gggggcgggc tctcggcggg gatcgccacc gcggtgaagt
      241 cgctcgcccc gtcggcgcgg atcgtcgggg tcgagccgga gctggccgcc gacgccaggg
      301 agtcgctggc cgccggcgag atcgtccagt gggacgcgtc gctgaccacc cgcacagccg
      361 cggacggcct gcgcgtcgag cacctcggct ggctgccctt cgtccacctg cggcgctaca
      421 tcgacgagat cgtgaccgtc agcgaggagg ccatgaccga tgccattcgc cgcctggcct
      481 ccggcgcgcg gctggtggtc gagccgtccg gcgcggccgc catggctgcc cacctaaccg
      541 gcgccgcccc gcagccggac ggcgacgacc accgcgtgat cgtgatcagc ggcggcaaca
      601 tcgacccgaa gtcgctggcc gagatcctgg cctcgggctg accctcaggc cgacccgtcg
      661 agcccctgcc gcaggccggc caggaaggcc acggcgttgg caccgatggc atccaccgcg
      721 tagccgccct cctggacggc gatcacagag ccggccgagc cgccgctcgc ggtgctgccg
      781 atcatgcggc ccacctcggc gtagtcggcg gtgcgcagcg ccaggtcgga gatcgggtcg
      841 cgctcgaagg tgtcgaagcc gatggagacg accagcggcc cgtcgggatc gaagtccgcc
      901 gcgcggtcca gggccgcggc cagcgctgtg gcgtaggcgt ccagggtcgt gcccgccggc
      961 agcggaaagt tgagcgtgga cccctccccg gggccgccgc cgcgttccgc gctggagccg
     1021 gagaagtacg ggtaggcgcg cgccgggtcg ccatgcaggg aggcatacag cacgtccggc
     1081 cgctcccaga agatctgctg ggtgccgttg ccatggtggt agtcgacgtc gacgatggcg
     1141 acccggcgcg cgcccgcctc gcgccgcagc cactcggcca cgatggccgc gttgttgaag
     1201 tagcagtagc cgcccagcat gccccgcgcc gcgtggtgcc ccggcggccg gcacagcccg
     1261 taggccagcg ccgcgtcgcc gtgcgccacg gccgcggcgg cggagagggc cacgtcaacg
     1321 gcggcgtggg cagctgctgc cgtgccagcc acgattgggg tggcggtgtc gaagcagaag
     1381 gcgccgaggc gcaggtgagc ggcatcgggg agtggcagcc gcaacattgg tccggcgtag
     1441 gcgtccagcc ggaaggtgtc cgggatgagg gggcgcgact cgtccgcgcc ggccgccacg
     1501 gcatcggtcc aggcccgatc caccacctcc agcagctccg ggtcgtggac cgccaggatc
     1561 ggctcgcggc cgaactcgcg cggctcctcc aggcggaagc caccggcggc gagcagggca
     1621 cgctcgatct cgacagcgcg ctgcggcgac tcccagcagg tgacctcgcg cccggcgttc
     1681 acctccacca ggccgctgtg gccaaggtgg tcatcggtcc ggaagacggg aattcgggag
     1741 tcgcggtcgg ccatccgcgg gagtgtagcg gtggactcag ccggtacact cggcggaccc
     1801 atgtccacgc tcgacgacca ggcggcgctc gaggccctgc gcggctgtcc gctgttcgcg
     1861 ccgtgccccg agccggtgct ggccgaggtg gggcgccgcc tgcgtcaccg ccacttccgg
     1921 cgcaacgagg tcatcttcca ccagggagac ccgggcgacg cgctgcacgt catcacctcc
     1981 ggctcggtca agatcgtgct cccctcgccg gagggcgagg aggcgatcgt cgccaccctg
     2041 cggcccggcg acttcttcgg cgagctggcg ctgctcgacg gcaagccgcg ctcggccagc
     2101 gccgtggcgc tggaacagac cgagaccctg tccctgcccc gcgacgtctt ccgcgagctg
     2161 ctgaaccagc accccgagct gtgcgacgcc ctgttcgcca gcctggcgac cctgctgcgt
     2221 cggatcaccc gccatgtgga ggagctgcac ttcctggatc ttgccggccg cctcgcggca
     2281 cggctggccg aactggcgcg ccaggcccag ccacgcggcg ccggcctggg ccccgaggcg
     2341 gggccgatcg agctggactg gccctacacc cagagcgacc tggcgtccat gatcggtgga
     2401 acccgacaga gcgtgaaccg cctgctggcc gagctgatgg acgacggcct ggtgcggctg
     2461 gagcacgagc gactgatcgt gaacgacctg cgcgccctgg agcgccgcgc ggagcgctga
     2521 cgacgcccgg gccggtgctg tctagtggac tgccgctccc cggccccagc gctcgagctc
     2581 ggcgtcgagg tccaggggct tgacgacggc ctgctcggcg tactcgcccc atgcctcgcg
     2641 cggcacgcgc cacatcacct cgaactcgtt gccatccggg tcgcggccgt agagggactt
     2701 cgaaacgccg tggtcactcg ccccgcccat ggcgcccatc tcggacaggc ggccggccat
     2761 ctcggccagc ccctcgatcg actccacttc ccaggccagg tggtagagcc ctaccgaccc
     2821 gcgcggcggg cgcgccgcag cctgcccgac ctcgaacagg ccgaggtcgt ggtggttgtc
     2881 gccggcgggt gagcgcatga agaccgcccg catgccggcc ggtcgctcga tctcctcgaa
     2941 gccgaaggcg tcgcggtaga aggcggacgc gcggtcgacg tcgcgcacga agagcaccgc
     3001 gtggttaagc cgggtgactg cggcggccac ctcaggcctc ctgccgcagc agcttccagc
     3061 cgctgcgcca cggccagctg ttggccatgt tcaggctgat gttgacccag gccatgccct
     3121 ccaggacgcg ggccatcttc agctcgccga tgtccagcgg ctcgaagccc acgtcgccga
     3181 ccagcctgcc aaccaccttc ttggcctccg gatcatcacc cgccaggaag ccgtccaggg
     3241 acagcccacc atcgaaggtc gggtccgcca tgcggctggc gaaggcggtg ttgaaggcct
     3301 tgaccaccct ggcctcgggg agctgctcct ggacggtctc ggccaggctg cgtccctcct
     3361 ggacggccat cggcccgttg ctgacgtcca ccaccggctt gccgctcacg tgtccgcgca
     3421 gctcctgcgc gacctcgccc agcgcatcgg ccgggaccgc caaaacgacc gcctccgcct
     3481 tctcggccgc ttccgccggc gtcgcggccg gctcgcagtt caggtcctcc gccgcccgct
     3541 cggcgctctc ttcggtcgag gccgccacga ccacctcgtg cccggcggat tcgagcttct
     3601 ccgaaagggt gcttcccacg tttccgatac cgatgactgc cacacgcatg gtgaccaact
     3661 cctttgggta actaattgct tgtgcaacta tctcacgttc cggcagcgca cgcaagggaa
     3721 ggatggaagg ctgcaagcgc ggggccgcgg cgaccaaagt gaacggtcgc gattcacgcc
     3781 aggcgaccct cgcgggccga ggtcggcgag cctccctaga attggggagg cgaacgcagg
     3841 cagaggacgc tcagtgttct acgtcaagcg cggcgaggtg ccgcacaagc gccacaccca
     3901 gttccgcaag ggcgacggct cgctctacgc cgaggagctg ttcggggtgg agggctttgc
     3961 cggccgctcc tcgctcctct accaccacac cccgccgacc cagacccacc agatcgacga
     4021 cccgtcatcc gtccgcctgg agccggcgga ccccgagggt ggctcgccgc atcggcacca
     4081 cctggtcaac accgcccagc tggccgcgtc cggcgacggc ctgtcgggtc gcatcccgct
     4141 cttctacaac agcgacgtca ccttcggcgt ggtcctcccc gaggggccga tgaccgactt
     4201 ctaccgcaac ggcgtggccg acgagatgta cttcgtccac tccggcagcg gcacgctgga
     4261 gaccaacttc gggccgatcc cctatggccc tggcgactac ctggtgatcc ccatcggcac
     4321 cacctaccgc ctggtccccg atggcggcgt cgcccagcgc atgctgtggc tcgagtgccc
     4381 gtccgagatc gagccgccga agcgctaccg caacgagtac gggcagctcc tggagcactc
     4441 gccctactac caccgcgaca tccgcccgcc ggacgagatg ccgccggtga ccgatgccga
     4501 cggcgatcac gtgatcctgg tccgcagccg cgaccgcatc
//
LOCUS       DATACA010000197         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2516637, whole genome shotgun sequence.
ACCESSION   DATACA010000197 DATACA010000000
VERSION     DATACA010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2516637"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VHK28_04045"
     CDS             complement(<1..1319)
                     /locus_tag="VHK28_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX2141435.1"
                     /translation="MSRRPPYRSARPQPGGRGAPPRPGARAATRGRGPAALPIVILGA
                     FAALGIALFIGLLGVYASYTVGLANPADLEDFDLDEASTVLSADGTELATFATERRLP
                     IAFEDIPEVMVNAQVAAEDQSFWTNPCIDFRSIVRAALQNLTADDIVSGASTICQQLV
                     RIRLLDADLMGDPDRIFERKIKEAILALEVGEEFDGQEGKEKILEMYMNQVYYGNLAY
                     GVRAAAEAYFGKDITSDAPEEQLTIGEAAMLAGLVRSPSVLDPTHEAIEQTDADGNTI
                     LVVPETAGAERVQGFVLDNMIESGYITQAEAQEADAQEIVLAPPRDTDYRAPHFVYAV
                     RREAQRLLGNEELLDRGGLRIETTLNYDGYQVFAEKWAAVAYDMDRMTDEELLAKYGE
                     VALEWIKRLQGRNIQNDALVTLNYRTGAVLAYVGSANYYGEATPAHQP"
     gene            1381..>2124
                     /gene="tyrS"
                     /locus_tag="VHK28_04050"
     CDS             1381..>2124
                     /gene="tyrS"
                     /locus_tag="VHK28_04050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2141436.1"
                     /translation="MNGHILDELRWRGMVALSTDEQALRTAFAAGPVAFYCGFDPTAP
                     SLHFGNLVQLVTMRRLQDAGHRPIAVVGGATGLVGDPSGRSAERVLNEAEVVAGWVER
                     IRGQVERVLDFGGENPARVVNNLDWTAPLGALDLLREIGKHFSVARMLEKESVSARME
                     AGGISYTEFSYQILQANDYLELYRRHDCVLQTGGSDQWGNLTAGVDLIRRVEGVTVHA
                     LATPLLTKADGTKHGKTAEGSVWLDPEMMS"
BASE COUNT          316 a          704 c          756 g          348 t
ORIGIN      
        1 ggctggtggg cgggggtggc ctccccgtag tagttggcgc tgcccacgta ggccagcacg
       61 gcgccggtgc ggtagttgag ggtcaccagc gcatcgttct ggatgttccg tccctgcagc
      121 cgcttgatcc actcgagggc gacctcgccg tacttggcca gcagctcctc atcggtcatg
      181 cgatccatgt cgtacgccac ggcggcccac ttctcggcga agacctggta gccgtcgtag
      241 ttgagcgtgg tctcgatgcg caggccaccg cggtccagca gctcctcgtt gccgagcagc
      301 cgctgcgcct cgcggcgaac ggcgtagacg aagtgcggcg cgcggtagtc ggtgtcgcgc
      361 ggcggcgcca gcacgatctc ctgggcgtcg gcctcctggg cctcggcctg cgtgatgtag
      421 ccggactcga tcatgttgtc caggacgaag ccctgcactc gctcggcgcc cgccgtctcg
      481 ggcacgacca ggatggtgtt gccgtcggca tcggtctgct cgatcgcctc gtgggtcggg
      541 tcgagcacgc tgggggagcg caccagcccg gccagcatgg ccgcctcacc gatggtgagc
      601 tgctcctcgg gcgcgtcgga ggtgatgtcc ttgccgaagt aggcctcggc cgctgcccgg
      661 acgccgtacg ccaggttccc gtagtacacc tggttcatgt acatctccag gatcttttcc
      721 ttaccctcct ggccgtcgaa ctcctcgccg acctccaggg ccaggatcgc ctccttgatc
      781 ttgcgctcga agattcgatc cgggtcgccc atcaggtcgg catcaagcag ccggatgcgc
      841 accagctgct ggcagatggt cgacgcgccg gagacgatgt cgtcggcggt caggttctgg
      901 agggcagccc ggacgatgct gcggaagtcg atgcaggggt tcgtccagaa gctctggtcc
      961 tcggcagcca cctgcgcgtt gaccatcacc tcggggatgt cctcgaaggc gatcggcagg
     1021 cgccgctcgg tcgcgaaggt ggcaagctcg gtgccgtcgg ctgacaggac ggtcgacgcc
     1081 tcgtccaggt cgaagtcctc aaggtccgcc gggttggcca gccccaccgt gtaggacgcg
     1141 tacacaccca gcaggccgat gaacagcgcg atcccgagcg cggcgaacgc gccaaggatg
     1201 acgatgggga gcgccgccgg gccgcggccg cgcgtggcgg cacgggctcc gggtcgggga
     1261 ggggctccgc ggccgcctgg ctgcggccgg gcgctgcggt acggaggtcg tcgggacatc
     1321 ggtgcgtgac tctagcacgc atgccttcgg aggtccgatg gtccgtatac tcttgcgccc
     1381 atgaacggcc acatcctgga cgagctgcgc tggcgcggga tggtggcgct gtccaccgac
     1441 gagcaagccc tgcgcacggc attcgcggcc gggccggtag ccttctactg cggcttcgat
     1501 cccaccgcac cgtcgctgca cttcggcaac ctggtgcagc tggtcaccat gcggcgcctc
     1561 caggacgccg gccaccgtcc gatcgccgtc gtcggcggcg cgaccgggct ggtcggcgat
     1621 ccgagcggac ggtcggcgga acgggtgctg aacgaggccg aggtagtcgc cggctgggtg
     1681 gagcgcatcc gcggccaggt ggagcgcgtg ctcgacttcg gcggcgaaaa cccggcacgg
     1741 gtggtcaaca acctggactg gacggcaccc cttggcgctc tcgacctcct gcgcgagatc
     1801 ggaaagcact tcagcgtggc tcgcatgctg gagaaggagt cggtcagcgc ccgaatggag
     1861 gccggaggca tcagctacac cgagttcagc taccagatcc tgcaggccaa cgactacctg
     1921 gagctgtacc gccgccacga ctgcgtgctg cagaccggtg gctcggacca gtggggaaac
     1981 ctgacggccg gcgtggacct catccgtcgc gtggagggcg tgaccgtgca cgcgctggcc
     2041 acgccgctgt tgaccaaggc ggacggtacc aagcatggca agacggcgga gggctcggtc
     2101 tggctggacc cggagatgat gagc
//
LOCUS       DATACA010000198         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2596881, whole genome shotgun sequence.
ACCESSION   DATACA010000198 DATACA010000000
VERSION     DATACA010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596881"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VHK28_04055"
     CDS             <1..373
                     /locus_tag="VHK28_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006957342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2141437.1"
                     /translation="FESFDFAAVPMISKAHLQALATGDAWLDQGANVLLFGPPGSGKS
                     HCGAALGHALVGNGYRVLFARTTDLVQRLQAARRDLALEAAIARLDKFDLIVLDDLCY
                     ARKDQAETSVLFELIAHRPSN"
     gene            444..827
                     /locus_tag="VHK28_04060"
     CDS             444..827
                     /locus_tag="VHK28_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141438.1"
                     /translation="MAVVRRKRYQGEPHLVIERPDGGRQLLAARCVEPAATIPHATAA
                     PLVFTPGSLRLLANLVATLRSTPSPAPEARDAPRHRPIAAAPAAVEHLPARDPPAAGR
                     AVDRPAAAADPDRPGAGTRAVGAVP"
     gene            824..>2016
                     /locus_tag="VHK28_04065"
     CDS             824..>2016
                     /locus_tag="VHK28_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX2141439.1"
                     /translation="MNALSAKIQPRHLRRRAVVYVRQSTPRQVINNQESTRRQYRLAE
                     RARQMGWTAGQIEVVDDDLGLSGASSQARLGFQRLVAAIGLGEVGIVLVTEISRLSRR
                     NSDWHRVIELCAVFRTLIADEDGVYDAQDPNDLLLLGVKGTLFAAELHILHARMRGNL
                     LNKARRGELALRLPVGYRRLGDGTVVPEPDEQVRATLALLFERFALVRNARAVQRYFL
                     EHGLAMPRLIQQGAEAGRIAWVRPTYQMIQQVLTSPVYAGVFVYGRRQRQVAPGDPPI
                     VTDRRRPVEEWDIVVPGIYPAYLSYDQYLLNRRQLHDNLYNFAKKGRGAPREGRALLQ
                     GLVVCGRCGRRMAVSHGSRYHRYECRRAQLDYAAPQCQAFPVAYLDRAVGALFLAAVA
                     PAALET"
BASE COUNT          289 a          756 c          683 g          288 t
ORIGIN      
        1 cttcgagagc ttcgacttcg ccgcggtgcc gatgatcagc aaggcgcacc tgcaggccct
       61 cgccaccggc gacgcctggc tcgaccaagg ggccaacgtg ctgctgttcg gcccacccgg
      121 cagcggcaag agccactgcg gcgcggcgct cggccacgcc ctggtcggga acggctaccg
      181 ggtgctgttc gcgcgcacca ccgacctcgt tcagcgcctc caggccgccc gacgcgacct
      241 cgccctcgag gcggcgatcg cccggctcga caagttcgac ctcatcgtcc tcgacgacct
      301 ctgttacgcc cgcaaggacc aagccgagac ctcggtgctg ttcgagctga ttgcacaccg
      361 gccatcaaac tgagcggttg cgttccagcc acgtcgaatg cgtgcgggtc atcgcggcgc
      421 accacccgct agcaggccag ctggtggccg tggtgcggcg caagcgctac cagggtgaac
      481 cgcacctggt gatcgagcgg cccgacggtg ggcggcagct gctggcggcc cgctgcgtgg
      541 agccggctgc caccatccca cacgccacgg ccgcgccgct ggtcttcacc ccgggcagcc
      601 tgcggctgct ggccaacctg gtcgccacct tacggagcac accatccccc gctccggagg
      661 cccgcgatgc tccccggcac cggcccatcg ccgccgctcc tgctgctgtg gagcaccttc
      721 cagcccgaga cccaccggca gctggtcgag ctgtggaccg acctgctgca gcggcagatc
      781 ccgatcgccc aggggcgggc acccgagccg tgggagccgt gccatgaatg ccctgtccgc
      841 caagatccag ccgcgccatc tgcgtcggcg cgccgtcgtc tacgtccggc agtcgacgcc
      901 ccggcaggtg atcaacaacc aggagagcac ccgccggcag taccggctgg ccgagcgggc
      961 gcggcagatg ggctggacgg ccggtcagat cgaggtcgtc gatgatgatc tcggcttgtc
     1021 cggcgccagc agccaggccc ggctcggctt ccagcgcctg gtggccgcaa tcggcctggg
     1081 cgaggtcggc atcgtgctgg tcaccgagat ctcgcgcctg tcccggcgca acagcgactg
     1141 gcatcgcgtg atcgagctgt gcgccgtgtt ccgcaccctc atcgccgacg aagatggcgt
     1201 ctacgacgcg caggacccga acgacctcct ccttctgggc gtcaagggca cgctgttcgc
     1261 cgccgagctg catatcctgc acgccaggat gcgcggcaac ctgctcaaca aggcccggcg
     1321 gggcgagctg gccctgcgcc tgccggtcgg ctaccgccgg ctcggcgacg gcacggtcgt
     1381 gcccgagccg gacgagcagg tgcgcgcaac gctggcgctg ctgttcgaac ggttcgccct
     1441 ggtgcggaat gcccgcgccg tgcagcgcta cttcctcgag cacgggctgg cgatgccgcg
     1501 gctgatccag cagggagccg aggccggacg catcgcgtgg gtccggccca cctaccagat
     1561 gatccagcag gtgctcacca gcccggtcta cgccggcgtg ttcgtctatg gccggcgcca
     1621 gcggcaggtc gccccgggcg acccgccgat cgtcaccgac cggcgccggc cggtcgagga
     1681 atgggacatc gtcgtgccgg gcatctaccc ggcctatctc agctacgacc agtatctcct
     1741 caaccggcgc cagctgcacg ataacctcta caacttcgcc aagaagggcc ggggcgcgcc
     1801 gcgcgagggt cgcgccctgc tgcaggggct tgtggtctgc ggccgctgcg ggcggcggat
     1861 ggccgtcagc cacggcagcc ggtatcaccg ctacgaatgc cgccgggcgc agctcgacta
     1921 cgccgccccg cagtgccagg cattcccggt ggcctacctg gaccgggccg tcggcgcgct
     1981 gttcctggcg gcggtggcgc cggccgcgct ggagac
//
LOCUS       DATACA010000199        10973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_187770, whole genome shotgun sequence.
ACCESSION   DATACA010000199 DATACA010000000
VERSION     DATACA010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187770"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VHK28_04070"
     CDS             complement(<1..1284)
                     /locus_tag="VHK28_04070"
                     /inference="COORDINATES: protein motif:HMM:NF023650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HEX2141440.1"
                     /translation="MTTLTALARPRVLLATGPLRRGFLLGLFGTLLVASFLAVGLTAG
                     VGLSHADRIMPGVRVAGVELGGLSRSAAAARLEASLPSLTAGTLNVAVDGVDVEVPVA
                     DIGRAYQVEAMLERAFAVARDGNPLADAIDRIRLLARPPELSAASAGTADAVDRVTQG
                     LVARFAVAPVDGSVTYDPENGFVAVPASDGAALDATEVRAALAAFAATPSTAPTSLSF
                     QTSPVAPQISTRDAGAAAIAARWMTATPLALEAAGKPLSLEPAALAGALTFGTRDGVY
                     GPIVVEAALTALVEPLAPQVASAAKDATFTWNANGISGVVPGATGRELDVAGSVRAVA
                     DALRTRGGGSLTPSAALSVSVTQPALTTENAQAAMAKMTRMSTWTTFYTPGESNYWGA
                     NISIPARDLDTLVIAPGEWFSFWNDIGPVTVARGYG"
     gene            1569..1922
                     /gene="rpsT"
                     /locus_tag="VHK28_04075"
                     /pseudo
     CDS             1569..1922
                     /gene="rpsT"
                     /locus_tag="VHK28_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
     gene            complement(2162..3472)
                     /gene="eno"
                     /locus_tag="VHK28_04080"
     CDS             complement(2162..3472)
                     /gene="eno"
                     /locus_tag="VHK28_04080"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX2141441.1"
                     /translation="MTTLIEDVIAREVLDSRGNPTVEVEVFLDGGAVGSAIVPSGAST
                     GAHEAVELRDGDRSRYGGKGVLTAVKNVNEVVRPEVTGSDAVDQVSLDRLLIDLDGTA
                     NKASLGANALLGVSLAAAHAAAESMSLPLYRYLGGVNARVLPVPLVNILNGGKHAIDS
                     TDFQEFMIAPLGAPTFREGLRWAAETFHALGALLHERGFATTVGDEGGYAPSLESNEA
                     AISLVVEAIERAGYAPGEQVAVALDPATTELYDDGAYALAREGRTLTTDELVDFWVDW
                     SERYPIVSLEDGLAEDDWDGWRALTERAGERLQLVGDDLLVTNTERLARAIEMRAANS
                     ILVKLNQIGTLTETLEAVEMAHRAGWTAVISHRSGETEDTTIADLVVALNTGQIKTGS
                     ISRSERIAKYNRLLRIEEELGDAAQYAGRRAFAAMRGDGSGQGA"
     gene            complement(3469..4683)
                     /locus_tag="VHK28_04085"
     CDS             complement(3469..4683)
                     /locus_tag="VHK28_04085"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX2141442.1"
                     /translation="MNKKTIRDIDVRGKRVFLRADLNVPLDDGRITDDTRIRASLPTL
                     VYLLEHGATVILASHLGRPKGKVNDALRLKPVADRLGQLLGRPVRMTGDALGPGVKTA
                     VDKLRPGDMLLLENLRFHAEEEANDPEFAKALADMADVYVNDAFGSAHRAHASTEGIT
                     HFLPAVAGLLMEREVSSLSRLMERPAKPFHTVIGGAKVSGKLEVLEFLLSRCQAVLVG
                     GGMANTFLAAAGHALGKSLVEADQLDAASRIVTEARRKRVRFMLPTDVVIAAQVHPRS
                     QRQVVSVSEVPKDWSVVDIGPGTISAYTEHLMKARTIFWNGPMGIFEIAPFAEGTNAM
                     ARFLAERTDAGVTTVVGGGDSVAAVEQLGLAERMSHVSTGGGASLEFVEGKTLPGVAA
                     LLDADAPVEMAR"
     gene            complement(4697..5701)
                     /gene="gap"
                     /locus_tag="VHK28_04090"
     CDS             complement(4697..5701)
                     /gene="gap"
                     /locus_tag="VHK28_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX2141443.1"
                     /translation="MTRIAINGFGRIGRQTLRAVIERQPDLEVVAVNDLAPTQTNAHL
                     FKYDSTYGRYEGEVSAGEGRITVDGREIRAHSEKDPAALPWKDLGVDIVVESTGIFTD
                     ATRARAHIDAGARKVIISAPAKNEDVTLVLGVNEGTYDPEQHHVVSNASCTTNGLALP
                     AKVVFDSFGIQRGLMTTIHSYTNDQNVLDVFHKDLRRARSAGQNIIPTTTGAARALSL
                     VIPELKGKFDGFALRVPTPTVSVIDFVAITERPVTAESANRALREAADGPMQGLLGYT
                     EEQLVSMDFKGDPRSSIVDAASTMVTGDNLLKVIAWYDNEWGYSCRVADLAKYMADRL
                     "
     gene            complement(5712..6734)
                     /locus_tag="VHK28_04095"
     CDS             complement(5712..6734)
                     /locus_tag="VHK28_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX2141444.1"
                     /translation="MDESAERPILAIDLGGTRIRTAYITSQLAVACRRAADTRDEDGL
                     DVVLERISRLASESLAEARANRLPEPAGIGISSPGPLDPWRGVVLAPPNLAGWRNVPL
                     ADRVEQALGLPTFLERDTNVAVMAEWRHGAARGAQDVIYITISTGVGGGIIIGGRPLI
                     GADGTAGEVGHLTVELDGPLCGDGAPGHVEAIASGTAIARDGRQLLLDGRSPGLAALT
                     DDPDAVDARLVGQAADAGDPACQALLARAWTAVGALCSGLVNLLNPEVIVLGGAIALN
                     RPEMLGVIGDELARRAFPTPARRVRVVLSEHGDDVSLLGSLPIVNERLDDPAYRRDRH
                     PTPLRR"
     gene            complement(6908..7051)
                     /locus_tag="VHK28_04100"
     CDS             complement(6908..7051)
                     /locus_tag="VHK28_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX2141445.1"
                     /translation="MATEAYCVKCKAKRQMTNEQKITMKNGKPATQGTCPVCGTKMFK
                     IGG"
     gene            complement(7066..7941)
                     /locus_tag="VHK28_04105"
     CDS             complement(7066..7941)
                     /locus_tag="VHK28_04105"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEX2141446.1"
                     /translation="MTDRTSADSPTTGGRQYHIALERGELAEYILLVGDPGRVAKVAE
                     RWDSVELERSNREITSATGTHRGMRISALSTGMGTDNVEIVFAEVTEITDRPTFVRIG
                     SSGALQPEIGLGELIISTGAVRLENTSDYYVHPGYPAIAHREVVWALEAACRQLGVTY
                     HVGLTATASGFYAPQGRAMRTLPVRYPELAEELRRQRVANLEMESSTLFVLAGLAGLR
                     AGTICAAYAQRIDGTFVEGAAKEAAEARCIDAGLSGIHLLWTMDQQQEGDPRHAWSST
                     IGQGGNQRHTGTSRK"
     gene            complement(8017..9711)
                     /locus_tag="VHK28_04110"
     CDS             complement(8017..9711)
                     /locus_tag="VHK28_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141447.1"
                     /translation="MANGSSVVVEAVSLTDGSFSEGGGYVVDSSGGIAVLVSDGTFPR
                     AASLRVSGTVDDRFHQRTIRAEAAGVEVLGTASDPEAAAAVATGSVGEGIEGQLAEID
                     GTILGAPTSLSAGLAFDVDDGSGSLRVVVGTHTGIDTADWEAGSRLIVRGVVGQRDSS
                     GSGTAGYRLQPRDSADVIQLSATPAPTGSPPPGAGSPSPSPSEPADPGVISIAGARAA
                     PSGSRLTVTGVVTLPTGLSEPGSGVIQDSSGAILLRVSDEAGDLLRGQLVRVSGVRST
                     WSGMLSLRVSDAPTVLGSQADPVAARRSTGAIGEAEEGLLIVARGLVTGAPRRTSAEN
                     TYLDLDDGSGALRVFLSPRSGIGPGAILAGAWLEVTGVLTQETTGKQPERGYRLWPRN
                     AADVRVVASPVGDATSDAGTPGGAGGRSPSGGPRSGPAAPDPGHLVDVAFPPKPRLAL
                     ALPTASPPAALPHVDSAPAVAVGPNDQSQVLLLLAAAFALLAAASMVITPGLVPRLRE
                     LLADGGPSPTEAGGSAGQPPGSALGAERGGLAVLTVLDGFGPSGGGGRDRPPPSVR"
     gene            complement(9669..10511)
                     /locus_tag="VHK28_04115"
     CDS             complement(9669..10511)
                     /locus_tag="VHK28_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX2141448.1"
                     /translation="MAAALAALALLASVLSTAAASVERWNAVGVGFAADPLVAAEDAH
                     LVVSEVQTGGAGASDEFIEIYNPSPFALPLEGLEVVYVSASGLSVTRKAAWEAGNPPL
                     NPGAHLLIANEAGIYASLADVPYANGLAATGGSVAIRIVGASTAVDAVGWGSAASTWL
                     EGTPIQAPPAGASIERLPGGASGSGQDTDHNVVDFVVRDLPDPQNLGSSPIPVGSPEP
                     TPSGTANPSVAPSATPSASRCRRPRRPHWLCQAPRPRPPQPSRSRRPARWRTVRPWWS
                     RRSH"
     gene            10699..>10973
                     /locus_tag="VHK28_04120"
     CDS             10699..>10973
                     /locus_tag="VHK28_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141449.1"
                     /translation="MSNRSRREAEARAEARRRARYQARGVPVEEPDEEASEADERRES
                     RAPAAGGGFLTRLFPPAPPLPNRPDPLRNFSYSGPLRGVVAGLYLLMR"
BASE COUNT         1556 a         3929 c         3873 g         1615 t
ORIGIN      
        1 cccgtagccg cgagcgacgg tgaccgggcc gatgtcgttc cagaagctga accactcgcc
       61 gggagcgatg accagggtgt ccaaatcccg ggccgggatg ctgatgttgg cgccccagta
      121 gttgctctcg ccgggcgtgt agaaggtggt ccaggtcgac atgcgcgtca tcttggccat
      181 cgcagcctgc gcgttctccg tggtcagggc tggctgcgtc acgctcaccg agagcgcggc
      241 ggatggggtc agtgagccgc cgccgcgcgt ccgcaaggcg tcggccacgg cacgcacgct
      301 gcccgcaacg tccagctcgc ggcccgttgc gccgggcacc acgccggaga tgccgttggc
      361 attccaggtg aaggtggcgt ccttggccgc cgatgcgacc tgcggcgcca gcggctcgac
      421 gagcgcggtg agggccgcct cgacgacgat gggcccgtac acgccgtcgc gggtgccgaa
      481 ggtgagcgcc ccggccaggg cagccggctc caggctcaag ggcttgccag ccgcctccag
      541 ggccagcggc gtggccgtca tccagcgcgc ggcgatggct gccgcgccgg cgtcccgggt
      601 gctgatctgg ggagcgaccg ggctggtctg gaacgagagc gacgttggcg cggtcgatgg
      661 cgtggcggcg aaggctgcca gcgcggcgcg aacctcggtg gcatcgagcg cggctccgtc
      721 gctggcgggc acggccacga acccgttctc gggatcgtag gtgacactgc cgtccaccgg
      781 cgccaccgcg aagcgggcta ccaggccctg cgtgacccgg tcgaccgcgt ccgccgtccc
      841 cgcggacgcg gcgctcagct cgggcggccg ggccaggagg cggattcgat caatggcatc
      901 ggccagcgga ttcccatccc gagccaccgc gaatgcacgc tccagcatgg cctcgacctg
      961 gtacgcgcga ccgatgtcgg cgaccggcac ctccacgtcc acgccgtcga ccgccacgtt
     1021 cagggtgccg gcagtgagtg acgggagcga tgcctccagc cgggcggcgg ccgcggaccg
     1081 gctcagcccg cccagctcga caccggcgac gcgaactcca ggcatgatcc gatcggcgtg
     1141 gctcagtccc acgccggcgg tgaggccgac cgccaggaag ctcgcgacca gcagcgtgcc
     1201 gaacagcccc agcaggaaac cgcggcgcaa cgggccggtt gcgagcagga cgcggggtcg
     1261 ggcgagggca gtgagtgtgg tcatggtcct ttcgacgacg agggtgccag ccggccactg
     1321 cggcgaggct gcggtgaacc gcagccggga tccgtcgcga gtggcactct cggttccttc
     1381 cctcccagac gccgcacccg ggcgtatcgt tgcaccggcg accaagggca tgcggtcgcc
     1441 gctcgcgcga ttctgccgcc tggcaacctt cctgtcgagt ggcactttgg tgtggttccc
     1501 tgcattctgc gggccgcgct cggctcggga cccatggttg gccggaacga gctcgcgtgc
     1561 tacactccat gaccgatggc gccgctccgc ggcgctcgtt tcatgcacac acccaaccac
     1621 tggctcgagg atcttccgtg gcaagaaagg cacgtacctg gaagggcgcg cgcaccccgc
     1681 aggcgctcaa gcgcgaccgc caggcactgc ggcgccgtag cgtcaaccgt cgcgctcgga
     1741 gcgaggccaa gacgctcgtg cagcgcgcca acagcatcgc gctcggccga gccgagggtg
     1801 atggccccga ggcgatcacc gccgccgtca gcgcgctcga tcgcgccgcc gagaagggca
     1861 tcatccaccc gaacaacgcg gcccgccgca agagccggct gatggccaag gccaacgccg
     1921 cgcacctgct ggatggcgcc gcgacggcgg agcccaccgc gcgcaagcgg acggtggcca
     1981 ccaagaccgc gcagcgcaag aaggtggccg ccgccaaggc gtccaaggcc gccgcggccc
     2041 ggacggcaga caagccgcgc accgcggccg gcaaggcgaa ggtggcggtc acgcgcgcgt
     2101 cgcgagagtc tgcggcggct cgccgccgca agcaggcacc cggcgaggac agctccgagg
     2161 gctaagcccc ctgcccggac ccgtcgccgc gcatcgcggc gaacgcgcgc cggccggcgt
     2221 actgcgctgc gtcgcccagc tcctcctcga tacggagcag ccggttgtac tttgcgatgc
     2281 gctccgaacg gctgatgctg ccggtcttga tctggccagt gttgagggca accaccaggt
     2341 cggcgatggt ggtgtcctcc gtctccccgg agcggtggct gatcactgcc gtccaccccg
     2401 cgcgatgcgc catctcgacc gcctccagcg tctcggtcag cgtgccaatc tggttgagct
     2461 tgaccaggat gctgttcgcc gcgcgcatct cgatggcccg cgccaggcgc tcggtgttcg
     2521 tcaccagcag gtcatccccc accagctgca gccgctcccc ggcgcgttca gtcagcgccc
     2581 tccacccgtc ccagtcgtcc tcggcgaggc cgtcctccag gctgacgatc gggtagcgct
     2641 ccgaccagtc cacccagaag tcgaccagct cgtcggtggt cagcgtgcgc ccctcgcggg
     2701 ccagtgcgta ggcgccgtcg tcgtagagct cggtcgtcgc cgggtcgagg gccaccgcca
     2761 cctgctcgcc cggggcgtac ccggcgcgct cgatggcctc caccaccagg ctgatggccg
     2821 cctcgttcga ctccaggctc ggcgcgtagc cgccctcgtc gcccacggtg gtggcgaagc
     2881 ctcgctcgtg gagcagcgcc cccagtgcat ggaacgtctc ggccgcccag cgcagcccct
     2941 cgcggaaggt gggcgcgccg agcggcgcga tcatgaactc ctggaaatcg gtgctgtcga
     3001 tggcgtgctt gccgccgttg aggatgttga ccagcggcac cggcaaaacc ctggcgttca
     3061 cgccgccgag gtagcggtac agggggaggc tcatcgactc ggccgccgcg tgtgccgcgg
     3121 ccagcgaaac gcccagcagc gcgttggcgc ccaggctggc cttgttcgcc gtgccatcca
     3181 ggtcgatcag cagccgatcc aggctgacct ggtccaccgc gtccgagccg gtcacctccg
     3241 gacggacgac ctcgttgacg ttcttgaccg cggtcagcac tcccttgccg ccgtagcgcg
     3301 agcggtctcc gtcgcgcagc tcgaccgcct cgtgagcacc ggtcgaggcg ccggacggca
     3361 cgatggcact gcccaccgct ccgccgtcca ggaagacctc gacctccacc gtcggattgc
     3421 cgcgcgagtc gagcacctcg cgggcgatga cgtcttcgat aagcgttgtc accgggccat
     3481 ctccaccggg gcgtcggcgt cgagcagggc ggccacgccc ggaagggtct tgccctccac
     3541 gaactccagc gaggcgcccc cgccggtact gacgtggctc atgcgctcag ccaacccgag
     3601 ctgttcgacg gcggcgaccg agtccccgcc cccgacaacg gtggtgaccc cggcatcggt
     3661 tcgctcggcc aggaatcggg ccatggcgtt ggtgccctcc gcgaatgggg cgatctcgaa
     3721 gatgcccatc ggtccattcc agaagatggt gcgtgccttc atgaggtgct cggtgtaggc
     3781 gctgatggtt cctggaccga tgtcgacgac cgaccagtcc ttgggcacct cgctgaccga
     3841 taccacctgg cgctgggagc gcgggtggac ctgcgcggcg atcacgacgt cggtgggcag
     3901 catgaagcgc acgcgcttgc gccgcgcctc ggtcacgatc cgggaggcgg cgtccagctg
     3961 atcggcctcc accaggctct tgcccagggc gtggccggcc gcggccagga aagtgttggc
     4021 catgccgccg ccgaccagca ccgcctggca gcgcgacagc aggaactcga gcacctccag
     4081 cttgccggac accttggcgc cgccgatgac cgtgtggaac ggcttagccg gccgctccat
     4141 gaggcgtgac agggagctga cctcgcgctc catgagcagg ccggccaccg cgggcaggaa
     4201 gtgggtgatc ccctcggtcg aggcgtgcgc gcggtgggcg gagccgaacg catcgttcac
     4261 gtagacgtcc gccatgtcgg ccagggcctt ggcgaactcc gggtcgttcg cctcctcctc
     4321 ggcgtgaaag cgcaggttct cgaggagcag catgtcgccc gggcgcagct tatcgaccgc
     4381 cgtcttgacg cctggcccga gggcgtcgcc ggtcatgcgc accgggcggc cgagcagctg
     4441 gccgagacgg tcggccaccg gcttgaggcg cagcgcatcg ttcaccttcc ccttcgggcg
     4501 gcccaaatgg ctggccaaga tcacggtggc gccgtgctcc agcaggtaga ccagggtcgg
     4561 caggctggcg cggatacggg tgtcgtcggt aatgcggccg tcgtccagcg ggacgttgag
     4621 gtccgcgcgc aggaagacgc gcttgccgcg cacgtcgatg tccctgatcg tcttcttgtt
     4681 catggtgtct ctccggctac agccggtcgg ccatgtactt cgccaggtcc gccacgcggc
     4741 agctgtagcc ccattcgttg tcgtaccagg cgatcacctt cagaaggttg tcgccggtga
     4801 ccatggtcga ggcggcatcc acgatgctgg accgcggatc tcccttgaag tccatgctca
     4861 ccagttgctc ctcggtgtag cccagcagcc cctgcatcgg tccatccgcc gcctcgcgca
     4921 gcgcccggtt ggcgctttcg gcggtgaccg gacgctcggt gatggccacg aagtcgatga
     4981 cgctcactgt cggggtcggc acccgcaggg cgaagccgtc gaacttgccc ttcagctcgg
     5041 ggatgaccag ggacagggcc cgggcggcgc cagtggtggt gggaatgatg ttctgcccag
     5101 cgctccgcgc gcgccgcagg tccttgtgga acacgtcgag cacgttctgg tcgttggtgt
     5161 agctgtggat ggtggtcatc aggccgcgct ggatgccgaa gctgtcgaag acgaccttgg
     5221 ccggcagcgc gagcccgttg gtcgtgcacg atgcgttgct caccacgtgg tgctgctccg
     5281 gatcgtaggt gccctcgttc acgcccagca ccagcgtgac atcctcgttc ttggcgggag
     5341 cgctgatgat caccttccgc gcgccggcgt cgatgtgggc gcgagccctg gtggcatcgg
     5401 tgaagatgcc ggtcgactcc accacgatgt cgacgcccag gtccttccag ggcagggcgg
     5461 ccggatcctt ctcgctgtgg gcgcggatct cgcggccatc gaccgtgatg cgtccctccc
     5521 cggcggacac ctccccctcg tagcggccgt aggtcgagtc gtacttgaag aggtgcgcgt
     5581 tggtctgggt gggcgccagg tcgttgaccg ccaccacctc gaggtccggt tggcgctcga
     5641 tgacggcgcg aagggtctgg cggccgatgc ggccgaatcc gttgatcgcg atgcgggtca
     5701 cgctgagctc ctcatctgcg caggggcgtg gggtgacggt cacgccggta ggcgggatcg
     5761 tcgagacgtt cattgacgat gggtagcgag ccgagcagcg acacgtcgtc accgtgctcg
     5821 gagaggacga cccgcacgcg ccgtgccggg gtcgggaatg ctcgacgcgc cagctcgtca
     5881 ccgatgacgc ccagcatctc gggccggttc agcgcgatgg cgccgccgag cacgatgacc
     5941 tccgggttga ggaggttcac caggccgctg cacaacgccc cgacggcggt ccaggctcgc
     6001 gccaggaggg cctggcaggc gggatcaccg gcgtcggcgg cctggcccac cagccgcgca
     6061 tcgacggcgt ccgggtcatc ggtcagtgcg gcgaggccgg gcgagcggcc atccagcagc
     6121 aactgccgcc cgtcgcgggc gatggctgtg cctgacgcga tcgcctcaac atggccggga
     6181 gccccgtctc cgcacaacgg gccgtccagc tccaccgtga gatgaccgac ctcgccggcg
     6241 gtcccgtcgg cgccgatcag cggccggcca ccgatgatga tgccgccgcc gacgccggtc
     6301 gagatggtga tgtagatgac gtcctgcgcc ccgcgggcgg ccccgtggcg ccactcggcc
     6361 atgaccgcca cgttggtgtc ccgctccagg aatgtcggca ggccgagcgc ctgctccacc
     6421 cgatcggcca gcgggacgtt gcgccagccg gccagattgg gtggggccag caccacgccg
     6481 cgccacggat ccagcggccc gggagaggaa ataccgatcc ccgcaggctc ggggagcctg
     6541 ttcgcacgcg cctccgcgag gctctccgac gcgaggcggc tgatccgttc cagcaccacg
     6601 tccagcccat cctcgtctcg ggtgtcagcg gcacggcggc acgcgacagc cagctgcgag
     6661 gtgatgtacg cggtgcggat ccgggtcccg cccaggtcga tcgccaggat cggccgttcg
     6721 gctgactcat ccacgaggag ccaattgtac cgccggcttt acggcgctca gagccgagtc
     6781 aactccgcgg cgcggcaggc gggcgtgaaa aggccgcccg gcggcggcca tcggccggtg
     6841 tgacccggtg ccgatgacgc gcggccggac ggccgacgcg tgagtgcgtt gactcaggtg
     6901 gcgatcgtca gccgccgatc ttgaacatct tggtgccgca caccgggcag gtgccctggg
     6961 tcgccggctt gccgttcttc atggtgatct tctgctcgtt cgtcatctga cgcttggcct
     7021 tgcacttcac gcagtaggct tcggtcgcca tcggttgttg cctccttact tccggctggt
     7081 gccggtgtgc cgttggttcc caccctgccc gatcgttgac gaccaggcgt ggcggggatc
     7141 gccttcctgc tgctggtcca tggtccacag caggtggatg ccggacaacc cggcatcgat
     7201 gcagcgtgcc tctgcggcct ccttcgccgc gccttcgacg aacgtgccgt cgattcgctg
     7261 cgcgtacgcc gcgcagatgg ttcctgcgcg aagtcccgca agcccggcaa gcacgaacag
     7321 cgtcgacgac tccatctcca ggttcgccac gcgttggcgc cgtagctcct ccgcgagttc
     7381 aggatagcga accgggagcg tgcgcatcgc acgtccctgc ggagcgtaga acccgctggc
     7441 ggtcgcggtc agccccacgt ggtacgtgac gcccagctga cggcacgccg cctccagtgc
     7501 ccagaccacc tcccgatggg cgatggccgg gtagccgggg tgcacgtagt agtcgctcgt
     7561 gttctccaac cggaccgccc cggtagagat gatcagctct cccagtccga tctccggttg
     7621 gagggccccc gaggaaccga tgcgcacgaa ggtcggccga tcggtgatct cggtgacctc
     7681 cgcgaacacg atctccacgt tgtctgtgcc catcccggtc gacagcgccg agatgcgcat
     7741 gccgcggtgc gtgccggtgg ccgacgtgat ctcccggttg ctccgctcca gctccaccga
     7801 atcccagcgc tcggccacct tggcgacgcg ccccgggtca ccgaccagca ggatgtactc
     7861 cgccagctca cctcgctcca gggcgatgtg gtactggcgg ccgccggtcg tcggcgagtc
     7921 tgcgctggtc cgatctgtca cgcggctctg gcgtctcctc gtggcgagcg tggttcacgc
     7981 tcgatcggcg ggagtatacg ccgagctcac gcacgatcag cgcacgctcg ggggcggccg
     8041 gtcccggccg ccgccgcccg acggcccgaa tccgtcgagc acggtgagga ccgccagccc
     8101 tccccgctcc gctccgaggg cactcccggg gggctgcccg gcggacccgc cggcctcagt
     8161 tgggctcggt ccgccatcgg ccaggagctc gcgcaggcgt ggcaccaggc ctggcgtgat
     8221 caccatgctc gcggccgcca ggagggcgaa cgctgcggcc agcagcagca acacctggct
     8281 ctgatcgttg gggccgactg ccacggcagg tgcgctatcg acgtgcggca gggccgcggg
     8341 cggcgaggcg gtgggcagcg ccagggccag gcgcggcttg ggcggaaagg cgacgtcgac
     8401 caagtgacca gggtccggcg cggccgggcc gctgcgtgga ccgccggacg gactgcgtcc
     8461 gccggctccc ccgggcgtgc cggcgtccga cgtcgcgtcc cccaccgggg aggccacgac
     8521 gcgcacgtcg gccgcgttcc gcggccagag ccggtacccg cgttcgggct gcttgccggt
     8581 ggtctcctgg gtcagcaccc cagtcacctc cagccaggca ccggccagga tcgcgccggg
     8641 tccgatcccg gagcgcgggc tgaggaagac gcgcagtgcg ccgctcccgt cgtccaggtc
     8701 gagataggtg ttctctgccg acgtgcgccg cggcgcgccg gtcaccagcc cgcgggcgac
     8761 gatcagcagc ccttcctccg cctcgccgat cgctccggtg gaacggcggg ctgccacagg
     8821 gtccgcctgg gagccgagca cggtgggcgc gtccgaaacc cgcaggctga gcatgcccga
     8881 ccaggtcgaa cgcacgccac tgacccgcac cagctggccg cgcagcaggt ctccagcctc
     8941 gtcgctcacc cgcagcagga tcgcgccaga ggagtcctgg atgacaccgc tccccggctc
     9001 ggacaggccg gttgggagcg tcaccacgcc ggtcaccgtc agccgtgagc ccgatggcgc
     9061 ggcgcgagcg ccggcgatgc tgatcacacc cgggtcagcg ggctcgctcg gcgacggcga
     9121 cgggctgcct gcgccgggcg gcggggaccc cgttggcgca ggcgtggcgg acagctggat
     9181 gacgtccgcg ctgtcgcgtg gctggaggcg gtagccggcg gtgccggagc cggaggagtc
     9241 gcgctggccg accacgccgc gcacgatgag gcggctgcca gcctcccagt ccgccgtgtc
     9301 gatgccggtg tgggtcccaa ccaccacccg cagcgagccg ctgccgtcgt ccacgtcgaa
     9361 ggccaggccg gcgctgagcg aggtcggggc acccaggatc gtgccgtcga tctcggccag
     9421 ctgcccttcg atgccctcgc cgaccgatcc ggtggccacc gcggcggcgg cctcggggtc
     9481 gctcgcggta cccagcacct ccacaccggc tgcctcggcg cgaatggtgc gctggtggaa
     9541 ccggtcgtcc acggtgccac tcacccggag cgatgctgcc cgcgggaagg ttccgtccga
     9601 gaccaggacc gcgatgccgc cactcgaatc gaccacgtac ccgccgcctt ccgaaaaact
     9661 gccgtcggtc aatgagaccg cctcgaccac cacggacgaa ccgttcgcca tcgcgcgggc
     9721 ctccgcgacc gagagggctg cggtgggcgt gggcgtggcg cttggcaaag ccaatgggga
     9781 cggcgtggcc gtcggcagcg ggaggcgctc ggcgtcgcgc tcggcgcgac cgatgggttc
     9841 gccgtgccgc tgggcgtggg ctccgggctg cccaccggga tcggcgagga gccgaggttc
     9901 tgtgggtcag ggagatcacg caccacgaag tccaccacgt tgtggtcggt gtcctggccg
     9961 gatccggaag cgccgccggg cagccgctcg atcgaggctc cggctggtgg ggcctggatg
    10021 ggcgttccct ccagccaggt gcttgccgcg gacccccagc ccaccgcgtc caccgctgtg
    10081 gaggcaccca cgattcgaat ggccacgctg ccgccggtcg ccgccagccc gttggcatat
    10141 gggacgtcgg ccagcgaggc gtagatgccg gcctcgttgg cgatcagcag gtgcgcgccc
    10201 gggttgaggg gcggatttcc ggcctcccag gccgccttgc gcgtcacgct gaggccgctg
    10261 gcgctcacat agaccacctc cagtccctcc aacggcaggg cgaacggaga agggttgtag
    10321 atctcgatga actcgtcgga cgcgcccgcc ccaccggtct gcacctcgct gacgacgagg
    10381 tgcgcgtcct ccgccgcgac cagggggtcc gcggcgaatc cgacaccaac cgcgttccac
    10441 ctctcgaccg atgctgcggc ggtggacagc acggacgcga gcagggcgag cgcggcgagc
    10501 gcggccgcca tgcgccgccg cggtctgcgg ggtggatggc tggcgggcac gggcaacctc
    10561 cttcggaagg aggcgcgcgg gcgggacgga tgaacgaggg caccagactg cccgatatcc
    10621 ccttactggc ggcttaccag tggcttaccg cccggagatg ccgagcgaga cggacgcttc
    10681 gcgtacaatc gccgccccat gtcgaatcgc agccgccgag aggcggaagc tcgagccgag
    10741 gcgcgccgcc gcgcgcgtta ccaggcgcgt ggcgtgccgg tcgaggagcc ggacgaggag
    10801 gcctcggagg ccgacgagcg ccgggaaagc cgggcgccgg ccgcgggggg aggcttcctg
    10861 acccgccttt tcccgccggc gccgccgctc cccaaccgcc ccgacccgct gcgcaacttc
    10921 agctactcgg ggccgcttcg cggtgtcgtc gcgggcctgt acctgctcat gcg
//
LOCUS       DATACA010000200         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2756932, whole genome shotgun sequence.
ACCESSION   DATACA010000200 DATACA010000000
VERSION     DATACA010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2756932"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2051)
                     /locus_tag="VHK28_04125"
     CDS             complement(<1..>2051)
                     /locus_tag="VHK28_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX2141450.1"
                     /translation="DAAGFHVFRAVLEPADDHFVENNAADAYVLVSGEPQVLLATDDS
                     GRAAELLDALEEARHNVTVVPAGGVPSSLATLAGYDAILLDNVSAAELGPTTMASLQV
                     YVRDLGKGLVMLGGRDSYGAGGYLNTAMEEILPVYMTVRDRERSPDVALVAVVDKSGS
                     MADCHCTGDERDSATSGTRGFEKVDIAKEAILRAADALAPTDQLGVVAFDEGAHWALR
                     TGPIDVNGLQSSLGFRADGQTNIFAGLKAAYDDLVRNPAKLRHIILITDGWSQSGAYD
                     QLLADMKAAGITLSTIGTGGGSADILRRLAEQSGGRYYDAGDATTIPDIFLRETIRTA
                     GEQIVEEAFQPIPSAPSEILRGLDAGRLPALRGYNATTPKGTATVALLTGREDPLLAQ
                     WQYGLGRAAAWTSDARQQWAGEWIGTPEYGTLLSQLVAWTLPPQDDQGIDVRFSPGRE
                     GQLNVDVTSLDPDGAPRNFYRSVLRLVGPGLEPAQVVLEQVGPGHYAGSVRAEEPGAY
                     LVRVAQTSPGAEAASRTLGMVSPAAEEFRRLGVDGEALAELAAAGGGRELVVDEEGQA
                     AAAVWSHDISAAAFPTPVWPFLLLLAMVLVPLDVGVRRVALSRADLRRARQWSARRVG
                     LARSDPEAVPGLAELQAARDRTRRRADRAPRGAEPVAPRPVPDAAIAAPAAAPSDAPE
                     S"
BASE COUNT          270 a          762 c          732 g          287 t
ORIGIN      
        1 ggactcgggc gcatcagacg gcgcggctgc cggcgcggct atcgctgcgt cgggcacggg
       61 tcgcggagcc accggttccg ctcccctggg agcacggtcg gcccggcgcc gcgtccggtc
      121 ccgagcggcc tggagctcgg ccaacccggg cactgcctcc ggatcgctcc gcgccagccc
      181 gacgcggcgt gccgaccact gccgcgcccg tcgcaggtcg gcgcggctga gcgccacgcg
      241 gcgcacgccg acgtccaatg gcacgaggac catggccagc agcagcagga acggccacac
      301 cggtgttggg aaggcggcgg ccgagatgtc atgggaccag accgcggctg ccgcctggcc
      361 ctcctcgtcc accaccagct cgcgtccgcc cccagcggcc gccagctcgg cgagtgcctc
      421 gccgtcgacg cccaggcgcc ggaactcctc cgccgccgga ctcaccatgc ccagggttcg
      481 tgatgccgcc tccgcgccgg ggctggtctg agccacgcgc accaggtagg cgccgggctc
      541 ctcggcccgc acgctgccgg catagtggcc ggggccaacc tgctccagca cgacctgcgc
      601 gggctccagc ccggggccga ccagccgcag cacgctgcgg tagaagttgc gcggcgcgcc
      661 gtccggatcc agggaggtca cgtcgacgtt gagctgtcct tcgcgacccg gcgagaaccg
      721 cacgtcgatg ccctggtcat cctgcggcgg cagtgtccag gccaccagct gcgagagcag
      781 cgtgccgtac tcgggggtgc caatccactc gccggcccac tgctgccgcg cgtcgctggt
      841 ccaggccgca gcgcggccca gcccgtactg ccactgggca agcaggggat cctcgcggcc
      901 ggtgagcagc gccaccgtgg cggtgccctt tggcgtagtg gcgttgtagc ctcgcagcgc
      961 cgggagccgc ccggcatcca gcccgcgcag gatctcggac ggggccgacg ggatcggctg
     1021 gaaggcctcc tccacgatct gctcccccgc ggtgcggatc gtctcgcgca ggaagatgtc
     1081 cgggatggtg gtcgcatcgc cggcgtcgta gtagcggccg cccgactgct cggccaggcg
     1141 gcgcaggatg tcggccgacc cgccaccggt gccgatggtc gacagggtga tgccggccgc
     1201 cttcatgtcg gccagcagct ggtcgtaggc gccggactgc gaccagccat cggtgatcag
     1261 gatgatgtgg cgcagcttgg cggggtttcg gaccaggtcg tcgtaggccg ccttcaggcc
     1321 ggcgaatatg ttggtctggc cgtccgcccg gaagccgagc gacgactgga ggccgttgac
     1381 gtcgatcggt ccggtgcgca gcgcccagtg cgcgccctcg tcgaaggcga ccacgcccag
     1441 ctggtcagtg ggcgccagcg catcggcggc gcgcaggatg gcctccttgg caatgtccac
     1501 cttctcgaag ccgcgcgtcc cgctggtggc gctatcccgc tcgtcgccgg tgcagtggca
     1561 gtccgccatg ctgccgctct tgtcgaccac ggccaccagg gccacgtccg gggagcgctc
     1621 ccggtcgcgg accgtcatgt agaccgggag gatctcctcc atggccgtgt tcaggtagcc
     1681 gccggccccg tagctgtcgc gcccgccgag catgaccagc cccttgccca ggtcgcgcac
     1741 gtagacctgc aggctggcca tggtggtcgg tcccagctcc gccgcggaga cgttgtccag
     1801 caggatggcg tcgtagccgg ccagcgtcgc cagcgacgac ggaacgccgc cggcgggcac
     1861 cacggtcacg ttgtgccgcg cctcctccag tgcgtcgagc agctccgccg ctcgcccgga
     1921 atcgtcggtg gccagcagca cctgcggctc gccgctgacc agcacgtagg catcggccgc
     1981 gttgttttcc acgaagtggt catccgcggg ctccaggacg gcgcggaaga cgtggaagcc
     2041 ggccgcatcg g
//
LOCUS       DATACA010000201         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1954309, whole genome shotgun sequence.
ACCESSION   DATACA010000201 DATACA010000000
VERSION     DATACA010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954309"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VHK28_04130"
     CDS             <1..946
                     /locus_tag="VHK28_04130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2141451.1"
                     /translation="HSRIPSLPGLDEVGAWTNREGTSLRELPDGVVVLGGGPSGLELG
                     QVYARYGVPVTLVHPRDRVNDRDHPRSSRLLAEGLERDGVDIRLNARAEAVRAHAGSN
                     GRHVVELSDGSTAEGEAILLTIGREIPLDTLNLPAIGVEARDGRVDPDERLRIAPNVY
                     VVGDIAGPELHTHLAHYQGEAAARLAAGQEVSPDFTAIPRATYTDPETSSVGLLVEQA
                     RERGIDAVEYSQDLAKTAKGYTSERDGHVTIVVDRERRTLVGAFMTGAGVSETIHEAV
                     LAVKLGTPLSVLADTIHAFPTVARVLGSLFVTAWKEIG"
     gene            973..1710
                     /locus_tag="VHK28_04135"
     CDS             973..1710
                     /locus_tag="VHK28_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2141452.1"
                     /translation="MDLTLALALLFFAAAVLYGMVGHAGASAYLAAMALLGVAPEVMR
                     PTALVLNILVAGIVTARFARAGFVRPASALPFLVTSVPAAFVGGTIDLPGDVYRPLVG
                     ATLLFAAVRFGITAARPAEERVTRAPLPAALVSGALIGLLAGLTGTGGGIVLTPLLIA
                     AGWAGTRFAAGTSVIFILANSVAGMSGNLASVGQLPSALPLWLGAVAVGGLVGSELGS
                     RRLPAPWVRRALALVLALAGLKLILLP"
     gene            complement(1884..>2513)
                     /locus_tag="VHK28_04140"
     CDS             complement(1884..>2513)
                     /locus_tag="VHK28_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2141453.1"
                     /translation="AELMFEDGIGLVVRHAGLSHPFSRPHPAYLLVEVDGASDPTDEL
                     AAAIEAGGALVHDAVLATDEAQRARLWQLRERHTEAVNAAGVPHKLDVAVPVGQLGEF
                     ANRVRAVISGVAPDASVYLYGHVGDGNLHVNVVGPAPEDEAVDAAVLELAIAFGGTIS
                     AEHGIGVAKTRWLEADRGPADVAAMRAIKQALDPGGVLNPGVLLPLAPS"
BASE COUNT          315 a          926 c          893 g          379 t
ORIGIN      
        1 gcactcgcgt atcccatcgc tgcccgggct cgatgaggtc ggagcctgga cgaaccgcga
       61 gggcacctcg ctccgcgagc tcccggacgg ggtggtcgtg ctgggcgggg gtccgagcgg
      121 gctggagctc ggccaggtgt atgcccgcta tggcgtaccg gtcaccctgg tccacccccg
      181 ggaccgcgtg aacgatcgcg atcacccgcg ctcctcgcgc ctgctggccg agggcctgga
      241 acgggacggc gtcgacatcc ggctcaatgc gcgagcggag gcggtccgtg cgcacgcggg
      301 ctcgaatggg cgacatgtcg tggagctgtc cgacggcagc acggccgagg gagaggcgat
      361 cctgctcacc atcgggcgcg agatcccgct ggacaccctc aacctccctg cgatcggagt
      421 ggaggcgcgc gacgggcggg tcgacccgga cgagcgcctg cggatcgcgc cgaacgtgta
      481 cgtggtcggg gacatcgccg gaccggagct tcacactcac ctggcccact accagggaga
      541 ggccgcggcg cggctcgccg ccgggcagga ggtctcgccc gatttcacgg ccatcccgcg
      601 cgcgacctac accgatcccg agacctcgtc ggtcggcctg ctggtggagc aggcacggga
      661 gcgcggcatc gacgccgtcg agtacagcca ggacctggcg aagaccgcca agggctacac
      721 ctcggagcgg gatggccacg tcaccatcgt ggtcgaccgc gagcggcgca ccctggtcgg
      781 cgccttcatg accggggctg gcgtcagtga gacgatccac gaggcggtgc tggcggtgaa
      841 gctcggcacg ccactctccg tgctggccga caccatccac gccttcccca ccgtcgcccg
      901 cgtactcggc tcgctgttcg tgaccgcctg gaaggagatc ggctgagcgg ccgccgctag
      961 catcgggccc tcatggacct gacgctggcc ctggcgctcc tgttcttcgc cgccgccgtg
     1021 ctgtacggca tggtgggcca cgccggcgca tcggcctacc tggcggccat ggcgctgctg
     1081 ggggtcgcgc cggaggtgat gcgcccgacc gccctggtgc tcaacatcct ggtggccggg
     1141 atcgtgaccg cccggttcgc gcgagccggg ttcgtgcgac cggcatctgc gctcccgttc
     1201 ctggtcacct ccgtgccggc ggcgttcgtc ggtggcacga tcgacctgcc cggcgacgtc
     1261 taccggccgt tggtcggcgc caccctgctc ttcgcggcgg tccgcttcgg catcaccgcg
     1321 gcccgacccg cggaggagcg cgtgacgcga gcccccctgc ccgcggcgct ggtcagtggg
     1381 gcgctgatcg ggctgctggc cggcttgacc ggcaccggcg gcgggatcgt cctcacgccg
     1441 ctgctgatcg ccgccggctg ggccgggacg cgcttcgcgg ccgggacgtc cgtgatcttc
     1501 atccttgcca actcggtcgc cgggatgtca gggaacctgg cgtccgtcgg ccagctgccc
     1561 tccgcgctgc ccctgtggct gggggcggtg gcggtcggcg ggctggtcgg gtcggagctc
     1621 ggcagccggc ggctgccggc accctgggta cggcgtgcgc tggcgttggt gctggcctta
     1681 gcggggctga agctcatcct cctcccctga cccgccgaca tatcgggcag aaattcacgt
     1741 gacccgggtg ccgcgtgtcg ttctgcccat gtgccaggca ggcctcagcg tgaggggcga
     1801 agttcttgtc gcctcgtgct gcgggctgcg cgatgtatca gcgaactgac acctggcggc
     1861 tgcccgctgt gaagaactgc ccgctacgac ggcgccagcg gcagcagcac gcccgggttc
     1921 agcacaccac cggggtcgag cgcctgcttg atggcgcgca tggcagccac gtcggcgggt
     1981 ccgcggtcgg cctccagcca gcgcgtcttg gccacgccga tcccgtgctc ggcgctgatg
     2041 gtcccgccga acgcgatcgc gagctccagg accgcggcgt ccaccgcctc gtcctccggg
     2101 gccggcccga ccacgttgac gtgcaggttc ccgtcaccca cgtgcccgta caggtagacc
     2161 gatgcgtccg gcgccacccc gctgatcacg gcgcgcacgc ggttggcgaa ctcacccagt
     2221 tgtcccaccg gcaccgccac gtccagcttg tgcggcaccc cggcggcgtt gaccgcctcg
     2281 gtgtgccgtt cgcgcagctg ccacagccgc gcccgctgcg cctcgtcggt ggccagcacc
     2341 gcgtcgtgca ccagggcgcc gccggcctcg atggctgccg ccagctcatc ggtcgggtcc
     2401 gacgcgccgt cgacctccac cagcaggtac gccgggtgcg gacgcgagaa cggatgcgac
     2461 agtccggcgt ggcgcaccac cagcccgatc ccgtcctcga acatcagctc ggc
//
LOCUS       DATACA010000202        10517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_107012, whole genome shotgun sequence.
ACCESSION   DATACA010000202 DATACA010000000
VERSION     DATACA010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107012"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2588
                     /locus_tag="VHK28_04145"
     CDS             303..2588
                     /locus_tag="VHK28_04145"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX2141454.1"
                     /translation="MTEAAATQTPSFEPAELSLPVVGMTCASCVNRVERFLNRADGVE
                     QAVVNLASERASVRYDPRRIDPAGIAEAIRASGYEVGREAAAEVGTAEEEVAEERQRA
                     AERRELLWSAVAATGIGLAMMAVAMWPGGPPWPMERINVWLLAPATFVQFVLGRRFLV
                     RAARGLRHGDVTMDTLVAMGTLAAYGYSLVLTLLPEAVMAAGLSLETYYDSAAIIIGL
                     VLLGRWLEARARGQAAGAVAALLRLRPTTARVMRDGREVDLPLDQVRVGDLLRVRPGE
                     RVPVDGVLLEGASAVDESMLTGESIPVEKAPGEAVIGAAMNTSGSFVMRAERVGRDTV
                     LGQIVRLVEQAQGSKAPIQRVADAVTGWFVPAVLLIAAVTFVTWLLAGPDPALPLALS
                     AAIAVLIIACPCAMGLATPTAIMVGTGKGAEVGILVRDAAALEAAGRLDAVVLDKTGT
                     VTRGQPELVGIAPLADWTDAGLLGMAASAERGSEHPLAEAVVRAAGAAGLDLASASGF
                     ESEPGGGVRARVDDRSVLVGSERLLRDAGVDVDCMLEAAAASAAAGQTPVLVAVDGAP
                     AGVLSIADEVRPESGEAVRRLARAGLEVWLVTGDRAAVGAAVGQQIGIPPERILAEVR
                     PEEKAARVAALQSEGRRVAMVGDGINDAPALAQADVGIAIGTGADVALEASDLTILGD
                     DLRAVPGAIGLSRATMRTIRQNLGWAFGYNVLLIPVAAGMLFPITGWLLSPALAAGAM
                     ALSSVSVVANSLRLRRFRATT"
     gene            2608..2817
                     /locus_tag="VHK28_04150"
     CDS             2608..2817
                     /locus_tag="VHK28_04150"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX2141455.1"
                     /translation="MDHQHHDSTPGTTAKDPVCGMDVNPEVASAAGLTADYEGSTYYF
                     CGRGCMLDFGDEPRRFFDPDYVPHM"
     gene            2872..3798
                     /locus_tag="VHK28_04155"
     CDS             2872..3798
                     /locus_tag="VHK28_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141456.1"
                     /translation="MPLIEARSLTKRYGSHVTALDGLTLEVEPGIIGLVGANGAGKST
                     FLKILLGLLEPTSGSALVMGRDVISEGTELRQFVGYLPEHDCLPPDVSATEFVTHMGR
                     MSGLPAGASRERTAEVLRHVGLYEERYRPIGGYSTGMKQRVKLAQALVHDPKLLLLDE
                     PTNGLDPAGRDEMLELIRRTGTEFGIATVVASHLLGEIERVCTFLLAIDAGKLLRAAP
                     LASFTERTGMLGVEVEEGAPALAERLATAGLTISVEGRTVLVDAPDERPYDIVRDAVD
                     DLGLALVRLEHRRHRLEDLFRGDDGKEGTDAA"
     gene            3788..4738
                     /locus_tag="VHK28_04160"
     CDS             3788..4738
                     /locus_tag="VHK28_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141457.1"
                     /translation="MPPEATGPGAAAGSDAPLQQPAAGAIYDLGYRGYDGERLGRTAA
                     VTTLFWSSLRAAFGLGRSGRAKIIPWGLTAFLLAPAAISVAISALVPVGEANPFTYDN
                     YLFGSASIYALFVAAQAPELVSGDQRHRVLSLYFSHALLRSDYALAKLGALAAALFLI
                     GFAPMLLLFLGSVLVAADVAGAFGDQLENLPQVFLAPAIFALPLVALGMAIAAWTPRR
                     AYATGAIIAVFIVTAAVSGIFDQAFSGGDTGGFVAEWSQLLNPFVAAEGTRDLLVGGT
                     NPEAPIDRTNLPLWVFAAEWALIVAAGTASTLWRYRRIAA"
     gene            4735..5682
                     /locus_tag="VHK28_04165"
     CDS             4735..5682
                     /locus_tag="VHK28_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141458.1"
                     /translation="MTPAPDAATKAAAVELAGASRWYGNVVAVNDVSFRLEGGVTGLL
                     GPNGAGKSTILHMIAGFLAPSAGTVTIAGSPSRGHPEMYRQLGLVPEHEAVYPFLTGR
                     QFVAANARLQGLAPATAAEAAARAIGMVELEGAQDRAIGTYSKGMRQRAKVAASLVHD
                     PPILLLDEPFNGMDPRQRLHMMELLRRMGSEGRTILFSSHILEEVERLAERVLVIVAG
                     RLAASGNFREIRRLMIDRPHTFTVRSSDDRVLAAALLHQPAVAGVALDGGLITVRTSD
                     FGEFSRTIGSVARRANISLFEVLPTDESLESVFSYLVRR"
     gene            5679..6413
                     /locus_tag="VHK28_04170"
     CDS             5679..6413
                     /locus_tag="VHK28_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141459.1"
                     /translation="MNTTIAAITLRQLLSRRRVILLLLLAAVMVVVALVLRLSLDDVY
                     VEEYTAALLSNLGVATLMPLVALLFGSGALGSELEDGTAVFLLAKPISRTTILLTKLA
                     VAAACAVALTAIPMLLAGLIGAAGVDVGLAIAFCLASAVGAVVYTAVFIALSLITSRA
                     LVVGLGYVLIWEGLLAALFAGTRTFSVRQHVLAIADALTDAPQSVFTAQLELPTALVV
                     GGLVLVGATVLAARRLATIEIAGENA"
     gene            6488..7498
                     /locus_tag="VHK28_04175"
     CDS             6488..7498
                     /locus_tag="VHK28_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2141460.1"
                     /translation="MVEHVLDSQVLTGNPLGDPHRRPLLVQVPPGYDDEPDVRYPTIY
                     VLQGLTGQVDQWLGRRPSAQPNIPERMDALFASGQSPAAILVYVDAWTSLGGSQFVDS
                     PGTGRYHTYLCHEVVPFVDGEYRTLASAAHRAVTGHSSGGYGAMVNAMLRPDLFGAFA
                     THAGDALFELCFLRDFGKAARTLRDRYDGSYERFWAEYRSRGRSVPGDGELLNLWCMA
                     ACFSTDADGTVRLPFDTGTGRLVSDVWDRWLARDPVRMAPERADALGGMHAIWIDAGR
                     GDEYFLDLGAEAFRRELEQLGIGEPTVRFELHEGGHGGAEWRYPLAVAWLAERLAPAV
                     PG"
     gene            complement(7495..9474)
                     /locus_tag="VHK28_04180"
     CDS             complement(7495..9474)
                     /locus_tag="VHK28_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2141461.1"
                     /translation="MADTDGGRLGQVVASALDAVVTMDVEGTIVGWNPRAEEVFGWRA
                     NDVLGLPMDQVILPARYRRAHRAGLARYLRSGETRILNRRVEVEALQRSGREIPVELA
                     VTALPRKGRVEFCAFIRDLSRERATEAALRETEERFRLLVETLPGIVYIDDPGARGIY
                     ISPQIQSLLGYSAEEWLLNPDRWVDALHPDDRERAVAELSAGEAGEQRFTSEYRLITR
                     DGGAVWIRDEATPVRDSRGRILIHGVMFDVTREREIEAELAAEVAERAEIATALSSFR
                     PGSDAHATADALLRELLRIRDIDIGVIYAFDGAAATPLAVSAPPGAPVAVGRPLPTHR
                     TAYLQANALRGPWVDEWREAPDADDYVRAWLEVGLRIGAYVPLSSDGAPWGLIAAGTT
                     TEMPGQRLARSLPALLDFATVGNAVLGPQVHARRAESEVRALRELIDTSALRSVFQPI
                     VHLESGEVIGYEALTRFTDGSLPLQRFAEAERAGMAIELEAACMASAIAAARHLPSGP
                     WLSVNLSPIALPGLRQLAAVAAGADRPLVVEITERSAIDDYAAARRLLRRHLPQARVA
                     VDDAGAGFASLRHIAELRPSIVKLDMQLVRNVHRDPAREALVAGMVHFASASGCELVA
                     EGIETEAERRALLRLGVMIGQGFLLGRPDAVVDAA"
     gene            complement(9510..>10517)
                     /locus_tag="VHK28_04185"
     CDS             complement(9510..>10517)
                     /locus_tag="VHK28_04185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX2141462.1"
                     /translation="AAVFRSLHEFTQAVSGTLDRDRLAVAMVDAARRVVPADVVVLTV
                     LEQQTGRYYVRAITDGDAAWLGVEIRPGQSLAGRAIRDRNLVIDADLERSALPERYRD
                     VPAPDRMLGAAIPLVRDGVVVGALSLLRVDRTDAFRPIEREAMELLAGHAALTLANAF
                     LHAEVEQLAIRDPLTGLHNRRYFDEAADRMIASWHRTEPALRRPVSAILFDLDHFGDF
                     NKHHGHLVGDHVLRSFARILDDRFRAADLVARLGGEEFIVVLEGAGREDAERIAEQVR
                     VALASLDLVDEDGRRLTVTVSAGCTQLEESAATREQLLRTADMAMFMAKRAGRDRVVA
                     A"
BASE COUNT         1407 a         3752 c         3807 g         1551 t
ORIGIN      
        1 gccggctgaa gggcaccggt tccaggccga cggaggcggc cacgtcctgc aggagctgga
       61 gcgtcggata gtcgtcctcg accaggaagg cacgctgagg aggccgccct cggcggcccc
      121 ggtggtgtgg ttcctgattc atggcgtaca tccgtccccc tggaggaccg gttgccgtcg
      181 aggcgaaccg ggtttcgcct ggactaggag cacgatgcat gccactggct acccggttga
      241 agcggaagcc gctcgccggc gttgttgtcg cctatactcc ccgagagtat gctgcttaca
      301 ccgtgacgga agccgcagcc acacagaccc cctccttcga gccagccgag ctcagcctgc
      361 cggtggtcgg catgacctgt gcctcgtgcg tgaaccgcgt cgagcgcttc ctgaaccgtg
      421 ccgatggcgt cgagcaggcg gtcgtcaacc tggccagcga gcgagcctca gtgcgctacg
      481 acccgcgccg catcgacccc gccggcatcg cggaggccat ccgggcctct ggctacgagg
      541 tcggtcgcga ggcggccgcc gaagtcggga ccgccgagga ggaggtggcc gaggagcgcc
      601 agcgggccgc ggagcgccgc gagctgctgt ggagcgcggt ggccgccacc ggcatcggcc
      661 tggccatgat ggccgtcgcc atgtggcccg gcgggccgcc ctggcccatg gagcgcatca
      721 acgtctggct gctggcgccg gccaccttcg tccagttcgt gctggggcgc cgcttcctgg
      781 tgcgtgccgc ccgcgggctg cgccatggcg acgtgaccat ggacacgctg gtggccatgg
      841 gcaccttggc cgcctacggc tactccctgg tgctcacgct gcttcctgaa gcggtcatgg
      901 ccgccggcct ctcgctggag acgtactacg acagcgcagc catcatcatc ggcctggtcc
      961 tgctcgggcg ctggctggag gcccgggccc ggggccaggc cgccggcgcg gtggctgccc
     1021 tgctgcggct gcggcccacc acggcacgcg tgatgcgcga cgggcgcgag gtcgacctgc
     1081 cgctggacca ggtgcgcgtc ggcgacctgc tgcgggtgcg tcccggggag cgggttcccg
     1141 tggacggcgt gctgctggag ggcgcgtcgg ccgtcgacga gtcgatgctg accggcgagt
     1201 cgatcccggt cgagaaggcg cccggcgaag cggtcatcgg cgccgccatg aacacctccg
     1261 gctcattcgt gatgcgggcc gagcgcgtcg gccgcgacac cgtgctcggg cagatcgtgc
     1321 gcctggtcga gcaggcgcag ggctccaagg cacccatcca gcgggtggcg gacgcggtca
     1381 ccggctggtt cgtgcccgcg gtgctcctga tcgcggccgt cacctttgtc acctggctgc
     1441 tggccggccc ggacccggcc ttgccgctgg ccctttcggc ggcgatcgcg gtgctgatca
     1501 tcgcctgccc gtgcgccatg ggcctggcca cgccgaccgc gatcatggtg ggcaccggca
     1561 agggcgccga ggtgggcatc ctggtgcgcg acgcggcggc gctggaggcc gcgggtcggc
     1621 tcgacgcggt ggtgctggac aagaccggca ccgtcacccg cggccagccc gagctggtcg
     1681 gcatcgctcc cctcgcggac tggaccgatg ccggcctcct cggcatggcg gccagcgccg
     1741 agcgcggcag cgaacatccc cttgccgagg cggtggtccg agcggccggg gcagctgggc
     1801 tcgacctggc atcggcgagt gggttcgagt cggagccggg cggcggcgtg cgcgccaggg
     1861 tcgacgatcg ttcggtgctg gtcggcagcg agcggctgct gcgggacgcc ggcgtagacg
     1921 tcgactgcat gcttgaagcc gcggcggcat ccgctgccgc gggacaaacg ccggtgctgg
     1981 tagccgtgga cggcgcgccg gcaggcgtgc tgtccatcgc cgatgaggtg cgtcccgaat
     2041 ctggcgaggc ggtgcggcgg ctggcgcgtg ccggcctgga ggtctggctg gtgaccggcg
     2101 accgtgccgc ggtgggggcg gcggtgggac agcagatcgg tattccgccg gagcgcatcc
     2161 tggccgaggt gcgtcccgag gagaaggccg cccgggtggc tgcgctgcag tcggagggcc
     2221 ggcgggtggc catggtcggc gacggcatca atgacgcacc ggcgctggcg caggcggacg
     2281 tcggcatcgc cattggcacc ggcgccgacg tggcgctgga ggcatcggac ctcaccatcc
     2341 tgggggacga cctgcgcgcc gtgccggggg ccatcggtct gtcgcgcgcg accatgcgca
     2401 ccatccgtca gaacctgggc tgggcgttcg gctacaacgt gctgctcatc ccggtggccg
     2461 ccggcatgct gttcccgatc accgggtggc tgctgtcgcc ggcgctggcg gccggggcca
     2521 tggccctgtc cagcgtgagc gtggtggcca actcgctgcg gctgcgacgc ttccgtgcca
     2581 ccacctgata gccccatgag gagaccgatg gatcaccagc atcacgacag cacccccggc
     2641 accacggcca aggacccggt gtgcggcatg gatgtcaacc cggaagtcgc atccgcggcg
     2701 ggcctgaccg cggattacga gggcagcacc tactacttct gcgggcgtgg ctgcatgctc
     2761 gacttcggtg acgagccgcg gcgattcttc gacccggact acgtcccgca catgtagcgg
     2821 tcagacgctc gcgccggcgg tcgcgggtca acgcgcgcta gactcccgca gatgccgttg
     2881 atcgaggccc gctccctcac caagcgctac ggctctcatg tcaccgcgct ggacgggctg
     2941 acgctggagg tcgagcccgg gatcatcggg ctggtcggag ccaacggcgc ggggaagagc
     3001 accttcctca agatcctgct ggggttgctg gagcccacca gcggatcggc cctggtgatg
     3061 ggacgcgacg tcatcagcga gggcacggag ctccggcagt tcgtcggcta cctgcccgag
     3121 cacgactgcc tgccgccgga cgtgtcggcc accgagttcg tgactcacat gggccgcatg
     3181 tccgggctgc cggccggcgc ctcccgcgag cgcaccgccg aggtgctgcg gcacgtcggc
     3241 ctctacgagg agcgttaccg ccccatcggt ggctactcca ccggcatgaa gcagcgcgtc
     3301 aagctggcgc aggcgctggt ccacgacccc aagctgctgc tgctcgacga gccgaccaac
     3361 ggcctggatc ccgcgggtcg cgacgagatg ctggagctca tccggcgcac cgggacggag
     3421 ttcggtatcg ccaccgtggt ggccagccat ttgctgggcg agatcgaacg ggtctgcacc
     3481 ttcctgctgg cgatcgacgc cggcaagctg ctgcgggcgg cgccgctggc gtcgttcacc
     3541 gagcggaccg gcatgctcgg cgtggaggtg gaggagggcg cgccggcgct ggccgagcga
     3601 ctggccacgg ccgggctgac gatcagcgtc gagggccgca ccgtgctggt ggacgcgccg
     3661 gacgagcgcc cctacgacat cgtgcgcgac gccgtggacg acctgggcct ggcgctcgtg
     3721 cgcctcgagc accggcggca ccggctggag gacctcttcc gaggtgacga cggcaaggag
     3781 ggcacggatg ccgcctgagg ccacaggccc cggcgcggcg gccgggtccg atgctcccct
     3841 gcagcagcct gccgccggcg cgatctacga cctcggctac cgcggctacg acggggagcg
     3901 cctggggcgc accgccgcgg tcaccacgct cttctggtcg agcctgcgcg ccgcgttcgg
     3961 cctgggtcgg agcgggcgag ccaagatcat cccctggggc ctgaccgcgt tcctgctggc
     4021 gccggccgcc atctcggtcg ccatctcggc gctcgtgccg gtcggcgagg ccaacccatt
     4081 cacctacgac aactacctgt tcgggtcggc cagcatctac gccctgttcg tggccgccca
     4141 ggcgcccgag ctggtgagcg gcgatcagcg gcaccgagtg ctttccctct acttcagcca
     4201 cgctctgcta cgcagcgact acgcgctggc caagctgggt gcgttggcgg ccgccctgtt
     4261 cctgatcgga ttcgcgccaa tgctgctgct cttcctggga tcggtgctgg tggcggccga
     4321 cgtggccggt gccttcggcg atcagctgga gaacctgccc caggtgttcc tggcgcccgc
     4381 catcttcgcc ctgccgctgg tggcgctggg catggcgatc gcggcctgga cgccgcggcg
     4441 tgcgtacgcg accggcgcca tcatcgcggt cttcatcgtg accgcggccg tgtccggcat
     4501 cttcgaccag gccttcagcg gcggggacac cgggggcttc gtggcggagt ggtcgcagct
     4561 gctcaacccg ttcgtcgccg cagaaggaac gcgtgacctg ctggtgggcg gcaccaaccc
     4621 cgaagcgccg atcgatcgca ccaacctgcc gctctgggtc ttcgccgccg agtgggcgct
     4681 cattgtggcc gccggcacgg cctccaccct gtggcgctac cggaggatcg ccgcatgacg
     4741 cccgcacccg acgcggcgac caaggcggcg gccgtcgagc tggccggcgc ctcgcgctgg
     4801 tacggcaacg tcgtggccgt taacgacgtc agcttccgtc tggagggcgg cgtgaccggg
     4861 ctgctcggac cgaacggagc gggcaagagc accatcctgc acatgatcgc cggcttcctg
     4921 gctccgtcgg ccgggacggt gaccatcgcc ggctcgccgt cgcgcggcca tccggagatg
     4981 taccgccagc tcggtctggt gccggagcac gaggccgtct acccgttcct caccggccgg
     5041 cagttcgtgg cggccaacgc acggctgcag ggcctggccc cggccacggc ggccgaggcg
     5101 gcggcccggg ccatcggcat ggtggagctg gagggcgcgc aggatcgcgc catcggcacc
     5161 tactcgaagg gaatgcggca gcgggcgaag gtggctgcgt ccctggtgca tgacccgccc
     5221 atcctgctcc tggacgagcc gttcaacggc atggaccccc gacagcgcct gcacatgatg
     5281 gagctcctgc gacggatggg ctccgagggc cgaaccatcc tgttctcgtc gcacatcctg
     5341 gaggaggtgg agcggctggc cgagcgcgtg ctggtcatcg tggccggccg gctagcggcc
     5401 tcgggtaact tccgtgagat ccggcggctg atgatcgacc ggccgcacac cttcaccgtc
     5461 cgctcgtcgg acgaccgggt gctggccgcc gcgctcctcc atcagcccgc ggtggccggg
     5521 gtggcgctgg atggtggcct gatcacggtc cgcacctcgg acttcggcga gttcagccgc
     5581 accatcggca gcgtggctcg gcgggccaac atctcgctgt ttgaggtgct gcccaccgac
     5641 gagtcgctcg aatcggtctt cagctacctg gtgcgccggt gaacaccacc atcgccgcga
     5701 tcaccctgcg ccagctgctg agccggcgac gcgtcatcct gctgctgctg ctggcagccg
     5761 tgatggtggt ggtcgcgctg gtgctgcgcc tcagcctcga cgacgtgtac gtggaggagt
     5821 acaccgccgc gctgctctcc aacctcggcg tggcgacgct gatgccgttg gtggccctgc
     5881 tgttcggcag cggcgcgctg gggagcgagc ttgaggacgg gacagccgtc ttcctccttg
     5941 ccaagcccat cagccgcacg accatcctcc tcaccaagct ggcggtggcg gcggcctgcg
     6001 cggtggcgct gaccgcgata cccatgctgc tcgccggcct gatcggcgcc gccggagtcg
     6061 acgtcggcct ggcgatcgcc ttctgcctcg cctccgccgt gggcgcggtg gtctacaccg
     6121 ccgtgttcat cgccctgagc ctgatcacca gccgcgcgct ggtggtcggc ctgggttacg
     6181 tgctcatctg ggaggggctg ctggccgccc tcttcgccgg gacgcgcacc ttcagcgtcc
     6241 ggcagcacgt cctggccatc gccgacgctc ttaccgatgc gccgcagtcg gtgttcaccg
     6301 cccagctcga gctgcccacc gcgctggtgg tgggaggcct ggtgctggtc ggcgccacgg
     6361 tgctcgccgc ccggcggctg gccacgatcg agatcgccgg ggagaatgcg tgacccagcg
     6421 cccgggcgtg cgcagcacgc tgttgcgcga ggccggtcct ccgtgggcgt accccttcgc
     6481 cggcggcatg gtggaacacg tcctcgacag ccaggtgctc accggaaatc cgctgggcga
     6541 cccccaccgg cggccgttgc tcgtccaggt gccgcctggc tatgacgacg agccggacgt
     6601 ccgctacccg acgatctacg tcctccaagg gctgaccggc caggtcgacc agtggctcgg
     6661 gcgccgccca tcggcgcagc caaacatccc ggagcgcatg gacgccctgt tcgccagcgg
     6721 gcagtcgccg gcagccatcc tggtctatgt cgatgcctgg accagcctgg gcggcagcca
     6781 gttcgtcgac tcgccgggga ccggccgcta ccacacctac ctgtgccacg aggtggtccc
     6841 gttcgtggac ggtgagtacc gcaccctggc ctcggccgcc caccgcgccg tgacggggca
     6901 cagcagcggc ggctacggcg ccatggtcaa cgccatgctt cgccccgacc tgttcggcgc
     6961 ctttgccacc cacgccggcg acgcgctgtt cgagctgtgc ttcctgcgcg acttcggcaa
     7021 ggccgcccga acgctgcggg atcgctacga cggcagctac gagcggttct gggcggagta
     7081 ccgatcgcgt gggcgttccg tccccggcga cggcgagctc ctgaacctgt ggtgcatggc
     7141 cgcctgcttc tcgaccgatg cggacggcac cgtccgcctg cccttcgaca ccggcaccgg
     7201 ccgcctggtc tcggacgtct gggaccgatg gctggcccgt gacccggtcc gcatggcgcc
     7261 ggaacgagcc gatgcgctgg gcggcatgca cgccatctgg atcgatgccg ggcgcggcga
     7321 cgagtacttc ctcgacctcg gcgcggaggc cttccgccgc gagctggagc agctgggaat
     7381 cggcgagccg acggtgcgct tcgagctgca cgagggcggc cacggcggcg ccgagtggcg
     7441 ctatccgctg gcggtcgcct ggctggccga gcggctggcg cctgcggtcc cgggctaagc
     7501 ggcgtccacc accgcgtcgg gccgtccgag caggaacccc tggccgatca tcacccccag
     7561 gcgcagcagc gcccgacgct cggcctcggt ctcgatgccc tccgccacca gctcgcagcc
     7621 cgaggcgctc gcgaagtgga ccatgccggc caccaacgcc tcgcgggcag ggtcgcggtg
     7681 aacgttgcgc acaagttgca tgtccagctt cacgatggac gggcggagct cggcgatgtg
     7741 ccgcaggctg gcgaagccgg cgcccgcgtc gtcgaccgcg accctggcct gcggcaggtg
     7801 gcgccgcagc agtcgacggg cagcagcgta gtcgtcgatc gccgatcgct cggtgatctc
     7861 gaccaccagc ggacggtcgg cgccggccgc gaccgcggcc agctggcgca gcccgggcaa
     7921 ggcaatcggt gagaggttca cgctgagcca gggaccggac ggcaggtgcc gggcggcggc
     7981 gatggcggag gccatgcagg cggcctccag ctcgatggcc atgccagcgc gctcggcctc
     8041 ggcgaagcgc tggagcggca ggctgccatc ggtgaagcgg gtcagcgcct cgtagccgat
     8101 cacctcgccg ctctccaggt gcacgatggg ctggaagaca ctgcgcaggg cactggtgtc
     8161 gatgagctcc cgcagggcac gcacctcgct ctccgcgcgg cgggcatgga cctgcggacc
     8221 cagcacggca ttgccgactg tcgcgaagtc gaggagggcc ggcagcgagc gggccagtcg
     8281 ctggcccggc atctcggtcg tggtgccagc ggcgatcagg ccccacggcg cgccgtcgct
     8341 gctgagcggg acgtaggcac cgatgcgcag gccgacctcc agccaggcgc gcacgtaatc
     8401 gtccgcatcc ggcgcctcgc gccactcgtc gacccaggga ccgcgcaacg cgttggcctg
     8461 caggtaggcg gtccggtggg tgggcagcgg gcggccgacc gcgaccggcg ctcccggcgg
     8521 ggcgctgacg gcgagtgggg tggccgctgc accatcgaag gcgtagatga cgccgatgtc
     8581 gatgtcgcgg atgcgcagca gctcccgcag cagagcgtcc gccgtggcgt gggcgtcgct
     8641 cccggggcgg aaggagctca gggcggtcgc gatctccgcg cgctcggcga cctccgccgc
     8701 cagctcggcc tcgatctctc gctcgcgcgt gacgtcgaac atcacgccat ggatgaggat
     8761 gcggccgcgc gagtcgcgga cgggagtcgc ctcgtcccgg atccagaccg cgcctccatc
     8821 gcgggtgatg aggcggtact cggaggtgaa gcgctgctcg cctgcctcgc cggccgacag
     8881 ctcggcgacg gcccgctcgc ggtcgtcggg gtggagcgcg tcgacccagc ggtccgggtt
     8941 gagcagccac tcctccgcgc tgtagccgag cagcgactgg atctgcgggc tgatgtagat
     9001 gccgcgggct cccgggtcgt cgatgtacac gatcccgggc agcgtctcga ccaggagccg
     9061 gaagcgctcc tcggtctcac gcagcgccgc ctccgtcgcc cgctcgcgcg acagatcccg
     9121 gatgaaggcg cagaattcga cgcgcccctt gcgcgggagc gccgtgacgg ccagctcgac
     9181 cgggatctcg cgcccggagc gctgcagggc ctcgacctcc acccgacggt tgaggatccg
     9241 cgtctcaccg ctgcggaggt agcgcgccag gccggcgcgg tgcgcacgtc ggtatcgggc
     9301 cggcaggatg acctggtcca tgggcaggcc cagcacatcg ttggcccgcc agccgaagac
     9361 ctcctcggca cgcggattcc agccgacgat cgtcccctcg acgtccatgg tcactacggc
     9421 atccagggcg gaggccacaa cctgccccag ccgccccccg tccgtgtcgg ccaccacgcg
     9481 cctggtcctg gccgccatcc cgggcctgcc tacgccgcca cgacgcggtc gcggcccgcg
     9541 cgcttggcca tgaacatcgc catgtccgcc gtgcgcagca gctgctcgcg ggtggccgcc
     9601 gactcctcaa gctgggtgca gcccgccgag acggtcaccg tcagtcgcct cccatcttcg
     9661 tccacaaggt cgagcgaggc gagcgcgacc cgcacctgct cggcgatccg ctcggcatcc
     9721 tcgcgtccgg caccctccag gaccacgatg aactcctccc cgccgaggcg cgccaccagg
     9781 tcggccgcgc ggaaccggtc atccaggatt cgcgcgaacg agcggagcac gtggtcgcca
     9841 accaggtgcc cgtggtgctt gttgaagtcg ccgaaatggt cgagatcgaa gaggatggcc
     9901 gacaccgggc gccgcagggc gggctcggtg cggtgccagg aggcgatcat gcgatcggcc
     9961 gcctcgtcaa agtagcgtcg gttgtggagg ccggtcagcg ggtcgcggat ggcgagctgc
    10021 tccacctcgg cgtgcaggaa ggcgttcgcc agcgtgaggg ctgcatggcc cgccagcagc
    10081 tccatcgcct cgcgctcgat gggccggaag gcatcggtcc tgtcgacgcg aaggagggag
    10141 agggcgccga ccaccacgcc gtcgcgaacg agcgggatgg ccgcgcccag catgcgatcc
    10201 ggagcgggca cgtcgcggta gcgctccggg agagcggagc gctccaggtc cgcgtcgatc
    10261 accaggttgc gatcgcggat cgcccgtccg gccaggctct gccctggacg aatctcgact
    10321 cccagccagg ccgcatcacc gtcggtgatg gcgcgcacgt agtagcgacc cgtctgctgc
    10381 tccagcacgg tgagcaccac gacgtcggcg ggcaccaccc gacgtgcggc gtccaccatg
    10441 gccaccgcca gccggtcacg gtccagcgtg ccactgaccg cctgggtgaa ctcgtgcagg
    10501 ctgcggaaga cggccgc
//
LOCUS       DATACA010000203        15840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2195303, whole genome shotgun sequence.
ACCESSION   DATACA010000203 DATACA010000000
VERSION     DATACA010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 15840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15840
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195303"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VHK28_04190"
     CDS             complement(<1..979)
                     /locus_tag="VHK28_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2141463.1"
                     /translation="MATTTLTPRAEAYRRSWVIEWLTTVDHKKIGILYIINSFLFFFA
                     AGILALVIRSELMVPGMQFVDEATYNQAFTMHGTIMIFLFIVPVLAGFANYIVPLMLG
                     APDMAFPRINALSFWLLPLGGLLIFSGYLFGGAAAEGWTFYTPLSNARYAQGVGADLW
                     LIGLTLVGTASILGAINFIATIFKMRAPGMTLFRMPIFVWTVLVTATLIMLATPVLTA
                     GLIALFIDRNYGGAFFDPAAGGNPILWQHIFWFFGHPEVYIVILPAMGVVSEILPVFS
                     RKPLFGYRAFVFATVAIGLLSFSVWAHHMFTTGEVALPFFAFMTALIGVP"
     gene            complement(1001..2077)
                     /gene="coxB"
                     /locus_tag="VHK28_04195"
     CDS             complement(1001..2077)
                     /gene="coxB"
                     /locus_tag="VHK28_04195"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2141464.1"
                     /translation="MSRFLAAPWLRRAGRGGGPLLLIAALAGCGMIPPEPETETARDV
                     FTLFNIVLAMGVAVFIGVEAFIVYSIFRYRRRDDRLPQQLHGNTLVEIVWTAIPSVIV
                     LVLFVLSIITLTRVEARVEDPGVTIEVDGFQWQWAFRYLDDDDDPANDYTVIGTPGQP
                     PVMAVPVGEPVRLILRSEDVIHSFYVPHFLIKKDLVPLPEGEEPRDLEFTVTNEGTYS
                     GQCAEFCGDLHADMTFSVEAMSREAFDQWLAAGQAGETPAPSVDPEGEVVEVTADQIR
                     FDPLELTAPADQPFTLRFNNAEAVIHNVVIYDAENNEVFRSGDLTGPEATGDFAVPAL
                     PAGEYTYICEYHPVPDMTGTLIVE"
     gene            complement(2249..3142)
                     /locus_tag="VHK28_04200"
     CDS             complement(2249..3142)
                     /locus_tag="VHK28_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141465.1"
                     /translation="MRRTARILPAAMAASLLLTACGLGRPSDDGRIEHPTGDETVLRV
                     EHVGGFAPVEFLFTGMPVFTLLGDGRVITQGAQIDIFPGPALPAVQVRRLTEQGLQAV
                     LRAVADSGQFVGDAEWRGAQNFVADAGDTVFTLRAEGREVTITVHALGAFLPGEGQPG
                     VSGEELAAHEALGTLLERLTMLETTLPATAWAEPTARDYVPDGLRLLVRNADADPPDD
                     SGIASTEIAWPLEGDPADFGQPALDGYRCGAVTGADAGAWYEALAAANQLTRWTGGGH
                     RYQVTPRLLLPGEELACPEPA"
     gene            3220..4218
                     /locus_tag="VHK28_04205"
     CDS             3220..4218
                     /locus_tag="VHK28_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2141466.1"
                     /translation="MIHRLALAGAVLSALLATPKTLLAHGDDVPAPVLPDVLLAWSLD
                     PLPLLAIGLAGGAYLWAVRRVATTRRTPHPGWRTAAFLGGLVAMVLALSSPIEAYEGV
                     LFSVHMVQHMLLELVAAPLILVGAPATLALRAASPSVRRTLLSILHSRAIALLTFPLL
                     TWILFAAVNWGWHFSTLYDQALENVALHYFQHATFLGAALLFWFPVIGADPSRWRLPY
                     PARLFYLFLAMPQNSFLGVALMSASSVLYPHYLTNLRTWGPTALADQSLGGILMWVGG
                     DLVFLAAMGGVVAAWVRNEDRRTARLDARLDAEAAEAAEAEAAAQRSRSAGAAPGA"
     gene            complement(4215..6038)
                     /locus_tag="VHK28_04210"
     CDS             complement(4215..6038)
                     /locus_tag="VHK28_04210"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2141467.1"
                     /translation="MTRFAKLAVAAAIATYVLIAVGGLVRATDSGLACPDWPGCFAFN
                     DWIPAADAHVWIEHTHRLIAALAVGPMVGAIGLITVFTARRRDRVLLAAAVVAGVLVI
                     VQSILGREVVFQGLARELVSAHLAMALTVLAMIVVIADRAAHGPMPARRTGLPVGLIG
                     LTLALTFAQMLLGSWVTGHGAGLAYDDFPLMDGSLWPAIASHEQALHLAHRVLAVGVA
                     IAVVWMAVAVHRATDARRPRRLAAIAVALIGLQLVLGGANVWSRLSALFVVPHLAVGA
                     GLVAILVWLLLAALREPVAASTLAAGAGRTAADARSQSPALQAFRAYLALTKPRIIEL
                     LLVTTVPTMELAQRGIPSPWLVVAVMVGGSLAAGGANTINQFIDRDIDDVMRRTRHRP
                     LPSHAVTPARALAFGVVLSVISFVWLTLTVNLLSALLSLSAILFYVFVYSLWLKRSSP
                     QAVVIGGAAGCVPVLVAWAAVTGRIEVPALVLFAIVFYWTPPHFWALALRFRGDYAAA
                     KVPMMPVARGEAETARQIVLYSILLVAVSLLLLPAAQMGVIYLAAAVVLGVAFVWYAL
                     RVLHDVSDGRAAIRLFRFSSGYLTLLFVAVAVDSLVFVPFA"
     gene            complement(6132..6947)
                     /locus_tag="VHK28_04215"
     CDS             complement(6132..6947)
                     /locus_tag="VHK28_04215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX2141468.1"
                     /translation="MSSGDPGLFGPDSSVWRIDREVLILAGGTCALLMQLAHPSVAAG
                     VEQHSDFRGDPFGRLRRTLSATYAVAFGTRRRAQQAIDRMNAIHGVVRGRVPDSGQAY
                     AARDPELLLWVHATLIDTALRVYHRYVAPLSEGEQEAYHRESRLIATSLGVPEDRMPH
                     RLADLRAWMAAQLSDGPVRVTPVARRLAPAVLHPTRFPPRAVWNAGHLISFSVMPPAL
                     REGYGIAWGPGHESVMRGVAATTRRLLPLVPPVLRHVPAARAAERRAGSWSRT"
     gene            complement(6944..7675)
                     /locus_tag="VHK28_04220"
     CDS             complement(6944..7675)
                     /locus_tag="VHK28_04220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2141469.1"
                     /translation="MLPLGDQNEPWHGPALVTWALIAVNIGVFLLLQGGGSEGAFTYG
                     YSAIPLEITTGQDLLQTQTVTFNGQQFQIPQADGPEPIYLTLLTSMFMHGDWLHLGGN
                     MLFLWIFGDNIEHRAGRLLYLVAYLGAGLVGSLAQIVSTPDSPIPTLGASGAISGVLG
                     AYIVLFPRNRVTAIIFRFITQVPAIVAIGLWIAFQVVATLFSDPTIGGVAYWAHLGGF
                     GAGVVAGLLFRATPPRRPGRAARAW"
     gene            complement(7691..8713)
                     /locus_tag="VHK28_04225"
     CDS             complement(7691..8713)
                     /locus_tag="VHK28_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015292181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEX2141470.1"
                     /translation="MSTLPRRLATWKHVIDTANLPAGVAMDRVSKWLIVTRAAVFSMT
                     FTSGLIGGLLAIGADPGAVHYGYLALAVIGLVVAHAANNMINDYFDLEGGVDTDEYVR
                     ALYAPHPVLSGLLTKRQLGVAILVANAIDLAILLVLVAARGPLVAAFALAGLFISVFY
                     VAPPIRLKHRGLGEPGVFLVWGPLMIGGTFYAATGALPGWVWIASLPYAILVTTVLFG
                     KHIDKIGPDGARGVRTLPVILGEPLSKRIAQGLMIAFYPIVIGSALAGFIGPWVMLVI
                     LGLPRLVEVLGTFNRPRPDEPPPDYPVWPLWFVSAAFIHTRRAGALLILGLLLNVVIP
                     LRLPWT"
     gene            complement(8748..10340)
                     /locus_tag="VHK28_04230"
     CDS             complement(8748..10340)
                     /locus_tag="VHK28_04230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141471.1"
                     /translation="MQRRTWTLITVVLGSGIVFLDSTIVNVALRAIGEQLPATVIGRL
                     EGQSYIVNGYLLTLSALLILAGALADRYGRKRLFVIGLAGFGVTSLICGLSPTMEALV
                     VARLAQGSFGAMLVPTALALINAGFEGPARGRAFGVWAAATSALTLLGPPLGGLLVDT
                     FGWRIAFLINVPLVTVGVVVALTRIDESRNPNAPRSFDWLGSVAVAVAVGGLAYGAIR
                     GQQEAWTDPTAWFALALGAVATVALVPLMMYRRDPLMPPSLFASRNFSVTNVSTLLIY
                     GAIYVQGFLSAIFFQQTLGYTALASGLATLPMDVGLIFLSTTFGHLAGKYGPRRFMVA
                     GPLIMGIGLAWYLRIPADSARWAASPQDVATLLPPADYFVDVLPAALVFGLGLSIMVA
                     PLTTALMTSVPVGNSGLASAINNAISRIGPVLAGAVIFIAVSGAFYAGISARVPEVDT
                     TNVAVRDQLPPLNLAEEDDPAGLVQASREASTDAFRLAMLIAALLCLMGAAANLAISD
                     SVARGEEEPLPAGPAEESRPGS"
     gene            10401..11402
                     /locus_tag="VHK28_04235"
     CDS             10401..11402
                     /locus_tag="VHK28_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2141472.1"
                     /translation="MSTAALDRLLAWLVALQLATGLLSLRAGSPPTAVLFIVHGLLGG
                     ALLVAVAMKLWRSVPPAFEERRWGRLLLGFVLAAAALTALLGGFVWVASGRILSVGPW
                     TVLTLHVIAALVLVPIAVLHLLPRRWRLARPPAARPGKPRISRRTALATLGVAALGVA
                     AWTAAGVSDTLMGGLRRFTGSRWLPEGGVPPPTTFFGEGAEPIDPAAWRLTVKGAVGR
                     PLSLSLADLEALGREQRSEVLDCTSGWVLRTDWGGTPLSAVLDAARPMIDAREVTIRS
                     ITGWYARLPLPEARGALLATTVAGQPLPHGNGYPCRLVAPDRRGLEWVKWVTEVHIS"
     gene            11434..11682
                     /locus_tag="VHK28_04240"
     CDS             11434..11682
                     /locus_tag="VHK28_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX2141473.1"
                     /translation="MTVRTNLLLPKEPVDEVDRYAGPRGRSRYVAEALEARLRRDRLR
                     DAVRATAGKLMPEHYPQWRTSEQVVDWVRARRAEVTNP"
     gene            11709..12080
                     /locus_tag="VHK28_04245"
     CDS             11709..12080
                     /locus_tag="VHK28_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2141474.1"
                     /translation="MLDATFLIDHLRGDAGAVARWEGIFREGDQPFVNEVAVCEVRTG
                     LRPADEELLVRLLEPVEFVQPGPAAALTAGRWRWDASHKGKTLSLADALIAAAADALE
                     CAILTRNLRDFSLTPVPVETY"
     gene            complement(12081..12299)
                     /locus_tag="VHK28_04250"
     CDS             complement(12081..12299)
                     /locus_tag="VHK28_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141475.1"
                     /translation="MEERCRDCGRDVALGSPLYARRVTLIGETDPDVAYVCLDCRLRD
                     PLRDEAGNPISDEQLEGMKYMIGRGGRA"
     gene            complement(12305..13696)
                     /locus_tag="VHK28_04255"
     CDS             complement(12305..13696)
                     /locus_tag="VHK28_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEX2141476.1"
                     /translation="MDRWKTIIEPHRIKSVEPIRMTTVEERDRHLRDAGYNLFNLHAA
                     DVLIDLLTDSGTGAMSAEQWAGIQRGDESYAGSPSWYRFLTAVQELFPFKHVIPTHQG
                     RAAERILFGVVGGPGKVIPSNTHFDTTRANVEFTGAEAADLVIEEGRHPSVIHPFKGN
                     MDTDALDAFIRERGPDAIPLVMVTVTNNSGGGQPVSLENLRAVRAVCDRYGLPLFLDA
                     CRFAENAWFIKQREPGQAHRSIPEIVREMASLADGMTMSAKKDAIANIGGWLAMNDDA
                     WAEQARNLEILTEGFPTYGGLAGRDLEAIAQGLSEVVDEDYLRYRVRSTEYLGEGLIA
                     GGMPCVQPIGGHAVYIDAGALLPHIPPLEYPGQALAVALYREGGIRGCEIGTVMFGQH
                     PDGSETPAPMELVRLAIPRRAYTQSHVDYVIEVCRWVAERSGELRGYRIVEQPPQLRH
                     FTARFEPLAAVKA"
     gene            13763..14209
                     /locus_tag="VHK28_04260"
     CDS             13763..14209
                     /locus_tag="VHK28_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141477.1"
                     /translation="MRREEQEMGAWESLGGAPVSAPAVTAWAVDEMQVFCVFEDGELW
                     NRYWDGQAWHPWESLGGELVGNPAACSWGAERIDLFARGRDGRLWHRWYQPDGWQPWE
                     QLPVELTSDPAASSWGPNRLDVFGRGPDGDLLHAAWDGSAWSFDRG"
     gene            14219..>15840
                     /locus_tag="VHK28_04265"
     CDS             14219..>15840
                     /locus_tag="VHK28_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141478.1"
                     /translation="MTELGIRVRLILGALPRGLERLVRTAVGDGPAWEWLPHAVPILT
                     ALAAAVAIGALELAAFQTAADYPPLVAVGLGSLGAGIGGWGILLVPRAPTSLGARLVA
                     AAIGALAGGLLGTLLAEPIIRSPLLSAALAGLSGGLLVLGWRLQIAGTARAASRSIGL
                     VPAVIGALVVLAAFPLLQAGAELIITRATVFQFVERQVGFSSTLVALEGYAVLSAFEA
                     EPPIDPELGPDPRPHRWVLMRSDPGDRRMALVRTALLPGGLQDQAVTARVVDDPSATR
                     DAADDLRGRGVDVPGAVMERMLVVPPPASGLNPDDAVRLASLEELDEVADGTLVQVEL
                     RFLGEAVATCALAGECDARRLGAGSGTWEQLATGAVGERPVIVRTPYPPTLAPVVVYG
                     RQTADEGAVSRFTALPWIRDMTGWARLLRGAIVEHDPDLPVNRLWLGPILFGTTAVLL
                     WLGRRVRYPVFVPQAVAGVDGPAGGEVRALATGRIAPPGRSPVDLDGTPVTLRADDDG
                     ITITSVPLGALEVRVPRGLGALSGVEPGELRSL"
BASE COUNT         2387 a         5528 c         5687 g         2238 t
ORIGIN      
        1 tcggcacgcc gatcagggcc gtcatgaagg cgaagaacgg cagggccacc tcaccggtgg
       61 tgaacatgtg gtgggcccac acgctgaagc tcagcagccc gatggcgacc gtggcaaata
      121 cgaaggcgcg gtacccgaac agcggcttgc gactgaagac tggcaggatc tcgctgacca
      181 cgcccatggc cggcaggatg acgatgtaca cctcggggtg gccgaagaac cagaagatgt
      241 gctgccaaag gatggggttg ccgccggccg ccgggtcgaa gaaggcgccg ccgtaattgc
      301 ggtcgatgaa cagcgcgatc aggccggcgg tgagcaccgg cgtggccagc atgatcaggg
      361 tggcggtgac cagcaccgtc cacacgaaga tcggcatgcg gaacagcgtc atgcccggcg
      421 cacgcatctt gaagatggtg gcgatgaagt tgatcgcccc caggatcgag gcggtgccga
      481 ccagggtaag cccgatgagc cacaggtcgg cgcccacgcc ctgcgcgtag cgcgcgttgg
      541 agagcggcgt gtagaaggtc cagccctcgg ccgcggctcc gccgaagagg tagccggaga
      601 agatcagcag cccgcccagc ggcagcagcc agaaggacag ggcgttgatg cgcgggaagg
      661 ccatgtccgg tgcgccgagc atgagcggca cgatgtagtt cgcgaacccg gccaggaccg
      721 gcacgatgaa caggaagatc atgatcgtgc cgtgcatggt gaaggcctgg ttgtaggtgg
      781 cctcgtccac gaactgcatg ccgggcacca tcagctcgct gcggatgacc agggccagga
      841 tgcccgccgc gaagaagaac aggaagctgt tgatgatgta caggatcccg atcttcttgt
      901 ggtcgacggt cgtcagccac tcgatcaccc acgagcggcg gtaggcctcg gcgcgtggcg
      961 tcagggtggt ggttgccatg agtggttcgc tcgctctccg ctactcgacg atcagcgtgc
     1021 cggtcatgtc gggcacggga tggtactcac agatgtaggt gtactcgccg gccggcagcg
     1081 ccggcaccgc gaaatcaccg gtcgcctccg gcccggtcag gtctccgctg cggaacacct
     1141 cgttgttctc cgcgtcgtag atcaccacgt tgtggatcac cgcctcggcg ttgttgaagc
     1201 gcagggtgaa gggctggtcg gcaggggccg tcagctccag tggatcgaaa cggatctggt
     1261 cggcggtgac ctccaccacc tcgccctccg ggtcgaccga tggcgccggc gtctccccgg
     1321 cctgcccggc cgccagccac tggtcgaagg cctcgcggct catggcctcg acgctgaagg
     1381 tcatgtcggc gtgcaggtcg ccgcagaact cggcgcactg cccgctgtag gtcccctcgt
     1441 tggtcacggt gaactcgagg tcgcgcggct cctctccctc cgggagcgga accaggtctt
     1501 tcttgatcag gaagtgcggg acgtagaagg agtgaatcac gtcctccgac cgcaggatca
     1561 ggcgcaccgg ttcgccgacc ggcaccgcca tcaccggcgg ctgaccgggg gtgccgatca
     1621 ccgtgtagtc gttggccgga tcgtcgtcat cgtccaggta gcggaacgcc cactgccact
     1681 ggaaaccgtc cacctcgatg gtcacgccgg ggtcctccac gcgggcctcg acccgggtca
     1741 gggtgatgat cgagagcacg aacaggacca gcacgatgac cgatgggatg gcggtccaga
     1801 cgatctccac cagggtgttg ccgtgcagct gctgcggcag ccggtcgtca cggcggcggt
     1861 agcggaagat tgagtagacg atgaacgcct ccaccccgat gaagacggcc acgcccatgg
     1921 ccagcacgat gttgaacagg gtgaagacgt cgcgcgccgt ctccgtctcc ggctcgggcg
     1981 ggatcatccc gcagccggcc agcgcagcga tgagcaggag cggaccgcca ccgcggccag
     2041 cgcgccgcag ccatggcgcc gccaggaatc gtgacatcag ctcgtctcga ggctcctcac
     2101 ggtctcgtgg aacacaggga cctgccaggc cctgtgggac accaatgcag gccgcgcacg
     2161 tcctctccgg gctgacggcg gcacggccct attgtaccga tcggagcggg cgcgtcgcgc
     2221 gccctggttc actcgggttc cgacgtcctc aggccggctc cgggcaggcc agttcctcgc
     2281 ccggcagcag cagccgcggg gtgacctggt agcgatggcc gccgcccgtc cagcgcgtca
     2341 gctggttcgc cgccgccagc gcctcgtacc atgccccggc gtccgcgcca gtgaccgctc
     2401 cgcagcggta gccgtccagg gcgggctgcc cgaaatcggc cggatcccct tccagcggcc
     2461 aggcgatctc ggtgctggcg atgcccgagt cgtcaggcgg gtccgcgtcg gcgtttcgca
     2521 ccagcagccg caggccgtcg gggacgtaat cccgggcggt cggctccgcc caggcggtcg
     2581 ccggcagtgt cgtttcgagc atggtcagcc gctcgagcag ggtgcccagc gcctcgtgcg
     2641 cggccagctc ctccccggat acgcccggct gcccctcgcc gggaaggaag gcgccgagcg
     2701 cgtggaccgt gatcgtcact tcccggccct cggcgcgcag cgtgaacacg gtgtctcccg
     2761 cgtccgccac gaagttctgc gccccccgcc actccgcgtc tcccacgaac tggcccgagt
     2821 cggccacggc gcgcaggacg gcctgcagcc cctgctcggt gagccgccgg acctgcacgg
     2881 ccggcagcgc cggtcccggg aagatgtcga tctgggcgcc ctgggtgatg acccgcccgt
     2941 cgcccagcag agtgaagacc ggcatgccgg tgaacaggaa ctcgaccggg gcgaagccgc
     3001 ccacgtgctc cacgcgcagg accgtctcgt ccccggtagg gtgctcgatc cggccatcgt
     3061 cggagggacg ccccaggcca cacgcggtga gcagcaggga ggcggccatg gcggcaggga
     3121 ggatgcgggc ggttcgtctc atgcgccctc tgacgcctgg gccggccctt cggttccgcg
     3181 cgattcgccg attcctcgag gccgggccag aatgaggcgg tgatccaccg cctggccctg
     3241 gccggggccg tcctcagcgc cctgctggcc acacccaaga cgctcctggc gcacggcgac
     3301 gatgtgccgg caccggtgct gcccgacgtc ctgctggcct ggagcctcga cccgctaccg
     3361 ctgctggcca tcgggctggc cggcggcgcc tacctttggg cggtccgccg cgtggcgacc
     3421 acccggcgga cgccgcatcc gggatggcgc acggcggcct tcctgggcgg cctggtcgcc
     3481 atggtgctgg ccctgagctc gccgatcgag gcctacgagg gcgtcctgtt cagcgtccac
     3541 atggtgcagc acatgctgct ggagcttgtg gccgccccac tcatcctggt gggggccccg
     3601 gccacgctgg cgctgcgcgc ggcgtcgcca tcggtccggc gaaccctgct cagcatcctg
     3661 cacagtcgcg cgatcgccct gctgaccttc cccctgctga cctggatcct gttcgccgcc
     3721 gtcaactggg gctggcattt cagcacgctc tacgaccagg ccctggagaa cgtggcgctg
     3781 cactacttcc agcacgccac cttcctgggt gccgcgctgc tcttctggtt cccggtcatc
     3841 ggcgccgatc cgtcgcgctg gcgcctgccg tacccggcgc gcctcttcta cctgttcctg
     3901 gccatgcccc agaactcgtt cctgggggtg gcgctgatga gcgcctcgag cgtgctgtac
     3961 ccgcactacc tgaccaacct gcgaacctgg ggaccgaccg cgctggccga ccagtcgctg
     4021 ggcggcatcc tgatgtgggt cggcggcgat ctcgtgttcc tggccgccat gggaggcgtc
     4081 gtcgccgcct gggtccgcaa cgaggatcgc cggacggcca ggctggacgc gcggctggac
     4141 gccgaggccg cggaggcagc cgaggccgag gccgccgcgc aacgatcgcg ctcggcgggc
     4201 gccgcccccg gggcctaggc gaagggcacg aagaccaggc tgtcgacggc caccgccacg
     4261 aagagcaggg tcaggtagcc ggacgaaaag cggaagaggc ggatggcagc ccggccgtcg
     4321 ctcacatcgt gcaggacgcg cagcgcgtac cagacgaacg cgacgcccag caccaccgcg
     4381 gcggccaggt agatgacgcc catctgcgcc gccggcagca gcagcaggct gaccgccacc
     4441 agcaggatcg agtacaggac gatctggcga gcggtctcgg cctcgccgcg cgcgaccggc
     4501 atcatcggca ccttggccgc cgcgtagtct ccgcggaacc gcagggccag cgcccagaag
     4561 tggggcggcg tccagtagaa gacgatggcg aacagcacga gggccgggac ctcgatgcgg
     4621 ccggtcactg ccgcccaggc caccagcacc gggacgcagc cggccgcccc gccgatgacc
     4681 accgcctgcg ggctgctgcg cttgagccac aggctgtaga cgaagacgta gaacaggatc
     4741 gcgctcagcg acagcagcgc cgagagcagg ttcacggtca gtgtcagcca tacgaaggag
     4801 atcaccgaca gcaccacacc gaaggcgagc gcccgcgccg gcgtgaccgc gtggctgggc
     4861 agggggcgat ggcgggtgcg gcgcatgacg tcgtcgatat cgcggtcgat gaactggttg
     4921 atggtgttcg ccccgccggc cgccaggctg ccgccgacca tgaccgccac caccagccag
     4981 ggagagggga tgccgcgctg ggcaagctcc atggtgggga ccgtggtcac cagcagaagc
     5041 tcgatgatgc gcggcttggt gagcgccagg taggcgcgga atgcctgcag ggcgggactc
     5101 tgcgacctgg cgtcggctgc agtccgtccc gcgcctgccg cgagggtgct ggcagcgacc
     5161 ggctcgcgaa gggcggccag cagcagccag accaggatgg ccaccaaacc ggcgccgacc
     5221 gccaggtgcg ggaccacgaa cagggccgac agccgggacc acacgttggc cccacccagc
     5281 accagctgaa ggccgatgag cgccacggcg atggccgcca gccggcgcgg ccgccgcgcg
     5341 tctgtcgcgc ggtgcacggc gaccgccatc cacaccaccg cgatcgccac gccaaccgcc
     5401 aggacccggt gcgccaggtg gagggcctgc tcgtgcgatg cgatcgccgg ccacaggctg
     5461 ccgtccatca gcggaaagtc gtcataggcc agcccggcgc cgtggccggt cacccaggag
     5521 ccgagcagca tctgggcgaa cgtcaaggcc agcgtgagac cgatgaggcc caccggcagg
     5581 ccggtgcgcc gcgccggcat cggtccatgg gcggctcggt cggcgatgac cacgatcatg
     5641 gccaacacgg tgagcgccat ggccaggtgc gcgctgacca gctcgcgggc caggccctgg
     5701 aacaccacct cgcggcccag gatggactgc acgatcacca gcaccccggc gaccaccgcc
     5761 gccgcaagga gaacgcggtc gcggcgacgc gcggtgaaca cggtgatcag cccgatggct
     5821 ccgaccatgg gccccaccgc cagtgcggcg atcaaacggt gggtgtgctc gatccagacg
     5881 tgggcgtcgg cggccgggat ccagtcgttg aaggcgaagc agcccggcca gtcgggacag
     5941 gcaagtccag agtcggtggc gcgcaccagg ccaccgaccg cgatcaggac gtaggtcgcg
     6001 atcgcggcgg ccacggccag ctttgcgaac cgggtcaaca gggtcctccg gcgcgcctgg
     6061 cgcgggtcgc cgtcggcgcg agtgggtcga gtgggtgggc aatcgcatga tagccagccg
     6121 caccgcgctc gtcaggtgcg ggaccaagac cccgcgcggc gctcggcggc gcgggccgcg
     6181 gggacgtggc gcagcacggg tggtacgagg ggcagcagcc ggcgggtggt ggcggcgacc
     6241 cctcgcatca cggattcgtg gcccgggccc caggcgatgc catagccctc gcgcagggcc
     6301 ggtggcatga ccgagaagct gatcaggtgg ccggcgttcc agaccgcccg cggcgggaag
     6361 cgggtcgggt gcaggaccgc cggcgccagc cgccgggcca cgggcgtgac gcggaccggc
     6421 ccatcggaga gctgagcggc catccaggcc cgcaggtccg ccaggcggtg cggcatgcgg
     6481 tcctccggca cgccgaggct ggtggcgatc agccgggact cgcggtggta ggcctcctgc
     6541 tccccctcgg agagcggggc gacgtagcgg tggtacacgc gcagggccgt gtcgatcaag
     6601 gtggcatgca cccacagcag cagctccggg tcgcgcgccg cgtacgcctg gccgctgtcc
     6661 gggacgcggc cgcggaccac gccgtggatg gcgttcatgc ggtcgatggc ctgctgcgcc
     6721 cgccgccggg taccgaaggc gaccgcgtac gtggcggaca gcgttcgccg cagccggccg
     6781 aaggggtcgc cgcggaagtc agagtgctgt tcgacgccgg ccgccacgga tggatgcgca
     6841 agctgcatga gcagggcaca ggtgccgccg gccaggatca gcacctcgcg gtcgatgcgc
     6901 cacacgctgc tgtccggccc gaacaggccg gggtcgccgg agctcaccag gcgcgggcgg
     6961 cgcgtccggg gcggcgcgga ggtgtggcgc ggaagagcag cccggccacc acgccggcgc
     7021 cgaacccgcc aaggtgcgcc caataggcca cgccgccgat ggtcgggtcg ctgaacaggg
     7081 tggccaccac ctggaaggct atccacagcc cgatggccac gatggccggc acctgggtga
     7141 tgaagcggaa gatgatcgcg gtcacccggt ttctcgggaa gagcacgatg taggcgccca
     7201 gcacgccgga tatggcgccg gaggcgccca gcgtgggaat cggcgagtcg ggcgtgctca
     7261 cgatctgggc caggctgccg accagccccg cacccaggta ggcgaccagg tacagcagcc
     7321 gcccggctcg atgctcgatg ttgtcaccga agatccacag gaacagcatg ttgccgccca
     7381 ggtgcagcca gtcgccgtgc atgaacatgc tggtcagcag ggtcaggtag atcggctccg
     7441 gcccatccgc ctgcggaatc tggaactgct gcccattgaa ggtgaccgtc tgcgtctgga
     7501 gcagatcctg gcccgtggtg atctccagcg ggatggcgct gtatccgtag gtgaaggcac
     7561 cttcgctgcc gccgccctgc aacagcagga agacgccgat attgaccgca atcagcgccc
     7621 aggtgaccag cgccggcccg tgccagggct cgttctggtc gcccagcggc agcatcggtc
     7681 ctgcgtccgc ctaggtccag ggcagccgaa gcgggatgac cacgttcagc agcagcccca
     7741 ggatcagcag cgcacccgcc cgacgggtgt ggatgaaggc agcgctcacg aaccacaggg
     7801 gccagacggg atagtccggc ggcggctcgt ccggtcgcgg tcgattgaag gtgccgagca
     7861 cctcgaccag gcgcgggaga cccaggatga cgagcatgac ccacggaccg atgaagcccg
     7921 ccagcgccga accgatgacg atcgggtaga aggcgatcat cagcccctgc gcgatgcgct
     7981 tggagagcgg ctcgcccagg atcaccggca gggtgcgcac gcctcgcgcc ccatccggcc
     8041 cgatcttgtc gatgtgcttg ccgaacagga cggtggtcac caggatcgcg tacggcagcg
     8101 aggcgatcca gacccacccg ggcagggcgc cggtcgcggc gtagaacgtg ccaccgatca
     8161 tgagcggtcc ccagaccagg aagacgcccg gctcacccag cccgcggtgc ttgaggcgga
     8221 tgggcggcgc cacgtagaag acgctgatga agagccccgc cagggcgaag gccgcgacca
     8281 gcgggccgcg cgccgcgacc agcaccaaga ggatggccag gtcgatggca ttggccacca
     8341 ggatcgccac ccccagctgc cgcttggtga gcaggccgga gagcacaggg tgcggcgcgt
     8401 agagggcccg aacgtactcg tcggtgtcga cgccgccctc caggtcgaag tagtcattga
     8461 tcatgttgtt ggcggcgtgc gccaccacca ggccgatgac cgccagcgcc aggtacccgt
     8521 agtggacggc ccctggatcg gcaccgatgg ccagcaggcc gccgatgagt ccggaggtga
     8581 aggtcatgct gaagaccgcc gcccgggtca cgatcagcca cttgctgacg cggtccatgg
     8641 cgacgcccgc cggcaggttg gcggtgtcaa tgacgtgctt ccaggtggcg aggcgacgcg
     8701 ggagcgtgga catacgcgga tcatagccgc gcccgcagcg catgccgtca gctgcccggt
     8761 ctggactcct cggcgggtcc agctggcagc ggctcctcct cgccgcgcgc caccgagtcg
     8821 ctgatcgcca ggttcgcggc ggctcccatt aggcagagga gggcggcgat cagcatcgcc
     8881 agccggaagg cgtcggtcga cgcttcgcgt gaggcctgca ccagcccggc tggatcgtcc
     8941 tcctcggcca ggttgagcgg cgggagctgg tcacggaccg cgacgttggt ggtgtctacc
     9001 tccggcacgc gggcgctgat gcccgcgtag aaggcgccgg acaccgcgat gaagatcacc
     9061 gccccggcca gcaccggacc gatgcggctg atggcgttgt tgatggcgct tgccagtcca
     9121 gagttgccca ccggcaccga cgtcatcaga gcggtggtca gcggggcgac catgatcgac
     9181 agccccaggc cgaagaccag ggcggccggc agcacgtcca cgaagtagtc ggctggcggc
     9241 agtagcgtgg cgacgtcctg cgggctggcc gcccatcggg cgctgtcggc cgggatgcgc
     9301 aggtaccagg ccaggccgat gcccatgatc agcggcccgg ccaccatgaa ccgtcgcggc
     9361 ccgtacttgc ccgccaggtg cccgaaggtg gtcgacagga agatgaggcc cacgtccatg
     9421 ggcagggtgg cgaggcccga ggccagtgcc gtatagccca gcgtctgctg gaagaagatc
     9481 gcggacagga acccctgcac gtagatggcg ccgtagatga gcagcgtcga cacgttggtg
     9541 acgctgaagt tccgcgacgc gaagagcgat ggcggcatca gcggatcgcg ccggtacatc
     9601 atcagcggca ccagcgccac ggtggcgacg gcgccgaggg cgagagcgaa ccacgcggtg
     9661 ggatcggtcc aggcctcctg ctggccgcgg atggcaccgt acgccagccc tcccacggcc
     9721 acggccaccg ccaccgagcc cagccagtcg aagctgcgcg gcgcgttcgg gttgcggctc
     9781 tcgtcgattc gagtcagcgc caccaccacc cccaccgtca ccagtggcac gttgatcagg
     9841 aacgcgatcc gccagccgaa agtgtcgacc aggaggccgc cgagcggcgg gcccagcagt
     9901 gtcagggcgc tggtcgccgc cgcccagacg ccgaacgcac ggccgcgcgc cggcccctcg
     9961 aagccggcgt tgatcagcgc cagggcggtc ggcaccagca tcgccccgaa ggagccctgc
    10021 gccagccgag cgacgaccag ggcctccatg gtcggcgaca ggccgcagat gagcgaggtc
    10081 acgccgaacc cggccaggcc gatgacgaac agccgcttgc gcccgtaccg atcggccagg
    10141 gctccggcca ggatcaggag cgcggagagc gtcagcaggt agccgttgac gatgtaggac
    10201 tggccctcca gccgcccgat gactgtggct ggcagctgct ccccgatggc gcgcagtgcg
    10261 acgttgacga tggtggaatc gaggaacacg atccccgacc ccagcacgac ggtgatgaga
    10321 gtccaggttc ggcgctgcac ccgcggagcc tagcaaagcc gcctcgccgg ctcggccgcc
    10381 gcggagcgta ggatggaccg atgagcaccg cggccctcga ccgcctgctc gcctggctgg
    10441 tcgccctcca gctggccacc gggctcctca gcctgcgcgc cggatcgccg cccaccgccg
    10501 tgctgttcat cgtgcacggc ctgctgggtg gtgccctgct ggtggcggtc gccatgaagc
    10561 tgtggcgatc ggtgccgccc gcgttcgagg agcgacgctg gggccgcctg ctgctcggct
    10621 tcgtgctggc ggctgccgcc ctgaccgccc tgctgggtgg cttcgtctgg gtcgcgtcgg
    10681 gtcggatcct gtccgtcggc ccctggacgg tattgaccct tcacgtcatc gcggcgctgg
    10741 tgctggtgcc gatcgccgtc ctgcacctgc ttccccgtcg ctggcgcctg gcgcgacccc
    10801 cggccgcgcg gcccggcaaa ccgcgcatct cgcgccgcac ggccctggcc acgctgggag
    10861 tggcggcgct gggagtggcc gcctggacgg ccgcgggcgt gtccgacacg ctgatgggcg
    10921 gcctgcggcg cttcaccggc tcgcgctggc tgcccgaggg cggggtgccg ccgccgacca
    10981 ccttcttcgg agagggcgcc gagccgatcg atccggcggc atggcggctg accgtgaagg
    11041 gcgcggtggg acggccccta tccctgtcgc tggcggacct cgaagcgctg gggcgcgagc
    11101 agcgctcgga ggtgctggac tgtacctccg gttgggtgct gcgcacggac tggggcggga
    11161 cgccgctgtc cgccgtgctg gacgcggccc ggcccatgat cgacgcgcgt gaggtcacga
    11221 tccgatccat caccggctgg tacgcccgcc tgccgctgcc cgaggcgcgc ggtgccctgc
    11281 tggccacgac ggtcgctggc cagccgctcc cccacggcaa cggctatccc tgccggctgg
    11341 tagcccccga tcgccggggg ctggagtggg tgaagtgggt caccgaggtc cacatctctt
    11401 gacatgcacg gtagattgcg catactcgtg cgcatgactg tgcgcacgaa cctgctgctc
    11461 cccaaggaac cggtcgacga ggtggaccga tacgccggac ctcgcgggcg cagccgctac
    11521 gtggcggagg cgctggaggc caggctccgc cgtgaccggc tgcgcgacgc ggttcgtgcc
    11581 accgccggga aactcatgcc cgagcactat ccgcagtgga ggacgtcgga gcaggtcgtg
    11641 gactgggttc gtgctcgccg ggcagaagtc acgaatccct gaggactctt ttccagacca
    11701 tgcgcttgat gctggacgcc accttcctca tcgaccacct acgaggtgac gcgggcgcgg
    11761 tcgcgcgctg ggaaggcatc ttccgggaag gcgaccagcc cttcgtcaac gaggtcgcgg
    11821 tgtgcgaggt ccggacggga ctccgtcctg cggacgagga gctgctcgtg cgactgctcg
    11881 agccggtcga gttcgtccaa cccggtccag ccgccgctct cacggcgggc cgctggcgct
    11941 gggatgcctc gcacaaggga aagacgctca gtctcgctga cgcgctcatc gcggccgctg
    12001 ccgacgcact ggagtgcgca atcctgacgc gtaacctacg cgacttctcc ctgacgcctg
    12061 tgccggtgga gacgtattag ctaggccctg ccgccgcggc cgatcatgta cttcatcccc
    12121 tccagctgct catcgctgat cgggttgccc gcctcgtcgc ggagcggatc gcgcagccgg
    12181 cagtccagac acacgtacgc cacgtccgga tccgtctcgc cgatgagggt gacccgccgc
    12241 gcgtagagcg gcgaacccag ggccacgtcc cgcccgcagt cccggcaccg ttcctccatc
    12301 gggctcaggc cttcaccgcg gccagcggct cgaagcgcgc cgtgaagtgc cgcagctgcg
    12361 gcggctgctc cacgatccgg tagccgcgca gctccccgga gcgctcggcc acccagcggc
    12421 aaacctcgat cacgtagtcg acgtgcgact gggtgtaggc gcgacgcggg atcgccagcc
    12481 gcaccagctc catgggtgca ggcgtctcgg agccgtccgg gtgctgcccg aacatcacgg
    12541 tgccgatctc gcagccgcgg atgccgccct cgcggtagag cgccacggcc agcgcctgcc
    12601 cggggtactc cagcggaggg atgtgcggca gcagcgcgcc ggcgtcgatg tacaccgcgt
    12661 gcccgccaat gggctgcacg cagggcatgc cacccgcgat cagcccctcg ccgaggtact
    12721 cggtggagcg cacccggtag cgcaggtaat cctcgtcgac cacctcgctc aggccctggg
    12781 cgatggcctc caggtcgcgg cccgccaggc cgccataggt cgggaagccc tcagtcaaaa
    12841 tctccaggtt gcgcgcctgc tccgcccagg cgtcgtcgtt catggccagc cagccgccga
    12901 tgttggcgat ggcgtccttc ttggcgctca tggtcatgcc atccgccagc gaggccatct
    12961 cgcggacgat ctccgggatg gaccgatgcg cctgccccgg ctcgcgctgc ttgatgaacc
    13021 aggcgttctc cgcgaagcgg cacgcgtcca ggaatagcgg cagtccgtat cggtcgcaga
    13081 cggcgcgcac cgcgcgcagg ttctccaggc tgaccggctg accgccgccg gagttgttgg
    13141 tgaccgtgac catgaccagc gggatggcgt ccggcccgcg ctcgcggatg aacgcatcca
    13201 gcgcatcggt gtccatgttg cccttgaacg gatggatgac cgatgggtgc ctgccctctt
    13261 cgatcaccag gtcggccgcc tcggcgcccg tgaactcgac gttggcccgg gtggtgtcga
    13321 aatgggtgtt cgacgggatc accttgcccg gcccgcccac cacgccgaac aggatccgct
    13381 cggccgccct gccctggtgg gtgggtatga cgtgcttgaa cggaaagagc tcctggacgg
    13441 cggtcaggaa tcggtaccac gacggcgagc cggcgtagct ctcgtccccg cgctggatgc
    13501 cggcccactg ctcggccgac atggcgccgg tgccggagtc ggtcagcagg tcgatcagga
    13561 cgtcggcggc atgcaaattg aagaggttgt agcccgcgtc gcgcaggtgt cggtcacgct
    13621 cctcgaccgt cgtcatccgg atcggctcga cgctcttgat ccggtgcggc tcgatgatcg
    13681 tcttccatcg gtccatccgc atcagagagt agcggttcgc ggcgccgggc tccctagaat
    13741 gtgtcggcga gcgagaggca ggttgcggcg agaggagcag gagatgggcg cgtgggagtc
    13801 gctgggtgga gcgccggtgt cggcgccggc ggtgacggcc tgggccgtgg acgagatgca
    13861 ggtcttctgc gtgttcgagg acggcgagct ctggaaccga tactgggacg ggcaggcgtg
    13921 gcacccctgg gagtcgctgg gcggcgagct ggtgggcaac ccggccgcct gctcctgggg
    13981 agcggaacgc atcgacctct tcgcccgcgg gcgggatggc cgcctctggc accgctggta
    14041 ccagcctgac ggctggcagc cgtgggagca gctgcccgtc gagctgacct cggacccggc
    14101 ggccagctcc tggggtccca accgactgga cgtcttcggc cgcggccctg acggcgacct
    14161 gctgcatgcc gcctgggacg gcagcgcctg gtccttcgac cgcggctgag gccgccgcgt
    14221 gaccgagctc ggcatccgcg tccgactgat cctgggcgcc ctgccgcgcg gcctcgagcg
    14281 gctggtgcgc acggcggtcg gggacggccc cgcctgggaa tggctgccgc acgccgtccc
    14341 catcctgacg gcactggcgg cagccgtggc tatcggtgcc ctggagctgg ccgcgttcca
    14401 gaccgcggcc gattacccgc ccctggtggc cgtgggcctg ggttcgctgg gtgccggcat
    14461 cggtggctgg ggcatcctgc tcgtgcctcg tgccccgacc tccctagggg ctcgcctggt
    14521 cgccgccgcg atcggcgcgc tcgccggtgg cctgctgggg acgctgctgg cggagcccat
    14581 cattcgctcg ccgctgctgt cggccgccct ggccgggctg tccggcgggc tgctggtgct
    14641 cggctggcgg ctgcagatcg ccggcaccgc tcgggccgcc tcgcggtcca tcggcctggt
    14701 cccggccgtg atcggcgccc tggtcgtgct ggcggcgttc ccgctgctgc aggccggcgc
    14761 cgagctgatc atcacccgcg ccacggtctt ccagttcgtg gagcggcagg tgggcttcag
    14821 ctcgacgctg gtggccctcg agggctacgc cgtgctgtcc gcgttcgagg ccgagccgcc
    14881 catcgacccg gagctggggc ctgacccgcg cccccaccgc tgggtgctga tgcgcagcga
    14941 ccccggcgat cggcgcatgg ccctggtgcg cactgcgctg ctgcccggtg ggctccagga
    15001 tcaggcagtc accgcccgcg tcgtcgacga tccatccgcg acacgtgatg cggcggacga
    15061 cctgcgcggc cgcggtgtgg atgtccccgg ggcggtcatg gaacggatgc tggtggtgcc
    15121 gcccccggcc tcgggcttga acccggacga cgcggtgcgg ctggcatcgc tggaggagct
    15181 ggacgaggtc gccgacggca ccctcgtgca ggtggagctt cggttcctgg gggaggcggt
    15241 ggcgacctgc gcgctcgccg gggaatgcga tgcgcgtcgg ctcggcgctg gcagcggcac
    15301 ctgggaacag ctcgccacgg gggcagtggg cgagcgcccg gtgatcgtgc gcactccgta
    15361 ccctccgacg ctggcgccgg tcgtggtcta tggccggcag actgccgacg agggcgcggt
    15421 cagccgcttc accgcgctgc cctggattcg ggacatgacc ggctgggcgc ggttgctgcg
    15481 cggcgccatc gtcgagcacg acccggacct gcccgtcaac cgcctgtggc tgggacccat
    15541 tctcttcggc acgacggcgg tgctgctgtg gctgggacgc cgcgtccgct atccggtctt
    15601 cgtgccgcag gcggtcgcgg gcgtggacgg cccagccggc ggcgaggtgc gtgcactggc
    15661 caccggccgc atcgcgccac ccggccgctc cccggtggat ctcgacggca caccggtcac
    15721 gttgcgtgcc gacgatgacg ggatcacgat cacctcggtt cccctagggg cgctcgaggt
    15781 ccgcgttccg cgcggcctcg gggcgctatc gggcgtcgaa cccggcgagc tccgctccct
//
LOCUS       DATACA010000204         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2195316, whole genome shotgun sequence.
ACCESSION   DATACA010000204 DATACA010000000
VERSION     DATACA010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195316"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..303)
                     /locus_tag="VHK28_04270"
     CDS             complement(46..303)
                     /locus_tag="VHK28_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141479.1"
                     /translation="MEFERLRLQVRLVRAVWRSERARPEPNVNLQELQKRSLVVLDDV
                     AFHIGNGQDRELAELIASVRAEVQTGGSVAPGDDKERGRES"
     gene            398..473
                     /locus_tag="VHK28_04275"
     tRNA            398..473
                     /locus_tag="VHK28_04275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:431..433,aa:Arg,seq:ccg)
     gene            complement(487..948)
                     /locus_tag="VHK28_04280"
     CDS             complement(487..948)
                     /locus_tag="VHK28_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141480.1"
                     /translation="MDERDRFPAPAADGDGLGGLTGATGNLTPDDPDDEFVPAETREI
                     GDPRTARDLTAASYERGEKRRAESSDAPGRLVDEGERTAPNDRDGGYGSEHGLAGSDP
                     AYRFEERAGGASGAMPDVGREHGASEPAHRRESRPPRLGVDEVHSPEDEHL"
     gene            complement(964..1428)
                     /locus_tag="VHK28_04285"
     CDS             complement(964..1428)
                     /locus_tag="VHK28_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2141481.1"
                     /translation="MSTVEKSIDVNVPLRTAYNQWTQFEEFPRFMEGVKSVRQLDDTH
                     LHWIAEVGGQDKEWDAEITEQEPDQRIAWTSTTGARNAGVVTFHRIDDDTTRVTLQMD
                     VEPEGVMESAGDALGVLDGRVEGDMERFKEFIEERGAPTGAWRGEVQGGGTR"
     gene            1574..1735
                     /locus_tag="VHK28_04290"
     CDS             1574..1735
                     /locus_tag="VHK28_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141482.1"
                     /translation="MPEGWSDKRERQYEHIKESYKERGVKNDEAEERAARTVNKERSE
                     EGETKKDRD"
     gene            1783..>2628
                     /locus_tag="VHK28_04295"
     CDS             1783..>2628
                     /locus_tag="VHK28_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2141483.1"
                     /translation="MKADSAARLLRRDRLAREAAEKELSPAATRSTLSLGRQRDEEPD
                     PFRTAFERDRDRIIHAKAFRRLKHKTQVFLHPDGDHYITRLTHTLQVAQIARALASAL
                     TLNEPLAEAIALGHDVGHTPFGHTGEEALSPYFPPDGWHHAAQSVRILEVLEDLNLSW
                     EVRDGIRAHSWRIDPPPATAEAMCVRYADRIAYLTHDALDAMRAGVLSRDAFPQEALR
                     VFGEPGRGWIGEMIEAVIDHSLESGEVRMDAGTLSVMNELRDFMFERVYQSDEQRGQQ
                     LNAIAV"
BASE COUNT          390 a          918 c          894 g          426 t
ORIGIN      
        1 cccggtggct gagcaccggc tcgactatcc cccggcgagg gcgcctcagg actcccggcc
       61 acgctccttg tcgtctccgg gggccacgga gcccccagtc tgcacctcgg ctcgcaccga
      121 ggcgattagc tcggcgagct cccggtcctg cccgtttccg atgtggaagg caacatcgtc
      181 caggacgacc agggagcgct tctgcagctc ctgcaggttc acgttcggct cgggtcgtgc
      241 ccgttcggag cgccagacgg cccgtaccag ccgaacttgc aaccgcagcc gttcgaattc
      301 catcttggac tggagttcag caagtgggat gccctttggg gcttctggcc cttcgactgc
      361 gcctgggtta cgatggcgcc tcggcgaatt tgacctggcg ccggtagctc agtggataga
      421 gcgtcggcct ccggagccga aggtcgcagg ttcgaatcct gtccggcgcg ccagacccag
      481 aacgccctaa aggtgctcgt cctccggaga gtgcacctcg tccacgccga gacgcggtgg
      541 ccgcgattcg cggcgatggg ccggttcgct ggcgccatgc tcgcgtccta catctggcat
      601 cgcccctgag gcgccgccgg cccgctcctc gaaccggtag gcggggtcgg acccggccag
      661 gccgtgctcc gacccgtagc cgccatcccg gtcgttcggc gcggtccgct ccccctcgtc
      721 caccagccgg cccggagcgt ccgagctctc ggctcgacgc ttctcgcccc gctcgtatga
      781 cgcagccgtc aggtcgcgcg cggtgcgagg atcgccgatc tcccgcgtct cggcgggcac
      841 gaactcgtcg tccggatcgt ccggggtcag gttgccggtc gcgccggtca ggccacccag
      901 gccatcaccg tccgcggccg gcgccgggaa gcgatcgcgc tcgtccatgg ttcgtgacca
      961 gggtcagcgg gtgccgccgc cctggacctc tccgcgccac gcaccggtcg gcgcgccgcg
     1021 ctcctcgatg aactccttga accgctccat gtcgccctcg acacgtccgt cgagcacgcc
     1081 cagggcgtcg ccggcagact ccatgacccc ctccggctcg acgtccatct ggagcgtcac
     1141 gcgcgtggtg tcgtcgtcga tgcggtggaa ggtgaccacg ccggcattgc gcgcgccggt
     1201 ggtgctggtc caggcgattc gctggtcggg ctcctgctcg gtgatctcgg catcccactc
     1261 cttgtcctgg ccgccgacct cggcgatcca gtggaggtgg gtgtcgtcga gctgtcgcac
     1321 cgacttcacg ccctccatga agcgcgggaa ttcctcgaac tgcgtccact ggttgtacgc
     1381 ggtccgcagc gggacattga cgtcgatgga cttctcaacg gtgctcattc ggttcctcct
     1441 cggggattgg gttgcgtttc gaggttcatg gggtgcaagc ggcgggccag caggacgcgt
     1501 ttttggcgct ggcacgttcg gtgcaacccg caaatcggtc gtcgccggtc cgggccgtca
     1561 ggaggatgga ccgatgcctg aaggttggag cgacaagcga gagcgccagt acgagcacat
     1621 caaggagtcg tacaaggagc gcggcgtcaa gaacgatgag gccgaggagc gcgccgcgcg
     1681 caccgtgaac aaggagcgga gtgaggaggg cgagaccaag aaggacaggg actagcgcgg
     1741 atctggccca tcaaggccgg gaggatcggc catcatcggt ccatgaaggc cgattccgcc
     1801 gcgcgcctgc tgcgccgcga ccgcctggct cgcgaggccg ccgagaagga gctcagcccg
     1861 gccgctaccc gatccacgct cagccttggg cggcagcgcg acgaggagcc ggatcccttt
     1921 cggaccgcct tcgagcgtga ccgggaccgg atcatccatg ccaaggcctt tcgccggctg
     1981 aagcacaaga cccaggtctt cctgcacccc gatggcgacc actacatcac gcggctgacg
     2041 cataccctgc aggtagcgca gattgcgcgg gcgctggcgt cggcgctgac cctcaacgag
     2101 ccgctggccg aggccatcgc cctcggccac gacgtgggac acacgccgtt cggccacacc
     2161 ggtgaggagg cgctgtcccc gtacttcccg cccgacggct ggcaccatgc ggcccagagc
     2221 gtccgcatcc tggaggtgct ggaggatctg aacctgagct gggaggtccg cgacgggatc
     2281 cgggcccact cgtggcgcat cgacccgccg ccggccacgg ccgaggcgat gtgcgttcgc
     2341 tacgcggacc gcatcgcgta cctgacccac gacgcgctgg atgccatgcg cgcgggcgtc
     2401 ctgtcgcgcg acgccttccc gcaggaggcg ctgcgcgtct tcggtgagcc gggccgcggc
     2461 tggatcggcg agatgatcga ggcggtgatc gaccactccc tggagtcggg tgaggtacgc
     2521 atggacgcgg gcacgctgtc ggtcatgaac gagctgcgcg acttcatgtt cgagcgcgtc
     2581 taccagtcgg acgagcagcg cggccagcag ctgaacgcca tcgcggta
//
LOCUS       DATACA010000205         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1553412, whole genome shotgun sequence.
ACCESSION   DATACA010000205 DATACA010000000
VERSION     DATACA010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553412"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="VHK28_04300"
     CDS             complement(<1..1144)
                     /locus_tag="VHK28_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141484.1"
                     /translation="MERPTVLLALSENADEYTAALTRAGFDVTDDTAGVGLRIAQGDA
                     PELAVLDCDLPEAEAQQLYDTLHGDPAVPTLLLFGAEIPEFATSSTNVTFDEYGIKPT
                     PAEALVYRLHALLIRGGRTMPGEGGTWTPSTSEDGTAIGEGHIVSVFAPKGGVGKTTV
                     AVNVAVALREQTRSRVLLFDADVGVGNVTSVLAAPYRLGLSDLSDSDPSEWTDAAFEQ
                     ATSTHASSGIRVLTWGQDPGESERVTVDLMLAALRWARAHHAYVVVDTHPGYDDRTMA
                     MLTVANEILLVVTPEVGAIRNSSQFLDLAREVGLRDVVRVVVNRANHGVRLPDIEQTL
                     GLKVSATVVSNGPKAVIAANEGLPVVTKYPKDRIATDLHDVARLVTA"
     gene            complement(1273..2001)
                     /locus_tag="VHK28_04305"
     CDS             complement(1273..2001)
                     /locus_tag="VHK28_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141485.1"
                     /translation="MSFLRRPRKQESPPPPPTPMQEEVEAQQYAVKLNFLARSSDGLR
                     MQPDPAVRAALPGVVEPLASTSVEVLEPLPLEYADAAPTMERFNEMQQWVLARRDVSP
                     IGRHGLYVLEMTDALDMTVDTFYCALLHGETDTSGYREYNAIVGGLASHWDELSGELI
                     VRAVLGWGGKGLRGDTDRIGQRILSSLYQQVIASGYSLGAAESARAAVHGRTGVVCAH
                     CGFEAAGAGAFYCPKCGMRMTRSG"
     gene            complement(2097..>2990)
                     /locus_tag="VHK28_04310"
     CDS             complement(2097..>2990)
                     /locus_tag="VHK28_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2141486.1"
                     /translation="AARFVLGARPEPLAPVEGEATLPERWIHSRLSATIERATRQLDE
                     LDLAGYAATVYDFAWSDYCDWFLETAKIDLRREEASLGERARTWRTLADVLAATLRLL
                     HPILPFVTEEIWATLREVNAEATAGERLLISAAWPAPAARDVGAEDAMQRVIAATREV
                     RDLRTQQQLPAGEWRSVRVASGSAQAEDILRQAEPYLGQLWRARPLEILLAAATHDFP
                     EHAAATALGYAWIEAGGAEVQRAAQRAQKQRAELEEQIRRVRGLLASEGFLAKAPPQV
                     VQRERERLAELEERLAGLDVQ"
BASE COUNT          449 a         1068 c         1022 g          451 t
ORIGIN      
        1 cagcggtcac gaggcgagcg acgtcgtgca ggtcggtcgc gatgcggtcc ttcggatact
       61 tggtcaccac cggaaggccc tcgttggcgg cgatgactgc cttcggacca ttcgacacga
      121 cggtcgcact cactttcaaa cccagggtct gctcgatgtc cggcaggcgc acgccgtgat
      181 tggcgcggtt gaccaccacg cgaaccacgt cgcgcaggcc cacctcgcgc gcgaggtcca
      241 ggaattggct gctgttgcga atggctccga cctcgggggt gaccaccagc aggatctcgt
      301 tggcgaccgt cagcatggcc atggtgcggt cgtcgtagcc cggatgggta tcgaccacga
      361 cgtaggcgtg gtgggcacgc gcccagcgca gcgcggccag catcaaatcg acggtgaccc
      421 gctcggactc gcccgggtcc tgtccccagg tcagcacccg gataccgctg gaggcgtggg
      481 tactggtggc ctgctcgaag gcggcatccg tccactcaga cggatcggag tcggagaggt
      541 ccgacagacc cagccgatac ggagcggcca gcaccgaggt gacattgccc acgccgacgt
      601 cggcgtcgaa gagcaacact cgcgagcggg tctgctcgcg cagggctacc gcgacgttga
      661 cggccaccgt cgtcttgcca acgccgccct tgggggcgaa gacgctgacg atatggccct
      721 cgccgatggc ggttccgtcc tcgctggtgg atggcgtcca ggtgccgccc tcgccgggca
      781 tcgtgcgacc gccacggatc aggagggcgt gcagccggta gaccagcgcc tcggcgggcg
      841 tcggcttgat gccgtattcg tcgaaggtga cattggtgct cgacgtcgcg aactccggga
      901 tctcggcgcc gaacagcagc agggtcggga cggccggatc gccgtgcagg gtgtcgtaca
      961 gctgctgggc ctcggcctcg ggaaggtcgc agtccaggac ggccaactca ggggcgtcgc
     1021 cctgggcaat gcgcagcccg acaccggccg tgtcatcggt tacgtcgaag cccgctcggg
     1081 tcagcgccgc ggtgtactca tccgcgttct cggacagcgc gagcaggaca gtaggtcgct
     1141 ccatgggtcc ccttcgatag tgcttcggga cagtgacgga ccgcacaact atccgccgca
     1201 cccatccgca cgacaatggg actcaggcct gcccggatgc cccgaaggta ctgcccccca
     1261 cggaccgatg cctcagccgg acctcgtcat tcgcatgccg cacttcggac agtagaaggc
     1321 accagcgccg gcggcctcga agccgcaatg ggcgcacacc acgccggtac ggccatgcac
     1381 ggctgcccgc gccgactccg cggcgcccag gctgtagccg gacgcgatca cctgctggta
     1441 cagcgagctc aagatgcgtt gaccgatgcg gtccgtgtcg ccgcgcaggc ccttgccgcc
     1501 ccagccgagc acggcccgca cgatcagctc gccggacagc tcgtcccagt ggctggccag
     1561 cccacccacg atggcgttgt actcgcggta gccgctggta tcggtctcgc cgtggagcag
     1621 cgcgcagtaa aaggtgtcaa ccgtcatgtc caaggcatcg gtcatctcga ggacgtagag
     1681 gccgtgccgc ccgatggggc tgacgtcgcg tcgggcgagc acccactgct gcatctcgtt
     1741 gaagcgctcc atggtgggtg ccgcgtccgc gtactccagg ggcagcggct cgaggacctc
     1801 cacggatgtg ctggccagcg gctcgacgac gcccggcaac gccgcgcgga cggcggggtc
     1861 cggctgcatc cgcaacccgt cgctggaccg tgccagaaag ttcagcttga ccgcgtactg
     1921 ctgcgcctcc acctcctcct gcatgggagt gggtggcggt ggcggagact cctgctttcg
     1981 cggccgccgg agaaaggaca tgcgctcccc taggtatcaa cgccgttcgg gctgctcggc
     2041 cgccgcgatt ctagcagcgg ggcgcccccc gaccggaata gtcccttcgg acccggctac
     2101 tggacgtcga gaccggccag gcgctcctcg agctcggcca agcgctcccg ctcgcgctgc
     2161 accacctgcg gcggagcctt ggccaggaag ccctcgctgg cgagcaggcc gcggacgcgc
     2221 ctgatctgct cctccagctc ggcgcgctgc ttctgggctc gctgcgcggc acgctgcacc
     2281 tccgccccgc ccgcctcgat ccaggcgtac cccagcgccg tcgccgcagc gtgctcgggg
     2341 aagtcgtggg tggccgcggc cagcaggatc tccaggggcc gtgcgcgcca cagctgtccg
     2401 aggtacggct ccgcctggcg caggatgtcc tctgcctgcg ccgagccgga ggccacccgg
     2461 acgctgcgcc actccccggc gggcagctgc tgttgcgtcc ggaggtcgcg tacctcacgg
     2521 gtggccgcaa tgacccgctg catggcatcc tccgcgccga cgtccctcgc ggcgggcgcc
     2581 ggccaggccg cggagatcag cagcctctcg ccggccgtgg cctccgcgtt cacctcgcgc
     2641 agcgtggccc agatctcctc ggtcacgaac ggcaggatgg gatgcagcag gcgcagcgtg
     2701 gcggccagca cgtcggccaa ggtcctccaa gtcctggccc gctcccctag gctcgcctcc
     2761 tcgcgtcgca ggtcgatctt ggcggtctcc aggaaccagt cgcagtagtc ggaccaggcg
     2821 aagtcgtaca cggtcgccgc atatccggcc aggtccagct cgtccagctg ccgcgtggcg
     2881 cgctcgatgg tggcggacaa ccgggagtgg atccagcgct ccggaagcgt ggcctcgccc
     2941 tccaccgggg cgagcggttc gggccgcgcg ccgagcacga agcgagcggc
//
LOCUS       DATACA010000206         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_910934, whole genome shotgun sequence.
ACCESSION   DATACA010000206 DATACA010000000
VERSION     DATACA010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910934"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VHK28_04315"
     CDS             complement(<1..646)
                     /locus_tag="VHK28_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2141487.1"
                     /translation="MIPETRYTRAGDIHIAHQVLGEGAVDVILADQWFSNMDAQWDVP
                     PLAELRRRLASFSRLIMFDRRGMGLSDPVAIQSLPSLDAWMDDLRAVMAAASSDRAAL
                     IANIGGAITSLVFAASHPDRLSSLVIVDGFARVRSAPDYPAGMPDAEVERELELIEPS
                     WGRGLMLDIFAPSMRGVPGLRETWARFERQATSPGSARAIVGWIFDTDVRTFLPT"
     gene            complement(686..1576)
                     /locus_tag="VHK28_04320"
     CDS             complement(686..1576)
                     /locus_tag="VHK28_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX2141488.1"
                     /translation="MHAPTARFLPRPSVDPVLLAAVVGAWALMLALIAADQAAVIEHD
                     AVLSGAGAAWPLALLLFVASWQLMTAAMMLPTSLPMVRAFVTAARTQPHPRGAVAAFL
                     AGYFAIWTAFALAALAGDAGLHWLVDRWSWLAERPWLISGGVLMGAGAFQFSPIKERC
                     LAECRSPIQFLWRHYRRGLGGAWNLGLRHGVFCLGCCWALMLVMFAVGVGSLAWMAGL
                     TGVMLLEKTSRFGRRLVPVVGGALMVWGALVLLRPGAAAAHAGEVHVEAAPLDQVIGG
                     ALVLVALGLVLAWRRRARAG"
     gene            complement(1581..2186)
                     /locus_tag="VHK28_04325"
     CDS             complement(1581..2186)
                     /locus_tag="VHK28_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX2141489.1"
                     /translation="MAYAVEGRLLEVCSCNVLCPCWIGEDPDGGTCDSALAWHIDDGQ
                     VDGVDVSGLTLALSVHIPGNVLKGNWKAAVLVDDQASDEQQEAMLKLFTGQLGGAIAD
                     LAGLIGEVVAVERVPITFTVEEGKGTFKLGSVASAELHGYAGATGKPTVMSETVFSTI
                     PGSPVYVAKSAHFERDGTEHGLPSVAISGRNALQGHFRFES"
     gene            complement(2306..>4237)
                     /locus_tag="VHK28_04330"
     CDS             complement(2306..>4237)
                     /locus_tag="VHK28_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2141490.1"
                     /translation="RPREYVFKHALIRDAAYDSMPRGRRARAHAAVGTWLEAVAKERL
                     AEVDEFVAEHYRRAVEEGDAAGIWATHADEREQLRARAFRRLVSAGAAARQRYALDRA
                     VELHAHAVQLATAPAERAEALEELGEDHETGLRGEEAMESYLGALTDARSPGVPAERR
                     AQICMKAARTLVLRWGAFPSRPDPVLMDQLLDEGMAATSDPVTRCWLLALAGGAAIRW
                     HSDVQAPDPVPFDERIRRTRLALDEAARIGQPDLAGFAARIVGQLQFEDRRFVESRET
                     MRSVRPLLSRMHSKFQRALTSMYVFLAVADVEGRYEDALALAAEMLELGREMSAHERM
                     HGTFGQLWALHHLGRWSEMGAIGEEHLEALQGEDQMVCPYVRSGPLVWALTLAYLGDG
                     QAVDRITSQIKPTWDEPGLPEILLSRIAIARGRHAEGRRLAEGILASGRSPSLEENAF
                     DRIAFIEALVALEDWSALGDAVLDARQWEPALALMKPYCDRADGLAALAAGNRGAGIT
                     RLRSADSHFGRLELRYEVARTKALLAQAMPNSSGLLTEAIESAEPLLGGTAPGTAPVT
                     LSVAAQERLTDREMEVLTRVAEGLDNDGIAGELAISVRTVERHLSNLYAKLGVGGKSA
                     RAAATARAYEANLIGRPPD"
BASE COUNT          656 a         1520 c         1468 g          593 t
ORIGIN      
        1 tggtaggtag gaaggtgcgc acgtcagtat cgaagatcca ccccacgatc gcccgcgcgc
       61 tgccggggct cgtggcctgg cgctcgaagc gcgcccacgt ctcgcgcagt ccaggcacgc
      121 cgcgcatgct cggcgcgaag atgtcgagca tcaggccacg gccccagctc ggctcgatca
      181 actcgagctc ccgctccacc tccgcgtcgg gcatcccggc cgggtagtcg ggcgccgacc
      241 ggacgcgggc gaagccgtcg acgatgacga gcgagctgag ccggtccggg tgcgatgcgg
      301 cgaacacgag gctggtgatg gccccaccga tgttcgcgat caatgccgcg cggtcgctgc
      361 tggccgcggc catgactgct cgcagatcgt ccatccaggc gtccagggac ggcagcgact
      421 ggatggccac cggatcggag aggcccatcc cgcgccgatc gaacatgatc agccggctga
      481 aggatgccag gcgccgccga agctcggcca gcggtggcac gtcccactgg gcatccatgt
      541 tgctgaacca ctgatccgcc aggattacgt cgacggcccc ctcaccgagc acctggtggg
      601 cgatgtggat gtcgccggca cgtgtgtagc gggtctccgg aatcaccgag gcaggatacg
      661 accccgcggc tttcgcgctc ggggactagc ccgctcgcgc tcgccgtcgc caggcgagga
      721 caagccccag cgctaccagc accagggccc ctccaatgac ctggtccagg ggcgccgctt
      781 cgacgtggac ctcaccggcg tgcgccgcgg cggcacccgg tcgcaacagg accagcgcgc
      841 cccaaaccat cagcgcaccg cccaccaccg ggaccagccg gcgcccgaag cggctggtct
      901 tctccagcag catcaccccg gtcaggcccg ccatccaggc caggctgccg accccgaccg
      961 cgaacatcac cagcatcagc gcccagcagc agcccaggca gaacacgccg tgtcgcagcc
     1021 cgaggttcca ggcgccgccc agcccgcggc ggtagtgccg ccacaggaac tggatcggtg
     1081 accggcactc cgcaaggcat cgctccttga tggggctgaa ctggaacgcg ccggcaccca
     1141 tcagcacccc gccgctgatc agccacgggc gctcggcgag ccagctccat cggtccacca
     1201 gccagtgcag gcccgcatcg ccggccagcg ccgccagggc gaatgccgtc cagatcgcga
     1261 agtatccggc caggaaggcc gccacagcgc cgcgtgggtg aggctgggtg cgcgccgccg
     1321 tcacgaaggc acgcaccatg gggaggctgg tcggcagcat catggccgcc gtcatcagct
     1381 gccagctggc cacgaacagc agcagggcca gtggccaggc cgcgccggcg ccggacagga
     1441 ccgcgtcgtg ctcgatcacg gctgcctggt cggcggcgat gagcgccagc atcagcgccc
     1501 acgccccgac caccgcagcc agcagcaccg ggtcgaccga gggacggggg agaaatcggg
     1561 cggtcggggc atgcatcggt ctaggactcg aagcggaagt ggccctggag ggcgttccgg
     1621 ccgctgatgg cgacgctggg caggccgtgc tcggtgccgt cgcgctcgaa gtgggctgac
     1681 tttgccacgt acaccgggga gcccgggatg gtgctgaaga cggtctcgct catcacggtg
     1741 ggcttgccgg tggcaccggc gtagccgtgc agctcggcgc tcgctacgga gcccagcttg
     1801 aaggtgccct tgccctcttc cacggtgaag gtgatgggaa cgcgctcgac ggccacgacc
     1861 tcaccgatga gcccggccag gtcggcgatg gcgcctccca gctgcccggt gaacagcttg
     1921 agcatggcct cctgctgctc gtcgctggcc tggtcgtcca ccaggacggc cgccttccag
     1981 ttgcccttca gcacgtttcc ggggatatgg accgatagcg ccagggtcag gccggagacg
     2041 tccacgccgt cgacctgccc gtcgtcgatg tgccaggcga gggcggagtc gcacgtcccg
     2101 ccgtcggggt cctcaccgat ccagcagggg cacaggacgt tgcagctgca gacctccagc
     2161 aggcgtcctt cgactgcata agccacgaat gtcgctcctt tcgtccgcgg ggccggccac
     2221 cagggccggc tggatgcgcg agacgatagg aacggcggtc cgcggccgca tgcgtgcacg
     2281 cacccatacg ctctggcacg gatggtcagt cgggtggtcg accgatcagg ttggcctcgt
     2341 aggctcgggc ggtggcagcc gcccgagccg acttgccacc gacacccagc ttggcgtaca
     2401 ggttggacag gtgccgctcc accgtccgga ccgagatggc cagctccccc gcgataccgt
     2461 cgttgtccag gccctcggcg acacgggtga ggacctccat ctcgcggtcg gtgagacgct
     2521 cctgggcggc gacggagagg gtgacgggcg ccgtgccggg ggctgttccg cccagcagcg
     2581 gctcggcgga ctcgatcgcc tcggtcagca gtcccgacga gttgggcatg gcctgggcca
     2641 gcagggcctt ggtgcgcgcc acctcgtagc ggagctcgag ccggccgaag tgggagtccg
     2701 cgctccgcag ccgggtgatt cccgcaccac ggttgccggc agcgagggcc gccaggccat
     2761 cggcccggtc gcagtacggt ttcatcagtg ccagcgccgg ctcccactgg cgggcatcaa
     2821 gcaccgcgtc cccgagtgcc gaccagtcct cgagggccac cagcgcttcg atgaaggcga
     2881 tcctgtcaaa ggcgttctct tccaggctgg ggctgcgccc agacgcgagg atgccctccg
     2941 ccagccggcg accttcggcg tggcgccccc gcgcgatcgc gatacgtgac agcaggatct
     3001 ccggcaggcc cggctcgtcc caggtgggct tgatctggct ggtgatgcga tccacggcct
     3061 gtccgtctcc caggtaggcc agagtcagcg cccacaccag cggcccgctg cgcacgtagg
     3121 ggcagaccat ctggtcctct ccctggagag cctccaggtg ctcttcgccg atggctccca
     3181 tctccgacca gcgcccgaga tggtgcaggg cccacagctg gccgaaggtg ccgtgcatgc
     3241 gctcgtgcgc gctcatctcg cgccccagct cgagcatctc ggctgcgaga gccagcgcgt
     3301 cctcgtagcg cccttccacg tcggcaaccg ccaggaagac gtacatcgag gtgagcgcgc
     3361 gctggaactt tgagtgcatc ctggagagca gcggacggac cgagcgcatc gtctcgcgcg
     3421 actcgacgaa gcggcgatcc tcgaactgga gctggcccac gatgcgggca gcgaagccgg
     3481 ccaggtccgg ttgaccgatg cgggcagcct cgtccagcgc cagccgcgtt cgacggatgc
     3541 gctcgtcgaa cggcaccggg tcgggagcct gcacgtccga gtgccaccgg atggcggcac
     3601 cgccggcgag ggccaggagc cagcatcggg tgaccggatc gctggtggca gccatccctt
     3661 cgtccagcag ttgatccatg agcacagggt cggggcggct gggaaaagct ccccagcgaa
     3721 ggacaagggt gcgtgccgcc ttcatgcaga tctgtgcgcg gcgctcggca gggactcccg
     3781 gggagcgggc gtcggtcagt gccccgaggt agctctccat cgcctcctcg ccgcgcagcc
     3841 cggtctcgtg gtcctctccg agctcctcga gtgcctcggc acgctcggct ggagccgtgg
     3901 ccagctggac ggcgtgtgcg tggagctcca cggcgcgatc cagcgcgtag cgctgccggg
     3961 ctgccgcacc ggcggagacc aggcgccgga aggcgcgggc gcgcagctgc tctcgttcgt
     4021 cggcatgcgt ggcccaaatg ccggccgcgt caccctcctc aaccgcccgg cggtagtgct
     4081 ccgcaacgaa ctcgtccacc tcggcaaggc gctccttggc gaccgcctcc agccaggtgc
     4141 cgacggccgc atgcgctcgt gctcgccggc cgcggggcat ggagtcgtag gccgcatcgc
     4201 ggatcagggc gtgcttgaag acgtactccc gcggccg
//
LOCUS       DATACA010000207         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_991316, whole genome shotgun sequence.
ACCESSION   DATACA010000207 DATACA010000000
VERSION     DATACA010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991316"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VHK28_04335"
     CDS             <1..577
                     /locus_tag="VHK28_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2141491.1"
                     /translation="SYLPDVLDPDEVARILDAPPADEPAGIRDRAILELLYACGLRVS
                     ELTGLDTDRLDLPHLQLRAIGKGIRERRVPMGEEARERLHRYLAGPRAEWTAGRPTPA
                     VFVSQRGTRLARESVWRLVRRWRDETGVRGHVTPHTFRHSFATHLLAGGADLRVVQAL
                     LGHASISTTQLYTHLTGERLREVYARAHPRA"
     gene            594..1409
                     /locus_tag="VHK28_04340"
     CDS             594..1409
                     /locus_tag="VHK28_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX2141492.1"
                     /translation="MSYARNLLSRGEEVVFESRQHWFAVLAETWLFVVIAVLSLAVLL
                     WASTGMEASGIRDVLQIASAVALLGSIVLIGLKVWAWRNQEYLITTRRVIKAEGIFNK
                     TMGDSSLEKVNDAHLSQSWIGRIFDFGTLDILTAAEEGGGVIDDFPMLAEPVKFKIAM
                     LNMKEQLEFPDLARPPAQRPNPVPSMQRAEPMPPRAGSDRVSEVRSEPMPEAAPVSAP
                     APAPSPVPSAPPPAPANVDQLSATLESLAGLRDKGLITPEEYEAKKREILERM"
     gene            1410..2294
                     /locus_tag="VHK28_04345"
     CDS             1410..2294
                     /locus_tag="VHK28_04345"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2141493.1"
                     /translation="MEVRRGGLPDGLYGAELERRILETAGAVRDRLAAEPSLASPQLA
                     VVLGSGLGGVVDLLRPEPRLTIPYSEIPNVPGSEVAGHAGELVAGLAAGRAALLLSGR
                     AHGYEGWSHRESTLLLRAVLSLGARSVVLTNAAGGVNPEYEPGDVMLISDVINMSGAN
                     PLTGPNLDRFGPRFLAMTDAMDADLRARARAAAQRAGVALREGVYLMLSGPSYETRAE
                     LRMLRTLGADAVGMSTVHEVIVARHMAARVLGFSLVTNKATADMEGEVTHEEVLAMGP
                     IGAARLVSVLRELLPELG"
     gene            2295..2933
                     /locus_tag="VHK28_04350"
     CDS             2295..2933
                     /locus_tag="VHK28_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2141494.1"
                     /translation="MLFGNPDPAQLIGVLVALVVGITFHEFSHAFVADSLGDHRTRAL
                     GRVSLNPLRHLDPIGSLFILIAGFGWGRPVPVNVYALRPGPIGMSWVSAAGPVANLVV
                     ATAFAVVFRAADVAGLVSSDNLFLANVLYAVVLYNVALALFNLLPIPPLDGYNLVLPL
                     LPPRIAYQVQQYAQYGMLVLLALVALSFYGGFSPLRWVFQLAAGIAGLLIGA"
     gene            2926..3408
                     /locus_tag="VHK28_04355"
     CDS             2926..3408
                     /locus_tag="VHK28_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141495.1"
                     /translation="MHRVEQFFGHLTARVTADETALARQILPAAAWPLFAGMPVADRR
                     HALDVVHRLLAAGWDDAELLSAALLHDAAKGRRMRLWHRVAGVLLEDFAPRLLAGLAS
                     ADEGSWRHAFYLYLHHAEISAAAALEAGCSPRTAALIRGTPGDADAQQAALRTADEAS
                     "
     gene            3416..>3822
                     /locus_tag="VHK28_04360"
     CDS             3416..>3822
                     /locus_tag="VHK28_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX2141496.1"
                     /translation="MPLEPIAIHPEVAPVATLPAAFELELDVFSGPLELLLQLIESRQ
                     LDVLTVPLAEMADAYVAHLATHPVPPQQLAEFVAIASQLILLKSRRLLPGEPMPLASD
                     PEAEPDEEELRRRLVEYRALRDVARLLGERDGQV"
BASE COUNT          512 a         1389 c         1333 g          588 t
ORIGIN      
        1 cagctacctg ccggacgtgc tcgaccccga cgaggtggcg cgtatcctgg acgcgccgcc
       61 ggcggacgag ccggccggga tccgggaccg ggccatcctg gagctgctct acgcgtgcgg
      121 tcttcgggtc agcgagctca ctggcctgga caccgatcgg ctggacctgc cgcacctcca
      181 gctgcgggcc atcggcaagg gaatccgcga gcggcgggtg ccgatggggg aggaggcgcg
      241 tgaacggctc catcggtacc tggccggccc gcgcgccgaa tggaccgccg ggcggccgac
      301 gccagccgtc ttcgtcagcc agcgcgggac gcggctggcg cgtgaatcgg tctggcgcct
      361 ggtgcggcgc tggagggacg agacgggtgt ccgcgggcac gtcacgccgc acaccttccg
      421 gcacagcttc gccacccatc tgctggccgg tggcgctgac ctgagagtgg tccaggcgct
      481 cctcggccat gctagcatca gcaccaccca gctctacacg cacctgaccg gggagcgact
      541 gcgagaggtc tacgccagag cgcacccgag agcctgagca gcggagcacg ccgatgagct
      601 acgccaggaa cctcctgagc cggggcgagg aagtcgtctt cgagagccgc cagcactggt
      661 tcgccgtgct cgccgagacg tggctcttcg tcgtcatcgc cgtcctgtcc ctggccgtcc
      721 tgctgtgggc gtccacgggc atggaggcca gcggcatcag ggacgttctc cagatcgcgt
      781 ccgcggtcgc cctcctggga tcgatcgtcc tcatcggtct caaggtgtgg gcgtggcgca
      841 accaggagta cctgatcacc acccgacgcg tgatcaaggc ggagggcatc ttcaacaaga
      901 ccatgggcga ctcgagcctg gagaaggtca acgacgcgca cctctcgcag agctggatcg
      961 gccgcatctt cgacttcggg acgctcgaca tcctgaccgc ggccgaggag ggcggcgggg
     1021 tcatcgacga cttcccgatg ctggccgagc cggtgaagtt caagatcgcc atgctcaaca
     1081 tgaaggagca gctcgagttc ccggacctgg cccggccgcc cgcgcagcgg cccaacccgg
     1141 tgccgtccat gcagcgcgcc gagccgatgc cgccgcgcgc cggctccgac cgggtcagcg
     1201 aggtccgctc cgagcccatg ccggaggcgg caccggtctc cgcgcccgcg ccggccccga
     1261 gcccggttcc ctcggccccg ccaccggcgc cggccaacgt cgaccagctg tccgccaccc
     1321 tcgagagcct cgccggcctg cgcgacaagg gactgatcac cccagaggag tacgaggcca
     1381 agaagcgcga gatcctggag cggatgtagg tggaggtccg ccgaggcggg ctgcccgacg
     1441 ggctctacgg ggccgagctg gagcgccgca tcctggagac ggccggcgcc gtgcgtgatc
     1501 ggctggccgc ggaaccctcg ctcgccagcc cgcagcttgc cgtggtgctc ggctccgggc
     1561 tgggtggggt cgtcgacctg ctgcgccccg agccgcggct caccatcccg tactcggaga
     1621 ttcccaacgt tcccggatcg gaggtggccg ggcacgccgg cgagctggtg gccggcctgg
     1681 ccgccggccg ggccgccctg ctcctctcgg ggcgagcgca cggctacgag ggctggagcc
     1741 accgcgagtc gacgctcctg ttgcgcgcgg tcctgtcgtt gggcgcccgg agcgtggtcc
     1801 tcaccaacgc cgctggcggc gtgaaccccg agtacgagcc gggcgacgtg atgctcatct
     1861 ccgacgtgat caacatgtcc ggcgccaatc cactcaccgg tcccaacctg gaccgattcg
     1921 ggccgcgctt cctggccatg accgatgcca tggacgcgga cctgcgggcc cgcgcccggg
     1981 cagcggcgca gcgagccggc gtcgcgttgc gcgaaggcgt gtacctgatg ctctccggac
     2041 cgagctacga gacccgcgcc gagctgcgca tgctccgcac gctgggggcg gacgcggtcg
     2101 gcatgtccac cgtgcacgag gtgatcgtgg cgcgccacat ggccgcccgg gtgctgggct
     2161 tcagcctggt caccaacaag gcaacagcgg atatggaggg cgaggtcacg cacgaggagg
     2221 tgctggccat ggggcccatc ggtgccgcca ggctggtctc cgtcctccgc gagctgctcc
     2281 cggagctggg ctagatgctc ttcggcaatc ccgatccggc gcagctcatc ggcgtgctgg
     2341 tggcgctcgt ggtgggcatc accttccacg agttcagcca cgcgttcgtg gcggacagcc
     2401 tcggcgatca ccggacgcgt gcgctgggac gggtgagcct caacccgctg cgccacctgg
     2461 acccgatcgg ctcgctcttc atcctgatcg ccggattcgg ctggggtcgc ccggtgccgg
     2521 tcaacgtata cgccctgcga cccggcccga tcgggatgtc gtgggtctcc gccgccgggc
     2581 cggtcgccaa cctggtggtg gccaccgcct tcgccgtcgt cttccgcgcc gcggacgtgg
     2641 ccggcctcgt ctcgtccgac aacctgttcc tggccaacgt cctgtacgcg gtggtgctgt
     2701 acaacgtggc cctcgccctg ttcaacctgc ttcccatccc gccgctggat ggctataacc
     2761 tggtgctgcc gctcctgccg ccgcgcatcg cctaccaggt gcagcagtac gcccagtacg
     2821 ggatgttggt gctcctggca ttggtcgcgc tctccttcta tggcggcttc agcccgctgc
     2881 gctgggtgtt ccagctggcg gccgggatcg ccgggctgct gatcggtgca tagggtcgag
     2941 cagttcttcg gccacctgac cgctcgcgtc acggccgatg agaccgcgct ggcgcggcag
     3001 atcctgcccg cggcggcctg gccgctcttc gccggcatgc cggtcgccga tcggcgccac
     3061 gccctggacg tggtccaccg gctgctggcg gccggctggg atgatgccga gctgctctcc
     3121 gcggcgctgc tgcacgacgc ggccaaggga cgtcgcatgc gcctctggca ccgggtcgcc
     3181 ggcgtgctgc tggaggactt cgcaccgcgg ttgctggcag ggctggcctc ggctgacgag
     3241 ggctcgtggc gccacgcctt ctacctctac ctccaccacg ccgaaatttc ggcggcggcg
     3301 gcgctcgagg ccggctgctc gccacgcacc gcggccctta tccgagggac ccccggcgac
     3361 gccgatgcgc agcaggccgc gctgcgtacc gccgacgagg catcctgagc gcgtcatgcc
     3421 gctcgagccg atcgccatcc accccgaagt cgctcccgta gccacgctgc cggcagcctt
     3481 cgagctggag ctggatgtct tcagcggccc gctcgagctg ctgctgcagc tgatcgagtc
     3541 gcgccagctg gacgtcctga ccgtgccgct ggcggagatg gccgatgcct acgttgcgca
     3601 cctggccact cacccggtgc cgccccagca gctcgccgag ttcgtggcca tcgcctccca
     3661 gctcatcctg ctgaagtcgc gccgcctgct tcccggggag ccgatgcccc tggcgtccga
     3721 tccagaggcg gagccggacg aggaggagct gcgccgtcgc ctggtcgagt accgggcgct
     3781 gcgagacgtg gcccgcctgc tcggcgagcg cgatggccag gt
//
LOCUS       DATACA010000208         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1071427, whole genome shotgun sequence.
ACCESSION   DATACA010000208 DATACA010000000
VERSION     DATACA010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071427"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VHK28_04365"
     CDS             <1..283
                     /locus_tag="VHK28_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2141497.1"
                     /translation="VAEETGWRVVGEPRLIHVVDWDEVRGDPSTGRREFDFLVEVDGD
                     LATPRLEWPKQVEWRWLGRDDLDLLDENRGADDGVVRRIVEIALERAGR"
     gene            601..1518
                     /locus_tag="VHK28_04370"
     CDS             601..1518
                     /locus_tag="VHK28_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2141498.1"
                     /translation="MRVEYREEPCRTALNKVSGMPFRWSLNPYMGCVHQCTFCYVRGF
                     ELRAGRPADARYGTSIRVKTNIVEVLRTQLAKRTWKRETVAIGSATDPYQPAEGRYRL
                     TRSCLVALAEGNTPFNLITRGPLIVRDLDVLTDATPRVDVSITFSVPTLDERIWRTTE
                     PGTAPPSQRLRALRTLSDAGIKVGVGMAPILPGLSDDPRLLRDVVIAARDAGAHHLWW
                     NVVNLRPGTREHFLENLARDWPEQLDRYQRLFARGAYLGKDATAQIDAALTALRSEWP
                     MRPIRRPGRADQPPLRPPGSQQLELPIAG"
     gene            complement(1515..>1987)
                     /locus_tag="VHK28_04375"
     CDS             complement(1515..>1987)
                     /locus_tag="VHK28_04375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141499.1"
                     /translation="AARAGVTEHIRRARPEEADALRELAHRSKAHWPYSAEFLERVRP
                     MLQLTPADVREHEVWVLEIDGQVAGWHRVTFERGRAELEDLWLEPAEIGSGLGRVLFE
                     HAVAVARRAGATAMEWDAEPYAEGFYRAMGGQEIGRSPSAAEEGRTLPRMRLDL"
BASE COUNT          298 a          713 c          673 g          303 t
ORIGIN      
        1 ggtggccgag gagaccggct ggcgggtggt cggcgagccg cggctgatcc acgtggtcga
       61 ctgggacgag gtgcgcggcg atccatccac cgggcgccgc gagttcgact tcctggtgga
      121 ggtggacggt gacctggcca cgcctcgcct ggagtggccg aaacaggtcg aatggcgctg
      181 gctcgggcgc gatgacctcg atttgctcga cgaaaaccgc ggggccgacg acggcgtcgt
      241 gcgacgaatc gtggagattg ccctcgaacg cgcggggcgt tgagagtgcc ctcgtcgagc
      301 ggagggctga gggttgacgg cgcgacctgg ccatatcccg ccgcacgccg gccataaccg
      361 cccaatggct cgatgttctc ggtcacagcc cgcccggcat cggtcttagc cagttggggg
      421 gcccgggtct gagcgtgacg cgtgctcagg caggcggctg gcgtctggct taaacggccc
      481 cggccggccc tttggcagac catcggccgg caagcagctt gtgaggcagc catttggcgg
      541 gatctggcag ggaagcgacc actgcaagta tagaacgcac gttctatact cccctacctg
      601 atgcgcgtcg agtaccgcga ggaaccgtgc cgcacggcat tgaacaaggt gagcggcatg
      661 ccgtttcgct ggtcgctgaa cccgtacatg ggctgcgtgc accagtgcac gttctgctac
      721 gttcgcggct tcgagctgcg cgctggccgg ccggctgacg cccgctacgg cacctccatc
      781 cgggtcaaga ccaatattgt cgaggtcctg cgcacgcagc tggcgaagcg aacgtggaag
      841 cgcgagacgg tggccatcgg ttccgcgacc gatccctacc agccagccga gggccgatat
      901 cggctcaccc gcagctgcct ggtggcgctg gcggagggaa acacgccctt caacctgatc
      961 acccgcggcc cactgatcgt gcgcgacctg gacgtcctga ccgatgccac cccgcgggtg
     1021 gacgtctcca tcaccttctc ggtccccact ttggacgagc ggatctggcg cacgaccgaa
     1081 cccggcaccg cgcccccgag ccagcgcctg cgtgcgctgc gcacgttgtc tgacgccgga
     1141 atcaaggtcg gcgtcgggat ggcgcccatc ctgcccgggc tgtcggacga ccctcggctg
     1201 ctgcgggacg tcgtgatcgc cgcgcgcgac gccggggcgc accacctgtg gtggaacgtc
     1261 gtcaaccttc gaccgggcac gcgcgagcac ttcttggaga acctggcccg cgactggccg
     1321 gagcagttgg accgatacca gcggctcttc gcgcgtggcg cgtacctggg caaggacgcc
     1381 acagctcaga tcgacgctgc gctgacggcc ctccgctcgg agtggccgat gcgccccatc
     1441 cgccgccccg gccgtgccga ccagccgccc ctgcgaccac ccggttcgca gcagctggag
     1501 ctgccgatcg cgggctagag gtccaggcgc atgcggggca gcgtgcggcc ctcctcggcc
     1561 gccgacggcg atcgcccgat ctcctggccg cccatggccc ggtagaagcc ctccgcgtag
     1621 ggctcggcgt cccactccat ggcggtcgca cccgcccgcc gggcgacggc aacggcgtgc
     1681 tcgaacagca cccgcccgag gccggatccg atctccgccg gctccagcca taggtcctcc
     1741 agttcggccc tgccgcgctc gaaggtgacc cgatgccaac cggcgacctg cccgtcgatt
     1801 tccagcaccc acacctcgtg ctcgcgaacg tccgcgggcg tcagctgcag catcggccgc
     1861 acccgctcca gaaactccgc gctgtacggc cagtgcgcct tggaccgatg cgccagctcg
     1921 cgaagcgcat cggcctcctc cggtcgggcg cgtcggatgt gctcggtcac ccctgcgcgt
     1981 gcagcat
//
LOCUS       DATACA010000209         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_831015, whole genome shotgun sequence.
ACCESSION   DATACA010000209 DATACA010000000
VERSION     DATACA010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831015"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VHK28_04380"
     CDS             <1..909
                     /locus_tag="VHK28_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2141500.1"
                     /translation="LVRAVRFAVRYRLALDPATAAAIQRNAEAAASLSGERVRDELLR
                     MLRFPAPSAALALMEDLGLLTVLLPELAALREVEQDKALPGDALDHSLRTMDALPADD
                     PVLRLAGLLHDLGKATTLAGGHFIGHEEEGVGLAGRLLHRLRMSNADIVRVTRLIRHH
                     MFNYGREWTDAAVRRFVRRVGGDLLDDLLALRVADNAASGAVEGADGGVAELERRARQ
                     VLAGDALEQRQLAVNGHDLVAGLGLRPGPEVGRILDRLMDAVLDDPALNSRERLLALA
                     RGELAASGESGAAVHREGDGAAGAPG"
     gene            986..1744
                     /gene="deoC"
                     /locus_tag="VHK28_04385"
     CDS             986..1744
                     /gene="deoC"
                     /locus_tag="VHK28_04385"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX2141501.1"
                     /translation="MTAIGRRAARLEHRLTRPNASLSEVDAAVALAIEHECAAVTVSP
                     WLVKAAKRALGRSRLRLGTLVGHPHGTQILAVKAFEASKALEQGANQVDFVLNGGALV
                     SGEDEVVYNDMLAVIDMAHSALAVAGVVIESDPLPDDLLRRACLLAERAGADYVVSSA
                     GEMPARRTIQRVALLRHGVGPRVQVKAAGRFRTLADLRAAANAGASLIATALTPQLAQ
                     AAMAAAGSAEPATAGGAASSSRASAEPVAGARTA"
     gene            1768..>2498
                     /gene="galE"
                     /locus_tag="VHK28_04390"
     CDS             1768..>2498
                     /gene="galE"
                     /locus_tag="VHK28_04390"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX2141502.1"
                     /translation="MRILVVGGAGYIGSTSSEAFAQAGHEVTVYDDLSTGHQDAVLDP
                     AVLIRGSIQDKAHLESVLRDRRIDAVLHCAAKSLVGESMVEPGLYYRHNVSGGVAMLE
                     AMRGVGVTRLVYSSTAAVYGEPRRVPIAESDRTEPINPYGATKLAFEGAMRWFAAAHG
                     FQAISLRYFNVAGATERNGEDHDPETHLIPLVLRVAAGEAGHVQIFGQDYPTPDGTCI
                     RDFIHVRDLAAAHLLALEATGDADPS"
BASE COUNT          347 a          886 c          926 g          339 t
ORIGIN      
        1 cttgttcgcg cggtgcgctt cgcagtccgg taccggctgg cgctggatcc ggccacggca
       61 gccgccatcc agcgcaacgc ggaggcggcg gcctcgctct ccggcgagcg ggtgcgcgac
      121 gagctcctgc gcatgctccg cttcccggcc ccgtcggcgg cgctggccct gatggaggat
      181 ctcggcctgc tgaccgtgct gctgccggag ctggccgcgc tgcgcgaagt ggagcaggac
      241 aaggcgctgc ccggcgatgc cctcgaccac tcgctgcgga ccatggacgc gctgccggcc
      301 gacgacccgg tgctccgcct ggccgggctg ctgcacgacc tgggcaaggc aaccaccctg
      361 gcgggcggcc acttcatcgg tcacgaggag gagggagtgg ggctggcggg ccggctgctg
      421 catcggctgc ggatgtccaa cgccgacatc gtgcgggtga cgcgcctgat ccggcaccac
      481 atgttcaact acggccgcga atggaccgac gcggcggtgc gccgcttcgt gcggcgagtg
      541 gggggcgacc tgctcgacga cctcctggcg ctgcgggttg ctgacaacgc cgccagtggg
      601 gcggtcgagg gggccgatgg aggcgttgcc gagctggaac ggcgcgcgcg gcaggtgctc
      661 gccggcgacg cgctggagca gcggcagctg gccgtcaacg ggcacgatct ggtggcaggc
      721 cttggccttc ggccggggcc ggaggtgggc cgcatcctcg accgactgat ggatgccgtg
      781 ctggacgacc ccgcactgaa cagtcgcgag cggctgctgg cgctggctcg cggggagctg
      841 gcggccagcg gtgagagtgg tgcagcagtg caccgagaag gtgatggcgc ggctggggca
      901 cccggataag gcgctgctac aatcggccgt ccggagccat gcccttccgc tccgagcccg
      961 gccacaagga ggtcccctag ccgcgatgac cgccatcggg cgccgagctg cgcgcctgga
     1021 gcaccgcctg acgaggccca acgcgtcgct ctccgaggtg gacgccgcgg tcgccctggc
     1081 catcgagcac gagtgcgccg cggtcaccgt cagtccctgg ctggtcaagg cggccaagcg
     1141 tgcgctcggc cgatcacggc tccggctcgg gacgctggtc ggccacccgc acggcaccca
     1201 gatcctggcg gtcaaggcct tcgaggccag caaggcgctg gagcagggcg ccaaccaggt
     1261 cgatttcgtg ctcaacggcg gggcgctggt gtcgggcgag gacgaggtcg tctacaacga
     1321 catgctggcg gtgatcgaca tggcccactc ggcgctggcg gtggccggag tggtgatcga
     1381 gtccgacccg ctgccggacg acctgctgcg gcgtgcctgc ctgctcgccg agcgggcggg
     1441 agcggactac gtggtctcct cagccgggga gatgccggcg cgacgcacca tccagcgcgt
     1501 ggcactgctg cgccatggcg tcgggccgcg ggtgcaggtc aaggcggccg gccgattccg
     1561 gaccctggcc gacctgcggg cggcggccaa cgccggcgcg tcgctgatcg ccaccgccct
     1621 gaccccgcag ctggcacagg ccgccatggc ggcggccggg tcggccgagc cggcgaccgc
     1681 aggcggagca gcgtcctcga gcagggcatc ggccgagccg gtggccggcg cccgcacggc
     1741 ctagcacgag agggaggagg cgttcgattg cggatcctgg tcgtcggcgg tgccggctac
     1801 atcggctcca ccagcagcga ggccttcgcc caggcggggc acgaggtcac ggtctacgac
     1861 gacctctcca ccgggcacca ggacgccgtg ctggacccgg cggtcctgat ccgcggctcg
     1921 atccaggata aggcccacct cgaatccgtc cttcgtgacc gacgcatcga cgccgtcctg
     1981 cactgcgccg ccaagtccct ggtcggcgag tcgatggtcg agccgggcct ctactaccgg
     2041 cacaacgtgt cgggcggggt ggccatgctg gaggcaatgc gtggcgtcgg cgtaacgcgc
     2101 ctggtctact cgtccaccgc cgcggtctac ggagagccgc gccgggtgcc catcgccgaa
     2161 tcggacagga ccgagcccat caacccctac ggcgccacca agctcgcgtt cgagggcgcc
     2221 atgcgctggt tcgccgcggc gcacggcttc caggccatca gcctgcgcta cttcaacgtg
     2281 gcaggcgcga ccgagcgcaa cggcgaggat cacgaccctg agacgcacct catcccgctg
     2341 gtcctgcgcg tggcggccgg cgaggcgggc cacgtccaga tcttcgggca ggactacccg
     2401 accccggatg gaacctgcat tcgcgacttc atccacgtcc gcgacctggc cgccgcgcac
     2461 ctgctggcgc tggaggcgac cggcgacgcc gatccctc
//
LOCUS       DATACA010000210         4157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2437226, whole genome shotgun sequence.
ACCESSION   DATACA010000210 DATACA010000000
VERSION     DATACA010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437226"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1089
                     /locus_tag="VHK28_04395"
     CDS             103..1089
                     /locus_tag="VHK28_04395"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012295042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2141503.1"
                     /translation="MAELNILEAIRTAMDEEMERDERVMVLGEDVGRKGGVFGATDGL
                     WAKYGDKRVLDTPLTEAMIAGAAAGAAIYGLRPIAEMQFADFAYPAFNQIVSEISRFH
                     YRSNGTFSVPMVIRMPYGGGVHGALYHSQSNEAYFTSTVGLKVVAPGTPADAKGLLKA
                     AVRDPDPVMFFEHKKTYRLFRAEVPDGDGVVPIGKAEVARPGKDLSIFCYGFMRHCAL
                     EAAEILAREDGLEAEVVDLRTLRPLDTATVLASVEKTAKALVVHEANRFGGFGAEVAA
                     TIAEQGFEHLDGPVTRLAGPEVPGVPYHHDLEEWFMVGPARIVEAARKVAAY"
     gene            1216..2751
                     /locus_tag="VHK28_04400"
     CDS             1216..2751
                     /locus_tag="VHK28_04400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX2141504.1"
                     /translation="MATTIKMPQLGESVTEGTIERWLVKEGDTVQQYDPLFEVVTDKV
                     NAEVPAEVAGTITKIIVGDGETVKVGTPLAEIASDGDGAQPAAETAAETTTAQPAPEQ
                     VEAKPAQPEMPAQPAAAAEPAAEAPELPQPEQPAPQAEPQPAQQPAAAQAQPSGEAPA
                     PATATDASMRMTPAVRRLVREHDVDVSQLRGSGAGGRITRDDVLSFVQSGGAAAQAPA
                     AQAPAPEAPPAAPQQPEPAAPAPAAQPAPQPAPQPAPQAQAAPTPSYQAPQPVAAPSG
                     GDQEVPLSQMRKGIATKMTRVKQTVPHAYTIVEVDMHNVVRWRTANQEAYKAREGIGL
                     SYVAVVVKAVTETLRQHPTLNSQFAEDKIVLKQAMNIGIAVAVENGLLVPVIQNADQL
                     SISGVNHRVQDLAARARSNKLKLDEIQGGTFTVNNTGWFGSVASMPIINAPEVAILSM
                     EAIVKRPVVIEVDGEDIVAIRPMMNMTCSFDHRVLDGAQVGAFMADVKKRLESWDLDT
                     PIG"
     gene            complement(2757..3503)
                     /locus_tag="VHK28_04405"
     CDS             complement(2757..3503)
                     /locus_tag="VHK28_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX2141505.1"
                     /translation="MTGGHETPEEAASSLERALRAVGTRDRAVHEKRYLKSDLDFLGA
                     SVGQIERAVRTWTAELPSPPGHDELLLLADALWRRPVHERRMAAVMLLERHVRLLTPA
                     DLCFVERLLRESRTWALVDGISTDVAGAILAADPEGTRSVLDRWATDADFWVRRASLL
                     SELRPLRRGAPLEPFLRRADAMLDEREFFIRKAIGWVLREVGKRRPTEVVAWLEPRIT
                     RASGVTVREAVKYLPDPDRERLLAGYRARR"
     gene            complement(3513..3689)
                     /locus_tag="VHK28_04410"
     CDS             complement(3513..3689)
                     /locus_tag="VHK28_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141506.1"
                     /translation="MDIPPFATLVTSQIWIVPAVVFGGPGLLVILFMALQLAGALAWL
                     PAVRRLRGEEHPAD"
BASE COUNT          661 a         1476 c         1493 g          527 t
ORIGIN      
        1 cgcgcccgga gcccagcgtc gcggaggccg agcgccgggt gtacgccgaa ccgattcccg
       61 gcggcggtac tcccgacgag acgccagcgg agggaggctg acgtggccga gctgaacatc
      121 ctcgaggcca ttcgcacggc catggacgag gagatggagc gcgacgagcg ggtgatggtc
      181 ctgggcgagg acgtggggcg caagggtggt gtcttcggcg ccaccgacgg cctgtgggcc
      241 aagtacggcg acaagcgcgt gctcgacacg ccgctgacgg aggcgatgat cgccggcgcc
      301 gcggccgggg ccgccatcta tggtctgcga cccattgccg agatgcagtt cgccgacttc
      361 gcatacccgg ccttcaacca gatcgtcagc gagatcagcc gcttccacta ccggtcaaac
      421 ggcaccttct cggtccccat ggtgatccgc atgccgtacg gcggcggagt gcacggcgcg
      481 ctgtaccact cgcagtccaa cgaggcctac ttcacctcca cggtgggact caaggtggtc
      541 gcccccggca ccccggcgga tgcaaagggc ctgctcaagg ccgcagtccg cgatcccgac
      601 ccggtcatgt tcttcgagca caagaagacc taccgcctgt tccgcgccga ggtgccggac
      661 ggtgatggcg tggtgcccat cggcaaggcg gaggtggcgc gccccggcaa ggacctgtcc
      721 atcttctgct acggcttcat gcggcattgc gccctcgagg cggccgagat tttggcccgc
      781 gaggacgggc tggaggcgga ggtcgtcgac ctgcgcaccc tgcggccgct ggacaccgcc
      841 accgtgctgg cctcggtgga gaagacggcc aaggcgctgg tggtccacga ggccaaccgc
      901 ttcggcggct tcggcgcgga ggtggctgcc accatcgccg agcagggctt cgagcacctg
      961 gacgggccgg tcacccgcct ggccggcccc gaggtgcccg gcgtccccta ccaccacgac
     1021 ctggaggagt ggttcatggt cgggccagca cgcatcgtcg aggcggcgcg caaggtggcc
     1081 gcctactgat ggaccgatgc gtcccacgct cggtttcgca aagtaggccc cgagcggcta
     1141 aggtcaacca gacagtgagt tcgagacatc cggccggcgg caggccggcg cagcactagg
     1201 gagccggcaa cgacgatggc aaccacgatc aagatgcccc agcttggcga gtcggtgacc
     1261 gagggaacga tcgagcgctg gctggtcaag gaaggcgata ccgtccagca gtacgacccg
     1321 ctgttcgagg tcgtgaccga caaggtcaac gccgaggtgc ccgccgaggt ggccggcacg
     1381 atcaccaaga tcatcgtggg cgacggcgag acggtgaagg tcgggacccc gctggcggag
     1441 atcgccagtg atggcgatgg cgcgcagccg gccgccgaga ccgcggctga gaccacgacc
     1501 gcccagcctg cccccgagca ggtcgaggcg aagcccgctc agccggagat gccagcacag
     1561 cccgccgctg ccgccgagcc cgccgccgag gctcccgaac tgccgcagcc ggagcaaccg
     1621 gcgccccagg ccgagccgca gccggcgcag cagcccgcgg cggctcaggc tcagccgagc
     1681 ggcgaggcgc ccgcgccggc gaccgcgacc gacgcgtcga tgcgaatgac gcccgccgtt
     1741 cgacgcctgg tgcgcgagca cgacgtcgac gtcagccagc tgcgcggcag cggcgccggc
     1801 ggtcgcatca cgcgcgacga cgtgctgtcc ttcgtccagt ccggcggcgc ggcggcgcag
     1861 gcaccggctg cccaggcccc ggcgccggag gcacctccgg ccgctccgca gcagcccgag
     1921 cccgcggcac ccgcaccagc cgcacagccg gcgccccagc cggcgcccca gccggcgccg
     1981 caggcgcagg cagcgccaac cccgtcatac caggcgccgc aaccggtcgc cgcgcccagc
     2041 ggtggcgacc aggaggtgcc cctcagccag atgcgcaagg gcatcgccac caagatgacg
     2101 cgcgtcaagc agacggtgcc gcatgcctac accatcgtcg aggtcgacat gcacaacgtt
     2161 gtgcgctggc gcaccgcgaa ccaggaggcc tacaaggcgc gggagggcat cggtctcagc
     2221 tacgtggccg tcgtggtcaa ggcggtcacc gagacgctgc gacagcatcc gaccctcaac
     2281 agccagttcg ccgaggacaa gatcgtcctc aagcaggcca tgaacatcgg catcgcggtg
     2341 gcggtcgaga acgggctcct ggtaccggtc atccagaacg cggatcagct gtccatcagc
     2401 ggcgtcaacc accgcgtcca ggacctggcg gcgcgggctc gctcaaacaa gctgaagctg
     2461 gacgagatcc agggcggcac cttcacggtc aacaacaccg gctggttcgg ctccgtggcg
     2521 tccatgccga tcatcaacgc gcccgaggtg gcgatcctgt ccatggaggc catcgtgaag
     2581 cgcccggtgg tcatcgaggt ggacggcgag gacatcgtgg ccattcgccc gatgatgaac
     2641 atgacctgct ccttcgacca ccgcgtgctg gatggggcgc aggtgggcgc cttcatggcg
     2701 gatgtcaaga agcgcctgga gagctgggac ctcgatacgc cgatcggctg atcgcgtcag
     2761 cgccgcgcgc ggtacccggc cagcagccgc tcgcggtccg gatccgggag gtacttgacc
     2821 gcctcgcgca cggtcacgcc tgacgctcgg gtgatgcgtg gctcgagcca ggcgaccacc
     2881 tcggtcggcc ggcgcttgcc gacctcgcgc agcacccagc cgatcgcctt gcgaatgaag
     2941 aactccctct cgtccagcat ggcatctgct cgacgcagga acggctccag cggcgcgccg
     3001 cgccgcagcg gcctcagctc ggacagcagc gaggcccggc gcacccagaa gtcggcatcg
     3061 gtcgcccatc ggtccaagac gctccgggtg ccctccgggt cggcagccag gatggcgccg
     3121 gccacgtcgg tcgagatgcc gtcgaccagc gcccaggtcc gcgactcgcg caggaggcgc
     3181 tcgacgaagc acaggtccgc cggcgtcagc agtcgaacgt gacgctcgag gagcatgacg
     3241 gccgccatcc gccgctcgtg gaccggccgc cgccagaggg cgtccgccag gagcagcagc
     3301 tcgtcgtgcc cgggtggtga cggcagctcg gcggtccagg tcctgaccgc ccgctcgatc
     3361 tggccgaccg atgcacccag gaagtccagg tcgctcttga ggtagcgctt ctcgtgcacg
     3421 gcacgatctc gcgtgccgac cgcgcgcagc gcgcgctcca gcgagctcgc cgcctcctcg
     3481 ggagtctcgt ggccgcccgt cacccgcgga gtctagtccg cggggtgctc ctccccgcgc
     3541 agacggcgga cggcaggcag ccaggccagc gcgccggcga gctgcagtgc catgaacagg
     3601 atcaccagca ggcccggccc gccgaagacc acggcgggga cgatccagat ctgggaggtg
     3661 accagcgtgg cgaacggcgg gatgtccacc aggccgctgc ccgacccggc gtcgggttca
     3721 gcgacgcgga aaccggttcc gaaggggccg ggacccggtg ccggcggggg ttctccggcc
     3781 gcgcctccgc ctccggagcc gtcgcccacc ccgggagacg gcgagggatc gactggtgat
     3841 gccccggcgg tgggatcgga ccagttggga tgcgggggcg ccgtgggcga tgccagtgcc
     3901 acagccgagc gagtgggcac cggcgacggc gtcgctgagc gcggcctcgg cggcgcgctc
     3961 ggcgcggccg ttgctggtgc gctgggcttc ggcgttggcg tggcggttgg cccgggcgtt
     4021 gccgggtggt gggctggggc gtcggactct ctgcgacggt cggttgcggg gtgggcgtca
     4081 cggccgggcc gctcaccgcg acgaccggcg gcactcccag cagcgaggtg ctgccttcgc
     4141 agtcctggtc ccggtac
//
LOCUS       DATACA010000211         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2115798, whole genome shotgun sequence.
ACCESSION   DATACA010000211 DATACA010000000
VERSION     DATACA010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115798"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VHK28_04415"
     CDS             <1..1095
                     /locus_tag="VHK28_04415"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2141507.1"
                     /translation="AIRSGRASETRGYAVARERNRPPIRRILLVGVMLLLTTVVVGSV
                     LLWQRVSNFNDTVSTVGAASSRLFGPLNGDERVNVVLLGYGGPEHAGGNYLADSIQIL
                     SIDPQTDATTMIPIPRDLWVEGLAAMPDNGKINEGFAVGYQSSDGSIEEAARFTTEIL
                     SQITGLRIEHWMAIDFAGFREMVDAVGGVTVNNPREFSYTWSEPYFHAGNWTGGTFAA
                     GELHLNGQQALDYSRSRYTSVPQESSDFARSVRQQRVLSALRSKLGEGGLGSIGPGLA
                     MMDAMEGRMKTNLSAVDLFLLSGHINPDRRLELAEGVILEATSNSIGQYILVVIGRAH
                     GADYAPLQQFIATELAKPLPTPTPMPSAGP"
     gene            1207..3165
                     /gene="uvrC"
                     /locus_tag="VHK28_04420"
     CDS             1207..3165
                     /gene="uvrC"
                     /locus_tag="VHK28_04420"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX2141508.1"
                     /translation="MPRPEARDQHLRAILRQLPTSPGVYLMKGTSGRVLYVGKADSLR
                     HRVRSYFGSKAALDARILRMTQEVADVDYIVTDTISEAFLLEGNLIKEHRPRFNIRLR
                     DDKSYPFVKITLGEDFPRIVRTRKLARDGSRYFGPYASASSVDETLRLLRKIFPFRTC
                     NLDIPEGKRVLERPCLLYYIKRCQGPCIQAVEKDDYRATIARIVDFLEGKQEGIAAEL
                     RAEMQASSDGLRFEQAAAARDKLRAVERTIEQQKVAAFSRAEQDVVGMAREEGEACMQ
                     VLVIRNGKMIGREHFMLENATDVTDGEVLTSFLQQYYAATERPPREVLVPLAPAEADG
                     LAAFLAERRGSRVTVHVPERGEKRRLVALATQNAVEALAKERAEWLADAGKRDEALEQ
                     LAEALGLSRPPERIECYDMSNIQGTAAVGSMVVFVNGRPEPREYRRFRIRSGDTPDDF
                     RMMAEVLRRRFSRAARLRSETGALSLAAVGADEVPEEVDSDADGDERRPRGDGWSIPD
                     LVIVDGGKGQLSAALGAMTELGMTDVPLAGLAKRFEELYVPGRSAPIVLPRNSQGLYL
                     VQRIRDEAHRFAITYHRNVRSRRALASVFDEVAGIGPARRRALLKRFGSVRRIREASV
                     EEVASTPGIGREVAERLKAHLAREGMLA"
     gene            3438..4763
                     /gene="secD"
                     /locus_tag="VHK28_04425"
     CDS             3438..4763
                     /gene="secD"
                     /locus_tag="VHK28_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX2141509.1"
                     /translation="MTWRRIAPWVILITLILAIFISLPRQTLGLTGLPDFLGPNLRTV
                     LGLDLQGGVRVTLAATPLPGEEVTDEQLATARTIIERRVGGIGVSEPQVRTEVSGNQR
                     RIVVELPGVENEQQVRDLVGSTGQLQFIDPRGQTLREGQDITGLVTSGTVGVLFDGAS
                     IDPASVAPDNQTGRPGVVFNLRSPASDIWCSFTSANIGRPGPIALDGQVITTPNIDGA
                     ICGGTTVITVGGTGEEARAEQENLYNTLRFGALPVSLEEVGSERVEPTLGRGFLEQAI
                     VAGGIGLVLVMIFMIAFYRMPGVLAAFALVFYTLVVYAVFRLIGVTLTLAGVAAFILS
                     IGMAVDANILIFERMKEEIRAGKTLVPAIEAGFNRAWSSILDSNVSSILVAAWLYWQG
                     TTVVRGFALVLIIGVLVSMFSAVTVTRTVLRYVVRTDWGRRLELYHVER"
BASE COUNT          736 a         1639 c         1714 g          734 t
ORIGIN      
        1 gccatccgct ccggccgcgc ctccgagacg cgcggctacg ccgtggcgcg cgagcgcaac
       61 cggccgccga tccgccgcat cctgctcgtc ggggtgatgc tgctgctgac cacggtcgtg
      121 gtcgggtcgg tcctcctctg gcagcgggtc tcgaacttca atgacacggt gtccaccgtg
      181 ggggccgcct cgtcgcggct ctttggccct cttaacggcg acgagcgggt gaacgtggtg
      241 ctgctcgggt acggcgggcc cgagcacgcc ggcggcaact acctggcgga ctcgatccag
      301 atcctgtcga tcgatcccca gaccgacgcc accaccatga tccccattcc ccgcgatctg
      361 tgggtcgagg gactggctgc catgccggac aacggaaaga tcaacgaggg gttcgcggtc
      421 ggctaccagt ccagcgatgg cagcatcgag gaggcggcgc gcttcacgac cgagatcctc
      481 tcgcagatca cgggcctccg gatcgagcac tggatggcga tcgacttcgc cggcttccgc
      541 gagatggtgg acgcggtcgg gggcgtcacg gtcaacaacc cgcgggagtt cagctacacc
      601 tggagcgagc cgtacttcca cgccggcaac tggaccggcg gcaccttcgc cgccggtgag
      661 ctgcacctca acgggcagca ggccctggac tactcgcgct cgcggtacac gagcgttccg
      721 caggaatcaa gcgacttcgc ccggtcggtg cggcagcagc gcgtgctctc ggccctgcgc
      781 tccaagctgg gggagggggg actgggatcc atcggtccgg gcctggccat gatggacgcc
      841 atggagggcc ggatgaagac caacctgtcc gccgtggacc tgttcctgct gagcggccac
      901 atcaacccgg accgccgcct ggagctggcg gagggcgtga tcctggaggc caccagcaac
      961 agcatcggtc agtacatcct ggtggtgatc ggccgagcgc acggcgccga ctacgcgccg
     1021 ctccagcagt tcatcgccac cgagctggcc aagccgttgc cgacgcccac gccgatgccg
     1081 tccgccggcc cttgagggtt gccggaagcg ccaggtattc gcgaggcgaa tggatggcgc
     1141 cagaagccga ggcggagcgc ctgtcgtcgg gaggtggctc acgcgcccga agcctagaat
     1201 cggatcatgc cccgacccga ggcgcgcgac cagcacctgc gcgccatcct gagacagctc
     1261 ccgacctcgc cgggcgtcta cctgatgaag gggaccagcg gtcgcgtgct gtacgtgggc
     1321 aaggccgatt cgctgcgcca tcgggtgcgg tcctacttcg gctccaaggc cgcactggac
     1381 gcgcggatcc tgcgcatgac gcaggaggtg gccgatgtcg actacatcgt caccgacacc
     1441 atcagcgagg cgttcctcct ggagggcaac ctcatcaagg agcaccggcc gcgcttcaac
     1501 atccggttgc gcgacgacaa gagctacccg ttcgtgaaga tcacgctggg cgaggacttc
     1561 ccgcggatcg tgcgcacccg caagctggcg cgcgacggga gccgttactt cggtccgtac
     1621 gcgtccgcct cgagcgtcga cgagacgctg cgcctgcttc gcaagatctt cccgtttcgc
     1681 acctgcaacc tggacatccc ggagggcaag cgggtgctcg agcgcccgtg cctgctgtac
     1741 tacatcaagc gctgccaggg cccctgcatc caggccgtgg agaaggacga ctaccgcgcc
     1801 accatcgccc ggatcgtcga cttccttgag ggcaagcagg aggggatcgc ggccgagctg
     1861 cgtgccgaga tgcaggccag ctcggacggg ctgcgcttcg agcaggccgc cgcggcccgg
     1921 gacaagctgc gggcagtgga gcgcaccatc gagcagcaga aggtggcggc cttcagccgt
     1981 gccgagcagg acgtggtcgg catggcgcgc gaggaggggg aggcgtgcat gcaggtgctc
     2041 gtcatccgca acggcaagat gatcggtcgc gagcacttca tgctcgagaa cgccactgac
     2101 gtgaccgatg gagaggtcct gacctcgttc ctgcagcagt actacgccgc gaccgagcgg
     2161 ccgccgcgcg aggtgctggt tccgctggct cctgcggagg ccgatggact ggccgccttc
     2221 ctcgccgagc gccgcggctc acgcgtgacc gtccacgtgc cggagcgcgg cgagaagcgc
     2281 cggctggtgg ccctggccac ccagaacgcc gtcgaggcgc tggccaagga acgcgcggag
     2341 tggctggcgg atgccggcaa gcgggacgag gcgctggagc agctggccga ggcgctcggc
     2401 ctgtcgcgcc cgccggagcg gatcgagtgc tacgacatga gcaacatcca gggcaccgcc
     2461 gcggtgggct caatggtggt attcgtaaac ggccggccgg agccgcgcga gtaccggcgt
     2521 ttccgcatcc gctccggcga cacgccagac gacttccgga tgatggccga ggtgctgcgc
     2581 cgccgtttct cgcgtgccgc gcggctgcgg tccgagaccg gtgccctctc cctggcagcg
     2641 gtgggcgccg acgaggtacc ggaggaggtg gactccgacg ccgatggtga cgagcgccgg
     2701 ccgcgtggcg atggatggtc tatccccgac ctggtgatcg tggacggcgg gaaggggcag
     2761 ctgtcggcgg cccttggcgc catgaccgag ctgggaatga ccgacgtgcc gctggccggc
     2821 ctggcgaagc gcttcgagga gctctacgtt ccaggacgct cggcgccgat cgtcctgccg
     2881 cgcaactccc agggcctcta cctggtgcag cgcatccgcg acgaggcgca ccgcttcgcg
     2941 atcacctacc accgcaacgt tcgcagccgg cgggcgctgg ccagcgtctt tgacgaggtg
     3001 gccggcatcg ggccggcgag gcgacgggcg ttgctgaagc ggttcggatc ggtgcggcgc
     3061 atccgggagg catcggtcga ggaggtggcg tccacgccgg ggatcgggcg cgaggtggcc
     3121 gagcgcttga aggcgcacct ggcgcgggag ggaatgctgg cgtagggggc cccgggggct
     3181 gggcgagccg ccggcaggcg gtgtcagggg gcggtagtac ggcatggtta cacaggcgca
     3241 cagcggcacg gacgcacagc tgggcctgag gcgcgagggc ccggcgttcg agcgccgaca
     3301 gcgggtccgg gagtttgcgc cgcgcgtcgg ctcgaaccta taatcctgcc gcccgccgcc
     3361 gagtgcggcg tgtttcgtgt ctgcgccgct ccgcggacgc gcccgtcgcc cgagcgagag
     3421 gaccaaccgg ccatagcatg acctggcgcc gcatcgcccc gtgggtgatc ctcatcaccc
     3481 tgatcctggc gatcttcatc tccctgcctc ggcagacgct cggcctgacc ggcctgcccg
     3541 atttcctggg gccgaacctg cgcaccgtgc tgggcctcga cctgcagggc ggcgtgcggg
     3601 tgacgctggc tgccacgccg ctccccgggg aggaggtcac ggacgagcag ttggccacgg
     3661 cgcggacgat catcgagcgc cgagtcggcg gcatcggcgt cagcgagccg caggtgcgta
     3721 ccgaggtcag cggcaaccag cgccgcatcg tcgtcgagct gccgggcgtg gaaaacgagc
     3781 agcaggtacg cgacctggtg ggctccaccg gccagctgca gttcattgac ccccgcggcc
     3841 agacgctgag ggaagggcag gacatcacgg gcctcgtcac cagcggcacg gtcggggtgc
     3901 tgttcgacgg cgccagcatc gacccggcaa gcgtggcgcc cgacaaccag accggccgcc
     3961 cgggcgtcgt cttcaacctg cgttcaccgg cgtccgatat ctggtgctcg ttcaccagcg
     4021 ccaacatcgg ccggcccgga ccgatcgcgc tcgacggcca ggtcatcacc acgccgaaca
     4081 tcgatggggc gatctgcggc ggcaccaccg tgatcacggt ggggggcacc ggcgaggagg
     4141 cacgggccga gcaggagaac ctctacaaca ccctgcgctt cggcgcgttg cccgtctcgc
     4201 tcgaggaggt cggctccgag cgcgtggagc ccaccctcgg ccgcggcttc ctggagcagg
     4261 cgatcgtggc gggaggcatc ggtctcgtgc tggtcatgat cttcatgatc gccttctacc
     4321 gcatgccggg cgtgctggcc gcgttcgcgc tggtcttcta cacgctggtg gtgtacgccg
     4381 tcttccggct catcggtgtg accctgacgc tggccggggt ggccgccttc atcctgtcca
     4441 tcggcatggc cgtcgacgcc aacatcctca tcttcgagcg gatgaaggag gagatccgcg
     4501 ccggcaagac gctggtgccg gccatcgagg ccggcttcaa ccgcgcctgg tcgagcatcc
     4561 tggactcgaa cgtcagctcc atcctggtcg ccgcctggct ctactggcag ggcacgaccg
     4621 tggtgcgcgg cttcgccctg gtgctgatca tcggtgtgct ggtctccatg ttcagcgccg
     4681 tgacggtcac ccgcaccgtg ttgcgttacg tggtccgcac ggactggggc cgccgcctcg
     4741 agctgtacca cgtggagcgc tgagcgatgt gggacatcgt cggacaccgt cgctggtact
     4801 tcctcttcag cgccctgatc acc
//
LOCUS       DATACA010000212         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2356889, whole genome shotgun sequence.
ACCESSION   DATACA010000212 DATACA010000000
VERSION     DATACA010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356889"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..910)
                     /locus_tag="VHK28_04430"
     CDS             complement(377..910)
                     /locus_tag="VHK28_04430"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2141510.1"
                     /translation="MTDVPRVRLRDVTLSDADLLDEWANDPRDRGGFNDFGGAPRATP
                     REVLEKGPLRNERNGILIVERVEDGAPLGTVSWHRVNYGPSPQSDAWNCGIELIPEAR
                     GMGYGAEAQRLLARWLFDNSDLNRVEASTDVENVAEQRSLEKAGFTREGIQRGAQFRA
                     GAYHDLVTYAVIRSDLG"
     gene            complement(954..>3794)
                     /gene="mfd"
                     /locus_tag="VHK28_04435"
     CDS             complement(954..>3794)
                     /gene="mfd"
                     /locus_tag="VHK28_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX2141511.1"
                     /translation="GAADPLRVDLFGDEVESIRSFDPMTQGSRRRLEEAILLPASEFI
                     PAQGWEGLGSRVPVPRSEQLAADLAKLEHGDVGEAAETWAAVLTGAPAAAHVPEQAHL
                     VLTDSTELAAIANELDEQASQRRAGLVAAGELPGDWPLPYAAAETLRGLERRAAERLE
                     EGAAGDAGYRPAPVLPGRSERLAAWLGELVGSRHRIVVTTDQASRIGELLEEAGLPAG
                     AAAELRELPGPGGIGLVHGSLSVGFAHEPLELLVLTDRELFGATRVRRLVPAKRVVTR
                     DLIGKLSPGDHVVHVDHGIARFVGMTQREYGGDTKEYLQLDFAGDDKIFLPADQIGRI
                     TRYSGGAAPTLSKLGGTEWDRTKRRVRRAVAELAADLLEIYAAREAAPGFAFSPDTVW
                     QRELEDAFPYTETPDQARTIEEVKADMLRRRPMDRLVCGDVGYGKTEVALRAAFKAVQ
                     DGKQVAVLVPTTVLAQQHLGTFTRRLAPFPVRVEMLSRFVPKREQTRIVEGTADGSVD
                     ILIGTHRILSKDVAFADLGLLVVDEEQRFGVGHKERIKSMRREVDVLTLSATPIPRTL
                     HLSLVGIRDLSVIETPPEARLPIQTRIAEDDDALVRDAINRELDRGGQAFFVHNRVDT
                     IEAAAERVRRLIPRARVAIGHGQMAEGMLERVMLDFDSGKFDVLVCTTIIESGLDIPN
                     ANTIIISRADTFGLAQLYQLRGRVGRSDRRAYAYLLHRRGQPLSPVARKRLHAIFSAS
                     DLGAGYQIALSDLEIRGAGNILGAEQHGFMAAVGFELYTRMLAEAVDVRRGRVVGAEP
                     APVRLDLPGSAYLPDAYVGDAGAKLEMYRRFAQVRSEADAEALRAELLDRFGPVPDPV
                     EGLFRAVAVRMAAEAAHVPEVRAEARQVVFKWPRFDRRRVSMALELAGFRPSVGSNQV
                     RIPVGGGRNPVDVALRALAALAGAGEPAGVDA"
BASE COUNT          572 a         1381 c         1294 g          547 t
ORIGIN      
        1 cacggtgcgg tccagtcgcc caattaccaa tgccgatggc cgcccggtgg cctgggacgt
       61 aatcgcccgg tctgatgagc catccctcat cggctggggt ctcctcgcct acgtcatctg
      121 cgccgacgtg ggctgactcg ccgacaaaaa tgatggccgc ctccggggcc aacgacaacc
      181 ggaggcggtc aaagcctgac gccttcaatg ctcatcgatg ggtcagatcc gagcccgcgg
      241 ggtaacctga tcgatctatg aaggcgctga gcacgggaag gcacgaaaat cgcccgttcc
      301 tgcgcgccct gcccgcatag atcggtcagg cggcacgccg cagaggcttc gcgcgctgga
      361 tgcaccgata gatcggtcag cccaggtcgg agcggatcac cgcgtatgtc accaggtcgt
      421 ggtaggcccc cgcgcggaac tgtgcgccac gctggatgcc ctcacgggtg aacccggcct
      481 tctccagtga tcgctgctcg gcgacattct ccacgtcggt cgaggcctct acgcggttca
      541 ggtccgagtt gtcgaacagc cagcgcgcga gcaggcgttg ggcctcggcg ccgtagccca
      601 tcccgcgtgc ctctggaatg agctcgatcc cgcagttcca ggcatcagac tggggactgg
      661 gcccgtagtt gacgcggtgc cagctgaccg ttcccagcgg agcgccatcc tcgacccgct
      721 ccacgatcag gatgccgttc cgttcgttgc gcagcggccc cttctccagg acctcgcggg
      781 gcgtggcgcg tggcgcaccg ccgaagtcgt tgaagccgcc gcgatcgcgc gggtcgttgg
      841 cccactcgtc gagcagatcg gcatcggaca gggtgacgtc gcgcagccgg actcggggaa
      901 catcggtcat ggagttgcca gtatcgccga tggacccgat cccccgcgag ccgtcaggcg
      961 tcgactcccg caggttcccc cgccccggcc agggcagcca gcgcgcggag cgcaacgtcc
     1021 accgggttgc gtccaccgcc cacggggatc cgtacctggt tggaaccgac cgacgggcgg
     1081 aatccggcca gctccagcgc cattgacacg cgccgtcgat cgaagcgcgg ccacttgaac
     1141 acgacctgcc gcgcctcggc acgcacctcc ggcacgtggg ccgcctcagc tgccatgcgc
     1201 accgccacgg cccggaacaa cccctcgacc gggtccggca ccggcccgaa ccggtccagc
     1261 agctcggctc gcagtgcctc cgcgtccgcc tctgagcgga cctgggcgaa gcgccggtac
     1321 atctccagct tcgcaccggc atcgccgacg taggcatcgg gcaggtaggc cgagcccggc
     1381 aggtcgaggc gcacgggtgc cggctcggcg ccgaccaccc ggccgcggcg cacgtccacc
     1441 gcctcggcca gcatgcgcgt gtaaagctcg aagccgaccg ccgccatgaa gccgtgctgc
     1501 tccgccccca ggatgttccc cgcgccgcgg atctccaggt ccgagagcgc gatctgatag
     1561 ccggcgccca ggtcggaggc gctgaagatg gcatgcagcc gcttgcgggc caccggcgac
     1621 agtggctgac cccgccggtg caacaggtag gcgtacgccc gccggtcgga gcgcccgact
     1681 cgaccgcgca gctggtagag ctgcgccagc ccgaaggtat cggcccgact gatgatgatg
     1741 gtgttggcgt tcgggatgtc caggccggac tcgatgatgg tggtgcacac caggacatcg
     1801 aactttcccg agtcgaagtc gagcatcacc cgctcgagca tcccctccgc catctgtcca
     1861 tgaccgatgg ccacccgcgc gcgcgggatc aggcgccgca cccgctcggc ggccgcctcg
     1921 atggtgtcca cccggttgtg gacgaagaac gcctggccgc ctcgatccag ctcgcggttg
     1981 atggcgtcgc ggaccagcgc gtcatcgtcc tccgcgatgc gggtctggat cggcagccgc
     2041 gcctccgggg gcgtctcgat cacgctcagg tcgcgaatgc cgaccagcga caggtgcagg
     2101 gtgcgcggga tgggcgtggc cgacagggtc agcacgtcca cctcgcggcg catggacttg
     2161 atgcgctcct tgtgtcccac cccgaagcgc tgctcctcgt cgacgaccag cagccccagg
     2221 tcggcaaagg ccacgtcctt cgacaggatc cggtgcgtcc cgatcaggat gtcgacgctc
     2281 ccatccgcgg tgccctccac gatccgcgtc tgctcccgct tgggcacgaa gcgcgacagc
     2341 atctcgacgc gcaccgggaa gggcgccagc cggcgggtga aggttcccag gtgctgctgg
     2401 gccagcaccg tggtcggcac aagcacggcg acctgcttgc cgtcctgcac ggccttgaac
     2461 gcggcgcgca gcgcaacctc cgtcttgccg tacccgacgt cgccgcacac cagccggtcc
     2521 atgggccggc ggcgcagcat gtcggccttg acctcctcga tggtgcgcgc ttggtccggt
     2581 gtctcggtgt acgggaaggc gtcctccagc tctcgctgcc agacggtatc ggggctgaag
     2641 gcgaagccgg gagccgcctc gcgagccgcg tagatctcga gcaggtccgc ggcgagctcg
     2701 gcaaccgcac ggcgcacgcg gcgcttggtg cgatcccact cggtgccgcc cagcttggac
     2761 agcgtcgggg ccgcaccccc cgagtagcgg gtgatgcggc cgatctggtc ggccggcagg
     2821 aagatcttgt cgtcgccggc gaagtcgagc tgcaggtact ccttggtatc gccaccgtac
     2881 tcgcgctgcg tcatgcccac gaagcgggcg atgccatgat ccacgtgcac cacgtggtcg
     2941 ccggggctca gcttgccgat caggtcgcgg gtgaccaccc gcttggccgg gacgagacgg
     3001 cggacccggg tcgcgccgaa gagctcccgg tcggtgagca ccagcagctc cagcggttcg
     3061 tgcgcaaagc cgacggagag cgagccgtgc accagaccga tgcctccggg tcccggcagc
     3121 tcgcgcagct cggcggccgc cccggccggc agaccggcct cctccagcag ttcgccgatt
     3181 cggctggcct ggtcggtggt gaccacgatc cgatgccgcg acccgacaag ctcgccgagc
     3241 cacgcggcca gtcgctcgct gcggccgggg aggaccggcg cgggacgata tcccgcatcg
     3301 ccggcagcac cctcctcgag ccgctcggcg gctcgacgct ccaggccgcg cagcgtctcc
     3361 gccgcggcgt agggcagcgg ccagtcgccg ggaagctcac cggcagccac cagcccggcg
     3421 cgacgctggc tggcctgctc gtccagctcg ttggcgatgg cggccagctc cgtcgaatcg
     3481 gtgagaacca gatgggcctg ctccggcacg tgcgcagcgg ccggggcgcc ggtcaggacc
     3541 gcggcccacg tctcggcggc ctcgccgacg tcgccgtgct cgagcttggc caggtcggcg
     3601 gcgagctgct cggagcgcgg cacgggaacc cgtgacccga ggccctccca cccctgggca
     3661 gggatgaact cgctggccgg cagcaagatg gcctcctcga gccggcgccg gctgccctgg
     3721 gtcatgggat cgaaggagcg gatcgactcg acctcgtcgc cgaacaggtc cacgcgcagg
     3781 ggatcggcgg cgcc
//
LOCUS       DATACA010000213         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1554265, whole genome shotgun sequence.
ACCESSION   DATACA010000213 DATACA010000000
VERSION     DATACA010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554265"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VHK28_04440"
     CDS             <1..919
                     /locus_tag="VHK28_04440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2141512.1"
                     /translation="IAETYAQLAVSRTVRGDVNEALALTPSELRGLEVQSRLVPGTTL
                     IELIVRSPDPQLAAEYCGALGEGLIDYLGDVYEVFELVQIDPAGVPNAPVRPNVSLNL
                     LLGAGIALVAGVGVAFLAETIRSSRSGRAQTEIVDTESWAYNHTFFMLRLGEEMSRAR
                     RTDRPVAVGVMNVNHGRALEGLSPRQRAESMRRIATLLNTHVRPEDVVARLDESTFGL
                     LLVDTDEKEAMGMIEGIRARLSVPVLGASDTGDSIRVHPAAGLSTFRGESVSDIELVE
                     RARRALHQAELVPVGKTAAFSSIGASGAG"
     gene            924..2495
                     /locus_tag="VHK28_04445"
     CDS             924..2495
                     /locus_tag="VHK28_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX2141513.1"
                     /translation="MARSDVVEAAKGGGAVFAGRLFAWGSRFALAVFLARLLGSEEYG
                     LYNTALSLAAIGASFAVVGLDAALVRYASIFSARDDDAGLWGALQFGLALPALLGTAI
                     GIGVFIASEPIARDVFGVPTLEPLLRVMALLVPATVLNTQLAATLQGLKRIQYGVLAQ
                     QVWQPLVRFAVLVVMALIGMTAERAVIASVLATMAVTVLLLAMVGRFVPLGRPLSQAR
                     RQPGTLLRFSLPVYFSNVVTSLGSHLQAPLLGAMSSMASAGVFAVSSQINLVGSIFHQ
                     AVVSSSMPIFAELHDRGDRRGMEHLYQTTSKWTLSLNLPFFLAAVLFPNALLAIFGPE
                     FQAGAAALVILAWSNLVNAATGTSGALLDMTGYTVVKFVNSTVSVGLALALNLLLIPT
                     LGVVGAAIASLVAIGSVNALRLLEVRLLVHVQPYSAEYLKPLAAGAGAALAGALVLLT
                     TPGQGPLLQGAIGLPVVGAVYLVLLLRLGLTDDDRAILQRLRARFRRRGGGGRSAATG
                     RAAVTPGSSEPGNPS"
     gene            2492..2869
                     /locus_tag="VHK28_04450"
     CDS             2492..2869
                     /locus_tag="VHK28_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141514.1"
                     /translation="MSAQPTVVTKAPPPLAVTVRLFDVLEERAVRYCLWKSTTTLDVA
                     LAGRTDLDLLVHRSDAVALDAVARDLGFKPFLSHESRRFPGVEDLLGYDDESGRLVHL
                     HVYYQLVLGQQYVKNHHLPLSSS"
BASE COUNT          378 a         1019 c         1009 g          511 t
ORIGIN      
        1 gattgcggag acctacgccc agctggccgt aagccgcacc gtgcgcggcg acgtcaacga
       61 ggccctggca ctgacgccga gcgagcttcg cggcctcgag gtgcagagcc ggctggttcc
      121 ggggacgacg ctcattgagc tgatcgtgcg ttcgcccgac ccgcagcttg ccgccgagta
      181 ctgcggcgcc ctcggcgagg gcctgatcga ctacctgggc gatgtatacg aggtgttcga
      241 gctggtccag attgatccgg ccggagtgcc taacgccccg gttcggccca acgtgtcgct
      301 caacctgctg ctgggtgcgg gcatcgcgct ggtcgcaggg gtgggggtcg cgttcctggc
      361 cgagaccatc cgctcctcgc gctcggggcg cgcccagacg gagatcgtcg acaccgagtc
      421 gtgggcgtac aaccacacgt tcttcatgtt gcggctgggt gaggagatga gccgtgcgcg
      481 ccgcaccgat cggccggtgg cggtgggcgt catgaacgtc aaccacggac gcgcgctgga
      541 gggactgagc ccacgacagc gggccgagtc catgcggcgc atcgccaccc tgctcaacac
      601 ccacgtccgg cccgaggacg tggtcgcgcg actggacgag tccaccttcg gactgctgct
      661 ggtggacacc gacgagaagg aggccatggg catgatcgag ggaattcgtg cccgcctcag
      721 cgttccggtg ctcggcgcgt cggataccgg cgactccatc cgggttcatc ccgcagccgg
      781 gctcagcacc ttcaggggcg aatccgtgtc cgacatcgag ctcgtggagc gtgcccgacg
      841 ggcgctgcac caggccgagc tcgtcccggt cggcaagacc gccgcattct cgtcgatcgg
      901 cgcctccggc gccggctgag ccagtggctc gcagcgacgt ggtcgaggcc gccaagggtg
      961 gcggagcggt cttcgccggc cgtctcttcg cgtggggcag ccgcttcgcc ctggccgtgt
     1021 tcctggcgcg gctgctgggc agcgaggagt acggcctcta caacacggcc ctgtcgctgg
     1081 cggccatcgg tgccagcttc gcggtagttg gcctcgacgc cgcgctggtg cgctacgcgt
     1141 cgatcttctc ggcgcgtgac gacgacgctg ggctgtgggg cgcgctccag ttcggtctcg
     1201 ccctgccggc cctgctgggg acggccatcg gcatcggtgt cttcatcgcg tcggagccga
     1261 tcgcgcgcga cgtgttcggc gtcccgacac tggagccgct gctgcgcgtg atggccctcc
     1321 tggtgccggc caccgtgctc aacacgcagc tcgccgcgac cctgcagggg ctgaaacgca
     1381 tccagtacgg cgtcctggcc cagcaggtct ggcagccgct ggtgcggttc gcggtgctgg
     1441 tggtcatggc gctgatcggc atgaccgcgg agcgagcggt gatcgcttcg gtgctggcca
     1501 ccatggccgt cacggtgctg ctgctggcca tggtcggccg cttcgtcccc ctgggccggc
     1561 cgctgtccca ggcgcgccgg cagccgggga cgctgctgcg cttctcgctt cccgtgtact
     1621 tctcgaacgt cgtgacctcg ctcggctcgc acctccaggc cccgctgctc ggcgccatga
     1681 gctcgatggc ctcggccggc gtgttcgccg tctcgagcca gatcaacctg gtgggctcca
     1741 tcttccacca ggccgtggtc agctcctcga tgccgatctt cgccgagctc cacgaccgtg
     1801 gcgaccggcg cgggatggag cacctgtacc agacgacgtc gaaatggacg ctcagcctga
     1861 accttccgtt cttcctggcg gcagtcctgt tcccgaatgc cctgctggcg atcttcggtc
     1921 cagagttcca ggccggcgcc gcggcgctcg tgatcctcgc ctggtcgaac ctggtcaatg
     1981 cggccaccgg cacctcgggc gcactgctgg acatgaccgg ctataccgtg gtgaagttcg
     2041 tgaactcgac ggtctcggtc gggctggcgc tcgccctgaa cctgctgctc attcccaccc
     2101 tgggggtggt cggtgcggcc atcgcctcgt tggtggccat cggctcggtg aacgccctcc
     2161 gcttgctgga ggtgcggctg ctggttcacg tgcagcccta ctcggcggag tacctcaagc
     2221 ccctggccgc cggcgcggga gcggcgctgg ccggcgcgct ggtcctgctc accactccgg
     2281 gccagggccc gctgctgcag ggagccatcg gcctcccggt ggtgggcgcc gtgtacctgg
     2341 tcctgctgct caggcttggc ctgaccgatg acgaccgagc gatcctgcag cggctccgcg
     2401 cccgattccg gcggcgcggt ggcggggggc gctcggcggc caccggccgg gcagctgtca
     2461 cgccgggcag ctccgagccg ggaaatccgt cgtgagcgcg cagcccaccg tcgtgaccaa
     2521 ggctccgccg ccgctggcgg tgaccgtccg gctctttgat gtcctggagg agcgcgccgt
     2581 ccgttactgc ctctggaaga gcacgaccac gctcgacgtt gccctggcgg gacggacgga
     2641 cctggatttg ttggtgcacc gatccgacgc ggtcgccctc gatgccgtag cgcgcgacct
     2701 cggcttcaag ccgttcctgt cacacgagag ccggcgcttc ccaggagtgg aggatttgct
     2761 cggctacgat gatgagagcg gccggctggt ccacctccac gtctactacc agctggtgct
     2821 cggccagcag tacgtcaaga accaccacct gccgctctca tcatcgtagc cgagcaaatc
     2881 ctccactcct gggaagcgcc ggctctcgtg tgacagg
//
LOCUS       DATACA010000214         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_751058, whole genome shotgun sequence.
ACCESSION   DATACA010000214 DATACA010000000
VERSION     DATACA010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751058"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VHK28_04455"
     CDS             <1..1119
                     /locus_tag="VHK28_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX2141515.1"
                     /translation="RAAESTRKIVVANLMGADPTALGDRALAEANPHLVLEGVLIAAF
                     ATGASEAILAVRRDWPLAITRLEAAIQQAEAAHLAGYLVMGTDFSCTVIVREGSGALV
                     AGEESALLASLAGDRGMPVIRPPYPTEVGLWGAPTIVQNAETLAHAAWIVAHSAEAYG
                     TVGTASAPGTRLVSIYGKVGEPGVVEVPLGTPLSEVISLAGGSVGVTKAVIVGGPSGG
                     ALSAGELSTPYEYDALRAAGGGIGLGSMLVLDTETCMVATARFFLDWSAREACGKAVP
                     CRIGTKRLVEALDRVLAATPRPNDFVLMRELSRKMADTALCALEARAPNPLLTTMDRF
                     AEEYRAHAERGECLAGACDTQPVTPLAEPLPGLEPATT"
     gene            1181..>2491
                     /locus_tag="VHK28_04460"
     CDS             1181..>2491
                     /locus_tag="VHK28_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2141516.1"
                     /translation="MSFANGHRNGNGSGNGAGALRPEDYAPVWPMVPGTRKISVTING
                     EQVEAEEGQTILQACRVLGIDIPTLCYEPKLAPYGACRICVVEVEGSDDTPISCGTKI
                     AEGMVITTHSERLIENRRMVLELIFSDHDAYCLPPCQFKCPTRVDIPGYLKQNSLGNW
                     EEATRILKRSLPFPAILGRVCPAPCETHCRREEVDTAIAIRDSHRYCADRVLEVDAPA
                     PIPWPKDPDTGRRVAVIGAGPAGLSAAYYLQLRGHHCDVFEAEPEQGGMLRYGIPEYR
                     LPKGWMDRELDHVWELGATLKPNMRLGRDFHLADLLEQGYDAVYIGIGCYNPNELGIP
                     GEDAEGMVNALENLYNSTRGLPIPALRDARVVVIGGGFTAIDCTRTSLRQGSAEVTLV
                     YRRDLKDMPAQDEVHEAIEEGARVIFQASPVRILTDDRNRVTGVE"
BASE COUNT          379 a          894 c          879 g          339 t
ORIGIN      
        1 cgcgcggcgg agagcacgcg caagatcgtg gtcgccaacc tgatgggcgc cgaccccacc
       61 gccctcggcg acagggcgct ggccgaggcg aacccgcacc tggtgctgga gggcgtgctg
      121 atcgccgcct tcgccaccgg cgcctcggag gcgatcctgg cggtgcgccg cgactggccg
      181 ctggccatca cccgcctcga ggcggccatc cagcaggccg aggcagcgca cctggccggg
      241 tacctggtga tgggcaccga cttcagctgc acggtcattg tccgcgaggg atccggggca
      301 ctggtggccg gcgaggagtc ggcgctgctg gcctccctgg ccggcgaccg cggcatgccg
      361 gtcatccgcc cgccgtaccc caccgaggtt gggctgtggg gcgccccgac catcgtgcag
      421 aacgcggaga cgctggccca cgcggcctgg atcgtggccc actccgcgga ggcctatggc
      481 acggtcggga cagcgtcggc gcccggcact cggctggtca gtatctacgg caaggtgggc
      541 gagccggggg tggtcgaggt accgctcggc acgccgctga gcgaggtcat cagcctggcc
      601 ggcggatcgg tgggcgtgac caaagcggtc atcgtcggcg gaccgtcggg cggggccctc
      661 tcggccgggg agctgtcgac gccctacgag tacgacgcgc tccgggcagc cggcggaggc
      721 atcggtctgg gatccatgct ggtgctcgac accgagacct gcatggtggc cacggcgcgc
      781 ttcttcctcg actggtctgc ccgcgaggcc tgcggcaagg cggtcccctg ccggatcggc
      841 accaagcgcc tggtggaggc gttggaccgg gtgctggccg ccaccccgcg cccgaacgac
      901 ttcgtgctga tgcgcgagct gtcgcgcaag atggccgaca ctgcgctttg cgcgctggag
      961 gcgcgtgcgc cgaacccgct gctgaccacc atggaccgct tcgcggagga gtatcgggcg
     1021 cacgccgagc gcggcgagtg cctggccggt gcctgcgaca cgcagccggt cacaccactc
     1081 gcggagccac tccccggcct ggagccggcg acgacgtgat cggcttcgtg accgcttctg
     1141 cgagccaacc cggcagactc cgctagcatt caagggaccc atgagcttcg ccaacggaca
     1201 ccgcaacgga aacggatcgg gcaacggcgc cggcgcgctg cggccggagg actacgcgcc
     1261 cgtgtggccc atggttcccg gcacccgcaa gatcagcgtc accatcaacg gcgagcaggt
     1321 ggaggcggag gagggccaga ccatcctcca ggcctgccgg gtgctgggca tcgacatccc
     1381 taccctgtgc tacgagccca agctggcgcc gtacggggcc tgccgcatct gcgtggtgga
     1441 ggtagagggc tccgacgaca cgcccatcag ctgcggcacc aagatcgccg aggggatggt
     1501 gatcaccacc cattccgagc ggctgatcga gaaccgccgc atggtgctgg agctcatctt
     1561 cagtgaccac gacgcctact gcctgccgcc ctgccagttc aagtgcccga cccgggtcga
     1621 catccccggc tacctgaagc agaactcgct gggcaactgg gaggaggcga cccgcatcct
     1681 gaagcgctcg ctgcccttcc ccgccatcct ggggcgcgtc tgcccggcgc cgtgcgagac
     1741 gcactgccgg cgcgaggagg tggacaccgc catcgccatc cgcgactcgc accgctactg
     1801 cgcggaccgc gtgctggagg tcgacgcacc ggctcccatc ccgtggccca aggacccgga
     1861 caccgggcgg cgggtggcgg ttatcggcgc cggaccggcc ggcctctccg cggcctacta
     1921 ccttcagctg cgcggccacc actgcgacgt gttcgaggcc gagccggagc agggcggcat
     1981 gctgcgctac gggatccctg agtaccgcct gccaaagggc tggatggacc gcgagctgga
     2041 ccacgtctgg gagctgggcg ccaccctcaa gccgaacatg cgactgggcc gcgacttcca
     2101 cctggccgac ctgctggagc agggctacga cgcggtctac atcggcatcg gttgctacaa
     2161 cccgaacgag ctgggcatcc cgggcgagga cgccgagggc atggtcaacg cgctggagaa
     2221 cctctacaac tcgacccgcg gcctgcccat cccggccctg cgcgacgcgc gggtggtggt
     2281 catcggcggc ggctttaccg ccatcgactg cacgcgcacc agcctgcgcc agggctccgc
     2341 cgaggtgacc ctggtctacc gccgcgacct caaggacatg cccgcccagg acgaggtgca
     2401 cgaggccatc gaggagggcg cgcgggtcat cttccaggcc tcgccggtgc gcatcctgac
     2461 cgatgaccgc aatcgggtca ccggcgtcga g
//
LOCUS       DATACA010000215         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2277572, whole genome shotgun sequence.
ACCESSION   DATACA010000215 DATACA010000000
VERSION     DATACA010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277572"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..643
                     /gene="rpsD"
                     /locus_tag="VHK28_04465"
     CDS             44..643
                     /gene="rpsD"
                     /locus_tag="VHK28_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2141517.1"
                     /translation="MTRTPRHKTSRRFGVDIYGTDGASLRNRLGTPPGGVKQGRRRRP
                     SVYGEQLAEKQKARAIYGVRERQFRRYFADASRAEGPPGENLLVMLERRLDNVVYRLG
                     WARTRPMARQLVGHGHVRVDGKRVDIPSFLVKPGQRVELTEDAARIPAVVEEMAAGRP
                     IPAWLERDGERGGHVMRLPERADVDVPVDEAQIVGYYSR"
     gene            726..1418
                     /locus_tag="VHK28_04470"
     CDS             726..1418
                     /locus_tag="VHK28_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX2141518.1"
                     /translation="MERIERLLDGPATFLAVVFVLALAAELVLDAQGERVPGILSWTQ
                     TAIWVFFGIQFALGILVSPDRLRYLRRHWITAISLVLPFFRILRILRAARALRALSGF
                     RALSAFNRAARSLAAVLAWSRAGYPLALAGIAAVLGSATLFLFEADAAGSRIASYSEA
                     LWWTLSTLTTIGANSEPVTLGGRIVGLGVMVCGLVILGYVAGVVGALLFERRSRRRPA
                     GGAASAPGQLDR"
     gene            complement(1392..2498)
                     /locus_tag="VHK28_04475"
     CDS             complement(1392..2498)
                     /locus_tag="VHK28_04475"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2141519.1"
                     /translation="MTRPALPEELLRRAPKVLLHDHLDGGLRPATVIELAREAGYREL
                     PTEDPHELGAWFTRGADRKSLELYLEGFRHTVAVMQTRDAIERVAAEAVEDLAADGVV
                     YAEIRMAPELLVEGGLSLDEATEAMLAGLRRGAEDRPITAGLIVTAMRQAARSVEIAE
                     LAVRHRDGGVIGFDIAGPEAGFPPSRHLDAFHLIARENFHFTIHAGEGFGLPSIWEAL
                     QWCGAERLGHGVRIVDDISVRDDGRVTLGRLASYVRDRRIPLEMCPTSNVHTGAAPSV
                     AEHPIDLLRRLRFRVTVNTDNRLMSGITMSSEFGALAEAFGIGLEEMEWLTLNAMKSA
                     FWPFDGRLRIINEQIKPGYAQLRAELSAVELSRG"
     gene            2560..>2880
                     /locus_tag="VHK28_04480"
     CDS             2560..>2880
                     /locus_tag="VHK28_04480"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX2141520.1"
                     /translation="MTSDMPWELDRRPLIVAAATPLRDGGLELDEAAIWPMVRFLTGH
                     GADGIFACGTTGEGILLSTDERMRTAVLFRAALTDGILVVHCGTQTTAGTQELAAHAA
                     EIGAD"
BASE COUNT          388 a         1048 c         1013 g          431 t
ORIGIN      
        1 accgactgag cgtggcacgg ccgatgctcc ccagagcacc gccatgaccc gcacgccgcg
       61 tcacaagacc agccgccgct tcggggtcga catctacggc accgatggcg cctcgctgcg
      121 caaccggctc ggcacaccgc cgggtggcgt caagcagggg cgccgcaggc gcccatcggt
      181 ctacggtgag cagctggcag agaagcagaa ggcgcgggcc atctacggcg ttcgtgagcg
      241 ccagttccgg cgttacttcg cggacgcgtc gcgcgccgag ggcccgcccg gggagaacct
      301 gctggtcatg ctcgagcgac ggctggacaa cgtcgtctac cgcctggggt gggcgcgcac
      361 caggcccatg gcccggcagc tggtcgggca cgggcacgtg cgggtggacg gcaagcgggt
      421 cgatattccg tccttcctgg tgaagcccgg ccagcgcgtg gagctgaccg aggacgcagc
      481 gcgcatcccg gcggtcgtgg aggagatggc cgccggacgc cccattcccg cctggctcga
      541 gcgtgatggc gaacgcggcg gccatgtgat gaggctgccg gagcgggcgg acgtagacgt
      601 ccccgtggac gaggcccaga ttgtcggcta ctactcccgc tgaggcatcc ggttcgcggc
      661 ggccggcctt gcggtcccgg ccgccggatg agcggcaggc cacccgccgg gaacggtacc
      721 ggctggtgga gcgcatcgag cggctcctgg atgggccggc caccttcctg gcggtcgtct
      781 tcgtgctggc gttggccgcc gagctggtcc tcgacgcgca gggcgagcgc gtaccgggca
      841 tcctgtcctg gacgcagacc gccatctggg tcttcttcgg catccagttc gcgctcggga
      901 tcctggtctc gcccgatcgg ctgcggtatc tgcgacggca ctggatcacc gcgatctcgc
      961 tggtgctgcc cttcttccgc atcctgcgca tcctgcgcgc cgcccgcgcg ctgcgtgccc
     1021 tgagcgggtt ccgcgccctc agcgccttca accgggcagc gcggtcgctg gccgcggtcc
     1081 tggcctggag ccgggcaggc tatccgctgg cgctggccgg gatcgccgcg gtcctgggct
     1141 cggccacgct cttcctcttc gaggccgatg ctgccggcag ccgcatcgcc tcctacagcg
     1201 aggcgctgtg gtggacgttg tccaccctga ccacgatcgg ggcgaacagc gaaccggtga
     1261 cgctgggcgg gcggatcgtg ggcctcggcg tcatggtctg cggcctggtg atcctgggct
     1321 acgtcgccgg cgtggtgggt gccctgctct tcgagcgccg ctcccggcga cggcccgccg
     1381 gcggtgcggc gtcagccccg ggacagctcg accgctgaca gctccgcccg gagctgcgcg
     1441 tagccgggct tgatctgctc gttgatgatc cgcagccggc cgtcgaacgg ccagaaggct
     1501 gacttcatgg cgttcagcgt cagccactcc atctcctcca ggccgatgcc gaaggcctcc
     1561 gcaagcgcgc caaactcgct cgacatcgtg atgccgctca tcaggcggtt gtcggtgttg
     1621 accgtgaccc ggaaccgaag gcggcgcagc aggtcgatgg gatgctcggc cacggacggc
     1681 gccgcgccgg tgtgcacgtt cgaggttggg cacatctcga gcgggatgcg acggtcccgc
     1741 acgtacgacg ccagccggcc gagggtcacg cggccgtcgt cgcgcacgct gatgtcgtcc
     1801 acgatccgca ccccgtggcc caggcgctcg gcgccgcacc actgcagcgc ttcccagatc
     1861 gacggcagcc cgaatccctc cccggcgtgg atggtgaagt ggaagttctc gcgggcgatc
     1921 aggtggaagg cgtccaggtg acggctgggc gggaacccgg cctccggccc ggcgatgtcg
     1981 aagccgataa ctcccccgtc ccggtggcgc accgccagct ccgcgatctc caccgaccgc
     2041 gctgcctggc gcatggcggt cacgatcaac cccgccgtga tcggccggtc ctcggcgccg
     2101 cgccgaaggc cggccagcat ggcctcggtc gcctcatcca ggctcagccc gccctccacc
     2161 agcagctcgg gcgccatgcg gatctcggcg tagaccaccc cgtcggccgc aaggtcctcg
     2221 accgcctcgg cggcgacccg ctcgatggca tcgcgtgtct gcatgaccgc caccgtgtgc
     2281 cggaacccct ccaggtagag ctccaggctc ttgcggtccg cgccgcgcgt gaaccacgcc
     2341 cccagctcgt gcgggtcctc ggtggggagc tcgcggtaac ccgcctcgcg cgccagctcg
     2401 atcaccgtcg ccggccgcag tccaccatcg aggtggtcgt gcagaaggac cttcggcgcg
     2461 cgacgcagca gctcttcggg cagtgcggga cgggtcatca ccatggtggc gggcagccta
     2521 cccggcgggg caggtgcgcg acaaggtaac ctccgggacg tgacgtctga catgccctgg
     2581 gagctggacc gacgaccact gatcgtggcg gctgccacgc cgctgcgcga cggcgggctg
     2641 gagctggacg aagccgccat ctggcccatg gttcgcttcc tcacggggca cggcgcggat
     2701 ggcatcttcg cctgcggaac caccggcgag ggcatcctcc tgtcgaccga tgagcgcatg
     2761 cgcaccgccg tcctcttccg cgcggcgctc acggacggca tcctggtcgt ccactgcggc
     2821 acccagacca ccgctggcac gcaggagctc gccgcccacg ccgcagagat cggggccgat
//
LOCUS       DATACA010000216         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_590376, whole genome shotgun sequence.
ACCESSION   DATACA010000216 DATACA010000000
VERSION     DATACA010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590376"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VHK28_04485"
     CDS             complement(<1..457)
                     /locus_tag="VHK28_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141521.1"
                     /translation="MLPPLILLALAALLLSPGLFVGPSLDPAVFVHVASELRNGEALY
                     VGTWDHKPPGIFLLYAAAQAVLPFVDPWVVAWVLSVIATAATGWLVAWIGRRMGVTSG
                     IALLAGSGAVIAMAQYLTALGGGLTEPIASVPLVAALAFAVAEGGVRRVV"
     gene            527..1366
                     /locus_tag="VHK28_04490"
     CDS             527..1366
                     /locus_tag="VHK28_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2141522.1"
                     /translation="MQRLAQRVVIGLAFAYALVGVYLIIGSWGFEDVDAYWDAAMRLR
                     DGEPLYPPGQDPDNYRVYRYAPWFAWLWVPLTYLPRAAVEIGWGLLVGVAGAAVLGGL
                     FRLRTAAAAALALLLAPWMLSLVQVGNIQPLVVAMLGFGISRRSGPLWVGIAASLKAV
                     PALWALVYVARGEWPRLVVAGAVGAGLTATFLLYDRTGYVTDPGRSGMSLHYYFGPVA
                     WLIGAVASAGVAVVLAWRRSDWLWPAVAVAAMLVAPRSHITYATFLAIGLLNGARDRI
                     DAR"
     gene            1356..2249
                     /locus_tag="VHK28_04495"
     CDS             1356..2249
                     /locus_tag="VHK28_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2141523.1"
                     /translation="MRAEAVARLAAVVIAGFGAVILIGAGWIPGIKPDADAYWLAAIR
                     LREGEPLYLPVSAYANEIDIYRYAPWFAYAWVPLTYLPQESAYLVWRALLLGGAVAAV
                     VPILRRLTPAGLVLALLILSLLVSNLPAANVTALMVGALAVSLRTRAGPVVLGLAGSL
                     KIFPLLLVAGYVAERRWRDVAIALGVAAVLWAHLLAFGVEDYPTEFGGVGLYRAAAWL
                     WPAALVVVLAVIGWLAWRRSRWTWLAAAAAIPLAVPRIWLPDAAYPATSVQPTSTAAG
                     RAPAARPGTARTSPMPPPDPA"
     gene            complement(2164..>3534)
                     /locus_tag="VHK28_04500"
     CDS             complement(2164..>3534)
                     /locus_tag="VHK28_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141524.1"
                     /translation="DPDYFWHVTAGQWMAENGRVPTTDPFSFTWQGQPWTPHEWLSEL
                     LMFWLVDGLGRTAATVLWGLFPAAIVAVQAAMLARKGVSVRAFVAPAVLIGLVITPYV
                     TLRPQAISWLLLSVLLWFLWELRPEQPRRALLLIPLFVLWANLHGVYVIGLGVVLTYC
                     LFTIAGRTPMSRAKGWMAAGALGAVAASAVTPAGPIGILYPLRYVELSDWGLANIQEW
                     QSPSFHEPAHWAFLALIVAVGVNAGRATPGWLVMLSWVGIALGLVALRNVPIAAVFSL
                     PTLAVGLESRLRARTARKPLPGPMGPSRALGRRVMELGTALIVVAGALVILIPPGVGN
                     GPRANIAERFPVEGVEELRGVDPDVRVLAEYGWAGYVIHELYASGGRVFVDGRNDMYD
                     QQILDDYDAIKAADPGWEQLTDNYGVEALLLAPDVTVTRGPAEASGWCEQYRDEQQVL
                     YLRQCS"
BASE COUNT          487 a         1253 c         1265 g          529 t
ORIGIN      
        1 ggaccacacg ccgcacccca ccctccgcga ccgcgaacgc gagtgcggcg accagcggga
       61 ccgatgcgat cggctccgtc agcccgccgc ccaaggcggt gaggtactgc gccatggcga
      121 tcaccgcgcc agatccggcc agcagcgcga tcccggaggt cacgcccatc cgccgcccga
      181 tccaggccac cagccagcca gtcgccgcag tggcgatcac ggagagcacc caggcaacga
      241 cccacggatc gacgaagggc agcaccgcct gggcggcggc gtacagcagg aagatgcccg
      301 gcggcttgtg atcccaggtc ccgacgtaca gcgcctcccc gttccgcagc tcggacgcca
      361 cgtggacgaa cacggcggga tccagcgacg gtcccacgaa caggcccggc gacaggagca
      421 gcgcggcgag agcgagcaag atcaacgggg gcagcacggg cccgattgtc gccgctgcag
      481 cggaatgggc gttggtactt tgggacgagg cgataccctg catgccgtgc agcgtctcgc
      541 ccaacgcgtg gtcatcggtc ttgcgttcgc gtacgccctg gtgggcgtgt acttgattat
      601 tggcagctgg ggcttcgagg acgtcgatgc ctactgggac gcggccatgc ggctgcgcga
      661 cggcgaaccg ctctacccac ccggccagga cccggacaac taccgggtgt accgatacgc
      721 cccatggttc gcctggctct gggtgccgct gacctacctg ccgcgagcgg ccgttgagat
      781 cggctggggt ctgctggtgg gagtcgccgg agccgctgtc ctgggcgggc tgtttcggct
      841 gcggacggcg gcggccgcgg cgctcgccct gctgctggcg ccgtggatgc tgtccctggt
      901 gcaggtcggg aacatccagc cgctggtggt agccatgctg gggttcggca tctcgcgtcg
      961 aagcgggccg ctgtgggtgg ggatcgccgc ctcgttgaag gcggtgcccg cgctgtgggc
     1021 cctcgtctac gtcgcgcggg gcgagtggcc gcggctcgtc gttgccggtg ccgtcggggc
     1081 tgggctaact gcgaccttcc tcctctacga ccgaacgggt tacgtgaccg atcccggcag
     1141 gtccggcatg agcctgcatt actacttcgg gccggtggcc tggctgattg gcgcggtcgc
     1201 gtcggcgggc gtggccgtgg tgctggcctg gcggcgctcg gactggctct ggccggccgt
     1261 cgcggtggcg gccatgctgg tcgctccccg ctcgcacatc acctacgcca ccttcctggc
     1321 cattgggctg ctcaacggcg cgcgggaccg gatcgatgcg cgctgaggcc gtcgcgcggc
     1381 tggcggcggt ggtcatcgcc gggttcggtg cggtgatcct catcggtgcc ggatggattc
     1441 cgggcatcaa gcccgatgca gacgcctact ggctggccgc catccgcctg cgcgagggcg
     1501 aaccgctcta cctgccggtg agcgcctacg ccaacgagat cgacatctac cgctacgcgc
     1561 cgtggttcgc gtacgcctgg gtgccgctga cgtacctccc gcaggagagc gcctacctgg
     1621 tgtggcgcgc gctgctgtta ggcggggccg tggcggcggt ggtgcccatc ctccggcggc
     1681 tgacgcccgc cgggctggtg ctggccctgc tgatcctcag cctgctggtc agcaacctgc
     1741 cggccgccaa cgtaaccgcg ttgatggtcg gtgccctggc ggtgtcgctg cggacgcgtg
     1801 ccggaccggt cgtgctgggg ctggccgggt cgctgaagat cttccctttg ctgctcgtgg
     1861 ccgggtacgt ggccgagcgt cgctggcgag atgtggcgat cgccctgggc gtggcggcgg
     1921 tgctgtgggc gcacctgctg gctttcgggg tcgaggacta cccgaccgag ttcggcggcg
     1981 tcggactcta ccgggcggcg gcctggctgt ggccggcggc gctggtggtg gtgctggccg
     2041 tcatcggctg gctggcctgg cgtcggtccc gctggacctg gctggcggcc gcggcggcca
     2101 tcccgctggc cgtcccccgc atctggctcc cggacgccgc ctacccggcg acctcggtgc
     2161 agcctacgag cactgccgca ggtagagcac ctgctgctcg tcccggtact gctcgcacca
     2221 gcccgatgcc tccgccggac cccgcgtgac cgtcacgtcc ggcgccagca gcagggcctc
     2281 cacgccgtag ttgtcggtca gctgctccca gccggggtcg gcggccttga tggcgtcgta
     2341 gtcgtccagg atctgctggt cgtacatgtc gttgcgcccg tccacgaaca cccgcccgcc
     2401 ggacgcgtac agctcgtgga tcacgtagcc cgcccagccg tactcggcca gcacccgcac
     2461 gtccgggtcg accccgcgca gctcctccac cccttccacc gggaagcgct ccgcaatgtt
     2521 cgcccgaggc ccgttcccca cgcccggcgg gatcaggatc accagggcgc cggcgaccac
     2581 gatcaacgcc gttcccagct ccatcacccg gcgtcccagc gcccgggagg gccccatcgg
     2641 tccaggaaga ggcttgcgtg cggtgcgcgc gcgcagccgg gattccaggc ccaccgccag
     2701 cgtcggcagg ctgaagaccg ccgcgatcgg cacgttgcgc agggcgacca ggcccagcgc
     2761 gatccccacc caggacagca tcaccagcca gcccggcgtg gcgcggccgg cgttcacccc
     2821 aactgccacg atcagcgcca ggaaggccca atgcgccggc tcgtggaagg acggcgactg
     2881 ccactcctgg atgttggcca gcccccagtc ggagagctcc acgtagcgca gcgggtacag
     2941 gatgccgatg ggtcccgccg gcgtgaccgc cgacgccgcc accgccccca gtgcgcccgc
     3001 cgccatccag cccttggcgc ggctcatggg cgtgcgcccg gcgatggtga agaggcagta
     3061 cgtgagcacg acgcccagcc cgatcacgta caccccgtgc aggttggccc acagcacgaa
     3121 gagggggatc agcaggaggg ctcgtcgggg ctgctcgggg cgcagctccc agaggaacca
     3181 gagcaggacg ctgagcagca gccaggagat tgcctggggt cgcagggtca cgtatggcgt
     3241 gatcaccaga ccgatgagga cggccggcgc cacgaatgcc cggaccgata cgccctttcg
     3301 ggcgagcatg gcggcttgga ccgcaacgat ggccgcgggg aagaggcccc agagcacggt
     3361 ggcggcggtg cggcccaggc cgtccaccaa ccagaacatg agcagctcgg agagccactc
     3421 gtgcggcgtc catggctggc cctgccaggt gaagctgaac gggtcggtgg tcgggacgcg
     3481 gccgttctcg gccatccact ggccggccgt cacgtgccag aagtagtccg gatc
//
LOCUS       DATACA010000217         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2197072, whole genome shotgun sequence.
ACCESSION   DATACA010000217 DATACA010000000
VERSION     DATACA010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197072"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VHK28_04505"
     CDS             <1..1231
                     /locus_tag="VHK28_04505"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX2141525.1"
                     /translation="LAWTALQPGRTADALFMLNAATDFDEFREAASRFEVPAQNIVYA
                     DVDGHIGYQAPGKVPVRGAGDGDWPAPGWDPDYDWQGYVPFEDLPWVLDPPSGYVVAA
                     NNAVIDDNFGIYLTDDLDRGFRAARIVELLEAAGPLDPGGMLEIDFDQRGPLAHVLVP
                     ALDELRSNPATEADVRDALALLDGWGGMQTPDSVPAALVNAVWRNLLAWTFHDELSPL
                     GEEMLPDGGDRWMEVVLGLLDDPDDVWWDDVTTPEQREDRDTILAASARDAVDNLRSA
                     LGEDPDEWRWGRMHRLELRNATFGESGVGLIEGLFNRGWWELGGGADVVDATGWTAHF
                     GYEVDWVPSMRMVVDLSDLDASRYINLTGVSGHAFHHHYDDQTDLWARGETIPWPFSI
                     AAVEDAAEDVLVLEPGG"
     gene            complement(1251..>2210)
                     /locus_tag="VHK28_04510"
     CDS             complement(1251..>2210)
                     /locus_tag="VHK28_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX2141526.1"
                     /translation="GLWCRLDGFQTDDLASLLVNRRVARVMGLLRGTIHLVTARDALS
                     IRAMSAPVMQRIWRSTSFAKALEGLETDAVVAHGRHLLAERPQTAAELGRRLQERWPD
                     RDAQSLAYAVTFHVPLVQPPPRGLWGRSGRAMPTLLEDWLGAGPDPQPSVDELVIRYL
                     RALGPAAPQDAATWSRFTALREVFDRLRPRLRTFRDENGRVLFDVPDGPLPNPDTPAP
                     VRFLPEYDNVVLSHHDRSRIIDRRWPQATWMRGTILVDGFVRGTWRLDREREVAVLSV
                     AILDEIRPEQRDEVEHEADALLAFAASDAKEHRVREVGAGTTP"
BASE COUNT          331 a          765 c          795 g          319 t
ORIGIN      
        1 cctggcgtgg acggcgctgc agccgggccg cacggcggac gccctcttca tgctgaacgc
       61 ggcgaccgat ttcgacgagt tccgcgaggc ggccagccgc ttcgaggtgc cggcgcagaa
      121 catcgtgtac gccgacgtcg acgggcacat cggctaccag gccccaggga aggtcccggt
      181 gcgtggcgcg ggtgacggag actggccggc gccaggctgg gaccccgact acgactggca
      241 ggggtacgtt cccttcgagg acctgccgtg ggtcctggat cccccgtcgg gctacgtggt
      301 ggcggccaac aacgcggtta tcgacgacaa cttcggcatc tacctgaccg atgacttgga
      361 ccgcggcttc cgcgccgccc gcatcgtcga gctgctggag gcggccggtc ccctggatcc
      421 tggcggcatg ctggagatcg acttcgacca gcgcggcccg ctggcccacg tcctggtgcc
      481 cgccctggac gagctgcggt cgaaccccgc gacggaggcc gacgtgcggg acgcgctggc
      541 cctgctggat ggctggggcg ggatgcagac gcccgactcg gtgcccgccg ccctggtcaa
      601 cgcggtgtgg cgcaacctgt tggcctggac cttccacgac gagctgtcgc cactcgggga
      661 ggagatgctg ccggacggtg gggaccggtg gatggaggtc gtgcttgggc tgctggacga
      721 ccctgacgac gtgtggtggg acgacgtcac gacgccggag cagcgcgagg accgtgacac
      781 gatcctggcc gcctcggcgc gcgacgcggt ggacaacctg cgatcggccc tgggcgagga
      841 tccggacgag tggcggtggg gtcgaatgca ccggctggag ctgcgcaacg ccaccttcgg
      901 cgagtcgggc gtcggcctga tcgaggggct cttcaaccgc ggctggtggg agctcggcgg
      961 cggggcggac gtggtggatg ccaccggctg gacggcacac ttcggctacg aggtggattg
     1021 ggttccgtcc atgcgaatgg tcgtcgacct gtcggacctg gacgccagcc gctacatcaa
     1081 cctcacaggt gtgagcggcc acgccttcca ccaccactac gacgaccaga cggacctgtg
     1141 ggcgcgcggc gagaccatac cctggccgtt cagcatcgcg gccgtggagg acgccgccga
     1201 ggacgtgctg gtgctcgagc ccggcggcta acgtcgcgcg cgcgtagtcc tcacggtgtc
     1261 gtgcctgctc cgacctcccg cacccggtgt tcctttgcgt ccgacgcggc gaaggcgagc
     1321 agcgcatctg cctcatgctc gacctcgtcc cgctgctccg gtctgatctc gtccaggatg
     1381 gccaccgaca gcacggcgac ctcccgctcc cgatccaggc gccaggtgcc gcgcacgaag
     1441 ccatcgacca ggatcgtgcc gcgcatccag gtggcctgcg gccagcgccg atcgatgatc
     1501 cggctgcggt cgtgatgcga cagcaccacg ttgtcgtact cgggaaggaa gcgcaccggc
     1561 gccggcgtat ccggattggg cagcggcccg tccgggacgt cgaagagcac acgaccgttc
     1621 tcgtcgcgga acgtccgcag ccgcggccgt agccgatcga acacctcgcg cagggcggtg
     1681 aagcgcgacc aggtggctgc atcctgcggg gccgccggac ccagggcgcg caggtacctg
     1741 atcaccagct cgtcgaccga tggctgcggg tccggcccgg cacccagcca gtcctcgagc
     1801 agggtcggca tggcccggcc ggagcgaccc cacaacccgc gcggcggggg ctgtaccagc
     1861 gggacgtgga aggtgacggc gtacgccagc gactgcgcgt ctcggtccgg ccagcgttcc
     1921 tgcaagcgcc gtcccagttc tgccgcggtc tgcggccgct ccgcgaggag atggcggccg
     1981 tgcgccacga cggcatcggt ctctaacccc tcgagcgcct tggcaaacga tgtcgaccgc
     2041 cagatccgct gcatgaccgg agcggacatg gcacggatcg acagcgcgtc gcgcgcggtg
     2101 accaggtgga tggtcccgcg cagcaggccc atcacgcgcg ccacgcgtcg gtttaccagg
     2161 agcgatgcca ggtcatcggt ctggaaaccg tccagccgac accacaggcc
//
LOCUS       DATACA010000218         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_28697, whole genome shotgun sequence.
ACCESSION   DATACA010000218 DATACA010000000
VERSION     DATACA010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28697"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1118)
                     /locus_tag="VHK28_04515"
     CDS             complement(330..1118)
                     /locus_tag="VHK28_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2141527.1"
                     /translation="MATQTADKAQDKAQAQEENVEETQERRRGGDRRAAGQKARIVIV
                     DGHTLFRRGVRNILDLEPDMEVVGEAGSGREALAVVEELTPDVVLMDLSLPAPNGIET
                     TQRIKRELPHTGVVVLAPNDDEDQLFDAIKAGAAAYVLKDIDPGDLIAIIRRVRSGEY
                     LINDKVFSKPAVASRVLKEFRELAVYGSDAQPIFAPLSPREVEILDNIAQGMTNKQVA
                     YALSISEQTVKNHMSSILRKLSVNDRTQAVVYAMRQGWIKIPED"
     gene            complement(1142..2134)
                     /locus_tag="VHK28_04520"
     CDS             complement(1142..2134)
                     /locus_tag="VHK28_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2141528.1"
                     /translation="MTAITFDRIQAQATDSVRRDARRVRELADRFRSAYRERLEDWVR
                     MRASLETQHKPATRAATTAADRLRTRHEQDGRELASLQGRLKRLDVAARQLELVTSYL
                     ASPDPAVDEPALKDTDQSHAAMPLRIIQAQEGERQRLAEVVHDGPAQVLTNAIFQVEF
                     LDRVIDDSPRTAHAELAFLRTMLRDGLDEVRGFIAELRPPAVDVGLSQALTDAGAAFA
                     SRHGIVVEVAVDGIDESLTPDEKASVLRIVQEALQNVRKHSAASQVRISLEDGCLVIG
                     DNGRGFDVMRLASGASRNFGLHFMRERAELMGSTLQIESRQGEGTRLLLRLPRR"
BASE COUNT          399 a         1002 c          946 g          498 t
ORIGIN      
        1 tcggtccatg gggacaggcc ggggtggtgc tgggtgccgc cgtcaccggc tacgggctgt
       61 gggcaatgaa gctggccgac ttcgtcgacc gggggctgcc gttcgccgcc tgatcgccgg
      121 acaaaatgag ggagccgtgc ctgctgcgac acggctcccg caccggagga ggagacccga
      181 ggcagttacc cgctcgcgat aaccggggaa cgcctcgttc agatgaaggc cgttgcggat
      241 acccgccccg acttggctcg aatctagaga acggggatct gccgggccat ggcacgaccg
      301 taccggcccg gggggtgacc tccggcggct cagtcctcgg ggatcttgat ccagccctgg
      361 cgcatggcgt acaccacggc ctgcgtccgg tcgttgaccg acagcttgcg gaggatggag
      421 ctcatgtggt tcttcaccgt ctgctcgctg atcgacagcg cgtaggcgac ctgcttgttg
      481 gtcatgccct gcgcgatgtt gtccaggatc tccacctccc gcggcgagag cggcgcgaag
      541 atcggctggg cgtccgaccc gtagaccgcc agctcgcgga actccttgag gacgcgcgag
      601 gcgaccgccg gcttgctgaa gaccttgtcg ttgatcaggt actcgcccga ccgcacgcgg
      661 cggatgatcg cgattaggtc gccgggatcg atgtccttga ggacgtacgc cgcggcgccg
      721 gccttgatgg cgtcgaacag ctggtcctcg tcgtcgttgg gggccaggac caccacgccg
      781 gtgtgcggca gctcgcgctt gatgcgctgc gtggtctcga tcccgttggg ggcgggcagc
      841 gagaggtcca tgagcacgac gtccggcgtc agctcctcca ccaccgccag cgcctcgcgg
      901 ccgctgccgg cctcgcccac gacttccatg tccggctcga ggtcgaggat gttgcgcacg
      961 cctcgacgga acagggtgtg cccgtccacg atcacgatgc gcgccttctg cccggcggcg
     1021 cgtcggtcgc cgcctcggcg acgttcctgg gtctcctcca cgttctcctc ctgtgcctgg
     1081 gccttgtcct gggccttgtc tgcggtctgg gtggccatgg tcttcactct cctcgttacg
     1141 gtcagcgcct gggaagacgc agcagcagtc gcgtaccttc tccctgtcgc gactcgatct
     1201 ggagcgtgga gcccatcagc tctgcccgct cccgcatgaa atgcagcccg aagttccggc
     1261 tcgcccccga ggccagccgc atcacgtcga agccgcgtcc gttgtcgccg atgaccaggc
     1321 acccatcctc caatgagatg cgcacctggc tggcagctga gtgcttgcgc acgttctgga
     1381 gcgcctcctg cacgatgcga aggaccgatg ccttctcgtc gggcgtcagg ctctcgtcaa
     1441 tgccgtccac cgccacctcg accacgatgc cgtgccggct ggcgaaggcg gcgcccgcgt
     1501 cggtcaacgc ctgggacagc cccacgtcca cggccggtgg ccgcagctcg gcgatgaacc
     1561 cacgcacctc gtccaaccca tcgcggagca tggtgcgcag gaatgccagc tcggcatgcg
     1621 ccgtccgcgg cgagtcgtcg atgacgcggt ccaggaactc gacctggaag atggcgttgg
     1681 tcagcacctg cgccgggccg tcgtggacga cctcggccag gcgctggcgt tccccctcct
     1741 gggcctggat gatgcgcagc ggcatggccg cgtgcgactg gtcggtgtcc ttcagcgccg
     1801 gctcgtcgac ggccgggtcc gggctggcca ggtagctggt caccagctcc aactggcgag
     1861 cggcgacgtc gagccgcttg agccgtccct gcaggctggc cagctcccga ccgtcctgct
     1921 cgtgcctggt gcgcagccga tcggctgccg ttgtggcggc acgagtggcc ggcttgtgct
     1981 gcgtctcgag gctggcccgc atccgtaccc agtcctccaa ccgctcgcgg taggcggagc
     2041 ggaagcggtc cgccagttcg cggacccgcc gcgcgtcgcg gcggacgctg tctgtcgcct
     2101 gtgcctggat gcggtcgaag gtgatggccg tcaccgcggc cgtggctgtt ttccccaagg
     2161 ttccttccga tgcgtgccct gcgagtacca gcccgcgcgt agtaccggac cggacgccca
     2221 tcctgtcggg gtgagttgct cacggtcaac agtactaacc gcgccggaag tactacccgc
     2281 ctttcctgcg ggctgtcgtg cgcccgaatg cggtacccac gcggccgcat ttccacgatg
     2341 tgtctggctg ccggacgctt cgtggggaaa tgcagcacgg gcgcggcgta acgaccacga
     2401 tctggccggt cctgccgcac ttcgtcgctg ttgcgcctca ccggcacggc aattcccgaa
     2461 gaaggcacgc ctccgacgcc aaatcgtgga caagctgcgt tacgagtacg gcctgcccgg
     2521 cgtatgcggc agggatgcac gcgagccggc cgccacgtgc tgtcctgccg atacagcggg
     2581 catgcccgag cgtgacgcga caagaacttc gcccctcccg cgtgcggctg cccgctacat
     2641 cagcgttctg acccccggct cgccccccac gtgaagatca gcctgaaatg tcggcagccg
     2701 cgcacccgac ggcggctagc atccactcaa tgaccggtca gcggcatctc gtggcgaccg
     2761 ccacagcgtg ggccgccctg gccatgggct ggttcgtggc agcgttctca ctgcccatcg
     2821 acgacttctg gctctccctg gcctc
//
LOCUS       DATACA010000219         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1956530, whole genome shotgun sequence.
ACCESSION   DATACA010000219 DATACA010000000
VERSION     DATACA010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956530"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VHK28_04525"
     CDS             complement(<1..551)
                     /locus_tag="VHK28_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2141529.1"
                     /translation="MDQHATDLLTPDAEAADRERWLARRDRVPSVWARYTDLVVERGE
                     GSWIETVDGERYLDYTSGIGVTNTGHAHPRVAAAIAHQAARIIHAQQNILYHKAGLDL
                     HERLPRTMPGLADGEEAALFLSNSGAEAVEAAVKLAKYATRRPAVIAFRGGFHGRTHG
                     TMALTSSGVKMRGHYEPLLGSVH"
     gene            complement(524..1372)
                     /locus_tag="VHK28_04530"
     CDS             complement(524..1372)
                     /locus_tag="VHK28_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2141530.1"
                     /translation="MTSTGQAAHAAAVHPSREREGPTYRIGEAARMVDTSPSALRSWE
                     RQGLVRPRRTRSGYRLYTDSDLAVLRRVRHMRSERVSAPGIRRLIAATPGRTSDGEAR
                     RRRRLDGRRVRSLRLEQGLSLREAAARTGLSVSFLSAVERDASGVSVASLQRLTAAYG
                     VTLLDLFSPATVGQLVRPAQRRVLELQGSGVRIEQLASTSVHLEPQLFVLQPGSSSDG
                     EYAHGGEEFLFVLDGAVTLWVGDHDCYRLRTGDALTFPSTLPHRWRNRAGGETRLLWI
                     NTPPTF"
     gene            complement(1369..>1574)
                     /locus_tag="VHK28_04535"
     CDS             complement(1369..>1574)
                     /locus_tag="VHK28_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141531.1"
                     /translation="DYVITSFVAGQATTFPLWVFGVSRLGVPPEVNVLGTLIFGIALL
                     FIAIQVLTQRRRATAEAVAARVR"
BASE COUNT          215 a          549 c          590 g          220 t
ORIGIN      
        1 aagtggacgc tgccgagcag cggctcgtag tggccgcgca tcttcacgcc gctgctggtg
       61 agggccatgg tgccgtgggt gcgtccgtgg aagccgccgc ggaaggcgat caccgccggc
      121 cgccgggtgg cgtactttgc cagcttgacg gccgcctcca ccgcctcggc ccccgagttg
      181 gagaggaaga gcgccgcctc ctcgccatcg gccaggccgg gcatggtgcg cggcagccgc
      241 tcgtgcaggt cgagcccggc cttgtgatac aggatgttct gctgcgcgtg gatgatgcgg
      301 gccgcctgat gggcgatggc ggccgccacc cgcggatggg cgtgcccggt gttggtcacg
      361 ccgatgccgc tggtgtagtc caggtagcgc tccccgtcga ccgtctcgat ccaggatccc
      421 tcgccgcgct ccacgaccag gtcggtgtag cgggcccaga ccgacggcac ccggtcgcgc
      481 cgcgccagcc agcgctcacg gtcggcagct tcggcatcgg gggtcagaag gtcggtggcg
      541 tgttgatcca tagcagtctg gtctccccgc cggcgcggtt gcgccagcga tgcggaaggg
      601 tggacgggaa ggtgagcgcg tcgccggtac gcagacggta gcagtcatga tcgccgaccc
      661 atagggtgac ggcgccgtcc aggacgaaca ggaactcctc gccgccgtgc gcgtactcgc
      721 cgtcgctgct ggatccgggc tgcagcacga acagctgggg ctccaggtgg accgatgtcg
      781 acgccagctg ctcgatccgc accccggagc cctggagctc caggacgcgc cgctgggcgg
      841 gtcgcaccag ctgcccgacc gtcgccgggc tgaagaggtc cagcagcgtg accccgtatg
      901 cagctgtcag gcgctggagg ctggccacgc tgacgcccga ggcgtcccgc tcgactgccg
      961 acaggaagct gaccgacagg ccggtgcgtg ccgcggcctc gcgtagcgag agcccctgtt
     1021 ccaggcgcag gcttcgcacg cggcgtccgt ccagccggcg ccggcggcgc gcctcgccat
     1081 cgctcgtgcg gccgggcgtg gccgcgatca ggcgccggat gccgggcgcg ctgacccgct
     1141 cgctgcgcat gtggcgcaca cggcgcagca cggccaggtc cgagtcggtg tagagccggt
     1201 agccgctgcg cgttcggcgc gggcgcacca gtccctgccg ctcccacgac cggagggcgg
     1261 agggcgaggt gtccaccatg cgcgccgcct ccccgattcg gtaggtgggg ccctcacgct
     1321 cccggctggg atggacggcg gcggcgtgcg ccgcctggcc tgtggaagtc atcgcacccg
     1381 ggccgcgacg gcctcggccg tggctcgccg ccgctgggtc aggacctgga tggcgatgaa
     1441 gagcaacgcg atcccgaaga tcagcgtgcc gagcacgttg acctccggcg gcacgcccag
     1501 gcggctgacg ccgaagaccc acagcgggaa ggtggtggcc tgaccggcca cgaaggaggt
     1561 gatcacgtag tcgt
//
LOCUS       DATACA010000220         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_350362, whole genome shotgun sequence.
ACCESSION   DATACA010000220 DATACA010000000
VERSION     DATACA010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350362"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VHK28_04540"
     CDS             <1..686
                     /locus_tag="VHK28_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007469029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141532.1"
                     /translation="VDTRRARVGLLVAGNTYRHPAVLANMAAALDVITGGRLEFGIGA
                     GWHEGEHRMYGIPLGTAGQRIRALDEACEVITSLWTREVSTFSGQYYALAEARCEPKP
                     LQRPRPPITIGGTGEKLTLRVVAKHADRWNFNGKSVDEFVRLNGILDAHCSAVGRDPS
                     TIIRSVQRHLSEDLESAVPQVAAYIAAGADHVVLNPRIPLRPGIAEAVTRRVIEPVLN
                     EVDRGGANQ"
     gene            757..2166
                     /gene="tcuA"
                     /locus_tag="VHK28_04545"
     CDS             757..2166
                     /gene="tcuA"
                     /locus_tag="VHK28_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX2141533.1"
                     /translation="MPANRLAPSETRRRVVVIGGGNAALCAAMAAHDAGASVTVLERA
                     EKPFRGGNTRHTRNIRTVHSGADGYVTGPYLYDEFLADLASVTGSDADPALAAQTIAE
                     SETITAWMMEHGANWQAPLKGTLGLARTNHFFLGGGKSLLNAYYIRAQDAGIEIHYEA
                     MVTGLDLDGRHCRGVRVRFGTDSEEEYVQADAFVVASGGFEANRDWLAEYWGPAAYNF
                     IVRGTANNDGSVLRELLDAGARRVGDPGAAHAIAVDARAPKYDAGIITRLDAVPIGIA
                     VNCEAKRFYDEGEDMWPKRYATWGRLIASQPEQLAFAIFDNKVANETIPGLYPPYRAE
                     TIEELATQLGLDPVAVGRTVDEFNASCQVGRFDLSELDDCRTEGLVPPKSHWARPIDT
                     APYYGYPLRTGITFTYMGVAVDPEARVQCDGDGAFENVFAAGEIMSGNVLTRGYLAGF
                     GMTIGSVWGRIAGGSAARA"
     gene            2159..3259
                     /gene="tcuB"
                     /locus_tag="VHK28_04550"
     CDS             2159..3259
                     /gene="tcuB"
                     /locus_tag="VHK28_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEX2141534.1"
                     /translation="MLELPVVEEARRQLDICNACRYCEGICAVFPALERRSFFDVGDM
                     TYLANLCHDCRACLDVCPYAPPHELGVDIPRLMSEVRERTYSHYAWPQALSSRVDRSV
                     GLALGFSSVAVVAVVVATFMANGWDDMFTASSAVGSFYEVIPWLAMMLPFLAVSIMAI
                     GILAVAGVRFWRDTGRGWPFGVSTLAQATLDVALLRNLDGGGPGCTYPQERPNQRRRI
                     YHSLVFYGFISAFISTTLAALYQDFLGIFPPYDYLSLPVIFGTVGGVAMIAGILGLML
                     DKRKSDKRRIATRMRSMDIAFIFALLLVNVTGIAVLALRETAAMPTLLAVHLGYTAAL
                     FVTLPYGKFAHAVYRYLALIRNRLELVEERLG"
     gene            3392..>4398
                     /locus_tag="VHK28_04555"
     CDS             3392..>4398
                     /locus_tag="VHK28_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141535.1"
                     /translation="MRRILVLAAVAALLVAALPSAVSAQPPTRFSEQRLNLSCEANSP
                     ELGYVTLGLGVSQDFTGAGLAYWAPGSVPFESDPVLVSDESEVIGSVESTQLDVTLQM
                     YAFDPEVLYGEPAGEAVATLTLTPDGDPESFTLQSDNTNARFRSEEVHQPLAVGGSVS
                     LPDGTTFDLSECMAVRSEITVFATNPNAFVSRYAATTVSCEWFEELSSISLWAVADSG
                     GSMTQLFVADSSGFYFASGTLELHGRGLAAHYELFPGEGEPSDLPAGSATASATLTAS
                     GDREQFVDRFGNETIKRVSQRLMVDGSLALTTPAGTRVLPMDDASCSASADRELYRVI
                     MP"
BASE COUNT          720 a         1481 c         1475 g          722 t
ORIGIN      
        1 gcgtggacac ccgccgggct cgagttggcc tgctggtggc aggcaacacc taccggcatc
       61 ccgccgtgct ggccaatatg gccgcggcgc tggatgtaat taccggcggc cggctcgagt
      121 tcggaatcgg cgcggggtgg catgaaggcg agcaccgcat gtacggcatc ccgctgggca
      181 cggccgggca gcgcattcgt gcgctggatg aggcatgcga agtcatcacc tccctctgga
      241 cgcgggaggt gtcgacgttc agcgggcagt actacgcact ggccgaggcc cgctgcgagc
      301 cgaagccact tcagcgcccg cgtccgccga tcaccattgg cgggaccggc gagaagctga
      361 cgctgagggt cgtcgcgaag catgccgacc gctggaactt caatggaaag agcgtcgacg
      421 agttcgtacg cctgaacggc atcctcgacg cccactgctc ggcggtcggg cgcgacccgt
      481 cgaccatcat tcgatccgtg cagcgccatc tcagcgagga cctggagtcg gcggtcccgc
      541 aggtcgcagc ctacatcgcg gcaggagccg accacgtggt gctcaacccg cgcattccgt
      601 tgcgacccgg aatcgcggag gccgtcaccc ggcgggtgat cgagccggtc ctgaacgagg
      661 tggaccgggg cggcgccaac caataggaca ccgccgccgc ggcccggttc ttggtaaaca
      721 atcgtatacg cgcagagagc acgagggtta ttgaggatgc cggcaaaccg cttggcgcca
      781 tcagagaccc gacggagggt ggtcgtgatc ggcggtggca acgccgcgct ctgtgctgcc
      841 atggctgccc acgatgcggg cgcatcggtc accgtgctcg agcgtgccga gaagccgttt
      901 cgaggcggga atacccgcca cactcgcaat attcgcacgg tccactccgg ggccgacggc
      961 tacgtgaccg ggccgtacct gtacgacgag ttcctggcgg acctggccag cgtgaccggc
     1021 tcggatgccg atcccgcgct ggcagcacag accatcgccg agtcggagac catcaccgcc
     1081 tggatgatgg agcatggggc caactggcag gcaccgctca agggaacgct cgggctggca
     1141 cgcaccaatc acttcttcct gggcggcggc aagtccctgc tgaacgccta ctacatccgg
     1201 gcccaggacg ccggcatcga gattcactac gaagccatgg tcaccgggct ggacctggac
     1261 ggccgtcatt gccgcggggt tcgggtccga ttcggaactg acagcgaaga ggagtacgtc
     1321 caggccgatg ccttcgtggt cgcgtcgggg ggcttcgagg ccaaccgcga ctggttggcc
     1381 gagtactggg gaccggcggc ctacaacttt atcgtgcgcg gcacggcgaa caatgacggc
     1441 tcggtgctgc gcgagctcct ggatgccgga gcacgccgcg ttggtgatcc aggcgcagcg
     1501 cacgccatcg ctgtcgacgc ccgggcaccg aagtacgacg ccggcatcat cacccggctg
     1561 gatgcggtgc cgatcggcat cgccgtcaac tgcgaggcca agcgcttcta cgacgagggc
     1621 gaggacatgt ggccgaagcg gtatgcgacg tggggccgcc tgatcgcgtc ccagccggag
     1681 cagctcgcct tcgccatctt cgacaacaag gtcgccaacg agaccatccc cggcctctac
     1741 ccgccgtatc gcgccgagac catcgaggag ctggccaccc agctgggcct cgacccggta
     1801 gccgtgggac ggaccgtgga cgagttcaac gccagctgcc aggttggtcg cttcgacctc
     1861 tccgagctcg atgactgccg caccgagggg ctggtgccgc cgaagagcca ctgggcccgc
     1921 cccatcgaca ccgcgccgta ctacggctat ccgctgcgta ccgggatcac gttcacctac
     1981 atgggcgtgg cggtcgatcc cgaagcgcgg gtgcagtgcg atggcgatgg ggccttcgag
     2041 aacgtcttcg cggccggcga gatcatgagc ggcaacgtcc tgacacgcgg ctacctggcc
     2101 ggcttcggca tgaccatcgg cagcgtctgg ggccggatcg ccggcgggag cgccgcccgt
     2161 gcttgagctg ccggtggtcg aggaggccag acgccagctc gacatctgca acgcgtgccg
     2221 ctactgcgag ggcatctgcg ccgtcttccc ggcgctcgag cgtcgaagct tcttcgacgt
     2281 cggcgacatg acctatctgg ccaacctttg ccacgactgt cgcgcatgcc tggacgtctg
     2341 cccatacgcg ccaccgcacg agctgggggt ggacatcccg cggctgatga gcgaggtgcg
     2401 cgaacgcact tactcccact acgcctggcc ccaggcgctc tccagccgcg tggaccgcag
     2461 cgtggggctg gcactggggt tctcatcggt ggctgtggtc gcggtggtgg tggccacctt
     2521 catggccaac ggatgggacg acatgttcac cgccagcagt gcggtgggtt ccttctacga
     2581 ggtgatcccc tggctggcca tgatgctgcc gttcttggcc gtttcgatca tggccatcgg
     2641 catcctggcg gttgccggtg tgcggttctg gcgtgatacg gggcgtggct ggccgttcgg
     2701 cgtgagcacg ctggcgcagg cgaccctgga cgtggcgctg ctgcgcaacc tggacggagg
     2761 tggaccgggc tgcacctacc cgcaggagcg gccgaaccag cgacgccgga tctaccactc
     2821 cctggtcttc tacggcttca tctcagcgtt catctcgacg acgctcgccg ccctctacca
     2881 ggacttcctg ggcatctttc ccccgtatga ctacctcagc ctgccggtca tcttcggcac
     2941 ggtgggcggg gtggccatga tcgcgggcat cctgggacta atgctggaca agcggaagtc
     3001 ggacaagcga cgcatcgcaa cgcgcatgcg cagcatggac atcgccttca tcttcgccct
     3061 gctgctggtc aacgtgaccg gcatcgctgt actcgcgctg cgagaaacgg cggccatgcc
     3121 gaccctgctg gccgtccact tgggctacac ggcggcgctg ttcgtgacgc ttccctacgg
     3181 caagttcgcc cacgcggtat accgatatct cgccctcatc cgcaaccggc tcgagctcgt
     3241 ggaggagcgg ctcggctgac ccgagtctcg ccgcagttcg gccgatgccg taccgcagcg
     3301 acccgaggtg agagtgctcc caaccacggg agcgggcacg cgaacgcatc gggtctcggc
     3361 cctcccgtac acagcctggg agggcactca catgcgacgc atcctcgtcc tcgccgcggt
     3421 ggcggcattg ctggtggcag cgttgccatc ggccgtcagc gctcagccac ccacgcgatt
     3481 ctcagagcag cggcttaacc tgagctgcga agccaactca ccggagctcg ggtacgtgac
     3541 cctggggctc ggcgtgagcc aggacttcac cggtgccggg ctggcgtact gggcgccggg
     3601 cagcgtgccg ttcgaaagcg acccggtgct ggtatcggac gaaagcgagg tcatcggctc
     3661 ggtggagagc acccagctcg acgtcaccct gcaaatgtac gcgttcgatc cggaggtgct
     3721 ctatggcgag ccggccggtg aggcggtggc aaccctcacc ctcacgcccg atggcgaccc
     3781 ggagtccttc acgctgcaga gcgacaacac caacgcccgg ttccggtctg aggaagtgca
     3841 ccaaccactg gccgtcggcg gcagcgtgag cctgccggat gggaccacct tcgacctgtc
     3901 cgagtgcatg gcggtccgca gcgagatcac cgtgttcgcc accaatccca acgcgttcgt
     3961 ctccaggtac gccgcgacga ccgtcagctg cgagtggttc gaggagctca gcagcatcag
     4021 cctgtgggca gtcgccgatt ccggcggcag catgacccag ctcttcgtcg ccgactcgag
     4081 tgggttctac ttcgcttccg gaaccctgga gctgcacggt cgcggcctgg cggcccatta
     4141 cgagctcttc cccggtgagg gcgagccgag cgacctgccg gcgggcagcg ccacggcgtc
     4201 ggcaaccctg accgcgtccg gagatcgaga gcagttcgtt gaccgcttcg ggaacgagac
     4261 gatcaagcgc gtcagtcagc ggctgatggt cgacggctcg ctcgccctga ccaccccggc
     4321 tggcacgcgg gtgctgccca tggatgatgc gagctgctcg gcgtctgccg accgcgagct
     4381 ctatcgggtc atcatgcc
//
LOCUS       DATACA010000221         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1715715, whole genome shotgun sequence.
ACCESSION   DATACA010000221 DATACA010000000
VERSION     DATACA010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715715"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VHK28_04560"
     CDS             complement(<1..447)
                     /locus_tag="VHK28_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141536.1"
                     /translation="MTHDSSAAWSPRTYLLRAAGLPRTVTPPMDRAALDWYNRFPEGW
                     WGYHWTLPRPMSAVEIVASGSLDARLMATLWAVVARRRGVMLASEAPQAGKTTTLSAL
                     VDFLPDDTVGVFLRGWAQDMSWTDEIGPDRGYLLVNEMSDHLPIYVW"
     gene            690..1211
                     /locus_tag="VHK28_04565"
     CDS             690..1211
                     /locus_tag="VHK28_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2141537.1"
                     /translation="MAVQLMAGGEQLAAIARRDPEAFTALVAEHDRALVRLCYVITGD
                     REQARDAAQSTWQRLWRDPPRMRDASKLRSWLLRVAANEARQVARRRRRGLELERENA
                     ADSPQRPDHDELLDLAATLGRLGHQDRELLALRYVLELPSPQIAEHLGISAEGVRSRL
                     HRLLGRLRQEMDR"
     gene            1208..1744
                     /locus_tag="VHK28_04570"
     CDS             1208..1744
                     /locus_tag="VHK28_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141538.1"
                     /translation="MTPFELRLSSELRAYVEAADLDRGPEAVVKDVLAAPSRRRGWRL
                     PAGIAAAVLIVAGAATGIRALDGMASRPAEASVRGQAYGLAVVRSLEFAPGDLSPYGE
                     VSSSEEAYFADETAYAVRGVDPSVALVVRLEPGLADDTGPWGEWMLLTRGPAPNSLCP
                     YYDPESSFTPRDCLEHGE"
     gene            1741..2139
                     /locus_tag="VHK28_04575"
     CDS             1741..2139
                     /locus_tag="VHK28_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141539.1"
                     /translation="MSRTRLAAIGAAALAALAGLVAYIDGDHDIAPFFVALALAGGIQ
                     AAIVRQPFRGGRRAVAWAIAGGWLIAAVWIGALLAWWQAMCACSYPPRSPEETYLGLT
                     ATAYHLIGVYGGAALVIASAVTAELRRRAG"
BASE COUNT          318 a          751 c          783 g          321 t
ORIGIN      
        1 ccagacgtag atcggcaggt ggtcgctcat ctcgttgacc aggaggtagc cgcggtccgg
       61 cccgatctca tcggtccagg acatgtcctg cgcccagccg cggaggaaga cgcccaccgt
      121 gtcatcgggc aggaagtcga ccagcgccga gagggtggtg gtcttgccgg cctgcggtgc
      181 ctcgctggcc agcattaccc cgcggcggcg ggcaacgacc gcccacaggg tcgccatcag
      241 ccgggcgtcc aggctgccgg aggcgacgat ctcgaccgcg ctcatcggcc ggggcagcgt
      301 ccagtggtag ccccaccacc cttccgggaa gcggttgtac cagtccagcg ccgcgcggtc
      361 cattggaggc gtcacggtac gcggcagccc cgcggcgcgc aacaggtacg tccgcgggga
      421 ccaggcggcg gacgaatcgt gcgtcaccgg tacggcaggt cgttgacgaa atgcagtggt
      481 ccacgctact tcgtacggaa atgccgcacc cacgcggcct aacagtcacg aaacacgctc
      541 ggcgagccgg gttcgtcggg ataacgcaat gcccgtacga catttcccaa cgaattggcc
      601 gccgcgcccc acccttcgtg gtgaagctgc cacgccggtg cggcatcgca ctcctgaaac
      661 accggcggcg cctcgttcat ctgtagggtg tggcagtgca gctcatggcc ggtggcgagc
      721 agctcgcggc gatcgcgcga cgcgacccag aagcgttcac cgccctggtc gccgagcacg
      781 accgagccct ggtgcggctc tgctacgtca tcaccggcga ccgagagcag gcccgcgacg
      841 ccgcgcagag cacctggcag cggctgtggc gcgatccacc ccgcatgcgg gacgcgtcga
      901 agcttcgctc ctggctgctg cgggtggctg ccaacgaggc ccggcaggtg gcacgtcggc
      961 ggcggcgagg gctggagctg gaacgcgaga acgccgctga cagcccccag cggcccgacc
     1021 acgatgagct cctggacctg gccgccacgc tcggccgcct cgggcaccag gaccgcgagc
     1081 tcctcgcact gcggtacgtg ctggagctgc cgtcgccgca gatcgcggag cacctcggaa
     1141 tcagcgctga aggcgtgcgc tcccggctcc atcgcctact gggacgcctg cgtcaggaga
     1201 tggaccgatg actcccttcg agctgcgcct gtccagcgag ctgcgtgcct acgtggaggc
     1261 cgccgatttg gatcgcggcc ctgaggccgt ggtgaaggac gtactcgccg cgccgtcccg
     1321 caggcggggc tggcgtctcc cggcaggcat agcggccgcc gtgctgatcg tggccggtgc
     1381 cgcgacgggc atccgcgcgc tggacggaat ggcgtccagg ccggcggagg catcggtccg
     1441 gggccaggcc tacgggttgg ctgtggtgcg cagcctggag ttcgcgcccg gcgacctctc
     1501 gccgtacggg gaggtgagct catccgagga ggcgtacttc gcggacgaaa ccgcgtacgc
     1561 cgtgcggggc gtcgacccgt cagtcgcgct ggtggtccgg ctcgaaccgg gactggcgga
     1621 cgacaccggg ccgtgggggg agtggatgct gctcacgcgt ggaccggcgc cgaacagcct
     1681 gtgtccgtac tacgaccccg aatcgtcgtt cacgccgcgc gactgcctgg agcatggcga
     1741 atgagccgga ccaggctggc ggcgatcggc gctgccgcgc tggccgcact ggccgggctg
     1801 gtcgcctaca tcgacggcga ccatgacatc gcgccgttct tcgtggcgct cgcgctggct
     1861 ggcgggatcc aggccgcaat cgtgcggcag ccgttccgtg gtggacggcg tgccgtcgcc
     1921 tgggccatcg ccggcggctg gctcatcgcc gccgtctgga tcggcgcgct gctggcgtgg
     1981 tggcaggcaa tgtgtgcctg ctcctacccg ccccggtcac cggaggaaac ctacctggga
     2041 ctgaccgcga cggcctacca cctcatcggc gtgtacggag gcgccgcgtt ggtgatcgcc
     2101 tcggcggtaa cagccgagct cagacgccga gcgggttgat cggcttggcg ccggtgccaa
     2161 ccaggatggc ttt
//
LOCUS       DATACA010000222         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2679680, whole genome shotgun sequence.
ACCESSION   DATACA010000222 DATACA010000000
VERSION     DATACA010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679680"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            778..1746
                     /locus_tag="VHK28_04580"
     CDS             778..1746
                     /locus_tag="VHK28_04580"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX2141540.1"
                     /translation="MNVNRGLLFWALALITAGVVALAVQQGLLDRDTVAGAWRLWPVI
                     LIAIGLSILLARTPFAVVGTLVAALVLGVVAGALVSVGPFVGGCGGDTPTQLAEESGS
                     FAGATASVTLDFNCGELDVAMTEGNAWETLTGAAGDDQPRVEGSGDALTVRSPSGEGF
                     DFGEGRQRWEIALGSEVTYDLSINPNAGDIDLDLSGGQFSELDVNPNAGALRFDLTST
                     TIEDFGLNMNAGSVDITAEADSRISGSIGMNAGAVDLCVPSDVPMRFEVEGAIFGHNL
                     DDDEHGLEQVGETFTSPGFNEADAVIDLRIEGNAASFNLNPEGGCE"
     gene            1743..2231
                     /locus_tag="VHK28_04585"
     CDS             1743..2231
                     /locus_tag="VHK28_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX2141541.1"
                     /translation="MNRRLYRSRTDSVIGGVAGGVAEYLDVDPSIVRIVWAILAIVTG
                     GIFFVLYIVMWIVVPEGPAQYAGPMPPPAAEGDSAEMPAGEPAASGQPAWTAPQEPPP
                     HRGSSVAPMVFGLLLIGLGAWFLLDEYVPWLDSDLLWPIGLVALGVLLLVVSMRRSST
                     AE"
     gene            complement(2490..2684)
                     /locus_tag="VHK28_04590"
                     /pseudo
     CDS             complement(2490..2684)
                     /locus_tag="VHK28_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
BASE COUNT          446 a          915 c          968 g          437 t
ORIGIN      
        1 ctcttggtta tgactgcttg tgcaccgatt tctggcggac atggaacgag ccgcgatccc
       61 tcgcggatcc gcggctcggg tcacgcccgg gtgggcatga aaaaaccgtg gacccgatct
      121 ggcccacggc tcgtggctgt cgtctggcgg ccttgccgcc gcctcgtcag ctcgaggagg
      181 gcacagatct cccgtcgaaa ccgccgttac ccgacggagt cgtggggcgc ttgttggttg
      241 cgcaccgcat gagatccatt gcctgaagct cttcctcgct aggtcgttga gtggccggac
      301 ggcatgacga accgcggatg gttcctctgg ccgccgcggg agtctacgac cgcgcccatt
      361 cccctgtcaa gaagaagccg cggttgccgt gccgcaccgg tacggcagga tgtacacgat
      421 cgcccgactc cagaccgata tcgtcgggaa atgtggtacg ggtgaggcgt tacctcgacg
      481 aacccagcca taggcgtgcg ggccggtgca cggagcccat gaatcgtggc cgttacgcag
      541 cacccgtacg gcatttccga acgaaatgct gccggcccgg gggcgacacc ggacatcgtt
      601 gcgatgctgc agcaccggtg ccgcattcgc gacgcggcgg gcggccagga acgcaccaac
      661 ctgccgacag ccctggagca acgtgtcctt tggcacggtt tgggtgggat tggggtgcaa
      721 caattgacgg tagcgttccg ggttgcgaac gccccgagcg acggaaggta atgagcaatg
      781 aacgtcaacc gcgggctcct attctgggcc cttgccctga tcacggcagg tgtcgtggcg
      841 ctggccgtgc agcagggcct cctggaccga gacacggtgg ccggcgcctg gcggctgtgg
      901 ccggtgatcc tgatcgccat cggtctctcg atcctgctgg cgcgcacgcc gttcgcggtg
      961 gtcggcacgc tggtggcagc gctggtcctg ggcgtggtgg ccggggcgct ggtgtcggtg
     1021 gggccgttcg tcggcggctg cggcggggat acgcctacgc agctggcgga ggaatccggc
     1081 agcttcgccg gcgccacggc cagcgtgacg ctggacttca actgcggcga gttggacgtg
     1141 gccatgaccg agggcaacgc ctgggagacg ctcaccggcg cggccggcga tgaccagcca
     1201 cgtgtcgagg gtagcggcga tgcgctgacc gtccgctctc cgagcggcga aggcttcgac
     1261 ttcggcgagg gccgccagcg ctgggagatc gcgcttggct cggaggtgac ctacgacctg
     1321 tcgatcaacc ccaacgccgg cgacatcgac ctcgatctgt ccggcggcca gttctcggag
     1381 ctggacgtca accccaacgc cggcgcgctc cgattcgacc tgaccagcac gaccatcgag
     1441 gacttcgggc tgaacatgaa cgccggctcg gttgacatca cggcggaggc ggactcgcgg
     1501 atcagcggca gcatcggcat gaacgccggc gccgttgacc tgtgcgtgcc gtcggacgtc
     1561 ccgatgcgct tcgaggtgga gggcgccatc ttcggccaca acctggacga cgacgagcac
     1621 ggcctggagc aggtcgggga gaccttcacc agcccgggct tcaacgaggc tgacgcggtg
     1681 atcgaccttc gcatcgaggg caatgccgcc agcttcaacc tcaaccctga aggaggctgt
     1741 gaatgaaccg gcgcctgtac cgatcccgca ccgactcggt catcggcggc gtggccggcg
     1801 gcgtcgccga gtacctcgac gtcgacccgt ccatcgtccg catcgtgtgg gccatcctgg
     1861 ccatcgtgac cggcgggatc ttcttcgtgc tctacatcgt gatgtggata gtcgtcccgg
     1921 agggtcccgc gcagtacgca ggaccgatgc ctccgcctgc ggcggagggc gatagcgcag
     1981 agatgccggc cggcgagccg gccgcatcgg gccagcccgc gtggaccgct ccgcaggagc
     2041 cgccgccgca tcgcggaagc tcggtggcac ccatggtctt cggcctgctg ctaatcgggc
     2101 tgggggcctg gttcctgctc gacgagtacg tcccgtggct ggactctgac ctgctgtggc
     2161 cgatagggct cgtggccctg ggggtcctgc tcctggtcgt ctcgatgcgg aggtcctcca
     2221 ccgccgaata ggtcgcgccc gaacagcgcg aaggcggccg cgcccacgcc caccaggacg
     2281 ccgagcgcca gcttcaggct gtccgcgctg atcaggtcgc cgcgagcccc ctgcgggggg
     2341 tccgggaagc ggaacgcgaa gttccacggc ggcagggtgc gcatcatgtt gatgtaccag
     2401 tccggcatgg ccagggccga gcccatgggg aagatgatca ccccggcagc caccgccagc
     2461 accaggaagg cgatggccaa cggccgccag ctccacgagc gcagcgcctc gtcgagcacg
     2521 taggccaccg ccaccatggc gaacatcagc gcggtcaggt agtggtactg gaaggcggcg
     2581 cgctcgatgc gcgtccaggg cagccactgg aaggcgaagg ccaccaccag cagcaccagc
     2641 gccagcgagc ggcggcgcca ggccatgatc ccgcagtaca ccatggccgg cacgccagcc
     2701 cagaacagga tcgggttgcc gccgttgtag atgaccccga tctgccggtc gtcgaagtcg
     2761 tgggtg
//
LOCUS       DATACA010000223         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1555757, whole genome shotgun sequence.
ACCESSION   DATACA010000223 DATACA010000000
VERSION     DATACA010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555757"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VHK28_04595"
     CDS             <1..295
                     /locus_tag="VHK28_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141542.1"
                     /translation="IALTSVAIPIFMILLSVTLPARVSRAMNLVVATLLIPYSVFNLA
                     GGEWMAFYGLGVALEVLLLAFILRSAWTWPRLASSRTVVDRAATPHALTTSAG"
     gene            641..1246
                     /locus_tag="VHK28_04600"
     CDS             641..1246
                     /locus_tag="VHK28_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2141543.1"
                     /translation="MKLTATMMLSVDGVYQGPGGPDEDRRGGFERGGWVAPHFDAETG
                     LFVTSVFERADALLLGRRTWEIFEGYWPHHDQGDPVSHGINVLPKYVPSSTLKDPGWN
                     TQVIDGDVEAAIRELKAKPGRELQVHGSGELLRWLLERDLVDELVLQIYPVVVGDGQR
                     LFPQRGQTHNLALAQSRSTPSGVMLQTYRPSGRATFGIAGE"
     gene            complement(1211..2533)
                     /locus_tag="VHK28_04605"
     CDS             complement(1211..2533)
                     /locus_tag="VHK28_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141544.1"
                     /translation="MTNVATTAGDRNSPRPFMPSRSRLPLPLGLLVLANAVSLSGNVI
                     TTVAIPWLILTTTGSAGLAGIAIFAGAGAAAAGGLAAGRIVDAIGPVRTSSAADLISG
                     LAVAPLPILLALGALEIWHVVLLSIVGTLADSAGSTARQAMVPAAADSGGYQRERANA
                     LFTSAEHMGYLLGAPVAGLLIAAFGVGGALWVTVASFALAALVVGGLVRLPRPQLTST
                     KTDGAGLRETIAFIWNDPALRALVVFPTMAVLLVGPLVPLVLPVLAREAFGDPVVLGL
                     MVASYGAGGLLGAAGFGMIGSRVPRRNLYTGVFVVWPSTYAAITLVPFLPFTLAMLVT
                     LGAAAGSLVPLQATIRQERSPARLLPRVVGLSTASVPVVGPIGVLVTGFLIDGLGLRQ
                     ALLLMTAGAALIGATVLTSKGTRSFDRGGPMAPVSSGLLPGDAERRAA"
     gene            2604..>3343
                     /locus_tag="VHK28_04610"
     CDS             2604..>3343
                     /locus_tag="VHK28_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX2141545.1"
                     /translation="MKEPADLDLRLLRSFVAVAEELHFTRAANRLYVAQQALSRDIAR
                     LESQMGARLFARSTRRVTLTPDGERLLVHARSILALNDAAIRELRGATRPLLVDVIGE
                     GTTAARIVDAARVAAPQFEFTVRYGGGLGAALPQLMAGRLDVAFGRTDGLGVSFPAAL
                     ARRLIRYEPLALLVPDNHPLAALPEVPCERLRGVEVDASAGNEDAPEWVDLAISLLDA
                     FGGRPSAPHPHVIGTTETAKHLEAHGLP"
BASE COUNT          540 a         1184 c         1142 g          477 t
ORIGIN      
        1 gattgcgctc acgagcgtgg cgatcccgat cttcatgatc ctgctctcag tgacgctgcc
       61 cgcccgtgtc agccgcgcca tgaacctcgt cgtggcaacg ctcttaatcc cctactcggt
      121 gttcaacttg gcgggcgggg aatggatggc cttctacggt ctcggcgtcg cactcgaggt
      181 gctcctcctg gccttcatcc tgcgctccgc ttggacctgg ccgcggctcg cctcctcccg
      241 aacagtggtg gaccgcgcgg ctacgccaca cgcgctcacc acgtctgctg ggtaagcgtc
      301 gccaatcggc ctccgcgccg gcgagctact taccagacca acggctcatc ccacgtctcg
      361 tgtcgtgcgg atgggccgta gggctacgcc gaccgcaaca caatcgctgt ggtgccggga
      421 gagcgcccgg acggaggacc ggcaactttg cccagaaccc tctactaccg cgccaggcag
      481 ctcgcgatcc cgccgacgac ctcgctcttc acagccatca aaccggcatc ctacacgcgg
      541 tccctcacgg gccttggccc cggtgcggac gtgcgaccga taagcctgcg tccgatcggt
      601 cgtcctacca cggaagggat atagcaggag cactaagacc atgaaactga ccgccaccat
      661 gatgctgagc gtggacggcg tgtaccaggg cccgggcggt ccggacgagg accggcgggg
      721 cgggttcgag cgtggcggat gggtggcgcc gcacttcgac gcggagaccg ggctcttcgt
      781 gacctcggtc ttcgagcgcg ccgatgcgct gctgctcggc cgccggacct gggagatctt
      841 cgagggctac tggccgcacc acgaccaggg cgacccggtc tcgcacggca tcaacgtcct
      901 gcccaaatac gtgccatcca gcaccctgaa ggatcctggc tggaacacgc aggtgatcga
      961 cggcgacgtg gaggcggcca tccgcgagct caaggccaag cccggccgcg agctgcaggt
     1021 ccatggcagc ggcgagctgc tccgttggct gctcgagcgg gacctcgtcg acgagctcgt
     1081 cctgcagatc taccccgtcg tggtcggcga tggacagagg ctcttcccgc agcgcggcca
     1141 gacgcacaac ctggcgctgg cccagtcgcg gtccacgccg tccggcgtga tgctccagac
     1201 ctaccgaccc tcaggccgcg cgacgttcgg catcgccggg gagtaacccc gagctgaccg
     1261 gggccatcgg cccacctctg tcgaagctgc gggtcccctt gctggtcagg accgtcgcac
     1321 cgatcagcgc ggcgccggcg gtcattagca ggagcgcctg acggagcccg agtccgtcga
     1381 tcaggaagcc ggtgaccagg acgccgatcg gtccaaccac cggcacgctc gcggtggaca
     1441 gccccaccac ccgcggcagg aggcgggccg gtgaacgctc ctgtcggatg gtggcctgca
     1501 gcggcacgag cgacccggcg gcagctccga gcgtcaccag catggccagg gtgaacggga
     1561 ggaacggcac cagggtgatg gccgcgtagg tggacggcca gaccacgaac acgccggtgt
     1621 acaggttccg gcgcggcacg cgggagccga tcatcccgaa cccggctgct ccgagcagcc
     1681 cgccggcgcc gtagctggcg accatcaggc ccagcacgac cgggtcgccg aacgcctcgc
     1741 gggccagcac gggcagcacg agcgggacga gcggacccac cagcagcacg gccatcgtcg
     1801 ggaagacgac cagcgcgcgg agcgccgggt cgttccagat gaaggcgatc gtctcgcgca
     1861 gcccggcgcc atcggtcttt gtgctggtga gctgaggacg gggcaggcga accagcccgc
     1921 cgaccaccag cgcagcgagg gcgaacgagg ccacggtcac ccacagcgca ccaccgacac
     1981 cgaacgcagc gatcagcagc cctgcgaccg gagctccaag caggtagccc atgtgctcgg
     2041 cggaggtgaa cagggcgttc gcgcgttcgc gctggtatcc ccccgaatcg gccgccgcgg
     2101 gcaccatcgc ctgccgcgcg gtggagccgg ccgagtcggc cagagtgccg acgatggaca
     2161 gcaggaccac gtgccagatc tcgagcgcgc caagcgccag gaggatgggc agtggcgcca
     2221 ccgcgagccc gctgatgaga tcggcagccg agctggtgcg aacgggaccg atagcatcca
     2281 cgatgcgtcc ggccgcaagg ccaccggcgg ccgccgcccc ggcgccggcg aagatcgcga
     2341 tcccggccag ccccgcgcta ccagtcgtgg tcaggatcag ccacgggatg gcgaccgtgg
     2401 tgatgacgtt tccactcagc gatacggcgt tggccagcac caggaggccg agcggaagcg
     2461 ggagccgcga cctcgacggc atgaaggggc gtgggctgtt gcggtccccg gcggtggttg
     2521 cgacgttggt catcacgcac acgatccgtc cggtttcgcc cgcagtccaa cacctgttgg
     2581 cggcggatta acatcagcag cgcgtgaaag aaccagccga tctcgatctg cgccttctgc
     2641 gctccttcgt tgccgtcgcc gaggagctgc acttcacccg agccgccaat cgcctgtacg
     2701 tggcgcagca ggcgctgagc cgcgacatcg ctcggctgga gtcgcagatg ggcgcccgcc
     2761 ttttcgctcg gagcacgcgc cgcgtgacgc tgacgccgga cggcgagcgc ctcctggtcc
     2821 acgctcggtc gatcctggcg ctcaacgacg ctgcgatcag ggaactgcgt ggcgcgaccc
     2881 gtccgctgct ggtggacgtc atcggcgaag gcaccaccgc cgcccgcatc gtcgacgccg
     2941 cgcgggtggc ggctccgcag ttcgagttca cggtgcgcta cggcggaggc ctgggcgcgg
     3001 cacttccgca actgatggcc ggccgcctgg acgttgcctt cggcaggacc gacgggctcg
     3061 gggtgtcctt tccggctgcg ctggctcggc ggctgatccg gtacgagcca ctggcgctgc
     3121 tcgttcccga caaccatccc ctggctgccc ttcccgaggt cccctgtgag cggctgcgtg
     3181 gcgtggaggt ggatgccagc gccggtaacg aggatgcgcc cgagtgggtg gacctcgcga
     3241 tctcattgct ggatgccttc ggcgggcggc cgtccgcgcc gcatccgcac gtgatcggca
     3301 ccaccgagac cgcgaagcac ctcgaagcgc acggcctgcc gat
//
LOCUS       DATACA010000224         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1474808, whole genome shotgun sequence.
ACCESSION   DATACA010000224 DATACA010000000
VERSION     DATACA010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474808"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VHK28_04615"
     CDS             <1..244
                     /locus_tag="VHK28_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2141546.1"
                     /translation="PDRTLTIFEWFFAEPGTGPGWESMQQTIAFSDEIQQEDIVICEQ
                     VQRGLRSRAYDTGRFSAKRENGVYHFQNLVREFLGE"
     gene            complement(331..1050)
                     /locus_tag="VHK28_04620"
     CDS             complement(331..1050)
                     /locus_tag="VHK28_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2141547.1"
                     /translation="MRTNVPRALKALRVQQRLRQVDLGRIASLTRDAVSRAERGMLRG
                     MTLGSLARLADALNAELVVEIRWQGAELDRLIDRFHAMLVAAIARRLEGAGWLVRVEV
                     SFNDFGDRGRCDIVAWHPVTRTLVVVEGKTRLGNLQETLGRLDVKARLGSVIAEQLGW
                     GRPSTVVPALVLTEVGSNRRVLREHDELFRGFEVRGRAALAWLRRPSGAIRGLLWFES
                     PDADGARTDRSARARGRQPAG"
     gene            1280..2146
                     /locus_tag="VHK28_04625"
     CDS             1280..2146
                     /locus_tag="VHK28_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2141548.1"
                     /translation="MTDAPTRGAGKLTVALISEVFWEPDGTARLRERLAEAVERGADI
                     ALLPELPLNPWRPSTKEPRDEDAEPMDGPRATAQAEAAAEAGIGLVGGIIHRDPDSGQ
                     RTSRALVIDRSGKLVATYEKLHLPEEPGFWETSHYRPGSAAPNRIDAFGLPVGVQLCS
                     DINRPQGSHILGAQGTMAVLAPRATEEKTYQRWKVVFRAIALTGACYVLSVNRPHPED
                     GVLIGGPSVAVEPDGDMLVETTDRLALVTLDAEVIHRARVAYPGYLPIRARLYADAWS
                     EIAGTDGEPRQP"
     gene            complement(2150..2845)
                     /locus_tag="VHK28_04630"
     CDS             complement(2150..2845)
                     /locus_tag="VHK28_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141549.1"
                     /translation="MTGDTVECFNCGRANPSWAQVCRSCGVPMGAGVSRTQSSGPIPT
                     DRDSLISIGAGLGAVVLAILLGVFVSGVIPDAPPPPVETPSPRPSVSGVPSPSLSPSG
                     PVGEGSPAPTQALPGTVAFGTALDGSNQVTEPTDIFTPGTQFCHSISVTEPFGAPQVL
                     EEILKIGEGGELSVVQERTALTVPADGTVAGFCVNANGLINDWGVGQFVLRDYRPGET
                     PVLLAEGRFTLAQ"
     gene            3065..3520
                     /locus_tag="VHK28_04635"
     CDS             3065..3520
                     /locus_tag="VHK28_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX2141550.1"
                     /translation="MAEARSHSAARPRKRRARRAAPNGGGETFGDRFRAVSAAITQAL
                     GTPWALLAAAFVVMVWGASGPIFGFSDTWQLVINTGTTIVTFLMVFAIQNSQNRESKA
                     IHLKLDELIRSHPKARDRLMMEEEAPEKEVQRDEEAMKRRARRRKSGDS"
     gene            3618..3809
                     /locus_tag="VHK28_04640"
     CDS             3618..3809
                     /locus_tag="VHK28_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141551.1"
                     /translation="MPADQQDLPGFGVRMDLRKPEGFVLEEGLIVRTLAGPKVESDRE
                     FNLDINLEAVALIAEGIGP"
BASE COUNT          690 a         1407 c         1399 g          593 t
ORIGIN      
        1 tccggaccgc acgctgacca tcttcgagtg gttcttcgcg gagccgggga ccgggccggg
       61 gtgggagagc atgcagcaga cgatcgcctt ctccgacgag atccagcagg aggacatcgt
      121 catctgcgag caggtgcagc ggggcctgcg ttcgcgggcc tatgacacgg ggcggttcag
      181 cgcgaagcgc gagaacggcg tgtaccactt ccagaatctc gttcgcgagt tcctcgggga
      241 gtagccgggc gcgtcggaca actttcgcca gattcaggct agctgcgcat atatggcttg
      301 gagtgggcgt gcgactgccc cctgcggctc tcaaccggca ggctggcggc cacgagcccg
      361 tgcggacctg tctgtgcgcg ccccgtctgc atctggcgac tcgaaccaca gaaggccgcg
      421 gattgcaccg gacggccgtc gaagccatgc aagagcagcg cggccacgca cctcgaagcc
      481 gcggaaaagc tcgtcgtgct cgcgcaggac gcggcggttc gaaccgactt cggtaaggac
      541 aagtgccggc acgaccgtgg acggacgacc ccaacccagc tgctccgcta ttacggaccc
      601 cagccgcgcc ttcacgtcga ggcgcccgag ggtctcctgg aggttcccga gtcgggtctt
      661 tccctccaca accaccagtg ttcgcgtgac gggatgccac gcaacgatgt cgcaacggcc
      721 gcgatcaccg aagtcgttga agctgacctc cacgcgtacc agccagcccg cgccctcgag
      781 cctgcgcgca atcgcggcca ccagcatcgc gtgaaaccga tcgatcagtc ggtcaagctc
      841 ggcgccctgc caccggatct cgacgaccag ctccgcgttc agcgcgtcgg ccaatcgcgc
      901 aagtgacccg agggtcatcc cgcgcagcat cccgcgttcg gcacgggata ccgcgtcgcg
      961 ggtcaggctc gcgatgcggc ccagatcgac ctgccgtaac cgttgctgaa cgcgtagggc
     1021 cttgagcgcg cgagggacgt tggtgcgcat cgcccgagga tgggccacga gtgcttactg
     1081 cccagtgatc aactgcttat cgccaaatgc agacgggatg cgcgtcactt ctcgccgagg
     1141 gtcccggtac cgcctggtgc ggacgtgatc ctgcaggctg gcggcggcac gcaggatcca
     1201 gggccgcgac gcgcgccgca tatgaatctg gcgactggcg cgagcacctg cggccgctgg
     1261 agcggtgtac cctcccgcca tgaccgatgc acccacacgc ggcgccggaa agctgaccgt
     1321 cgcgctcatc tccgaggtct tctgggagcc cgacggcacg gcgcggctgc gcgagcgcct
     1381 ggccgaggca gtcgagcgtg gcgcggacat tgcgctgctt cccgagctgc cgctcaaccc
     1441 ctggcgcccc tcgaccaagg agccgcgcga cgaggacgcc gagccgatgg acgggccgcg
     1501 cgccaccgcg caggccgagg cggcggccga ggccggtatc ggtctggtgg gaggcatcat
     1561 ccaccgagac ccggactcgg ggcagcgaac cagccgcgcg ctggtgatcg accgcagcgg
     1621 gaagctggtg gccacctacg agaagctgca cctgccggag gagcccgggt tctgggagac
     1681 aagccactac cggccgggca gcgcggcacc gaacagaatc gacgccttcg ggctgccggt
     1741 cggcgtgcag ctgtgcagcg acatcaaccg gccgcagggc tcgcacatcc tcggcgccca
     1801 ggggaccatg gcggtcctgg ccccgagggc cacggaggag aagacgtacc agcgctggaa
     1861 ggtcgtcttc cgggcgatcg ccctgaccgg cgcctgctac gtcctctcgg tgaaccggcc
     1921 gcacccggag gatggcgtgc tgatcggggg gccatcggtt gcggtggagc cagatggcga
     1981 catgctggtc gagacgaccg atcgcctggc gctcgtcacc ctggacgccg aggtcattca
     2041 cagggcgcgg gtggcgtatc ccggctacct gccgattcgg gcgcggctct acgcggacgc
     2101 gtggtcggag atcgccggca ccgacgggga gccccgtcag ccctagtcct tactgcgcca
     2161 gggtgaaacg cccctcagcc agcagcaccg gagtctcgcc cggccggtag tcgcgcagga
     2221 cgaactggcc cacgccccag tcgttgatca ggccgttggc gttgacgcag aagccggcga
     2281 ccgtcccgtc tgccggcacg gtgagcgccg tccgttcctg cacgacgctc agctctccgc
     2341 cctcgccgat cttcaggatc tcctccagca cctgcggagc cccgaagggc tcggtcaccg
     2401 agatggagtg gcagaactgc gtgccgggcg tgaagatgtc ggtcggctcc gtcacctggt
     2461 tcgagccgtc cagggccgtc ccgaaggcca ccgtcccggg cagcgcctgc gtcggcgcag
     2521 gcgagccctc acccacgggt cccgacggcg acaggctggg cgacggcacc ccggagaccg
     2581 atggccgggg cgacggcgtc tcgaccggcg gcggcggggc atccgggatc acgcccgaga
     2641 cgaagactcc cagcaggatg gcgagcacca ccgctcccag accagcgccg atggagatga
     2701 gcgagtcgcg gtcggtcggg atcgggccgc tgctctgcgt gcgcgaaacc cctgcgccca
     2761 tgggcacgcc gcaggagcgg cagacctggg cccaggaggg gttggcgcgt ccgcagttga
     2821 agcactcgac ggtgtcgccg gtcatcgggc cggagcctag cacgcgtcag cccatcaggg
     2881 accgattccc cgcgtggccg gaccggcggc acacgccttg cactccaccg ggcaagcaac
     2941 cgcaagctca gcaagcatcg aaggaggtgt ccgtgggccg catcctgatt ggcgtggtca
     3001 tcggggtgat cctggtgatc tggctgctcg tctcctgcat gtcgaacctc ttctaggggt
     3061 tgacgtggcc gaggcacgtt cacacagcgc cgcacgaccc cggaaacgac gcgcccgccg
     3121 cgccgccccc aacggcgggg gcgagacatt cggcgaccgc ttccgggcgg tcagcgcggc
     3181 gatcacgcag gcgctcggca cgccctgggc gctgctggct gccgcgttcg tggtcatggt
     3241 atggggcgcc agtgggccga tcttcgggtt ctccgatacc tggcagctgg tgatcaacac
     3301 aggcaccacg atcgtcacct tcctgatggt cttcgccatc cagaacagcc agaaccgcga
     3361 gtcgaaggcc atccacctca agctcgacga gctgatccgc tcccatccga aggcgcgcga
     3421 tcgcctgatg atggaggagg aggcgccgga gaaggaggtg cagcgcgacg aggaggccat
     3481 gaagcgtcgc gcccggagac ggaagtcagg ggacagctag cgccagacgg cgaaggccgg
     3541 gccgccccgc agagcgcccc gggtgaaccg gaagacactt ccgtcgacgc ggtggcttgc
     3601 acgtcctggt tcaccccatg cccgctgacc agcaggacct gcccggtttc ggagtgcgta
     3661 tggacctccg taaaccggaa ggtttcgtgc tggaagaagg cctcatcgtt cgcacccttg
     3721 ccggtccgaa ggtggaatcg gaccgagaat tcaacctcga catcaacctc gaagccgtcg
     3781 cactcatagc tgaaggaatc ggtccgtgaa ggtgccacag ggtggctcga gctggacgtg
     3841 gagtatgcgc gagcccgcgg tcctgctatc actcccccga tagccgctcc gcgggctcac
     3901 tcattccccc aaacctgctc gtttgcacgc ggcgtgcctt ccccgaggaa tgggccggtc
     3961 ctgacaaggc agggggagcc tgaatgcccg gaccggcccg atcgagacgc taactcgctg
     4021 ctggtgcgat cgccagagta cggaggtacc gtcgccggcc ccacataccc aagctgccgt
     4081 agcctgttg
//
LOCUS       DATACA010000225         8863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1715995, whole genome shotgun sequence.
ACCESSION   DATACA010000225 DATACA010000000
VERSION     DATACA010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715995"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VHK28_04645"
     CDS             <1..1057
                     /locus_tag="VHK28_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2141552.1"
                     /translation="SGHKEIMLGNYDAASGSWKQNSGSRGIYAVMVFVNVCGTLPTTP
                     SDPGESVRVAGSDRFGTAAALSKASFPAGVAVAYVGTGSNFPDALAAGPAAVVANAPV
                     LLVYRDSVPSVTATELGRLKPGRIVVLGATGAVSDGVARALAKYATSGRVDRIAGGDR
                     YATAAKISQAHFSHASVAYVATGANFPDALSGGAAAGKDGGPILLVQRTEVPAATAAE
                     LKRLDPDKIVVLGSSSAVSDGVAAALAKLTGAVVTRVAGSDRYLTAVKASQAEHAANG
                     PSTVYVATGTAFPDGLTGSPVAGGLPGPLLLVKPYELPSAVATELKRLAPSKVVVLGG
                     TSAISSGVVNAINAAVP"
     gene            1112..1846
                     /gene="rph"
                     /locus_tag="VHK28_04650"
     CDS             1112..1846
                     /gene="rph"
                     /locus_tag="VHK28_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX2141553.1"
                     /translation="MTTIAQSRRPDGRAPNQLRPVKIVADYLKFAEGSVLIRVGDTRV
                     ICAVTIDDRVPGWLRGKGSGWVTAEYSMLPRAGTERSQREASTGRVGGRTHEIQRLIG
                     RALRGVVDLPKLGERTITVDCDVIQADGGTRTASITGAYVALARALRKYGMAHLLTGQ
                     VAAVSVGMASGGVLLDLDYAEDSSADVDFNVVMTDDDRFVELQGTAEHRSFDRAQMNQ
                     MVDLAAAGIRQLFAIQRAAVEAPAAE"
     gene            1843..2442
                     /locus_tag="VHK28_04655"
     CDS             1843..2442
                     /locus_tag="VHK28_04655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX2141554.1"
                     /translation="MTDLLLATRNRGKQQEFRRLLAGVSGRVVVPQEIGLDLEVPEPH
                     DTYAENASAKARAFCRVAQLPVLADDSGIEVAALDWGPGVRSARFGEPGRDGADLLLE
                     RLAGASDRRARMVCWLALAVPPPTAADEPHIELFSGMVEGEVAPDRRGTGGFGYDPIF
                     LLPTGLTTAELGEGEKDRISHRGRAVAAALPRLRQILDG"
     gene            2644..5562
                     /locus_tag="VHK28_04660"
     CDS             2644..5562
                     /locus_tag="VHK28_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2141555.1"
                     /translation="MKSWRPLVSALVLFSLLATPAAARDGDAAPAVVFADSGEPQSAD
                     AKKEAAEPPRHASDRVIVRWKDAGRTSSALRRHGLSAKAALHAPHTSLVATNGRSVAD
                     VVAELSHDPDVAWVEPDYEIHVMGGPDDATVSGVAVNDALTNDQYSLDRMWVRDAWAI
                     STGASNVIAVLDTGVWAGHQDLAGKLVSGYDFVNDDTNAADDNAHGTWVAGIIAARVN
                     DGYGVAGISWSDKIMPVKVMNANGSGYTSDLVDGIRWAADHGADIINMSIGGYAASST
                     LHEAIKYAYAKDVVLVGAAGNNRIEQSHYPASFPEVISVSATQADDEFTNWSNYGPLV
                     DVSAPGASVLTTNCDRNSVSSCMYAGRHIVISGTSFASPNVAGVAALVRARYPDWSNA
                     QVMSRIISTADDLGFSGWDKRYGHGRVNATRALGGSTAKPAASPGDWMEGNNTLASAQ
                     VVGLNQRWWPSIHPAGDIDVFAFDVPRPGRLDITVGAVVDTTRVAKSALPIDPVLHIY
                     GNNQHLIKTVDDPGSGSTTERTSIQITGAGRIYLRVANWLPNGNRQGYLMENKFVDNV
                     APAMTSRVPAPNASDVRFDQPMTITFSEAVSGVSASSVVLRKAGSVVPASVTYSSSAR
                     RATLTPSAPLAGDSAYSLSVTTAIKDVAGNSLPAQSWSFTTGKSPIRLAGADRYATAA
                     TISKFGFSAGIPVAYLATGAGFPDALAGGALAGKTGGPILLVTNGTLPPATAAELDRL
                     NPDTIVVLGGKSAVSDAVLNAAKGYATGGSVTRLHGADRYATAAAVSKHGFAEGVPVA
                     YVATGTGFPDALAGGPLAARTGGPILLVTRTTVPAATAAELARLKPAKIVVLGGSTAV
                     SDAVLNSLKGYAGGGNVTRLAGADRFATAAKVSASRWLADGPDTVLLATGSAFADALA
                     GGAYGGLLDAPLLLTGSGALPPATAAELARLNPTRVIILGGSGAVNDGILLQVHALFD
                     "
     gene            5633..6229
                     /locus_tag="VHK28_04665"
     CDS             5633..6229
                     /locus_tag="VHK28_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2141556.1"
                     /translation="MSQRPARIHPTADVAAQATIGPGTSIWNQAQVREGARIGAGCII
                     GKNVYVDAGVVIGDNVKVQNNVSLYHGVTVEDGVFIGPHVCFTNDRLPRAINPDGSLK
                     TDDDWEVSPILVRHGAALGANSTILPGVTIGRWALVGSGSVVTRDVADHELVAGNPAR
                     RIGSACSCGQPLRDAADGRPFEGACPRCGRPFPPEAAA"
     gene            6235..7311
                     /gene="wecB"
                     /locus_tag="VHK28_04670"
     CDS             6235..7311
                     /gene="wecB"
                     /locus_tag="VHK28_04670"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX2141557.1"
                     /translation="MTVVGARPQFVKAAPVSRRLRERHTELLVHTGQHYDDAMSASFF
                     RELEIPEPDVNLEVGSGPHGAQTADMMRGLEPVLVAERPDGVLVYGDTNSTLAAAVVT
                     AKLAYPYGARPWLAHVEAGLRSFNRAMPEERNRVVADHLADLLLAPTQAAMRNLSDEG
                     LAPRAVMVGDVMVDAYRWAEQHAERHLPELARQRPGYVLVTLHRAENVDSPERLCEWL
                     AALAVDRPVIFPVHPRTRAVMAAAGLEVPANVELSDPVGYLAMVALERNASAIATDSG
                     GVQKEAYLAGVPCITLRTETEWVETVEAGWNRVVGGDPAALRAALDDPRLMDRDRPRP
                     ALYGDGETADRIVAALEQMEASAG"
     gene            7332..8411
                     /locus_tag="VHK28_04675"
     CDS             7332..8411
                     /locus_tag="VHK28_04675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX2141558.1"
                     /translation="MGEEEKESVWSALESGALAQGSRVRELEERFAGLVGVPHAVATS
                     SGTTALHLALLGYGIGPGDEVITVPFTFMASATTIVHAGAQPVFVDVAERDFCMDPDL
                     VEAAITPRTRGIMPVSLYGQPADMPALAEIAERRGLALVEDAAQTHGAAVGERRSGSW
                     GAGCFSFYPTKNMTTGEGGMVTTADAELAERVRLLREHGMKVRYHHDTLGYNFRMTDI
                     HAAIGLAQLPKLPDYNRRRRQIAGRYDAELRGVITPHVRSGVTHVYHQYTIRVARRDE
                     FAERLRERGVGSGIYYPIPVHRQKPFVEMGYGDQSFPVTERLTEQVLSIPVHPSLTDA
                     EVGTVIEAVNAVAAELGPLSVEVPA"
     gene            8462..8695
                     /locus_tag="VHK28_04680"
                     /pseudo
     CDS             8462..8695
                     /locus_tag="VHK28_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(8752..8863)
                     /locus_tag="VHK28_04685"
                     /pseudo
     CDS             complement(8752..8863)
                     /locus_tag="VHK28_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
BASE COUNT         1292 a         3223 c         3154 g         1194 t
ORIGIN      
        1 cagcggccac aaggagatca tgctcggcaa ctacgacgcg gcgtcgggca gctggaagca
       61 gaacagcggc tcgcgcggca tctacgcggt catggtcttc gtcaacgtgt gcggcaccct
      121 gccgaccacg cccagcgatc cgggcgagag cgtccgggtc gccgggtcgg accgcttcgg
      181 gacggcggca gccctgagca aggcgagctt cccggccggc gtcgcggtgg cgtacgtcgg
      241 caccggctcc aacttccccg acgcgctggc ggccggcccg gccgcggtcg tggccaacgc
      301 accggtcctc ctcgtctacc gtgactcggt cccgtcagtc accgccaccg agctcggccg
      361 cctcaagccg ggacgcatcg tggtgctggg ggccaccggc gcggtgtcgg acggcgtggc
      421 cagggccctg gccaagtacg ccaccagcgg cagggtggac cgcatcgccg gcggcgatcg
      481 gtacgccacc gcggccaaga tcagccaggc ccacttcagt cacgcttctg tcgcctatgt
      541 ggccaccggt gccaacttcc ccgatgccct gtccggcggc gcggctgccg gcaaggacgg
      601 cgggccgatc ctgctcgtcc agcggacgga ggtcccggcc gccacggccg ccgagctgaa
      661 gcgcctcgac ccggacaaga tcgtggtgct cggcagctcc tcggcggtgt cggacggcgt
      721 tgccgctgcc ctggccaagc tgaccggtgc cgtcgtgacg cgcgtggctg gctccgaccg
      781 ctacctgacc gcggtcaagg ccagccaggc ggagcacgcg gcgaacgggc cttccacggt
      841 gtacgtggcg accggcaccg ccttcccgga cggcctcacc ggcagcccgg tggccggcgg
      901 cctgcccgga ccgctgctgc tggtcaagcc ctacgagctg ccgtccgcgg tggcgaccga
      961 gctgaagcgg ctggcaccct cgaaggtcgt cgtgctgggc ggcaccagcg cgatctccag
     1021 cggcgtggtg aacgccatca acgcggcggt gccgtagccg gcgcccaagt cgccgtccag
     1081 tcatgggcca ggcgctagaa tcgtcaggcc catgaccacc atcgcccagt cgcgccgtcc
     1141 cgacgggcgc gcccccaacc agctgcgccc ggtcaagatc gtggccgact acctgaagtt
     1201 cgccgaggga tcggtcctga tccgcgtggg cgacacgcgc gtcatttgcg cggtgaccat
     1261 cgacgatcgg gttccgggct ggctgcgggg caagggcagc ggctgggtga ccgccgagta
     1321 cagcatgctg ccgcgggccg gcactgagcg cagccagcgc gaggcgagca ccgggcgcgt
     1381 cggcggccgg acgcacgaga ttcagcgcct gatcgggcgg gcgctgcgcg gggtggtgga
     1441 ccttcccaag ctcggggagc ggaccatcac cgtcgactgc gacgtcatcc aggccgacgg
     1501 cggcacgcgg accgccagca ttactggcgc ctacgtcgcc ctggcgcgcg cgctgcgcaa
     1561 gtacggcatg gcacacctgc tcactggcca ggtcgcggcg gtcagcgtcg ggatggcctc
     1621 gggtggcgtg ctgctcgacc tcgactacgc ggaggactcc agcgcggacg tcgacttcaa
     1681 cgtggtcatg accgatgacg accgcttcgt ggagctgcag ggcaccgccg agcatcggtc
     1741 gttcgaccgg gcgcagatga accagatggt ggacctggcc gccgccggca tacgacagct
     1801 gttcgccatc cagcgtgccg cggtcgaggc gcccgccgcc gagtgaccga cctgctgctg
     1861 gcgacccgca accgcggcaa gcagcaggag ttccgccgcc tgctggccgg cgtttcgggc
     1921 cgcgtcgtgg tcccgcagga gatcggtctc gacctcgagg tccctgagcc tcacgacacc
     1981 tacgccgaga acgcgtcggc caaggcacgc gccttctgcc gggtggcgca gctgccggtg
     2041 ctggccgacg actcgggcat cgaggtcgcc gcccttgact ggggcccggg cgtgcgcagc
     2101 gcgcgcttcg gcgagccggg tcgcgacggc gccgacctgc tgctggaacg cctggcgggg
     2161 gccagcgatc ggcgcgcgcg gatggtctgt tggctggcgc tggcggtacc accaccgacg
     2221 gcggcggacg agccgcacat cgagctgttc agcggcatgg tggaaggcga ggttgcgcca
     2281 gaccgccggg gaacgggtgg cttcggctac gacccgatct tcctgctgcc gaccgggctg
     2341 accaccgccg agctggggga gggggagaag gaccgcatct cgcaccgcgg acgggcggtt
     2401 gccgccgcgc tgccccgcct ccgtcagatc ctggacggct gaggccagcg ccgatggaat
     2461 gacccgctgg ccctgcccgc gggggtgtgg ggcatgggcc gtcagtcccc gccacggggg
     2521 agccgatggt catctttgct tgcccgccgt gcgcccatac gctgcacgct gcgatctggg
     2581 cacgggcgca atagaccgtc cggcctcgtc caaaccgaca ccaagtccgg agcccgagcc
     2641 ctgatgaagt cctggcgccc tctcgtctct gccctggtcc tgttcagcct gctggccacg
     2701 ccggccgcag cacgcgacgg ggacgcggcc ccggccgtgg tcttcgccga ctccggcgag
     2761 ccccagtcgg ccgacgccaa gaaggaggcc gccgagccgc cgcgccatgc ttccgaccgg
     2821 gtgatcgtgc gctggaagga cgcgggccgc acgagctcgg ccctccgccg ccacgggctc
     2881 tcggccaagg cagcgctcca cgccccgcac acctcgctgg tcgccaccaa cggccgctcc
     2941 gtggctgacg tggtcgccga gctcagccac gacccggacg tggcgtgggt tgagccggac
     3001 tacgagatcc atgtgatggg cgggcctgat gacgccaccg tcagcggcgt ggcggtcaac
     3061 gacgcactga ccaatgacca gtactcgctc gaccgcatgt gggtgcgcga cgcgtgggcc
     3121 atcagcaccg gcgcctcgaa cgtgatcgcc gtgctggaca ccggggtgtg ggccggccac
     3181 caggacctgg ccggcaagct ggtgtcgggg tacgacttcg tcaacgacga caccaatgcc
     3241 gccgacgaca atgcccacgg cacctgggtg gccggcatca tcgcggcgcg tgtcaacgac
     3301 ggctacggcg tggccggcat cagctggtcc gacaagatca tgccggtcaa ggtcatgaac
     3361 gccaacggca gcggctatac ctccgacctg gtggacggca tccgctgggc ggccgaccac
     3421 ggcgccgaca tcatcaacat gagcatcggc ggctacgccg cctcctcgac gctccacgag
     3481 gcgatcaagt acgcctacgc caaggacgtg gtgctggtgg gtgcggccgg caacaaccgc
     3541 atcgagcaga gccactaccc ggccagcttc ccggaggtca tctcggtgag cgccacccag
     3601 gcagatgacg agttcaccaa ctggtcgaac tacgggccgt tggtggacgt ttcggcgccg
     3661 ggcgcatcgg tcctgaccac caactgcgac cggaattcgg tgagctcctg catgtacgcc
     3721 gggcgtcaca tcgtgatcag cgggacctcc ttcgccagcc cgaacgtcgc cggcgtggcg
     3781 gccctggttc gggcgcgcta ccccgattgg agcaacgcgc aggtcatgtc gcgcatcatc
     3841 tccaccgccg acgatctcgg gttcagcggc tgggacaagc gctacgggca tggtcgggtg
     3901 aacgccaccc gcgccctggg cggcagcacc gccaagccgg cggccagccc cggcgactgg
     3961 atggagggca acaacaccct cgccagtgcc caggtggtgg gcctcaacca gcgctggtgg
     4021 cccagcatcc atcccgccgg cgacatcgac gtcttcgcct tcgacgtgcc gcgccccggc
     4081 cggctggaca tcaccgtcgg cgccgtcgtg gacaccaccc gggtggccaa gagcgcgctg
     4141 cccatcgatc ccgtgctgca catctacggc aacaaccagc acctgatcaa gacggtcgac
     4201 gacccgggca gcggctcgac aaccgagcgg acgtcgatcc agatcaccgg cgccggtcgc
     4261 atctacctgc gggtcgccaa ctggctgccc aacggcaacc ggcagggcta cctgatggag
     4321 aacaagttcg tcgacaacgt cgccccggcc atgacctccc gggttcccgc gcccaacgcc
     4381 agcgacgtgc gcttcgacca gccgatgacc atcaccttca gcgaggcagt cagcggcgtg
     4441 agcgcgtcga gcgtggtgct gcgcaaggcg ggcagcgtgg tgccggccag cgtcacctac
     4501 agctcttccg ctcggcgtgc caccctcacc cccagcgcgc cgctggcggg tgacagcgcc
     4561 tactcgctga gcgtcacgac cgcgatcaag gacgtcgccg gcaactccct gccggcccag
     4621 tcctggagct tcaccaccgg caagtcgccg atccggctgg ctggcgcgga tcgctatgcc
     4681 acggcggcga ccatcagcaa gttcggcttc tccgcgggca tcccggtggc gtacctggca
     4741 accggggccg gcttcccgga tgccctggcc ggcggcgccc ttgccggcaa gacgggaggc
     4801 ccgatcctgc tcgtcaccaa cggcacgctg ccgcccgcca ccgccgccga gctcgaccgt
     4861 ctcaatcctg acacgatcgt ggtcctgggc ggcaagagtg cggtgagcga cgccgtcctc
     4921 aacgccgcca agggctacgc caccggcggc agcgtcaccc gcctgcacgg tgccgaccgc
     4981 tacgccaccg ccgcggcggt cagcaagcac ggcttcgccg aaggcgtgcc ggtggcctac
     5041 gtggcgaccg gcaccgggtt cccggacgcg ctggccgggg gtccgctggc ggccaggacc
     5101 ggcgggccga tcctgctggt cacccgcacc acggtgcccg cggcgacggc cgccgagctg
     5161 gctcggctga agccggccaa gatcgtggtc ctgggcggca gcaccgccgt gagcgacgcg
     5221 gtgctgaaca gcctgaaggg ctacgccggc ggcggtaatg tcacgcggct ggcgggtgcg
     5281 gaccgcttcg ccacggcggc caaggtgagc gccagtcgct ggctggcgga cggtcctgac
     5341 accgtcctgc tggccaccgg ctcggcattc gccgacgccc tggccggggg tgcctacggc
     5401 gggctgctcg acgccccgct gctgctcacc ggctcgggcg cgttgccgcc ggcgaccgcc
     5461 gccgagctcg cccgcctgaa cccgacgcgg gtcatcatcc tgggcggctc gggcgcggtg
     5521 aacgacggga tcctcctcca ggtgcacgcc ctcttcgact gacctcccgg gggggcacac
     5581 ctgggaccgg tcgagccgcc tcggctagaa tcggcgcggc caaggaggtc gcatgtccca
     5641 gcgccccgcg cgcatccacc ccaccgccga tgtcgccgcg caggcgacca tcggtcccgg
     5701 cacgagcatc tggaaccagg cgcaggtgcg cgagggcgcc cgcatcggtg ccggatgcat
     5761 catcggcaag aacgtgtacg tggatgccgg cgtggtcatc ggtgacaacg tcaaggtgca
     5821 gaacaacgtg agcctgtacc acggcgtgac cgtcgaggat ggggtcttca tcggtccgca
     5881 cgtgtgcttc acgaacgatc ggcttccgcg cgccatcaat cccgacggca gcctgaagac
     5941 cgatgacgac tgggaggtct ctcccatcct ggtccgccac ggtgcggcgc tgggagccaa
     6001 cagcaccatc ctgcccggcg tgaccatcgg gcgctgggca ctggtcggct cgggctcggt
     6061 ggtgacccgc gacgtggccg atcacgagct ggtggccggc aacccggcgc gccgcatcgg
     6121 ctcggcctgc tcctgcggcc agcccctgcg cgacgcggcg gatgggcggc ccttcgaggg
     6181 tgcctgtccg cgctgcggcc gtcccttccc cccggaggct gcggcgtgaa ggtcatgacc
     6241 gtggtcggcg cccggccgca gttcgtgaag gcggcgcccg tcagccgccg cctgcgggag
     6301 cggcacaccg agctgctggt gcacaccggg cagcactacg acgacgccat gagcgcctcg
     6361 ttcttccggg agctggagat cccggagccg gacgtgaacc tggaggtggg ctcgggtcca
     6421 cacggggcac agaccgccga catgatgcgc ggcctggagc cggtgctggt cgccgagcgg
     6481 ccggacgggg tgctggtcta cggcgacacc aactccacgc tggccgcggc agtggtgacc
     6541 gccaagctcg cctaccccta cggcgcccgt ccctggctgg cgcacgtgga ggccgggctg
     6601 cgctcgttca accgggccat gcccgaggag cgcaaccgcg tggtcgcaga ccacctggcc
     6661 gacctgctgc tggcccccac ccaggcggcc atgcgcaacc tctccgacga ggggctggct
     6721 ccgcgggcgg tgatggtcgg cgacgtgatg gtcgacgcct atcgctgggc ggagcagcac
     6781 gccgagcgtc acctgcccga gctggcgcgc cagcgcccgg gctacgtgct ggtcacgctt
     6841 caccgcgcgg agaacgtcga cagcccggag cggctgtgcg agtggctggc ggcgctggcc
     6901 gtcgaccgcc cggtcatctt cccggttcac ccgcgaacgc gggccgtcat ggcggccgcc
     6961 gggctggagg tgcccgccaa cgttgagctg agcgacccgg tcggctacct ggccatggtc
     7021 gccctggagc gcaacgccag cgccatcgcc accgactcgg ggggtgtcca gaaggaggcc
     7081 tacctggccg gcgtgccctg catcacgctg cgcaccgaga ccgagtgggt cgagacggtg
     7141 gaggccggat ggaaccgggt ggtgggcggc gacccggccg cactgcgcgc cgccctggac
     7201 gacccgcggc tgatggaccg cgaccggccg cgcccggcgt tgtacggtga cggggagacg
     7261 gccgaccgca tcgtggcggc cctggagcag atggaggcct cagcagggtg atacccatcg
     7321 cgcgcccaca gatgggcgag gaggagaagg agagcgtctg gtcggcgctg gagtccgggg
     7381 cgctggccca ggggtcccgc gtccgggagc tggaggagcg gttcgccggc ctggtcggcg
     7441 tgccacacgc ggtcgccacc agctcgggca ccacggccct gcacctggcg ctgctgggct
     7501 acggcatcgg tccgggcgac gaggtcatca cggtcccctt caccttcatg gccagcgcca
     7561 ccaccatcgt gcacgccggg gcgcagccgg tgttcgtcga cgtggccgag cgcgacttct
     7621 gcatggaccc agacctggtg gaagccgcca tcacgccccg gacgcggggc atcatgccgg
     7681 tcagcctcta cgggcagccc gccgacatgc cggcgctggc cgagatcgcc gagcgccgcg
     7741 gcctggcgct ggtggaggat gccgcccaga cgcatggcgc ggcggttggc gagcgccgct
     7801 caggctcctg gggcgccggc tgcttcagct tctacccgac caagaacatg accaccggtg
     7861 agggcggcat ggtgaccacc gccgacgccg agctcgccga gcgcgtccgc ctgctgcgcg
     7921 agcacggcat gaaggtgcgc taccaccacg acaccctggg ctacaacttc cggatgaccg
     7981 atatccacgc cgccatcggg ctggcgcagc ttcccaagct gccggactac aaccgccgcc
     8041 ggcgccagat cgcggggcgc tacgacgccg agctgcgcgg cgtgatcaca ccccatgtcc
     8101 gcagcggcgt gacgcacgtc taccaccagt acacgatccg cgtggcgcgg cgcgacgagt
     8161 tcgcggagcg actgcgcgag cggggtgtgg gcagcggcat ctactacccg atcccggtcc
     8221 accgccagaa gcccttcgtc gagatggggt acggggacca gtccttcccg gtcaccgagc
     8281 ggctgaccga gcaggtgctc tccatcccgg tccacccgtc gctgaccgat gccgaggtgg
     8341 gcaccgtgat cgaggcggtc aacgcggtcg cggctgaact cggcccgctc tcggtcgagg
     8401 tgccggcgtg accgaggccc cgccgcgcgc gctgcgcgcc ggcgtggtgg ggctggggat
     8461 gatgggccgc aaccacgtcc gcgtctggga cgagggggtg gacggcgtac agctggtggc
     8521 ggtcgccgac ccggagccgg aggcgctgcg ccgggcgaca cagggccgct ccctggccgg
     8581 ctatccggac cccgacgcca tgttcgccgc cgagcagctg gacctcgtca gcgtggtcgc
     8641 cccgacctcg ctgcacctgc cgatcgtcga ggccgcgctg cgcgccggcg tcaacgtgct
     8701 ggtcgagaag ccgattgccg ccacccgcgc agaggcggag gccatgatgg cggcgatcgg
     8761 cttctcgacc agcacgttga cgccggcgcg cagcgcggcc tccacgatcg gcaggtgcag
     8821 cgaggtcggg gcgaccacgc tgacgaggtc cagctgctcg gcg
//
LOCUS       DATACA010000226         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_270438, whole genome shotgun sequence.
ACCESSION   DATACA010000226 DATACA010000000
VERSION     DATACA010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270438"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VHK28_04690"
     CDS             complement(<1..563)
                     /locus_tag="VHK28_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2141559.1"
                     /translation="MTDRYDLVIVGAGAGGEAAAFQARELGLSVAIVERDLFGGGCPF
                     WQCMPSKALLHAAAVHHAGGDYPWKRASDFRDWMINREGIDYPDDGGHVGQLEDAGAV
                     PIRGEARFLGADPLCLEVRTADGGRRELEAGSVILAVGSHSRIPSLPGLDEVGAWTNR
                     EGTSLRELPDGVVVLGGGPSGLELGQV"
     gene            complement(560..1738)
                     /gene="hutI"
                     /locus_tag="VHK28_04695"
     CDS             complement(560..1738)
                     /gene="hutI"
                     /locus_tag="VHK28_04695"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX2141560.1"
                     /translation="MPPSTRLFTGRGRLLTPEPFSGESPWAVHVTDGSINWVGARRDA
                     PASDDELDLGEALVTPGLVDAHTHPIFAGERADEAAARLAGQPYSGGGILRTVSATRA
                     ATDAELEDLAVRRLRAQLSAGTTTVEAKSGYGLSTQEELRHLRIIRVAAERSGARVVA
                     TFLGAHARPDDDPAYVDRIVDQMLPQVAAGELARFCDVFCDRGFFSVDEAERILRAAG
                     GHGLGLRLHAEQLSRSGAAELGVRLGATSVDHLEQLDADGVRTLAASRTVATLLPGPA
                     LVMRDRLPPARELLDAGAAVALASDANAGTFGAWGAMPMVIGLGATLLRMTVAEALTA
                     ATRGGASALGLQGRRGVIGPGADADLVAWDAEHEGAFALHLGNVRPATVVLAGSILPA
                     "
     gene            complement(1759..2700)
                     /locus_tag="VHK28_04700"
     CDS             complement(1759..2700)
                     /locus_tag="VHK28_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2141561.1"
                     /translation="MSPPTPRRTSSQDPFAALAGRPVLSAVLGALAIAFSGVLVRQAD
                     VAPATAAIFRCLYALPPLALLALAERRAYGPRPASQVGLAYLAGIFFAVDLELFHHTI
                     ALVGAGLATVLGNTQVVFVAVLAWLLLGERMTPRTAVAIPVVMIGVVLISGIIGADAY
                     GRDPVLGGITGLATGLSYAGFLLILRRGNADLRRPAGPLFDATLSASVAGVVIGLPLG
                     EVDLVPSWPAHLWLVLLALSAQVLGWLLISVSLPRLPAALTSVVLTLQPVGAVVLAVL
                     LVAETPSVVQLAGAACILAGLLLATVRRRAAPPAEFG"
     gene            2774..>3050
                     /locus_tag="VHK28_04705"
     CDS             2774..>3050
                     /locus_tag="VHK28_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2141562.1"
                     /translation="MRSLPTGTVTFLFTDIEGSTRLAQKLGEAWPPILERHNAVLREA
                     VELNGGVVFGTEGDALFAAFGSASDAVRASVAAQRALAAEPWPEGATV"
BASE COUNT          450 a         1116 c         1139 g          345 t
ORIGIN      
        1 tacacctggc cgagctccag cccgctcgga cccccgccca gcacgaccac cccgtccggg
       61 agctcgcgga gcgaggtgcc ctcgcggttc gtccaggctc cgacctcatc gagcccgggc
      121 agcgatggga tacgcgagtg cgatcccacg gccaggatca cgctgcccgc ctcgagctcg
      181 cgtcgaccgc cgtccgcggt tctcacctcc aggcacaggg gatcggctcc caggaagcgc
      241 gcctcgccgc ggatgggaac cgctccggcg tcctcgagtt ggccgacgtg gccgccgtcg
      301 tccggatagt cgattccctc gcggttgatc atccagtcgc ggaagtcgct ggcgcgcttc
      361 caggggtagt cgccgcccgc gtggtgcacc gccgccgcgt ggagcagcgc cttggagggc
      421 atgcactgcc agaacggaca gccgccgccg aacaggtcgc gctccacgat ggccacgctc
      481 agccccagct cccgcgcctg gaaggcggcc gcctcacctc cggcgcccgc gccgacgatg
      541 acgaggtcgt atcggtccgt cacgcgggaa ggatagagcc ggcgagcacc acggtcgcgg
      601 ggcggacgtt gccgaggtgg agggcgaagg caccctcgtg ctcggcatcc caggccacca
      661 ggtcggcatc ggctcctgga ccgatgactc cgcgccggcc ctgcaggccc agcgcggacg
      721 ccccgccacg ggtcgcggca gtgagcgcct cggccaccgt catgcgcagc agcgtggcgc
      781 ccaggccgat gaccatgggc atggcacccc acgccccgaa ggtgccggcg ttggcgtccg
      841 aggcgagcgc cacggcggcg ccggcatcca gcagctcgcg cgccggtggc agccggtcgc
      901 gcatgacgag cgccgggccg ggcagcaggg tggccaccgt gcgcgaggcg gccaacgtcc
      961 ggaccccgtc ggcgtcgagc tgctccaggt gatcgacgct ggtggccccc agccgcacgc
     1021 cgagctcggc cgcaccgctg cgcgagagct gctccgcatg cagccgcaat cccaggccgt
     1081 gcccgccggc ggcgcgcagg atgcgctcgg cctcgtccac ggagaagaag ccgcggtcgc
     1141 agaacacgtc gcagaagcgg gccagctccc cggccgcgac ctgcgggagc atctggtcca
     1201 cgatgcggtc gacgtaagcc gggtcgtcgt ccggccgggc gtgcgccccc aggaaggttg
     1261 ccaccacccg tgccccgctg cgctccgccg cgacacggat gatccgcagg tggcgcagct
     1321 cctcctgggt ggacaggccg tacccggact tggcctcgac ggtagtggtg cccgcgctca
     1381 gctgggcgcg caggcgacgg acggcgaggt cctccagctc cgcgtcggtc gccgcccgcg
     1441 tggcggacac cgtgcgcagg atgccgccgc cggagtacgg ctggccggcg agtcgggcgg
     1501 cggcctcgtc ggctcgctct ccggcgaaga tggggtgggt gtgcgcgtcg accaggccgg
     1561 gggtgacgag cgcctcgccg aggtcgagct cgtcgtccga ggccggagcg tcgcgtcgtg
     1621 cgccgaccca gttgatggag ccatcggtca catggaccgc ccacggggac tcgccggaga
     1681 agggctccgg ggtgagcaac cggccgcggc cggtgaacag gcgcgtgctg ggaggcatcg
     1741 gtctttacaa cggtaccctc agccgaactc ggccggtggc gccgcgcgtc ggcggacggt
     1801 ggccagcagg aggccggcca ggatgcaggc cgcacccgcc agctggacga ccgatggagt
     1861 ctcggccacc agcagcacgg ccaggaccac ggcgccaacc ggctgcaggg tcagcaccac
     1921 gctggtcagc gcggccggca ggcgggggag cgagacgctg atcagcagcc agccgagcac
     1981 ctgcgcggag agcgccagca gcacgagcca caggtgcgcc ggccaggacg gaaccaggtc
     2041 gacctcgccc agcgggaggc cgatgacgac gcccgccacg ctcgccgaca gggtggcgtc
     2101 gaagagcggg ccggccgggc gccgcaggtc ggcgttgccg cgacgcagga tcaggaggaa
     2161 ccccgcgtac gacagcccgg tcgccagacc ggtgatgccg cccagcaccg gatcgcggcc
     2221 gtaggcgtcc gccccgatga tcccggagat gagcaccacc ccgatcatca caaccgggat
     2281 ggcgaccgcg gtgcgaggcg tcatgcgctc gcccagcagc agccaggcga gcaccgccac
     2341 gaacacgacc tgggtgttgc ccagcacggt ggccagcccg gcgcccacca gggcgatggt
     2401 gtggtggaac agctccaggt cgacggcgaa gaagatcccg gccaggtagg ccaggcccac
     2461 ctgggacgcg gggcgtgggc cgtaggcacg ccgctcggcc agggccagca gggccagcgg
     2521 cgggagcgcg tacaggcagc ggaagatggc agctgtggcc ggcgctacgt ccgcctggcg
     2581 caccagcacg ccgctgaagg cgatggccaa tgccccgagc acggcggaca gcaccggccg
     2641 tccggccagg gcggcgaagg ggtcctgcga actggtgcga cgcggtgtcg gcgggctcac
     2701 gactgcggag cgtagcgcga ccggcccttc cgggggcttg ccctgcgggc cgctcacccc
     2761 gaaacttgcc tgcgtgcgaa gcctgccgac cgggacggtc accttcctgt tcaccgatat
     2821 cgaggggtcc acgcgcctcg cgcagaagct gggcgaggcc tggccgccga tcctggagcg
     2881 gcacaacgcc gtgctgcgcg aggccgtcga gctgaacggc ggggtcgtgt tcggcacgga
     2941 gggcgacgcg ctgttcgccg ccttcggcag cgcgtcggat gccgttcgag cctcggtggc
     3001 agcgcagcgc gccctcgccg cggagccctg gccggagggc gcgaccgtcc
//
LOCUS       DATACA010000227         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_752702, whole genome shotgun sequence.
ACCESSION   DATACA010000227 DATACA010000000
VERSION     DATACA010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752702"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..882
                     /locus_tag="VHK28_04710"
     CDS             154..882
                     /locus_tag="VHK28_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2141563.1"
                     /translation="MDVPRLPAVLRAIRIRKGWRQIDVARAAGVSRSMVGRAESPRIA
                     KLPLEAILRIATALDVRIDITARWKGGELDRMLNAGHAGMHEQVAGLLAGQPPWLFRP
                     EVSFAIYGERGVIDILAFHPPTGALLVIELKTELVDVQNVIGTMDRYTRLAARIAADL
                     GWKANQVSCWILFPDTSTNRRRVAAHSGVLRAAFPADGRTMRGWLDTPEGTIRALSFL
                     PPAKTVAAAGTGVRRVRRRVRSAA"
     gene            879..>2127
                     /locus_tag="VHK28_04715"
     CDS             879..>2127
                     /locus_tag="VHK28_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2141564.1"
                     /translation="MTDLIVAGGTVVTARRSFAADVAIKDGRIEAVGPALRRGSDART
                     LDATGLLVLPGVIDVHTHTRLPTDDEPDRFYQDSVAAAHGGTTTFLAFNNPGTGISDA
                     GASSLLAGLDEFTARTEGESAVDYALSSVISGQQPDPISELPELIARGVPTAKAFMVY
                     DFRLTDDRLFAAMTAMGRHGGMLQVHCENATIIDALTQGALHAGRTSCHHHAACRPTY
                     AEAEATHRAIALAQAAEASVYVVHLSCADALAEVAAAKARGWPVYAETCPHYLSRTDA
                     AYGAEDESEVIRSVISPPLRSEADRQALWLGLAHAGLDVVASDHVPDRLAVEKAVPAP
                     PFPQISNGAPGIETLLAVTYSAGPGAGRISLERMVDVLSTTPARLFGLEAKGTVEAGK
                     DADLVLFDPTSARTVTQESLHHTS"
BASE COUNT          344 a          750 c          729 g          304 t
ORIGIN      
        1 ttaaggattc cctggtgagc actagaccga gttccccggg caagattgga cgtaaggctc
       61 agcagccgag caattgacca agtcgacgcg acggcaccag cgacgcaact gcagtagtcg
      121 gctggtaaca acccgtcgcg cagaatcgcg gccatggatg ttccacggct tccggcggtg
      181 ctgcgcgcga tccggatccg gaaaggatgg cgccagatcg acgtcgcccg cgcggcagga
      241 gtatcgcgga gcatggtcgg tcgcgcagaa tcgccccgga tcgccaagct gccgctggag
      301 gccatcttgc ggatcgccac cgccctggac gtccgcatcg acatcaccgc acgctggaag
      361 ggaggcgagc tggaccggat gctcaatgcc ggtcacgccg gaatgcatga gcaggtggcg
      421 gggctgctgg cggggcaacc gccgtggctg ttccggccgg aggtctcgtt tgcgatctac
      481 ggcgagcgtg gggtgatcga catcctggcc ttccatccgc cgacgggcgc actgctggtg
      541 atcgagctca agaccgagct ggtcgatgtg cagaacgtga tcggcacgat ggaccgatac
      601 acccgcctcg ccgctcgcat cgccgcggac ctgggatgga aggcaaacca ggtcagctgc
      661 tggatcctct tccctgacac gagcacgaac cgacggcgcg ttgcggccca cagcggcgtc
      721 ctgcgagcgg cgtttccggc agacggccgt acgatgcggg gctggctgga cacgccggag
      781 gggacgatcc gggctctgtc cttcctgccg ccggcgaaga ctgtcgcggc ggccgggaca
      841 ggggtgcgga gggtgcggcg tcgggtacgc tccgctgcat gacggacctg atcgtggccg
      901 gaggcacagt ggtcactgcg cgacgctcgt tcgccgcgga cgtggcgatc aaggacgggc
      961 ggatcgaagc ggtggggccg gccctgcgac gaggttccga cgcacgcacc ctcgacgcca
     1021 cgggactgct ggtcctgccc ggcgtcatcg acgtgcacac ccacacccga ctgccgaccg
     1081 acgacgagcc ggaccgcttc taccaggaca gcgtcgcggc cgcgcacggc ggcaccacca
     1141 ccttcctcgc cttcaacaac cccggaacgg gcatcagcga cgcgggtgcc tcctccctgc
     1201 tggccggcct cgacgagttc actgcgcgca ccgagggtga atcggccgtg gattacgcgc
     1261 tgtcatcggt catcagcggt cagcagccgg acccaatctc ggagcttccc gagctaatcg
     1321 cccgcggggt cccgaccgcc aaggcgttca tggtctacga cttccgcctg accgatgacc
     1381 gcctcttcgc cgcgatgacc gccatgggcc gccatggcgg catgctgcag gtccactgcg
     1441 agaacgccac catcatcgac gccctgactc agggcgcgct gcacgccggt cggaccagct
     1501 gtcaccacca cgccgcctgc cgaccgacct acgcggaggc ggaggccacc catcgggcca
     1561 tcgcgctggc gcaggccgcg gaggcatcgg tctacgtggt gcacctgtcg tgtgccgatg
     1621 cgctggcgga ggtcgcggcc gccaaggcgc ggggctggcc agtctacgca gagacctgcc
     1681 cgcactacct ttccaggacc gatgcggcct acggcgccga agacgaatcc gaggtcatcc
     1741 gatccgtcat ctcgccgccc cttcgctcgg aggcggaccg ccaggcgctg tggctcggcc
     1801 tggcgcacgc cgggctggac gtcgtcgcct cggaccacgt gccggaccgg ctggccgtcg
     1861 agaaggccgt ccccgcgccg cccttcccgc agatcagcaa cggcgcgccg ggaatcgaga
     1921 cgctcctggc ggtcacctac tcggcagggc cgggcgccgg gcgcatatcc ctggagcgca
     1981 tggtggacgt gctctccacg acgccggcgc gcctcttcgg cctggaggcc aagggcaccg
     2041 tcgaggccgg caaggacgcc gacctggtgc tgttcgaccc gacctcggcg cgcacggtca
     2101 cgcaggagag cctgcaccac accagcg
//
LOCUS       DATACA010000228         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_672101, whole genome shotgun sequence.
ACCESSION   DATACA010000228 DATACA010000000
VERSION     DATACA010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672101"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VHK28_04720"
     CDS             complement(<1..955)
                     /locus_tag="VHK28_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2141565.1"
                     /translation="MKTLTLLALVGLGAQLVDGSLGMAYGVTSTTLLLAIGTNPAAAS
                     ASVHLAEIGTTLASGVAHWRFGNVDWKVVAKIGVPGAIGAFLGATVLSSLSTEVAAPV
                     MALILLALGIYLLVRFTLRGVDRRNLGKPMRKRFLGPLGLVAGFVDTTGGGGWGPVGT
                     PAILASGRMEPRKVIGSIDTSEFLVALAASLGFLFALGSRGIDAAWVLALLIGGLIAA
                     PIAAWLVRHVPPRMLGSLVGGIIVLTNSRTLMRSDWIDASDTTRYAFYAVIYVVWAAA
                     VVHSYREYRKDRALESADALAAHAEGHAVVVEGDVVDDRVPV"
     gene            complement(1193..2194)
                     /locus_tag="VHK28_04725"
     CDS             complement(1193..2194)
                     /locus_tag="VHK28_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX2141566.1"
                     /translation="MDAAFVALVVIILVALAFDYTNGFHDAANAIAVAVSTKALTPRI
                     ALALAAVMNLIGALLSTEVAQTVGSGIIEPPQGSEGLEIVFAALIGAIVWNLITWWFG
                     LPSSSSHALIGGLVGSALAAAHTVQWDGVLNKVIVPMIVSPLVGFGLGYLFMLAILWS
                     FRRSHVAKANRGFRYAQIASSATMALGHGMQDAQKTMGIMTLALFTAGEIDSFEVPFW
                     VILSAALAISAGTYAGGFRIMRTLGRRIIQLTPAGGFSAQTVASSVMVTTATVFAVPV
                     STTHITTTSIMGVGATRRLSAVRWGVAGNIVAAWVVTLPAAGAVAAATYFVTDLIVG"
     gene            complement(2202..>2414)
                     /locus_tag="VHK28_04730"
     CDS             complement(2202..>2414)
                     /locus_tag="VHK28_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2141567.1"
                     /translation="KDLSDYWIEVNRIENEADKLYRRLLSRLYSGEFDALEILKLKEV
                     ADQLEEAADHFEHVANVVETIAVKES"
BASE COUNT          403 a          828 c          873 g          310 t
ORIGIN      
        1 ccaccggaac ccggtcgtcg acgacgtcgc cctccacgac cacggcgtgc ccctcagcgt
       61 gggcagcgag ggcgtccgcc gactcgagcg cccgatcctt gcggtactca cggtacgagt
      121 ggacgacggc ggcggcccac acgacgtaga tcaccgcgta gaaggcgtat cgggtcgtgt
      181 cggatgcgtc gatccagtcg ctgcgcatca gcgtccggct gttggtcagg acgatgatcc
      241 cgccgaccag cgagccgagc atccgcggcg ggacgtgccg gaccagccag gcggcgatcg
      301 gcgccgcgat cagcccgccg atcagcaggg ccagcaccca cgcggcgtcg atgccccgcg
      361 agcccagggc gaacaggaag ccgaggctcg cggcgagggc caccaggaac tcggaggtgt
      421 cgatcgagcc gatgaccttg cggggctcca tgcggccgct ggccaggatc gccggagtgc
      481 cgaccggccc ccagccaccg ccgccggtgg tgtccacgaa gccggcgacc aggcccagcg
      541 ggccgaggaa gcgcttgcgc atcggcttgc ccaggttccg ccggtccacg ccgcggaggg
      601 tgaagcggac caggaggtag atgcccagcg cgaggaggat cagcgccatc accggcgcgg
      661 cgacctcggt cgagaggctg gacaggacgg tcgcgccgag gaaggcgccg atcgcccccg
      721 ggacgccgat cttggcgacg accttccagt cgacgttgcc gaaccgccag tgggcgacgc
      781 ccgaggcgag cgtggtgccg atctcggcga ggtggacgga ggccgacgcg gccgccgggt
      841 tggtgccgat ggcgagcagc agggtggtgg aggtgacgcc gtaggccatg ccgaggctgc
      901 cgtccaccag ttgcgcaccc aggcccacca gtgccaggag ggtgagggtc ttcatgggct
      961 gggactccga acgtggaggg cgcggaacgc gtccggggac gcggtcgtcc tgcgcgtggt
     1021 cagggaggag gagtcagcag cccggacaac gcgcggtgca cacgcgcagc agatccacgt
     1081 ggatccgctg caccagcacc accgccgtct ctgccacggc gttatgtcta ccagatcgat
     1141 ggggatagcc cacaactccc cagacgaggg gggtgcggcg cgcccgggac cctcagccga
     1201 cgatgaggtc ggtgacgaag taggtcgcgg ccgccacggc gccggcggcc ggcagggtga
     1261 cgacccaggc ggccacgatg ttgccggcca ctccccagcg cacggccgac agccggcggg
     1321 tcgccccgac gcccatgatc gacgtggtgg tgatgtgcgt cgtcgacacc gggacggcga
     1381 acaccgtcgc ggtcgtcacc atgacgctgg aggccaccgt ctgggcggag aacccgccgg
     1441 ccggggtgag ctggatgatc cgccgcccga gcgtgcgcat gatgcggaac ccgccggcgt
     1501 aggtccccgc gctgatggcc agcgccgcgg agaggatgac ccagaacggg acctcgaagg
     1561 agtcgatctc gcccgccgtg aacagggcga gggtcatgat gcccatcgtc ttctgggcgt
     1621 cctgcatccc gtgcccgagg gccatcgtcg ccgagctcgc gatctgcgcg tagcggaagc
     1681 cgcggttggc cttggcgacg tgggaccgcc ggaaggacca caggatggcc agcatgaaca
     1741 ggtagcccag cccgaagccg accagcggcg agacgatcat cgggacgatc accttgttca
     1801 gcacgccgtc ccactgcacg gtgtgcgccg cggccagcgc cgacccgacc agcccgccga
     1861 tgagcgcgtg cgaggacgag gagggcaacc cgaaccacca ggtgatcagg ttccagacga
     1921 tcgcgccgat gagggcggcg aagacgatct ccagcccctc cgacccctgg ggcggttcga
     1981 tgatccccga cccgaccgtc tgggccacct cggtggacag cagcgccccg atcaggttca
     2041 tcaccgccgc cagcgcgagg gcgatccgcg gggtcagcgc cttggtcgac accgccaccg
     2101 cgatcgcgtt ggccgcgtcg tggaagccgt tggtgtagtc gaaggccagc gccaccagga
     2161 tgatgacgac gagcgccacg aacgccgcgt ccatgcgggg ctcaggactc cttgaccgcg
     2221 atcgtctcga ccacgttggc cacgtgctcg aagtggtcgg cggcctcctc cagctggtcg
     2281 gccacctcct tgagcttgag gatctccagc gcatcgaact cgccgctgta gagccgggag
     2341 agcagccggc ggtagagctt gtcggcctcg ttctcgatcc ggttgacctc gatccagtag
     2401 tcggagaggt cctt
//
LOCUS       DATACA010000229         3876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1957745, whole genome shotgun sequence.
ACCESSION   DATACA010000229 DATACA010000000
VERSION     DATACA010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957745"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VHK28_04735"
     CDS             complement(<1..767)
                     /locus_tag="VHK28_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2141568.1"
                     /translation="MPELRAWRVTDRETWTAFVESAPYRSFPQLWEWGELREPFGWAP
                     LRIAVGDNPQRPPIAVAQVLLRTVPALGWRLAYVPRGPVGHLDEAPVREALLAALRSL
                     ARTEGVATVKVDPEASPDSVLGAALLAPPWRAASRVQPPRTRIVDLAPSEDELLGALR
                     KKHRQYIHKAERAGVSVERLDGTADAATAQLALDDFYRIYVHTAERAGFVARAKRYYE
                     RVWGLFAPRGHARLAFAVHDGQRVATLFHFTCGDRAA"
     gene            complement(760..2292)
                     /locus_tag="VHK28_04740"
     CDS             complement(760..2292)
                     /locus_tag="VHK28_04740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX2141569.1"
                     /translation="MSVLDRRMGLGELADVIGPLRVVGLPVGEVTALAYDSRAVSPGT
                     LFFAVPGVHVDGHDFVPQAVERGALAVVVEREVPGTEVPQLVVERTRDALADAADAWY
                     NRPSQRLRVIGITGTDGKTTSAFLAVEVLLAGGRRPGLIGTVAVRVGDEMRPNLERNT
                     TPEAPELQALLAQMVAAGNDCVVMEATSHGLAQSRVRNARFQVGVFTNLTSEHLEFHG
                     SLDNYRSAKAMLFEEAPIAILNADDPSCGYFRERARERVVTYGVETDADLRATDVDAR
                     GDGTTFHLAAPDWSGNVRVPIPGSFNVHNALAAMALGWIEGVPLEVAAGAIGRSAGVP
                     GRMETVDEGQPFTVVVDYAHTADSLGKVLRVLRPLAGGQLMAVFGSAGERDPTKRAPM
                     GRVAAELADIVVVTDEDPRLEDPRAINEAIADGARAAGARDGESLLVIDDRPAAIARA
                     VSLARPGDVILLAGKGHEQSIIYGAQKRWWDEKEVARRALRAAGYRRPDAGGSDAGGS
                     DA"
     gene            complement(2289..3386)
                     /locus_tag="VHK28_04745"
     CDS             complement(2289..3386)
                     /locus_tag="VHK28_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2141570.1"
                     /translation="MIRVRPATNPDREAWQAFLERTNAGDFLHDWSWGAVAAFDGQPQ
                     RRFMAEEDGTVVAIVAAQVRPLLGGRSFWYVPRGPVMDYTHPSAAERLRELALALREQ
                     ARPDRAVAIKLEPRLERGTPPAALFDQLRGLRRDPRTLQVGQTRLVELADDDTMLAGF
                     DKDTRYAVRRAEREGVTVTTTTDGGDLLAIDALHSLVMETQRRAGFPRPRLERYRTAW
                     RELAGAGHATIFQARHDGRLLAAGMLVIVGDRSYYLFAGSLREERGEPKRYASYALQW
                     AMLRHARDAGVSVHDLWGIAPEDAGPEHPWYGVGLFKKGFGGRVVVWAGTWDIVVDPT
                     LYRLRSMVGGARRAVRAIPRRLLRSTRSLGR"
     gene            complement(3383..>3876)
                     /locus_tag="VHK28_04750"
     CDS             complement(3383..>3876)
                     /locus_tag="VHK28_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141571.1"
                     /translation="RFMAEEDGTVVAIVAAQVRPLLGGRSFWYVPRGPVMDYTHPSAA
                     ERLRELALALSISSLLEVAALLWALRRRIESIEDGEVARSAARSAVAAGAAALLMLGG
                     LTLVQATLPAMLDHGLGRLVAVGALTSAGGAIYLVVASALRAPEIGQLRALLARRRGG
                     AAA"
BASE COUNT          538 a         1469 c         1372 g          497 t
ORIGIN      
        1 tcggcggcac ggtcgccgca ggtgaagtgg aagagggtgg ccacccgctg gccgtcgtgg
       61 acggcgaagg ccagccgggc gtggccgcgc ggagcgaaca ggccccagac acgctcgtag
      121 tagcgcttgg cgcgagccac gaagccggcc cgctcggcgg tgtgcacgta gatgcggtag
      181 aagtcgtcca gcgccagttg ggcggtggcg gcatccgcgg tcccgtccag gcgctcgacg
      241 ctcacgccgg cgcgctccgc cttgtggatg tactgacggt gcttcttgcg cagggcgccc
      301 agcagctcgt cctccgacgg cgccaggtcc acgatccgag tgcgcggcgg ctggacgcga
      361 gatgcggctc gccagggcgg ggccagcagc gcggccccca gcacggagtc gggcgacgcc
      421 tcggggtcga ccttgacggt ggccaccccc tcggtgcgcg ccagcgagcg cagggcggcc
      481 agcagcgcct cgcgcacggg tgcctcgtcc aggtggccga ccggcccgcg cggcacgtac
      541 gccagccgcc agccgagggc cggaaccgtc cgcagcagga cctgcgcgac ggcaatcggt
      601 ggacgctgcg ggttgtcccc caccgcgatg cgcagcggcg cccagccgaa cggctcgcgc
      661 agctcgcccc actcccacag ctgcgggaac gagcggtagg gcgcggactc gacgaaggcg
      721 gtccatgtct ctcgatcggt gacgcgccag gcgcgcagct caggcatcgg atcctccggc
      781 gtccgatcct ccggcgtccg ggcgccggta gccggcggcg cgcagggcgc ggcgtgccac
      841 ctccttctcg tcccaccagc gcttctgggc gccgtagatg atgctctgct catggccctt
      901 gccggccagc aggatcacgt cgccggggcg cgccagcgac acggcgcggg cgatggcggc
      961 cggacggtcg tcgatcacca gcaggctctc gccgtcgcgc gctccggcgg cgcgtgcacc
     1021 atcggcgatc gcctcgttga tggccctcgg atcctccagg cgggggtcct cgtcggtgac
     1081 caccacgatg tccgccagct cggcagccac ccgacccatc ggcgcgcgct tggtgggatc
     1141 gcgctctccg gccgagccga agacggccat cagctgcccg ccggccagtg ggcgcagcac
     1201 gcgcagcacc ttgcccagcg agtcggcggt gtgggcgtag tcgaccacca ccgtgaacgg
     1261 ctgcccctcg tcgaccgtct ccatgcgtcc cggcacgccc gcgctgcgcc cgatcgcccc
     1321 ggcagccacc tccagcggca cgccctcgat ccagcccagg gccatggccg ccagcgcgtt
     1381 gtgcacgttg aagctgcccg gaatcggtac ccgcacgttg ccggaccagt ccggcgcggc
     1441 caggtggaag gtggtcccat cgccgcgggc gtcgacgtcg gtggcacgca ggtcggcatc
     1501 ggtctcgacg ccgtaggtga ccacacgctc gcgtgcccgc tcgcggaagt agccgcagga
     1561 ggggtcgtcg gcgttcagga tggcgatcgg cgcctcctcg aagagcatgg ccttcgcgga
     1621 ccgatagttg tccagggagc cgtggaactc caggtgctcg ctggtcaggt tggtgaacac
     1681 ccccacctgg aagcgggcgt tgcgcaccct ggactgcgcc agcccatgtg acgtggcctc
     1741 catcaccacg cagtcgttgc cggcggccac catctgcgcc agcagcgcct gcagctccgg
     1801 cgcctccggg gtcgtgttgc gctcgaggtt gggccgcatc tcatcgccga cccgcacggc
     1861 cacggtcccg atcaggcctg gccgccggcc gccggccagg agcacctcca ccgccaggaa
     1921 ggcgctggtg gtcttgccgt cggtgccggt gatgccgatc acccgcagcc gctgggatgg
     1981 tcggttgtac caggcgtccg cggcgtcggc cagcgcgtcc cgcgtgcgct ccacgaccag
     2041 ctgcggcacc tccgtgccgg ggacctcgcg ttccacgaca accgccagcg caccgcgctc
     2101 cacggcctgt ggcacgaagt cgtggccatc cacgtgtacg ccgggcacgg cgaagaagag
     2161 cgtccccgga ctgaccgcgc gcgagtcgta ggccagcgcg gtcacctcgc caaccggcag
     2221 gccgaccacc cgcagtggac cgatgacgtc cgcgagctcg ccgagtccca tgcgccgatc
     2281 caggacgctc atcggcccag gctcctggtc gatcgcagga ggcgtcgcgg gatggcgcgc
     2341 accgcccggc gcgcgccgcc gaccatggac cgcagccggt acagcgtggg gtcgacgacg
     2401 atgtcccacg tcccggccca gaccaccact cgcccgccga agcccttctt gaagaggccc
     2461 acgccgtacc acgggtgctc cggcccggca tcttccggtg cgatgcccca caggtcgtgc
     2521 acgctgaccc ccgcgtcccg agcgtgacgc agcatggccc actgcagggc gtagctcgcg
     2581 tagcgcttcg gctcgccacg ttcctcgcgc agcgacccgg cgaacaggta gtaggagcgg
     2641 tcgccgacga tcaccagcat gccggccgcc agcagccggc catcgtggcg ggcctggaag
     2701 atggtggcgt ggccggcacc ggccagctct cgccaggcgg tgcggtagcg ctccagccga
     2761 ggcctcggga agccggcacg gcgctgcgtc tccatcacca ggctgtggag ggcgtcgatg
     2821 gccagcaggt cgccgccgtc ggtggtcgtt gtgaccgtca cgccctcgcg ctcggcacgg
     2881 cgcaccgcgt aacgggtgtc cttgtcgaag ccggccagca tcgtgtcgtc gtcggcgagc
     2941 tcgaccagcc gggtctgccc cacctgcagc gtgcggggat cccggcgcag gccgcgcagc
     3001 tggtcgaaga gcgcggccgg cggcgtaccc cgctcgaggc gtggctcgag cttgatcgcc
     3061 accgcgcgat cgggacgcgc ctgctcgcgc aacgccaggg ccagctcgcg caaccgttcc
     3121 gcggccgacg ggtgcgtgta gtccatcaca gggccccggg gcacgtacca gaacgagcgg
     3181 ccgccgagca gcggccgcac ctgggcggcc acgatggcca ccaccgtccc gtcctcctcc
     3241 gccatgaagc gccgctgcgg ctgaccgtcg aaggcggcca ccgcgcccca gctccagtcg
     3301 tgcaggaagt cgccggcatt ggtgcgctcc aggaatgcct gccacgcttc tcggtcggga
     3361 ttggtggcgg gacggacgcg aatcatgccg ccgctccccc acgtcgccgg gcgagcagcg
     3421 cccgcagctg tccgatctcc ggggcgcgca gggcggaggc caccaccagg tagattgcgc
     3481 cgccggcgga ggtcagcgcc cccaccgcca ccagccggcc cagcccgtgg tccagcatcg
     3541 cgggaagcgt ggcctgcacc agggtcagcc cgccaagcat caggagcgcc gctgcgcctg
     3601 ctgccaccgc ggagcgcgcc gcggagcgtg cgacttcgcc gtcctcgatc gactcgatcc
     3661 ggcgccgcag cgcccacagc agggcggcca cctccaggag ggagctgatg gagagcgcca
     3721 gggccagctc gcgcaagcgt tccgcggccg acgggtgcgt gtagtccatc acagggcccc
     3781 ggggcacgta ccagaacgag cggccgccga gcagcggccg cacctgggcg gccacgatgg
     3841 ccaccaccgt cccgtcctcc tccgccatga agcgcc
//
LOCUS       DATACA010000230         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1475478, whole genome shotgun sequence.
ACCESSION   DATACA010000230 DATACA010000000
VERSION     DATACA010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475478"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VHK28_04755"
     CDS             complement(<1..366)
                     /locus_tag="VHK28_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141572.1"
                     /translation="MKIGEWETLGRARGLLQRQLGELGADELASHVTLDPDTGRLRIY
                     VATDVGLLEYRYGPAGQDPEGAWMLRGQLYRWPSVRGLRLQTDAQLDEDSGEVRSIWR
                     LVAEDPKMELNADSTGTDER"
     gene            602..>1797
                     /gene="gatB"
                     /locus_tag="VHK28_04760"
     CDS             602..>1797
                     /gene="gatB"
                     /locus_tag="VHK28_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX2141573.1"
                     /translation="MAVETRYETIIGIEVHAQLRTNSKMFCPCPTAPPPGQPDEPNAR
                     VCPICLVMPGTLPSINRRAVELVILTGLALGCEIQTDAVRFERKNYFYPDLPKGYQIS
                     QYALPLSANGQLLVPVPSEGTDVMVGITRAHLEEDTARLLHGGRGHTLVDFNRAGVPL
                     MEIVTEPDIRSPAQARAYGETLRDILRYIGASDGDMETGSMRIEGNVSLRPMGTERFG
                     TKVEVKNLNSFRSLERAMEFEVARHAEALEKGERLVQETRGWDESAGRTISQRSKEEA
                     NDYRYFPEPDLPPLRPSAAWVEELRTALPELPAARRGRYVDQLSLSAYDAGVLTADVA
                     LADYFDALVGAGVSAKAAANWVTGEFSRLLNQHAADGLRADAVALRPEGLAELISEVE
                     AGRVSHT"
BASE COUNT          287 a          648 c          608 g          254 t
ORIGIN      
        1 ccgctcatcg gttcccgttg agtcagcgtt gagctccatc ttggggtcct ccgccaccag
       61 tcgccagatg gaccgcacct cgccgctgtc ctcgtcgagc tgcgcatcgg tctgcaggcg
      121 aagcccgcga acgctgggcc accggtagag ctggccgcgc agcatccagg cgccttccgg
      181 gtcctggccg gccggtccgt atcggtactc gagcaggccc acgtcggtcg cgacgtagat
      241 ccgcagccgc ccggtgtccg ggtccagggt gacgtgcgag gcgagttcgt ccgctcccag
      301 ctcgccaagc tggcgctgta gcagcccgcg cgcccggccc agggtctccc attctccgat
      361 tttcattggc gcgcagggta gcaggcacgc gcgcccagac gcacaggcgc acggccgcac
      421 aatcgacccc tgatggcgca cgggtgcgcc cggcaggccc gggtggaagg gcctggcgct
      481 gtcagacccc atctgtgcag cacagccgca cggccgcgca gccgcacagc cgacccctgg
      541 tggcgcgcaa ctcctggccc agcccccgcc cacgctcccg ccactctcct agaatcgacg
      601 catggcggtc gaaacgcgct acgagacgat catcgggatc gaggtccacg cgcagttgcg
      661 cacaaactcc aagatgttct gcccgtgccc gaccgctccg ccgcctgggc aaccggacga
      721 gccgaacgcc cgagtctgtc ccatctgtct ggtcatgccc ggaacgctgc cgtccatcaa
      781 tcgccgcgca gtcgagctgg tgatcctgac cggcctggcg ctgggctgcg agatccagac
      841 cgacgcggtc cgcttcgagc gcaagaacta cttctacccg gacctgccca agggctacca
      901 gatcagccag tacgccctgc cgctgtcggc gaatgggcag ctgctggtgc ccgtcccctc
      961 tgaagggacc gatgtcatgg tcggcatcac ccgcgcccac ctcgaggagg acacggcgcg
     1021 cctgctgcac ggcggtcgag gccacacgct ggtggacttc aaccgagcgg gagtgccgct
     1081 gatggagatc gtgaccgagc ctgatattcg ctcgcctgcc caggcgcggg cctacgggga
     1141 gacgctgcgc gacatcctgc gttacatcgg tgcatcggac ggtgacatgg agaccgggtc
     1201 catgcgcatc gagggcaacg tcagcctgag accgatgggc accgagcgct tcggcacaaa
     1261 ggtcgaggtc aagaacctca acagcttccg gtcactggag cgcgccatgg agttcgaggt
     1321 cgcccggcac gccgaggcgc tggagaaggg ggagcggctg gtccaggaaa cgcgcggctg
     1381 ggacgagagc gccgggcgca ccatcagcca gcggagcaag gaagaggcga acgactaccg
     1441 ctacttcccg gagccggacc tgccgccgct gcgcccgtcc gcggcctggg tggaggagct
     1501 gcggaccgcg ctgccggagc tgcccgccgc ccgccgcggc cggtacgtgg accagctcag
     1561 cctgtccgca tacgacgcag gcgtgctgac cgccgacgtc gcgctggccg actacttcga
     1621 cgcgctggtc ggcgccggcg tgagcgccaa ggccgccgcc aactgggtga ccggcgagtt
     1681 cagccggctg ctgaaccagc atgccgccga tggcctgcgc gccgatgccg tcgcgctgcg
     1741 gccggagggc ctggccgagc tcatcagcga ggtagaggcc gggcgcgtct cgcatac
//
LOCUS       DATACA010000231         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1636433, whole genome shotgun sequence.
ACCESSION   DATACA010000231 DATACA010000000
VERSION     DATACA010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636433"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VHK28_04765"
     CDS             <1..259
                     /locus_tag="VHK28_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX2141574.1"
                     /translation="IYSYERPPTELPDEMLARMRADAAAWDRWEAEQPSFRRTASHWI
                     TSAKKAETRERRFNELLDALRNGTRPRPFLVERAAREPAGR"
     gene            320..1870
                     /gene="aceB"
                     /locus_tag="VHK28_04770"
     CDS             320..1870
                     /gene="aceB"
                     /locus_tag="VHK28_04770"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX2141575.1"
                     /translation="MADIGVAGVRVTDTSVPHADEVLTHEALGFVAQLHRSFNVRRLR
                     LLSDRGDRQARFDAGELPDFLPNTLHVREDPDWRVAPAPADLNDRRVEITGPAEPKMM
                     INALNSGARVFMADFEDALSPTWYNVVNGQWAVREAVRRTLAFETDEKAYALNDSGLA
                     TLLIRPRGWHLDEAHVLVDGTPVSASLFDFGMTFFHNAREQLERGSGPYFYLPKLESH
                     LEAALWNDVFVAAQAALDVPRGSVRATVLIETILAAFEMEEILYELRDHAAGLNAGRW
                     DYIFSVIKKLRSRPEFVLPDRAQVTMAVPFMRGYQQLLVRTCHRRGAHAIGGMSAFIP
                     NRREPEVTERALAAVREDKRREAGDGSDGTWVAHPDLVPVAREVFDAVLSGQPNQKGR
                     WPDVTVTAQDLLEVRIDGAAITEAGVQTNVSVALQYLASWLAGNGAAAINNLMEDAAT
                     AEISRSQLWQWRVHAARLANGRPMSAELYAAVRTEEVARLSAAFPDFRWTDAAALLDD
                     LVLADDFA"
BASE COUNT          324 a          728 c          685 g          330 t
ORIGIN      
        1 gatctactcg tacgagcggc cgcctaccga actcccggac gagatgctcg cccgcatgcg
       61 agcggacgct gcagcatggg accgatggga ggcggagcag ccctcgttca ggaggacggc
      121 gagccactgg ataacctcgg ccaagaaggc agagacccgg gagcggcgct tcaacgagct
      181 cctggatgcc cttcggaacg gcacccggcc gcggcccttc ctggtagagc gggcggcccg
      241 cgaacccgct ggccgctagg ccgccgggtc tgccacaatt tcctcgtgat cgttcacccg
      301 taccagtatc cggtggctcg tggctgacat cggcgtggcc ggggtccgcg tcacggacac
      361 atcggtccct cacgcggacg aggtcctgac gcacgaggca ctgggcttcg tggcgcagct
      421 ccatcggtcc ttcaacgtgc gacgcctgcg cctgctttcc gaccgcgggg accgccaggc
      481 gcgcttcgat gccggcgagc tgccggactt tttgcccaac acgctccacg tccgcgagga
      541 ccccgactgg cgggtggcgc ccgccccagc cgacctgaac gaccggcggg tcgagatcac
      601 cggccccgcc gagcccaaga tgatgatcaa cgccctgaac tcgggcgcgc gggtcttcat
      661 ggccgacttc gaggacgcgc tgtcccccac ttggtacaac gtggtcaacg gccagtgggc
      721 cgtgcgcgag gcggtgcgcc gaacgctggc cttcgagacc gatgagaagg cctacgcgct
      781 gaacgactcc ggcctggcca cgctcctcat ccgtccgcgc ggctggcacc tggacgaggc
      841 gcacgtcctg gtggatggga cgccggtcag cgcctcgctg ttcgacttcg ggatgacgtt
      901 cttccacaac gctcgggagc agctggagcg ggggtcgggt ccctacttct acctgcccaa
      961 gctggagtca cacctcgagg ccgcgctgtg gaacgacgtc ttcgtggccg cccaggccgc
     1021 cctggacgtt ccgcgcggat ccgtgcgcgc cacagtgctg atcgagacca tcctggccgc
     1081 cttcgagatg gaggagatcc tctacgagct gcgcgaccac gccgctgggc tcaacgccgg
     1141 ccgctgggac tacatcttca gcgtcatcaa aaagctgcgc tcccgccccg agttcgtgct
     1201 cccggatcgg gcccaggtca ccatggccgt gcccttcatg cgcggctacc agcagctgct
     1261 ggtacggacc tgccaccggc ggggcgcgca cgccatcggc ggcatgagcg ccttcattcc
     1321 caaccggcgc gagccggagg tgaccgagcg agccctggcc gccgtccgcg aggacaagag
     1381 acgcgaggct ggcgacgggt cggacgggac ctgggtcgca cacccggacc tggtgccggt
     1441 ggcccgcgag gtgttcgatg cggtcctgag tgggcagccc aaccagaagg gccgctggcc
     1501 ggacgtgacc gtcaccgcac aggatctgct cgaggtgcgc atcgacggcg ccgcgatcac
     1561 cgaggcgggc gtgcagacca acgtgagcgt ggctctgcaa tacctcgcct cctggctggc
     1621 aggcaacggg gcggcagcca tcaacaacct gatggaagac gccgcgaccg ccgagatcag
     1681 ccgctcgcag ctctggcagt ggcgggtgca cgccgctcgg ttggcgaacg gtagaccgat
     1741 gagcgccgag ctgtacgccg ccgtccgcac ggaggaggtc gcccgcctgt cggccgcctt
     1801 cccggacttc cgctggaccg atgccgccgc cctgcttgac gacctggtcc ttgccgacga
     1861 cttcgcctag ttcctcaccg tgcccgctta cccgcttctc ggctgaaaca ctggacgtcg
     1921 acgtcgcgct gtggtacgtt tgttcggtac aaacgcccgg tcgccgggca tcattgacgc
     1981 ggcaacggct cctctcactg gcttttcggc gagatcggat ttcgctgaag ccgcacgaga
     2041 ggagtctctt tctttgttca cctatac
//
LOCUS       DATACA010000232         3957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_431929, whole genome shotgun sequence.
ACCESSION   DATACA010000232 DATACA010000000
VERSION     DATACA010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431929"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHK28_04775"
     CDS             <1..208
                     /locus_tag="VHK28_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141576.1"
                     /translation="RRAWLGVGRDLVGQRVEAVVERHRWAQAGGRAELDLFFAEGRRV
                     DEQLRAAAEERAEERGATLQVVVA"
     gene            232..1284
                     /locus_tag="VHK28_04780"
     CDS             232..1284
                     /locus_tag="VHK28_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141577.1"
                     /translation="MTRLLALVWPDLRGPEAATSAFEPMLDALDDLSPRVEAVDAGVA
                     LVDITGLGALLDSERRIAARAVALVRGVAPLPVRCGVGDNRWLAVLAARLARPERPDA
                     PAAFRVLERAELAALSLDLLPADAATRQRFALFGLTSMGQLASLPRSAVGAQFGAIGE
                     RLQGLARGEDGRPLAPRRRPERLVRRVPFEPALDGVGATALALRAACAELCDALRARH
                     LAPGRARLVLVLEDAPRLAVTIAFPAPALEPDWIARLLIGRLEAAARDRRMEEEPRVA
                     ALTLAFDRLADPAARQLPAFEAQAGRWEELRWSLERLVARFGEGRLWRAEHDRPTASL
                     ADRQWRLVDIGPEAGS"
     gene            1281..1535
                     /locus_tag="VHK28_04785"
     CDS             1281..1535
                     /locus_tag="VHK28_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141578.1"
                     /translation="MTRLVAGHPLVQVETDADGNPVRLRLDGVTHAEVGICNRWRLDD
                     DWWRTPVARAYFKLVTRDGLLCTVYLDELRGTWHLERVFD"
     gene            1699..2118
                     /locus_tag="VHK28_04790"
     CDS             1699..2118
                     /locus_tag="VHK28_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141579.1"
                     /translation="MSDFNRQPGAIDDPGMKGGIDPDVDELPPGDTAGNTGSEGEPQA
                     AELDLPGPEDPVAWSYIEPGTTVVGREGVKVGTVQAMLGTEAEGIFHGIAIDPSSGGP
                     PRVIPADAVTSLTPNEVQVQVATDAVESLEEYHGEQG"
     gene            2151..2918
                     /locus_tag="VHK28_04795"
     CDS             2151..2918
                     /locus_tag="VHK28_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX2141580.1"
                     /translation="MSVIDRLWPQPAAGIPLDEAFADLSLPASPNGRPIVATNMVTSI
                     DGRAQLSGTAEGLGSREDRRLMRLYRAGFDAVGSGVGTLRADDFYSRLPADLAQRRSA
                     AGRPPQPVAILIGGTSAIPTDRRWFTYQDQPRIVVVGADSPHATDQPLPGVETWVAPT
                     AVPQPAWVLERLAARGVRSLLLEGGPTTNSAFLAARALDELYWTIGPRIVARDALRMI
                     ASLDEGEMPKPVEATLVSVHRSGDELFVRYRLGSIGP"
     gene            2915..3817
                     /locus_tag="VHK28_04800"
     CDS             2915..3817
                     /locus_tag="VHK28_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2141581.1"
                     /translation="MTLNLGRLGAWGHLDSLDALQAREYARRVEELGFGTLWVPETVG
                     REPFTLLGMLAGETSSLLLGTSVVSIWGHDAQTTRMAALTLQEATGGRFVLGVGVSHP
                     HLAQRLRGHTFDRPLTRTREFLAAYRSAIYKGPMSPGAPDPPVLVAALRERMLALAAT
                     EADGAFPYLVTPERVAWTRSVLDTAAGTGPCPVLAVSMPAVVETDADAAREIARGYLA
                     PYLRTPTYQASWLAQGFGEADWAKPGSYALVDGMVAWGDAETLQARIADLVAAGADHV
                     AVIPLGPDGSTENIDVLEALAAVG"
BASE COUNT          480 a         1434 c         1490 g          553 t
ORIGIN      
        1 acggcgcgcc tggctgggcg tggggcgcga cctggtgggg cagcgggtcg aggccgtggt
       61 ggaacgccac cgatgggcgc aggccggcgg gcgtgcggag ctggacctgt ttttcgccga
      121 ggggcgtcgc gtggacgagc agctgcgtgc cgcagccgag gagcgggcgg aggagcgggg
      181 cgccacgctc caggtggtgg ttgcatgagc cgcgggtttg caacaccggc catgacccgg
      241 ctcctcgcgc tcgtctggcc ggacctgcgc ggaccggagg cagccaccag cgccttcgag
      301 ccgatgctcg acgcgctgga cgacctctcc ccgagggtcg aggcggtgga cgccggcgtg
      361 gccctggtcg acatcacggg cctcggggcg ctgctggaca gcgagcgccg gatcgcggca
      421 cgtgccgtgg cgctggtccg gggagtggcg ccgctccccg tgcgctgcgg ggtgggggac
      481 aaccgctggc tggcggtgct ggccgcgcgg ctggcgcgcc cggagcgccc cgacgccccg
      541 gccgccttcc gcgtcctgga gcgtgcggag ctggccgccc tgtcgctgga cctgcttccc
      601 gcggacgcgg ccacgcgtca gcggttcgcg ctgttcgggc tgacgagcat gggccagctg
      661 gcgtcgctgc cacgctccgc ggtcggtgcc cagttcggcg ccatcggaga gcggctccag
      721 ggattggccc gcggcgagga tgggcggccg ctcgccccgc gccggcgtcc ggaacgcctg
      781 gtgcgtcgcg tgccctttga gccggcgctg gacggggtgg gagccaccgc gctcgccctg
      841 cgagccgcct gcgccgagct gtgcgacgcg ctgcgtgccc gccacctggc gcctggccgc
      901 gcccggctgg tgctggtgct ggaggacgcg ccgcggctgg cggtgaccat cgccttcccg
      961 gcgccggcgc tggagccgga ctggatcgcg cgcctgctca tcggtcgcct ggaggccgcg
     1021 gcgcgcgacc gccgcatgga ggaggagcca cgcgtggcgg cgctgaccct cgccttcgac
     1081 cggctggccg acccggccgc gcggcagcta ccggccttcg aggcacaggc cggccgctgg
     1141 gaggagctgc gctggtcact ggagcggctg gtcgcccgct tcggtgaggg ccgcctgtgg
     1201 cgggccgagc acgaccggcc gaccgcgtcg ctggccgatc ggcagtggcg cctggtggac
     1261 atcggtccgg aggcgggctc gtgacccgcc tggttgccgg tcacccgctg gtgcaggtgg
     1321 agaccgatgc cgacggcaat cccgtgcgtc tgcgccttga cggcgtgacc catgccgagg
     1381 tcggcatctg caaccgatgg cggctggacg acgactggtg gcggaccccg gtggcccgtg
     1441 cctacttcaa gctggtgact cgtgatgggc tgttgtgcac cgtctacctg gacgagctgc
     1501 ggggcacctg gcacctggag cgcgtcttcg actgatccct ggcgcgtctg atgcccgagg
     1561 gtggcgtgct gtcaggcccg tttgtgcgcc tgtgcggctg tgcgcctgtg ttgcacgaag
     1621 cggcatgacg gcggcaccgg ggcctgcgcg ccacccgcga gcgggcatgg cacccgacgt
     1681 gcaacccctc gcaaccccat gagcgatttc aaccgccagc ccggggccat cgacgatccc
     1741 ggcatgaagg gcggcataga cccggacgtc gacgagctgc cgcccggcga cacggcgggg
     1801 aacaccggga gcgagggtga gccgcaggca gcggagctgg acctgcccgg cccggaggac
     1861 ccggtggcct ggagctacat cgagcccggc acgaccgtgg tcgggcgcga gggcgtcaag
     1921 gtcgggaccg tgcaggccat gctggggacc gaggcggagg ggatcttcca cggcatcgcg
     1981 atcgacccat cgtccggcgg accaccgcgg gtgatcccgg ccgacgcggt cacctccctc
     2041 accccgaacg aggtgcaggt gcaggtcgcc accgacgccg tcgagtccct cgaggagtac
     2101 cacggcgagc agggctgacc gcccgccgag cgcccgcgta ccatgcggcc gtgagcgtga
     2161 tcgatcgcct gtggccgcag ccggccgccg gcatcccgct cgacgaggcc ttcgccgacc
     2221 tttcgctgcc ggcttccccc aacgggcgcc cgatcgtggc caccaacatg gtcaccagca
     2281 tcgacggtcg tgcgcagctg tccggcaccg ccgaggggct gggctcgcgc gaggatcggc
     2341 gcctgatgcg cctctaccgg gccggcttcg acgccgtggg gagcggggtc ggcaccctgc
     2401 gcgccgacga tttctactcg cggctgccgg cggacctggc gcagcggcgg agcgcggccg
     2461 gccggccacc gcagccggtc gccatcctca tcggcggcac gtccgccatc cccaccgacc
     2521 gccgctggtt cacctaccag gaccagccgc gcatcgtggt ggtgggtgcc gacagcccgc
     2581 atgcgaccga ccagccgctc cccggggtgg agacctgggt cgcgccgacc gcggtgcccc
     2641 agccggcctg ggtgctggag cgtctcgcgg cacgcggcgt acgctcgctg ctcctcgagg
     2701 gaggcccgac caccaactcc gccttcctgg cggcacgggc cctggacgag ctgtactgga
     2761 ccatcggtcc gcggatcgtg gcgcgcgatg ccctgcgcat gatcgcctcc ctggacgagg
     2821 gggagatgcc aaagccggtg gaggccacgc tggtatcggt ccatcggtca ggggacgagc
     2881 tgttcgtgcg ctaccgcctg ggtagcatcg gtccatgacg ctcaacctgg ggaggctggg
     2941 tgcgtggggg cacctggact cgctggatgc cctgcaggcg cgcgagtacg cgcgacgggt
     3001 cgaggagctc ggcttcggca ccctgtgggt gcccgagacg gtcgggcgcg agccgttcac
     3061 cctgctcggc atgctggccg gtgagaccag cagcctgctg ctgggcacca gcgtcgtctc
     3121 catctggggc cacgacgcgc agaccacgcg catggcggcg ctcacccttc aggaggcgac
     3181 cggcgggcgg ttcgtgctgg gcgtgggcgt ctcgcacccg cacctggcgc aacggctgcg
     3241 cgggcacacc ttcgaccgcc ccctgacgcg cacgcgcgag ttcctggctg cctatcggtc
     3301 cgccatctac aagggaccga tgtctcccgg tgcgccggat ccccccgtcc tggtcgcggc
     3361 actgcgcgag cggatgctgg ccctggccgc cacggaggcg gatggtgcct tcccgtacct
     3421 ggtcaccccg gagcgggtgg cgtggacgcg gtccgtcctg gacacagccg ccggaaccgg
     3481 tccttgcccg gtgctggcgg tcagcatgcc cgccgtggtg gagaccgatg ccgacgccgc
     3541 ccgcgagatc gcccgcggct acctggcgcc ctacctgcgc acccccacct accaggcctc
     3601 gtggctggcc cagggcttcg gcgaggccga ctgggccaaa cccggcagct acgcgctggt
     3661 ggacggcatg gtggcctggg gagatgccga gacgctgcag gcgcgcatcg ccgacttggt
     3721 ggccgccggt gccgaccacg tggccgtcat cccgctcggc ccggacggct cgaccgagaa
     3781 catcgacgtg ctcgaggcac tcgccgcggt cggctgatcc ggctctatga tcgcacccat
     3841 gcccggcacc cccgagatcg attcgcgcct gcgccgccgc gcggtggcca cctacttcct
     3901 gaccgccgcg ctaagcagca ccgcgtacat ctccgccttc acggtggccg cgctggc
//
LOCUS       DATACA010000233         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2118902, whole genome shotgun sequence.
ACCESSION   DATACA010000233 DATACA010000000
VERSION     DATACA010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118902"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VHK28_04805"
     CDS             <1..723
                     /locus_tag="VHK28_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141582.1"
                     /translation="RNLLHIRRTPELLLDVTLSPIMFVLLFSFVFGGAIDVPGSYVNF
                     LMAGIFVQTIAFAGVYTAVLLANDLKNGMIDRFRSLPMAQSAVIVGRTFTDVLRGMLA
                     IGVMWIVGLIVGFRPEGGVLASLAAIGLMLVFGYALSWLGVAMGLVVRTPEALQGIMF
                     ATVFPLTFASSAFVPTDTMPGWLQVFAENQPMTLVVNATRSLLLEGSGGPDLIPALIW
                     TVGILAVAFPLAVWLYANRAVQ"
     gene            742..1197
                     /locus_tag="VHK28_04810"
     CDS             742..1197
                     /locus_tag="VHK28_04810"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2141583.1"
                     /translation="MDVYPWLKTVHVMLAIVAVGTNVTYGIWQARAGREPQHILWTLR
                     GVKFLDDRVANPSYIALAVVGVLLVLTGPWEFEMLWIAVSIGLYILLAVVGLFLYSPT
                     LSRQIGVYETDGPASPEFSALTGRSRLLGALMGVLVAVIVVLMVVKPGA"
     gene            1194..>1988
                     /locus_tag="VHK28_04815"
     CDS             1194..>1988
                     /locus_tag="VHK28_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2141584.1"
                     /translation="MSITASTDRRERMHRSVAPFLRFFNGPFASLNLEPDVANFAVGN
                     PQEMPLPDYVGALQAALPPQDKDWFAYKLSEPESQRTVAETLTARTGMSWDAADVHMT
                     NGGFAALAVTLRTLVEPGDEVVFLSPPWFFYEILVLAAGGEPVRVRLEPPAFDIEPER
                     ITAAIGPSTRAVILNSPHNPSGRVYTEADLRALADVLTDASGRLGHPIWLISDEPYNR
                     ILFDGRGYTSPSQVYPHTVITYSYGKTLLAPGQRIGYVTVPPSLPAD"
BASE COUNT          282 a          690 c          658 g          358 t
ORIGIN      
        1 cgcaacctgc tgcacatccg gcgcacgccc gagctgctgc tcgacgtgac cctgtcgccg
       61 atcatgttcg tgctgctctt cagcttcgtc ttcggcggcg cgatcgacgt gccggggtcg
      121 tacgtgaact tcctgatggc cggcatcttc gtgcagacca tcgccttcgc cggggtctac
      181 accgcggtcc tgctggccaa cgacctgaag aacggcatga tcgatcgctt ccgctcgctc
      241 ccaatggcgc agtccgcggt catcgtgggg cgcaccttca ccgacgtgct gcgcgggatg
      301 ctggccatcg gtgtcatgtg gatcgtgggg ctgatcgtcg gattccgccc ggagggtggg
      361 gtactggcat cactggcagc catcggtctc atgctggtct tcgggtatgc gctgtcctgg
      421 ctcggcgtgg ccatgggctt ggtggtgcgc accccggagg cgctgcaggg aatcatgttc
      481 gccaccgtct tccccctgac cttcgccagc agcgcgttcg tgccgaccga caccatgccc
      541 ggctggctgc aggtcttcgc cgagaaccag cccatgacgc tggtggtcaa cgccacccgc
      601 agcctgctgc tggagggaag cggaggcccg gacctcatcc cggcgctgat ctggacggtc
      661 ggaatcctgg ccgtggcctt ccccctagcc gtctggctct acgccaaccg cgccgtacag
      721 taagaaagga ggccacacgg catggacgtc tatccctggc tgaagaccgt gcacgtgatg
      781 ctcgccatcg tggcggtcgg gacgaacgtg acctacggca tctggcaggc gcgggctggc
      841 cgtgagccgc agcacatcct ctggacgctg cgcggggtca agttcctgga cgaccgggtg
      901 gcgaacccct cctacatcgc gctcgccgtg gtcggcgtgc tgctggtgct gaccgggccg
      961 tgggagttcg agatgctgtg gatcgcggtc tccatcggcc tgtacatcct gttggccgtg
     1021 gtcggcctgt tcctgtactc tccgaccctc tcgcgacaga tcggggtgta cgagaccgat
     1081 ggcccggcgt ccccggagtt ctcggcactg accggccgga gccgcctgct cggcgcgctg
     1141 atgggcgtgc tggtggccgt catcgtggtg ctcatggtgg tcaagccggg cgcatgagca
     1201 tcactgcctc caccgatcgg cgggaacgga tgcaccgcag cgtcgcgccc ttcctgcgct
     1261 tcttcaacgg accgttcgcc agcctcaacc tggagcccga cgtggcgaac ttcgcggtcg
     1321 gcaacccgca ggagatgccg ctgcccgatt acgtaggcgc cctgcaggcc gcgctcccgc
     1381 cgcaggacaa ggactggttc gcctacaagc tctccgagcc ggagtcgcag cgcaccgtgg
     1441 ccgagaccct gaccgcgcgg accggcatga gctgggacgc ggcggacgtg cacatgacca
     1501 acggcggctt cgcggcgctg gcggtgacgc tgcggacgct ggtcgagccc ggtgacgagg
     1561 tggtgttcct ctcgccaccg tggttcttct acgagatcct ggtgctggcg gcgggcggcg
     1621 agccggtgcg cgtacggctg gagccgcctg ccttcgacat cgagcctgag cggatcaccg
     1681 cggcaatcgg tccatccacc cgcgcggtta tcctcaacag cccgcacaac ccgagcgggc
     1741 gggtctacac ggaggcggac ctgcgcgcgc tggccgatgt cctgaccgat gcgtccgggc
     1801 gcctcggtca tcccatctgg ctcatttccg acgagcccta caacaggatc ctgttcgacg
     1861 gccgcggcta caccagcccg tcgcaggtct acccgcacac cgtgatcacc tactcgtacg
     1921 gcaagacgct gctggcgccc ggccagcgga tcggctacgt gaccgttccg cccagcctgc
     1981 cggccgat
//
LOCUS       DATACA010000234         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_673119, whole genome shotgun sequence.
ACCESSION   DATACA010000234 DATACA010000000
VERSION     DATACA010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673119"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VHK28_04820"
     CDS             <1..162
                     /locus_tag="VHK28_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2141585.1"
                     /translation="RRIASGVGVERTFMVHAPRIEKIEVVRHGVARRAQLYFLRNRVG
                     KSATLRERR"
     gene            167..913
                     /locus_tag="VHK28_04825"
     CDS             167..913
                     /locus_tag="VHK28_04825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX2141586.1"
                     /translation="MTDLLMGSVAAPRRRTRRHSKPRDAHMRHERELRLAGYRRIAGI
                     DEVGRGSLAGPVVAAAVILPERPRLKGLRDSKVLGRARREALYEQILDRAEAVGVGCV
                     EVEVIDRINILQATKLAMRQAIQRLPGAPDHLVIDALSLREVELPQRPIIDGDAICAS
                     IAAASIVAKVTRDRICAELDEVFPAYGFARNKGYGTRRHYDALMAEGPCEWHRRSFAP
                     IRMLVAGQQLSFDLLLDADLPTEEELDPPD"
     gene            961..1329
                     /locus_tag="VHK28_04830"
     CDS             961..1329
                     /locus_tag="VHK28_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX2141587.1"
                     /translation="MTDPRHDLGIRGESAVGAWLRQAGWQVLERRWRSPFGELDLVCL
                     DPDATLVGVEVKLRSTSRAGSGAESVDRRRLARLRRALGSYAATTNLRSAAVRLDLVT
                     LEPTADGRWRAVRTPAIDLW"
     gene            complement(1343..>2431)
                     /locus_tag="VHK28_04835"
     CDS             complement(1343..>2431)
                     /locus_tag="VHK28_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX2141588.1"
                     /translation="ATAPTAPAAPQPAAAPARTHRVARGETLTAIARRYGTSIGAIAA
                     ANGLSNPSFIRSGQALTIPAAPASATAPTAPAAPQPAAAPARTHRVARGETLTAIARR
                     YGTSIGAIAAANGLSNPSFIRSGQALTIPAAPASATAPTAPAAPQPAAAPARTHRVAR
                     GETLTWIARRYGTSIGAIAAANGLSNPSFIRTGQVLTIPGSAAAAAPAAPAAPAAQAA
                     PAGMPASMAALVSKRQAVRDLIVAEAGRQGVPAAFALAVAWQESGWQQGVVSSAGAIG
                     IMQIIPASGEWVGQAMLGERVNLHDAASNVRAGVRLLRHYLDRYGDRSLALAAYYQGQ
                     SAVDRHGIYSVSRPYIASILRLEQIFAR"
BASE COUNT          322 a          856 c          908 g          345 t
ORIGIN      
        1 cgccgcatcg ccagcggcgt cggcgtggag cgcaccttca tggtgcacgc accgcgcatc
       61 gagaagatcg aggtggtccg tcacggtgtc gcgcgtcgcg cccagctcta cttcctccgc
      121 aaccgggtcg gcaagtccgc caccctccgc gagcgccgct gatcgcatga ccgatctcct
      181 catgggatcg gtcgccgccc cccggcgccg cacgcgccgc cacagcaagc cgcgcgacgc
      241 ccacatgcgg cacgaacgtg agctgcgcct ggcgggctac cgacgcatcg ccggcatcga
      301 cgaggtcggc cgcgggtcgc tggccgggcc ggttgtcgcc gccgccgtca tcctccctga
      361 acggccgcgg ctgaagggcc tgcgcgacag caaggtgctg ggccgcgcgc ggcgcgaggc
      421 tctctacgag cagatcctgg atcgcgccga ggccgtcggc gtcggttgcg tggaggtcga
      481 ggtcatcgac cgcatcaaca tcctgcaggc gaccaaactg gccatgcgcc aggccatcca
      541 gcgtctgccg ggagcgccgg accacctggt catcgatgcg ctcagcctcc gcgaggtcga
      601 gctgccgcag cgaccgatca tcgacgggga cgccatctgc gcctcgatcg ccgcggccag
      661 catcgtggcc aaggtgaccc gtgaccgcat ctgcgccgaa ctggacgagg tgttcccggc
      721 ctacgggttc gcacgcaaca agggctacgg gacgcggcgc cactacgacg cactgatggc
      781 ggagggcccg tgtgagtggc accgacgctc cttcgccccg atccgcatgc tggtcgccgg
      841 ccagcagctc agcttcgacc tgctgctgga cgcggacctg cccaccgagg aggagctgga
      901 cccaccggac tgatcgggca ccagcgttcg tggtcgctgc tcgcctcccg ggaacgagcc
      961 atgaccgatc ccaggcacga cctggggatt cgcggcgagt cggcggtggg tgcctggctg
     1021 aggcaggcag gctggcaggt gctcgagcgt cgctggcggt ctccgttcgg cgagctcgac
     1081 ttggtgtgcc tcgacccgga cgcgacgctg gtcggggtgg aggtgaagct gcgcagcacg
     1141 agccgcgcgg gcagcggcgc ggagagcgtc gatcgtcgac ggctggcacg cctgcggagg
     1201 gcgctcggca gttacgcggc cacgaccaac ctccgttcag cggcggtgcg gctcgacctg
     1261 gtcaccctgg aacccaccgc cgacggcagg tggcgcgcag tccgcacacc cgccatcgac
     1321 ttgtggtgag cgccgtgcgc cgctacctgg cgaagatctg ctcgagccgc aagatggagg
     1381 cgatgtaggg ccgcgacacc gagtagatgc cgtgccgatc caccgcgctc tggccctggt
     1441 agtaggcggc cagcgccagg gagcggtccc cgtatcggtc caggtagtgc cgcagcaggc
     1501 gcacgccggc gcgcacgttg gatgcggcat cgtgcaggtt cacgcgctcg cccagcatgg
     1561 cctggccgac ccactcgccg ctggccggaa tgatctgcat gatgccgatg gcgcccgcgc
     1621 tggacaccac gccctgctgc cagccagact cctgccaggc gacggccagg gcgaaggcgg
     1681 cgggcacccc ctgccggccg gcctcggcca cgatgagatc gcgcaccgcc tgccgcttgc
     1741 tcaccagcgc tgccatggag gccggcatgc cggcgggcgc cgcctgcgcc gccggagcgg
     1801 ccggagccgc cggagccgcg gcagcagccg aaccggggat ggtcaggacc tggccggttc
     1861 gaatgaacga tggattgctc agcccgttgg cggcggcgat ggcaccgatg ctggtcccgt
     1921 agcgccgcgc gatccaggtc agcgtttcgc cgcgggccac gcggtgcgtg cgcgcgggtg
     1981 cggctgccgg ctgcggtgcc gccggtgcgg ttggagccgt ggctgacgcg ggcgccgcgg
     2041 ggatggtcag tgcctgcccg ctgcgaatga acgatggatt gctcagcccg ttggcggcgg
     2101 cgatggcgcc gatgctcgtc ccgtagcgcc gcgcgatggc cgtcagcgtt tcgccgcggg
     2161 ccacgcggtg cgtgcgcgcc ggtgcggctg ccggctgggg tgctgccggt gcggttggag
     2221 ccgtggctga cgcgggcgcc gcggggatgg tcagtgcctg cccgctgcga atgaacgatg
     2281 gattgctcag cccattggcg gcggcgatgg cgccgatgct cgtcccgtag cgccgcgcga
     2341 tggccgtcag cgtttcgccg cgggccacgc ggtgcgtgcg cgccggtgcg gctgccggct
     2401 ggggtgctgc cggtgcggtt ggagccgtgg c
//
LOCUS       DATACA010000235         5303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2521685, whole genome shotgun sequence.
ACCESSION   DATACA010000235 DATACA010000000
VERSION     DATACA010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521685"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1535
                     /gene="ffh"
                     /locus_tag="VHK28_04840"
     CDS             201..1535
                     /gene="ffh"
                     /locus_tag="VHK28_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX2141589.1"
                     /translation="MFESLSARLQAVTQRLRGKAKITEADLDVALREIRLALLEADVN
                     FRVVKDFVARVRERALGLEVLSGLNAGQQVVKVVHDELVATLGGETQPLRLDARPAVV
                     MLVGLQGSGKTTTAAKLAVRLRKDGRRPLLVAADVYRPAAVDQLVQLGDQTGVEVHTR
                     PTGTPALEVARSGLEVARQRGHDLVLVDTAGRLHVDEAMMDELVRIAGSITPSETLLV
                     VDAMTGQEAVKVAEAFHARLPVTGLVLTKMDGDARGGAALSIRSVTGLPISYIGTGER
                     TDGLEAFHPDRLAQRILGMGDILSLVERVQETVDQDEAERVAQRMMEARFTLEDFRSQ
                     LTQIKKMGPIGQLLEMVPGAGQLAGAAQQAVDEGQLKRVEAIIDSMTPEERRRPDLIK
                     ASRRRRIAMGSGTTTADVNRLLKQFTDMQKLMRQMSGGRMPNLGGLRGMLGR"
     gene            1537..3093
                     /locus_tag="VHK28_04845"
     CDS             1537..3093
                     /locus_tag="VHK28_04845"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEX2141590.1"
                     /translation="MQRLVIALTTLLTLTGAAVVTGYLLVFAVGSDRLAQATPAGTAA
                     YATVYLQPSTGQKMNLAQLLGRVPGFADAASLDQKIHEITARLMGEAGVDYERDVRPW
                     LGNQVAIAARPDAADPLAADTWFLAATTDAGAAEAALERIATDQGSQVTTSEHEGVTV
                     SAADGTAWAVLDELVIFAPDPASVTEALDVAAGRADSLASEAGFRDAMGRLPSDHLAA
                     VYLDSTEALASSEADTPATGYTDLSLALVAEAEGLHLFGVAPFDADEAPESMVDAFEL
                     GRQVGELTGWMPEDTQAALVVFGLSQSLTAAEEQLGSAPGGDAVADALNQVRAVAALG
                     LGIDIDDDVLPLFDGETAIAVGGIDTGTPGAQLLLRPSDAQAAEAALGRLRDALAGRG
                     AAVDEEQIDGTTVTTVEVPELGTVSYAVRDGVVAAGLTTDAVIGALDAGAGTSLADTE
                     RYADAWELAGTRGGNEGYLDVAAVVDGFGDEMGATGEVGDILREIGALAATMPARDDH
                     SEIHIVLTVR"
     gene            3142..3501
                     /gene="rpsP"
                     /locus_tag="VHK28_04850"
     CDS             3142..3501
                     /gene="rpsP"
                     /locus_tag="VHK28_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX2141591.1"
                     /translation="MAVRIRLARVGATKRPSYRVVAIDSRKPRDGRALEILGFYNPLT
                     DPATVQIDADKLNAWLSKGATPSETVVKLMRQAGIGGAAPETPAAAPARRKAASGGTA
                     ARSRSKKEPATAPAAEE"
     gene            3498..3737
                     /locus_tag="VHK28_04855"
     CDS             3498..3737
                     /locus_tag="VHK28_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX2141592.1"
                     /translation="MKDLLLYVARHLVDNADAVSVEVEEDEDEVTLVLTVEENDMGRV
                     IGRDGRIANAIRTLLRVMGARDGRHIELEIVSDQE"
     gene            3742..4221
                     /gene="rimM"
                     /locus_tag="VHK28_04860"
     CDS             3742..4221
                     /gene="rimM"
                     /locus_tag="VHK28_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX2141593.1"
                     /translation="MAVARVLGAKGLSGAMRVEPLSDVPGRLEVGSVLFIEGEDDPHR
                     VRLSEPAGRTPVIALEGIEDRYGVERLIGSYLEVEPEALPEGTYYWHQLVGLEVTDED
                     GTPLGTLVEVFRAGENEVYRIEGAGGELLLPSLREVVRGIDLESGTMIVRRQDSEEV"
     gene            4223..4948
                     /gene="trmD"
                     /locus_tag="VHK28_04865"
     CDS             4223..4948
                     /gene="trmD"
                     /locus_tag="VHK28_04865"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX2141594.1"
                     /translation="MRFDVVTLFPELFEVPLRTSVVGRALERGVVEVGVHDLREHGLG
                     RHRSVDDYPYGGGAGMVLRPEPLFAAVEPLRASGAHVILLDPAGQRLTDAVARELAQL
                     PHITLVCGRYEGVDERARELVHREVSIGDYVLTGGELPALVLIDAVARLVPGVIEEAS
                     SASDSFSEGLLEHPQYTRPEEFRGMEVPSVLLSGNHGEVDRWRRREALRRTLERRPDL
                     LDAAPLTGDDRRMLEELREELGE"
     gene            5097..>5303
                     /locus_tag="VHK28_04870"
     CDS             5097..>5303
                     /locus_tag="VHK28_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX2141595.1"
                     /translation="MDAIKELEREQLRTDVPQIATGDTVRVAVKVVEGNRERIQVFEG
                     TVIRMRGGGLNQQITVRRIASGVGV"
BASE COUNT          782 a         1806 c         1953 g          762 t
ORIGIN      
        1 ggggcgaaaa cggctacttc ctgcaggatc tgccgagata gatcggtcag gtgtcgcgcc
       61 gtccgtgcgt cgtgtgcgat ccctccccga tagatcgggc gtgtccgcta ggcttccgct
      121 gcctcgttgc tcccatctgt cctgacgcct agaattggca gccgccatct tccggctccc
      181 acccccgtat cggtccattc atgttcgaat ccctctccgc gcgccttcag gccgtcacgc
      241 agcggctgcg tggcaaagcc aagatcaccg aggccgacct cgacgtagcc ctccgcgaga
      301 tacgcctggc gctgctcgag gcagacgtca acttccgcgt cgtcaaggac ttcgtggccc
      361 gggtgcgcga gcgtgcgctg ggcctcgagg tgctcagcgg tctgaacgcc ggccagcagg
      421 tcgtgaaggt cgtccacgac gagctggtgg ccaccctggg aggcgaaacc cagccgctgc
      481 gtctggatgc gcggccggcc gtggtgatgc tggtcggcct gcagggatcg ggcaagacga
      541 cgaccgccgc caagctcgcg gtccggctgc gcaaggacgg ccggcgcccg ctgctggtgg
      601 ctgccgacgt ctaccgtccg gcggcggtcg atcagctggt gcagctcggc gaccagaccg
      661 gcgtcgaggt ccacacccgg cccaccggca cgccggccct ggaggtcgct cgctcggggc
      721 tggaggtggc tcggcagcgc ggccacgacc tcgttctggt ggacaccgcc ggccggctgc
      781 acgtcgacga ggcgatgatg gacgagctgg tgcgcatcgc cggctccatc accccgtccg
      841 agaccctgct ggtcgtggac gccatgaccg gccaggaggc ggtcaaggtg gccgaggcct
      901 tccacgcccg gctgccggtg accgggctgg tgctgaccaa gatggacggc gacgcgcgcg
      961 gcggcgccgc gctctccatc cgctcggtga ccgggctgcc gatcagctac atcggcaccg
     1021 gtgagcgcac cgacggcctt gaggcgttcc accccgaccg cctggcacag cgcatcctgg
     1081 gcatgggcga catcctgtcg ctcgtcgagc gggtgcagga gacggtcgac caggacgagg
     1141 ccgagcgggt cgcgcagcgc atgatggagg cgcgcttcac gctcgaggac ttccgcagcc
     1201 agctgaccca gatcaagaag atggggccga tcgggcagct gctggagatg gtgccggggg
     1261 cgggacagct ggccggggcc gcgcagcagg cggtggacga gggacagctg aagcgggtgg
     1321 aggccatcat cgactccatg actcccgagg aacggcggag gccagacctg atcaaggcca
     1381 gccgccggcg gcgcatcgcc atgggcagcg gcacgaccac ggcggacgtc aaccgcctcc
     1441 tcaagcagtt caccgacatg cagaagctca tgcgccagat gagcggcggg cgcatgccca
     1501 acctcggcgg gttgcgcggc atgctgggac gctgacgtgc agcgcctggt gatcgccctc
     1561 accaccctgc tgacgctgac cggcgcggcg gtggtgaccg gctacctgct cgtcttcgcg
     1621 gttgggtccg atcggctggc acaggccacc ccggcaggca ctgcggccta tgccaccgtc
     1681 tacctgcagc cgtcgacggg gcagaagatg aacctggccc agctgctggg tcgcgtcccg
     1741 ggcttcgccg atgccgccag cctcgaccag aagatccacg agatcacggc gcgcctgatg
     1801 ggcgaggccg gcgtcgacta cgagcgggac gtccgtccct ggctggggaa ccaggtcgcc
     1861 atcgcggcgc ggccggatgc cgccgaccca ctggccgccg acacctggtt cctcgcggcc
     1921 accacggacg ccggcgcggc cgaagcggcc ctcgaacgca tcgccaccga ccagggctcg
     1981 caggtcacca cctccgagca cgagggcgtg accgtgagcg ccgccgacgg cacggcgtgg
     2041 gccgtgctcg acgagctggt catcttcgcg ccggatcccg ccagcgtcac ggaggcgctg
     2101 gacgtcgcgg ccggccgtgc cgactcactg gcatccgagg ctggcttccg cgacgccatg
     2161 ggccgcctcc cttcggacca cctggccgcc gtctacctcg actcgaccga agcgcttgcc
     2221 tcctcagagg ccgatacccc cgccactggc tacaccgatc tcagcctggc gctggtcgcc
     2281 gaggcggagg ggctccattt attcggcgtc gctcccttcg acgccgacga agctcccgaa
     2341 tcgatggtgg atgccttcga actcggtcgc caggtgggcg agctcaccgg ctggatgccg
     2401 gaggataccc aggctgccct ggtcgtcttc ggcctgagcc agtccctgac cgctgcggag
     2461 gagcagctcg gcagtgcacc cggcggggat gccgtggccg atgcgctgaa ccaggtccgc
     2521 gcggtggccg cgctggggtt ggggatcgac atcgacgatg atgtcctgcc gctcttcgat
     2581 ggcgagaccg ccatcgcggt cggcgggatc gacaccggta cgccgggggc ccagctcctg
     2641 ctgcggccgt cggacgccca ggcggccgag gcggcgcttg ggcgcctgcg tgacgcgctc
     2701 gccggccggg gagcagccgt cgatgaggag cagatcgacg gcaccacggt gacgacggtg
     2761 gaggtgccgg agctggggac ggtgtcctac gccgtccgcg acggggtggt ggccgccggc
     2821 ctcaccaccg atgcggtgat cggcgccctg gatgccggcg cgggcacctc cctggcggac
     2881 accgagcggt atgccgatgc ctgggagctt gccggcaccc gtggcggcaa cgaaggttac
     2941 ctggacgtcg ccgccgtggt ggatggcttc ggtgacgaga tgggcgccac cggagaggta
     3001 ggcgatatcc tgcgtgagat cggtgcgctg gccgccacca tgccggcgcg ggacgaccac
     3061 agcgagatcc acatcgtcct gaccgtgcgc tgaacgcgga cgggcattta gatcgggtac
     3121 acatcgagga aggaacaaca cttggcagta cgcatccggc tcgcgcgagt gggggcaacc
     3181 aagcgcccca gctatcgcgt cgtggccatc gacagtcgga agccccgtga cgggcgggcc
     3241 ctggagatcc tgggcttcta caacccgctc accgatccgg cgaccgtgca gatcgacgcg
     3301 gacaagctga acgcctggct gtccaagggc gccacgccat cggagacggt ggtcaagctc
     3361 atgcggcagg cggggatcgg tggcgcggcg cctgagacgc cggcagccgc gcccgcgcgc
     3421 cgcaaggccg ccagcggggg caccgcggcc aggtcgcgta gcaagaagga gccggccacc
     3481 gcgccggcgg ccgaagagtg aaggacctgc tgctttacgt cgctcgccac ctggtcgaca
     3541 acgcggacgc cgtcagcgtc gaggtcgagg aggacgagga cgaggtcacc ctggtcctca
     3601 ccgtcgagga gaacgacatg ggccgggtca tcgggcggga tgggcggatc gcgaatgcca
     3661 tccgtaccct gctgcgggtg atgggcgccc gcgacgggcg ccatatcgag ctggagatcg
     3721 tcagcgacca ggagtgagcg gctggcggtc gcgcgcgtgc tgggcgccaa ggggctgtcc
     3781 ggggcgatgc gcgtcgagcc gctgagcgac gttcccggcc ggctggaggt cggatcggtc
     3841 ctcttcatcg agggcgagga tgatccccac cgggtgcgcc tttcggagcc cgccggacgg
     3901 accccggtca tcgccctgga gggcatcgag gaccgttatg gggtcgagcg gctgatcggc
     3961 tcctacctgg aggtcgagcc cgaggcgctc cccgagggta cgtactactg gcaccagctg
     4021 gtgggcctgg aggtgaccga tgaggacggc accccgctcg gcacgctggt cgaggtcttc
     4081 cgtgccggcg agaacgaggt gtaccgcatc gagggcgccg gcggcgagct gctgctgccc
     4141 tcgctgcgcg aggtggtgcg tggcattgac ctggagtccg gaaccatgat cgtccggcgc
     4201 caggacagcg aggaggtctg agctgcgctt cgacgtggtc accctcttcc cggagctgtt
     4261 cgaggtcccg ctgcgcacct cggtggtcgg tcgcgccctg gagcgcggcg tggtggaggt
     4321 cggcgtgcac gacctccggg agcacgggct ggggcggcac cgcagcgtgg acgactaccc
     4381 ctacggcggg ggagcgggca tggtcctccg tccggagccg ctgttcgccg ccgtggagcc
     4441 gctgcgcgcg tctggtgcgc acgtcatcct gctggacccc gccggccagc ggctgaccga
     4501 tgccgtggcg cgcgagctgg cccagctgcc gcacatcacc ctggtctgcg ggcgctacga
     4561 gggcgtcgac gagcgggcgc gcgagctggt ccatcgcgag gtgagcatcg gcgactacgt
     4621 cctcaccggc ggcgagctgc cggccctggt gctgatcgat gccgtggccc ggctggtgcc
     4681 cggcgtcatc gaggaggcct catcggcgtc cgactcgttc tcggagggcc tcctggagca
     4741 cccgcagtac actcgcccgg aggagttccg ggggatggag gtgccgtcgg tcctgctgtc
     4801 gggaaaccac ggggaggtgg accgctggcg ccgacgggag gcgctgcgac gcaccctgga
     4861 gcggcggccg gacctcctgg atgctgcgcc cctgaccggc gacgatcggc ggatgctgga
     4921 ggagctacgc gaggagctgg gggagtaggc gggccgccgt gcggctcacc ctcgccttgc
     4981 ccatcagcgg cggccgctgc tatactccgc cggtcgcggc cgcgtcgtcg gtcgcgttct
     5041 tcacgcccgg agcagtaagc cgctgataag ccggcgataa cgaggtcccc tgtaccatgg
     5101 atgccatcaa ggagctcgag cgcgagcagc tccgcaccga cgtgccgcag atcgccaccg
     5161 gcgacacggt gcgcgtggcg gtgaaggtgg tcgagggcaa ccgcgaacgc atccaggtct
     5221 tcgagggcac cgtgattcgg atgcgcggcg gcggcctgaa ccagcagatc accgtgcgcc
     5281 gcatcgccag cggcgtgggc gtg
//
LOCUS       DATACA010000236         9102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2281020, whole genome shotgun sequence.
ACCESSION   DATACA010000236 DATACA010000000
VERSION     DATACA010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281020"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1344
                     /gene="secY"
                     /locus_tag="VHK28_04875"
     CDS             61..1344
                     /gene="secY"
                     /locus_tag="VHK28_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2141596.1"
                     /translation="MIDSLVTAFRAPDIRRKILFTLGILLIFRVLTNVPVPNVDQQRL
                     ADLFETNQLLALLDLFSGGGLATASIIGMGVNPYINASIIMQLMQGVIPSLGELAREG
                     EYGRNRINQYTRMLTVPLAFAQGFGFSVLLEANGVIPASDLFSWEKVSLLISFTAGTI
                     LLMWLGELISERGLGNGISFIIFAGIVGRVPDQAGGIIGGPDGLIRIIPFILIAIVVI
                     AGVVFINEGQRRIPVQYASRVRGRRMYQGQTQYLPLKVTMAGVIPIIFAISILLFPAQ
                     IAQYFTASENQTVRDVANWIVTNLSQSNVLVYGVLYFVLVVFFTYFYTAFQFRPDQTA
                     DFLRKNGGFIPGIRPGRPTEDFLSRVTNRITLGGALFLATLAVLPLIVSAIIPGTSAL
                     QLGGTSVLIMVSVVVETMKQLEAQMMMRHYEGFMR"
     gene            1341..2018
                     /locus_tag="VHK28_04880"
     CDS             1341..2018
                     /locus_tag="VHK28_04880"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2141597.1"
                     /translation="MTDGDAERRILLLIGAVGAGKGTQAAILSQKLGVPHLSSGELFR
                     RALREGSELGERAREYMERGELVPDEITIGMFMDELAAPAAARGAILDGFPRTRTQAE
                     ALDATLASQGERISLVVYIEVPPEELVARVSGRWVCPTCGTPYHEKTDPPRTPGICDR
                     DGTPLVQRDDDRAEVFRSRLEKQIPPMHEVVQHYDEHGVVTRVDGRRPIDEVTGTILE
                     ALAEAVA"
     gene            2018..2800
                     /gene="map"
                     /locus_tag="VHK28_04885"
     CDS             2018..2800
                     /gene="map"
                     /locus_tag="VHK28_04885"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX2141598.1"
                     /translation="MGMPSRASMVTIKRPEEVARMRHAGLILVDVLDAMRAELRPGVS
                     TADLDRIAEEIITAGGAIPSFKGYGSRPPFPGSICASINDEVVHGIPSPKRKLRDGDL
                     VSLDVGCIWEGWHADCARTWTVGGVPREVEELVDSTRRGMEAGIAAALPGNRLGDVGA
                     AIEAVARERGYGIVRPFVGHGIGQSMHEDPQVPNYGRPGTGLRIEAGMCFAIEPMFNL
                     GGDDVYVDDDGWTVRTDDGALSAHFENTIVVADSGPQILTER"
     gene            complement(2797..3780)
                     /locus_tag="VHK28_04890"
     CDS             complement(2797..3780)
                     /locus_tag="VHK28_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX2141599.1"
                     /translation="MAVQEPAALTARLEDHRTELVGYCYRMLGSSFEAEDAVQETFIR
                     AWRNLESFEGRSALRSWLYRIATNVCLDMLKSRQRRARPMDLRPAGTADDGLPPPLPE
                     SAWIEPVPDSVVVPDGDPAAVAVARESIRLAFIAALQHLPPRQRAVLILREVLRWRAS
                     EVAELLDTTVASVNSALQRARATLEEMQVDLDGGPSRAADGLGEDERQLLSRYVDAFE
                     RYDMDSLTALLAQDATWNMPPFDLWLRTHEDVVKWCLGPGIGCRGSRLVPTQANGAPA
                     FGQYKPAPDGGLEPWSLQVLDVEDGRITGITFFLDTQRFFPMFGLPPRLDA"
     gene            complement(3791..4123)
                     /locus_tag="VHK28_04895"
     CDS             complement(3791..4123)
                     /locus_tag="VHK28_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141600.1"
                     /translation="MDRAGRSLAALLQRDDLPQRFERWWLSLSPGASRSTRATEWTGS
                     LVLVERGVLRVECLSGEQRTFGPGDLLVPGCLPLRTLHNAGDTEVRLVAVRRRASGEP
                     PATAGATR"
     gene            complement(4151..5536)
                     /locus_tag="VHK28_04900"
     CDS             complement(4151..5536)
                     /locus_tag="VHK28_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2141601.1"
                     /translation="MALPITNPKVARDELAAKVAGRIIGPEDPDYDEARTVVMGGIDR
                     RPALIVRPANAQDVAMAIALARESGLELAVRGGGHSAAGHGVSEGGIVIDLRGMKEIE
                     IDAEQRLAWAGGGLTAGEYSIAAAAHGLATGFGDTGSVGIGGITLGGGIGYLVRRHGL
                     TIDSLVAAEIVTADGELLRVDAESHPDLFWAIRGGGGNFGVVTRFQFRLHPLPAFVGG
                     ILVLPATADVIAGFMAAADAAPEELSTIANVMNCPPMPFVPEELHGSLVVMAMMGWSG
                     DLEAGQRAMAPFRALAEPIADMVRPMSYPEMFPPEEGGGYRPLAVAKTMFLRGVDRGR
                     AETIMQYLEASDAPMRAVQLRVLGGAMARVPSEATAFAHRASRVMAVVVSLYIGPDDR
                     AQREAWVDDLLAALDDGEAGAYVNFVGDEGPDRVRAAYPGATYERLAAIKRTYDPTNL
                     FRLNQNVPPAG"
     gene            5764..5982
                     /gene="infA"
                     /locus_tag="VHK28_04905"
     CDS             5764..5982
                     /gene="infA"
                     /locus_tag="VHK28_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX2141602.1"
                     /translation="MAKKDAIEVEGEVVLSLPNAMFKVRLENGHEVLAHISGKLRQNF
                     IRILPGDTVRVELSPYDLTRGRITYRMR"
     gene            5979..6092
                     /gene="rpmJ"
                     /locus_tag="VHK28_04910"
     CDS             5979..6092
                     /gene="rpmJ"
                     /locus_tag="VHK28_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX2141603.1"
                     /translation="MKVSASVKPRCERCKVIKRSGVVMVICVIPKHKQRQG"
     gene            6102..6473
                     /gene="rpsM"
                     /locus_tag="VHK28_04915"
     CDS             6102..6473
                     /gene="rpsM"
                     /locus_tag="VHK28_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2141604.1"
                     /translation="MARIAGVDIPREKRAIISLTYIHGIGPTSAKQIVQAANVSADAR
                     VRDLTEEEVNRLRAVVDSRFKVEGDLRREVSLNVKRLMEIGSYRGLRHRRNLPSRGQR
                     TKTNARQRRGPRRTVGIRRKK"
     gene            6522..6917
                     /gene="rpsK"
                     /locus_tag="VHK28_04920"
     CDS             6522..6917
                     /gene="rpsK"
                     /locus_tag="VHK28_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2141605.1"
                     /translation="MAERKRAARTKKRERKNVPVGHAHIQSTFNNTIVTITDTTGNVI
                     SWGSAGLVGFKGSRKSTPYAAAQTADGAAKRAMEHGMRHIEVFVKGPGAGREQAIRSL
                     QAAGLEVTAITDVTPIPHNGCRPPKRRRV"
     gene            6926..7552
                     /gene="rpsD"
                     /locus_tag="VHK28_04925"
     CDS             6926..7552
                     /gene="rpsD"
                     /locus_tag="VHK28_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2141606.1"
                     /translation="MARYSDAVCRICRREGLKLFLKGTRCYSRKCSFERRSTPPGMNT
                     QRRRKVSEFGLQLREKQKVRKSYSVMERQFRNYFAKAATTKGMTGETLLRMLEMRLDN
                     VVYRMGFARSRAEARQLVSHGHFTVNGRPTNIPSFNAKVGDSIEVRESRRGRELFKTV
                     GETMKAAQIPDWVSVDPARLSGSVLAEPTREQMPMEFNEQLVVEFYSR"
     gene            7575..8597
                     /locus_tag="VHK28_04930"
     CDS             7575..8597
                     /locus_tag="VHK28_04930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX2141607.1"
                     /translation="MIELETTTPQPRIEEIEAQGNVSTFEVNPLADGYGVTLGNALRR
                     VLLNSLEGAAVTSIQVANVFHEFSTLPNVKEDVTQIVLNVKKIRLRSFAQHAVTLKLV
                     KHGAGPVTAGDIAESADVEIVNPDLVLLTLDSDDGSIEMDLTVEAGVGYRAAEHTEDM
                     PIGIIPVDAIFSPVRRVNFVVSDTRVGQMTNFDRLTIEIETDGTTTPKAALSQAADIL
                     VREFGRFTEIGRRPAPGEEEPQAPVAANLLDAPIEELDLPMRAYNSLKRNNITKIGQV
                     LALSDDEFLRMRNFGRKSLDELKERLALRGFISPEQAAPSADESDLPASAELDEAAAA
                     ELDREA"
     gene            8602..8955
                     /gene="rplQ"
                     /locus_tag="VHK28_04935"
     CDS             8602..8955
                     /gene="rplQ"
                     /locus_tag="VHK28_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX2141608.1"
                     /translation="MPHRVTGRRLGRSSSHRLRMLGNLAVAVIRYERVKTTEAKAKEV
                     RGIIDDMIVTAKRGDLSARRALAARMPHEPLIVEKLMSEIATKYADRTSGYTRITKIG
                     ERLGDAAPIVQIELV"
BASE COUNT         1516 a         3168 c         3110 g         1308 t
ORIGIN      
        1 ggggagtcgg atgccgccgt cgaggacgag gcccagggag agcaggggag cgacgcctag
       61 gtgatcgact ccctggtcac cgccttccgc gcgccggaca tccggcgcaa gatcctgttc
      121 acgctgggga tcctgctgat cttccgggtg ctgaccaacg tgcccgtccc gaacgtggac
      181 cagcagcggc tggccgacct gttcgagacg aaccagctgc tggcgctgct ggacctcttc
      241 tcgggtggcg gactggcgac ggcgtcgatc atcggcatgg gggtcaaccc ctacatcaac
      301 gccagcatca tcatgcagct gatgcagggc gtgatcccct ctctgggtga gctggcccgc
      361 gagggtgagt acgggcgcaa ccgcatcaac cagtacacgc gcatgctgac cgtgccgctg
      421 gccttcgccc agggcttcgg cttcagcgtg ctgctggagg ccaacggcgt gatccccgcc
      481 tcggacctct tcagctggga gaaggtctcg ctgctgatca gcttcacggc gggcaccatc
      541 ctgctcatgt ggctgggcga gctgatcagc gaacggggcc tgggcaacgg catcagcttc
      601 atcatcttcg ccgggatcgt gggacgcgtg ccggaccagg cggggggcat catcggcggg
      661 ccggacggcc tgatccggat catccccttc atcctgatcg ccatcgtggt gatcgccggc
      721 gtcgtcttca tcaacgaggg ccagcggcgc atcccggtgc agtacgccag ccgcgtgcgt
      781 ggacggcgca tgtaccaggg ccagacgcaa tacctgcccc tgaaggtgac catggccggc
      841 gtgatcccga tcatcttcgc catcagcatc ctgctcttcc cggcacagat cgcccagtac
      901 ttcaccgcgt cggagaacca gacggtgcgc gacgtggcga actggatcgt caccaacctg
      961 tcgcaaagca acgtgctggt gtacggcgtg ctctacttcg tgctggtcgt gttcttcacc
     1021 tacttctaca ccgcgttcca gttccgcccg gaccagacgg ccgacttcct gcgcaagaac
     1081 ggcggcttca tccccggcat ccgcccggga cggccgaccg aggacttcct gtcgcgggtc
     1141 accaaccgca tcacgctggg cggcgcgctc ttcctggcca ccctggccgt gctgccgctc
     1201 atcgtgtcgg ccatcatccc ggggacctcg gctttgcagc tgggcggcac cagcgtgctg
     1261 atcatggtca gcgtggtggt cgagaccatg aagcagctcg aggcgcagat gatgatgcgg
     1321 cactacgaag gcttcatgcg gtgaccgatg gagatgccga gcggcgcatc ctgctgctga
     1381 tcggcgccgt cggcgcgggg aaggggaccc aggccgccat cctgagccag aagctcggcg
     1441 tgccgcacct ctcctccggg gagctcttcc gacgcgcgct gcgcgagggg agcgagctcg
     1501 gcgagcgcgc ccgcgagtac atggagcgcg gtgagctggt acccgacgag atcaccatcg
     1561 gcatgttcat ggacgagctg gcggcgccgg ccgctgcccg cggggcaatc ctggacggct
     1621 tcccgcgcac gcgcactcag gctgaggcgc tggatgccac gctcgcctcg cagggcgagc
     1681 ggatcagcct ggtggtctac atcgaggtgc cacccgagga gctggtggcg cgggtcagcg
     1741 gccgctgggt gtgccccacc tgcggcaccc cctaccacga aaagaccgat ccgccgcgca
     1801 cgccgggtat ctgcgaccgg gacggcacgc cgctggtgca gcgtgacgac gaccgcgccg
     1861 aggtcttccg ctcgcggctc gaaaagcaga tcccgccgat gcacgaggtc gtgcagcact
     1921 acgacgagca cggcgtcgtg acccgcgtgg acgggcgacg gccgatcgat gaggtgaccg
     1981 gcaccatcct cgaggcgctg gcggaggcgg tggcctgatg ggcatgccct cgcgcgccag
     2041 catggtcacc atcaagcgcc cggaggaggt ggcgcgcatg cgccacgccg ggctgatcct
     2101 ggtggacgtg ctggacgcca tgcgggccga gctgcgtccg ggagtctcca ccgccgacct
     2161 ggaccgcatc gccgaggaga tcatcaccgc cggtggggcg atcccctcgt tcaagggcta
     2221 cggctcgcgt ccgccgttcc ccggatccat ctgtgcctcc atcaacgacg aggtggtgca
     2281 cggcatcccg tcgcccaagc ggaagctgcg tgatggcgac ctggtcagcc tggacgtcgg
     2341 ctgcatctgg gaggggtggc atgccgactg cgcccgaacc tggacggtcg gcggcgtgcc
     2401 gcgcgaggtg gaggagctgg tcgacagcac gcggcgcggg atggaggcgg gcatcgcggc
     2461 cgccctgccc ggcaaccggc tgggcgacgt gggcgccgcc atcgaggcgg tggccaggga
     2521 gcgcggctac ggcatcgtgc ggccgttcgt gggccacggc atcggccagt cgatgcacga
     2581 ggatccgcag gtccccaact acgggcgtcc cgggacgggg cttcgcatcg aggccgggat
     2641 gtgcttcgcc atcgagccga tgttcaacct gggtggcgac gacgtctacg tggacgacga
     2701 cggctggacg gtgcgcaccg atgacggtgc gctgtcggcg cacttcgaga acaccatcgt
     2761 ggtcgccgac tccggcccgc agatcctgac cgagcgttaa gcgtccaggc ggggcggcag
     2821 cccgaacatc gggaagaagc gctgggtgtc caggaagaag gtgatgccgg tgatgcgacc
     2881 gtcctcgacg tccaggacct ggagcgacca cggctccagc ccgccgtccg gcgccggctt
     2941 gtactgcccg aaggccgggg cgccgttggc ctgggtcggc accaggcgcg atccgcggca
     3001 gccgatcccg gggccgaggc accatttgac gacgtcctcg tgggttcgca gccaaaggtc
     3061 gaacggcggc atgttccagg tggcgtcctg ggcgagcagc gccgtcaggc tgtccatgtc
     3121 gtagcgctcg aaggcatcca cgtaccgcga cagcagctgg cgctcgtcct ccccgagccc
     3181 gtcggccgcg cgcgacggcc caccgtccag gtcgacctgc atctcctcca gggtggcgcg
     3241 cgcacgctgc agcgcgctgt tgaccgatgc caccgtggtg tccaggagct ccgccacctc
     3301 ggaggcgcgc cagcgcagca cctcgcgcag gatcagcacg gcgcgctgcc gtggcggcag
     3361 gtgctgcagg gcggcaatga acgccaggcg gatcgattcg cgtgccaccg ccaccgcggc
     3421 cgggtcaccg tccgggacga ccacgctgtc cgggaccggc tcgatccagg ccgactccgg
     3481 aagtggcggg ggcagcccgt cgtccgcggt gccggcgggg cgcaggtcca tggggcgggc
     3541 gcgacgctgt cggctcttga gcatgtccag gcagacgttg gtggcgatgc ggtacagcca
     3601 ggagcgcagc gctgaccggc cctcgaagct ctccaggttg cgccaggcac ggatgaacgt
     3661 ctcctggacg gcatcctcgg cctcgaacga ggagcccagc atgcggtagc agtagcccac
     3721 cagctcggtg cggtgatcct ccagccgcgc cgtgagcgcg gccggttcct gaacggccat
     3781 ccgctgcatg ctagcgcgtc gcgccggcgg tggcaggagg ctcccccgag gcgcgacggc
     3841 gcaccgcgac gagccgcacc tccgtgtccc cggcgttgtg cagggttcgc agcggcaggc
     3901 agccggggac cagcaggtct cccggcccga acgtccgctg ctcaccactc aggcactcga
     3961 cccgcagcac gccacgctcg accaggacca ggctgccagt ccactccgtc gcccgcgtgg
     4021 agcgtgaggc gccgggggac agggagagcc accagcgctc gaagcgctgc ggcaggtcgt
     4081 cgcgctgcag cagcgcggcc aggctgcgcc ccgcgcggtc cacggcagcc accctcccga
     4141 cgagccggcg tcagccggcc ggcggaacgt tctggttgag ccggaacagg ttggtcgggt
     4201 cgtaggtccg cttgatcgcc gccagccgct cgtaagtggc gccgggatag gcggctcgca
     4261 ctcggtccgg gccctcgtcg cccacgaagt tgacgtaggc cccggcctcg ccgtcgtcca
     4321 gggcagccaa caggtcgtcc acccaggcct ctcgctgtgc acgatcgtca ggaccgatgt
     4381 acaacgagac caccaccgcc atcacccggc tggcgcggtg cgcgaaggcg gtcgcctcgg
     4441 acggaacgcg cgccatggcg ccgcccagga cgcgcagctg gacagcccgc atgggtgcgt
     4501 ccgaagcttc caggtattgc atgatggtct ccgcacgccc gcgatccacg ccgcgcagga
     4561 acatcgtctt ggccacggcc agcgggcggt agccgccacc ctcctcgggt gggaacatct
     4621 cggggtagct catgggccgc accatgtcgg cgatcggctc tgccagggcg cggaagggag
     4681 ccatcgcgcg ctggccggcc tcgaggtcgc cgctccagcc catcatcgcc atcaccacca
     4741 ggctgccgtg cagctcctcg gggacgaatg gcatgggcgg gcagttcatg acgttggcga
     4801 tggtcgacag ctcctccggg gcggcatcgg ccgccgccat gaatccggcg atcacgtccg
     4861 cagtggccgg caggaccagg atgccaccca cgaacgctgg cagggggtgc aggcggaatt
     4921 ggaagcgggt gaccaccccg aagttgccgc ccccgccgcg gatcgcccag aacaggtccg
     4981 gatgcgactc ggcgtcgaca cggagcagtt cgccgtctgc ggtgacgatc tcggcggcga
     5041 ccagcgagtc gatcgtcagc ccgtgcctgc gcaccaggta gccgatcccg ccgcccaaag
     5101 tgatgccgcc gatgcccacc gaccccgtgt cgccgaagcc ggtggccaac ccgtgggcgg
     5161 cggccgcgat cgagtactcg ccagcggtca gcccgccgcc ggcccaggcc aggcgctgct
     5221 cggcatcgat ctcgatctcc ttcatgccac gcaggtcgat gacgatgccc ccctcggaga
     5281 caccgtggcc cgccgcggag tgcccgccac cacgcaccgc cagctccagt ccgctctcgc
     5341 gtgccagagc gatcgccatc gccacgtcct gcgcgttcgc gggccgcacg atgagcgcgg
     5401 gacgccggtc gatgccaccc atcacgaccg tccgcgcctc gtcgtagtcc gggtcctccg
     5461 gaccgatgat gcggccggcg accttggccg ccagctcatc gcgcgcgacc ttggggttag
     5521 tgatgggcag agccatggga gagcctcctt gcgttctgga catgcgacga ctccgtgccg
     5581 gacaattcat cggtcatttg gccgattgcc cgttcggcgg tcccgccagg gacgtctggg
     5641 agccaccctt gcacgctcgg agggtcaggg gtatactgcc cgttccgggt cgcccagtgc
     5701 ggcggccttt ccgtgtgtca gtcccattca gaattgtcga agctttacga gggtgcgtgc
     5761 cgattggcga agaaggatgc gatcgaggta gagggcgaag tcgtcctcag cctgccgaat
     5821 gcgatgttca aggtgcggct ggagaacggg cacgaggtgc tcgcgcacat ctccggcaag
     5881 ctgcgccaga acttcattcg catcctgccc ggcgacaccg tccgcgtcga gctcagcccc
     5941 tatgacctca cccggggtcg tatcacctac aggatgagat gaaggtttct gcgtccgtga
     6001 agccccgctg cgagcgctgc aaggtcatca agcgctccgg cgtggtgatg gtcatctgcg
     6061 tcattcccaa gcacaagcag cggcaggggt agcaacagct cgtggcacgt attgctggcg
     6121 tcgacatccc gcgcgagaag cgcgcgatca tcagcctcac ctacatccac ggcatcggtc
     6181 ccacgtcggc caagcagatc gtgcaggccg ccaacgtgtc cgccgacgcc cgggtgcggg
     6241 acctgaccga ggaggaagtc aaccgactga gagcagttgt cgactcgcgg ttcaaggtgg
     6301 agggcgacct gcgccgcgag gtgagcctga acgtcaagcg cctgatggag atcggctcct
     6361 atcggggcct ccgtcaccgc cgcaacctgc cctccagggg gcagcgcacc aagaccaacg
     6421 cccggcagcg acgcggcccg cgccgcacgg tcgggatccg gcgcaagaag tagtgaacgc
     6481 aacaggccga tacgcatcgg cgaactcgaa tcaggagtcc catggctgaa agaaagcgcg
     6541 cggcacgcac caagaagcgc gaacgcaaga acgtgccggt cgggcacgcc cacatccagt
     6601 cgacgttcaa caacaccatc gtgaccatca ccgacacgac cgggaacgtc atcagctggg
     6661 gcagtgccgg cctggttggc ttcaagggct cgcgcaagag cacgccgtac gccgccgccc
     6721 agaccgccga cggcgccgcc aagcgcgcca tggagcacgg catgcggcac atcgaggtct
     6781 tcgtcaaggg acccggtgcc ggtcgcgagc aggccatccg ctccctgcag gccgccggcc
     6841 tggaggtcac cgccatcacc gacgtgacac ccattccgca caacggctgc cgcccgccca
     6901 agcggcgccg ggtctagggg gacgcatggc acgctattcg gacgccgtct gccgcatctg
     6961 ccgccgcgag gggctgaagc tcttcctgaa gggcacgcgc tgctacagcc gcaagtgctc
     7021 cttcgagcgg cgcagcacgc cgccgggcat gaacacccag cgtcgccgca aggtgagcga
     7081 gttcggcctg cagcttcggg agaagcagaa ggtccgcaag tcgtactcgg tcatggagcg
     7141 ccagttccgc aactacttcg ccaaggcggc caccaccaag ggcatgaccg gcgagacgct
     7201 gcttcgcatg ctggagatgc gcctggacaa cgtggtgtac cgcatgggct tcgcccgctc
     7261 gcgcgccgag gcgcgccagc tggtcagcca cggccacttc accgttaacg ggcggcccac
     7321 caacatcccg tccttcaacg ccaaggtggg cgacagcatc gaggtgcgcg agagccgccg
     7381 cggccgtgag ctgttcaaga cggtgggcga gaccatgaag gcggcgcaga tcccggactg
     7441 ggtgagcgtc gatccggccc ggctgtcggg cagcgtcctc gccgagccga cccgcgagca
     7501 gatgcccatg gagttcaacg agcagctggt cgtcgagttc tactcgcgat agcagcagga
     7561 gtcaccaccc gatcatgatc gagctagaaa cgacaacgcc gcagccccgc atcgaggaga
     7621 tcgaggcgca ggggaacgtc tcgaccttcg aggtgaaccc actcgcggac gggtacggcg
     7681 tcaccctggg caacgcgctg cgccgggtcc tcctcaactc gctggagggc gcggcggtga
     7741 cctccatcca ggtcgcgaac gtcttccacg agttcagcac cctgccgaac gtcaaggagg
     7801 acgtcaccca gatcgtcctg aacgtcaaga agatccgcct ccgatccttc gcccagcacg
     7861 ccgtcaccct caagctggtg aagcacggcg ccgggccggt caccgccggt gacatcgccg
     7921 agtcggcgga cgtggagatc gtgaacccgg acctggtcct gctcacgctc gacagcgacg
     7981 acggctcgat cgagatggac ctcaccgtgg aggcaggcgt cggctaccgc gccgccgagc
     8041 acaccgagga catgccgatc ggcatcatcc cggtggacgc catcttctcg ccggtgcgcc
     8101 gcgtgaactt cgtggtcagc gacacccgcg tgggccagat gacgaacttc gaccggctca
     8161 ccatcgagat cgagaccgat ggcaccacca cgccgaaggc cgcgctttcc caggcggccg
     8221 acatcctggt ccgcgagttc ggccgcttca ccgagatcgg ccggcgaccg gctcccggcg
     8281 aggaggagcc gcaggctccc gtcgccgcca acctgctgga tgcgcccatc gaggagctgg
     8341 atctccccat gcgcgcctac aacagcctga agcgcaacaa catcaccaag atcggacagg
     8401 tgcttgcgct gtcggacgac gagttcctgc gcatgcggaa cttcgggcgc aagagcctgg
     8461 acgagctgaa ggagcggctc gccctgcgcg gcttcatcag tcccgagcag gccgcgccga
     8521 gcgcggacga gtcggacctt ccggcctcgg ccgagctgga cgaggcggct gccgccgagc
     8581 tggaccggga ggcctagacc catgccgcat cgggtcaccg gtcgccggct gggccggagc
     8641 agcagccacc ggctgcgcat gctcggcaac ctggccgtgg ccgtcatccg ctacgagcgg
     8701 gtgaagacca ccgaagcgaa ggccaaggag gtgcgcggca tcatcgacga catgatcgtc
     8761 accgccaagc gtggggacct ctcggctcgc cgggccctgg cggcgcggat gccccacgag
     8821 ccgctcatcg tcgaaaagct gatgagcgag atcgcgacca agtacgcgga tcgcacctcc
     8881 ggctacacgc gcatcaccaa gatcggcgag cgtctgggcg acgccgcgcc gatcgtccag
     8941 atcgagctcg tctgagcgca gcagagcgca cgggcgagcg aacaaccgcg gacggactcg
     9001 tggctggccg agcgcaacgc tgcgcgcggg ccgtgatcga gtacgacggg accgacttcg
     9061 ccggctccca gtaccagccg aaggctcgga cggtgcaggg gg
//
LOCUS       DATACA010000237         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1558177, whole genome shotgun sequence.
ACCESSION   DATACA010000237 DATACA010000000
VERSION     DATACA010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558177"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VHK28_04940"
     CDS             <1..407
                     /locus_tag="VHK28_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2141609.1"
                     /translation="HLRAEGNGKPITAVLTSGQRHEQIALEAVLDRGAIRRQGRGRPR
                     LRPRRVAGDKGYSSPTARGRIRRRGIRPVIPTKSNEPRDPAFDREAYRERNRIERLIN
                     RLKQFRRIATRYEKRAINYLAMLTIGMSLLWL"
     gene            465..1289
                     /locus_tag="VHK28_04945"
     CDS             465..1289
                     /locus_tag="VHK28_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2141610.1"
                     /translation="MWTAQDRERYKDDGRRYPSDLSDAEWALVRPLIETYPTLTKDRR
                     EMVNGCLYLAAEGCRWRSLPKEFGPWQTVRGYWDRFHRDGLWADLATLLTPAARARLG
                     RKPEPSTGIVDSQSVASGPQKGARGIDANKKVKGIKRHALTCSVGFLLAVFVSAASLH
                     DTHGLAPLLEQANATGWSLERIKVDGIYTGPTVRAAAARHDVEVQVVRRDPASRGFAP
                     LPLRWRIEATFGTLTNRYRRLTRHLEQSADAAENLVEVANLRRVLRVLTRPPQEQV"
     gene            1358..1858
                     /locus_tag="VHK28_04950"
     CDS             1358..1858
                     /locus_tag="VHK28_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141611.1"
                     /translation="MDERSKKTVHGVRTFTPEDAMALRSTLCLTLSPEQRAALERLRR
                     VHHTPQQLALRARMILLAGDGRGVRETARLVGVEAKTVRLWRRRWLAATDADGVADRL
                     ADAPRSGAPATFTAEQVCAIVALACEPPSARDARSPTGARARWPARRSSAGWSARSRS
                     ARSGAC"
     gene            1886..2617
                     /locus_tag="VHK28_04955"
     CDS             1886..2617
                     /locus_tag="VHK28_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2141612.1"
                     /translation="MRLWLTPKPDPEFQAKGAEICTVYQQAATASAEAPTRTVSIDEM
                     SGVQALEKIAPSLPIKPGQVERQEFEYRRHGTQTLIASFDVATGQVHGTVGDRRTEED
                     FARFLDGLLASAPDTVRWHIVCDNLDIHLSESVVRLVARHGGLAEDLGVKGKVGILQS
                     RASREAFLRDPGHRIVFQFTPKHASWLNQSEIWFSILARKVIRRGSVTSTEDLRAKIE
                     GFIAYFNRTMAKPFRWTYRGKPLAA"
BASE COUNT          464 a          960 c          931 g          390 t
ORIGIN      
        1 tccacctgcg cgccgagggc aacggcaagc cgatcacggc ggtgctgacc tctggccagc
       61 ggcatgagca gatcgcgctg gaagcggtgc tcgatcgagg cgcgatccgc cgccaggggc
      121 gtggtcgtcc gcgcctgcgc ccccggcggg tggccggcga caagggctac agcagcccca
      181 ctgcccgagg gcggatccgg cgccgcggca tcaggccggt gatcccgacc aagagcaacg
      241 agccgcgcga tccggccttc gaccgcgagg cgtaccgaga acgcaaccgc atcgagcgac
      301 tgatcaaccg gctcaaacag ttccgacgga tcgccacccg ctacgaaaag cgcgccatca
      361 actacctcgc catgctcacc atcggcatga gcctgctctg gctctgagag cctgttttac
      421 cgggacgacc ctatttccgt cgtgacgaac gacggagggg caagatgtgg accgcacaag
      481 atcgcgagcg ttacaaggac gatggccgac gctacccgag cgatctgagc gacgcggaat
      541 gggcgctggt gcggccgctg atcgagacct acccgacgct gacaaaagac cgccgcgaga
      601 tggtcaacgg ctgcctttac ttggcggccg agggctgccg ctggcgctcg ctgccgaaag
      661 agttcggccc ctggcagacg gtccgcggct attgggatcg cttccaccgc gatggcctct
      721 gggctgatct cgccaccctg ctgaccccgg cggcccgggc gcgcctcggc aggaagcctg
      781 agccctcgac cgggatcgtg gattcgcaaa gcgtcgcgtc cgggccgcag aaaggggcgc
      841 gcggcatcga tgcgaacaag aaggtgaaag gcatcaagcg ccatgccctg acctgctcgg
      901 ttggcttcct cctggccgtg ttcgtctcgg ccgccagtct gcacgacacg cacgggctgg
      961 cgccgctctt ggaacaggcc aatgccaccg gctggagcct cgagcggatc aaggtcgacg
     1021 gcatctacac ggggccgacg gtgcgcgcgg cagcggcgcg ccacgacgtc gaggtccagg
     1081 tcgtgcgccg cgatccggcc agccgcggct tcgccccgct gccgctacgg tggcggatcg
     1141 aggcgacctt cggcacgctg accaatcgct accggcgctt gaccagacat ctggagcaga
     1201 gtgctgatgc ggcagagaat ctcgtcgagg tcgccaatct caggcgtgtc ctccgcgtcc
     1261 tcacacggcc acctcaagaa caggtatgat gaaacaggct ctgagctttg cagacacgcc
     1321 ctagtacagg tcagcggaag agcctgcccc atcgcgcgtg gacgagagga gcaagaaaac
     1381 cgtccatggc gtgaggacgt tcacgccgga ggacgcgatg gcgctgcgct ccaccttgtg
     1441 cctcaccctg agccccgagc agagagcggc gctggaacgc ttgcgccggg tccaccacac
     1501 gccccagcag cttgccttgc gggctcggat gatcctgctg gccggggacg gcagaggcgt
     1561 gcgcgagacc gcccgcctcg tcggggtcga ggccaagacg gtccgccttt ggcggcggcg
     1621 ctggctcgcg gccacggacg cggacggcgt cgccgaccgt ctcgccgacg cgccacgctc
     1681 gggtgcgccc gcgaccttca ccgccgagca ggtgtgcgcg atcgtggccc tggcctgcga
     1741 gccaccctcc gccagggatg cccgatcacc cactggagcc agagcgcgct ggcccgcgcg
     1801 gcggtcgagc gcgggctggt cggccagatc tcgcagcgct cgatcgggcg catgctaaaa
     1861 agaggccgac ctccagccac atcgtgtgcg gctctggctc acgcccaagc ccgaccctga
     1921 gttccaggcc aagggcgctg agatctgcac ggtctatcag caggccgcca ccgcctcggc
     1981 cgaggcgccg acgcgcacgg tgtcgatcga cgagatgtcc ggcgtgcagg cgctggagaa
     2041 gatcgcgccg tccttgccga tcaagccggg gcaagtggaa cggcaggagt tcgagtaccg
     2101 ccgccatggc acccagacct tgatcgccag cttcgacgtc gcgaccgggc aggtccacgg
     2161 cacggtcggc gaccgccgca ccgaggagga cttcgcccgc tttctcgacg gcctgctcgc
     2221 gagcgcgccc gacaccgtcc gctggcacat cgtttgcgac aacctggaca tccacctctc
     2281 cgagagcgtg gtccggctgg tggctcggca cggcggcctg gcggaggatc tcggggtgaa
     2341 agggaaggtc ggcatcctgc agtcgcgggc cagccgggaa gcgttcctgc gcgatcccgg
     2401 gcaccgcatc gtcttccagt tcacccccaa gcatgcctcc tggctcaacc agagcgagat
     2461 ctggttctcg atcctggccc gcaaggtgat ccgtcgcggc agcgtcacct cgaccgagga
     2521 cctcagggcc aagatcgagg gcttcatcgc ctacttcaac cggaccatgg ccaagccctt
     2581 ccgctggacc tatcgcggca agccgctcgc cgcgtgagcc atggaggccg gacttccgct
     2641 cgggtgcact agcagggcca cgcggagggc ggcgcttggc tcgagggctc gctgtgggag
     2701 aagccggcgg ccgcgccatc gacgcggtcg tgacccggcc gggca
//
LOCUS       DATACA010000238         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_835144, whole genome shotgun sequence.
ACCESSION   DATACA010000238 DATACA010000000
VERSION     DATACA010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835144"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..356)
                     /locus_tag="VHK28_04960"
     CDS             complement(147..356)
                     /locus_tag="VHK28_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141613.1"
                     /translation="MPELKPNPYQHRRPRRPAYLPRRTTPPPERREPPDMRYLLYVGI
                     ALAAVMAALAMPYLVSYLLQALGLV"
     gene            complement(356..2749)
                     /locus_tag="VHK28_04965"
     CDS             complement(356..2749)
                     /locus_tag="VHK28_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX2141614.1"
                     /translation="MTAVQPPTLSADELYRRATGSFDDWELVKDVIDEVIDLALNYRQ
                     SGHPGGSRSKVHLFLSLLLSGAMRWDIRRPWLPFGDRFVLSAGHTVPLVYATLAVLNE
                     TLRARQERDGDDRFAFPDDGKWALTWEDLLKLRRRGGLAGHAEMEGKTLFLKFNTGPS
                     GHGMPPAAGEAVALKLAGAGEVKVFAVEGEGGLTPGASHETRNSAWGLGLSNLVFLVD
                     WNDYGIDDVPISAVVHGTPPEWFEPYGWRITGTESGSEWGPVTRAVLEAARGENPAGV
                     PSMAWFKTRKGRGYGKYDNKSHGSPHAMNSPEFWEVRRGFMERHGVEYAGVGEPAPTD
                     AAEREAQARHNFGVAVGVLRDNAAVVDAISDRLLELGASVPERIDGFHIGGKGTAIFD
                     DSRFTDVAAYPPEIWKRPGEKAPNRAALAAWGAWVNATARAEYDRPLVIACSADLAES
                     TNIAGFAKDWEDLPGWGWYDREKNPRGALLPQEITEFTNAGITVGIATVNLADDPFGA
                     FNGFWAACSTYGSFSYLKYGPMRLFSQLAQDCNLKVGKVIWVAGHSGPETAEDSRTHF
                     GIFATGVTQLFPEGHVIDLHPWEYNEVPVVLAAALSTEVPIVALHLTRPSVEIPDREA
                     MGMDSHLAAARGAYVLRRPSPDRPCAGTVYVQGTMSTANLVKVLPDLDREGLNVRVVA
                     AISPQLFRRQDAAYRDEICSPADRWDGMAVTNRAFKLMRDWVDGPIAEEYSLSSDWDN
                     RWRTGGTVDEVIDEAHLGTGHILEGIERYVRDREARLRRLRDLVDSVERGHAAGNGA"
     gene            complement(2746..>3864)
                     /locus_tag="VHK28_04970"
     CDS             complement(2746..>3864)
                     /locus_tag="VHK28_04970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX2141615.1"
                     /translation="RDVIHLEIGEPDFDTPRHISEAAARGLLDERLTHYTAATGIAPL
                     REAIAADVKRWKGIDATPDQVVVTPGAKPIMFYAMLALVDEGDEVIYPNPGFPIYESM
                     ARYVGGTPVAAPLREENDFRMDVAEVASLVTDRTRLLVINSPHNPTGSILTADGIREL
                     ARIALEHDLVVLTDEIYGRLQYGGEPLSIATLPGMAERTITLDGFSKTFAMTGWRLGY
                     GIVPEWLLPSFSRLVINSVSCTNAFAQAGAVAALTGPQDEPERFKQEFLARRQLIVDG
                     FNAIPGVRCVMPQGAFYAFPNMSSFGRSSAEIADHLLYDAGVCGLAGTAFGEHGEGYL
                     RFSYANSRENIGRALERIGDSLSRLERTGELAGAGTGS"
BASE COUNT          566 a         1354 c         1367 g          577 t
ORIGIN      
        1 cgcagcaagt ggcgggagac aggcggctat gcggacgacg agcgggcgta cccgatcgag
       61 ctgccacgcc tcatccggcc gcgcgtccga gtcgacggcg accgcgtgct ggtctggacc
      121 gccagaacct gaccgaatgc aaaacctcag accaatccga gcgcctggag caggtagctg
      181 accaggtaag gcatcgccag ggcggccatc accgccgcca gcgcgatccc cacgtacagc
      241 aggtaacgca tgtcgggtgg ctcgcgccgc tccggcggcg gggtcgtgcg gcgcgggagg
      301 taggccggtc gccgcgggcg gcggtgctgg tacgggttcg gcttgagctc gggcatcagg
      361 ctccgttgcc ggcggcgtgc ccgcgctcca ccgagtcgac caggtcacgc agccgtcgca
      421 gccgcgcttc gcggtcgcgg acgtagcgct cgattccctc caggatgtgc ccggtcccca
      481 ggtgcgcctc gtcgatcacc tcgtccaccg tgccgccggt ccgccatcgg ttgtcccagt
      541 cagagctgag cgagtactcc tcggcgatcg gcccgtccac ccagtcacgc atcagcttga
      601 aggcgcggtt ggtgaccgcc atgccgtccc atcggtccgc cggggagcag atctcgtcgc
      661 gataggccgc atcctggcgg cggaacagct gcgggctgat ggcggcaacc acccggacgt
      721 tgaggccctc gcggtccagg tcgggcagca ccttcaccag gttggcggtc gacatggtgc
      781 cctgcacgta caccgtgccg gcgcagggcc ggtccgggga cgggcggcgc aggacgtagg
      841 ccccgcgcgc ggcggccaga tgggagtcca tgcccatcgc ctcgcggtcc gggatctcga
      901 ccgacggacg ggtcaggtgc agcgccacga tcggcacctc ggtggagagc gcggcggcca
      961 gcaccaccgg cacctcgttg tactcccacg gatgcaggtc gatgacgtgg ccctccggga
     1021 agagctgggt cacgccggtg gcgaagatgc cgaagtgggt gcggctgtcc tccgcggtct
     1081 ccgggcccga gtggccggcc acccagatga ccttgcccac cttgaggttg cagtcctggg
     1141 ccagctggct gaagaggcgc atcggtccgt acttgaggta cgagaaggac ccgtacgtcg
     1201 agcacgccgc ccagaacccg ttgaaggcac cgaaggggtc gtccgcgagg ttgacggtgg
     1261 cgatgccaac ggtgatgccg gcgttggtga actcggtgat ctcctgcggc agcagcgccc
     1321 cgcgcgggtt cttctcgcgg tcgtaccagc cccagccggg gaggtcctcc cagtccttgg
     1381 cgaagccggc gatgttggtc gattcggcca ggtcggcaga gcaggcgatc accagcggac
     1441 ggtcgtactc ggcacgcgcc gtggcgttga cccaggcgcc ccaggcggcc agcgccgcgc
     1501 ggtttggcgc cttctcaccg ggccgcttcc agatctcggg tggataggcc gcgacgtcgg
     1561 tgaagcgcga gtcgtcgaag atggccgtgc cctttccacc gatgtggaag ccgtcgatcc
     1621 gctccgggac ggatgccccc agctccagca ggcggtcgct gatggcgtcc accacggcgg
     1681 cgttgtcgcg cagcacgccg accgccaccc cgaagttgtg gcgagcctgc gcctcccgct
     1741 ccgcggcgtc ggtgggggcc ggctcgccga cgccggcgta ctccaccccg tggcgctcca
     1801 tgaacccgcg gcgcacctcc cagaactccg gagagttcat ggcgtgcggc gacccgtggc
     1861 tcttgttgtc gtacttgccg tagccgcggc ccttccgcgt cttgaaccag gccatggagg
     1921 gcactccggc agggttctcg ccgcgcgccg cctccagcac ggcgcgggtg accggccccc
     1981 actccgaccc cgactcggtc cccgtgatgc gccagccgta gggctcgaac cattccggtg
     2041 gcgtgccgtg caccacagcc gagatgggca catcgtcgat gccgtagtcg ttccaatcca
     2101 ccaggaagac gaggttcgac agaccgaggc cccacgcgct gttgcgagtc tcgtggctgg
     2161 cgcccggcgt caggccaccc tcaccctcca cggcgaagac cttgacctca ccggcaccgg
     2221 ccagcttcag cgccaccgcc tcgccggcgg cgggcggcat gccgtggccg gaggggccgg
     2281 tgttgaactt gaggaagagg gtcttgccct ccatctccgc gtgcccggcc agcccgccgc
     2341 ggcgtcgcag cttgagcagg tcctcccagg tcagcgccca cttgccgtcg tccgggaagg
     2401 cgaaccgatc gtccccgtcg cgctcctgcc gcgctcgcag cgtctcgttg agcacggcca
     2461 gcgtggcgta caccagcggc acggtgtggc ccgcggacag cacgaagcga tcgccgaacg
     2521 gcagccacgg gcgcctgatg tcccagcgca tggcgcccga cagcagcagc gagaggaaca
     2581 ggtgcacctt cgagcgcgaa ccgccgggat gcccggactg ccggtagttg agggcgaggt
     2641 cgatgacctc gtcgatcacg tccttgacca gctcccagtc gtcgaacgag ccggtcgccc
     2701 ggcggtagag ctcgtcggcg gagagcgtcg gcggctggac ggcggtcacg agccggttcc
     2761 cgcgccggcc agctccccgg tccgctccag ccgcgacagc gagtcgccga tgcgctccag
     2821 ggcacggccg atgttctccc gcgagttcgc gtagctgaag cgcaggtagc cctctccgtg
     2881 ctccccgaag gcggtgccgg ccaggccgca gacgcccgcg tcgtagagca ggtggtcggc
     2941 gatctccgcg ctgctgcgcc cgaagctgga catgttcggg aaggcgtaga acgcgccctg
     3001 gggcatgacg cagcgcacgc ccgggatggc gttgaagccg tccacgatca gctgccggcg
     3061 ggccaggaac tcctgcttga agcgctccgg ctcgtcctgc gggccagtca gggcggcgac
     3121 cgcgccggcc tgggcgaagg cgttggtgca cgacacgctg ttgatcacca gtcggctgaa
     3181 cgagggcagc agccactcgg gcacgatccc gtatcccagc cgccagccgg tcatggcgaa
     3241 cgtcttcgag aagccgtcca gggtgatcgt ccgctcggcc atgcccggca gggtcgcgat
     3301 cgagagtggc tcgccgccgt actgcagccg gccgtagatc tcatcggtca ggaccaccag
     3361 gtcgtgctcc agcgcgatgc gcgccagctc gcgaatgcca tccgcggtca ggatggaccc
     3421 cgtcggattg tgcggcgagt tgatgaccag cagacgtgtg cggtcggtca ccagcgaggc
     3481 gacttcggcc acgtccatgc ggaagtcgtt ctcctcgcgg aggggagccg cgaccggcgt
     3541 gccgcccacg tatcgggcca tggactcgta gatggggaac cccgggttgg ggtagatgac
     3601 ctcgtcgccc tcgtcgacca gggccagcat ggcgtagaac atgatcggct tggcgccggg
     3661 agtgaccacc acctggtccg gcgtggcgtc gatccccttc cagcgcttga catcggccgc
     3721 gatcgcctcg cgcagtggcg cgatgccggt ggccgcggtg tagtgggtca ggcgctcgtc
     3781 cagcaggccg cgcgcggcgg cctcgctgat gtggcgcggg gtgtcgaaat cgggctcgcc
     3841 gatctccagg tggatcacgt cgcg
//
LOCUS       DATACA010000239         8762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2119775, whole genome shotgun sequence.
ACCESSION   DATACA010000239 DATACA010000000
VERSION     DATACA010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119775"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..680)
                     /locus_tag="VHK28_04975"
     CDS             complement(231..680)
                     /locus_tag="VHK28_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HEX2141616.1"
                     /translation="MAEELRFFLRTAVYSAGIAVIYWFSSHDPVTDAYDWAGTALLAA
                     AALATGATVAVAAAFARGALHGGGARSPVGAVIDWIGLTDPGGRADAQPLATGLDQLP
                     RTSSWPLVAGVAATLIGLGLVYGAWLWMPGAALLALSVWGWVTQLRG"
     gene            complement(673..2301)
                     /gene="ctaD"
                     /locus_tag="VHK28_04980"
     CDS             complement(673..2301)
                     /gene="ctaD"
                     /locus_tag="VHK28_04980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX2141617.1"
                     /translation="MSTVAHVRPQRSAFVDYLTTVDHKRIGIMYLVTAIGFLGLGGVL
                     GLIMRAELSFPGLQVVDEATYNQLFTMHGTGMIFFAIVPLGIGFANYLLPLQIGAPDV
                     AFPRLNALTYWLFLLGGLVAFSGFLTVSGAASFGWYGYPPLSTDTYSPGAGGDLWVMG
                     LILSGTSGILGAVNFIATVFTMRAPGMTMFRIPIFTWNILVTALLIILTFPVLTAALV
                     MLFIERTTGAVFFDPAAGGSSVLWQHLFWFFGHPEVYIMALPFFGVVSEVVPVFSSKR
                     IFGYRALVFATLLIGAYSFSVWAHHMFTTGAVSNVFFAVTSFLIAIPTGVKFFTWIAT
                     MWRGRIRFSTPMLFVFGFLFQFLAGGITGVFLASPPIDYQVHDSYYVVAHMHYVLFGG
                     SAFAAFAAFYFWIPKMSGRMLSEGLGKVHFWLLVIGFNLTFFPMHQLGLDGMARRIAD
                     YPAETGWSGVNVLISIGAMIIALATLLFIWNVVRSVWMRRGAPAGNDPWGGYSLEWYT
                     SSPPPHHNFTALPAIRSERPTYDARRAAEEVADG"
     gene            complement(2298..3017)
                     /gene="coxB"
                     /locus_tag="VHK28_04985"
     CDS             complement(2298..3017)
                     /gene="coxB"
                     /locus_tag="VHK28_04985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX2141618.1"
                     /translation="MGRTFAAVCRAIALPVMAAILAGCVPEPVTTEGARVGTLYNIFL
                     VAAAIVFVVVAGLIGWSIVRYRATDDERRPSDTHENIRLELLWWALPTLLVIGLIILT
                     AQVLSEVDRRTEDPDLTVTVTGFQWQWRFAYEGTDTVITGITGEPPVLVLPVGRRIAF
                     DLESPDVIHSFWVPEFLIKRDVLPDKTNRIELTIEEEGTYRGVCGEFCGLLHDRMLFS
                     IDAIPADRFDTWLASGGEAGS"
     gene            3118..3702
                     /locus_tag="VHK28_04990"
     CDS             3118..3702
                     /locus_tag="VHK28_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2141619.1"
                     /translation="MVTYDESTALLVVDVQNDFADPGGSLYVRGGEELIPRINDEVQR
                     AVAAGALVVYTQDWHPESTPHFAKDGGVWPVHCVMDTWGAELHPQLVVAGERIRKGTN
                     GEDGYSGFTMRDPVSGEQVPTPLDGMLREQGIRSVVVAGLATDYCVCSTVIDAQRLGY
                     RGVVLTEASAAVDLQPGDGERRLQEAAAAGAELV"
     gene            3702..5051
                     /locus_tag="VHK28_04995"
     CDS             3702..5051
                     /locus_tag="VHK28_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX2141620.1"
                     /translation="MRRAAYALIALVGLTLLVAAPARAGDGDVHVITIDRDINPVSAR
                     YLIRQLDEAVEEGAAAVLVEINTPGGLLDATQDITTAMLGAQVPVIAYVTPPGTRAAS
                     AGVFVTMSAHVAAMAPSTRMGAATPVSSEGGDIPEDLRNKIIADTVEYARSLAEARGR
                     NADWAESAVVDADVVGAEEAVELDVVDLIAPDRRALLEEIDGREVTLGDGSTVTLRTA
                     DAPAVEREMSPFEQLLMVLANPNVAVLLLSIGSIGIYFELANPGTFFPGITGAIALIL
                     GLFSLGSLPINYAGLALLLLGLTLLGAEIWVASGGILGIGGGIAFLLGSFILIDDTRA
                     PLLEVSRPLILGITLVLVGFVVFALQAVMRARHRPAYIGGGDLVGREASVRGPDAVYI
                     AGELWSARPASGEGSLTPGRRVRVVDREGLHLLVEELPETDPDVREADAGQPTEEGT"
     gene            5052..5900
                     /locus_tag="VHK28_05000"
     CDS             5052..5900
                     /locus_tag="VHK28_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2141621.1"
                     /translation="MEELLQILPVIVVALVAIAWIVGAAVKVVQEYERGVIFRLGRLI
                     GAKGPGLFFIIPIIDRMVKIDLRTVTLDIPSQEAITRDNVTVRVNAVCYFRVVDPEAA
                     VVRVENFLLATTQIAQTTMRSVLGQSELDELLSKREEINARLQQIIDEQTEPWGVKVS
                     VVEVKDVELPQTMQRAMARQAEAERDKRAKVIHAEGEFMAAQQLSEAANIINSAPSAL
                     QLRYLQTLSDISSDQSRTIIFPLPMDLISAFIGTRADGESGGGGGGSNSPVKPVTPTT
                     GGQATE"
     gene            6150..6479
                     /locus_tag="VHK28_05005"
     CDS             6150..6479
                     /locus_tag="VHK28_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX2141622.1"
                     /translation="MAYTDQVLTCADCGIDFVFSASEQEFFAQKGFTSAPKRCPSCRA
                     QRRASGGGSSYGNGGGYGGDTGYSRGPREMFDAVCARCGKDTQVPFRPTGARPVYCSD
                     CFRLVRG"
     gene            6920..>8762
                     /locus_tag="VHK28_05010"
     CDS             6920..>8762
                     /locus_tag="VHK28_05010"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2141623.1"
                     /translation="MTDLEQWRELGQQLRVDSIRSSSAAGSGHPTSSMSAADLMAVLM
                     ARHLRYDFAAPDNPNNDHLIFSKGHASPLLYAMYKAAGALSDEEMLTFRELGSRVEGH
                     PTPVLPWVDVATGSLGQGLPIAVGVAYAGRNLDRLPYRVWCLTGDSEMAEGSMWEAFE
                     AAGFHGLGNLTAIIDVNRLGQTGETMHGWDLDAYARRARAFGWHTVEIDGHDVEAIDR
                     AYAEAAGIEDRPTAIIARTEKGHGVEAVANQNGAHGKPVSDEAAAIEELGGPRQLNIS
                     VTPPEGTGEPHRFPTQGELKLPSYEPGTKEATRKAYGDALLALGQARGDVVAIDGEVG
                     NSTYSELFAKELPERFVQAYIAEQMMLSYAVGFQVRGWRPFAATFAAFLSRAYDFVRM
                     AAVSRANIVLAGSHAGVSIGEDGPSQMALEDLASLRAVHGSTVLYPSDANQAADLLAQ
                     MADRTGIVFMRTTREKTPVIYQPGERFPIGGSRLVRRTDDDQVTLVGAGITLHEAIKA
                     ADALASDGISARVIDLYSVKPLDAETLRAAASQTAGILTVEDHYPEGGIGDAVLDALA
                     GDGLGVPVRKLAVRIMPGSAKPEEQLAAAGIDAKHIADAARVRTSSRA"
BASE COUNT         1461 a         2909 c         3125 g         1267 t
ORIGIN      
        1 gcgccttcca ggcggtgagg tgggcctgat ggtccttcgg cgaccagtcg ccgggacgga
       61 tcgggtcgtc gcgaacaccg tggtcgagcg cgccgaagat atcggcctcc gcacggcgca
      121 tcgagcgcag catctccacc agcgtggcgt tgagggtctg gggatggtcc ataggcggga
      181 accataccgc tatagcgggc cggacgattc gcccggagcg ccgcgtttgg ctagccgcgc
      241 agttgcgtga cccagcccca cacgctgagg gccagcaggg cagcgccggg catccacagc
      301 caggcgccgt acaccaggcc cagcccgatg agggtggcgg ccaccccggc caccaggggc
      361 caggacgagg tgcgcggcag ctggtcgagg ccggtggcca ggggctgtgc atcggctcgc
      421 ccgccgggat cggtcagccc gatccagtcg atcacggcac ccaccgggga ccgcgcgcca
      481 ccaccgtgaa gcgcgccgcg cgcgaaggcg gcggccaccg ccaccgtggc gccggtcgcc
      541 agcgcggcgg cggccagcag cgcggtgccg gcccagtcgt aggcatcggt caccgggtcg
      601 tggctggaga accagtagat gacggcgatg ccggccgagt agacggcggt gcgcaggaag
      661 aagcggagct cctcagccat cggccacctc ctcggcggcg cggcgggcgt cgtaggtggg
      721 gcgctcggag cggatggctg gcagggccgt gaagttgtgg tgcggcggcg gcgaggaggt
      781 gtaccactcc agcgagtagc cgccccacgg gtcgttgcct gcgggagcgc cgcgcctcat
      841 ccacaccgag cgcaccacgt tccagatgaa cagcagcgtg gccagggcga tgatcatcgc
      901 cccgatgctg atcagcacgt tcaccccgct ccatccggtc tcggccgggt agtcggcgat
      961 gcgccgtgcc atgccgtcca gccccagctg gtgcatcggg aagaaggtga ggttgaaccc
     1021 gatgaccagc agccagaagt ggaccttgcc caggccctcg ctcagcatgc gaccgctcat
     1081 cttgggtatc cagaagtaga aggcggcgaa ggcggcgaag gccgagccgc cgaacagcac
     1141 gtagtgcatg tgtgccacca cgtagtagct gtcgtgcacc tggtagtcga tgggtggtga
     1201 ggccaggaag accccggtga ttccgccggc caggaactgg aagaggaagc cgaacacgaa
     1261 gagcatgggc gtgctgaagc ggatgcgccc gcgccacatg gtcgcgatcc aggtaaagaa
     1321 cttgacgccg gtggggatgg cgatcaggaa ggaggtgacc gcgaagaaga cgttgctgac
     1381 cgcaccggtg gtgaacatgt ggtgagccca cacgctgaag ctgtaggcac cgatgagcag
     1441 cgtggcgaac accagcgccc ggtagccgaa gatgcgcttc gagctgaaga ccggcaccac
     1501 ctcgctcacc acaccgaaga agggcagggc catgatgtac acctcgggat gcccgaagaa
     1561 ccagaagagg tgctgccaca ggaccgagct gccgcccgcc gccgggtcga agaagacggc
     1621 accggtcgtc cgttcgatga agagcatgac cagcgccgcg gtgagcaccg ggaaggtgag
     1681 gatgatgagc agcgcggtga ccaggatgtt ccaggtgaag atcgggatgc ggaacatggt
     1741 catgcccggc gcgcgcatgg tgaacaccgt ggcgatgaag ttgaccgcgc ccaggattcc
     1801 ggacgtcccc gacaatatga ggcccatcac ccacaggtcc ccgccggcgc cgggggagta
     1861 ggtgtcggtg gagaggggcg ggtagccgta ccagccgaac gaggccgccc cgctgacggt
     1921 caggaagccg gagaaggcca ccagcccgcc cagcaggaag agccagtagg tcagggcgtt
     1981 cagccgcggg aaggcgacgt cgggcgcccc gatctgaagc ggcagcaggt agttcgcgaa
     2041 accgatgccc agcggcacga tggcaaagaa gatcatcccg gtgccgtgca tggtgaacag
     2101 ctggttgtag gtggcctcgt ccaccacctg cagcccgggg aaggacagct cggcgcgcat
     2161 gatcagcccc agcacgccgc ccagccccag gaagccgatg gcggtcacca ggtacatgat
     2221 cccgatgcgc ttgtggtcga ccgtggtcag gtagtcgacg aaggccgatc gttggggccg
     2281 gacgtgggcg accgtgctca cgagccggcc tcaccgccag atgcgagcca ggtgtcgaac
     2341 cggtcggccg ggatggcgtc gatgctgaac agcatgcggt cgtggagcag cccgcagaac
     2401 tcgccgcaca cgccgcggta ggtgccctcc tcctcgatgg tcagctcgat gcggttcgtc
     2461 ttgtcgggca gcacgtcgcg cttgatcagg aactcgggga cccagaagct gtggatcacg
     2521 tccggcgact cgaggtcgaa ggcgatccgc cgccccaccg gcaggaccag cacgggtggc
     2581 tcgccggtga tgccggtaat cacggtgtcg gtgccctcgt aggcgaaccg ccactgccac
     2641 tggaagccgg tgaccgtcac ggtgaggtcc gggtcttcgg tgcgccggtc gacctcggac
     2701 agcacctggg cggtcaggat gatgagaccg atgaccagca gggtgggcag cgcccaccac
     2761 agcagctcga gcctgatgtt ctcgtgcgtg tccgaggggc ggcgttcgtc gtcggtggcg
     2821 cgatagcgaa cgatgctcca gccgatcagt ccggcgacca ccacgaacac gatcgcggcg
     2881 gcgaccagga agatgttgta gagcgtgccg acgcgagcgc cttcggtggt gaccggctcg
     2941 ggtacgcatc cggccaggat ggcggccatg accggaaggg cgatcgcgcg acagactgcg
     3001 gcgaacgtcc gacccatccg cggccatgct aacccggcag ccgccgcggg gccaatgaca
     3061 ggtgttgagg ggaggtttcg gtggtgcgtg gcgatcctac gtatgatcag tgcagccatg
     3121 gtgacctacg acgagtcgac cgccctgctg gtggtcgacg tgcagaacga cttcgcggac
     3181 cccggcggca gcctctacgt gcgcggcggg gaggagctca tcccacgcat caacgacgag
     3241 gtgcaacggg cggtggccgc cggcgcgctg gtggtctaca cccaggactg gcatcccgag
     3301 tcgacgccgc acttcgccaa ggacggcggg gtctggccgg tgcactgcgt gatggacacg
     3361 tggggtgccg agctgcaccc gcagctggtg gttgccgggg agcggatccg gaagggcacc
     3421 aacggggagg atggctactc cggcttcacc atgcgcgacc cggtgagcgg cgagcaggtg
     3481 cccaccccgc tggacggcat gctgcgggag caaggtattc gctcggtggt ggtcgccggc
     3541 ctggcaaccg actactgcgt gtgctcgacg gtcatcgacg cgcagcggct ggggtaccgc
     3601 ggagtggtgc tgacggaggc ctcggcggcg gtcgacctcc agccgggcga tggcgagcgg
     3661 cgcctccagg aggcggcggc ggcgggcgcc gagctggtct gatgcggcga gcggcgtacg
     3721 ccctcatcgc attggtggga ttgacgctgc tggtggccgc cccggcccgc gctggcgacg
     3781 gggacgtgca cgtcatcacc atcgaccggg acatcaaccc ggtatcggct cggtacctga
     3841 tccggcagct ggacgaggcc gtggaggagg gcgccgccgc ggtcctggtg gagatcaaca
     3901 ccccgggcgg cctgctggac gcaacccagg acatcaccac cgccatgctc ggcgcgcagg
     3961 tgccggtgat cgcctacgtc accccgccgg gcacccgcgc cgcctcggct ggcgtgttcg
     4021 tgaccatgtc ggcgcacgtg gccgccatgg cgcccagcac gcgcatgggc gcggccaccc
     4081 cggtcagcag tgagggtggc gacatccctg aggatttgcg caacaagatc atcgccgaca
     4141 ccgtggagta cgcccggtcg ctggccgaag cgcgcggccg caatgccgac tgggcggagt
     4201 cggcggtggt ggacgcggac gtggtcggcg ccgaggaggc cgtggagctc gacgtggtcg
     4261 atttgatcgc cccggaccgg cgagccctcc tggaggagat cgacggtcgc gaggtgaccc
     4321 tgggcgacgg ctcgacggtc acgctccgga cggcggacgc tcccgcggtc gagcgcgaga
     4381 tgtcgccctt cgagcagctg ctgatggtgc tcgccaaccc gaacgtggcc gtgctgctgc
     4441 tcagcatcgg ttccatcggc atttacttcg agctcgccaa cccgggtacc ttcttcccgg
     4501 gcatcaccgg cgccatcgcg ctgatcctgg gcctcttcag cctcgggtca ctgcccatca
     4561 actacgcggg cctggcgctg ctgctcctcg gcctgacgct gctcggcgcc gagatctggg
     4621 tggcgtccgg cggcatcctg ggcatcggcg gcggcatcgc cttcctgctc ggatccttca
     4681 tcctgatcga tgacacccgc gccccgctgc tcgaggtgag ccgtccgctg atcctcggca
     4741 tcaccctggt gctagtcggg ttcgtcgtct ttgccctgca ggcggtgatg cgagcccgtc
     4801 atcggccggc ctacatcggg ggaggcgacc tcgtcgggcg ggaggcatcg gtccgtgggc
     4861 cagatgccgt ctacatcgcc ggcgagctgt ggtcggcacg acccgcttcg ggtgagggct
     4921 cgctgactcc cggacggcgc gttcgcgtgg tggaccggga gggactgcac ctgctggtcg
     4981 aggaactacc ggagaccgat cccgacgtcc gcgaggcgga cgccggccaa cctactgagg
     5041 agggtaccta ggtggaagag ctgctccaga tcctgcccgt catcgtggtg gccctggtgg
     5101 ccatcgcctg gatcgtgggc gcggccgtca aggtcgtcca ggaatacgag cgcggggtca
     5161 tcttccggct cggccgcctg atcggcgcca aggggccggg cctgttcttc atcatcccga
     5221 tcatcgaccg gatggtgaag atcgacctgc gaacggtgac gctcgacatc cccagccagg
     5281 aggcgatcac ccgggacaac gtgaccgtcc gggtcaacgc ggtgtgctac ttccgcgtgg
     5341 tggatcccga ggcggcggtt gtgcgcgtcg agaacttcct gctggccacc acccagatcg
     5401 cacagacgac aatgcgttcc gtcctcggcc agtccgagct ggacgagctg ctcagcaagc
     5461 gcgaggagat caacgcccgg ctgcagcaga tcatcgacga gcagaccgag ccatggggcg
     5521 tcaaggtcag cgtggtcgag gtcaaggacg tggagctgcc gcagaccatg cagcgcgcca
     5581 tggctcgcca ggccgaggcc gagcgcgaca agcgagccaa ggtgatccac gccgagggcg
     5641 agttcatggc cgcccagcag ctgtccgagg cggcgaacat catcaactcc gcccccagcg
     5701 cactgcagct gcgctacctg cagacgctgt cggacatcag ctcggaccag agccgcacca
     5761 tcatcttccc gctgcccatg gacctgatca gtgccttcat cggcacccgg gccgatggcg
     5821 agtcgggcgg cggtggtggt gggtcgaact cgccggtcaa gcccgtgacg ccgaccaccg
     5881 gggggcaggc cacggagtag gcgcagaacc ggtcggatcg tgctccgcgc gggtcccatc
     5941 gggcaggttt gtagcgcggc ttgcacccgg gaggatgggg ggtatactcg cgcgagtcgg
     6001 gccgcaaaag gagcggcgcc agcaccgatt gcactcccct cccaccacgt ctcgtcgtcg
     6061 gctcacgggg tagcggcaag acccgtgacc ggcgttgatc ccgaggcgga agccgacgtg
     6121 gtaaccatct tctcagggag gggtccttcg tggcctacac cgatcaggtg ctcacctgtg
     6181 cggactgcgg gatcgacttc gtcttctccg catccgagca ggagttcttc gcgcagaagg
     6241 gattcacctc tgcgcccaag cgctgtccct cgtgccgggc gcagcgtcgt gcctcgggcg
     6301 gcggctcgag ctacggaaac ggcggcggct acggcggcga caccggctac agccgtggcc
     6361 cgcgcgagat gttcgacgcg gtctgcgccc gctgcggcaa ggacacccag gtgcccttcc
     6421 gtcccaccgg cgcacggccc gtctactgca gcgactgctt ccggttggta cgcggttagg
     6481 tcgcgcccag cgcgacggat cgctccttcg cggcgtcccg gctcgaccgg ggcgccgctt
     6541 cgtcgtatgc gccagtcgct tcccaacgcg cgtcttcttg gtcacatgct cgggacgtgg
     6601 gcattacggc cgaacttcgt accttgaacc tgggctcgtg tgcacgccgc gagccttaga
     6661 cgagcttccc acgctcgcgg gtcggtgggt gacgctcatc caggtcggct aaggcgtacc
     6721 tggtgaacct ctgaccgaga aaaacggcca agtgcacaga taaggctcac cagccgagcc
     6781 gatggccaag accggcggga tggaagccgc gcgtcgtacc gcctttaccc ggtcgcccgg
     6841 cgagcaggcg cggacagggc tcgtatcatg gtccgtaacg acctgcatgg gcagagaact
     6901 tgcggacata cgtgacgaca tgaccgacct cgagcagtgg cgcgagctgg gacagcagct
     6961 gcgcgtcgac tccatccgat ccagctcggc cgccgggtcg ggccacccca cctcctccat
     7021 gtccgccgcg gacctcatgg cggtgctcat ggcccgccac ctgcgctacg acttcgccgc
     7081 cccggacaac ccaaacaacg accacctcat cttcagcaag ggccacgcct cgccgctgct
     7141 gtacgcgatg tacaaggccg ccggtgccct gtcggacgag gagatgctca ccttccgtga
     7201 gctgggcagc cgggtggagg gccaccccac gccggtgctg ccctgggtgg acgtggccac
     7261 cgggtcgctg ggccaggggc tgcccatcgc ggtcggtgtg gcctacgccg ggcgcaacct
     7321 ggaccgcctc ccgtaccggg tgtggtgcct gaccggggac tctgagatgg ccgaggggtc
     7381 gatgtgggag gcgttcgagg cggccggctt ccacggtctc ggcaacctga cggccatcat
     7441 cgacgtcaac cgccttgggc agaccggcga gacaatgcac ggctgggacc tggatgccta
     7501 cgcccgtcgc gcacgggcat ttggctggca caccgtcgag atcgacggcc acgacgtcga
     7561 agccatcgac cgcgcctatg ccgaagccgc aggaattgag gatcggccga cagcaatcat
     7621 cgctcgcacc gagaagggcc atggcgtgga ggccgtggcc aaccagaacg gcgcgcacgg
     7681 caagccggtg agcgacgaag cagccgcgat tgaagagctg gggggcccgc gccagctgaa
     7741 catctccgtg accccgcccg agggcacggg agagccgcat cggttcccca cccagggaga
     7801 gctgaagctg ccgtcctacg agccgggcac caaggaggcg acgcgcaagg cgtacggcga
     7861 cgcgctgctg gcgctgggac aggcgcgcgg cgacgtagtg gccatcgacg gcgaggtggg
     7921 caactcgacg tactcggagc tcttcgccaa ggagctgccc gagcgcttcg tgcaggccta
     7981 catcgccgag cagatgatgc tcagctacgc ggtcggcttc caggtgcggg gctggcgtcc
     8041 attcgccgcc acctttgccg ccttcctgtc acgtgcctac gacttcgtgc gcatggcggc
     8101 ggtgagccgc gccaacatcg tgctggccgg gtcgcacgcc ggcgtcagca tcggtgagga
     8161 cggaccgtcg cagatggcgc tggaggacct tgccagcctg cgcgcggtgc acggcagcac
     8221 ggtgctctac ccgtcggacg ccaaccaggc ggccgacctg ctggcgcaga tggcggaccg
     8281 caccggcatc gtcttcatgc gcaccacccg cgagaagacg ccggtcatct accagcccgg
     8341 ggagcgcttc cccataggcg gcagccgcct ggtgcgcagg accgatgacg accaggtcac
     8401 gctggtcggc gcgggcatca ccctgcacga ggccatcaag gccgcggacg cgctcgcctc
     8461 ggatggcatc agtgcccggg tcattgacct gtactcggtc aagcccttgg acgccgagac
     8521 gctgcgcgcg gccgccagcc agacggcggg catcctcacc gtcgaggatc actacccgga
     8581 gggcggcatc ggcgacgcgg tgctcgacgc cctggccggc gacgggctgg gcgtgccggt
     8641 gcgcaagctg gcggtgcgca tcatgcccgg ctcggccaag ccggaggagc agctggccgc
     8701 cgccggcatc gacgccaagc acatcgcgga tgcggcgcgc gtgcggacca gctcgcgcgc
     8761 cg
//
LOCUS       DATACA010000240         3692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_673878, whole genome shotgun sequence.
ACCESSION   DATACA010000240 DATACA010000000
VERSION     DATACA010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673878"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VHK28_05015"
     CDS             complement(<1..686)
                     /locus_tag="VHK28_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX2141624.1"
                     /translation="MTRQFKPVSKNLDVPALEREVIERWRQRGTMARYLARNDAAEKT
                     FSFIDGPITANNPMGVHHAWGRTYKDLYQRYHTMLGERQRYQNGFDCQGLWIEVEVER
                     QLGFNNKQQIEEFGIDRFVELCKERVRTFAGIQTEQSKRLGYWMDWANSYYTNSDENN
                     YTIWAFLAECHRRGLLYRGHDAMPWCTRCGTGISNMEAAEGYAEEKHLSLTLRLPITS
                     EGHADEDLLV"
     gene            complement(1046..2155)
                     /locus_tag="VHK28_05020"
     CDS             complement(1046..2155)
                     /locus_tag="VHK28_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141625.1"
                     /translation="MRHRVPPIARIRLARSLPRLLAWPALVAATGAMAIASWFLGSFG
                     SGAGGVVFAVVGVALVLVGLVTAVLLLSIRLEVHESAVRLSWVGGERTYPLVRGPVTR
                     VRLRGPNASKLRSRVGFFGWAVGSARLRDEEEIHVVRLARTATAILVPTERGRLAIAA
                     ADEDALLDALSRAARARQRLDELTDGSQTPEIRTSEADRATEPEPRPPDVVAVEVQPK
                     VLTGIERALLERGLAQEREEAAAALAAEAARAADMSGSTPSDGLEAADGPAAKPLQQR
                     RRRLTLRRPQPSAVFILLPLIGSGVAWGLGLLTDSYPEPGTDLGRLTALALVLAGPAT
                     SVGAIMARAWWPRLVGLVVAGGLCASAFIARALIG"
     gene            complement(2159..>3692)
                     /locus_tag="VHK28_05025"
     CDS             complement(2159..>3692)
                     /locus_tag="VHK28_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141626.1"
                     /translation="AARPGRGDAMTTVDSQDRYQWLGRVLEMVPGTISWAVLILPIWL
                     SFSYPWLVAYFVLSFDFYWLCRALWFAAAVIVAYRRIRRVLAVDWGARLGELAPTADA
                     PAADDLVHLALIPTYTEPLEKLQDTVRALAAAEWPRERKICAIITRETDAGGISNVKA
                     LRAEFGDAFAEFIHILDPLEPGIVVGKSSAMAWGGRFLYRLLVRERGMDPRRILVTDL
                     DADYRVHPQYFTYLAWTHLTDPNRETQLYQPIPYFHNNIWEAPLLQRLFAAVLTQLQM
                     WRSVLPEKLQSFGSYSTTLHLVHDVGYWATDAIPEDSRFYWKSYFRYGDRFRAVPLFI
                     PIYGDAVRARGYWRSMAEQYFQARRWAWGVTDIPYVVQNAVRSPHIPFWSRFWRVINL
                     FGEHINWAIAPFVVMFGATVPLLINPEFAETTLGQNLPLFASTMLTMALVSLLVLVVI
                     EHRIVPPRPAHWGRMQRIISYVQWLGLPFVGIFFSTLPALDAQTRLLTGRYLEYRVTE
                     KA"
BASE COUNT          591 a         1266 c         1292 g          543 t
ORIGIN      
        1 cagacgagca gatcctcgtc ggcatggccc tcgctggtga tcggcagacg gagggtgagc
       61 gaaaggtgct tctcctcggc gtacccctcc gcagcctcca tgttgctgat gccggtgccg
      121 cagcgcgtgc accacggcat cgcgtcgtgg ccgcggtaca ggagcccgcg acggtggcac
      181 tcggccagga acgcccagat cgtgtagttg ttctcgtcgg agttcgtgta gtagctgttg
      241 gcccagtcca tccagtagcc gaggcgcttg ctctgctcgg tctggatgcc ggcgaacgtc
      301 cggacccgtt ccttgcagag ctcgacgaat cggtcgatgc cgaactcctc gatctgctgc
      361 ttgttgttga agccgagctg ccgctcgacc tcgacctcga tccagaggcc ctggcagtcg
      421 aagccgttct ggtaccgctg ccgctcaccg agcatggtgt ggtagcgctg gtacaggtcc
      481 ttgtaggtgc ggccccaggc gtggtgcacg cccatcgggt tgttggccgt gatcggcccg
      541 tcgatgaagc tgaacgtctt ctccgccgcg tcgttgcgcg cgaggtagcg ggccatcgtc
      601 ccgcgctgcc gccagcgctc gatgacctcc cgctcgagcg cgggaacatc cagattcttg
      661 gaaaccggct tgaactgcct ggtcatcgac gctccataca cgacaaaccc gccccggaac
      721 tccgggacgg gaatcactcc cgcggtacca cccgcgcttg gccgatgcgc gcccgcagcg
      781 cggccggccc actcaaccgc cacgcgttcg acgcgcgaac ccatggcggc gccgctgcta
      841 tcgggtggcg cacccgggag gcgttaccca cggcgcgtac cgcttggcgc ctccggctcc
      901 gaagggatga cgtgccgcct ccggatcgcc cgctcgcacc gtccgggctc gctcctggtc
      961 cgggtcggac ggccgccgtg gctccgtcgt cgcctctcgt tcggttggtg ggccgatcgt
     1021 cgcagccgac cgatgcggtg tcaattcagc cgatcagcgc ccgggcgatg aaggcggatg
     1081 cgcacaggcc accggccacc accaatccca ccaggcgggg ccaccacgcg cgggccatga
     1141 tggcgccgac cgacgtggcc gggccagcca gcaccagcgc cagcgccgtg agtcgcccga
     1201 ggtccgtgcc gggttctgga tagctgtcgg tcaagagacc cagtccccac gccaccccgg
     1261 agccgatcag cggcagcagg atgaacacgg cggacggttg cgggcgcctg agcgtcagcc
     1321 gccgacgtcg ttgctgtaag ggcttggcgg caggcccgtc cgccgcctcg agcccatccg
     1381 acggcgtact ccctgacatg tcagcagccc gagcggcttc cgcagccagc gcggcggcgg
     1441 cctcctcccg ctcctgcgcc aggccgcgct cgagcagcgc gcgctcgatc ccggtgagca
     1501 ccttcggctg gacctcgacg gccacgacgt ccgggggtcg gggctcgggt tcggtggctc
     1561 gatcggcctc ggacgtcctg atctccgggg tctggcttcc gtcggtcagc tcgtcgagcc
     1621 gctgccgcgc ccgcgccgct cgcgacaggg cgtcaagcag ggcgtcctcg tcggcggccg
     1681 cgatggcgag gcgcccgcgc tccgtcggca cgaggatggc ggtcgccgtg cgagcgagcc
     1741 gcacgacgtg gatctcctcc tcgtcacgga ggcgcgcgga gccgacggcc cagccgaaga
     1801 agccgacgcg cgagcggagc ttcgaggcgt tgggaccgcg caggcgaacg cgggtgacgg
     1861 ggccgcgcac gagcgggtag gttcgctcac cgccgaccca cgagagccgc acggccgatt
     1921 cgtgcacctc gaggcggatc gagaggagca ggaccgcggt gacgaggccg acgagcacaa
     1981 gggccacgcc gacgaccgcg aagacgaccc ctcccgcgcc cgagccgaag gagccgagga
     2041 accagctggc gatcgccatg gcgccggtcg ccgccaccag ggccggccag gccagcaggc
     2101 gcggcaggga tctggccagg cggatgcgcg cgatgggcgg gacccggtga cgcatcggtc
     2161 aggccttctc cgtgacccgg tactcgaggt atcggcccgt cagcagacga gtctgcgcgt
     2221 cgagcgcagg cagcgtgctg aagaagatgc cgacgaaggg caggcccaac cactgcacgt
     2281 agctgatgat gcgctgcatc cttccccagt gcgccgggcg cggcggcacg atgcgatgct
     2341 cgatcacgac cagcacgagc aggctgacga gggccatcgt cagcatcgtg cttgcgaaca
     2401 gcgggagatt ctggccgagc gtcgtctcgg cgaactccgg gttgatcagg agtggcacgg
     2461 tggcgccgaa catcaccacg aacggggcga tcgcccagtt gatgtgctcg ccgaacaggt
     2521 tgatgacccg ccagaagcgg gaccagaacg ggatgtgagg gctccgcacc gcgttctgga
     2581 cgacgtacgg gatgtccgtc acgccccatg cccaccggcg ggcctggaaa tactgctccg
     2641 ccatgctgcg ccagtagccc cgggcacgga cggcgtcgcc gtagatcggg atgaacaggg
     2701 gcaccgcgcg gaatcggtcg ccgtagcgga aatagctctt ccagtagaag cggctgtcct
     2761 ccgggatcgc atcggtcgcc cagtagccca cgtcatggac gagatgcagc gtcgtcgagt
     2821 acgaaccgaa gctctgcagc ttctcgggca gcacgctgcg ccacatctgc agctgcgtca
     2881 ggacggcggc gaacagccgc tggagcagcg gcgcctccca gatgttgttg tggaagtacg
     2941 ggatcggctg gtagagctgc gtctcgcggt tcgggtcggt caggtgggtc cacgccaggt
     3001 acgtgaaata ttgcggatgc acccggtagt cggcatccag gtcggtgacc aggatccggc
     3061 gtggatccat gccgcgctcg cggaccagga gccgatacag gaagcggccg ccccacgcca
     3121 tggcgctcga cttgccgacc acgatccccg gctcgagggg gtcgaggatg tggatgaact
     3181 cggcgaaggc gtcaccgaac tcggcccgca gggccttcac gttcgagatg ccgcctgcgt
     3241 cggtctcgcg agtgatgatc gcgcagatct tccgctctct gggccactcg gcggcggcca
     3301 gggcacgaac ggtgtcctgc agcttctcga gcggctcggt gtaggtcgga atcagcgcca
     3361 ggtgcacgag gtcatcggcg gcgggggcgt cagcggtcgg tgcgagctct ccgagacgcg
     3421 cgccccagtc gaccgccagc acccgccgga tgcgccgata ggccacgatg accgcagcgg
     3481 cgaaccacag ggcgcggcac agccagtaga agtcgaagct gagcacgaag tacgcgacga
     3541 gccacgggta gctgaagctc agccagatcg ggaggatgag gaccgcccac gagatggtgc
     3601 ccggcaccat ctccagcacg cggccgagcc actgatagcg gtcctggctg tcgaccgtgg
     3661 tcattgcgtc gcctcgtccc ggccgcgcgg cc
//
LOCUS       DATACA010000241         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_996038, whole genome shotgun sequence.
ACCESSION   DATACA010000241 DATACA010000000
VERSION     DATACA010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996038"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..763
                     /locus_tag="VHK28_05030"
     CDS             146..763
                     /locus_tag="VHK28_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX2141627.1"
                     /translation="MAYLSMGPEQAAEALRRALAMGGEEAYLVTDASLAGADEWATAK
                     VLAAALDKLAPDVALMGMASDDARGSLVPGAVAAIRKLPLLSYGSELSLADGSARIRR
                     LSAAGYDILEAPLPVVATVTDQVGEPRYASLKGIMAAKRKPLETWSLGDIGLSADALG
                     GRPALTRVVEARPPEAKPPTERIENLGADEAAVNVADWLAARKLV"
     gene            763..1746
                     /locus_tag="VHK28_05035"
     CDS             763..1746
                     /locus_tag="VHK28_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX2141628.1"
                     /translation="MSNDVFFLAEFAEGSPQRSALELAAGAADLAQQSGGSAVALAYG
                     PGARDGSPGLGGVGASRVLVLGDGADPAITFAEAAAAAVRDAQPLALLAPATPNGRDL
                     AAALVGLLDVPAFGPVRAVRVEDGKLRTEQATLQNTVITVNEPAEAASRPSVVLVLAN
                     TFTPREGGSGTAEVADAPSTDAGAFGKVRVAESHPAEAAVVNLEEATVIVAGGRGVGS
                     AEGFEPLHELAAALGGAVGASRAAADAGWIPYQLQIGQTGKVVKPSLYVGVGISGAIQ
                     HRVGMQTAEHVVAINKDPDAPIGEFADLFVVGDLFAIIPKLTEEIRRRKGT"
BASE COUNT          260 a          656 c          692 g          259 t
ORIGIN      
        1 gccccggcca gcgaggcatc ggtcagcagg aaggcctcct caccacccat ggccagagcg
       61 cgacgcagtg cctcccctgc ctgctcggga cccatggaca ggtaggccac gctttcggcg
      121 gcacccgact cctgcagccg aaagcgtggc ctacctgtcg atgggtcccg agcaggcagc
      181 ggaggccctg cgtcgggcac tggccatggg tggtgaggag gcctacctgg tgaccgatgc
      241 ctccctggcc ggcgccgatg aatgggccac cgccaaggtc ctggccgccg cgctggacaa
      301 gctggccccc gacgtggccc tgatgggcat ggcgtccgat gacgctcgcg gctcgctggt
      361 ccccggagcg gtggccgcca tccgcaagct gccgctgctg tcctatggct ccgagctgtc
      421 gctggctgac ggatcggccc gcatccggcg cctgtcggcg gccggctacg acatcctgga
      481 ggcaccgctg ccggtcgtgg ccactgtcac cgaccaggtt ggcgagccac gctacgcaag
      541 cctgaagggg atcatggccg ccaagcgcaa gccgctggag acgtggtcgc tgggcgacat
      601 cggtctttcg gccgacgcgc tcggtggacg gccggcgctg acccgggtgg tggaggcgcg
      661 accgcccgag gccaagccgc cgaccgaacg aatcgagaac ctgggtgccg atgaggccgc
      721 cgtcaacgtc gccgactggc tggcggcgag gaagctggtc tgatgagcaa cgacgtcttc
      781 ttcctggccg agttcgccga gggcagtccg cagcgcagcg ccctggagct ggcggccggt
      841 gccgccgacc tcgcgcagca gtcgggcggt tctgccgtgg cgctggccta cgggcctggt
      901 gcgcgggacg gctccccggg gctgggtggc gtcggtgcca gccgggtgct ggtgctcggc
      961 gatggggctg atcccgccat caccttcgcc gaggctgcgg cggccgccgt tcgcgatgcg
     1021 cagccgctgg ccctgctggc accggccacg ccgaatggtc gcgacctggc cgccgcgctg
     1081 gtcgggctgc tggacgtgcc ggccttcggg ccagtgcgtg ccgtgcgcgt cgaggacggg
     1141 aagctgcgca ccgagcaggc cacgctgcag aacacggtca tcaccgtcaa cgaaccggcc
     1201 gaggccgcct cccgcccgtc ggtggtgctg gtcctggcca acaccttcac cccgcgggag
     1261 ggcggcagcg ggacggcgga ggtggccgat gcgccatcga ccgacgctgg agcgttcggc
     1321 aaggtccgag tggccgaatc gcacccggcc gaggccgccg tggtgaacct ggaggaggcc
     1381 accgtcatcg tggccggcgg gcggggcgtg gggtcggccg aaggcttcga gccgctccac
     1441 gagctggccg ccgcgctggg cggggcggtg ggcgccagcc gggccgcggc cgacgccggc
     1501 tggatcccgt accagctgca gatcggccag accggcaagg tcgtcaagcc atcgctctac
     1561 gtcggggtgg ggatcagcgg cgccatccag caccgcgtcg ggatgcagac cgccgagcac
     1621 gtggtggcga tcaacaagga tccggacgca cccatcggtg aatttgccga cctcttcgtg
     1681 gtcggggacc tgttcgccat catccccaag ctgaccgaag agatcaggcg tcgaaaggga
     1741 acgtgatgcc caagccctcc atcctcggca cccttgccct gctggccctg ccggcgagct
     1801 tcctgctggg ccgcggcggc aagcgccgcc ggccgctggg ctacggcaag gagctgcgcc
     1861 agtaccg
//
LOCUS       DATACA010000242         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1317191, whole genome shotgun sequence.
ACCESSION   DATACA010000242 DATACA010000000
VERSION     DATACA010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317191"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..821)
                     /gene="purQ"
                     /locus_tag="VHK28_05040"
     CDS             complement(87..821)
                     /gene="purQ"
                     /locus_tag="VHK28_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2141629.1"
                     /translation="MSRGPRVAVTVFPGTWSERDFADLAEPLGWDVRLVWHAEASLGD
                     ADAVVLPGGFAHGDHLRTGAIARFSPVMGAVEAFAASGGPVLGSCNGFQILCEAGLLP
                     GVLLRNEHLEFRCDWQDLRVERSAGAWLAADEVQPGDVIRLPISHGEGRYHADAPVLD
                     ELEAMGRVVLRYADAAGRVTPDANPNGSQRGIAGIVNDRGNVLGLMPHPERAADPEAG
                     GTDGLRLLRSLDRWLAAQPAAAERRL"
     gene            complement(818..1099)
                     /gene="purS"
                     /locus_tag="VHK28_05045"
     CDS             complement(818..1099)
                     /gene="purS"
                     /locus_tag="VHK28_05045"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2141630.1"
                     /translation="MRWLADVHVRLRPGIADPEGQTIAGGLHALGFSSVQEVRAGKLL
                     RLTFEASDHGAAEQAVAEMCRRLLANPVMETAEWELRADVDQATEGISR"
     gene            complement(1101..2000)
                     /locus_tag="VHK28_05050"
     CDS             complement(1101..2000)
                     /locus_tag="VHK28_05050"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2141631.1"
                     /translation="MTLLRSRIEGLPLIHRGKVRETYAVGDDRLLLVATDRLSAFDVV
                     FDQPIPDKGAVLTRLSAWWFRRLEALGPTHFISTDASDLPEAARTPELAGRVTLARRA
                     RRIDAECVVRGYLAGSGWAEYRRSGEVGGHRLPAGLQEADRLPEPIFTPSTKAEVGHD
                     ENITRAQLAEMVGDELAAELEERSLALYRAGAERAAEVGLILADTKFEFGWIDGRVAV
                     IDELLTPDSSRFWDESQYAPGSSPPSFDKQYVRDFVAGSGWDKEPPAPDLPAEVIDGT
                     RSRYVTAYERLAGERWSPTEASV"
     gene            complement(1997..2506)
                     /locus_tag="VHK28_05055"
     CDS             complement(1997..2506)
                     /locus_tag="VHK28_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008564509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX2141632.1"
                     /translation="MTPEISTGLQIELAMRLTLGLVLGALIGFERELHRQPAGFRTHS
                     LVSLGAALFTIVSAYGFTGDLVDPTRIAAQIVSGIGFIGAGTILQHRGSIRGLTTAAS
                     LWSVAAIGTAAGAGMVVMAVIGTLLILVVLAVLDRIEAWVTRRLNLPPEGKTQTPASS
                     DDGRTEDMP"
     gene            complement(2517..>3164)
                     /locus_tag="VHK28_05060"
     CDS             complement(2517..>3164)
                     /locus_tag="VHK28_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141633.1"
                     /translation="PSASRPRAAGATMAGLASLLVALLPTAAAAAAVGIGVAIAAPGA
                     FTSPDLETPILLRLASDVWPLLLVLAAAMVLSQALGAVAFRRATAHLPQPLAAAITGS
                     VADLLRRPASRLGTAAAGMLVDGVLLILTWAVLRVLWAPIAADLGVGRLGGPDTLLLL
                     LGFVAVWLVLLLAAGAWHVAMSAWWALELARDGSEARQVPHRIEDAGTTLDQPTP"
BASE COUNT          474 a         1177 c         1119 g          394 t
ORIGIN      
        1 gtggcgttga catgtcgctc gggtcccgtt cgtcgtgggt catcggacgg tcagcctcca
       61 atcgggccgg gatgcgacgg cctgcatcat agccgacgct cggcggcggc cggctgggcg
      121 gccagccatc ggtccaggga gcggagcagg cgaagcccat cggtcccgcc cgcctcggga
      181 tcggccgcgc gctccggatg cggcatcagc ccgagcacgt tgccacgatc gttgacgatg
      241 ccggcgatgc ctcgctgcga gccgttgggg ttggcgtcgg gcgtcacccg cccggcggcg
      301 tccgcgtagc gcaggacgac ccgccccatc gcctccagct catccagcac cggggcgtcg
      361 gcgtggtagc ggccctcccc gtgggagatg ggcaggcgga tcacgtcacc cggctgcacc
      421 tcgtcggccg ccagccaggc accggcggag cgctccacgc gcaggtcctg ccagtcacag
      481 cggaactcga ggtgctcgtt gcgcagcagg acgcctggca ggagcccggc ctcgcacagg
      541 atctggaagc cgttgcacga gccgagcacc gggccgccgc tggccgcgaa cgcctccacc
      601 gcccccataa ccgggctgaa gcgggcgatc gcgcccgtcc gcaggtggtc accgtgcgcg
      661 aacccgccgg gcagcacgac cgcatccgca tcgcccagcg acgcctcggc atgccacacc
      721 agccggacgt cccagcccag cggctccgca aggtcggcga agtcgcgctc gctccaggtc
      781 cccggaaaga ccgtcaccgc cacgcgcggc ccgcggctca tcggctgatc ccctcggtgg
      841 cctggtcgac gtcggcgcgc agctcccact cggcggtctc catcaccggg ttggccagca
      901 gccggcggca catctcggcc accgcctgct cggcggcgcc gtgatcggac gcctcgaagg
      961 tcaggcgcag cagcttgccg gcacggacct cctggaccga gctgaagccc agggcgtgca
     1021 acccaccggc gatggtctga ccctccggat cggcgatccc cggacgcagc cggacgtgga
     1081 cgtccgccag ccagcgcacg ctagaccgat gcctccgtgg ggctccaccg ctccccggcc
     1141 aggcgctcgt aggcggtgac gtagcggctc cgcgtcccgt cgatcacctc agccggcagg
     1201 tccggcgccg gcggctcctt gtcccagccc gatcccgcca cgaagtcacg cacgtactgc
     1261 ttgtcgaagc tgggcggcga cgatcccggc gcgtactggg actcgtccca gaaacggctc
     1321 gagtcgggcg tcagcagctc gtcgatgacc gccacccggc catcgatcca gccgaactcg
     1381 aacttggtgt cggccaggat cagccccacc tcggccgccc gctcggcccc cgcgcggtag
     1441 agcgccaggg aacgttcctc cagctccgcg gccagctcgt caccgaccat ctcggccagc
     1501 tgggcgcggg tgatgttctc gtcgtgaccg acctcggcct tggtcgaggg cgtgaagatc
     1561 ggctccggaa ggcggtccgc ctcctgcagg ccggcgggca ggcggtgacc gccgacctcg
     1621 ccgctgcgcc ggtactcggc ccacccggac ccggccaggt agccgcgcac cacgcactcg
     1681 gcgtcgatgc gccgcgcccg cctggccagc gtcacccgcc cggccagctc gggcgtgcgg
     1741 gcggcctcag gcaggtcgga ggcatcggtc gagatgaagt gggtgggccc cagcgcttcc
     1801 aggcgacgga accaccaggc cgagagccgc gtgaggaccg ctcccttgtc ggggatcggc
     1861 tggtcgaaga ccacgtcgaa ggcggagagc cgatcggtcg ccacaagcag cagccggtcg
     1921 tcgccgaccg cataggtctc gcgcaccttg ccgcggtgga tgaggggcag gccctcgatg
     1981 cggctgcgta gcagggtcat ggcatgtcct ccgttcgtcc gtcgtcggat gaagccggcg
     2041 tctgggtctt accctccggc ggcaggttga gacgcctggt cacccatgcc tcgatgcgat
     2101 cgagcacggc cagcaccacc aggatcaata gcgttccgat gaccgccatg accaccatgc
     2161 ctgcgccagc cgcggtccca atggcggcca ccgaccacag cgaggcggcg gtggtcaaac
     2221 cgcggatgct gccccggtgc tggaggatgg tcccggcgcc gatgaagccg atgcccgaga
     2281 cgatctgggc ggcgatccgc gtgggatcca ccaggtcgcc ggtgaagccg taggcggaca
     2341 cgatggtgaa gagggcggcc cccaacgaca ccaggctgtg ggtccggaag ccggccggct
     2401 ggcggtggag ctcgcgctcg aagccgatga gcgcgcccag gaccagcccg agcgtcagcc
     2461 gcatcgccag ctcgatctgg aggcctgttg agatctccgg cgtcatcggc gcctcctcag
     2521 ggggtcggct ggtcgagcgt ggtgccggcg tcctcgattc gatgaggcac ctgtcgggcc
     2581 tccgaaccgt cccgtgccag ctccagcgcc caccacgccg acatcgcgac gtgccacgcg
     2641 ccggcggcca gcagcagcac cagccagacc gcaacgaagc ctagcagcag caggagggtg
     2701 tccgggcccc ccagccgccc cacgccgaga tcggcagcga tgggcgccca cagtacccgc
     2761 agcactgccc aggtcaggat gagcaggacg ccgtccacca gcatgccggc cgcggcggtg
     2821 cccaaccgcg aggctggccg tcgcagcagg tcggcgacgg agccggtgat ggcagctgcc
     2881 aggggctgcg ggaggtgcgc cgtggcgcgc cggaaagcca ccgcacccag cgcctggctg
     2941 aggaccatgg cggcggccag caccagcagg agcggccaga catcggatgc caggcgcagg
     3001 agaatgggcg tctcgaggtc tggcgaggtg aatgcgcccg gcgccgcgat ggcgaccccg
     3061 atgcccacgg cggccgcggc ggctgccgtg ggcagcagcg cgaccagcag gctggccagg
     3121 cccgccatgg tggcgcccgc cgcccgaggc cgcgaggccg acgg
//
LOCUS       DATACA010000243         4808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1959618, whole genome shotgun sequence.
ACCESSION   DATACA010000243 DATACA010000000
VERSION     DATACA010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959618"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..654)
                     /gene="recR"
                     /locus_tag="VHK28_05065"
     CDS             complement(40..654)
                     /gene="recR"
                     /locus_tag="VHK28_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX2141634.1"
                     /translation="MSGLIAPVARLIDEFSRLPGVGRKTAQRLAYHVLRAPADEARDL
                     AAALISVKEDVAYCSVCCNITDRGVDPCAICADARRDPTRICVVEEPLDVLAIERTAE
                     FRGLYHVLHGAISPIDGIGPEQIRSRELVARVTEGSIGEVILATNPNLEGEATAMYLA
                     DLLQPHVPVVTRIARGLPVGGDLEYADDVTLIRSLQGRRQVERS"
     gene            complement(698..1045)
                     /locus_tag="VHK28_05070"
     CDS             complement(698..1045)
                     /locus_tag="VHK28_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX2141635.1"
                     /translation="MNIGQITKIAQQMQAQMAQAQEELKETTLETTSGGGAVRVVITG
                     AQEIRYIEIDPGAVDPEEVEMLQDLVMAAVNDAVTRSKDLERERMSAVAGGLGLPGMP
                     GMGGMGGPALPGR"
     gene            complement(1072..2778)
                     /gene="dnaX"
                     /locus_tag="VHK28_05075"
     CDS             complement(1072..2778)
                     /gene="dnaX"
                     /locus_tag="VHK28_05075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX2141636.1"
                     /translation="MYGTEAMSTSLPTDSPPAPTATPGGDQPADQPADQHKALYRRWR
                     SQRFAEVLGQDAIVTTLRRAVATDRLAHAYLFVGPRGTGKTSTARILAKAINCTNRGE
                     DGEPCDTCAACVSIREGRAMDVIEIDAASHGLVEDARDLVMRALTAPSELRRRVYIID
                     EVHMLSTHAFNALLKMVEEPPPHVVFVLATTDTHKVPATMISRTQRFDFRRLPTDAIA
                     GKLGRIVDAEEADAEPEALTLIARLADGGMRDAESLLDQVLAFAGERLTAEDVREAVG
                     LADDEAIVALVDAYVAGDAGAALDGIEGLVDRGRDMGQVAAQAEVEARRRLLAAAADP
                     QLARRLAHILRTVAEAAGAGAREGRARLALELLAVDGLAPTAEAPAVAVAAARPVAPK
                     PAPEVAKVERSGPPLAPKPAVQATSAPAARQAAAVEEQAAPEPEPVAATPGERPEVAE
                     LRARWGEVVQKAAPAIKPLLSDCRPVSVDGARLTLAFPEERAFMREKARTRAPAIEQL
                     LAQVLGGAWAIDCVASNVELEPLSVAQAVVADPSDADGQALLDGVLRITGGELVDVPE
                     VR"
     gene            complement(2848..3114)
                     /gene="ffs"
                     /locus_tag="VHK28_05080"
     ncRNA           complement(2848..3114)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VHK28_05080"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(3173..3260)
                     /locus_tag="VHK28_05085"
     tRNA            complement(3173..3260)
                     /locus_tag="VHK28_05085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3224..3226),aa:Ser,seq:gga)
     gene            complement(3310..3396)
                     /locus_tag="VHK28_05090"
     tRNA            complement(3310..3396)
                     /locus_tag="VHK28_05090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3360..3362),aa:Ser,seq:cga)
     gene            complement(3447..3890)
                     /locus_tag="VHK28_05095"
     CDS             complement(3447..3890)
                     /locus_tag="VHK28_05095"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2141637.1"
                     /translation="MNYELFMAEAIAEARRGSEEGEIPVGAVAVLDDAMIARDHDRMQ
                     LQRDPTAHAVLLTLQATARKLESRRLSEISIFATHEPCAMCVGALLEAQVGSLIYAAP
                     DSAKGAAGGAIDLARASGLPHQLSVISGVREAEVRRLANGSAVAK"
     gene            4014..4490
                     /locus_tag="VHK28_05100"
     CDS             4014..4490
                     /locus_tag="VHK28_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141638.1"
                     /translation="MTHRDPSDARPRRRRARGFLPLPAALGLLAAVFVGALLAPIPTI
                     PSASDARQVAERVEQRLPGWQVDRTLSSWEGAWTVVASCGNRQVGFQFVPGHGLRPGD
                     AWLHPQDEFTRTRLRFVSDDDVYLIWYRDEQRPRSLACNSELARRADEDRRSGRLD"
     gene            complement(4501..>4808)
                     /locus_tag="VHK28_05105"
     CDS             complement(4501..>4808)
                     /locus_tag="VHK28_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2141639.1"
                     /translation="VEVQAESEALKRPKRADSAEGGYAGYGVAEHKPHRPLKTNVEFY
                     AAAVLQGIGLAPSLFPATFALARHAGWTAHALEQGGANRLIRPDVRYIGPGERTLPV"
BASE COUNT          674 a         1694 c         1720 g          720 t
ORIGIN      
        1 gttcggatct gtaaaggggt tggcccgagg gcaccgacct cagctgcgct ccacctggcg
       61 ccgtccctgg agggagcgga tgagggtgac gtcgtccgcg tactcgaggt cgccgcccac
      121 cggcaggccg cgcgcgatgc gggtcaccac cggcacatgg ggctggagca ggtcggccag
      181 gtacatggcg gtggcctccc cctccaggtt tggattggtg gccaggatga cctcgccgat
      241 ggagccctcg gtcacccgag ccaccagctc ccggctgcgg atctgctcgg gaccgatgcc
      301 gtcgatgggg ctgatggcgc cgtgaagcac atggtagagc ccgcggaact cggccgtccg
      361 ctcgatggcc agcacgtcca ggggctcctc cacgacgcag atgcgggtgg gatcgcgacg
      421 agcgtcggcg cagatggcgc aggggtccac gccacggtcc gtgatgttgc agcacaccga
      481 gcagtaggcc acgtcctcct tgaccgagat cagggcggcg gccaggtcgc gcgcctcgtc
      541 ggccggggcg cgaaggacgt ggtacgccag gcgctgcgcg gtcttgcgac cgacgcccgg
      601 caggcggctg aattcgtcga tgagccgcgc gaccggcgcg atgagcccgc tcacggccga
      661 tgcgcctcat gcgccggcgc gcgatttcgc agggcgatca gcggccgggc agggcggggc
      721 cgcccatgcc tcccatcccc ggcatgccgg gcaaccccag gccgcccgcg acggccgaca
      781 tgcgctcccg ctcgaggtcc ttgctgcgcg tcaccgcgtc gttcaccgca gccatgacca
      841 ggtcctggag catctcgacc tcttcgggat ccaccgcgcc ggggtcgatt tcgatgtaac
      901 ggatctcctg cgcgccggtg atcaccacgc gcaccgctcc cccaccggag gtcgtctcga
      961 gcgtcgtctc cttcagctcc tcctgggcct gggccatctg cgcctgcatc tgctgggcga
     1021 tcttggtgat ctgaccgatg ttcacatgct tctcctggat tgacggtggc ctcagcggac
     1081 ctcggggacg tcgacgagct cgccgccggt gatgcgcagg acgccgtcca gcagggcctg
     1141 gccgtcggca tcggatgggt ctgcgacgac cgcctgggcc acgctcagcg gctcgagctc
     1201 gacgttgctg gccacgcagt cgatggccca tgcaccaccc agcacctgcg ccagcagctg
     1261 ctcgatagcg ggcgcgcgcg tgcgcgcctt ctctcgcatg aatgcccgct cctcggggaa
     1321 ggccagcgtc aggcgcgcgc cgtccacgct gaccggccgg cagtcgctga ggagcggctt
     1381 gatggcgggc gcagccttct gcacaacctc gccccatcgg gcgcgcagtt ccgccacctc
     1441 aggacgctcg ccgggggtgg cggccacggg ctcgggctcc ggtgcggcct gctcctcgac
     1501 cgcagccgcc tgacgcgccg cgggcgcgga cgttgcctgg acggcgggtt tgggcgccag
     1561 cggcggccca gatcgctcca ccttcgccac ctccggcgcc ggtttggggg ccacgggccg
     1621 cgccgcagcc acggccacgg ccggcgcctc agctgtcggg gcaagcccat ccacggccag
     1681 cagctcgagc gccaggcggg cgcgtccctc acgggcgccg gcaccggcgg cctcggcgac
     1741 ggtgcgcagg atgtgggcaa ggcgtcgtgc cagctgcggg tcggcggcgg cggcgagcag
     1801 ccggcggcgt gcctcaacct cggcctgggc ggccacctgg cccatgtccc gcccgcggtc
     1861 cacgagcccc tcgatgccgt ccaaggcagc gccggcatcg ccggcgacgt aggcgtccac
     1921 cagggcgacg atcgcctcgt catcggccag gccaaccgcc tcgcgaacgt cttcggcggt
     1981 cagccgctcg ccggcgaagg ccagcacctg gtccagcagc gactcggcgt cgcgcatgcc
     2041 gccgtccgcc aggcgggcga tcagggtcag cgcctccggc tcggcatcgg cctcctccgc
     2101 gtccacgatg cgccccagct tgccggcgat ggcatcggtc ggcagccgcc ggaagtcgaa
     2161 gcgctgggtg cggctgatca tggtggccgg caccttgtgg gtatcggtcg tggccagcac
     2221 gaacacgacg tggggaggag gctcctcgac catcttcagc agcgcgttga aggcgtgcgt
     2281 gctgagcatg tggacctcgt cgatgatgta gacccgccgc cgcagctccg acggcgcggt
     2341 cagcgcccgc atcaccaggt cgcgggcgtc ctccaccagg ccgtggctgg ccgcgtcgat
     2401 ctcgatgacg tccatggccc ggccctcgcg gatggagacg caggcggcgc acgtgtcgca
     2461 cggctcgccg tcctcgccgc ggttggtgca gttgatggcc ttggccagga tgcgcgcagt
     2521 cgaggtcttg ccggtgcccc gcgggccaac gaacagatag gcgtgggcca ggcggtcggt
     2581 ggcaaccgca cgccgcaggg tggtgacgat ggcgtcctgt cccagcacct ccgcgaagcg
     2641 ctgcgagcgc cagcggcggt agagcgcctt gtgctggtcg gcgggctgat ccgcgggctg
     2701 gtcgccgccc ggcgtggccg tcggggccgg cggcgaatcg gtgggcaggg acgtcgacat
     2761 ggcctccgta ccgtacacca aggccggagc ttgatagcgg ccaccggggg gcggccgccg
     2821 gccaaggcag aagtgaagag ggagcggtcg tgcacccggc gtcgatcgag agccaaccac
     2881 accacgggat cgcccggctc cagccaggtt gccccacggc acccgaaggg gaccgcttac
     2941 cgctgcttcc ttccggacct gacggggttc acagaccttc gctgcgcggg acccgactct
     3001 caacgtcgga cgagttccca tgggaggcgg aggctcgtcg gcctcggccg gggattcagc
     3061 cccgctgtag cggattgcgg gtacagggca ccgctagttc cccgcctagc acgaccgtga
     3121 tcgatggtaa ggccagcgtg ggtaacggtc aattcgacac gctcgaatgg agtggcggag
     3181 agggggggat tcgaaccccc gacgggtttc cccgaaccgc atttccagtg cggcgccata
     3241 ggcctctagg cgacctctcc gcgcgaacgg gcaaccgcga cggcgggcga atcggtgcgg
     3301 gtgggggtgt ggcggagagg gtgggattcg aacccacggt gctcatcgca caccgctttt
     3361 cgagagcggc accttaaacc actcggacac ctctccggac gaagagtcta tcagaggatg
     3421 gaccgatgcc tcccacgcgg aggcggctac ttggcgacgg cggacccgtt cgccaggcgg
     3481 cgcacctctg cctcgcgcac gccggagatg accgacaact ggtgcggcag gccggacgcc
     3541 cgggcaaggt cgatggctcc cccggcggcg cccttggcgc tgtccggcgc ggcgtagatc
     3601 agcgagccga cctgcgcctc cagcagcgcc ccgacgcaca tggcgcatgg ctcgtgggtg
     3661 gcgaagatgg agatctcgga caggcgccgc gactcgagct tgcgggcggt ggcctggagg
     3721 gtcagcagca cggcgtgcgc ggtcgggtcg cgctgcagct gcatgcggtc gtggtcgcgg
     3781 gcgatcatgg cgtcgtccag gaccgcgacg gcgccgaccg ggatctcccc ctcctcggag
     3841 ccgcggcgag cctcggcgat ggcctctgcc atgaagagct cgtagttcat cggtcctcgc
     3901 gctgtgggaa cccgtgggac gagcacggag tctaacagag gtgggcatag atcctgctct
     3961 atgctgcctg accgaaatcg gtagattcac cgcgtccaga tgttgagcca acgttgaccc
     4021 accgcgaccc atccgatgct cggccgcgcc gccgccgcgc gcgtggcttc ctgccgctgc
     4081 ccgctgcctt gggcctgctg gccgccgtct tcgtcggcgc gctgctggct cccattccca
     4141 ccatcccgtc ggcgtcggat gcgcgccagg tggcggagcg tgtcgaacag cggctgccgg
     4201 gctggcaggt cgaccggacg ctcagctcgt gggagggtgc ctggaccgtc gttgcatcat
     4261 gcggcaatcg gcaggtcggc ttccagttcg ttccgggcca tggattgcgc ccgggcgacg
     4321 cctggctgca tccgcaggac gagttcacgc ggacacggct gcggtttgtc tccgacgacg
     4381 acgtctacct gatctggtac cgcgacgagc agcggccgcg atcgctggcc tgcaacagcg
     4441 agctcgcccg gcgggccgac gaagatcggc gcagcggccg gctcgactga gggcccggcg
     4501 ctagacaggt agggttcgct ccccgggacc gatgtaacgc acgtccggcc ggatcaaccg
     4561 gttggcgccg ccctgctcca gcgcgtgcgc cgtccacccg gcatggcggg ccagggcaaa
     4621 ggtggccggg aagaggctcg gggccaggcc gatgccctgc agcacggccg ccgcgtagaa
     4681 ctccacgttc gtcttgagcg gccggtgcgg cttgtgctcg gcaacgccat aaccggcata
     4741 cccgccttcc gctgaatcag cacgcttcgg ccttttcaag gcctcgcttt cagcttgcac
     4801 ttccactc
//
LOCUS       DATACA010000244         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_594062, whole genome shotgun sequence.
ACCESSION   DATACA010000244 DATACA010000000
VERSION     DATACA010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594062"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VHK28_05110"
     CDS             <1..220
                     /locus_tag="VHK28_05110"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2141640.1"
                     /translation="VVDYLFATKKQHRIQLVIVPENGASRRIAEKCGFVLEGTARGAF
                     YNDGHNQDVVIYSLLRTDPRPWHQRADR"
     gene            217..588
                     /locus_tag="VHK28_05115"
     CDS             217..588
                     /locus_tag="VHK28_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141641.1"
                     /translation="MTALRRVAAVGGLLVLAACAAQAAGGLPTYPSDFCSPAELLSPV
                     RITIEPDRDPPVWGTRTESDERLELLWPADYSLREEGTEGLVVVDGEGRVIARDGTVL
                     NGVAGGTDGSGRVELCPIEAV"
     gene            complement(810..1328)
                     /locus_tag="VHK28_05120"
     CDS             complement(810..1328)
                     /locus_tag="VHK28_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141642.1"
                     /translation="MSQLLRLAIAAFLLAGCTTASSTATPEPTAPAPSTSPTPEATDS
                     PRPTDTPATPSPVASEPAGEGPLLATFRVSGQEEYRILLRETDDIELARAWEAGEFER
                     LIPNGRVVMGDDGGVNTGYDWHIDPQDVELAEITVEVCDGLPSYIEDGILSGDRFCPW
                     NAELVSLEPYEE"
     gene            1481..>1867
                     /locus_tag="VHK28_05125"
     CDS             1481..>1867
                     /locus_tag="VHK28_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141643.1"
                     /translation="MRRLASLLAAGVATLALAGPTLACGGLVGPNGAVNLLRTTTFAG
                     YHDGVEHYVTAFEFAGGGGAFGSIVPLPGVPTSVERGGDWTLQRLVLETAPERFSLFS
                     GDAALAGAESAQVLLETRIDALDITVL"
BASE COUNT          268 a          616 c          678 g          305 t
ORIGIN      
        1 ggtggtcgac tacctgttcg cgaccaagaa gcagcaccgc atccagctgg tgatcgttcc
       61 cgagaacggt gcgtcgcgac ggatcgccga gaagtgcggc tttgtcctgg agggcacggc
      121 gcgcggcgct ttctacaacg atgggcacaa ccaggacgtc gtcatctact cgctgctgcg
      181 caccgatcct cgcccctggc accagagggc tgaccgatga ccgcactccg ccgagtggcc
      241 gcggtcggcg gcctgctggt gctggcggca tgcgctgccc aggccgcggg aggactgccc
      301 acctacccgt cggatttctg ctcgccggcc gagctgctgt caccggtacg tatcacgatc
      361 gagcctgatc gcgatccgcc ggtctgggga accaggacgg agtccgacga gcgcctggag
      421 ctgttgtggc cggcggacta ttctctgcgc gaagagggga ccgaaggcct tgtggtcgtg
      481 gacggcgagg gccgggtgat cgcgagggac ggcaccgtgc tcaatggtgt cgctggtggc
      541 accgatggct ccggccgcgt ggagttgtgc ccgatcgagg cggtgtgacc ggctcctgag
      601 gagtcccgcc gacaggtggc cgtggatggg tgccgtttag tcgtgaggcg actgccagtc
      661 catgcccggg aggcactcga agtgccatgt catgcacggg atgcttacgg tggcgcccca
      721 acggccgcgg cggcggattg gtatgcatgc cgcgcatgaa tggcgcccga tggagcccgc
      781 ggcccggcgg cggccgcgac cgacagcgcc tactcctcgt aaggctccag cgatacgagc
      841 tcggcgttcc acgggcagaa gcggtcgccg ctgaggatgc cgtcctcgat gtaggagggc
      901 agcccatcgc agacctccac ggtgatctcg gccagttcca cgtcctgcgg atcgatgtgc
      961 cagtcgtagc cggtgttcac cccgccgtcg tcgcccatca cgacccggcc gttggggatc
     1021 agccgctcga actcgcccgc ctcccaggcg cgtgccagct cgatgtcatc ggtttccctc
     1081 agcaggatgc ggtactcctc ctgtccggac acccggaagg tggcgagcag cggaccctcg
     1141 ccggccggct cggaggcgac cggcgacgga gtggcggggg tatcggtcgg ccgaggtgaa
     1201 tcggtcgctt ccggcgtggg agacgtgctg ggagcgggcg cagtcggctc gggcgtcgcc
     1261 gtgctggagg cggtggtgca ccccgcgagc aggaacgcgg cgatcgcgag gcgcaggagc
     1321 tgggacatgg ttcctccgga ggctgggatt cgcgcggcga ggtgagcgcc gaactggtgt
     1381 gcatcggtct tgggggaaca ccatgctgac ccagaccgtc ctgccccaca agtgagccga
     1441 ccgtactggg ccggctcgga tcgcacggga ggacgaaacg atgcgacgac tagcttccct
     1501 cctggcggcc ggcgtcgcca ccctggccct cgctggtccc acgctggcgt gcggcggcct
     1561 ggtcggtccg aacggggccg tcaacctgct gcgcacgacc accttcgccg gctaccacga
     1621 cggcgtggag cactacgtga ccgcgttcga gtttgccggg ggtggcggcg cgttcggcag
     1681 catcgtgccg ctgcccggcg ttccgaccag cgtggagcgc ggtggcgact ggaccctcca
     1741 gcgcctggta ctggagaccg ctccagagcg gttctcgctc ttttccggtg atgcggcact
     1801 ggccggtgcc gagtccgccc aggtcctgct cgagacccgc atcgacgcgc tggacatcac
     1861 cgtgctg
//
LOCUS       DATACA010000245         3717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1398157, whole genome shotgun sequence.
ACCESSION   DATACA010000245 DATACA010000000
VERSION     DATACA010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398157"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1027)
                     /locus_tag="VHK28_05130"
     CDS             complement(71..1027)
                     /locus_tag="VHK28_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2141644.1"
                     /translation="MDTLLMAIIVVIGVPGAVVAYIIATEYVLRAVKPLQRGKIRPWL
                     WVAPAFIFLIAFLIYPTIRTLIVSFMNARGDQFVGLDNYIRLFTSGGFWQTLLNNAIW
                     IVFFTGLVVMLGIVIAVLTDRVRYETVVKAVIFIPMAISFVAAGVIWSFIYNFDADIG
                     TVNAVITAVGGEAEPLLLEWPRNTFLLVIVGIWMWTGFAMVILSAGLKGISTELLEAA
                     RMDGANEFQVFFRIILPLLAPTIAVVATTIVITALKTFDIVYVMTGGRFGTEVIGTLF
                     YRELFTNNNDGAAAAVAIILLLAIVPVMLFNIRRFQAQEAIR"
     gene            complement(1107..2408)
                     /locus_tag="VHK28_05135"
     CDS             complement(1107..2408)
                     /locus_tag="VHK28_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2141645.1"
                     /translation="MRYLAPTAIVVALAMVLAACQQGEDGSPGASGGAQGGTVNLLVV
                     FGGEELANFEAMLAPFEEETGITVEIESNRDANAILQTRIDGGNPPDVAGLPGPGPMA
                     EFARAGHLKPMPEEVANILNEQYDEGWVVAGTVDDQLVGVFVKTSLKGLIWYNPEQFE
                     AAGYEVPEDWDGLVSLADQIAADGTPPWGIGLESGAASGWPATDWIEDFVLRQSGPDV
                     YDAWVDGEQAWTSPEIREAFEAFGMWATDPEYVAGGPNTVLSTAFGNGGDCMFTDPPE
                     CYLHHQASFMSGFFEENFPEVAVAGETYDYFMMPPIEFDAITTAGDLFGMFNDTDAAQ
                     QLMAYLVSAEAQQAWVETGGVLSANQDVSLDAYPDDATRQAAEIMTSAETTRFDGSDR
                     MPASMNDAFFGAILEYVQNPDNLDSILEELDAAQAEAYAEG"
     gene            complement(2562..3650)
                     /gene="ugpC"
                     /locus_tag="VHK28_05140"
     CDS             complement(2562..3650)
                     /gene="ugpC"
                     /locus_tag="VHK28_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX2141646.1"
                     /translation="MATVTFDHVYKRYSADVIAVNDLNLEIADGEFLVLVGPSGCGKT
                     TALRCVAGLETISDGKLYIGDRVVNDVAPKDRDIAMVFQSYALYPHMSVYDNLAFGLK
                     LRKTPKADIDRRVKEAAGILGLERFLDRKPKALSGGQRQRVVLGRAIVREPAVFLMDE
                     PLSNLDAKLRVQTRAEIARLHQRLGTTTVYVTHDQVEAMTMGDRIAVMRDGLLQQVGT
                     PQELYDRPRNVFVAGFIGSPAMNFATTRTEGEQLMLGNARLDLSGAAAQAAKSKPAGS
                     ELLIGFRPEHLDVADGRGDTVRIPARVDVVEYLGNEELIHAQAEGNEVVALIPSDRRV
                     KPGDQVDLTVPVDKLHVFDPETEKSLVA"
BASE COUNT          590 a         1208 c         1285 g          634 t
ORIGIN      
        1 aggctggcga gggcggccgc gacaccggtg cgctcgcggg cggcctgggg ctgagcgccg
       61 ttgatcgccg tcatcgaatc gcctcctgcg cctggaaccg gcggatgttg aagagcatga
      121 cgggcacgat ggcgagcagc aggatgatcg cgaccgcggc cgcggcgccg tcattgttgt
      181 tggtgaacag ctcgcggtag aacagggtgc cgatgacctc ggtcccgaat cggccgccgg
      241 tcatgacgta cacgatgtcg aacgtcttga gggcggtgat cacgatggtg gtggccacca
      301 ccgcgatggt cggtgccagc agcggcagga tgatgcggaa gaagacctgg aactcgttgg
      361 cgccgtccat acgggccgcc tcgagcagct cggtgctgat gcccttcagg ccggcggaca
      421 ggatgaccat ggcgaagccg gtccacatcc agatgcccac gatgaccagc aggaaggtgt
      481 tgcgcggcca ctccaggagc agcggctcgg cctcgccacc cacggctgtg atgaccgcgt
      541 tgacggtgcc gatgtcggcg tcgaagttgt agatgaagct ccagatgacg ccggcggcca
      601 cgaagctgat ggccatggga atgaagatga ccgccttcac cacggtctcg tagcgcaccc
      661 ggtcggtgag caccgcgatg acgatgccca gcatcaccac cagcccggtg aagaagacga
      721 tccagatggc gttgttcagc agggtctgcc agaacccgcc actggtgaag aggcgtatgt
      781 agttgtccag ccccacgaac tggtcgccgc gggcgttcat gaacgagacg atcagcgtcc
      841 ggatggtggg gtagatgagg aaggcgatca ggaagatgaa ggcgggtgcc acccacagcc
      901 agggacgaat cttcccgcgc tgcaggggct tgaccgcgcg cagcacgtac tcggtggcga
      961 tgatgtacgc aaccacggcc cccggaacgc cgatgacgac gatgatcgcc atcagaaggg
     1021 tgtccacctg ggctgacctc cttcccgctg cgctttatgg accgatggag ccgggcggcc
     1081 cgtgcgggcc acccggctct caggtgttac ccctcggcgt aggcctcggc ctgcgccgcg
     1141 tcgagttcct ccaggatcga gtccaggttg tccgggttct ggacgtactc caggatggcg
     1201 ccgaagaacg cgtcgttcat cgatgccggc atccggtcgg acccgtcgaa gcgggtcgtc
     1261 tcggcgctgg tcatgatctc ggcggcctgc cgtgtggcgt cgtccggata ggcgtcgagc
     1321 gaaacgtcct ggtttgccga caggacgccg ccggtctcca cccaggcctg ctgggcctcg
     1381 gcgctgacca ggtaggccat cagctgctgg gctgcatcgg tgtcgttgaa catgccgaac
     1441 aggtcaccag cagtggtgat ggcatcgaac tcgatgggcg gcatcatgaa gtagtcgtag
     1501 gtctcgcccg cgactgcgac ttcggggaag ttctcctcga agaagccgct catgaagctc
     1561 gcctggtggt gcaggtagca ctccggcggg tcggtgaaca tgcagtcgcc gccgttgccg
     1621 aaggccgtcg acagcacggt gttcgggccg ccggcgacgt actcggggtc ggttgcccac
     1681 atgccgaagg cctcgaaggc ctcgcggatc tcggggctgg tccatgcctg ctcgccatcg
     1741 acccacgcgt cgtagacgtc cgggccggac tggcgcagga cgaagtcctc gatccagtcg
     1801 gtggccggcc agccgctggc cgcgccgctc tccaggccga tgccccacgg cggagtgccg
     1861 tcagccgcga tctggtcggc cagcgagacg agcccatccc agtcctcggg gacctcgtag
     1921 ccggccgcct cgaactgctc cgggttgtac cagatcagcc ccttgaggct ggtcttgacg
     1981 aagacgccga ccagctggtc gtcgaccgtg ccggccacga cccagccctc gtcgtactgc
     2041 tcgttgagga tgttcgccac ctcttcgggc atgggcttga ggtggccggc gcgcgcgaac
     2101 tcggccattg ggccggggcc cggcaggccg gcgacgtccg gcgggttccc gccgtcgata
     2161 cgggtctgca ggatcgcgtt cgcgtcgcgg ttgctctcga tctcaaccgt gatgcccgtt
     2221 tcctcctcga agggcgcgag catcgcctcg aagttcgcca gctcctcgcc gccgaacacc
     2281 acgagcaagt tgacggtgcc tccctgggcc ccgccgctcg cgccagggct gccgtcctcg
     2341 ccctgctggc aggcggccag caccatggcg agggcgacga cgatggcagt cggcgccagg
     2401 tatctcattc tccgggtcat gcaactcctc cagcgcgagt tggcggcggc gtgtccgcct
     2461 ccaatctggg tgatcggccc gcgaccgcat ggcggttcgc gagcctgaaa caggggatcg
     2521 gggtcaatac ctccgtctac tcatcgccag ccgcgcgttg gtcacgcgac cagcgacttc
     2581 tcggtctccg ggtcgaacac gtgcagcttg tcgactggca cggtcaggtc cacctggtca
     2641 ccgggcttca cgcggcggtc cgacgggatg agggcgacga cctcgttccc ctccgcctgt
     2701 gcgtggatca actcttcgtt gcccaggtac tccacgacgt cgacgcgggc ggggatgcgg
     2761 acggtgtccc cacgcccatc cgccacgtcg aggtgctccg ggcgaaagcc gatcagcagc
     2821 tcgctgccgg cgggcttgct cttggcggcc tgcgcggcgg cgccggacag gtcgagcctg
     2881 gcgttgccga gcatgagctg ttcaccctcg gtgcgggtgg tggcgaagtt catggcgggt
     2941 gaaccgatga agccggcgac gaacacgttg cgtgggcggt cgtacagctc ctgcggcgtc
     3001 ccgacctgct gaaggaggcc gtcgcgcatg accgcgatgc ggtcccccat ggtcatggcc
     3061 tcgacctggt cgtgggtgac gtagacggtg gtggtgccca gccgctggtg gaggcgcgcg
     3121 atctccgccc gcgtctggac ccgcagcttg gcgtccaggt tggagagcgg ctcgtccatc
     3181 aggaagacgg ccggctcgcg cacgatggcc ctccccagca ccacgcgctg ccgctggccg
     3241 ccggacaggg ccttgggctt gcggtccagg aagcgctcca ggcccaggat cccggctgcc
     3301 tccttgacgc gccgatcgat atcggccttg ggcgtcttgc gcagcttcag tccgaaggcc
     3361 agattgtcgt agaccgacat gtgggggtag agggcatagc tctggaagac catggcgatg
     3421 tcccggtcct tgggggcgac gtcgttgacg acgcggtccc cgatgtagag cttgccgtcg
     3481 ctgatggtct cgaggccggc cacgcatcgg agggcggtcg tctttccgca tcccgatgga
     3541 ccgaccagca ccaggaactc cccgtcggca atctccaggt tgagatcgtt cacggcgatc
     3601 acgtcggcgc tgtagcgctt gtagacgtgg tcgaaagtca cggtcgccaa tgcaggtcct
     3661 ccaagaagag aggtgcgccc gtctcggatg ggcgacacac cgcgaggctg ccatcat
//
LOCUS       DATACA010000246         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_353557, whole genome shotgun sequence.
ACCESSION   DATACA010000246 DATACA010000000
VERSION     DATACA010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353557"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1166)
                     /locus_tag="VHK28_05145"
     CDS             complement(327..1166)
                     /locus_tag="VHK28_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141647.1"
                     /translation="MTAATLAPTATAPQPGSLRANLRLFWLGGLISYRALFNWARPSI
                     YIPTLIGAPTFQILFFAALGSYATGMGTEYFAVGNAVQVSAMSGIYGMTMAIANERWF
                     GTLHALLATPASRWAIFGGRFIPFIANGLLVSVYGFTISWLFLGVEIAPGSLGVIALA
                     LLVTVFSCSAIGAVQGGLSMRLRDGLFGANLLVFAFLLFCGVNIPLEALPGWMQAVSQ
                     VLPFTHGLQAVREAVDGAGLGQVGGLIAIEFLIGAAYALLAFGLFSYLERSARANATL
                     DVR"
     gene            complement(1178..1972)
                     /locus_tag="VHK28_05150"
     CDS             complement(1178..1972)
                     /locus_tag="VHK28_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141648.1"
                     /translation="MEGLQRNARLTWRVWIFTLRSFLASEFFILTAVLQPVIFASIAF
                     FLFRAGGAQAGEQTLLFAALGTGMMGVWSTTLFGCGGAIQWHRWEGTLELLVAAPSRY
                     DMVLLGQTLGAATFGFYSIAATLAWGVVLFGMPLNAEFPLLLPFALAVAIVSLGCLGM
                     LLATSFVLYRHANALSNLLEYPIWIVTGMIVPLAVLPGWLGPISWILVPTWAMEAIRG
                     SAMGGSPPWLALLMCVVLAVIYYLLARWVLRFVLDKARRDATLALA"
     gene            complement(1977..2999)
                     /locus_tag="VHK28_05155"
     CDS             complement(1977..2999)
                     /locus_tag="VHK28_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141649.1"
                     /translation="MPDVPAIEALDLVRVYRTHTGVIRRRKLDVPAVNGVSFDVRPGE
                     LFGLLGPNGAGKTTTIKMLITLLLPTSGTARVMGHDVVTQTNEVRKHIGYVFGGDRGL
                     YDRVSAMDNLRYFAELYGVDPSRQKQRIAALLELVGLTGREQEKVEGYSRGMRQRLHV
                     ARGLLHDPQVVFLDEPSIGLDPVGARELRQTIKTLVEAGKTVLLTTHYMFEADALCDR
                     IAVINDGRIVAMGTGADLKGAIANRTIVEIETFGVSPDAVDALRVDPEVIAVNMEERD
                     QSQILLVQSERGTQITQRLLQQLGDTRVGKVVGREPTLEDAYVHLIATTSGTDGSAAR
                     AAAVSA"
     gene            3177..>4546
                     /locus_tag="VHK28_05160"
     CDS             3177..>4546
                     /locus_tag="VHK28_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141650.1"
                     /translation="MAITRRIGLGLAARGEVGEVAAWAAAARRTGLDSVWIHDSYFER
                     DAVTYVSAIAAEIGADESEDGFRIALGAVNPFTRHPVVLAMTGSALDEMAPGRIVMGL
                     GTGLPLRLKQMGIPYAPDAAVEQVGAAMDQLRALWAGERLPSATEGLPPIQPMFPPPH
                     RIPLVIAAYRKEFVQLAGQKADGYLARPAESIPSLTGILRRLREAAQAAGRDAGSVES
                     AGYLLTLVDSSRREALNRAKREPFVIYMMSVLGDVSLARAGFERELRDRIAAAWRAEE
                     FHAAAELIPDDLLDAFMLCGTVEEVAAGALAFHERAGLELPLLQPVLQEEAQVSELLR
                     AAEAYGRLPEAAAERWGGIVGAEGDVPPASLPETGDERPFSGGWLRRRAGAVAEVLRP
                     FSFTASVVPIAAGGALAFVHGAFDAGLFFLALLAGVLLHIGTNVVNEVYDVRQGIDTI
                     TSPRAS"
BASE COUNT          732 a         1525 c         1622 g          667 t
ORIGIN      
        1 accgaatgcg taccgatgag gtgcgcccgc gcacatgacc gccgtgcgtc aggtgacggt
       61 acttgcaggc ggtgcgaccg ccttctcctc ccgggcggct ctcatatgcg actgctgctc
      121 gcgttagaga gctgttcaca gtcgatgacg gccttgtcgg ggccccgaag acgccaattc
      181 gtgctcgggt ccgccgctgt ggaaggcgga atcggggttc aatcgtgccg attcctctct
      241 aacgcgattg gtgttcgcgc ctgacagtgc caatcgaccg gggagcgaaa aacgcgaatg
      301 ggagtcgcgc cggagagcag cagccgtcag cggacgtcaa gggtggcgtt ggcgcgtgcc
      361 gagcgctcca ggtacgagaa cagcccgaac gccagcagtg cgtaggcggc cccgatcagg
      421 aactcgatcg cgatcaatcc gcccacctgg cccagcccgg cgccgtcgac ggcctcgcga
      481 accgcctgca ggccgtgggt gaacggcagc acctgggaca cggcctgcat ccagccggga
      541 agcgcctcga gcgggatgtt cacgccgcag aacagcagaa aggcgaagac caggaggttc
      601 gcgccgaaca gcccgtcccg cagtcgcatg gacaggccgc cctgcacggc accgatggcg
      661 ctgcaggaga agacggtgac cagcagcgcc agggcgatca cgcccaggct gccgggcgcg
      721 atctcgactc ccaggaacag ccagctgatg gtgaacccgt agaccgatac cagcaggccg
      781 ttggcgatga acgggatgaa gcgaccaccg aagatcgccc agcggctggc cggcgtggcc
      841 agcagggcgt gcagcgtgcc gaaccagcgc tcgttggcga tggccatggt catgccgtag
      901 atgccgctca tggcgctgac ctgcaccgcg ttgccgacag cgaagtactc ggtgcccatg
      961 cctgtcgcgt aggagcccag cgcggcgaag aacaggatct ggaaggtcgg cgcgccaatg
     1021 agggtcggga tgtagatgga gggacgtgcc cagttgaaca gcgcccgata gctgatcaga
     1081 ccgcccagcc agaacaggcg caggttggcg cgcaggctcc ccggctgcgg ggcggtggct
     1141 gtcggcgcga gcgtggcggc agtcaacgca gagcccccta cgccagcgcc agggtggcgt
     1201 cgcggcgcgc cttgtccagg acgaagcgca ggacccagcg cgccagcagg tagtagatca
     1261 cggccagcac cacgcacatc agcagcgcca gccacggcgg gctgccgccc atggccgacc
     1321 cgcggatggc ctccatggcc caggtcggga ccaggatcca ggagatcggt cccagccagc
     1381 ccggcaggac ggccagcggc acgatcatgc cggtcacgat ccagatgggg tactcgagca
     1441 ggttcgacag ggcgttcgcg tggcggtaga ggacgaacga ggtcgccagc agcatgccca
     1501 ggcagcccag cgacacgatg gccaccgcca gcgcgaaggg cagcagcagc gggaactcgg
     1561 cgttcagcgg catgccaaag agcaccacgc cccaggccag ggtggcggcg atcgagtaga
     1621 agccgaaggt ggcggccccc agcgtctgtc ccagcaggac catgtcgtag cgcgacgggg
     1681 cggcaaccag cagctccagg gtgccctccc agcggtgcca ctggatggct cccccgcagc
     1741 cgaagagcgt cgtgctccag acgcccatca tcccggtgcc cagggcagcg aacagcaacg
     1801 tctgctcgcc ggcctgcgcg cccccggcgc ggaacaggaa gaacgcgatc gacgcgaaga
     1861 tgaccggctg caggaccgcg gtcaggatga agaactcgct ggccaggaac gagcgcagcg
     1921 tgaagatcca gacccgccag gtcaggcgcg cgttgcgctg cagcccctcc atcggtctag
     1981 gccgatacgg ccgccgcgcg ggcggccgat ccatcggttc cggaggtggt ggcgatcagg
     2041 tgcacgtagg catcctccag ggtcggctcg cggccgacta ccttgccgac gcgcgtgtcg
     2101 cccagctgct gcagcaggcg ctgggtgatc tgggtgccgc gttcggactg gaccagcagg
     2161 atctggctct ggtcgcgctc ctccatgttc accgcgatga cctcgggatc cacccgcagg
     2221 gcgtcaacgg cgtcaggtga gacgccgaag gtctcgatct caacgatggt gcggttggcg
     2281 atggcgccct tgaggtccgc gccggtgccc atggccacga tgcggccgtc gttgatgacc
     2341 gcgatgcggt cgcagagcgc atccgcctcg aacatgtagt gggtggtgag caggaccgtc
     2401 ttgcccgctt ccaccagggt cttgatggtc tggcgcagct cacgagcgcc caccggatcc
     2461 aggccgatgg atggctcgtc aaggaagacg acctgcgggt cgtgcagcag gccgcgcgcc
     2521 acgtgcagcc gctggcgcat gccgcgcgag tagccctcga ccttctcctg ctcgcgcccg
     2581 gtgaggccca ccagctccag cagggccgcg atccgctgct tctgccggga ggggtctacg
     2641 ccgtacagct cggcgaagta gcgcaggttg tccatggcgc tgacccggtc gtacagtccg
     2701 cgatccccac cgaacacgta gccgatgtgc ttccgcactt cgttggtctg ggtcaccacg
     2761 tcgtggccca tcacccgcgc ggtgccggag gtgggcagca gcagggtgat cagcatcttg
     2821 atggtggtcg tcttgccggc gccgttgggt cccagcaggc cgaacagctc cccgggccgc
     2881 acatcgaagg agacgccatt caccgccggc acgtccagct tccggcgccg gatgacgccg
     2941 gtgtgggtcc gatagacgcg gaccaggtcc agcgcctcga tggcgggtac gtcgggcaca
     3001 gcggtggctg gaaggcttcg actcatgggc gccgcatttt ggacggatga ctagttcgat
     3061 gtcaagaggt ttgaagtaga gagggcgccg cgcaaggtcg gcaggttttc cagcctgcgc
     3121 tggcgggtgt aatctgctct catcaggtag ccgatttccc gggcaggagg tggatagtgg
     3181 ccatcacgcg gcgtatcggc ctggggctgg cggcccgcgg cgaggtcggg gaggtcgcgg
     3241 cctgggcagc cgcggcgcgg cggaccgggc tcgactcggt gtggattcac gactcgtact
     3301 tcgagcgcga cgcggtcacc tacgtcagcg ccattgccgc cgagatcggt gccgacgaga
     3361 gcgaggacgg tttccgcatc gccctcggcg cggtcaaccc cttcacccgc catccggttg
     3421 tgctggccat gaccggctcg gccctggacg agatggctcc gggccgcatc gtgatgggcc
     3481 tcggcaccgg gttgccgctg cgcctgaagc agatgggaat cccgtacgcg ccggacgccg
     3541 ccgtcgaaca ggtcggcgcc gccatggacc agctgcgggc gctgtgggcg ggggagcgcc
     3601 tcccatctgc gaccgagggc ctgccgccga tccagcccat gttcccgccg ccgcatcgca
     3661 tcccgctggt catcgcggcc taccgcaaag agttcgtgca gctggctggc cagaaggcgg
     3721 acggctacct ggcacggccg gccgagtcca tcccgtcgct gaccggcatc ctccgtcgcc
     3781 tgcgcgaggc ggcccaggcc gccggccgcg acgccggctc ggtggagtcc gcgggctacc
     3841 ttctgaccct cgtcgactcc agtcgccggg aggcgttgaa ccgtgcgaag cgcgagccgt
     3901 tcgtcatcta catgatgagc gtgctgggtg acgtctcgct ggcccgcgcc ggcttcgagc
     3961 gcgagctgcg cgaccggatt gccgccgcct ggcgcgccga ggagttccac gcggccgccg
     4021 agctgattcc ggacgacctg ctcgacgcgt tcatgctgtg cggcaccgtc gaggaggtgg
     4081 ccgccggagc gctggccttc cacgagcgtg ccgggctcga gctgccgctg ctgcagccgg
     4141 tcctgcagga ggaggcgcag gtcagcgagc tgcttcgggc cgcggaggcc tacggccggc
     4201 ttccagaggc cgcggcggag cggtggggcg gaatcgtggg cgccgaaggt gacgtcccac
     4261 ctgcctcgtt gcccgagacg ggggacgaaa ggccgttcag cggtggttgg ctgcgccgcc
     4321 gtgcgggcgc ggtggccgag gtgctgcgtc ccttctcgtt caccgcctcg gtggtcccca
     4381 tcgcggccgg cggcgcgctg gccttcgtgc acggggcgtt cgacgccggg ctgttcttcc
     4441 ttgcgctgct ggcaggcgtg ctcctgcaca tcggtaccaa cgtggtgaac gaggtctacg
     4501 acgtgcggca gggcatcgac accatcacct ccccgcgcgc cagcca
//
LOCUS       DATACA010000247         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2522703, whole genome shotgun sequence.
ACCESSION   DATACA010000247 DATACA010000000
VERSION     DATACA010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522703"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..3617
                     /locus_tag="VHK28_05165"
     CDS             27..3617
                     /locus_tag="VHK28_05165"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2141651.1"
                     /translation="MPGDHLVLLARDAAGYSALSRLASRGHLAGEKQFPIFERTLVEE
                     ALDSARGHLVGLSGCRNGEVARRLLAGEETTARAAAERWASHFPDGDFAVELSHHLLP
                     DDDWLTAQLAELAHAAGLPTVVTNEVHYADPGGHRLQDVLVCIRHGATLDDARELLLP
                     NAEYRLKTGTELAAIGRDLPDAKARRAWEAGLARAADIGQACRLDLNFERYRFPGFTV
                     PEGETPFSYLYQLAHEGLRHRYRPITKQAVKQLAHELDIIERTGLAEFFLIVWDLMEF
                     ARKGGIIGQGRGSAGDSIVAYCLGITKVDPIEHKLLFERFINEARTLPDIDIDFDVNR
                     REEVIQYLYTRYGEDHAAMVCTVITYRARSAVREVAKALGFPLDAVDRVAKALDTRDA
                     SDVARDLALDCSFAWLFDEIGVPMTDAAVPPGRDGRASDSPWHAGNRPAGPLPLRPDL
                     ADAEARARDAAADHDGRPYSESGWVPPRRPSVLDQSVVSVKHEGWQQKRYAGGAADLA
                     LPEPRDNGTPGPSSERPAVVRVDPESGMPVEERRRPSVVPGGDAASRSHQIAASQRLG
                     ADGAGFEGQRHRNTQLAGAGNAPNDSQAAIWQQGVAQATPIHAQPRNRWQWLLALCAE
                     IDGFPRHLGIHVGGMLVTRTPLIDLVPVERATMPGRVVTQYDKEDIEALGLVKIDLLS
                     LRTLGVVSECLDRIERDTGSRPDLDNLSHDDPAVFRTIQAADTVGMFQIESRAQMQAL
                     PKARPERFEDLVVQVAIIRPGPVQGNAVHPYLRRRAGQEPVVYLHDSLRPILEDTLGV
                     ILYQEQVMQVAIEVCGYTALEADIFRKAMGSHRSHAKMQAERGKFVGGAMRTGLTEED
                     ADELFRRCSAFAEFGFARAHAAAFAKITYDTAWLRHYYPTHYYAGVLNNQPMGFYSPA
                     VVLNDAKRHAVRVLPVDVNESSWEHDTRRLPDGKWALRLGLRQVKGIDEKARETFERE
                     RAIGPHAGVRDFVARTGLGEQVVERLIAIGAFDWTDMPRRELLWQLRTTMTDANPARP
                     ALGLTDDAQRALGASLPDMSLSERVAADYRELGLSQGAHAVELFRERLASRGVIQIAE
                     AAKRRDRSNIRLAGLAVSVQHPMTAKNFVFIALEDETGMINVTLRPEVYQAHRALLHR
                     HPLMVVDGRLQVEGAVLNVVARRLHAVDAVIAEDERETRLPLSKQQRMFR"
     gene            3643..4107
                     /locus_tag="VHK28_05170"
     CDS             3643..4107
                     /locus_tag="VHK28_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2141652.1"
                     /translation="MNRPDPSLDQQGSVRSFLRVGGLIALGIGILLTIGGMASFFSAF
                     GTFEQPRSFWMAFIGLPLTAVGGAMVRAGFLGPAARYVTGEVTPVLQDTLGALGIRSS
                     AQECVSCGARNDADSRFCDSCGKPLSRACPSCAAQNDADARFCDGCGKPLTA"
     gene            4212..>4636
                     /locus_tag="VHK28_05175"
     CDS             4212..>4636
                     /locus_tag="VHK28_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141653.1"
                     /translation="MLNGERISLRPVRATDVDAMWEAHTNIANRGAFFPLGVTSESAF
                     RREFGENGFWQREEGWLLIITPDGEMAGHIEFFKPVNYWDCFELSYQLYDDRFSGRGY
                     VSEAVQLVVDYLFATKKQHRIQLVIVPENGASRRIAEKCG"
BASE COUNT          713 a         1648 c         1603 g          672 t
ORIGIN      
        1 ggccggggga gcatcacgcc ggaccgatgc ccggcgacca cctggtcctg ctggcccgcg
       61 acgccgccgg ctactcggcg ctctcgcgcc tggcgtcgcg cggccacctg gccggggaga
      121 agcagtttcc gatcttcgag cgcaccctcg tggaggaggc gctcgactcc gcacgtggcc
      181 acctggtcgg cctgtccggc tgccgcaacg gcgaggtggc ccgccgcctg ctggccggcg
      241 aggaaacgac cgcgcgcgcc gccgccgagc gctgggcgag ccacttcccg gacggcgact
      301 tcgcggtgga gctcagccac cacctgctgc cggacgacga ctggctcacg gcgcagctgg
      361 ccgagctggc gcacgccgcc gggctgccca ccgtggtcac caacgaggtg cactacgccg
      421 atccgggcgg gcaccgcctc caggacgtgc tggtctgcat ccgccacggc gccaccctgg
      481 acgacgcgcg cgagctgctg ctgcccaatg ccgagtaccg cctgaagacg ggcaccgagc
      541 tggccgccat cggtcgtgac ctgccggacg ccaaggcgcg acgggcgtgg gaggccggtt
      601 tggcgcgcgc cgccgacatc ggtcaggcat gcaggctgga cctgaacttc gagcgctatc
      661 ggttcccggg cttcacggtc cccgagggcg agacgccgtt ctcatacctc taccagcttg
      721 cacacgaggg cttacgccac cgttatcggc cgattaccaa gcaggccgtc aagcagctgg
      781 cgcacgagct ggacatcatc gagcgcaccg ggctggccga gttcttcctg atcgtgtggg
      841 acctgatgga gttcgcgcgc aagggcggca tcatcggcca gggaaggggg agcgccggcg
      901 actcgatcgt ggcctactgc ctgggcatca ccaaggtcga ccccatcgag cacaagctgc
      961 tgttcgagcg cttcatcaac gaggcgcgca cgctgccgga catcgacatc gacttcgacg
     1021 tcaaccggcg agaagaggtg atccagtacc tctacacgcg ctacggcgaa gatcacgcgg
     1081 ccatggtctg caccgtgatc acttatcgcg cccggtccgc ggtgcgcgag gtggccaagg
     1141 cgctgggctt tccactggac gcggtggatc gggtggccaa ggcgctcgac acccgcgacg
     1201 cctcggacgt ggcccgcgac ctggccctgg actgctcgtt cgcctggctc ttcgacgaga
     1261 tcggcgtgcc catgaccgat gcggccgtcc cgcccggccg cgacggccga gcgtccgatt
     1321 ctccctggca cgccggcaac cgtccggcgg gcccgctgcc gctgcggccg gacctggccg
     1381 atgcggaggc ccgcgcccgg gatgccgccg ccgaccacga cgggcggccg tacagcgagt
     1441 cgggctgggt gccgccgcgg cggccgagcg tgctggacca gtcggtggta tcggtcaagc
     1501 acgagggctg gcagcagaag cgctacgccg gcggcgcggc cgacctggcg ttgccggagc
     1561 cgcgcgacaa cggcacgccg gggccgtcgt cggagcggcc cgcggtggtc cgcgttgacc
     1621 cggagagcgg gatgccggtg gaggagcgcc gacgcccgag cgtggtcccg ggcggcgacg
     1681 cggcgagtcg gagtcaccag atcgcagcgt cgcagcgctt aggcgcggac ggagctggat
     1741 ttgaaggtca aaggcatagg aatactcaac tagccggcgc gggcaacgcg cctaacgaca
     1801 gccaggctgc gatctggcaa caaggcgtcg cccaggccac gcccatccac gcgcaacccc
     1861 gcaacagatg gcagtggctc ctcgccctgt gcgccgagat cgacggcttc ccgcgccacc
     1921 tggggatcca cgtgggcggc atgctggtga cccgcacgcc gctgattgac ctggtgcccg
     1981 tcgagcgggc caccatgccg ggtcgggtgg tgacccagta cgacaaggag gacatcgagg
     2041 ccctgggcct ggtcaagatc gacctgctca gtctgcgcac gcttggcgtg gtcagcgagt
     2101 gcctggaccg catcgagcgc gataccggca gccgcccgga cctggacaac ctctcccacg
     2161 acgaccccgc cgtcttccgc accatccagg ccgccgacac ggtgggcatg ttccagatcg
     2221 agtcgcgcgc ccagatgcag gcgctgccca aggcccgccc cgagcgcttc gaggacctgg
     2281 tggtccaagt ggccatcatc cgccccggcc cggtgcaggg caacgccgtg cacccctacc
     2341 tgcgccgccg tgccggccag gaaccggtcg tctacctcca cgactccctc aggccgatcc
     2401 tggaggacac cctgggcgtg atcctctacc aggagcaggt catgcaggtg gccatcgagg
     2461 tgtgcggcta caccgcgctg gaggcagaca tcttccgcaa ggcgatgggc agccaccgca
     2521 gccacgccaa gatgcaggcc gagcgcggca agttcgtggg cggcgccatg cgcaccggcc
     2581 tgaccgagga ggatgccgac gagctcttcc gccgctgcag cgccttcgcc gagttcggct
     2641 tcgcccgtgc ccacgccgcg gccttcgcca agatcaccta cgacaccgcc tggctgcgcc
     2701 actactaccc gacccactac tacgccggcg tgctgaacaa ccagccgatg ggcttctact
     2761 cgccggcggt ggtgctcaac gacgccaagc gccacgcggt tcgcgtcctg cccgtggacg
     2821 tcaacgagtc gtcctgggag cacgacaccc gccggctgcc cgacggcaag tgggcgctcc
     2881 gcctgggcct gcgccaggtg aaggggatcg acgagaaggc gcgcgagacg ttcgagcgcg
     2941 agcgcgccat cggtccacac gccggcgtgc gcgacttcgt ggcccgcacc ggcctcggcg
     3001 agcaggtggt ggagcgcctg atcgccatcg gtgccttcga ctggaccgat atgccccggc
     3061 gcgagctgct ctggcagctg cgcacgacca tgaccgacgc caaccctgcg cgtcccgcat
     3121 tgggcctgac cgatgacgcc cagcgtgcgc tgggcgcctc gctaccggat atgagcctgt
     3181 cggagcgggt ggccgccgac taccgggagc tggggctgtc ccagggtgcc cacgccgtcg
     3241 agctcttccg cgagcggctc gcgtcgcgcg gcgtgatcca gatcgcggag gctgccaagc
     3301 ggcgcgatcg gtcgaacatc cggctggccg gcctggcggt atcggtccag cacccgatga
     3361 cggccaagaa cttcgtgttc atcgcgctgg aggatgagac ggggatgatc aacgtcaccc
     3421 tgcgcccgga ggtgtaccag gcgcaccgtg cgctgctgca ccgccacccg ctgatggtgg
     3481 tggacggccg cctccaggtg gagggggcgg tgctcaacgt ggtggcgcgc aggctccacg
     3541 cggtggatgc cgtcatcgcc gaggacgaac gggagacccg gttgccgctc tccaagcagc
     3601 agcgcatgtt ccgttgacgg cgcgtcctat actcctgccc gcatgaacag gcccgacccg
     3661 agcctcgacc aacagggcag cgtccggtcc ttcctccgcg tcggagggct gatcgccctc
     3721 ggtatcggca tcctgctgac catcggcgga atggccagct tcttcagcgc attcggcacg
     3781 ttcgagcagc cccgcagctt ctggatggcc ttcatcggac ttccgctgac ggcggtcggc
     3841 ggcgcgatgg tcagggccgg cttcctggga cccgccgcgc gctacgtgac cggcgaggtc
     3901 accccggtcc tgcaggacac gctcggcgcg ctggggatcc gctcatcggc ccaggagtgc
     3961 gtgtcgtgcg gtgcccgcaa cgacgccgat tcgcgcttct gcgactcgtg cggcaagccg
     4021 ctcagtcgcg cgtgcccctc gtgcgctgcg cagaacgacg cggacgcccg cttctgcgac
     4081 ggttgcggga agccgctgac cgcctgagga tcgccacccg cagcccggcc cctacgtgca
     4141 cgcacccagt ccagcgccgt tgacggcggc tggttgcagc cgacacgctt ccgcctcatg
     4201 gaggtgaacc gatgctgaac ggcgagcgga tcagcctgcg cccggtccgt gccaccgacg
     4261 tcgacgcgat gtgggaggcg cacaccaaca tcgccaaccg cggggcattc ttcccgctgg
     4321 gtgtcacgtc ggagtccgca ttccgccgtg agttcggcga gaacggcttc tggcagcgcg
     4381 aggaggggtg gctgctcatc atcacgccgg acggcgagat ggcggggcac atcgagttct
     4441 tcaagccggt caactactgg gactgcttcg agctgtcata tcagctctac gacgaccgtt
     4501 tctccggtcg gggctacgtg agcgaggcgg tgcagctggt ggtcgactac ctgttcgcga
     4561 ccaagaagca gcaccgcatc cagctggtga tcgttcccga gaacggtgcg tcgcgacgga
     4621 tcgccgagaa gtgcgg
//
LOCUS       DATACA010000248         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2442416, whole genome shotgun sequence.
ACCESSION   DATACA010000248 DATACA010000000
VERSION     DATACA010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442416"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..636
                     /gene="atpF"
                     /locus_tag="VHK28_05180"
     CDS             79..636
                     /gene="atpF"
                     /locus_tag="VHK28_05180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX2141654.1"
                     /translation="MLEFFNAFGVDALKLAFQVVNFLLVLWLLNKFLFSRVLERLDER
                     RTRIEKGLEDAEAAARDRELARAEREAAVAEARREAQAMVARATKMADDTRNEILAAA
                     RADAEKATARAREEINAEKEKALAELRTHVAELALDAAGKLVRSEMTAATHRRLVEEF
                     LTEVEPKGRASGASGATRASGGGNG"
     gene            640..1179
                     /gene="atpH"
                     /locus_tag="VHK28_05185"
     CDS             640..1179
                     /gene="atpH"
                     /locus_tag="VHK28_05185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX2141655.1"
                     /translation="MARRETAARRYAEAAFEIGRSDGTLDRWERDMDVLADVLKHAEL
                     RRAVEHPALAYGAKEKLLRRVVGEAVSVEAMNLLLLMIRRGRPRAFDRAVEHFGELLR
                     RHRGVRRAEVRTALPLDDEQRVAVQQRLTELTDARIEMNEIVDESLIGGVAVRIGDVL
                     YDASVRSRLERLRARLTAG"
     gene            1210..>1853
                     /locus_tag="VHK28_05190"
     CDS             1210..>1853
                     /locus_tag="VHK28_05190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393857.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX2141656.1"
                     /translation="MAIRSDEITSIIRSEIKGFDEGADVAGVGTIVEVGDGIAQIYGL
                     SGCLASELLEFPNGIMGMAFNLEEETVGALVLGDYTGLKEGDQVKTTGRVVEVPVGEA
                     LIGRVVNPLGEPIDGKGPIEAKKTRPVERIAPGVIVRQPVDTPVQTGIKAIDAMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGQDLICIYVAIGQKQSTVAQVVAQLEER"
BASE COUNT          311 a          599 c          700 g          243 t
ORIGIN      
        1 atcgcgctga cggaggccat cggcatctac gcgctggtca tcggcctgct cctgatcttc
       61 gtctagggag gccgagcgtt gctcgagttc ttcaacgcct tcggcgtcga cgccctgaag
      121 ctcgccttcc aggtcgtcaa cttcctgctg gtcctgtggc tgctgaacaa gttcctgttc
      181 agccgcgtgc tggagcggct ggacgagcgg aggaccagga tcgagaaggg cctggaggat
      241 gccgaggcgg ccgcccggga ccgggagctg gcccgcgccg agcgcgaggc ggcggtggcc
      301 gaggcgcgcc gcgaggcgca ggccatggtt gcccgcgcca ccaagatggc ggacgacacc
      361 cgcaacgaga tcctggccgc cgcgcgggcc gatgccgaga aggcgaccgc ccgggcgcgc
      421 gaggagatca atgccgagaa ggagaaggcg ctggccgagc tgcgcacgca cgtggccgag
      481 cttgcgctgg acgcggccgg caagctggtg cgctccgaga tgacggccgc cacccatcgg
      541 cgcctggtcg aggagttcct gaccgaggtg gagccgaagg gccgagccag cggcgcatcc
      601 ggcgcgaccc gcgcatctgg cggagggaac ggctagccca tggcgcgacg cgagaccgcg
      661 gcccggcgct acgcggaggc ggcctttgag atcggccgct ccgacggaac gctggatcgc
      721 tgggagcggg acatggacgt cctggccgac gtgctgaagc acgccgagct gcggcgtgcc
      781 gtggagcacc ccgccctggc gtacggagcc aaggagaagc tgctgcgccg cgtggtgggc
      841 gaggcggtca gcgttgaggc catgaacctg ctgctgctga tgatccgccg cggacggccc
      901 cgcgccttcg accgcgcggt cgagcacttc ggggagctgc tgcggcggca tcgcggcgtg
      961 cgccgcgcgg aagtccgaac cgcgctgccg ctggacgacg agcagcgcgt cgccgtccag
     1021 cagcggctga cggagctgac cgacgccagg atcgagatga acgagattgt ggacgagtcc
     1081 ctgatcggcg gcgtggccgt gcggatcggc gacgtgctgt atgacgcgag cgtgcggagc
     1141 cgcctggagc ggctgcgtgc gcggctgacc gccgggtaaa gccggcagag ccgggtagag
     1201 aggaagtaca tggcgattcg atccgacgag atcacgtcga tcatcaggag cgagatcaag
     1261 ggcttcgacg agggcgccga cgtcgccggc gtcggcacca tcgtggaggt cggcgacggc
     1321 atcgcgcaga tctacggcct ctccggctgc ctggccagcg agctgctcga gttccccaac
     1381 gggatcatgg gcatggcctt caacctggag gaggagaccg tcggcgccct ggtcctgggc
     1441 gactacaccg gcctcaagga gggggaccag gtcaagacca cgggtcgcgt ggtcgaggtg
     1501 ccggtcggcg aggccctgat cgggcgcgtg gtcaaccccc tcggcgagcc catcgacggc
     1561 aaggggccca tcgaggccaa gaagacacgg ccggtggagc gcatcgcccc gggcgtcatc
     1621 gtgcgccagc cggtcgacac cccggtccag accggcatca aggccatcga tgccatgatc
     1681 cccattgggc gcggacagcg cgagctgatc atcggtgacc ggcagacggg caagacggcc
     1741 atcgcggtcg acaccatcat caaccagaag ggccaggacc tgatctgcat ctacgtcgcc
     1801 atcggccaga agcagtcgac ggtggcgcag gtggtggccc agctggagga gcg
//
LOCUS       DATACA010000249         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2844929, whole genome shotgun sequence.
ACCESSION   DATACA010000249 DATACA010000000
VERSION     DATACA010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2844929"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="rfaE2"
                     /locus_tag="VHK28_05195"
     CDS             <1..657
                     /gene="rfaE2"
                     /locus_tag="VHK28_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX2141657.1"
                     /translation="FAACLALAMAAGIEPPTGVELASAAAAVAVGKRGTATCTVDELL
                     ATVSGGPVAPDREALARRVALEKRRGRRIVFTNGCFDILHSGHVAYLEQARALGDVLV
                     VAVNGDESVRRLKGPGRPVNALPDRLEVLSALASVDYVVSFDEDSPQALIEALRPDVV
                     AKGGDYSEETLPEAELVRGMGGEVRILPFVADRSTTRIIERIGARHEPTPAGVGPGAS
                     "
     gene            654..>1616
                     /locus_tag="VHK28_05200"
     CDS             654..>1616
                     /locus_tag="VHK28_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2141658.1"
                     /translation="MNRQWQAADRILAVRLDSMGDVLMTEPALAALRANGRRHVTLLT
                     SASAAPIARLLPSVDAVLTYPAPWIKATERRDPGLDLMMVERLRAGRFDGAVIFTVYS
                     QSSLPAALTCHLAGIPLRVARSRENPYLLLTDWVREDEPDVERHEVERQLSLVRGLGF
                     VPTRDRMCLRLKPAHRSQALRALLEAGVAMDRPWLVLHPGASAPSRRYPTESYARVAD
                     RLVLEDDWQVVLLSGPDDEDLAEEVRSVMRAPTASVGGLSVGEMAAVIAAAPLFVGNN
                     SGPMHVASAVGTPVVVPYAQTNPQHTPWNVPARVLTRDVPCRNCY"
BASE COUNT          210 a          576 c          587 g          243 t
ORIGIN      
        1 ttcgccgcct gcctggcgct ggccatggcg gccggcatcg agccgccgac cggggtggag
       61 ctggcatccg ccgccgctgc ggtggcggtt ggcaagcgcg ggacggcgac ctgcaccgtc
      121 gacgagctgc tggccacggt ctccggcggc ccggtggcac ccgatcgcga ggccctggcc
      181 cgccgggtgg cgctcgagaa gcggcgtggg cgccgcatcg tgttcaccaa cggatgcttc
      241 gacatcctgc attccgggca cgtggcctac ctcgaacagg cccgggcgct gggcgacgtg
      301 cttgtggtgg ccgtcaacgg cgacgagagc gtgcgccgcc tgaaggggcc gggccggccg
      361 gtcaacgccc tccccgaccg tctcgaggtg ctctccgcgc tggccagcgt ggactacgtg
      421 gtctccttcg acgaggactc cccgcaggca ttgatcgagg cgctgcgccc cgacgtggtg
      481 gccaagggcg gtgactactc ggaggagacg ctccccgagg ccgagctggt gcggggcatg
      541 ggcggagagg tgcgcatcct gccgttcgtc gcggatcggt ccacgacgcg catcatcgag
      601 cgcatcggtg cgcggcacga gccgaccccg gccggcgtcg gccccggcgc ctcatgaacc
      661 ggcagtggca ggccgccgat cgcatcctgg ccgttcgcct ggattcgatg ggcgatgtcc
      721 tgatgaccga gccggcattg gccgcactgc gcgccaacgg ccgtcgccat gtcacgctgc
      781 tcacctccgc cagcgcggcg ccgatcgccc gcctcctccc gtccgtcgat gcggtgctca
      841 cctatcccgc gccgtggatc aaggctaccg agcggcgcga cccggggctg gacctgatga
      901 tggtcgagcg gctgcgggcc gggcgattcg acggggccgt gatcttcacc gtctacagcc
      961 agagctcgct gccggcggcg ctcacctgcc acctggcggg catcccactg cgcgtggcgc
     1021 gctcccgcga gaacccgtac ctcctgctga ccgactgggt gcgcgaagac gagccggacg
     1081 tggagcgcca cgaggtcgaa cggcagctga gcctggtgcg cggcctgggc ttcgtgccga
     1141 ctcgtgaccg catgtgcctg cgcctgaagc cggcacatcg gtcccaggct ctccgcgcgc
     1201 tgctcgaggc gggagtcgcg atggaccgcc cgtggctggt gctgcacccg ggcgcgagcg
     1261 cgccgtcgcg ccgctaccca acggagtcgt acgcgcgtgt cgccgaccgg ttggtgctcg
     1321 aggatgattg gcaggtcgtc ctgctgagtg gcccggatga cgaggacctc gccgaggagg
     1381 tgcgcagcgt catgcgcgca ccgacagcat cggttggtgg cctatcggtg ggcgagatgg
     1441 cggcggtgat cgcagcggcc ccgctgttcg tggggaacaa cagcgggccc atgcacgtgg
     1501 cctccgcggt cggcacgccc gttgtcgtgc cctacgccca gacgaacccg cagcacactc
     1561 cctggaacgt gcccgcacgg gtcctgaccc gtgacgttcc gtgtcgcaac tgctac
//
LOCUS       DATACA010000250         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1399095, whole genome shotgun sequence.
ACCESSION   DATACA010000250 DATACA010000000
VERSION     DATACA010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399095"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..770)
                     /locus_tag="VHK28_05205"
     CDS             complement(12..770)
                     /locus_tag="VHK28_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX2141659.1"
                     /translation="MLRALKRWWKYLGAKLTSSFNEKADPKVQLEQAITEAQDQHRRL
                     REQAANVIANQKQTEMRLDRAMEELEKVSANARQAVLMADEAQKAGDTAKVEEYTSAA
                     ESFATRLIAIEGEVEDLKQLALQSSQASDQAKAAVQQNATALQRKLAERNKLMSQLDQ
                     AKMQEQVNSAMASLSETVGDDVPTFNEVRDKIEARYAKAKGTAELTESSVETRMLEVE
                     QAAANSEAQNRLAQIRSQLGIGPAASSGDAGPTA"
     gene            complement(780..>1943)
                     /locus_tag="VHK28_05210"
     CDS             complement(780..>1943)
                     /locus_tag="VHK28_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2141660.1"
                     /translation="AAGAVTARPGRQLRGSDRHAEEVVADVGAGIAGPVTGEVLVQLP
                     AVRRLVRGLRLSLDLRSRNVRDVLRRFAPVVMGRGVVQLLTYVELVLASLLAVGAVSA
                     LTYAQVLYLLPISLFGMAVAAAELPELSRLGRAGADAIRARLVGGMERITFYVAPTVA
                     IYVFAGDVVVGALLQRGEFDAADTRVVWLVLAAFALGLLATTRSRLLQNGLYALDRSR
                     LVARIAVARVALAAALGALLMFPLDRLAVVGSSIERRGDLGFSPLTDAVRLVQDAPPR
                     LGAVGLALGAAISSWVEYRLLRGALEWRIGKLPAMASGARWSLVAAAAAGILAAGLRA
                     VSDGLPSLVAVVLVCGPAAAAYLAITATVGVPEASALLQRVRPGAAEGARGQG"
BASE COUNT          275 a          700 c          703 g          265 t
ORIGIN      
        1 cgccgacctc gtcaggcggt gggaccggcg tcacccgacg atgcggcggg gccgatgccg
       61 agctgggacc ggatctgggc caggcggttc tgcgcctcgc tgttggcggc cgcctgctcg
      121 acctccagca tgcgggtctc caccgagctc tcggtgagct ccgcggtgcc cttggccttg
      181 gcgtagcgag cctcgatctt gtcgcggacc tcgttgaacg tcggcacgtc gtcgcccacg
      241 gtctccgaca gcgacgccat cgccgagttg acctgctcct gcatcttggc ctggtcgagc
      301 tgcgacatga gcttgttgcg ctcggccagc ttgcgctgca gcgcggtggc gttctgctgc
      361 acggcggcct tggcctggtc ggacgcctgc gacgactgca gcgccagctg cttgaggtcc
      421 tccacctcgc cttcgatggc gatgaggcgg gtggcgaagg actccgcggc ggacgtgtac
      481 tcctcgacct tggcggtgtc gccggccttc tgcgcctcgt cggccatcag cacggcttgg
      541 cgagcgttgg ccgaaacctt ctccagctcc tccatggccc ggtcgaggcg catctcggtc
      601 tgcttctggt tggcgatgac gttggccgcc tgctcccgca gccggcggtg ctggtcctgg
      661 gcctcggtga tcgcctgttc gagctggacc ttcgggtcgg ccttctcgtt gaacgacgag
      721 gtcagcttgg ccccgaggta cttccaccag cgcttgaggg ctctgagcat gcgacgagcc
      781 taaccctgcc cccgggctcc ttccgccgcg ccgggccgga cccgctggag cagcgcactg
      841 gcctccggca cgccgaccgt ggcggtgatg gcgaggtagg cggcggcggc cggcccgcag
      901 acgaggacca ctgcgacgag gctcgggagg ccgtcgctca ccgcccggag gcccgccgcc
      961 aggatgccgg ccgcggcagc ggcgaccagc gaccaccggg caccgctggc catcgccggc
     1021 agcttgccga tccgccactc gagcgcgccg cgcagcaacc ggtactcgac ccaggacgag
     1081 atggcggcac cgagggccag gccgaccgca cccagccggg gaggtgcatc ctgcaccagg
     1141 cggaccgcat cggtgagcgg ggagaagccg aggtcgccgc ggcgctcgat cgacgatccc
     1201 accacggcca gccggtcgag cgggaacatg agcagcgcgc cgagcgccgc ggcgagcgcg
     1261 acgcgagcca cggcgatgcg ggcgacgagc cgggaccggt cgagcgcgta caggccgttc
     1321 tgcagcaggc gggagcgagt cgtggccagg agcccgagcg cgaacgccgc gagcacgagc
     1381 cagaccaccc gggtgtccgc ggcgtcgaac tcgccccgct ggagcagcgc tccgacgacg
     1441 acgtcgcccg cgaagacgta gatggccacc gtcggcgcga cgtagaaggt gatccgttcc
     1501 atgcccccga ccaggcgggc gcggatcgcg tcggcgccgg cccggccgag ccgtgacagc
     1561 tccggcagct ccgcggcagc gaccgccatg ccgaagaggc tgatgggcag caggtagagc
     1621 acctgtgcgt aggtgagcgc ggacacggcg ccgacggcga gcaggctggc gaggacgagc
     1681 tccacgtagg tcaggagctg cacgaccccg cgccccatca cgaccggcgc gaagcgtcgg
     1741 aggacatcgc gcacgttccg ggatcgcagg tccagtgaca ggcgaagtcc tcggaccagt
     1801 cgccgcaccg ccgggagctg gacgaggact tcgcctgtca ctggacctgc gatcccggcg
     1861 ccgacgtcag ccaccacctc ctcggcatgg cggtcgctgc cgcggagctg ccggcccggc
     1921 cgagccgtga cagctccggc agc
//
LOCUS       DATACA010000251         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1478597, whole genome shotgun sequence.
ACCESSION   DATACA010000251 DATACA010000000
VERSION     DATACA010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478597"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VHK28_05215"
     CDS             <1..704
                     /locus_tag="VHK28_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX2141661.1"
                     /translation="REIELVVDRYEGALPTNAWPNWVLGNHDLPRVASRIGAGQARVA
                     AMLLLSLRGTPTLYYGDELGMEETPVPPEDVQDVREINQPGRGLGRDPCRTPMPWDGS
                     PGAGFTTGRPWLPIGEDNRQRNVEASRSDPDSMLSLYRRLLELRRREPALAIGSYRPV
                     PADGSVLAFEREQDGRRLLVTLNLSDQPSRLELEPDGRRYRPLLSTLPGAAHDQVGPA
                     IDLGPDEGLILEPRE"
     gene            746..1933
                     /gene="chrA"
                     /locus_tag="VHK28_05220"
     CDS             746..1933
                     /gene="chrA"
                     /locus_tag="VHK28_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX2141662.1"
                     /translation="MDDPPAAQQGSWRSVLGVALQLGLSSFGGPIAHLGYFHREYVER
                     RRWLDERDFADLVALCQTLPGPASSQLGIAIGRLRAGVAGALAAWVGFTLPSAVIMVG
                     FALLAGSTDLTDAGWVAGLKLAAVAVVAHAVLRMSATLAPKLAHRLLALVYAGVLLIL
                     EGPIIQVGVLAAAALLGYAWTRGGRRGGPTARLEGRKSGVAALAVFGGLLVALPLLRA
                     VDGQVVALVDTFYRAGSLVFGGGHVVLPLLDAGVVGPGWVGEDQFLAGYGLAQAIPGP
                     LLTFSAYLGAVAAPEPNGVLGAGLALAAIFLPSFLLVFGVLPFWEPLRGSPRVRGSLD
                     AVNVAVIGLLLAALYDPVTTAAVDSPLDAAVALGGLALLSLRLPPLAVVAAAAAGGQL
                     TGA"
BASE COUNT          251 a          760 c          760 g          322 t
ORIGIN      
        1 cgcgtgagat cgagctggtg gtggaccgat acgagggcgc ccttccgacc aacgcctggc
       61 cgaactgggt gctgggcaac cacgaccttc cccgggtggc cagccgcatc ggtgccggac
      121 aggcccgcgt cgcggccatg ctgctgctct ctctgcgcgg cacgccgacc ctctactacg
      181 gcgacgagct gggcatggag gagacgccgg tgccgccaga ggacgtccag gacgtacgtg
      241 agatcaacca gcccggccga ggcctggggc gtgacccgtg ccgcacgccc atgccctggg
      301 atggttctcc cggtgccggg ttcacgaccg ggcgaccctg gctgccgatc ggcgaggaca
      361 accgacagcg gaacgtggag gcctcgcgat ccgacccgga ttcgatgctc tccctctacc
      421 gacgcctcct cgagctccgg cggcgggagc cggccctggc catcggcagc taccgcccgg
      481 tcccggccga cggatcggtc ctggcgttcg agcgcgagca ggacggccgg cgcctcctgg
      541 tgacgctcaa cctctccgac cagccctccc gcctggagct ggagcccgat gggcgccgct
      601 accgtcccct gctctccacc ctccccggtg ctgcccacga ccaggtcggg ccggcgatcg
      661 acctggggcc ggacgagggg ctcatcctgg agccgcgcga gtagacgagc ccggcagcga
      721 gccgccacgt ggcaagatcg ggcggatgga cgatccgccc gccgcgcagc agggcagctg
      781 gcgttcggtg ctcggcgtgg cgctacagct gggcctgagc tcgttcggcg ggccgatcgc
      841 gcacctcggc tacttccacc gcgagtacgt ggagcgacgg cggtggctgg acgagcggga
      901 cttcgccgac ctggtggccc tgtgccagac gttgcccggt ccggccagca gccagctcgg
      961 aatcgccatc ggcaggctgc gtgccggagt ggccggcgcg ctggccgcct gggtcggctt
     1021 caccctgcca tcggcggtga tcatggtcgg cttcgccctg ctggctggca gcactgacct
     1081 gaccgatgcc ggctgggtgg cggggctgaa gctcgcggcg gtggccgtgg tcgcgcatgc
     1141 cgtgctgcgg atgtcggcga ccctcgcgcc aaagctggcg caccggctcc tggcgctggt
     1201 gtacgccggc gtcctgctca tcctcgaggg cccgatcatc caggttggcg tgctggctgc
     1261 cgcggcgctg ctcggatatg cctggacgcg aggcgggcgg cgaggtggac ccacggctcg
     1321 cctggaaggc cgcaagtcgg gcgtggccgc gctggccgtc ttcggtggac tgctcgtcgc
     1381 gctgccactg ctccgggcgg tcgacggcca ggtcgtcgcg ctggtcgaca ccttctaccg
     1441 cgccgggtcg ctggtcttcg gcggcggtca tgtggtactg cccctgctcg acgccggggt
     1501 cgtcgggccg ggctgggtcg gcgaggacca gttcctggca gggtacggac tggcacaggc
     1561 gatccccggg ccgctcctca ccttctcggc ctacctcggg gcggtggccg cgccggaacc
     1621 caacggggta ctgggagccg gcctggcact ggccgccatc ttcctgcctt cgttcctgct
     1681 ggtcttcggc gtcctgccct tctgggagcc tctgcgaggc tcgccgcgag tgcgcggctc
     1741 cctggatgcg gtgaacgtgg ccgtcatcgg gctgctgctg gctgccctgt acgacccggt
     1801 gacgaccgct gcagttgact cgccgctcga cgcggccgtg gcgctcggcg gcctggcgct
     1861 gctgagcctg cggctgcctc ccctggccgt cgtggccgcg gcggccgccg ggggccagct
     1921 caccggagct tgagctcgtg gcaccctcga tgcagtcccg gtgcgctccc ggctaccgtg
     1981 atcacgcgta caagccaaaa acgggagcgc cgcgtgccag accagcacca ggaccatcag
     2041 gctcacgacg agcacacggg gccgccgcca gccgatccgc atcgggcgcc gct
//
LOCUS       DATACA010000252         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2683803, whole genome shotgun sequence.
ACCESSION   DATACA010000252 DATACA010000000
VERSION     DATACA010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683803"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="VHK28_05225"
     CDS             <1..1817
                     /locus_tag="VHK28_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2141663.1"
                     /translation="ILARDPNPVAAICGYVCSAPCEKACRRADIDKPLAIRAMKRFLV
                     DWHYANNMPDNIVVGPSTGRSVAVIGAGPAGLSVAKELATYGHDVHVYEALPSGGGTT
                     LIGVPAFRLPRDVIELDVNWVAKHGVEFHYNVEIGRDITIDQLKERHDAVVVATGCMY
                     PVAMNVPGEELDGVIYGVDFLKRANLGEEQWVGEHVVVVGGGYTAMDSSRTALRLGAK
                     TSTIVYRRGPDEMVVDEEEQHETRFEGVEFEFFASPVEVVSDENGKVSGITFQRTRLG
                     PPDASGRRSAEPIPGSEFTIACDMVIPCTSQSADNNVLGEFGSKLNRHLGVTDTRNVV
                     PGGSVSSWRTPPMNGSTPSSPDGGRHNQLPFDFNRGYVTTDADTFATSIDGVFAVGDF
                     VTGPATIIEAAGRGRRAARAVDRWLAGVRGEELTELPVINRAVVTQALDHDMPRDYEA
                     IHRQEIPMAPPELRRDFTSLVEVGYDTRSAIDEGARCLQCNHNINIDGPRCILCGLCA
                     DVCPEGVIYMIDKAQIGGDDPEIDLFQSWPRGIAMIIDEERCIRCNLCVERCPTNCIT
                     MDRLELERFTPDGKVLLESYKDNVLGQIGQQAGMAVDR"
BASE COUNT          307 a          619 c          622 g          278 t
ORIGIN      
        1 acatcctggc ccgcgacccg aacccggtgg ccgccatctg tggctacgtg tgcagcgccc
       61 cgtgcgagaa ggcctgccgg cgggccgaca tcgacaagcc gctggccatc cgcgccatga
      121 agcgcttcct ggtggactgg cactacgcca acaacatgcc ggacaacatc gtggtcggcc
      181 cgtccaccgg acgcagcgtg gcggtcatcg gtgcaggccc ggcggggctg agcgtggcca
      241 aggagctggc cacctacggt cacgacgtcc acgtctacga ggcgctgccg tcgggcggcg
      301 gcaccacgct catcggcgtg cccgccttcc ggctgccgcg cgacgtgatc gagctggatg
      361 tcaactgggt cgccaagcac ggcgtcgagt tccactacaa cgtcgagatc gggcgcgaca
      421 tcaccatcga ccagctcaag gagcggcacg acgcggtggt ggtggccacc ggctgcatgt
      481 acccggtggc catgaacgtg ccgggcgagg agctggacgg tgtgatctac ggcgtcgact
      541 tcctcaagcg ggccaacctc ggcgaggagc agtgggttgg cgagcacgtg gtggtggtcg
      601 gtggcggcta caccgcgatg gactcctcgc gcacggcgct gcgcctggga gccaagacca
      661 gcaccatcgt ctaccgccgc ggtccggatg agatggtggt ggacgaggag gagcagcacg
      721 agacccgctt cgagggcgtg gagttcgagt tcttcgcctc cccggtggag gtcgtcagcg
      781 acgagaacgg caaggtctcc ggcatcacct tccagcgcac gcgcctgggc ccgccagacg
      841 ccagcggccg ccgcagcgcc gagcccattc ccggcagcga gttcaccatc gcctgcgaca
      901 tggtcatccc ctgcaccagc cagtcggcgg acaacaacgt gctcggcgag ttcggctcca
      961 agctgaaccg ccacctgggg gtgaccgata ctcgcaacgt cgtgccgggc ggatcggtct
     1021 cctcgtggcg cacgccgccc atgaacggca gtactccgtc cagcccggat ggcggccgtc
     1081 acaaccagct gcccttcgac ttcaaccgtg gctacgtgac gacggacgcg gacaccttcg
     1141 ccaccagcat cgatggcgtc ttcgctgtcg gtgacttcgt gaccggcccg gcgaccatca
     1201 tcgaggcggc cggtcgcggc cggcgggcgg cacgcgccgt ggaccgctgg ctggccgggg
     1261 tgcgcggcga ggagcttacc gagctgccgg tcatcaaccg ggctgtcgtg acccaggctt
     1321 tggaccacga catgccccgt gactacgagg ccatccaccg gcaggagatc cccatggctc
     1381 cgccggagct gcggcgcgac ttcaccagcc tggtggaggt cggctacgac acgcgctcgg
     1441 cgatcgacga gggcgctcgc tgcctccagt gcaaccacaa catcaacatc gatggtccgc
     1501 gctgtatcct gtgcgggctc tgcgccgatg tctgccccga aggcgtgatc tacatgatcg
     1561 acaaggcgca gatcggcggc gatgacccgg agatcgacct gttccagtcg tggccgcgcg
     1621 ggattgccat gatcatcgac gaggagcgct gcatccgctg caacctctgc gtcgagcggt
     1681 gtccgaccaa ctgcatcacc atggaccggc tcgagctcga gcggttcacg cctgacggga
     1741 aggtgctctt ggagagctac aaggacaacg tgctgggtca gatcggccag caggccggga
     1801 tggcggtgga ccgatgagct tcctgg
//
LOCUS       DATACA010000253         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2845118, whole genome shotgun sequence.
ACCESSION   DATACA010000253 DATACA010000000
VERSION     DATACA010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2845118"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHK28_05230"
     CDS             complement(<1..219)
                     /locus_tag="VHK28_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141664.1"
                     /translation="MALQLAIVVADMAMSDLAQVQEIERLSFSTPWPAYAFEQELRGN
                     RLARYVVARAEVPEGERVVGFAGVWLMVD"
     gene            complement(219..851)
                     /gene="tsaB"
                     /locus_tag="VHK28_05235"
     CDS             complement(219..851)
                     /gene="tsaB"
                     /locus_tag="VHK28_05235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX2141665.1"
                     /translation="MILAIESASTDLSVALASPDGALLGTDGWTAGRRQGGELMPRLL
                     ALLERHGAGLEQLTAVAVGTGPGSFTGLRVGLSLAKGLALALRIPIAGVPSLESWLAA
                     EPGALAAIGRAGAGEAYLLLRDQDQPRIVDRDELPTDVKSSHVVAPVELAEALGLAAA
                     VAPHRAASAVARQAVARMAIDPAGDDLAPLEPAYLRAPRGIGPLPEQEAG"
     gene            complement(848..1342)
                     /gene="tsaE"
                     /locus_tag="VHK28_05240"
     CDS             complement(848..1342)
                     /gene="tsaE"
                     /locus_tag="VHK28_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX2141666.1"
                     /translation="MDPSTAAAPVAGTFTSRSPDETRAFGRRVGRVAEPGTLLALSGP
                     LGAGKTQLAKGVAEGLGVRGVVNSPTFVLMNEHEGRLRLFHVDAYRLGDPEEALAAGL
                     LDERQAAGVTVIEWADRLRAWLPPERLEISLEPDPDPEGRTVRWTAIGERHAALAAEI
                     MSGS"
     gene            complement(1361..2419)
                     /locus_tag="VHK28_05245"
     CDS             complement(1361..2419)
                     /locus_tag="VHK28_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141667.1"
                     /translation="MSFDDAPAPTLHRVRIPRGAVPAVRVGSDVRPDQVLATLRRPGA
                     PVRVPLAAVLRRRPEEVVGLLTVRPGASVAEGEPIAGEPGGRVATAPIGGLLLGVSAT
                     DGSALLAPLGAEESVIGHVRGTVRGVDPASITVEVPAVLVRGVGGSGEAVHGELVVAV
                     HEAADELRAAAIDVSATERIVVGGSRASAETLTRARAMGVAGIVLGGVLDKELRDFEA
                     IQRRRREIGGLSGSFGVMLLEGYGKVGIDPQRFAWLRAHAGRMASLFGGEGLLYVYDA
                     QPMQARRALPRVGERVIAHRRPFQGRTGVLVAELEDLHATPSGVPSRMGLVRFEDGRL
                     APVPLANLEATLPVAQGR"
BASE COUNT          356 a         1027 c          879 g          287 t
ORIGIN      
        1 gtccaccatc agccacacgc cggcgaagcc gaccacccgc tcgccctcgg gcacctccgc
       61 ccgcgccacc acgtagcgcg ccagccggtt gccgcgcagc tcctgctcga aggcgtaagc
      121 cggccacggg gtgctgaacg agagccgctc gatctcctgc acctgcgcca ggtcggacat
      181 ggccatgtca gcgacgacga tcgccagctg gagcgccatc agcccgcctc ctgctccggc
      241 aatggaccga tgcctcgcgg ggcgcgcagg taggccggct cgagaggagc caggtcgtca
      301 cctgccgggt cgatggccat tcgcgccacc gcctgcctgg ccacggcaga cgccgcccga
      361 tgcggcgcca ccgcggcggc cagccccaac gcctcggcga gctccacggg tgccactacg
      421 tggctgctct tgacatcggt cggcagctcg tcacggtcga cgatccgcgg ctggtcctgg
      481 tcgcgcagga gcaggtacgc ctctcctgcg ccggcccgcc cgatggccgc cagcgctccc
      541 ggctcggccg cgagccagga ttcgaggctc ggcaccccgg cgatcgggat gcgcagggcg
      601 agcgccagtc ccttggccag gctgaggccg acccgcagcc cggtgaacga gccgggcccg
      661 gtcccgaccg ccactgcggt cagctgctcg agcccggcac cgtggcgctc gaggagcgcc
      721 agcaggcgcg gcatgagctc accgccctgg cgccgtccag ccgtccagcc gtcggtcccc
      781 agcagcgccc catccgggct ggccagcgcc acggacaggt cggtggacgc cgactcgatg
      841 gccaggatca tgagcccgac atgatctcgg cggccagggc cgcgtgccgc tcgccgattg
      901 ccgtccagcg caccgtccgc ccctccgggt cgggatccgg ctccaggcta atctccagcc
      961 gctccggcgg aagccaggcg cggaggcggt cggcccactc gatcacggtg acgccggcgg
     1021 cctgccgctc gtccagcagc ccggccgcca gcgcctcctc tgggtcaccg agccggtatg
     1081 catccacgtg gaagaggcgc agccggccct cgtgctcgtt catcagcacg aaggtcgggc
     1141 tgttgaccac gccgcgcacg cccaggccct cggccactcc cttggccagc tgcgtcttgc
     1201 ccgcacccag cgggccggac agcgcaagca acgtcccggg ctccgcgacg cggccgaccc
     1261 ggcgtccgaa ggcccgggtc tcgtccggcg agcggctggt gaacgtgccc gcgaccgggg
     1321 ccgccgcagt ggagggatcc atgtgccaag gataggggcg tcagcgaccc tgagcgaccg
     1381 gcagcgtggc ctccaggttg gcgagcggaa caggcgccag ccggccgtcc tcgaagcgga
     1441 ccaggcccat ccggctgggc acgccggacg gggtggcgtg cagatcctcc agctcggcga
     1501 ccagcacgcc ggtgcgcccc tggaacgggc gccggtgggc gatcacccgc tccccgaccc
     1561 tgggcagggc gcggcgggcc tgcatgggtt gcgcatcgta aacgtagagc agcccttcgc
     1621 caccgaacag gctggccatg cggccggcgt gcgcccgcag ccaggcgaac cgctgcgggt
     1681 cgatgcccac cttgccgtac ccctccaaaa gcatgacccc gaacgagccg gagaggccgc
     1741 cgatctcgcg ccggcggcgc tggatggcct cgaagtcgcg cagctccttg tccagcaccc
     1801 caccgaggac gatgccggcc acgcccatgg cccgcgcccg ggtcagcgtc tcggcggatg
     1861 cccgcgaccc gccgaccacg atgcgctcgg tggccgacac gtcgatggcc gccgcccgca
     1921 gctcgtccgc ggcctcgtgc acggccacca ccagctcgcc gtggaccgcc tcgccgctgc
     1981 cgcccacgcc gcgcaccagc acagccggga cctcgaccgt gatcgacgct gggtcgactc
     2041 cgcgcaccgt cccgcgcaca tgaccgatga ccgactcctc ggcccccagc ggagccagca
     2101 gcgcgctgcc gtcggtcgcc gagacgccca gcagcagccc gccgatgggc gccgtggcca
     2161 cacgcccgcc cggctcgccg gcaatcggct cgccctcggc gaccgatgcg ccggggcgca
     2221 cggtgagcag gccgaccacc tcctccggac ggcgccgcag cacggcggcc agcggcaccc
     2281 gaaccggagc gcctggccgc cgcagcgtgg cgagcacctg gtccggacgg acgtcgctgc
     2341 ccacgcggac ggccggcacc gcgccacgcg gtatgcgaac ccgatgcagc gttggggcgg
     2401 gggcgtcgtc aaagctcacg gcgccctcca gccggccggc atggccgctt cccggtggaa
     2461 cgtctcccgc cacccggcca gcaccgcgcg gcggtcctcc gagcggcgcg gcatggccag
     2521 cggcacgtcg cgggcgtcga gcatcagac
//
LOCUS       DATACA010000254         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1478726, whole genome shotgun sequence.
ACCESSION   DATACA010000254 DATACA010000000
VERSION     DATACA010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478726"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..930)
                     /locus_tag="VHK28_05250"
     CDS             complement(121..930)
                     /locus_tag="VHK28_05250"
                     /inference="COORDINATES: protein motif:HMM:NF018758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA methyltransferase"
                     /protein_id="HEX2141668.1"
                     /translation="MTEEVTARVRRSARYRDVDPELVARLAIEELPRARNADEATKRV
                     KRRLHQAVGAFRGASDAGLLAHLRQAWTGSLRADVFRDACRAALASHASTRERLPVLA
                     DFYARTWERTGGPPSRLADLGCGLNPLALPWMGLLPETHYLAVEADRRPLELVSGFLD
                     LVDQPHEVRSTDLVTDPPPDEVDVALLLKLVPTLDQQDPEAATRLLGAIRARHAVVTF
                     PAASLGGRGKGMQRTYRARFDRLVAELGPRVMDVAEASVPGELIFVLELAA"
     gene            complement(927..1790)
                     /gene="rsmI"
                     /locus_tag="VHK28_05255"
     CDS             complement(927..1790)
                     /gene="rsmI"
                     /locus_tag="VHK28_05255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX2141669.1"
                     /translation="MERSGTLYLVPVPIGNDEDITLRALRCLEEVDVVAAEDTRDFGR
                     LARRHGIRTPSLSYHDHNERQRAPELLRRLQEGKSVALVSDAGTPLFSDPGYRLARAA
                     AETGIGLVALPGASAVTTALAASGLPPIPFRFCGFPPRTGAARRAWFETLAHDDATLV
                     LFEAPHRLLGCVEDALRVLGDREACLARNLTKPHEALQRGTLAGLATRLSEEGEVRGE
                     VTVVIAGSPAGSAGVGAAAAERSAALLLRAGVARRVVSELLTTIHGLRRREAYDLILR
                     TLADQGLDREP"
     gene            1935..2333
                     /locus_tag="VHK28_05260"
     CDS             1935..2333
                     /locus_tag="VHK28_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141670.1"
                     /translation="MARTGVGTDVPRVLIVEDDAALRDIYAGALSGFGHDVRAAAEGG
                     EALSLLENGWKPCLIFLDLRMPGIDGWELTRRVRADDRWRDIPIVVVAAHFRIDHEAR
                     ELGAAGWLQKPFDLTQLDDYVQTLCNRKAS"
BASE COUNT          351 a          855 c          881 g          345 t
ORIGIN      
        1 gcccctcgat gccgaccacg cggaggtcct cgccggtcag gccgcggacg ccggcgctgg
       61 tggcatcggg taggaagacg ggcgtggcca gctcacccag gcgggttgtg agcgaggtgc
      121 tcatgcggcc agctcgagca cgaagatgag ctcgcccggg accgatgcct ccgccacgtc
      181 catgacccgc ggtccgagct cggccaccag ccggtcgaag cgggcacggt aggtgcgctg
      241 catgcccttg ccccgaccgc cgagcgatgc tgccgggaag gtgacgaccg cgtggcgcgc
      301 gcggatcgcg cccagcagcc gggtggcggc ctccgggtcc tgctggtcga gggtggggac
      361 cagcttgagg aggagggcca cgtcgacctc gtcaggcggt ggatcggtca cgaggtccgt
      421 gctgcgcact tcatgcggtt ggtcgaccag gtcaaggaag ccggacacca gctcgagggg
      481 ccgccgatcc gcctcgaccg ccaggtaatg ggtttcaggc aaaaggccca tccacggcag
      541 ggccagcggg ttcagcccgc agcccaggtc tgccaatcgg ctgggtgggc cgccggttcg
      601 ctcccaggtc ctggcgtaga agtcggccag gaccggcagt cgctcccggg tcgaggcatg
      661 tgaggccagc gcggctcggc acgcgtcgcg gaacacatcg gcccgcaggc ttccggtcca
      721 tgcctggcgc agatgagcga gcaggccagc gtcggaggcc ccgcggaagg cgccgactgc
      781 ctggtgcagc cgccgcttga cccgtttcgt cgcctcatcc gcattgcgtg cgcgcggcag
      841 ctcctcaatc gccagcctgg caaccagctc gggatccacg tcgcggtagc gcgcggagcg
      901 acggacgcgg gcggtcacct cctcggtcat ggctcgcgat ccaagccctg atcggcgagc
      961 gttcgcagga tgaggtcgta cgcctcgcga cggcgaaggc cgtggatggt cgtcagcagc
     1021 tcgctgacca cccggcgcgc cacgccggcg cgcagcagca gcgccgccga acgctcggcc
     1081 gcggccgcgc cgaccccggc gcttccagcc gggctgccgg cgatgaccac ggtcacttcg
     1141 ccgcggacct cgccctcctc gctgagccgt gttgccaggc cggcgagcgt gccgcgctgc
     1201 agggcctcgt gcggcttggt caggttgcgc gccaggcagg cctcgcgatc tcccaggacg
     1261 cgcagcgcgt cctccacgca gccgagcagc cgatgcggcg cctcgaacag caccagcgtc
     1321 gcatcgtcgt gggccagcgt ctcgaaccac gcgcggcgcg ctgcgcccgt ccgcggcggg
     1381 aagccgcaga accgaaaggg gatggggggc aggccggagg cggccagggc ggtggtcacc
     1441 gccgaggcac cgggaagcgc cacgaggccg atccccgtct cggcggcggc gcgcgccagc
     1501 cggtagcccg gatcgctgaa cagcggagtt ccagcgtcgc tgaccagcgc cacggacttg
     1561 ccctcctgca agcgccgcag cagctccggg gcgcgctggc gctcgttgtg gtcgtggtag
     1621 ctgagcgacg gcgtgcggat tccgtgccgt cgagccagcc tgccgaagtc gcgcgtgtcc
     1681 tcggccgcca ccacgtccac ttcctcgagg cagcgcaggg cgcggagggt gatgtcctca
     1741 tcgttgccga tgggcaccgg caccaggtac agggttcccg agcgctccac ggccacaggg
     1801 tagccgagcc cttcaccgcc gccgcgcggt tcgctaggat gcgtgccacg gcacctgcgg
     1861 catgcctcac cgacggcggt cgcgccccga gcggcgtcgt cgatccgact ggctttccag
     1921 cgagggaggc gcacttggca cgcaccggcg tcggcactga tgtcccgcgc gtactcatcg
     1981 tggaggacga cgctgcgctg cgtgacatct acgccggcgc gctgtccggt ttcgggcacg
     2041 acgtccgcgc tgcagcagaa ggcggcgagg cgctctccct gctggagaat ggctggaagc
     2101 cgtgcctcat cttcctcgac ctgcgcatgc ccggcattga cggatgggag ctgacccgcc
     2161 gcgtgcgtgc cgatgatcgc tggagggata tcccgatcgt ggtggtcgcc gcccatttcc
     2221 gtatcgacca cgaggctcgt gagctgggcg ccgccggatg gctgcagaag ccatttgacc
     2281 tcacgcagct ggacgattac gtccagacac tgtgcaaccg gaaggcgtca tgagcggcga
     2341 agtcaggacc gatcggatcg aggagctgct gccggtcatc ttcgagcacg tggccgacgg
     2401 cgtgaccgtc cttgatcgcc gcggcgtgct gc
//
LOCUS       DATACA010000255         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_113689, whole genome shotgun sequence.
ACCESSION   DATACA010000255 DATACA010000000
VERSION     DATACA010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113689"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VHK28_05265"
     CDS             <1..275
                     /locus_tag="VHK28_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2141671.1"
                     /translation="VSNDTGASHLAAAVGAPSVIVFPASDPARWAPPDRVLHRALGGP
                     VNLPESDHCCLGESCAELAIQWQDVQVADVLQAVQEQLERGVRHAA"
     gene            265..1269
                     /locus_tag="VHK28_05270"
     CDS             265..1269
                     /locus_tag="VHK28_05270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2141672.1"
                     /translation="MPPDTLNVLTWHVHGSYLESLAACGHRIYLPVGRGDEPGYAGRT
                     DGFDWPSNLVEVDAARVSDLELDVVLSQSVRNWQQDRHEILSPEQLREVPMVHVEHDP
                     PREHPTDTRHPIDDPDALIVHVTHFNRLMWDSGRTPTAVIEHGVRVPDDVTYTGELER
                     GLVVVNGLGWRGRRLGLDLFLAARERLPLDLVGMESIELGGLGEVRPRELPRLMGRYR
                     FFFHPVRYTSLGLALLQAMAVGVPPVALATTEVVETIRDRVSGFTSTNPDVLHRRMEQ
                     LLEDAELAHRLGAGARHTARTRYGIDRFAAEWHALLADVTQRRRRGARRDPQVMEEVA
                     "
     gene            1266..>2587
                     /locus_tag="VHK28_05275"
     CDS             1266..>2587
                     /locus_tag="VHK28_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX2141673.1"
                     /translation="MKRRVALISEHASPLAMLGGVDCGGQNIYVGQVARHLVSLGYEV
                     DVLTRRDAPDQPSEVRWANGVRVLHIDAGPLAPIAKEDLLPHMPAFLREATSLARDRS
                     WDLVHANFFMSGWVAAELKRRLGIPFVVTFHALGRIRRLHQGDADRFPAERLDIEERL
                     VREADHLVAECPQDEEDLVRHYDADPRRITIVPCGFDRTEFWPISRPLARVVLGLEAD
                     EPVLLQLGRMVPRKGVETVIESLVHLRERGHRPRLLIVGGESERPDPNLTPEIGRLQG
                     VADELGVSDQVTWVGRRGRDLLKYYYSAADLFLTVPWYEPFGITPVEAMACGTPVVGS
                     NVGGIKFSVRDGETGYLVPPRDARALGERIGHLLDHPRVASVFRRQATRRVNDLFTWE
                     RVVSSLSDLYEDVIDASQPMPACSRRERQLVDASIEGAVEALRETHRQS"
BASE COUNT          369 a          901 c          920 g          397 t
ORIGIN      
        1 tggtcagcaa cgacaccggc gcctcgcacc tggcggccgc cgtcggcgcg ccgagcgtca
       61 tcgtctttcc cgcctccgat cccgcgcgct gggctccgcc agatcgcgtg ctgcaccggg
      121 ccctcggcgg cccggtcaac ctgccggagt cggaccactg ctgcctgggc gagtcctgcg
      181 cggagctcgc gattcagtgg caggacgtcc aggtcgcgga cgtcctgcag gcggtgcagg
      241 agcagctcga gcggggtgta cgacatgccg cctgacacgc tgaacgtgct gacctggcac
      301 gtgcacggca gctacctcga gtcactcgcc gcctgcgggc accgcatcta cctgcctgtc
      361 ggccgcggcg acgagcccgg ctacgccggc cggacagatg ggttcgactg gccatccaac
      421 ctggtggagg tcgacgcggc ccgcgtatcc gacctggagc tggacgtcgt cctctcgcag
      481 agcgtgcgca actggcagca ggaccggcac gagatcctgt cgccggagca gctgcgcgag
      541 gtgccgatgg tgcacgtcga gcacgacccg ccgcgcgagc atccgaccga cacccgtcat
      601 ccgatcgatg accctgatgc gctgatcgtg cacgtcacgc acttcaaccg cctcatgtgg
      661 gacagcggcc gtacgccgac ggcggtcatc gaacacggcg tccgagtgcc ggacgatgtc
      721 acctacaccg gcgagctcga acgcgggctg gtggtcgtca acgggcttgg ctggcgcgga
      781 cgccggctgg ggctcgacct gttcctggcg gctcgcgagc gcctgccgct ggacctggtg
      841 gggatggagt cgatcgagct cggcgggctg ggcgaggtca ggccgcggga gctgccgagg
      901 ctgatggggc gctatcggtt cttcttccac ccagtgcgct acaccagcct gggcctggca
      961 ctgctgcagg ccatggccgt cggtgttccg ccggtggcat tggcgaccac ggaggtcgtg
     1021 gagaccatcc gcgaccgcgt cagcggcttc acctcgacca acccggacgt ccttcaccgg
     1081 cgcatggagc agctcttgga ggatgccgag ctcgcccacc gactgggcgc cggcgcgcgg
     1141 cacaccgcac ggacccggta cgggatcgat cgtttcgccg ccgagtggca tgcgctgctc
     1201 gcggacgtga cccaacgtcg tcggcgcggg gcgcgccggg acccgcaggt tatggaggaa
     1261 gtggcatgaa gcggcgcgta gccctcatca gtgagcacgc ctcgccgctg gccatgctcg
     1321 gtggcgtgga ctgcggcggg cagaacatct acgtgggcca ggttgcccgc catctcgtca
     1381 gcctcgggta cgaggtggac gtgctcaccc gccgggacgc accggaccag ccatcagagg
     1441 tgcgctgggc gaatggtgtg cgcgtcctgc acatcgatgc cggcccattg gcgccgattg
     1501 ccaaggagga tctgctgccc cacatgcccg ccttcctgcg ggaggccacc tcattggccc
     1561 gggaccgctc ctgggacctg gtgcacgcca acttcttcat gtccggctgg gtggccgccg
     1621 agctgaagcg acggctcggc atcccgttcg tggtcacctt ccacgcgctg gggcgcatcc
     1681 gccggctgca ccagggcgac gcggaccgat tcccggctga gcggctcgac atcgaggagc
     1741 gcctggtgcg tgaggcggac cacctggtgg ccgagtgccc gcaggacgag gaggacctgg
     1801 tccgccacta cgatgccgac ccgcgtcgca tcaccatcgt gccctgcggg ttcgaccgca
     1861 ccgagttctg gcccatcagc cgcccactgg cgcgggtggt actcggcctg gaggcggatg
     1921 agccggtcct cctccagctg ggtcggatgg tgccgcgcaa gggcgtggag accgtcatcg
     1981 agtcgctggt ccaccttcgc gagcgcggtc accggcccag gctgctgatc gtcggcgggg
     2041 aatccgagcg accggatccc aacctcaccc cggagatcgg gcggctgcag ggcgtggccg
     2101 acgagctggg cgtctccgac caggtcacct gggtcgggcg ccgcgggcgc gacctgctga
     2161 agtactacta ctcggccgcc gacctgttcc tgaccgtgcc gtggtacgag ccgttcggca
     2221 tcaccccggt ggaggccatg gcctgcggga cgccggtggt tggctccaac gtcggcggca
     2281 tcaagttcag cgtgcgggac ggtgagaccg gctacctcgt cccaccccgc gatgcccggg
     2341 cgctcggtga gcggatcggg catctcctgg atcacccgcg cgtggcatcg gtcttccgcc
     2401 gccaggcgac ccgcagggtg aacgacctgt tcacctggga gcgcgtggtg tccagcctca
     2461 gcgatctgta cgaggacgtg atcgacgcca gccagccgat gccggcctgc agccgccgcg
     2521 agcggcagtt ggtggacgcc agcatcgagg gtgcggtgga ggccctgcgc gagacgcacc
     2581 ggcagtc
//
LOCUS       DATACA010000256         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_917644, whole genome shotgun sequence.
ACCESSION   DATACA010000256 DATACA010000000
VERSION     DATACA010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917644"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..1230)
                     /gene="recO"
                     /locus_tag="VHK28_05280"
     CDS             complement(424..1230)
                     /gene="recO"
                     /locus_tag="VHK28_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX2141674.1"
                     /translation="MSPRARLYKTEAIVLRSMDLGEADRVLTVLTPHLGKLRIIAKGV
                     RRTRSRLGGGLEPFSDVHLVLAVGRTFDVVTQAALEDAHLGLRDDLHSTAAAWYLVEL
                     ADRFCEGAADSRGAFLLLAQGLAALDAAPADVRREVVARWFELALLDVMGFRPELVRC
                     LECGADIGPEGNAYSAVAGGVLGPECAHDATGARPVSPDALKVMRHLQRTPLVDVLRV
                     RLPASVHREVERLLHATVSAVLERELRSRDFLEEVAAREAAPGPAAPVPH"
     gene            1249..2280
                     /locus_tag="VHK28_05285"
     CDS             1249..2280
                     /locus_tag="VHK28_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2141675.1"
                     /translation="MDIRTEFTDLLGDIENEMRDVLAEQDEHARPLYEMLSYHLGLDQ
                     PDGPRGKRMRPLLGLLAYQSITGDYHRALPGAAAVELGHNFSLVHDDIEDADRERRHR
                     PTLWAIWGVPLAINAGDALFALSRLALYRLLEDGFSEGRVLALMRVYDETCLALCEGQ
                     FLDISFERRTDVTVDEYMEMIGRKTAALVGASVQAGAILATDDADTIEAYRRFGWHLG
                     LAFQMADDVKGTFWPSEASGKPEAGDVRKRKKTLPLVWALEHATDADRARLLEIYGPA
                     AAPSDGPMGPDLVDEVLAILERAGAREHALAEARRHRDLALEDAGSLPVGERQRAELR
                     ALVESVIAL"
     gene            2313..2777
                     /locus_tag="VHK28_05290"
     CDS             2313..2777
                     /locus_tag="VHK28_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX2141676.1"
                     /translation="MRPLLQWLGLAFAPAAIGLPFTIRAPQYYRRLDKPGWSPPSGAF
                     GPVWSVLYVLMGVAAWLVSQSPSRGDGRRTALRLFGVQLVLNAAWTPIFFGLRRPGLA
                     LAEIVATLAAIAATTTAFFRQRPLAGVLLLPYLAWVGFATALNAAIWRRNRD"
     gene            complement(2784..4157)
                     /locus_tag="VHK28_05295"
     CDS             complement(2784..4157)
                     /locus_tag="VHK28_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX2141677.1"
                     /translation="MNDFPFLDLAAVLGLILIGGFFAASEIALITVKRHRLNQLVGEG
                     SRAARIAQRLTDDPSRFLATIQIAITFLGFMASAVGALALSDSLAGLVALIPFVEEEI
                     ARQVSFVLVTLMIALASIIIGELVPKTLALTFADRFALIVARPIGWLDFVLRPVVWFV
                     ASVSNVLVRMLSGRERPQAGYLSTEELKMLVETGSETGGIEEDEKEMIHGVIELAEKQ
                     VHEVMVPRIGIRAVEVSDSTDQVLDVIVRGGHSRVPVFDENLDNIVGILYAKDLLPYL
                     KRNGEPAREIEIGKLARPAVYVPESKRVDDLLHEMQAAKRHIAIVVDEYGGTAGLITM
                     EDMVEEIVGEIQDEYDVEEPMVELLERDGQTAYRVDGRVSMDDLRDLFDLPDDEEEDE
                     DAYDTVGGFIVHRAGSIPLPGATVPFRDDVTLSVEVSEPRRVAKVIASRPNPDEQELG
                     VANDDGE"
     gene            complement(4212..4733)
                     /locus_tag="VHK28_05300"
     CDS             complement(4212..4733)
                     /locus_tag="VHK28_05300"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2141678.1"
                     /translation="MEIGPYSHLRPGCRIGPRCRIGNFAELKQTRMGSGTQQHHFSYL
                     GDAQVGEDVNIGAGAVTANYDGAQKHPTMIGDGAFIGVDTMLRAPITIGPGAKTGAGA
                     VVTRDVAPGKTVVGMPARPIEARRRSRPELLPPDSGGEPAVAAEPAEPAEPAAAAAER
                     NPHAGGTPNPSDA"
     gene            complement(4652..>5178)
                     /locus_tag="VHK28_05305"
     CDS             complement(4652..>5178)
                     /locus_tag="VHK28_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141679.1"
                     /translation="CFDAAWLRANVGNVPASASGEYYLTDLVALATAGGRRVAVVSAP
                     RPELTTGINDRVALAEAERLLRAEIAERHMRNGVTIVDPANTFIDAAVEIGQDVRIEP
                     WTVLSGTTAIAQDAVIGPNATVRDSRIGRGPACGPASSSPRRSPRTWRSGRTATFDPA
                     AASVHAAASATSRS"
BASE COUNT          739 a         1839 c         1791 g          809 t
ORIGIN      
        1 cgccgcttgg cgagcgagac gacgacctcc atgttcggag caggcacggc tgtcgaaggc
       61 tcctccggtg cgggctgcag atggaccgat gggcggtctc ggccgacttc cgagtctagc
      121 gcagacaccg gcatgcgctg cggcgagtga ccatgccggc tgccgtcctc acgtgcgaat
      181 ggtgatcgcg ttatcggcgg tgcgggtcta ttccaccctc aggtgcgaat ggtgatcgca
      241 ctagagaggt tctggggcgc ttccgttctc agatgtcgct cggcattcgc gtcggagagc
      301 gtccgtgggc cgttccacgc tcatcggctg ccgcaggcga ccggcgcgtg gtctctaacg
      361 cgaatgacgt tcgcatatga gagtgcgccg attcccgaca ctgcacggcg cgcggccggg
      421 gcgtcagtgg gggacgggcg ccgccggtcc tggtgccgcc tctctggcgg ccacttcctc
      481 caggaagtcg cggctgcgca gctcccgctc cagcacggcc gacaccgtgg cgtgcagcag
      541 ccgctccacc tcgcggtgga ccgatgcagg aagccgcaca cgcagcacgt ccaccagcgg
      601 cgtgcgctgc aggtggcgca tgaccttgag cgcgtcgggc gagaccggcc gcgcgccggt
      661 ggcatcgtgc gcgcactccg ggcccagcac gccgccggcc actgccgagt aggcgttccc
      721 ctccggaccg atgtccgcac cgcactccag gcagcgcacc agctcgggcc ggaagcccat
      781 gacgtccagc agggcgagct cgaaccagcg ggcgaccacc tcgcggcgga cgtcggccgg
      841 ggcggcgtcc agcgccgcga ggccctgggc cagcagcagg aatgcgcccc gcgagtcagc
      901 agctccctcg cagaagcggt cggccaactc gaccaggtac caggcggcag ccgtggagtg
      961 caggtcgtcg cgcagtccga ggtgtgcgtc ctcgagcgcc gcctgggtga cgacgtcgaa
     1021 ggtgcggccc acggccagca ccaggtgcac gtcgctgaac ggctccagcc cgccgcccag
     1081 ccgcgagcgc gtgcgtcgca cgcccttggc gatgatgcgc agcttgccga ggtgcggcgt
     1141 caggacggtg agcacccgat cggcctcgcc gagatccatg ctgcgcagca cgatcgcctc
     1201 ggtcttgtac aggcgggcgc gcgggctcac ctcgggtacc cttcgggcgt ggacatcagg
     1261 accgagttta ccgacctgct cggcgacatc gagaacgaga tgcgggacgt cctggccgag
     1321 caggacgagc acgcgcggcc gctgtacgag atgctctcct accacctggg cctggaccag
     1381 ccggacgggc cacgcggcaa gcgcatgcgg ccgctgctcg gcctcctcgc ctaccaatcc
     1441 atcaccggcg attaccaccg agccctgcca ggcgccgccg cggtggagct ggggcacaac
     1501 ttcagcctgg tgcacgacga catcgaggac gcggaccgcg agcggcgcca tcggcccacc
     1561 ctgtgggcga tctggggcgt cccgcttgcc atcaacgccg gcgacgccct gttcgcgctg
     1621 tcgcgcctgg ccctctatcg gttgttggag gacggtttca gcgaggggcg cgtgctggcc
     1681 ctgatgcgcg tgtacgacga gacctgcctg gcactctgcg agggccagtt cctggacatc
     1741 agcttcgagc gccgcacgga cgtcacggtg gacgagtaca tggagatgat cgggcgcaag
     1801 acggcggcgc tggtcggtgc atcggtccag gccggggcca tcctggccac cgatgacgcc
     1861 gacaccatcg aggcctaccg ccgcttcggc tggcacctgg gtctggcctt ccagatggcc
     1921 gatgacgtga agggcacctt ctggcccagc gaggcctctg gcaagccgga ggccggggac
     1981 gtccgcaagc ggaagaagac cctgcccctg gtctgggcgc tggagcacgc gaccgatgcc
     2041 gaccgggcgc ggctgctgga gatctacgga cctgccgcgg cgccctcgga tggacccatg
     2101 ggcccggacc tggtggacga agtgctcgcc attttggagc gggccggtgc ccgcgagcac
     2161 gccctggccg aggcacgccg ccaccgggac ctggcgctgg aggatgccgg cagcctgccg
     2221 gtcggcgagc ggcagcgggc cgagctccga gccctggtcg agtcagtgat cgccctctag
     2281 ctgttggcac acgccatgcc tcgcgggcgg gcatgcgtcc actcctccaa tggctcgggc
     2341 tcgccttcgc gccggcggcc atcgggctgc cgttcaccat ccgcgcaccg cagtactacc
     2401 gccggctgga caagccgggg tggtcgccgc cgtcaggggc cttcggcccg gtgtggagcg
     2461 tcctctacgt cctgatgggt gtcgcggcct ggctggtgtc gcaatcgccg tcgcgcggag
     2521 atggtcgccg taccgcgttg cgcctcttcg gcgtccagct ggtgctgaat gcggcatgga
     2581 cgccgatctt cttcggcctg cgccggcccg gcctggcgct ggccgagatc gtggccaccc
     2641 tggccgcgat cgccgccacc accacagcgt tcttccgcca gcggccgctg gccggcgtcc
     2701 tgctcctccc ctacctcgcc tgggtggggt ttgccacggc gctcaacgcc gccatctggc
     2761 gccgtaaccg ggactagcgc gccttactca ccgtcatcgt tggcgacgcc gagctcctgc
     2821 tcgtccgggt tcggacgcga ggcaatcacc ttggccaccc tacgtggctc ggagacttcc
     2881 acgctgaggg tcacgtcgtc gcggaacggc acggtcgcgc cgggaagggg gatgctgccg
     2941 gcgcggtgca cgatgaagcc gccgaccgtg tcgtacgcgt cctcgtcctc ctcctcgtca
     3001 tcgggcaggt cgaacaggtc gcgcaggtcg tccatgctga cccggccgtc gacccggtag
     3061 gcggtctgcc cgtcgcgctc gaggagctcg accatgggct cctcgacgtc gtactcgtcc
     3121 tggatctcgc cgacgatctc ctcgaccatg tcctccatgg tgatcaggcc cgcggtgccg
     3181 ccgtactcgt ccaccacgat ggcgatgtgc cgcttggcgg cctgcatctc gtgaagcaga
     3241 tcgtccacgc gctttgactc cgggacgtag acggccggcc gcgccagctt cccgatctcg
     3301 atctcccgcg ccggctcacc gttgcgcttc aggtacggca gcaggtcctt agcgtacagg
     3361 atgcccacga tgttgtccag gttctcgtcg aagaccggca cccgcgagtg gccgccgcgc
     3421 acgatgacgt ccagcacctg gtcggtgcta tcgctgacct cgacggcgcg gatcccgatg
     3481 cgcggaacca tcacctcgtg gacctgcttc tcggcgagct cgatgacccc gtggatcatc
     3541 tccttctcgt cctcctcgat gccgccggtc tccgagccgg tctcgaccag catcttgagc
     3601 tcctcggtgg acaggtagcc ggcctgcggc cgctcgcggc cgctgagcat gcggacaagc
     3661 acgttggaga ccgatgccac gaaccacacg accgggcgca gcacgaagtc cagccagccg
     3721 atgggccggg ccacgatcag cgcgaagcgg tcggcgaagg tgagcgccag ggtcttgggc
     3781 accagctcgc cgatgatgat cgaggccagc gcgatcatca gggtgaccag cacgaagctc
     3841 acctgccgcg caatctcctc ttccacgaac gggatcagcg ccaccagccc ggcgagcgag
     3901 tcggagagcg ccagcgcacc gacggccgac gccatgaaac ccaaaaaagt gatggcgatc
     3961 tggatggtgg ccagaaagcg gctgggatca tcggtcaggc gctgggctat gcgcgccgcg
     4021 cggctgccct cgccgaccag ctggttgagc cggtggcgct ttacggtgat gagcgcgatc
     4081 tcggaagcgg cgaagaagcc accgatcagg atcagtccca gcaccgcggc gaggtcgagg
     4141 aacgggaagt cgttcatctc accgcgctcg gtgccgggcg ccgttgaccg gcggcgccgc
     4201 tactgctgcc ttcaggcatc ggaggggttc ggggtacctc ctgcgtgcgg gttccgttcc
     4261 gccgcggcag cggcgggctc cgccggttcg gcgggttccg cggcgacggc gggttcgcca
     4321 ccggaatccg gtggcaggag ctcgggccgg ctgcgacgac gcgcctcgat cgggcgtgcc
     4381 ggcatgccga ccaccgtctt gccgggcgcc acgtcacgag tgaccaccgc cccggcgccg
     4441 gtcttggcgc ccggaccgat ggtgatcggc gcccgcagca tggtgtccac gccgatgaag
     4501 gcaccgtcgc cgatcatggt ggggtgcttc tgggccccgt catagttggc ggtcacagcg
     4561 ccggcaccga tgttcacgtc ctcgcccacc tgcgcatcgc ccaggtagct gaagtggtgc
     4621 tgctgcgtgc cggaccccat cctcgtctgc ttcagctccg cgaagttgcc gatgcggcag
     4681 cgtggaccga tgcggcagcc gggtcgaagg tggctgtacg gcccgatctc cacgtcctcg
     4741 gcgacctccg aggactcgat gacgctggcc cacacgctgg tccgcgcccg atccgggagt
     4801 cgcgcaccgt ggcgttcggc ccgatcaccg cgtcctgagc gatcgcggtg gtcccgctca
     4861 gcaccgtcca cggctcgatc cgcacgtcct ggccgatctc caccgccgca tcgatgaagg
     4921 tgttggccgg gtccacgatg gtcacgccgt tgcgcatgtg gcgttccgcg atctcggcac
     4981 gcagcagccg ctcggcctcg gccagcgcca cgcggtcgtt gatgccggtg gtgagctccg
     5041 gtcgcggggc gctcaccacc gctacgcgtc gaccgccggc cgtcgccagc gccaccaggt
     5101 cggtgaggta gtactcgccc gaggcggacg cgggcacgtt gccgacgttg gcccgtagcc
     5161 acgccgcgtc gaagcagt
//
LOCUS       DATACA010000257         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1077833, whole genome shotgun sequence.
ACCESSION   DATACA010000257 DATACA010000000
VERSION     DATACA010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077833"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1217
                     /gene="hemE"
                     /locus_tag="VHK28_05310"
     CDS             165..1217
                     /gene="hemE"
                     /locus_tag="VHK28_05310"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX2141680.1"
                     /translation="MLDLPTRAMTGEERFLAALHHRQPDATPVWFMRQAGRALPAYRA
                     LRERHDILTLAKTPELCAEVTIMPVGEFGVDAAVMFADIMLPLEPMGIELEIEPSIGP
                     IIHNPVRSMADVERLRAIEPSHDISFLMDAIRTVRGALAGRQAVIGFSGAPFTLACYL
                     IEGRPSRDYQLAKAFMYREPAAWHALMDRLTDMVIGYLNAQVEAGAQAVQLFDSWVGA
                     LSPGDYGTYVQAYVARIFSAVHGAPTIHFGTGTAALLELMADAGGDVIGVDARQSLDD
                     AWVRIGHERGIQGNLDATRLLAGWDATRFGALDVLDRAKNRPGHIFNLGHGVLPETDP
                     DLLRRLVLLVHEESAR"
     gene            1214..2167
                     /gene="hemH"
                     /locus_tag="VHK28_05315"
     CDS             1214..2167
                     /gene="hemH"
                     /locus_tag="VHK28_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX2141681.1"
                     /translation="MSERGQDPLGVLLMTYGSPDGPDDMPRYLAAVRGGRDASDELVI
                     EFRRRYEAIGGSPLIPITQAQAAAVEDHLRNEGVNARVSAAMRFSEPSISRELAKLVE
                     AGARSVVGVVMSPQQSSMLMGGYATALRNAADAAEPTPWVTVAPAWYREAGFLDAVAD
                     RIRAGLDSLGPHQRETAPVLLTAHSLPRRVAEAEPQYLDQLRETAALVARRAALAPER
                     WSFCWQSAGHEPGEWMKPDFADLLPELQRAGHRSVLVAPIQFLADHLEVLYDLDIGAR
                     EQAEAHGISFTRIESLNVHPSFIAALAAVVTHASAEVRALA"
     gene            2199..>2387
                     /locus_tag="VHK28_05320"
     CDS             2199..>2387
                     /locus_tag="VHK28_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2141682.1"
                     /translation="MVPLLTNRALNRALLERQLLLRRVRMPVEEAIEHLVAVQAQEPR
                     DPYIGLWCRLDGFQTDDLA"
BASE COUNT          364 a          807 c          823 g          393 t
ORIGIN      
        1 cgaggcggca ttcgcacgct tcttccacgc catgctggat gcaggcgtca tgctgccgcc
       61 gtcccagcag gaagcatggt tcatctcaac ggcgcatcag ccgtccgatc tcgatttcac
      121 cctcgcggcc gcggaggccg cgttcgcgac tgcaggagaa ccccatgctt gacctcccaa
      181 cccgagccat gaccggagag gagcgctttt tggccgcgct gcaccatcgg cagccggatg
      241 ccacgccggt gtggttcatg cggcaggcgg gtcgcgcatt gccggcgtac cgtgcgctcc
      301 gggagcgtca cgacatcctg accctggcca agacgccgga gctgtgcgcc gaggtcacga
      361 tcatgccggt cggggagttc ggcgtggatg ccgccgtgat gttcgccgac atcatgcttc
      421 cgctcgagcc catggggatc gagctggaga tcgagccttc gatcggtccc atcatccaca
      481 acccggtgcg ctccatggcc gacgtcgaac gcctgcgggc catcgagcca agccacgaca
      541 tttcctttct catggacgcc atccggaccg tccgaggcgc gctcgccggg cgccaggcgg
      601 tgatcggatt ctcgggcgcc cctttcaccc tggcctgcta cctgatcgag ggacgcccct
      661 cgcgtgacta ccagctcgcc aaggctttca tgtaccgtga gccggcagcc tggcatgcgc
      721 tgatggaccg gctcacggac atggtcatcg gttacctgaa cgcccaggtc gaagcgggcg
      781 cccaggctgt gcagctcttc gactcgtggg tcggagccct ctcgccgggc gactatggca
      841 cctacgtcca ggcgtacgtg gcccgcatct tctccgcggt ccacggagca cccaccattc
      901 acttcgggac cgggacagcg gcgctcctcg agctgatggc agatgccggc ggcgatgtga
      961 tcggcgtaga tgcccgccag tcgctggacg acgcgtgggt gcgcatcggt catgagcgtg
     1021 gaatccaggg gaacctggac gcgacccggc tcctggccgg ctgggatgcc acgcgatttg
     1081 gtgcccttga tgtgctggat cgggcgaaga atcgaccggg ccacatcttc aacctgggtc
     1141 acggcgtgct gccggagacg gaccccgacc tcctgcgccg cctggtcctt ctggtgcacg
     1201 aggagtcagc gaggtgagcg agcgcgggca ggatccgctc ggcgtgctgc tgatgaccta
     1261 cggatcgccg gacggtccgg acgacatgcc ccggtacctc gccgcggtac gcggtggacg
     1321 cgacgcctcg gacgaactgg tcattgagtt ccggcgccgg tacgaagcga tcggcggttc
     1381 accgctgatc ccgatcacgc aggcccaggc ggcggcggtc gaggaccacc tgcgaaacga
     1441 gggtgtgaat gcgcgcgtgt cggcagcgat gcgcttctcg gaaccgagca tctcgcgaga
     1501 gctcgcgaag ctcgtggagg ctggtgcgcg gtcggtcgta ggagtggtga tgagcccgca
     1561 gcagtcctcg atgttaatgg ggggatatgc cacagcgctg cgtaacgccg cggacgcggc
     1621 tgaaccaacg ccgtgggtca cggtggcgcc cgcctggtac cgagaggccg gcttcctgga
     1681 tgcggtcgcg gaccggattc gcgcgggact ggacagcctg ggcccccatc agcgggagac
     1741 agcgccggtg ctgcttaccg cgcactccct gccgcggcgg gtggccgagg cggagccgca
     1801 gtacctcgac cagctgcgcg agaccgcggc gctggttgca cgccgtgcgg ccctggcgcc
     1861 agagcgctgg tcgttctgct ggcagtcagc cgggcacgag cccggcgagt ggatgaaacc
     1921 ggacttcgcc gacctgctgc cggagctgca gcgtgcgggc caccggtccg tgctggtcgc
     1981 gcccatccag ttcctggccg atcacctgga ggtgctgtat gacctggaca tcggcgcccg
     2041 cgagcaggct gaagcacacg ggatcagctt cacccgcatc gagtcgctga acgtgcaccc
     2101 cagcttcatc gctgccctgg cagcggtcgt gacgcacgcc tcggcggagg tcagggcgct
     2161 ggcctgacgc catcgcggta cccgtaccat gcggcggcgt ggtaccgctg ctgaccaacc
     2221 gtgccctgaa ccgggcgctc ctggagcgcc agctcctgct gcgacgagtt cggatgcccg
     2281 tcgaggaggc gatcgagcac ctcgtggcgg ttcaggccca ggagccccgc gacccgtaca
     2341 tcggcctgtg gtgccggctg gacggtttcc agaccgatga cctggcc
//
LOCUS       DATACA010000258         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2763866, whole genome shotgun sequence.
ACCESSION   DATACA010000258 DATACA010000000
VERSION     DATACA010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763866"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VHK28_05325"
     CDS             <1..171
                     /locus_tag="VHK28_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX2141683.1"
                     /translation="TIIVRQRGSTFTPGPNVGMGRDYTLFATADGTVRFSHLTRDKKQ
                     VSVQANAAEVPA"
     gene            168..380
                     /gene="rpmE"
                     /locus_tag="VHK28_05330"
     CDS             168..380
                     /gene="rpmE"
                     /locus_tag="VHK28_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX2141684.1"
                     /translation="MKQGVHPKYHQAEVHCIGCGTTFTVGSTVESIRVEVCSNCHPFY
                     TGTQNIVDTAGQVERFQRRMERAAKS"
     gene            410..1498
                     /locus_tag="VHK28_05335"
     CDS             410..1498
                     /locus_tag="VHK28_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX2141685.1"
                     /translation="MAEPMRSRPTGWRALLAPHLVFLIPELIMAAGPFVITAVARAGE
                     KPRFRMPDFFYGGQALIEGVMMRGRTTVAMSVRPPSGEIRTFSEPLPAALREGRWLKV
                     PFVRGMFVLYETLVLGTRMLMRSAAIAAEGEDIEISRGMMVGTMIFSIGFAVALFFVL
                     PLFLSTFAEDATESDLLANLAEGGIRLVVFVAYIGLIGMMGDIKRVFAYHGAEHKAIS
                     ANEARRPLTPEEVDRFSTAHTRCGTTFLLIVVVISILFFSLIPRTGVPLPVLFLSRIA
                     LVPFIAAVAYELVRFGARYYGNALVRAIYAPGLWLQSLTTRKPDRSMLEVSIASLESC
                     LAADREAGRPAVAEPTAGVVDPPTGVVP"
     gene            1780..>2829
                     /gene="prfA"
                     /locus_tag="VHK28_05340"
     CDS             1780..>2829
                     /gene="prfA"
                     /locus_tag="VHK28_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX2141686.1"
                     /translation="MIDRLREIEARFDEVAAAMSSPDAAADPRRLTELGRELSRLEPV
                     VNAIRSWAGVRDQLAEAREMARDPDDEVRAMAREEVAALEERERMLEQELRTHLVPRD
                     PNDDRDVILEVRAGAGGDEAALFAADLFRMYARYAERNRWKLELLSSSEGAAGGFKEV
                     IAEVRGPGAYSRLKFESGVHRVQRVPVTEAQGRIHTSTATVSVLPEAEEVEIDIPEGD
                     LRVDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVAIQDEKSQHKNKAKAMAVLRARLL
                     EMEQQRQAEERGEQRRSQVGSGERSEKIRTYNFPDDRVTDHRIGLTVHNLPGLLEGDL
                     DRVVDPLADADQAARL"
BASE COUNT          440 a          983 c          978 g          428 t
ORIGIN      
        1 accatcatcg tccgccagcg cggcagcacc ttcacgcccg gccccaacgt ggggatgggc
       61 cgcgactaca cgctgttcgc caccgctgac ggcacggtca ggttcagtca cctgacccgc
      121 gacaagaagc aggtcagcgt gcaggccaac gccgccgagg tgccggcgtg aagcagggcg
      181 tccatccgaa gtaccatcag gcggaggtgc actgcatcgg ctgtggcacc accttcacgg
      241 tcggctcgac cgtggagtcg attcgcgtcg aggtgtgctc gaattgccac ccgttctaca
      301 ccggcaccca gaacatcgtg gacaccgccg gccaggtcga gcggttccag cgtcgcatgg
      361 agcgggcggc caagagctga ccgggtcgac gccatcccag gctgaaccga tggctgaacc
      421 gatgcgctcg cgcccgacag ggtggcgggc gcttcttgcg cctcacctgg tcttcctgat
      481 ccccgagctg atcatggcgg ccgggccgtt cgtgatcacg gccgtggccc gcgcgggcga
      541 gaagccgcgc ttccggatgc cggacttctt ctacggcggc caggccctga tcgaaggggt
      601 catgatgcgc ggccgcacca cggtggccat gagcgtgcgg ccgccgtcgg gcgagatccg
      661 caccttttcc gagccgctgc ccgctgccct gcgcgagggt cgctggctga aggtgccctt
      721 cgtgcgcggc atgttcgtgc tgtacgagac gctggtgctc ggaacccgga tgctgatgcg
      781 ctcggccgcc atcgccgccg agggcgagga catcgagatc agccgcggaa tgatggtggg
      841 gaccatgatc ttctccatcg gcttcgcggt ggccctgttc ttcgtcctgc cgctgttcct
      901 ctcgaccttc gccgaggacg ccaccgagtc ggacctgctg gccaacctgg ccgagggcgg
      961 catccggctg gtggtgttcg tggcctacat cggcctgatc ggcatgatgg gcgacatcaa
     1021 gcgcgtcttc gcctaccacg gggccgagca caaggcgatc agcgccaacg aggcgcgccg
     1081 tccgctcacc ccggaggagg tcgaccgctt ctcgaccgcc cacacccgct gcgggaccac
     1141 cttcctgctg atcgtggtcg tcatcagcat cctgttcttc agcctgatcc cgcgcaccgg
     1201 cgtgccgctg ccggtcctct tcctgtcgcg gatcgcgctg gtgccgttca tcgcggcggt
     1261 cgcctacgag ctggtgcgct tcggggcgcg ctactacggc aacgcgcttg tgcgcgccat
     1321 ctacgcccct gggctgtggc tgcagtcact gaccacccgc aagccggacc gatccatgct
     1381 cgaggtcagc atcgcctcgc tggaatcctg cttggcggcc gaccgcgagg ccgggcggcc
     1441 ggccgtggcc gagccgaccg ctggcgtggt ggacccgccg accggggtgg tgccctagcg
     1501 gctccccggc cgaatgcccg ggtcatgcag tacgcgtgca gcaggatctc agggatgtgc
     1561 gggatgaccg agaggatcgt cgcgaaatgc ggtacccgta gtgcctaatg gcgacgaccc
     1621 ggtgcctgcg gctgccgcca caggggggaa ccgccaacgt cctcgctgtt gcgccacacg
     1681 ggtacgacat ttccggatga agtcgtccaa cccgagcgac ttcgtcgcat tcctgcctca
     1741 ccgccccgac actcgccggc cgcgaccgat aatgagggca tgatcgaccg tctgcgagag
     1801 atcgaggctc gcttcgacga ggtggccgcc gccatgagct cgccggacgc cgccgccgac
     1861 ccgcgccgcc tcacggagct gggcagggag ctgtcccgcc tggagccggt ggtgaacgcc
     1921 atccgctcct gggccggcgt gcgcgaccag ctggcggagg cccgcgagat ggcccgcgac
     1981 ccggacgacg aggtccgcgc catggcccgc gaggaggtcg ccgccctgga ggagcgcgag
     2041 cgcatgctgg agcaggagct gcgcacccac ctcgttccgc gcgacccaaa cgacgaccgt
     2101 gacgtaatcc tggaggtccg tgccggagcc ggcggcgacg aggccgccct gttcgcggcc
     2161 gacctgttcc gcatgtacgc ccgctacgcg gagcgcaacc gctggaagtt ggagctcctc
     2221 tcttcgtccg agggcgcggc aggcggcttc aaggaagtca tcgccgaggt gcgcgggcct
     2281 ggcgcttact caaggctgaa attcgagtcg ggcgtgcacc gcgtccagcg cgtgccggtc
     2341 accgaggcgc agggccgcat ccacacctcc acggcgacgg tcagcgtact ccccgaggcg
     2401 gaggaggtgg agatcgacat cccggagggc gacctgcgag ttgacgtgta tcggtccagc
     2461 ggtcccggcg ggcagagcgt gaacacgacc gactcggccg tgcggatcac acacaagccg
     2521 accggcctgg tggttgccat ccaggatgaa aagagccagc acaagaacaa ggccaaggcc
     2581 atggccgtgc tgcgggcgcg gctgctggag atggagcagc agcggcaggc ggaggagcgg
     2641 ggcgagcagc gtcgctccca ggtgggcagc ggcgagcgct cggagaagat ccggacctac
     2701 aactttccgg atgaccgggt caccgaccat cgcatcggcc tgaccgtcca caacctcccc
     2761 ggcctgctgg agggcgacct ggaccgggtg gtcgacccgc tggcggacgc ggaccaggcc
     2821 gcacgcctg
//
LOCUS       DATACA010000259         1510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2443174, whole genome shotgun sequence.
ACCESSION   DATACA010000259 DATACA010000000
VERSION     DATACA010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443174"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VHK28_05345"
     CDS             <1..469
                     /locus_tag="VHK28_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141687.1"
                     /translation="DGVTAEGAADSNTTALVVQALEAAGEGDSPLVEEATAYLMTAIE
                     GTAGAVFQPGGQAQPDSSSTAFVIQALVAVGDDPSSDAWGNLLAALGTFQNDSGAFHF
                     NADDMMDNIFSTAQAIPAAAGVALPVVPTADAAIPIALLTTDGRASGAVLEAA"
     gene            621..1190
                     /locus_tag="VHK28_05350"
     CDS             621..1190
                     /locus_tag="VHK28_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141688.1"
                     /translation="MDTGEGEPIHVVVTFDDASMNAVELLRATDLDVVTVEFGGLGEA
                     VCSIVETGCDVGTCRQRVCQTGDPESPFWQYWEQDPEEGWKLSPLGASHAEIADGDIA
                     AWVWTGVEPELEPLAWDELVERAGAPAELAQGGASGEPAVYSSLVGKDEDDGTGRTGT
                     LAAVGIVAAVAVFGGWLVVRNRREQRAAE"
     gene            1247..>1510
                     /locus_tag="VHK28_05355"
     CDS             1247..>1510
                     /locus_tag="VHK28_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX2141689.1"
                     /translation="MSVPLITGRHPVLIVELLLIVLAVRTVCLPPSRRMGWGWLVRLG
                     AIAVPIGVVFNMFTVHAGDRDLFRVPSDVPIIGGTVTLNAVVYG"
BASE COUNT          224 a          477 c          534 g          275 t
ORIGIN      
        1 cgatggcgtg acggcagagg gcgccgcgga cagcaacacg accgcactgg tcgtccaggc
       61 tctcgaggcc gccggtgaag gcgactcacc actggtggag gaggcaactg cttacctcat
      121 gaccgctatc gagggcacgg ctggcgccgt gttccagccg ggcgggcagg cacagccgga
      181 ttcgagctcg accgcctttg tgatccaggc gctcgttgct gtcggtgatg acccatcgtc
      241 ggatgcctgg ggcaatctcc tggcagccct cggcacattc cagaatgaca gcggtgcctt
      301 ccacttcaac gccgacgaca tgatggacaa catcttctcc accgcgcagg caatacccgc
      361 ggcggcaggg gttgccctcc cggtcgtgcc gacggccgat gctgccatac ccatcgccct
      421 gttgacgacg gatggtcgcg ccagcggtgc ggtgctcgag gcggcctgag atgcattcct
      481 cacgcggtgt cgcacccccg cgcccatgcc tggcaacgct tgcggccatc ctggtcacac
      541 tgtggctggc cgtcgccgcg ccgtctggtg atgctcttgc tgggcagggg ggaagcgcga
      601 ccgcggcggc cgggttcatc gtggacacgg gcgagggcga gccgatccac gtcgtcgtca
      661 ccttcgatga cgcctcgatg aatgccgtgg agctgctgcg tgccacggac ctcgatgtcg
      721 taaccgtcga gtttggcggg ctgggcgagg cggtgtgctc gatcgtggag accggctgcg
      781 acgtcggcac ctgccgccag cgagtctgcc agaccggtga tcccgagtcg ccgttctggc
      841 agtactggga gcaggatcct gaggaaggct ggaagttgag tccgttaggg gctagtcacg
      901 ccgagattgc ggacggcgat atcgctgcct gggtctggac cggtgtagag cccgagctcg
      961 agcccctggc atgggacgag ctggttgagc gcgccggggc gccggcggag ctcgctcaag
     1021 ggggtgcatc cggggagccg gcggtctaca gctcgctcgt cgggaaggac gaagacgacg
     1081 gtacaggtcg aaccggcacg ctggcggcgg taggtatcgt cgcagctgtg gctgtcttcg
     1141 gcggctggct cgtcgtccgc aatcgacgcg agcagcgagc agccgagtga tgagacctga
     1201 tcgggccggt atcgactcac gcacctggct cctgtggggt gcggccgtgt cagtacccct
     1261 gatcaccggg aggcaccccg tcctgatcgt cgagctgctc ttgatcgtgc tcgcggtgcg
     1321 gacggtatgc ctgccgccat cgcgccggat gggttggggg tggctcgtgc gcctcggtgc
     1381 gatcgccgtc ccgatcggtg tggtgttcaa catgtttacc gttcacgccg gtgaccgcga
     1441 tctctttcgc gtgccgagcg atgtcccaat catcggcggc actgtcaccc tcaacgctgt
     1501 cgtctacggt
//
LOCUS       DATACA010000260         6480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1078106, whole genome shotgun sequence.
ACCESSION   DATACA010000260 DATACA010000000
VERSION     DATACA010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078106"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VHK28_05360"
     CDS             complement(<1..486)
                     /locus_tag="VHK28_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141690.1"
                     /translation="MPADSHAAHSASATAGPASPDPTPEPTPLDARVAEGLAFVQLVN
                     GVEQVFVVDADGSARQVSGHGDMATVGVVRPFWSPDRSMLALRPRSILAGTQPQLMVV
                     NADGTGQRALAQVGENGGWSPDSTRLLYEDSVLTTDTTGEPARIWLLEVATGEATVIG
                     EG"
     gene            670..972
                     /locus_tag="VHK28_05365"
     CDS             670..972
                     /locus_tag="VHK28_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family containing protein"
                     /protein_id="HEX2141691.1"
                     /translation="MATQLRDYRIVPGALDKFVEEWSTRLWPIRQGLGFILDGAWVDA
                     DQRRFVWMISYPGDWEAFAAADRAYHDSPERAALDPDPARFIEEQSTLRLAEVEVG"
     gene            complement(969..2480)
                     /locus_tag="VHK28_05370"
     CDS             complement(969..2480)
                     /locus_tag="VHK28_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX2141692.1"
                     /translation="MELFDNLSLGIDTALRLENIFWCFVGVVLGTAVGVLPGIGPTAT
                     IALLLPITFQFEPVTALIMLAGIYYGAQYGGSTTAILINLPGESSAAVTAIDGHEMAR
                     DGRAGHALAAAAIGSFVAGSFATLVVFLIAAPLARLALTFGSPELFSLVVLGLIASIA
                     LARGSAIKALAMIVLGLLLGTVGQDQFTGAPRFTFGVRELFSGIDFVSVAVGLFGVAE
                     ILKNLEEERTRELVTRTVGKLLPGRDELRRIVAPIARGTIIGTVLGILPGAGHVLASF
                     GAYSVEKRLSPRKRDFGHGTIEAVAAPESANNAAAQTSFIPLLTLGLPPHPVMALMFG
                     AFIIHGIQPGPNVITSEPALFWGLIVSMWVGNLLLLVLNLPLIGLWVRLLTIPYKFLF
                     PAIIAFAAIGTFSINFNAWDVFAIAFMGVLGYFLIKFGCEPAPLLLGFVLGPLLEDHL
                     RRSLIISRGDPSIFIDRPVSAVLLLLGVGALVLAVLPAIRRRRQEVFVEEDEP"
     gene            complement(2493..2963)
                     /locus_tag="VHK28_05375"
     CDS             complement(2493..2963)
                     /locus_tag="VHK28_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX2141693.1"
                     /translation="MPKLEIRQALKDLLAGGTFIAFGLAFAIGALGYNIGTPLRMGPG
                     YVPLVLGGVLAVLGGVIVVKGFLAGEGEPVGDVPVRAIILIVAAFLFFGITVRGLGVV
                     PALFVTIFLAALARERTSPQLALVIAVGLTVVSVVVFIMLLQLRLQLWGTWLPF"
     gene            complement(3026..4042)
                     /locus_tag="VHK28_05380"
     CDS             complement(3026..4042)
                     /locus_tag="VHK28_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX2141694.1"
                     /translation="MLIIAACTTDSGDATATAGGPQESSAATDDGDDGDGAAYFEREV
                     TFVVPFAAGGPTDTVTRLIAEPMSEELGVEIVVQNVTGAGGTVAAGEVANAQADGYTV
                     LMHHIGMSTAPTLYPDLPYDPLTDFATIGLVTEVPMTIIGSPNFEPNTMEELVTYIEE
                     NPDTVNYANAGIGAASQLCGLLFETATGLDLNEVPYDGTAPALDDLLGDQVHFMCDQT
                     TNTTEHILAEEVKAYAVTTPERIEALPDLPTTEEAGYGDIGVTVWHGLYVPAGSPQEA
                     IDALNSALLAALQDQNVIDSLADLGTGPVPQERATPEAHTEHLQSQLDLWRPIIEEAG
                     VTAG"
     gene            4262..4912
                     /locus_tag="VHK28_05385"
     CDS             4262..4912
                     /locus_tag="VHK28_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141695.1"
                     /translation="MSTDRRHPLRRLLNDAAEGRFPPADFSVEVLSSPPGRSDAVVAF
                     SAHNVIAASLQADEVHARLTDDDPATPMSAPFLAWLGRRLDASPGALDMVMVAPRSPE
                     PGQRLSLATDTAGHARVERAQRYRSDVQVYRSDDGHGLVILGRGLADRWEVSIEVEPA
                     SRGRGLGRELAVAAASLVPAGEPLFAQVSPGNVASVRAFLAAGYHPICSEVLFLRR"
     gene            complement(4923..5387)
                     /locus_tag="VHK28_05390"
     CDS             complement(4923..5387)
                     /locus_tag="VHK28_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2141696.1"
                     /translation="MIDEREEILKALRAGPLVLDRLVRELPEAALHRRPADGEWAIIE
                     VVAHLADTEERALARTRLMLAEKEPFLEPYDPAALAIERDYISMQLGEQVARFAALRA
                     EQADLLAALDDAGWLRVGRHGEHGRITVQLLAAHTAGEDADHLAQIARLIPS"
     gene            complement(5446..5521)
                     /locus_tag="VHK28_05395"
     tRNA            complement(5446..5521)
                     /locus_tag="VHK28_05395"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5486..5488),aa:His,seq:gtg)
     gene            5644..>6480
                     /locus_tag="VHK28_05400"
     CDS             5644..>6480
                     /locus_tag="VHK28_05400"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2141697.1"
                     /translation="MALYELAQAEGESLTRPVLVVAFDGWVDGGRAGTLAAEKLADDG
                     RVVATFDDDRLFDYRARRPTLEIVDGRLLDLEWPALRVMANQFHGRDVLILTGPEPDY
                     RWRALAADTVKLAQELRVATWVSLGAIPATVPHTRPVPILGTASVAGLLPEGVRQGPD
                     GQLRVPSAFVSVLEHAAARAGIPALGLFAQVPHYASGTFPTAAIELLARLGVFLGIEL
                     PLGDLPTRALETRQLLDAAVSAEERTRTYVERLEQMADEARLPEGDDLIADIERFLRE
                     GGS"
BASE COUNT         1026 a         2223 c         2253 g          978 t
ORIGIN      
        1 tccctcgccg atgacggtcg cctcgccggt tgcgacctcc agcaaccaga tgcgggccgg
       61 ctcaccggtc gtgtcggtgg tcagcaccga gtcctcgtag agcaaccgcg tcgagtcggg
      121 cgaccacccg ccgttctccc cgacctgcgc cagggcacgc tggcccgtcc cgtcggcgtt
      181 gaccaccatc agctgcggtt gagtccccgc caagattgag cgcgggcgca gcgcaagcat
      241 cgaccgatcg ggcgaccaga acggccgcac cacgccaact gtggccatgt ccccgtggcc
      301 gctcacctgc cgtgcagaac cgtcggcatc taccacgaac acctgctcca cgccgtttac
      361 gagctggacg aatgccaggc cctcggcgac gcgcgcatcg agcggcgtcg gctcaggtgt
      421 gggatccggg ctggcgggtc ccgccgtggc ggacgcgctg tgcgccgcgt gggaatcggc
      481 gggcatgggt gttccgctgg gcggcgctgc ggtgcatgcc gccagcagca ggaaggccgt
      541 gagcggcacg gcggaaagga cgcgcgcacg ccggcgcggc gagtgaatgg agctcggcat
      601 gccgtgtccc ctccgtggac gcagagcgta agccggcaga gtgtagccgc ctgtcgtacg
      661 ctccgataga tggcaacgca gcttcgcgac taccggatcg tgccgggggc gctggacaag
      721 ttcgtcgagg agtggagcac caggctctgg ccgatccgtc aggggctggg cttcatcctg
      781 gacggtgcct gggtcgacgc tgaccagcgg cgcttcgtgt ggatgatttc ctaccccggc
      841 gactgggagg cgttcgcggc ggctgaccgc gcttaccacg attcgccgga gcgcgccgcg
      901 ctggatcccg atccagcgcg tttcatcgaa gagcagtcga cgctgcggct ggccgaggtc
      961 gaggtcggtt agggctcgtc ctcctccacg aagacctcct gccggcgccg ccggatggcg
     1021 ggcagcacgg ccagcaccag ggctcccacg cccaggagca gcagcacggc cgaaacgggg
     1081 cggtcgatga agatgcttgg gtcgccgcgc gagatgatca gcgagcgccg caggtggtct
     1141 tccagcaacg ggccgagcac gaagccgagc agcagcggcg cgggctcgca gccaaacttg
     1201 atcaggaagt agccgagcac gcccatgaag gcgatggcga acacgtccca ggcgttgaag
     1261 ttaattgaga aggtgccgat ggcggcgaag gcgatgatgg ccgggaacag gaacttgtac
     1321 gggatggtca gcagccgcac ccacaggccg atcagcggca ggttcagcac aagcagcagc
     1381 aggttgccga cccacatcga gacgatcagc ccccagaaca gcgccggctc gctggtgatc
     1441 acgtttggcc cgggctgaat gccatggatg atgaatgccc cgaacatcag ggccatcacc
     1501 ggatgcggcg gcagacccag ggtgagcagt gggatgaagg aggtctgagc ggccgcgttg
     1561 ttcgccgact ccggcgcggc caccgcctcg atggtgccgt gaccgaaatc gcgcttccgc
     1621 ggcgagagcc gcttctccac cgagtaggcg ccgaagctgg cgagcacgtg accggcgccg
     1681 ggcaggatcc ccagcaccgt tccgatgatc gtgccccggg cgatcggagc cacgattcgc
     1741 cgcagctcgt cgcgtccggg cagcagcttt cccaccgtcc tcgtcaccag ctcccgggtc
     1801 cgctcctcct ccaggttctt caggatctcg gccacgccga acaggccgac tgccactgac
     1861 acgaaatcga tgccgctgaa cagctcacgg acaccgaagg tgaagcgcgg cgcgccggtg
     1921 aactggtcct ggccgacggt gcccagcagc aggcccagga cgatcattgc cagcgccttg
     1981 atcgccgatc cacgcgccag ggcaatcgac gcaatcaggc ccagcacgac cagcgagaag
     2041 agctccggcg agccgaaggt gagagcgagc ctggcgagcg gcgccgcgat caggaagacg
     2101 accagcgtgg cgaagctgcc ggcgacgaac gagccgatgg cagccgccgc cagcgcatgt
     2161 ccggcgcggc catcgcgcgc catctcgtga ccgtcgattg cggtcacggc ggccgaggat
     2221 tcgcccggaa gattgatcag gatggcggtg gtcgaaccgc catactgggc gccgtagtag
     2281 atgccggcca gcatgatcag ggcggtcacc ggctcgaact ggaaggtgat cggcagcagc
     2341 agggcgattg tcgcggttgg accgatgccc ggcagcacgc ccaccgccgt acccagtacg
     2401 acgcccacga agcaccagaa gatgttctcc agccgcagcg ccgtatcgat gccgagcgaa
     2461 aggttgtcga acagctccat gcggcgcggt cgctagaacg ggagccacgt gccccacagc
     2521 tgcaggcgca gctggaggag catgatgaaa acgacgaccg ataccaccgt cagccccacc
     2581 gcgatgacga gcgcgagctg ggggctggtg cgctcgcgcg ccagtgcggc caggaagatc
     2641 gtgacgaaca gcgccgggac gacgcccagg cctcgcacgg tgatgccgaa gaataggaac
     2701 gcggcgacga tgaggatgat cgcgcggacg ggtacgtcac cgaccggctc gccctcaccg
     2761 gccaggaagc ccttgaccac gatcaccccg cccagcacgg cgagcacgcc tcccagcacc
     2821 aacgggacgt agccgggacc catgcgcagg ggcgtgccta tgttgtagcc cagcgcgccg
     2881 atggcgaagg ccagaccgaa ggcgatgaag gtgccaccgg ccaggaggtc cttgagcgcc
     2941 tgccggatct ccagcttggg catctagcct ccaatcgaat acgatgaggc ggcctctcgg
     3001 gccgccccat cggtcaggtc aggggttagc cggccgtgac gccggcctcc tcgatgatcg
     3061 gcctccacag gtcgagctgc gactgcaggt gctcggtatg cgcctcgggc gtcgctcgct
     3121 cttgcgggac agggcccgtg ccgagatcgg ccaggctgtc gatcacgttc tggtcctgca
     3181 gcgcggccag cagcgccgag ttcagcgcat cgatcgcctc ctgcggcgag ccggccggga
     3241 catacaggcc atgccacacc gtgacgccga tgtcgccgta gccggcctcc tcggtggtcg
     3301 gcaggtccgg cagcgcctcg atgcgctccg gggtggtgac cgcgtacgcc ttcacctcct
     3361 cggccaggat gtgctcggtg gtgttggtgg tctggtcgca catgaagtgg acctggtcgc
     3421 ccagcaggtc atcgagcgcc ggcgcggtgc cgtcataggg gacctcgttg aggtcaaggc
     3481 cggtcgcggt ctcgaacagc aggccgcaca gctgcgaggc ggcaccgatg cccgcgttcg
     3541 cgtagttcac cgtgtccggg ttctcctcga tgtaggtgac cagctcttcc atcgtgttcg
     3601 gctcgaagtt ggggctgccg atgatggtca tcggcacttc ggtcaccagg ccaatggtgg
     3661 cgaagtcggt aagcgggtcg tacggcaggt cggggtacag ggtcggtgcg gtggacatgc
     3721 cgatgtggtg cataagcacc gtgtacccat ccgcctgggc gttcgccact tcgccggccg
     3781 caaccgtgcc accggcgccg gtcacgttct gcaccacgat ctccaccccg agctcctcgg
     3841 acatcggctc ggcgatgaga cgggtgacgg tatcggtcgg accgccggcc gcgaagggca
     3901 cgacgaaggt gacctcgcgc tcgaagtagg cagccccatc tccgtcgtcg ccgtcgtcgg
     3961 tggcggcgga gctttcctgc ggcccaccag ccgtggcagt ggcgtcgcca ctgtcggtgg
     4021 tgcaggcggc gatgatgagc atgagcgccg tcatcacggc cccgagccgg agcaggctcg
     4081 gtgacggggc ggcgtttgtt tccgaagcgt cgttcgctgt tgccacgtgt ctcacggacc
     4141 ggacctccag ttactgagtc acgctcggga tgcgcggacg ataccacccg cgtgtatccc
     4201 tacgggagcc gttaccaggc cggggtcatc tcgatcctgc cgggcactac tctttcgacg
     4261 catgtccacc gatcgtaggc accccctgcg ccgcctcctg aacgacgcgg ccgagggccg
     4321 ctttccgccc gccgacttca gtgtcgaggt gctgtcctca ccgccggggc gctcggacgc
     4381 ggtcgtcgcg ttttcggctc acaacgtgat cgcggccagc ctgcaggccg atgaggtcca
     4441 cgcccgcctc accgatgacg atccggcaac gccgatgagc gctccgttcc tggcctggct
     4501 cgggcggcgc ctcgatgcgt cacccggggc gctggacatg gtgatggtcg ctccccggtc
     4561 gcctgagccc ggccaacggc tgtccctggc gaccgatacg gccggtcatg cccgcgtcga
     4621 gcgcgcgcag cgctatcggt cagacgtgca ggtctaccgc tccgacgatg gccacggact
     4681 cgtcatcctc gggcgtggcc tggctgatcg ctgggaagtc agcatcgagg tcgaaccggc
     4741 gtcgcggggc cgcggcctgg gacgggagct ggcagtcgcc gccgcatcgc tcgttccagc
     4801 cggtgagccg ctgttcgccc aggtgtcacc cggcaacgtg gcatccgtgc gcgccttcct
     4861 ggcggcgggc taccacccca tctgctccga ggtgctcttc ctgcggcggt gagcctggcg
     4921 gctcacgacg ggatgagtcg cgcgatctgc gcgaggtgat cggcgtcctc acctgcggtg
     4981 tgcgcggcca gcagctgaac cgtgatgcgc ccgtgctcgc cgtggcgacc cacccgcagc
     5041 cagccggcgt catccaatgc ggccaggagg tcggcttgct cggctcgaag cgccgcgaag
     5101 cgcgccacct gctcgcccag ctgcatcgag atgtagtcac gctcgatggc cagcgccgcg
     5161 gggtcgtacg gctcgaggaa tggttccttc tcggccagca tcaaccgcgt ccgggccagg
     5221 gcgcgctcct ccgtgtcggc gaggtgagcg accacctcga tgatcgccca ctcgccgtca
     5281 gcgggcctgc ggtgcagcgc ggcctcgggc agctcgcgga ccagccggtc taggacgagt
     5341 ggaccggccc gcagcgcctt caggatctcc tcgcgctcat cgatcatctg ccacctccac
     5401 ggcgctggct cgacatcggg caggacagcg taccggcgac gaaactgggg tggcctacgg
     5461 gggtcgaacc cgtaaccttc ggatccacaa tccgatgctc tgccggttga gctaaggcca
     5521 ccacgggtag ccgcgcgtgg tcgcagcgcg gccgccgaat tctcgcattc gcccgagtgc
     5581 cggtcaaacg accgctaggt cccccattcc ggaccgatgc ggcccttccc gtatgcttgc
     5641 ggcatggcgc tgtacgagct ggcccaggca gagggcgaga gcctcacccg accggtcctg
     5701 gtcgtcgcct tcgatggctg ggtggacggc ggccgcgccg gcacgctggc cgccgagaag
     5761 ctggccgacg acggccgcgt ggtggccacc ttcgacgacg accggctctt cgactaccgc
     5821 gcccggcggc cgaccctcga gatcgtggac ggccggctgc tggatctcga gtggcccgcg
     5881 ctgcgcgtga tggccaacca gtttcacggc cgcgacgtgc tgatcctcac cgggccagag
     5941 ccggactatc ggtggcgcgc cctcgccgct gacaccgtga agctggcgca ggagcttaga
     6001 gtggcgacct gggtcagtct cggcgccatc ccggccaccg tcccgcacac ccggccggtg
     6061 cccatcctgg gcaccgcatc ggtcgcagga ctgctgccgg agggggtccg acaggggccg
     6121 gacggacagc tgcgggtgcc gtcggccttc gtatcggtcc tggagcatgc ggcggcacgc
     6181 gccggcattc cggccctcgg cctgttcgcg caggtgccgc actatgcgtc cggtaccttc
     6241 ccgaccgcgg ccatcgagct gctcgcccgg ctgggcgtgt tcctcggcat cgagctgccg
     6301 ctgggtgacc tgcccacgcg ggcgctggag acgcggcagc tcctcgacgc ggccgtcagc
     6361 gccgaggagc gcacgcgcac ctacgtcgag cggctggagc agatggcgga cgaggcgcgg
     6421 ctgccggagg gagacgacct gatcgccgat atcgagcggt tcctgcgcga gggcggctcg
//
LOCUS       DATACA010000261         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2684690, whole genome shotgun sequence.
ACCESSION   DATACA010000261 DATACA010000000
VERSION     DATACA010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684690"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VHK28_05405"
     CDS             complement(<1..1138)
                     /locus_tag="VHK28_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141698.1"
                     /translation="MTSTLRSWAGTVGAGAWGEVSAGTRVLAVLMALLAGGLILVGQY
                     RPVEQSALVGVAVGTVSAVAAAITAYLAVTRPWWGLVAWLALIPMINLARAEVWLGPI
                     QLIGTTGGVVSLVVGVAAWRARLPVELRPVVPALAWWGLALGIVLAVLSTALAPLSAE
                     SVNITLHGLLEPFAVFGSVLALRPDARRAVQALAALAAGVVLAALVNLAYLFGTIGPA
                     SFYERRFLYARLTYFNVGIFSGMLVAAMVHLSTVLLARRHMAWPRWVAGAAWAGLAIE
                     MVAIFFTYTKSAWVAAATGLILVVLLLVKGWLRRAALLMAILALLAVVVPFPRPVLGA
                     ISPDLGDAYYDFVVSLQSEERVESWDPVSESGAGSVGIRFVALSA"
     gene            complement(1135..>1935)
                     /locus_tag="VHK28_05410"
     CDS             complement(1135..>1935)
                     /locus_tag="VHK28_05410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2141699.1"
                     /translation="AARARGLPVGIYFRDAYQLFRDLYPLRGWRARLSDLAWRLTLGP
                     MRTVASVHFVQSRDMASVLGLARPALLPPGTDPSAPDLGAGDGPVVAHVGAMNPADGF
                     DRLLAAMEIVRRSVPDARLLAIGPPPAVAAALPDWVDVRRATRDELAPLLADARVCVI
                     PRPITAYTHIVRPVKLSDYLAYGKPVVATDTREIRAVLEPTDAGLLVDDAPEAIAGGL
                     LRVLREPGLAEELAARSRALATDASMTWDGRAADILRALGLAAEGAEA"
BASE COUNT          331 a          717 c          689 g          198 t
ORIGIN      
        1 cggcgctgag cgccacgaag cggatgccca ccgatccggc gcccgactcc gagacggggt
       61 cccacgactc gacccgctcc tcagactgga gcgagacgac gaagtcgtag taggcgtcgc
      121 cgagatcggg cgagatggcg cccagcaccg gccgcgggaa tgggaccacg accgccagca
      181 gggcaaggat ggccatcagc aacgccgcgc gccgcagcca gcccttgacc aggagcagca
      241 cgaccaggat caggccggtg gccgccgcca cccaggcgct cttggtgtag gtgaagaaga
      301 tggcgaccat ctcgatcgcg agcccggccc aggccgcgcc agccacccag cgcggccagg
      361 ccatgtgccg gcgggcgagc agcacggtcg acaggtggac catggccgcc accagcatcc
      421 cgctgaagat gcccacgttg aaatacgtca accgggcgta caggaagcga cgttcgtaga
      481 agctggccgg accgatggtg ccgaacaggt aggccaggtt caccaatgcc gccagcacca
      541 cgccagcggc cagagcggcg agcgcctgga ccgcgcgacg cgcgtccggc cgcagggcga
      601 ggacgctgcc gaacacggcg aaaggttcca gcagcccgtg cagcgtgatg ttcacgctct
      661 ctgcggagag cggcgccagc gcggtgctca gcaccgccag gacgatgccg agggccagtc
      721 cccaccaggc gagggccggc acgaccggac gcagctcgac gggcaggcgt gcgcgccatg
      781 ccgccacccc caccaccagg ctgacgaccc cgccggtggt gccaatgagc tggatcggcc
      841 ccagccagac ctcggcgcgg gccaaattga tcatcgggat gagggccagc caggcgacca
      901 gcccccacca gggacgggtc accgccagat acgcggtgat cgccgcggcc accgcggaga
      961 ccgtgcccac ggccacgccc accagcgccg attgctccac cggccggtac tgcccgacca
     1021 ggatcagccc accggccagc agcgccatca gcacggcgag cacccgtgtc ccggcgctca
     1081 cctcacccca ggcgcccgcc ccgacggtgc ccgcccacga gcgcagcgtg ctggtcatgc
     1141 ctcggcaccc tccgccgcca ggccgagtgc tcgcaggatg tcggccgcgc ggccgtccca
     1201 ggtcatggag gcatcggtcg ccagcgcccg ggatcgggcg gccagctcct cggccaggcc
     1261 gggctcgcga agcacgcgca gcaggccgcc ggcgatcgcc tccggcgcgt cgtccaccag
     1321 cagcccggcg tcggtcggct ccaggacggc gcggatctca cgggtgtcgg tggccaccac
     1381 cggctttccg taggccaggt agtcgctgag cttcactggt ctcacgatgt gggtatacgc
     1441 ggtgatggga cgcgggatga cacagacccg tgcatcggcc aggagcgggg ccagctcgtc
     1501 gcgggtggct cgccgcacgt cgacccagtc gggaagggct gccgccaccg ccggcggcgg
     1561 gccgatggcg agcaggcgcg cgtccggaac gctccgccgc acgatctcca tggcggccag
     1621 cagccggtcg aagccgtcgg cggggttcat ggcgccaacg tgggccacga ccggcccatc
     1681 tcccgccccg aggtcagggg cggatgggtc agtccccggc ggcaggagcg cgggccgagc
     1741 caggcccagg acggatgcca tgtcgcgcga ctgcacgaaa tgcacgctgg ccaccgtgcg
     1801 catcggaccg agcgtcagcc gccaggccag atcggaaagg cgggcgcgcc agccgcgcag
     1861 cgggtagagg tcccggaaca gctggtaggc gtcgcggaag tagatgccca ccggcaggcc
     1921 ccgggcacgg gccgc
//
LOCUS       DATACA010000262         1590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_435659, whole genome shotgun sequence.
ACCESSION   DATACA010000262 DATACA010000000
VERSION     DATACA010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435659"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VHK28_05415"
     CDS             <1..489
                     /locus_tag="VHK28_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2141700.1"
                     /translation="FVGIAIAMPIILFHVWRFVTPGLTPGERRFVWPVILGGLLLFAL
                     GLTIGYLVIPYAITFLLGFAGDGLEPLLTVDEYIGFVTTMLLAFGLVLEFPILLIGLS
                     RVGILNYRRLAARRRWAILVIVLFAILLTPGGDPISPLILSGVMFILFEGSLQIIRLM
                     NR"
     gene            494..1447
                     /locus_tag="VHK28_05420"
     CDS             494..1447
                     /locus_tag="VHK28_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX2141701.1"
                     /translation="MPRVLLLTDPAMDAHAAPGHPERPERLGAVVDGVLDGTDAAGAE
                     LLREAPRPAGAETIERVHPERYVSSLDALAVRGGAWIDADTYLAPDSMAAARLAAGAT
                     VRAALAVAEGAATVAFAVVRPPGHHAARERAAGFCLLNNIAVAVEALRAAGAGQRLAI
                     VDWDVHHGDGTQEIYDADADLCYASTHQSPLYPGTGAAAERGSGQAAGTKHNVPLSPG
                     SGDAAFVEAWRDRLLPAIESFAPDALLVSAGYDAHRDDPLAHLEVTEAGYRDVAEGVG
                     ALARRLGLSGVALTLEGGYDLAGLRRSTTETVRGLLAGLGG"
BASE COUNT          186 a          586 c          572 g          246 t
ORIGIN      
        1 ttcgtgggca tcgccatcgc catgcccatc atcctgttcc acgtctggcg cttcgtgacc
       61 ccgggcttga cccccggcga gcgccgcttc gtgtggccgg tgatcctggg cggcctgctg
      121 ctgttcgcgc tgggcctgac catcggctac ctggtcatcc cgtacgccat caccttcctg
      181 ctcggcttcg ccggcgacgg cctggagccg ctgctgacgg tggacgagta catcggcttc
      241 gtgaccacca tgctgctggc cttcgggctg gtgctcgagt tcccgatcct gctcatcggc
      301 ctttccaggg ttgggatcct caactaccgg cgcctggccg ctcgccggcg ctgggcgatc
      361 ctggtcatcg tcctgttcgc catcctgctc acgccgggtg gcgatcctat ctcgccgctc
      421 atcctctcgg gcgtgatgtt catcctcttc gagggttcgc tccagatcat ccggctcatg
      481 aaccgctaga ccgatgcccc gcgtcctgct cctgaccgat ccggccatgg acgcgcatgc
      541 cgcaccgggg catcccgagc ggcccgagcg gctcggggcg gtggtggacg gcgtgctaga
      601 tgggaccgac gccgccggtg ccgagctgct gcgggaggcc ccgcggccgg ccggcgccga
      661 gaccatcgag cgcgtgcacc ccgagcggta tgtctcgtcg ctcgatgccc tggccgtacg
      721 cggcggtgcc tggatcgacg ccgacaccta cctggcgcct gactcgatgg ccgccgcccg
      781 gctggcggcg ggagccaccg tgcgcgcggc actggccgtg gccgagggcg cggccacggt
      841 cgccttcgcg gtcgtgcgac cgccgggcca ccacgcggcg cgcgaacggg ccgcaggatt
      901 ctgtttgctg aacaacatcg ccgtcgcggt ggaggcgctg cgcgccgcgg gggctgggca
      961 acggctggcc atcgtggact gggacgtgca ccacggcgac gggacgcagg agatctacga
     1021 cgcggacgcg gacctctgct acgcctccac ccaccagtct ccgctctatc cgggcaccgg
     1081 cgcggccgcg gaacggggga gcgggcaggc cgcgggcacc aagcacaacg tgccgctctc
     1141 gccgggcagc ggggacgcgg cattcgtcga ggcctggcgg gatcggctgc tgccggccat
     1201 cgagtccttc gcgccggatg cgctgctcgt ctcggcaggg tacgacgccc accgcgacga
     1261 cccgctggcg cacctcgagg ttaccgaggc cgggtatcgc gacgtcgccg agggcgtggg
     1321 cgcgctggcc cgccgcctcg gcctgagcgg cgtggcgctg acactggagg gcggctacga
     1381 cctggccggg ctgcggcgca gcacgaccga aaccgtccgg ggattgctcg cgggcctggg
     1441 cggatgagcg cgcgccaacc cggctgagcg tgcggtgggg ggatatactc ggggcgagca
     1501 tggcacgcgc cacccagtcc tccgtcgcgc cgcgcgaggc cgtccgacgg cggccccggc
     1561 tggccgcgcc tgccaccccc gcggcgctgc
//
LOCUS       DATACA010000263         5062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1882229, whole genome shotgun sequence.
ACCESSION   DATACA010000263 DATACA010000000
VERSION     DATACA010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882229"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..590
                     /locus_tag="VHK28_05425"
     CDS             117..590
                     /locus_tag="VHK28_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141702.1"
                     /translation="MTEVNGLRLAVRGLTGGLAAGYVWLAVTLLLAAPFGDPLAPHAR
                     LADWVLPASATREGSLLLGLSVAQLVGGAFGLLFAYFVGRFFTVRPTMLLAATCVALL
                     AWLAAGSATDPQLTAQIPMLVAVLVYGAVLGHALPLRDDVLRPMTTANASRQGLG"
     gene            complement(587..1177)
                     /locus_tag="VHK28_05430"
     CDS             complement(587..1177)
                     /locus_tag="VHK28_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2141703.1"
                     /translation="MPQRDATGTFPLTLGEEPSFCPACGERVEPRVLEADHMPRLVCP
                     RGHVTWRNPRVVVGTLPVRDGLVFLARRAIEPAAGSWSYPGGFLELGEAAQEGARRET
                     EEETELRVEVGRLIGAYSRPHAGIVTLIYEATVIGGEPLPAAETSEVRGFSPDEIPWD
                     ELAFTTTESALRDWVASLPGRGPRFAPELYVEGDPD"
     gene            1295..3091
                     /locus_tag="VHK28_05435"
     CDS             1295..3091
                     /locus_tag="VHK28_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141704.1"
                     /translation="MSVAAPAQRSAFSIFRNRSFTYLWTGQLVSTIGDALTSLAAGII
                     VFQITGSVLSVGLILMASAAPTLVFGLIAGVVVDRYDRKKIMIVTLVVRAGLVALIPF
                     LVMDGDDIIWLYVLVLASASLQQFFDPANDSVLPEVATDEELASANSLMAIAQFGSTA
                     IGFALAGLITAQFNVAVAFWLDAVTFLFSAVMIAMVKIGPIVAEELSNVRDVIRNLGF
                     GAGFIWRTPVLRSMLLLRVPVMLIIGLQNVLLLPFAFEVLNAGEFEYGLQEGITSIGF
                     VVGSLLMARYADRLREGSWLVLSFLGMGVAGLAYALSSNIWIAIGLIGISGILNAPSF
                     VAGRLINQRATTREVRGRVFSTSYVLRDVVYIGGMALAGLADIIDVRILFAVSSVALI
                     VTSLIGAFMPGIGQPAADWRRTMTLLRSAPRPAPGAARPATLADIEALGRYIPALAGM
                     PQRDQDTLVSHARVIDAEAGTAIMRAGEEGDSAFFILEGRAVAGAAGAEGEYRSLSSM
                     GIGDVFGEIAALTGSRRTADVVTEEPSSLLEVPADTLRNLMAIPEFGNLVLGKMQERL
                     SRSTTIGDLPRFGHFDHTALRKARAEAVAEAD"
     gene            3282..4679
                     /gene="cysS"
                     /locus_tag="VHK28_05440"
     CDS             3282..4679
                     /gene="cysS"
                     /locus_tag="VHK28_05440"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2141705.1"
                     /translation="MPPTIKLYNSLTRRLEPLEPVNPGRVAIYTCGSTVYRYAHIGNL
                     RTYLFGDLLRRVLEYLGYEVTYVKNITDVGHMRETGEDPILLAAEVEGKTPDQIADFY
                     TDAWLEDERLLNMRPAEVMPKATDHIPEMIAMTQTLLERGLAYEVNGNVYFDVSELPS
                     YGQLSGQKLDQMRSGHRVDVEPDKRDAEDFALWKRAEPGRLMKWRSPWGEGFPGWHIE
                     CSAMSMKYLGERFDIHTGGIDLKFPHHEDEIAQSEGVTGHRVVSIWMHGEFLTLADAK
                     MAKSAGNVIRVSDLPQKGFEPLVFRYLALQAHYRSKLDFTEEAMHGAASGLRRLRRAA
                     SEGDSAGTDVDLSVEPAAAYHERFVAAISDDLAMPAALAAAHAVAAANDLAPAQRRGL
                     LLDFDRVLGLGLDAPPDAALTELPAGAAELLERRAAARAARDFASSDALRDELAAMGV
                     EVRDTPDGQVTTVRR"
     regulatory      4809..4898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          737 a         1799 c         1719 g          807 t
ORIGIN      
        1 gccgctacga gtaggcgccc aggccgtcgg ccgcgctgac cctcgccgcg cgtaccctgc
       61 cgggccggac cttcggggct ctcggaaggt ccccggctca gcagtaggct cggcaaatga
      121 ccgaggtgaa tgggctgcga ctggcggttc gcggcctgac cggcgggctg gcggctggct
      181 acgtgtggct ggcggtcacg ctgctcctgg cagcgccctt cggcgatccg ctcgcaccgc
      241 acgcgcggct cgccgactgg gtgctgccgg cgagcgccac ccgggagggt tcactgctgc
      301 tgggcctctc ggtggcgcag ttggtgggtg gcgccttcgg cctgctgttc gcctacttcg
      361 tcggccgctt cttcaccgtg cggccgacca tgctgctggc cgcgacctgc gtcgcgctcc
      421 tggcctggct ggcggccggg tccgccaccg atccgcagct gaccgcccag atcccgatgc
      481 tggtggcggt gctcgtgtac ggcgcggtcc tcggccatgc gctgcccctg cgggacgatg
      541 tcctccgacc catgaccacg gctaacgcct cccgtcaggg tctcggctag tccggatcgc
      601 cctcgacgta cagctccggc gcgaagcgcg gcccccggcc agggagcgag gccacccagt
      661 cgcgcagcgc gctctcggtt gtggtgaagg ccagctcgtc ccagggaatc tcgtccggcg
      721 agaagccgcg cacctctgag gtctcggccg ccggcagcgg ctcgccgccg atcacggtgg
      781 cctcgtagat cagggtcacg atgcccgcgt gcgggcggga gtaggcaccg atcaggcgcc
      841 ccacctccac ccgcagctcg gtctcctcct ccgtctcacg ccgcgcgccc tcctgcgcgg
      901 cctcgccgag ctccaggaag ccaccggggt agctccagct gccggccgcg ggctcgatcg
      961 cgcgccgggc gaggaacacc agcccgtcac gcaccggcaa ggtgccgacc accacccgcg
     1021 ggttgcgcca ggtgacatgg ccgcgcgggc agaccaggcg aggcatgtga tccgcctcca
     1081 gcaccctggg ctcaacccgc tccccgcagg cgggacagaa ggacggctcc tcgccgaggg
     1141 tcagcgggaa ggtgccggtc gcgtcgcgct ggggcatgcc gggatggtag atggttgaca
     1201 gcgcgggatc gctcgccggt gccacaatgc gcccgcgaat cccgagccac ggtccgccgc
     1261 ccgtcgccca agcggatgtc accggaggaa caggttgtcc gtagccgccc ccgcccagcg
     1321 ctccgccttc tccatcttcc gcaatcgctc gttcacgtat ctgtggaccg gacagctggt
     1381 ctccaccatc ggcgatgcgc tgaccagcct ggcggccggc atcatcgtct tccagatcac
     1441 cggctcggtg ctgtcggttg gcctgatcct gatggcgtcg gccgcgccca cgctggtctt
     1501 cggcctgatc gcgggcgtgg tggtggatcg ctacgaccgc aagaagatca tgatcgtcac
     1561 cctggtggtg cgggccggcc tggtggcgct catcccgttc ctggtcatgg acggcgacga
     1621 catcatctgg ctgtacgtcc tggtgctggc ctccgcctcg ctgcagcagt tcttcgaccc
     1681 tgccaacgac agcgtcctcc ccgaggtggc taccgatgag gagctggcca gcgccaactc
     1741 gctgatggcc atcgcgcagt tcggctccac cgccatcggc ttcgcgctgg ccgggctgat
     1801 cacggcccag ttcaacgtcg cggtcgcatt ctggctggac gccgtgacct tcctcttctc
     1861 ggcggtgatg atcgccatgg tcaagatcgg gcccatcgtg gccgaggagc tgagcaacgt
     1921 ccgtgacgtc atccgcaacc tgggcttcgg ggccggcttc atctggcgca ccccggtcct
     1981 gcgctccatg ctcctgctgc gcgtcccggt catgctcatc atcggcttgc agaacgtgct
     2041 gctgctgccc ttcgccttcg aggtgctaaa cgccggggag ttcgagtacg ggctgcagga
     2101 gggcatcacc tccataggct tcgtggtcgg cagcctgctc atggcccgct acgcggaccg
     2161 actgcgcgag ggatcgtggc tggtcctcag cttcctgggg atgggcgtcg ccgggctggc
     2221 ctacgcgctg tcctcgaaca tctggatcgc gatcggcctg atcgggatca gcggcatcct
     2281 caacgcgccg tcgttcgtgg ccggccgcct catcaaccag cgagccacca cccgcgaggt
     2341 gcgcggccgc gtgttcagca cctcctacgt cctccgcgac gtggtctaca tcgggggcat
     2401 ggcccttgcc gggctggcgg acatcatcga cgtgcgcatc ctgttcgcgg tcagctcggt
     2461 ggcgctgatc gtcacctcgc tgatcggtgc cttcatgccg ggcatcggcc agccggcagc
     2521 cgactggcgg cgcaccatga ccctgctgcg ctctgcgcct cgaccggccc ccggcgcggc
     2581 ccgcccggcg acgctggccg acatcgaggc gctgggccgc tacatcccgg cgctggccgg
     2641 catgccgcag cgggatcagg acacgttggt cagccatgct cgtgtaatcg atgcggaagc
     2701 gggcacggcc atcatgcgcg ccggcgagga gggcgacagc gccttcttca tcctggaggg
     2761 gcgggctgtg gccggcgccg ccggagcgga gggcgaatac cgctcgctgt ccagcatggg
     2821 gatcggcgac gtcttcggcg agatcgccgc gctgaccggc agccggcgta ccgccgacgt
     2881 ggtcaccgaa gagccgagca gcctgctgga ggtgcccgcc gacacgctcc gcaaccttat
     2941 ggccatcccc gagttcggca acctcgtgct cggaaagatg caggagcggc tctcgcgctc
     3001 aacgacgatt ggtgacctgc cgcgcttcgg ccacttcgac cacacggccc tgcggaaggc
     3061 acgcgcggag gccgtcgccg aggccgactg acgcgctgca cttcccaaat tcccgatgcg
     3121 ttacggtcgc caggcggccg gatgagggcc ggtcggggcg cggctgagcg caacgcgcca
     3181 ccgattcggc cacgctgacg cgcataacgc ttctcccggg cgcataccgg cagtcgggca
     3241 cctgctgagg tcgagaagcg catcggtaca atgccggtcg catgccaccg accatcaagc
     3301 tctacaactc cctgacccgc cgcctcgagc cgctcgagcc ggtcaatccc ggccgggtcg
     3361 cgatctatac ctgcggcagc acggtctacc gctacgcgca catcgggaac ctgcggacct
     3421 acctgttcgg cgacctgttg cgccgggtgc tcgagtacct ggggtacgag gtcacctacg
     3481 tcaagaacat caccgacgtg ggccacatgc gtgagaccgg cgaggacccg atcctgctcg
     3541 ccgccgaggt cgagggcaag acgccggacc agatcgccga cttctacacc gatgcctggc
     3601 tggaggacga gcgcctcctg aacatgcggc ccgcggaggt catgcccaag gctaccgacc
     3661 acatccccga gatgatcgcg atgacccaga cgctgctgga gcgcggcctg gcctacgagg
     3721 tgaacgggaa cgtctacttc gacgtcagcg agctgccgtc ctatgggcag ctctccggac
     3781 agaagctgga ccagatgcgc tccggccacc gggtggacgt cgagccggac aagcgcgacg
     3841 cagaggactt cgcgctctgg aagcgggcgg agccgggccg gctcatgaag tggcgttcgc
     3901 cgtggggcga gggcttcccg ggctggcaca tcgagtgctc ggccatgtcg atgaagtacc
     3961 tcggcgagcg attcgacatt cacaccggcg ggatcgacct gaagttcccc catcacgagg
     4021 atgagatcgc acagtcggag ggcgtgaccg ggcaccgcgt ggtcagcatc tggatgcacg
     4081 gcgagttcct gaccctggcg gacgccaaga tggcgaagtc ggccggcaac gtgatccgcg
     4141 tcagcgacct gccgcagaag ggtttcgagc ccctcgtctt ccgctacctg gccctgcagg
     4201 cgcactatcg gtccaagctg gacttcaccg aggaagccat gcacggtgcg gcgtcagggc
     4261 tgcgtcggct gcgccgcgcc gcttccgaag gcgactccgc cgggaccgat gtcgacctgt
     4321 cggtcgagcc ggcggccgcc taccacgaac gattcgtggc cgccatctca gatgacctgg
     4381 ccatgcccgc cgcactggcc gctgcccacg cagtggccgc cgccaacgat ctggctccgg
     4441 cccagcgccg cggcctgctg ctcgatttcg accgcgtact ggggctcggc ctggacgcgc
     4501 cccccgacgc cgcgctcacc gagctgccgg ccggcgccgc cgagctgctg gagcgccgtg
     4561 ctgcagcgcg cgcggcgcgg gacttcgcct cgtccgatgc gttgcgcgac gagctggcgg
     4621 ccatgggcgt cgaggttcgg gacaccccgg acggacaggt caccacggtc cgtcggtagt
     4681 gcgctgcgcg tcgctcccgc gcggttgact gtagtgcgga ccactacctg cggcctggac
     4741 ccgagtacgg ataggcgatg gcgcgcagag ccccagtgcc cgtagggttc tcccaccgcg
     4801 cggaacgcgg acgacaagag caaacccggg gtgacccggg gacgcaatgc cacgggactg
     4861 gttcaagagg ggcggctcca gtcagccggg ttgccgaacg gcggcggcaa gccgggcggt
     4921 ggggggaacg gtgacgactc gagctccagc ctagctctag tcatcgtgag ctctgccgac
     4981 tcgaccgtaa gctatggcga cgtggtcact ttcagggtgt ccacgacatc gacggacaag
     5041 ccgttcgtca cattgaactg ct
//
LOCUS       DATACA010000264         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_435732, whole genome shotgun sequence.
ACCESSION   DATACA010000264 DATACA010000000
VERSION     DATACA010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435732"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VHK28_05445"
     CDS             <1..414
                     /locus_tag="VHK28_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX2141706.1"
                     /translation="TSDMARSLRSQADAAGIPSISAEMGESRRVTKEYVPIGVRGVHH
                     VLRHLGIEPGEAEANKEPREFRTITLVHAQQGGGLRWSADLTDDVRAGQPIADVVDVF
                     GDTVETLQAPVDGFILRKMLFGSLATGAEVAWIAS"
     gene            404..1387
                     /locus_tag="VHK28_05450"
     CDS             404..1387
                     /locus_tag="VHK28_05450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141707.1"
                     /translation="MRADLPTLGVRLPNSGPFATPDALLETAELAERLGYERVWVHDH
                     ISWSRDKLTHFATGSLEACADQDPNFFESVTTSAVLAGRLRRIGVGIAGLVIPLRDPR
                     ILAKQLAITDALSGGRLTVALAIGNVRGDFEVMGVPFERRGKLSNEYLGALRAILRGE
                     QPVSYQGETISFTDGTFLPQPKNLRLWVTGSSDPGLRRAARFADGWMTVYQSTEAYRA
                     FVEQLGAFAQEEGRSPDDLEKAYETYVCVAATQDEAVAVARRSLEGKFKTLDAGLDVC
                     IVGDPDGFLERCAEYRAAGAGHLELKFIGHDVGQLHEEMAILAEAAGLRNA"
     gene            1384..2034
                     /locus_tag="VHK28_05455"
     CDS             1384..2034
                     /locus_tag="VHK28_05455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX2141708.1"
                     /translation="MSYASIEPASAALVFFDTLNYGFANPPDDHRDRVEAVKANWVRL
                     AEAARSHGVPLVFPMADHRPDGADTERRYTDVNHDMQPWPDPEVRFRPHTANTSGSWG
                     AQVIDELSPRPEDYLIPKHRWSAFFQTHLELSLRSRGANTIILCGGATEIGIASTAYA
                     ARDLDFDLVIVRDATTSRRQHVHDLFVNDVFPRFARVRTTDQVVAMLDAGADSPQS"
     gene            2039..3049
                     /locus_tag="VHK28_05460"
     CDS             2039..3049
                     /locus_tag="VHK28_05460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141709.1"
                     /translation="MPTTRIGLDLKHEGRRFQLSGRDMIELAVAAEMAGVESVWTNED
                     IGFDSFAVLSAIAQHTQRIRLGTAIVNVYNRSAMQLAMGVATLDELSAGRAILGLSIG
                     HHPWNDLGHGIPIEQPLSRLREYVAFLRKALSGEPFRHDGSVFHGVDTQLAFDPVRPA
                     IPIFIAGERPRIVQLAGEVADGLLINVVSPEYIANVAADRFFSSARASGRNAEQLELT
                     ALVTCAVDDDPAAALVRARRMVVHRLRHSLKMLDTQPAQRHDEIRHLHALMQAGERDR
                     AAEEVSEDLARSIVVAGGPQDVAAGLQRYLAAGCSRVIAVSYPRARPDVARLIAALRP
                     VL"
BASE COUNT          497 a         1218 c         1066 g          501 t
ORIGIN      
        1 accagcgaca tggcccgcag cctgcgcagc caggccgatg ccgccggcat cccgtccatc
       61 tctgcggaga tgggggagtc gcgccgggtg acgaaggagt acgttcccat cggcgtccga
      121 ggcgtgcacc atgtgctgcg ccacctgggc atcgagcccg gggaggcgga ggcgaacaag
      181 gagccgcgcg agttccgcac catcacgctc gtccacgccc agcagggtgg aggtctgcgc
      241 tggtcggccg acctgaccga tgacgtccgc gccggccagc cgatcgccga tgtggtcgat
      301 gtcttcggcg acacggtcga gacgctgcag gcgccggtgg acggcttcat cctgcgcaag
      361 atgctgttcg gatcgctcgc gaccggagcg gaggtggcct ggattgcgag ctgacctgcc
      421 gacactcggc gtgcggctgc cgaactccgg cccgttcgcc acgccagacg ccctgctcga
      481 gacggcggag ctcgccgagc ggctgggcta cgagcgggtc tgggtccacg accacatctc
      541 ctggtcccgc gacaagctca cccacttcgc caccggttcg ctggaggctt gcgccgacca
      601 ggacccgaac ttcttcgaga gcgtcaccac cagcgccgtg ctggccgggc gactgcggcg
      661 catcggggtg gggatcgccg ggctggtcat ccccctgcgc gacccgcgca tcctggccaa
      721 gcagctggcg atcaccgacg cgctcagtgg cggtcgcctc accgtggcac tggccatcgg
      781 caacgtccgc ggcgacttcg aggtcatggg cgtgccgttc gagcgccgtg gcaaactctc
      841 gaacgaatac cttggtgcgc tgcgcgcgat cctccgcggc gagcaaccgg tctcctacca
      901 gggcgagacg atctcgttca ccgatggcac attcctcccg caacccaaga acctgcgcct
      961 gtgggtcacc ggctcctccg accccggcct gcgccgcgcc gcacgcttcg ccgacggctg
     1021 gatgaccgtg tatcagagca cggaggcgta ccgcgccttc gtcgagcagc tgggcgcctt
     1081 cgcccaggag gagggccgct cgccggacga cctggagaag gcgtacgaga cctacgtgtg
     1141 tgtggccgcc acccaggacg aagcggtggc cgtcgcccgc cgctccctgg aaggcaagtt
     1201 caagaccctc gacgcgggcc tcgacgtgtg catcgtgggc gacccggacg gcttcctgga
     1261 gcgctgcgcc gagtaccgcg ccgccggggc aggtcacctc gagctcaagt tcatcggcca
     1321 cgacgtcggg cagctgcacg aggagatggc catcctcgcc gaggcagccg ggctgcggaa
     1381 cgcgtgagct acgccagcat cgagccggca tctgcggccc tcgtcttctt cgacacgctc
     1441 aactacggct tcgccaaccc gcccgacgat catcgggacc gcgtggaggc ggtcaaggcc
     1501 aactgggttc gcctggcgga ggcggcccga tcccacggcg tgccgctcgt cttccccatg
     1561 gccgaccacc ggccggacgg cgcggacacc gagcgccgct acacggacgt caaccatgac
     1621 atgcaaccct ggccggaccc ggaagttcgc ttccggccgc acaccgcaaa cacctccggc
     1681 agctgggggg cgcaggtcat cgacgagctg agtcctcgtc cggaggacta cctgatcccc
     1741 aagcaccgat ggagcgcttt cttccagacc cacctcgagc tcagcctgcg gtcgcgcggg
     1801 gctaacacca tcatcctgtg cggcggtgcc accgagatcg gcatcgccag cacagcctac
     1861 gccgcccgcg acctcgactt cgacctggtc atcgtgcgcg atgccactac ctcgcgccgg
     1921 cagcacgtcc acgatctctt cgtgaacgac gtcttccccc ggttcgcacg ggtgcgcacg
     1981 accgaccagg tggtcgccat gctcgacgcg ggcgcagaca gcccccagtc ctagcccgat
     2041 gccgacgacg cgcatcggcc tcgatctcaa gcacgagggc cgccggttcc agctgagcgg
     2101 ccgcgacatg atcgagctgg ccgtggccgc cgagatggct ggcgtcgaga gcgtctggac
     2161 caacgaggac atcggcttcg actcgttcgc cgtcctgtca gccatcgcgc agcacacgca
     2221 gcgcatccgg ctgggcacgg ccatcgtgaa cgtctacaac cgatccgcca tgcagctggc
     2281 catgggcgtc gccaccctgg acgagctgtc cgctgggcgg gccatcctgg ggctaagcat
     2341 cggtcatcac ccgtggaacg acctgggcca cggcatcccg atcgagcagc cgctctcccg
     2401 gctccgggag tacgtggcct tcctgcgcaa agcgctcagc ggcgagccgt tccgccacga
     2461 cggctcggtg ttccatggtg tggataccca gcttgcattc gaccccgtgc gtcccgcgat
     2521 cccgatcttc atcgccgggg agcgaccgcg gatcgtgcag ctggccggcg aggtcgccga
     2581 cggcctgctg atcaacgtgg tcagccctga gtacatcgcg aacgtcgccg cggaccgctt
     2641 cttctccagc gcccgcgcct cgggccgcaa cgcggagcag ctggagctca cggcgttggt
     2701 gacctgcgcc gtcgacgacg acccggcagc cgccctggtc cgcgcacgac gcatggtcgt
     2761 ccaccgcctg cgccactcgc tgaagatgct cgatacccag cccgcgcagc gccacgacga
     2821 gatccgccat ctccacgccc tgatgcaagc cggcgagcgc gaccgcgccg cggaagaggt
     2881 cagcgaggac ctggcccgct ccatcgtggt ggccggcggg ccgcaggacg ttgcggctgg
     2941 cctgcagcgc tacctggctg ccggctgcag ccgggtgatc gcggtttcct accctcgcgc
     3001 ccgcccagac gtcgcacgcc tgattgctgc gttgcgccca gtgctctgat gcggcacggt
     3061 cctcgccgtg gccgcgggtt gggaatccca tcgaggcatg cctaacttcg tgttatcctc
     3121 acggccatgc agcgtcgcgc cacgcgtccc atcgccgaca tgagcctccc gtgctcttgt
     3181 ccccaggcgg gagccgactg gcccgcctga gcgcctcgct tgctgcctca cccataggtc
     3241 tctgctcccg cactccccgc ctcgtggctg ctgctttgcg cc
//
LOCUS       DATACA010000265         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2042883, whole genome shotgun sequence.
ACCESSION   DATACA010000265 DATACA010000000
VERSION     DATACA010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042883"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VHK28_05465"
     CDS             <1..420
                     /locus_tag="VHK28_05465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2141710.1"
                     /translation="HSRLRPGGRLLNHCITRPDNRTRARAGSFIDRYVFPDGELTGSG
                     TIITAMQDVGFEIRHEENLREHYARTCAAWCANLVEHWDECVAEVGEATAKVWGLYLA
                     GSRLGFEGNDIQLHQVLGVRLHADGRADFPLRPDWER"
     gene            924..>1806
                     /locus_tag="VHK28_05470"
     CDS             924..>1806
                     /locus_tag="VHK28_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2141711.1"
                     /translation="MHIDQLATNAARQVIVPLDPIAEALKLTEGDFHPTVWNSGTYNG
                     QRYGIPLDIHPLGFYYNNAHMQQAGVQQPPTDADSWAQTIQALEQGGVETPFWVTATW
                     PAHLMFFSLINQFGGSMFNEDATQATFASPEGIEALEWYTSWIQQGASPRNVAEDAQA
                     TAFKQGRNSLTWDGIWMMNEWDTVSSLDWGVAPVPTIGDQPAVWASSHNFVVMRQPET
                     DQNKLNAARVFISWISENSIEWAKSGQIPARNRVRESQEFQQLDVQSTLAEQLDNVVF
                     PPAVPGIGDITLPTWETA"
BASE COUNT          312 a          631 c          592 g          271 t
ORIGIN      
        1 cacagccggc tgcgccccgg tggcaggctg ctgaaccact gcatcacccg gcccgacaac
       61 cggacccggg cacgcgccgg cagcttcatc gaccgctacg tgttccccga cggcgagctg
      121 accggctcgg gcacgatcat caccgcgatg caggacgtcg gcttcgagat ccggcacgag
      181 gagaacctgc gcgagcacta cgcccgcacc tgcgccgcgt ggtgcgcgaa cctggtggag
      241 cactgggacg aatgcgtggc cgaggtcggc gaggccacag ccaaggtgtg gggcctgtac
      301 ctggccggct cgcggctggg tttcgagggc aacgacatcc agctgcacca ggtgctgggc
      361 gtccggctgc acgccgacgg ccgggccgac ttcccgctgc ggcccgactg ggagcgttag
      421 acccacggtt tcggggtacc gctgtgacgg cggagcagcc gggggaaatt cgcatgtttg
      481 tttctgttgt gagcggcttg cgaacctggg tcttgctgtt cggttgtgtt tcgttctagt
      541 ctccgaacac ttcccgcacc caggaggcgt gatgaccggc gtaccgaacc tctcccgttc
      601 ttccttcgag ctcgaccgcc gcgggttcct gcggctcggt ggcctcgcgt cggccgccgt
      661 cctcctcaac gcctgtggcg gcgggtcgtc gcagcccacg tcgcaggact tcggcagcgg
      721 caagacctac gacggcccca aggtcacgct ggagttctgg aacggcttca ccggcggcga
      781 cggcccgttc ctgcgcaagc tggtcgagca gttcagcaag gaacagaaga acatcgacgt
      841 caacatgacg gtcatggagt gggaggacta ctacgggaag gtcccgaacg ccgtggccag
      901 cggcaacggc cccgacgggc atcatgcaca tcgaccagct cgccaccaac gccgcgcgcc
      961 aagtcatcgt cccgctcgac cccatcgccg aggcgctcaa gctcaccgag ggcgacttcc
     1021 acccgacggt ctggaactcc ggcacctaca acgggcagcg ctacggcatc ccgctcgaca
     1081 tccaccccct gggcttctac tacaacaacg cgcacatgca gcaggccggc gtgcagcagc
     1141 cgcccaccga cgccgactcc tgggcacaga cgatccaggc gctggagcag ggcggtgtgg
     1201 agacgccgtt ctgggtcacc gcgacctggc cggcgcacct gatgttcttc tcgctgatca
     1261 accagttcgg cgggagcatg ttcaacgagg acgccaccca ggcgacgttc gcctccccgg
     1321 agggcatcga ggccctggag tggtacacca gctggatcca gcagggcgcc agcccgcgca
     1381 acgtcgccga ggacgcccag gcgacggcgt tcaagcaggg ccgcaactcc ctcacctggg
     1441 acggcatctg gatgatgaac gagtgggaca cggtcagcag cctcgactgg ggcgtggcgc
     1501 cggttccgac gatcggtgac cagcccgcgg tctgggccag ctcgcacaac ttcgtggtga
     1561 tgcgccagcc cgagaccgac cagaacaagc tcaacgctgc ccgcgtgttc atctcctgga
     1621 tcagcgagaa ctcgatcgag tgggcgaagt ccgggcagat ccccgcgcgc aaccgcgtcc
     1681 gcgagagcca ggagttccag cagctcgacg tgcagtcgac gctggccgag cagctcgaca
     1741 acgtcgtctt cccgccggcc gtcccgggca tcggtgacat cacgctgccc acgtgggaga
     1801 ccgcgg
//
LOCUS       DATACA010000266         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1640458, whole genome shotgun sequence.
ACCESSION   DATACA010000266 DATACA010000000
VERSION     DATACA010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640458"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VHK28_05475"
     CDS             <1..264
                     /locus_tag="VHK28_05475"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2141712.1"
                     /translation="TAVAVSKASFTTADVVYVATGANFPDALAGGPVAGMGDAPLLLV
                     PGTSVPPSVKQELLRLNPDKVVVLGSTGVVSQGVVFQLRMLFP"
     gene            397..>2513
                     /locus_tag="VHK28_05480"
     CDS             397..>2513
                     /locus_tag="VHK28_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2141713.1"
                     /translation="METTTRRSLRLVALALWLAAVLATGALVPVRATDPASNLPPAPE
                     PLTPGVHGEWLSDAPELPADVVADPQAEAGSVESLGSTFSTSAVPRLPNGLMREVMGY
                     LPYWELSTADIDAVRYDLVSTIAYFGVSARGDGTLARTAGGQPTAGWSGWTSSALSTV
                     IQRAHARDVRVVLTVTMMDWTGTYTAMSQLLNSSSNRARLVAEMASVLKARGADGVNL
                     DFEPVPTSLKSQYTSFVRQVKAGLAQAGAGSYVTVATMGGAASWATGYDLLGLTAPGA
                     ADAIMVMGYDFSWSGSARAGGVAPISNPYILDISEAMNAHLALGVPPSKMIWGVPYYG
                     RSWPTQNDQLNARTCTSTSVCPGAKALAPGASSSMTYVGARDLAAQKGRRWDATGKVP
                     WMAWYDSTNTVWKQAYYDDTASLGAKYDLVRSKGLSGIGIWALGMDTGRSELWDLINA
                     RFLRRDTRLAGADRYATAAAISRHGFPTGAPVVYLATGGDFPDALAGGVSAAQAGGPI
                     LLAKRDELPAATAAELARLKPQRVVVLGGTGVVSDAVANAAAAYATDKVPQRLAGANR
                     YATAAAVSRDAFPTGAAVAYLVTGGNYPDALAGGVLAARKGGPVLLVTPNTLPGATAA
                     ELDRLNPQTVVVIGGTAAVSDAVLNGAGKYATSGQLPQRIHGSDRWSTAVAISKAAFP
                     TAPDRAFVATGVAFPDALAAVPVA"
BASE COUNT          363 a          892 c          916 g          342 t
ORIGIN      
        1 accgcggtgg ccgtcagcaa ggccagcttc acgaccgcgg acgtggtgta cgtggcaacc
       61 ggggcgaact tcccggatgc gctggccggg ggcccggtgg ccggcatggg agacgccccg
      121 ttgctgctgg tgcctggcac atccgtgcca ccgagcgtca agcaggagct gctgcggctg
      181 aaccccgaca aggtcgtggt cctgggcagc acgggagtgg tgagccaggg tgtggtgttc
      241 cagctgcgga tgctgtttcc ctagccgtgt ttccggacgc ggtcccggga gagagtccat
      301 aggtaccatt ccggcggtcg ccgcggtccg gtagggtgcc cggtggtgcg gtctgaggtg
      361 gaccgattcg ctccaggagg ggaaggaggc cgagcggtgg agacgacgac gcggcgatcg
      421 ctgcgacttg tggccctggc attgtggctg gctgccgtgc tggccacggg agccctggtg
      481 ccggtgcgcg ccacggatcc ggcctcgaac ctgcccccgg cgccagagcc gctgacaccc
      541 ggcgtccacg gggagtggct gtccgacgcg cccgagctgc cggccgacgt ggtcgccgat
      601 ccgcaggccg aggcgggcag cgtcgagtcg ctgggcagca ccttcagcac cagcgcggtc
      661 ccacgcctgc ccaatggcct gatgcgcgag gtgatgggat acctgcccta ctgggagctc
      721 agcaccgcgg acatcgacgc ggtgcgttac gacctggtct cgaccatcgc ctacttcggc
      781 gtatcggccc gcggcgacgg gacgctggcg cgaacggccg gcggccagcc gacggcgggc
      841 tggtccgggt ggacgtcgag cgccctcagc accgtcatcc agcgcgccca tgcgcgcgac
      901 gtgcgagtgg tgcttaccgt gaccatgatg gactggaccg gcacgtacac cgccatgtcg
      961 cagctgctca acagcagctc caaccgcgcc aggctggtcg ccgagatggc gagcgtgctg
     1021 aaggcgcgcg gcgcggacgg cgtgaacttg gacttcgagc cggtgcccac cagcctgaag
     1081 tcgcagtaca ccagcttcgt ccgccaggtt aaggccggtc tggcccaggc cggcgccggg
     1141 tcctacgtca cggtcgcaac catgggcggc gccgcgtcgt gggccaccgg ctacgacctg
     1201 ctgggactga ccgcgcccgg ggccgccgac gccatcatgg tcatgggcta cgacttcagc
     1261 tggagcggct cggcgcgggc cggaggcgtg gcgcccatct ccaacccgta catcctggac
     1321 atcagcgagg cgatgaacgc ccacctggcg ctcggcgtgc cgccctccaa gatgatctgg
     1381 ggagtgccgt actacggccg ctcatggccg acccagaacg accagctcaa cgctcggacc
     1441 tgcaccagca ccagcgtctg cccgggcgcc aaggccctgg cgccgggtgc ctcgtcgagc
     1501 atgacctacg tcggggcgcg agacctggca gcccagaagg gcaggcgctg ggacgccacc
     1561 ggcaaggtcc cgtggatggc ctggtacgac agcaccaaca ccgtctggaa gcaggcctac
     1621 tacgacgaca cggcctcgct gggcgccaag tacgacctgg tccgctccaa gggcctgtcc
     1681 gggatcggga tctgggcgct ggggatggat accggccgtt ccgagctgtg ggacctgatc
     1741 aacgcccgct tcctgcggcg cgacacacgg ctggccggcg ccgaccgcta cgcgacggcc
     1801 gccgccatca gccgtcacgg cttcccgacc ggcgcgccgg tcgtctatct ggcaaccggg
     1861 ggcgacttcc ccgatgcgct ggccggcggc gtgagtgcgg cccaggccgg cgggccgatc
     1921 ttgctggcga agcgcgacga gctgccggcg gcgaccgccg ccgagctggc gcgcctcaag
     1981 ccgcaacggg tggtggtgct gggcgggacc ggcgtggtca gcgacgccgt ggcgaacgcc
     2041 gcagcggcct acgccaccga caaggttccc cagcgcctgg ccggcgccaa ccgctacgcc
     2101 accgcggcgg cggtcagcag ggacgcattc cccacgggtg ccgcggtcgc ctacctggtc
     2161 accggcggca actaccccga tgccctggcc ggcggcgtgc tggccgcgcg caagggcggc
     2221 ccggtgctgc tggtgacgcc gaacacgctc ccgggcgcca cggcggcgga gctggaccgc
     2281 ctcaacccgc agacggtggt cgtcatcggc ggcaccgccg cggtcagcga cgcggtgctc
     2341 aacggagccg ggaagtacgc caccagcggt cagctcccac agcgaatcca tggctccgac
     2401 cgctggtcga cggcggtcgc catcagcaag gccgccttcc cgacggcgcc agatcgggcc
     2461 ttcgtggcta ccggcgtggc cttcccggac gcgctggccg ccgtgccggt ggc
//
LOCUS       DATACA010000267         4995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1640495, whole genome shotgun sequence.
ACCESSION   DATACA010000267 DATACA010000000
VERSION     DATACA010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640495"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VHK28_05485"
     CDS             complement(<1..413)
                     /locus_tag="VHK28_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2141714.1"
                     /translation="MPARELGIRIGLLPAGPLGAITDVPGLRVGHATLISGDGALRVG
                     HGPVRTGVTVILPRDAIGANPVFAGTHTLNGNGEMTGLEWVRESGMMTTPIGLTNTHS
                     VGEVRDALVSASVAGARPDRPRWSLPVVAETWDGRL"
     gene            complement(400..2094)
                     /locus_tag="VHK28_05490"
     CDS             complement(400..2094)
                     /locus_tag="VHK28_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141715.1"
                     /translation="MPAVHVPQRVGAVLGVARNASLRRVLLAFAGFSITEWMTWIAIL
                     VFAYARGGATETGIAAVVQLVPSALVAPVAAVLGDHVRRERALLIAYLAQTVAMAMTA
                     VALFAGSPGWVVYAFAALAATSITLTRPVQGAILPSLSRAPSELTAANVAVGLVENGS
                     LLIGPLLAGVLLVLVGPGTVFAAAAALTGGGALLVAGIRPLDVDEWVAERSRDGAGGL
                     LSEALGGFRTLAREPRPRAVLLLMTGAAVLWGALDVYLVVLAFDRLALGEAGVGYLNA
                     ALGLGGLVGAVITVGLIGRSALGGPLAIGLALWAIALAALGLVPVVAVAFALLAGAGV
                     GSVLIDVSGRTLLQRVAPGALLSRVFGVLEGVSAAGLAVGSALAPLLIALLGIVGALL
                     ASGVGLLALVAVSWRVLRDLDTVPVARPRELAILRSTTLFAPLSPPALERLAGNLRPV
                     HAHAGTVVIRQGEAGDHFYILTDGEVSVAVDGREVRRLAAGSSFGEIALLRNVPRTAT
                     VRALTGAELLALERHVFLEAVTGHAASSAAAERLVDEQLGQVAPVDTAEEGTHAGP"
     gene            complement(2116..2502)
                     /locus_tag="VHK28_05495"
     CDS             complement(2116..2502)
                     /locus_tag="VHK28_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141716.1"
                     /translation="MTTAIPGAGPGCLPRVAVVDADRRVQQSIADVLRIAGVDVVGTA
                     GDPALALQLAAQQRPAVVLVDPRLPDVAAGEAFLRALGRDFPEVRVVLMGWNQPAELQ
                     LRSDAFVAKSAGPEEFVAAAIEACRG"
     gene            complement(2565..>4995)
                     /locus_tag="VHK28_05500"
     CDS             complement(2565..>4995)
                     /locus_tag="VHK28_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2141717.1"
                     /translation="GGINGLAAASGQPAWTDDYANDERIPHDPDDDAPARLELGAVAV
                     APLRGVRGDVIGTLAITYREPRHIDPRDVALLEELAGQGAIAARNARLYQQLRERADA
                     LARSAEAQRTMAAIAAQISSIRDPGTVLRRTVDESKRLLGADHAVIDRFEPEIDLLRG
                     LSAEDLTPILADVHVEPGVGVAGAAIQSGEVRRTGDYLVDRGFVHNDEADAWMRGRGY
                     RSQMSAPLAGDSGPIGTLTVYSMRPNAFDEPDAELLGALASHAAIVQSNARLYAEARR
                     AAEDLAHLVEAQRTLSAIATQITSIRDPAVVLQRTVDEAHRLLGADMALINPLDSGSR
                     VLAWAVAHAPRDVPLDDVVVTPGEGVSGTSLSERRVVRTGDYLNDPAFPHGPELDDYI
                     RRRGMCSVMSAPLVAGGEPLGTLTVQAARTEAFSESDAQLLDALAAQAAVAITNARLL
                     EQLAESERRYRHLVDHSPDLVWSVDAEGHFTYLGEAVERMTGFRASELLGRHWETLVG
                     AESLEAARAAWRAVQERPEEDLQFRIVLPLSGGGSIPAEVNMVATLVDGRFAGVQGSI
                     RDVRERERLEEDLRRQAAALAAGEERANLARELHDSVTQALFSMGLTARALELVMERD
                     PAAARDKMRELRDLQKDALAEMRTLIFELRPQALETDGLAQALRNHGAAVQGRTGLAV
                     SVEVEYDERTALDVEEALYRVAQEALHNVVKHANAQNATIGLRRAGRELRLTIEDDGV
                     GFDPDQVPRGHLGLVGMRQRAERIGAQLKIGRRPSGGSRIRVSLPLAPDADGEVDAPA
                     ASDRSTAASVE"
BASE COUNT          730 a         1924 c         1737 g          604 t
ORIGIN      
        1 agccggccgt cccacgtctc ggcgaccacc ggcagcgacc accggggccg gtcgggtcga
       61 gcgccggcca cactggccga caccagggca tcgcgcacct cgccaaccga gtgcgtgttg
      121 gtgaggccga tgggcgtggt catcattccc gactcccgca cccactccag cccggtcatc
      181 tcgccgttcc cgttcagggt gtgggtcccg gcaaagaccg gattggcacc aatggcgtcg
      241 cgcggcagga tgacggtcac gccggtgcgc accggaccgt ggccgacccg aagcgccccg
      301 tcacctgaga tcagcgtggc atgccccacc cgcaggcccg ggacgtccgt gatggcgccc
      361 agcggcccgg cgggcagcag gccgatgcgg atcccgagct cacgggccgg catgggtgcc
      421 ctcctctgcg gtatcgaccg gcgccacctg ccccaactgc tcgtccacaa gccgctccgc
      481 cgcagcgctg ctggcggcgt ggccggtgac cgcctccagg aacacgtggc gctccagggc
      541 cagcagctcg gcgccggtca aagcacgcac ggtggccgtg cggggcacgt tgcgcagcag
      601 ggcgatctcg ccgaacgacg agccggcggc caggcgccgg acctcacggc cgtcgacggc
      661 gacgctcacc tcaccatcgg tcaggatgta gaagtgatcg ccggcctcgc cctggcggat
      721 gacgacggtc ccggcgtggg catgcaccgg cctcaggttc ccggccagcc gctccagggc
      781 cggtggcgac aacggcgcga agagcgttgt ggagcgcagg atggccagct cgcggggtcg
      841 tgccactgga accgtgtcca ggtcgcgcag cacccgccag ctgaccgcca ccagcgccag
      901 cagcccgacg ccgctggcaa gcagggcgcc gacgatgcct agcagagcga tcagcagcgg
      961 ggccagcgcc gagccgaccg ccagccccgc ggcggaaacg ccctccagca ccccgaagac
     1021 gcgggagagc agcgcgccgg gcgccacgcg ctgcagcaac gttcgtccgg agacgtcgat
     1081 caggacgcta cccactcccg cacccgcgag cagggcgaac gcgacggcga cgaccggcac
     1141 cagccccagc gcggccaggg cgatcgccca tagcgccagg ccgatggcca gcgggccgcc
     1201 gagcgccgag cgtccgatca gtcccaccgt gatcacggcg cccaccagcc cgcccaaccc
     1261 gagcgcagcg ttcaggtagc cgacgccggc ctcgccgagt gccaggcggt cgaaggccag
     1321 caccaccagg taaacgtcca gcgcgcccca cagcaccgcg gcgccggtca tcagcagcag
     1381 caccgccctg gggcgcggct cgcgggccag ggtccggaaa cccccgagcg cctccgacag
     1441 caaccctccc gcaccatcgc gcgaccgctc ggccacccac tcgtccacgt ccaggggccg
     1501 gatgccggcc accagcaggg ctccgccgcc ggtcagcgcc gccgccgcgg cgaagacggt
     1561 gccagggccg accagcacca gcaggacgcc ggccagcagc ggcccgatca gcaggctgcc
     1621 gttctcgacc agccccaccg ccacgttggc cgccgtcagc tcggacggcg cccgcgacag
     1681 cgagggaagg atcgccccct gcaccggccg ggtcagcgtg atggaggtgg cggccaacgc
     1741 cgcgaacgcg tagaccaccc agccgggact gccagcgaag agcgccacgg ccgtcatggc
     1801 catggccacc gtctgggcca ggtaggcgat cagcagggcg cgctcgcgcc gcacgtggtc
     1861 gccgagcacg gcggccaccg gcgccaccag cgccgagggg accagctgca ccacggccgc
     1921 gatccccgtc tcggtggcgc cgccgcgagc gtaggcgaag accaggatgg cgatccaggt
     1981 catccactcg gtgatggaga agccggcgaa ggcgagcagc acgcgtcgca gtgacgcgtt
     2041 acgggcaacc cccagcaccg cgccaacgcg ctgcggaacg tggacggctg gcatgccgcc
     2101 cgatcatgcc acgcgtcagc cccggcaggc ttcgatcgcg gcggccacga actcctccgg
     2161 accggccgac ttggcgacga acgcgtcgga gcgcagctgc agctcggcgg gctggttcca
     2221 acccatcagt accacgcgca cctccgggaa gtcgcggccc agcgctctca ggaaggcctc
     2281 gccggcggcg acgtccggca gccggggatc gaccaggacc acagccggcc gctgctgcgc
     2341 ggccagctgg agggcgagcg ctggatcacc ggcggtgcca accacgtcga cccccgcgat
     2401 gcgcagcacg tcggcgatcg actgctgcac gcgccggtcg gcatccacca ccgccacgcg
     2461 cggcaggcag cccggaccgg cccccgggat cgctgtggtc atgcaccgat ggtgcggccg
     2521 ggccatctgc ggcacatcgt ccctttgggg gacggccgcc tacgtcattc gaccgatgcc
     2581 gccgtgctgc ggtcggaggc agccggcgcg tccacttcgc catccgcatc gggcgcgagc
     2641 ggcaggctga cgcgaattcg cgaccctccc gatggccggc gaccgatctt cagctgcgcg
     2701 ccgatgcgct ccgcccgctg ccgcatgccc accaggccca ggtgcccgcg cgggacctgg
     2761 tccggatcga atcccacgcc gtcgtcctcg atggtcaggc gcagctcacg gccggcccgt
     2821 cgcagcccga tggtcgcgtt ctgcgcattg gcgtgcttca cgacgttgtg cagcgcctcc
     2881 tgggccacgc ggtatagggc ctcctccacg tccaaagccg tgcgctcgtc gtactcgacc
     2941 tccaccgaga cggccagtcc ggtgcgcccc tgcaccgccg caccgtggtt gcgcagcgcc
     3001 tgcgccagcc catcggtctc gagcgcctgc gggcggagct cgaagatcag cgtgcgcatc
     3061 tcggccaggg catccttctg caggtcacgc agctcccgca tcttgtcgcg agctgcggcg
     3121 ggatcgcgct ccatgaccag ctccagggcg cgcgcggtga ggcccatgct gaacagggcc
     3181 tgggtcaccg agtcgtggag ctcgcgggcc aggttcgctc gctcctcacc ggcggccagc
     3241 gccgccgcct ggcgccggag gtcctcctcc agccgctccc gctcccggac gtcgcggatg
     3301 gatccctgca ctcccgcaaa gcggccgtcg accagggtgg ccaccatgtt cacctcggcc
     3361 gggatgctgc ccccgccgga cagcgggagg acgatgcgga actggaggtc ttcctccggc
     3421 cgctcctgga ctgcccgcca cgcggcgcgc gccgcctcga gggactcggc gccgaccagg
     3481 gtctcccagt gccgccccag cagctcgctg gcgcggaagc cggtcatgcg ttcgaccgcc
     3541 tcccccaggt acgtgaagtg cccttccgcg tcgaccgacc acaccaggtc cggggagtgg
     3601 tccaccaggt ggcggtaccg ccgctccgac tcggccagct gctccaacag ccgcgcgttg
     3661 gtgatggcca ccgctgcctg ggcggccagc gcgtccagca gctgcgcgtc cgactcgctg
     3721 aaggcctcgg tccgggcggc ctgcaccgtc agcgtgccga gcggctcacc gccagccacc
     3781 agcggcgccg acatcacaga gcacatgccc cgccgccgga tgtagtcgtc cagctctggc
     3841 ccgtgcggaa aggcggggtc gttgaggtag tcgccggtgc gcaccacgcg gcgctcggac
     3901 agggacgttc cgctgacgcc ctcgcccggc gtgaccacca catcgtccag cggcacgtcg
     3961 cgcggcgcgt gggccaccgc ccatgccagg acccggctgc cgctgtccag ggggttgatg
     4021 agcgccatgt cggctcccag cagccgatgc gcctcgtcca cagtgcgctg caacacaacg
     4081 gccggatccc ggatggaagt gatctgggtg gcgatggccg atagggtgcg ttgcgcctcc
     4141 accaggtggg ccagatcctc cgccgcacgt cgcgcctcgg cgtagaggcg ggcgttcgac
     4201 tgcacgatgg cagcgtggct ggccagggcg cccagcagct ccgcgtccgg ctcgtcgaag
     4261 gcgttgggcc gcatggagta caccgtcagc gtgccgatgg ggccggagtc gccggccagc
     4321 ggcgccgaca tctgggatcg gtacccgcgg ccgcgcatcc aggcgtccgc ctcgtcgttg
     4381 tggacgaagc cccggtcgac gaggtaatcg ccggtgcggc gcacctcgcc ggactggatg
     4441 gccgccccgg ccacgccgac gcccggctcc acgtggacgt cggccaggat cggcgtgagg
     4501 tcctccgccg agagtccgcg cagcaggtcg atctccggct cgaaccggtc gatgacggcg
     4561 tggtccgcgc cgagcaggcg cttcgactcg tccaccgtgc gacgcaggac ggtgccgggg
     4621 tcgcggatgg agctgatctg ggcggcaatg gcggccatgg tgcgctgcgc ctccgccgac
     4681 cgggcaagcg catccgcccg ctcgcgcagc tgctggtaca gccgggcgtt gcgcgcggcg
     4741 atcgccccct gcccggccag ctcctcgagc aacgccacgt cccgcgggtc gatgtggcgc
     4801 ggctctcggt aagtgatcgc cagcgtgccg atcacgtcgc cgcgcacgcc gcgcaacggc
     4861 gccaccgcca ccgcgccgag ctcgagccgc gccggagcat cgtcgtccgg gtcgtgcggg
     4921 atgcgctcgt cgttggcgta gtcatcggtc caggccggct ggcccgaggc ggccgccagg
     4981 ccgttgatgc cgcca
//
LOCUS       DATACA010000268         4517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2685084, whole genome shotgun sequence.
ACCESSION   DATACA010000268 DATACA010000000
VERSION     DATACA010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2685084"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..615)
                     /locus_tag="VHK28_05505"
     CDS             complement(49..615)
                     /locus_tag="VHK28_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141718.1"
                     /translation="MSLFRTRLLALLGASLLITFSATAAFAAPPERAHGEDANHGQQV
                     SAFVHGLLFGSDESEDADEESVEEDVEEEVEEELDEEEVDGETPDNHGQCVREFAHSD
                     EVGGPNENHGFVVSEAARVTCRDTDTPEETDEQEAVEETEEVEETSTDEVDDGGGRGK
                     SAAAHERNAQRKAANAAGGRGHGRGGRH"
     gene            765..1556
                     /locus_tag="VHK28_05510"
     CDS             765..1556
                     /locus_tag="VHK28_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2141719.1"
                     /translation="MPTLTINDTPHAYQEAGQGSPVLLIHAGIADLRMWDEVWPAFTG
                     RHRSIRYDMRGYGDTPLPDGPFTYAADAAGLQQALGVERAHVVGVSMGAGVALDLALA
                     RPELVDRLVLVAPGLPDWDWAPEMDVFDEQEAELFDRGELDEASWLNVRFWLDGPTRQ
                     PEEVDADLRRRVFEMQRRAFEWENPKADGSWLVPDRGERLAEVRSSTLVVAGELDQPD
                     FAGVGQHMAERIPNARLEVMPGVAHLPPMEAPDEFSRLVLDFLRE"
     gene            complement(1768..3789)
                     /locus_tag="VHK28_05515"
     CDS             complement(1768..3789)
                     /locus_tag="VHK28_05515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX2141720.1"
                     /translation="MPASVHISGYHAAVLYDYGQVTPETVTSEADAALRRADALVEQA
                     VTAPQPTFETTLALVELAGAEISVGYGRSAFMAHVHPDAAVRDAAQAAEERLDKWRVG
                     IAFREDLFRVVRAFSQTDEAATLDSEARQLLEHWMRDFRRAGHELPPEERSELEALRN
                     RLVELQVAFSRNLNEFRDWIEVTLEELDGLPHDYVERLTPGERPGTYRVSLDYPEYNP
                     FLEQAHSRPHREALFRKDWTKAADSNRPLLAEAMTVRRRIAELLGHPTWAHYAMEVKM
                     AGRPERVRDFYEDLTPPLELAARRETEALRAMHAFNGGDGDDLQSWDWRYYDTLQARN
                     DHGVDQNVVSEYLPLDQVLDGMFALTGEVFGLEYRRVPEARAWHPSVELFEIRDRETG
                     ETLAHFYADLFPREGKYGHAAAFPIVIGHAGADGTYVKPVSAIVANFTPPSAERPSLL
                     THGPHGQIETLFHEFGHILHMSLTRARYTRFSGASTEWDFVEAPSQIMEHWVWEPSVL
                     ERFARHFRTGEVIPSDLVERMIGARYLNVGMRATRQVFFGAMDLALHATTDEPDMDAA
                     IRDAFAVTQLPYPEGTFMLAGFGHLMGGYDAGYYGYLWAEVIGDDMWSRFATEGITSP
                     EVGGAFRRAVLEPNGARPGDELVEAFLGRPASTESYLRMRGMSPEAPTR"
     gene            complement(3808..4278)
                     /locus_tag="VHK28_05520"
     CDS             complement(3808..4278)
                     /locus_tag="VHK28_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2141721.1"
                     /translation="MTERARRDRLTGLPNLAAWAEALETERMRRSRYRRPVVVMSVDV
                     DGLERINRRFGRSTGDELLVATARILRANLRDADLVARTGPDEFGVLMPETNAEAMGP
                     IVARVSEAAAAWRGTNPEARLSLSVGWAVPEPFGALGRAQHAAAEQVRLTKRGA"
BASE COUNT          648 a         1598 c         1545 g          726 t
ORIGIN      
        1 aaaaggaccc gggccgcgcg gcccgggtcc tcgcagtggg gaagcggctc agtgcctgcc
       61 gcctcgcccg tgcccgcggc cgccagccgc gttcgcggcc ttgcgctgcg cgttgcgctc
      121 gtgcgccgcg gccgacttgc ctcgtccgcc gccgtcgtcg acctcgtcgg tggaggtctc
      181 ctcgacctcc tcggtttcct cgacggcctc ctgctcgtcg gtctcctccg gggtgtcggt
      241 gtctcggcag gtcacccgag cagcctcgct gaccacgaag ccgtggttct cgttcggacc
      301 acccacctca tcactgtgcg caaactcgcg gacgcactgg ccgtggttgt ccggcgtctc
      361 gccgtcgacc tcttcctcgt cgagctcctc ctcgacttcc tcttcgacgt cctcctcgac
      421 ggactcctcg tccgcgtcct cggattcatc cgagccgaag agaaggccgt ggacgaaggc
      481 tgagacctgc tgtccatgat tggcatcctc gccgtgcgcc cgctccggtg gcgccgcgaa
      541 cgccgcggtc gccgagaacg tgatcagcag cgaagcaccc agcaatgcga gcaggcgcgt
      601 gcggaatagg ctcatgatcg atatcggcct ccaaagcatg attgcgcgga cgcatacgcg
      661 tccatgtatg acaacgccag tctcccggaa ccgatacggc ctgcggccgg tacatcggta
      721 cctgcaaccg gcggacattc ggctggacgg agcacaatgc accgatgcca accttgacga
      781 tcaacgacac cccgcacgcc taccaggagg cggggcaagg gagcccggtg ctcctgatcc
      841 acgccgggat cgccgacctg cggatgtggg atgaggtgtg gcccgcgttc accgggcgac
      901 atcggtccat ccggtacgac atgcggggct acggcgacac gccgctcccg gacgggccgt
      961 tcacctacgc ggccgacgcc gcggggctgc agcaggcgct gggcgtcgag cgcgcacacg
     1021 tggtgggcgt gtccatgggc gccggggtgg cgctcgacct cgccctggcg cgtcccgagc
     1081 tggtggatcg gctggtgctg gtggcgccgg gcctcccgga ctgggactgg gctccggaga
     1141 tggatgtgtt cgacgagcag gaggcggagc tgttcgaccg cggggagctg gacgaggcgt
     1201 cctggctcaa cgtgcgcttc tggctggacg gccccacccg ccagccggag gaggtggacg
     1261 ccgaccttcg ccggcgggtg ttcgagatgc agcgtcgcgc cttcgagtgg gagaacccga
     1321 aggccgacgg ctcatggctg gtccccgacc gcggtgagcg cctcgccgag gtgcggtcat
     1381 ccacgctggt cgtggccggc gagctggacc agccggattt cgccggcgtc gggcagcaca
     1441 tggccgagcg catcccgaac gcgcggctgg aggtcatgcc cggcgtcgcg cacctgccgc
     1501 ccatggaggc gccggacgag ttcagccggc tggtgctcga cttcctcagg gagtgaccgt
     1561 tcggtggcac cgcccaaata tcgggcagta gttcacgcgg ccgcctcgct gcgtgtcgtt
     1621 ctgccgatac aacgggcagc tgtgagcatg ggggacgaag aacttgtcgg ttcctgcgtg
     1681 ggcctgcccg acagatcggc agatcgacac ctggccaacc gctcgtgtga agaaccgccc
     1741 gatatatcag gtcggcgagg tcggccccta gcgcgtggga gcctccggac tcatgccccg
     1801 catgcgcagg tagctctccg tggaagccgg ccggccgaga aatgcctcca ccagctcgtc
     1861 tccgggccgc gccccgttcg gctccagcac ggcgcgacgg aacgcacctc ccacctccgg
     1921 tgaggtgatc ccctcggtcg cgaagcggct ccacatgtcg tctccgatga cctccgccca
     1981 caggtatccg tagtagccgg cgtcgtagcc acccatcagg tgcccgaagc cggccagcat
     2041 gaaggtgccc tccgggtagg ggagctgggt gaccgcgaag gcgtcgcgga tggcggcgtc
     2101 catatcgggc tcatcggtcg tggcgtgcag ggcaaggtcc atggccccga agaagacctg
     2161 tcgggtggcg cgcatcccca cgttcaggta tcgggcgccg atcatgcgct ccaccaggtc
     2221 gctgggaatg acctcgccgg tgcggaaatg ccgcgcgaac cgctccagga ccgatggctc
     2281 ccagacccag tgctccatga tctgagacgg cgcctccacg aagtcccact cggtcgaggc
     2341 cccgctgaag cgggtgtacc gggcgcgggt gaggctcatg tgcaggatgt ggccgaactc
     2401 gtggaagagc gtctcgatct gcccgtgcgg gccgtgggtc agcaggctgg gccgctccgc
     2461 ggatggcggg gtgaagttgg ccacgatggc gctaacgggc ttgacgtacg tcccgtccgc
     2521 cccggcatga ccgatgacga tcgggaaggc ggcggcatgc ccgtatttgc cctccctcgg
     2581 gaacaggtcg gcatagaagt gagccagcgt ctcgcccgtt tcgcggtcgc ggatctcgaa
     2641 caactccacc gacgggtgcc aggcgcgcgc ctccggcacg cggcggtact ccaggccgaa
     2701 cacctcgccg gtcagcgcga acatcccgtc caggacctgg tccagcggta gatactcgct
     2761 gaccacgttc tggtcgacgc catggtcgtt ccgcgcctgc agcgtgtcgt agtagcgcca
     2821 gtcccaggat tgcaggtcgt ccccatcgcc gccgttgaac gcgtgcatgg cacgcagcgc
     2881 ctcggtctcg cgccgtgccg cgagctccag cgggggagtg aggtcctcgt agaagtcccg
     2941 cacccgttcc gggcggcccg ccatcttgac ctccatggcg taatgcgccc aggtcgggtg
     3001 ccccagcagt tcggcgatgc gtcgacgcac cgtcatggcc tcggccagca gcggccggtt
     3061 ggagtccgcg gccttcgtcc agtccttgcg gaagagcgcc tcgcggtgcg ggcggctgtg
     3121 cgcctgctcc aggaacgggt tgtactcggg gtagtccagg ctgacgcggt acgtccctgg
     3181 ccgctcgccc ggcgtcaggc gctcgacgta gtcgtgtggc aggccgtcca gctcctcgag
     3241 cgtcacctcg atccagtcgc ggaactcgtt caggttgcgg ctgaaggcca cctgcagctc
     3301 caccaaccgg ttgcgcaacg cctcgagctc ggaccgctcc tccggcggca gctcatggcc
     3361 cgcccgacgg aaatcgcgca tccagtgctc cagcagctgc cgggcctcgc tgtcgagcgt
     3421 ggccgcttca tcggtctggc tgaaggctcg aacgacccgg aacaggtcct cccggaaggc
     3481 gatgccgact cgccacttgt ccaggcgctc ctcggccgcc tgcgccgcgt cgcggactgc
     3541 ggcatcggga tggacatgcg ccatgaacgc gctccgccca tacccgaccg atatctccgc
     3601 ccccgccagc tccaccagcg ccagggttgt ttcgaaggtg ggctgggggg ccgtgaccgc
     3661 ctgctcgacc agcgcatcgg ctcggcgcag ggcagcatcg gcctcgctgg tgaccgtttc
     3721 gggcgtcacc tgcccgtagt cgtagagcac ggcggcatgg tagccgctga tgtggaccga
     3781 tgccggcatg gccaccggcc cttcccgtca ggcgccgcgc ttggtcaggc gaacctgctc
     3841 agccgccgcg tgctgtgccc gtccgagcgc gccgaacggc tccgggaccg cccaaccgac
     3901 cgagagcgac aggcgtgcct ccgggttagt gccgcgccag gccgcagctg cctcgctcac
     3961 ccgcgcgacg atcggcccca tggcctctgc gttggtctcg ggcatcagca cgccgaactc
     4021 atccggcccg gtgcgtgcca ccaggtccgc gtcgcgcagg ttggcgcgca ggatccgcgc
     4081 cgtggccacc agcagctcgt caccggtgct gcgcccgaag cggcggttga tccgctcgag
     4141 cccgtccacg tccacgctca ttaccaccac cgggcggcgg tagcgcgaac gtcgcatgcg
     4201 ctccgtctcg agtgcctcgg cccatgccgc gaggttcggc agcccggtca ggcggtcccg
     4261 ccgggcgcgt tcggtcatga gcacagtgtg agggcgcgtg cggcccatcc ggaatggccc
     4321 gataggacca taagccgcgt tggagtgggg ttcgccaggt gcgtccttca ggccgcccga
     4381 gacgccgggc ctctccctcc ccagccggcc cactggtcgc cgatgcgcgc gttgcgcagg
     4441 aggtgctcgc cgtagagcac ggcctggtcg acggtgagcg tgctgcgcac gtcgtagtcg
     4501 ctacgcaacg ggtagca
//
LOCUS       DATACA010000269         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_838067, whole genome shotgun sequence.
ACCESSION   DATACA010000269 DATACA010000000
VERSION     DATACA010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838067"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VHK28_05525"
     CDS             complement(<1..182)
                     /locus_tag="VHK28_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141722.1"
                     /translation="MHPGRAPADKPAPVTIGGSPRTEPRLLAARLALATGGWMKLQEY
                     RSKEVLARHGVPLLDG"
     gene            231..1244
                     /locus_tag="VHK28_05530"
     CDS             231..1244
                     /locus_tag="VHK28_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX2141723.1"
                     /translation="MSYRVTLIAGDGIGPELAEATVGVLEATGIGFEWDVQAAGEETI
                     VSEGTPLPDRVVESIRRNGIAIKGPITTPVGSGFRSVNVGLRQALELFANVRPARSMK
                     GVETRYDDVDLVIVRENTEDLYAGIEHRVGPDAAESIKIITRAASQRIARYAFEYAVR
                     NGRRKVTAVHKANIMKLSDGLFLESCQQVAAEYAGRVEFEDRIVDNMCMQLVQKPDLY
                     DVLVLPNLYGDIVSDLAAGLVGGLGVAPGANIGEAAAVFEPVHGSAPKYAGQNRANPT
                     ALILSAALMLRHLGETDAGDRVETAVREVIAEGRTVTHDLGGTAGTLEFGQAVAERVR
                     AST"
     gene            1692..2069
                     /gene="sdhC"
                     /locus_tag="VHK28_05535"
     CDS             1692..2069
                     /gene="sdhC"
                     /locus_tag="VHK28_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX2141724.1"
                     /translation="MVRLYRYQVSWAELAWIGHRLSGIGVLVFLFLHVVETATVLFGP
                     EVYDFTQTFYHNLPAKLGEVLLMAALVYHALNGIRVIVMDFWPRLTLYYRPLTYGVVV
                     ATVVTMIPVGLIMIRDYAPWGGG"
     gene            2069..2527
                     /locus_tag="VHK28_05540"
     CDS             2069..2527
                     /locus_tag="VHK28_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX2141725.1"
                     /translation="MSVGRRVWPVQGDPLRRNPSRAAIPRPEEPGRDRLWWYFMRISG
                     LLLVLLALGHMFIMHVMVELTGGEINFAFVTSRWGTPFWRIYDGALLLLAMVHGVRGA
                     RIVITDYTHGGFRTFLVWLLVAVTAVWLVLGLAVIVLFDPNAGASVGPFS"
     gene            2524..>4180
                     /gene="sdhA"
                     /locus_tag="VHK28_05545"
     CDS             2524..>4180
                     /gene="sdhA"
                     /locus_tag="VHK28_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019932953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX2141726.1"
                     /translation="MSLVQTDAAAARPFVRTHELDAVVVGAGGAGLYAALELKQELGP
                     EARIGVISKLYPSRSHTGAAQGGVCAALANTEEDHWEWHWFDTVKGGDYLVDQDAAEV
                     MCRDAIETIINLEHLGLPFDRTPEGLINQRRFGGHTRNYGEAPVRRSCYAADRTGHAI
                     LQTLFQQCIRADVAFYDEYQVLDLAMPDGPDGRVTGVIGLQLSTGEIHSFRARGVMFA
                     TGGFGKMYKVTSNAHTLTGDGVAITWRRGLPLEDMEFFQFHPTGMYKLGFLLSEAMRG
                     EGGILLNGEGERFMERYAPTMKDLASRDVVSRSIYQEIAAGRGIDGQDYVHLDVRHLG
                     SEVLNTKLPDMSGFIRTYFGLDPVTELVPIQPTAHYAMGGIPTDVDGRVLADESGRTV
                     PGFYAAGECACVSVHGANRLGTNSLLDILVFGRRAGKAMADELASAPDAVPDTEAEVA
                     VSRAMSGLLGRSEGERAATLRLELQEVMFNKCGVYRTAKLLEECQAEVQALRERARHL
                     VIDDKGCRYNTDLGEALELGFLIDCAQATVDSAVARLESRGAHA"
BASE COUNT          632 a         1426 c         1465 g          657 t
ORIGIN      
        1 tcaccgtcca ggagcgggac gccgtggcgc gccaggacct ccttggaccg atactcctgg
       61 agcttcatcc aacctcctgt cgcaagagcg agccgcgcag ccagaaggcg cggctcggtg
      121 cgggggctgc caccgattgt aaccggagcc ggcttgtcag ccggcgctcg ccccgggtgc
      181 atgccgttct gacgcctaac gcaacctgcc tgcccgccta cactccctcc gtgagctatc
      241 gagtcacctt gattgccggc gacggcatcg gtccggagct ggcggaggcc accgtcggcg
      301 tcctggaggc caccggcatc ggcttcgaat gggacgtgca ggctgccggc gaggagacga
      361 tcgtttcgga gggcacgccc ttgccggacc gggtggtgga atccatccgg cgcaacggga
      421 tcgccatcaa ggggccgatc accaccccgg tggggagcgg cttccgcagc gtgaacgtcg
      481 ggctgcggca ggcgctggag ctgttcgcca acgtccgacc ggcgcgcagc atgaagggcg
      541 tcgagacccg ctacgacgac gtggacctgg tgatcgtccg cgagaacacc gaggacctgt
      601 acgccggcat cgagcaccgg gtggggccgg acgccgccga atcgatcaag atcatcaccc
      661 gcgctgcctc gcagcgcatc gcgcgctacg ccttcgagta cgcggtgcgc aacggccgtc
      721 gcaaggtgac cgccgtgcac aaggccaaca tcatgaagct ctccgacggc ctcttcctgg
      781 agagctgcca gcaggtcgcc gccgagtacg cggggcgcgt ggagttcgag gaccgcatcg
      841 tcgacaatat gtgcatgcag ctggtccaga agcccgacct ctacgacgtg ctggtgctgc
      901 cgaacctgta cggcgacatc gtgtccgacc tggcggcggg cctggtcggc gggctgggcg
      961 ttgcgccggg ggccaacatc ggggaggcgg cggccgtctt cgagcccgtc cacgggtcgg
     1021 cgccgaagta cgcgggccag aaccgggcga accccaccgc cctcatcctg tcggcggccc
     1081 tgatgctccg ccaccttggc gagaccgatg ccggcgaccg ggtcgagacc gcagttcgcg
     1141 aggtgatcgc cgagggtcgg accgtcaccc acgacctggg cggcacggcc gggaccctcg
     1201 agttcggcca ggcggtggcc gagcgagtcc gcgcctcgac ctgaggggct gcggcgcgga
     1261 gttccgactt gtcgccagat tcagacgagg cgcccatcgg ctgagctgcg gcgcctcccc
     1321 gccgccccgt gccggctcat gcgcgcaccg tggcggccaa ggtcggggtt aaaccacgtc
     1381 gtgcgcactc cgtatgcatc tggcgagccc gccgcgctcc tggcgcgaag caggcgaatt
     1441 cacatcacat gaccggttgg cgggaaatct cgccgccgga gcgtctgggt tcacgcctgg
     1501 cgtgaatccg gagcattcaa cagcccgaca cgacaccgga cgcgctccgg cgtgaattgg
     1561 ggccgttcgc ggatcccctc ggtccgccgg cggccggacc catccccgag cgccgcgcta
     1621 gaatcgaggg gccgctgctg cacgacgcat cgtgccgtgg cgcccgcccc gagagggaag
     1681 cgaggagcag attggtcagg ctgtacaggt atcaggtcag ctgggccgag ctggcatgga
     1741 tcggccaccg gctctcgggc atcggtgtgc tggtcttcct gttcctgcac gtggtcgaga
     1801 cggccaccgt gttgttcggg ccggaggtct acgacttcac ccagaccttc taccacaacc
     1861 tccccgccaa gctgggcgag gtgctgctga tggcggccct ggtctaccac gcgctcaacg
     1921 gcatccgggt catcgtcatg gacttctggc cgcgactgac gctgtactac cgcccgctta
     1981 cctacggcgt ggtggtggcc accgtggtga ccatgatccc ggtggggctg atcatgatcc
     2041 gcgactacgc cccgtggggt ggcggctgat gtcggtcgga cgtcgggtgt ggccggttca
     2101 gggcgacccg ctgcgacgca atccgtcgcg ggccgccatc ccgcgcccgg aggagcccgg
     2161 ccgcgatcgg ctgtggtggt acttcatgcg catcagcggg ctgctgctgg tgctgctggc
     2221 gctgggccac atgttcatca tgcacgtgat ggtcgagctg accggcggcg agatcaactt
     2281 cgccttcgtc accagccgct ggggcacgcc gttctggcgc atctacgacg gcgcgctcct
     2341 gctgctggcc atggtgcatg gcgtgcgcgg ggcccgcatc gtcatcaccg actacacgca
     2401 cggcggcttc cgcaccttcc tggtctggct gctggtggcc gtcaccgccg tgtggctggt
     2461 gctggggctg gccgtgatcg tcctcttcga cccgaacgcc ggagcgtcgg tggggccgtt
     2521 ctcgtgagcc tggtccagac cgatgcggcc gctgcgcggc cgttcgtccg cacccacgag
     2581 ctggacgccg tggtggtcgg cgccggcggc gccggcctgt acgccgcgct ggagctgaag
     2641 caggagctcg ggccggaggc ccgcatcggt gtcatcagca agctgtaccc ctcgcgctcg
     2701 cacacgggtg cggcgcaggg aggcgtgtgc gcggccctcg ccaacacgga ggaggaccac
     2761 tgggagtggc actggttcga cacggtcaag ggcggggact acctggtgga ccaggacgcg
     2821 gccgaggtca tgtgccgaga tgccatcgag accatcatca acctggagca cctgggcctg
     2881 ccgttcgacc gcacccccga ggggctgatc aaccagcgac gcttcggcgg ccacacccgt
     2941 aactacggcg aggcaccggt ccgccgcagc tgctacgcgg ccgaccgcac gggccacgcc
     3001 atccttcaga cgctcttcca gcagtgcatc cgggctgacg tcgccttcta cgacgagtac
     3061 caggtgctcg acctggccat gccggacggg cccgatggtc gggtgaccgg cgtcattggc
     3121 ctccagctgt cgacaggcga gatccactcc ttccgggcgc gcggcgtcat gttcgccacc
     3181 ggtggcttcg ggaagatgta caaggtcacc tccaacgccc atacgctgac cggcgacggc
     3241 gtggccatca cctggcgtcg gggcctgccg ctggaggaca tggagttctt ccagttccat
     3301 cccaccggca tgtacaagct cggcttcctg ctctccgagg cgatgcgtgg cgagggcggc
     3361 atcctgctca acggcgaggg ggagcgcttc atggagcgct acgcgccgac catgaaggac
     3421 ctggccagcc gggacgtggt cagccgcagc atctaccagg agatcgccgc cgggcgcggg
     3481 atcgatggcc aggactacgt tcacctcgac gtgcgccacc ttggctccga ggtgctgaac
     3541 accaagcttc cggacatgag cggattcatc cgcacctact tcggcctcga tccggtgacc
     3601 gagctggtgc ccatccagcc cactgcgcac tacgccatgg gcggcatccc gaccgacgtc
     3661 gacggccgcg tgctggccga cgagagcggg cgcacggtgc ccggcttcta cgcggcgggt
     3721 gagtgtgcct gcgtatcggt ccacggtgcg aaccggctgg ggaccaactc attgctggac
     3781 atcctggtct tcgggcgtcg cgccggaaag gcgatggccg atgagctggc gtcggcgccg
     3841 gatgcagtgc cggacacgga ggcggaggtg gccgtctcgc gcgccatgag tgggctgctg
     3901 ggcaggagtg agggggagcg ggcggcgacc cttcgcctcg agctgcagga ggtaatgttc
     3961 aacaagtgcg gcgtgtaccg cacggccaag ctgctggagg agtgccaggc cgaggtgcag
     4021 gccctacgcg agcgagctcg ccacctggtc atcgacgaca agggctgtcg ctacaacacg
     4081 gacctcggtg aggcgctgga gctcggcttc ctgatcgact gcgcgcaggc caccgtcgac
     4141 tcggccgtgg cccgcctgga gagccgcggc gcccacgccc
//
LOCUS       DATACA010000270         4979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2846623, whole genome shotgun sequence.
ACCESSION   DATACA010000270 DATACA010000000
VERSION     DATACA010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846623"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VHK28_05550"
     CDS             complement(<1..260)
                     /locus_tag="VHK28_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141727.1"
                     /translation="MYTPIEASVPGKVSAMRYRYGRFTTNLVFNGAIGGKCSTGTALR
                     GRMRRSAPGTDGIVAGRRYRCGISQRSRAPMVPSSPPCITGAA"
     gene            322..858
                     /locus_tag="VHK28_05555"
     CDS             322..858
                     /locus_tag="VHK28_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX2141728.1"
                     /translation="MTALVLGASAGIATPTAQAATEHRIDISGFAFNPSTLTIQVGDT
                     VTWTNADDAPHTATADGGAFDSGNLDTGASFSHTFTQAGTFSYECEYHSEMEATIVVV
                     PAASQAPAAAATSPSAAAAATAAATAGQPDTAVPAPPPGGMRPAIVVMGLGLLLMGLA
                     IVPLGPRPGAVVRSGWRR"
     gene            complement(868..1233)
                     /locus_tag="VHK28_05560"
     CDS             complement(868..1233)
                     /locus_tag="VHK28_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2141729.1"
                     /translation="MRKMRGVGQLALRTTDPSRVAEFYASAFGFKRRAEDASGVDLEL
                     GGFELSFRRGEQATRDGIQIGFRVDTAAEVDGWAQDLARRGFALVAPPSGDRWEGMRS
                     FRVADPDGRQVAVFYVYPG"
     gene            complement(1237..3054)
                     /gene="thrS"
                     /locus_tag="VHK28_05565"
     CDS             complement(1237..3054)
                     /gene="thrS"
                     /locus_tag="VHK28_05565"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2141730.1"
                     /translation="MAIDSEEVRTEADAMLDAGAHDRLDTMRHSAAHVMAAAVLDMFP
                     GARLGIGPAIRDGFYYDFDLPRPLTPDDLAEIEERMRAQVAASLVFEREELDRDTALA
                     RLEEQGQDYKVEIVRELPPEQSEVVSFYQHGAFIDLCRGPHVESTARLGPFKLLNSAG
                     AYWRGDEKRPMLQRIYGTVWESQEDLDRYLWRMEEARKRDHRRLGRDLDLFTFHPESP
                     AAPFWHPRGMALYRALEEWSRQVRREGGFHEVRTPSLVRKELWEQSGHWELYQDNMFV
                     LDDYEHTSGLKPMNCPQEILIYKTRLRSYRDLPLRFADYSVLMRKEVTGALSGMFRVR
                     QLVQDDSHVFCREDQIPEEVALALRLVRRQYEPFGFEPRFKLATRPEKRLGSDEFWDM
                     AEGKLRDALVANELAFEVDPGGAAFYAPKIDVFIEDALGREWQMATVQLDYQLPQRFD
                     LEYRAADGGFERPVIIHYAIYGSFERFTGIITEHYAGAFPLWLAPVQVMVVPIADRHI
                     EYAASVRDALAAAGLRAEVDDRSERMQAKLRDAQEQKMPVMVVVGDRDQEAGAVSPRL
                     RTGESSQGVALDDFVADLVGRVERRELWPYAEEAAEAAS"
     gene            complement(3087..3620)
                     /gene="paaD"
                     /locus_tag="VHK28_05570"
     CDS             complement(3087..3620)
                     /gene="paaD"
                     /locus_tag="VHK28_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX2141731.1"
                     /translation="MTEPATLRDPREVVVWSALDEIADPEIPAVSVVELGVIGSVRFV
                     ARPDGGERLSVELLPTFVGCPAIDVMQQQIGERLRAMQVADEVDVEVSFAVPWTSDRI
                     TPEGREKLRGSGFAPPVLIGPTFSGEELQLMLPVAECPYCGSRNTALENAFGPTLCRA
                     IYHCANCRQPFEQFKAV"
     gene            complement(3617..4366)
                     /gene="paaC"
                     /locus_tag="VHK28_05575"
     CDS             complement(3617..4366)
                     /gene="paaC"
                     /locus_tag="VHK28_05575"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX2141732.1"
                     /translation="MTEPLAELLLTLADDEFVLGFWDSEWTGIAPMLEEDVAMSSVSQ
                     DEIGHAKAWYEQLASLTDDDADRLAFGRAPDEYRHAALMNHARTDWGFTVARRFLYEH
                     ADAVRLEALSRSSHAPMAELASKMRREETYHLMHFDLWTRRLADAGGEARERLTTALE
                     RLWTDAAAVFTPLAAEQQLLEAGHLPEPMATLCARWRDRVGGLLAERGLPQPPADLQP
                     PADGRTRRTDEFSWLHGEFTMVARSEVGATW"
     gene            complement(4451..4777)
                     /locus_tag="VHK28_05580"
     CDS             complement(4451..4777)
                     /locus_tag="VHK28_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HEX2141733.1"
                     /translation="MNAPQDRVWEVFRQEADGDPMVHGGSVRAPDEELALHYAREFYG
                     RRQESHRLWVVPREAIVELDDPDLLKPPFDRSFKRPGGYLIKHKLEAAKARAGAGRTT
                     EAPTDG"
BASE COUNT          754 a         1784 c         1695 g          746 t
ORIGIN      
        1 gcggcgccgg tgatgcatgg cggcgacgaa ggcaccatcg gcgcgcggga tcgttgggaa
       61 atgccgcacc ggtaccgcct gccggcgacg atgccgtcgg ttccgggggc cgatcggcgc
      121 atacgtcctc gcaaggccgt acccgtactg catttcccac cgatagcgcc gttgaacacc
      181 agattcgtcg taaagcggcc atatcggtag cgcatcgctg aaacctttcc cggcaccgat
      241 gcctcgatag gggtgtacac gccgcattgg ggatttgctt tgctccgtcc agccttccgc
      301 ctcctgggtc tcgtcgccgg catgacggcc ctcgtccttg gtgccagcgc cgggatcgcc
      361 acgccgacag ctcaggccgc caccgagcac cgcatcgaca tcagcggatt cgccttcaac
      421 ccgtccacgc tgaccatcca ggttggcgac accgtcacct ggaccaacgc cgacgatgcc
      481 ccgcacaccg cgacggccga cggtggggcc ttcgattcgg gcaacctgga cactggcgcc
      541 tcgttcagcc acaccttcac tcaggcgggc accttcagct atgagtgcga gtaccacagc
      601 gagatggagg cgaccatcgt ggtggtgccc gcagcatccc aggcaccggc cgccgccgcg
      661 acgtcgccct ccgcggcagc cgccgcaacg gccgcagcca ccgccgggca gccggatacc
      721 gccgtacccg ccccgccgcc cggcggcatg cgtccggcca tcgtggtgat gggcctcggc
      781 ctgctgctca tgggcctggc catcgtcccg ctcgggccgc gccccggcgc cgtggtcaga
      841 tccggctggc gccgctaggc accgccctca gccggggtag acgtagaaaa cggccacctg
      901 ccggccgtcg gggtcggcca cgcgaaagga gcgcatcccc tcccatcggt ctccggaagg
      961 aggggcgacc agggcgaacc cgcgccgggc caggtcctgg gcccagccgt ccacctcggc
     1021 agcggtgtcg acccggaagc cgatctggat gccgtcgcgg gtggcctgtt cgccgcgtcg
     1081 aaagctcagc tcgaagccgc ccagctccag gtccacgccc gaggcgtcct ccgcgcgccg
     1141 cttgaagcca aaggcggagg cgtaaaactc ggcaactcgg gagggatccg tggtgcgcag
     1201 ggccagctgc cccacgccgc gcatcttccg catgcgctac gacgcggcct cggccgcctc
     1261 ctcggcgtac ggccacagct cgcggcgctc gacccgaccg accaggtcgg ccacgaagtc
     1321 gtcgagcgcc acgccctgcg aggactcccc ggtgcgcagc cgcggcgaga cggcacctgc
     1381 ctcctggtca cggtcgccga ccacgaccat gaccggcatc ttctgctcct gggcatcgcg
     1441 cagcttggcc tgcatgcgtt cgctgcgatc gtccacctcg gcgcgcagac cggcggccgc
     1501 cagtgcgtcg cggacgctgg cggcgtactc gatgtggcga tcggcgattg gcaccaccat
     1561 cacctgcacc ggcgccagcc acagcgggaa ggcgccggcg tagtgctcgg tgatgatgcc
     1621 ggtgaagcgc tcgaacgagc cgtagatggc gtagtggatg atcaccggcc gctcgaagcc
     1681 gccatctgcc gcgcggtact cgaggtcgaa ccgctgaggg agctggtagt cgagctggac
     1741 ggtggccatc tgccactccc tgcccagcgc atcctcgatg aacacgtcga tcttcggcgc
     1801 gtagaaggct gctccgcccg ggtcgacctc gaaggcgagc tcgttggcga ccagggcgtc
     1861 acgcagcttg ccctcggcca tgtcccagaa ctcatccgac cccagccgct tctcgggtcg
     1921 agtggcaagc ttgaagcgcg gctcgaaccc gaacggctcg tactggcgac gcaccagccg
     1981 cagcgcaagc gcgacctcct cggggatctg gtcctcgcgg cagaagacgt gcgagtcgtc
     2041 ctggaccagc tggcggacgc ggaacatgcc cgacagggcg ccggtgacct ccttgcgcat
     2101 cagcaccgag tagtcggcga agcgcagcgg caggtcgcgg taggagcgca ggcgcgtctt
     2161 gtagatcagt atctcctgcg ggcagttcat gggcttgagt cccgaggtgt gttcgtagtc
     2221 gtccagcacg aacatgttgt cctggtacag ctcccagtga ccggactgct cccacagctc
     2281 cttgcgcacc aggctcggtg tacggacctc gtggaagcca ccctcgcggc gcacctggcg
     2341 tgaccactcc tccagcgccc gatacagcgc catgccccgc ggatgccaga acggcgcggc
     2401 cggcgactcg gggtggaagg tgaagaggtc gaggtcgcga ccgaggcgcc ggtggtcgcg
     2461 cttgcgcgcc tcctccatgc gccacaggta tcggtccagg tcctcctggg actcccacac
     2521 ggtgccgtag atgcgctgca gcatcggccg cttctcgtcg ccacgccagt aggcaccggc
     2581 cgagttcagg agcttgaagg ggccgagtcg tgcggtggat tccacatgcg gcccgcggca
     2641 caggtcgatg aacgcgccgt gctggtagaa gctgacgacc tccgactgct cgggcggcag
     2701 ctcacgaacg atctcgacct tgtagtcctg gccctgctcc tccagccggg cgagcgccgt
     2761 gtcgcggtcg agctcctcgc gctcgaagac caggctggcc gcgacctggg cgcgcatccg
     2821 ctcctcgatc tcggccaggt cgtccggggt cagcgggcgc ggcaggtcga agtcgtagta
     2881 gaagccgtca cggatggctg gaccgatgcc gaggcgcgct cccggaaaca tgtccaggac
     2941 cgccgcggcc atgacgtggg ccgccgaatg gcgcatggtg tccagccggt cgtgggcgcc
     3001 cgcgtcgagc atggcgtccg cctcggtgcg gacctcttcg gagtcaatgg ccatgcggcg
     3061 aagggtagcg cacgcgtcgg cggcggtcag acggccttga actgctcgaa cggctgccgg
     3121 cagttggcgc agtggtagat ggcgcggcac aaggtgggcc cgaacgcgtt ctccagggcg
     3181 gtgttgcgcg agccgcagta cgggcactcc gcgaccggca gcatcagctg cagctcctcc
     3241 ccgctgaagg ttggaccgat gaggaccggc ggcgcgaacc cgctcccccg cagcttctcg
     3301 cggccctcgg gggtaatgcg atcggatgtc cacggcacgg cgaaggagac ctcgacgtcg
     3361 acctcgtccg ccacctgcat ggcgcgcagc cgctccccga tctgctgctg catgacgtcg
     3421 atggccgggc agccgacgaa ggtgggcagc agctcgacgc tcagacgctc cccgccatcc
     3481 ggacgggcca cgaagcgcac gctgccgatg acccccagct ccacgacgct gacggccggg
     3541 atctccgggt ccgcgatctc gtcgagggcc gaccagacca ctacctcccg cgggtcgcgc
     3601 agcgtcgccg gctcggtcac cacgtcgccc ccacctccga gcgcgccacc atcgtgaact
     3661 cgccgtggag ccagctgaac tcatcggtac gccgcgtccg gccgtcggcc ggcggctgca
     3721 ggtcagccgg cggctgcggc agcccgcgtt cggcgagcag gccgcccacg cggtcgcgcc
     3781 atcgggcgca gagcgtggcc atcggttccg ggaggtgacc cgcctcgagc agctgctgct
     3841 ctgcagccag cggggtgaag acggcggccg catcggtcca caggcgctca agagcggtgg
     3901 tgaggcgctc ccgagcctcg ccgcccgcat ccgccaggcg tcgcgtccac aggtcgaagt
     3961 gcatgaggtg gtacgtctcc tcgcgccgca tcttggatgc cagctcggcc atgggtgcgt
     4021 gcgacgagcg tgagagcgcc tccaggcgta ccgcgtccgc atgctcgtac aggaagcgac
     4081 gcgccacggt gaagccccaa tcggtgcgcg cgtggttcat gagcgccgcg tggcggtact
     4141 cgtcgggtgc gcgtccgaag gccaggcgat cggcgtcgtc atcggtcagg gaggcgagct
     4201 gctcgtacca ggccttggca tgcccgatct cgtcctggct gaccgagctc atggcgacgt
     4261 cctcctccag catcggcgcg atgccggtcc actccgagtc ccagaagccg agcacgaact
     4321 cgtcgtcggc cagggtgagc agcagctcgg cgagcggctc ggtcatggca ggcggtccgg
     4381 gcgcctgtca tggccggttg tgcggctgtg ctgctgtgcg cctgtgcggc acaacggcgc
     4441 ctgacgcgcg tcatccatcc gtcggagcct cagtcgtgcg ccctgcgccg gcgcgggcct
     4501 tggcggcctc gagcttgtgc ttgatgaggt agccgcccgg gcgcttgaag gaccgatcga
     4561 agggcggctt cagcaggtcc gggtcgtcca gctccacgat cgcctcgcgc ggaaccaccc
     4621 acagccgatg gctctcctgg cgccggccgt agaactcccg ggcgtagtgc agggccagct
     4681 cctcgtcggg cgcgcggacg ctgcctccgt gcaccatcgg gtcgccatcc gcctcctggc
     4741 gaaagacctc ccagacccga tcctgcggcg cgttcatgcg ggcaagcgta gcgcgccccc
     4801 ggggtcaccg ccatgagccg gcaagcactc gccgccccga cggtcagtcg aggagaagtg
     4861 gcacgcgcgt tgcattcgta caccggcacg cagggggatg cgatgtcacc gagtactgaa
     4921 ccgacaagaa tcgcggtctt ccgcgcgctg cagctgggag acatgctgtg tgccgttcc
//
LOCUS       DATACA010000271         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1561547, whole genome shotgun sequence.
ACCESSION   DATACA010000271 DATACA010000000
VERSION     DATACA010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561547"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VHK28_05585"
     CDS             <1..373
                     /locus_tag="VHK28_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX2141734.1"
                     /translation="SAPRGRAGSVAPATVSGSYRRADGTVSPTGRRRSRHPLLVVAQA
                     KAKADALAGLDRWKARHPEAAAHLAEDDVLVDGMRGRSSLWYRVRVNLRHVPEDARPD
                     QEPPDPDYDPADEWLGAESRD"
     gene            complement(383..964)
                     /locus_tag="VHK28_05590"
     CDS             complement(383..964)
                     /locus_tag="VHK28_05590"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX2141735.1"
                     /translation="MRAPERVTLAGRHVRLEPIEERHRDDLLAAAAQDPETFRYTLSQ
                     LWRGAAEWPAYLADALRPDFITWATVDTVSGRAIGSTRFGDISPADGRLEIGWTWIAP
                     SHQRTAVNTEAKLLQLTYAFEQLGATRVALKTDARNERSQRAIERLGGVREGVLRRHL
                     RLPDGFIRDTVYYSILDDEWPSVKERLTARLNR"
     gene            complement(1112..2818)
                     /locus_tag="VHK28_05595"
     CDS             complement(1112..2818)
                     /locus_tag="VHK28_05595"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2141736.1"
                     /translation="MSAQPANPDPNAAVNELADRFFEGVLEREPIFATILGDDRYDDR
                     LPDLGAQGRADEAAAYRGVLAEAEAIDPAALGVEQVITRDMLMLVARNQLEALDRKLY
                     QLAVNHISGVQLMPVTVAQYQIAESPDGLERLLARFRAYPTAVMQNIETLREGVADGR
                     TAARIPVDKQVQQIERLVATPTEQFPAVTLAHVADDDARNAVAAAVDEHVKPALQQLR
                     DYLADDYAAHARTDPGLSSTPDGAEAYRLSIRLQTTVATSAEEVHAFGLEDLARIEAE
                     MDEIARRLGHPDRHALKAALETDPTNHTDSRDAIVQLAVEQTERAYAAAPRYFGRLPS
                     ANCVVKPVEEYRERETPPAFYMQPSIDGKRQGQYYINTYQPEERQLHKIAAITFHEAT
                     PGHHFQIGIEMELTGLPRFRTLGGRMAGVAYVEGWGLYTERLADEMGLYLDDRERLGM
                     LDAQAFRAARLVVDSGLHAMGWSRNKAIEFMHERGTLPDVDAEIEVDRYTVWPAQALA
                     YKIGQREIERARREVSEKLGDRFDLRAFHDEVLGHGSLPLATLRREIAGWVEAAVVSP
                     AR"
     gene            complement(2916..>3917)
                     /locus_tag="VHK28_05600"
     CDS             complement(2916..>3917)
                     /locus_tag="VHK28_05600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2141737.1"
                     /translation="EPKWDGFRAIVFRDGQEVYLQSRDLKPLDRYFPELADPVRAIGT
                     ADACFVLDGEIVIARDGGLDFDALLLRIHPAASRVAMLAGETPASFVAFDCLAHGTDD
                     LRESPLDERRRRLEVLLADPPASILLTPATSDATLARRWFDVFEGAGLDGVIAKRWDQ
                     PYRPGKREMAKVKHLRTADCVVAGFRWHKNGPGTLVGSLLLGLWNDAGHLQHVGVTSS
                     FTAARRAELVELLEPYRRDAVEGHPWREWAEWQAAEEVRRMPGATSRWNRGKDLSWEP
                     LRPELVCEVAFDHLQGDRFRHAATFVRWRPDKPPSECRYDQLEETPPALLADLFGGR"
BASE COUNT          539 a         1359 c         1425 g          594 t
ORIGIN      
        1 atcggctccg cgcgggcgtg ccggctcggt tgcgccggcg accgtcagcg gctcctaccg
       61 ccgggcggac ggcaccgttt cgccaaccgg gcgccgtcgc tcgcggcatc cgctgctggt
      121 ggtcgcacag gccaaggcca aggccgatgc gctcgccggg ctggaccgat ggaaggcgcg
      181 ccaccccgag gcggccgcgc acctggccga ggacgacgtg ctggtcgacg gcatgcgcgg
      241 ccgttcctcg ctgtggtacc gcgtccgcgt caacctgcgc catgtccccg aagatgcgcg
      301 gccggatcag gagccacccg acccagacta cgaccccgcc gacgagtggt tgggcgcgga
      361 gagccgcgac tgacccgcgg cgtcagcggt tcaggcgagc tgtcagtcgc tccttcaccg
      421 atggccactc gtcgtccagg atcgagtagt acacggtgtc gcggatgaag ccatctggca
      481 ggcgcaggtg ccgacgcagg accccctcgc ggacgccgcc cagccgctcg atggcgcgct
      541 gtgagcgctc gttgcgcgca tcggtcttaa gcgcgacccg cgtggcgcca agctgctcga
      601 aggcgtacgt cagctgcagg agcttggcct cggtgttgac ggcggtgcgc tggtgtgacg
      661 gcgcgatcca ggtccagccg atctccagcc gcccgtcggc cggcgagatg tcgccgaagc
      721 gggtggaccc gatggcccgg ccgctgacgg tgtccaccgt ggcccaagtg atgaagtcgg
      781 gacgcagcgc gtccgccagg taggccggcc actccgcagc gccacgccac agctggctca
      841 gcgtgtagcg gaacgtttcc ggatcctgcg cggccgcggc cagcaggtcg tcgcgatgac
      901 gttcctcgat cggctccagg cgcacgtgcc tgccggccag cgtgacgcgc tccggcgccc
      961 tcatcgaacg gtccagattc acatcaggtg cgcgtttagc ggtcggcgag gccgctgagg
     1021 cgttcgaatt cacgccgcat gtgaattcgg agccttcgcg ggagtggatc ggtgcatgac
     1081 gcgcatgtcg tgtgaattcg gaccttcggg ctcagcgcgc cggcgagacg acggccgcct
     1141 ccacccagcc ggcgatctcg cggcgaagcg tggccagcgg gagcgagccg tggcccagca
     1201 cctcgtcgtg gaaggcccgc aggtcgaagc ggtcgccgag cttctcggac acctcgcggc
     1261 gcgcccgctc gatctcgcgc tggccgatct tgtacgccag ggcctgggcg ggccacacgg
     1321 tgtatcggtc cacctcgatc tcggcgtcca cgtccggcag cgtgccgcgt tcgtgcatga
     1381 actcgatcgc cttgttgcgc gaccagccca tggcgtgcag gccggagtcg accaccaggc
     1441 gagcggctcg gaaggcctgg gcgtcgagca tgcccagccg ctcccggtcg tccaggtaga
     1501 ggcccatctc atccgccagg cgctcggtgt acaggcccca gccctcgacg taggccacgc
     1561 cggccatgcg tccgccgagg gtgcggaagc gcggcagacc ggtcagctcc atctcgatcc
     1621 cgatctggaa gtggtggccg ggcgtcgcct cgtggaaggt gatggccgcg atcttgtgca
     1681 gctggcgctc ctccggctgg taggtgttga tgtagtactg gccctggcgc ttgccgtcga
     1741 tcgagggctg catgtagaag gcgggcggcg tctcgcgctc gcggtactcc tcgaccggct
     1801 tcaccacgca gttggccgag ggcaggcgtc cgaagtagcg cggcgccgcg gcgtaggcgc
     1861 gctccgtctg ctccacggcg agctgcacga tggcgtcgcg cgagtcggtg tggttcgtcg
     1921 gatccgtctc gagcgcggcc ttcagtgcat ggcggtccgg gtggccgagc cgccgggcga
     1981 tctcgtccat ctcggcctcg atgcgcgcca ggtcctccag cccaaaggcg tgcacctcct
     2041 cggcgctggt cgccaccgtc gtctgcaggc ggatgctgag ccgatacgcc tcggcgccgt
     2101 cgggcgtcga tgacagcccg gggtccgtcc gagcgtgcgc cgcgtagtca tcggccaggt
     2161 agtcccgcag ctgctggagg gcgggcttga cgtgctcgtc cacggccgcc gcgacggcgt
     2221 tgcgcgcgtc gtcgtccgcc acgtgggcca gggtcaccgc cgggaactgc tcggtgggcg
     2281 tggcgaccag ccgctcgatc tgctggacct gcttgtcgac cgggatgcgt gccgcggttc
     2341 gcccgtcggc cacgccctcg cgcagggtct cgatgttctg catcaccgcc gtggggtagg
     2401 cgcggaatcg ggcgagcagc cgctccagcc cgtcgggcga ctcggcgatc tggtactgcg
     2461 ccacggtgac cggcatcagc tgtacgccgg agatgtggtt cacggccagc tggtaaagct
     2521 tgcggtcgag cgcctcgagc tggttgcgcg cgaccagcat cagcatgtcg cgggtgatga
     2581 cctgctcgac gccaagcgcc gccgggtcga tggcctcggc ctcggccagc accccgcggt
     2641 acgcagccgc ctcgtcggca cgtccctgtg cgccgaggtc cggcaggcga tcgtcgtagc
     2701 ggtcgtcgcc caggatggtg gcgaagatag gctcgcgctc caggacgccc tcgaagaagc
     2761 ggtcggcgag ctcgttgacg gcggcgttgg ggtcggggtt ggctggctgc gcggacacgg
     2821 acgtatcggt cctcctggat gggtggctga taaggaggcg agtgtagcga gagccgggat
     2881 gttgccgaga gcaggcttcc cgcgagtgac tgcccctagc ggccgccgaa gaggtccgcc
     2941 agcagcgcgg gcggcgtctc ctccagctgg tcgtagcggc actcggacgg cggcttgtcc
     3001 ggccgccagc ggacgaaggt ggcggcgtgg cggaagcgat cgccctggag gtgatcgaag
     3061 gccacctcgc acaccagctc cgggcgcagc ggctcccagg agaggtcctt gccgcggttc
     3121 cagcgcgagg tggcgccggg catgcgacgc acctcttcgg ccgcctgcca ctccgcccac
     3181 tcgcgccagg gatgtccctc cacggcatcg cgacggtagg gctccagcag ctcgaccagc
     3241 tccgcgcgac gcgccgcggt gaacgaggag gtcacgccca cgtgctgcag gtggccggcg
     3301 tcgttccaca gccccagcag cagcgagccg accagcgtgc ccggcccgtt cttgtgccag
     3361 cggaagccag ccactacgca atcggcggtg cgcaggtgct tgaccttcgc catctcgcgc
     3421 ttcccgggcc ggtagggctg gtcccagcgc ttggcgatca cgccgtccag gcccgcgccc
     3481 tcgaagacgt cgaaccagcg gcgggcaagc gtcgcatccg aggtggccgg cgtgagcagg
     3541 atcgatgccg gcgggtccgc cagcagcacc tccagccggc ggcggcgctc atcgagcggg
     3601 ctctcgcgca ggtcatcggt tccgtgggcc aggcagtcga aggcgacgaa cgaggcgggc
     3661 gtctcaccgg ccagcatggc cacccggctg gcggcgggat ggatgcgcag cagcagcgca
     3721 tcgaagtcca ggccaccgtc gcgcgcgatc acgatctcgc cgtccagcac gaagcaggca
     3781 tcggcggtgc cgatcgcgcg caccgggtcg gcgagctcgg ggaagtatcg gtccagcggc
     3841 ttgaggtcac gcgactggag gtagacctcc tgcccgtcgc ggaagacgat ggcccggaag
     3901 ccgtcccact tcggctc
//
LOCUS       DATACA010000272         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_276248, whole genome shotgun sequence.
ACCESSION   DATACA010000272 DATACA010000000
VERSION     DATACA010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276248"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1327)
                     /locus_tag="VHK28_05605"
     CDS             complement(137..1327)
                     /locus_tag="VHK28_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141738.1"
                     /translation="MAATKVLVVDDDLNIQRVLVFTLKQEGFDVLVASDGQAGVQMAR
                     DAQPDLVLMDVAMPKLDGYAATQQIRAAETDGRRVPIIMLTTEADVGERVKGLRAGAD
                     DDIVKPFHPLELMARIKALLARGGALTQATSPDAATLGQLCVFYGAKGGVGTTTIAIN
                     AAIAMVREFRRRVALVDANLQFGDLRVFLDLGLDTASIVNAIGEPDLDQDLLRKLMVR
                     HHSGIDLLPAPPSPEAADIVMEHQRNNPGELTNLLNLSRRLYDYTLVDMVKTIDDFNL
                     QLFDEADVIFVVMTADLSCLKNVRLVLETMDHLGYEKSKVQLVLNRSNAYTGINVRNA
                     ESALNRKVDYEIINEYRGAISALNSGEPFMSSRPDGPLGRSVTEFAKQVDTALAAQVA
                     AATG"
     gene            complement(1385..>1601)
                     /locus_tag="VHK28_05610"
     CDS             complement(1385..>1601)
                     /locus_tag="VHK28_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2141739.1"
                     /translation="SSRGAISSSPRGDQVVSWTQSLKMYDAAQEPKELFVVDGCGHSE
                     AHAVAGPEYERRVLAFLGRYLDGGTPV"
BASE COUNT          260 a          526 c          548 g          267 t
ORIGIN      
        1 gacacctctg agcgaggcga gcgcgggtga gcgaggtagt gccggccgcc cgcctgctcg
       61 cctcccaaac tccagcggcg accggcgtgc cggagcgtag gagaccgggc cttatcggcc
      121 ccttatcagc cggttatcag ccggtagcag cggcgacctg cgcggccagc gcggtgtcca
      181 cctgcttggc gaactcggtg accgagcgcc ccaatgggcc atcgggccga gaggacatga
      241 acggctcgcc ggaattcagg gcgctgatgg cgccgcgata ctcgttgatg atctcgtagt
      301 cgaccttgcg gttcagcgcc gactctgcgt tgcgcacgtt gatgccggtg tacgcgttgg
      361 accgattgag gacgagctgc accttgctct tctcgtagcc caggtgatcc atggtctcca
      421 gcaccagccg cacgttcttc aggcaggaga gatccgcggt catgaccacg aagatcacat
      481 ccgcctcgtc gaagagctgg aggttgaagt catcgatggt cttgaccatg tcgaccagcg
      541 tgtagtcata cagccggcgg gacaggttca gcaggttcgt cagctcgccg gggttgttgc
      601 gctggtgctc catcacgatg tcggccgcct cgggcgaagg tggggcgggc agcaggtcga
      661 tcccggagtg gtgacggacc atcagcttgc ggagcaggtc ctggtccagg tccggctcgc
      721 cgatggcatt cacgatcgag gcggtgtcca ggcccaaatc caggaagacg cgcaggtcgc
      781 cgaactgcag gttggcgtcc accagcgcca cccggcgacg gaactcgcgg accatcgcga
      841 tggcggcatt gatggcgatg gtggtggtcc ccacgccgcc cttggcaccg tagaagacgc
      901 acagctgacc gagggtggcg gcatccggcg aggtggcctg cgtcagcgca ccaccccgcg
      961 cgagcagggc cttgatgcgt gccatcagct ccagcgggtg gaacggcttg acgatatcgt
     1021 cgtccgcgcc ggcacgcagg ccctttaccc gctcgccgac gtccgcctcg gtggtgagca
     1081 tgatgatcgg gacccgccgt ccatcggtct cggccgcgcg aatttgctgg gtcgccgcgt
     1141 acccgtcgag cttgggcatg gcgacatcca tcaggaccag gtccggctgg gcgtcgcggg
     1201 ccatctgcac gcccgcctgt ccatcggagg caacgaggac gtcgaagccc tcctgcttga
     1261 gggtgaagac gaggacgcgc tggatgttca ggtcgtcatc gacgacaagg accttcgtgg
     1321 cagccatcaa ggaccccagg tggatgtgga tgggcgtcgc aggcgcgccg tgcgccggcc
     1381 gagattatac gggcgtgcca ccgtccaggt agcgccccag gaaggcgagc acgcgccgct
     1441 cgtactccgg gccggcgacc gcgtgcgcct cgctgtgccc gcagccgtcc accacgaaca
     1501 gctccttcgg ctcctgggcg gcatcgtaca tcttcagcga ctgcgtccag gagaccacct
     1561 ggtcgccccg cgggctgctg cttatcgcgc cgcgcgacga c
//
LOCUS       DATACA010000273         4609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2847019, whole genome shotgun sequence.
ACCESSION   DATACA010000273 DATACA010000000
VERSION     DATACA010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847019"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHK28_05615"
     CDS             complement(<1..195)
                     /locus_tag="VHK28_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141740.1"
                     /translation="MTPRDDEPAEGPNGRPAASLGELDELGRLDALDELEPVVDPSGW
                     FGVQLRYAYLVGESQSVVMIE"
     gene            complement(192..1787)
                     /locus_tag="VHK28_05620"
     CDS             complement(192..1787)
                     /locus_tag="VHK28_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2141741.1"
                     /translation="MAATTPRMKITYATLSADNEELQAAFDAAVEQVRGALGRSYPMI
                     IGAEERRADQEFEDRSPIDREIVVSRFPVGTRQDVQDAIAAARKAFPAWRDLGWRKRI
                     ELMHRAADLISERQFEYAALMAFEVGKNRLEALGDVEETADLLRYYSDEMERNEGFLK
                     PMGSLSPTEHTRSILKPHGVWAVISPFNFPMALAGGPAAGAMIAGNTVVLKPSSDAPL
                     MAYKFGEVLREAGVPAGVFNLVTGPGETVGAELEENPGIDGMVFTGSYEVGMKLQRGF
                     TRDYPRPIITEMGGKNPAIVTASADLDAAAEGVMRSAFGFDGQKCSAASRVYVENRVA
                     RDFVERLVDRARQISVGDPTRRESWMGPVINERALNTFTAAVEEARRDGGTIEVGGEV
                     KADGDEGRGYFPTPTVVTGLPLEHRLFREELFVPFLVVGEVDSLDQALEEANATRYGL
                     TAGIYSSREDEIRRFLDTIQAGVVYVNRRAGATTGAWPGIQSFGGWKGSGSSGKSGLG
                     PYYVQQFLREQSQTVMGQEPPPA"
     gene            complement(2011..2730)
                     /locus_tag="VHK28_05625"
     CDS             complement(2011..2730)
                     /locus_tag="VHK28_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2141742.1"
                     /translation="MTVRAILPAWTDADPDLHARLGDVFDPDRRVLEALERITPLSGR
                     RIADVGTGIGHYPMLLARRTGRTYGIEADDGLRQEARRRAAAMHQPNIRIVAGEPTRL
                     PLRDGAVDIVLSGLIDAEDGSLPAIGEAMRVLRPGGRLVVIGYYGRDDLASLLEPEVV
                     AHALEATRRRTGWWLRNGFKIKVVHIRIDLHDPDVALELLPRLYGDRGRAYLMGPHPS
                     QLQVRLGLYHRGVDDPPPAAT"
     gene            complement(2741..3412)
                     /locus_tag="VHK28_05630"
     CDS             complement(2741..3412)
                     /locus_tag="VHK28_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141743.1"
                     /translation="MPDSAAAVLAGRGLRRVLLTVVQAALLVACEAAPSPSPSATASP
                     QPTPTTTSVVSASAIPTGSPTATPEPPLSLELPDERDDRQLRVALQPEVPADGDGRIE
                     VSVTNLSGERVDELVLRWPTELADAVFLAPFEPTPGRIAVGGPPLIQPWTKWVIGPGE
                     RGEPAGTTSLGWGPLDPGATLTIPLVASRRAPGPLEFDLQLLAGEALLTLEGGEPAEL
                     RVAVP"
     gene            complement(3405..4088)
                     /locus_tag="VHK28_05635"
     CDS             complement(3405..4088)
                     /locus_tag="VHK28_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2141744.1"
                     /translation="MSVSAEPQIRILIADDHEVVRVGLAALLQRQDGFQVVGEAASGE
                     EAVRLARALRPDVVVMDIRMPDGSGTDACRTITDELPGSPVVMLTSYADEDALFDAIA
                     AGASGYVLKRIGSAELVDAVRTVAGGQSMLDPEVTAAVLHRLRRAAHAEESDVFRELT
                     EQERRVLAHVAEGLSNREIAAAMDLAEKTVRNYVSSILAKLALTSRSQAAAFAIRNRL
                     TELSRRANA"
     gene            complement(4117..>4609)
                     /locus_tag="VHK28_05640"
     CDS             complement(4117..>4609)
                     /locus_tag="VHK28_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2141745.1"
                     /translation="ELEHISGELRRTIFDLRTASLETLDPEEIVRSVADELRANSLVA
                     VDLQVTGDWTPVLSPEQAEQLRQIVHEAFSNVLRHARAGRVSVRLTTSRRRLHLEVGD
                     DGIGFDPDLAAERGRTGRAQGLANIRTRAALLRATLRLETTPGQGTRLSLTMPVSATR
                     RRT"
BASE COUNT          647 a         1623 c         1652 g          687 t
ORIGIN      
        1 ctcgatcatg accacgctct gcgactcgcc gaccaggtag gcgtagcgca gctggacccc
       61 gaaccagccg ctgggatcga ccaccggctc cagctcgtcc agcgcgtcca gccggccgag
      121 ctcatcgagc tcaccgagcg acgcggccgg gcgtccgttt gggccctcgg cgggctcgtc
      181 gtcgcgcggc gtcacgcggg cgggggctcc tggcccatga ccgtctgcga ctgttcgcgc
      241 aggaactgct ggacgtagta cgggcccagg ccgctcttgc ccgacgagcc agagcccttc
      301 cagccgccga agctctggat gccgggccac gcgccggtgg tcgcccccgc gcggcggttg
      361 acgtacacca cgccggcctg gatggtgtcc agaaagcgcc ggatctcgtc ctcgcggctg
      421 ctgtagatgc ccgccgtcag gccgtacctg gtggcatttg cctcctccag cgcctggtcc
      481 agggaatcga cctcacccac caccaggaac ggcacgaaga gctcctcgcg gaagagccga
      541 tgctcgagtg gcagcccggt gacgacggtg ggggtcggga agtagccacg cccctcgtcg
      601 ccgtcggcct tcacctcgcc gccgacctcg atcgttccgc cgtcccgacg cgcttcctcg
      661 acggcggcgg tgaaggtatt caacgcgcgc tcgttgatca ccggtcccat ccagctctcc
      721 cgccgggtgg ggtccccgac gctgatctgg cgcgctcgat cgaccaggcg ctcgacgaag
      781 tcgcgtgcca ccctgttctc gacgtagacc cgtgatgcgg cgctgcattt ctggccgtcg
      841 aagccgaagg ccgagcgcat cacaccctcg gcggcggcgt ccaggtcggc ggaggcggtg
      901 acgatggcgg ggttctttcc gcccatctcg gtgatgatcg gtcgcgggta gtcgcgggtg
      961 aagccgcgct gcagcttcat gcccacctcg tagctcccgg tgaagaccat gccgtcgatg
     1021 cccgggttct cctccagctc ggcgccgacc gtctccccgg ggccggtgac caaattgaag
     1081 acccccgccg gcacgcccgc ctcgcgcagg acctcgccga acttgtaggc catcaacggc
     1141 gcgtcggagg agggcttgag cacaaccgtg ttgccggcga tcatcgcgcc ggcggccggg
     1201 ccgccggcga gggccatcgg gaagttgaac gggctgatga ccgcccacac gccgtgcggc
     1261 ttgaggatgg atcgcgtgtg ctcggtgggt gagagggacc ccatcggctt caggaagccc
     1321 tcgttgcgct ccatctcgtc cgagtagtag cgcagcaggt ccgccgtctc ctccacgtcg
     1381 cccagggcct cgagccggtt cttcccaacc tcgaaggcca tcagcgcggc gtactcgaac
     1441 tgccgctcgc tgatgaggtc ggcggcgcgg tgcatcagct cgatgcgctt ccgccagccg
     1501 aggtcgcgcc aggcggggaa cgccttgcgc gcggcggcga tggcgtcctg cacgtcctgg
     1561 cgagtgccga ccgggaagcg cgagactacg atctcgcggt cgatcggcga gcggtcctcg
     1621 aactcctggt cagcgcgccg ttcctcggcg ccgatgatca tcggatacga gcggccgagc
     1681 gctccgcgaa cctgctccac ggccgcgtcg aaggctgcct ggagctcctc gttgtccgca
     1741 gacagcgtgg cgtaggtgat cttcatgcgg ggagtggtgg cggccatgtg gggcaatccc
     1801 tccagctctg tcgagcggcc tgcgggcacc gatgcctgcc gcagaggtgg tcattatcgc
     1861 accgagttcc gtggagcacg ccgaaggcca ggctccgggg ggagaagagc ctggccttcg
     1921 accgaccgat gatagaggca ccgattcccg gccgcaagag gattcctgca ctgaatccgc
     1981 attcgcggtg acgggatgta acggatcggg ttacgttgcc gccggcggcg ggtcgtcgac
     2041 cccgcggtgg tagaggccga gtcgcacctg gagctgcgac gggtgcgggc ccatcaggta
     2101 cgcgcggcct cgatcgccgt acagccgggg gagcagctcc agcgcgacgt ccgggtcgtg
     2161 gagatcgatg cggatgtgca cgaccttgat cttgaaaccg ttgcgcagcc accacccggt
     2221 gcgtcgccgg gtcgcctcca gggcgtgcgc caccacctcg ggctcgagga ggctggccag
     2281 gtcatcccgc ccgtagtaac cgatcaccac cagccgcccg ccggggcgca ggacgcgcat
     2341 cgcctcgccg atggccggca gcgagccgtc ctccgcgtcg atcaggccgg agagcacgat
     2401 gtcgaccgcc ccgtcccgca gcggcagacg ggttggttcg ccggccacga tccggatgtt
     2461 cggctggtgc atggcggccg cgcggcgtcg cgcttcctgc cgcaggccgt cgtccgcctc
     2521 gatcccgtag gtgcgtcccg tgcggcgtgc cagcagcatg gggtagtggc cgatcccggt
     2581 gccgacgtcc gcgatgcgac gcccggagag cggcgtgatg cgttcgagcg cttctaggac
     2641 ccggcgatcg ggatcgaaca cgtcgccgag ccgcgcatga aggtcgggat ccgcatcggt
     2701 ccaggccggg aggatggcgc gcaccgtcat gccggcaagc ctacggcacg gcgacccgca
     2761 gctcggccgg ttcaccgccc tccagggtca gcagcgcctc tccggccagc agctggaggt
     2821 cgaactcgag cggccctggc gcccgccggc tggcgaccag gggaatggtc agcgtggcac
     2881 ccgggtcgag cggtccccat cccagggatg tggtgccggc cggctcgcca cgctcgcctg
     2941 gaccgatgac ccacttggtc cacggctgga tgagcggtgg cccgccgacc gcgatccggc
     3001 ccggcgtggg ctcgaagggc gccaggaaca cggcgtccgc cagctcggtc ggccagcgca
     3061 ggacgagctc gtcgacgcgc tcgccggaca ggttcgtgac gctgacctcg atccggccgt
     3121 caccgtcggc gggtacctcc ggctggagcg cgaccctcag ctggcgatcg tcacgctcgt
     3181 cgggcagctc cagcgagagc ggcggctcgg gagtggccgt cggtgagccg gtcgggatgg
     3241 cactggccga cacgacggag gtcgttgtcg gcgtcggctg cgggctggcc gtcgccgacg
     3301 ggctgggcga gggcgcggcc tcgcaggcga ccagcaacgc tgcctggacg acggtcagca
     3361 gtacccggcg gaggccccgc ccagccagca ccgccgcggc ggagtcaggc attcgcgcgc
     3421 cgggacagct cggtgagtcg attgcgaatg gcgaatgcgg cggcctgcga gcggctggtc
     3481 agcgccagct tggccagaat gctgctgacg tagttgcgca cggtcttctc ggccaggtcc
     3541 atggcggccg cgatctcgcg gttggacagg ccctcggcga cgtgcgcgag gacccgccgc
     3601 tcctgctcgg tgagttcgcg gaagacgtcc gactcctcgg catgggccgc ccgccgcagg
     3661 cgatggagca cggcggcggt cacctccggg tccagcatcg actggccgcc ggccacggtc
     3721 cgcaccgcat ccaccagctc agccgacccg atgcgcttga ggacgtagcc ggaggcgcct
     3781 gcggcgatgg catcgaacag cgcgtcctcg tcggcgtagc tagtgagcat cacgaccggg
     3841 ctcccgggca gctcgtcggt gatggtgcgg caggcatcgg tgccggatcc atccggcatg
     3901 cggatgtcca tgaccaccac gtcggggcgg agggcgcgag ccagccgcac ggcctcctcg
     3961 cccgaggcgg cctcgccaac cacctggaag ccatcctggc gctggaggag cgcggccagg
     4021 ccgacccgga cgacctcgtg atcgtcggcg atcaggatgc ggatctgggg ctcggcgctc
     4081 acgctcatgg gtgtaccgcg cttgggtgta ccgcgttcac gttctccgcc gggtggcgga
     4141 caccggcatg gtaagcgaga ggcgggtgcc ctgccccggg gtggtctcga gccgcagcgt
     4201 ggcccgcagc agcgccgccc gggtgcggat gttggccagg ccctgggcac gcccggtgcg
     4261 gccgcgctcg gccgccaggt cgggatcgaa gccgatcccg tcgtccccca cctccaggtg
     4321 gaggcgccga cgggatgtcg tgagccgcac gctgacgcga cccgcgcggg cgtgacgcag
     4381 gacgttgctg aacgcctcgt gcacgatctg gcgcagctgc tcggcctgct cgggcgagag
     4441 gaccggcgtc cagtcgccgg tgacctgcag gtcgaccgcc accagactgt tggcgcgcag
     4501 ctcgtccgca acgctgcgca cgatctcctc cggatcgagc gtctcgaggc tggcggtgcg
     4561 caggtcgaag atcgtgcgcc gcagctcgcc actgatgtgc tccagctcg
//
LOCUS       DATACA010000274         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_758347, whole genome shotgun sequence.
ACCESSION   DATACA010000274 DATACA010000000
VERSION     DATACA010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758347"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHK28_05645"
     CDS             <1..183
                     /locus_tag="VHK28_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2141746.1"
                     /translation="TGLDLKMEQYEAGERFVKAVIAARGREFLNRVWEGPAQLPSLEE
                     IRAADRWIGRIEEARP"
     gene            150..1184
                     /locus_tag="VHK28_05650"
     CDS             150..1184
                     /locus_tag="VHK28_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX2141747.1"
                     /translation="MDRPHRGGASLTGPSSGEAVVLATPIFGGPLSAQHVARTEQVPG
                     VRVVPISREGVVHADDPEAALAAARVVLRGGVPASVLDHLIARAPRLEWIHSFSAGVD
                     RVATPSVREHGLKVTNARGVFSRPIAEYVVMWCLAIARRLPQLLELQRERTWQPLRGR
                     ELASLTLGVVGYGSIGSELARLVAPFGTQVVATRRRPELGAQEPNVQLLGHDQLDELL
                     GVCDVVVVAAPLTESTAGMIGAPQLQAMRQGAWLINIARGRLLDELALRRALESGWIA
                     GAVLDVFNEEPLPPDSPLYATPNLYVTPHTSWSSDRVVERSVDLFVDNLRRFVSGQPL
                     ENLVDLEAGY"
     gene            1189..>1815
                     /locus_tag="VHK28_05655"
     CDS             1189..>1815
                     /locus_tag="VHK28_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2141748.1"
                     /translation="MQVTIVGLPGAGKTTVFNALTGGHAETGGYSGGRAAPNIGVVKV
                     PDERLDRLSELFRPRKTTPADVTYVDVAIPAGAAREGTVQPDVLAQIRNADALLHVAR
                     AFEGGDRPADPWRDVEEMELEFTVADLTVVQKRLERLATSGRHGSPSEREQNAQEEEL
                     LRRLEPHLSDGLPLRTFSLTDDEEHRLRGYRFLTQKPVLVVLNIDEARL"
BASE COUNT          250 a          631 c          669 g          265 t
ORIGIN      
        1 accggcctgg atctgaagat ggagcagtac gaggcggggg aacggttcgt gaaggcggtg
       61 atcgccgccc gcgggcgcga gttcctgaac cgggtctggg agggtccggc gcagctgccc
      121 agcctggagg agatccgggc agcagaccga tggatcggcc gcatcgagga ggcgcgtccc
      181 tgaccgggcc gagcagcggc gaggcggtgg tcctggccac gccgatcttc ggcgggccac
      241 tgtcggcgca gcacgtggcc cgcaccgaac aagtgcccgg cgtgcgggtg gttcccatca
      301 gccgcgaggg cgtggtccac gccgacgacc cggaggccgc cctggcggcg gcgcgcgtcg
      361 tgctgcgcgg cggcgtgccg gcatcggtcc tggaccacct gatcgcacgc gctccgcggc
      421 tggagtggat ccattccttc tcggcgggcg tcgatcgtgt cgccactcca tcggtccgtg
      481 agcacgggct gaaggtgacc aacgcgcggg gcgtcttcag ccggccgatc gccgagtacg
      541 tggtcatgtg gtgcctggcc atcgcccgcc gcctgccgca gctgctggag ctgcagcggg
      601 agcggacttg gcagcccttg cgcggcaggg agctcgccag cctgaccctg ggcgtggtgg
      661 gctacgggag catcggctcc gagcttgcgc ggctggtcgc tccgttcggc acgcaggtgg
      721 tcgccacccg gcggcgcccc gagctcggcg cacaggaacc gaacgttcag ctccttggcc
      781 atgaccagct ggacgagctg ctgggcgtct gcgacgtggt ggtggtcgcc gccccgctga
      841 ccgagtcgac ggccggcatg atcggcgcgc cgcagctgca ggccatgcgc cagggcgcgt
      901 ggctgatcaa catcgcgcgc gggcgcctgc tggatgagct ggcgctgcgc cgtgctctgg
      961 agtcgggctg gatcgccggg gccgtgctcg acgtcttcaa cgaggagccg ctgccgcccg
     1021 actccccgct gtacgccacg ccgaacctgt acgtcacgcc gcacacctcc tggtcctccg
     1081 accgggtggt ggagcgctcc gtcgacctgt tcgtggacaa cctccgccgc ttcgtgagcg
     1141 gacagccgct cgagaacctg gtggacctgg aggccgggta ctagaccgat gcaggtcacc
     1201 atcgtggggc tcccgggcgc cggcaagacc acggtcttca acgccctcac cggcggtcac
     1261 gccgagaccg gcggctactc cggcgggcgc gccgccccga atatcggcgt ggtcaaggtg
     1321 cccgacgagc gcctggaccg cctgtcagag ctgttcaggc cgcgtaagac gacgccggcc
     1381 gatgtcacct acgtggacgt ggccatcccg gccggcgcgg cgcgggaggg gaccgtccag
     1441 ccggacgtgc tggcccagat ccgcaatgcg gacgcccttc tccacgtggc gcgtgccttc
     1501 gagggcggcg accgcccggc ggatccctgg cgagacgtcg aggagatgga gctggagttc
     1561 accgtcgctg acctgaccgt cgtccagaag cggctggagc ggctggcgac ctccggccgc
     1621 cacggatctc cctccgagcg cgagcagaac gcccaggagg aggagctgct gcgccggctt
     1681 gagcctcacc tctcggacgg gctaccgctg cgcaccttct cgctgaccga tgacgaggag
     1741 caccgcctgc gcggttaccg cttcctgacg cagaagccgg tgctcgtggt gctcaacatc
     1801 gacgaggcgc ggctg
//
LOCUS       DATACA010000275         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2043909, whole genome shotgun sequence.
ACCESSION   DATACA010000275 DATACA010000000
VERSION     DATACA010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043909"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VHK28_05660"
     CDS             complement(<1..992)
                     /locus_tag="VHK28_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2141749.1"
                     /translation="MTDRTLIRGGIVLTQDDKLGEMPNGDILIEDGVIVDVADGLNAD
                     GARVIEADGDIVIPGFIDTHRHTWETCLRTCAPDYPLITYFSAILDKFAPHYRPEDVH
                     SATLWGALECLNAGITTLVDWAHIMNTPEHADASVRALQESRIRSVFAYGFPNTSLQD
                     WWFGPDYAGSVLTSNGDDARRVKAQYFSSDDGRITMGLATRGPGFCKPEVVRHDWELA
                     KELCVPITVHVGMDRFGYTKGQISALRDMDLLYPGTTYVHASHFSDEEWALVRDSGGN
                     VSFAPQIEIQMGHGWAPAVTALEYNVPISLSSDVATTASADQFTQMHAIFGSERG"
     gene            complement(1026..>1778)
                     /locus_tag="VHK28_05665"
     CDS             complement(1026..>1778)
                     /locus_tag="VHK28_05665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2141750.1"
                     /translation="LGARVVDTPAAAAAEADVAISMLADGPAVASVWEGEDGLLAGAR
                     QGSVLVDMSTVPPETLRGYEEVARAAGAGILDAPVSGSVALAESGGLTIMAGGEAADL
                     ERARPVFDQLAKQVFHLGPLGSGAAMKLAVNAVIFGLNNALSEGLVLAERAGVDRELA
                     YDVLANSAAGAPFVGYKRAAFLEPDATPVAFALELAEKDLRLIAELADAVGASVPQAR
                     TNLELIRAAAATVGGDRDFSAVASHLRSQAEP"
BASE COUNT          255 a          619 c          633 g          271 t
ORIGIN      
        1 ccgcgctcgg acccgaagat ggcgtgcatc tgcgtgaact ggtcggccga tgcggtggtg
       61 gccacgtccg acgagagcga gatcggcacg ttgtactcca gcgcggtgac cgccggcgcc
      121 cagccgtggc ccatctggat ctcgatctgc ggcgcgaagc tcacgttgcc gcccgagtcg
      181 cggaccagcg cccactcctc gtcgctgaag tgcgaggcgt ggacgtaggt ggtgccgggg
      241 tacagcaggt ccatgtcgcg cagcgcgctg atctggccct tcgtgtagcc gaagcggtcc
      301 atgcccacgt gcaccgtgat cgggacgcac agctccttgg ctagctccca gtcgtggcgc
      361 acgacctccg gcttgcagaa gcccgggccg cgggtcgcca atcccatggt gatgcggccg
      421 tcgtccgagc tgaagtactg ggccttgacg cgtcgcgcat cgtcgccgtt gctggtcagg
      481 accgagccgg cgtagtcggg gccgaaccac cagtcctgca gcgaggtgtt cgggaacccg
      541 taggcgaaga cggaccggat ccgggattcc tgcagggcgc ggacggaggc atcggcgtgc
      601 tcgggggtgt tcatgatgtg tgcccagtcg accagcgtgg tgatgccggc gttcaggcac
      661 tccagcgcgc cccacagcgt ggcggagtgc acgtcctccg gccggtagtg cggcgcgaac
      721 ttgtccagga tggcgctgaa gtaggtgatc agcgggtagt ccggcgcgca ggtccgcagg
      781 caggtctccc aggtgtggcg gtgggtgtcg atgaatccgg ggatgacgat gtcgccatcg
      841 gcctcgatga cccgggcgcc atcggcattc agcccgtccg ccacgtccac gatcacgccg
      901 tcctcgatga ggatgtcgcc attcggcatc tcaccgagct tgtcgtcctg ggtcaggacg
      961 atgccaccac ggatcagggt tcggtcagtc acggatcggt ccctttctgc tgcgggttga
     1021 aggggtcatg gttcggcctg cgagcgcagg tgcgaggcga ccgccgagaa gtcgcggtct
     1081 ccccccaccg tggcggcggc ggcgcggatg agctccaggt tggtgcgggc ctgggggacg
     1141 gaggccccga ccgcatcggc cagctcagcg atcaggcgca ggtccttctc ggccagctcc
     1201 agggcgaagg cgaccggcgt ggcatccggc tccaggaagg cggcccgctt gtagccgacg
     1261 aacggggcac cggccgcgct gttggccagg acgtcgtagg ccagctcgcg gtcgacgccg
     1321 gcccgctcgg cgagcaccaa accctccgaa agggcgttgt tcagcccgaa gatgacggcg
     1381 ttgacggcca gcttcatggc cgccccgctg cccagcggcc ccaggtggaa gacctgcttg
     1441 gccagctggt cgaacacggg gcgggcgcgc tccaggtcgg cagcctcccc gccggccatg
     1501 atggtcaggc cgccggactc ggccagcgcc acgctccccg acacgggtgc gtccaggatg
     1561 ccggctcccg ccgcgcgggc gacctcctcg tagccccgca acgtttccgg gggcacggtg
     1621 ctcatgtcca ccagcaccga cccctggcgc gccccggcca gcagcccgtc ctccccctcc
     1681 cagaccgatg ccaccgccgg cccgtcggcc agcatgctga tggcgacgtc cgcctccgcc
     1741 gcggccgcag cgggcgtgtc gaccacacgc gcgccgag
//
LOCUS       DATACA010000276         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2123625, whole genome shotgun sequence.
ACCESSION   DATACA010000276 DATACA010000000
VERSION     DATACA010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123625"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VHK28_05670"
     CDS             <1..507
                     /locus_tag="VHK28_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2141751.1"
                     /translation="AGKLGAVFLQYPRWFFPSNESRDAIVEAHERLAGFGITAAVEFR
                     NGTWFNEKNAERTVQFLEKYAIPFVMVDEPQTKTSLPPIVAVTSPDLAVVRFHGRRVE
                     TWEAKGIPVVERFRYLYDEQELGEWVPRVRQAAEAARNVHVLMNNCYANYGTTNAREL
                     AALIQSDS"
     gene            complement(519..1001)
                     /locus_tag="VHK28_05675"
     CDS             complement(519..1001)
                     /locus_tag="VHK28_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX2141752.1"
                     /translation="MERTLRRTDGLATTRAPADVELLAWLLDNSILIPGTRWRVGLDA
                     LVGLVPGVGDVVSGLFGLMVVGRAAQLGLPRVVLARMLANVALDFAFGSVPLAGDAFD
                     AWYKAHARNVNLIRRHLDAPRASTASHWLFFAGLLAVFVLLAVGIVWLIGSLLGALFG
                     "
     gene            1064..1912
                     /locus_tag="VHK28_05680"
     CDS             1064..1912
                     /locus_tag="VHK28_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX2141753.1"
                     /translation="MTFRKGVRLRPGQVRDLRGAGGVAVGGGIGGILLLAFFLLMGGN
                     PGDVPLGSLAGRTVGTGQANDLSDECQTGEDANQRRDCRIVGFVNSIQEYWSGALDGY
                     QLAPTTFFEGGVQTGCGNASSAVGPFYCPGDQGVYIDLGFFDQLESRFGAEGGPLAEA
                     YVIAHEYGHHVQNLIGVLRPGGGGSGAESDAVRTELQADCFAGVWAFHAVDTGYLEPI
                     TEDQVGQAVSAAEAVGDDRIQEQVQGQVNPETWTHGSSDQRRSWFLTGMNSGDPNECD
                     TFEAEL"
     gene            1982..2386
                     /locus_tag="VHK28_05685"
     CDS             1982..2386
                     /locus_tag="VHK28_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141754.1"
                     /translation="MAGTILIVFGSLLAAVGAVLLVLSFVFSSDAPLPAWIAVDPLVV
                     DPGTGLLLLGLALMTIGVSQVVAGVAAMTRGLGWARPAGIGLALPGAGAATWALLPLE
                     PTPSVQLIFVPVIAAYLFVVLAEALAPDWFRG"
     gene            complement(2450..2935)
                     /locus_tag="VHK28_05690"
     CDS             complement(2450..2935)
                     /locus_tag="VHK28_05690"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX2141755.1"
                     /translation="MAYDTTQRTGGSAGTMTAPSSATWNLVRAGDLQGRSIIGLNDES
                     IGTVGDVFLDYDEHTIRYATVDVGGFLGMGAKTVLVPFEKLQWSSEDLYLPVEKSVLE
                     NAPEFDPTADYDRTYEEQVSGAWGIGPYWTTPEYGVRHSHWREGYDRPMAGYRHQDRS
                     L"
     gene            3109..3522
                     /locus_tag="VHK28_05695"
     CDS             3109..3522
                     /locus_tag="VHK28_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141756.1"
                     /translation="MDPLGLLETIFVGATVAGALTVSVLVLGVLGIAARGGVRHMEDF
                     PYPGVTARAEEVESRLRHRYLMRTGTLPVRPAASDQAFTFEPDPEPAPQPVLVPAASG
                     GGFTAALMVREATESPRRGRRGRRSRGRDKPKVAA"
     gene            3592..4638
                     /locus_tag="VHK28_05700"
     CDS             3592..4638
                     /locus_tag="VHK28_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX2141757.1"
                     /translation="MDVIDLRSDTVTRPSAAMRRAMAEAEVGDDQYGEDPSVNRLQAE
                     AAELLGKDSALLLPSGTMANQVALRTLTHSGDDVLAPHEPHIIFHETGAAAANAGVQL
                     RFIGGRDGRFTADEFAAACIPRGHLTSPPTTLVVIENTHNRAGGTIFPLTDVSAVSAA
                     ARDRGVATYCDGARLLNAAVATGRPPAELAAPFDLVAISLSKGLGAPFGSLLAGSREL
                     IASATRHRRMLGGALRQIGILAAAGSVALQHNLGRLVDDHAAARAIADVLAGSPAIAL
                     EPGNVETNIVVFGLTDAAGVPDAAAFVDGCRQRDVLLFAFGPRMLRAVTHLDVSREQC
                     LAAAERMVAVAEGR"
     gene            complement(4635..4838)
                     /locus_tag="VHK28_05705"
     CDS             complement(4635..4838)
                     /locus_tag="VHK28_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141758.1"
                     /translation="MTKRARDEGAVDHWKGGTVAPRSHNLERGRGAESRSEERLAEPG
                     APHQEPSGRELRRAEERERRKKG"
     gene            complement(4875..>5184)
                     /locus_tag="VHK28_05710"
     CDS             complement(4875..>5184)
                     /locus_tag="VHK28_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2141759.1"
                     /translation="ADVGPDDTAFSQRDARFEFVTMAGWEDPAEDEARMTASRRYAAA
                     LQPFASGAYVNGLSDEGASGLRQAYRERTLARLTALKDRYDPDNVFHRNHNVPPSGAG
                     "
BASE COUNT          733 a         1795 c         1827 g          829 t
ORIGIN      
        1 gccgggaagc tgggcgcggt cttcctccag tacccgcgct ggttcttccc ctcgaacgag
       61 agccgggatg cgatcgtcga ggcccacgag cgcctggccg ggttcgggat caccgccgcc
      121 gtcgagttcc gcaacggcac atggttcaac gagaagaacg cggaacgcac ggtgcagttc
      181 ctggagaagt acgcaatccc gttcgtcatg gtcgacgagc cacagaccaa gacctcgctg
      241 ccgccgatcg tggcagtcac ctcgccggac ctggccgtgg tccggttcca cggccggcgc
      301 gtcgagacct gggaggccaa gggcattccg gtggtggagc gcttccgcta cctgtatgac
      361 gagcaggagc tcggcgagtg ggtgccgcgc gtccgccagg cggctgaggc ggcccgcaac
      421 gtccacgtgc tcatgaacaa ctgctacgcc aactacggca ccaccaacgc ccgtgagctg
      481 gcggcactga tccagtcaga ctcgtaacgc cttggggctc agccgaaaag cgctcccagc
      541 agggaaccga tgagccacac gatcccgacc gcgagcagga cgaacacggc cagcagccca
      601 gcgaagaaga gccagtgact ggcggtcgag gcgcggggcg cgtcgaggtg gcgccggatc
      661 aggttgacgt tgcgggcatg ggccttgtac caggcatcga acgcgtcccc ggcgagcggg
      721 accgagccga aggcaaagtc gagcgccacg ttggccagca tgcgggccag caccactcgc
      781 ggcaggccga gctgcgctgc ccgtcccacg accatcaggc cgaacaggcc gctgaccacg
      841 tcgccgacgc ccggcacgag gccgaccagg gcgtccaggc cgacccgcca gcgcgtcccg
      901 gggatgagga tgctgttgtc gagcagccag gcaagcagct ccacgtccgc cggcgcgcgg
      961 gtggtcgcca ggccatcggt cctgcgaagc gttcgttcca tgccgttgca tgtgatcaat
     1021 cgccgtgcca gcgctggccc gccacttgct tcttgggcag ctcgtgacgt tcaggaaggg
     1081 ggtccggctg cggccgggcc aggtccgcga cctgcgaggg gccggcggcg tggccgtggg
     1141 gggcggtatt ggcggcatcc tcctgctggc cttcttcctg ttgatgggcg gaaaccctgg
     1201 ggacgtgcca cttggctcgc tcgcaggccg aacggtgggc accggccagg ccaacgacct
     1261 gtcggacgag tgccagaccg gcgaggacgc caatcagcgg cgtgactgcc ggatcgtggg
     1321 tttcgtcaac agcatccagg agtactggag cggcgcgctc gacggctacc agctggcccc
     1381 gaccactttc ttcgagggtg gcgtacagac cgggtgcggc aacgccagca gcgcggtcgg
     1441 tcccttctac tgtcccggcg accagggcgt ctacatcgat ctcgggttct tcgaccagct
     1501 ggagtcgcga ttcggggcgg agggcggccc gctggcggag gcgtacgtga tcgcgcacga
     1561 gtacggccac cacgtccaga acctgatcgg cgtcctgcgg ccgggcggcg gtggcagcgg
     1621 cgccgagtcg gacgcggttc gcacagagct ccaggccgac tgcttcgccg gggtgtgggc
     1681 cttccacgcg gtcgacaccg gctacctgga gccgatcacc gaggatcagg tcggccaggc
     1741 ggtgagcgcc gcggaggccg tcggtgacga ccgcatccag gagcaggtgc agggacaggt
     1801 caacccggag acctggaccc acggttcgtc cgaccagcgg cggagctggt tcctgaccgg
     1861 catgaactcc ggagacccga acgagtgcga caccttcgag gctgagctgt gaggtcggcg
     1921 gccggactat agtgtcccgg tggaggtgac ccgccggtca gagcgtcccg gagaggttac
     1981 cgtggcaggc acgatcctca tcgtctttgg gtcgctgctg gcggcggtcg gtgccgtgct
     2041 gctggtcctc tccttcgtct ttagctcgga cgcgccgcta ccagcctgga tcgccgtcga
     2101 tccgctggtc gtggaccccg gcaccggcct cctgctgctg ggcctggccc tgatgacgat
     2161 aggcgtttcg caggtggtcg caggtgtcgc cgccatgacg cgcggactgg gctgggcacg
     2221 accggcgggc atcgggctcg cgctgccggg agcgggcgcg gccacctggg cgttgctgcc
     2281 cctggagccc acgccatcgg tccagttgat cttcgtgccg gtgatcgccg catacctgtt
     2341 cgtcgtcctg gcggaggctt tggcacccga ctggttccgc ggctgaacct gtagaagggc
     2401 cgggccgctt gccgcgaccc aagcccggga acggttcgct cgccgtccct tagagggagc
     2461 ggtcctggtg tcgatagccg gccatcggcc ggtcatagcc ctcgcgccag tgcgagtggc
     2521 gaacgccgta ctccggcgtg gtccagtagg gaccgatgcc ccatgccccg ctgacctgct
     2581 cctcgtaggt gcggtcgtag tcggccgtgg ggtcgaactc cggcgcgttc tcaaggacgc
     2641 tcttctcgac cggcaggtac aggtcctcgc tgctccactg cagcttctcg aacgggacca
     2701 gcacggtctt ggcgcccatc cccaggaaac caccgacatc cacggtggcg tagcggatgg
     2761 tgtgctcgtc gtagtccagg aagacgtcgc ccaccgtccc gatcgactcg tcgttcaggc
     2821 cgatgatgct gcgcccctgc aggtcgcctg cacggaccag gttccaggtc gccgacgacg
     2881 gggcggtcat cgtgccggcg ctgccaccgg tgcgctgggt ggtgtcgtag gccattctcg
     2941 gtaccccctt ttgtactgcg tttccgacgc cttccagggc ctgcagggtc catgcccgct
     3001 ggcggtcttt gcgatgacag ccggcaacga ttgggggtca gatagcgtcc tccgggtgca
     3061 cggtggcacg ctcgatgctt caccggcccg ctcgagggag gaactgctat ggatccactt
     3121 ggcctgctgg agacgatctt cgtcggggcc accgttgccg gcgccctcac agtctcggtg
     3181 ttggtgctgg gcgtgctcgg catcgccgcc cgcgggggtg tccgacacat ggaggacttt
     3241 ccgtatcccg gcgtgacggc acgtgccgag gaggtcgagt cgcgcctgcg gcatcgatac
     3301 ctgatgcgga ccggcacgct ccccgtgcgg ccggcggcat ccgaccaagc gttcaccttc
     3361 gagcccgatc ccgaaccggc gccacagccg gtgctcgtac cggcggcgtc gggcggtgga
     3421 ttcaccgccg cgctcatggt tcgggaagcg accgaaagcc cgcgccgcgg tcgacgcggc
     3481 cgtcggagtc gggggcggga caagccgaag gtcgccgcct agcaacctcg tcagccaatc
     3541 cccagccggg atcgcgtgga cccggcccgt ccgcgccgct accctccggg cgtggacgtc
     3601 atcgatctgc gctcggacac ggtcacccgt cccagcgcgg ccatgcgtcg cgccatggcc
     3661 gaggctgaag tcggtgacga ccagtacggg gaggacccat cagtcaaccg gctgcaggcg
     3721 gaggcagctg aactgctggg caaggattcc gccctgctgc tgcccagcgg aaccatggcc
     3781 aaccaggtgg cgctccggac gctcacgcat tcgggtgacg atgtgctggc cccccacgag
     3841 ccacacatca tcttccacga gaccggcgcg gcggcggcca acgcgggcgt gcagcttcgc
     3901 ttcatcggcg gtcgcgatgg gcggttcacg gccgatgagt ttgcggccgc ctgcatcccg
     3961 cgcggccatc tgaccagccc gcccaccacg ctggtggtca tcgagaacac gcacaaccga
     4021 gcgggcggga ccatctttcc cctgaccgat gtctccgcgg tctcggcggc ggctcgagac
     4081 cgtggagtgg ccacctactg cgacggtgcg cggctgctga acgccgcggt cgccaccggc
     4141 cgacctccgg cggagctcgc ggccccgttc gacctggtgg ccatctcgct ctcgaagggg
     4201 ttgggtgcgc cgttcggatc gctgctggcc gggtcgcgcg agctgatcgc gtcagctacc
     4261 cgccaccgcc ggatgctggg cggcgccctt cggcagatcg gcatcctggc cgcggccggg
     4321 tctgtggccc ttcaacacaa cctgggacga ctggttgacg accatgccgc tgcacgcgcc
     4381 atagccgacg ttctggccgg gagccccgcc atcgcgctcg aaccggggaa cgtggagacc
     4441 aacatcgtgg tcttcggcct gaccgatgcc gccggcgtgc ccgacgccgc ggcgttcgtg
     4501 gacggctgcc ggcagcgcga cgtgctgctc ttcgcattcg ggccgcggat gctgcgcgcg
     4561 gtgacgcacc tggacgtcag ccgcgagcag tgcctcgccg cggccgagcg gatggtcgcg
     4621 gtggccgagg gccgctagcc cttcttgcga cgctcgcgct cctcggcgcg acgcagctcg
     4681 cgaccggatg gctcctggtg cggcgccccc ggctcagcga gtcgttcctc gctccgcgac
     4741 tcggctccgc ggccgcgctc caggttgtgg ctgcgcggcg cgaccgtccc gcccttccag
     4801 tgatcgaccg cgccctcgtc tcgtgctcgc ttggtcatgg caccccccgc cgcacgaaac
     4861 gtgccgggcg gaggctatcc ggcgccggac ggcgggacgt tgtggttgcg gtggaagacg
     4921 ttgtctgggt cgtatcggtc cttcagggcc gtcaggcgtg ccagcgtgcg ttcccggtag
     4981 gcctgtcgca gcccgctcgc gccctcgtcc gagaggccgt tgacgtaggc gccgctggcg
     5041 aacggctgta gcgccgccgc gtagcgtcgc gaggcggtca tgcgcgcctc gtcctcggcg
     5101 gggtcctccc agccggccat ggtcacgaac tcgaacctgg cgtcgcgctg gctgaaggcg
     5161 gtgtcgtccg gccccacgtc cgcg
//
LOCUS       DATACA010000277         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2847633, whole genome shotgun sequence.
ACCESSION   DATACA010000277 DATACA010000000
VERSION     DATACA010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2847633"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..863
                     /locus_tag="VHK28_05715"
     CDS             321..863
                     /locus_tag="VHK28_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019552765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX2141760.1"
                     /translation="MEEHPKGGFHGLLEVIPTKADYVLDLIRANSLWPLLSGLSCCAI
                     EMMSTATSKNDIDRFGMFPFRASPRQADVLIVAGTLTTKMAGPLVRLWEQMPEPKWCV
                     AMGTCTTSGGRFKRSYSVVQGVDRVMPVDVYVPGCPPRPEGLIYGLLKLQELVKQRRG
                     QWPTYVDRGPQRVAWEGDVR"
     gene            803..1519
                     /locus_tag="VHK28_05720"
     CDS             803..1519
                     /locus_tag="VHK28_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2141761.1"
                     /translation="MADLRRPRPAARGLGRRRALTAPAAAGALVRALTDRFPELADTL
                     WTDEDTVELVIDGARNVEVLQAVRDDPELRFWWLGDAAAVDYGPRGSDPHFEVVYHLY
                     SDQHPAWLRIRARVDRANPHIPTITELWEGAMFHEREMYDMMGIVFDGNRDLRRIFMS
                     PDYRSFPQRRDFILPDDAADSAAAGVNPLERPETWDLSAFRADLTDSTAATYAPTAER
                     TGQEPEAEGGEALADKSRPR"
     gene            1516..>2666
                     /locus_tag="VHK28_05725"
     CDS             1516..>2666
                     /locus_tag="VHK28_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141762.1"
                     /translation="MTKIDPETRIPDPIELDRAEEQRLAAALGARPGAAPQREAQSSG
                     RPEFAGFETVGLPDRPPQTEREREMYTLEEGEMLLNMGPQHPSTHGVMRLVVKIRGEV
                     VTDLDPVLGYLHRGIEKLCENATYTTVLTYVDPMDYLSSMHNEHAPAIAFEKLYNIKA
                     PPRAEYIRALVVELNRVFSHGLMMGFLALDMGGLTPILYGFINRDEVVDLLASISGQR
                     MLFNFFRVGGVNWDVNDEFISRLSTWRSNVGKSVGDNEALITENEIFVQRTKGMGVLS
                     GERAIALGVSGPNLRASGVAFDVRKAHPYSIYDELEFDVITQPDGDSYARYLQRVAEI
                     KQSLHRSMRHLVHDLVDQVQALLDLGHPLQVARVAVAVRLGDHVELELVV"
BASE COUNT          444 a          944 c          886 g          392 t
ORIGIN      
        1 gtgcgcgcgg tggagctgcc cgggatcatc gccattggcg tgttccttgc gatcctgatg
       61 ttcggtgagc tctacgcctg gcggaaggga gacctgcgat ggcactagac gaccgaaccc
      121 cgcggcgacg ccggcccagc aaggcggcgc ccgcgcctga agcaaccgat gcggccgagg
      181 cacagaccga acagatggcg ggtccggtgg gcaacacgct ggccaccgat ggcgacaacg
      241 gcgggccgga tggcaacggc ggcacgccga tcatcatccc ggaccaggcc gcggacgacg
      301 acctgctccg ccagttcggc atggaggagc atccgaaggg tggcttccat ggcctgctgg
      361 aggtgatccc caccaaggcg gactacgtcc tggacctgat ccgggccaac tcgctgtggc
      421 cgctgctgtc cgggctgtcg tgctgcgcca tcgagatgat gagcaccgca acgtccaaga
      481 acgacatcga ccgcttcggg atgttcccct tccgcgcctc gccgcgacag gcggacgtgt
      541 tgatcgtggc cggcaccctg accaccaaga tggccgggcc gctggtccgc ctctgggagc
      601 agatgcccga accgaagtgg tgcgtggcca tgggcacctg caccacctcc ggcggccgct
      661 tcaagcgcag ctacagcgtg gtgcagggcg tcgatcgggt catgccggtg gacgtctacg
      721 tgcccggctg cccgccgcgc cccgaaggcc tgatctacgg gctgctcaag ctccaggagc
      781 tggtcaagca gcgccgcgga cagtggccga cctacgtcga ccgcggcccg cagcgcgtgg
      841 cctgggaagg agacgtgcgc tgaccgcacc ggccgcggcg ggagcgctgg tacgcgcgct
      901 caccgaccgc tttcccgagc tggccgacac cctgtggacc gatgaggaca ccgtggagct
      961 ggtgatcgac ggcgcccgca acgtggaggt gctgcaggcc gtccgcgacg atccggagct
     1021 gcgcttctgg tggctgggcg atgccgccgc ggtcgactac ggcccgcgcg gctcggaccc
     1081 gcacttcgag gtcgtgtacc acctctactc cgaccagcac ccggcctggc tgcgcatccg
     1141 cgcccgggtg gaccgcgcca acccccacat cccgaccatc accgagctgt gggagggcgc
     1201 catgttccat gagcgcgaga tgtacgacat gatgggcatc gtcttcgacg gcaaccgcga
     1261 cctgcggcgc atcttcatgt cgcccgacta ccgcagcttt ccgcagcgca gggacttcat
     1321 cctgcccgat gacgccgccg actcggcggc tgccggcgtc aaccccctgg agcgccccga
     1381 gacgtgggac ctctcagcct tccgtgccga cctgaccgat tccaccgccg cgacctacgc
     1441 ccccaccgcc gagcgcaccg gacaggagcc cgaggcagag ggcggcgagg cactggccga
     1501 caagagccga ccacgatgac caagatcgat cccgaaaccc gtattcccga tcccatcgag
     1561 ctcgaccgcg ccgaggagca gcgcctggcg gccgcgctcg gcgcacgacc cggcgccgcc
     1621 ccgcagcgcg aggcccagtc ctccgggcgc cccgagttcg ccggcttcga gacggtcggc
     1681 ctgccggacc ggcccccgca gaccgagcgc gagcgcgaga tgtacaccct cgaagagggg
     1741 gagatgctgc tcaacatggg gccgcagcac ccctccaccc acggcgtcat gcgcctggtg
     1801 gtcaagatcc gcggcgaggt ggtgaccgac ctcgacccgg tgctgggcta cctgcaccgc
     1861 ggcatcgaga agctgtgcga gaacgcgacc tacaccacgg tgctcaccta cgtggatccc
     1921 atggattacc tgtcgtccat gcacaacgag cacgcgccgg ccatcgcctt cgagaagctg
     1981 tacaacatca aggccccgcc gcgcgctgag tacatccgcg ccctggtggt ggagctgaac
     2041 cgcgtcttca gccacgggct gatgatgggc ttcctggcgc tggacatggg cggcctgacc
     2101 ccgatcctgt acggcttcat caaccgggac gaggtggtgg acctgctggc ctccatctcc
     2161 gggcagcgca tgctcttcaa cttcttccgg gtgggcggcg tcaactggga cgtcaacgac
     2221 gagttcatca gccggctgtc gacctggcgc tcgaacgtgg gcaagagcgt cggcgacaac
     2281 gaggcgctga tcaccgagaa cgagatcttc gtgcagcgca caaaggggat gggcgtgctc
     2341 tccggcgagc gggcgatcgc gctgggcgtc tcgggaccca acctgcgggc ctccggcgtg
     2401 gccttcgacg tccgcaaggc gcacccctac tcgatctacg acgagctcga gttcgacgtg
     2461 atcacccagc cggacggcga cagctacgcg cgctacctgc agcgagtggc cgagatcaag
     2521 cagagcctgc accggtccat gcggcatctt gtccacgatc tggtcgatca ggtgcaggct
     2581 ctgcttgatc taggccaccc gctgcaggta gcgcgcgtag ctgtcgccgt ccggctgggt
     2641 gatcacgtcg aactcgagct cgtcgt
//
LOCUS       DATACA010000278         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2686634, whole genome shotgun sequence.
ACCESSION   DATACA010000278 DATACA010000000
VERSION     DATACA010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686634"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VHK28_05730"
     CDS             <1..494
                     /locus_tag="VHK28_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2141763.1"
                     /translation="DQPDDIIAFPTPAGPEGTAFMPVVAGLGIPTWAPNPDAARELIR
                     HLLQPQTQATTLSEVAFFPVISAELPTDLEAGVQAEQEAVARQTGRDDALPSLLPIGL
                     GDQGNAYNQVFRDVFQAIVLNGDDPAAVLESQLPNLQAVFDASGAACWPPDPPSDGPC
                     EAQ"
     gene            575..1432
                     /locus_tag="VHK28_05735"
     CDS             575..1432
                     /locus_tag="VHK28_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2141764.1"
                     /translation="MKAGRLGWAPYALLLPSLAYLALFFAAPMIGAFGLAFQDGGGNW
                     TLASIERMLNDFNFTRALTTTLLLVILVLPVQLILAMGMALVINARLKGTGLWLYVYA
                     LPLGISELAAGIVWVSIFTQTGWLNSILQTIGIIDRPIIWQSAESQWLLILLVVIAES
                     WRATSIIMIILVAGLQSIPPEYLEAAEVYGATTWQKVRRVILPMLRPALQVALILRTI
                     LAFQVFATVVALAGTGTVVLAQEARRWAVVIDNDNVAAAYAGLILLLSIASTLIFLWV
                     LPTRDEERV"
     gene            1468..2283
                     /locus_tag="VHK28_05740"
     CDS             1468..2283
                     /locus_tag="VHK28_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2141765.1"
                     /translation="MRRRQLGRALTYLAATLLAIFVLAPIYFIAISALTPPEIALTEY
                     PRRLFPAEISFETISFFLGASGVIDSFWRSVWVGLITLVLSLLIGAPAGYAIARYAFR
                     GRETYRLVVLSTRAFPVVILAIPLAVTYIRLGINDSIIGVALMHTALALPFAILITSS
                     VFVSVPRELEEAAQTLGCHPLQAFLRVSLPLALPGLAAAAIFTWVISWNDVFAASILT
                     LREPTLPARVLTSLTQSRITFQFAAGFIMLIPSIVVIFFIRRYLLGLWGRVVK"
     gene            2299..>2423
                     /locus_tag="VHK28_05745"
     CDS             2299..>2423
                     /locus_tag="VHK28_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141766.1"
                     /translation="MAEIRLEGVTKLFKQVRAVDDVSLTIAEGEFMVLLGPSGCG"
BASE COUNT          339 a          880 c          783 g          421 t
ORIGIN      
        1 aggaccagcc ggatgacatc atcgccttcc cgacgccggc cggcccggag ggcaccgcct
       61 tcatgccggt cgtggccggg ctggggattc ccacctgggc acccaacccc gacgcggctc
      121 gcgagctcat caggcacctg ctccaacccc agacgcaggc gaccacgctg agcgaggtgg
      181 ccttcttccc ggtcatctcg gccgagctgc ccaccgacct ggaggccggg gtgcaggccg
      241 aacaggaggc ggtggccaga cagaccggcc gcgacgacgc cctcccgtcg ctcctgccaa
      301 tcgggctggg cgaccagggc aacgcctaca accaggtgtt ccgggacgtc ttccaggcca
      361 tcgtgctgaa cggggacgac ccggctgccg tgctggagag ccagctgcca aacctgcagg
      421 cggtgttcga cgccagcggg gcggcctgct ggccaccgga tccgccgagt gacgggccct
      481 gcgaggcgca gtagccaccg aggcatgacc gatgaggggc gggggcaacc cgcccccatc
      541 ggttcaccga acccgtgatc cacgaacgcg tgccgtgaag gcgggacgcc tgggctgggc
      601 gccgtacgcg ctcctgctgc cctcgctcgc ctacctggcg ctcttcttcg ccgcgccgat
      661 gatcggcgcc ttcgggctcg ccttccagga cggtggcggc aactggacgc tggcgtccat
      721 cgagcggatg ctcaacgact tcaacttcac cagggcactg accaccacgc tgctgctggt
      781 catcctggtg ctgccggtcc agctcatcct ggccatgggc atggctctgg tcatcaacgc
      841 gcgcctgaag ggcaccgggc tgtggctgta cgtctatgcg ctgccgctgg ggatcagcga
      901 gctggcggcc ggcatcgtgt gggtcagcat cttcacgcag accgggtggc tgaactcgat
      961 cctgcagacc atcggcatca tcgatcggcc catcatctgg cagagcgcgg agtcgcagtg
     1021 gctgctcatc ctgctggtgg tcatcgccga gtcgtggcgc gccaccagca tcatcatgat
     1081 catcctggtc gccggcctgc agtccatccc gccggagtac ctcgaggcgg ctgaggtgta
     1141 cggggccacc acctggcaga aggtgcgccg cgtcatcctg cccatgctgc ggcctgcgct
     1201 gcaggtggcg ctcatcctgc gcaccatcct ggccttccag gtcttcgcca cggtggttgc
     1261 cctggccggc accggcacgg tggtgctggc ccaggaggca cggcgctggg cggtggtcat
     1321 tgacaacgac aacgtggcgg ccgcctacgc cggactgatc ctgctgctct cgatcgccag
     1381 tacgctcatc ttcctgtggg tgctgcccac ccgcgacgag gaacgggtat gaccgcgaac
     1441 gcagcgccgc gccccaagcg gccaggcatg aggcggcgcc agctcggccg tgcgctcacc
     1501 tacctggccg ccaccctgct cgccatcttc gtgctggcgc ccatctactt catcgccatc
     1561 agcgcgctga cgccccccga gatcgcgctg accgagtacc cgcggcggct gttcccagcc
     1621 gagatctcct tcgagaccat cagcttcttc ctcggagcga gcggggtgat tgactccttc
     1681 tggcgcagcg tgtgggtggg cctcatcacc ctggtgctct ccctcctgat cggcgcaccc
     1741 gcggggtacg ccatcgcccg ctatgccttc cgcggccgcg agacgtaccg cctggtggtc
     1801 ctctcaaccc gtgccttccc ggtcgtgatc ctggccatcc cgctggcggt gacctacatc
     1861 cgcctgggca tcaacgactc gatcatcggc gtggcgctga tgcacaccgc gctggcgctg
     1921 ccgttcgcca tcctgatcac cagcagcgtg ttcgtatcgg tccctcgaga gctggaggag
     1981 gcggcgcaga cgctggggtg ccatccgctg caggccttcc tgcgggtgtc cctgcccctg
     2041 gcgctccccg gcctggcggc tgcggccatc ttcacctggg tcatcagctg gaacgacgtc
     2101 ttcgccgcca gcatcctcac cctgcgcgag cccaccctgc cggcccgggt cctgacctcg
     2161 ctgacgcagt cgcgcatcac cttccagttc gcggccgggt tcatcatgct gatcccgtcg
     2221 atcgtggtca tcttcttcat ccgccgctac ctgctcggcc tgtggggccg ggtcgtgaag
     2281 tgaggaggtc cgccgcccgt ggctgagatt cgcctggagg gtgtcaccaa gctgttcaag
     2341 caggtgcgcg ccgtcgacga cgtcagcctg accatcgccg agggcgagtt catggtgctg
     2401 ctcggaccgt ccggctgcgg gaa
//
LOCUS       DATACA010000279         1531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2445616, whole genome shotgun sequence.
ACCESSION   DATACA010000279 DATACA010000000
VERSION     DATACA010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445616"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHK28_05750"
     CDS             <1..307
                     /locus_tag="VHK28_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2141767.1"
                     /translation="HAIAARVIDLRWLNPIAFDAVRAAAAETGSVLVVDECRHTGGGV
                     ADAIIADLAEQGIGRRLGSVRSVDSFVPLGPASAAVLIGVDQVIAGALAAVGERTLS"
     gene            388..1347
                     /locus_tag="VHK28_05755"
     CDS             388..1347
                     /locus_tag="VHK28_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX2141768.1"
                     /translation="MPTDDPLVQRAEELRAGYGLEPLLALDGAERFDPARHLRPANAS
                     PLTLLATMLDVEQALADHQVAAVIGNSLGWYTALAVTGALPFDDAFRLVQETALLQEE
                     PLPAGGPGGQVIYPLVDAEWRADPELGAAVSGALADTVDGEAGRGRAYQSIDLGGYAV
                     LAGDEAGVASLLRSLPPVRSGERVFPLRLALHGPYHTPLVEHVSTAARERLGGLAWQA
                     PAWTLVDGRGVRWTPWSTDPDALRDYTLGEQVVTPYRFASSLRVALREYAPDVVVLPG
                     PGNSLGGICGQLVVAEGYRAIRGRADFDGIQRSDRPVLLSMRR"
BASE COUNT          189 a          552 c          570 g          220 t
ORIGIN      
        1 gcacgcaatc gcggcgcgcg tcatcgacct gcgctggctg aatccaatcg ccttcgacgc
       61 ggtccgcgcc gccgccgcgg agaccggcag cgtgctggtg gtcgacgagt gccgtcacac
      121 cggcggcggg gtggcggacg cgataatcgc cgacttggcc gagcagggca tcggccggcg
      181 gctgggatcg gtgcgttccg tggacagctt cgtcccgctg ggaccggcga gcgcggccgt
      241 gctgatcggc gtcgaccagg tgatcgccgg cgcgctggcg gccgtgggcg agcgaacgct
      301 gtcatgagtg cggagcacga gggcttgccg cgggcggtct tcgtctgccc ggggcggggc
      361 tcctataccg cgacgtcact cggcagcttg ccaaccgacg acccgctcgt gcagcgtgcc
      421 gaggagttgc gcgccggcta cgggctcgag ccgctgctgg cgctggacgg cgccgagcgc
      481 ttcgatccgg ctcgccacct gcgacccgct aacgcgtcgc cgttgaccct gctggcgacc
      541 atgttggacg tcgagcaggc gctggccgat caccaggtcg ccgctgtcat cggcaactcg
      601 ctgggctggt acaccgcatt ggccgtcacc ggggcgctgc ccttcgatga cgcgttccgc
      661 ctcgtccagg agacggcgct cctccaggag gagccgctgc cggcgggcgg tccgggcggc
      721 caggtcatct acccgctggt ggacgccgag tggcgcgccg acccggagct gggtgcggcc
      781 gtgagtggcg cgctggccga caccgtggac ggcgaggcgg gccgcggccg cgcctaccag
      841 agcatcgacc tgggcggcta cgcggtcctg gccggcgacg aggcgggagt cgccagcctg
      901 ctgcgcagcc tgccgccggt gcgcagcggt gagcgcgtat tcccgctccg cctggcgctc
      961 cacgggccgt accacacgcc gctggtcgag cacgtgtcga cggcggcccg ggagcgcctg
     1021 ggcggcctgg cctggcaggc gccagcctgg acgctggtgg acggccgcgg cgtgcgctgg
     1081 acgccgtggt caaccgatcc ggacgcgttg cgcgactaca ccctgggcga acaggtggta
     1141 acgccgtacc gcttcgcgag cagcctgcgg gtcgcgctcc gcgagtacgc ccctgacgtg
     1201 gtcgtccttc ccgggccggg caactcgctg ggtggcatct gcggtcaact ggtggtggcg
     1261 gagggatacc gggccatccg cggccgcgcg gacttcgacg gcatccagcg ctcggatcgg
     1321 ccggtcctcc tctccatgcg ccgatgagga tgccccgccc gatgctggcg ccgcccgctg
     1381 caccggatgc atccaccatc ctgccgctgc ctgcggcatt cgacctgcgc ctgaccatgg
     1441 cgccgctggc ccacggcacc ggagatccaa ccatccgtat cgagcgacag gcagtgtggc
     1501 gcgtcacgcg cactcgacgc gggccagcca c
//
LOCUS       DATACA010000280         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2848029, whole genome shotgun sequence.
ACCESSION   DATACA010000280 DATACA010000000
VERSION     DATACA010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848029"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VHK28_05760"
     CDS             <1..536
                     /locus_tag="VHK28_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEX2141769.1"
                     /translation="FFFPGGETVLVRIGPVVATAEGLGLALEILVRIMAISGAITLFY
                     LTTRPADLVVDLERRGVSQRVAFVAHASVQTVPAMVERAAQITDAQRARGLDTEGSVW
                     RRVRGLVPIVGPVILGSIAEVEERTMALEARGFSRPGRRTLLWTPPDSRRQGLLRWGM
                     LAALAALIVLRVTGILP"
     gene            533..1354
                     /locus_tag="VHK28_05765"
     CDS             533..1354
                     /locus_tag="VHK28_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141770.1"
                     /translation="MTLRLAGVGYRYAGAREPSLLDVDLELPDGTVLGVAGASEAGKT
                     TLCLVASGLAPRVVGGRIRGHITLDGEDVDPWPMHRMAGQVAIGFQNPATQLSGVAST
                     VFEEVAFGPMNLAVPLPDVAARTWKALAALRIEGLAERDPARLSGGQQQLVAIAGLLA
                     MRPRHLVLDEPTAQLDPAGTRLVADALAALAGAGTSILITEQKTDLLAAICDRVVVLS
                     EGRVAFAGDADRVLSDPRLVELGIAPPAAIRIRRAARAAGLAAAELSRLVAALDR"
     gene            1351..2208
                     /locus_tag="VHK28_05770"
     CDS             1351..2208
                     /locus_tag="VHK28_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141771.1"
                     /translation="MTTAARPPTIRLERVSYVYPRSEVRALDEVELEIAPGDVVALVG
                     QNGSGKSTLVRHLNGLLRPTRGEVSFDGDSTRRRTVAELARIVGLVFQDPDRQIFAGS
                     VRAEVEFGPRNVGLSGATLRAAVDEALEAIGLADEAGTNPYDLGGSRRKLLALASVLA
                     MRTPVLVLDEPTTGQDLRGVATVRRVIDQAAAAGRTVIAISHDMEFVAEAFARVVVLR
                     EGRLILDGPPASVFAQDRWDSLASTYLEAPLAARLGARLRLDHVTPTDAALLAALASL
                     GAAGPAGDQ"
BASE COUNT          275 a          867 c          878 g          332 t
ORIGIN      
        1 tgttcttctt ccccggcggg gagacggtcc tcgtgcgcat cggtccggtg gtggccaccg
       61 cggagggcct cggcctggcg ctggagatcc tggtgcggat catggccatc agcggcgcca
      121 tcaccctctt ctacctgacc acccggccgg ccgacctggt ggtggacctc gagcggcgcg
      181 gggtcagcca gcgggtggcc ttcgtggccc acgcatcggt ccagaccgtg ccggccatgg
      241 tcgagcgcgc cgcgcagatc accgacgccc agcgcgcgcg aggcctggac accgagggca
      301 gcgtctggcg ccgggtgcgg gggctggtgc cgatcgtggg accggtcatc ctgggctcca
      361 tcgccgaggt cgaggagcgg acgatggccc tcgaggcgcg cggcttctcc cgccccggcc
      421 ggcgcacgct gctgtggacg ccgccggatt cccgacgcca ggggctcctg cgctggggga
      481 tgctggccgc actggccgcg ctgatcgtgc tgcgcgtgac gggcatcctg ccgtgacgct
      541 gcggctggcc ggcgtcggct atcgctacgc cggggcgcgc gagccgtcgc tgctggacgt
      601 cgacctcgag ctgccggacg gcacggtgct gggcgtggcc ggggcatcgg aggcgggcaa
      661 gaccacgctc tgcctggtcg cctccgggct ggcgccgcgc gtggttggcg ggcgcatccg
      721 cggccacatc accctcgatg gcgaggacgt cgatccctgg cccatgcacc gcatggccgg
      781 gcaggtggcc atcggcttcc agaacccggc cacccagctg tccggggtgg cctccaccgt
      841 gttcgaggag gtcgccttcg gaccgatgaa cctggcggtg ccactccccg atgtcgcggc
      901 acgcacgtgg aaagcgctgg ccgcgctgcg catcgaaggg ctggcggagc gcgacccggc
      961 ccggctctcc ggtgggcagc agcagctggt ggcaatcgcc ggcctgctgg ccatgcggcc
     1021 gcgccacctg gtgctggacg agccgacggc ccagctcgac ccggccggga cccgactggt
     1081 ggccgatgcg ttggccgccc ttgccggcgc cggcacctcc atcctgatca ccgagcagaa
     1141 gaccgatctg ctggccgcca tctgcgaccg cgtcgtcgtg ctctccgaag gtcgcgtggc
     1201 gttcgccggc gacgcggacc gggtgctctc cgatccgcgg ctcgtcgagc tggggatcgc
     1261 gcccccggcc gccatccgca tccggcgagc ggcacgggcc gcgggcttgg ccgcagccga
     1321 gctctcgcgg ctggtcgcgg cgctggaccg atgaccacgg ccgcacgtcc gcccaccatc
     1381 cgcctcgagc gggtgtccta cgtctacccg cgcagcgagg tgcgcgcgct cgacgaggtg
     1441 gagctggaga tcgccccggg cgatgtcgtg gccctggtcg gccagaacgg cagcggcaag
     1501 tcgacgctgg tgcgccacct caacggcctc ctgcggccca cccggggcga ggtcagcttc
     1561 gacggcgact cgacccggcg ccgcacggtg gccgagctgg cgcgcatcgt cggcctcgtc
     1621 ttccaggacc cggaccggca gatcttcgcc ggcagcgtgc gcgccgaggt cgagttcggc
     1681 ccgcgcaacg tcggcctctc gggggcgacg ctgcgggccg ccgtggacga ggccctggag
     1741 gccatcggcc tggccgatga agcgggcacc aacccctacg acctgggcgg ctcgcggcgc
     1801 aagctgctgg cgctggcatc ggtcctcgcc atgcgcaccc cggtgctggt gctggacgag
     1861 ccgaccaccg ggcaggacct gcgcggggtg gccaccgtgc gccgggtgat cgaccaggcg
     1921 gccgcggccg gccggacggt catcgccatc agccacgaca tggagttcgt ggccgaggcg
     1981 ttcgcccgcg tcgtcgtcct gcgcgagggg cggctcatcc tcgacgggcc gcccgcatcg
     2041 gtctttgcgc aggaccgatg ggattcactg gcctcgacct acctcgaggc accactggcc
     2101 gcccgcctgg gtgcgcgcct gaggctcgac cacgtcacgc cgaccgatgc cgccctgctc
     2161 gccgcgctcg cctcgctcgg cgcagctggc ccagcgggcg atcagtagcc ggtgtagaag
     2221 cccggagggg gtggcaggag gttgccctcg gcatcggtcc gctcgtcggg cggaagctcg
     2281 tcgcgatgcc gccgctcccc cgtcggcccc gggatgggca gggccatgac cgagctgggg
     2341 acctcgttca gc
//
LOCUS       DATACA010000281         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1642357, whole genome shotgun sequence.
ACCESSION   DATACA010000281 DATACA010000000
VERSION     DATACA010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642357"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1727)
                     /locus_tag="VHK28_05775"
     CDS             complement(168..1727)
                     /locus_tag="VHK28_05775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2141772.1"
                     /translation="MTTALPLRSYTPLRMVLTVCGHCFSEDPDREVDYGTDILQGNLV
                     MQDGSVYLRRYCRRGHGEVVSLYDEDYPLWEYLQQWRVPTREIVEDTPDNVMPIPMGY
                     ANGLGRLQTQHSCILLLDVTEHCNLNCPTCFAASGTGVGRYARLPHILRTLDASIERE
                     GGKIDVLMLSGGEPTVHPEIVEIIQAAVERKVTRVLLNTNGIRVARDDRFLAALQKLR
                     GSVEVYLQFDGFEKETHLFHRGEDLREVKAEAIRRLTDARIFTTLTMVVAQGINDHEM
                     GAVANFAFDTDYIAGVMYQPVFGSGRSNPIDPMQRMTTTGVLKRLGPQTDGRAAAEDF
                     IALPCSHPDCCAITYFVREDSGTYRSIPKLVGIDRLKDNLSIVGNRIAADDELWDALI
                     GMMSETTTISRPELIDYLINICEACDIGVGGFVKDLGRYLVKGERAAELVALRTKRLS
                     VKSFMDAWTMNIERLQQCCVHVGSVDGEADPVRIPFCARQLFGGLRRRTSVGQVGARE
                     LVQLDGLGARA"
     gene            complement(1738..2331)
                     /locus_tag="VHK28_05780"
     CDS             complement(1738..2331)
                     /locus_tag="VHK28_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141773.1"
                     /translation="MSTEHHAIDEYIRANRDRYTREAITQQLREAGHAPEDIGAAWDR
                     ADGVGPTDGPAWRPGLTTFLVLAALGAVGAALVWMDNPYGGSGLAVMIYLAILLPTYG
                     IAKGASVLIDRGRGWVAGILLMLIALAGVALGFSNASPFIALLVVVAAGIPALALVSS
                     RLGSQWAGWISAALPILVWLGITGTCYAPLLDSIGSG"
BASE COUNT          392 a          843 c          818 g          395 t
ORIGIN      
        1 cgccacggcc aggacgaaga tcgggggcgt gaccagccac gcgatcagac ccaccgtccc
       61 catcttgcag aaggtgaacg ccaggcggtc gtacttctgc gcccgcgtca tcggcgggcg
      121 gctggccagc caggccttca tggggtcgac agggccgacg tcggcgctca tgcccgcgct
      181 cccagcccgt ccagctgcac cagctcgcgg gcgcccacct ggcccaccga cgtccggcgt
      241 cgcagcccgc cgaacagctg gcgcgcgcag aacgggatgc gcaccgggtc ggcctcgccg
      301 tcaaccgatc cgacgtgcac gcagcactgc tggagccgct cgatgttcat ggtccaggcg
      361 tccatgaacg acttgacgct cagccgcttc gtgcgcagcg ccaccagctc ggccgcccgc
      421 tcccccttca ccaggtagcg acccaggtcc ttgacgaacc cgcccacccc gatatcgcac
      481 gcctcgcaga tgttgatgag gtagtcgatc agctcgggcc ggctgatggt ggtcgtctcg
      541 ctcatcatcc cgatcagcgc gtcccacagc tcgtcgtcgg cggcgatgcg gttgcccacg
      601 atcgacaggt tgtccttgag acggtcgatt cccaccagct ttggaatgga ccgatacgtc
      661 ccactgtcct ccctcacgaa gtaggtgatg gcgcagcagt ccggatggct gcagggcagc
      721 gcgatgaaat cttccgcggc tgcccgccca tcggtctgtg ggccaaggcg tttcaacacg
      781 ccggtggtgg tcatgcgctg catggggtcg atggggttcg agcggccgga gccgaatacc
      841 ggctggtaca tcacgccggc gatgtaatcg gtgtcgaagg cgaagttggc caccgccccc
      901 atctcgtggt cgttgatgcc ctgggccacc accatggtca gcgtggtgaa gatccgggca
      961 tcggtcaggc gccgaatggc ctcggccttc acctcgcgca ggtcctcacc gcggtggaag
     1021 aggtgggtct ctttctcgaa gccgtcgaac tggaggtaga cctccacgct gccgcgcagc
     1081 ttctggaggg ccgccaggaa gcgatcgtcg cgcgccacgc ggatgccatt ggtgttgagc
     1141 agcacccgcg tcaccttgcg ctccaccgca gcctggatga tctcgacgat ctccgggtgc
     1201 accgtcggct cgccgccgga gagcatcagt acgtcgatct tgccgccctc gcgctcgatg
     1261 ctggcgtcca gggtgcgcag gatgtgcggc aggcgtgcgt agcggcccac cccggtgcct
     1321 gacgcggcga agcaggtggg acagttcagg ttgcagtgct cggtgacgtc cagcagcagg
     1381 atgcaggagt gctgggtctg taaccgccca aggccgttgg cgtagcccat ggggatgggc
     1441 atcacgttgt ccggcgtgtc ctccacgatc tcgcgggtcg ggacgcgcca ctgctgaagg
     1501 tactcccaca gcgggtagtc ctcgtcgtag aggctcacca cctcgccgtg tccccggcgg
     1561 cagtagcggc gcaggtagac cgatccgtcc tgcatgacca ggttgccctg caggatgtcg
     1621 gtcccgtagt cgacctcgcg gtccgggtcc tcgctgaagc agtggccgca caccgtcagg
     1681 accatgcgca gcggtgtgta ggaacgaagc ggaagcgcgg ttgtcacacg atcctcctca
     1741 gccggagccg atgctgtcga gcagcggcgc gtaacaggtc ccggtgatgc cgagccagac
     1801 caggatcggc agggccgcgc tgatccaccc cgcccactgc gaacccaggc gagacgacac
     1861 cagggccagc gccggaatgc ccgcggccac gaccaccagt agggcgatga acggcgaggc
     1921 attgctgaag ccgagcgcca ctccggccag cgcaatgagc atcaggagga tgccggcgac
     1981 ccagccgcga ccgcggtcga tcagcacgga ggcgcccttg gcgattccgt aggtcggcag
     2041 caggatggcc aggtagatca tgaccgccag gccgctgccg ccataggggt tgtccatcca
     2101 gaccagggcc gcacccacgg cgccgagggc ggccagcacc aggaacgtcg tcagccccgg
     2161 tcgccaggcg gggccgtccg tcgggcctac accgtcggcc cggtcccagg cggcgccgat
     2221 gtcctccggc gcgtgtccgg cctcccgcag ctgctgggtg atcgcctcgc gggtgtatcg
     2281 gtcccggttg gcgcggatgt attcgtcaat ggcgtggtgc tcggtgctca ttggcagctc
     2341 cagctgaacg ttccagtgat ggcagatccg aagtcttccg gaccggggtt cgccggtccc
     2401 ccctcctcga ggcgcaggcc atcgaaggta atcatccccc tgccgccg
//
LOCUS       DATACA010000282         8457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1000393, whole genome shotgun sequence.
ACCESSION   DATACA010000282 DATACA010000000
VERSION     DATACA010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000393"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VHK28_05785"
     CDS             complement(<1..1478)
                     /locus_tag="VHK28_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2141774.1"
                     /translation="MADLNEGRLDLPRSRARFVAFGLAAALLFTALGGRLFQLQVVNG
                     EQFAAQARATRSVEVPLRSVRGLVFDREGRPVAVNVPSWTVKVRPADLPERRESGILL
                     RVAQITGSDPRDLRRRLDAYSGSPFDMVPVERGISREAALLINEESENLPGVVVEVDP
                     IRQYLNEKGEVDGALLSHILGYVGPVDLPEFRELADDGYLRDDVIGRAGIEATFEEQL
                     RGVYGAELAERDAAGRLGKTLETVSEPEPGKNLALTIDARMQRIATDALRWGMGAVGV
                     EQGVTVVMNPQNGEILAMVSLPAYDNNKFATGISAQDYSVYLEDPAKPLRNHAIADIF
                     PPGSTFKLVTGLAALQEGITSAARRWPTYGCYQIPGAPAGQCLFDWNRQGFGPLDMID
                     AFAVSSDTYYYQMAIALGVDALGAWANELGFGERTGVSLPGEAAGIIASRAWARSQGR
                     ENLFTGELAQAGIGQNVVAVTPIQLLNAYAALANGGTLWRPQ"
     gene            complement(1489..1986)
                     /gene="mreD"
                     /locus_tag="VHK28_05790"
     CDS             complement(1489..1986)
                     /gene="mreD"
                     /locus_tag="VHK28_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX2141775.1"
                     /translation="MSYTSVLLAVVVGIVHAGLSPVIEIAGVRPNLVLVAVVLVTSLL
                     GFGPGIVWAFAAGLTANLLVPDPLGSLPLSMLLVAAAVAGGSRVLGRLVWLYPVLAAF
                     AGSVLADAVTLGVVGLVDEPLRVPAPLQLILPAATLNAAIAGLLLYPARSAAARVLPE
                     ERPAW"
     gene            complement(2059..2388)
                     /locus_tag="VHK28_05795"
     CDS             complement(2059..2388)
                     /locus_tag="VHK28_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141776.1"
                     /translation="MERTGTEFDLGMLVGLLVGEGHFGGDGRVPQITLRMHVRHEAMF
                     RWLVERWGGRLYGPYSHGGRDYYQWMARGAYLRDELVPLLDRVMGPELDGHAWTRYRD
                     MKDRYGL"
     gene            2547..3308
                     /locus_tag="VHK28_05800"
     CDS             2547..3308
                     /locus_tag="VHK28_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007409899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141777.1"
                     /translation="MVRVTACTNQKGGVGKTTTVVNLAAFLALSGTSTLVIDLDPQGN
                     ATSGLGVDRRSLDRSVYDAIVDRTPIADLIVGTAVDGLDLLPSASALSGAEVELVQVP
                     ARERRLEASLAGLDGTYDRVLIDCPPSLGLLTVNALTAADGVLIPIQTEYYALEGLSQ
                     LVNTIRRVREALNPRLEIEGVLLTMYDARTNLSAQVAAEVRRHMNGTVFDTVVPRSVR
                     LSEAPSHGLPIALYDPASRGAHAYGQLAAEVDARG"
     gene            3301..4191
                     /locus_tag="VHK28_05805"
     CDS             3301..4191
                     /locus_tag="VHK28_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX2141778.1"
                     /translation="MARPFGLGRGLDALIPRSAESGSGSLQIPVERVHRNPHQPRTAF
                     GDEGLAELAASIAAHGVLQPIVVRGAADGGYELIAGERRLRAARMAGLTEIPAMVRDS
                     QPSELLELALVENVQREDLNPIEQATAYRELIDRFGLSHEAVARQVGKSRVAVSNALR
                     LLDLAPETRQAIIDGRITEGHGRALAALTIPELQRAVLQVVLERGLSVRQTEELVRRK
                     RETARPPTRQALSEDLADVEAQLRGILATKVGIVRTRRGGRVVIDFYSDEELDRIYAI
                     ISRGAGSPSAAHEERREDRA"
     gene            4188..6164
                     /gene="gyrB"
                     /locus_tag="VHK28_05810"
     CDS             4188..6164
                     /gene="gyrB"
                     /locus_tag="VHK28_05810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX2141779.1"
                     /translation="MTVEPARRTRRKADGYTAADIQVLEGLQAVRKRPGMYIGSTDTR
                     GLHQLVWEVVDNSIDEAMANVATRIDVTIHADNSMEVVDDGRGIPVGKHSSGKDALEV
                     VHTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSTHMRVEVLRDGKRWVQEYADGGKP
                     RGPVKQTGGKAEANRGLDPDWKRQHGTRTFFTPDASIFETLDYSWETIVTRLRESAYL
                     NKGVWIRLVDERTDREKNFYFEGGVTSFVRHLNRRRDVLNPKPIHVERVVDTTSVEVA
                     LQYNDGFAETVLAFANNIHTVDGGSHVAGFRAALTGSLNDWARKSSTLSEKEANLSGE
                     DVREGLTAVISVKLTDPQFEGQTKAKLGNADVKGIVQRVVTEGIIQHLEENPADGRRI
                     IEKSLTAARAREAARKARDLVIRKGALDGMALPGKLADCQERDPARSELYIVEGDSAG
                     GSAKQGRDRRFQAVLPLRGKLLNVEKARLDRVLSSENIRPLIIALGAGIGDQFDIAKL
                     RYHRICLLSDADVDGAHISTLLLTFFFRHMPEVIDNGYLYLCQPPLYRVSTGKVTRYA
                     STEKERDQAMKELSREGRTKNITVQRFKGLGEMNADQLWETTMNPETRTLLQVSVGDA
                     AEADEIFSTLMGERVEPRRDFIRGEARKVRNLDF"
     gene            6193..6441
                     /locus_tag="VHK28_05815"
     CDS             6193..6441
                     /locus_tag="VHK28_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEX2141780.1"
                     /translation="MPSRHVADIRQVPAKTGRNVEEWCELLEANGVGDRRDAVNFLRD
                     GYAISHAFALAIADHCLRRSRDDDFEDPWAGDDEEPAW"
     gene            6438..6980
                     /locus_tag="VHK28_05820"
     CDS             6438..6980
                     /locus_tag="VHK28_05820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX2141781.1"
                     /translation="MSSEGSIGTSRFIVGRDGRRYAFADHNCFACGGDNPIGMHLHIE
                     LGEGTATATWIAGTDYVGWEDKVHGGLIATLLDEVMAWAPSSHDSWAVTAELSVRYRS
                     PASPGEELAARAWVTERRRRIYHVRGEVRGADGRMVAEAEGRYLGASPSTKEALKRRY
                     SLPPPRRAPDKTPATEATAR"
     gene            6993..>8457
                     /locus_tag="VHK28_05825"
     CDS             6993..>8457
                     /locus_tag="VHK28_05825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HEX2141782.1"
                     /translation="MEMDRTGNVRAVAIEDEMRSAYIDYAMSVIVARALPDVRDGLKP
                     VHRRILYTMQEMGLRSTSAYRKCAGVVGEALAKYHPHGDVALYDALVRLAQDFSLRYP
                     LIDGQGNFGSIDGDPPAAYRYTEARMAAIADEMLADIDKETVEFTDNFDGRLREPSVL
                     PARLPNLLINGSSGIAVGMATNIPPHHLGEICDAVTTLIDNPETSVDELCEIVTGPDF
                     PTGGTIFRFEDQRNSLTGEKERVDVIRHMYATGRGRIVIRGQVAFEEIRPGRMAVVVT
                     ELPYQVNKTTLIEKMAELVGSKRVTDVSDIRDESDRTGMRIVVEVKRDGSPHTVMQQL
                     FKHTALQTSFSANMLALVDGQPQTLGLKKMLEHYIGYRREIVRRRTEFDLARAQERAH
                     ILEGLKIALDNLDEVIATIRAAADQDAARTALMKRFSLTEVQANAILEMQLRRLAALE
                     RKKIEDEYEQVIRLIAELEDLLANPRKILQAIKDELAE"
BASE COUNT         1463 a         2903 c         2953 g         1138 t
ORIGIN      
        1 atctgcggcc gccacagggt cccgccgttg gccagcgccg cgtaggcgtt gagcagctgg
       61 atgggcgtga cggccaccac gttctgcccg atgcccgcct gcgccagctc accggtgaac
      121 aggttctcgc ggccctgtga gcgcgcccag gcgcgactgg cgatgatgcc ggccgcctcg
      181 ccgggcaatg acacgccggt gcgctccccg aagccgagct cgttcgccca ggcgccgagc
      241 gcgtccaccc ccagggcgat ggccatctgg tagtagtagg tatccgagct gaccgcgaag
      301 gcgtcgatca tgtccagcgg gccgaagccc tggcgattcc agtcgaagag gcactggccg
      361 gccggggcgc cggggatctg gtagcagccg taggtgggcc agcggcgcgc ggccgaggtg
      421 attccctcct ggagcgccgc cagtccggtc accagcttga aggtcgagcc cggcgggaag
      481 atgtcggcga tggcgtggtt gcgcagcggc ttggccgggt cctcgaggta gacgctgtag
      541 tcctgggcac tgatcccggt ggcgaacttg ttgttgtcgt aggcggggag cgagaccatg
      601 gccaggatct cgccgttctg cgggttcatc accacggtga cgccctgctc cacgccgacc
      661 gcccccatcc cccagcgcag ggcgtcggtg gcgatgcgct gcatgcgggc gtcgatggtc
      721 agcgccaggt tcttgcctgg ctccggctcg ctcaccgtct cgagcgtctt gccgagccgg
      781 ccggccgcgt cgcgttccgc cagctcggcc ccgtacacgc cgcgcagctg ctcctcgaac
      841 gtcgcctcga tccccgcgcg accgatgacg tcgtcgcgca ggtagccatc atccgccagc
      901 tcccggaact cgggaaggtc gaccggcccc acgtagccga ggatgtgcga cagcagcgcc
      961 ccgtccacct cgcccttctc gttgaggtac tgccgaatgg ggtccacctc gaccaccacg
     1021 cccggcaggt tttccgactc ctcgttgatg agcagcgccg cctcccggct gatgccgcgc
     1081 tcgacgggga ccatgtcgaa gggcgaaccg gagtaggcat ccagccggcg gcgcaggtct
     1141 cgcgggtcgc tgccggtgat ctgggccacc cgcagcagga tcccggactc gcgccgctcg
     1201 ggcaggtcgg cgggacgcac cttcaccgtc caggacggca cgttcaccgc gaccgggcgg
     1261 ccctcgcggt cgaagaccag cccgcggacc gagcgcagcg gcacctccac gctgcgggtg
     1321 gcgcgcgcct gggccgcgaa ctgctcgccg ttgaccacct gcagctggaa gaggcggccg
     1381 ccaagcgcgg tgaacagcag cgccgcggcc aggccgaagg ccacgaagcg tgcgcgcgag
     1441 cgcggcaggt ccaggcggcc ctcgttcagg tcggccacgc gcacctcctc accacgccgg
     1501 ccgctcctcg ggcaggacgc gggcggcggc gctgcgcgcg gggtacagca gcaggcccgc
     1561 gatggcggcg ttcagggtgg cggccggcag gatgagctgc agtggagccg gcacgcggag
     1621 cggctcgtcg accagcccga ccacgccgag ggtgacggca tcggccagca cgctgccggc
     1681 gaaggccgcc agcaccgggt agagccagac cagccggccg agtacccggc tgccgccggc
     1741 cacggcggcc gccaccagca gcatggagag cggcaggctg ccgagcgggt cggggaccag
     1801 caggttggcg gtcaggccgg cggcaaaggc ccacacgatc ccgggcccga aacccaggag
     1861 cgaggtcacc agcaccaccg ccaccaggac gaggttgggg cgcacgccgg cgatctcgat
     1921 caccggggac aggccggcgt ggacgatgcc gaccacgacg gcgaggagga cgctggtgta
     1981 gctgatctcg gaccccctgc gggcatgtac acaatcgcca ggttggcgcc aagtatacga
     2041 accgccggct ggcgatccct ataggccgta tcggtccttc atgtcccggt accgcgtcca
     2101 ggcgtggccg tccagctccg gccccatgac ccggtcgagc agcggcacca gctcgtcgcg
     2161 caggtaggcg ccgcgcgcca tccactggta gtagtcacgc ccgccatgcg agtagggccc
     2221 gtacagccgg ccgccccagc gctccaccag ccagcggaac atggcctcat gccgaacgtg
     2281 catgcgcagc gtgatctgcg gaactcgtcc atcaccgccg aaatggccct cgccgaccag
     2341 caggccgacg agcatcccga ggtcgaactc cgtgcccgtt cgctccatgc gtgcgcgcct
     2401 gctagttgtt tcaccgtaag gtatcacgtg caacatcggc cccgctgcag cgtcggggca
     2461 cgcccggcag taagccgcgg ataagcccgc gataagccgc aaactggtag aattcagctg
     2521 cccactgaac tcgcagcgga gggctcgtgg tcagggtcac ggcctgtaca aaccagaagg
     2581 gcggcgtcgg caagacgacg accgtggtga acctggcggc cttcctggcg ctttccggaa
     2641 cctcgactct ggtcatcgac ctcgaccccc agggcaacgc gacgagcggc ctgggtgtcg
     2701 accggcgctc gctggaccga tccgtctatg acgcgatcgt ggaccgaacg cccatcgctg
     2761 acctgatcgt gggaacggcg gtggacgggc tcgacctgct gccatcggcc tcggccctat
     2821 cgggggcgga ggtggagctg gtgcaggtgc ctgcccggga gcggcggctg gaggccagtc
     2881 tcgccgggct ggatggaacg tacgaccggg tgctgatcga ctgcccgccg tcgctcggcc
     2941 tgctgacggt gaatgcgctg acggcggcgg acggggtgct gatccccatc cagaccgaat
     3001 actacgcact ggaggggctg agccagctgg tgaacaccat ccgccgggta cgcgaggcat
     3061 tgaacccgcg gctcgagatc gagggagtgc tgctgaccat gtacgacgcg cggacaaacc
     3121 tctcggcgca ggtcgccgcg gaggtccgcc gccacatgaa cgggaccgtg ttcgacacgg
     3181 tggtgccgcg cagcgtccgg ctttccgagg cgcccagcca cggtctcccg atcgcgctgt
     3241 acgatccggc ctcccgtggc gcgcatgcct acgggcagct ggcggccgag gtcgatgcgc
     3301 gtggctaggc cgttcgggct gggacgcggc ctggacgccc tcatcccgcg ctcagcggaa
     3361 tcgggatccg gatcgctcca gatcccggtc gagcgggtgc accgcaatcc acatcagcct
     3421 cgaaccgcat tcggcgacga gggcctggcc gagctggccg cctccatcgc ggcgcacggt
     3481 gtgctgcagc cgatcgtggt gcgcggtgcg gcagacggcg gctacgagct gatcgccggc
     3541 gagcgccggc tgcgggcggc tcgaatggcc ggcctgaccg agatcccggc catggtgcgc
     3601 gactcccagc cgagcgagct cctcgagctg gcgctggtgg agaacgtgca gcgcgaggac
     3661 ctgaacccga tcgagcaggc aaccgcctac cgcgagctga tcgatcgctt cggcctgtca
     3721 cacgaggcgg tcgcccgcca ggtcggcaag agtcgggtgg cggtcagcaa tgcgctgcgc
     3781 ctcctcgacc tggcgcccga gacgcgtcag gcgatcatcg acggccgcat caccgagggc
     3841 catggccgtg ccctcgcggc gctcaccatt cccgagctgc agcgcgccgt cctgcaggtc
     3901 gtcctggagc gcggcctgtc cgtgcgccag accgaggagc tggtgcggcg caagcgtgag
     3961 accgcacggc cgccgacacg gcaggcgctc agcgaggacc tggccgacgt cgaggcccag
     4021 ctgcgcggca tcctggccac caaggtgggc atcgtccgca cgcggcgcgg tgggcgggtg
     4081 gtcatcgact tttacagcga cgaggagctg gatcgcatct acgccatcat cagccgcggg
     4141 gccggatccc catccgccgc gcacgaggag cgacgggagg accgcgcatg accgttgaac
     4201 cggcgcgcag gacgcgccgc aaggcggacg ggtacaccgc cgccgacatc caggtgctgg
     4261 agggcctgca ggccgtccgc aagcgcccgg gcatgtacat cgggtccacc gatacccgtg
     4321 gcctgcacca gctggtgtgg gaggtggtgg acaacagcat cgacgaggcc atggccaacg
     4381 tggccacccg catcgacgtg acgatccatg ccgacaacag catggaggtg gtggacgacg
     4441 gtcgcggcat cccggtcggc aagcactcca gcggcaagga cgccctcgag gtggtgcaca
     4501 ccgtgctcca cgccggcggc aagttcggcg gcggcgggta caaggtgtcc ggcggcctgc
     4561 acggcgtggg cgtcagcgtg gtgaacgccc tctcaactca catgcgggtc gaggtgctgc
     4621 gcgacggcaa gcgatgggtg caggagtacg cggacggtgg caagccacgc ggcccggtga
     4681 agcagacggg cgggaaggcg gaggccaacc gcggcctgga tcctgactgg aagcgccagc
     4741 acggaacccg cacctttttc acgccggacg cctcgatctt cgagaccctg gactacagct
     4801 gggagacgat cgtcacccgc cttcgagagt ccgcgtacct gaacaagggc gtctggatcc
     4861 gcctggtcga cgagcggacc gatcgggaga agaacttcta cttcgagggc ggggtgacga
     4921 gcttcgtgcg ccacctcaac cggcggcgcg atgtcctcaa cccgaagccc atccacgtcg
     4981 agcgggtggt ggacaccacc agcgtggagg tcgcgctgca gtacaacgac ggattcgcgg
     5041 aaacggtatt ggccttcgcc aacaacatcc ataccgtgga cggcggcagc cacgtggccg
     5101 gcttccgcgc cgcgctgacc gggtcgttga acgactgggc gcgcaagtcg tcgaccctgt
     5161 ctgaaaagga ggccaacctc tcgggcgagg acgttcgcga gggactgacc gcggtcatca
     5221 gcgtcaagct caccgacccg cagttcgagg gccagaccaa ggccaagctt ggcaacgcgg
     5281 acgtgaaggg catcgtccag cgcgtggtga ccgaggggat catccagcac ctggaggaga
     5341 acccggcgga cggacggcgc atcatcgaga agagcctgac cgcggcgcgg gcgcgcgagg
     5401 cggcccgtaa ggcccgcgac ctggtgatcc gcaagggagc gctggacggc atggcgctgc
     5461 ccggcaagct cgcggactgc caggagcgcg acccggcgcg gagcgagctg tacatcgtgg
     5521 agggtgactc ggccggcggg tcggcaaagc agggccgcga ccggcgcttc caggccgtgc
     5581 tgccgctgcg cggcaagctc ctcaacgtcg agaaggcacg cctggaccgc gtgctgtcga
     5641 gcgagaacat tcggccactg atcatcgcgc tgggcgcggg catcggcgac cagttcgaca
     5701 tcgccaagtt gcgctaccac cgcatctgcc tcctatcgga cgccgatgtc gacggagccc
     5761 acatcagcac cctgctgctc accttcttct tccggcacat gccggaggtg atcgacaatg
     5821 gctacctgta cctctgccag ccgcccctgt accgggtgag caccggcaag gtgacccgct
     5881 acgccagcac cgagaaagag cgtgaccagg ccatgaagga gctgtcacgg gagggacgca
     5941 cgaagaacat caccgtacag cgcttcaagg gtctcggcga gatgaacgcc gaccagctgt
     6001 gggagaccac catgaatccc gagacgcgga cgcttctgca ggtgtctgta ggcgacgcgg
     6061 ccgaggccga tgagatattc agcacgctca tgggtgagcg cgtcgagcct cgtcgggact
     6121 tcatccgggg cgaggcccgc aaggtccgca acctggactt ctgacctaag ccgggggcac
     6181 gagagagaac cgatgccaag tcgacacgta gccgacatcc gccaggtccc ggccaagacg
     6241 ggacggaacg tagaggagtg gtgcgagctg ctcgaggcaa acggcgtggg agatcgccgc
     6301 gacgcagtca acttcctgcg cgacgggtac gcgatcagcc acgccttcgc gctggccatc
     6361 gccgaccact gcctgcggcg cagccgggac gacgatttcg aggacccatg ggccggagac
     6421 gacgaggagc ctgcctggtg agctcggaag gctccatcgg gaccagccgc ttcatcgtgg
     6481 ggcgtgacgg ccgccggtac gcattcgcgg accacaactg ctttgcctgc ggcggcgaca
     6541 acccgatcgg catgcacctg cacatcgagc tcggcgaggg aaccgcgacc gccacctgga
     6601 tcgcagggac ggactacgtc ggctgggagg acaaggtgca cggcggcctg atcgccaccc
     6661 tgctcgacga ggtgatggcc tgggcgccgt ccagccacga ctcgtgggcg gtcaccgccg
     6721 agctgagcgt gcgctaccga tccccggcaa gccccggaga ggagctggcg gcgcgggcct
     6781 gggtcaccga gcggcgccgg cggatctacc acgtgcgagg cgaggtgcgt ggcgcggacg
     6841 ggcggatggt ggccgaggcc gagggtcgct accttggcgc cagcccctcc accaaggagg
     6901 cgctcaagcg ccgctactcc ctgccacccc cgcgccgggc acccgacaag acacctgcga
     6961 cagaagcgac agcgaggtag cagacagaac cgatggagat ggacaggacc ggcaacgtcc
     7021 gggcggtggc gatcgaggac gagatgcgtt cggcgtacat cgactacgcc atgagcgtca
     7081 tcgtggcgcg tgcgctgccg gacgtccgcg acgggctgaa gccggtccac cgccgcatcc
     7141 tgtacaccat gcaggagatg gggctgcgct ccacctccgc ctatcgcaag tgcgccggcg
     7201 tggtgggcga ggcgctggcc aagtaccacc cccatggtga cgtcgcgctc tacgacgccc
     7261 tggtgcgcct ggcgcaggac ttcagcctgc gctacccgct catcgacggg cagggcaact
     7321 tcggctccat cgacggggac ccgcccgcgg cgtatcggta caccgaggcg cgcatggcgg
     7381 ccatcgccga cgagatgctg gccgacatcg acaaggagac cgtcgagttc accgacaact
     7441 tcgacgggcg cttgcgcgag ccatcggtcc tgccagcaag gctccccaac ctgctgatca
     7501 acgggtcatc cgggatcgcc gtgggcatgg cgaccaacat cccgccacac cacctgggcg
     7561 agatctgcga cgcggtcacc accctgatcg acaacccgga gaccagcgtc gacgagctat
     7621 gcgagatcgt gaccgggcca gacttcccga ccggggggac gatcttccgc ttcgaagacc
     7681 agcgcaacag cctgaccggc gagaaggagc gggtggacgt catccgccac atgtacgcca
     7741 ccgggcgcgg ccggatcgtc atccgcggcc aggtcgcctt cgaggagatc cgccccgggc
     7801 gcatggcggt agtggtcacc gagctcccct accaggtcaa caagacgacg ctgatcgaga
     7861 agatggcgga gctggtcggc tcgaagcgcg tcaccgacgt tagcgacatt cgcgacgaaa
     7921 gcgacaggac cggcatgcgg atcgtggtgg aggtcaagcg cgacggctcg ccgcataccg
     7981 tgatgcagca gctcttcaag cacaccgcgc tgcagaccag cttcagtgcc aacatgctgg
     8041 ccctcgtcga cgggcagccg cagacgctcg gcctcaagaa gatgctcgag cactacatcg
     8101 ggtatcggcg cgagatcgtg cgccgccgca ccgagttcga cctggcgcgc gcccaggagc
     8161 gggcgcacat cctcgagggg ctgaagatcg cgctcgacaa cctggacgag gtgatcgcca
     8221 ccatccgagc ggccgccgac caggacgccg cacgaaccgc cctcatgaag cgcttctcgc
     8281 tgaccgaggt gcaggccaac gccatcctgg agatgcagct ccgccgcctg gccgcgctcg
     8341 agcgcaagaa gatcgaggac gagtacgagc aggtcatccg cctgatcgcg gagctggagg
     8401 acctgctggc caatccgcgc aagatcctgc aggccatcaa ggatgagctc gccgagc
//
LOCUS       DATACA010000283         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_36019, whole genome shotgun sequence.
ACCESSION   DATACA010000283 DATACA010000000
VERSION     DATACA010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36019"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VHK28_05830"
     CDS             complement(<1..777)
                     /locus_tag="VHK28_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2141783.1"
                     /translation="MASGLPARRRRAAVPPFERSVLPNGLRVVTQEMPLARSVAVGLF
                     VGVGSRHEDARHAGLSHFLEHLVFKGTQAYPDPGGLSEAVEGCGGSVNASTDRELTVY
                     SAKVPALAGDRGLDVVSQLVLRPALRRADLAAEKPVIVDEIRMYQDSPGDHIFTIFDE
                     LLFGAHPLGREIAGTPGSVRRATREAVVAHWRRWYRPEHLVLAVAGATAHDDVVATAA
                     SWFEDGAAWLSEVPAAEAPSVAVLPPEPAPPGDLRLVHRRL"
     gene            complement(779..1249)
                     /locus_tag="VHK28_05835"
     CDS             complement(779..1249)
                     /locus_tag="VHK28_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141784.1"
                     /translation="MSDARAKLYINTGVVTNRSDIYNILRPLLKQTVTYTYFRGNQVT
                     STGTGWVERIVVDHPEIASYFTPLSICLNLDSFDYLQFDTTQEQLLVYTLVIGSERVV
                     IEFAAGGSRLPEGEEGEATGRPLPRTAEELARDLRFIQMELMMSSDAPDEDEDA"
     gene            1307..2155
                     /locus_tag="VHK28_05840"
     CDS             1307..2155
                     /locus_tag="VHK28_05840"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX2141785.1"
                     /translation="MRLLAADVGNTNLSIGLFDDGRLAGSWRASTQASATPDELAALC
                     AQLLALGGASLGDVNAAAIASVVPPLTDALAEALGERADVRPLVVDATTLEGVLPIEI
                     DRPSEAGADRLCNALAARNEVGGPAIVIDLGTSTNFDVLSADGAYIGGAIAPGLGLSL
                     EALVGRASKLPRIELRRPARAIGANTVHAMQSGTVLGYIGLVSGLLTALRGELVERSP
                     AGARVTVIATGGYTHQPWLADVPGIDRVEPDLTLRGIRDAHQAITAGAGRQTGQVAAG
                     GPGAAS"
     gene            2152..3420
                     /gene="coaBC"
                     /locus_tag="VHK28_05845"
     CDS             2152..3420
                     /gene="coaBC"
                     /locus_tag="VHK28_05845"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX2141786.1"
                     /translation="MSEGRGPLAGRRVVVGVTGSIAAYKAVSLVRLLADRGAVVDVAM
                     TPSATQFVTPLTFESLTHRPVVHDVMALDADQQIAHVELAEGADAIVVAPATANMIAE
                     LAAGLVQNAVTAVACASRAPVVVAPAMDAGMWTHPATQRNVETLRGFGYYLVEPEVGA
                     LASGLTGVGRLAEPPRIVEAVERLFARAGELEGLHVVVSAGGTREPLDPVRFIGNRSS
                     GKMGVAIAEAARDRGARVTLVAGAMTASPPRGVQVVDATTAADMREQILRMAPSADIL
                     VMAAAVADFAPKRPSARKIKREGPGLQVELVPNPDIVAEVGEMPEDVRPFLIGFAAES
                     DDLEANATGKLRDKGLDLIVGNVVGGPFDAIGSDENKVTVFGVDGQLTDWPMLPKRQV
                     AERLWDLIVDRYRKGRPAPEARRSSRTRRS"
     gene            3423..4277
                     /gene="panC"
                     /locus_tag="VHK28_05850"
     CDS             3423..4277
                     /gene="panC"
                     /locus_tag="VHK28_05850"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX2141787.1"
                     /translation="MQVVTSAAELRAAILERRMAGDSVGFVPTMGMLHDGSLSLVRRA
                     KAENGCAVVSCFTNPHEFSSLDERDHAARDVPRDLALLEREATDVAFLPEVATLYPPD
                     DLTRVTVEGLTRQLEGASRPGHLDGLTTVMLKLVNLVGPARFYLGQKHAQQVVIIRRM
                     MRDLFMNVDLVVCPTVRATDGLALSSANARLSIEERVAATCLYQAVSAAKRAYDRGER
                     SAEVLRARMTDALAAEPLARTDYVSVADANTLAELQRVSGPALAMVAAWIGRTRLVDN
                     TPLAWDVA"
     gene            complement(4397..>4648)
                     /locus_tag="VHK28_05855"
     CDS             complement(4397..>4648)
                     /locus_tag="VHK28_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEX2141788.1"
                     /translation="WSEATDGADLRGRLAELPSFGDMKVRSMIAVLAKRFGLKLPGLG
                     EVVPQHPTLGDVDSAEALEAYQAAKRAHKAELREAGKRA"
BASE COUNT          646 a         1678 c         1659 g          665 t
ORIGIN      
        1 caggcgccgg tggaccaggc gcaggtcccc tggcggggcc ggctccggcg gcagcacggc
       61 gaccgatggc gcctcggcgg ccggcacctc cgacagccaa gcggctccgt cctcgaacca
      121 ggacgcggcg gtggcgacca catcatcatg ggccgtggcc ccggccacgg ccagcaccag
      181 gtgctctggt cgataccagc gccgccagtg ggcgaccacc gcctcgcgcg tcgcacgccg
      241 cacggagccc ggcgtgcccg cgatctcacg gcccaatggg tgggcgccga acagcagctc
      301 gtcgaagatg gtgaagatgt ggtcgccggg cgagtcctgg tacatccgga tctcgtccac
      361 gatgaccggc ttctccgcgg ccaggtcggc tcgccgcagc gccggtcgca gcacgagctg
      421 cgagacgacg tccagcccgc ggtcgccggc cagcgccggc accttggcgc tgtacacggt
      481 cagctcgcgg tccgtcgagg cgttcacgct gccgccgcaa ccctccactg cctcggacag
      541 cccgccggga tcgggatagg cctgcgtgcc cttgaagacg aggtgctcca ggaagtgcga
      601 caaaccggcg tgccgcgcgt cctcgtggcg gctgccgacc cccacgaaca gcccaactgc
      661 caccgagcgg gccagcggca tctcctgggt gacgacccgc aacccgttgg gcagcaccga
      721 acgctcgaag ggcggcaccg cggcacggcg ccgtcgcgcg gggaggccgg atgccacgct
      781 aggcgtcctc gtcctcgtct ggtgcgtcgg acgacatcat cagctccatc tggatgaagc
      841 gcaggtcgcg tgccagctcc tcggcggtgc gcggcagcgg gcggccggtg gcctccccct
      901 cctcgccctc cggcagccga gatccgccgg cggcgaactc gattaccact cgctcggaac
      961 cgatgaccag cgtgtatacc agcagctgct cctgggtggt atcgaactgg aggtagtcga
     1021 aggagtccag gttgaggcag atggacagcg gcgtgaagta gctggcaatc tcagggtggt
     1081 cgaccacgat gcgctccacc cagccggtgc ccgtgctcgt cacctggttg ccgcggaagt
     1141 aggtgtaggt gacggtctgc ttcaggagcg ggcgcaggat gttgtagatg tcgctccggt
     1201 tggtgacgac cccggtgttg atgtagagct tggcgcgcgc gtctgacatc gggtggcgag
     1261 tctagcagcg gtgggatggc gccacacggc tgctacgatt gccgccgtga ggctgctcgc
     1321 cgcggatgtc ggcaacacca acctgtccat cggcctgttc gacgatggac gcctcgccgg
     1381 ctcctggcgt gccagcaccc aggccagcgc cacgccggac gagctggctg cgctgtgcgc
     1441 ccagctgctg gcgctcgggg gggccagcct cggcgacgtg aatgccgctg ccatcgcatc
     1501 cgtggtaccc ccgttgaccg atgccctggc cgaggcgctc ggcgagcgcg cggacgtgcg
     1561 cccgctggtc gtggatgcga ccacgctaga gggcgttctg cccatcgaga tcgatcgccc
     1621 gtccgaggcc ggcgccgacc ggctgtgcaa cgcgctcgcc gcccgcaacg aggttggtgg
     1681 gccggccatc gtcatcgacc tcgggacgag caccaacttc gacgtgttga gcgccgatgg
     1741 cgcgtacatc ggcggcgcca tcgcgcccgg cctcgggctc tcgctcgagg cgctggtggg
     1801 gcgggccagc aagctgccgc gcatcgagct gcgacgaccg gcgcgggcca ttggcgccaa
     1861 cacggtgcac gccatgcaga gcggcaccgt gctggggtac atcggcctgg tgagcgggct
     1921 gctgacggcc ctccgcgggg agctggtcga gcgctcgccg gctggggcgc gagtcaccgt
     1981 catcgccacc ggcggctaca cccaccagcc ctggctggcc gacgtgcccg gcatcgaccg
     2041 cgtggagccg gacctcaccc tgcgcggcat tcgcgacgcc caccaggcca tcacggccgg
     2101 tgccgggcgg cagaccgggc aggtcgctgc tggcggaccc ggagcggcgt cgtgagcgag
     2161 ggtcgcggcc cgctggccgg gcgccgggtg gtggtcggcg tgacgggcag cattgcggcc
     2221 tacaaggccg tctcgctggt gcgcctgctg gccgatcgcg gcgcggtggt ggacgtggcc
     2281 atgaccccct cggccacgca gttcgtcacg ccactcacct tcgagtccct cacccaccgg
     2341 ccggtggtgc acgacgtgat ggcccttgac gcggaccagc agatcgcgca cgtggagctg
     2401 gcggagggcg cggatgccat cgtggtggcg ccggccaccg ccaacatgat cgccgagctg
     2461 gcggccgggc tggtgcagaa cgcggtgacc gccgtggcct gcgccagccg ggcgccggtg
     2521 gtggtggcgc cggccatgga cgccgggatg tggacacacc cggcgaccca gcgcaacgtc
     2581 gagaccttgc gcgggttcgg ctactacctg gtcgagccgg aggtgggcgc gctggccagc
     2641 ggcctgaccg gcgtgggccg cctggccgag ccgccgcgga tcgtggaggc ggtcgagcgg
     2701 ctcttcgccc gggccggcga gctggagggc ctgcacgtgg tggtcagcgc aggcgggacg
     2761 cgggagcccc tcgacccggt gcgcttcatc ggcaaccgca gcagcgggaa gatgggcgtg
     2821 gccatcgccg aggcggcccg cgaccgcggc gcacgcgtca ccctggtggc cggggccatg
     2881 accgccagcc cgccgcgcgg ggtgcaggtc gtggacgcca ccacggccgc cgacatgcgc
     2941 gagcagatcc tgcgcatggc tccatcggcc gacatcctgg tcatggccgc cgcggtcgcc
     3001 gacttcgcgc ccaagcgccc atcggcccgc aagatcaagc gcgaggggcc gggcctgcag
     3061 gtggagctgg tgcccaaccc ggacatcgtg gccgaggtgg gcgagatgcc cgaggacgta
     3121 cgacccttcc tcatcggttt cgccgccgag agcgacgacc tggaggcgaa cgccaccggc
     3181 aagctgcgcg acaaggggct ggacctcatc gtgggcaacg tggttggcgg cccgtttgac
     3241 gccattggct ccgacgagaa caaggtcacc gtcttcggcg tggacggcca gctgaccgac
     3301 tggccgatgc ttcccaagcg ccaggtggcc gagcggctgt gggacctcat cgtggaccgc
     3361 taccgcaagg ggcgccccgc acccgaggcg cgccgctcgt cccgcacacg ccggtcctga
     3421 gcgtgcaggt cgtcaccagc gccgccgagc tgcgggcggc aatcctcgag cgtcgcatgg
     3481 ccggcgactc ggttggcttc gtgcccacca tgggcatgct gcacgacggg tcgctgtccc
     3541 tggtgcgccg tgccaaggcc gagaacggct gtgccgtggt cagctgcttc accaacccgc
     3601 acgagttctc ctcgctcgac gagcgcgacc atgccgcccg cgacgtcccg cgcgacctgg
     3661 ccctcctcga gcgcgaggcg accgatgtcg ccttcctgcc ggaggtcgcc accctctacc
     3721 cgccggacga cctgacgcgg gtcacggtcg aggggctgac gcgccagctg gagggcgcct
     3781 cccgcccggg ccacctggac gggctgacga ccgtgatgct caagctggtg aacctggtcg
     3841 ggccggcccg cttctacctg ggccagaagc acgcccagca ggtggtcatc atccgccgca
     3901 tgatgcgcga cctgttcatg aacgtggacc tggtcgtctg ccccaccgtg cgcgctacag
     3961 atggcctggc gctgtcgagc gccaacgccc ggctctccat cgaggagcgc gtggcggcca
     4021 cctgcctgta ccaggcggta tcggcggcga agcgcgccta cgaccgcggg gagcgctccg
     4081 ccgaggtgct gcgcgcacgc atgaccgatg cgctcgcggc cgagccgctc gcccgaaccg
     4141 actacgtcag cgtggccgat gcgaacaccc tggccgagct gcagcgcgtc tccgggccgg
     4201 ccctggccat ggtcgcggcc tggatcggcc gcacgcgcct ggtcgacaac acaccgctgg
     4261 cctgggacgt cgcctgagat tctccgttag tactagtgac gtcccgcccc gcatacgtat
     4321 gattcgcgct tgggaggccg gcgagtgccc acgctcggcc tcctcttctg tttgttgacc
     4381 ctcgtgccct cagcacctag gcccgcttgc cggcctctcg cagctccgcc ttgtgagcgc
     4441 gcttggccgc ctggtacgcc tcgagcgcct cggcggagtc cacgtccccc agggtcgggt
     4501 gctgtggcac cacctcgccc aggccgggca gcttcagccc gaatcgcttg gccagcacgg
     4561 cgatcattga gcgcaccttc atgtcgccga acgagggcag ctccgccagc ctgccgcgca
     4621 ggtcggctcc atcagtcgct tccgacca
//
LOCUS       DATACA010000284         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_840239, whole genome shotgun sequence.
ACCESSION   DATACA010000284 DATACA010000000
VERSION     DATACA010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840239"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VHK28_05860"
     CDS             complement(<1..921)
                     /locus_tag="VHK28_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX2141789.1"
                     /translation="MFAVIMAGGVGSRLWPLSRRRSPKQLLALTGDTSLLQQTVARLG
                     ALLKPHDIYVITSQAHVRATQEQLPQLPPDNVLGEPLARSTAVAAGLATVLARRESDE
                     IALVLPADHFISDEPAFAEAMREAVRAAERGYLVTLGIPPKHAATGYGYVKVGERLHP
                     EVPTALVERFVEKPDAERARQFLAEGDYLWNADIFVWRVYAFRQAVERFQPELSEALD
                     RIAALHRTPGWMSDVRDVLEPVPAIQIDAGIAEGAAAEGRVAVVPLDAGWSDIGSWSA
                     LLEALSTQSGHSLVASGRHLDRGSSNVLVQG"
     gene            complement(977..1123)
                     /locus_tag="VHK28_05865"
     CDS             complement(977..1123)
                     /locus_tag="VHK28_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141790.1"
                     /translation="MSRFVLIFAALMLLGAIALSAEGLIGPVAVLTGFVVVMHVRTRP
                     RTDI"
     gene            complement(1190..2035)
                     /gene="proC"
                     /locus_tag="VHK28_05870"
     CDS             complement(1190..2035)
                     /gene="proC"
                     /locus_tag="VHK28_05870"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX2141791.1"
                     /translation="MSPKRDMRVAIVGAGVMAEAMITGLLADRAVAAEQLVASHPRRE
                     RRELLAERHRIAVVPKNAEAVRDAEIVVLAVKPQMLASVMRELGPALRDDQVVLSIVA
                     GANLRTLMTGLLHEAVVRAMPNTPSQIRRGITVWAASGACTARQRDLARAVLRAVGAE
                     KEVADEAFVAMATALSGTGPTYLFAVMEALIDAGVHMGFPRELAHDLVVETLIGSAQF
                     AAQSELHPAQLRNLVTSPGGTSAAAIYELEKGRIRTVFSDAVWAAYRRTLELGERLES
                     DVEHS"
     gene            complement(2032..2652)
                     /locus_tag="VHK28_05875"
     CDS             complement(2032..2652)
                     /locus_tag="VHK28_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141792.1"
                     /translation="MSTSARRSAPIRWGEPGPSGLGRLVARVRALLGEDRPGERQTLG
                     TLPLALQRSAPAAPYLEAAASSDHQRAGAAALVAMEQAVQRAEWWPADIWGHRALWHF
                     EQAGMELAATRAARLIGELRSAAGDPASARRYYAEAISEARDIGAEREQGLAALGLGR
                     AQLELGDVTTARRLAAIAIDLLTRADAPQAELDAARELRGQEKEVG"
     gene            complement(2668..>2928)
                     /locus_tag="VHK28_05880"
     CDS             complement(2668..>2928)
                     /locus_tag="VHK28_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEX2141793.1"
                     /translation="PLAVNVETFGTGTVPERTISEVVMRTFDLRPGAIIGALGLRRPL
                     YRATAAYGHFGRSDLDLPWERTDRAAELRDAAGLPGEAALPA"
BASE COUNT          406 a         1076 c         1059 g          387 t
ORIGIN      
        1 gccctggacg aggacgttgc tcgacccgcg gtcgaggtga cgccccgagg ccaccaggct
       61 gtgcccgctt tgcgtggaca gcgcctccag cagcgccgac caggagccga tgtcgctcca
      121 acccgcgtcg agcggcacga ccgcgacgcg gccctccgcc gcggcgccct cggcgatgcc
      181 ggcgtcgatc tggatggccg gcaccggctc caggacgtcg cgcacgtcgc tcatccagcc
      241 cggagtgcgg tgcagggccg cgatgcggtc cagcgcctcg ctcagctcgg gctggaagcg
      301 ctcgactgcc tgccggaacg cgtagacccg ccatacgaag atgtccgcgt tccacaggta
      361 gtcgccctct gccaggaact ggcgcgcccg ctccgcatcc ggcttctcca cgaagcgctc
      421 caccagggcg gtcggcacct cgggatgcag ccgctccccg accttcacgt aaccgtatcc
      481 ggtggcggcg tgcttgggcg ggatgccgag ggtgaccagg tagccccgct ccgcggcgcg
      541 cacggcctcg cgcatcgcct cggcgaaggc cggctcatcg gagatgaagt ggtcggccgg
      601 caggaccagg gcaatctcgt ccgactcgcg acgcgccagg acggtggcca gcccggcggc
      661 caccgcggtc gagcgggcca gcggttcgcc caggacattg tccggcggca gctgcggcag
      721 ctgctcctgg gtggcccgca cgtgcgcctg ggaggtgatg acgtagatgt cgtgcggctt
      781 gagcagcgcg ccgaggcgcg ccacggtctg ctgcaggagg ctggtatccc cggtcagggc
      841 caacagctgc ttcggcgagc ggcggcgcga cagcggccac aggcggctgc ccacgccacc
      901 ggccatgatg acggcgaaca tctggggaga cgctaccacc ccaaggcggc gccggcgtgg
      961 cccggcgcgc agcgggtcag atgtcggttc gcggccgggt gcggacgtgc atgacgacca
     1021 cgaagccggt cagcacggcc acaggaccaa tgagcccctc cgcgctgagg gcgatggcac
     1081 ctagcagcat gagggcggcg aagatcagga cgaagcggga catgggaagt gctcggtagc
     1141 tcgggaggcg atggtagcag gtgagccgag cgaacccacg tgggggcgcc taggagtgct
     1201 cgacgtccga ctccaggcgc tcaccgagct ccagcgtgcg gcggtaggcg gcccacaccg
     1261 cgtccgagaa gacagtgcgg atgcgcccct tctccagctc gtagatggcc gcggcgctgg
     1321 tgccccccgg cgatgtgacc aggttccgca gctgcgccgg gtgcagctcg gactgcgccg
     1381 cgaactgcgc cgagccaatc agcgtctcga ccaccaggtc gtgcgccagc tcgcgcggga
     1441 agcccatgtg cacgccggcg tcgatgaggg cctccatgac cgcgaacagg tacgtcggcc
     1501 ccgtcccgga gagggcggtg gccatggcca cgaaggcctc gtccgccacc tccttctcgg
     1561 cccccaccgc gcgcaggact gccctggcca ggtcccgctg gcgggcggtg caggcgcccg
     1621 atgccgccca gaccgtgatc ccgcgccgaa tctgggatgg agtgttgggc atggcgcgca
     1681 ccactgcctc gtgcagcagg ccggtcatca gcgtgcgcag gttggcgccg gccacgatgg
     1741 acagcaccac ctggtcgtct cgcagggccg ggcccagctc gcgcatgacc gatgcgagca
     1801 tctgcggctt gacggccagc accacgatct ccgcgtcgcg caccgcctcg gcattcttcg
     1861 gaacgactgc gatccggtgc cgctcggcga ggagctcgcg gcgttcgcgg cgcggatgtg
     1921 aggcgaccag ctgctcggcc gccacggccc tgtcggccag caagccggtg atcatggcct
     1981 cggccatcac ccctgccccg acgatggcca cccgcatgtc ccgcttcggg ctcatccgac
     2041 ctccttctcc tggccacgca gctcgcgcgc ggcgtcgagc tccgcctgtg gcgcatccgc
     2101 ccgggtcagc aggtcgatgg cgatggcggc caggcgccgg gcagtcgtga cgtcgccgag
     2161 ctccagctgc gctctcccca gcccaagcgc cgccagcccc tgctcccgct cggcgccgat
     2221 gtcgcgggcc tcgctgatgg cctcagcgta gtatcggcga gcgctggcgg gatcgccggc
     2281 agccgaccgc agctccccga tcaggcgggc agcgcgggtg gcggccagct ccatgccggc
     2341 ctgctcgaag tgccacagcg cgcggtgccc ccagatgtcg gctggccacc actcggccct
     2401 ctgtaccgcc tgttccatgg cgaccagggc cgccgcgccg gcgcgctggt gatcgctgct
     2461 ggccgcggcc tccaggtacg gggccgccgg tgccgagcgc tggagcgcca gcggcagagt
     2521 gcccagcgtc tgccgttcgc cgggccgatc ctcgcccagg agcgcgcgga cgcgtgcgac
     2581 caggcgtccc agtccggatg ggcccggctc gccccagcgg atgggcgcgg aacggcgtgc
     2641 cgaggtgctc atggggcccg gccgacctca ggccgggagc gcggcctctc cgggcaggcc
     2701 ggcggcgtcc cgcagctcag ctgcgcggtc cgttcgctcc cacggcaggt ccaggtcgct
     2761 cctgccgaag tgaccgtagg cggcggtcgc ccggtagagc gggcggcgca ggcccagggc
     2821 accgatgatg gcgccgggac ggaggtcgaa ggtgcgcatc accacctcgg agatggtccg
     2881 ctcgggaacg gtgcccgtcc cgaacgtctc cacgttgact gccagcgg
//
LOCUS       DATACA010000285         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_438202, whole genome shotgun sequence.
ACCESSION   DATACA010000285 DATACA010000000
VERSION     DATACA010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438202"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1015
                     /locus_tag="VHK28_05885"
     CDS             26..1015
                     /locus_tag="VHK28_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012685284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141794.1"
                     /translation="MLDVRGLQTSFHTRDGIVRAVTGLDFHVNRGEVMGLVGESGCGK
                     SVTSLSILRLIAPPGRIEAGEIMFDGVDLLKLREREMRQLRGERISMVFQQPTSSLNP
                     VYDVGMQVGEVLELHRSMKRKAARDRAMELLRMVGIPDPRRRLDAFPHELSGGMAQRV
                     MIAMALACEPELLIADEPTTALDVTIQAQILDIMRSLQKETGTAIVLITHDLGVVAEM
                     CDRVAVMYAGEIVEQSDVATLFADPRHPYTRGLIGSVPRLGEDREELSVIPGSVPNLV
                     DLPEACRFHPRCTARVELEVPYTDEIHPELRPVAEGHDVRCWIYHERDGSLRT"
     gene            1021..>1532
                     /locus_tag="VHK28_05890"
     CDS             1021..>1532
                     /locus_tag="VHK28_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2141795.1"
                     /translation="MPATTTAPQPAADPRPLVEVERLRQYFPIHGGILQRKLGEVKAV
                     DDVTFTIRRGETLGLVGESGCGKTTVGRTLLRLIEPTEGRVVFDGVDLTALSRRELKA
                     MRRRMQIIFQDPFASLDPRTPIGASIGEGLRVHEIGTVAERRDRVAAMMEKVGLQPYH
                     ARRYPHEFSGG"
BASE COUNT          241 a          516 c          548 g          227 t
ORIGIN      
        1 cggccccgcc gagcctggac agcgcctgct cgacgtccgc ggcctgcaga ccagcttcca
       61 tacccgcgat ggaatcgtgc gggcggtgac cggcctggac ttccacgtga accgcggcga
      121 ggtgatgggg ctggtcggcg agtcaggctg cggcaagagc gtcaccagcc tctcgatcct
      181 gcgcctgatc gcgccgcccg ggcgcatcga ggccggcgag atcatgttcg acggcgtgga
      241 cctgctcaag ctgcgtgagc gcgagatgcg ccagctgcgc ggcgagcgaa tcagcatggt
      301 cttccagcag ccgaccagca gcctgaaccc ggtctacgac gttggcatgc aggtgggcga
      361 ggtgctggag ctccatcggt ccatgaagcg caaggctgcc cgggatcgcg ccatggagct
      421 gctgcgcatg gtgggcatcc cggacccgcg gcggcgactg gatgccttcc cgcacgagct
      481 gtcgggcggc atggcccagc gggtcatgat cgccatggcg ctggcctgcg agccggagct
      541 gctgatcgca gacgagccga ccaccgcgct ggacgtcacc atccaggccc agatcctgga
      601 catcatgcgt tccctccaaa aggagacagg cacggcgatc gtgctcatca cccacgacct
      661 gggggtggtg gccgagatgt gcgatcgggt ggcggtgatg tacgccggcg agatcgtgga
      721 gcagagcgac gtggctaccc tgttcgccga tcccaggcac ccctacaccc gcgggctgat
      781 cggatcggtg ccgcggctgg gcgaggatcg cgaggagctg tcggtcatcc cggggagcgt
      841 gccgaacctg gtggatctgc ccgaggcctg ccgcttccac ccgcgctgca cggcacgcgt
      901 ggagctcgag gtgccctaca ccgatgagat ccatccggag ctgcgcccgg tcgccgaggg
      961 ccacgacgtg cggtgctgga tctaccacga gcgggacggg agcctccgga cgtgaacgcc
     1021 atgcccgcca ccactaccgc gccgcagccc gccgcggatc cccggccgtt ggtggaggtc
     1081 gagcgcctgc gacagtactt tcccatccac ggcggcatcc tgcagcgcaa gctgggcgag
     1141 gtgaaggccg tcgacgatgt gaccttcacc atccggcgcg gtgagacgct cggactggtc
     1201 ggggagtctg gctgcggcaa gacgaccgtg ggacgcacgc tgctgcgcct gatcgagccg
     1261 accgagggca gggtcgtctt cgacggcgtg gatctcacag cgttgtcccg ccgcgagctc
     1321 aaggccatgc gccgccggat gcagatcatc ttccaggacc cgttcgcctc gctcgacccg
     1381 cggacgccca tcggcgccag catcggggag gggctgcgcg tccacgagat cgggacggtg
     1441 gctgagcgcc gtgatcgcgt ggcggccatg atggagaagg tcggcctgca gccgtaccac
     1501 gcccggcgct acccgcatga gttcagcggt gg
//
LOCUS       DATACA010000286         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1001008, whole genome shotgun sequence.
ACCESSION   DATACA010000286 DATACA010000000
VERSION     DATACA010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001008"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VHK28_05895"
     CDS             complement(<1..962)
                     /locus_tag="VHK28_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141796.1"
                     /translation="MRIALATVGTTGDVRPFGALAAGLRAAGHAVTAVSWELHGPALQ
                     EAGAEFVPAGPATSTDEIRSTAAQAAAARSPMDQVAVLRDFHLRDAPAHYRRLRQVLP
                     GHDLVVLHGIHSLAEAAATDAGLRWTSAVFDPVLLPTGTRPPAGMPQLGPSNRAGWWL
                     LDRMLRRLDGPLHAALAEAGSPAPRAVTMFRARSPLLHLVACSPAIVDAPSDLPPTTH
                     LTGAWVPVADPPPLDPALDAFLAAAEPPVVVSFGSMAMDDAESILRIITSAAAAAGVR
                     AVVQLPGGDGIRVEGQDVLLLHAPVDHRALFPRAAAVVHHGGAGT"
     gene            complement(964..1875)
                     /locus_tag="VHK28_05900"
     CDS             complement(964..1875)
                     /locus_tag="VHK28_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX2141797.1"
                     /translation="MLAPPAAPDASTIVPLPAAFDLRLTMAPLAHGTGDPTIRIERQA
                     VWRGTRTRRGPATVLLQEGADGLAVSAWGPGAHDALAGAPELAGLHDDPAALIARHPV
                     LGELQRRYPGVRLPRSGAPFEALLPAICEQKITGAEARRVYRSILRRYGEPAPGPGGL
                     WLAPDPSVLADVPYFAFHPLGLERRRAEVIRRAARTSVELADRSPDEMVAALARLAGI
                     GPWTVAEVRRVALGDPDAVSVGDYHVPSMVAYALAGERRADDARMLELLKPYAGQRGR
                     VQRLLEVGAVFPARRGPRLAPRRIEAL"
BASE COUNT          297 a          741 c          752 g          228 t
ORIGIN      
        1 gtgcccgcac caccgtggtg caccaccgcc gcggcccgcg ggaacaacgc gcggtggtcg
       61 accggcgcgt ggaggagcag gacatcctgt ccctctactc gaatgccgtc gcccccgggg
      121 agctgcacga ccgcgcgcac cccggcagcg gcagccgccg aggtgatgat gcgcaggatc
      181 gattcggcat cgtccatggc catcgacccg aaggagacga cgaccggcgg ctcggcggcc
      241 gccaggaagg catcgagcgc cggatccagc ggaggcgggt cggctaccgg cacccaggcc
      301 cccgtcaggt gagtggtcgg tggcaggtcg gacggtgcgt ccacgatggc tggcgagcag
      361 gcgacaaggt gcagcagcgg cgaccgtgcc cggaacatgg tcaccgcgcg cggcgcgggc
      421 gagccagcct cggccagcgc ggcgtgcagc gggccgtcca gccgccggag catccgatcc
      481 agcagccacc acccggcgcg gttcgacggc cccagctgtg gcatgccggc cggtggccgg
      541 gttccggttg ggagcagcac cgggtcgaag accgcgctcg tccagcgcag tccggcgtcc
      601 gtcgcggccg cctcggccag ggagtggatt ccgtgcagga ccaccaggtc gtggccgggc
      661 agcacctggc gcaggcggcg gtagtgcgcg ggcgcgtccc gcaggtggaa gtcacgcagc
      721 acggccacct ggtccatggg cgagcgggcc gcggccgcct gcgccgcagt cgatcgaatc
      781 tcgtccgtgg aggtcgccgg cccagcaggc acgaactcgg cgcccgcctc ctgaagcgcg
      841 ggcccgtgca gctcccagga gacggcagtg accgcatggc cggcggcgcg caggccggcg
      901 gccagcgctc cgaagggccg cacgtccccg gtggtgccga cggtggcgag cgcaatccgc
      961 acgctacaaa gcctcgatgc gacgcggcgc gagccgcgga ccacggcgcg ccgggaacac
     1021 cgctcccacc tcgagcaggc gctgcacgcg tccacgctgc ccggcatacg gtttgagcag
     1081 ctccagcatc cgggcatcgt ccgcccgtcg ctcgccggcc agggcgtagg ccaccatgct
     1141 gggcacgtgg tagtcgccga cgctgacggc atcagggtct ccgagcgcca cccgccgcac
     1201 ctcggccacc gtccacggcc cgatcccagc cagcctggca agggccgcga ccatctcgtc
     1261 cggggagcgg tcggcaagct cgacgctggt tcgtgcggcg cggcggatga cctcggcacg
     1321 acgccgctcc agcccgagcg gatggaaggc aaagtacggg acgtcggcca ggacggacgg
     1381 gtccggggcc agccacagcc cgcccggccc gggagccggc tcgccatagc ggcgcaggat
     1441 ggaccgatac acccgccgtg cctcggcacc ggtgatcttc tgttcgcaga tggccggcag
     1501 cagcgcctcg aacggcgcgc cgctgcgcgg caggcgcacg cccggatagc ggcgctgaag
     1561 ctcgccgagc accgggtggc gagcgatgag cgcggccgga tcatcgtgca ggccggccag
     1621 ctcgggagcg ccagccagcg cgtcgtgcgc tcccgggccc caggcgctga ccgccaggcc
     1681 gtccgcgccc tcctggagca gcacggtggc tggcccgcgt cgagtgcgcg tgccgcgcca
     1741 tactgcctgt cgctcgatac ggatggttgg atctccggtg ccgtgggcca gcggcgccat
     1801 ggtcaggcgc aggtcgaatg ccgccggcag cggcacgatg gtggatgcat ccggtgcagc
     1861 gggcggcgcc agcatcggcc ggggcatcct catcggcgca tggagaggag gaccggccga
     1921 tccgagcgct ggatgccgtc gaagtccgcg cggccgcgga tggcccggta tccctccatc
     1981 accaccaact gaccgcagat gccacccagc gagttgcc
//
LOCUS       DATACA010000287         3901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2767390, whole genome shotgun sequence.
ACCESSION   DATACA010000287 DATACA010000000
VERSION     DATACA010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767390"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VHK28_05905"
     CDS             complement(<1..290)
                     /locus_tag="VHK28_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2141798.1"
                     /translation="MIDRYALPELRELFGERRRLELWLRIELLAVEALHEAGIVPTQD
                     WERIRQAATDVDPDRAREIEKESQHDVIAFLRSVTERLGPEGRWLHYGLTSS"
     gene            complement(649..1152)
                     /gene="purE"
                     /locus_tag="VHK28_05910"
     CDS             complement(649..1152)
                     /gene="purE"
                     /locus_tag="VHK28_05910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX2141799.1"
                     /translation="MVSDTPSVAVICGSRSDLPALRGCFDALDRYAIPWEASVISAHR
                     QPEALGTYVRDAEARGVKVFVGAAGLAAHLPGVLASLTARPVIGVPMDGGALGGADAL
                     YSIVQMPPGVPVAAVAIGSAGARNAGHLAARILALADADLARRVAAFREEQAGGAEVG
                     IDPRAGS"
     gene            1264..2520
                     /locus_tag="VHK28_05915"
     CDS             1264..2520
                     /locus_tag="VHK28_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009778358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2141800.1"
                     /translation="MLVQPLHRSTSVVLAVVALSALLLAAVLVPPVVQAAPRGPQSVR
                     FATFNASLNRNNAGDLVSDLSTQTNAQASAVAEIIQRVRPDVLLINEFDFDPAAVDLF
                     RDNYLEQPHNGAAPIEYPYAFIDESNTGVPSGFDLNNNGAIGGPDDAFGFGFFPGQFG
                     MLVLSRYPIQSDAVRTFQHFLWKEMPGAMLPDDPSTPAPADWYSPEELQVFRLSSKSH
                     WDVPIDVGGTTVHFLVSHPTPPVFDGPEDRNGTRNFDEIRFWADYVTPGAGGYIYDDA
                     GGTGGLKPVEKFVIAGDQNSDPLDGDSIPGSIQQLLENPRINTRVTPDSEGAVDATER
                     QMGINLIHRSDPRFDTADFSDSAPGNLRADYVLPSRNLRILDAGVFWPVDESPLFRLV
                     GDFPFPSSDHRLVWVDVHVPGAERGH"
     gene            complement(2528..3802)
                     /gene="purD"
                     /locus_tag="VHK28_05920"
     CDS             complement(2528..3802)
                     /gene="purD"
                     /locus_tag="VHK28_05920"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX2141801.1"
                     /translation="MEILVLGGGGREHALAWRLARDEAVRRVRIAPGNGGTPATAETV
                     AGLDPLDPVAVARHAGRERYDLVVIGPEGPLASGVADALAQAGIPAFGPSRDAARLES
                     SKAFAKEQMRRAGVPAAESADFVTVDDALGHARRMDPPPVVKADWLAAGKGVVVPDSA
                     EEAEIAIRTLFEHSEPGSRLLLEERLTGREVSVFALVSDQAVVPLGAARDYKRLQDGD
                     IGPNTGGMGAYAPVSWFGTPAVEAVVESVFEPIAWRMSRDGTPYRGVLYACLMVTADG
                     PKVLEFNARFGDPEAQVLLPLLDGQLSAALLGTATGDRAAMEGAIGLRPGAAVGVVLA
                     AEGYPEAPASGRALVGADPAGPQHDGPSLCFHAGTRAGQDGGHQAAGGRVLTMVGVAG
                     DPSTARQVAYDAMANVRLEGGQYRTDIAAELG"
BASE COUNT          563 a         1474 c         1324 g          540 t
ORIGIN      
        1 tcggagctgg tgaggccgta gtgcagccag cggccctccg gtccgagccg ctccgtgacc
       61 gaccgcagga aggcgatcac gtcgtgctgc gactccttct cgatctccct ggcgcggtcg
      121 gggtcgacgt cggtcgccgc ctgtcggatg cgctcccagt cctgggtggg gacgatgccc
      181 gcctcgtgca gcgcctcgac ggccagcagc tcgatccgca gccagagctc cagccggcgc
      241 cgctcgccga acagctcgcg cagctcgggc agggcgtagc gatcgatcac cccggaagga
      301 tagggcgacg gcggcgggag cgcggctgcg gctgtggaac cgtgcgaatg tcccacggtc
      361 ttgtgatggg gccatggcca tccgggcgtt gtgcgccgat gtggcgggca gaggttcacg
      421 ggagggtgcc ccggcgtgtc gttctgccga tgtatcgggc agccgtgagc gtgagcggtg
      481 aagtttttgt cggttcgtgc tgcgaactgc cccatgaatc ggcgttctga cacgccacac
      541 tcggtccggg tgaagaactg cccgatgtag cggcgccagc taggcccgcg acctagaccg
      601 gggggacctc gagggcggcg cggccgccgg gccagggctg gccgggcctc agctccctgc
      661 gcgcgggtcg atccccacct cggccccgcc ggcctgctct tcgcgaaaag cggccactcg
      721 ccgagccagg tcggcatcgg ccagggcgag gatgcgcgcc gccaggtgcc cggcgttgcg
      781 ggcgccggcc gaaccgatcg ccaccgcggc aaccggaacc ccgggaggca tctgcacgat
      841 cgagtacagg gcgtcggcac cacccagggc gccaccatcc atgggtacgc cgatcacagg
      901 ccgtgcggtc aagctggcca gcacgccggg caggtgggcg gccagccccg cagcacccac
      961 gaagaccttg acgccgcgcg cctccgcatc gcgcacgtag gtcccgagcg cctccggctg
     1021 gcggtgcgcg ctgatgaccg acgcctccca ggggatcgcg tagcggtcga gcgcgtcgaa
     1081 gcagccgcgc agtgccggca ggtcggagcg ggagccgcag atgacggcca cggacggggt
     1141 gtcggacacg atgacccgaa ggatagcgag ccagttgccc tttggcgtac gctggacgcg
     1201 acggactctc ccgtgctgcc cccgagcgtt ccgcggcacc gcagacgaca aggaggcatc
     1261 ggcatgctgg tccaacccct ccatcggtcc acctcggtgg tcctcgccgt cgtggcgctg
     1321 agcgccctgc tcctggcggc ggtcctggtc ccgccggtgg tgcaggcagc accgcgaggc
     1381 ccacagtcgg tccgcttcgc gaccttcaac gcctcgctga accgcaacaa cgccggcgac
     1441 ctggtatccg atctctcgac ccagaccaac gcccaggcct cggccgtggc cgagatcatc
     1501 cagcgcgtcc ggcccgacgt gctgctcatc aacgagttcg acttcgaccc ggcggcggtc
     1561 gacctgttcc gcgacaacta cctggagcag ccgcacaacg gcgcggcacc catcgagtac
     1621 ccgtacgcct tcatcgacga gtcgaacacc ggggtcccat ccgggttcga cctcaacaac
     1681 aacggggcca tcggcgggcc tgacgacgca ttcggcttcg gcttcttccc cggccagttc
     1741 ggcatgctgg tgctgtctcg ctacccgatc cagagcgatg cagtgcgcac cttccagcac
     1801 ttcctgtgga aggagatgcc cggcgccatg ctccccgacg acccgagcac cccggctcca
     1861 gcggactggt actcacccga ggagctccag gtcttccgcc tctcgtcgaa gtcgcactgg
     1921 gacgtgccca tcgacgtcgg cggcaccacc gtccacttcc tggtgagcca tccgacgccg
     1981 ccggtcttcg acgggccgga ggaccggaac gggactcgca acttcgacga gatccgcttc
     2041 tgggccgact acgtcacgcc gggcgcgggc ggctacatct atgacgacgc gggcggcacc
     2101 ggcggcctca agcccgtaga gaagttcgtg atcgccggag accagaactc cgacccgctc
     2161 gacggcgact cgatccccgg ctccatccaa cagctgctcg agaacccgcg gatcaacacc
     2221 cgggtcacgc cggacagcga gggcgcagtc gatgccacgg agcggcagat gggcatcaac
     2281 ctcatccatc gcagtgaccc gcgcttcgat accgctgact tcagtgacag cgcgcccggc
     2341 aacctgcgcg ccgactatgt cctgcccagc cgcaacctgc ggatcctgga cgcgggcgtc
     2401 ttctggccgg tggacgagtc accgctcttc cggctcgtcg gagacttccc gttccccagc
     2461 tcggaccacc gcctggtgtg ggtcgacgtg cacgtccccg gcgcggagcg cggccactga
     2521 tcgaccgtca gcccagctcg gccgcgatgt cggtccggta ctgcccgccc tccagccgga
     2581 cgttggccat cgcgtcgtag gcgacctggc gcgcggtgga cgggtcgccg gccacgccga
     2641 ccatggtcag cacccgcccg ccggcggcct ggtgtccgcc atcctggccg gcccgcgtgc
     2701 cggcgtggaa gcagagcgac ggtccgtcgt gctgcggccc ggccgggtct gcgccgacca
     2761 gcgcgcgccc gctggctggt gcctccgggt agccctcggc ggccagcacc acgcccaccg
     2821 cggcgccggg gcgcaggccg atggcgccct ccatggcggc gcggtcgccg gtggccgtgc
     2881 ccagcagggc ggctgacagc tggccgtcca gcagcggcag caggacctga gcctccgggt
     2941 cgccgaagcg ggcgttgaac tccagcacct tcggtccatc cgcagtgacc atcaggcagg
     3001 catatagcac gccgcggtac ggggtgccgt cacgcgacat gcgccaggcg attggctcga
     3061 agacgctctc caccaccgcc tccaccgccg gcgtgccgaa ccaggaaacg ggcgcgtacg
     3121 cccccatgcc cccggtgttg ggaccgatgt cgccgtcctg cagccgcttg tagtcgcgtg
     3181 ccgctcccag cgggaccacc gcctgatcgc tgaccagggc gaagaccgat acctcccgcc
     3241 cggtcagccg ctcctcgagc agcaggcggc tcccgggctc ggagtgctcg aagagggtcc
     3301 ggatggcgat ctccgcctcc tccgcgctat cgggcaccac cacgcccttg ccggccgcca
     3361 gccagtccgc cttgaccacc ggcggcgggt ccatgcgccg cgcgtggccc agcgcgtcgt
     3421 cgaccgtgac gaagtcggcg ctctccgcgg ccgggacgcc ggcgcggcgc atctgctcct
     3481 tggcgaacgc cttcgaggac tccagccgcg ccgcatcccg cgagggaccg aaggccggga
     3541 tgccggcctg cgccagcgca tcggccaccc cggaggccag cgggccctcc ggcccgatga
     3601 ccaccaggtc gtaccgctcc cgcccggcgt gcctggccac ggccaccgga tccagcggat
     3661 ccaggcccgc gaccgtctcg gccgtggccg gcgttccgcc gttgccaggg gcgatccgca
     3721 cccgccgcac cgcctcgtcc cgggccaggc gccaggccag ggcatgctcc cgcccaccgc
     3781 cgcccaggac caggatctcc atcagggcga ggggctcgcc tcgggctccg gcgactcagg
     3841 gtccggttcc gcgccggtca gctcggcaag ggcctccagg atcggctcga gctcgttgag
     3901 g
//
LOCUS       DATACA010000288         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2687553, whole genome shotgun sequence.
ACCESSION   DATACA010000288 DATACA010000000
VERSION     DATACA010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687553"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VHK28_05925"
     CDS             complement(<1..648)
                     /locus_tag="VHK28_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX2141802.1"
                     /translation="MGALSRHRLAIFVALVGAVLVWIIWSARGALPAFFIGAALAFVL
                     DPVVTFLQRRGLPRWAGIVVAYFMVVGLIWALIAYALPPIADQTREFITHLPELGAQL
                     SGVQQSVLDWYASLPLPPDLREMLDESIANANEAFANALREFLGPAVTAVLRTAAFIV
                     GLVIVPIWLFFVLKDRERLPRAVAGALPESWRADAANVMAILARVGGRWVRGQLLL"
     gene            688..1440
                     /locus_tag="VHK28_05930"
     CDS             688..1440
                     /locus_tag="VHK28_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX2141803.1"
                     /translation="MNRTATPPTTERASGSGSSPRQRWQILFSRREPALRIRQADLLR
                     EFERAFRDAGLPLSETNAAKPRPRMKFAANLPVGIELRGDVLEVYFDELVPMDRVMAA
                     AELLPEGVGVEDAREVWHGFPSAASQVRGAEYEVEVRARGDVQLTSDELRGAVVRLLS
                     ARRLVGKRRRGESERRADTGARDLRPFIEDVEVLEVDEGSGHGRLRMALRLDPSGAGR
                     PEDVVNALDTPLEVVRAARTRLLFVDTPPIAR"
     gene            1533..2111
                     /gene="rplU"
                     /locus_tag="VHK28_05935"
     CDS             1533..2111
                     /gene="rplU"
                     /locus_tag="VHK28_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX2141804.1"
                     /translation="MYAVVSSGGKQYRVEAGTTLTMERNGAEAGASLTFDRVLLIGDG
                     DQVTVGTPVVPGASVSATVLGEQLGPKLVIFKFKQKVKYRRRTGHRQKLIRVRIDDIS
                     AGGAKREKAQPAKAEPEADAAEASVQAEPKPKAPKRTAKPKAEPKAEAASEAKPKTTR
                     RTAKPKAADGETAADAPKAEKARARKAPKAEE"
     gene            2116..>2358
                     /gene="rpmA"
                     /locus_tag="VHK28_05940"
     CDS             2116..>2358
                     /gene="rpmA"
                     /locus_tag="VHK28_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX2141805.1"
                     /translation="MAHKKAGSSSKNGRDSAGKRLGVKRGDGQIVLSGTIIVRQRGST
                     FTPGPNVGMGRDYTLFATADGTVRFSHLTRDKKQVSV"
BASE COUNT          416 a          824 c          849 g          269 t
ORIGIN      
        1 cagcagcagc tgtccgcgca cccatcggcc accgacccgc gccaggatgg ccatgacgtt
       61 ggcggcgtcc gcgcgccacg actcgggcag cgccccggcc accgctctcg ggaggcgctc
      121 ccggtccttg agcacgaaga agagccagat cggaacgatg accaggccga cgatgaaggc
      181 cgcggtgcgc agcacggcgg tgaccgccgg gcccaggaac tctcgcagcg cgttggcgaa
      241 cgcctcgttg gcgttggcga tcgactcgtc cagcatctcc cgcaggtcgg gcggcagggg
      301 cagggacgcg taccagtcca ggaccgattg ctgcaccccg ctcagctggg ctcccagctc
      361 cggcaggtgg gtgatgaact cgcgggtctg gtcggcgatg ggcggcagcg cataggcgat
      421 cagggcccag atcagcccga cgaccatgaa gtaggccacc acgatcccag cccagcgcgg
      481 caggccgcgg cgctggagga aggtgaccac cgggtccagt acgaacgcca gcgcggcacc
      541 gatgaagaag gctggcagcg cgccgcgggc cgaccagatg atccagacca gcaccgcgcc
      601 gaccagcgcg acgaagatgg caaggcgatg gcgggagaga gcacccatga gatcagccat
      661 cgcgcgcacg gtaccattca ggtagacatg aaccgcactg ccacgccacc caccaccgaa
      721 cgcgccagcg gcagcggcag ttcgccgcgg cagcgctggc agatcctgtt ctcgcgccgc
      781 gagccggcat tgcgcatccg ccaggccgac ctgctgcgcg agttcgagcg cgccttccgc
      841 gacgctgggc tgccgctctc cgagacgaac gccgccaagc cgcgcccgcg catgaagttt
      901 gccgccaacc tgccggtcgg catcgagctg cgcggcgacg tgctcgaggt ctacttcgac
      961 gagctggtgc ccatggaccg cgtgatggcc gccgccgagc tcctgccgga gggcgtcggg
     1021 gtggaagacg cccgcgaggt gtggcacggc ttcccgtcgg cggcctccca ggtgcgcggc
     1081 gcggagtacg aggtcgaggt gcgggcgcgt ggtgacgttc agctcaccag cgacgagctg
     1141 cgcggcgccg tcgtccggct gctgtccgcc cgccggctgg tgggcaagcg gcgtcgcggt
     1201 gagagcgagc gcagggcgga caccggggcg cgcgacctgc ggccattcat cgaggacgtg
     1261 gaggtcctgg aggtcgacga gggctccggc cacggccgcc tgcgaatggc cctgcgcctg
     1321 gacccgtcgg gtgccggccg gccggaggac gtggtgaacg ccctggacac gccgctggag
     1381 gtcgtcaggg cggctcggac ccgcctcctg ttcgttgaca ctccccccat cgcccggtag
     1441 acttcgagct cccgcgggct gagaacggcc cgcgtcgtcg tgcgcgccgc agcgcgggca
     1501 cgagcgcaac cccagacctt tcggagatgt tcatgtacgc cgtcgtcagc agtggcggga
     1561 agcagtaccg cgtcgaggcc ggcaccacgc tcaccatgga gcggaacggt gcagaggcgg
     1621 gcgcgtcgct caccttcgac cgcgtcctgc tcatcggcga cggtgaccag gtgacggtcg
     1681 gcacgccagt ggtgcccggg gcatcggtca gcgcgaccgt gctcggcgag cagctgggac
     1741 ccaagctcgt catcttcaag ttcaagcaga aggtcaagta ccgccgtcgc accgggcacc
     1801 gccagaagct gatccgggtc cgcatcgacg acatcagcgc cggtggcgcc aagcgcgaga
     1861 aggcccagcc cgccaaggcc gagccggagg cggacgccgc ggaggcatcg gtccaggcgg
     1921 agcccaagcc gaaggccccg aagcggacgg cgaagcccaa ggcagaaccc aaggccgagg
     1981 cggcgtcgga ggccaagccg aaaacgacgc gtcgcaccgc caagccgaag gcagccgacg
     2041 gcgagacggc cgcggacgcc ccgaaggcag agaaggccag ggcccgcaag gcccccaagg
     2101 ccgaggagta acgacatggc acacaagaag gccggcagca gcagcaagaa cggccgcgat
     2161 tcggccggca agcggcttgg cgtcaagcgt ggcgacgggc agatcgtgct gtccgggacc
     2221 atcatcgtcc gccagcgcgg cagcaccttc acgcccggcc ccaacgtggg gatgggccgc
     2281 gactacacgc tgttcgccac cgctgacggc acggtcaggt tcagtcacct gacccgcgac
     2341 aagaagcagg tcagcgtg
//
LOCUS       DATACA010000289         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_518136, whole genome shotgun sequence.
ACCESSION   DATACA010000289 DATACA010000000
VERSION     DATACA010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518136"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1470
                     /locus_tag="VHK28_05945"
     CDS             28..1470
                     /locus_tag="VHK28_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2141806.1"
                     /translation="MTTEVSAPTVPIYVAGEFVEAGCPLNVHNPATGELIATTFQGGP
                     SELERATAAAVDAFERTRRLASYQRRDALAHVAERIARDTDELADLLSRESGKPIRDA
                     RAEVSRASLTFRTAGEEALRITGEWLPLDWNATNRGRSAIVRRYPIGPVAGISPFNFP
                     LNLAAHKVAPALAAGCSIVLKPPSRDPLVMLRVAEYINETDLPAGSVSILPMDRPTGD
                     AMVGDDRFKLLSFTGSPSVGWKMKAEAGKKKVVLELGGNAGAIVDESADLDWAVSRLV
                     FGSFAYAGQVCISVQRIYVVESVWDAFVERFTAAVRQVKVGDPLDQATDLGPMVDRAA
                     VERTHEWVTEAVERGARALTGGQPDGQHYPPTVLVDVPKDARVCGEEVFAPVVNLFGV
                     PDFGAALREINDSRFGLQCGVFTNDLERTLTAHDELEVGGVIVNDIPTWRIDPMPYGG
                     VKDSGLGREGLRYSIEDMTEPRLLAFARPL"
     gene            1473..2270
                     /locus_tag="VHK28_05950"
     CDS             1473..2270
                     /locus_tag="VHK28_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX2141807.1"
                     /translation="MDPELERRLRAAGVDPYDALDPREAFVRLHAVEGQRATLLDRYA
                     LEAASRGVPVNVLPEQLRTELQAEVLRLHVPGWEIVGGSDRSTTDPIRIVDYDPAWPT
                     LFQEWCGRLAEALGDGATRIDHVGSTAVPCLAAKPIIDVQISVPDVNDEATYVSAIER
                     AGVAFRARDPEHRYFRPSGDRPRAVQVHVCQAGSGWERTHLLYRDFLRANPETRDAYA
                     ALKRDLAARYRDDRIAYTEAKTSFILDTMSRAAAWAAQSGWRVGDAA"
     gene            2267..2611
                     /locus_tag="VHK28_05955"
     CDS             2267..2611
                     /locus_tag="VHK28_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2141808.1"
                     /translation="MTDTTRYARVLRAHELRDGELIPVEIDGTPVVLVRHAGEFFAVQ
                     NNCSHRDFPLSAAGFDPRDEVLVCAWHGGCFDIRSGEAVVPPATEPVETFPVRVSAEG
                     WVEIGLTGSLAG"
     gene            2637..3416
                     /locus_tag="VHK28_05960"
     CDS             2637..3416
                     /locus_tag="VHK28_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141809.1"
                     /translation="MSLEALPFVNWIFWAALGSGTLLVVGLTELLGGTTRGYRLFMAG
                     LVVVFAAILLLSELNLAPGTEADRTADLRRSLVWAFCAATVAYLIASVARSPRSGLAI
                     VGGGLGMISLATLAAAGGTLSSMLFAAQLVLVALALGSVNAAMLLGHWYLVTPKLSPD
                     PLRRMMWLLIGVLVLQAVAFGVAVLTISSGPLGGPIGWLTWLRLLVGIALPIVITVLA
                     ILASRAASLQSSTGLLYIGLAFVMAGSIAGASITYLTGVPV"
     gene            3422..4078
                     /locus_tag="VHK28_05965"
     CDS             3422..4078
                     /locus_tag="VHK28_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX2141810.1"
                     /translation="MQITLRTFAQLRDLSVDRLMVDLPEGSRMTDGWAALAERHPAVE
                     PHRPYVLAARNGAYAAWDEVLADGDEVAFLPPVSGGGPCGLTDGVIDVAALERAVADP
                     GHGALVTFIGRARDTADDGRTVLELEYEAYAEMAESVLAEIVAEAELRWEGSRVAVVH
                     RMGRVPIGEAAVAIVTASAHRAEAYEANRQVIEAIKQRLPIWKSERFADGTEWKRPGA
                     "
     gene            4123..>4341
                     /locus_tag="VHK28_05970"
     CDS             4123..>4341
                     /locus_tag="VHK28_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141811.1"
                     /translation="MTPKPGQIRCPTCHRSTPPAAYCTQCGSPIPASARVRPRGMDRD
                     ELEERIRGRRPGEGFRRGSAPDEAFPEEG"
BASE COUNT          593 a         1516 c         1559 g          673 t
ORIGIN      
        1 cgggacgcac agcgtagaat cgctttcatg acgaccgagg tcagcgctcc caccgttccc
       61 atctatgtcg ccggggagtt cgtggaggcc ggctgcccgc tgaacgtcca caacccggcc
      121 accggcgagc tgatcgccac caccttccag ggtggccctt ccgagctgga gcgcgccacc
      181 gcggccgcgg tcgatgcctt cgagcgcacc cgccgcctgg ccagctacca gcgccgcgat
      241 gccctggccc acgtggccga gcggatcgcg cgggacacgg acgagctggc cgacctgctg
      301 tcccgcgagt cgggcaagcc gatccgcgac gcccgggccg aggtctcgcg ggccagcctc
      361 accttccgga ccgccggcga ggaggcgctg cgcatcaccg gggagtggct gccgctggac
      421 tggaacgcga cgaaccgggg ccggagcgcc atcgtgcgcc gctacccgat cgggccggtg
      481 gccggcatca gcccgttcaa cttcccgctg aacctggccg cccacaaggt ggcgccggcc
      541 cttgccgcgg ggtgcagcat cgtcctcaag ccgccatcca gggaccccct ggtcatgctg
      601 cgcgtggcgg agtacatcaa cgagacggac ctcccggcgg ggagcgtgag catcctgccc
      661 atggatcgcc caaccgggga cgccatggtg ggtgacgacc gcttcaagct gctgtccttc
      721 accggctccc ccagcgtcgg ctggaagatg aaggccgagg ccggcaagaa gaaggtcgtg
      781 ctggagctgg gcggcaacgc gggcgccatc gtggacgagt cggcggacct ggactgggcg
      841 gtcagccgcc tggtgttcgg cagcttcgcc tatgccggac aggtctgcat cagcgtgcag
      901 cgcatctacg tggtggagtc ggtgtgggac gccttcgtcg agcgcttcac cgccgccgtg
      961 cgccaggtga aggttggcga tccgctggat caggccaccg acctggggcc tatggtcgac
     1021 cgcgcggcgg tcgagcggac ccacgaatgg gttaccgagg cggtggagcg tggtgctcgg
     1081 gcgctgaccg gcggccagcc ggatgggcag cactaccctc ccaccgtgct ggtcgacgtg
     1141 ccgaaggacg cgcgcgtctg cggcgaggag gtcttcgccc cggtggtcaa cctgttcggc
     1201 gtgcccgatt tcggcgcggc cctgcgcgag atcaacgact cgcgcttcgg gctgcagtgc
     1261 ggagtcttca ccaacgacct ggagcggacg ctgacggcgc acgacgagct ggaggtgggt
     1321 ggcgtcatcg tcaacgacat ccccacctgg cggatagacc ccatgcccta tggcggcgtc
     1381 aaggattccg ggctggggcg cgaggggctg cgctacagca tcgaggacat gaccgagccg
     1441 cggctgctgg ccttcgctcg cccgctgtag gcgtggaccc cgagctggag cggcggctgc
     1501 gtgccgcagg ggtggatcct tacgacgcgc tcgacccgcg cgaggccttc gttcggttgc
     1561 acgcggtcga ggggcaacgc gcgacgctgc tggaccgcta tgcgctcgag gcggcgtcgc
     1621 gcggcgtgcc agtgaacgtc ctgcccgagc agctgcgaac ggagctgcag gcggaggtcc
     1681 tgcgcctcca cgttccgggc tgggagatcg ttggtgggtc cgaccgctcg accacggatc
     1741 cgattcgcat cgtggactac gatccggcct ggccgacgtt gttccaggag tggtgcggcc
     1801 gcctggcgga ggcgctgggc gatggcgcca cgcgtatcga ccacgttgga tcgaccgccg
     1861 tgccttgcct ggccgccaaa ccgatcatcg atgtccagat cagcgttccg gacgtcaacg
     1921 atgaagcgac gtacgtgtcg gccatcgagc gtgccggcgt ggccttccgg gcgcgggatc
     1981 ccgagcatcg ctacttccgg cccagcggcg accggccacg cgccgtccag gtccacgtct
     2041 gccaggccgg atcgggctgg gagcgaaccc atctcctcta ccgcgacttc ctgcgggcaa
     2101 atcctgagac ccgcgatgcc tacgcagccc tgaagcgcga cctcgcggcc cgctaccgtg
     2161 atgacaggat cgcctatacc gaggccaaga ccagcttcat cctggacacc atgtcgcgcg
     2221 ccgcggcctg ggccgcccag tctggctggc gcgtgggaga cgcggcgtga cggacaccac
     2281 ccgctatgcg cgcgtgctgc gcgcccacga gctgcgcgac ggcgagctga tccccgtgga
     2341 gatcgacggc accccggtgg tgctggtccg ccatgcgggc gagttctttg cggtgcagaa
     2401 caactgcagc caccgcgact tccccctgtc cgcggcgggc ttcgacccgc gtgacgaggt
     2461 cctggtgtgc gcctggcacg gcggctgttt cgacatccgc agcggggaag ccgtggtccc
     2521 gcctgccacc gagccggtgg aaaccttccc cgtccgtgtc agcgccgagg gctgggtcga
     2581 gatcggcctc accggttcgc tggccgggtg agacggccgt accgggaccg cgcggcatga
     2641 gcctcgaagc cctcccgttc gtgaactgga tcttctgggc cgcgctgggc tccggaaccc
     2701 tgctggttgt cggcctcacc gagctgctcg gcgggacgac ccgcggctat cggctcttca
     2761 tggcgggcct ggtggtcgtc ttcgccgcca tcctgctgct ctcggagctg aacctggcgc
     2821 ccggcacgga ggcggatcgc accgcggacc tgcgccgcag ccttgtctgg gccttctgcg
     2881 cggccaccgt cgcctacctg atcgcctcgg tggcccggtc gccgcgctcc ggcctggcca
     2941 tcgtcggggg cgggctcggc atgatctccc tggcaacgct ggccgcggcc gggggaacgc
     3001 tcagctccat gctcttcgcc gcgcagctgg tcctggtcgc gctggccctc gggtcggtga
     3061 acgcggccat gctgctgggg cactggtacc tggtcactcc caagctgtcg ccggatccgc
     3121 tgcgtcgcat gatgtggctg ctgatcggcg tgctggtcct gcaggcggtg gccttcgggg
     3181 tggccgtgct gaccatcagc agcgggccgc tgggcgggcc catcgggtgg ctgacctggc
     3241 tgcgcctgct ggtcggcatc gcgctgccga tcgtgatcac ggtgctggcc atcctcgcca
     3301 gccgtgccgc cagcctgcag tcgagcaccg gactgctgta catcggtctg gcattcgtca
     3361 tggccggctc cattgccggg gccagcatca cctacctgac cggcgtgccc gtctgagacc
     3421 gatgcagatc acgctccgca ccttcgcgca gctccgggac ctgtcggtgg accggcttat
     3481 ggtcgacctg ccggaggggt cacgcatgac cgatgggtgg gccgcgctgg cggagcgcca
     3541 ccctgccgtg gagccgcatc gcccgtacgt gctggcggcg cggaacggtg cctacgccgc
     3601 ctgggacgag gtcctggccg acggcgacga ggttgccttc ctgcccccgg tgtccggtgg
     3661 cggaccctgc ggcctgaccg atggcgtcat cgacgtcgcc gcgctcgaac gagcggtcgc
     3721 tgacccgggg cacggcgcgc tggtgacctt catcggtcgc gcgcgcgaca ccgccgacga
     3781 cgggcggacg gtgctggagc tggagtacga ggcctatgcc gagatggctg aatcggtcct
     3841 ggccgagatc gtggccgagg ccgagctgcg ctgggagggg agccgggtcg cggtcgtcca
     3901 ccgcatgggc cgggtgccga tcggtgaggc ggcggtggcc atcgtgaccg cctccgccca
     3961 ccgcgcggag gcctacgagg cgaatcgcca ggtcatcgag gccatcaagc agcggctgcc
     4021 catctggaag agcgagcgct tcgcggatgg caccgagtgg aagcgtcctg gagcctaggc
     4081 gttggcacga cgcggtgaca gcgtgcacaa tgccgcactg acatgacccc caagcccggg
     4141 cagatccgct gtcccacctg tcatcgctcc acgccaccgg ctgcctactg cacgcagtgc
     4201 gggtcgccga tcccggcctc cgcgcgcgtc cggccgcgtg gcatggaccg cgacgagctg
     4261 gaggagcgca tccgtgggcg ccggccggga gaaggcttcc gtcgcggaag cgccccggac
     4321 gaggcgttcc ccgaggaagg c
//
LOCUS       DATACA010000290         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2849108, whole genome shotgun sequence.
ACCESSION   DATACA010000290 DATACA010000000
VERSION     DATACA010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849108"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VHK28_05975"
     CDS             <1..390
                     /locus_tag="VHK28_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2141812.1"
                     /translation="AGGGTNGDRGYFYSPTIVTNVAQDAEIVQQEVFGPVVTVQRFAD
                     DEEAIRWANGVSYGLAASVWTRDVKRALNAARRLRYGTVWINDHLTIASEMPHGGFGQ
                     SGYGKDMSLYSIEDYTIIKHVMAKIAD"
     gene            complement(516..959)
                     /locus_tag="VHK28_05980"
     CDS             complement(516..959)
                     /locus_tag="VHK28_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141813.1"
                     /translation="MTLTHQDVSDPLHGLPRHPDLACTSKSGIGLRSTATCAAEIDTA
                     AIRARQFVHEPPGLAELRWETHHACGVPMTFRPPLATIVDVRAYRYGISGGYGLAWWL
                     SWRSCRTAAWRARHIGQFFTRGVGCVSICRYSGRAAARTDRIFGP"
     gene            1082..1435
                     /locus_tag="VHK28_05985"
     CDS             1082..1435
                     /locus_tag="VHK28_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141814.1"
                     /translation="MWLLLSLTALLVIVASAIFMVGLQGITLGWPLLGLAALPVLAAV
                     LLVLRHAYAEAFLRMTAVFWAPLLIAYLFGILLGGADGPRPEWALIAGLVVLVAGAAS
                     VVALVAGHRHLRAQH"
     gene            1498..1950
                     /locus_tag="VHK28_05990"
     CDS             1498..1950
                     /locus_tag="VHK28_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX2141815.1"
                     /translation="MADASVRTDPETPGELPPGRTPALEPDEVDRLFRRGVALFNGVR
                     YWHAHEAWETLWRAASDEERDFYQGLIQVAAGLLHLQRRNMRGARNKLREGLEKLRPF
                     QPTHQGIFVNELIGRAHRILEELEGGALPYLIPPVIRYTGWDQVDFDR"
     gene            1947..3350
                     /locus_tag="VHK28_05995"
     CDS             1947..3350
                     /locus_tag="VHK28_05995"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004926328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX2141816.1"
                     /translation="MSSPREEEGFRVEHDSMGAVRVPAGARWRAQTQRAVENFPVSGL
                     RLESAHVRALGLIKAAAASVNAELGVLEPEMADAIRQAAAEVAQGDWDAEFPIDVFQT
                     GSGTSSNMNANEVIASIAAERLGSPVHPNDHVNASQSSNDVFPSSIHLAATDAVANDL
                     IPALEHLAVALEAKRDEFASVVKAGRTHLMDATPVTLGQEFGGYADQVRKGIERLNAS
                     LPRLAELPLGGTAVGTGINTPPGFAQRVIAQLRQETGLPLTEARDHFEAQGARDGLVE
                     ASGQLRTIAIGLYKVSNDLRWMGSGPRAGLAEINLPDLQPGSSIMPGKVNPVIPEAMC
                     MVCAQVIGNDATVAFAGSAGNFELNVMLPVMARNLLESIRLLANMSRLFADRCVSGIT
                     ANVERAREYAESSTSIVTPLNRYIGYEEAAKVAKQSLAERKTIREVVIERGYVRDGKL
                     SEAQLDEALDVLRMTRP"
     gene            3617..5116
                     /locus_tag="VHK28_06000"
     CDS             3617..5116
                     /locus_tag="VHK28_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2141817.1"
                     /translation="MTANRNLIGDRWVPARSGATFVSVNPANHDDVIGEFAASGADDV
                     DAAVQAARAAYPAWSLMPAPKRGEILFRVARLLAEHKEELSRLMTREMGKVLPEARGD
                     VQEAIDVAYYMAGEGRRLFGQTVPSEMPDKFAMAIRRPIGVAGIITPWNFPIAIPAWK
                     LFPALICGNTAVIKPASDTPACVVRFVELLTEGGIPAGVVNVVTGSGGEVGNALVEHP
                     DVRLISFTGHTETGVEISRRAAETLKRVSLELGGKNPIVIWEDADLDLALDSVVWSAF
                     GTSGQRCTAASRIVVHRSIHDRFVEALRGRVAKLVLGDGLLETTDVGPVINDRAVERI
                     ASFAAVGRGEGELVIGGEPARDGDLARGSFFQQTIYADVRPAARIAQEEIFGPVTSVI
                     PVDSWEETVEVVNGVKYGLSTSLFTRDINLAFRSIRDFESGLGYVNHGTIGAEAHLPF
                     GGTKATGNGHREVGQAALEFFSEWKSVYIDYSGKLQRAQIDTTFVDQEP"
BASE COUNT          819 a         1774 c         1796 g          785 t
ORIGIN      
        1 gccggtggcg ggaccaacgg ggatcgcggc tacttctact cgccaaccat cgtgaccaat
       61 gtcgcccagg acgccgagat cgtgcagcag gaggtcttcg gtccggtcgt caccgtgcag
      121 cgcttcgccg acgacgagga ggccatccgc tgggccaacg gcgtgagcta cgggctggcg
      181 gcatcggtct ggacgcggga cgtgaagcgg gcgctcaacg ccgcgcgtcg gctgcggtac
      241 gggacggtgt ggatcaacga tcacctgacc atcgcgtccg agatgccgca cggcggattc
      301 gggcagtccg gctacggcaa ggacatgagc ctgtacagca tcgaggacta caccatcatc
      361 aagcacgtca tggccaagat cgccgactga ccgcctgtca ggttcttgga cgatcggccg
      421 cgccggtacg gccgcggtgc cgattcagcg ggcagccgtt cacgggacgc agccgccgcg
      481 tgccgttctg ccgttccatc gggcagccgt gagtgtcagg ggccaaagat cctgtcggtt
      541 cgtgctgcgg cccgcccgct gtatcggcag attgacacgc agcccacgcc ccgcgtgaag
      601 aactgcccga tatggcgggc acgccacgcg gcggtgcggc agcttctcca cgatagccac
      661 caggccaggc catatcctcc ggaaatgccg taccggtaag ccctaacatc gacgatggtg
      721 gccagcggcg gccggaacgt catcggtacg ccgcacgcgt gatgcgtttc ccaacgaagc
      781 tcggctaagc cgggaggttc gtggacgaac tgccgcgccc gtatggccgc ggtgtcgatc
      841 tcggcggcac aggtggcggt acttcgcaag ccgatgccgc tcttcgacgt gcacgcgaga
      901 tcggggtgac gcggcaggcc gtgcagcgga tcgctgacgt cctggtgggt cagggtcatg
      961 ccgagtaccg gcccaacccg gcgcaccgca aggcaaagct actcgcgccg acggctctcg
     1021 gtcgcgaggc ggtacgcagc atcgctccgg gctacgctgc ctttgcgggt agtctggccg
     1081 catgtggctg ctgctttcgc tgacggcgct gctggtgatc gtggcctcgg cgatcttcat
     1141 ggtcggcctg caggggatca ccctgggatg gccgctgctg gggctcgcgg ccctcccggt
     1201 cctggcggcc gtgctgctgg tcctgaggca tgcctacgcc gaggcattcc tgcgcatgac
     1261 ggccgtcttc tgggcgccgc tgctgatcgc ctacctcttc gggatcctgc ttggtggcgc
     1321 ggacggcccg cgccccgaat gggcgctcat cgcgggcctg gtggtcctgg tggccggagc
     1381 ggccagcgtg gtggccctgg ttgccggcca ccggcacctg cgggcgcagc actgatcgac
     1441 gggccgtacc ctcatgctgc ccgcttgtca gcggggcgcg cctgctagga tcgggcagtg
     1501 gcagacgcat ccgtccgcac cgatccggag accccgggcg agctgccgcc cggacgcacg
     1561 ccggcactcg aaccagacga ggtcgaccgg ctcttccgac gcggcgtggc gctcttcaac
     1621 ggcgtgcggt actggcatgc tcacgaggcc tgggagacgt tatggcgagc cgcatccgac
     1681 gaggaacgcg acttctacca ggggctgatc caggtcgcgg ccggactgct gcatctgcaa
     1741 cgtcgcaaca tgcgcggcgc gcgcaacaag ctgcgcgagg ggctcgagaa gctgcgtccc
     1801 ttccagccca cccatcaggg catcttcgtc aacgagctga tcggccgcgc ccaccgcatc
     1861 ctggaggagc tggagggtgg cgccctgccg tacctgatcc ccccggtcat ccgctacaca
     1921 gggtgggacc aggtcgactt cgaccgatga gctcgccacg agaagaggag ggcttccgcg
     1981 tggagcacga ctcgatgggc gcggtgcgcg ttccggccgg cgctcggtgg cgagcccaga
     2041 ctcagcgcgc ggtcgagaac ttcccggtgt ccggactgcg gctggaatcc gcccacgtgc
     2101 gggccctggg gctgatcaag gcggcggcag catcggtcaa tgccgagctt ggcgtcctcg
     2161 agcccgagat ggccgatgcc atccgccagg ccgccgccga ggtcgcgcaa ggcgactggg
     2221 acgccgagtt cccgatcgat gtcttccaga ccgggtccgg cacgagctcg aacatgaatg
     2281 ccaacgaggt gatcgccagc attgccgccg aacgactcgg atcgccggtt caccccaacg
     2341 accacgtgaa cgccagccag tcgagcaacg acgtcttccc gtcgtccatc cacctggccg
     2401 caaccgatgc agtagccaac gacctcatcc cggccctgga gcacctggcg gtcgccctcg
     2461 aggccaagcg cgacgagttc gcatcggtgg tcaaggccgg gcgcacgcac ctgatggacg
     2521 ccacgccggt cacccttggc caggagttcg ggggctacgc cgaccaggtg cgcaagggga
     2581 tcgagcggct gaacgccagc ctcccgcgcc tggccgagct gccgctgggc gggacggcag
     2641 tggggaccgg catcaatacg ccgcccggct tcgcccaacg ggtgatcgcg cagctgcggc
     2701 aggagaccgg ccttcccttg accgaggcac gcgaccactt cgaggcacag ggtgcgcgtg
     2761 acgggctggt cgaggcctcc ggccagctgc ggacgatcgc gatcgggctc tacaaggtca
     2821 gcaacgacct gcgctggatg ggctccggcc cgcgagccgg gctggccgag atcaacctgc
     2881 cggacctgca gcccggctcg tcgatcatgc cgggcaaggt gaacccggtg attcccgagg
     2941 cgatgtgcat ggtctgcgcc caggtgatcg gcaacgacgc gaccgtggcc ttcgccggct
     3001 cggcggggaa cttcgagctg aacgtcatgc tgccggtcat ggcccgcaac ctcctggagt
     3061 cgatccgcct gctggccaac atgtctcgcc tgttcgcgga ccgatgcgtc agcggcatca
     3121 ccgccaacgt ggagcgcgcc cgcgaatacg cggagagctc cacctccatc gtcacgccgc
     3181 tgaaccgcta catcggctac gaggaggcgg caaaggtggc caagcagtct ctggccgagc
     3241 gcaagacgat ccgcgaagtc gtcatcgagc gcggctacgt gcgggacggg aagctgagcg
     3301 aggcgcagct cgacgaggcg ctcgacgtct tgcgcatgac gcggccctag cgcatgaaga
     3361 cgcgccggcc cgttgccgga ccggcgcgac acgacgtccg tcacgatggg tgtgccacta
     3421 cttcagcccc gtgaacgtac gtttacggaa cgcccaccgc gacggctgtt cggttgtccc
     3481 gccatcctcg ccgccgtggc gccggaagga ctaggggcga atgtcccgcg acagccggga
     3541 atcgaacccc gcgtacaatc gggccgccat ccataccgcc cctgagtcgg ctgccgcccg
     3601 aggagaatct ctgccagtga ccgccaaccg caacctgatc ggagaccgat gggtccccgc
     3661 ccgctccggg gccaccttcg tgagcgtcaa cccggccaac cacgacgacg tcatcggcga
     3721 gttcgcagcc tcgggtgcgg atgacgtgga cgccgcggtg caggctgccc gggctgccta
     3781 tccggcctgg tccctgatgc cggcgcccaa gcgcggcgag atcctgttcc gcgtcgctcg
     3841 cctgctggcc gagcacaagg aggagctgtc tcgcctgatg acgcgcgaga tgggcaaggt
     3901 gctccccgag gcgcgcggtg acgtgcagga ggcgatcgac gtcgcctact acatggccgg
     3961 cgaaggccgg cgcctgttcg gccagacggt gccgtccgag atgcccgaca agttcgccat
     4021 ggcaatccgc cgcccgatcg gcgtcgccgg catcatcacc ccctggaact tcccgatcgc
     4081 catcccggcc tggaagctct tcccggcgct gatctgcggc aacaccgcgg tcatcaagcc
     4141 cgcctccgac accccggcct gcgtggtgcg cttcgtcgag ctgctgaccg agggcggcat
     4201 cccggctggc gtggtcaacg tggtgaccgg cagcggcggc gaggtcggga atgccctggt
     4261 cgagcatccc gacgttcggc tcatcagctt caccggccac accgagaccg gcgtcgagat
     4321 cagccgccgg gcggcggaga cgctcaagcg cgtcagcctg gagctgggcg gcaagaaccc
     4381 gatcgtgatc tgggaggatg cggatctcga cctggcgctg gacagcgtgg tgtggtccgc
     4441 gttcggcacc agcggccagc gctgcacggc agccagccgg atcgtggtgc atcggtccat
     4501 ccatgaccgc ttcgtcgagg cgctgcgcgg ccgggtggcg aagctggtgc tcggggacgg
     4561 gctgctggag acgaccgatg tcgggccggt catcaacgac cgcgcggtcg agcgcatcgc
     4621 ctccttcgca gcggtcggac gcggcgaggg cgagctggtg atcggtggcg agccggcgcg
     4681 cgacggcgac ctggccaggg ggtcgttctt ccagcagacg atctacgccg acgtgcggcc
     4741 ggcggcgcgg atcgcgcagg aggagatttt cggtccggtc acctcggtca tcccggtgga
     4801 cagctgggag gagacggtgg aggtcgtgaa cggtgtgaag tacgggctct cgacctcgct
     4861 gttcacgcgc gacatcaacc tggccttccg ctccatccgt gacttcgagt cgggcctcgg
     4921 ttacgtgaac cacggcacca tcggcgccga ggcgcacctg ccgttcggcg ggaccaaggc
     4981 caccggcaac ggccaccgcg aggtgggaca ggccgcgctg gagttcttca gcgagtggaa
     5041 gagcgtctac atcgactact ccgggaagct gcagcgcgcc cagatcgaca ccaccttcgt
     5101 cgaccaggag ccctaggccg gcgtcgcacc tgcccggctt gcctccccgt cggcgggatg
     5161 ggacgatgcc ggct
//
LOCUS       DATACA010000291         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2206075, whole genome shotgun sequence.
ACCESSION   DATACA010000291 DATACA010000000
VERSION     DATACA010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206075"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..246)
                     /locus_tag="VHK28_06005"
                     /pseudo
     CDS             complement(1..246)
                     /locus_tag="VHK28_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092182.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(254..1386)
                     /locus_tag="VHK28_06010"
     CDS             complement(join(254..954,954..1386))
                     /locus_tag="VHK28_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611506.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2141818.1"
                     /translation="MKRYRVTLTALEREELGRLLARGKAGVRKLKHAQVLLKADEAEA
                     GPGWADERIAEALEVGLATVQRLRRRFVEEGLAAALSPYRGGKRIYATKLDGEQEAHL
                     IALACSSPPEGHSRWTLRLLAGQMVELQHVDALSHETVRQTLKKNALKPHLRRMWCIP
                     PQHSAAFVAHMEDVLAVYQRAYDPKHPMVCLDETSKQLIGEVRAPLPSAPGQIARYDG
                     EYVRNGTANIFMAFEPLAGWRQARVTDQRCRRDWAAFVKELVDGRYKDAATIVLVMDQ
                     LNTHTPASLYEAFPPEEARRLAEKLEIHHTPKHGSWLNMAEIELSVLSRDLPDRIGDK
                     AAIEQHVAAWEQRRNTARVKANWQFTTNDARIKLRKLYPSLQA"
BASE COUNT          289 a          590 c          541 g          295 t
ORIGIN      
        1 gaccgcgaag gcgtcctgca gaggcaggct atcggccgcc gcctgatggc tgaagtcggt
       61 ctcactcagc accacgccag cgaagaaggc gccgagcgcg aaggagacgc caaacagctc
      121 ggccgagccg taagcgatgc cgagcgccgt ggccagcacg gacaacgtaa acagctcgcg
      181 cgatcccgtg cgcgctgcct gctccagcac ccagggcacg acgcgcgtgc cgacgatcag
      241 cactagtgct ccgtcaggct tgaagtgagg gatagagctt tcgcagcttg atgcgagcat
      301 cgttggtggt gaactgccag ttggccttga cccgggccgt gttgcggcgc tgctcccagg
      361 cggcgacatg ctgctcaatc gctgccttgt cgccgatccg gtcgggcagg tcgcggctga
      421 ggacgctgag ctcgatctcg gccatgttga gccaagaccc atgcttgggt gtgtggtgga
      481 tctcaagctt ctcggccagg cgtctggcct cctcgggcgg gaaggcctcg tagagcgaag
      541 cgggtgtatg ggtgttgagc tggtccatga cgaggacgat tgtggccgca tccttgtagc
      601 gtccgtccac cagctccttg acgaacgctg cccaatcccg gcggcagcgc tggtcggtca
      661 cccgtgcctg gcgccagccg gccaggggct cgaacgccat gaagatgttg gcggtgccgt
      721 tccggacata ctcgccgtca taacgagcga tctggcccgg tgccgatggc agtggcgcac
      781 gaacctcgcc gatcagctgc ttgctggtct cgtccaggca gaccatcgga tgcttcggat
      841 cgtaagctcg ctggtagacg gccagcacgt cctccatgtg ggcgacgaac gcggctgagt
      901 gctggggtgg gatgcaccac atccgcctca gatgcggctt gagcgcgttt ttttgagcgt
      961 ctgccgcacc gtctcgtgcg acagcgcgtc gacgtgctgg agttcgacca tctgcccggc
     1021 caagagccgc agcgtccagc ggctatgccc ctcgggcggt gaggagcaag ccagtgcgat
     1081 cagatgcgct tcctgctccc catcgagctt ggtggcgtag atgcgcttgc cgccacgata
     1141 ggggctgagt gccgccgcta acccctcctc gacaaatcgc cgccggagac gctgcacggt
     1201 ggcaaggcca acctccagcg cttcggcaat gcgctcgtcc gcccagcccg gtccagcctc
     1261 ggcctcgtcc gccttgagca ggacctgcgc gtgtttgagc ttgcggacac cggccttgcc
     1321 ccgcgccagc agccgcccaa gctcctcccg ctccagcgcc gtcagcgtca cgcgataccg
     1381 cttcatccgc cgcctcctgc cagtccacaa tggaccaacg gcgcagcagg ttcatcagat
     1441 catcgctgac agagcactag cctgttgagg tacgagaatc gttacccgcc ggactggctt
     1501 tgcagaaccc gcgcagaaca ggcagcctcg gagaacctgc aaaggatcac gatcaggtcc
     1561 gccggccgaa ctgcaaccca agcgtcggcc agcgccgcgt cggccctcct gtccaggtct
     1621 tccccagctc tcgcttcaac gaccagcacc cgcggactcc ggcctcggtc gagcacccgc
     1681 tccaaccgtt gcaggcgccc ctccatggct ctcac
//
LOCUS       DATACA010000292         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2366489, whole genome shotgun sequence.
ACCESSION   DATACA010000292 DATACA010000000
VERSION     DATACA010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2366489"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VHK28_06015"
     CDS             <1..195
                     /locus_tag="VHK28_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2141819.1"
                     /translation="TMGLFLLVINALMLLLTGLISDQLDLGLQVADFWAALLGSIVIS
                     LVGWVLSMVVGAARMPGKVL"
     gene            218..895
                     /locus_tag="VHK28_06020"
     CDS             218..895
                     /locus_tag="VHK28_06020"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEX2141820.1"
                     /translation="MSADRDHPLVAFVFAATVALAILLTGPMLLFAPPIVSFMQARHD
                     VAARLETDQATVDRATGVMLRDLLLDGDFTVSIRDSGEPVLDPSERSHMRDVGAVVRT
                     LLLIDVVALVVVALAAGRLRRDPARRGRLVIMAAAGIGLAALVLAAFFAFAFDVAFAA
                     FHALFFDPGTWQFGPDSNLLRFFPQPFWFEIALVAGGTIVLGALLASALAWRDIAGSH
                     RRPPVPG"
     gene            917..>2029
                     /locus_tag="VHK28_06025"
     CDS             917..>2029
                     /locus_tag="VHK28_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2141821.1"
                     /translation="MGRGSFSIGSIAGIRIALHPSWLVIAFLVTYSLAVGDLPDRFPL
                     WEPALYWAVAAGVAALFFASVLAHELSHALVARRFDIKVSDITLFVFGGAASLEGDAR
                     TPRQEALIAFAGPLCSLLIGGALYGVDVLVGQPQVAALVAWLAFINLSLGIFNLFPGF
                     PMDGGRLLRALLWKVRGDQFSATRSAAMVGRGFGYLLIAGGVFIAFQTGGLFSGIWLA
                     LIGWFITTAAEATVAQVSVQRTLRGVRVRDVMEPEPASVSPNEPVVELVNERLMRGDG
                     RSFLVRHDDGGLAGIVTLTDVRRVSREEWSHARVTDIMTRFNDLVVARPEDELDDALE
                     RLQAREVNQLPVVDEDGRTVVGLLTRAGILRLIDTRM"
BASE COUNT          220 a          725 c          709 g          375 t
ORIGIN      
        1 accatggggc tgttcctgct ggtcatcaac gcgctgatgc tgctgctcac cgggttgatc
       61 agcgaccagc tggacctggg cctgcaggtc gccgacttct gggcggcgct gctgggctcg
      121 atcgtgatct cgctggtcgg ctgggtcctc tccatggtgg tcggcgcggc acgcatgccg
      181 ggtaaggtcc tctagcctgc cggaccttcc gggcccgatg tccgcggacc gcgaccaccc
      241 cctggtcgcc ttcgtctttg cggcgaccgt ggccctggcc atcctgctga caggaccgat
      301 gctgctcttc gccccgccga tcgtttcgtt catgcaggcg cggcacgatg tggccgcgcg
      361 cctggagacc gaccaggcca ccgtggaccg cgccaccggc gtcatgctcc gcgacctgct
      421 cctcgacggc gacttcacgg tcagcattcg cgactcgggt gagccggtcc tcgacccctc
      481 agagcgcagc cacatgcgag acgtgggggc cgtggtgcgg accctgctgc tgatcgacgt
      541 cgtcgcgctg gtggtggtgg cgctcgccgc tggacgcctg cgacgcgatc ctgcccggcg
      601 cggccgcctg gtgatcatgg ctgcggccgg catcgggctg gcggcgctcg tgctggccgc
      661 cttcttcgcg ttcgccttcg acgtggcctt cgccgccttc cacgcgctgt tcttcgatcc
      721 cggcacgtgg cagttcggac cggactcgaa cctgctgcgc ttcttcccgc agccgttctg
      781 gttcgagatc gcgctcgtgg cgggcggcac catcgtgctc ggcgcgctgc tggcctccgc
      841 tctcgcctgg cgcgacatcg ctggttccca ccgccggccg ccagtgcccg gctgacgctc
      901 ggctacactc cggccaatgg gtcgcggctc gttctccatc ggcagcatcg cggggatccg
      961 catcgcgctc cacccctcgt ggctggtgat cgcgttcctg gtcacctact cgctggcggt
     1021 gggcgacctg ccggatcgct tcccgctgtg ggagccggcg ctgtactggg cggtggcggc
     1081 cggcgtcgcg gccctcttct tcgcctcggt cctggcccac gagctgagcc atgcgctggt
     1141 ggcgcgccga ttcgacatca aggtcagcga catcaccctg ttcgtgttcg gcggcgccgc
     1201 gtccctggag ggagacgccc ggacgccgcg gcaggaggcg ctgatcgcct ttgccggtcc
     1261 gctctgcagc ctgctgatcg gcggcgcgct gtacggcgtc gacgtcctgg tgggccagcc
     1321 ccaggtggcc gctctggtcg cctggctggc gttcatcaac ctgtcgcttg gcatcttcaa
     1381 cctgttcccg ggcttcccca tggatggcgg gcgcctgctg cgcgccctgc tgtggaaggt
     1441 gcgcggcgac cagttctcgg ccacccgcag cgcggccatg gtcggccgcg gcttcggcta
     1501 cctgctgatc gccggcgggg tgttcatcgc cttccagacc ggcggcctgt tcagcggcat
     1561 ctggctggcg ctgattggct ggttcatcac caccgcggcg gaggccaccg tggcccaggt
     1621 cagcgtgcag cgcacgctgc gtggcgtgcg ggtgcgggac gtcatggagc cggaaccggc
     1681 atcggtctcg ccgaacgagc cggtggtcga gctggtgaac gagcgactca tgcgcggcga
     1741 cggtcggtcc tttctggtcc ggcacgacga cggcgggctg gccgggatcg tgaccctcac
     1801 cgacgtgcgt cgcgtctccc gcgaggagtg gtcgcacgcc cgcgtgaccg acatcatgac
     1861 ccgcttcaac gacctggtcg tcgctcgccc cgaggacgag ctggacgacg ccttggagcg
     1921 cctccaggcc cgcgaggtca accagctgcc ggtggtggac gaggacggcc ggacggtggt
     1981 gggcctgctg acccgcgccg ggatcctgcg gctgatcgac acgcgcatg
//
LOCUS       DATACA010000293         1584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1885356, whole genome shotgun sequence.
ACCESSION   DATACA010000293 DATACA010000000
VERSION     DATACA010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1885356"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1584)
                     /locus_tag="VHK28_06030"
     CDS             complement(<1..>1584)
                     /locus_tag="VHK28_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2141822.1"
                     /translation="RLQHAMAAGFAQALRERTLAEQEAIAQAALRAQGVVAQALHATE
                     ARFRAVFEGAAIGIGMADLDGNILQVNGALLRMFGVSEQTMRSRNVMEWTHPDDAPQT
                     WRLYDELVRGEREHYHVEKAFYRPDGTVLWTNLTVSLLRDADGSPQYQLALMEDTTER
                     RLLNLRLRYEATHDALTGLPNRTFFFERLEKALGAGKGQRFGLCYLDLDGFKTINDSL
                     GHAAGDRLLVEVADRLQSCATAPGEMVARLGGDEFVALTTGPDTQREVDELATRIMNA
                     LLAPISVDGRELTVRGSIGIVEGPAGERSPAEVLRSADITMYRAKSAGGNRFELADPE
                     ADARAITRHGLTTALPTALERGEFFIEYQPLVHLGDGSVRGAEALVRWLHPQHGVLGP
                     DRFIPLAEHTGLIVPLGRWVLEQSVRQAREWRELHGGVEAAGPLRINVNLSPCQLTHP
                     GLVQDTVDILERTGISPDALCLEVTESALIGADDDLLKPLRRLAEMGVDIALDDFGTG
                     YSNLANLRRLPVSILKLDRS"
BASE COUNT          208 a          548 c          586 g          242 t
ORIGIN      
        1 ggagcggtcc agcttgagga tgctgaccgg caggcggcgc agattggcga ggttggagta
       61 gccggtgccg aagtcgtcca gggcgatgtc gacgcccatc tccgccaggc ggcgcagcgg
      121 cttcagcagg tcgtcgtccg cgccgatcag cgccgactcc gtgacctcca ggcagagcgc
      181 gtccggtgag atgcccgtgc gctccaggat gtcgacggtg tcctggacca ggccggggtg
      241 ggtcagctgg cagggcgaga ggttgacgtt gatccgcagc ggtcccgcag cctccacgcc
      301 accgtgcagt tcccgccact cgcgggcctg ccgcaccgac tgttccagga cccagcggcc
      361 caacggcacg atcagcccgg tgtgttcggc gagcgggatg aaccggtccg ggccgagcac
      421 cccgtgctgc gggtgcagcc agcgcaccag ggcctccgca ccgcgcacac tgccgtcgcc
      481 gagatggacc agcggctggt actcgatgaa gaactcgccc cgctccaggg ccgtcggcag
      541 cgccgtggtc agcccgtgcc gggtgatggc gcgcgcgtcg gcttccgggt cggcgagttc
      601 gaagcggttg ccgcccgccg acttggcccg gtacatggtg atgtcggcgc tgcgcagcac
      661 ctccgccggg ctgcgctccc cggccgggcc ctcgacgatg ccgatgctgc cgcgcacggt
      721 cagctcccgg ccgtcgacgc tgatcggcgc gagcagggcg ttcatgatgc gcgtggcgag
      781 ctcgtcgacc tcgcgctggg tgtcggggcc cgtggtcagg gccacgaact cgtcgccgcc
      841 gagccgggcg accatctcgc cgggcgcggt ggcgcaggac tggaggcggt cggcgacctc
      901 caccagcagc cggtcgccgg ccgcgtggcc gagactgtcg ttgatggtct tgaagccgtc
      961 cagatccagg tagcagaggc cgaaccgctg gcctttcccg gcgcccagtg ccttctccag
     1021 gcgctcgaag aagaacgtgc ggttcggcag cccggtcagc gcgtcgtgcg tggcctcgta
     1081 acgcagcctc aggttgagca gccgccgctc ggtggtgtcc tccatgagcg cgagctggta
     1141 ctgcggactg ccgtcggcgt cccgcagcag ggagaccgtc aggttggtcc acaggaccgt
     1201 tccgtcaggg cggtagaacg ccttctccac gtggtagtgc tcgcgctcgc cgcgcacgag
     1261 ttcgtcgtag agccgccagg tctgaggggc gtcgtcgggg tgggtccact ccatgacgtt
     1321 gcggctgcgc atcgtctgct cggagacccc gaacatgcgc agcagtgcgc cgttgacctg
     1381 gaggatgttg ccgtcgaggt cggccatgcc gatgcctatg gccgcgccct cgaagacggc
     1441 gcggaagcgg gcctcggtgg cgtgcagggc ctgtgccacc acgccctgcg cccggagggc
     1501 ggcctgggcg atggcctcct gctcggccag cgtccgctcg cgcagcgcct gcgcgaagcc
     1561 ggcggccatc gcgtgctgga ggcg
//
LOCUS       DATACA010000294         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1804901, whole genome shotgun sequence.
ACCESSION   DATACA010000294 DATACA010000000
VERSION     DATACA010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804901"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VHK28_06035"
     CDS             <1..208
                     /locus_tag="VHK28_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141823.1"
                     /translation="VQHVEVGPWDGYGHSGLLQGFTSLLVDLPAQGVTLVVIGTSRGF
                     SPAELLSWAPKGEDSILDLALAAR"
     gene            complement(372..2051)
                     /locus_tag="VHK28_06040"
     CDS             complement(372..2051)
                     /locus_tag="VHK28_06040"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2141824.1"
                     /translation="MEFDSIVTDVVVGYLRHHPVHATDIGHHEHDGIWPDLTDAGRQE
                     RVAWVRGAIGQLEGLEAGSLTPDQTIDRRILLENLAAVLFHEETLREGSWNPLGYVYL
                     FGNGLFALLAREFAPLPERLASAASRMRGLPDALLAARATLGSGGPRPVSRFHAQKAL
                     ERADGIADLCRSALREAEGCGDEALASEVRSAAGEAEEAVTAFKSWLADELLPRVDGD
                     FRLGPELYAEKFRHALKTEVTPEDLAATASAAYEEVRAEMLRLARELWPAWIGDEPPP
                     SDPDQLVRRVLDAIAADHPRAEELLDFCRAENERIEAFVRERDLIGLADEPLKIIWTP
                     EFLRSSGGAMLIPPGPLDRGLDSFFAITPMPDDWTDERKESYLREDNARMLRLLTIHE
                     AVPGHYLQLAYSNRCDSLARTIFQSGVFAEGWAVYVTQVMMDVGYGAEDPALQLVHWK
                     FFLRSITNTLMDIRIHTGSMDEREAMQLMVEGGFQEEGEAAAKWDRARLSSTQLCEYF
                     LGSVLMHELEAEARRRASAEGREFAYRPFLESVLAHGTPSMPVIRDILFAG"
     gene            2108..>2256
                     /locus_tag="VHK28_06045"
     CDS             2108..>2256
                     /locus_tag="VHK28_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX2141825.1"
                     /translation="MGKRERRKAETREALIDAAARLFAEQGVEGTTMDDIARAAGTSR
                     TSVFN"
BASE COUNT          339 a          803 c          777 g          337 t
ORIGIN      
        1 ggtgcagcac gtcgaggtcg gcccgtggga cggctacggc cactccgggc tgctgcaggg
       61 attcacctcc ctgctggtcg acctgcccgc ccagggcgtg acgctggtgg tcatcggcac
      121 cagccgcggc ttttccccgg cagagctgct cagctgggcg ccgaagggcg aggattcgat
      181 cctggacctc gcactcgccg cgcggtaggg gcctcacacc ctggctgggt gacacgaccg
      241 atgacgaccg ggcgcgcgga cgccctccga cgtgtcacac gccagttgcg tgctgggatc
      301 gataaacacc tgcgaccgat agctcggcgg acgcctcacc cggccgctgc gtaggcccga
      361 cccccggcca cctacccggc gaagaggatg tcgcggatga ccggcatgga cggcgtgccg
      421 tgggccagga ccgactccag gaaggggcgg taggcgaact ctcggccctc agcggacgcc
      481 cggcgccgcg cctccgcctc cagctcgtgc atgaggacgc tgcccaggaa gtattcgcat
      541 agctgcgtcg acgacagcct ggcccggtcc cacttggccg ccgcttcgcc ctcctcctgg
      601 aagccaccct ccaccatgag ctgcatcgcc tcgcgctcgt ccatcgagcc ggtgtggatg
      661 cggatgtcca tgagcgtgtt ggtgatgctc cgcaagaaga atttccagtg gaccagctgc
      721 agggccggat cctccgctcc gtagccgacg tccatcatca cctgggtcac gtacaccgcc
      781 cagccctcgg cgaagacgcc cgactggaag atggtccgcg ccagcgaatc gcatcggttc
      841 gaataggcga gctgcaggta gtgcccgggg accgcctcgt ggatggtgag caggcgcagc
      901 atccgggcgt tgtcctcgcg caggtaggac tccttgcgct catcggtcca gtcgtctggc
      961 atgggggtga tggcgaagaa gctgtccagc ccgcggtcca gcgggccggg cgggatgagc
     1021 atggcgccac ctgacgagcg gaggaattcg ggggtccaga tgatcttcaa cggctcgtcg
     1081 gccagaccga tgaggtcgcg ctcccgcacg aacgcctcga tccgctcgtt ctccgcgcgg
     1141 cagaagtcga gcagctcctc cgcccgcggg tgatcggcgg cgatggcgtc caacactcgg
     1201 cgcaccagct ggtcggggtc tgatggcggc ggctcgtccc cgatccaggc cggccacagc
     1261 tcgcgcgcca ggcgcagcat ttcggcgcgc acctcctcgt aggcggccga tgcggtggcg
     1321 gccaggtcct cgggcgtgac ctcggtcttg agcgcgtgcc ggaacttctc ggcgtacagc
     1381 tccggcccca gccggaagtc gccgtccacc cgcggcagga gctcatcggc cagccaggac
     1441 ttgaaggccg tgacggcctc ctccgcctca ccagcggcgc tccgcacctc gctggccagg
     1501 gcctcgtctc cgcatccctc cgcctcccgg agggcgctgc ggcacaggtc ggcgatgccg
     1561 tcggcgcgct ccagggcctt ctgcgcgtgg aagcggctga ccggtcgcgg cccgccggac
     1621 ccgagcgtgg cccgcgcggc cagcagtgcg tccggcaggc cgcgcatgcg cgatgccgcg
     1681 ctggcaagac gctcgggcag cggcgcgaac tcgcgcgcga gcagcgcgaa caggccgttc
     1741 ccgaacaggt acacgtagcc cagcgggttc cagcttccct cccgcagggt ctcctcgtgg
     1801 aacagcacgg ccgccaggtt ctcgagcagg atgcggcggt cgatcgtctg gtccggcgtg
     1861 agcgaccccg cctcgagccc ctccagttgc ccgatcgcgc cgcgcaccca ggccacccgc
     1921 tcctggcggc cggcatcggt caggtccggc cagatgccat cgtgctcatg gtgaccgatg
     1981 tcggtggcgt gcaccgggtg atggcggagg taaccgacca ccacatcggt cacgatgctg
     2041 tcgaattcca tgccggcatg ctagcaattg ctgaaggcgg ccagcggtct gctagcctgt
     2101 atgggcggtg ggcaaacgcg agcgcaggaa ggcggagacg cgggaggcgc tgatcgatgc
     2161 cgccgcccgc ctcttcgccg agcagggcgt cgaaggcacc acgatggatg acatcgcgcg
     2221 tgccgccggc acgagccgca cgagcgtctt caacta
//
LOCUS       DATACA010000295         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_518949, whole genome shotgun sequence.
ACCESSION   DATACA010000295 DATACA010000000
VERSION     DATACA010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518949"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1044)
                     /locus_tag="VHK28_06050"
                     /pseudo
     CDS             complement(1..1044)
                     /locus_tag="VHK28_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276282.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
     gene            complement(1064..1918)
                     /locus_tag="VHK28_06055"
     CDS             complement(1064..1918)
                     /locus_tag="VHK28_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX2141826.1"
                     /translation="MNDRFCPNCGTEVDEDARFCPTCGQTLLLDEDAPVEAPGGRASF
                     QPDLPDAPAWPEPEPEPAPEPEPEPERPAEADSPLPTGDDEPTRTTSVNAGGASAEWP
                     REAEPPARGEPTEDEGYQTAVPPPPLTPLAGTTTAPPADSEALGRTEEPTGERAGARS
                     RGADLPFTWPSTLGGWLVGAGSLAGAIFLIPVLDVVLNVLVFLALLAVAATVFLADRV
                     PDVPRIRLLTLSIVLIALGIALDRAAFAVRGVESLFLIAMLAAAGGVLLVELDRDRPV
                     PPPGRSTG"
BASE COUNT          283 a          700 c          720 g          264 t
ORIGIN      
        1 ccaccgcgtg cagctcgtag tgggcgaatg cacgttgcac ctcctcctcg cggccggccc
       61 gcacgacaag gagcatgcgc tcctgcgact cggacagcag cagctcgtag gcgcccaggc
      121 ccggctcgcg ggtggggacc aggcccaggt ccacctccgc gccgcagccg cgcgcgccga
      181 aagctcggcc agcgcacagg tcagcccggc agcgcccagg tcctgcatgg cgatcaccgc
      241 atccgagccg gagagctcca ggcaggcctc catcagaagc ttctccagga agggattgcc
      301 gacctgcacc gccgggcggc gctcgtcgcg atcttcgtcg agggaggcgg aggcgaacga
      361 cgccccctgg atcccgtcgc ggccggtggc cgaaccaacc agcagcaggc tgttgcccgg
      421 cccggcggcg cgggccagcg tgatgtcgtc atgccccgcc aggccgacgc acatggcgtt
      481 gaccagcgga ttgccgctgt agctggcgtc gaaggcgagc tccccgccga cgtcggggat
      541 gccgatgcag tttccgtatc ccgcgatccc ggccacgaca ccgcccatga ggtagcgatt
      601 gcgggctgcc gccgctccat cggtccgggc ggccgaatcg tcagcggggg acaggggtcc
      661 gaagcgcagg gagttcagga gggcgatcgg gcgggccccc atggtgaaga tgtcgcggat
      721 gatgccgccc accccggtgg cagcgccctg gtacggctcc acggcgctcg ggtggttgtg
      781 cgactcgacc ttgaagacca cggccatccc gtcgccgatg tccagcgctc ccgcgttctc
      841 accgggtccc accaggacgc gctccccgct ggtggggagc gatccgagca acggccgcga
      901 gtgcttgtag gcgcagtgct cggaccacat ggcgccgaac attcccagct cgacctcgtt
      961 cggctcgcgg cccagcgcgc gcaccaccag gccgtactcc tcctcggtca gcccgtggcg
     1021 gttgggtggc gcggcgatgc tcacccctcg gcgctcgcga tggtcagccc gtggagcggc
     1081 cgggtggcgg cactggtcgg tcgcggtcga gctcgacgag cagcacgccc ccggcggcgg
     1141 ccagcatggc gatcaggaag agagactcaa caccgcggac cgcgaaggcg gctcggtcga
     1201 gggcgatccc cagtgcgatc agcacgatgc tgagcgtcag caggcggatg cgcgggacgt
     1261 ccggcacccg gtcggccaga aaaacggtgg cagccaccgc cagcagcgcc aggaagacca
     1321 ggacgttgag caccacatcc agcaccggga tgaggaagat ggccccggcc aggctgccgg
     1381 cgcccaccag ccagccgcca agggtggagg gccaggtgaa cggcaggtcc gctcctcgcg
     1441 agcgggcccc ggcccgctca ccggttggct cctcggtcct gccgagggct tcggagtcgg
     1501 cgggcggggc ggtggtcgtg ccggccagcg gcgtcagcgg cggtggtggg acggccgtct
     1561 ggtagccctc gtcctcggtt ggctccccgc gtgcgggcgg ctccgcctct cggggccact
     1621 cggccgacgc accgccggcg ttgacgctgg tggtgcgcgt cggctcgtcg tcgccggtgg
     1681 ggagcggtga gtccgcctct gccggtcgct ccggctcggg ctcgggctcg ggcgccggct
     1741 ctggctctgg ctccggccag gccggcgcat ccggcaggtc aggctggaaa gaagctcgac
     1801 caccaggggc ttcaacgggc gcgtcttcgt caagcagcag ggtctggccg cacgtcgggc
     1861 agaagcgcgc atcttcgtcg acctcggtcc cgcagttggg gcagaagcga tcgttcacgg
     1921 ctgggtcacg acctgccgaa gcgggtgagc gccccgatca gcacgat
//
LOCUS       DATACA010000296         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1483348, whole genome shotgun sequence.
ACCESSION   DATACA010000296 DATACA010000000
VERSION     DATACA010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483348"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VHK28_06060"
     CDS             <1..301
                     /locus_tag="VHK28_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2141827.1"
                     /translation="MPNPKSGTVTFDIERAVGEIKSGRIEFKVDRAGIVHAPVGRASF
                     RPDQLTENVATLVDAINRAKPSGAKGTYMRTLTLAPTMGPGVRIDIPSALAAAAS"
     gene            530..1066
                     /gene="rplJ"
                     /locus_tag="VHK28_06065"
     CDS             530..1066
                     /gene="rplJ"
                     /locus_tag="VHK28_06065"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX2141828.1"
                     /translation="MPTEAKREAVTELAEFLRGATALAVADYRGLSVADMQSVRRTLR
                     SGGVELRIAKNRLLKIAADEAELAELKPLLNGPTAVATISGDEVALAKALQDAFRPYA
                     RTVTVRGGLLGRVPVDGPGLVRLASLPGREVLLGKFAGGIASPITGFAGVLAANLRNL
                     AGVLSAVADQKQQTEGAA"
     gene            1132..1515
                     /gene="rplL"
                     /locus_tag="VHK28_06070"
     CDS             1132..1515
                     /gene="rplL"
                     /locus_tag="VHK28_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX2141829.1"
                     /translation="MGKDEILDAISNMTVLELSELVKAVEDKFGVTAAAPVAVAAAPA
                     AGAGDGAAAATEEQTEFDAVLTDVGANKILVIKAVRELTGLGLKEAKDLVDAAPKPVK
                     EGVAKEEAEQVKEKLSEAGATVEVR"
     gene            complement(1606..1962)
                     /locus_tag="VHK28_06075"
     CDS             complement(1606..1962)
                     /locus_tag="VHK28_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2141830.1"
                     /translation="MSNHPVLVVDDDAAIREAVRDVLEQEGLPVETASDGAEALTKID
                     SRMPRLVLLDMRMPGMDGWSFARELRGAGIELPVVVMTAAADARRWAAEIGAIGVVPK
                     PFGVSELVDAVREHSS"
     gene            complement(1962..>3259)
                     /locus_tag="VHK28_06080"
     CDS             complement(1962..>3259)
                     /locus_tag="VHK28_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX2141831.1"
                     /translation="APPALLQRAWQEPVLRRFLAETPELVQLTSVDDIVDRIQERARA
                     LTGADGAIIGLWDAGREKLVFRDPSGGLRFQDPGEWIGGRAFQSQEVRFTSVAEKDAP
                     EHAEEYRTANIHAIVAAPITAEDRRLGVLIVYAARSFVFTDDVVSTTKLVAEQAAMVI
                     HSHQLAREAAEVRALAEVTRLKQDFLSVLAHDVRTPLTTVLINAELLERAIGDDPVSQ
                     RRVASLRSEASRLKLLVEEYLEVVQTEEGERELRLARHDLAELVTQAVEAVANGRTRV
                     RVVASEPVAGMYDTARIYQLVQNLVSNALKYSEVDQPVEVRVWAEGDLAHLSVSDRGM
                     GIAEADLPRVFERFHRGSNTDDRRHAGLGLGLYICRLVARDHGGDITVSSRLGEGTTF
                     TVTLKRAEPVEWSAPEPTARAEPAGGMVEPMLDPSPEST"
BASE COUNT          514 a         1160 c         1132 g          453 t
ORIGIN      
        1 gatgccgaat cccaagtcgg ggacggtgac cttcgacatc gagcgggcgg tgggggagat
       61 caagtccggt cgcatcgagt tcaaggtgga ccgcgccggg atcgtccacg ctccggtcgg
      121 ccgcgccagc ttccgcccgg accagctgac ggagaacgtc gccaccttgg tggatgcgat
      181 caaccgagcc aagccgtccg gcgccaaggg gacgtacatg cggacgctga ccctggcgcc
      241 gaccatgggc cccggcgtgc ggatcgacat cccatcagcc ctggccgcag cggcgtcgta
      301 gggcgtttgc cgtcgaggcg ctgaccggct accatcggcg accgtaactg aagaaccaca
      361 gccacagaca gcgatgcgta ccgggtgaga gccccggacc aattgccggc ccacgagagg
      421 ccggcggcac gccgaggcgc gaaccatgat cgagcggcat ctgcatcggt cctcgtgctc
      481 cggcgcgcga ggaccttttt cctgccccga acaggaggcg atagaagaga tgccaaccga
      541 agcgaagcgc gaggcggtca ccgagctcgc cgagttcctc cgcggcgcca cggcactggc
      601 ggtggccgac taccgcgggc tgagcgtggc cgacatgcag agcgtgcgtc gcaccctgcg
      661 atccggcggc gtggagctgc ggatcgccaa gaaccgcttg ctgaagatcg cggccgacga
      721 ggctgagctc gccgagctca agccgctgct caacgggccg accgccgtgg ccaccatcag
      781 cggcgacgag gtcgccctgg ccaaggcgct gcaggatgcc tttcgcccct acgcgcgcac
      841 cgtgacggtg cgtggcggcc tgctgggccg cgtcccggtg gacggacccg gcctcgtgcg
      901 gctggccagc ctgcccggcc gcgaggtgct gctgggcaag ttcgccggcg gcatcgccag
      961 ccccatcact ggctttgccg gagtgctggc ggccaacctc cgcaacctgg ccggcgtgct
     1021 gagcgccgtg gccgatcaga agcagcagac cgaaggcgcc gcctgacggc gcgcgtacat
     1081 caaccacagc gtccggataa ggagcaagca gaaccgatgg caaccaagac cctgggcaag
     1141 gacgagatcc tcgacgcgat ctccaacatg accgtgctcg agctcagcga gctggtgaag
     1201 gcggtcgagg acaagttcgg cgtgaccgcc gcggccccgg tggccgtcgc cgccgcgccg
     1261 gcagccggtg ccggtgatgg cgccgcagcc gccaccgagg agcagaccga gttcgatgcg
     1321 gtcctcaccg acgtgggcgc caacaagatc ctggtcatca aggccgtccg tgagctgacc
     1381 ggcctgggcc tcaaggaggc gaaggacctc gtggacgcgg cgccgaagcc ggtcaaggag
     1441 ggggtcgcca aggaggaggc cgagcaggtc aaggagaagc tctccgaggc tggcgcgacc
     1501 gtcgaggttc gctagtcgaa ctgcagacag ccagcgggcg caatcggcgc tcgcggcaac
     1561 cgatgagagc cggtccccac gggccggctc tcggcgcgtc cgggcctagc tgctgtgctc
     1621 ccgcacggca tccaccagct cgctgacccc gaagggcttg ggcaccacgc cgatcgctcc
     1681 gatctcggcc gcccagcgcc gggcgtcagc cgcggcggtc atgaccacca ccggcagttc
     1741 gatgcccgct ccgcgcagct cgcgcgcgaa gctccagccg tccatgcccg gcatccgcat
     1801 gtcgagcagg accagccgcg gcatccggga gtcgatcttg gtcagcgcct ccgccccgtc
     1861 cgaggcggtc tccacgggca ggccttcctg ctcgagcacg tcgcggaccg cttcgcggat
     1921 cgcggcgtcg tcgtcgacca cgaggaccgg gtggttcgac atcaggtgga ctccggcgac
     1981 ggatcgagca taggctccac catgccgccc gccggctccg ctcgcgccgt cggctcgggt
     2041 gccgaccatt cgacgggctc cgcccgcttg agggtgacgg tgaaggtggt gccctccccg
     2101 agccgcgacg agaccgtgat gtccccaccg tgatcgcggg ccaccagccg gcagatgtag
     2161 aggcccagcc ccaggcccgc gtgccggcga tcgtccgtgt tgctgccccg gtggaagcgc
     2221 tcgaacacgc gtggcagatc cgcttccgcg atgcccatgc ccctgtcgct cacgctcagg
     2281 tgggcgaggt caccctcggc ccacacccgc acctcgacgg gctggtcgac ctccgaatac
     2341 ttcagggcgt tgctgaccag gttctgcacc agctggtaga tgcgtgccgt gtcgtacatg
     2401 cctgcgacgg gctcgctggc gaccacgcgg acgcgagtgc gcccgttggc gaccgcttcc
     2461 acggcctgcg tgaccagttc ggcgaggtcg tgccgcgcga gccgcagctc gcgctcgccc
     2521 tcctcggtct ggaccacctc caggtactcc tccacgagga gcttgagcct ggatgcctcg
     2581 ctgcgaaggc tggcgacgcg acgctgcgag accgggtcgt cgccgatggc tcgctccagc
     2641 agctcggcgt tgatcagcac cgtggtcaga ggcgtgcgga catcgtgtgc cagcacggac
     2701 aggaagtcct gcttgaggcg ggtcacctcg gccagggcac gcacctcggc cgcctcgcgc
     2761 gccagctgat ggctgtggat gaccatcgct gcctgctcgg ccaccagctt ggtggtgctg
     2821 accacgtcat cggtgaagac gaacgagcgc gcggcgtaga cgatgagcac gcccagccgg
     2881 cggtcctcgg cggtgatggg cgccgccacg atggcgtgga tgttggcggt gcggtactcc
     2941 tccgcgtgct ccggagcatc cttctcggcc accgaggtga accgcacctc ctgtgactgg
     3001 aacgcgcgcc cgccgatcca ctcgccgggg tcctggaagc gcagcccgcc ggacgggtcg
     3061 cggaagacga gcttctcgcg accggcatcc cacagcccga tgatcgcccc gtcagccccg
     3121 gtcagagccc gggcccgctc ctggatgcgg tcgacgatat cgtccacgct cgtcagctgc
     3181 acgagctccg gcgtctcggc caggaagcgc cgcagcaccg gctcctgcca cgctcgctgg
     3241 agcagggcgg gaggggcga
//
LOCUS       DATACA010000297         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1965662, whole genome shotgun sequence.
ACCESSION   DATACA010000297 DATACA010000000
VERSION     DATACA010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965662"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VHK28_06085"
     CDS             <1..896
                     /locus_tag="VHK28_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2141832.1"
                     /translation="STWSDQWHALNVEVAATEVALWTADRDSARRAVERGMEVAWPAG
                     REAVIPEMELGAAELLWLGVRAEAEAVSRRALSRNDHRLEQVTRHAAELVERSTAVTA
                     AARGYGPRWQRRLGLLQQMAELEMARLTGSDAADSWAAVATGWDLLGIPYPAAYAQLR
                     QASSLLRGRARTDAAIVLLEAYARATRLGAGGLQSAISALAKRSRVELPDETDGAAEP
                     ATGPRREPAGAPAALATLTDREREVLDLVAAGLSNRDIADRLFISERTAAVHVSNVLG
                     KLGVSRRTEAAAIALSGRPPS"
     gene            1002..1181
                     /locus_tag="VHK28_06090"
     CDS             1002..1181
                     /locus_tag="VHK28_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141833.1"
                     /translation="MPTLVRVWDASRVVLPLALAVIACVALPPVPARAATPTPDAVRE
                     FLEGWASVSLPSSTS"
     gene            1368..2078
                     /locus_tag="VHK28_06095"
     CDS             1368..2078
                     /locus_tag="VHK28_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141834.1"
                     /translation="MFRPAIALLALLLLATACGGGTTPSSAASAADSTQSSAASPDTE
                     PSEVAEASVEPSAAEATACDLVPGEVAGYTLNAAGDREVTTPDSLEAEAEATEDQVLA
                     DQLRQRATFWNTMGIIESCQRSYDAEGARIIVAATRFMNEQGPEQYYASEWSQGCQPV
                     VQDFPAAPDAQLYDCPGLLPAAYAVAVVVDGTTGWEIISRLDYPTGGTMTDEMRAEML
                     ADAFVAAGTVAEVIQGDG"
BASE COUNT          312 a          721 c          734 g          354 t
ORIGIN      
        1 tctcgacgtg gagcgaccag tggcatgcgc tcaacgtcga ggtggccgcg acggaggtgg
       61 cgctctggac ggcagatcgg gactcggccc gacgcgccgt cgaacgcggg atggaggtcg
      121 cgtggccggc gggccgggag gccgtcatcc ccgaaatgga gctgggcgcc gctgagctgc
      181 tctggctcgg cgttcgagcc gaggccgagg cggtctcgcg acgcgcactc tcgcgaaatg
      241 accatcgcct tgagcaggtg actcgacatg ccgctgagct ggtggagcga tcgaccgcgg
      301 tcactgctgc cgctcgcggc tacggcccgc ggtggcagcg ccggctcggc ctgttgcagc
      361 agatggcgga gctggagatg gcgcgcctca caggtagcga cgctgcggac agctgggcgg
      421 cggtggctac gggatgggac ctgctgggca tcccgtatcc agccgcctat gcgcagctgc
      481 gccaggcgtc gtcgctgctt cggggccgag cgcggacgga cgcggccatc gtcctgctcg
      541 aggcgtatgc ccgcgccacc cggctgggtg cgggcgggct gcaatcggcc atctcggcgc
      601 tcgccaagcg gtccagggtc gagctgccgg acgagacgga cggagccgcg gaaccggcga
      661 ccggaccgcg tcgcgagccc gctggcgcac ccgctgcgct ggccacgctg acggaccgag
      721 agcgcgaggt cctcgacctg gtcgcggccg ggctgtcgaa ccgtgacatt gccgaccgcc
      781 tcttcatcag cgaacggact gctgccgtcc acgtgtcgaa cgtgctgggc aagctgggag
      841 tttcccgccg cacagaggca gcagccatcg cgttgagcgg ccgacctccg tcctgacccg
      901 ctcgcggtct gacgagcgag aaatagtcat tcctgacgat ccccccgcat ccggatgcgg
      961 tcgacacttg acgggtactc atcttcaggt tcctgcccgc cgtgcccacc ctggtccgcg
     1021 tctgggacgc atcccgagtt gtgctgccac tcgcgctcgc cgtgatcgcg tgcgtcgccc
     1081 tgccccctgt gccggcccgc gccgccaccc cgacgcctga cgccgtgcgt gagttcctgg
     1141 aggggtgggc atcggtctct ttgccttctt ccacatcctg atcaatcccg cgtctggcta
     1201 cctgtccgac tccagccgca cgccgctgtt caccattttg gcgttgctca tcggattcag
     1261 cgcgctatcg gtcgccttct gggcgtggtt ccggttccgg aacgcccccc tgaggtgccg
     1321 gttgacgtcg atctgtcccg accatcggtc cattgaggga ggtttccatg tttcgtccgg
     1381 ccattgccct gcttgccctg ctgctgcttg ccaccgcctg tggtggaggc accacgccca
     1441 gcagcgccgc ctcggcagcc gattcaaccc agagttcggc tgcgagccct gatacagagc
     1501 cgagcgaggt cgccgaggca tccgtcgagc ccagtgctgc ggaggcaacg gcctgcgacc
     1561 tggtgccggg cgaggtggcg ggatacaccc tgaacgctgc gggggaccgc gaggtgacca
     1621 cgccggactc cctggaggca gaggccgagg cgacagagga ccaggtgctg gccgatcagc
     1681 tccgtcagcg ggccaccttc tggaacacga tgggcatcat cgagagctgc cagcgctcgt
     1741 acgacgccga gggtgcgcgg atcatcgtgg ccgcgacgag attcatgaac gagcaggggc
     1801 ccgagcagta ctacgccagc gagtggtcgc aagggtgcca acctgtcgtc caggacttcc
     1861 cagctgcccc ggacgcccag ctctacgact gccccggcct tctcccggcg gcctacgcgg
     1921 tggccgtggt ggtggatgga accacgggtt gggagatcat ctcaaggctt gattatccga
     1981 ccggcgggac catgaccgac gagatgcgtg ccgagatgct ggctgatgcc ttcgttgccg
     2041 ctggcacggt ggccgaagtc atccaaggcg acgggtaggc ctgaactgtc cgtcttcacg
     2101 ccacatgacc aatacgtgcg g
//
LOCUS       DATACA010000298         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1724557, whole genome shotgun sequence.
ACCESSION   DATACA010000298 DATACA010000000
VERSION     DATACA010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724557"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..523
                     /locus_tag="VHK28_06100"
     CDS             35..523
                     /locus_tag="VHK28_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX2141835.1"
                     /translation="MADSSFDVVSDFDQQELVNALDQVRREVATRYDFKGAKVSLELT
                     KTEIILQADDEYRANAVKDLIQSKAVRRNLSLKIFDWGKLEPAGGGTVRQHIGLKRGL
                     TSEQAKEIAKIIREAFPKVKPTIQGDAVRVSGKSKDDLQGVIAHLRGMDYPVALQFEN
                     YR"
     gene            520..1038
                     /locus_tag="VHK28_06105"
     CDS             520..1038
                     /locus_tag="VHK28_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX2141836.1"
                     /translation="MIPTPTEAELEVAGARVGDALLERGWRMATAESSTGGLIGHAIT
                     MVPGASQYYVGGVIAYSNLAKEVELGVPQDMIEEHGAVSDPVARAMAEGVRRRFKVEM
                     GVSVTGVAGPEGGSRDKPVGTHYVAVSLRGQPDRVEHRVFPHDRDGNKAAAAMLALQL
                     AGEVLAATHDAE"
     gene            1048..2487
                     /gene="murC"
                     /locus_tag="VHK28_06110"
     CDS             1048..2487
                     /gene="murC"
                     /locus_tag="VHK28_06110"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX2141837.1"
                     /translation="MVRPGERIHLIGIAGSGAAGLALLAHHAGADIDGCDADTPSPYT
                     PPLEAAGIRVSSGHDPAHLGGVERVAVTPALRAAPNLPELQAAYEAGLPVVTWQAILG
                     ELMAAPGRIGLAVTGTHGKSTTTALLGHLLESAGMDPTVEVGAFIEAWGATVRPGGGA
                     PFLVEADEFGDNFLNYHPAGAIVTNVEMDHPDYFADREAVLASMERFVRGMGESEALG
                     GRLLLTTADDLGAQELLGRLSGWQGRVIRYGPGGEVEAVDVGFADGGASFRLFGRRFV
                     SPLAGTHNVLNATAALVLARELGADLEALAEGLRSFAGAGRRMELIADTAGVTVYDDY
                     GHHPTEVRATLQAMRQRVGDRRLWAVFEPHMYSRTALLFDEFTRAFTDADEVVIADIF
                     ASRDTDEALRATSAEALADGIERISAVPAIATGDADATATYVAEHLRRGDAVLVMGAG
                     KSYRIAQGLKAALEAGVLDAPRSISGAES"
     gene            2484..2861
                     /locus_tag="VHK28_06115"
     CDS             2484..2861
                     /locus_tag="VHK28_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141838.1"
                     /translation="MSGARVAVLGILLAVAVAVLDQGLVWLVEASGARDQPAVILVLL
                     LSAFLGLLTAVLVRAVDLPGALAPTTLVLAWIVVPPVLGAIPAAATEAFGRDAALSAT
                     DAVALAVAVVAATVTLAVSNAYD"
     gene            2869..3711
                     /locus_tag="VHK28_06120"
     CDS             2869..3711
                     /locus_tag="VHK28_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX2141839.1"
                     /translation="MLPGDSRPVRRTSSWIIVPGAVLVLTAIGVVLPLRDGPTAFLAG
                     TAPAIHAACLLLLPLGVARLDATLAGLVAAAVLAGFVAYPTAPARQLVQGDGSLRIMT
                     WNLHGEEVAGVGLPGALARWSPDVLVLQEAETDAVMDGALPPGMEALPFPEAGTPPGM
                     LLASNLPILDSGEIRMPATAWDRARAFWMRLDAAPHPVTVVAVHLAVPIHPSSLPCPY
                     CPTLRDAQVAAVAQFIRARMEAGEVVILAGDLNLNEREVAYHDLAFLTDAARGPTWRH
                     QERA"
BASE COUNT          539 a         1333 c         1387 g          555 t
ORIGIN      
        1 cgcacccgcc ccgacgcgcg aagatagggg agccatggcc gattcatcat tcgacgtcgt
       61 cagcgacttc gaccagcagg agctggtcaa cgccctcgac caggtccgcc gcgaggtggc
      121 cacccggtac gacttcaagg gagccaaggt gagcctggag ctcaccaaga ccgagatcat
      181 cctccaggcc gacgacgagt accgcgccaa cgcggtcaag gacctcatcc agtccaaggc
      241 ggtccgccgc aacctgtcgc tcaagatctt cgactggggc aagctggagc ccgccggagg
      301 cggcacggtc cggcagcaca tcggtctcaa acgtggcctg accagcgaac aggccaagga
      361 gatcgccaag atcattcgcg aggcgtttcc caaggtgaag cccaccatcc agggcgatgc
      421 ggttcgcgtc agcgggaaga gcaaggacga cctgcagggc gtcatcgcgc acctgcgtgg
      481 gatggactac cccgtggcgc tccagttcga gaactaccga tgatccccac gccgaccgag
      541 gcggagctgg aggtcgcggg tgcccgcgtg ggcgatgcgc tgctggaacg cgggtggcgg
      601 atggcgaccg ccgagtcgtc gaccggcggg ctcatcggtc atgcgatcac catggtgcct
      661 ggcgccagcc agtactacgt cggcggtgtg atcgcctact cgaacctggc caaggaggtc
      721 gagctcgggg ttccgcagga catgatcgag gagcacggtg ccgtctctga tccggtggcg
      781 cgcgccatgg ccgagggcgt ccgccgccgc ttcaaggtgg aaatgggggt atcggtcacc
      841 ggggtggccg gcccggaggg tggcagccgc gacaagccgg tggggaccca ctacgtcgcg
      901 gtctccctgc gcggccagcc ggaccgggtg gagcaccgcg tgttcccgca cgatcgcgac
      961 ggcaacaagg ctgcagccgc catgcttgcg ctccagctcg ccggcgaagt gcttgccgcc
     1021 acccacgacg ccgagtagcg ccgcgccgtg gtgcgcccgg gcgagcggat ccacctgatc
     1081 ggcatcgcgg ggtcgggtgc cgccgggctg gcgctcctgg cacaccacgc cggagccgac
     1141 atcgacggtt gtgacgccga tactccctcg ccctacaccc caccgttgga ggcggccggc
     1201 atccgggtca gcagcggtca cgatcccgcc cacctgggcg gggtggagcg cgtggccgtc
     1261 accccggccc tgcgggccgc cccgaacctg cccgagctgc aggcggcgta cgaggccggg
     1321 ctgccggtcg tgacctggca ggcgatcctg ggcgagctga tggcagcgcc gggacggatc
     1381 ggcctggcgg tgaccggcac acacggcaag agcacgacca ccgccctgct cggccacctg
     1441 ctggaatcgg ccgggatgga tccgaccgtc gaggtgggcg ccttcatcga ggcctggggg
     1501 gcgaccgtga ggcccggcgg gggagcgccc ttcctggtgg aagcggacga gttcggcgac
     1561 aacttcctga actaccaccc cgccggggcg atcgtgacca acgtggagat ggaccacccc
     1621 gactacttcg cggaccggga ggcggtgctg gcatccatgg agcgcttcgt gcgcgggatg
     1681 ggggagagcg aggccctcgg cggccggctg ctgctgacga ccgccgacga cctgggggcg
     1741 caggagctgc tgggccgtct gtccggctgg cagggccgcg tgatccgcta cggccccggc
     1801 ggcgaggtcg aggcggtcga cgtggggttc gcagatggag gcgcgagctt caggctgttc
     1861 gggcgccggt tcgtctcccc cctggccggg acgcacaacg tgttgaacgc cacggccgcc
     1921 ctggtcctgg cgcgggagct gggggcagac ctcgaggccc tggcggaggg actgcgcagc
     1981 ttcgccgggg cgggccgccg catggagcta atcgccgata ctgccggcgt gacggtctac
     2041 gacgactacg ggcaccaccc gaccgaggtc cgcgccactc tgcaggccat gcggcagcgg
     2101 gtcggggatc ggcggctctg ggcagtcttc gagccgcaca tgtactcccg gaccgccctg
     2161 ctcttcgacg agttcacccg cgctttcacc gatgccgacg aagtcgtcat cgccgacatc
     2221 ttcgcctcgc gcgataccga tgaggccctg cgcgccacct ccgccgaggc cctggccgat
     2281 ggcatcgagc gcatctctgc ggtcccggcc atcgccaccg gcgacgccga tgccaccgcc
     2341 acctacgtgg ccgagcacct gcgccgcggg gacgccgtgc tggtgatggg tgccggcaag
     2401 tcgtaccgca tcgcccaggg gctcaaggcc gcgctcgagg cgggcgtgct ggacgcgccg
     2461 cgttccatct cgggagcgga gagttgagcg gggcgcgcgt cgccgtcctg ggcatcctgc
     2521 tcgcggtcgc ggtcgccgtc ctggaccagg gcctggtgtg gctggtggag gcctccggcg
     2581 cacgggacca gccggccgtc atcctggtcc tgctgctgtc ggcgttcctg ggcctgctga
     2641 ccgcggtcct ggttcgcgcc gtggaccttc ccggcgcact ggcgcccacc accctggtgc
     2701 tggcctggat cgttgtgcca ccggtcctgg gcgccatccc ggcggccgcc accgaggcct
     2761 tcggccgcga cgcagccctg tcggcaaccg atgccgtggc cctcgccgtg gccgttgtgg
     2821 ccgccaccgt cacgctggcc gtctccaacg cctacgactg aggccagcgt gctgccgggg
     2881 gactcgcgac ccgtgcggcg gacgtccagc tggatcattg ttcctggcgc cgtcctggtg
     2941 ctgaccgcga tcggcgtggt cctgccgctc cgcgacggtc cgacggcatt cctggctgga
     3001 accgctcccg ccattcatgc agcctgcctg ctcctgctgc cgctgggggt cgccaggctg
     3061 gacgcgacgc tcgccggact ggtggcggcc gcggtactgg ccgggtttgt ggcctatccg
     3121 acggcgcccg cgcggcagtt ggtccagggc gacgggtcgc tccggatcat gacctggaac
     3181 ctccacggcg aggaggtggc gggggtggga ctgcccggtg cgttggcgcg atggtcaccc
     3241 gacgtgctcg tgctgcagga ggcggagacc gatgcggtga tggatggcgc gctgccgccc
     3301 gggatggagg ctcttccctt ccccgaggcc gggacgccgc cgggcatgct gctggccagc
     3361 aacctcccga tcctggacag tggggagatc cggatgccgg ccacggcgtg ggaccgtgcc
     3421 cgagccttct ggatgcggct ggacgctgca ccacaccccg tcacggtcgt cgcggtgcat
     3481 ctcgcggtcc cgatccaccc ctcgtcgtta ccgtgcccgt actgccccac gctgcgcgac
     3541 gcgcaagtgg cggccgtggc ccaattcatc cgcgctcgaa tggaggccgg cgaggtggtg
     3601 atcctcgccg gcgacctgaa cctgaacgag cgagaggtcg cctaccacga cctcgccttc
     3661 ctgaccgatg cggcccgcgg acccacctgg cggcaccaag agcgggcgtg aggaagacgt
     3721 agtccaggcg cagcacgggc ggcagccagt tgatcggcag cggccgccag gtgggtccgc
     3781 gggccgcatc ggtcaggaag gcgaggtcgc ggta
//
LOCUS       DATACA010000299         5826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1243116, whole genome shotgun sequence.
ACCESSION   DATACA010000299 DATACA010000000
VERSION     DATACA010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243116"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="VHK28_06125"
     CDS             <1..125
                     /locus_tag="VHK28_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141840.1"
                     /translation="RDDAAWLKHSFAFAAPDGGQTRLAYKPVTVTKFEPKPRTY"
     gene            131..835
                     /locus_tag="VHK28_06130"
     CDS             131..835
                     /locus_tag="VHK28_06130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX2141841.1"
                     /translation="MDIKLRIRRFNPEVDPEAHWEEYPLSAEPSDRVLDLLHQVKWYQ
                     DGTLTFRRSCGHGVCGSDAMLINGKNRLACEYLVKDAGSNISVEPIRGLPVVKDLLVD
                     MEPFWDQYRNVMPYLVNDEIPEDGKERRQSPEDRALYEDTSRCILCAACTTACPVFWT
                     DPDYVGPAAIVNAHRFISDTRDHAHEERLEIMADANGVWRCRTIFNCVEACPRDIKIT
                     RAIMEVAGEIQKHAGD"
     gene            828..1265
                     /locus_tag="VHK28_06135"
     CDS             828..1265
                     /locus_tag="VHK28_06135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX2141842.1"
                     /translation="MTDAAARSAVRRFVIHTDGAARGNPGPAGAGAVLRDASDGSLVA
                     ELAIFLGVRTNNYAEWTAVERALEEALARGASHVDLRTDSELVARQISGRYRVKHPDL
                     KPIHARVMALLARFAGYTVAHVPRELNKEADRLSNVAIDQVRQ"
     gene            1275..1730
                     /locus_tag="VHK28_06140"
     CDS             1275..1730
                     /locus_tag="VHK28_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141843.1"
                     /translation="MNRLRVPVAIGLLLVLAACDQAAETAGLRTPPPFPGSAAALEWS
                     DAGAVPPGFGPDDRGYASPQALIDAIVAFAQEERGPGGGVEMEAHLLGSDGRGGAVGV
                     IYVLGSFDDAVAGDEVQLSMLPGDDGQWRVAGARVRTHCRRGVSEGLCR"
     gene            1778..2100
                     /gene="rnpB"
                     /locus_tag="VHK28_06145"
     ncRNA           1778..2100
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VHK28_06145"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2126..2365)
                     /locus_tag="VHK28_06150"
     CDS             complement(2126..2365)
                     /locus_tag="VHK28_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141844.1"
                     /translation="MAIEKIQSGERLPVPRQGRIRRCRVRHVSIGPGHDGRPVAQVDC
                     LLGGQDHPLPLGSMDEARPICNACTATTVWRADED"
     gene            2566..3480
                     /locus_tag="VHK28_06155"
     CDS             2566..3480
                     /locus_tag="VHK28_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141845.1"
                     /translation="MFGRRKQKEAAEAAREAERRALFEELAKRPDTICPFLGLAEART
                     KYKDGVTDEHRCYAFGDPAELSHEQQQKVCLQRGYGNCPRYLRGVLVIPTEELEALRR
                     PQPLPPPSVPGEPEPAGGERGGRRPLAALLLVLLLAVGVGGAGAFFLLGDRDGFGSTT
                     PTPGPTATPLVTAASSEAPAPTTASQSAAPTATPAVTPPPEATPAPEDTFIGYEVIVP
                     AGQYTVYRVDDAGEIVDQLGTSFNDWSPGEVGRIVAENGLLHWRTLSGGLTGWSYIRG
                     QTATFFVREVFQAPDGSRSYELLPEEDL"
     gene            3836..4285
                     /gene="mraZ"
                     /locus_tag="VHK28_06160"
     CDS             3836..4285
                     /gene="mraZ"
                     /locus_tag="VHK28_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX2141846.1"
                     /translation="MDRGPFLTGEFRHALDDRGRVAIPARFRARLAGGATLARWLDRC
                     LGLFPPDEWEDLASKLGSLPLTNPRAREFARFMSSGAVDVELDRQGRVLVPGYLREYA
                     GINDGEVVVVGALSRLELWSPSAWQPYRNRIEDEPEALAEHLADLGI"
     gene            4382..5335
                     /gene="rsmH"
                     /locus_tag="VHK28_06165"
     CDS             4382..5335
                     /gene="rsmH"
                     /locus_tag="VHK28_06165"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX2141847.1"
                     /translation="MGEGHLPVMVDEVLASLDLRPGSSIADCTVGGGGHAERLLEAAS
                     PDGRLLGLDADRAAISEAKRVLARYGDRVVLRQANFAEIYGVAVEEGFVPMDGALFDL
                     GLSSYQLADPGRGFSFTAQGAPDMRFDEASGLSALELIDRSSERDLAGILATFGEERR
                     AGRIAKAILAARAEGRLMTATDLADVVARAAPARGADARRIHPATRTFQALRIAVNRE
                     LEVLGAGLAGALAALRPGGRMAVISYHSGEDRIVKRFMARESRDCVEEPLPPVCTCGH
                     LAQLRVLNKGGTTPGDAEVRRNRRARSARLRVAEKIAPAGA"
     gene            5404..5571
                     /locus_tag="VHK28_06170"
     CDS             5404..5571
                     /locus_tag="VHK28_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141848.1"
                     /translation="MYSARQREVRERRAREAHQEEYWLSEQVAAIEIEEGLDFESPTS
                     WTVRLPDRSAA"
BASE COUNT          858 a         2046 c         2067 g          855 t
ORIGIN      
        1 agcgcgacga tgccgcctgg ctcaagcact ccttcgcctt cgcggccccc gacggcgggc
       61 aaacccgcct ggcctacaag ccggtgaccg tcaccaagtt cgaaccgaag ccgcggacct
      121 actgatggcc atggacatca agctccgcat ccgccgcttc aacccggagg tcgacccgga
      181 ggcgcactgg gaggagtacc cgctgagcgc ggagccgtcc gatcgcgtgc tcgacctgct
      241 gcaccaggtg aagtggtacc aagacgggac cctcaccttc cgccgttcct gcggccacgg
      301 cgtgtgcggc tcggacgcca tgctgatcaa cggcaagaac cggctggcgt gcgagtacct
      361 ggtcaaggac gccgggagca acatcagcgt cgagcccata cgcggtctcc cggtggtcaa
      421 ggacctgctg gtcgacatgg agccgttctg ggaccagtac cgcaacgtga tgccgtacct
      481 ggtcaacgac gagatcccag aggacggcaa ggagcgccgc cagtctcccg aggaccgcgc
      541 cctgtacgag gacaccagcc gctgcatcct gtgcgcggcc tgcaccacgg cctgtcccgt
      601 cttctggacc gatccggact acgtcgggcc ggcggccatc gtcaacgcgc accgcttcat
      661 cagcgacacc cgcgaccacg cccacgagga gcggctggag atcatggccg atgccaacgg
      721 cgtgtggcgc tgccgaacca tcttcaactg cgtggaggcc tgcccacgcg acatcaagat
      781 cacccgagcc atcatggagg tcgccggcga gatccagaag cacgccggtg actgacgccg
      841 cggcccgctc cgccgtccgc cgcttcgtga tccataccga tggcgccgct cgcggcaacc
      901 ccggccccgc gggcgccggg gcggtcctgc gcgacgcgtc cgatggctcg ctggtcgccg
      961 aactggccat cttcctgggg gtgcgtacca acaactatgc ggagtggacg gccgtggagc
     1021 gcgccctcga ggaggcgctg gcgcgtggcg ccagccacgt ggacctgcgc accgattcgg
     1081 agctggtggc gcgccagatc tcggggcgct atcgcgtcaa gcatcctgac ctcaagccga
     1141 tccacgcccg ggtgatggcc ctgctggcgc gcttcgcggg gtacaccgtg gcgcacgtcc
     1201 ccagggagct gaacaaggag gcggatcggc tctcgaacgt ggccatcgac caggtccggc
     1261 agtaacgtac tgccgtgaac cggctccggg tgcctgtcgc catcggcttg ctgctggtgc
     1321 tggccgcctg cgaccaggct gccgagacgg ccggcctgcg cacgccgccc ccattccccg
     1381 gatcggccgc cgcgctcgag tggtcggatg cgggcgcagt cccgcccggg ttcggcccgg
     1441 atgatcgggg gtacgcgtcc ccacaggcgc tgatcgacgc catcgtggcc ttcgcccagg
     1501 aggagcgcgg gcccggcggc ggcgtggaga tggaggcgca cctcctggga tcggacggac
     1561 ggggcggagc ggtgggtgtc atctacgtgc tgggctcgtt cgacgacgcg gttgccggcg
     1621 acgaggtgca gctctcgatg ctccctggcg acgacggcca atggcgggtc gccggtgccc
     1681 gggtccgaac gcactgccgg cgcggggtca gcgagggcct ctgtcgctga gctccctatt
     1741 ggaacggctg ttcgaatgag gtacactatc ggtccctgga ggcggccgga cggtcgcggg
     1801 cgcatcggtc gaaaggccgc gtccgaggaa agtccgagct ccacagagca ggctcgcggg
     1861 taacgcccgt ccgccgtgag gcgaggacca gcgccacagt gacgatgccc gcgcaagcgg
     1921 gagtgaaacg cggcaagctc gagccggagc aagaccgaat aggagggcga aacccggcgt
     1981 tcgcgtcgga ggaggttgct cgctcagccc tcgggtaggt cgcacagatg gatgaccgtt
     2041 catgggaccg atgcgaatcg gtcccgggac agaactcggc ttacaggccg ctctccaggg
     2101 acagccttcg caccgtcggc cggtgctagt cctcgtccgc gcgccagacc gtggtggccg
     2161 tgcaggcgtt gcagatgggc cgcgcctcgt ccatggatcc caggggcagt gggtgatcct
     2221 gcccgcccag caggcagtcg acctgggcta ccggtcgtcc gtcatggccg ggaccgatgg
     2281 agacatggcg cacgcggcag cgacggatgc ggccctggcg ggggaccggc agccgctcgc
     2341 cgctctggat cttctcgatc gccacgtcgc ttcacctcga ctcgcaggcg tgctgcacac
     2401 ggctgcgcac gggtagggta ttgggccgta gctcgcgtcc tcaatgacgc cttcgtaccc
     2461 ccatggtccc cgttcggcgc ccgtggtccc acgctagact cagctcgctg ggccctgctt
     2521 gtgccggggc ggatggtctg aggcagggag gaactcgagc agctcgtgtt tggacgtcgc
     2581 aagcagaagg aggccgcgga ggccgcccgc gaggcggaac gccgtgccct gttcgaggag
     2641 cttgccaagc ggccggatac catctgcccc ttcctgggcc tcgccgaggc gcgcaccaag
     2701 tacaaggacg gcgtgaccga tgagcatcgc tgctatgcct tcggtgaccc cgccgagctc
     2761 tcgcacgagc agcagcagaa ggtctgcctg cagcgcggct acggcaactg cccgcgctac
     2821 ctgcgcggcg tgctggtcat ccccaccgag gagctcgagg cgctgcgccg gccgcagccg
     2881 ctgccgccgc caagcgttcc gggcgagccc gagcccgcgg gcggcgaacg cggcggacga
     2941 cgtccgctgg ccgccctgct cctggtcttg cttctggcgg tgggcgtcgg tggcgccggc
     3001 gccttcttcc tgctcggcga ccgcgacggg ttcggctcga ccacgccgac ccccggcccc
     3061 accgcaaccc cgctggtgac cgccgcctcg agcgaggcgc ctgcacccac caccgcgtca
     3121 cagtccgcgg ccccgaccgc gaccccggcg gtgacgccgc ctcccgaggc cacgccggct
     3181 ccggaagata cgttcatcgg ctacgaggtg atcgttccgg ctggccagta cacggtgtac
     3241 cgcgtggatg acgccggcga gatcgtcgac cagctcggaa cgagcttcaa tgactggagc
     3301 cccggcgagg ttgggcgcat cgtggccgag aacgggctgc tccactggcg caccctgtcc
     3361 ggcgggctca ccggttggtc ctacatccgg ggccagaccg ccaccttctt cgtgcgcgag
     3421 gtgttccagg ccccggacgg gtctcgttcc tacgaactgc tccccgagga ggacctctag
     3481 cctccacgtt cggcttgacc ggttcggcct gggcgccgcg cggtcacccg gtggctccaa
     3541 tggccaagca gccggcggat ttcgctgctg gatggccgcc ccacccaccg ggtgagcgcc
     3601 accgccatca aggcgtccgg cccggtcgct ccctggctgt tgtagccacc gcgtggccct
     3661 gacgcgacgt gattgggagc tgtggccctg acgcgacggg gccgatccat cggtcccatg
     3721 accaatggct gtgtgctgcc tgcacgaaat ggcttgacgg gtggtgcatg ccctgtagaa
     3781 tggcgccttg tggtccaaag tggggccaaa tggggattga tggtaggcat gcagcatgga
     3841 tcgtggcccc ttcctcaccg gcgagttccg gcacgccctg gacgatcgcg gccgggtcgc
     3901 gattccggcg cgcttccggg cgcggctggc ggggggcgcc accctggcgc gctggctgga
     3961 ccgctgcctg gggctctttc caccggacga gtgggaggac ctggcgtcga agctgggatc
     4021 cctgccgctg accaaccctc gggcgcgaga gttcgcgcgc ttcatgtcct ccggcgcggt
     4081 cgacgtggag ctggaccgcc aggggcgggt gctggtgccg ggctacctgc gcgagtacgc
     4141 cgggatcaac gacggggagg tcgtggtcgt gggtgcgctg agccggctgg aactgtggtc
     4201 tccatcggcc tggcagccct accgcaaccg catcgaggac gagccggagg cgctcgcgga
     4261 gcacctggcc gacctcggga tctgagcgag cgagacggac cgatgacgca cgtctcgacc
     4321 tcgccattga tgcgtctccg ggccgctcgg cccgccaccg accaacgagg tgcagaagcg
     4381 aatgggagaa gggcacctgc cggtcatggt cgacgaggtt ctcgcctctc tcgacttgcg
     4441 acccggcagc tccatcgcgg actgcacggt cgggggcgga gggcacgccg agcggctcct
     4501 ggaggccgcg tctccggatg gtcgcctgct cggcctggat gccgaccggg cggccatctc
     4561 ggaggcgaaa cgcgtgctgg cgcggtacgg cgaccgggtc gtgctgcgcc aggccaactt
     4621 cgccgagatc tacggggtgg ccgtggagga gggcttcgtg ccgatggacg gcgcgctctt
     4681 cgacctcggc ctgtccagct accagctggc cgatccgggt cgcggattca gcttcaccgc
     4741 ccagggcgct ccggacatgc ggttcgatga agcgtccggc ctctccgccc tcgagctcat
     4801 cgaccgctcg tcggagcgag acctcgccgg catcctcgcg acgtttggtg aggagcgccg
     4861 tgcaggccgc atcgcgaagg cgatcctggc ggcgcgtgcg gagggccggt tgatgaccgc
     4921 caccgacctg gcggacgtcg ttgcccgcgc ggcaccggcg cgtggggcgg acgcacgccg
     4981 catccatccc gcgacacgga cgttccaggc gctgcgcatc gccgtcaacc gagagctcga
     5041 ggtcctggga gctgggctcg ccggagccct ggccgcgctg cgccccggag ggcgcatggc
     5101 cgtcatcagc taccactcgg gcgaggaccg catcgtcaag cggttcatgg cccgtgaaag
     5161 tcgggactgc gtggaggagc cgttgccgcc ggtctgcacc tgcgggcatc tcgcccagct
     5221 gcgcgtcctg aacaaggggg gcaccacgcc aggcgacgcc gaagtgcgtc gcaatcggag
     5281 ggcacgcagc gcccggctgc gggtcgccga gaagatcgcg ccggccggcg cgtaagcagc
     5341 ggaaggaggg ttcgaaccgc catgagccga ggacgtccac gacaccaggc cagccgccga
     5401 cggatgtact ccgcgcgcca gcgcgaggtg cgcgagcgcc gcgcgcgcga ggcgcaccag
     5461 gaggagtact ggctctccga gcaggtggcc gccatcgaga tcgaggaggg gctcgacttc
     5521 gagtcgccca cctcctggac cgtccgcctc cccgaccgaa gcgcggcgta gggaggcagg
     5581 tacgtggccg tcatcggggc gcgcccggcg accgggatcg gcatcttcgg ccgacgttcc
     5641 cgcgtccgcc cgcgccccgc gacgcgtccg cgtccccgcg ccggagccgg ccgacgccgg
     5701 cgcgaggtga ccagcatctc cggcctgttg ctgtgcatcg cagcggcagc cgggctggcg
     5761 ctcttctacc tgagccagtc gtcccacgtg gccgccaccg gctaccgcat cgccgacctg
     5821 caggca
//
LOCUS       DATACA010000300         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2287804, whole genome shotgun sequence.
ACCESSION   DATACA010000300 DATACA010000000
VERSION     DATACA010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287804"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VHK28_06175"
     CDS             <1..1038
                     /locus_tag="VHK28_06175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2141849.1"
                     /translation="PVEVLDETDDEAAVRSLAAYLRREEIDLVHAHMFRAEVVGTRAA
                     VAAGTPVIVATVHSSRVRSPEDVAALAALTPAMDRLIVPSASIEAKVRAEGRGAARFA
                     VIPNGVDLARFALPVAACGLRRELGVPDGDVLVGVVARLEPEKGHEHLVAAWPAVMTA
                     APNAWLVMVGEGSQEAALRRQVAGLPRPVTERVLFAGRRADISAITAELDVAVLPSLR
                     EAQGISLLEAMARRVPVVASAVGGIPEVVTDGVDGLLVPPRSPSALADAIVRLARSAA
                     LRGRLGEAGYRTVAERFSIDAQVRQVEAVYDEELDRAGVTAALRASRTGSEGPSARRP
                     RGRAALEVPPV"
     gene            1674..1847
                     /locus_tag="VHK28_06180"
     CDS             1674..1847
                     /locus_tag="VHK28_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141850.1"
                     /translation="MDRWAGLAAIAGFAIWTVAFFAPASLAWLLTVANLVGGAGLAWM
                     GWSIWSRPMTVTA"
     gene            1902..3113
                     /locus_tag="VHK28_06185"
     CDS             1902..3113
                     /locus_tag="VHK28_06185"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2141851.1"
                     /translation="MAADGRLTAEELADEAGTSVERVAELAAAGVLQAGSEYTREDVP
                     RVLVANALEEGGLSLEVMRRGIEEGIVSFEQTPLVYPDPGSPGGTVAELAAELDLPTD
                     AVLRIITAFNLPRPAPEERLRQAHEDHLREFVAAWRPLGGEELLISAARIYGDAMRRA
                     AEGWMELFEEAVLAPVADRVLSWEEMSHYALDPGYRVLEVGHSLLPWLLDQHRVPALN
                     RLNFDSIERQLALIGVAPAVPREPPAIVFADLAGYTRLTEEHGDEVAATSAMRLAELA
                     DEVASAHDGRLVKLLGDGAMLHFARPVDAVSAALALRQAVAAAGLPPAHIGIHAGAVI
                     RRESDYFGRTVNVAARLSGQAGPGEVLLTPAVVDAVRSAGEELPPMEDAGALRLKGIP
                     EPVAALRIGPG"
BASE COUNT          441 a         1152 c         1271 g          497 t
ORIGIN      
        1 ccggtggagg tccttgacga gaccgatgac gaggcggccg tccgttccct ggctgcctac
       61 ctgcgccgcg aggagatcga cctggtccac gcccacatgt tccgcgccga ggtggtggga
      121 acccgggcgg cggtcgcggc cggcacgccg gtgatcgtgg ccaccgtgca ctcgtcgcgg
      181 gtgcgctcgc cggaggacgt ggccgcgctg gccgccctga cgccggccat ggaccggctg
      241 atcgtgccgt cggcctcaat cgaggccaaa gttcgggccg agggccgcgg cgcggcgcgg
      301 ttcgcggtga tccccaacgg agtcgacctg gcgcggttcg cgctgccggt tgctgcctgc
      361 gggttgcgcc gcgagctggg cgtgccggat ggtgacgtgc tggtgggcgt ggtggcgcgg
      421 ctcgagccgg agaagggcca cgagcacctg gtcgcggcct ggccggcagt gatgacggcc
      481 gccccgaacg cctggttggt gatggtgggc gaggggtcgc aggaagccgc gctgcgtcgc
      541 caggtggcag ggctgccgcg gccggtcacc gagcgcgtgc tcttcgctgg ccgccgcgcc
      601 gacatcagcg cgatcaccgc cgagctggat gtcgccgtcc tcccttccct gcgcgaggcc
      661 cagggcatct ccctgctgga ggccatggcc cgccgcgtgc cggtggtcgc ctccgcggtc
      721 ggcggcattc cagaggtggt gaccgatggc gtcgacggcc tcctggtccc cccgcggtca
      781 ccgtccgccc tagccgatgc gatcgtgcgg ctggcccggt ccgctgcgct gcgcgggcgc
      841 ctgggcgagg cggggtaccg gaccgtggcg gagcggttca gcatcgacgc gcaggtgcgg
      901 caggtggaag ccgtctacga cgaggagctg gaccgcgccg gcgtcaccgc cgcgctccgg
      961 gcgtctcgca ctgggtcgga ggggccgtct gcgcgtcgtc cacggggccg cgccgccctc
     1021 gaggtccccc cggtctaggt tgccgtggcg ttgccgccgc tacattgggc agttcttcac
     1081 gtgggccggg tgtggggtgt cagaacgtcg attcatcggg cagctcgcag cacgaaccga
     1141 caagttcctc gcccttcacg ctcacggctg cctgacataa cggcagtaca gcacgccgcg
     1201 gcgtcctctg gcgaattcct gccccataca ccggcgcgta gctcccgccc gcgcctcatc
     1261 ggcaaggcag gaactccacg atggcggcgc cggcgcggaa tccgggaggg tttcgtgcgg
     1321 aaatgcagta cgggtgcggc ataagagcca cgaccaggcg tcacgagccg gacatcgtca
     1381 gcgttgggcc gtaccggtac gacattgccc aaggaaacgg ccgcgcgcaa acgccacgag
     1441 cgaggttcgt gaacgttctg cctcacgggt caggcgcccg tcggcccagc acgcccaaac
     1501 gggcaccaaa cgaggctcgg tcacttgcct ggcagtcgca gaatagcgcc gtccgcgggc
     1561 atgacggcat ccgggttgcg gccggcaccg agcatcggcc ggaccgagag gactcccgcg
     1621 gaaacggagg tgtgcggtgc ctgctggggg tggccaccag ccgtgctccc cacatggacc
     1681 gatgggccgg cctcgccgcc atcgcgggct tcgcgatctg gacggtcgcc ttcttcgcgc
     1741 cggcgagtct ggcctggctg ttgacggtcg ccaacctggt cggcggcgcc ggcctggcct
     1801 ggatggggtg gtccatctgg tccagaccga tgacggtcac cgcctgatgc cgatcctgct
     1861 tgccctggcc ggggtggtgg cggcggcggt cgaggcggtg cgtggcggct gacggccggc
     1921 tcaccgcgga ggagctcgcg gacgaggcgg gcacgtccgt cgagcgggtc gccgagctcg
     1981 cggcagcggg ggtgctgcaa gcgggatcgg agtacacccg cgaggacgtg ccgcgcgtgc
     2041 tggtggccaa cgcgctcgag gagggcgggc tgtccctgga ggtgatgcgc cgagggatcg
     2101 aagagggcat cgtcagcttc gagcagaccc cgctggtcta ccccgatccc gggtcgccgg
     2161 gtgggacggt tgcggagctg gcagccgagc tcgacctgcc gaccgatgcg gtgctgcgca
     2221 tcatcaccgc cttcaacctc ccgcgcccgg ctcccgagga gcgcctgcgc caggcccacg
     2281 aggaccacct gcgcgagttt gtcgccgcct ggcgcccgct cggtggcgag gagctgctga
     2341 tcagcgccgc ccgcatctac ggcgatgcga tgcgtcgcgc cgccgagggc tggatggagc
     2401 tcttcgagga ggcggtcctg gctcccgtcg ccgaccgcgt cctgagctgg gaggagatga
     2461 gccactacgc gctggatccc ggctaccggg tgctggaggt gggccactcg ctgctgccgt
     2521 ggctgctgga ccagcaccgc gtcccggcgc tcaaccggct gaacttcgat tccatcgagc
     2581 ggcagctggc gctgatcggc gtggcgccgg ccgtgccccg cgagccgccg gccatcgtct
     2641 tcgcggacct ggccgggtac acccggctga ccgaggagca cggtgacgag gttgccgcca
     2701 cctcggccat gcgcctcgcc gagctggccg acgaggttgc ctccgcccac gacggccgcc
     2761 tggtcaagct gctgggcgac ggcgccatgc tccacttcgc gcgtccggtt gatgcggtat
     2821 ccgccgcgct cgccctgcgc caggcggtgg cggccgccgg cctgccgccg gcgcacatcg
     2881 gtatccatgc cggcgcggtg atccgccggg agtcggacta cttcggacgg acggtgaacg
     2941 ttgccgcgcg gctatcgggc caggccgggc cgggagaggt gctgctcacg cccgcggtcg
     3001 tggacgccgt gcggtcggcc ggggaggagc tgccgccgat ggaggacgcg ggcgcgttgc
     3061 ggctgaaggg aataccggag ccggtggccg cgctgcgcat cggtcccggc tgacggggcg
     3121 gtccgtgcat caccggcacg gcaggaactc cacgatcgtg gcgccggcgc ggaatccggg
     3181 agagtttcgt gcggaaatgc agtacgggtg aggcataaga gccacgaccc ggcgtcacga
     3241 gcgtgacatc gtcagcgtta agctgtaccg gttcgacatt tcgcaacgaa acgtcccccg
     3301 caggaggcca cgagcgagat tcgtggacgt tcggcctcac gggtgaggca ccggcctcca
     3361 c
//
LOCUS       DATACA010000301         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_922445, whole genome shotgun sequence.
ACCESSION   DATACA010000301 DATACA010000000
VERSION     DATACA010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922445"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2175)
                     /locus_tag="VHK28_06190"
     CDS             complement(<1..>2175)
                     /locus_tag="VHK28_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit A"
                     /protein_id="HEX2141852.1"
                     /translation="TGSNQSVLSGGPNAPPSVNIPWIPELKLELAFRMDTLSAVLAIL
                     ILGVGALVLFYCARYFKADDPNIGPFGAQLLAFAGAMFGLVITDDLLMLYVFWELTTV
                     LSYLLIGYARHRLAARRSALQALIVTTFGGLTMLVGLIMVGQTAGTYRMSEIMTAAPE
                     LVQHGMLIDVAILLILVGAISKSALVPFHFWLPAAMAAPTPVSAYLHAAAMVKAGIYL
                     VARFAPGFSDTRYWEGTVMVLGLATMLVGGWLALKQFDLKLILAYGTVSQLGFLTMVV
                     GLGSRDGALAGLGLLLAHGFFKATLFLVVGIIDHQSGTRDIRRLSGIYRAAPVLFTVS
                     LIGAASMAGVPPLLGFVAKESVYETFVHQASEGGWTGPVLLAGVVVGSMLTFAYSARF
                     VWGGFAYKPNVKKTPFKRIPGAFIAAPMLLTAATVVYGLWPSPLDSIIAPYVSLFPSD
                     GEPDGHLALWHGLTPALGLTVLTLAVGTLLFLFRAGVTAFQDRLSGGLTGERAYRGII
                     GVLDEVAVWVTGRTQRGSLVFYLVVILSVSVVTPLSAVFLMDAPWPDSWIAFDSPAQL
                     LAALGIIVAAILAIRANKRFMAVLMVAVAGYGIALIFALQGAPDLALTQMLVETIVLV
                     AIVLALRSLPARLWLRTPSSFKWLRALIGVAFGLAMVVIAMTSMAARTQLPVSLEYPQ
                     LAYDGGGGANVVNVTLVDIRAWDTYGELSVLAVAATGVASLIF"
BASE COUNT          467 a          689 c          724 g          295 t
ORIGIN      
        1 gaagatcagg ctggccacgc ccgtggcggc gaccgccagt acagagagtt cgccgtaggt
       61 gtcccacgcg cggatatcca ccaaggtaac gttgacaacg ttggctccgc cgccgccgtc
      121 gtaagccagc tgcgggtatt ccaacgagac cggcagctgg gtgcgcgcag ccatggacgt
      181 catggcaata accaccatgg ccaggccgaa cgccaccccg atcagggccc gcagccattt
      241 gaaactgctg ggtgtgcgca gccagaggcg tgccgggagt gagcgcagtg ccagcacaat
      301 cgcgaccagc acaatggtct ccacgagcat ctgggtcaag gccaggtccg gtgcgccctg
      361 cagtgcaaag atcagcgcga tgccgtaacc ggcgacagcg accatcagca cggccatgaa
      421 ccgcttgttg gccctgatgg cgaggatggc cgccacgatg atgcccagtg ccgcgagtaa
      481 ttgcgcggga gagtcgaaag cgatccagct gtcgggccag ggtgcgtcca tcaggaacac
      541 agcgctcagc ggagtgacaa ccgagacgct gaggatgacc acgaggtaga aaaccagcga
      601 accgcgctgg gtgcggccgg taacccagac ggccacctca tcgagcacgc cgatgatccc
      661 gcggtaggcg cgttcgccgg tcagcccacc ggagagacgg tcctggaacg cggtgacccc
      721 ggcccggaac aggaacagca gcgtgcccac agccagcgtc agcactgtca gtccgagcgc
      781 cggggtgagg ccgtgccaca gagccaggtg cccgtcgggc tcgccgtccg agggaaacag
      841 cgaaacgtag ggagcgatga tcgaatccag cggggaaggc cagagaccat acaccacggt
      901 ggccgccgtc agcagcatcg gcgctgcgat aaaagctccg ggaatgcgct tgaacggcgt
      961 cttcttgacg ttgggcttgt aggcgaaccc gccccagaca aaccgcgcgc tgtaggcgaa
     1021 ggtcagcatg gacccgacga cgacgccggc cagcagcaca gggccggtcc agccgccttc
     1081 ggaagcctgg tggacaaagg tctcgtacac tgactccttg gccacgaagc ccagcagcgg
     1141 cggtacccct gccatggagg cggcaccgat cagcgacacg gtaaaaagga ccggcgccgc
     1201 gcggtaaatt cccgaaagcc ggcggatatc gcgggtgccg gactgatggt cgatgatgcc
     1261 gacgaccagg aagagcgtcg ccttgaagaa gccgtgtgcc agcagcagtc ccaggcctgc
     1321 cagcgcgccg tcacggctgc ccagccccac caccatggtg aggaagccca gctggctgac
     1381 cgtgccgtag gccaggatga gcttcaggtc gaactgcttc agggccagcc acccgccgac
     1441 cagcatggtc gccaggccca ggaccatcac ggtcccctcc cagtaccggg tatccgagaa
     1501 gcccggcgcg aaccgtgcca ccaggtagat gccggccttg accatggctg cggcatggag
     1561 ataggcgctg accggtgtgg gtgcggccat cgcggcaggc agccagaagt ggaacgggac
     1621 cagggccgac ttgctgatgg cacccaccag gatcagcagg atggcgacgt cgatcaacat
     1681 gccgtgctgg accagttccg gggctgcggt catgatttcg gacatgcggt aagtacccgc
     1741 cgtctgcccg accatgatca ggccgacgag catggtcagg cccccgaagg ttgtcacgat
     1801 cagggcctgc agggccgaac gccgcgccgc cagcctgtgc cgcgcgtagc cgatcagcag
     1861 gtaggacagg accgtggtca gttcccagaa gacatagagc atgagcaggt catcggtgat
     1921 gaccaagccg aacatggctc cggcgaaggc cagcagctgc gcaccgaacg gcccgatatt
     1981 gggatcatcg gccttgaagt agcgcgcgca gtaaaagagg accagggcgc cgacaccgag
     2041 aataaggatc gcaagtacgg cggacaaggt gtccatccgg aaggccagct caagcttcag
     2101 ctccggaatc cacgggatgt ttaccgacgg cggtgcattg ggtccgccgg agagtacact
     2161 ttggttcgat cccgt
//
LOCUS       DATACA010000302         5418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2288140, whole genome shotgun sequence.
ACCESSION   DATACA010000302 DATACA010000000
VERSION     DATACA010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288140"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1122
                     /locus_tag="VHK28_06195"
     CDS             46..1122
                     /locus_tag="VHK28_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141853.1"
                     /translation="MLQFVVRRLLLSLPVLFGIIFLVFALTRLLPGDPCRAALGERAT
                     DAICDAFNARFGLNEPIPVQFVNYLGNLASGDMGNSIRQGRPVAEILVERLPVTLELT
                     LFALMFAVVVGVTLGRLAAMRRNSPIDVATMFLANLGVSIPVFVLGLVLAYLFAVVLR
                     GTPFALPPSGQLSPGIGFQPLAVQWGMQEVEGFPRAVLDFVSGMRVTASLISGQWETF
                     VDGLKHLILPMVALGTIPMAVIARMTRSSMLDVLGLDYVRTARAKGAPERIVRTRHAF
                     RNALLPVVTVAGLSLGSLLSGAVLTESIFLLPGVGRTLFEAITGRDYIVVQAFTLIIA
                     VLYVVINLIVDVSYAYLDPRIRLR"
     gene            1126..2091
                     /locus_tag="VHK28_06200"
     CDS             1126..2091
                     /locus_tag="VHK28_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141854.1"
                     /translation="MAATDLTTRVDDVATVARRPASLLRDTIGAILRQRSAVVGLSLL
                     GILFLAALLADVLAPYDYRETLFGVEGAQRLAGPCVHLFGCPTDQPQFLMGIDQNNRD
                     VFTRVLFGARVSLQVGLVTVGIAIVTGTLIGSIAAYLGGWVDNILMRFMDVLLAFPAL
                     ILAIFLVTTFDSLDLPLFQDRLFNAMLAIGIVAIPVYARVIRSSVISVRERDFVTASR
                     ALGESGAGILFRRILPNALTPLIVQGTLGIATAILEVAALSFLGIGAQEPTAEWGAMI
                     GQVRNLMFQAPHLVIFPGTALALSVLGFNLLGDGIRDALDPRLHR"
     gene            2105..3151
                     /locus_tag="VHK28_06205"
     CDS             2105..3151
                     /locus_tag="VHK28_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141855.1"
                     /translation="MTVTELRTSRSGPAEPGQRLLDVRGPQTSFHTRDGIVRAVTGLD
                     FHVNRGEVMWLVGESGCGKSVSSLSILRLIAPPGRIEAGEIMFDGVDLLNLREREMRQ
                     LRGERISMVFQQPTSSLNPVYDVGMQVGEVLELHRSMKRKAARDRAMELLRMVGIPDP
                     RRRLDAFPHELSGGMAQRVMIAMALACEPELLIADEPTTALDVTIQAQILDIMRSLQK
                     ETGTAIVLITHDLGVVAEMCDRVAVMYAGEIVEQSDVATLFADPRHPYTRGLIGSVPR
                     LGEDREELSVIPGSVPNLVDLPEACRFHPRCTARVELEVPYTDEIHPELRPVEEGHDV
                     RCWIYHERDGSLRS"
     gene            3157..4380
                     /locus_tag="VHK28_06210"
     CDS             3157..4380
                     /locus_tag="VHK28_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2141856.1"
                     /translation="MPAATAAPQPAADDRPLVEVERLRQYFPIHGGILQRKLGEVKAV
                     DDVTFTIRRGETLGLVGESGCGKTTVGRTLLRLIEPTEGRVVFDGVDLTALSRRELKA
                     MRRRMQIIFQDPFASLDPRTPIGASIGEGLRVHEIGTVAERRERVAAMMEKVGLQPYH
                     ARRYPHEFSGGQRQRIGIARALVLEPDLVVCDEPVSALDVSIQAQVLNQLRQLQRDLG
                     LTYLFVAHNMGVVEHISDRVAVLYLGTIAELAESKQIYRRPLHPYTIALLSAVPISDP
                     AQRGRQKRIILTGDVPSPVNPPSGCPFHTRCWLRRQLGNPENCATDRPALRTLTELGG
                     AADAEQQVACHYAEELLEPARREQLISRAAAGSSARLVTEDEEAGPTTLPPPDTGGLF
                     DRGTDASGEESHGRL"
     gene            4377..5264
                     /locus_tag="VHK28_06215"
     CDS             4377..5264
                     /locus_tag="VHK28_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX2141857.1"
                     /translation="MSELRIALAQLSPRLGEVDANCELAAEWVVRAAAAGAQLTVFPE
                     LALTGYLLSDLVPEVSIRRDDRRLAQLSRGAPGMLVAVGFVEETDDHRYANSVALLHD
                     GSVVGLHRKVYLPTYGMFDEGRFTRPGDRIRTVEVGEPLGRIGLSVCEDFWHPSLPML
                     QAQDGATLLVNVAAGPARAPGSAAGLRAIAGWHKMQETYALLGTVGIAFCNRVGNEEG
                     LTFWGGSRILAPDGAVVAEAPLYEEALVVGSLETDDLRMQRYGLPLLADERLELVRRE
                     VDRIVAERAGLHASEETSK"
BASE COUNT          729 a         1930 c         1871 g          888 t
ORIGIN      
        1 cccccccctt cccattcacc ctcagcccgc taccatgcgc gcctgatgct gcagttcgtc
       61 gtccgtcgcc tgctgctgag cctgccggtc ctcttcggca tcatcttcct cgtctttgcc
      121 cttacccggc tgctccccgg cgatccgtgc cgagcggcac tcggagagcg cgcgactgat
      181 gccatctgcg acgccttcaa cgctcgcttc ggcctgaatg agcccatccc cgtccagttc
      241 gtcaactacc tgggcaacct ggccagcggt gacatgggca actccatccg acagggccgc
      301 ccggtggccg agatcctggt cgagcgattg cccgtcacgc tcgagctcac cctcttcgcc
      361 ctcatgttcg cggtggtggt gggcgtgacc ctcggccgcc tggccgccat gcgccgcaac
      421 tcgccgatcg acgtggccac catgttcctg gccaacctgg gtgtctcgat cccggtcttc
      481 gtgctgggcc tggtgctggc ctacctcttc gccgtggtgc tgcgcggtac gccgttcgcg
      541 ctgccgccgt ccggacagct gtcgccgggg atcggcttcc agccactggc cgtgcagtgg
      601 ggcatgcagg aggtggaggg cttcccgcgc gccgtgctcg atttcgtgtc cggcatgcgg
      661 gtcaccgcct cgctgatatc gggccagtgg gagaccttcg ttgacggcct gaagcacctg
      721 atcctgccca tggtcgcgct gggcaccatt cctatggcgg tcattgcgcg catgacgcgc
      781 tccagcatgc tcgacgtgct cggcctcgac tacgtgcgaa cggcacgcgc caagggggcg
      841 ccggagcgca tcgtgcgcac ccgccacgcg ttccgcaacg cgctgctgcc ggtggtcacc
      901 gtggctggcc tgtcgctggg cagcctgctc tcgggcgcgg tcctgaccga gtcgatcttc
      961 ctgctgccgg gcgtgggccg cacgctgttc gaggccatca ccgggcgcga ctacatcgtg
     1021 gtgcaggcct tcactctgat catcgcggtg ctgtacgtgg tcatcaacct gatcgtggac
     1081 gtgtcctacg cctacctcga cccgcggatc aggcttcgct gagccgtggc cgccaccgac
     1141 ctcacgaccc gggtggacga cgtcgccacc gtcgcgcggc gccccgcctc gctgttgcgc
     1201 gacaccatcg gcgccatcct gcgccagcgc tcggccgtcg tcgggctcag cctgctgggc
     1261 atcctgttcc tggccgccct gctggccgac gtgctggccc cgtacgacta ccgcgagacg
     1321 ctcttcggcg tggagggcgc ccagcgcctg gcaggcccct gcgtgcacct cttcggctgc
     1381 ccgaccgatc agccgcagtt cctgatgggc atcgaccaga acaaccggga cgtcttcacc
     1441 cgcgtcctgt tcggggcacg cgtctcgctg caggtgggcc tggtgacggt cggcatcgcc
     1501 atcgtcaccg gcacgctgat cggctcgatc gctgcctacc tcggtggctg ggtcgacaac
     1561 atcctgatgc gcttcatgga cgtgctgctg gccttcccgg ccctgatcct ggccatcttc
     1621 ctggtcacca ccttcgacag cctggacctg ccgctcttcc aggaccgcct gttcaacgcc
     1681 atgctggcca tcggcatcgt ggccatccct gtctacgcgc gcgtcatccg ctcatcggtc
     1741 atttccgtgc gcgagcgaga cttcgtgacc gcctcgcgcg ccctggggga gtcgggggcc
     1801 ggcatcctgt tccgccgcat cctgcccaac gccctcaccc cgctgatcgt gcaggggacg
     1861 ctcggtatcg cgaccgccat cctggaggtc gccgcgctct cgttcctggg aatcggcgcc
     1921 caggagccga cagctgagtg gggtgccatg atcggccagg tgcggaacct catgttccag
     1981 gcgccccacc tggtgatctt ccccgggacg gcgctggcgc tctcggtcct gggcttcaac
     2041 ctgctgggcg acggcattcg cgacgccctc gacccgaggc tgcatcgttg acaatggtgg
     2101 accgatgacc gtcaccgagc tgcgaacctc ccggagcggc cccgccgagc ccggacagcg
     2161 cctgctcgac gtgcgcggcc cgcagactag cttccacacc cgcgacggga tcgtgcgggc
     2221 ggtgaccggc ctggacttcc acgtgaaccg cggcgaggtg atgtggctgg tcggcgagtc
     2281 aggctgcggc aagagcgtca gcagcctctc gatcctgcgc ctgatcgcgc cgccggggcg
     2341 catcgaggcc ggagagatca tgttcgacgg cgtggacctg ctcaacctgc gcgagcgcga
     2401 gatgcgccag ctgcgcggcg agcggatcag catggtcttc cagcagccga ccagcagcct
     2461 gaacccggtc tacgacgttg gcatgcaggt gggcgaggtg ctggagctcc atcggtccat
     2521 gaagcgcaag gcggcccgtg atcgcgccat ggagctgctg cgcatggtgg gcatcccgga
     2581 tccgcggcgg cgcctggacg ccttcccgca cgagctttcg ggtggcatgg cccagcgggt
     2641 catgatcgcc atggcgctcg cctgcgagcc ggagctgctg atcgcggacg agcctaccac
     2701 cgcactggac gtcaccatcc aggcccagat cctggacatc atgcgctccc tccagaagga
     2761 gaccggcacg gccatcgtgc tcatcaccca cgatttgggg gtagtggccg agatgtgcga
     2821 tcgggtggct gtcatgtacg ccggcgagat cgtggagcag agtgacgtgg ccaccctgtt
     2881 cgccgatccc aggcacccgt acacccgcgg gctgatcggc tcggtgccgc ggctgggcga
     2941 ggatcgcgag gagctgtcgg tcatcccggg cagcgtgccg aacctggtgg acctgcccga
     3001 ggcctgccgc ttccacccgc gctgcaccgc gcgcgtggag ctcgaggtgc cgtacaccga
     3061 tgagatccat cccgagctgc gcccggtcga ggagggccac gacgtgcggt gctggatcta
     3121 tcacgagcgg gacgggagcc tgcgatcgtg agcgccatgc cggcggccac agccgccccg
     3181 cagccggccg ctgatgaccg gcccttggtg gaggtcgagc gcctgcgaca gtacttcccc
     3241 atccacggcg gcatcctgca gcgcaagctg ggcgaggtga aggccgtcga cgacgtgacc
     3301 ttcaccatcc ggcgcggtga gacgctcggc ctggtcgggg agtccggctg cggcaagacg
     3361 acggtgggac gcacgctgct gcgcctgatc gagccgaccg agggcagggt cgtcttcgac
     3421 ggcgtggatc tcacagcgtt gtcccgacgc gagctcaagg ccatgcgccg ccggatgcag
     3481 atcatcttcc aggacccgtt cgcctcgctc gacccgcgga cgcccatcgg cgccagcatc
     3541 ggggagggcc tgcgcgtcca cgagatcggg acggtggccg agcgccgcga gcgggtggcg
     3601 gccatgatgg agaaggtcgg cctgcagccg taccacgccc ggcgctaccc gcatgagttc
     3661 agcggtggcc agcggcagcg catcggcatc gcccgtgcgc tggtgctgga gccggacctg
     3721 gtggtctgcg acgagccggt cagcgcgctg gacgtcagca tccaggccca ggtcctcaac
     3781 cagctgcgcc agctgcagcg tgacctgggc ctcacctacc tgttcgtggc gcacaacatg
     3841 ggcgtggtcg agcacatcag cgaccgggtg gcggtcttgt acctgggcac catcgccgag
     3901 ctggccgagt cgaagcagat ctaccgccgc ccactgcacc cgtacaccat cgcgctgctg
     3961 tcggcggtgc ccatcagcga cccggcgcag cgtggccggc agaagcggat catcctgacc
     4021 ggtgacgtgc cgtcgccggt caacccgccg tcgggctgcc cgttccacac ccgctgctgg
     4081 ctgcgccgcc agctgggcaa ccccgagaac tgcgcgaccg accggccggc gctgcgcacc
     4141 ctcaccgagc tgggcggggc ggccgatgcc gagcagcagg tggcctgcca ctatgcggag
     4201 gagctgctgg agccggctcg ccgcgagcag ctcatcagcc gggcggcggc cggcagctcg
     4261 gcgcggctgg tcaccgagga cgaggaggcc ggtcccacca cgcttccgcc gccggacacc
     4321 ggggggctct tcgaccgcgg cacggacgcc tccggcgagg agtcgcacgg ccgcctgtga
     4381 gcgagctccg gatcgcgctg gcccagctgt cgccgcgcct cggagaggtg gatgccaact
     4441 gcgagctggc cgccgaatgg gtggtacgtg cggccgccgc cggcgcacag ctcaccgtct
     4501 ttcccgagct ggcgctgacc gggtacctgc tctccgacct ggtgcccgag gtgtccatcc
     4561 ggcgcgacga ccggcggctg gcccagctct cacggggtgc cccggggatg ctggtggcgg
     4621 tcggcttcgt ggaggagacc gatgatcatc ggtacgccaa cagcgtggcc ctcctccacg
     4681 acgggtccgt ggtggggctg caccgcaagg tgtacctgcc gacctacggc atgttcgacg
     4741 agggccgttt cactcggccc ggtgaccgga tccggaccgt ggaggtgggg gagccgctcg
     4801 gacgcatcgg tctttcggtc tgcgaggact tctggcatcc ctcgctgccc atgctgcagg
     4861 cgcaggacgg cgccacgctg ctggtgaacg tggcggccgg tccggcgcgc gccccggggt
     4921 cggcagccgg gctgcgggcc atcgccggct ggcacaagat gcaggagacc tacgcgttgc
     4981 tgggcacggt ggggatcgcc ttctgcaacc gggtcggcaa cgaggagggg ctcaccttct
     5041 ggggcggctc tcggatcctg gccccggacg gcgccgtggt ggccgaggcg ccgctctacg
     5101 aggaggccct ggtggtcggc agcctggaga ccgatgacct gcgcatgcag cgctacggcc
     5161 tgccgctgct ggccgacgag cgcctcgagc tggttcggcg cgaggtggat cgcatcgtcg
     5221 ccgagcgcgc cggcctccac gcatcggaag agacttcgaa gtgagcgcgc cggagggcga
     5281 gcgcgcgcca ctgccggcca tcgagccgga gcaggcggtg caggtcatcg tcggcttcat
     5341 ccgctcgcag ctggagcagg ccgggttcgg gcgcctggtg gtcggcctgt cgggcggggt
     5401 cgactcggcc acggtcgc
//
LOCUS       DATACA010000303         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1404522, whole genome shotgun sequence.
ACCESSION   DATACA010000303 DATACA010000000
VERSION     DATACA010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404522"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VHK28_06220"
     CDS             complement(<1..778)
                     /locus_tag="VHK28_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016871827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2141858.1"
                     /translation="MSIETTPSAAAARQSRPRIQTELPGPRAREIIARDEAVVSPSLT
                     RAYPLVAESGNGCWVTDVDGNVFLDFAAGIAVASTGHAHPAVVSAIQEQAARLIHIAA
                     TDFYEPRYLEFSERLAAIAPFDEKARVFLTNSGTEAIEGAIKLARYHTRRPAIIAFEG
                     AFHGRTMGALSLTNSKVKQRAGFGPLLPMVYHAPFPRVRSWREGSGGDGSAELEVLRS
                     SIFGRLVAPTDVAAIVVEPVQGEGGYFPAPRTFMEGLRALC"
     gene            complement(829..1935)
                     /locus_tag="VHK28_06225"
     CDS             complement(829..1935)
                     /locus_tag="VHK28_06225"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX2141859.1"
                     /translation="MSGWRSLLYAAARPLLFRFEPERIHRLTLDALRMAGANRFGRPV
                     LTMAGGAPRRARPWALVAGLRFRNRIGVGAGFDKDAVALRGWAALGFGFAEVGTITPL
                     AQPGNPRPRLFRLTEDEALVNRMGFNNAGAAALARRVMLSRRHLPPDFVVGVNLGRGR
                     ATPREHAVDDYLAAHRLVAPVADYLVVNVSSPNTPGLRDLQDAAALRDLVGALSLAGR
                     RLRQERPILVKLSPDLGDEEFARLLAAAVDAGAVGIVVSNTSVARHALRSPHAGQAGG
                     LSGAPLMSRLLDRLRLARSLAGPELALAASGGIASAQDVADAYEAGADLVQLWTGLVY
                     RGPGLVGEAVRVLPTGGGGPSGSRGGAAATMVGV"
     gene            complement(1932..>2360)
                     /locus_tag="VHK28_06230"
     CDS             complement(1932..>2360)
                     /locus_tag="VHK28_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4288 domain-containing protein"
                     /protein_id="HEX2141860.1"
                     /translation="SLGELDELGRLDALDELEPVVDPSGWFGVQLRYAYLVGESQSVV
                     MIEDRVILVDGDDEEAALGRSLEIAEEYEDEFETEEGEQGQIRFEGVVALKELDDPPA
                     AGTEVWHEFMAPEGVETDAGGEPLPPVRPTEMAFPRAHDE"
BASE COUNT          348 a          875 c          828 g          309 t
ORIGIN      
        1 cgcacagcgc ccgcaggccc tccatgaagg tccgcggcgc cgggaagtag ccaccctcgc
       61 cctgcactgg ctcgaccacg atggcggcca cgtcggttgg cgccaccagg cgtccgaaga
      121 tcgaggagcg cagcacctcc agctccgcgc tcccgtcgcc acccgagccc tcgcgccagc
      181 tgcggacccg cgggaacggc gcgtggtaga ccatgggcag caacggaccg aagccggccc
      241 gctgcttgac cttcgagttg gtgagggaca gcgcccccat ggttcggccg tggaaggcgc
      301 cctcgaaggc gatgatggcc ggccgtctgg tgtggtaacg ggcgagcttg atggcgccct
      361 cgatcgcctc cgtgccggag ttggtgagga agacgcgcgc cttctcgtcg aacggggcga
      421 tggccgccag gcgctcgctg aactccaggt agcgaggctc gtagaagtcg gtggccgcga
      481 tgtgtatgag gcgagccgcc tgctcctgga tggcggagac gacagccgga tgggcgtgcc
      541 cggtgctggc caccgcgatg ccggccgcga agtccaggaa gacgttcccg tcgacatcgg
      601 tcacccagca gccattgccg gattcggcga ccagcgggta tgcccgtgtg aggctcgggg
      661 agacgaccgc ctcgtctcgc gcgatgatct cgcgcgcccg gggccccgga agctcggtct
      721 ggatacgggg ccgcgactgg cgggcagccg cggcggaggg agtggtctcg atgctcatcg
      781 gtaccagttg ctccggcgtg gagtggcgcg tcctgcccgc gcccctggct agacgccgac
      841 catcgtagca gcagcacccc ctcgagagcc cgatgggccg ccgccgcccg tcggtaagac
      901 gcgaaccgcc tcgcccacca ggcccgggcc gcggtagacg agccccgtcc acagctggac
      961 caggtcggca cccgcctcgt aggcgtcggc cacgtcctgc gcggacgcga tgccgcccga
     1021 ggcggccagc gccagctccg gaccggccag cgatcgcgcc agccgcagcc gatccagcag
     1081 gcgtgacatc agtggagccc cggagagccc gccggcctgc ccggcgtgcg gggatcggag
     1141 tgcatggcgc gcgacgctgg tgttcgacac gacgattccc accgcgccgg cgtccacggc
     1201 agcggccagc agccgggcga actcctcgtc gccgaggtcc ggtgagagct tgaccaggat
     1261 cggtcgctcc tggcgcaggc gccggcctgc cagcgacagc gcgccgacca ggtcgcgcag
     1321 cgccgccgca tcctgcaggt cacgcaggcc cggggtgttc ggggagctga cgttgaccac
     1381 caggtagtcg gccaccggcg ccaccaggcg atgcgccgcc aggtagtcat ccaccgcgtg
     1441 ctcgcgcggt gtcgctcgcc cccgccccaa gttgacgccg accacgaaat cgggcggcag
     1501 gtgcctacgg ctgagcatca ccctgcgggc caacgcagcc gcgccggcgt tgttgaaccc
     1561 catccggttg acgagggcct cgtcctccgt cagccggaac aggcgagggc gggggttgcc
     1621 gggctgagcc agcggcgtga tggtgccaac ctcggcgaag ccgaagccga gcgccgccca
     1681 gccgcgcagg gccaccgcat ccttgtcgaa gccggctccg acaccgatcc ggtttcggaa
     1741 gcgcaggccc gccaccagcg cccagggacg cgcccgccgc ggcgcgccgc cggccatggt
     1801 cagcaccggt cggccgaatc ggttcgcccc ggccatccgc agcgcgtcga gggtcagccg
     1861 gtggatgcgt tccggttcga aacggaagag cagcgggcgc gccgccgcgt agagcagtga
     1921 acgccagccg ctcactcgtc gtgcgcgcgg gggaacgcca tctcggtcgg gcgtaccggc
     1981 ggcaggggct cgccgccggc atcggtctcc acgccttcgg gagccatgaa ctcgtgccag
     2041 acctcggtgc cggccgcggg tgggtcgtcc agctccttga gcgccacgac gccctcgaac
     2101 cggatctggc cctgctcgcc ctcctcggtc tcgaactcgt cctcgtattc ctcggcgatc
     2161 tccagcgagc ggcccagcgc ggcctcctcg tcgtcgccgt ccaccaggat cacgcgatcc
     2221 tcgatcatga ccacgctctg cgactcgccg accaggtagg cgtagcgcag ctggaccccg
     2281 aaccagccgc tgggatcgac caccggctcc agctcgtcca gcgcgtccag ccggccgagc
     2341 tcatcgagct caccgagcga
//
LOCUS       DATACA010000304         3481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_520034, whole genome shotgun sequence.
ACCESSION   DATACA010000304 DATACA010000000
VERSION     DATACA010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520034"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHK28_06235"
     CDS             complement(<1..194)
                     /locus_tag="VHK28_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007259206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141861.1"
                     /translation="MSETELGYAPAKIARAARSPWADAGRALIRNPAGVAGLLIVGVL
                     ALLAILGPFIAPWDYRVQDIA"
     gene            complement(191..1123)
                     /locus_tag="VHK28_06240"
     CDS             complement(191..1123)
                     /locus_tag="VHK28_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141862.1"
                     /translation="MLTYAVRRALWLVPVLFVASLITFLIMHWAPGSPWLDWPPLDPE
                     TVAELDAQFGLNDPLHEQYLRWVGGVLRGDFGITTQFDPVPVRDVLAATLPISLHLGG
                     MAFALALLIGIPLGAIAALNHRTPVDYAATGIAILGMAAPSFALGIVLQLSLGHPPFH
                     EEIGFFPRTGWDGPEHWVLPTVALAALPAAQIARFTRASMLDVIHADYVRTAHSKGLP
                     EDRIVTRHMIRNALIPIATIAGPLMAVMLTGSIVVERLFEIPGMGNTFLFAIQFRDYT
                     TIMAIALIYAAVVAVANMVVDLAYGFIDPRIRDH"
     gene            complement(1253..1702)
                     /locus_tag="VHK28_06245"
     CDS             complement(1253..1702)
                     /locus_tag="VHK28_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141863.1"
                     /translation="MTACGDGELATLVDREGLSGAAVLRLDEGYAVAARARGGGAEVI
                     AFLRDEDAGWQVQRLAHAGSSDDELVQLISYGGETGEVWNTFVYGLAGPDVSRVVLEG
                     RPGPGGQVIDGAWVIALQQKDLAPADIRWRFLSASGGTLRTGSGITP"
     gene            complement(1735..2595)
                     /locus_tag="VHK28_06250"
     CDS             complement(1735..2595)
                     /locus_tag="VHK28_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141864.1"
                     /translation="MLRRLHASLLALAITIPLFIFGVPIVAARSPVVPMPGETVLSDA
                     ERRLARDTMTRHEREVVGPLLEGGEGLVVRYTRRLYRIVQLPDGSSRPEPVAPDRATP
                     GSALVLPELEVASSATKPGTDLYISFSVVRTRSSSPYEWQVFPYAEWRGWDGIDRSNS
                     SADTLAVAWAGNGYVHSQKGMGTLTDSWLCGKGPIDIWASDGTPNTGTSFSYHETGYM
                     WGCRAWWAAADIRIRQDGWRYRTDNLVYKYYHTYGGLQYSFTFSKSPGVSITPTKEQW
                     ALTVFATYRH"
     gene            complement(2765..3160)
                     /locus_tag="VHK28_06255"
     CDS             complement(2765..3160)
                     /locus_tag="VHK28_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141865.1"
                     /translation="MTDPSRAIRGARVGDLLPDVVEQASVRRNEATAILQPGPPGHLD
                     RYLRELDEARAEFEPWDGRVVSLQPDGQGDHRLLILDRYGQVYEVTDAPDAAALPDAH
                     NLAEWFRFLATACPECGVIDDPIGRGWVP"
     gene            complement(3190..>3481)
                     /locus_tag="VHK28_06260"
     CDS             complement(3190..>3481)
                     /locus_tag="VHK28_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141866.1"
                     /translation="DALGQDVPSDEERAGLGNIGEYRDAAASGQAGAEADSLAEEGLD
                     RLGDGDLGASQPIGAEEHASRVATTGGLDAGEASREGPGTTRDGQTNSPGTG"
BASE COUNT          549 a         1187 c         1237 g          508 t
ORIGIN      
        1 gcgatgtcct ggacgcggta atcccacggc gcgatgaacg ggcccaggat ggccagcagc
       61 gcgagcacac cgacgatcag caggccggcc acgccggccg gattgcggat cagcgcgcgg
      121 ccggcatcgg cccaaggcga gcgggcggcg cgcgcgatct tggccggcgc gtaccccagt
      181 tcggtctcgc tcaatggtcg cggatccgcg ggtcgatgaa gccgtaggcc agatccacga
      241 ccatgttggc cacggccacc accgcggcgt agatgagcgc gatggccatg atcgtggtgt
      301 agtcgcggaa ctggatggcg aacaggaagg tgttccccat gccggggatc tcgaacagtc
      361 gctcaaccac gatcgatccg gtgagcatga ccgccatcag cgggccggcg atggtggcga
      421 tcgggatcag cgcgttgcgg atcatgtggc gggtgacaat gcgatcctcg ggcaggccct
      481 tgctgtgcgc ggtgcgcacg taatcggcgt gaatgacgtc cagcatcgag gcgcgcgtga
      541 agcgagcgat ctgggcggcg ggaagcgccg ccagggcgac ggtgggcagc acccagtgct
      601 ctggcccgtc ccacccggtg cgcgggaaga agccgatctc ctcgtggaag ggtgggtggc
      661 ccagggacag ctggagaacg atgccgagcg cgaatgacgg cgcggccatg cccaggatgg
      721 cgatgccggt ggcggcgtag tccaccggcg tccggtggtt gagcgcggct atggctccca
      781 gcggaatccc gatcagcagg gccagggcga aggccatgcc gcccaggtgc aggctgatgg
      841 gcagcgtggc ggccagcacg tcacgcacgg gcaccggatc gaactgggtg gtgatcccga
      901 agtcgccgcg cagcacgccg cccacccagc gcaggtactg ctcgtgcagc ggatcgttca
      961 gcccgaactg ggcgtccagc tcggccaccg tctccgggtc cagcggcggc cagtcgagcc
     1021 agggacttcc cggcgcccaa tgcatgatca ggaaggtgat cagcgacgcg acaaacagca
     1081 ccggcaccag ccacagggcc cggcgcacgg cataggtcag caagcagcag ctcccgaaaa
     1141 ggcagaaatc ctcaggcgcg gcgcgccggg cggtgggcgt tccgccgaca gcacgtcgcc
     1201 actaccgtac taccccacct tcgggcagat atgttcagtc ttgccggccc cgtcaggggg
     1261 tgatgccgct gccggtgcgg agggtgccgc cgcttgccga gaggaaccgc cagcggatgt
     1321 cggccggagc cagatccttc tgctgaaggg caatcaccca cgccccgtcg atgacctggc
     1381 cgcccggccc cggcctgccc tcgagcacga ccctgctgac gtccgggccg gccaggccgt
     1441 agacgaacgt gttccacacc tcgccggtct cgccgccata gctgatcagc tgcacgagct
     1501 cgtcatcgct gctgccggca tgggccagcc gctggacctg ccagcccgcg tcctcgtccc
     1561 gcaggaaggc gatgacctcg gcgccgccgc cgcgtgccct ggcggcaacg gcgtagccct
     1621 catccaggcg gagcacggcc gccccgctca acccctcacg gtcgaccagc gtggcgagct
     1681 cgccgtcgcc gcaggcagtc accgccgcgg cgagcaggac gagggccgac agcctcagtg
     1741 ccggtaggtg gcgaacacgg tgagtgccca ttgctccttg gtgggcgtga tgctcacgcc
     1801 gggcgacttg ctgaaggtga agctgtactg aagtccaccg taggtgtggt agtacttgta
     1861 cacgaggtta tcggtccggt agcgccagcc atcctggcgg atgcggatgt cagccgccgc
     1921 ccaccacgca cggcagcccc acatgtagcc cgtctcgtga tacgagaacg aagtgccggt
     1981 gttcggcgtg ccgtcgctgg cccagatgtc gatgggtccc ttcccgcaca gccagctgtc
     2041 ggtcagggtg cccatcccct tctgggaatg gacatagccg ttgccggccc acgccaccgc
     2101 cagcgtatcg gccgatgagt tgctgcggtc gatgccatcc cagccgcgcc actcggcgta
     2161 cgggaagacc tgccactcgt acggggacga actgcgcgtg cggacgaccg agaacgagat
     2221 atagaggtcg gtgcccggct tggtggccga tgaggcgacc tccagctcgg gcagcaccag
     2281 cgcggaaccg ggcgtggcgc ggtcgggtgc caccggctcc gggcggctgg agccgtccgg
     2341 cagttgaacg atgcggtaca acctgcgggt gtagcgcacc accaggccct cgccgccctc
     2401 cagcagcggg ccgacgacct ctctctcgtg gcgcgtcatg gtgtcgcgcg ccaggcggcg
     2461 ctctgcgtca gacagcacgg tctcgcccgg catcggcacg accggcgacc tggccgccac
     2521 gatgggcaca ccaaagatga acagggggat ggtgatcgcg agcgccagca ggctcgcgtg
     2581 cagtcgccga agcatggcgg tctcctcaac agccggcccg gccacgtgtg cggcgggcac
     2641 tcagggaagg acggaactgc ggaatagacc ttggctgcgg cggctcacct cctgaccgcc
     2701 gcgcatgcta gccgacgagt gattactggc ggcttatcac cgccttaccg gcgttcgggc
     2761 ggtatcaggg cacccagccg cgtccgattg ggtcatcgat gacgccgcac tccgggcagg
     2821 ccgtggccag gaagcggaac cactcggcca ggttgtgggc gtcggggagg gcggcggcgt
     2881 cgggcgcatc ggtcacctcg tagacctgtc cgtagcgatc gaggatgagc aggcggtggt
     2941 caccctggcc atccggctgc aggctgacca cgcgcccgtc ccacggctcg aactcggcac
     3001 gggcctcgtc gagctcgcga aggtatcggt ccaggtggcc ggggggcccg ggctgcagga
     3061 tcgcggttgc ctcgttgcgg cgcacgctgg cctgctcgac cacgtcgggg agcaggtcac
     3121 caacgcgggc gccgcggatg gctcggctgg gatcggtcac gctgacgtgg atgctacgac
     3181 ggtggcgcct cagccggtcc ccggggagtt ggtttggcca tcgcgggtgg tgcctggccc
     3241 ctcgcgggat gcctcgcccg catccaggcc gccggtcgtt gccacgcggc tggcgtgctc
     3301 ttcggctccg atgggctggg atgcgccgag gtcaccatca cccaagcggt cgagtccctc
     3361 ttcggccagc gagtcggcct ccgcgccggc ctggccactg gcagccgcgt cccggtactc
     3421 gccgatgttt cccagcccgg ctcgttcctc gtcgctcggc acatcctgac cgagggcgtc
     3481 g
//
LOCUS       DATACA010000305         5107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_360123, whole genome shotgun sequence.
ACCESSION   DATACA010000305 DATACA010000000
VERSION     DATACA010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360123"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VHK28_06265"
     CDS             <1..608
                     /locus_tag="VHK28_06265"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEX2141867.1"
                     /translation="TVARGYGYGGVIINGRSYPTSALAGGICSTSTTLFNAAMRAGLE
                     IGERVNHSYYIERYPVGLDATVLKTDTSVTDMTFRNDTANPIVIRSYTGSGFVRFDIW
                     GVPDGRTVKLSNAVTSNHRAARDTTVVNTSLAPGTSRRIESPHNGFFSSVTRWVYDAD
                     GELLHQNVWESNYRVVNGIVEVGPAAAATSGEPDEPEDVAA"
     gene            660..2471
                     /gene="lepA"
                     /locus_tag="VHK28_06270"
     CDS             660..2471
                     /gene="lepA"
                     /locus_tag="VHK28_06270"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX2141868.1"
                     /translation="MIPPNRIRNFSIVAHIDHGKSTLADRLLEATHTIDKRLMQSQVL
                     DSMELEREKGITIKARAVRLHYDAADGQTYALNLIDTPGHVDFSYEVSRSLQACEGAI
                     LVVDASQGIEAQTLANVHLAMQQDLVIIPVINKIDLPSADPDMVIAELEDALAIPREE
                     VILASAKEGTGVDAILEAVVSRVPPPRGDDGARLQALVFDSHYDPYKGVVAYVRVANG
                     TLHDHERIRFMATDAQSDILELGYFRPQPVPTPSLTVGEVGYVATGLKSIAEARVGDT
                     LTDADKPATEPLPGYKQALPLVFAGIYPMRGDEYPLLRDALDKLHLNDASFVYEAESS
                     VALGFGFRCGFLGLLHMEIVQERLEREFDLDLIASAPSVEYRVKRLNKADELVVDNPA
                     QMPGPGEIEEISEPWVSVQVITPTDYIGPIMELATGRRGELKNMEYLDPKRVNLHMEM
                     PLAELIIDFYDQLKSRSSGYASLDYTFIGYRPGDLVKLDILVNAEPVDALSLIVHRSN
                     SYRAGKALVEKLKELIPRQMFEVPVQAAVGSHIISRETIRAMRKNVLAKCYGGDVTRK
                     RKLLEKQKAGKQRMKRIGSVEIPQEAFLAVLRVNEGG"
     gene            2472..3140
                     /locus_tag="VHK28_06275"
     CDS             2472..3140
                     /locus_tag="VHK28_06275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX2141869.1"
                     /translation="MDFEESLRLSIALGLTIFLVLLRFDSERIMRSDYFRYRFPWMGP
                     VSYYGLVVAFAIGIVVILPSGRSQLFLTPGDPDSMLPVMLLFSAVAVLNGIAFAFRWY
                     GGIMPLPTPLLPSRLFGAAASAVADELQFRSIVLGMLLFAEIPGGVPAAILIQALLYG
                     LAQRRLWRERQWYFLAGSVLLGYGAGLATVATGSVVPAMVGHFAVLAALYAFAGGRLR
                     TRPI"
     gene            3365..4558
                     /gene="hemW"
                     /locus_tag="VHK28_06280"
     CDS             3365..4558
                     /gene="hemW"
                     /locus_tag="VHK28_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX2141870.1"
                     /translation="MTAAATAANDSHHLYVHVPFCRLVCAYCDFVTVGGRASDIPLYV
                     DALLAELEARPAPGRLETIYLGGGTPSLLPAAEVGRIIEAATRRWAAAPIEVTLEANP
                     SGREAPDWQALRRAGTNRISLGVQSLRDADLQALARGHSADEARAAYAATRDAGFDNV
                     SVDLIYGIPGQSLDDWDANLRAAVDLQPDHLSLYALQLALDPDEWAAPPRPGALRWRR
                     RMAQRQDDDLAARQYVMAETVLRAAGYHHYELSSWARPGRESRHNSAYWSRRAYTGIG
                     AGAHSYDGAAQRAWNERDLDAYLSAVETRGWPRAGGEELDEPTRAFEAVALGLRRVDG
                     LSRGAFAAEFGADPTERYAAAVGDAAERGLLKVDLDSLRLTPAGRLMANDALIGFAPD
                     PVATG"
     gene            complement(4521..5072)
                     /locus_tag="VHK28_06285"
     CDS             complement(4521..5072)
                     /locus_tag="VHK28_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141871.1"
                     /translation="MRTFGALVNLALRAVILAILVEARRHPSDPRYAGKAIGTRGLVM
                     IPMSLLVPLAHALRRRHGRYPVWTDNLWLSVHALDLAGNHFDLYDRYRHFDAIPHAHG
                     TGAGTVVVAELLDMPAWSAVGLAQVGHIGLEAQEYYSDVWFGLRNVRGAWDTINDLLA
                     GAAGSLAYAGALSLWRRGRARSR"
BASE COUNT          797 a         1797 c         1742 g          771 t
ORIGIN      
        1 tcaccgtcgc tcgcggctac gggtacggcg gcgtcatcat caatgggcgc agttacccga
       61 ctagcgccct ggccggcggg atctgctcga cctccaccac cctgttcaac gctgccatgc
      121 gggctgggct ggagatcggc gagcgcgtga accactccta ctacattgag cgctacccgg
      181 tgggcctgga cgccacggtc ctcaagaccg atacgtccgt gaccgacatg accttccgca
      241 acgacaccgc caatccgatc gtgattcgct cgtacaccgg ttcgggcttc gttcgattcg
      301 acatctgggg cgtgccggac ggccggacgg tcaagctgtc caacgcggtc acctccaacc
      361 accgggcggc gcgcgacacg acggtcgtca acaccagcct ggcccccggc accagccggc
      421 ggatcgagtc gccacacaac ggcttcttct cgtccgtgac tcgctgggta tacgacgcgg
      481 acggcgagct cctccaccag aacgtctggg agagcaacta ccgcgtggtg aacggcatcg
      541 tggaagttgg accagccgcc gcggcaacct ccggcgagcc ggatgaaccg gaggacgtgg
      601 cggcctaacc gcttccgctc cgggcgcgag gcatcggtct ggcggtaccc ttccgccccg
      661 tgatcccccc gaaccgcatc cgcaacttca gcatcgtggc gcacatcgac cacggcaagt
      721 cgacgctcgc cgaccgcctg ctggaagcga cgcacaccat cgacaagcga ttgatgcagt
      781 cgcaggtgct cgacagcatg gagctggagc gcgagaaggg catcaccatc aaggcccggg
      841 cggtgcggct ccactacgac gcggccgacg gccagaccta cgcgctgaac ctgatcgata
      901 cccccggcca cgtcgacttc agttacgagg tgagccgctc cctgcaggcc tgcgagggcg
      961 ccatcctggt ggtcgacgcc agccagggga tcgaggccca gacgctcgca aacgtgcacc
     1021 tggccatgca gcaggacctc gtcatcattc cggtcatcaa caagatcgac ctgcccagcg
     1081 ccgaccccga catggtgatt gccgagctcg aggacgcgct ggccatcccg cgcgaggagg
     1141 tgatcctggc ctcggccaag gaggggaccg gggtggacgc catcctcgag gcggtggtca
     1201 gccgggtccc gccgccgcgc ggcgacgacg gtgcccgcct gcaggcgctg gtcttcgaca
     1261 gccactacga cccctacaag ggggtggtcg cctacgtgcg ggtggcgaac gggacgctgc
     1321 acgaccacga gcgcatccgg ttcatggcca ccgacgcgca gagcgacatc ctggagctcg
     1381 gctacttccg tccccagccg gtgcccaccc ccagcctgac cgtgggcgag gtgggctacg
     1441 tggcgaccgg cctgaagagc attgccgagg cgcgtgtggg cgacacgctg accgatgcgg
     1501 acaagcccgc caccgagccg ctgcccggct acaagcaggc gctgccgctg gtgttcgccg
     1561 gcatctaccc catgcgcggc gacgagtacc cgctgctacg cgacgcgctc gacaagctgc
     1621 acctcaacga cgccagcttc gtgtacgagg cggagtccag cgttgcgctg ggcttcggct
     1681 tccgctgcgg tttcctgggg ctgctgcaca tggagatcgt ccaggagcgc ctggagcgcg
     1741 agttcgacct ggacctgatc gcttcggcac catcggtcga gtaccgggtc aagcgcctga
     1801 acaaggccga tgagctggtc gtcgacaacc cggcccagat gcccgggccg ggcgagatcg
     1861 aggagatcag cgagccgtgg gtatcggtgc aggtgatcac gccgaccgac tacatcggtc
     1921 ccatcatgga gctggccacc ggtcgccgcg gcgagctgaa gaacatggag taccttgatc
     1981 cgaagcgcgt caacctgcac atggagatgc cgctggccga gctgatcatc gacttctacg
     2041 atcagctcaa gagccgctcg tcgggatacg ccagcctgga ctacacgttc atcggctacc
     2101 ggcccggcga cctggtgaag ctggacatcc tggtcaacgc cgagccggtg gacgccctct
     2161 ccctgatcgt ccatcggtcc aactcgtacc gcgccggcaa ggcgctggtc gagaagctca
     2221 aggagctgat cccgcgccag atgttcgaag ttccggtcca ggccgccgtc ggctcgcata
     2281 tcatcagccg tgagacgatc cgggccatgc gcaagaacgt gctggccaag tgctacggtg
     2341 gggacgtgac gcgcaagcgc aagctcctgg agaagcagaa ggccggaaag cagcggatga
     2401 agcgcatcgg ctcggtggag attccgcagg aggcgttcct ggcggtcctg cgcgtgaacg
     2461 agggcggcta gatggacttc gaggaatccc tgcgcctctc gatcgcgctg ggcctgacca
     2521 tcttccttgt cctgctgcgc ttcgactcgg agcggatcat gcgctccgac tacttccgct
     2581 accgcttccc gtggatggga ccggtcagct actacgggct ggtggtggcc ttcgccatcg
     2641 ggatcgtggt catcctgccc agcgggcggt cgcagctgtt cctgacgccc ggcgacccgg
     2701 actcgatgct gccggtcatg ctgctcttct cggcggtggc ggtgctcaac gggatcgcct
     2761 tcgccttccg ctggtacggc ggcatcatgc ccttgcccac gccgctgctg ccctcgcgac
     2821 tgttcggggc ggccgcctca gcggtggctg atgagctcca gttccgctcc atcgtgctcg
     2881 gcatgctgct cttcgccgag atccccggcg gggtgccggc cgccatcctg atccaggcct
     2941 tgctctacgg gctggcgcag cgacggcttt ggcgcgagcg gcagtggtac ttcctggcgg
     3001 gctccgtgct cctgggctac ggcgccgggc tggccaccgt ggcgaccggg tcggtcgtgc
     3061 ccgccatggt cggccacttc gcggtgctgg ccgcgctgta cgccttcgcc ggagggcgcc
     3121 tgcgcacacg tcccatctag ccacaggtgg cgcccgggtc gcttccgtgg acggctggcg
     3181 aggacgtgag aatgtcttcg ccacattcag atgaggcgcg catggaccga tggagacccc
     3241 gctcccaccg ccacccggcg cacctcagct gacagccggc aacagctggg cactctcggc
     3301 gactgcatgc gcacgtcgcc tgcatctggc gagaacggag cgcgcatgac accctaggcg
     3361 accgatgacc gccgccgcga ccgccgccaa cgattcccac cacctctatg tgcacgtgcc
     3421 gttctgtcgg ctggtgtgtg cctactgcga cttcgtcacg gtcggggggc gggcgtccga
     3481 catcccgctc tacgtcgacg cgctgctggc cgagctcgag gcgcgcccgg cgcccggccg
     3541 gctggagacg atctacctcg gtggcgggac gccctcgctg ctgccggcgg ccgaggtggg
     3601 ccgcatcatc gaggcggcaa cccgccgctg ggctgccgca ccgatcgagg tcacgctgga
     3661 ggcgaatccg agcggtcgcg aggcgcccga ttggcaggcg ctgcgccgcg ccggcacgaa
     3721 ccgcatctcg ctcggggtcc agtcgctgcg cgacgcggac ctccaggccc ttgcccgcgg
     3781 ccactccgcg gacgaggcgc gcgcggcgta cgcggcgaca cgggacgccg ggttcgacaa
     3841 cgtgagcgtg gacctcatct acggcatccc tggccagtcg ttagatgact gggatgcgaa
     3901 cctgcgggcc gccgtggacc tgcagccgga ccacctcagc ctgtacgccc tgcagctggc
     3961 actcgacccg gacgagtggg ccgccccgcc gcggcccggc gcgctgcgct ggcggcggcg
     4021 catggcccag cgccaggacg acgacctggc ggcgcggcag tacgtcatgg cggaaacggt
     4081 gctccgcgcg gcgggctacc accactacga gctctcctcc tgggcgcgtc ccggaaggga
     4141 atcgcgccac aactcggcgt actggtcgcg gcgggcgtac accggcatcg gcgccggggc
     4201 gcactcgtac gacggcgctg cccagcgcgc ttggaacgag cgcgacctcg acgcctacct
     4261 gtcggcggtc gagacgcgtg gctggccgcg agccggtggc gaggagctgg acgagccgac
     4321 gcgcgccttc gaagcggtgg cgctcggcct gcgccgggtc gacgggctga gccgcggggc
     4381 cttcgccgcg gagttcggtg cagacccaac tgaacgctac gccgcggcgg taggggacgc
     4441 ggccgagcgc ggcctgctga aggtggacct cgacagcctg cgcctcacgc cggccgggcg
     4501 gctgatggcc aacgatgccc tcatcggctt cgcgcccgac cccgtcgcca caggctgagc
     4561 gcgccggcat acgccagcga gccggccgcg ccggccagca ggtcgttgat cgtgtcccat
     4621 gcgccacgca cgttgcgcag cccgaaccac acgtcgctgt agtactcctg cgcctccagg
     4681 ccgatgtggc ccacctgggc cagcccgacg gcgctccagg caggcatgtc cagcagctcg
     4741 gccaccacca cggtgccggc gcccgttccg tgggcgtgcg ggatggcgtc gaagtggcgg
     4801 tatcggtcgt acaggtcgaa gtggttgccg gccaggtcca gggcatggac agagagccag
     4861 aggttatcgg tccacaccgg gtagcggccg tgacgccgac gcagcgcgtg ggccaggggc
     4921 accagcaggc tcatagggat catgaccagc ccccgggtgc cgatcgcctt gcccgcgtag
     4981 cgggggtccg acgggtggcg gcgtgcctcg accaggatgg ccaggatgac cgcgcgcagc
     5041 gccaggttga cgagcgcgcc gaaggtgcgc atgcgtccat gctaggaccc cgcggcttcg
     5101 ttgacac
//
LOCUS       DATACA010000306         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_923252, whole genome shotgun sequence.
ACCESSION   DATACA010000306 DATACA010000000
VERSION     DATACA010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923252"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VHK28_06290"
     CDS             <1..337
                     /locus_tag="VHK28_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEX2141872.1"
                     /translation="RTSSYHELTSAIVRQAGIAPRGRLEVDNIDAAKRMVEQRLGIAL
                     LPRTSVQAEVGGGRLVPVAITDMPPVRRQIVVARRRDAGEVTPVLDAFLSTLDDLRPT
                     EERAHRFLA"
     gene            812..1228
                     /locus_tag="VHK28_06295"
     CDS             812..1228
                     /locus_tag="VHK28_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX2141873.1"
                     /translation="MSTDPERAGRIPLLAALKPKDRQQVLRSARQRDYAAGQVIVSEG
                     DPATHLYLIAEGTARVERNDAGIVGRLRAGEFFGELGLIEEHGRTATVVADTNVTCLL
                     IPAWEFRASLEAHPEMAVPMLHAIIARLHAREHHPG"
     gene            complement(1230..1841)
                     /locus_tag="VHK28_06300"
     CDS             complement(1230..1841)
                     /locus_tag="VHK28_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX2141874.1"
                     /translation="MLSFLAAAGIAFGAVFVAEFGDKSQLLIFAFATRHPWRPVVAGL
                     VLAAAVIQGISVAVGAVVGAALPTTAVGIVAGVAFLAVAAWTLRGGEEGETTGDGGAE
                     GRVAAGLGGLGLVGTVAPTFIVGELGDKTMLATFALAASQGPIPTWIGTTAGEVAANL
                     VAVVVGRQMGARLPERALRLGSAALFALAGILVLGGALLGSGE"
     gene            1897..>3847
                     /gene="acs"
                     /locus_tag="VHK28_06305"
     CDS             1897..>3847
                     /gene="acs"
                     /locus_tag="VHK28_06305"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2141875.1"
                     /translation="MTTDERTTTDAIENLLREDRTFPPPPQFAANALLNDPGLLERAA
                     SEDGFREFWAHESKRIDWMEPWTELLDWQLPYAKWFVGGKLNVSVNCLDRHVAAGLGD
                     RVAYHWEGEPGDTRTVTYADLLEDTCRFANALRSLGVKRGDRVAIYMPMIPELPAAML
                     ACARIGAPHSVVFGGFSADALAGRIEDAECVAVITADGGYRKGASVPLKRNADEAIAR
                     TSSVQNCIVVRRTGDPVDWTDGRDHWWHELRESQATTADPEPMDSEDLLYLLYTSGTT
                     ARPKGIMHTTGGYLVGTATTHRYIFDIRPETDVYWCMADIGWVTGHSYIVYGPLANAS
                     TGIIYEGAIDFPDKDRWAQIAAKYRATILYTAPTAIRALMKYGHAEFERDDLSSLRLL
                     GSVGEPINPEAWAWYWRYVGGERCPVVDTWWQTETGMIMITPLPGITTLKPGSATFPF
                     PGVKADVVDEAGNSVPLGRGGYLVLERPWPAMLRGIYGDPERYEQTYWSRYPGRYFAG
                     DGCKRDDEGYYWLLGRVDDVMNVSGHRISTTEVESALVSHEAVAEAAVVGRTDPITGQ
                     GIFAYVILRSGIEPADPLAAELREHVADMIGKFARPKQIMFTPDLPKTRSGKIMRRLL
                     RDIAEGRALGDVTTLANADIVDSIKT"
BASE COUNT          614 a         1418 c         1286 g          529 t
ORIGIN      
        1 ccgcaccagc tcctaccacg agctgaccag cgccatcgtg cggcaggcgg gcatcgcgcc
       61 gcgcgggcgg ctggaggtcg acaacatcga cgccgcaaag cggatggtgg agcagcgcct
      121 ggggatcgca ctgctgccgc gcacctccgt ccaggctgag gtcggtggcg gccggctggt
      181 gcccgtggcc atcaccgaca tgccaccggt ccgtcgccag atcgtggtcg cccggcggcg
      241 ggatgcgggt gaggtgacgc cggtgctcga tgccttcctc tccacgctcg acgacctgcg
      301 gcccaccgag gagcgtgctc accgcttcct ggcttagggt aagtgacgcg tcgctggtgg
      361 cggccaccgg gtggctttaa cggccaagaa gccaccggcg gaggcgtctg cgtggccgtc
      421 cgggccaccg gatgagtgcg gcggctaggg acggcacgtc ttggcgggct gcttggctgt
      481 agtggccacc ccgcggccgc ggcggtccgc ccccgccact ggccgcttcg actctgccaa
      541 atgccggcgc ccgccggctt taaccctcga tctgccgcgc cttcttggcc atagagcgcc
      601 gctcggccgc ctcagccaga acgctcacgc ttgcgcgccg cctcccgcgc tccgacatcc
      661 aggctaagac cgcgtcccgg cagcgcctga cacgaagctt ttggcaggaa gaccgataag
      721 gccgcccgcc cgcggctcat ggccaaattg accccgcgac tcccactacg gctggccatc
      781 cacgcacccg ccgcactacg atgcgctgca tatgagcacc gaccccgagc gcgccggccg
      841 gatccccctt ctggcggccc tgaagcccaa ggatcgtcag caggtgctgc gcagcgcccg
      901 gcaacgtgac tacgccgcag gccaggtgat cgtgagcgag ggcgatcctg ccacccacct
      961 ctacctgatc gccgagggca cggcacgcgt cgagcgcaac gatgcgggca tcgtcggccg
     1021 cctgcgcgcg ggcgagttct tcggcgagct ggggctgatc gaggagcacg gtcgcacggc
     1081 aacggtcgtg gccgacacaa acgtcacctg cctgctgatc cccgcgtggg agttccgcgc
     1141 ctccctggag gcacacccgg agatggcggt ccccatgctg cacgccatca tcgcccgcct
     1201 ccacgctcgc gagcaccacc cgggctgacc tactcgccgc tgcccagcag ggcgccgccg
     1261 agcaccagga tcccggccag ggcgaacagc gccgcagacc cgagccgtag tgcgcgctcc
     1321 gggagccgcg ctcccatctg gcgccccacc accaccgcca ccaggttggc cgcgacctcg
     1381 ccagcggtcg tcccgatcca ggtcgggatc ggtccctggc tggcggccag ggcgaacgtg
     1441 gcgagcatgg tcttgtcgcc gagctcgccg acgatgaagg tgggcgccac cgtccccacc
     1501 aggcccagcc ccccgagccc ggcggcaacc cgcccctcgg cgccgccgtc acccgtagtc
     1561 tccccctcct ccccaccgcg cagcgtccag gccgccaccg ccaggaacgc gacgccggcc
     1621 acgatcccga ccgccgtggt cggcagcgcc gcgcccacca ccgcgccaac cgccacgctg
     1681 atgccctgga tgaccgccgc cgccaggact aggccggcga cgaccggccg ccatggatga
     1741 cgggtggcga aggcgaagat gaggagctgg ctcttgtcgc cgaactcggc cacgaacacg
     1801 gccccgaagg caatgccggc ggcggccagg aaggaaagca tgggtggtca atgctacccg
     1861 ggtcgcccca tgggacccgg gccgtagaat cggtccatga cgaccgacga gcgcacgacg
     1921 accgacgcga tcgagaacct cctccgcgaa gaccgcacct tcccgccgcc accgcagttc
     1981 gcggcgaacg ccctcctcaa cgatcccggc ctcctggagc gcgccgcctc cgaggatgga
     2041 tttcgcgagt tctgggccca cgagtcgaag cgcattgatt ggatggagcc ctggacggag
     2101 ctgctggact ggcagctgcc ctacgccaag tggttcgtgg gcggcaagct caacgtcagt
     2161 gttaactgcc tggaccgcca cgtcgcagcc ggcctgggcg accgggtggc ctatcactgg
     2221 gagggcgagc ctggcgacac ccgcaccgtc acctatgcgg accttttgga ggatacctgc
     2281 cgcttcgcca acgcccttcg ctcgctgggc gtgaagcgcg gggatcgggt ggcgatctac
     2341 atgcccatga tccccgagct gccggccgcc atgctggcct gcgcccggat cggcgccccg
     2401 cacagcgtcg tcttcggcgg cttctcggcc gacgccctgg ccggccgcat cgaggacgcg
     2461 gagtgcgtgg cggtcatcac cgcagacggg ggctaccgca agggcgcatc ggtcccgctc
     2521 aagcgcaacg cggacgaggc gatcgcccgg acgtcatcgg tccagaactg catcgtggtg
     2581 cgccgcacgg gggatccggt tgactggacc gatgggcgcg accactggtg gcacgagctc
     2641 cgcgagtcgc aggcgaccac cgcggacccg gagccgatgg attccgagga cctcctgtac
     2701 ctgctctaca cctcggggac caccgcccgg ccgaagggca tcatgcacac caccggcggc
     2761 tacctggttg gcaccgccac cacccatcgg tacatcttcg acatccgacc ggagaccgat
     2821 gtctactggt gcatggcgga catcggctgg gtgaccgggc actcgtacat cgtgtacggg
     2881 ccgctggcca atgccagcac cgggatcatc tacgaggggg ccatcgactt cccggacaag
     2941 gaccgatggg cccagatagc agccaagtac cgcgccacca tcctgtacac cgcaccgacc
     3001 gccatccgcg ccctgatgaa gtacggccac gccgagttcg agcgcgacga cctgtcgtca
     3061 ctgcgcctgc tgggcagcgt gggggagcca atcaacccgg aggcctgggc ctggtactgg
     3121 cgatacgtcg gcggcgaacg ctgcccggtg gtcgacacct ggtggcagac cgagacggga
     3181 atgatcatga tcaccccgct gccgggcatc accaccctga agccgggcag cgccaccttc
     3241 ccgttccccg gggtgaaggc ggatgtggtg gacgaggcgg gcaactcggt gccgctgggg
     3301 cgtggcggct accttgtgct ggagcggccg tggcccgcca tgctgcgcgg catctacggc
     3361 gacccggagc gctacgagca gacctactgg agccgctacc cgggacgcta ctttgccggc
     3421 gacggctgca agcgcgacga cgagggctac tactggctgc ttggccgcgt ggacgacgtg
     3481 atgaacgtct cgggccatcg catctctacc accgaggtgg agtcggcgct ggtctcgcac
     3541 gaggccgtgg cggaggccgc ggtggtgggc cgcacggacc ccatcaccgg ccagggcatc
     3601 ttcgcctacg tgatcctgcg ctccgggatc gagcccgccg acccgctggc agccgagctg
     3661 cgggagcacg tggccgacat gatcggcaag ttcgcgcggc ccaagcagat catgttcacg
     3721 ccggacctgc ccaagacgcg ctccggcaag atcatgcggc ggctgctgcg cgacatcgcc
     3781 gagggtcgtg cgctgggcga cgtcaccacg ctcgcgaacg ccgacatcgt cgactcgatc
     3841 aagacgc
//
LOCUS       DATACA010000307         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_520228, whole genome shotgun sequence.
ACCESSION   DATACA010000307 DATACA010000000
VERSION     DATACA010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520228"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VHK28_06310"
     CDS             <1..233
                     /locus_tag="VHK28_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141876.1"
                     /translation="FIAPNATTDTSVYPTENDRKAAELFTSGTAVYDLDDAIGGEIQS
                     VEREQLVSLIQSGDVDAFIQAMMDVTERVRGG"
     gene            251..1252
                     /locus_tag="VHK28_06315"
     CDS             251..1252
                     /locus_tag="VHK28_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2141877.1"
                     /translation="MAASQPRARSTPQRAGGWTVAYLFVLPALAVFALFYLWPALNTI
                     VSSFFTWSILAPWQAHDPDSWRFAGLGNYVEVLTDPRFWNAALNTLIWLVVFPLLATF
                     LSLLIALLVWEVGRGARLFRTIFVLPMTVSLTAAGVLWVLVFNPQFGVLSAALGAIGL
                     SGAELNAGPLNVRLGDWLSNPGSLELGPVTISFVNLSAILPAVWALTGFGVISITAGL
                     TSISPELIEAATVEGAGRYRIARDVVIPQLRGALVIVAVVFVIYALRTFDVVWVTTQG
                     GPGTDSEVVAILLWRKALAFFDTPSAGEATAIAVLLSAVMVIGAYPYLRSLLREAPQ"
     gene            1249..2091
                     /locus_tag="VHK28_06320"
     CDS             1249..2091
                     /locus_tag="VHK28_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2141878.1"
                     /translation="MSRAGGMGSVWWMYGLLTVLAILWMVPVVTVVMSALRPLGEIRD
                     GWWNLAGASFTLDNFLGAWNEADLGSKAINSLIITGFSVITTIAIGALAAYAFVRLRF
                     RGRGLIYFLLISTMIVPVQIILIPLLPWFRTLGFAEGAWQPFLGMVLVHAAFGLGWAV
                     FMMGSFMAAVPVELTEAGQIDGADHFGIFRRIVLPLALPGIVSFAIIDFVFVWNDLLL
                     GLTLLDADHAPLTVGIANLQSAHLAQQDVISAGALLAILPPLALFVALNRYYVRGLFS
                     GGVK"
     gene            2187..>3701
                     /locus_tag="VHK28_06325"
     CDS             2187..>3701
                     /locus_tag="VHK28_06325"
                     /inference="COORDINATES: protein motif:HMM:NF018528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEX2141879.1"
                     /translation="MVHPKHGVLELTGAPPFDEAHRGDPVATRAYRAVLASDASACCW
                     LEGPDGQRCTDASLDATDPAHPRWTGHLAGAKVEATAVATAPERFELQWRLAGWRETR
                     PPRVRFAGRLALPALAEITDTDPPDAVAVRQVTLAKGSTLSVTAPDMGATARLTQPAG
                     SWVADGNGFAADFEGVATVLAISLDGPCANRRDGTWSAPPGTLVPDPITAKALAYVRC
                     CTALRTGPDERVLLTDHRLLPLSWTRDAYYQALLLLATGTDADLDVVADHLRWLWRRC
                     DRPDGTWARSHHADGRRKDLGFQADQQLYPVLEACDYWRVTRRLPEGLDWTDAIAGCW
                     AAVQDQVDPATGLLASVENAADDPARAPFIGSSQVLLWYTALRLAEVAEAGDIRLDPG
                     ELRSVASRARTAFAAHFEHLGAWPYAVDGHGARIAYHDANDLPLALAPLWGFCDAGEP
                     GWRDTVAFAWSEANNGWFSGERGGLGSIHTPGPWTLGDVQDWLVGRITDDAPRRD"
BASE COUNT          488 a         1296 c         1256 g          661 t
ORIGIN      
        1 ggttcatcgc gccgaacgcg acgacggata catcggtcta tccgactgag aacgaccgca
       61 aagcggccga gttgttcaca tcgggcaccg cggtctacga cctggacgac gccattggtg
      121 gcgagatcca gtcggtggag cgcgagcagc tggtgtccct catccagagt ggggacgtgg
      181 acgccttcat ccaggccatg atggacgtga ccgaacgagt acgaggcggg tgagcacctg
      241 aggcgccgaa atggctgcca gccagccccg ggcgaggtca acgccacagc gcgcgggagg
      301 atggacggtc gcgtacctgt tcgtcctccc cgcgctggcg gtgttcgcgc tcttctacct
      361 gtggccggcg ctcaacacca tcgtctccag cttcttcacc tggagcatcc tggcgccctg
      421 gcaggcgcat gacccggaca gctggcggtt cgccgggctg ggcaactacg tcgaggtcct
      481 gaccgacccg cgcttctgga atgccgcgct caacacgctc atctggctgg tcgtcttccc
      541 gctgctggcg accttcctct ccctcctgat cgcgctcctg gtctgggagg tgggccgcgg
      601 tgcgcgcctg ttccgcacca tcttcgtgct gcccatgacc gtgtcgttga ccgccgcggg
      661 cgtgctgtgg gtgctcgtct tcaacccgca gttcggggtg ctgtccgccg ccttgggcgc
      721 catcggcctc agtggtgccg agctcaatgc cggccccctg aacgtgcggt tgggcgactg
      781 gctgtcgaat ccgggaagcc tggagctggg gccggtcacc atcagcttcg tcaacctgag
      841 cgccatcctg ccggccgtgt gggctctcac cggtttcggg gtgatcagca tcaccgctgg
      901 cctgaccagc atcagcccgg agctgatcga ggcggccacg gtggagggag ccggccgcta
      961 ccgcatcgcg cgtgacgtgg tcatccccca gctgcggggc gccctggtga tcgtcgccgt
     1021 cgtgttcgtc atctacgccc tgcgcacctt cgacgtggtg tgggtcacca cccagggtgg
     1081 accgggtacc gacagcgagg tggtggccat cctgctgtgg cgcaaggcgc tggccttctt
     1141 cgacacgccc tccgccggcg aggcgaccgc catcgcggtg ctgctcagtg cggtgatggt
     1201 catcggtgcc tacccctacc tgcgcagcct gctcagggag gcgccgcaat gagccgagcg
     1261 ggcggcatgg gctccgtgtg gtggatgtac gggctgctga cggtgctcgc catcctgtgg
     1321 atggtgcccg tggtcacggt ggtgatgtcc gcgctgcgcc cgcttggtga gattcgcgac
     1381 ggctggtgga acctggcggg cgcgtccttc accctcgaca acttcctggg cgcctggaac
     1441 gaggccgacc tgggcagcaa ggccatcaac agcctgatca tcaccggctt cagcgtgatc
     1501 accacgatcg cgatcggcgc gctggcggcg tacgccttcg tgcgccttcg attccgggga
     1561 cgcgggctga tctacttcct gctgatcagc accatgatcg tgccggtgca gatcatcctg
     1621 atcccgctgc tgccctggtt ccgcacgctg ggctttgccg agggcgcctg gcagccgttc
     1681 cttggcatgg tgctggtgca cgccgccttc ggcctgggct gggccgtgtt catgatgggt
     1741 tcgttcatgg cggccgtgcc ggtggagctg accgaggccg gccagatcga tggcgccgat
     1801 cacttcggca tctttcggcg catcgtgctg cccctggccc tgcccggcat cgtcagcttc
     1861 gccatcatcg atttcgtctt cgtctggaac gacctgctgc tggggttgac cttgcttgac
     1921 gcggatcacg cgccgctgac ggtgggcatc gccaacctgc agtcggcgca cctggctcag
     1981 caggacgtca tcagcgccgg tgccctgctg gccatcctgc cgccgctggc gctgttcgtg
     2041 gcgctcaacc gctactacgt ccggggcctc ttctccggcg gggttaagtg agaggggcgc
     2101 ccgcgaacgc caagccggtc gacctgggat ccggcttggt cgccgcctcg tttgggttcg
     2161 gagagccatc gctcctgtcg ctgaccatgg tccacccgaa gcacggcgtg ctggagctga
     2221 ccggcgcgcc gcccttcgac gaggcacatc gcggcgaccc ggtagctact cgcgcgtatc
     2281 gggctgtgct cgcctccgac gcttctgcct gctgctggct ggagggtccg gacggacaac
     2341 gctgcaccga tgcctctctc gacgcgaccg acccggctca ccctcgctgg accgggcatc
     2401 tggccggcgc caaggtagag gcgacggcgg tggccaccgc accggagcgg ttcgagcttc
     2461 agtggcgcct agcggggtgg cgcgagacgc ggccgccgcg ggtccgcttc gccggacggc
     2521 tggcactgcc cgccctggcc gagatcaccg acaccgatcc gcccgacgcc gttgcggtgc
     2581 ggcaagtgac gctggccaag ggatccacgc tgtcggtgac cgcgcccgac atgggcgcga
     2641 ccgctcgact gacgcagccc gccggttcct gggtggccga cgggaatggc tttgccgctg
     2701 acttcgaggg ggtcgccacg gtcttggcga tcagccttga cggcccctgc gccaatcgcc
     2761 gcgacgggac atggtccgca cctccgggga ccctcgtccc tgaccccatc acggccaagg
     2821 cgctggccta cgtccgttgc tgtaccgcac tgcgcaccgg ccccgatgag cgagtcttgc
     2881 tcaccgatca ccgcctcctg ccgttgtcct ggacgcgaga cgcctactac caggcgctgc
     2941 tgctgctggc caccgggacc gatgcggacc tcgacgtcgt ggccgaccac ctgcgctggc
     3001 tgtggcgccg ctgcgaccgt ccggatggca cctgggcgcg cagccatcac gccgacggcc
     3061 gccgcaagga cctcggtttt caggcagacc agcagctcta tccggtgctc gaagcgtgcg
     3121 actactggcg cgtgacacga cgccttcccg aagggctgga ttggaccgat gccattgccg
     3181 gctgctgggc ggccgtgcag gaccaagtgg acccggccac cggcctcctg gcctccgtcg
     3241 agaatgctgc cgatgatcct gcccgggccc ccttcatcgg ctccagccag gtcctgctgt
     3301 ggtacacggc ccttcgcctg gcagaggtgg ctgaggcggg cgacattcgc ctagaccccg
     3361 gggagctgcg gtccgtcgcc agccgcgcgc gcaccgcgtt cgccgcgcac ttcgagcatc
     3421 tcggcgcctg gccgtacgcg gttgatggtc acggcgcacg catcgcctac cacgacgcga
     3481 acgaccttcc gctggcactg gccccgctct ggggcttctg cgatgcgggt gagcctggct
     3541 ggcgcgacac ggtcgccttc gcctggtcgg aggccaacaa cggctggttc tccggcgagc
     3601 ggggcgggct gggctcgatc cacacgcctg gcccatggac gctgggcgac gtccaggact
     3661 ggctggtcgg gcggatcacc gacgacgctc cccgccgcga t
//
LOCUS       DATACA010000308         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1083525, whole genome shotgun sequence.
ACCESSION   DATACA010000308 DATACA010000000
VERSION     DATACA010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083525"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="mazG"
                     /locus_tag="VHK28_06330"
     CDS             <1..1035
                     /gene="mazG"
                     /locus_tag="VHK28_06330"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX2141880.1"
                     /translation="VPFDPGLPLLILPGSRPPSDGVPAVLPGRHARRSARKVLAALYP
                     QSHRLRPLPAGEPRPLESLSDEELQAGSWLVPALPPLDNLASPHGMAAISARLRAPDG
                     CPWDRRQTHASLRPFALEEAYELVDAIERGSASDLAEELGDLFLQVILHAQLAAEESA
                     FDLTDVYRTLGAKIVRRHPHVFGDLEVADAAEVIRNWESIKASERSDAGAATSPFAAV
                     ARALPALAASREIQERASSLGWDWDSVDGVWEKVREEIDELHAAGSLPERERHDAALH
                     ELGDVLFALVNMGRWLKVDPEEALRAANRRWIERYERVEALATSRGVVLGDLSLAEKD
                     LLWDEVKAAR"
     gene            1032..>1546
                     /locus_tag="VHK28_06335"
     CDS             1032..>1546
                     /locus_tag="VHK28_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2141881.1"
                     /translation="MSRVAMVVHAIYPGDPRIRRQSDALTDAGHEVDIFCLRQRGEPA
                     EEASGRVRIIRLPINRAFTGFAGHMAEYFAFAGLATLRLAREHRRRRYDLVQVATVPD
                     FLAFAAAPVKLAGVPLLLDLHEDMPEFFRDRFAHPVLRPLMPLVSGTARGAAAFATEI
                     ITVHEPLRQLSV"
BASE COUNT          197 a          577 c          542 g          230 t
ORIGIN      
        1 gtccccttcg atcccggcct gccgctgctg atcctgcccg gctcgcgacc gccgtccgat
       61 ggcgtgccag cggtcctgcc cgggcggcat gcgcgacgct cggcgcgcaa ggtgctggcc
      121 gcgctgtacc cgcagtccca ccgcctgcga ccgctgccgg ccggcgagcc cagaccgctc
      181 gagtcgctct ccgacgagga gctgcaggcc ggcagctggt tggtgcctgc cctgccgccg
      241 ctggacaacc tggcctcgcc gcacggcatg gcagccatca gcgcccggct gcgcgcgcct
      301 gacggctgtc cctgggaccg tcgccagaca cacgcctccc tgcggccctt cgcgctggag
      361 gaggcgtacg agctggtgga cgccatcgag cgcggatcgg cgtcggacct ggccgaggag
      421 ctcggcgacc tcttcctgca ggtcatcctg catgctcagc tggctgccga ggagtccgcg
      481 ttcgacctga ccgatgtcta ccgcaccctc ggcgccaaga tcgttcgccg ccatccccac
      541 gtgttcggcg atctcgaggt ggccgatgcg gccgaggtga tccgcaactg ggagtcgatc
      601 aaggcctccg aacggagcga tgcgggcgcc gcgacctccc ccttcgccgc cgtggcccgc
      661 gcgctgcccg ccctggcggc cagccgcgag atccaggagc gcgcatcctc gctcggctgg
      721 gactgggact ctgtcgacgg ggtctgggag aaggtgcgcg aggagatcga cgagctgcac
      781 gccgccggat cgctgcccga gcgcgagcgc cacgacgcag ccctgcacga gctcggcgac
      841 gtgctgttcg ccctggtcaa catgggacgc tggctgaagg ttgacccgga ggaggcgctg
      901 cgcgccgcca accgacgctg gatcgagcgc tacgagcgcg tggaggcgct ggccacctcg
      961 cggggcgtgg tgctcggcga cctgtcgctg gccgagaagg acctgctgtg ggacgaggtc
     1021 aaggcggcac ggtgagccgg gtcgccatgg tggtgcacgc catctacccg ggtgatccac
     1081 gcattcgtcg ccagagcgac gccctgaccg atgccggcca cgaggtcgac atcttctgcc
     1141 tgcgacagcg cggcgagccg gccgaggagg cgagcggccg ggtgcgcatc atccgcctgc
     1201 cgatcaaccg cgccttcacc ggcttcgccg ggcacatggc cgagtacttc gccttcgccg
     1261 gccttgccac tctgcggctg gcgcgggagc atcgccgtcg gcggtacgac ctggtgcagg
     1321 tggccaccgt gccggacttc ctggccttcg ccgccgcgcc ggtcaagctg gccggcgtgc
     1381 cgctgctgct cgacctgcac gaggacatgc ccgagttctt ccgcgaccgc ttcgcgcacc
     1441 cggtgctgcg cccgctgatg ccgctggtga gcggcaccgc ccgcggtgcg gccgccttcg
     1501 ccaccgagat catcaccgtc cacgagccgc tgcgccagct gtcggt
//
LOCUS       DATACA010000309         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2770000, whole genome shotgun sequence.
ACCESSION   DATACA010000309 DATACA010000000
VERSION     DATACA010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2770000"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1753)
                     /locus_tag="VHK28_06340"
     CDS             complement(77..1753)
                     /locus_tag="VHK28_06340"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX2141882.1"
                     /translation="MIEAVGEEGTDLVIFGWGGPPTPAAVARAEAEGRRPRKDPPVFS
                     PTIDAVVRESPCDIAVVKQRGVEEVRSILVPVRGGPHAELAMRLARELGRRFKAHVVV
                     MHIVPKGVGEVALRREQEALDRFVREHGGGRKVQGILREATSVRQAIIRESANHNLVV
                     MGASAHPSNAAADGRFLFGVVADSVASRAKPTVIVVKTKQRVGLATFEELRAAEGTLA
                     AADAYAERTNSLPTVVDRWFAENTFHASEFRDIRRLVELKEKHGLTVSVGLPALNEEK
                     TIGLVIRRIKSALMDRMPLIDQMVVVDSDSTDRTRQVAEDLGVLVIRHSEILPEAGTH
                     VGKGEALWKSLHVLDGDIVTWIDTDISNIQPRFVYGLIGPLLREPRIQYVKGFYKRPI
                     KQGDRLVPEGGGRVTELMARPFLNLFFPELSGMIQPLSGEYAGRRELLESVPFYTGYS
                     VEIGLLIDILEASGLSAIGQVDLERRIHRNQPLGNLSQMSYVILQGAFRKLEERHRIE
                     LLTEMGRGMKLIIQEKDRFSLEVREIGDELRPPMRTIPAYVERRRALKRG"
     gene            complement(1750..>1981)
                     /locus_tag="VHK28_06345"
     CDS             complement(1750..>1981)
                     /locus_tag="VHK28_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141883.1"
                     /translation="HRPGAHQEQQLAALTPLYFAPTAGFIAETLDMTTDQAERVIDAQ
                     ARTARRLLQRVLDFGDEEGVEIRTMVRIGRTA"
BASE COUNT          278 a          717 c          677 g          309 t
ORIGIN      
        1 cgtcgcccgc atcggggaaa gcgtggagat gctgccgcac cggcgtggca tcccgggcgc
       61 ggaccgactg ccgcggtcag ccgcgcttca gggcccggcg gcgctcgacg taggcgggga
      121 tggtgcgcat cggggggcgc agctcatcgc cgatctcgcg cacctcgagg ctgaatcggt
      181 ccttctcctg gatgatgagc ttcatgccgc gccccatctc ggtgagcagc tcgatccggt
      241 ggcgctcctc cagcttgcgg aaggcaccct gcaggatcac gtacgacatc tgcgacaggt
      301 tgcccagcgg ctggttgcgg tggatgcgcc gctccaggtc gacctggccg atggccgaca
      361 gcccggacgc ctccaggatg tcgatcagca gcccgatctc caccgagtat ccggtgtaga
      421 acggcaccga ctcgagcagc tcccggcgcc cggcgtactc gccggagagc ggctggatca
      481 tgcccgacag ctcggggaag aacaggttca ggaagggtcg cgccatcagc tcggtgaccc
      541 ggccgccgcc ctccggcacc aggcggtcgc cctgcttgat cggtcgcttg tagaaaccct
      601 tcacgtactg gatgcgcggc tcgcgcagca atggaccgat gagcccgtac acgaagcgcg
      661 gctggatgtt gctgatgtcg gtgtcgatcc aggtcacgat gtcgccgtcg agcacgtgca
      721 ggctcttcca cagcgcctcg cccttgccca cgtgggtgcc cgcctcgggc aggatctcgc
      781 tgtgccgtat caccagcacg cccaggtcct ccgcgacctg gcgggtgcgg tcggtggagt
      841 cggagtcgac caccaccatc tggtcgatca gcggcatgcg gtccatcagc gcgctcttga
      901 tgcgccggat gaccagcccg atggtcttct cctcgttcag agccggcagc ccgacgctga
      961 ccgtcaggcc gtgcttctcc ttgagctcca ccaaccggcg gatgtcgcgg aactcggagg
     1021 cgtggaacgt gttctcggcg aaccatcggt ccaccacggt ggggagggag ttggtgcgct
     1081 ccgcgtaggc gtccgccgcg gccagggtgc cctccgcggc ccgcagctcc tcgaaggtgg
     1141 ccagcccgac gcgctgcttg gtcttgacca cgatcaccgt cggcttggcg cgcgacgcca
     1201 ccgagtcggc caccaccccg aacaggaagc gcccgtcggc cgccgcgttc gacggatggg
     1261 cagaggcgcc catcaccacc aggttgtggt tcgccgactc gcggatgatg gcctgtcgga
     1321 ccgatgtcgc ctcgcgcagg atgccctgca ccttgcgccc gcctccgtgc tcgcgcacga
     1381 agcggtcgag cgcctcctgc tcgcggcgca gtgccacctc gcccacgccc ttgggcacga
     1441 tgtgcatgac caccacgtgg gccttgaacc gccgtcccag ctcgcgggcc aggcgcatgg
     1501 ccagctcagc gtgcggcccg ccgcgcaccg gcaccaggat ggagcgcacc tcctccacgc
     1561 cacgctgctt gacgaccgcg atgtcgcagg gcgactcgcg cacgaccgcg tcaatggtcg
     1621 gcgagaagac cggcgggtcc ttgcgcggtc gccggccctc ggcctcggca cgggccaccg
     1681 cggcgggcgt cggcgggccg ccccagccga agatgaccag gtcggtgccc tcctcgccga
     1741 ccgcctcgat cacgccgtgc ggccgatgcg gaccatggtc cggatctcga cgccctcctc
     1801 gtcgccgaag tcgagcacgc gctgcagcag ccgtcgggcg gtgcgggcct gggcgtcgat
     1861 cacccgctcg gcctggtcgg tcgtcatgtc cagcgtctcg gcaatgaagc cggccgtcgg
     1921 cgcgaagtac agcggtgtga gggccgccag ctgctgctcc tggtgcgccc cgggtcggtg
     1981 g
//
LOCUS       DATACA010000310         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1244436, whole genome shotgun sequence.
ACCESSION   DATACA010000310 DATACA010000000
VERSION     DATACA010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244436"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..808)
                     /locus_tag="VHK28_06350"
     CDS             complement(101..808)
                     /locus_tag="VHK28_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017831785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141884.1"
                     /translation="MAQPRDEVETAARLDRAEVLAARLDRPMGVLGLLFVFVVLGQML
                     ATQPGLLTFLSVLGWLFWGIFVAEFLLRAYIARFQRRFWKRNWWQVIFLLVPFLRFFR
                     ALQGLRVFRAARLARFGSVLSAGIRGGRSAGRLLSSRIGWLAAVTAMVIMVSSQLLYV
                     SGSYERYGDALHAAAMSTITGNELTATDGFARLLEVLLAVYSVAVFATLAGAVGAFFL
                     RGSGAEEPTLPNAEGNS"
     gene            1038..2153
                     /locus_tag="VHK28_06355"
     CDS             1038..2153
                     /locus_tag="VHK28_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2141885.1"
                     /translation="MRAAVPAGSGGRGRGSGTEPSRNWRRTCAAAAAAVVLLAGCTAT
                     DQEPGTQTEGAVSSPAAGQAPEQGSSGGVSIPDIVASVEPSVVTIFTERGGLGSGVIY
                     SADGLILTNEHVVRGADEVEVGFADGKREAGTVVAEDPVTDLALVQVERTGLPAAEFE
                     PSLPRVGELAVVIGSPLGFANTATAGIISGLHREIPGSASNSQSLVDLIQTDAPISPG
                     NSGGAVVNSEGKVIGISEAYIPPQAGAVALGFAIPAANAMEVAEQLRDDGSAEHAFMG
                     LGPAAITPQIAEQLGLPDTQGALVLSVVDGGPAAEAGLEPGDVIVGLGGEEIQTPEDL
                     LAALRGHAPGDEVEVEISRGGEPSQITVTLADRPSAE"
     gene            2243..>2516
                     /locus_tag="VHK28_06360"
     CDS             2243..>2516
                     /locus_tag="VHK28_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141886.1"
                     /translation="MTGVPLTKIPVSILDRANSRSGGTEAHALRATAIRARRAEELGY
                     HRFWVAEHHGVPGIAGSAPTVLMGVIAAETERIRIGSGGVMLPNHQP"
BASE COUNT          488 a          773 c          866 g          389 t
ORIGIN      
        1 ccatatccag ttcagtgctc atactctctc ccaccgctcg tcagcgccaa ataccggcgt
       61 cggccgctta atctctatcc tatggccgcg tcatgccggt ctaggagttg ccctcggcgt
      121 taggcagcgt cggttcttcg gcgccgctgc cacggaggaa gaatgcacca acggcgcctg
      181 ccaaggtggc gaaaacggct accgaataga cggccagcag cacttccagc agccgcgcga
      241 agccatccgt ggcagtcaac tcgttgccgg taatggtcga catggccgct gcatgcaggg
      301 catcgccata ccgctcatag gaaccggaga catagagcaa ctggctggag accatgatca
      361 ccatggctgt gaccgctgcg agccagccga tccggctcga aagcagcctt ccggctgaac
      421 gcccaccgcg gataccggcc gagagcacgc tgccgaatct ggccaagcgt gccgcccgaa
      481 acacccggag cccctgcagg gcacggaaaa aacgcaggaa tggcaccaac aggaaaatga
      541 cctgccacca gttgcgcttc cagaaccggc gctggaatct ggcgatgtac gcgcgcagca
      601 ggaattccgc gacgaaaatg ccccagaaca gccagccaag aaccgacagg aacgtcagca
      661 gcccgggctg ggtggccagc atctggccga ggacgacgaa cacaaacagc agtcccaaga
      721 cgcccatcgg ccggtcgagc cgggcggcca gcacctcggc acggtccagg cgggccgctg
      781 tttccacttc gtcgcgtggc tgcgccatgt atcctccagc tcgtccatgc catgaatgac
      841 aggtcatctg ccaagccatt ccctgccaac gctagcgatt ggagaggacc tcggccagca
      901 gctgaacgcg ggcttcgtca gggcagtgac cggttatgcc caatctctgc tcgggccttg
      961 acgccacaac aacgacggcg ttggctggtg acagtgtgaa ggagacaatg atgcggctta
     1021 attcacgggc aaagccaatg cgcgccgccg tgcctgctgg cagcggtggc agagggcgcg
     1081 gcagcggcac ggaacccagc cgcaactgga ggcggacgtg cgcagcggcg gctgcggccg
     1141 tcgtgcttct ggccggttgt acagcaacgg atcaggaacc gggcacgcag accgagggcg
     1201 cggtctcatc cccggcagca gggcaggctc ctgaacaggg cagcagcgga ggtgtttcca
     1261 taccggacat cgtcgccagc gtggagccct cggtggtgac catctttacc gaaaggggtg
     1321 gtctgggcag cggcgtcatt tacagtgcag acggcttgat cctgaccaac gagcatgtgg
     1381 tccgcggggc ggatgaggtt gaggtgggtt ttgccgatgg caagcgcgag gccggtaccg
     1441 tggtggcgga ggatccggtg acggatcttg ccttggtcca ggtggagcgg acaggcctgc
     1501 ctgccgcgga attcgagccg tcgctgccac gggtggggga gctggccgtg gttatcggca
     1561 gtccgctcgg tttcgcgaac acggcgacgg cgggaatcat ttccgggctg caccgggaga
     1621 ttcccggttc ggcttccaat agccagtcgc tggtggacct gatccagacc gatgccccga
     1681 tcagccctgg caactcgggt ggggcagtgg tcaacagcga gggcaaagtc ataggcatca
     1741 gcgaggccta catcccgccg caggcggggg ccgtagcgct gggattcgcg attccggcgg
     1801 caaatgcaat ggaggttgcc gaacagttgc gcgatgacgg cagcgccgag cacgccttca
     1861 tggggttggg accggcggcc atcacacctc agattgcgga gcagcttgga ttgccggaca
     1921 cgcagggtgc cctggtgctg tcagttgtgg acggcggccc ggccgcggaa gccgggctgg
     1981 agcccggcga tgtgattgtg gggctggggg gcgaggagat tcagacgccc gaggatctgc
     2041 tcgcggcctt gcgcggccat gcgccggggg acgaggtgga agtagagatc agccggggcg
     2101 gtgaaccaag ccagattact gtcacgctcg cggaccgtcc atccgctgaa tgaggcggag
     2161 actcaacagg ccgaatgagg gcagcgcagc agcagaacgg ggcaacgcag cgcagccgga
     2221 atagaacagg ctgctggccg ttatgactgg tgtgccgcta acgaagattc ccgtctccat
     2281 cctggaccgc gccaactccc gctccggcgg aacggaggcg catgccttga gggcgacggc
     2341 catccgcgcg cgccgggcag aggaactcgg ctaccaccgg ttctgggtgg cggaacacca
     2401 tggtgtgccg gggattgcag ggtcggcgcc gaccgtcctg atgggggtca tcgcagcgga
     2461 gacggagcgg atccgcatcg gttccggtgg cgtgatgctg cccaaccacc agccgc
//
LOCUS       DATACA010000311         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1887570, whole genome shotgun sequence.
ACCESSION   DATACA010000311 DATACA010000000
VERSION     DATACA010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887570"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHK28_06365"
     CDS             complement(<1..332)
                     /locus_tag="VHK28_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2141887.1"
                     /translation="MDRERRRFAEAHPRSAALFERSRVSLLGGVPMNWMSKWPGSFPP
                     FVERASGGHFTCVDGHDYVDFCLGDTGAMTGHAPEASVQAIRAQLERGITTMLPTEDA
                     PAVGEELA"
     gene            669..1598
                     /locus_tag="VHK28_06370"
     CDS             669..1598
                     /locus_tag="VHK28_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141888.1"
                     /translation="MVLTLTALAGPAVVSAATPSWVPVSAVALPPAVSPGAEAGYAVT
                     IRNDGPSNISQLYLVTEDATQVPSYTDPSQGSCASSGPLFCSLGALRKNRTVTVVVAF
                     PTPEDGSQLDVTFLWNTTGLGSGGGDSSHGDELRQEVSTALIDHPDFAGAFVRDAGQV
                     HNSQTISSDNPQSTLVNVPATNIAVTVQDGPGVGSANCPANLACFTQTSEIHVANGAT
                     FPGGFSVYIQLDKSQLASGTNANNLDFWHEFDAPRTVGGQIVTGEVIDDACQFRGNSS
                     TPRNMPCKTVSNIGGGDLGATLWLLENGRIQGW"
BASE COUNT          275 a          571 c          536 g          251 t
ORIGIN      
        1 ctggcgagct cctcgccgac ggcgggggca tcctcggtgg gcagcatggt ggtgattccg
       61 cgctccagct gcgcccggat ggcctggacc gatgcctccg gcgcatgccc ggtcatggcc
      121 ccggtgtcgc ccaggcagaa gtcgacgtag tcgtggccgt cgacgcacgt gaagtggccg
      181 ccgctggcgc gctccacgaa cggcgggaac gaaccgggcc acttgctcat ccagttcatg
      241 ggcacgccgc ccaggagtga aacccgcgat cgctcgaaca gagcggccga tcgagggtgc
      301 gcctcggcga agcgtctacg ctcgcggtcc atgagctggc gcaggatcgg acgggctact
      361 gctgtcattc agccgatatt cgcacggtcg ccaggatcac cgggctgatg tcgggcaggt
      421 gactgccgtc aacacatccg acgacccacc cttgtcgggc agcgcgctgg acctgtctgg
      481 agcggtccag tgcggtgtgg gatggtgaaa aagtactagt cacgcaggct ccggacgcct
      541 atcctgtgcg gcacccagct cgacggcagg ttgcgtcccg tgtcccagcg tcggctgcac
      601 cgagcacaga ggtcacttac gcgatgactc gcaggcgatc gatccgttcc ttctcactag
      661 gcctttccat ggttctcacc ctgaccgccc tggccggccc ggccgtggtc tcggccgcta
      721 ctcccagctg ggtcccggtc agcgccgtgg cgctaccgcc ggcagtcagc ccgggggcgg
      781 aggcgggcta cgcggtgacc atcaggaacg atgggccgag caacatctcg cagctgtacc
      841 tcgtcaccga ggacgcgacc caggttccga gctacaccga tcccagccag ggctcgtgcg
      901 ccagcagcgg cccgctgttc tgcagcctgg gcgctctgcg caagaaccgg acggtgacgg
      961 tggtggtggc attccccacg ccggaggacg gctcgcagct cgacgtcacc ttcctgtgga
     1021 acaccaccgg cctcggctcc ggcggcggcg acagcagcca cggcgacgag ttgcggcagg
     1081 aggtgagcac ggcgctcatc gaccatccgg acttcgccgg cgcgttcgtg cgagacgcgg
     1141 gccaggtgca caacagccaa accatctcca gcgacaaccc gcagtccacc ctggtgaacg
     1201 tacccgccac caacatcgcg gtcacggtcc aggacggccc gggcgtgggc agcgccaact
     1261 gcccggcgaa cctggcttgc ttcacgcaga cctccgagat ccacgtggct aatggcgcca
     1321 ccttcccggg cggcttcagc gtctacatcc agctcgacaa gtcccagctg gcatccggca
     1381 cgaacgccaa caacctggac ttctggcacg agttcgatgc acccaggacg gtgggcggcc
     1441 agatcgtcac cggtgaggtg atcgatgacg cctgccagtt ccgcggcaac agcagcacgc
     1501 cgcgcaacat gccgtgcaag acggtctcga acatcggcgg tggtgacctg ggcgccaccc
     1561 tctggctcct ggagaacggg cgcattcagg gctggtagcc tgctcctgaa ccgatcgacc
     1621 gccggcgctc agg
//
LOCUS       DATACA010000312         8420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_923710, whole genome shotgun sequence.
ACCESSION   DATACA010000312 DATACA010000000
VERSION     DATACA010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923710"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VHK28_06375"
     CDS             complement(<1..955)
                     /locus_tag="VHK28_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141889.1"
                     /translation="MPEPATVTQPGRFDWLDRYLRPREGWLALGLLFVMLLSLGWSVQ
                     RAGWLDQADFLIPVAFYAILLGAVLGLSTLSVVATLPIGALVGGGIVLWTVGGEYFPE
                     LSQAGRLLALREEAIDFTRIIVDRGFAPQITPYALGLAAIMWITAFIAAYTLYRHHRV
                     LDAILLVGAFLVANLASTLTDLLGYLVLFMLAALLLWLRAALIGREEGWQRRRVTENT
                     EVPRAIMRSGVAFIAASIAMAWVLTSVAVASPLTAVWNNLDVVWHDVRDQLDGVFGGL
                     SNADSRIGGTVFGSGFNVRGEWFSNDDPVLTYAASQPYYLKT"
     gene            complement(948..2210)
                     /locus_tag="VHK28_06380"
     CDS             complement(948..2210)
                     /locus_tag="VHK28_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2141890.1"
                     /translation="MGSLSRQLRFVFLGTVLVVAAFSTGISFLFFLVYLLGGLLIGAW
                     FYARRGLQGLRAGYHVLNPRAQVGDVLQAVYRVDNDTRWMKPWVELWNDSTLPAPLPG
                     RVIGVQARSSRQWLAKVTLLRRGSYRLGALRVRTGDPFGLFTSEMVVGQPTWVVVFPR
                     VHSLPHWRLPPSPVDGTQPSRRRNESSSPLVSTIRPYVHGDAINRIHWLSSVRHNELQ
                     VKEFDLEQTADLWMVLDLDRSAHAGSGDGASVETAVSAAASIAVQTLGENRAVGLTVS
                     ARRIHILTPDRGQRVEQKILHLMANVQPDGNQPLAEVLMNTLPQLRRGMTLCIVTGST
                     DREWVRALASLRRRGVGTVVVLLDKASFATRPSGEDPAADQRRAELAAVRHALAEYDI
                     GHYFLQAGDDLSEALGGRMPMSRERVRA"
     gene            complement(2233..3195)
                     /locus_tag="VHK28_06385"
     CDS             complement(2233..3195)
                     /locus_tag="VHK28_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2141891.1"
                     /translation="MKNVQELAERVVANVERVIVGKHAEVRMALVALLCEGHILIEDV
                     PGVGKTMLAKAISRSVGCTFRRIQFTPDLLPSDVTGLSIFNQKTQEFEFRPGPIMAQV
                     VLADEINRATPKTQSALLECMEERQATIDGVTHPMPEPFLVIATQNPIEYEGTFALPE
                     AQLDRFMLRLRLGYPRAVEEIVILDEQKRSHPIEELRQVLSYEELRVMQSAVKEVYVD
                     QAVAEYIVRLTTATREHPDVYLGASPRGSINLYRASQALAALDGRDYVIPDDVKALGV
                     AVLAHRLIVKSQASLREVDPDGIVREVLAQVPVGEASSSVVGPR"
     gene            complement(3300..4841)
                     /locus_tag="VHK28_06390"
     CDS             complement(3300..4841)
                     /locus_tag="VHK28_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2141892.1"
                     /translation="MIATNIRRLAVYLLISFAAISAALAWWQVIDAPTLAARQDNPEV
                     IAARRSALRGSIFDAQGRLLASSQVIDGISRRTYVDPAFSHVIGYASLRFGTTGVERA
                     WDDLLTGRADPNPITDLVNDILARRTEPRDLTLTTDKQLQDFAAAQLGSDIGAVVALD
                     PATGAILAMTSTPSFDATPISGDPAAAAAPMEQIQAQPNNPLVARARQGRYTPGSIMK
                     VVTAASALDAGVITPQTTFPDQPREEREGFVVEGFRVLEHDLGNIQPELWPLSPALQV
                     SSNIFFAHVGLELGGEAYLDYARRFGFCDPLSIGTGSRGLPVIASYVTAETDDGDCAP
                     FSDRAELAQASFGQGRVDVTPVQMALVAATIANGGAMPEPFVVRDVRAHAEDPAGGPS
                     ERIVERYDSGEDRVVEEQAAAETRTAMVDAVNAQLGRVFAGGAALGNFGVEGAAAGKT
                     GTAERGPDLPPHSWFIGFAPAQDGATPSIAVAVVVEGGGSGSGRAAPVGGAVMAEWLR
                     IQGGG"
     gene            complement(4838..6208)
                     /locus_tag="VHK28_06395"
     CDS             complement(4838..6208)
                     /locus_tag="VHK28_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2141893.1"
                     /translation="MSARAARSRRFPPVVWRGAELRMLLPLAVLVPLGFAVAHIAQTG
                     TMDPGPMGLAATYVVVMLGAHFALVAAGNRGDPLLLPLVATIGGIGLVMLNRLPQGLR
                     GTSIFGLQLGMAETQVLWFSVGVVAMLVVAIGFRDDGILRHYKYTWAIAGALLLAVTF
                     LFGVEQFGARLWLNLGPVQVQPGEAIKIVLVVFIAGYLAEHRTLLAGASLRIGPIRIP
                     PLPYLLPMLAIFVIVMLIVVVMRDLGTALLFLGIFLTMLFVATGRRSYVALGILLFVA
                     GSYVAYNLFGHVQVRINNWIDPFADPSGDGYQTVQALYAFARGGLFGEGLGQGLPTIG
                     GNLPIPALETDFIFAAVAEELGLIGAFALLALAAALVFRGLRIAMLARDDFSSMLAVG
                     LTTSLGLQTLIIAAGNVKLIPLTGITFPFVSYGGSSILASFVVVGLLLSVSHRSAAAA
                     AEGRGP"
     gene            complement(6198..6641)
                     /locus_tag="VHK28_06400"
     CDS             complement(6198..6641)
                     /locus_tag="VHK28_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX2141894.1"
                     /translation="MTGEVLRILLLVLQLGFLAILYLILLGFARSLLRDLRSAEQAQL
                     ASQAGIGRLMVVESPENEPPAGRTIALGPINSIGRNVNNTIFVEDDFVSANHAMLTFR
                     GRNWFIEDQGSTNGTYVNGHRIDRPVALSFGDELTIGRVRMRLER"
     gene            complement(6638..7396)
                     /locus_tag="VHK28_06405"
     CDS             complement(6638..7396)
                     /locus_tag="VHK28_06405"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEX2141895.1"
                     /translation="MGILSRLESILQRVFESPAGRLGASLQPVSLAKRIERAMDTTKT
                     FGDDGVIVPNQYVLHLNPADYAGFESYRGSLEDDLAHGVLARARRERYTLVARPRVRI
                     VADNVIPRGDIRVAATVVDEEGARIRADSPLPASSDTMVFARPGHEAASPDSAKRAYL
                     LVRTDGAPAVQFDLGGALISVGRASDNDVIVDDPLVSRHHCQLKLQHGAYSFADLGSR
                     NGSFVNGQPVNEVALGPGDIIRIGSTEIEFQVRG"
     gene            7781..>8420
                     /locus_tag="VHK28_06410"
     CDS             7781..>8420
                     /locus_tag="VHK28_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141896.1"
                     /translation="MPEALTESFCERCGTRYEFAAPTRLNPLRKTRGVISGLKNYIMS
                     QDALSDAVGDAIRSEEEVLAAQQLEAFHSSFNFCINCRQYTCTNCWNDGEGRCRTCAP
                     VAGTDDLMERFEASFQAQHPELLAVAPETELDEVELNRRLGVETWPSEDLSRVEGNGH
                     ADVEWPAEEILSHRPDVAFEPEPEPEPMVAEVEPEPMVAEVEPEPTVAEVEPE"
BASE COUNT         1356 a         2899 c         2946 g         1219 t
ORIGIN      
        1 ccgtcttgag gtagtacggc tggctggcgg cgtaggtgag caccgggtcg tcgttgctga
       61 accactcccc gcggacgttg aagccggagc cgaagaccgt gccgccgatc cgcgagtccg
      121 cgttggacag gccgccgaag acgccgtcca gctggtcgcg aacgtcgtgc cacaccacat
      181 ccaggttgtt ccagaccgcc gtcagcggcg aagccaccgc gacgctggtc agcacccagg
      241 ccatggcgat gctggcggcg atgaacgcca cgccgctgcg catgatggcc cgcggcacct
      301 cggtgttctc ggtcactcgg cggcgctgcc agccctcctc ccgaccgatg agcgcggcgc
      361 gcagccagag caggagggct gccagcatga agagcaccag gtagccgagg agatcggtca
      421 gggtgctggc caggttggcg accaggaacg cgccgaccag caggatggcg tccaggaccc
      481 gatggtggcg gtacagcgtg taggccgcga tgaaggcggt gatccacatg atcgcggcca
      541 gcccgagcgc gtacggcgtg atctggggcg cgaatccgcg atcgacgatg atgcgggtga
      601 agtcgatcgc ctcctcccgc agggcgagca gccggcccgc ctgggacagc tccgggaagt
      661 actcgccgcc gacggtccat agcacgatgc ccccgccgac gagcgccccg atcggcagcg
      721 tggcgaccac gctcagcgtg ctgagcccca gcacggcccc gagcaggatg gcgtagaagg
      781 cgaccgggat caggaaatcg gcctgatcca gccagccggc gcgctgcacg gaccagccga
      841 ggctgaggag catgacgaac agcaggccca gcgccagcca cccctcgcgg ggccggaggt
      901 atcggtccag ccagtcgaac cgacccggtt gggtgacggt ggccggctca ggcacggacg
      961 cgctcccggg acatgggcat ccggccgccg agcgcctcgg acaggtcgtc gccggcctgc
     1021 aggaagtagt ggccgatgtc gtactccgcc agcgcgtggc gcacggccgc cagctccgcg
     1081 cgccgctggt cggcggcggg gtcctcgccg gacggccggg tggcgaacga ggccttgtcc
     1141 agcagcacga ccacggtgcc cacgccgcga cgccgcaggg aggccagggc gcggacccat
     1201 tcccggtccg ttgagccggt cacgatgcac agcgtcatgc cgcgccgcag ctgcggcagc
     1261 gtgttcatca acacttccgc cagcggctgg ttgccgtccg gctgcacgtt ggccatcagg
     1321 tgcaggatct tctgctccac ccgctgcccg cggtccggcg tgaggatatg gatgcggcgc
     1381 gccgagacgg tgaggccgac ggcacggttc tctcccagtg tctgtacggc gatagacgcc
     1441 gccgcgctca ccgcggtttc aaccgacgcg ccgtcgccgc tgccggcgtg cgcgctgcgg
     1501 tccaggtcga ggaccatcca caggtcggcg gtctgctcca ggtcgaactc cttcacctgc
     1561 agctcgttgt ggcgcacgct ggacagccag tggatgcggt tgatggcgtc gccgtggacg
     1621 tacggccgga tggtgctcac cagcgggctg ctcgactcgt tgcggcgccg cgacggctgg
     1681 gtgccatcca ccggggaggg cggcagccgc cagtgcggca gtgagtggac gcgggggaag
     1741 acgactaccc aggtcggctg gccgaccacc atttcgctgg tgaacagccc gaaggggtcg
     1801 ccggtccgca cccgcagggc accgaggcgg tacgaccctc ggcgcagcag ggtcaccttg
     1861 gccagccact ggcggctgct gcgggcctgc accccgataa cgcgcccggg cagcggcgct
     1921 ggcagcgtgg agtcgttcca cagctcgacc catggcttca tccagcgcgt gtcgttgtcc
     1981 acccggtaga cggcctgcag cacgtcgccg acctgcgccc tggggttcag gacgtggtag
     2041 ccggcgcgca ggccctggag cccccgacgc gcgtagaacc aggcgccgat gagcaggccg
     2101 ccgagcaggt agaccaggaa gaaaagaaag ctgatgcccg tgctgaaggc ggcgacgacc
     2161 aggacggtcc ccagaaagac gaaccgcagc tgacggctca ggctgcccac cggatctcct
     2221 ccccgatcag cgtcagcgcg ggccgacgac gctgctgctc gcctcgccga cgggcacctg
     2281 cgccagcacc tcgcgcacga tcccgtccgg atcgacctct cgcagggacg cctggctctt
     2341 gacgatcagc cgatgggcca gcacggccac ccccagcgcc ttcacatcat cggggatgac
     2401 gtagtcgcgc ccgtccagcg cagcgagcgc ctggctggcg cggtacaggt tgatggagcc
     2461 gcgcggggag gcacccaggt acacgtccgg gtgctcgcgg gtggccgtgg tcaggcgcac
     2521 gatgtactcg gcaaccgcct gatcgacgta gacctccttg actgcggact gcatgacgcg
     2581 cagctcttcg taggagagga cctggcgcag ctcctcgatc gggtggctgc gcttctgctc
     2641 atcgaggatc acgatctcct cgacggcgcg cgggtacccc aggcgcaaac ggagcatgaa
     2701 gcggtcgagc tgggcctccg gcagggcgaa ggtgccctcg tactcgatcg ggttctgggt
     2761 ggcgatcacc aggaatggtt cgggcatggg gtgcgtcacg ccgtcgatgg tcgcctggcg
     2821 ttcctccatg cattcgagca gtgcgctctg ggtcttcgga gtggcgcggt tgatctcatc
     2881 ggccaggaca acctgggcca tgatcggtcc cggccggaac tcgaactcct gcgtcttctg
     2941 gttgaagatc gacaggccgg tcacgtccga tggcagcagg tccggcgtga actggatgcg
     3001 gcggaacgta caccccacgc tccggctgat ggccttggcc agcatcgtct tgcctacgcc
     3061 cgggacgtcc tcgatgagga tgtgtccctc gcacagcagc gcgacgaggg ccatccgcac
     3121 ctcggcgtgc ttgccgacga tcacgcgctc gacgttggcg acgacgcgct ccgccagctc
     3181 ctggacgttc ttcacctatg gcaccccttc ggtctgggcg gacgctcggt ggcctccctc
     3241 gcgtttcaca ataccccacc gggttacgaa ccggttcatc cccgctcgaa cgctgtcgct
     3301 cagccgccgc cctggatgcg cagccactcg gccatgaccg cgccgccaac aggggccgcc
     3361 cggccggatc cgctgccgcc gccctccacc accaccgcca ccgcgatcga cggcgtggcc
     3421 ccgtcctgcg cgggggcgaa gccgatgaac cacgagtgtg gcggcaggtc aggtccgcgc
     3481 tcggccgtgc ccgttttgcc ggcggccgcg ccctccacac cgaaattacc cagcgcggcg
     3541 ccaccggcga agacgcgacc cagctgggcg ttgaccgcgt cgaccatggc cgtgcgggtt
     3601 tcggccgcag cctgctcctc caccacccgg tcctcgccgc tgtcgtagcg ctcgacgatc
     3661 cgctcgctgg gcccgcctgc cggatcctcc gcgtgcgcgc ggacgtcacg caccacgaac
     3721 ggctcgggca tcgccccgcc gttggcgatg gtggccgcca ccagcgccat ctgcaccgga
     3781 gtgacgtcca cgcgtccctg cccgaaggac gcctgcgcca gctccgcccg gtcgctgaag
     3841 ggtgcgcagt caccgtcatc ggtctcggcg gtgacgtagg aggcgatcac cggcaggcca
     3901 cgcgagccgg tgccgatgct gagcggatcg cagaagccga agcggcgggc gtagtccagg
     3961 tacgcctcgc cccccagctc caggccgacg tgggcgaaga agatgttgct cgagacctgg
     4021 agcgccggcg agagcggcca cagctcgggc tggatgttgc cgaggtcatg ctccagcacg
     4081 cggaaaccct cgaccacgaa cccctcgcgc tcctcccgcg gctgatcggg gaaggtcgtc
     4141 tgcggggtaa tgacgccggc gtcgagcgct gaggcggcgg tgaccacctt catgatcgag
     4201 cccggcgtgt agcggccctg gcgagcgcgg gccaccagcg gattgttggg ctgggcctgg
     4261 atctgctcca tcggcgcggc ggcggccgcg gggtcgccgg agatcggggt ggcatcgaag
     4321 ctcggggtcg acgtcatggc caggatcgcc ccggtggccg ggtcgagggc gaccaccgcc
     4381 ccgatgtccg agccgagctg ggcggcggcg aagtcctgta gctgcttgtc ggtggtgagg
     4441 gtcaggtcgc gcggctccgt cctgcgggcc aggatgtcgt tcaccaggtc ggtgatcggg
     4501 ttcggatccg cgcgaccggt cagcaggtcg tcccaggcgc gctccacccc ggtggtgccg
     4561 aagcggaggc tggcgtagcc gatgacgtgg ctgaaggccg gatccacgta cgtgcggcgg
     4621 ctgatgccat cgatcacctg gctggaggcc agcagccggc cttgcgcgtc gaagatcgac
     4681 ccgcgcagcg cgctgcgacg agcggcgatc acctccggat tgtcctggcg cgcggccagc
     4741 gtcggcgcgt cgatcacctg ccaccaggcc agcgcggcgc tgatggcggc gaaggagatc
     4801 agcaggtata ccgccaggcg gcgaatgttg gtggcgatca tggcccgcgg ccctcggcag
     4861 cagcggcggc ggaccgatgg ctgaccgaga gcagcaaacc gaccacgacg aagctggcca
     4921 ggatgctcga tccgccgtag ctgacgaacg ggaaggtgat gccggtgagc ggaatgagct
     4981 tcacgttccc ggcggcgatg atcagggtct gcagccccag gctggtggtc agtcccacgg
     5041 cgagcatgga gctgaagtcg tcgcgcgcca gcatggcgat ccgcaggccg cggaagacga
     5101 gcgcagcggc cagggccagc agggcgaagg cgccgataag ccccagctcc tcggccaccg
     5161 cggcgaagat gaagtcagtt tccagggccg ggatgggcag gttgcccccg atggtcggca
     5221 gcccctgccc cagcccctcg ccgaacagcc cgccgcgggc gaaggcgtac agcgcctgga
     5281 cggtctggta gccgtccccg ctcggatcgg caaagggatc gatccagttg ttgatgcgca
     5341 cctggacgtg tccgaacagg ttgtaggcca cgtacgagcc ggccacgaag aggaggatgc
     5401 ccagcgccac gtagctgcgg cggccggtgg ccacgaacag catggtcagg aagatgccca
     5461 ggaacagcag cgcggtgccc aggtcacgca tgaccaccac gatcagcatc acgatgacga
     5521 agatggcgag catcggcagc aggtagggca acggcgggat ccgtatcggt ccaatccgca
     5581 ggctggcacc cgccagcagg gttcggtgct cggccaggta gccggcaatg aacaccacca
     5641 gcacgatctt gatcgcctcg ccgggctgca cctggaccgg ccccaggttg agccacagcc
     5701 gggcaccaaa ctgctcgaca ccgaacagga aggtgacggc cagcagcagc gccccggcga
     5761 ttgcccaggt gtacttgtag tgccgcagga tgccgtcgtc gcggaagccg atggccacca
     5821 ccagcatggc caccaccccc acgctgaacc acagcacctg ggtctcggcc atgccaagct
     5881 gcagcccgaa gatgctggtg ccgcgcagcc cctggggcag gcgattgagc atgaccagac
     5941 cgataccacc gatcgtcgcc accagtggca gcagcagcgg gtcgcctcgg ttgcccgcgg
     6001 ccaccagggc gaagtgggca cccagcatga ccaccacgta cgtggccgcc aggcccatcg
     6061 gtcccggatc catggtcccc gtctgggcga tgtgggcgac cgcgaagccc agcggcacga
     6121 gcaccgccag cggcagcagc atccgcagct ccgccccgcg ccagacgacc ggcgggaagc
     6181 gccggctgcg ggccgcccta gcgctcaagg cgcatccgca cgcggccgat ggtcagctcg
     6241 tccccgaagg agagcgccac cggacggtcg atgcggtggc cgttgacgta cgtgccgttt
     6301 gtgctgccct ggtcctcgat gaaccagttc cggccgcgga aggtgagcat ggcgtggttg
     6361 gcgctgacga agtcgtcctc cacgaagatg gtgttgttca cgttgcgccc gatcgagttg
     6421 atgggcccca gcgcgatggt ccggccggcc ggaggctcgt tctccggcga ctccaccacc
     6481 atcaggcgcc cgatgcccgc ctggctggcc agctgcgcct gctccgcgct gcgcagatcg
     6541 cgcagtagcg agcgcgcgaa gccgagcagg atcaggtaca ggatggccag gaatcccagc
     6601 tgcaggacca gcagcaggat gcgcagcacc tcgccggtca tccccgcacc tggaactcga
     6661 tctccgtgga gccgatgcgg atgatgtcgc ccgggcccag cgccacttcg ttcaccggct
     6721 gaccgttgac gaaggagccg ttccggctgc cgaggtcggc gaagctgtag gccccgtgct
     6781 ggagcttgag ctggcagtgg tgccggctga ctagcgggtc gtccacgatg acgtcgttgt
     6841 cgctcgcccg ccccacgctg atgagggcgc cgccgaggtc gaactgcacg gccggggcgc
     6901 catcggtccg caccaggagg taggcgcgct tcgccgagtc cgggctggcc gcctcgtgac
     6961 cgggcctggc gaagaccatg gtgtccgagg atgcgggcag cggcgagtcc gcccggatgc
     7021 gggcgccttc ctcgtccacc acggtggcgg ccacccggat gtcgccgcgt ggtatgacat
     7081 tgtcggccac gatgcgcacc cgtggacggg ccaccagggt gtagcgctcg cgccgcgcgc
     7141 gggcgagcac cccgtgcgcc aggtcgtcct ccagcgagcc gcggtacgac tcgaagccgg
     7201 cgtagtcggc cggattgagg tgcaggacgt actggttggg cacgatcacg ccgtcgtcgc
     7261 cgaacgtctt ggtggtgtcc atggcccgct cgatgcgctt tgccagcgac accggctgga
     7321 gtgatgcccc aaggcgtccg gccggtgact cgaacacgcg ctgcaggatg gattccaagc
     7381 gtgaaaggat gcccaccggc cgatgctagc acgggtgtcc ggcacccctt tgagcgtgaa
     7441 tcccgagcgt ccggctggcg ttccgttggc cggtactgtc gcgtcatcgc gacggggccg
     7501 aattcacacc cggtgaccag cccgctcggt tttgtcccga tttcgcggtc cgaattcacg
     7561 ccaccggtgc attcgtacgc ttccgcgggc cccgtcaacc cacaacgccc cacctggtgt
     7621 gaatctggac gattcgcggc ggcctcatgg cgctctttac atgatgcgca ggtaccacct
     7681 gcgcttcggc ggagtaccaa agccccggta gggtcccatt gtggggcgtg gctatactcg
     7741 tgctccctcc gctgccctcc aggggaaacg cgatatcgac atgcccgaag cgctcaccga
     7801 atccttctgc gaacgatgcg gaactcgcta cgagttcgcc gctccgacgc gcctgaatcc
     7861 tctccgcaag acgcgcgggg tgatcagcgg gctcaagaac tacatcatga gccaggatgc
     7921 cctgagcgat gcggtgggcg acgccatccg gtccgaggag gaggtcctcg ccgctcagca
     7981 gctggaggcc ttccacagct cgttcaactt ctgcatcaac tgccgccagt acacctgcac
     8041 caactgctgg aacgatggtg aggggcggtg ccgcacgtgc gccccggtcg ccggcacgga
     8101 tgacctcatg gagcgcttcg aggccagctt ccaggcgcag caccccgagc tcctcgccgt
     8161 ggcaccagag accgagctgg acgaggtcga gctgaatcgc cggctcggcg tggagacatg
     8221 gcccagcgag gatctgtcgc gcgtggaggg caacggccat gccgacgtcg agtggccggc
     8281 cgaggagatc ctttctcatc ggccggacgt ggcgttcgag cccgagccgg agccggagcc
     8341 gatggtggcc gaggtggagc ccgagccgat ggtggccgag gtggagccgg agccaacggt
     8401 ggccgaggtg gagccggagc
//
LOCUS       DATACA010000313         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_120035, whole genome shotgun sequence.
ACCESSION   DATACA010000313 DATACA010000000
VERSION     DATACA010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120035"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VHK28_06415"
     CDS             <1..323
                     /locus_tag="VHK28_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX2141897.1"
                     /translation="TLIKAEFDRVLTDVDALLAPTSPSVAFKIGAKTEDPLLMYLNDA
                     CTLPVNIAGLPGVSVPCGLSDGLPVGLQVIGRAFDESTVLRVADAYERAAGFAALRPP
                     TAHD"
     gene            581..946
                     /locus_tag="VHK28_06420"
     CDS             581..946
                     /locus_tag="VHK28_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2141898.1"
                     /translation="MAARLAASDIGRARAWYEEKLGLVPEKDEMGGVSLWYRTADTWF
                     LLYQTDAAGTARNTVGGWEVENLDAVMAELRKRGVTFEDYDFGEMKTVDGVLSMGGYR
                     AAWFRDSEGNILELTESPS"
     gene            1013..1441
                     /locus_tag="VHK28_06425"
     CDS             1013..1441
                     /locus_tag="VHK28_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX2141899.1"
                     /translation="MRIGEVAARLGVSTDTIRFYERSGLLPRPRREENGYREYSQPEI
                     ERLRLLLDLRRLDLPLEDAARLAGWCQSGHCQETTQELPALIAARREEISRRIDDLRL
                     LDSRLAALGRHLELSSLPVLGAEPCCPAAAALADLGATTA"
     gene            1492..2073
                     /locus_tag="VHK28_06430"
     CDS             1492..2073
                     /locus_tag="VHK28_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141900.1"
                     /translation="MAEISHVRMAVPEQGPLNRILREGGAQAFPQWLYAEPGDEPRIL
                     WWGVRGAPTAALTAPEDPARASLLPRPIDDWTESPRALILATVDADRAAADLQPAIGA
                     GWREVGEDAVLGARCRIKEIGRSELVLAEPSTEGYVAACLARFGEGPVAVALDGTTAA
                     GREVTRNPVSGGPATWVRIGPGTAPYLLFLPAR"
     gene            2070..>2506
                     /locus_tag="VHK28_06435"
     CDS             2070..>2506
                     /locus_tag="VHK28_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX2141901.1"
                     /translation="MTTAEHRAAVEAWWTARDERLRDPDGWLTLVGLHWLTPGDNRFG
                     TDASNEIVLRGQDVPAHAGTIRLEGDTPWLLGPDGSRALEAPLADDTSGEPTVLDVGS
                     LRMCVIRRGERLGLRVRDHDSAALTTFSGMDRFPFDPSWRVAGG"
BASE COUNT          352 a          855 c          933 g          366 t
ORIGIN      
        1 ggacgctgat caaagcggag ttcgaccggg tgctgaccga tgtggacgcc ctgctggccc
       61 caacctcgcc cagcgtcgcg ttcaagatcg gcgccaagac cgaggacccg ctgctgatgt
      121 acctcaacga tgcctgcacc ctcccggtca acatcgccgg gctgccggga gtatcggtcc
      181 cgtgcggcct gtccgatggc ctgccggtcg gattgcaggt gatcggtcgc gcctttgatg
      241 agtcgaccgt cctgcgggtg gccgatgcct acgagcgcgc ggccggcttc gctgccctcc
      301 gtccgcccac tgcccacgac tgacccttac ggtcggctgt gcggctgtgc ggctgtgcag
      361 cacaaacggg gtctgacagc gtgacacccc ctccgccctg acccccccgc gcccgtcagg
      421 ggtcgattgt gcacctgtgc ggctgtgcgg ctgtgccgca caaacggggt ctgacagcgc
      481 gagacccggg taccccttga cattggagtc gctccaggtc gtagcgttac gtcacgcaca
      541 caactcacac aggagaacag ggatgctggg tgactttccg atggcggccc ggctcgcggc
      601 gtcggacatc ggtcgggcga gagcctggta cgaggagaag ctcggcctgg tcccggagaa
      661 ggatgagatg ggcggcgtct cgctgtggta caggacggcg gacacctggt tcctgctgta
      721 ccagaccgat gccgccggca ccgcccgcaa cacggtcggc ggttgggagg tcgagaacct
      781 ggacgccgtc atggccgagc tgcgaaagcg cggcgtcacg ttcgaggact acgacttcgg
      841 ggagatgaag acggtggacg gcgtgctgtc gatgggcggc taccgcgccg cctggttccg
      901 ggacagcgag gggaacatcc tggagctgac cgagagtccg agctgagccc ggcgcaatgc
      961 gcccgtagcg gaggatcttg acaggcgggg aacgacggcc gacgctcccg ccatgcgcat
     1021 cggcgaggtg gcggccaggc tcggcgtctc cacggacacc atccgcttct acgagcggag
     1081 tgggctgctg ccgcggccgc ggcgcgagga gaacggctac cgggagtact cccagccgga
     1141 gatcgagcgc ctgcgcctcc tcttggactt gaggcggctg gaccttccgc tggaggacgc
     1201 ggcgcgcctg gccggctggt gccagagcgg gcactgccag gagacgacgc aggagctgcc
     1261 ggcgttgatc gcggcacggc gcgaggagat cagccgccgc atcgatgacc tgcggctgct
     1321 ggacagccgg ctcgccgcgc tcggtcgtca tttggagctg agcagcctgc cagtgctggg
     1381 cgcagagccc tgctgcccgg ccgcggcggc gctggcggac ctcggcgcca ccaccgcctg
     1441 atcctgccgc tcccccgcga gtggccggcg ccagcggcta gactcgcggc catggccgag
     1501 atcagccacg tgcgcatggc cgtgccggag caggggccgc tcaaccggat cctgcgcgag
     1561 ggcggggcgc aggcctttcc gcagtggctc tacgccgagc cgggcgacga gccgcggatc
     1621 ctgtggtggg gcgtccgcgg cgcaccaacc gctgcactca cggcgcccga ggatccggcg
     1681 agggcctcgc tcctcccgcg accgatcgac gactggaccg agtcgccgcg cgccctgatc
     1741 cttgccaccg tggatgccga tcgggccgcg gccgacctgc agccggccat cggcgccggg
     1801 tggcgcgagg tgggggagga cgccgtgctc ggcgcgcgct gccggatcaa ggagatcggc
     1861 cgcagcgagc tggtgctcgc cgagcccagc acggagggct acgtggctgc ctgcctggcc
     1921 cgcttcgggg agggcccggt ggcggtggcg ctggatggga ccactgcggc cggccgggag
     1981 gtcacccgta acccggtgag tggcgggccg gcgacctggg tgcgcatcgg tcccggcacc
     2041 gcgccgtacc tgctcttcct ccctgcccga tgacgacggc cgagcaccgt gccgcggtcg
     2101 aagcgtggtg gacggcgcgc gacgagcggc tgcgcgaccc ggacggatgg ctgacgctgg
     2161 tcgggctgca ctggctgacc cccggggaca accgcttcgg gaccgatgcg tccaacgaga
     2221 tcgtgctgcg cggccaggat gttcccgccc atgccggaac gatccggctg gagggggaca
     2281 ccccgtggct gctgggaccc gatggctccc gcgccctgga agcacctctg gccgatgaca
     2341 cgagcggtga gcccacggtg ctcgacgtgg gctcgctgcg catgtgtgtg atccgccgtg
     2401 gggagcggct tggcctgcgg gtgcgcgacc acgactcggc ggccctgacc acgttcagtg
     2461 gcatggatcg ctttccgttc gacccgtcgt ggcgcgtggc cggggg
//
LOCUS       DATACA010000314         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1003788, whole genome shotgun sequence.
ACCESSION   DATACA010000314 DATACA010000000
VERSION     DATACA010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003788"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VHK28_06440"
     CDS             complement(<1..461)
                     /locus_tag="VHK28_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141902.1"
                     /translation="MSTYSQDGFTPDPGTPVYEDISLDATSTSGTSTQSGGKTDAAKQ
                     EAAGVAQDAKQAGKQVAGTAVDQSKQVAGTAKEQARRVTSEAATQVRGLVGQAQTELR
                     QQASAQQDRVAQGIRTLGEQLNSMAAGNMPEEGPALNIVRSVADRADAAAS"
     gene            986..>1939
                     /locus_tag="VHK28_06445"
     CDS             986..>1939
                     /locus_tag="VHK28_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141903.1"
                     /translation="MSIARIYPYARPAIPRIALGMVAALIGALVALAIPQVLRSLVDG
                     PLRDGDGSAVWATGALVLGLGVLEAAMVALRRWFVLVPNTVVELRMRTDLYAKLQDLS
                     VSFHDRWPSGQLLSRIVSDLNLVRRWLSFGLVLFVVNIVTIGIGFTVLISINWMLGVL
                     FAVCSIPLWIFGYFFERKYSVVARRSQDQAGDLATTVEQSVHGIRVLKAFGRGKHALR
                     NFASQADELRNTELEKARAIAGIWLWLLLVPDVTFALALFGGVVLAADGQVSVGALVA
                     FFATATVLRFPVESIGFLLAMTFDTRTAIDRFFEVMDEPVTI"
BASE COUNT          240 a          691 c          659 g          349 t
ORIGIN      
        1 cagctggctg cggcgtcggc gcggtcggcg accgagcgga cgatgttgag cgctgggccc
       61 tcctcgggca tgttcccggc cgccatcgag ttcagctgct cgccgagcgt gcggatgccc
      121 tgtgcgacgc ggtcctgctg agcgctcgcc tgctggcgga gctcggtctg cgcctggccg
      181 accagcccgc gcacctgcgt ggcagcctcc gaggtcaccc tcctggcctg ctccttcgcg
      241 gtgccggcga cctgcttgct ctggtccacg gcggtgccgg cgacctgctt gcccgcctgc
      301 ttggcgtcct gggcgactcc cgccgcctcc tgcttcgcgg cgtcggtctt gccgccgctc
      361 tgggtggacg tgccgctggt gctggtcgcg tccagcgaga tgtcctcgta gacgggggtg
      421 ccggggtccg gtgtgaaccc atcttgtgaa tacgtgctca ttgcatccct tcctgacgtg
      481 tcttccacac gtgtgtgccc gcgctgggga acgcggaggt gcggctttcc accaccttgc
      541 ggcccgcgcc tgaaaaccag accatccgcg aaggcatcct cagcaacctg ccgaccgccg
      601 tccaggaggc gttcagaaac aggattccgg gatgaggttg ttgcgtcacg acagatcgcc
      661 cgcataggct ggagggctcc ggccgcgaca ggccggtcgg agcttcggga gacacgggag
      721 gccgtcatgc gattcgagac gatccagcgc aagatgtcct tcacggccgt cgggatccac
      781 cccgccctcc gcctccggac cccttccgcc gcctagcggc acccggtccg ggcgcaccgc
      841 ccgccagcac cacacccgcc ctgaagcccc gccgggcttc gggcacgaat caccccgagc
      901 ccgccgacgt ccgtcgggcc gatcaccgga aacacatatg cctgcgcgtt ctgagccctc
      961 tggccgtccg accctgtcca ccttcatgtc gatcgcccgc atctacccgt acgcgcggcc
     1021 ggcgatcccg cgcatcgcgc tcggcatggt cgcggcgctg atcggcgcgc tcgtcgcgct
     1081 cgccatcccg caggtcctgc ggtcgctcgt cgacgggccg ctgcgggacg gcgacggcag
     1141 cgcggtctgg gcgaccgggg cgctggtgct cggcctcggt gtgctcgagg cggcgatggt
     1201 ggcgctgcgt cgctggttcg tcctggtgcc gaacacggtc gtcgagctgc ggatgcgcac
     1261 cgacctgtac gccaagctgc aggacctgtc ggtctccttc catgaccgct ggccgagcgg
     1321 gcagctgctg tcccgcatcg tgagcgacct caacctggtg cggcgctggc tgagcttcgg
     1381 cctggtgctg ttcgtcgtga acatcgtcac catcggcatc gggttcaccg tgctgatcag
     1441 catcaactgg atgctgggtg tgctgttcgc ggtgtgctcc atcccgctgt ggatcttcgg
     1501 ctacttcttc gagcggaagt actcggtggt cgcgcggcgc agccaggatc aggccggcga
     1561 cctcgccacg accgtggagc agtcggtgca cggcatccgg gtgctcaagg cgttcggccg
     1621 cggcaagcac gcgctgcgga acttcgcgtc gcaggcggac gagctgcgga acacggaact
     1681 ggagaaggca cgggccatcg ccgggatctg gctgtggctg ctgctcgtgc ccgacgtcac
     1741 cttcgcactg gcgctgttcg gcggcgtggt gctcgccgcg gacgggcagg tgagcgtcgg
     1801 cgcgctggtc gcgttcttcg ccaccgccac cgtgctccgc ttcccggtcg agtccatcgg
     1861 gttcctgctg gcgatgacct tcgacacccg aaccgcgatc gaccgcttct tcgaggtcat
     1921 ggacgagccg gtgacgatc
//
LOCUS       DATACA010000315         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1163936, whole genome shotgun sequence.
ACCESSION   DATACA010000315 DATACA010000000
VERSION     DATACA010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163936"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VHK28_06450"
     CDS             complement(<1..722)
                     /locus_tag="VHK28_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX2141904.1"
                     /translation="MRKDTPPFRRLLVANRGEIAIRIMRACRELGIESVAVYSDADAE
                     ALHVEFADRAERIGPAPAAESYLSITALLDAARRSGAEAVHPGYGFLSENDTFASAVE
                     DAGLVWIGPPPATQAALGNKLAARRAVSEAGVPVVPGLLVPLDGQGEIGLAEVGFPAM
                     LKAAAGGGGRGMRRLDGPADLATGLSSARREAQAAFGDGTLYLERLISPARHIEVQLL
                     GDHHGRLAVLGERECSVQRRHQ"
     gene            complement(734..2284)
                     /locus_tag="VHK28_06455"
     CDS             complement(734..2284)
                     /locus_tag="VHK28_06455"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2141905.1"
                     /translation="MSTAEKLERLRRMREQAMQGGGERRIAQQHERGKLTARERLELL
                     LDPESFVELDAFVTHRATDFGIGQEQYLGDGVVTGHGLIEGRTVFVYAQDFTVFGGSL
                     SEAHAEKIVKVMDLAMENGAPIIGLSDSGGARIQEGVVSLGGYAEIFLRNTLASGVVP
                     QLSLVLGPCAGGAVYSPAITDFTIMVDGTSYMFVTGPNVVKTVTHEQIDFEGLGGARV
                     HNETSGVAHFLSPGEPQALDLARRLVGYWPSNNVDPAPRMEEWLPPAMSPEDLDAVIP
                     DSPSQAYDMHRVIGGIVDGGSFTEVQAHFARNVICGFSRIEGRSVGIVAQQPEVLAGV
                     LDIDSADKAARFVRFCDCFNIPLVTLVDVPGFLPGVDQEHRGIIRHGAKLLYAYSEAT
                     VPKVTVITRKAYGGAYDVMSSKHVRGDFNFAWPTAEIAVMGVEGAVNIIFRERISASD
                     DPDAERSRLVAEYEERFANPYVAAARGYVDAVILPSETRARLAAALTVLEGKRQSVPP
                     KKHGNIPL"
     gene            complement(2302..2377)
                     /locus_tag="VHK28_06460"
     tRNA            complement(2302..2377)
                     /locus_tag="VHK28_06460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2342..2344),aa:Ile,seq:cat)
     gene            2560..>3278
                     /locus_tag="VHK28_06465"
     CDS             2560..>3278
                     /locus_tag="VHK28_06465"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141906.1"
                     /translation="MTKVISIANQKGGVGKTTTSINLAGALAELGYRVLCIDMDPQAN
                     LTVGLGISLSDVRYSMGDVLADNRVPLDEVVRQTEMPGLSVAPATLELASTEVELFTA
                     IGREMVLREALTGWAERQFDVVIIDCPPTLGLLTINSLVASNRVIIPVQTQYYAIKGL
                     TALIKVINTIKLKLNHDLEILGLLATFFDGRTVLAREMLQNLREIGDHRVFNTMIKNT
                     VKLGEAPLTGKPVTEYATNSAA"
BASE COUNT          542 a         1149 c         1068 g          519 t
ORIGIN      
        1 ttctggtggc ggcgctggac gctgcactcc cgctcgccga ggaccgccaa ccggccgtgg
       61 tggtcgccga gcagctggac ctcgatatgc cgcgccgggc tgatcagccg ctccaggtag
      121 agggtgccgt cgccgaatgc cgcctgcgcc tcgcgtcgcg cggaggacag cccggtggcc
      181 aggtctgcag gcccgtccag gcgccgcatg ccacggcccc cgccacccgc tgccgccttg
      241 agcatggccg ggaagccgac ctccgccaag ccgatctccc cctgcccatc cagcgggacc
      301 aggaggccgg ggacgaccgg aaccccggcc tcgctgaccg cccgtctggc ggccagcttg
      361 ttccccagtg cggcctgggt ggccggcggc ggaccgatcc acacgagtcc ggcatcctcc
      421 accgcgctgg cgaaggtgtc gttctccgac aggaacccgt atcccggatg gaccgcctcg
      481 gccccgctgc gacgtgccgc atccagcagg gcggtgatgc tcagatagga ctcggccgcc
      541 ggcgcgggac cgatgcgctc ggcccgatcc gcgaactcga catgcaatgc ttcggcgtcc
      601 gcatcggagt agacggccac gctctcgatg cccagctcgc ggcaggccct catgatgcgg
      661 atcgcgatct cgccgcggtt ggccaccagc agccggcgga agggcggggt gtctttgcgc
      721 acggacaccg atgctagagc gggatgttgc cgtgcttctt gggtggcacc gattggcgct
      781 ttccctccag cacggtcagg gccgccgcga gccgagcacg cgtctccgac ggcaggatga
      841 cggcatcgac gtacccgcgg gccgcggcca cgtaggggtt ggcaaagcgc tcctcgtact
      901 cggccaccag gcgcgatcgc tccgcgtccg ggtcgtctga cgccgagatt cgttcgcgga
      961 agatgatgtt gaccgcgccc tcgacgccca tcactgcgat ctcggccgtc ggccacgcga
     1021 agttgaagtc gccgcggacg tgcttgctgc tcatgacgtc gtaggcgccg ccatacgcct
     1081 tgcgggtgat gaccgtcacc ttcggaaccg tcgcctccga ataggcgtac agcagcttgg
     1141 cgccatgccg aatgatgccg cggtgctcct ggtcgacgcc gggcaggaag ccgggcacgt
     1201 cgacgagcgt caccagcggg atgttgaagc agtcgcagaa gcggacgaaa cgtgccgcct
     1261 tgtcggccga gtcgatgtcc agcacgccgg ccagcacctc cggctgctgc gccacgatcc
     1321 ccacactgcg cccctcgatg cgcgagaagc cgcagatgac gttgcgcgcg aagtgcgcct
     1381 gcacctccgt gaacgagccg ccgtccacga tgccaccgat gacgcgatgc atgtcgtacg
     1441 cctgcgatgg cgagtccggg atgacggcgt ccaggtcctc cggagacatc gccggcggaa
     1501 gccactcctc catccgcggc gctgggtcga cgttgttcga cggccagtag ccaaccagcc
     1561 tgcgcgccag gtcgagcgcc tgcggttcgc cgggactgag aaagtgcgcc acgccgctcg
     1621 tctcgttgtg aacccgcgcc ccgccgagcc cctcgaaatc gatctgctca tgcgtcaccg
     1681 tcttcaccac gttagggccg gtgacgaaca tgtagctggt gccgtcgacc atgatcgtga
     1741 aatcggtgat ggccggcgag tacaccgctc cgccggcgca gggcccgagc accagtgaca
     1801 gctgcggcac cacacccgag gccagcgtgt tgcgcaggaa gatctcagcg tagccgccca
     1861 ggctgacgac tccctcctgg atcctggctc cgccggagtc ggacagaccg atgatcggcg
     1921 cgccgttctc catggccagg tccatgacct tcacgatctt ttccgcatgc gcctccgaca
     1981 gcgacccgcc gaacacggtg aagtcctgcg cgtagacgaa gacggtgcgg ccctcgatca
     2041 ggccgtggcc cgtgacgacc ccgtcgccca ggtactgctc ctggccgatg ccgaaatcgg
     2101 tggcccgatg ggtgacgaag gcatccagct caacgaagct ctccgggtcc aggagcagct
     2161 cgagccgctc acgcgccgtc agcttgccgc gctcgtgctg ctgagcgatg cggcgctccc
     2221 cgccgccctg catcgcctgc tcgcgcatgc gccgcaggcg ctcgagcttc tcggccgtcg
     2281 acaaggatgc ctccggaaat gtggtgggcc cagcagggat cgaacctgcg accagccggt
     2341 tatgagccgg atgctctgac cactgagcta tgggcccgcg ggacggatgg tggacaactc
     2401 cgagtatatc ggctcgtctt tgcacaaaga catcgacact cggcgacgtt gtggataact
     2461 cgggtgaccg tggccggttg ccgcggctat catcggtcct gcccgccgag acccggcgac
     2521 gcccccactc cgagccacga ctccgccgag gacgatgcgt tgaccaaggt tatctccatt
     2581 gccaaccaga agggcggcgt cggcaagacg accacctcca tcaacctggc gggtgcgctg
     2641 gcagagctcg gctaccgggt gctgtgcatc gacatggacc cgcaggcgaa cctgacggtc
     2701 ggcctgggca tctcgctatc cgacgtgcgg tactcgatgg gagacgtgct ggccgataat
     2761 cgcgtgccgc tggacgaggt cgttcgccaa acggagatgc cggggctgtc tgtggcgccc
     2821 gccaccctgg agctggccag cacggaggtc gagctgttca ctgccatcgg tcgtgagatg
     2881 gtcctgcgcg aagcgctgac cggctgggcg gagcgccagt tcgacgtggt catcatcgac
     2941 tgcccgccca cgctgggcct gctgaccatc aactcgctgg tcgccagcaa ccgggtcatc
     3001 atcccggtgc agacgcagta ctacgccatc aagggactga cggcgctgat caaggtgatc
     3061 aacaccatca agctcaagct taaccacgac ctggagatcc tcgggctgct ggcgaccttc
     3121 ttcgacgggc gtaccgtcct tgcccgcgag atgctgcaga acctgcgcga gatcggtgac
     3181 catcgggtct tcaacaccat gatcaagaac acggtcaagc tcggcgaggc cccgctgacc
     3241 ggcaagccgg tcacggagta cgcgaccaac tcggctgc
//
LOCUS       DATACA010000316         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_281022, whole genome shotgun sequence.
ACCESSION   DATACA010000316 DATACA010000000
VERSION     DATACA010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281022"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VHK28_06470"
     CDS             <1..313
                     /locus_tag="VHK28_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141907.1"
                     /translation="ALVVAAGVVVIGIGIYQVRKGLRSEFLGEIDLRTVAPALCTLTH
                     RDRTDGVLAVTVHCRSARDFVPAAFDAARAEGATISHVEVVPVTLEDVFISLTGRALR
                     E"
     gene            310..1155
                     /locus_tag="VHK28_06475"
     CDS             310..1155
                     /locus_tag="VHK28_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141908.1"
                     /translation="MTLQLLLGDLRAFGAAARKEWRILRRYPSLFLGFLFWPIALPTA
                     YVFQARGYAGDSQAALDAFAQRAGTVEIAGFLFVGWAAYMWISMILWGPGTALRTEQV
                     RGTLEAVFMTPVSRLVILFGPVVSQVIWALWMFAVVGGTLVLFFGVEITAPEVLRALG
                     VILVAVPALYGLGALFASVVLRFGEVSAAVQAVRGVFTVFCGMTFPIIILPEWGRAIA
                     LALPPTYLISDLRQVLLSSAELATLVPDLAITLALGLLLCGLAIIAFRRTERYARRGG
                     SLAQY"
     gene            1158..2006
                     /locus_tag="VHK28_06480"
     CDS             1158..2006
                     /locus_tag="VHK28_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141909.1"
                     /translation="MPPAFELSPRAELRAAWAIARKELQIARRYPLQLSNEVLQPLYQ
                     FLLPSLLLGATFFVGGRAVGLELLTGTDDLVGYLFLGMVVGGLVGTAFWDMGFGLKRE
                     MDAGTLEPAWLTPTRPETQVLSRAISGLIVAGGASVVLIGIGLVIFGATLGGAMLLAV
                     PALGLAAFSMVGIGYLIAAAVLVIKEPNFFVDATNFMFAMASGVAFPVVVLPWFIEPI
                     AYVLPTTYAVDLLRVYALGTRPLVDPALEWLLLAGFAALTIWLGRAVFLRTEHRMRVR
                     GTLGQH"
BASE COUNT          262 a          838 c          784 g          408 t
ORIGIN      
        1 ggcgctggtg gtggccgccg gcgtggtggt catcggcatc ggcatctacc aggtgcgcaa
       61 gggtctgcgg tcggagttcc tcggcgagat cgacctgcgg acggtcgccc cggccctgtg
      121 cacgctcacc caccgcgaca ggaccgatgg agtgctggcg gtcacggtcc actgccggag
      181 cgcgcgcgac ttcgtgccgg ccgccttcga cgccgcgcgg gcggagggcg ccaccatcag
      241 ccacgtcgag gtggtgccgg tgaccctgga ggacgtgttc atctccctca ccgggcgggc
      301 gctgcgcgaa tgaccctcca gctcctgctc ggcgacctgc gggccttcgg cgccgccgcg
      361 cggaaggagt ggcgcatcct gcgccgctac ccgtcgctct tcctgggctt cctgttctgg
      421 cccatcgcgt tgccgacggc ctacgtgttc caggcgcgcg gctacgccgg cgacagccag
      481 gccgcgctgg atgccttcgc ccaacgcgcc ggcacggtgg agatcgccgg cttcctgttc
      541 gtgggctggg cggcgtacat gtggatcagc atgatcctgt ggggcccggg caccgccctg
      601 cgcacagagc aggtgcgcgg cacgctggag gcggtcttca tgaccccggt ctcgcgcctg
      661 gtgatcctgt tcgggccggt cgtcagccag gtgatctggg cgctgtggat gttcgccgtg
      721 gtgggcggca ctctggtcct cttctttggc gtggagatca ccgcgccgga ggtgctccgc
      781 gcgctgggcg tgatcctggt ggcggtgccc gccctctacg ggctcggcgc cctgttcgcc
      841 tcggtggtcc tgcgtttcgg cgaggtgtcg gccgccgtac aggcggtccg cggcgtattc
      901 accgtcttct gcgggatgac ctttccgatc atcatcctgc ccgagtgggg tcgcgccatc
      961 gccctggcgc tgccacccac ctacctgatc tccgacctgc ggcaggtgct gctgtcgagc
     1021 gcggagctgg caaccctggt cccggacctg gccatcaccc tggccctggg cctgctcctg
     1081 tgcggcctgg cgatcatcgc cttccgccgg accgaacgct acgcgcggcg tggcggcagc
     1141 ctggcgcagt actgaccatg ccacccgcct tcgagctctc gccgcgcgcc gagctgcgcg
     1201 ccgcctgggc catcgcccgc aaggagctgc agatcgcccg acgctacccg ctgcagctct
     1261 cgaacgaggt gctgcagccg ctctaccagt tcctgctgcc cagcctgctg ctgggggcca
     1321 ccttcttcgt tggcggccgc gccgttggcc ttgagctgct gaccggaacc gatgacctgg
     1381 tgggctacct cttcctgggc atggtggtcg gcgggctggt gggcaccgcc ttctgggaca
     1441 tgggcttcgg cctgaagcgc gagatggacg ccgggacact ggagccggcc tggctgacgc
     1501 ccacccgccc cgagacgcag gtgctgagcc gcgccatctc cggcctgatc gtggccggtg
     1561 gcgcgagcgt ggtgctgatc ggcatcggcc tggtcatctt cggggccacg ctgggcggcg
     1621 cgatgctgct ggcggtgccg gcgctcgggc tggcggcctt cagcatggtg ggcatcggct
     1681 acctgatcgc cgctgccgtg ctggtcatca aggagccgaa cttcttcgtg gatgccacca
     1741 acttcatgtt cgccatggcc tccggcgtcg ccttcccggt cgtggtgctg ccctggttca
     1801 tcgagccgat cgcctacgtg ctgcccacca cctacgcggt ggacctgctg cgcgtgtacg
     1861 cgctcggcac ccgcccgctg gtcgacccag ccctggagtg gctgctgctg gccggcttcg
     1921 ccgccctcac catctggctg ggacgcgcgg tcttcctgcg aaccgagcac cgcatgcgcg
     1981 ttcgcggcac gctgggacag cactgaccga tagccgtcag tcctcagcca aggcgtaacc
     2041 gcccgcgaat cctcccccga agcgcccgac cgtcccgggc ccggtgttga tcacatctgg
     2101 actcacgagg gaggtcacca tgcgcaaccg tcacccgtca ccggtcgtcg tcgccaccct
     2161 gggcgccctg ctcgccctgc tgctggtggt cggcacggcc tccgcggcac ccaccaccct
     2221 gaccgccacc cttgccggcg gcgagggcat ggacaccgat ggctcgggca gtgccagcat
     2281 cacgctcgac cc
//
LOCUS       DATACA010000317         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1084661, whole genome shotgun sequence.
ACCESSION   DATACA010000317 DATACA010000000
VERSION     DATACA010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084661"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="VHK28_06485"
     CDS             <1..1653
                     /locus_tag="VHK28_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX2141910.1"
                     /translation="LAAVLSMLLALAVGALAFRLRSLRGEIFALLTLAVPFILAALAR
                     FNSAIDGGQGIIVTLPSFPDFIGGFQDLVYLVSLLIGWASVAIAFAMQRSRPGWALFS
                     IRDAEDVAEGLGVPTFRYKMVAIAITGMLAGAAGSVFALQVGFVTVENAFGLTVPLYV
                     IVMSVLGGRFHWLGPAIGAVVIVTLRDWLSAQGFEGWNLVILGTVLAVLILVAAEGLM
                     ARLGIRPRLTVGAFVGVLLVMAGLTATNIGPFGELETIDWVAAGMIAASVVAFWPARW
                     TRLRLVEPGEPEPAAEASVHPGEAQEAVAVAGAPIIEVVEVARHFGGIRALRGVSLTV
                     SEGELVGLVGPNGSGKTTLISLISGALAPTSGSIRIGGRETAGMEPHDIAHLGVARTY
                     QIPRPFATMTVRDNVAMALMFGREPLALGAARAAAEEHLELVGLTHRALALPAEINLH
                     ERQLLEMARAIATRPRVLLLDEALAGLNPAEIDNAAQVVRRIHASGIAIVLVEHLLRV
                     VNQLATRIVVLDQGLTLAEGAPESVMRNPEVVRAYLGRQAHA"
     gene            1646..2350
                     /locus_tag="VHK28_06490"
     CDS             1646..2350
                     /locus_tag="VHK28_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141911.1"
                     /translation="MLEVRDLNVHYGDAAALWSVDLDVADGEVVSIVGPNGAGKSTLV
                     NTIAGLHRPSSGRIVMDGHDVTRVASHRVCGHGLAIVPEGRRIFPGMSVRDNLDLGAY
                     RSEARDRHAESLDWVLGLFPRLGERSAQRAGTLSGGEQQMLAVGRALMARPRLLLMDE
                     PSLGLAPVIVDEVFDVIRTIHDAGVSVLLVEQNAQRAFDVSARGYLLSEGRIVLSGTS
                     GELAANDDIRRLVLGV"
BASE COUNT          363 a          969 c         1018 g          457 t
ORIGIN      
        1 ctggcggccg tgctgtccat gctgctggcc ctggccgtgg gcgccctggc cttccggctg
       61 cgctccctgc gtggcgagat cttcgcgctg ctgaccctgg cggtgccctt catcctggcc
      121 gcgctggccc ggttcaactc ggccatcgac ggcggtcagg gcatcatcgt caccctgccg
      181 tccttccccg acttcatcgg cggcttccag gacctggtct acctggtcag cctgctcatc
      241 ggttgggcgt cggtggccat cgccttcgcc atgcagcgct cgcgcccggg ctgggcgctc
      301 ttctccatcc gggacgcgga ggacgtcgcc gagggcctgg gcgtgcccac cttccgctac
      361 aagatggtgg ccatcgccat caccggcatg ctggccggcg cggcgggcag cgtcttcgcc
      421 ctgcaggtcg gcttcgtgac ggtggagaac gccttcgggc tgaccgtgcc gctgtacgtg
      481 atcgtgatga gcgtgctcgg cgggcgcttc cactggctgg gcccggccat cggtgcggtg
      541 gtgatcgtca ccctgcgcga ctggctgtcg gcacagggtt tcgagggctg gaacctggtc
      601 atcctgggaa cggtgctggc ggtcctgatc ctggtcgccg cggaggggct gatggcccgc
      661 ctgggcatcc ggcccaggct gaccgtgggc gccttcgtcg gggtcttgct ggtgatggcc
      721 gggctgacgg cgacgaacat cggtccattc ggtgagctgg agaccatcga ctgggtcgct
      781 gccggaatga tcgccgcgtc cgtggtcgcc ttctggcctg cccgatggac ccgccttcgc
      841 ctggtcgagc ccggcgagcc ggagccggcc gccgaggcat cggtccaccc cggtgaggct
      901 caggaggccg tggcagttgc gggcgcgccg atcatcgagg tcgtggaggt ggccaggcac
      961 ttcggcggga tccgcgcgct gcgcggcgtg tcgctgacgg tgagcgaggg cgagctggtt
     1021 gggctggtgg gacccaacgg ctccggcaag acgacgctga tcagcctcat ctcgggggcg
     1081 ctggcgccca cctcgggcag catccggatc gggggccgcg agacggccgg gatggagccg
     1141 cacgacatcg cgcacctggg cgtggcgcgc acctaccaga tcccgcggcc cttcgcgacc
     1201 atgaccgttc gcgacaacgt ggccatggcc ctgatgttcg gccgcgagcc gctggctttg
     1261 ggggcagctc gcgccgccgc cgaggagcac ctggagctgg tggggctgac gcaccgcgcg
     1321 ctggcgctgc cggccgagat caacctgcac gaacgccagc tgctcgagat ggcgcgcgcc
     1381 atcgccaccc gtccgcgggt gctgctgctg gacgaggcgc tggccggcct caaccccgcc
     1441 gagatcgaca acgcggcgca ggtcgtgcgg cgcatccacg cctccgggat cgcgatcgtg
     1501 ctggtggagc acctgctgcg cgtcgtcaac cagctcgcca cccggatcgt ggtgctggac
     1561 caggggctga ccctggccga gggggcgccg gaatcggtca tgcgcaaccc ggaggtcgtg
     1621 cgcgcctacc tggggcggca ggcccatgct tgaggtccgg gacctgaacg tccactacgg
     1681 cgatgccgcc gccctctgga gcgtggacct ggacgtggcc gatggcgagg tggtcagcat
     1741 cgtggggccc aacggtgccg gcaagtcgac gctggtcaac accatcgccg ggctgcaccg
     1801 gccgtcgtcc ggccgcatcg tcatggacgg gcacgacgtc acccgcgtcg cctctcaccg
     1861 ggtttgcggc cacgggctgg cgatcgtgcc cgagggacgc cgcatcttcc cgggcatgtc
     1921 ggtccgggac aacctggacc tgggcgccta ccgctccgag gcccgggacc gtcacgccga
     1981 atccctggac tgggtgctcg gcctcttccc gcggctgggg gaacggtccg cgcagcgggc
     2041 cggaaccctg agcggtggcg agcagcagat gctggcggtg ggtcgcgctt tgatggcccg
     2101 gccgcgtctc ctgctcatgg acgagccatc cctgggcctc gccccggtga tcgtggacga
     2161 ggtcttcgac gtcatccgca ccattcacga cgcgggcgtc agcgtcctgc tggtggagca
     2221 gaacgcccag cgcgccttcg acgtctcggc gcgcggctac ctcctcagcg agggccggat
     2281 cgtgctctcc ggaacctcgg gggagcttgc cgccaacgac gacatccgcc gcctggtcct
     2341 tggcgtctga cgcgtgcggg tcgtccccgg cgcggcgccg atgcagcggg caggttttca
     2401 ccggagggcg ccgccgcgtg ctgatctgcc gttctatcgg gcagccgtga gcgtgagggg
     2461 cgaagaactt gtcgcttcgt gctgggacct gcccgctgta tcggcagatt gacacgtagc
     2521 caacggcccg cgtgaaagac tgcccgaaat agcgacgggg cagctccccg ggccgcaccc
     2581 acgaggcagc ctctcagcga tgcccggccg ccggccctct tgagcggcca catctgtggg
     2641 aaatgcagca tgcggtgatg cataacaacc acgacggtcg gccggagcgg caggatcctc
     2701 gccgtcacgc ctcaccggta ccgcatttcc caaggatctc gcgcgaagag ggtcggatcg
     2761 tagagaagct gcggtgtccg cacggcagcg ccggatgcag tgggcag
//
LOCUS       DATACA010000318         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_844086, whole genome shotgun sequence.
ACCESSION   DATACA010000318 DATACA010000000
VERSION     DATACA010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844086"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..958
                     /locus_tag="VHK28_06495"
     CDS             377..958
                     /locus_tag="VHK28_06495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2141912.1"
                     /translation="MSPIAVTNAELLGGLAIEILLTATVGIWLWRSGWRPHREATLPL
                     QSQDMARGLGLWAGAILSVMGWALVCRILWPGLLAIAEQTQITGAPHFLVSVAFSVYN
                     ALFEELLWLALGITAFRRFGIATAAGISIALRLLAHAYQGPLALVTILPIGIIFTVYY
                     LRTRRLWPVVIAHAFQDVLALSFLASSAGRAPA"
     gene            complement(1283..1858)
                     /locus_tag="VHK28_06500"
     CDS             complement(1283..1858)
                     /locus_tag="VHK28_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141913.1"
                     /translation="MYNTMRIPASITAFSLALASNLGAQEAEDPQRVTLLGLRHIAAH
                     AKVQVARQATLDPVDDAVLRTRLEQALRQEGIVVQEHKDVRDGSAGQISLVYTVISMA
                     DASGRETRFAASSCLHASQYVRIPRLERGGRVAYTVAPTWSSCGIVAGSTDSYRNAIL
                     LNADEQIARFLEAWRSVNPRGSAAPRILSSE"
     gene            complement(1970..2323)
                     /locus_tag="VHK28_06505"
     CDS             complement(1970..2323)
                     /locus_tag="VHK28_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX2141914.1"
                     /translation="MASALVPGATVAWVTSKLGLQTREPPAPQAVLAIESRQPLQGEL
                     MSFYIDEALVVTGMPIEELPLPEESAVTLIVRKDKLLPPKPGTTLQPGDHVHLIAQPE
                     DRGLIQLMFGRPEEE"
     gene            complement(2354..3451)
                     /locus_tag="VHK28_06510"
     CDS             complement(2354..3451)
                     /locus_tag="VHK28_06510"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX2141915.1"
                     /translation="MTPNEPEATALLLAVGGLLLGVSVVFSRASQRTGLPIALLFLVV
                     GMLAGSEGIGGIEFDDYEFAFRIGTLALALILFDGGLNTPLSAVRRVAAPVGILATVG
                     VAMVAGLTAVVAHLLGLGWGEALLVGAVVSSTDAAAVFSVLRGSGLHLKRRVGVTLEV
                     ESGINDPAAIILTTVLTQNLLAPGSVGGWQIPLQVLLQIVVGTAVGLGVGYGGRELLS
                     RLLLSTGGLYPVMTLALGLLSFGVTTLLSGSGFLAVYLTGLIMGNGPLPYRPGLLRVH
                     DALAWLSQVGMFLILGLLVFPSRLLDVAAVGLGISLLLALVIRPLVVAVILAPFHYRV
                     REVLYIGWVGLRGAVPIVLATFPVLVGAPGA"
BASE COUNT          599 a         1184 c         1194 g          544 t
ORIGIN      
        1 gacccacacg gcgctccacg cgatcggcca gcgggtccac tccgaatcag caggcataga
       61 ggctcctttc cccagggagg ccgggttagc gattcctagc ctgcctggcg cagggccgct
      121 gtggcggggg tcaccagggg cgggcggcca ggcggtcagg cggtcaggcg gacgggcgga
      181 cgggcggacg ggcggacggg cggagactac aggttaggtt gtcgtgcact atttcagtga
      241 gggccagagt gctccggaaa gaatggcgta cccacaggta gctggcggaa tctcggaacg
      301 gaaagccgcc gtcgggctgc tgattcccgt gctcctgatg gcagcgggga cggcgctgca
      361 gaggagtgac gggagcatga gcccgatcgc cgtcaccaac gccgagctcc tcggcggcct
      421 ggccatcgag atcctcctca ctgccaccgt cgggatctgg ctctggcgtt caggctggcg
      481 gccgcaccgc gaagcgacgc tgccgctcca atcgcaggac atggcccggg ggctgggcct
      541 ctgggctggc gccatcctgt cggtgatggg ctgggcgctg gtgtgccgga ttctctggcc
      601 cggcctgctc gccatcgccg agcagacgca aatcaccgga gcgcctcatt tcctggtctc
      661 cgtcgccttc tcggtctaca acgccctctt cgaggagctg ctgtggctcg cgctcggcat
      721 caccgcattt cggcgattcg gtatcgccac tgctgcgggg atcagcatcg cgctccggct
      781 gctggcgcac gcctaccagg ggccgttggc gctggtcacc atcctgccga tcggcatcat
      841 ctttacggtg tactacctgc ggacacgccg gctctggccg gtcgtcatcg cgcacgcatt
      901 ccaggacgtg ctggcgctca gtttcctggc gagctcggcc ggacgcgctc cggcgtaggg
      961 ctttcgcggt cgctgcttcg ggtacgatcc atgggcgggc gcggggcagg gtcgccgccg
     1021 cggaactcga agtgggtggg gtcgaaggtg cgcgtgtgga ggccttcccg ccgccgggcg
     1081 tgggctcgct cgaccagccg cctggcgagc tcggtggccg gcatccacag gcccgccggc
     1141 atgtcggggt actctgccgc aaactcaggc ctgagccgca cctcacgctg attcacgaat
     1201 atccgccgta tctggtcacc gacgactagt gtcggtctac ccgaggtgaa cttgagaggc
     1261 ggagaggcgg agaggccgag tgtcactcgc tcgacaggat ccggggcgcc gccgatccgc
     1321 gcgggttgac tgagcgccag gcctcgagga atctcgcgat ctgctcgtcg gcgttgagaa
     1381 ggatggcgtt tcgataggag tcggtgctcc cggccacgat gccgcagctg ctccaggtcg
     1441 gcgccacggt gtaggcaacc cgtccacccc gttcgagccg gggaatccgc acgtactggg
     1501 aggcatgaag acaggacgac gccgcgaacc gggtctcccg gcctgacgcg tcggccatgg
     1561 atatcaccgt gtataccaga ctgatctgtc ccgcggagcc gtcgcgaaca tccttgtgct
     1621 cctgcacgac gatcccctcc tgccgcaggg cctgctccag cctggtacga aggactgcgt
     1681 catcgaccgg gtcgagagtg gcttgcctag ccacctgtac cttcgcgtga gcggcgatgt
     1741 gtctgagacc aaggagagtg acccgctgcg gatcttcggc ttcctgggcg ccgagattgg
     1801 aagcgagcgc cagcgagaag gcagtaatcg aagcgggaat tcgcatcgtg ttgtacatat
     1861 ggtttctcat ggctcggctc gctctgctgt ccgccagtcc gccagtccgc cagtccgcca
     1921 gtccgcccgt ccgccagtcg gcctgaccgc ctgcccgcct gcccgccgcc tactcctcct
     1981 ccggtctgcc aaacatcaac tggatcaacc cgcggtcttc gggctgcgcg atcaaatgca
     2041 cgtggtcgcc cggctggagc gtggtgcccg gcttcggcgg gaggagcttg tccttccgca
     2101 cgatcaacgt gacggccgat tcttccggca ggggcagctc ctcgatcggc atcccggtca
     2161 ccaccagcgc ttcatctata tagaacgaca tcagctcgcc ctggagcggc tggcgcgact
     2221 cgatggcgag cactgcctgg ggcgccggcg gctccctggt ctggaggccg agtttgctcg
     2281 tgacccacgc gacggtcgca cccggcacca gcgcgctcgc caccacgatg aagaagacca
     2341 ggtcgaagag ccgctaggcc ccaggagccc ccaccagcac cgggaaagtg gccagcacga
     2401 tcggaactgc gccccggagc cccacccaac caatgtagag gacctcgcgc acccggtagt
     2461 ggaacggcgc gaggatgacg gcaacaacca gtggccggat gaccaatgcg aggaggagcg
     2521 agatgcccaa ccctactgcc gctacatcca gcaagcggga gggaaagacc agaagaccga
     2581 ggatcaggaa catccccacc tgactcagcc aggcgagcgc gtcgtggacc cgcaggagcc
     2641 cgggccggta tggaagcggg ccgttcccca taatcagccc ggtgaggtag accgccagga
     2701 agccgcttcc actcaggagc gtggtgacgc cgaagctcag gagtccaagc gccagagtca
     2761 tgacggggta gagcccaccg gtcgagagca ggagacggga cagcagctct ctcccgccgt
     2821 accccacgcc gaggcccacc gcggtgccga ccacgatctg aagcaggacc tgcagcggga
     2881 tctgccaccc gcccacgctg cccggcgcca acaggttctg cgtcagtacg gtggtgagaa
     2941 tgatcgccgc cgggtcgttg atgccggact cgacctcgag cgtcacgccg acccggcgct
     3001 tgaggtggag cccgctgccc cgtaggacgg agaacaccgc cgccgcgtcg gtggaggaga
     3061 ctactgcccc gaccagcagc gcctcgcccc agccgagccc caggaggtgg gcgaccacgg
     3121 cggtgaggcc ggcaaccatc gcgacgccta ccgtcgccaa aatcccgacg ggagcagcta
     3181 cgcggcggac ggcactcagc ggggtgttga ggccgccatc gaacaggatg agcgcgagcg
     3241 ccagcgtacc gatgcggaag gcgaactcgt agtcatcgaa ctcgatcccg ccgatcccct
     3301 cggagccggc cagcatgccc acgaccagga acaacagggc tatgggcagg ccggtgcgct
     3361 gcgaggcccg gctgaacacc acgctcacgc cgagcagcag cccgccgacg gcgagaagga
     3421 gcgcggtggc ttcgggctcg ttgggagtca ttggtcgcta tgggctcgct actggtatca
     3481 ggcgactggc tattcgctgg tggctacgcc gctgaccagc c
//
LOCUS       DATACA010000319         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1567660, whole genome shotgun sequence.
ACCESSION   DATACA010000319 DATACA010000000
VERSION     DATACA010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567660"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VHK28_06515"
     CDS             <1..341
                     /locus_tag="VHK28_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2141916.1"
                     /translation="LDAVAALGAEQLVGGRGPVVRGVAVRQAIDDTRAFLTAVLDGTR
                     EVRDAGGTPRDAYRATHAALAPRYGSYPIFEHTMPFNVQRCWDELEGIDHPRIWTVER
                     DRAVWDELTS"
     gene            361..2070
                     /locus_tag="VHK28_06520"
     CDS             361..2070
                     /locus_tag="VHK28_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX2141917.1"
                     /translation="MDRHPRDAQVAVVGAGPIGLTLAGRLAQRGVRVLLLEQAPHHQG
                     EGSKALCMQRETLEIWERLGIGQRVADRGIQWSVARTYFRSRELFSVKLPDGEGDHFP
                     AFVNISQTEVEELLIGRLEELSGCQVRWGHRVTGIAQDSAGVVVRCETPEGERTFSVD
                     YLVGTDGAHSSVRHLLGMGFPGHSHEDLFLICDIRADLPFPRERRFFFDPPWNPGRQV
                     LVHPQPDGVWRIDWQVPPGTDADAELTNGGLDRRIRAIVGDQPYELLWMTAYHFHQRM
                     VERFRAGRVLLAGDAAHLMSPFGARGLNSGTADAENLAWKLAAVLSGDAPERLLNSYH
                     AERRAAAVENLAVTEATMRFMVPHGPLRRVWRDVVLRGSVRAGPLRRRVNSGRLAQPF
                     AYAGSPVIQPGPDDPRLPAHGDVAPDAACALLRADPGERPVTRLRDLVGHGFLVLLAC
                     GERPDLAVAAAVRAARLAWPTDVRVAVVGAGTVPGGVLLLDDVRGELASIYAAAGPRA
                     WLIRPDGHLAGSLPLHAREAVDGLPGLLARAIGASAASAPAAVRPAPRRRVSVSAGNR
                     AGH"
     gene            complement(2073..2678)
                     /locus_tag="VHK28_06525"
     CDS             complement(2073..2678)
                     /locus_tag="VHK28_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141918.1"
                     /translation="MSVLHMARGWRARTLHAGAAWLAVLLLAACTSPAASPSADDLTP
                     TATAAASEAEPMSEAPSESAEASEPAAMDDATVTVADSDLGEILVDAEGMTLYVFTND
                     TEGTSNCSGDCLTNWPPLTVDEGEDPVAGEGVTAELATIERDDGSIQVTANGLPLYFF
                     AGDQAAGDLNGQNVGGVWFVVGPDGEMIQETAAVPDNPYDY"
     gene            complement(2927..3517)
                     /locus_tag="VHK28_06530"
     CDS             complement(2927..3517)
                     /locus_tag="VHK28_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2141919.1"
                     /translation="MPPRLVAIGLLVLMAGCAPSLTLTDGTSARTWGDGAYGVVVVPD
                     ADRGAASWEPIGSAWAEEGMTVLAVEEPIADAVLGGIRHLHEAGVERVAVLGAGAGAE
                     PAMAVGREQPDLVDQLIVVSARGSAEGLGVFPKLFVASEGEPAAADATRMAAESPGDW
                     NALYLASGDASGQAILEGDAAAATLEAILLRLEERR"
BASE COUNT          500 a         1318 c         1332 g          519 t
ORIGIN      
        1 cgctggatgc cgtcgccgcc ctgggcgcgg agcagctggt cggcgggcgc ggccccgtcg
       61 tccggggagt ggctgtccgc caggcaatcg acgacacccg cgccttcctc acggcggtgc
      121 tggatggcac gcgcgaggtg cgcgacgccg gcggcacccc acgggacgcc taccgtgcca
      181 cgcatgcggc cctcgccccg cgctacggca gctatcccat cttcgagcac accatgccgt
      241 tcaacgtcca gcgctgctgg gacgagctcg agggcatcga ccatccgcgc atctggacgg
      301 tcgagcggga ccgcgcggtc tgggacgagc tgacgagctg acgggctgac ggagcggcag
      361 atggacaggc acccccgcga cgcccaggtg gcggtggtgg gtgccggacc catcgggctc
      421 acgctggccg gccggctggc gcagcggggg gtgagggtcc tgctgctgga gcaggcgccg
      481 caccaccagg gagagggctc caaggcgctg tgcatgcagc gcgagacgct cgagatctgg
      541 gagcggctgg gcatcggtca acgggtggcc gaccggggta tccagtggag cgtggcgcgc
      601 acctacttcc ggagtcgcga gctgttcagc gtcaagctgc ccgacggcga gggcgaccac
      661 ttcccggcct tcgtgaacat cagccagacc gaggtcgagg agctcctcat cggtcgcctg
      721 gaggagctct cgggctgcca agtgcgctgg gggcaccggg tgaccggcat cgcccaggat
      781 tccgccggag tcgtggtccg ctgcgagacc ccggagggcg agcgcacctt cagcgtggac
      841 tacctggtcg ggaccgatgg tgcccactca tcggtccgtc acctgctggg catgggattc
      901 ccgggtcaca gccacgagga cctgttcctg atctgcgaca tccgcgccga cctccccttc
      961 cccagggagc ggcgcttctt cttcgacccg ccgtggaacc cgggccggca ggtgctggtg
     1021 catccccagc cggatggcgt gtggcgcatc gactggcagg ttccacccgg gaccgacgca
     1081 gacgcggagc tcaccaacgg cggcctcgac cgccgcatcc gtgccatcgt cggcgaccag
     1141 ccgtacgagc tgctgtggat gaccgcctac cacttccacc agcggatggt ggagcgcttc
     1201 cgcgccgggc gcgtcctgct ggcgggggac gcggcgcacc tgatgagccc cttcggggcg
     1261 cgcggcctga actccgggac ggcggatgcc gagaacctgg cctggaagct ggccgccgtc
     1321 ctgtccgggg acgcgccgga gcggctgctg aacagctacc acgcggagcg ccgggcagcc
     1381 gcggtcgaga acctggccgt caccgaggcc accatgcgct tcatggtccc gcacggcccg
     1441 ctgcggcgcg tgtggcgcga cgtcgtgctg cggggcagcg tgcgggccgg gccgctgcgc
     1501 cgcagggtga actcggggcg gctcgcccag cccttcgcct acgccgggtc gccagtgatc
     1561 cagcccgggc cagacgaccc tcggctgccg gcgcatggcg acgtggcgcc ggacgcggcg
     1621 tgcgcgctcc tccgcgcaga ccccggggag cggccggtca ccaggctgcg cgacctggtc
     1681 ggccacggct tcctcgtgtt gctggcctgc ggagagcgcc cggacctggc ggtggcggcc
     1741 gcggtgcgcg cggcgcgcct cgcctggccg accgacgtgc gggttgccgt ggtcggggcc
     1801 ggcacggtgc ccggcggcgt gctgctgctc gacgacgtgc gcggcgagct ggccagcatc
     1861 tacgccgccg ccggaccacg tgcctggctc atccggccgg acggccacct ggcgggcagc
     1921 ctgccgctcc acgcgcgcga ggcggtcgac ggcctacccg ggctgctggc gagggccatc
     1981 ggagcgtccg ctgccagcgc tccggcggcg gtgcgaccgg ccccccgccg gcgcgtcagc
     2041 gtctcggcgg ggaaccgggc ggggcactga gcctagtagt cgtacgggtt gtcggggacg
     2101 gccgctgtct cctggatcat ctcgccatcc ggacctacca cgaaccacac gccacccacg
     2161 ttctgcccgt tcaggtcgcc ggcggcctgg tcgccagcga agaagtagag cggcagcccg
     2221 ttggccgtga cctggatcga gccgtcgtcg cgctcgatgg tcgccaactc ggcggtgacg
     2281 ccctcaccgg ccactggatc ctcgccctcg tcgacggtga gcggcggcca gttggtcagg
     2341 cagtcaccgg agcagttgct ggtgccctcg gtgtcgttgg tgaagacgta cagggtcatg
     2401 ccctcggcat cgaccaggat ctcgcccagg tcggagtccg cgaccgttac cgtggcatcg
     2461 tccatggcgg ccggctcgga ggcctccgcg gactcgctcg gcgcctcgct catcggctcc
     2521 gcttcgctcg ccgccgccgt ggcggtggga gtcaggtcat ccgccgatgg cgacgctgcc
     2581 ggggaggtgc aggccgcgag cagcaggacg gccagccagg cagcgccagc gtggagggta
     2641 cgcgcccgcc acccgcgggc catgtgaagg acggacatgg tcgtgcgttg cctcgttcgg
     2701 tgattcggat cctcaggtag gtcgttcgcg cccggttacg cgctggattg gggacgcgat
     2761 gtggataagt cgatgcgatt gcctccaagt tggtggataa ctgccttgtg cggttaggtg
     2821 ctgcctgcca cacttcggtt gtcccgaagg ggcagctggc cgactggaga tcacatcaac
     2881 ggtcgccgcg gttccttcgg gacatttcca tgcccgccga ttggtactag cggcgctcct
     2941 ccagccgcag caggatcgcc tcgagggtag cggcggccgc atctccctcc aggatcgcct
     3001 ggcccgatgc gtcgcccgat gccagataga gggcgttcca gtctccgggc gactcggcag
     3061 ccatgcgggt ggcgtcggcg gccgcgggct ctccctcgga tgccacgaac agcttgggga
     3121 agacgcccag cccctcggcc gagcctcgtg ccgagaccac gatcagctgg tcgaccagat
     3181 ccggctgctc gcggccgacg gccatcgccg gctccgcgcc ggcgccggcg cccagcaccg
     3241 ccacccgctc cacgccggcc tcgtgcaggt gtcggatgcc gcccagcacc gcgtcggcga
     3301 tcggctcctc caccgccagc acggtcatgc cctcctccgc ccatgcgctg ccgatcggct
     3361 cccaggaggc ggcgccgcga tccgcatcgg gcaccaccac cacgccgtac gcgccgtcgc
     3421 cccaggttcg ggcggaggtg ccatcggtca gggtcagcga cggtgcgcag ccggccatca
     3481 acaccagcag cccgatggcg acaagccgcg gaggcatcgg tccagggccc tagaaggccc
     3541 acgccccgct gctgaagcgc agggcgaagt acacgaacag gcccagcgac accgtcgaga
     3601 tggcgaccag cacgcctgcg cggcggctga cgagtgccat ggccacgaac agcgggaaga
     3661 gggtcaggc
//
LOCUS       DATACA010000320         8392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1486416, whole genome shotgun sequence.
ACCESSION   DATACA010000320 DATACA010000000
VERSION     DATACA010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486416"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VHK28_06535"
     CDS             complement(<1..1002)
                     /locus_tag="VHK28_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX2141920.1"
                     /translation="MQRPVEDHEAIVIGAGQAGLAAGYHLARRGIEFQILEASPRVGD
                     VWRDRYDSLRLYSPAKYDALPGYPMPLRRSQFPTGVQMADYLEAYAAHHRLPVRTGMR
                     VHSLRADADGAGYEIEAGGRTLHANQVIVASGAFQRPHVPTFASELGADIRQIHSADY
                     RNPRQLADGPVLVVGVSHSGADVAYEAARAGHRTFLSGRPHGQLPFSVDSRRARVAWP
                     LLRFVAMNLLTLSTPIGRKMAPRVRHGGGPLLRIRRADLQRVGVEWNEARTTGVRNGL
                     PVLDDGTVLEVANVIWCTGFRPDYSWIELPVTDADGWPMQERGVVHSAPGLYFLGTPF
                     "
     gene            1116..1856
                     /locus_tag="VHK28_06540"
     CDS             1116..1856
                     /locus_tag="VHK28_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX2141921.1"
                     /translation="MGSSAKTEYCAFTKAVEHLGDRWSLLIVRELAMFGPQGFNTLAQ
                     GLPGHVSRSVLAEKLRKLEEVGLLARDPRRPSGPAPYCLTPAGEQLMPTLKSLWEWAE
                     KWVPEDPRMAQRDPTIILWWLTHRVDVAAGPERQVALEINMEAPGAERVWLLLARGTE
                     PTLCLEDPLLGEDRYVYLEADGDRLYPIARGLRSWSDALADGSVRAYGEPSLVQALPG
                     WFVPAGSQHRNGEGRAEVAPEPVSALSS"
     gene            complement(1900..2559)
                     /locus_tag="VHK28_06545"
     CDS             complement(1900..2559)
                     /locus_tag="VHK28_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141922.1"
                     /translation="MGETLDETRLEVTAQRAELERQAAALEARVRHALDFKARFRENP
                     ALFIGLGAGAVFLVAGGPVRVARLLRRRMRPTTAEQAYDALPKGLQHWVDTVSGELGP
                     KTDKARLAITEELLRWRNDPFKNKKARKELARAMVDGPPGAGRAGWKAAEAALTLLSA
                     ALARRAIEAFLTGERPIRPSVPSSALPSGEELRKGAPKDPKEAAREASVEYTGFSSRG
                     R"
     gene            complement(2529..2933)
                     /locus_tag="VHK28_06550"
     CDS             complement(2529..2933)
                     /locus_tag="VHK28_06550"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2141923.1"
                     /translation="MTQGEPAPRQSIFGQARQLISGVIQLARLEVTRGRQEIGQMLAE
                     TKSGLMLIGVALVIVLLALITFVMLIVLAIAALTGLPGWLVALIVLVVFIGLAILLAW
                     LGIRRIRIGPPEETIEAVKEDIAWAKRLMRRG"
     gene            3077..3721
                     /locus_tag="VHK28_06555"
     CDS             3077..3721
                     /locus_tag="VHK28_06555"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEX2141924.1"
                     /translation="MAKRRTSGIQRRRASNDALTRPLDGRGSSPALDPRILIVGGVLV
                     VGAIVLVVVLLFGGGGAAPVGTQVPDEGAGHVEQGEFPTYQSRPATSGPHWNVANLAP
                     MNWGVYTSPVPEPGVVHNLEHGGIVIWYQPNETSAEDVQALTEFVEQQIRGSRFKVIL
                     SPWAGEDFGSPIAVTAWNWVMYLNTADIDQIRAFIDDHYQQAPENFGGPGQPAT"
     gene            3786..3860
                     /locus_tag="VHK28_06560"
     tRNA            3786..3860
                     /locus_tag="VHK28_06560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3818..3820,aa:Lys,seq:ctt)
     gene            complement(3867..6086)
                     /locus_tag="VHK28_06565"
     CDS             complement(3867..6086)
                     /locus_tag="VHK28_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX2141925.1"
                     /translation="MTKARLKVGAERPKPLDLLHTDPLVAPSYEVQAGGDPGEEHDQV
                     LKHAESFAVFDRHGDVQASGLGEEGIYHLGTRHVSSLQLLMAGRRPLLLGSTAQRDNS
                     RLAVDLTNGDVGDGADRIPHSSVHLSRTKVLWDGVLYERLVARNFTRRPLRIPLTLRF
                     QADFADIFEVRGMERAERGRRLTTQVSERVVVLRYAGLDGVMRRSRITFSRPPDLLEE
                     GQASFHLHMPDARAVTIEVAIACETGSRRRSVLSSVDEAFDRARGHLPHVLGDAASVT
                     SSNELFNDWLDRSVADVAMLTSRTSDGLYPYAGVPWFSAPFGRDALVTGFQTLWVAPR
                     IAQGVLGYLAARQAAQVDPEADAEPGKILHEARNGEMAALGEIPFRRYFGSHDATPLF
                     VMLAAAHWRQTDDAVTAERLWPHVERALEWMDTYGDPDGDGFLEYARHRPDGLVQQGW
                     KDSHDSIFHEDGRLADAPIALCELQGYAYAAKRGAADLAELLGHGERASQLRGQASEL
                     RDRFDAAFWDEELGTYVLALDGDKRACRVRASNAGHALMTGIALPSRAERVAEALMAD
                     SSFSGWGVRTVAAGAARYNPMSYHNGSVWPHDNALVAMGLARYGRHDAAARMLTALFD
                     ASRYFDLARLPELFCGFTRRDGEGPTRYPVACSPQAWAAGAVFMLLQASLGMEIDAPA
                     HQVRFSYARLPEFLDRLSISGLRVGEASVDLELERQTHGVGFRVLHRSGDVDVVAVK"
     gene            6225..7541
                     /locus_tag="VHK28_06570"
     CDS             6225..7541
                     /locus_tag="VHK28_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2141926.1"
                     /translation="MPHVPIQRHRLPNGLRVILSPDDLAPIVAVNLWYDVGSKHEKPG
                     RTGFAHLFEHMMFQGSEHVAKGEHFALVQAAGGTLNASTWLDRTNYFETLPSHELELG
                     LWLESDRLASLLPAMTQEKLDNQREVVKNERRWSVDNQPYGTWDEKLQALLYPETHPY
                     HHSTIGSMDDLNAASLDDVREFFATYYAPNNAVLTVAGDFEPATALAMIERHFGPIPA
                     NPNLPPAPDMSVAALLGDEVREVVPDRVPLPRVYVGFRMPVFGSSEFEALSVAADALG
                     VGRASRLYRVLVRERQLAQDVTVFPFPVVGGASMFTMWATARPGVPSADLEAAMLAEI
                     DHLAADGPSDAELERVRNLHASAVESSLERIAERADRLSMYTCLFDEPDRINTEVSRY
                     LAVDASRVREAMAAVLRPDNRLVLTYVPAEAPMDPTDAPAATEEVT"
     gene            7538..>8392
                     /locus_tag="VHK28_06575"
     CDS             7538..>8392
                     /locus_tag="VHK28_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX2141927.1"
                     /translation="MTLTTNPLRPTPGAPRPYRFPEFDRRRLDNGLTVWMVPLPAAHV
                     ASVHLLVDAGAAAEEEARAGVAALTAQLLVTGTRRLDASAFAEATERLGIEVSSESSW
                     DSARAAFQSLPEHIEAGMALLAEMVREPRLDPAEFDRLRAERLADILQSRADPGRLAE
                     EMFLRHTYDDTTPYRRLSAGVPETVSTLSLDDVRAHHETNWRPGNAHLIVVGWIDPEA
                     VMRAAQRAFGDWEGTAAGHRTLHPAPAGDRRVVIVDRPGSVQSELRIGHIGIERTSDR
                     YFPAMVMGA"
BASE COUNT         1237 a         3034 c         2891 g         1230 t
ORIGIN      
        1 gaagggggta cccaggaagt agagcccggg cgcggaatgg acgacgccgc gctcctgcat
       61 cggccagcca tcggcatcgg tgaccggcag ctctatccac gagtagtcgg ggcgaaagcc
      121 ggtgcaccag atgacgttgg caacctccag cacggtgccg tcgtccagca cggggagccc
      181 gttgcggacg ccggtcgtgc gcgcctcgtt ccattccacg cccacgcgct gcagatccgc
      241 gcgacggatg cgcagcagcg gcccgccgcc gtgcctcacc ctgggggcca tcttgcgccc
      301 gatcggcgtg ctcagggtca gcaggttcat ggccacgaag cgcagcagcg gccaggccac
      361 gcgggcacgc cggctgtcca ccgagaacgg aagctgaccg tggggcctgc cagacaggaa
      421 cgtccgatgg cccgcccgcg ccgcctcgta ggcgacgtcc gcaccggagt ggctgacgcc
      481 caccaccagc accggcccat ccgccagctg ccgtggattg cggtagtcgg cggagtggat
      541 ctgccggatg tccgcgccca gctccgacgc gaaggttggg acgtggggtc gctggaacgc
      601 gccgctggcc acgatgacct ggttggcatg cagcgtcctg cctccggcct cgatctcgta
      661 gccggctcca tcagcatcgg ctcgcagcga gtggacgcgc atgccggtac gcaccggcag
      721 ccggtgatgt gcggcgtacg cctccaggta gtccgccatc tggacgccgg tcgggaactg
      781 gctgcgccgc agcggcatcg ggtacccggg cagcgcgtcg tacttggcgg gcgagtacag
      841 gcgcaacgag tcgtatcggt ccctccagac gtcccccacc ctggggctgg cctccaggat
      901 ctggaactcg atcccgcggc gggccaggtg atagccggcg gccaggccgg cctgtcccgc
      961 cccgatgacg atcgcctcgt ggtcttcgac tggcctctgc atcggtccgt tctccttgct
     1021 ggaatgtcct ggagcgcagg gtccgcttga gcgccggaac cgcctagttc gaagagcgaa
     1081 ctggtgccgc ttgcctccgc tggcagaatc aggtcatggg atcgagtgcc aagaccgagt
     1141 actgcgcgtt caccaaggcc gtggagcacc tgggtgaccg atggagcctg ctgatcgtgc
     1201 gagagctggc gatgttcggg ccacagggct tcaacacgct ggcgcagggc ctgccgggtc
     1261 acgtctcgcg ctcggtgctc gccgagaagc tgcgcaagct cgaggaggtc gggctgctgg
     1321 ctcgtgaccc ccggcggccc tcgggaccgg cgccctactg cctgacgccc gccggcgagc
     1381 agctgatgcc cacgctcaag tcactctggg agtgggcgga gaaatgggtc ccagaggatc
     1441 cgcgcatggc ccagcgcgac ccgaccatca tcctgtggtg gctgacccac cgggtcgacg
     1501 tcgcggccgg accggagcgg caggtcgcgc tcgagatcaa catggaggcc cccggcgccg
     1561 agcgggtgtg gctgctgctg gcgcgcggta ccgagccgac gctgtgcctg gaggacccgc
     1621 tgctggggga agaccgttac gtatacctcg aggcggatgg cgaccgcctc tacccgatcg
     1681 cgcgcggcct ccgttcctgg tcggatgccc tcgcagatgg cagcgtccgc gcctatggcg
     1741 agccgtcgct ggtacaggcg ctgcccggct ggttcgtccc ggccgggagc cagcaccgca
     1801 acggcgaggg ccgtgccgag gtcgcgcccg agccggtgtc cgccctgtcg tcctagtcgc
     1861 tgccccggtc gccggcggac cgtaccgcgc gactggaccc taccggcccc gactggaaaa
     1921 gccggtgtac tccaccgagg cctccctggc tgcctccttc gggtccttcg gcgcgccctt
     1981 tcgcagctcc tcgcccgacg gcagcgccga agacggtacg gagggacgga tcggccgctc
     2041 cccggtgagg aaggcctcga tggcgcgccg ggcgagtgcc gccgagagga gcgtgagcgc
     2101 cgcctcagcc gccttccagc cggcgcgtcc ggcgcccggc ggcccatcga ccatggcacg
     2161 cgccagctcc ttgcgtgcct tcttgttctt gaacgggtcg ttgcgccagc gcagcagctc
     2221 ctcggtgatc gccaaacggg ccttgtcggt cttgggccca agctcgccgc tgacggtgtc
     2281 cacccagtgc tggagtccct tgggcagggc gtcgtaggcc tgctcggcag tggtgggtcg
     2341 catgcggcgg cgcagaaggc gcgccacccg caccggaccg ccggccacca ggaagacggc
     2401 gccggccccc aggccgatga acagcgccgg gttctcgcgg aagcgcgcct tgaagtcgag
     2461 cgcatggcgg acgcgcgcct ccagcgcggc ggcctgccgc tccagctcgg cgcgctgggc
     2521 ggtgacctct agccgcgtct catcaagcgt ttcgcccacg cgatgtcctc cttcacggct
     2581 tcgatcgtct cctccggtgg accgatgcgg atgcgccgga tgcccagcca ggccagcagg
     2641 atggcgagcc cgatgaagac caccaggacg atgagcgcca ccagccaccc gggcagcccg
     2701 gtcagggcgg cgatggccag cacgatgagc atcacgaagg tgatcagcgc cagcagcacg
     2761 atcaccagcg ccacgccgat cagcatcagg ccggacttcg tctcggccag catctggccg
     2821 atctcctgcc ggccgcgggt cacctccagc cgcgcgagct gaatcacccc gctgatcagc
     2881 tgccgcgcct ggccgaagat gctctggcgc ggtgccggct cgccctgcgt catctcacct
     2941 ccttcttgtg gccgtgggca gcaaaggccg cgtcgagcgc ggccaggaat cgattctagc
     3001 aagcaccgcg ccgactcccc tccagccgtg cggcgcttcg acgccgtccg ggcgcctcgg
     3061 ctatcctgtc gcgccaatgg ccaagcgacg tacctccggc atccagcgcc gccgagcgag
     3121 caacgatgcc ctcacccgtc ccctggacgg ccgcggcagc tctcccgcgc tcgatccacg
     3181 catccttatc gtgggtggcg tgctggtggt gggtgccatc gtactggtgg tcgtccttct
     3241 cttcggcgga ggcggagcgg cccctgtggg cacccaggtg ccggatgaag gcgccggtca
     3301 cgtggaacag ggcgagttcc cgacctacca gtcgcgcccg gcgacctccg gcccgcactg
     3361 gaacgtggcg aacctggcgc ccatgaactg gggcgtctac acctcgccgg tccccgagcc
     3421 cggcgttgtg cacaacctgg agcacggcgg catcgtgatc tggtaccagc ccaacgagac
     3481 cagcgcggag gacgtccagg cgctcaccga gttcgtcgag cagcagattc gcggctcccg
     3541 cttcaaggtg atcctgtctc cgtgggccgg cgaggacttc ggcagcccca tcgcggtcac
     3601 cgcctggaac tgggtgatgt acctgaatac cgcggacatc gaccagatcc gggccttcat
     3661 cgacgaccac taccagcagg cgcccgagaa cttcggcggg ccgggtcagc cggcgaccta
     3721 ggaaagcgcg cccctgcgcg actcgctcgg ttgaccgggt gccctccctc cggcatcatc
     3781 tcttcgggcg cgtagctcaa cggcagagca gcggactctt aatccgcggg ttgtgggttc
     3841 gaatccctcc gcgctcgcca gactccctac ttcaccgcga ccacgtccac gtcccccgag
     3901 cggtgcagga cccggaagcc caccccgtga gtctggcgct cgagctccag gtcgaccgat
     3961 gcctctccca cccgcagccc gctgatgctg agccgatcca ggaactccgg caggcgggcg
     4021 tacgagaagc gcacctggtg ggcgggcgca tcgatctcca ttcccaggct ggcctgcagc
     4081 agcatgaaca cggcaccggc cgcccaggcc tgcggtgagc aggcgaccgg gtagcgggtc
     4141 gggccctccc cgtcccggcg ggtgaagccg cagaagagct ccggcaggcg ggcaagatcg
     4201 aagtatcggc tggcgtcgaa gagcgcggtc agcatgcggg ccgcggcgtc gtgccggccg
     4261 tagcgcgcca gtcccatggc caccagcgcg ttgtcgtgcg gccacacgga cccgttgtgg
     4321 taggacatcg ggttgtagcg cgcggcgccc gcggccaccg tcctgacccc ccagccggag
     4381 aagctgctgt cggccatcag cgcctcggcc acccgctccg cccgcgacgg cagcgcgatg
     4441 ccggtcatca gggcgtggcc ggcgttcgag gcgcgcacgc ggcacgctcg tttgtcgccg
     4501 tcgagcgcca gcacgtacgt gcccagctcc tcgtcccaga aggcagcatc gaaccgatcg
     4561 cggagctcgg aggcctggcc ccgcagctgg ctggcccgct cgccatggcc aagcagctca
     4621 gccaggtcgg cggccccgcg cttggcggca taggcgtatc cctgcagctc gcagagggcg
     4681 atcggagcgt cggccagccg gccgtcctcg tggaagatcg agtcgtggga gtccttccat
     4741 ccctgctgca ccaggccatc gggccgatgg cgggcatact ccaggaaccc gtcgccatcg
     4801 gggtcgccgt acgtgtccat ccattcgagg gcacgctcga cgtgcggcca tagccgctcc
     4861 gccgtgacgg cgtcatcggt ctggcgccag tgcgcagccg ccagcatgac gaacagcggc
     4921 gtcgcatcgt gcgagccgaa gtaccggcga aacgggatct cgcccagcgc cgccatctcg
     4981 ccgtttcggg cctcgtgcag gatcttcccc ggctcggcgt ccgcctcggg atccacctgc
     5041 gcggcctggc gcgcagccag gtagccgagt acgccctgcg cgatacgggg agccacccac
     5101 agtgtctgga agccggtcac cagcgcgtct cggccgaacg gcgcgctgaa ccagggaacg
     5161 ccggcgtacg ggtacagccc gtcggatgtt cgtgaggtca gcatggcgac gtccgccacg
     5221 ctgcggtcca gccagtcgtt gaacagctcg tttgagctgg tcaccgatgc ggcgtcccca
     5281 aggacatgcg gcaggtgccc gcgggcgcga tcgaacgcct cgtccaccga tgacagcacc
     5341 gagcgacggc ggctgccggt ctcgcaggcg atcgccacct cgatggtcac cgcgcgggca
     5401 tcgggcatgt gcaggtggaa gctggcctgc ccctcctcca ggaggtccgg tggccgcgag
     5461 aaggtgatcc ggctgcgccg catcaccccg tccaggccgg cgtagcgcag aacgacgacc
     5521 cgttcggaga cctgggtcgt gagccggcgg ccgcgctcgg cgcgctccat gccgcgcacc
     5581 tcgaagatat cggcgaaatc tgcctggaag cgcagcgtca gcggaatgcg cagcggccgc
     5641 cgcgtgaagt tgcgggccac caggcgctcg tacaggacgc catcccacag caccttggtc
     5701 cgcgacaggt gcacgctgct gtgcgggatg cggtccgcgc cgtcgcccac gtccccgttg
     5761 gtcaggtcca cggccaggcg cgagttgtcg cgctgcgcgg tcgagcccag cagcagcggt
     5821 cggcggccgg ccatcagcag ctgcagcgag gagacgtgcc gcgtgccgag gtggtagatg
     5881 ccctcctcgc cgagcccgga ggcctgcacg tcgccgtggc ggtcgaagac ggcgaatgac
     5941 tcggcgtgct tgaggacctg gtcgtgctcc tcgcccgggt cgccgccggc ctggacctcg
     6001 tacgaggggg ccaccagcgg gtcggtgtgg agcaggtcga gcggctttgg ccgttcggca
     6061 cccaccttca gtctggcttt cgtcatcggc tcgcctgaca gtctgccatg tcccggccgg
     6121 cgcgcggcag gcggtgcggt cgacccttcg tgtgtgccgt ccaaatcggt ccgtggtagc
     6181 ctctcgtggc ccgccctcaa ccctccgtgg aggagccgcc gaccgtgccc catgtcccga
     6241 tccagcgcca ccggctgcca aatggcctgc gcgtcatcct gtcgccggac gacctggcgc
     6301 cgatcgtcgc cgtcaacctg tggtatgacg tcggcagcaa gcatgagaag cccgggcgca
     6361 ccggcttcgc gcacctgttc gagcacatga tgttccaggg ctcggagcac gtggccaagg
     6421 gcgagcactt cgcgctcgtg caggcggcgg gcggcacgct gaacgccagc acctggctgg
     6481 accggacgaa ctacttcgag accctgccca gccacgagct ggagctgggc ctgtggctgg
     6541 agtcagaccg gctcgccagc ctgctgccgg ccatgaccca ggagaagctg gacaaccagc
     6601 gcgaggtggt caagaacgag cgccgctggt cggtcgacaa ccagccctac gggacctggg
     6661 acgagaagct ccaggcgctc ctctacccgg agacccatcc ctaccaccac tcgacaatcg
     6721 gttccatgga cgacctgaac gcggccagcc tggacgacgt gcgcgagttc ttcgccacct
     6781 actatgcgcc gaacaacgcg gtgctcaccg tggccggcga cttcgagccg gccaccgccc
     6841 tggccatgat cgagcgtcat ttcggaccga tacccgccaa ccccaacctc ccgccggcac
     6901 ccgacatgag cgtggccgcg ctgctgggcg acgaggtgcg cgaggtggtg ccggaccgcg
     6961 ttccactgcc gcgcgtgtac gtcggcttcc gcatgccggt cttcggcagc agcgagttcg
     7021 aggcgctcag cgtcgccgcg gacgccctgg gagtcgggcg cgcctcgcgg ttgtaccgcg
     7081 tgctggtccg ggagcggcag ctggcccagg acgtcaccgt gttccccttc ccggtggtcg
     7141 gcggcgcctc gatgttcacc atgtgggcca ccgctcgacc aggcgtgccg tccgcggacc
     7201 tcgaggcggc gatgctggcc gagatcgacc atctggcagc cgacgggccg tccgacgcgg
     7261 agcttgagcg ggtgcgcaac ctgcacgcgt cggcggtgga atccagcctg gagcgcatcg
     7321 ccgagcgcgc ggatcggctc tcgatgtaca cctgcctctt cgacgagcct gaccgcatca
     7381 acaccgaggt cagccgctac ctggcggtgg atgccagccg ggtgagggag gcgatggcag
     7441 cggtgctccg tcccgacaac cgcctggtgc tgacctacgt ccccgccgag gcccccatgg
     7501 atccgaccga tgccccggcc gcgaccgagg aggtgacatg accctcacca ccaatccgct
     7561 gcgccccacg ccgggcgccc cgcggcccta ccgcttcccc gagttcgacc ggcgccggct
     7621 ggacaacggg ctgacggtct ggatggtgcc gctgcccgcc gcccacgtgg ccagcgtcca
     7681 cctgctggtg gacgccgggg cggccgccga ggaggaggcc agggccggtg tggcggcgct
     7741 gaccgcgcag ctgctggtga ccggcacccg tcgactggac gcgtcggcct tcgccgaggc
     7801 caccgagcgg ctgggcatcg aggtcagcag cgagtcgagc tgggactcgg cgcgcgcggc
     7861 cttccagtcg ctccccgagc acatcgaggc cggcatggcg ctcctggccg agatggtccg
     7921 cgagccgcgc ctggatccgg ccgagttcga ccggctgcgt gccgagcgcc tggcggacat
     7981 cctgcagtcg cgcgcagatc ccggccgcct ggctgaggag atgttcctgc gccacaccta
     8041 cgacgacacg accccctacc gccgcctttc ggctggcgtg ccggagaccg tctcgaccct
     8101 gagcctggac gacgtgcgcg cccaccacga gaccaactgg cggccgggca acgcccacct
     8161 catcgtggtt ggctggatcg accccgaagc cgtcatgcgc gcggcgcagc gcgccttcgg
     8221 cgactgggaa gggacggccg ccggtcatcg gacgctgcat ccggcgccgg ccggtgaccg
     8281 ccgggtggtg atcgtcgacc gtcccgggtc cgtgcagagc gagctacgga tcggccatat
     8341 cgggatcgaa cggacgtccg accgatactt cccggccatg gtcatggggg cg
//
LOCUS       DATACA010000321         2642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2129192, whole genome shotgun sequence.
ACCESSION   DATACA010000321 DATACA010000000
VERSION     DATACA010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129192"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VHK28_06580"
     CDS             complement(<1..824)
                     /locus_tag="VHK28_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX2141928.1"
                     /translation="MIILQAALTGALVGGLYAVMAVGVSLSWGTLRIINLAHFGMIGL
                     GGYLTLELANRVGMDPLLTVLITAPLLFLLGALLQWLFQRFAVGEFNSLLISFGILIV
                     ILQLISNVWSADFQRMGAEVNPYASGALALGPFAFPMPTLLAFGFALAIVAGAHLALT
                     RTYAGRALRAFAEDRPIAAAFGIDHQRIGILLSGLVGASAAVAGMLFAVGSSLTPSIP
                     FEWFGVVFAIVILGGIGNVIGTLLAGMLISVVSAVVSVVWTPAVAPFVVFSVVIAAL"
     gene            complement(902..2218)
                     /locus_tag="VHK28_06585"
     CDS             complement(902..2218)
                     /locus_tag="VHK28_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2141929.1"
                     /translation="MRKLPIPAALGAVFMAVWLGACTAATTTSPGPSQPAGSDPPPSG
                     EESPGEPSGEPIVVGSTLSLSGIFAATGAIHQIAGEQFVERLNDNGGLLGRPVEWMVL
                     DDESDQAQVGTLYERLISQEQVDLIIGPYATPNILAAMAVAQRHGYVLPQHTAVLAPF
                     MNYECQFPGWSIGPTPNEYVPNMLFDALESLPEPPETIAIVTNQNGSTDFISRGLADD
                     ETDAGALTIAPERGLDLVLELPYPPGTSEWGPIAAEIRDADPDVVMNQGLGVDPVGMI
                     EAMQQLDYEPPIMFSLFPAPGPPLGLGDAAQGMLSVSIFEGTEAALEEAGDEVREIVE
                     TFEANSEEAGLPYTTFETQAAASWHAWEILAQGVEGAGDVDNEAICSYLQENGADLTF
                     SGQVQFDPEVNNFWDSNQGLKQIQGDEWVMVWPEDVATAELQGPGS"
     gene            complement(2556..>2642)
                     /locus_tag="VHK28_06590"
     CDS             complement(2556..>2642)
                     /locus_tag="VHK28_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2141930.1"
                     /translation="GNRSKAAQELGIYRRLLYAKMREYGLGE"
BASE COUNT          422 a          901 c          927 g          392 t
ORIGIN      
        1 agcgcggcga tcacgaccga gaagaccacg aagggagcca ccgccggcgt ccagaccacc
       61 gagaccacgg cggataccac gctgatcagc atgccggcca gcagggtgcc gatcacgttg
      121 ccgatgccac ccaggatgac gatggcgaag accaccccga accactcgaa ggggatggac
      181 ggcgtcagcg aggagcccac cgcgaagagc atgccggcca ccgccgcgct ggctcccacc
      241 aggcccgaga gcaggatgcc gatgcgctgg tggtcgatgc cgaaggcggc cgcgatgggg
      301 cggtcctcgg cgaaggcgcg cagcgcccgc ccggcgtagg tgcgggtcag ggccaggtgg
      361 gcgccggcca cgatggccag cgcgaagccg aaggccagca gcgtgggcat ggggaaggcg
      421 aacgggccca gcgccagggc gccggatgcg tacgggttga cctcggcgcc catgcgctgg
      481 aagtcggcgc tccacacgtt gctgatcagc tgcaggatca cgatcaggat cccgaagctg
      541 atgagcagcg agttgaactc gcccaccgcg aatcgctgga agagccactg caggagcgcc
      601 ccgagcaaaa agagcagcgg ggcggtgatc agcaccgtca gcaacgggtc catgcccacc
      661 cggttggcca gctccagggt gaggtagccg cccagcccga tcatgccgaa gtgggccagg
      721 ttgatgatgc gcagcgtgcc ccacgacagc gacacgccga ccgccatcac ggcgtacagc
      781 ccgccgacca gcgccccggt cagggccgcc tgcaggatga tcacgtcggc gccagcctac
      841 gccgaatgcg ggcgagcggt cccccgtcgc gcgggggacc cgccctccat gcccgccagg
      901 ctcagctgcc gggaccctgg agctcagcgg ttgcgacgtc ctcgggccag accatgaccc
      961 actcgtcgcc ctggatctgc ttgaggccct ggttgctgtc ccagaagttg ttcacctccg
     1021 ggtcgaactg gacctggccg ctgaacgtca ggtcggcacc gttctcctgc aggtagctgc
     1081 agatggcctc gttgtccacg tccccggcgc cctcgacgcc ctgggccagg atctcccagg
     1141 cgtgccagct ggcggccgcc tgcgtctcga aggtcgtata cggcaggccc gcttcctccg
     1201 agttggcctc gaaggtctcc acgatctcgc ggacctcgtc gccggcctcc tcaagcgccg
     1261 cctcggtgcc ctcgaagatg gagacagata gcatcccctg cgccgcgtcg cccagtccca
     1321 gcggtgggcc gggagccggg aagaggctga acatgatggg cggctcgtag tcgagctgct
     1381 gcatggcctc gatcatcccc accgggtcca cgcccaggcc ctggttcatg accacgtccg
     1441 ggtccgcgtc gcggatctcg gcggcgatgg gcccccactc gctggtgccc ggcgggtagg
     1501 ggagctccag caccaggtcc agccctcgct cgggggcgat ggtcagggcg ccggcatcgg
     1561 tctcgtcgtc cgccaggccg cgggagatga agtcggtgga gccgttctgg ttggtgacga
     1621 tggcgatcgt ctcgggcggc tccggcaggc tctcgagggc gtcgaacagc atgttcggca
     1681 cgtactcgtt cggcgtggga ccgatggacc agcccgggaa ctggcactcg tagttcatga
     1741 acggcgccag cacggcggtg tgctggggca gcacgtagcc gtgccgctgc gccacggcca
     1801 tggcggccag gatgttcggg gtggcatatg gaccgatgat gaggtccacc tgctcctgcg
     1861 agatgagccg ctcgtagagg gtgcccacct gcgcctggtc tgactcgtcg tccaggacca
     1921 tccactcgac cggccggccc agcaggccgc cgttgtcgtt gagtctctcc acgaactgct
     1981 cgccggcgat ctggtggatg gcgccggtcg ccgcgaagat tccggagagg gacagcgtgg
     2041 acccgaccac gatcggctcg ccggatggct ccccggggga ctcctcgccg ctgggcggtg
     2101 ggtcgctgcc tgccggttgg ctgggtcccg ggctggtggt ggtggcggcc gtgcaggccc
     2161 ccagccagac tgccatgaag acggcgccca acgccgcggg aatgggtagc ttcctcacct
     2221 tgcactcctc cagggtgatg ctctcggccc cgctcggagg tcgtgccgga agtgtagcga
     2281 cgctgcacaa cccgttgcaa tatcactgtc cgcgtccagt gcagttacag gggcgagccg
     2341 caccggtacg gcagtgcttc caagaacctg ccggcctggc ccagcttcgt tgggaaatgt
     2401 cgtacgggtg cggcgtaccc atcacgttcc ggcgctaagc gcccaccttc gtcggcatta
     2461 ggccgcaccg gtgcggcatt tccggaggat gtggccgcca cacgagaaca tcgtggagaa
     2521 gctgccgcgc gggtgcggca cccagccgaa gggggctact ccccgaggcc gtactcgcgc
     2581 atcttggcgt agagcaggcg gcggtagatg cccagctcct gggcggcctt ggagcggttg
     2641 cc
//
LOCUS       DATACA010000322         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_202620, whole genome shotgun sequence.
ACCESSION   DATACA010000322 DATACA010000000
VERSION     DATACA010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202620"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VHK28_06595"
     CDS             complement(<1..333)
                     /locus_tag="VHK28_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141931.1"
                     /translation="MQQRFATIVRFVAMLGLLAMVACGGGEEPAAEPSTAEPDEPSAA
                     ASQPATDAAPAQAGGAEPTAAADEQGDTGFEDVMAAVEGLDGQERIDELVALAQDEGP
                     VQIYSTMNS"
     gene            complement(386..1249)
                     /locus_tag="VHK28_06600"
     CDS             complement(386..1249)
                     /locus_tag="VHK28_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2141932.1"
                     /translation="MAITAIDVHIHIHDARARELRGPKARENQEAMARYFGREAEAIS
                     IDEQAEDYRSRNMMAVLMNGTDETISGIPAIPNDFVAEAVQKHPDVFLGMGIVDPWQG
                     ELARQEIRRCKDMGLVGLGEFNPARQHFYPNDPRFYPLWEECQELGMVLLFHSGYAAA
                     GSGQPGGRGVKLKYCQPIHLDDLAADFPDLKIISAHPSWPWTGESLAIARHKPNFYID
                     LSGWAPKYFPAEVVQQVNSLLQNKALFGSDAPSLPLDRWLSDFEKLDLKPEVRQKIML
                     DNAKKLFNLDV"
     gene            complement(1338..>2070)
                     /locus_tag="VHK28_06605"
     CDS             complement(1338..>2070)
                     /locus_tag="VHK28_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX2141933.1"
                     /translation="LHLLPAAVPTLDDPPPRAEGHRMQAPETLDHGAVVASIEGPVAH
                     VELNRDEKRNAVNAAMAQAFLEVDDELAARGVTASVLTARGSLFCAGVDLKEPRLPGQ
                     PAPFVPMLERLRGSDRLWVAAVQGGAIGAGVALALACPLVLMSADAWLWLPEVSKTGR
                     APLGVLRWVGPVIGPRGAFDLAFSERKVGAEEAASRGWITAAHAPEDLLPAARALAGR
                     AAGAEADSIREAVALWSVLSAPVSH"
BASE COUNT          322 a          758 c          669 g          321 t
ORIGIN      
        1 ggagttcatc gtggagtaga tctggaccgg gccctcgtcc tgcgccaagg cgaccagctc
       61 gtcgatgcgc tcctggccgt ccagcccctc gacggcggcc atgacgtctt cgaagcccgt
      121 gtcgccctgc tcgtcggcgg ctgcggtcgg ctccgccccg cccgcctgcg ccggtgcagc
      181 gtcggtcgcc ggctggctgg cggccgccga cggctcgtcg ggctcggcgg tggagggctc
      241 cgcggcgggc tcctcaccgc cgccacaggc gaccatcgca agcagaccca gcattgcgac
      301 gaagcggacg atggtcgcga agcgctgctg catgggttct ccgagatcgg aatcgacgtg
      361 tggcctcacg tgccgagccg gcggctcaga cgtcgaggtt gaacagcttc ttggcgttgt
      421 ccagcatgat cttctgccgc acctccggct tgaggtccag cttctcgaag tccgacaacc
      481 accggtccaa cggcaacgac ggcgcatccg acccgaacag cgccttgttc tgcaacaggc
      541 tgttgacctg ctgcaccacc tcagccggga agtacttcgg cgcccagccc gacagatcga
      601 tgtagaagtt cggcttgtgc cgcgcgatcg ccaggctctc acccgtccac ggccacgacg
      661 gatgcgcact gatgatcttc agatccggga agtccgccgc cagatcatcc agatggatcg
      721 gctggcagta cttcagcttc accccccgac cacccggctg acccgacccc gccgccgcat
      781 agccggagtg gaacaacagc accatgccca gctcctggca ctcctcccac aacgggtaga
      841 accgcggatc gttgggatag aagtgctgac gcgccgggtt gaactcgccc aaccccacca
      901 accccatgtc cttgcaccgg cggatctcct gacgcgccag ctccccctgc cagggatcga
      961 cgatccccat ccccaagaac acatccggat gcttctggac cgcctcagcc acgaaatcgt
     1021 tggggatcgc cgggatcccg gagatggtct catcggtgcc gttcatcaac accgccatca
     1081 tgttgcgcga gcggtagtcc tcagcctgct catcgatgga gatcgcctcc gcctcccggc
     1141 cgaagtagcg cgccatcgcc tcctggttct cccgcgcctt ggggccccgc agctcgcggg
     1201 cccgcgcatc atggatgtgg atgtggacat cgatcgcggt gatcgccatg cgtgcatctc
     1261 ctcgcagtcg tgcggcggtt gacagatgga gctcgcgggt cgcctcgtgc cggcggagcg
     1321 gcgcgaaggc gtggtggcta gtgcgagaca ggagccgaca gcaccgacca cagcgccacc
     1381 gcctcccgga tggagtcggc ctccgcaccg gcggcacgtc ctgcgagcgc ccgggctgcg
     1441 ggcagcaggt cctccggcgc gtgcgcggcg gtgatccacc ccctggaggc ggcttcctcg
     1501 gcgcccacct tgcgctcgct gaaagccagg tcgaacgcgc cccgggggcc gatcaccggg
     1561 cccacccagc ggagcacgcc cagcggcgca cgaccggtct tgctgacctc gggcagccac
     1621 agccaggcgt cggcagacat cagcacgagc gggcacgcca gcgcaagcgc cacacccgcg
     1681 ccgatggccc cgccctgcac tgcggcgacc cacagccgat cgctgcctcg caggcgctcg
     1741 agcatgggca cgaacggcgc tggttgcccg ggcaggcgtg gttccttgag gtcgacgccc
     1801 gcgcagaaca ggctgccgcg agcggtcagg acgctggcgg tgacgcctcg agccgccagc
     1861 tcgtcgtcga cctccaggaa cgcctgcgcc atggcggcgt tgaccgcgtt gcgcttctcg
     1921 tcccggttga gctccacatg cgccaccgga ccctcgatgg aagcgaccac tgccccgtgg
     1981 tcgagggtct cgggtgcctg catgcggtgt ccttctgctc gtggaggcgg atcgtccagc
     2041 gtcggaacgg cagctggcag caggtggagc
//
LOCUS       DATACA010000323         4276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2771737, whole genome shotgun sequence.
ACCESSION   DATACA010000323 DATACA010000000
VERSION     DATACA010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2771737"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VHK28_06610"
     CDS             complement(<1..987)
                     /locus_tag="VHK28_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2141934.1"
                     /translation="MALKKAKSPAAGEFRRPIFDEILERAHEPRQFIQVLAGPRQVGK
                     TTVARQVMASIGMPGHFASADAPDPEDIGWLYTQWAIGRQAARAGGRAGGILVLDEIQ
                     KIRDWSDVVKELWDEDSATGLPLRVMLLGSAPLLVRQGLSDSLAGRFEVVRVTHWTFA
                     EMRDAFGWDLDTYLFYGGYPGAARLVKRHDRWEEYILDSIVETNLSRDILLLNRVEKP
                     ALLRQLFRLACSYSGQVLSYTKMLGTLTDAGNTVTLAHYLELLGGAGMVAGIGKYSGS
                     AVRRRASSPKLLALNTALISATARTSMAKARADHEFWSRLVETAVGAHLWAHA"
     gene            complement(1060..2232)
                     /locus_tag="VHK28_06615"
     CDS             complement(1060..2232)
                     /locus_tag="VHK28_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEX2141935.1"
                     /translation="MIEPAPIRVAPEAATSRLRRAYGFEEVALVPGAITADPAEVDLS
                     VEIGGFRLEVPFLAAAMDAVADADFAVRMSRHGGLAFINLEGLYTRYEDAGPIIERVA
                     EAASGRAAAHVLAEAYRSPVRDELITALITRIKAQGALAGVATTPAAAWHHAEVAAEA
                     GADLFLVQSQVSSARHISTSTRVLSLSDLTGGLRLPVMVGNTVSGEAAYRLMHQGAAA
                     ILVGVGPGAACTTREVLGIGVPQVTATLDVSAARDRYLDETGRYVPVITDGGMKTGGD
                     IAKAIASGADAVMLGSPLAAAVEAPGRGFSWGMASPSPTLPRGTRIRIGERLALEQIL
                     FGPAETTHGTQNLVGALRQSMAALGARTIREMHSVEMVIAPAIATEGKSWQLAGRE"
     gene            complement(2414..3571)
                     /locus_tag="VHK28_06620"
     CDS             complement(2414..3571)
                     /locus_tag="VHK28_06620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2141936.1"
                     /translation="MAAPFSQAVIAVVWDFDKTLIAGNMQAPLFRRYGVDERAFWNEV
                     DQLRSFYLSHGAHRVNDDVLYLNHILEYVRAGTFRGLSNAVLRDLGADLTFLPGMPEF
                     LPTLRALVEDEPLFQRADISVEHYIVSTGLRPMIEGSAIRPYVDDVWACEFIEAHHPP
                     GFDRPEQRQLFPKREITALGYQIDNTSKTRALFEINKGSNKRPEIHVNAAIPRELRRV
                     PFENMIYVADGPSDVPAWSLVRGNGGMTFGVYQRRSLGQFEQISSLQRDGRIHAFAEA
                     DYRPHSTAHLWLTTEVRRIAERIVEERDVALRRAVRAAPRHVVDVPATTLTDGPMEEP
                     ADVPPQATGEPSPPRADLIRDAVRRSMNEEDGSDAQAGSAGETPVQDRLPF"
     gene            complement(3638..>4276)
                     /locus_tag="VHK28_06625"
     CDS             complement(3638..>4276)
                     /locus_tag="VHK28_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2141937.1"
                     /translation="KMAGTLDVISGGRFDLGIGAGWKRDEWEAYGYGFPETRQRLAQL
                     RDGLEVITRMLAGGAQDHATFEGEHASVRNAINWPKGLQAPRIPIMVGGNGPEVTWRL
                     AARFADELNLDGLSPAELRDALPVIRSRCEEIGRDPATLRLSLHAWWGEGDWSRAGDG
                     RIAYLMQLADLGLHRVIGLLQASAASDEALIALAEDASAAGVELSLETAAAR"
BASE COUNT          632 a         1492 c         1505 g          647 t
ORIGIN      
        1 ggcgtgggcc cacaggtgcg cgccgaccgc ggtctcgacc aggcggctcc agaactcgtg
       61 gtcggcccgg gccttggcca tgctggtgcg cgccgtggcg gagatgagcg cggtgttgag
      121 cgccagcagc ttgggcgacg aggcgcggcg gcgcaccgcc gagccggagt acttcccgat
      181 gccggccacc atcccggcac cacccagcag ctccaggtag tgggccaggg tgacggtgtt
      241 gcccgcatcg gtcagggtgc cgagcatctt ggtgtagctc aggacctggc cggagtagga
      301 gcaggccagc cggaagagct ggcgcaggag ggcgggcttc tcgacccggt tgaggagcag
      361 gatatcgcgc gacaggttgg tctcgacgat cgagtccagg atgtactcct cccaccggtc
      421 atggcgcttg accagccgcg cggcgcccgg gtagccgccg tagaagaggt aggtgtccag
      481 gtcccagccg aaggcgtcgc gcatctcggc gaacgtccag tgcgtgaccc ggaccacctc
      541 gaagcgcccg gcgagcgagt cggagagtcc ctggcgcacc agcagcggcg ccgagccgag
      601 cagcatgacg cgcaggggca gcccggtggc ggagtcctcg tcccacagct ccttgaccac
      661 gtccgaccaa tcgcggatct tctggatctc gtccaagacc aggatccccc cggcacggcc
      721 accggcgcgg gcagcctggc gaccgatggc ccactgggtg tagagccaac cgatgtcctc
      781 cgggtcgggc gcgtcggccg atgcgaaatg cccgggcatg ccgatggacg ccatcacctg
      841 gcgcgcgacg gtcgtcttcc caacctggcg aggcccggcc agtacctgga tgaactgccg
      901 cggctcgtgc gcacgctcca ggatctcgtc gaagatcggc cgtcgaaact cgcctgcagc
      961 cggcgatttc gccttcttga gcgccactac tcattctcct gagctcaatt actcagtgat
     1021 ttgagtaatt ttactcactg gtgaggtcgc gtgaaggtgc tattcccgtc ccgccagctg
     1081 ccatgacttg ccctcggtgg cgatggcggg ggcgatcacc atctcgacgg agtgcatctc
     1141 gcggatggtg cgggcaccga gcgcggccat cgactggcgc agcgccccga ccaggttctg
     1201 cgtcccgtgg gtcgtctccg ccgggccgaa gaggatctgc tccagcgcca gccgctcgcc
     1261 gatgcgaatg cgcgtcccgc gcggcagggt gggcgatggc gacgccatcc cccagctgaa
     1321 gccacgtccc ggggcctcga ccgcggcggc caacggcgag ccgagcatga ccgcgtcggc
     1381 acctgacgcg atcgccttgg cgatgtctcc gccggtcttc atgcctccgt cggtgatgac
     1441 aggaacgtag cgccccgtct cgtccaggta gcggtctcga gcggcggaga cgtccagcgt
     1501 ggcggtgacc tgcggcacac cgatgcccag cacctcgcgc gtggtgcagg cggccccggg
     1561 ccccacgccg accaggatgg cggcggcgcc ctggtgcatg agccggtagg cggcctcgcc
     1621 actgaccgtg ttgccgacca tcaccggaag ccgcagccca ccggtcaggt cggacagcga
     1681 gagcacacgc gtggaggtgg agatgtgccg cgcgctggaa acctgggact gcaccaggaa
     1741 caggtcggcg ccggcctcgg cggccacctc ggcgtggtgc caggccgcgg cgggcgtggt
     1801 cgccaccccg gccagcgcgc cctgcgcctt gatgcgggtg atcagcgcgg tgatcagctc
     1861 gtcgcgaacc ggagaccgat atgcctcggc cagcacgtgc gcggctgcgc gaccggaggc
     1921 tgcctcagcg acccgctcga tgatcggccc ggcgtcctcg taccgcgtgt acaggccctc
     1981 gaggttgatg aaagccagcc cgccgtggcg gctcatccgc accgcgaaat cggcatcggc
     2041 caccgcgtcc atggcggctg ccaggaacgg cacctccagg cggaagccgc cgatctcgac
     2101 ggagagatcg acctcggccg ggtcggcggt gatcgccccc ggcaccagcg cgacttcctc
     2161 gaagccatac gcccggcgca ggcggctggt ggctgcctcg ggcgcgaccc gaatcggggc
     2221 tggctcgatc aacggaacct cctcgggtat gaaaacgccc gcgcggcagg gcggcgcggg
     2281 cctggggtcg aatgggtggc gctgacggct ctgatccggt gcattcggcg cgggcgtgcg
     2341 gctttggcgg agtatacgcc gctgtcctca gcgcggaccg ctcgtttggg cgtccctccc
     2401 ggagagaggc accctagaag ggcagccgat cctggacggg cgtctccccg gccgatccag
     2461 cctgagcgtc cgatccatcc tcctcattca tcgagcgccg gaccgcgtcg cggatcaggt
     2521 cggcccgcgg tggcgagggt tcccccgtcg cctgcggcgg gacgtcggcc ggctcctcca
     2581 tcggtccatc cgtgagggtt gtggccggca cgtccacgac atgcctgggc gccgcgcgca
     2641 ccgcgcgtcg cagggcgacg tctcgctcct ccacgatgcg ctcggcgatg cgacgcactt
     2701 cggtggtcaa ccacaggtgg gccgtcgaat ggggtcggta gtccgcctcg gcgaaggcgt
     2761 ggatgcgccc gtcgcgctgc aggctgctga tctgctcgaa ctgcccgagg gagcgtcgct
     2821 ggtagacgcc gaaggtcatg ccgccgttgc cgcgcaccag gctccaggcc gggacgtccg
     2881 agggcccgtc ggcgacgtag atcatgttct cgaagggcac ccgccgcagc tcgcgcggga
     2941 tggcggcgtt gacgtggatc tcgggacgct tgttcgatcc cttgttgatc tcgaacagcg
     3001 ctcgcgtctt cgacgtgttg tcgatctggt agcccagcgc ggtgatctcg cgcttcggga
     3061 agagctgccg ctgctcgggt cggtcgaagc cgggagggtg gtgcgcctcg atgaactcgc
     3121 aggcccacac gtcgtccacg taggggcgga tggcgctgcc ctcgatcatg ggccgcaggc
     3181 ctgtgctgac gatgtagtgc tccacgctga tgtcggctcg ctggaagagc ggctcgtcct
     3241 cgaccagggc gcgcagcgtc ggcaggaact cgggcatccc cggcaggaag gtgagatcgg
     3301 cacccaggtc gcgcaggacg gcgttgctga ggccacggaa cgtcccggcg cggacgtact
     3361 ccaggatgtg gttcaggtac aggacgtcgt cgttgacccg gtgcgccccg tgcgaaaggt
     3421 agaaggagcg cagctggtcg acctcgttcc agaacgcgcg ctcgtcgacg ccgtagcgcc
     3481 ggaacagcgg cgcctgcatg ttcccggcga tcagcgtctt gtcgaagtcc cagacgacgg
     3541 cgatcaccgc ctggctgaag ggagcggcca taggaagccc gagtctagac cggtgcgtca
     3601 acgcacccct atggaccgat ggtcccggtc gacccgctca gcgggctgcc gccgtctcga
     3661 gggagagctc cactccggcc gcgctggcat cctcggccag cgcgatcagc gcctcgtccg
     3721 acgcggcgct ggcctgcagc agcccgatga cgcggtgcag gcccaggtcc gccagctgca
     3781 tcaggtacgc gatgcgcccg tcgccagccc gcgaccagtc gccctcgccc caccaggcat
     3841 ggaggctgag gcgcagcgtc gccggatcac ggccgatctc ctcgcagcgc gatcggatga
     3901 ccggcagggc atcccgcagc tcggccggcg acagaccgtc caggttcagc tcgtccgcga
     3961 agcgcgccgc cagccgccag gtcacctccg gcccgttgcc gccgaccatg atcgggatgc
     4021 gcggcgcctg caggcccttc ggccagttga tcgcgttccg gacgctggcg tgctccccct
     4081 cgaaggtggc gtgatcctgg gcgccgccgg caagcatgcg ggtgatcacc tccaggccgt
     4141 cccgcagctg cgccagccgc tggcgggtct ccgggaagcc gtagccgtag gcctcccact
     4201 catcgcgctt ccatccggcg ccaatgccga ggtcgaagcg tccaccgctg atcacgtcca
     4261 gggtgccggc catctt
//
LOCUS       DATACA010000324         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_603379, whole genome shotgun sequence.
ACCESSION   DATACA010000324 DATACA010000000
VERSION     DATACA010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603379"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /gene="pheT"
                     /locus_tag="VHK28_06630"
     CDS             <1..1887
                     /gene="pheT"
                     /locus_tag="VHK28_06630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2141938.1"
                     /translation="AFTGGRLRPPDASVHEDGELRTADLVSVEIADAAGWFEGLANGP
                     SPDWMQRRLVAAGMRPISAVVDVTNYVMHELGQPMHAYDADAVPQGQIVVRRGRPRER
                     LETIDHEERTLDERMLVIADRERAIGLAGIMGGASTEVTDRTTRVILESAVFHGPTIR
                     NTARRLGLRSEASMRHEKGIGHELPRYAADRAAQLIAQITGARVAAGIVDNDPEPKAP
                     RVIAVDLARTERLLGVELDAGKVGEWLDPLGFDVAAGSGRTLEVTVPSFRLDVVAAED
                     VAEEIARAHGYDRIPGRLPTPTLPHWRADPSEPRHAVRRALSALGLTEVVSHALTGPD
                     DRGRTGFDPAAEGLIRIANPLSEAHSQLRATMYPSLLRALSENVRQRRGSAWIFEVGK
                     VYWHDPEASGERYRYAETAGTGRWEQWHVGIALLGAGAPRYPGEIPRPADVADLKGII
                     EGLHAALGAPAPAYRAERPEDLHPHLHPGRAGRLVDAAGEDYGSVGELHPRVAAAWDL
                     PGRPVVSAISLARLLALIPESAALRPVPTAQPIDRDLAVIVDDAAPVGELLRLVRLNA
                     GSMLVEARIFDVYRGEQVGAGRVSYALALRFQPESAGDEKGVERALNKVRGSLRHHLG
                     AEIR"
BASE COUNT          270 a          710 c          755 g          275 t
ORIGIN      
        1 gccttcaccg gtggccggct gcggccgccg gacgcatcgg tccatgagga tggtgagctg
       61 cgaaccgccg atctggtcag cgtggagatc gccgacgccg cgggctggtt cgagggcttg
      121 gccaacgggc cgtcgccgga ctggatgcag cgtcggctgg tggccgccgg catgcggccg
      181 atttcggcgg tggtggacgt caccaactac gtcatgcacg agcttgggca gccgatgcac
      241 gcctacgacg ccgacgccgt gccgcagggg cagatcgtgg tgcggcgtgg gcggccgagg
      301 gagcgcctgg aaaccatcga ccacgaggag cggacgctcg acgagcgcat gctggtcatc
      361 gccgaccggg agcgcgccat cggactggct ggcatcatgg gaggtgcctc caccgaggtc
      421 accgaccgca cgacccgggt catcctggag tcggcggtct tccacgggcc gaccatccgc
      481 aacacggcca ggcgcctggg gctgcggtcg gaggcgagca tgcggcacga gaaggggatc
      541 ggccatgagc tgccgcgcta cgccgcggac cgtgccgcgc agctgatcgc gcagatcacc
      601 ggtgcacggg tggcggccgg catcgtggac aacgaccccg agccgaaggc accccgggtg
      661 atcgcggtgg acctggcccg caccgagcgc ctgctgggcg tggagctcga cgccgggaag
      721 gtgggggagt ggctggaccc gctgggcttc gacgtcgcgg ccggcagcgg ccgcacgctg
      781 gaggtcaccg tccccagctt ccggctggac gtggtggccg cggaggacgt ggccgaggag
      841 atcgcgcgtg cccatggcta cgatcgcatc ccgggacgac tgccgacccc caccctgccc
      901 cattggcgag cggatccgtc cgagccgcgg cacgcggtcc ggcgcgccct gtccgctctc
      961 gggctcacgg aggtggtcag ccacgcgctc accggtccgg acgatcgcgg caggaccggc
     1021 ttcgacccgg cggcagaggg cctgattcgg atcgccaacc cgctgtccga ggcgcattcg
     1081 cagctgcgag ccacgatgta cccgtccctc ctgcgcgcgc tgtccgagaa cgtgcggcag
     1141 cgtcgcggct cggcctggat cttcgaggtg ggcaaggtct actggcacga tccggaggcc
     1201 agcggcgagc gctaccggta cgccgagacg gccgggaccg gacggtggga gcagtggcac
     1261 gtcggtatcg cgctcctggg cgctggcgcg ccgcgctacc ccggagagat tccgcgcccc
     1321 gcggacgtcg ctgacctgaa gggcatcatc gagggcctgc acgccgcgct gggcgcgccc
     1381 gcgcccgcgt atcgagccga gcgccccgag gacctgcacc cccacctgca ccccggtcgc
     1441 gcgggacgat tggtcgatgc cgcgggcgag gactacggca gcgtgggcga gctccatccg
     1501 cgggtcgccg cggcgtggga tctcccggga cgcccggtgg tctcggccat ctcgctggcg
     1561 cgcctgctgg ccctgatccc cgagagcgcg gcgcttcgcc cggtcccgac cgcacaaccc
     1621 atcgaccgcg acctggcggt gatcgtcgac gacgcggcgc cggtcggcga gctgctgcgg
     1681 ctggtgcgtc tgaacgctgg gtctatgctg gtggaggcca ggatcttcga cgtctaccga
     1741 ggcgagcagg tgggcgccgg tcgcgtctcc tacgccctgg cgctccgctt ccagccagag
     1801 tcggcgggcg acgagaaggg cgtggagcgg gcactgaaca aggtgcgcgg ctcccttcgc
     1861 catcacctgg gcgcggagat ccgctaggag ccgattccgg cggcgcgccc gcacgcttgg
     1921 tcgccgcggg ttgtccgtgc ccgacgctgg ctgctaccct tcgcgcgccc gagccccggt
     1981 tcctccggcc ggatcgctcc gagcctgatc
//
LOCUS       DATACA010000325         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_442919, whole genome shotgun sequence.
ACCESSION   DATACA010000325 DATACA010000000
VERSION     DATACA010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442919"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VHK28_06635"
     CDS             <1..607
                     /locus_tag="VHK28_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141939.1"
                     /translation="LLLAFLGGLVAYVLVAAPLRLDPLRYADQLYEIASYKPLISAGA
                     PNPWGVLFGYSAEDGPLLWIGLVLLACGLGFALLPLRRGRDLGTLLAVGAFIVLAFYF
                     LPTRVHERYLFPALAVLAPFAAVSGRMLATYVVLSLAFAASLLAALARINPPSVAQPL
                     RELLLTDAAPWMMGVALAGAALATIWLLRPSATDRVSAPRG"
     gene            complement(588..2804)
                     /locus_tag="VHK28_06640"
     CDS             complement(588..2804)
                     /locus_tag="VHK28_06640"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX2141940.1"
                     /translation="MDLARLRAAPAAVVQRISRGQAAAEPTHTIEDVVEEARRHRPSI
                     NVGLLERAHEVAAMAHEGQLRASGEPYISHPVEVAYYLATLQMDVETLAAALLHDVPE
                     DTELTLADIEKRFGREVGKLVDGVTKLSKFGSARSMEEQQAENIRKMFMAMAEDVRVV
                     IIKLADRLHNMRTLHFLPAEKQQRIARQSMEIYAPLAHRLGMWQIKWELEDLSFKYTE
                     PVKYRQLVEMLADRRKARESFINKSIQILRKELGKVGISADISGRPKHLYSIYKKMER
                     KGAEFNEIYDLHAIRLLVEDVKDCYAALGVVHSIWRPIPGQFDDYIAMPKANMYQSLH
                     TAVIGPDAKPLEVQIRTGRMHDVAEAGIAAHWRYKEGSRGDRRYDEKLAWVRQLIDWQ
                     REVADATEFVEGLKLEVFQDQVFVFTPKGDVKDLPAGATPLDFAYRIHTDVGHRTIGA
                     KVNNRLVPLDYKLRNGDIVEIITTKAAHGPSRDWLNIVQTNAAREKIRQWFKRQQRDE
                     NISQGKELLDRELRRLAHETLASVDLSRLEQIAQQAHYREVDDFFAAIGYGAVSPASV
                     VSKLEIHDDAAITLPEVAPPTAVPSATGVRVKGVGDLLVRFAKCCSPIPGDPITGYVT
                     RGKGVTVHRANCPSVLSERDIERLIDVEWESADQQTYPITVRIEAVDRAGLLNEITNV
                     VAENKVNIVAASIGTQPDGTANITATLKVTSLQQLSKVLTRIERIRDITSVTREAR"
     gene            complement(3051..4100)
                     /locus_tag="VHK28_06645"
     CDS             complement(3051..4100)
                     /locus_tag="VHK28_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEX2141941.1"
                     /translation="MPPIVRRADDTDRPAWDEFLASRPEADLLQAWAWGECTALAGEP
                     AYRLLVEDAGRVRGVAQALLRPAGFGRQVAYVPHGPVWDRSAPDADRVFAWLIHGLRT
                     LARKERALVIKMDPRAELGQEFDFTHLADEHDLRPAPDLQAPTTRIVDLLDGGEQLEA
                     SWHADARRLMRRAEREGTEVSIGRQADEDEVAILHDLLSRAAERAEFRVRSPQFLTRL
                     ATELAASGGWYLGIGRVEGTPIATMAFPRIGDRAYYLYGALLRDERYKHNYGSHAVMA
                     GMLKTLAVDGCRQVDMCGVVEPDDPEADPAWKGFSDFKRTFGGTPLRHPGLFDLVTDP
                     FWYRLRAARERVLRR"
     gene            4143..5165
                     /locus_tag="VHK28_06650"
     CDS             4143..5165
                     /locus_tag="VHK28_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2141942.1"
                     /translation="MSSEIDLPWQRPEWRDEALAWIREQVARLGWRVTGSIEQPHVIW
                     WSSVFRVPTSDGVVWFKAVQPDGAFEARLTPLLAADWPQHTAHILATDAERGWTLARD
                     AGARMRELDDGRAVVEHWERLLPRYAEVQLGMADRRDELLRLGVPDRGLKSLPHELRA
                     ALDEPRVLLIGQEDGLDEADRDALVAGLEEFSELCATLAEMGIPESIQHDDLHDGNAF
                     LRDGEYVFFDWGDACVSHPFHTLVVTLRALAYRQRWEPGGPEVMRLLSAYLEPWTRLV
                     PSEQVLAAADMARRTGTIQRAMAWRDYTAVMPPAVAAENVDSVPYGLRMYLLNAPWGS
                     WDDGTF"
     gene            complement(5192..>5641)
                     /locus_tag="VHK28_06655"
     CDS             complement(5192..>5641)
                     /locus_tag="VHK28_06655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141943.1"
                     /translation="FVARAKRYYERVWGLFAPRGHARLAFAVHDGRRVATLFHFTCGD
                     RAAETFGGMTDEGADSRANYLLKWQAILGFKADGFAVYDMWGLATGGIAQFKEGFGGR
                     EVEYVGARDLPLRPAEDAAMRLLLPAYGIAQRTRLRLMGRRLAGSDD"
BASE COUNT          774 a         1907 c         2007 g          953 t
ORIGIN      
        1 cctgctgctc gcgttcctcg gcgggctggt ggcctacgtc ctggtcgcag ccccgctgcg
       61 actcgacccg ctgcgctacg cggaccagct ctacgagatc gccagctaca agccgctgat
      121 ctcggccggc gccccgaacc cgtggggcgt gctgttcggc tacagcgccg aggacgggcc
      181 cctgctgtgg atcggcttgg tgctcctggc ctgcggcctg ggttttgcgc tgctgccgtt
      241 gcgacggggg cgggacctcg gcacgctgct ggcggtgggc gccttcatcg tgctcgcctt
      301 ctacttcctg ccgacgcgcg tgcacgagcg ctacctgttc ccggcgctcg ccgtcctggc
      361 ccccttcgcc gccgtgtcag gccggatgct ggcgacctac gtggtgctgt cgctcgcctt
      421 cgcggcctcg ctgctggccg ccctggcgcg tatcaacccg ccgtccgtgg cccaaccgct
      481 gcgcgagctg ctgctgaccg acgctgcgcc gtggatgatg ggggtcgctt tggccggggc
      541 ggcgcttgcc accatctggc tcctgcgccc ttcggcgacg gaccgcgtca gcgcgcctcg
      601 cgggtgaccg aggtgatgtc gcggatgcgc tcgatccggg tcaggacctt ggagagctgc
      661 tggagcgagg tgaccttcag ggtggccgtg atgttggccg tcccgtccgg ctgggtgccg
      721 atggaggcgg ccacgatgtt caccttgttc tcggcgacca cgttggtgat ctcgttcagc
      781 agcccggcgc ggtcgacggc ctcgatgcgc acggtgatcg ggtaggtctg ctgatccgcc
      841 gactcccact cgacgtcgat cagccgctcg atgtcgcgct cggagagcac cgacgggcag
      901 ttcgctcgat gcactgtcac tcccttgccg cgtgtcacgt agccggtgat cgggtcaccc
      961 gggatcgggg aacagcactt cgcgaatcgc accagcaggt cgcccacgcc cttgacccgc
     1021 acgccggtgg ccgagggaac cgcggtcggc ggcgcgacct cggggagggt gatggccgcg
     1081 tcgtcgtgga tctccagctt ggagacgacc gacgccggcg atacggcgcc atagccgatg
     1141 gcggcgaaga agtcgtccac ctcccgataa tgcgcctgct gcgcgatctg ctccagccgg
     1201 ctgaggtcga ccgatgccag cgtctcgtgc gccagccgcc tcagctcgcg gtccagcagc
     1261 tccttgccct ggctgatgtt ctcgtcgcgc tgctggcgct tgaaccattg ccggatcttc
     1321 tcgcgggcgg cgttggtctg gacgatgttc agccagtcgc gcgacggccc gtgcgcggcc
     1381 ttggtggtga tgatctcgac gatgtcaccg ttgcgcagct tgtagtcgag cggcaccaat
     1441 cggttgttga ccttggcgcc gatggtgcgg tgaccgacat cggtgtggat gcggtaggcg
     1501 aagtcgagcg gggtggcgcc ggccggcagg tccttgacgt ctccctttgg ggtgaagacg
     1561 aagacctgat cctggaagac ctccagcttg aggccctcca cgaactcggt ggcgtcggcc
     1621 acctctcgct gccagtcgat cagctgccgg acccaggcca gcttctcgtc gtagcggcga
     1681 tcgccgcgtg agccttcctt gtatcgccag tgggcggcga tgcccgcctc cgccacgtca
     1741 tgcatgcggc cggtgcggat ctggacctca agcggcttgg catccggccc gatgaccgca
     1801 gtgtgcaggc tctggtacat gttggccttg ggcatggcga tgtagtcgtc gaactgcccg
     1861 ggtatcggtc tccagatgga gtgcaccacg cccagcgcgg cgtagcagtc cttgacgtcc
     1921 tcgaccagca gccggatggc atgcaggtcg tagatctcgt tgaactcggc gcccttgcgc
     1981 tccatcttct tgtagatgga gtacagatgc ttggggcggc cggagatgtc cgcgctgatg
     2041 ccgaccttgc ccagctcctt gcgcaggatc tggatgctct tgttgatgaa cgactctcgg
     2101 gccttgcgcc ggtcggcgag catctcgacc agctggcggt actttaccgg ctcggtgtac
     2161 ttgaacgaga gatcctcgag ctcccacttg atctgccaca tccccagccg gtgggccagc
     2221 ggggcgtaga tctccatgct ctggcgggcg atgcgctgct gcttctcggc cggcaggaag
     2281 tgcagggtcc gcatgttgtg cagccggtcg gcgagcttga tgatgaccac ccgcacgtcc
     2341 tcggccatgg ccatgaacat cttccggatg ttctccgcct gctgctcctc catcgagcgc
     2401 gcggacccga acttggagag cttggtgacg ccgtccacca gcttgccgac ctcgcgcccg
     2461 aagcgcttct cgatgtcggc cagggtgagc tcggtgtcct ccgggacgtc gtgcaggagg
     2521 gcggcggcca gcgtctcgac gtccatctgc aacgtggcca ggtagtacgc cacctcgacc
     2581 ggatggctga tgtacggctc gcccgatgcc cgcagctgcc cctcgtgggc catggcggcc
     2641 acctcgtggg cacgctccag caggccgacg ttgatgctcg gccggtgacg acgcgcctcc
     2701 tcgacgacgt cttcgatcgt gtgggtcggc tcagccgctg cctgcccgcg cgaaatgcgc
     2761 tgtaccacgg cggccggagc cgcccgcagg cgggcgaggt ccatggtcgt aagagcgtat
     2821 cacctcggtc atggactcag tagacgtccg ccgatcgcat caccagctcg tggtggtaat
     2881 ggcgacgaat ctcaccgatg gccatcgttc gtggcagaag tcaccgactg ctgagccgtt
     2941 ttcgcgacga atcgggccgg ttgactgcga cgtctggcgc ttcttcgtgg ttagcaccaa
     3001 ctggtgtgat tcggccgtga cggcgttcag gccacgacca cgaaccacgc ctatcggcgc
     3061 agcacgcgtt cgcgggcggc gcggaggcgg taccagaacg gatcggtcac caggtcgaac
     3121 aggccggggt ggcgcagcgg cgtgccgccg aaggtgcgct tgaagtcact gaagcccttc
     3181 caggccgggt cggcctccgg atcatccggc tccacgacgc cacacatgtc gacctggcgg
     3241 cagccgtcga cagccagcgt cttgagcatg ccggccatca ccgcgtgcga gccgtagttg
     3301 tgcttgtagc gctcgtcgcg cagcagggcg ccgtacaggt agtacgcccg atccccgatg
     3361 cgcgggaagg ccatggtggc gatgggcgtg ccctccaccc ggccgatgcc caggtaccac
     3421 ccgccggacg cggcgagctc ggtggccagg cgcgtcagga actggggcga tcggacgcgg
     3481 aactccgcgc gctcagcggc gcgcgacagc aggtcgtgga ggatggcgac ctcgtcctca
     3541 tcggcctggc gaccgatgct gacctcggtc ccctcccgct cagcgcgccg catcaggcgc
     3601 cgcgcgtcgg cgtgccacga ggcctcgagc tgctcgccac cgtcgaggag gtccacgatg
     3661 cgggtggtgg gcgcctgcag gtcgggtgcg gggcgcaggt cgtgctcatc ggccaggtgg
     3721 gtgaagtcga actcctgccc aagctccgcg cgcgggtcca tcttgatgac gagggccctt
     3781 tccttgcggg ccagcgtccg caggccgtgg atcagccagg cgaagacccg gtcggcatcc
     3841 ggcgcggagc gatcccagac cgggccgtgc ggcacgtatg cgacctgacg cccgaacccg
     3901 gccggccgca acagcgcctg ggccacgccg cggacccggc cggcgtcctc caccagcaga
     3961 cgatacgctg gctcgccggc cagggcggtg cactcgcccc acgcccaggc ctggaggaga
     4021 tcggcctctg gtcgggacgc gaggaattcg tcccaggcgg gacggtcggt gtcgtcggcg
     4081 cggcgcacga tgggtggcat cggttcccag agcgtagcgc ggatcggcta gccttcgcgg
     4141 ccatgtcgtc ggagatcgac ctgccctggc agcgccccga gtggcgcgac gaggcgctgg
     4201 cctggattcg cgagcaggtg gctcggcttg gctggcgggt gacgggctcc atcgagcagc
     4261 cgcacgtgat ctggtggtcg agcgtcttcc gcgtccccac ctcggacggc gtcgtgtggt
     4321 tcaaggccgt ccagccggac ggtgcgttcg aggcgcggct cactcccctc ctggccgcag
     4381 actggccgca gcacacggct cacatcctgg cgaccgatgc ggagcgcggc tggacgctgg
     4441 cccgggacgc cggcgcccgc atgcgcgagc tggacgacgg ccgggcggtt gtggagcact
     4501 gggagcggct gctgccgcgc tatgccgaag tgcagctcgg gatggcggac cgccgggatg
     4561 agctgctgcg gctgggcgtg ccggaccgcg ggctgaaatc cctgccgcac gagctgcggg
     4621 cggcgctcga cgagccgcgg gtgctgctca tcggccagga ggacggcctg gacgaggcgg
     4681 accgcgacgc gctcgtggcc gggctggagg agttcagcga gctgtgcgcg actttggccg
     4741 agatgggcat ccccgaatcg atccagcacg acgacctgca cgacggcaac gccttcctcc
     4801 gggacggcga gtatgtcttc ttcgactggg gcgacgcctg cgtctcgcac ccgttccaca
     4861 cgctggtcgt cacgctgagg gcgctggcct accgccagcg ctgggagccc ggcgggccgg
     4921 aggtgatgcg gctgctgtcg gcgtacctgg agccctggac caggttggtg ccgagcgagc
     4981 aggtcctggc cgcggccgac atggcgcgtc gaacggggac catccagcgt gccatggcct
     5041 ggcgagacta cacggcggtc atgccgccgg cggtggccgc cgagaacgtg gactcggtgc
     5101 cctacgggct gcggatgtac ctgctcaacg caccctgggg cagctgggac gacgggacgt
     5161 tctagcggga accagccgga ccgtgccggg cttagtcgtc gctgccggcc agtcggcggc
     5221 ccatcaggcg cagccgcgtg cgttgagcga tgccgtaggc gggcagcagc aggcgcatcg
     5281 cggcatcctc ggccgggcgc agcgggaggt cgcgcgcgcc gacgtactcc acctcgcgcc
     5341 cgccgaagcc ctccttgaac tgggcgatgc cgccggtggc caggccccac atgtcgtaca
     5401 ccgcgaagcc gtccgccttg aagccgagga tggcctgcca cttgagcagg tagttggcgc
     5461 gggagtcggc gccttcatcg gtcattccgc cgaaggtctc ggcggcacgg tcgccgcagg
     5521 tgaagtggaa gagggtggcc acccgccggc cgtcgtggac ggcgaaggcc agccgggcgt
     5581 ggccgcgcgg agcgaacagg ccccagacac gctcgtagta gcgcttggcg cgagccacga
     5641 a
//
LOCUS       DATACA010000326         9238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_203087, whole genome shotgun sequence.
ACCESSION   DATACA010000326 DATACA010000000
VERSION     DATACA010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203087"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VHK28_06660"
     CDS             <1..465
                     /locus_tag="VHK28_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2141944.1"
                     /translation="SRTPPMPHSSSIGPDQSDSLADAWAYAKDYYKSPEVIADFELLA
                     RYRPEVFGGYITLRRAAFNRGPNAALTPREKELIILAIEIGRTKVNPPPVGHARRAIE
                     AGATPADVAEVASLCIMIAGMLSYQESGRHALRAAEERYAQMSSESQPDPGA"
     gene            complement(480..1232)
                     /locus_tag="VHK28_06665"
     CDS             complement(480..1232)
                     /locus_tag="VHK28_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX2141945.1"
                     /translation="MSQPDDTSRPSRPWDGLYGPDQLAVWEAGGYGSRAGLGQRPAVL
                     VIDPVRAFTGYPGEDHLESVKRFRQSCGPYAWEAIPHIQRLLAAGRARGFPVIYTRAS
                     ILPPELRGARRRKNSRVGKESTETAIEGHEYPPEIAPQPGELVIDKTKPSVFFGTPLL
                     SHLQMYGVDHLLVAGTTTSGCVRASVYDAFSLNFSITIPYECVFDRFITSHRVNLFDM
                     NSKFADVISMDELMGQLEEAFPLPGKVAVPAG"
     gene            complement(1290..2243)
                     /locus_tag="VHK28_06670"
     CDS             complement(1290..2243)
                     /locus_tag="VHK28_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2141946.1"
                     /translation="MPEPEMTDIRLGNSGSPTANTMHTILADCENLDEKYGLNIEWLS
                     FDGGSAAAQALLAEQVEVADNSGGPAIASLATDSPMLITYVTRDNLTDNMYAQPDIED
                     AEDLRGGSVAISSFGSQSHAGALLALDVLGLTPDDVTITQVGNDAARLAALQAGSVTA
                     SMNDATQEDELEGLGLNILVRLADEVEAGSGGVPRTSLTFLRPFVEENPNTVLRLVAM
                     YTEANHAWRQDVERSAECLAEAAEIPLDEATAEVEAVLTEPWRPFDGRCDPVVMEFAR
                     QTLVPENPDMADVDPAEACTNEFVEQLADMGFQAELGIEGY"
     gene            complement(2529..3911)
                     /locus_tag="VHK28_06675"
     CDS             complement(2529..3911)
                     /locus_tag="VHK28_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX2141947.1"
                     /translation="MRYASITELSNALRSGQISAVELAEETVARLSEVAPRYNALATA
                     LPDRAREEAAAADRRLANGDASVLCGIPYGAKDLFAARGGPTTWGAPAFADQVFETDA
                     AVIQRLATEGAVLAGKLAMSEFAGGGRPAKPGASLHGQGRNPWNPERYSGGSSSGSGI
                     CVATGLVPFALGTETGGSVLGPAAFSGVTGLRPTVGIVPRGGVMTLSWTLDKVGPLAR
                     SAEDCAIVMDAIATRRPPQGFLAATRQPEARSIRVAFSRAEMDEAAPSIRSALQRGME
                     ELRGLYPNFVDVELERDPGFITSLEDIVRIEGAYGLREHLRREDFVMSDARQLATLRS
                     GLEVSAVDYLEAVRGRVARAQAAFKTVFRKADVILSASRPTIAPRLDEERPPRDATKL
                     SDLLRAAGNLANVPGISFPCGLSDEGMPVGFQLVGPRGSDATLLAMAAAFQRATGHHQ
                     QRPPEPSLPV"
     gene            complement(3919..4755)
                     /locus_tag="VHK28_06680"
     CDS             complement(3919..4755)
                     /locus_tag="VHK28_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141948.1"
                     /translation="MAVATEATTHTPRQQPPVSRLAAWYADHQRLVLGVAGILMILVP
                     WELVVRLGLVKEILVGSPSAVAEAFVREFERGDIWRHIWASLRVWLVGFGLASVAGIF
                     LGLAAGWSRRAGLITVPWMNVAYAAPDLAFVPIFILWFGLGFTFKVFVVFIGVIIYVT
                     LNTIAGVHATEERYLAVARTYGAGRIQMFRSIILPGAVPYIMTGLRIASGRAVVGVIG
                     AEFVSANEGLGFLITISGTTLQTARVFVGIIIIAGFGVLLNEILGRIEGRFDAWRRDI
                     HG"
     gene            complement(4761..5561)
                     /locus_tag="VHK28_06685"
     CDS             complement(4761..5561)
                     /locus_tag="VHK28_06685"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141949.1"
                     /translation="MPELEAQNIQMEYIRRDGSVVRAIDELTLAIEPGEFVSIVGPSG
                     CGKTTFLKIVNGLLEPSAGTLMVRGRPIEQRSGDRAMVFQEAALFPWYTVARNIGYGL
                     ECQKVPKRQIKERVEPYIKMVGLSGFDKHYPYELSGGMQQRANLARALVVNPAVLLMD
                     EPFAALDAQTREMMQSELLDIWSQTNKTVLFITHQISEAIYLSDRVIVMSARPGRIIA
                     DMKIEIPRPRSLAVKRTEKFLKYEDEVWALIESQARATMASDQLTETG"
     gene            complement(5480..6400)
                     /locus_tag="VHK28_06690"
     CDS             complement(5480..6400)
                     /locus_tag="VHK28_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2141950.1"
                     /translation="MTAVPVERRKIPFSKRFAVIEPTVLPLATFAGLIIIWETAARLE
                     LINPFFFSSPSGIVAAGAENILLPEFWNDVWVSSQEFLVGYIAAIAVAVPFGLLTGWF
                     RPLHYLFEPWLAAFNATPRLALLPLVVLWVGLGFWSKAVIVFLGVFFPVAINTFQGVR
                     TVDSNLYDVARSFGASGYRRIMSVVLPSVLPFALVGMKLGIGRAVSGVVVAEFFTSQD
                     GLGNFIFRAGASSIPASCSSARSSSPAWRWGRSLPSAPSSAESRPGNRGSDPHDDSEQ
                     RRGACLSSRPRTSRWSTSVGTARSSGRSTS"
     gene            complement(6397..7491)
                     /locus_tag="VHK28_06695"
     CDS             complement(6397..7491)
                     /locus_tag="VHK28_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141951.1"
                     /translation="MTTTDNPTDVRWLIDAYQVWVETEGVQVVEDVAVDLLAVPTGPW
                     PRMGVDGSFVHLHSRGDLCSLYVLDIPPGGATDTVHHMYEAVYLVLDGQGSTVVEGPE
                     GDQRSFEWGTGSLFGLPLNAPYRLYNSSGRNRARIAVVANLPMVMKQFRDAAFVFGTD
                     RQFPERWGEERFYRGEGIFIPTFEMRHMWETNLVPDVLTFDRMIDSPNRGAGSSNIQF
                     VLGESTMRAHISAVGVGDYKKAHIHGEGVHIIQLGDAGYSLYWLEGQEPRRVDWKFGM
                     LHAPADQEWHQHFNVGDSPGRYLPVSYGNFRYPFTKANVANVLHRYEVKSKIQIEYED
                     EDPRIRQMFNEERAKWRANHSGAGRGTAAS"
     gene            complement(7574..8098)
                     /locus_tag="VHK28_06700"
     CDS             complement(7574..8098)
                     /locus_tag="VHK28_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX2141952.1"
                     /translation="MDPLWLFLVFFRASVFSVGGQSGLPLLRDDLVRTGVLTDVQLIE
                     ALTIGRLSTGPGGLYMVAIGFVAGGWLGAAMALVALALPPLLVLPASAYLRPRLAHRR
                     VNGLIRGLALTTTGLVVAVSVELLWAATPNGNPAIWQFALVVLGVAVSIEGKRHPVIV
                     IAAGAAVGLLLAAQ"
     gene            complement(8105..8710)
                     /locus_tag="VHK28_06705"
     CDS             complement(8105..8710)
                     /locus_tag="VHK28_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX2141953.1"
                     /translation="MTASEPTPEADQPSGRDQLHDWVVIGTQSVGGGASTLYLMRAIL
                     VNRRKWLTLRDFLQEWTLSRLSLGNHLTALAALIGHRIGGRRGVVLAVTGLLIPSGII
                     TAILTGGFGLIRDQPAIQSALAGIAPVTIGMMLGISYVLVRTILSPRWRPLMVDLVVF
                     TAAAVAGFVVAGSTIAIIVTGAVIGTLFLGRDELPPETLEP"
     gene            complement(8707..9111)
                     /locus_tag="VHK28_06710"
     CDS             complement(8707..9111)
                     /locus_tag="VHK28_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX2141954.1"
                     /translation="MSRSFGAQAEELGIPTICAEMGESRRVSWERVPLGVQGIRNVMI
                     DMGMLDEEPVVGDSPRRFSTITLVHAERGGGLRLDVDLGDEVSAGQQIGEVVDVFGER
                     VERLEASTDGFILRAMRYGSIATGAEVVWIAS"
BASE COUNT         1582 a         3148 c         3085 g         1423 t
ORIGIN      
        1 agcaggacgc cgccgatgcc ccattccagc tccattggcc cagaccagtc ggattccctc
       61 gcggatgcgt gggcatacgc caaggactac tacaaaagcc cggaggtcat cgccgacttc
      121 gagctcctgg cgcggtatcg cccagaggta ttcggcggct acatcacgct ccggcgggcg
      181 gccttcaacc gcggcccgaa cgccgcgctc actcctcgtg agaaggagct gatcatcctg
      241 gccatcgaga tcggcagaac caaggtgaac ccgccgcctg tgggccacgc caggcgcgcg
      301 atcgaggcag gagccacgcc ggccgatgtc gcggaggtcg cctcgctgtg catcatgatc
      361 gccggcatgc tctcctacca ggagtcgggc cggcatgcgt tgcgcgccgc cgaggagcgc
      421 tacgcccaga tgtcgagcga gtcgcagccc gatcccggcg cctgaggtgc cgaatgcgcc
      481 tagccggcgg ggacagccac cttgcccggc agtgggaagg cttcctccaa ctggcccatc
      541 agctcgtcca tggagatgac atcggcgaac ttgctgttca tgtcgaacag gttgacgcga
      601 tggctggtga tgaagcgatc gaagacgcac tcgtacggga tggtgatgct gaagttgagg
      661 ctgaaggcgt cgtagaccga tgcgcggacg cagccgctgg tcgtcgtgcc cgccaccagc
      721 agatgatcga cgccgtacat ctggaggtgg ctgagcagcg gcgtcccgaa aaagacgctg
      781 ggcttggtct tgtcgatcac cagctcaccg ggctgcggtg cgatctcggg cggatactcg
      841 tggccttcga tcgccgtctc agttgactcc ttgcccactc gactgttctt gcgccggcgg
      901 gctccgcgca gctccggagg caggatgctg gcacgggtgt agatgaccgg gaagccacgg
      961 gcacggccgg cggccagcag ccgctggatg tgcgggatgg cctcccaggc gtacgggccg
     1021 caggattgcc gaaagcgctt cacagattcc aggtggtcct cgcccggata gccggtgaag
     1081 gcgcgcaccg gatcaatgac cagcacagcg ggccgctggc cgagcccggc ccgcgagccg
     1141 tagccacccg cctcccagac agcaagctga tccgggccgt acaggccgtc ccacggcctc
     1201 gatggtcggg aagtgtcgtc aggctggctc atgattggtt acggcgagca gccgggcagc
     1261 ggtcaaacca cccggccgct cgcgttgcct cagtaccctt cgatgcccag ctcggcctgg
     1321 aagcccatgt cggccagctg ctccacgaac tcgttcgtgc aggcctcagc aggatcgacg
     1381 tccgccatgt ccgggttctc gggaaccagc gtctggcggg cgaactccat cacgaccggg
     1441 tcacaccgac cgtcgaacgg gcgccacggc tcggtgagca cagcctcgac ctcggccgtc
     1501 gcctcatcca gcgggatctc ggcggcctcg gccaggcatt cggctgagcg ctccacgtcc
     1561 tggcgccacg cgtggttggc ctcggtatac atcgccacca ggcgcaggac cgtgttcggg
     1621 ttctcctcca cgaacggtcg caggaaggtc aggctggtac ggggcacgcc gccggagccg
     1681 gcctcgacct catcggcaag gcggacgagg atgttgaggc caaggccctc cagctcgtcc
     1741 tcctgtgtcg cgtcgttcat ggacgcggtc acgctgccgg cctggagcgc ggcaagtcgt
     1801 gccgcgtcat tgccgacctg ggtgatggtc acgtcgtccg gagtcaggcc caggacgtcc
     1861 agcgccagca gcgcgccggc gtgggactgc gatccgaagc tcgagatggc gaccgaccca
     1921 ccgcgcaggt cctccgcgtc ctcgatgtcc ggctgcgcgt acatgttgtc ggtcagattg
     1981 tcgcgggtga cgtaggtgat cagcattggg ctgtcggtgg cgagcgaggc gatggccggg
     2041 ccgccggagt tgtccgcgac ctcgacctgc tcggccagga gcgcctgcgc ggccgccgac
     2101 ccgccgtcga agctcagcca ttcgatattc aggccgtact tctcatcaag gttctcgcag
     2161 tccgcgagga tggtgtgcat cgtgttcgcc gtgggcgagc cggagttgcc gaggcggatg
     2221 tcggtcatct cgggctcggg catggcatcg atcatccccg ccaggtcggt ggctgcggca
     2281 gaaggtgcgg acgatccgtc ggtcgagggc ggttcactcg gctcgccagc cgtcggagat
     2341 gcggcagtcg tgcaggccgc aaggaccaac atgaacacga acgccggcgc gaggatgcgt
     2401 cgaacgggga atgggacgcc catgagttac ctccatcgtc tgcccaaggc gggacctggc
     2461 tcaggcgcgc cagccaaggg tatacagtag catacggctg catccgtcaa gccgttcatg
     2521 ccaagaggct aaacgggcaa cgacggctct ggtggccgct gctggtggtg accggtggcg
     2581 cgctggaatg cagccgccat ggccagcagg gtcgcatcag atccccgcgg acccacgagc
     2641 tggaagccca ccggcattcc ctcgtccgag agcccgcacg ggaaggagat tcccggcacg
     2701 ttggccaggt tgccggcggc ccgcagcagg tctgacagct tggtcgcatc acgtggcggt
     2761 cgctcctcgt ccagccgagg cgcgatggtg ggtcgcgagg cggagagaat gacatcagcc
     2821 ttgcggaaga cggtcttgaa cgcggcctgc gcccgggcca ccctcccgcg gaccgcctcc
     2881 aggtagtcca ccgcggacac ctccagtccc gagcgcagcg tggccagctg gcgcgcgtcg
     2941 ctcatcacga agtcctcgcg ccgcaggtgc tcccgcaggc cataggcacc ttcgatgcgc
     3001 acgatgtcct ccagggaggt gatgaagccc ggatcgcgct ccagctccac gtcgacgaag
     3061 ttcggataca gaccccgcag ctcctccatc ccccgctgca gcgcggaccg gatggatggc
     3121 gccgcctcgt ccatctcggc ccgagagaag gcgacgcgga tgctccgcgc ctccggttgg
     3181 cgagtagccg caaggaatcc ctgcggtggg cggcgtgtcg cgatggcgtc catcacgatg
     3241 gcgcagtcct cggcgctgcg cgccagcggg ccgaccttgt cgagggtcca cgacagggtc
     3301 atcaccccgc cgcgcggcac gataccgacg gtcggtcgca ggcccgtcac tcccgagaac
     3361 gcggctggcc ccaggaccga accgccggtc tcggtgccga gcgcgaacgg caccagcccc
     3421 gtggccacgc agatgcccga cccactcgat gacccgccgg aatagcgctc ggggttccac
     3481 gggttgcggc cctggccgtg cagcgatgcg cccggtttgg caggacgtcc acctccggcg
     3541 aactcggaca tggccagctt gccggccagc accgcccctt cggttgcgag ccgttgaatg
     3601 acggccgcat cggtttcgaa gacctggtcg gcgaatgccg gggctcccca tgtggtgggt
     3661 ccaccccggg cggcaaacag gtccttcgcg ccgtatggaa tgccacacag gaccgatgcg
     3721 tccccgttcg ccagccgtcg gtcggcggcc gccgcctcct cgcgtgcgcg gtccggcagg
     3781 gcggtggcaa gggcgttgta gcgcggtgcc acctcggaca acctggccac cgtctcttcg
     3841 gccagctcga ccgctgaaat ttgcccgctg cgcagcgcgt tgctcagctc ggtgatgctg
     3901 gcgtagcgca cgctcaggtc acccgtggat atcgcgccgc caggcgtcga accggccctc
     3961 gatgcggccg agtatctcgt tcagcagaac cccgaagccg gcgatgatga tgatgcccac
     4021 gaacacgcgc gcggtctgca gggtggtgcc cgagatggtg atcaggaagc ccagcccttc
     4081 gttggccgat acgaactcgg ccccgatgac gccgaccacc gcccggccgg aggcgatcct
     4141 gagcccggtc atgatgtagg gcaccgcgcc gggcaggatg atcgagcgaa acatctggat
     4201 gcgacccgcc ccgtaggtgc gcgccacggc caggtagcgc tcctcggtgg cgtgcacacc
     4261 ggcgatcgtg ttgagcgtga cgtagatgat cacgccgatg aacacgacga acaccttgaa
     4321 cgtgaagccg agtccgaacc acaggatgaa gatcgggacg aaggccaggt ccggtgccgc
     4381 gtacgcgaca ttcatccacg ggacggtgat caagccggcg cgccgggacc agcccgccgc
     4441 caggccgagg aagatgccgg ccaccgatgc cagcccgaag ccgaccagcc agacgcgcag
     4501 tgaggcccag atgtgtcgcc agatgtcgcc gcgctcgaac tccctgacga aagcctcagc
     4561 caccgccgaa ggggagccga ccaggatctc cttgaccagc cccaggcgca cgaccagctc
     4621 ccaggggacc aggatcatca ggatgccggc cacgcccagc accagccgct ggtgatccgc
     4681 gtaccacgcc gccagccggg agaccggcgg ctgctgccgc ggcgtgtgcg tggtggcttc
     4741 ggtggcgaca gccatgctca ctacccggtc tcggtcagct ggtcagaggc catggtcgcc
     4801 cgcgcctggg actcaatgag cgcccacacc tcgtcctcgt acttgaggaa cttctcggtg
     4861 cgcttgacgg ccaaggatcg cggcctgggg atctcgatct tcatgtcggc gatgatccgg
     4921 cccggtcggg cgctcatgac gatgacccgg tcggacaggt agatcgcctc gctgatctgg
     4981 tgcgtgatga agagcaccgt cttgttggtc tgcgaccaga tgtcgagcag ctcggactgc
     5041 atcatctccc gggtctgagc gtcgagggcg gcgaacggct cgtccatgag gagcacggcc
     5101 gggttgacca ccagcgcccg ggccaggttc gcgcgctgct gcatgccgcc cgacagctcg
     5161 tacgggtagt gcttgtcgaa cccgctgagc cccaccatct tgatgtacgg ctccacgcgc
     5221 tccttgatct ggcgcttggg caccttctgg cactcgagcc catacccgat gttgcgggcg
     5281 acggtgtacc acgggaagag cgcggcctcc tggaagacca tggcccggtc cccactgcgc
     5341 tgctcgatgg gccggccgcg gaccatcagc gttcccgcgc tgggctccag cagcccgttg
     5401 acgatcttga ggaacgtcgt cttgccgcac ccgctgggcc caacgatgct cacgaactcg
     5461 ccaggctcga tggcgagcgt cagctcgtcg atcgcccgga cgaccgagcc gtcccgacgg
     5521 atgtactcca tctggatgtt ctgggcctcg agctcaggca cgcgcccctc ctctgttcac
     5581 tgtcgtcatg cggatccgat ccgcggttgc caggccttga ctcggcgctc gacggcgctg
     5641 acggcagcga acgcccccag cgccaagccg gtgatgaaga acgcgccgaa gagcagcttg
     5701 ccggtatcga gctggcgccg gcgcggaaga tgaagttgcc aaggccgtcc tgcgaggtga
     5761 agaactcggc gacgaccacg ccgctgacgg cgcgcccgat gccgagcttc atgccgacca
     5821 gggcgaaggg cagcacgctc ggcagcacca ccgacatgat gcggcggtag ccgctggctc
     5881 cgaagctacg ggccacgtcg tacaggttgc tgtccacggt gcgcacgccc tggaaggtgt
     5941 tgatggccac cgggaagaag acgcccagga agacgatgac cgccttcgac cagaagccca
     6001 gcccgaccca cagcaccacc agcggcagga gcgccaggcg gggcgtcgcg ttgaaggcgg
     6061 ccagccatgg ctcgaacagg taatgcagtg ggcggaacca tccggtgagc aggccgaacg
     6121 gcaccgccac ggcaatggcc gcgatgtagc cgaccaggaa ctcctgcgag ctgacccaca
     6181 cgtcgttcca gaactcgggc agcaagatgt tctcggcacc tgcggccacg atgccgctgg
     6241 gcgagctgaa gaagaacggg ttgatgagct cgagccgcgc cgcggtctcc cagatgatga
     6301 tcagcccagc gaaggtggcc agcggcagga ccgtgggctc gatgaccgca aagcgcttgc
     6361 tgaaggggat cttccgccgt tcgacgggta cggctgtcat gaagctgccg tccctcggcc
     6421 ggcgccgctg tggttggcac gccacttcgc gcgctcttcg ttgaacatct gacggatcct
     6481 ggggtcctcg tcctcgtact cgatctgaat cttgctcttc acctcgtagc ggtggagcac
     6541 gttcgccacg ttggccttgg tgaacggata gcggaagtta ccgtacgaga ccggcaggta
     6601 gcgtccggga ctgtcgccga cgttgaagtg ctgatgccac tcctggtcgg ccggcgcgtg
     6661 cagcatgccg aacttccagt ccacgcgacg cggctcctgg ccctcgagcc agtaaagcga
     6721 gtagccggca tcccccagct ggatgatgtg cacgccttcc ccgtggatat gcgccttctt
     6781 gtagtccccg acacccaccg cggagatgtg agcacgcatg gtcgactctc cgaggacgaa
     6841 ctggatgttg gagctgccgg cgccgcggtt cggcgagtcg atcatgcggt cgaaggtgag
     6901 gacgtctggg accaggttgg tctcccacat gtggcgcatc tcgaaggtcg ggatgaagat
     6961 gccctctccc cggtaaaaac gttcctcgcc ccaccgctcc gggaactgtc gatccgtccc
     7021 gaagacgaag gccgcgtctc ggaactgctt catgaccatg ggaaggttgg cgaccacggc
     7081 gatccgtgct cggttgcggc cgctcgagtt gtacaaacgg tacggggcgt tgaggggaag
     7141 gccgaagagg ctgccggtac cccactcgaa gctccgctgg tcaccctccg gtccctcgac
     7201 cacggtgctg ccctgcccgt cgagcaccag gtagaccgcc tcgtacatgt gatggacggt
     7261 gtcggtggcg ccgcccggtg gaatgtccag cacgtagagc gagcagaggt cgccgcgcga
     7321 gtgaaggtgg acaaagctcc cgtccacccc catgcgaggc catgggccgg tggggacggc
     7381 caggaggtca accgcgacgt cctccacgac ctggacgccc tcagtctcca cccagacctg
     7441 gtaggcgtcg atcagccagc ggacgtcggt cgggttgtcg gtcgttgtca ccgtgcctgc
     7501 cccttctcgc ttggcatcgg gagatgatag cggccgtctg tgtgccactg tatacgaaac
     7561 gtgcaccgca ctgctactgg gcggccagga gcagtccgac cgccgctccc gcggcgatga
     7621 cgatgacggg atgacgcttg ccctcgatgc tgaccgccac gccgaggaca acgagcgcaa
     7681 actgccagat cgccgggttg ccgttcgggg tggctgccca cagcagctcg acgctgacgg
     7741 caaccaccag gccggtggtc gtcagcgcca gaccacgaat cagcccgttg acccggcggt
     7801 gcgccagccg ggggcgcagg tatgcggacg ccggcagcac cagcagcgga ggcagcgcca
     7861 gcgcaaccag cgccatcgcc gcacccagcc aaccgccggc gacaaagccg atggcgacca
     7921 tgtacagccc acctgggccg gtcgacaggc gcccgatggt cagcgcctcg atcagctgca
     7981 catcggtcag caccccggtt cgcaccaggt cgtcgcgaag cagcggaagg ccgctctgcc
     8041 caccgacgga gaacaccgag gccctgaaga agacgaggaa cagccagagc ggatccatcg
     8101 gtgcctatgg ctcgagcgtc tcgggtggca gctcgtcgcg gccaaggaac agcgtgccga
     8161 tcacggcccc ggtgacgatg atggcgatgg tcgaaccggc gaccacgaag ccggcgaccg
     8221 ccgcggcggt gaagacgacc aggtccacca tcagcggccg ccagcgaggg ctgaggatcg
     8281 tccgcaccag gacgtaggag atgccgagca tcatcccgat ggtcaccggc gcgatcccgg
     8341 ccagcgccga ctggatggcc ggctggtcac ggatcaagcc gaagccaccg gtcaggatgg
     8401 cggtgatgat ccccgacggg atgagcaggc ccgtgaccgc cagcaccacg cccctgcgac
     8461 cgccgatgcg atgcccgatc aaggcggcca gcgcggtcag gtggttgccc aggctcagcc
     8521 tggagagagt ccattcctgc aggaagtcac gcagcgtcag ccacttgcgg cgattcacca
     8581 ggatcgcgcg catcaggtac agcgtgcttg ctccgccacc caccgattgg gtgccgatca
     8641 cgacccagtc gtggagctga tcgcggccgc tcggctggtc ggcctcaggc gtcggctcgg
     8701 acgccgtcac gacgcgatcc acaccacctc ggctccggtg gcgatcgagc cgtagcgcat
     8761 ggctcgcagg atgaagccgt cggtcgaggc ctccaggcgc tccacccgct cgccgaagac
     8821 gtcaaccacc tcgccgatct gctggccggc gctcacctcg tctccgaggt cgacatcgag
     8881 gcgcagccct ccaccccgct cggcgtggac gagcgtgata gtcgaaaagc ggcgaggcga
     8941 gtcccccacg accggctcct cgtcgagcat gcccatgtcg atcatcacgt tgcgaatgcc
     9001 ctgcacgccg agggggacgc gctcccagct gacccgccgc gactccccca tctcggcgca
     9061 gatggtcgga ataccgagct cctcggcctg cgcaccgaag ctgcggctca cgtcgctggt
     9121 gcccagcttc aaaccccgtc gcttgtagta ggcgtagggg gtgccgaagc gaggccccgg
     9181 cgctcccgca cctcaaaggt ccccttttcg tcgtcgtccg ggtcgatgta cacgaagg
//
LOCUS       DATACA010000327         5334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_362750, whole genome shotgun sequence.
ACCESSION   DATACA010000327 DATACA010000000
VERSION     DATACA010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362750"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VHK28_06715"
     CDS             complement(<1..1151)
                     /locus_tag="VHK28_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX2141955.1"
                     /translation="MAGLTELFETNRVIVLSVYGQVFFVMGLAIALQTLRRSALSLAR
                     PLPWLAGFGIVHGFHEWGYLFIPIQSGYLPLAATEGLLVLQLVIKGISFALLLQFGVE
                     LLAAVSRLPILPRLRLLPAAALLGWVGATLAVSAAVGPHTPDAGAWLAEGRIDEALQV
                     VGTPLAVGDVLARWMLALPGAAMAAWGLAASAAQVRPVARTPVVAGLRVAAVAFAAYA
                     FLGGAVGMSAPFAPASVLNGAALAEASGLPIEVLRSLTGLVIAVAIILALDLFEQETD
                     RALAEARRRELLARERERIGRDLHDGIIQSIYAAGIHLEEAGAALDPGSDAPRARIQT
                     VLHELEHISGELRRTIFDLRTASLETLDPEEIVRSVADELRANSLVAVDL"
     gene            1204..1605
                     /locus_tag="VHK28_06720"
     CDS             1204..1605
                     /locus_tag="VHK28_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141956.1"
                     /translation="MRSAPLPVKLWFGWAFALLAASGLLLPQIIAFVDFSERAPFSLL
                     GIFMMAELAAVLFGITVALQRKRIGHRFAIGIAALAVPMLAGLPPALFRMEPAAANGW
                     YAAAIPIGLLISVVLIIGLLRPSARAWFIED"
     gene            1631..2536
                     /locus_tag="VHK28_06725"
     CDS             1631..2536
                     /locus_tag="VHK28_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2141957.1"
                     /translation="MKLLAYARGGPTRLGVQSSDGSIHDLTDALAVADVGELLAAGRT
                     AREVEDLRVRADRFAPEEVSLRAPIARPPKIICVGLNYHDHCREQNIEPPAYPMLFSK
                     FANAVSGPRDTVTRPRATEKLDLECELAVIIGRRTSRISREQALDAIFGYTILNDVTM
                     RDLQREDRQWLRAKGSDGFAPIGPVVVTRDELGDGSGLRLRSSVNGQTWQDSTSDEMV
                     FDVPTIVSFASQTITLEPGDVIATGTPAGVGHYQDPPRYLADGDLMRCEIDGIGALEN
                     AVVDELPRIAEHAAAAGIAGLEQGR"
     gene            2533..3585
                     /gene="tdh"
                     /locus_tag="VHK28_06730"
     CDS             2533..3585
                     /gene="tdh"
                     /locus_tag="VHK28_06730"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX2141958.1"
                     /translation="MTSGVAAATMRALAKTRAEPGAELIERAVPTPGPGEVLLRMEAA
                     SICGTDYHLYRWDPWAREVLTPPVILGHELAGRVAATGPGVSRVREGDLVGVESHIVC
                     WECSQCRAGEMHLCRDLKVIGAHVDGGFAEYVVIPESNAVESNGLEPAVVALQEPMGN
                     AVHAAFVEPLEGRSVAVTGAGPIGLLSVAIARAAGASWVVATDVEPYRLDLARQMGAD
                     LALDAREEGTADRIVEETGGDGVDVVLEMSGSQAALDQALHFITRGGRISLLGIFASP
                     VEVHLSDLVIQKGLRLYGIYGRRMYETWERTQSLLRSGAVDPQPLITHRFDLAEWERG
                     FELIGSRHAGKVVLLP"
     gene            3708..4928
                     /locus_tag="VHK28_06735"
     CDS             3708..4928
                     /locus_tag="VHK28_06735"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX2141959.1"
                     /translation="MTDALARARARNPLAFLDAEIEELRAKGLFRRLRVVESEQKSRC
                     VIDGREVITLSSNNYLGLNTHPRLREAAERAVREYGAGSGAVRTIAGTMSLHEELESR
                     LAAFKNVEAVLTFQSGFTCNTGVIPILAGEGSVIVSDALNHASIIDGIRLTKAERKIF
                     PHGDMDALRAALRDVRDAPGGADRTVLVITDGVFSMDGDIARLPEIAEAAEEAEAIVY
                     VDDAHASGVLGRNGRGSIDHFDLHGRVQVQVGTLSKAVGVLGGYVAGPAALRTVLEHR
                     ARPFLFSTSHPPAVAAACLAAIGVLEDEPELIDRLWENTRFFKDGLARLGFDTGASET
                     PITPVIVGAGALAMKLSDRLFEEGVFAQGIGFPTVPEDRSRVRTIVTAEHSRSELETC
                     LDAFERVGRELAII"
     gene            5055..>5334
                     /locus_tag="VHK28_06740"
     CDS             5055..>5334
                     /locus_tag="VHK28_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141960.1"
                     /translation="MPERQLSALVVKALRDAERRPPAAGSAERPMPLPAGDDAQVPGG
                     IDELAAAAARGNTEAVGQLYDALVGPIFRYVAVRVHRREDAEGLTQLVF"
BASE COUNT          796 a         1919 c         1879 g          740 t
ORIGIN      
        1 aggtcgaccg ccaccagact gttggcgcgc agctcgtccg caacgctgcg cacgatctcc
       61 tccggatcga gcgtctcgag gctggcggtg cgcaggtcga agatcgtgcg ccgcagctcg
      121 ccactgatgt gctccagctc gtgcaggacg gtctggatcc tggcgcgcgg cgcgtccgag
      181 cccggatcga gcgcggcgcc cgcctcctcc aggtggatgc cggcggcgta gatggattgg
      241 atgatgccgt cgtgcaggtc gcgcccgatg cgctcgcgct cgcgcgccag gagctcccgg
      301 cggcgcgcct cggccagggc acgatcggtc tcctgctcga acaggtccag ggccaggatg
      361 atggccaccg cgatgaccag cccggtgagg gaacgcagga cctcgatcgg caggccgctg
      421 gcctccgcca gggccgcgcc attgaggacc gatgccggcg cgaacggggc cgacatgccc
      481 accgctccgc cgaggaacgc ataggcggcg aacgccacgg ccgccacccg cagtccggcc
      541 accaccggcg tgcgggccac gggacgcacc tgtgcggcgc tggccgccag gccccaggcg
      601 gccatcgcgg cgccgggcag cgccagcatc cagcgcgcga gcacgtcacc caccgccagc
      661 ggggtgccga ccacctgcag ggcctcgtcg atgcgcccct ccgccagcca ggcgccggca
      721 tcgggagtgt gcggcccgac ggcggcagag acggccaggg tggcgccgac ccagcccagc
      781 agcgccgcag cgggcagcag ccgcagccgc gggaggatcg gaagccggct caccgccgcc
      841 agcagctcga cgccgaactg cagcagcagc gcgaagctga tgcccttgat gaccagctgg
      901 aggaccagca ggccctcggt tgccgccagc ggcaggtagc cggattggat gggaatgaac
      961 aggtagcccc actcatggaa gccgtggacg atgccgaatc ctgcgagcca gggcagcggc
     1021 cgagccagag acagcgcgct gcgccgcagg gtctgcagcg cgatggccag ccccatcacg
     1081 aagaagacct gcccgtagac ggacagcacg atgactcgat tggtctcgaa cagctcggtc
     1141 aggcccgcca tggaccgatg ttaggccgcc ggtgcgcgag cgctgctagc atcggccgga
     1201 cccatgcgca gcgcaccact tcccgtcaag ctctggttcg gctgggcgtt cgcgctgctc
     1261 gccgccagcg gtctgctgct gccgcagatc atcgccttcg tggacttcag cgagcgggcg
     1321 ccgttcagcc tgctcggcat cttcatgatg gccgagctcg cggcagtgct cttcggcatc
     1381 acggtcgccc tgcagcgaaa gcgcatcggc caccgcttcg ccatcggcat cgccgccctg
     1441 gccgtaccca tgctggccgg gctgccgcca gctctgttcc gcatggagcc ggcggcagcc
     1501 aacggctggt acgcggccgc cattccgatc gggctgctga tcagcgtggt gctcatcatc
     1561 ggcctgctgc ggccctcggc ccgggcctgg ttcatcgagg actgagcctg tcgctcctat
     1621 actgccgccc gtgaagcttc tcgcctacgc gcgaggcggc ccgacccggc tcggggtcca
     1681 gtcctccgac ggcagcatcc atgacctgac cgatgcgctc gcggtcgccg acgtcggcga
     1741 gctgctggcc gccggccgca cggcccgcga ggtcgaggac ctgcgcgtcc gggccgatcg
     1801 cttcgcgccc gaggaggtgt cgctccgcgc accgatcgcc cgcccgccga agatcatctg
     1861 tgtcggcctc aactaccacg atcactgccg cgagcagaac atcgagccac cggcctaccc
     1921 gatgctgttt tcgaagttcg ccaacgcggt atcgggtccc cgcgacacgg tgacccggcc
     1981 gcgggccacc gagaagctcg acctcgagtg tgagctggcg gtcatcatcg ggcgccgcac
     2041 gagcaggatc agccgcgagc aggcgctgga cgcgatcttc ggctacacca tcctcaacga
     2101 cgtgaccatg cgtgacctgc agcgagagga ccgccagtgg ctgcgggcga aggggtccga
     2161 cgggttcgcg cccatcggtc cggtggtggt cacccgcgac gagctgggcg acgggtccgg
     2221 actccggctg cgctccagcg tgaacggcca gacgtggcag gactcgacga gcgacgagat
     2281 ggtcttcgac gtaccgacca tcgtctcgtt cgcgtcgcag accatcaccc ttgagcctgg
     2341 cgacgtgatc gcgaccggca cgccggcggg cgtgggccac taccaggatc ctccgcgcta
     2401 cctggccgat ggcgacctca tgcgctgcga gatcgacggc atcggcgctt tggagaacgc
     2461 ggtggtcgac gagctgccgc ggatcgcgga acacgccgcg gcggcgggca tcgccggcct
     2521 ggagcagggt cgatgaccag cggcgtggcc gccgcgacca tgcgcgccct ggccaagacc
     2581 cgtgccgagc ccggcgcgga gctgattgag cgcgcggtcc caactcccgg ccccggcgag
     2641 gtgctgctgc gcatggaggc cgccagcatc tgcggcaccg actaccacct ctaccgctgg
     2701 gacccgtggg cacgcgaggt gctcacgccg ccggtcatcc taggccacga gctggccgga
     2761 agggttgccg ccaccggacc cggcgtgtcc cgcgtccgcg agggtgacct ggtcggcgtc
     2821 gagagccaca tcgtgtgctg ggagtgctcg caatgccgag ccggcgagat gcacctgtgc
     2881 cgcgacctga aggtgatcgg cgcccacgtg gacggcggct tcgccgagta cgtcgtcatc
     2941 ccggagtcga acgcggtgga gtccaacggc ctggagccag cggtggtcgc cctccaggag
     3001 ccgatgggca acgccgtcca cgccgccttc gtggagccgc tggagggccg gtcggtggcc
     3061 gtcaccgggg ccggaccaat cggcctgctg tcggtggcca tcgcgcgggc ggccggggcg
     3121 tcctgggtgg tggcgaccga cgtcgagccc taccgcctgg atctggcgcg ccagatggga
     3181 gcggacctgg ccctcgacgc ccgggaggag ggcaccgccg accgcatcgt ggaggagacc
     3241 ggtggcgacg gcgttgacgt ggtgctcgag atgagcggct cgcaggccgc actggaccag
     3301 gcgctgcact tcatcacccg tggcggccgc atcagcctgc tcggcatctt cgcctcgccg
     3361 gtggaggtcc acctctccga cctggtcatc cagaagggcc tgcgcctgta cgggatctac
     3421 gggcgtcgca tgtacgagac ctgggagcgg acgcagtcgc tgctgcgatc cggggccgtg
     3481 gacccgcagc cgctgatcac ccatcgcttc gacctggcgg aatgggagcg cggcttcgag
     3541 ctgatcggct cccgccacgc cggcaaggtg gtcctgctgc cctaggcctg ccaccgtcgg
     3601 cccggattca cgcccggcgt gaaatcggcg gccccaggga ccacaatggc agcgtaccgc
     3661 acgcctctag tgaattcgga cgattcgagc caagggaggc cgatggaatg accgatgcac
     3721 tcgcgcgggc ccgcgctcgc aacccactcg ccttcctgga cgccgagatc gaggagctca
     3781 gggccaaggg cctcttccgc cggctgcgag tcgtcgagag cgagcagaag agccgatgcg
     3841 tgatcgatgg gcgcgaggtc atcaccctct cgtcgaacaa ctacctgggg ctgaacaccc
     3901 atccccgcct gcgcgaggcg gccgagcggg cagtgcgcga gtacggcgcc gggtccggcg
     3961 cggtgcggac catcgccggc accatgagcc tccacgagga gctcgagtcg cgcctcgccg
     4021 ccttcaagaa cgtggaggcg gtgctcacct tccagtccgg cttcacctgc aacaccgggg
     4081 tcatcccgat cctcgccggc gagggttccg tgatcgtctc cgacgcgctg aaccacgcca
     4141 gcatcatcga cggcatccgg ctcaccaagg ccgagcgcaa gatcttcccc cacggcgaca
     4201 tggatgccct gcgggcggcg ctgcgcgacg tccgcgacgc gccgggcggc gccgatcgca
     4261 ccgtgctggt catcaccgac ggcgtgttca gcatggacgg cgacatcgca cgcctgcccg
     4321 agatcgcgga ggccgcggag gaggcggagg ccatcgtgta cgtggacgat gcgcacgcgt
     4381 cgggcgtgct gggccgcaac ggccggggca gcatcgacca cttcgacctc cacggccggg
     4441 tccaggtcca ggtggggacc ctctccaagg cggtcggcgt gctgggtggc tacgtggccg
     4501 gccccgccgc cctgcgcacc gtgctcgagc accgtgcgcg acccttcctc ttctccacct
     4561 cgcacccacc ggcggtcgcg gcggcctgcc tggccgccat cggcgtgctc gaggacgagc
     4621 cggagctcat cgaccggctg tgggagaaca cccgcttctt caaggacggg ctggcccggc
     4681 tcggcttcga caccggcgcc tccgagacgc cgatcacccc ggtgatcgtg ggggccgggg
     4741 cgctggccat gaagctctcc gaccggctct tcgaggaggg ggtcttcgcc cagggcatcg
     4801 gcttcccgac ggttcccgag gatcggtcgc gggtgcgcac catcgtcacg gccgagcaca
     4861 gccggtctga gcttgagacg tgccttgacg cattcgagcg ggtcggccgg gagctggcca
     4921 tcatctgagc gccgctccaa ccaccgcgag cacgattcac gctcagcggg gctggtgccg
     4981 aagcccgcgt tcgggtaccg tggcggctcc gtacggcgtg cggctgccca cgccagtatc
     5041 aggtgcccag cacgatgccc gagcgacagt tgagtgccct cgtcgtcaag gctctgcgcg
     5101 acgcagaacg ccggccgcct gccgcgggca gcgccgagcg cccgatgccc ctgccagccg
     5161 gcgacgacgc ccaggtgccg ggcggaatcg atgagctggc cgcggccgcc gccaggggaa
     5221 acaccgaggc cgtgggccag ctgtacgacg cgctggtggg gcccatcttc cgctacgtgg
     5281 cggtccgcgt ccaccggcgc gaggacgcgg agggcctgac ccagctggtg ttcg
//
LOCUS       DATACA010000328         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1487195, whole genome shotgun sequence.
ACCESSION   DATACA010000328 DATACA010000000
VERSION     DATACA010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487195"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1077)
                     /locus_tag="VHK28_06745"
     CDS             complement(70..1077)
                     /locus_tag="VHK28_06745"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2141961.1"
                     /translation="MGDIGGRAAVAARQRRGANGELSAWTDGLLVAVAMAYAAVVALL
                     TLVRGIPLTLDVLLVATGFVLVMVARKRLGALSEWIPFVVLFLAYELMRGLADEVGLP
                     VHVGEVAALERSLFGGQLPTAVLQGWLHPASGTDWLAVAGTVVYYFHFVLPVVTALLL
                     WRRVPLLFHPYLISLILLSFAGFATYLLLPVAPPWMAAQLGQIGATTSEPMVHDLKLH
                     AFTQVTGAVGLDGESLYDVAFIALSANPVAAFPSLHAAFAFLSFLVLRRAFGRAGWMG
                     LVYFGMVAFSIVYSADHYVIDVLAGVTYASAAYGLMWLLVRPRMEPAEPSPNVPQAAP
                     G"
     gene            complement(1121..2083)
                     /locus_tag="VHK28_06750"
     CDS             complement(1121..2083)
                     /locus_tag="VHK28_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2141962.1"
                     /translation="MATLTDTDLRQRNERWLVVAVVGYAALVVGIMIAGGLSITPDVL
                     FVAFALAAVLLGRGRVFLRDWLPFIAIFLAYELMRGIADDAGFPVHVEDVIAAERLIS
                     FGQLPTQLLQEWLASGSGVSAAALAATVLYMLHFALPVVTGFFLWLWRRPQYYDFVGA
                     FIVLSFAGFVTFLLMPVAPPWYAANLGALNGPDGEPVVRYLKPDAFAVLADAMGFDGR
                     WLTSYVFYGINPNGVAAFPSLHAGYPFLTFLVLRRAFGPIGYLGLLYALAVWLAIVFT
                     GDHYLIDALAGAAYATAAYFGTRLLSDMLSRRGKVKAAEAQGSG"
     gene            complement(2132..2533)
                     /locus_tag="VHK28_06755"
     CDS             complement(2132..2533)
                     /locus_tag="VHK28_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2141963.1"
                     /translation="MRPRFATAAATLLALGVLAAGCGTTRPVEIQITVHYSKFEPSAV
                     SVPSGVPVTFVLVNADPIDHEWLIGDPDFHQRHRTGTDAQHGARPNEISVPALASEST
                     TLTFEEPGELAFICHLPGHETYGMVGKLTVL"
     gene            complement(2543..>2752)
                     /locus_tag="VHK28_06760"
     CDS             complement(2543..>2752)
                     /locus_tag="VHK28_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141964.1"
                     /translation="DASGMNAPSLIDAGFAPFGPLPAPPPPAAVWVLLLGAALVLPLG
                     GAWMLAMAARGGAGGTAGGRPLAGA"
BASE COUNT          510 a          939 c          971 g          332 t
ORIGIN      
        1 ccggctactt cttcggcagc ctcctgcccg gcatgctggg cgtcccgccg gtgggcgcct
       61 gaggcactct cagccggggg ccgcctgcgg aacgttgggc gacggctcgg caggctccat
      121 ccgcggacgg acgaggagcc acatcagccc gtacgcggcc gacgcgtagg tcacgccggc
      181 cagcacgtcg atgacgtagt gatcggccga gtacacgatg ctgaaggcca ccatcccgaa
      241 gtacaccagc cccatccagc cggccctgcc aaaggcccgc cgcagcacca ggaaggagag
      301 gaacgcaaac gcggcgtgca gcgaggggaa ggcggccacc gggttcgccg acagggcgat
      361 gaaagccacg tcgtacaggc tctcgccatc caggcccaca gcaccggtga cctgcgtgaa
      421 ggcgtgcaac ttcaggtcgt gcaccatcgg ttccgaagtg gttgcaccga tctgccccag
      481 ttgagcagcc atccagggcg gcgccaccgg gagcagcagg taggtggcga agccggcgaa
      541 gctgagcagg atgagggaga ttaggtacgg gtggaacagc agcgggacgc gccgccacag
      601 cagcagcgca gtgacgaccg gcaggacgaa gtggaagtag tagacgacgg tgcctgccac
      661 cgccagccaa tcggtccccg atgccggatg cagccatccc tggagcacgg cggtcggcag
      721 ctgcccgccg aacagagagc gctccagggc ggccacctcg ccgacgtgga ccggcaggcc
      781 gacctcgtcc gccaggccgc gcatcagctc gtaggccagg aacagaacca cgaacgggat
      841 ccactcgctg agggcgccga gccgcttcct ggcgaccatg accagcacga agccggtggc
      901 caccagcagg acgtccaggg tcagcgggat gccgcgcacc agcgtgagca gggcgaccac
      961 agcggcgtac gccatggcca cggcgaccag caacccatcg gtccaggccg atagctcgcc
     1021 gttcgcgcct cggcgctggc gcgcggcgac ggccgcgcgg ccgccaatgt cgcccatcac
     1081 gctcctctgg ggttgctcgg atcgacgcag gatagacgcc tcagccgctt ccctgcgctt
     1141 cggccgcctt caccttgccc cgccgcgaga gcatgtcgct gaggaggcgg gtgccaaagt
     1201 aggcggcagt ggcgtaggct gcgccggcca gcgcgtcgat caggtagtgg tcgccggtga
     1261 acacgatcgc caaccagacg gccagggcat agagcaggcc gaggtagccg atgggcccga
     1321 atgcccgccg cagcaccagg aaggtcagga acgggtagcc ggcgtgcagc gacgggaagg
     1381 cggccacgcc gttcgggttg atgccgtaga agacgtacga ggtcagccat cggccgtcga
     1441 agcccatcgc atcggccagg acggcgaagg catccggctt caggtagcgg accaccggtt
     1501 cgccatccgg cccgttcagt gcgccgaggt tcgccgcgta ccatggcggc gccaccggca
     1561 tgagcaggaa ggtgacgaac ccggcgaagc tcagcacgat gaaggcaccc acgaagtcgt
     1621 agtactgcgg gcggcgccac agccacagga agaagccggt caccaccggc agggcgaaat
     1681 gcagcatgta cagcacggtg gcggccaggg ccgcggcgct caccccggac ccggacgcca
     1741 gccactcctg gaggagctga gtgggcagct ggccgaagga gatgagccgc tctgcggcga
     1801 tcacgtcctc gacgtgcacc gggaacccgg cgtcgtcggc gatgccgcgc atcagctcgt
     1861 aggccaggaa gatggcgatg aatggcagcc agtcgcgcag gaagacgcgt ccccggccca
     1921 gcagcacggc ggccagtgcg aaggccacga acaggacgtc gggcgtgatc gacaggccgc
     1981 cggcgatcat gatgccgacc accagcgccg cgtagccgac cacggcgaca accagccagc
     2041 gttcgtttcg ctggcgcagg tccgtatcgg tcagcgtcgc catgacgaca cggtgggcat
     2101 accggcgggc atagtggcgg agtataggag cctacagaac ggtcagtttt cccaccattc
     2161 cgtacgtttc gtggcccggc aggtggcaga tgaaggccag ctccccgggc tcctcgaagg
     2221 tgagggtcgt cgactccgac gcgagcgccg gcacgctgat ctcgttcggg cgggcgccgt
     2281 gctgggcatc cgtcccggtg cggtgccgct ggtggaagtc cgggtcgccg atcagccatt
     2341 cgtgatcgat cgggtcggcg ttgaccagca cgaaggtcac cggcactcca ctcggcacgc
     2401 tcaccgctga tggctcgaac ttcgagtagt ggaccgtgat ctggatctcg accggccgcg
     2461 tcgtcccgca gccggcggcc aacacaccca gcgcgagcaa ggtggcggcc gccgtcgcga
     2521 agcggggacg catcggttcc gcctaggccc cggccaacgg ccgaccgccg gcggtcccac
     2581 cggcgccgcc ccgggcagcc atggcgagca tccaggcacc ccccagcggc aggaccagcg
     2641 cagccccgag gagcaggacc cataccgcag ccggtggtgg tggtgccggc agcggaccga
     2701 agggcgcgaa gccggcatcg atcagcgacg gggcgttcat gcccgaggcg tc
//
LOCUS       DATACA010000329         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2772459, whole genome shotgun sequence.
ACCESSION   DATACA010000329 DATACA010000000
VERSION     DATACA010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772459"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VHK28_06765"
     CDS             <1..234
                     /locus_tag="VHK28_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX2141965.1"
                     /translation="ATPGPTPTPAPGTVQVPNTIGLSEGEAEAAARAAGLNWRIEWIV
                     DPAQPPGIYDQEPAAGTVVEAGSRLVMYAYRSG"
     gene            327..>1709
                     /locus_tag="VHK28_06770"
     CDS             327..>1709
                     /locus_tag="VHK28_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX2141966.1"
                     /translation="MRFRAESADPASVQADVLAVPIYKEDREMSGDLAQLDAASGGVI
                     SRAIEWGEFNLLEHYTALIDAGSLAAGHLLLVNAVRRGRGAWRARRMASTATRRLQGH
                     GVERMALWLRDGEDEDGYTAAVVGAMQGTYRPFATYGRVRDTPAMLRSVPEVLLVGEG
                     APAQEVIDRAAHIAEGVEFGRTLANRATNDLYPEKMAEVARELTADGCQVEVLGVEEM
                     TALGMGALLGVGQGAAHPPVLIGIKLPGWEKASPDRRLAIVGKGVCFDSGGISIKPSE
                     RMEEMKHDKSGAAAVVAAARTLARIAPDAPVMAVAPMVENMPGGNAQRPGDVVKAMNG
                     KTIEVTNTDAEGRLILADALHWAETQGATHLVDVATLTGAAAIAFGELISAYFAKPRD
                     WGAEVQRAADESGEWFWEMPLATEYRPAYDSAHADMVNSGTRDASLIKSAVFLSEFAT
                     VPWVHVDIAGS"
BASE COUNT          259 a          592 c          613 g          245 t
ORIGIN      
        1 gccacgcccg ggcccacgcc cacgccggcg ccgggcaccg tccaggtacc gaacaccatt
       61 ggcctctccg agggggaggc ggaggccgca gcgcgcgcgg ccgggctcaa ctggcgcatc
      121 gaatggatcg tggaccccgc acagccgccc ggcatttacg accaggaacc agcggctgga
      181 accgttgtag aggccggctc gcgcctcgtg atgtacgcct accgctcggg ttaggccctc
      241 gctcgtccct atacttgccg ctgccggagg ctgtgcccac gccatcggtc catcaccctt
      301 cttgaagcga acctgaggag ttacgaatgc gcttccgtgc cgagtcggct gacccggcat
      361 cggtccaggc cgatgtgctg gctgttccga tctacaaaga ggaccgcgag atgagcggtg
      421 acctcgccca gctggacgcg gcgtccgggg gagtgatcag ccgggccatc gagtggggcg
      481 agttcaacct cctcgagcat tacacggcgc tgatcgacgc cggttcgctt gccgccggcc
      541 atctcctcct ggtcaacgcc gtgcggcggg gacgcggggc ctggcgggct cgccggatgg
      601 cctccaccgc gacccggcgg ctgcaggggc acggcgtcga gcgcatggcg ctctggctgc
      661 gggacggcga ggatgaggac ggctacaccg ccgccgtggt gggcgccatg cagggcacct
      721 accgtccctt cgccacgtac gggcgcgtcc gcgacacccc ggccatgctc cgcagcgtcc
      781 ccgaggtgct gctggtcggc gagggtgctc ctgcccagga ggtcatcgat cgcgcggcgc
      841 acatcgcgga gggggtcgag ttcggacgca cgctcgccaa ccgggcgacc aacgacctct
      901 atccggagaa gatggccgag gttgcgcgcg agttgaccgc ggacggctgc caggttgagg
      961 tgctcggcgt cgaggagatg accgccctgg gcatgggcgc gctgctgggg gtcggccagg
     1021 gggccgcgca cccgccggtg ctgatcggca tcaagctgcc cggctgggag aaggcctcac
     1081 cggatcgccg gctggcgatc gttggcaagg gcgtctgctt cgactcgggg ggcatcagca
     1141 tcaagccctc cgagcgcatg gaggagatga agcacgacaa gtcgggggcc gccgcggtgg
     1201 tggctgccgc gcgcaccctg gcccgcatcg cgccggacgc cccggtcatg gcggtggccc
     1261 ccatggttga gaacatgccg ggcggcaacg cccagcggcc gggcgacgtg gtcaaggcca
     1321 tgaacggcaa gaccatcgag gtcaccaaca ccgatgccga gggccgtctg atcctggccg
     1381 atgccctcca ttgggctgag acgcaggggg ccacccacct cgtggacgtg gccacgctca
     1441 ccggcgccgc ggccatcgcg ttcggagagc tcatctccgc ctacttcgcc aagccgcgag
     1501 actggggcgc ggaggtgcag cgagcggcgg acgagagcgg tgagtggttc tgggagatgc
     1561 ccctggccac cgagtaccgg ccggcctacg actccgccca cgctgacatg gtcaactccg
     1621 gcacgcgcga cgcgtcgctc atcaagagcg ccgtcttcct gtcggagttc gccaccgttc
     1681 cctgggtgca cgtggacatc gccggcagc
//
LOCUS       DATACA010000330         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1969417, whole genome shotgun sequence.
ACCESSION   DATACA010000330 DATACA010000000
VERSION     DATACA010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969417"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VHK28_06775"
     CDS             complement(<1..186)
                     /locus_tag="VHK28_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141967.1"
                     /translation="MVEDEALLALELEQVLSEAGHEAVLAADGAGALARAADPSLRLR
                     AAIVDLHLPGSMPGHEVI"
     gene            complement(305..1165)
                     /gene="istB"
                     /locus_tag="VHK28_06780"
     CDS             complement(305..1165)
                     /gene="istB"
                     /locus_tag="VHK28_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015497784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX2141968.1"
                     /translation="MSARAIDVHALPAMLTALRLPSFHRHWQALAERADQEGWPAARF
                     LAALAEIELAERDTRRIRRHLLEANLPAGKTLATFDFKAIPSIPRARIEALAAGDWLE
                     TGANLIAIGNSGAGKTHLLSAIAHALVEAGRRVLYTRTTDLVQKLQAARRDLALEQAL
                     ARLDRFDLVILDDIAYAQKDQAETSVIFELIARRYENRSLAIAANVPFSAWDRLFPDK
                     AMAVAAIDRLVHHATILEMNVDSYRRRTAASRRRKADGSATTSADNLDAGQHEADAGN
                     LTDNREDITP"
     gene            complement(1162..2670)
                     /gene="istA"
                     /locus_tag="VHK28_06785"
     CDS             complement(1162..2670)
                     /gene="istA"
                     /locus_tag="VHK28_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX2141969.1"
                     /translation="MPGKPVTDQQARAYMQDRPRHSQRAAAARAGFSERTARRIEADP
                     RLPSQRQAERGRTVPDPLAAVWEPVLLPILENDPAVQAITLLRHLQMTDPDAFPDDRV
                     RRTLERRVRDWRALHGAERDIIFRQVPEPGRMALSDFTDAAELAVTIAGQPLAHRLYH
                     FVLAYSGWEHVAVVLGGESFTALAENLQNALWALGGVPQEHRTDSLSAAYRNLDRAAA
                     EDVTRRYEAFCAHYDMAASRNNPGEAHENGAVEAHNRHLKAALDQALILRGSRDFASL
                     ADWRRFVDQLVARRNQRREAALRIEAAALRRLPPRRTTDFTEVVARVTRTGGFLVHGI
                     FYSAPSRLIGQRLRVHVYDDRIEAWLGSTQVVSHPRLRARQDGRRAHCIDYRHVIHAL
                     RRKPQALAASVYRDGLFPRGEYAAAWTALSEVLPRREACRRMVDLLWLAHDEACEAAL
                     ASLISAELAAGHLPDAATLKRRLAPRQRDLPADVPVALTALAQFDTLLEAQA"
BASE COUNT          384 a          922 c         1175 g          445 t
ORIGIN      
        1 gatgacctcg tgtcccggca tgctgccggg gaggtgcagg tccactatcg cggcccgcag
       61 gcgcaggctc ggatcggcgg ctcgggccag ggcaccggcg ccatcggccg ccagcacggc
      121 ctcatggccc gcctcgctca agacctgctc aagctccagc gccagcagtg cctcatcctc
      181 gaccacgaga atgaccgacc gtgtccgcca tgcggctggc gcgctctgct ctctgtagcg
      241 cgtcaaccaa gttggccggg accgacaacc aagatggctg gtgctggccg atgcagttgg
      301 tggttcaggg ggtgatgtcc tccctgttgt cggtgaggtt gccggcgtca gcctcgtgtt
      361 ggccggcatc gaggttgtcg gcggaggttg tcgccgagcc gtcggccttt ctccggcggc
      421 tggctgcggt gcggcggcga tagctgtcga cgttcatctc gaggatggtg gcgtggtgaa
      481 ccaggcggtc gatggcggcg accgccatgg ccttgtccgg gaagaggcga tcccaggcac
      541 tgaacgggac gttggccgcg attgcgaggc tgcggttctc gtagcggcgg gcaatgagct
      601 cgaagatgac gctggtttcc gcctggtcct tctgggcgta ggcgatgtcg tcgaggatga
      661 cgaggtcgaa gcggtcgagc ctggccaggg cctgctcgag ggccagatcg cgccgggcgg
      721 cttgcagctt ttgcacgagg tcagtggtgc gggtgtagag gacgcggcgt ccggcctcga
      781 cgagggcgtg ggcaatggcg ctgaggaggt gggtcttgcc ggcgcccgag ttgccgatgg
      841 cgatgagatt ggcgccggtc tcgagccagt cgccggcggc aagtgcctcg atgcgggcgc
      901 gcgggatgct ggggatggcc ttgaagtcga aggtggccag ggtcttgccg gcgggcaggt
      961 tggcttcgag gaggtgacgg cggatccgcc gggtgtcgcg ctcggcgagt tcgatctcgg
     1021 ccagggcggc gaggaagcgg gcggcgggcc agccctcctg atcggcgcgt tcggcgagcg
     1081 cctgccagtg gcggtggaag ctgggcaggc gcagcgccgt cagcatggcg ggcagggcat
     1141 ggacgtcgat ggcgcgggcg ctcatgcctg cgcctccagc agggtgtcga actgcgccag
     1201 ggcggtgagc gcgacgggca cgtccgcggg caggtcgcgc tggcgcggcg ccaggcggcg
     1261 cttgagggta gccgcatccg gcaggtgccc ggcggccagc tcggcgctga tcaggctggc
     1321 gagcgcggcc tcgcaggcct cgtcatgggc gagccagagg aggtcgacca tgcggcggca
     1381 ggcctcgcgc cggggcagca cctcggacag cgcggtccag gcggcggcgt actcgccgcg
     1441 ggggaagagg ccgtcgcggt agaccgaggc ggcgagcgcc tgcggcttgc ggcgcagggc
     1501 gtggatgacg tgacggtagt cgatgcaatg ggcccggcgc ccgtcctggc gggcccgtag
     1561 ccggggatgg ctgaccacct gggtgctgcc gagccaggcc tcgatgcggt catcgtagac
     1621 atggacgcgt aggcgctggc cgatcagccg cgagggggcg ctgtagaaga tgccgtgcac
     1681 caggaagccg ccggtgcggg tgacccgggc gaccacctcg gtgaagtcgg tggtgcggcg
     1741 gggtggcaga cgccgtaggg cggccgcctc gatccggagc gcggcctcgc ggcgctggtt
     1801 gcgccgagcg acgagctggt caacgaagcg gcgccagtcg gcgaggctgg cgaagtcgcg
     1861 cgagccgcgc aggatcaggg cctggtcgag ggcggccttg aggtggcggt tgtgggcctc
     1921 gaccgcgccg ttctcgtgcg cctcgccggg gttgttgcgg ctagccgcca tgtcgtagtg
     1981 ggcgcagaag gcctcgtagc ggcgggtgac atcctcggcc gccgcgcggt cgaggttgcg
     2041 gtaggccgcc gagaggctgt cggtccggtg ctcctgcggg acgccgccca gggcccagag
     2101 ggcgttctgc aggttctcgg ccagggcggt gaaactctcg ccgccgagca cgacggcgac
     2161 atgctcccag ccgctgtagg ccaggacgaa gtgatagagc cgatgggcca agggctggcc
     2221 ggcaatggtg acggcgagtt ccgcggcgtc ggtgaagtcc gacagcgcca tccggccggg
     2281 ctcgggtacc tggcggaaga tgatgtcgcg ctccgcgccg tgcagggcgc gccagtcccg
     2341 cacccgacgc tccagggtcc gccggacgcg gtcgtcgggg aaggcgtccg ggtcggtcat
     2401 ctgcagatgg cgcagcaggg tgattgcctg caccgccggg tcgttctcca ggattggcag
     2461 cagcaccggc tcccagaccg cggccagcgg gtcggggacg gtgcggccgc gctcagcctg
     2521 gcgctgggat ggtaggcggg gatcggcctc gatccggcgg gcggtgcgct cactgaagcc
     2581 cgcgcgggcg gcggcggcgc gctggctatg acgcggtcgg tcttgcatgt aggctctcgc
     2641 ttgctggtcg gtgacgggtt tgcccggcac gtcgaggtct ccgtttcgtc acagagatcc
     2701 tcctcgcgcc ggcccgcagc ggccagcacc ccacccccat cccggatcga gacggtcgtg
     2761 ccggtggctc cagcctccgg gcgggctacg cccgccctac gcctggagcc acccggacaa
     2821 cttgcctgac ggtgccggtc acgatggttg tcgcagaaca gctcggtgac atagaccaca
     2881 cccaggtcgg gccagcggcg ccgcgcctcc ccggctagcg cgaggc
//
LOCUS       DATACA010000331         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2532103, whole genome shotgun sequence.
ACCESSION   DATACA010000331 DATACA010000000
VERSION     DATACA010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532103"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VHK28_06790"
     CDS             complement(<1..331)
                     /locus_tag="VHK28_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX2141970.1"
                     /translation="MALAATGAAAAPRNFVTPLNGENEVPDRETRARGVAIFQLSPDG
                     ETMSYKLNASNIENVFMAHIHIQPVGQVIGEDNGPIAVWLFETTMPNDQAPVGGGRHD
                     GVLVSGSF"
     gene            524..1243
                     /locus_tag="VHK28_06795"
     CDS             524..1243
                     /locus_tag="VHK28_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2141971.1"
                     /translation="MATVLLVDDDPKIRDLLRLYVEREGHRALFAADGETALDTALRT
                     RPDLVLLDVMLPGLDGFEVCRRLRDVSDVPILLLTARSGEGDKVVGLDMGADDYVVKP
                     FSPRELMARVRAHLRRRRLESDAEEPVLVSNGLEVDPNAVEARLKGDALDLTPFEFRI
                     LRALMRRPGRVFSRDDLIDAIHGGDDPGIIDRTIDVHLGRLRRKLGDDPSAPRYIGTV
                     RTVGYKFVGPVERREPAAAGG"
     gene            1250..>1726
                     /locus_tag="VHK28_06800"
     CDS             1250..>1726
                     /locus_tag="VHK28_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX2141972.1"
                     /translation="MPTLRLRLIGLFVLLALAVAMVMILAVQRFGSEQIMHLAMEAGA
                     SEAEAQAMFDQYVGQVLLIGAGVGVVLGGLAAWWLLRRVLRPLERLTDATRAIAAGDF
                     AARVPDAPDPELQRLADAFNQMAATLERVEQLRRSLVEDVAHELRTPLTSLQGYLEA"
BASE COUNT          243 a          590 c          600 g          293 t
ORIGIN      
        1 tgaagctgcc ggagaccagc acgccgtcgt ggcgaccgcc tccgaccgga gcttggtcat
       61 tgggcatcgt ggtctcgaac agccagacgg cgatggggcc gttgtcctcg cctatgacct
      121 ggccgactgg ctggatgtgg atatgggcca tgaacacgtt ctcgatgttc gaggcgttca
      181 gtttgtagct catcgtctcc ccatcgggac tgagctggaa gatggccacg cctcgtgcac
      241 gagtttcgcg atcggggact tcgttttcgc cgtttagcgg cgtcacgaag ttgcggggag
      301 cggccgcggc acctgtggcg gcgagggcca ccagcagcaa cgaggcggca gcaagggcga
      361 ggagcagtct ccgggacatt cgcgtcgttc ctcctgctgg ggacggttac acgctcgggc
      421 agctcagcca gttgatacgc ggccggttgc acttcgtgcc gggacgcacc ggccagcggc
      481 cacttcatcg gtgcttcatg cgtgggtcat actctccggc gccatggcca ccgttctgct
      541 ggtcgacgac gaccccaaga tccgcgacct gctgcgtctc tacgtggagc gcgagggcca
      601 ccgcgccctg ttcgccgccg acggtgagac ggccctggac acggccctgc gcacgagacc
      661 cgacctcgtg ctgctggacg tcatgcttcc cgggctggac ggcttcgagg tctgccgtcg
      721 gctgcgggac gtctcggacg tgccgatcct gctgctcacc gcgcggagcg gcgagggcga
      781 caaggtcgtt ggactggaca tgggggcgga tgactacgtg gtcaagccct tctcccccag
      841 ggagctgatg gcgcgcgtgc gtgcgcacct gcgacggcgc aggctggagt ccgatgcgga
      901 ggagccggtc ctcgtctcca acgggctcga ggtcgacccg aacgccgtcg aggcacgcct
      961 aaagggtgat gccctcgacc tcacgccgtt cgagttccgc atcctgcggg cgttgatgcg
     1021 ccgtccgggg cgggtcttca gccgcgacga cctgatcgac gccatccacg gcggcgacga
     1081 tccgggaatc atcgatcgca ccatcgacgt gcacctggga cgcctgcggc gcaagctcgg
     1141 agacgacccg agtgctcccc gctacatcgg cacggtgcgg acggtgggct acaagttcgt
     1201 cggtcccgtc gagcgccgcg agcccgccgc cgcaggcggc tgagcagcca tgcccaccct
     1261 gcggctgcgg ctcatcggtc tgttcgtcct gctggccctc gccgtggcca tggtcatgat
     1321 cctggccgtc cagcgcttcg gctccgagca gatcatgcac ctggccatgg aggccggagc
     1381 ctccgaggcc gaggcgcagg ccatgttcga ccagtacgtc ggccaggtgc tgctgatagg
     1441 cgccggtgtc ggagtcgtgc tcggcggcct ggccgcctgg tggctgctgc gccgcgtgct
     1501 tcgtccgctc gagcgcctga ccgatgccac ccgcgccatc gccgccggcg acttcgcggc
     1561 ccgcgtgccc gacgcgccag accccgagct ccagcggctg gccgatgcat tcaaccagat
     1621 ggccgccacg ctcgagcgcg tggagcagct gcgtcggtcg ttggtcgagg acgtggccca
     1681 cgagctgcgc accccgctga cctcgctgca ggggtacctg gaggcc
//
LOCUS       DATACA010000332         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_845776, whole genome shotgun sequence.
ACCESSION   DATACA010000332 DATACA010000000
VERSION     DATACA010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845776"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VHK28_06805"
     CDS             complement(<1..795)
                     /locus_tag="VHK28_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX2141973.1"
                     /translation="MDTDQQLTLRDVPGISVGHATDREGMTGCTVVLCPEGAFAGVDV
                     RGSAPGTRETDLLRPTALVERIHGVCLAGGSAFGLAAADGVMRYLAERGIGFETGVRP
                     VPIVPAAILFDLAVGSPDAVPGPDDGYAACLAAERGEGEVEGRLGAGTGATVAKLAGA
                     EHAIPAGVGSAGMRLPDGSTLAALVVNNALGNVIDHHGELLAGTPIDPEELSAGVGNT
                     VLVVLATDATLDRAECRKLAELAHDGLARSISPAHTLLDGDVAFALS"
     gene            complement(774..1328)
                     /locus_tag="VHK28_06810"
     CDS             complement(774..1328)
                     /locus_tag="VHK28_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2141974.1"
                     /translation="MEEADRLIRSERLELVLLGRDQLERLAAGDRARLASELQAAVPD
                     EWVADVRGLAALRARQLDERPADAPWLLRAIVRTDPAPRAVIGYLNFHSGPNEAGMVE
                     IGYTLLPSARGRGYAIEAVRGAFDWASRAHDVHRFRASVSPDNERSLNLIGKLGFVQT
                     GEQWDPEDGRELVFELDRWTPTSS"
BASE COUNT          238 a          619 c          559 g          214 t
ORIGIN      
        1 ggatagcgcg aacgccacgt ccccgtccag cagggtgtgg gccggcgaga tggaacgagc
       61 cagcccgtca tgcgccagct cggccagctt gcggcactcg gcgcggtcca gcgtggcgtc
      121 ggtggcgagc accaccagga cagtgttgcc cacgccggcg ctgagctcct cgggatcgat
      181 gggcgttccg gccagcaact caccgtggtg gtcgatcacg ttgcccagcg cgttgttcac
      241 caccagggcg gccagcgtgc tgccgtccgg cagccgcatc cccgcggaac ccacacctgc
      301 cgggatagca tgctcggcgc cggccagctt ggccaccgtc gcccccgtgc cggccccgag
      361 ccgcccctcc acctcgccct ctccgcgctc ggcggcgagg caggcggcgt agccgtcatc
      421 cggtccgggc accgcgtccg gggaaccgac ggccaggtcg aacaggatcg ccgccgggac
      481 gatgggcacc ggccgcacgc cggtctcgaa cccgatgccg cgctcggcga ggtagcgcat
      541 gacgccgtcg gccgccgcca gcccgaaggc ggagccgccg gccaggcata ccccgtggat
      601 ccgctccacc agtgccgtcg gccgcaggag gtcggtctcg cgcgtccccg gcgccgaacc
      661 acggacgtcc acgccggcga acgccccctc gggacagagc accaccgtgc agccggtcat
      721 gccctcgcgg tcggtggcgt ggccgaccga gatgccgggc acgtcgcgca gggtcagctg
      781 ctggtcggtg tccatcggtc caattcgaag acgagctcgc ggccgtcctc cgggtcccac
      841 tgctcgccgg tctgcacgaa gccgagcttg ccgatcaggt tgagcgaccg ctcgttgtcc
      901 ggcgacacgc tggcccggaa ccgatgcacg tcgtgcgcgc ggctggccca gtcgaatgcg
      961 ccgcgcaccg cctcgatggc atagccccgt ccacgcgccg agggcagcag cgtgtagccg
     1021 atctcgacca tgccggcctc attcgggccg gagtggaagt tgaggtaacc gatcaccgca
     1081 cgcggagccg ggtccgtgcg cacgatcgcg cgcagcaacc agggagcgtc ggcggggcgc
     1141 tcgtccagct gccgggcgcg gagggcggcg aggccacgca catcggcaac ccactcgtcg
     1201 ggtaccgccg cctgcagctc ggaggccagc cgcgcccggt cgccagccgc taggcgctcg
     1261 agctggtcgc ggccgagcag caccagctcc aggcgctccg agcgaatcag ccggtctgcc
     1321 tcctccaccc cgcgatccta gcgcctcgtg accggttccc cggttgggtg gcggcgatat
     1381 tccgggcgaa attgaagccc gcacgtagct aaaccggggg gtggctgcca gtccagcagg
     1441 ttcccggcgg acagccggta cgccagcttg ggcagccggc tccgcgctgc gtgtcagtct
     1501 gccgatacag cgggcaggtc ccagcacaaa gcgacaagtt cttcgcccct cacgctctcg
     1561 cctgcccgat agaacggcag atcagcacgt ggcggcgccc tcccgcgaaa tcctgcccgc
     1621 tgcatcggcc
//
LOCUS       DATACA010000333         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_443664, whole genome shotgun sequence.
ACCESSION   DATACA010000333 DATACA010000000
VERSION     DATACA010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443664"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..120)
                     /locus_tag="VHK28_06815"
                     /pseudo
     CDS             complement(1..120)
                     /locus_tag="VHK28_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(154..1334)
                     /locus_tag="VHK28_06820"
                     /pseudo
     CDS             complement(154..1334)
                     /locus_tag="VHK28_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612169.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          243 a          573 c          519 g          254 t
ORIGIN      
        1 cagcacgcag ccgaaccggg cgcctgcggc caggacccgg tcgatctcct ccagtgcgat
       61 ccgccacttc gggcggtagc cgacggcctc aggcacacct gccgcggcac gccggcctgt
      121 accggtccac gattttgaga caccgctctg cgcttacgcg gccataggca tcgggctgat
      181 ctgatcggcg cggacttgag cgggcgtctt gtagccgtgc cgctcgatga tccaggtttg
      241 gttgtaggtt cgcttgaacg cgatgagtgc cagccgcagc tcctcgacgg tcgcgaagtg
      301 gcggacccac agcaggttct ccttcagggt ccggatgaag cgctcggcac agccattgcc
      361 ttctggctcg cgcacgaatg ccggtgagct ctcgatacca aggaagcgga tctcggcctg
      421 gaagtcgtgg ctgacgtact ggctcccgtg atcatggcgc agctggaggc cgacggcgat
      481 gccgttggca aacgcgccaa agcgctcgcg caccgcctgc ttgaccggct cgagcgcctc
      541 aaagcgattg gcgctgcgac tggcatggac accaacgcat tcggccgagc agtggtcgac
      601 ggcgatgaag acggcagctt gaccctcgcc ggtcatcacc gaggtcagat cgctgcccca
      661 catcgtatcg acccgatggg tcgtgatcgt gccgtcgtgg gccttcgagc cacgtggccg
      721 gccggcacgt tgatgcgcca gcaggccgtg catccgcatg agccgaagaa cacgacggcg
      781 gctggtacgg atgccggcga agcgcagccg cgcccacagc ttgcgatggc cctcgccatg
      841 gaatgggctg gccgtcagaa gctgtcggat cgccaccacc aggtcctcgt ccgccctggc
      901 cccgagcggg ccggggcgcc tgcgctcaac caccttggtt gggcggcggt ggcgatagag
      961 ggtcgcccgt gagacgcccc agatgcgcgt gacccgcagg aggccgtaga tcctgcccgc
     1021 ggagggcgag acctgccggc tcatcgtctc gacctcctgc gccccaaagg gcggtgcgtc
     1081 tccaggcgct cgatcttcgc ctccagaagc tcggtcgtca tcgtcagctc gccgaccttg
     1141 gcctgcagcc gtccgatctc ggtatcccgc gcatcggccg gccggctctt cagcgacgtc
     1201 tcacccccgg ccaggaaccg gtcgcgccag ccgctcagct cagcagcggt cacagccagg
     1261 tcgcgcgaga ccagctccag gtcctcgccc cgcagcaggc ggagcaccgc ctcctgcttg
     1321 cgtcgtgccg acatccgccg ccgcggctcg ggcgtgggtc cgtctccacc agcgtggtgg
     1381 gcgcgctccg ccgggcccac ccccacccct gcagcctcgt ccatcctcca cctccttgca
     1441 gccgcctcct gcaaagcggt gtctcaacga actgtaggcc gggggactgc aaggttgccg
     1501 gcacgacggc cgcatcaggc tcggaaggag cggtctcgag caccgccttg gtcagctcct
     1561 cgacgaacag cggcacgctg tcggtccgc
//
LOCUS       DATACA010000334         8258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2291980, whole genome shotgun sequence.
ACCESSION   DATACA010000334 DATACA010000000
VERSION     DATACA010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291980"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VHK28_06825"
     CDS             <1..603
                     /locus_tag="VHK28_06825"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX2141975.1"
                     /translation="RSMSQPPPARGRPGGRRRPRDEPREPPDVPAAIEIAARLLATRP
                     RSRWEVQRRLRRAGAQDAVISAALERLAALGYVDDTEFVRWWQEQRDRHAPRGRRMIA
                     AELRQRGVPRDVLDGLDEDAEERTARLGEPDADLPGTEEERADVALERHLRGRPLPED
                     RAGLQRIGMFLVRRGFDPEAARGAIRRRQQERDEPDATEE"
     gene            661..1170
                     /locus_tag="VHK28_06830"
     CDS             661..1170
                     /locus_tag="VHK28_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX2141976.1"
                     /translation="MSTNEARGGHPLAGTTQPQSVDEYIASLPLDRREAVARVREVVN
                     RNLPDGYQEGMSHGMIGWYVPLDRFPDTYNGEPLGLAGLANQKNYMSLYLNSVYGDPR
                     IEEWFRERYAASGKKLNMGKSCVRFRSVDDLPLEVIGETIARADLDSFLGHYRAARGS
                     SRKTRAAKA"
     gene            1209..2156
                     /locus_tag="VHK28_06835"
     CDS             1209..2156
                     /locus_tag="VHK28_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2141977.1"
                     /translation="MTEPQQGLSYEEILALARTAEDAGLESFFRSDHYTSFPGPEGEP
                     TTDAWTTLGGLARETERIGLGVLVSPVTFRMPGSFAKVVATADEMSGGRVEVGMGAGW
                     NDDEHAQHGIGFPSLGERFDMLEEALAIVHGLWTEADGWSFSGDHWNVRGALFRARPE
                     RNGRRHPPIILGGSGGPRLARLVAQFADEFNLVSASPEKAREAYARVDEACTAIGRDP
                     TEVTRSAMTGVLIAETQAELRDRVRAQLAFTAAGDGAEADAWLADRRRRWILGTLDEA
                     GERVAALAEAGTQRIMLQDFLPRDLDQVRLMGRLAAQAS"
     gene            2377..3912
                     /gene="rny"
                     /locus_tag="VHK28_06840"
     CDS             2377..3912
                     /gene="rny"
                     /locus_tag="VHK28_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX2141978.1"
                     /translation="MTNETLVTLGVAFAALIVGVVLGYLARRYLAASAVKHAEAYAER
                     LVAEARAKQKEIVLEGKDDALKIQRAAEEEAREKRADLQRQERLLLDRSESLDRKLEA
                     LERREATFDERQREVDAERGRLAELQQRQLVELERVSGLTVAEARQGLIQQIVDEAQA
                     EAQHRVREIERRASEEGEDRARRILTTVMQRIAADHTSEATVTVVNLPSEEMKGRIIG
                     REGRNIRALEQATGVDLIIDDTPEAVVLSGFDPVRREVARMALTKLISDGRIHPGRIE
                     ETVAKCRAEIEVVMRQAGEQAAYDAGVPGLHPELIKLIGRLKYRTSYGQNVLDHCVET
                     ARLSGLLAAEIGANVNESKKGGLLHDIGKAIDHEGEGPHALIGGEVAKRYGVNAVVCN
                     AIAAHHQEVEQESVEATVVQIADAISASRPGARGESLDNYIKRLDDLQQIALSFPGVE
                     RCYAIQAGREIRILVRPEDVDDLASSRLARDVVRRIEEQLQYPGQIKVTVIRETRAVD
                     YAR"
     gene            3909..4718
                     /locus_tag="VHK28_06845"
     CDS             3909..4718
                     /locus_tag="VHK28_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX2141979.1"
                     /translation="MTDRPAVVRCMMIGDIIGKPGRQAVTAQLAALRRELDVDMVIAN
                     GENMAAGMGLTPSLAEELLGRGVDVVTSGNHIWDKREVYPYLDTGRPVLRPINYPDDA
                     PGRGWLVHHLPDGDQVAVINAMGRVYMNQLDSPFVALDALLDGAAEPLPPIRIVDFHC
                     ELTSEKNAMGWFLDGRVTAVLGTHTHVPTADARVLPKGTAYISDVGMTGPRDSIIGFS
                     LESVLPRFLTHLPTRFAVGEGPVSLNAVVITAERATGRAIGIEQVQRLIEV"
     gene            4736..5671
                     /gene="miaA"
                     /locus_tag="VHK28_06850"
     CDS             4736..5671
                     /gene="miaA"
                     /locus_tag="VHK28_06850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX2141980.1"
                     /translation="MAPPRAPLAAIVGPTASGKTDLALALARRLPVEIVVADSRQVYR
                     GMDVGTAKPDADARAAVPHHLLDLVDPDQPITVHSWVTRARAVVPQIAGRGRVPLLVG
                     GTGLYVSALVDGYRLAAGPASPQLRRELAAALEAEGLDAMVARLGDLDPEAAALTDPR
                     NPRRVLRALERAAVASGSTSPAAEPWAGRLALLGLSRPADVLRQRIAERTAAMFAGGL
                     LDEARQILAAGYDPALAPLSGHGYREAIAVVTGEWPLERAIETTERHTRQYAKRQMTW
                     FRRDPRIVWLTAGDRPADEPALVARAEELLRALLA"
     gene            complement(5687..7330)
                     /locus_tag="VHK28_06855"
     CDS             complement(5687..7330)
                     /locus_tag="VHK28_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX2141981.1"
                     /translation="MDAATEPRETRAPANEALAGLGLRLVLALGVCALAGIATERLLV
                     ESIPSPALRGLLSVLFASLGVGTAAYLTVITPLARSRADLHVRYEAALADALQDPLTE
                     LGNHRAFQEELERQVEAAQRYEVPLSLVLIDLDEFKTINDRLGHAAGDRTLARFGKLV
                     TTSVRRPDRPFRVGGDEFAILLPHTDAEGARIVARRLLATTLAPPLRTAEGRDEPISF
                     SAGISELPGMATSRAQLYSQADAALYAAKRSGRPEVVVFDPTAETAMEPTGAGAAVAE
                     VIARGQLRPVYQPIVELNTLTVLGMEGLIRPVEPAPFSDPGSLFAAAEASGHLVALDL
                     SCVETIVAGARHLADDLFLSVNLSPATVEASEFSAAALISVLARHRFPPDRLVVELTE
                     RQPLNDPARVRAKLDACRQAGIRFAADDLGAGNAGLRLLSELAFDLVKVDLSLIQRSA
                     SSEASSAVVESVVSFAARTGAIVIGEGIERAEQVEQLTALGVRVGQGFLLGRPAPLPA
                     PVPASEGVRPPVRLAPPVPVPAGGATMAAWRQSIGLPVG"
     gene            7473..>8258
                     /gene="hflX"
                     /locus_tag="VHK28_06860"
     CDS             7473..>8258
                     /gene="hflX"
                     /locus_tag="VHK28_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX2141982.1"
                     /translation="MSERSRLIDLTAPEERAFLIALDDGRADGWSAARSLDELAALAE
                     TAGATVVGRSAQRRAAPDPVWYFGRGRAAELADEKAMTGFNLLIVDDELAPNQQRNLE
                     KLVDCKVLDRSALIIDIFARHAKTREGRLQVELAALEYHLPRLTRMWTHLSRTAGGIG
                     TRGPGESQLETDRRLIREKIRKVRSELRDVSRHRATTARQRDRTKIPTVALVGYTNAG
                     KSTLLNAMSDADVFAADMLFATLDPTSRAVELPSGQRVILTDTV"
BASE COUNT         1259 a         2934 c         3015 g         1050 t
ORIGIN      
        1 cgctcgatga gccaaccgcc gcctgctcgg ggccgcccgg gcggtcgccg ccggccgcgc
       61 gacgagccgc gcgagccgcc cgacgtcccc gccgccatcg agatcgccgc gcgcttactg
      121 gccacgcgcc cgcgcagccg ctgggaggtc cagcgccggc tgcggcgcgc cggagcccag
      181 gacgcggtca tcagcgccgc cctggagcga ctggcggcgc tcggctacgt ggacgacacg
      241 gagttcgtgc gctggtggca ggagcagcga gatcgccacg ccccgcgcgg gcgtcgcatg
      301 atcgccgccg agctgcgcca gcgcggcgtg ccgcgggacg tgctggacgg cctcgacgag
      361 gatgcggagg agcggacggc ccgtctcggc gagccggacg cggatctgcc cgggacggag
      421 gaggagcgcg cggacgtggc cctggagcgc cacctgcgcg gccggccgtt gccggaggac
      481 cgcgcgggcc tgcagcgcat cggcatgttc ctggtgcgtc gcggcttcga cccggaagcg
      541 gcaaggggcg ccatcagacg ccggcagcag gagcgggacg agccggacgc gacggaggag
      601 taggcggagc cggacgacgg gggcgcccga gcatcggtcc cagcatcgcg ctaccgtgac
      661 gtgagcacga acgaagcgcg tggagggcat cctttggccg gcaccaccca gccccagagc
      721 gtggacgagt acatcgcctc gctacctctt gaccgccgcg aggcggtggc ccgcgtccgc
      781 gaggtggtca accgcaacct gcccgatggc taccaggagg gcatgtccca cggcatgatc
      841 ggctggtatg tcccgttgga ccgcttcccg gatacgtaca acggggagcc gttggggctc
      901 gccggcctgg cgaaccagaa gaactacatg agcctctacc tcaactcggt atacggcgac
      961 ccgcgcattg aggagtggtt ccgtgagcgg tacgccgcca gcggcaagaa gctgaacatg
     1021 ggcaagagct gcgtccgctt ccgcagcgtt gacgacctgc cgctggaggt catcggcgag
     1081 acgatcgccc gcgccgatct cgacagcttc ctgggccact accgcgcagc ccggggttcg
     1141 tcgcgcaaga cccgcgcggc gaaagcctga gccggcggtt cggagcccag caaatgcggt
     1201 tcgcgctgat gaccgagccc cagcaggggc tgagctacga agagatcctg gccctggcgc
     1261 gcacggccga ggacgccggc ctggagtcct tcttccgatc ggaccactac accagcttcc
     1321 cgggaccaga gggcgagccg accaccgacg cgtggaccac gctcggggga ttggcccgcg
     1381 agacggaacg catcggcctc ggcgtgctgg tctcgccggt caccttccgc atgcccggct
     1441 cgttcgccaa ggtggtggcc accgctgacg agatgagcgg tggccgggtg gaggtgggga
     1501 tgggcgccgg ctggaacgac gatgagcatg cgcagcacgg catcggcttc ccgtcgcttg
     1561 gggagcggtt cgacatgctc gaggaggcgc tggccatcgt ccacgggttg tggacggagg
     1621 cagacggctg gagcttctca ggtgaccact ggaacgtccg cggcgccctg ttccgcgccc
     1681 gcccggagcg caacggccgc cgccacccgc ccatcatcct gggcgggagc ggcggtccac
     1741 gcctggcccg gctggtggcc cagttcgcgg acgagttcaa cctcgtctcc gcctcgccgg
     1801 agaaggcacg cgaggcctat gcccgcgtgg atgaggcctg cacggccatc ggccgcgatc
     1861 cgaccgaggt gacccgctcg gccatgaccg gcgtgctgat cgccgagacg caggccgagc
     1921 tgcgcgatcg cgttcgcgca cagctggcct tcacggccgc gggagacggt gccgaggccg
     1981 atgcctggct ggcggaccgt cgccggcgct ggatcctggg cacgctcgac gaggcggggg
     2041 agcgggtggc ggccctggcg gaggccggta cccagcgcat catgctccag gacttcctgc
     2101 cgcgcgacct cgaccaggtt cggctgatgg gacgtctggc cgcccaggcg agctgagcgc
     2161 gtaacccggc ctcggcggcg atgctcccac gagaaagggc cgtccggagt gggtccaggc
     2221 gagttgcggt acactcgccg aacggcttct gcgatcgccg gccacgaccg gccgcgctga
     2281 ccagcgaggc cgcatgaagc gagatccagc accagctcgc cattcccggc gagcaggaac
     2341 gaacgcccat cagcgggctc cagcacggag gagccgatga cgaacgagac gctcgtcacc
     2401 ctcggggtgg cgttcgcggc gctgatcgtc ggcgtcgtgc tcggctacct ggcgcggcgc
     2461 tacctggccg ccagcgccgt gaagcacgcc gaggcgtacg ccgagcgcct ggtcgccgag
     2521 gcgcgcgcca agcagaagga gatcgtcctc gaaggcaagg acgacgcgct gaagattcag
     2581 cgcgcggccg aggaggaggc gcgcgagaag cgcgccgacc ttcagcgcca ggagcggctg
     2641 ctcctggatc ggtcggagag cctggaccgc aagctggagg cgctcgagcg ccgcgaggcg
     2701 accttcgacg agcgccagcg ggaggtggac gccgagcgcg ggcggctggc ggagctccag
     2761 cagcgccagc tggtggagct ggaacgcgtc agcggcctga ccgtcgcgga ggcgaggcag
     2821 ggcctgatcc agcagatcgt cgacgaggcg caggcagagg cgcagcaccg cgtgcgggag
     2881 atcgagcgac gtgcatccga ggagggcgag gaccgggcgc ggcgcatcct gaccacggtg
     2941 atgcagcgca tcgccgccga ccacacttcc gaggccacgg tgaccgttgt gaacctgccc
     3001 tcggaggaga tgaagggccg catcattggt cgcgagggcc gcaacatccg cgcgctggag
     3061 caggccaccg gggtggatct catcatcgac gacacgcccg aggcggtggt gctctccggc
     3121 ttcgacccgg tgcgccggga agtggcgcgc atggcgctca ccaagctcat cagcgacggg
     3181 cgcatccatc ccgggcggat cgaggagacg gtggccaagt gccgcgccga gatcgaggtc
     3241 gtcatgcgcc aggccggcga gcaggctgcc tacgatgccg gcgtgccggg gctgcaccct
     3301 gagctcatca agctcatcgg tcgtttgaag taccgcacca gctacggcca gaacgtgctg
     3361 gaccactgcg tcgaaaccgc tcgcctgtcg gggctgctgg cagcggagat cggcgccaac
     3421 gtcaacgaga gcaagaaggg cggcctgctg cacgacatcg ggaaggccat cgaccacgag
     3481 ggcgagggcc cgcacgccct gatcggcggc gaggtggcca agcgctacgg cgtcaacgcc
     3541 gtggtgtgca acgcgatcgc cgcccaccac caggaggtcg agcaggagtc ggtcgaggcc
     3601 acggtagtgc agatcgcgga tgccatcagc gcctcccggc cgggggcccg cggggagagc
     3661 ctggacaact acatcaagcg cctcgacgac ctgcagcaga tcgcgctcag cttccccggc
     3721 gtggaacgct gctacgccat ccaggccggg cgcgagatcc gcatcctggt gcggcccgag
     3781 gacgtggacg accttgcctc ctcccgcctg gcgcgcgacg tggtgcgcag gatcgaggag
     3841 cagctccagt accccggcca gatcaaggtg acggtcattc gcgagacgcg ggccgtcgac
     3901 tacgcccgat gaccgaccgc cccgccgtgg tgcggtgcat gatgatcggt gacatcatcg
     3961 gcaagccggg acgacaggcg gtcaccgccc agctggccgc cctgcgccgg gagctggacg
     4021 tcgacatggt gatcgccaac ggcgagaaca tggcggccgg catgggcctg acccccagcc
     4081 tggccgagga gctcctgggc cgcggcgtcg acgtggtgac cagcggcaac cacatctggg
     4141 acaagcgcga ggtctacccc tacctggaca ccggccggcc cgtgctgcgg cccatcaact
     4201 atcccgacga cgcgcccggc cgcggctggc tggttcacca cctgccggac ggcgaccagg
     4261 tggcggtcat caacgccatg ggccgggtct acatgaacca gctcgactcg ccgttcgtcg
     4321 ccctggacgc actcctggac ggcgccgccg agccgctgcc gccgatccgc atcgtcgact
     4381 tccactgcga gctgacctcc gagaagaacg ccatgggctg gttcctggat ggccgggtga
     4441 ccgccgtgct gggcacccac acccacgtgc cgaccgccga cgcccgggtc ctgcccaagg
     4501 gcacggccta catcagcgac gtgggcatga ccgggccgcg cgactcgatc atcggcttca
     4561 gcctggagag cgtcctgccc cgctttctga cccacctgcc cacgcggttc gcggttggcg
     4621 agggcccggt gtcactcaac gcggtggtca tcaccgccga gcgggccacc ggccgggcga
     4681 tcggcatcga gcaggtccag cggctgatcg aagtctgacc cggcagccgg gaccgatggc
     4741 tccgccccgg gcgcccctcg cggccatcgt ggggccaacc gcctccggca agaccgatct
     4801 ggcgctggcg ctggcgcgcc ggctcccggt ggagatcgtg gtcgccgact cgcgccaggt
     4861 ctaccgcggc atggatgtcg ggaccgccaa gcccgatgcc gacgctcgtg ccgccgtgcc
     4921 tcaccacctg ctggacctcg tcgaccccga ccaaccgatc accgtccact cctgggtgac
     4981 gcgcgcacgc gcggtcgtgc cccagatcgc gggacgcggc cgcgtcccgc tgctggtggg
     5041 cggcaccggc ctctacgtct cggcgttggt cgacggctac cggctcgctg ccggacctgc
     5101 ctccccgcag ctgcgccgcg agctggccgc ggcactggag gccgagggac tggatgccat
     5161 ggtcgcgcgg ctgggcgatc tcgacccgga ggcggcagcg ctcaccgacc cgcgcaaccc
     5221 acgccgcgtg ctgcgcgcgc tggaacgagc ggccgtcgca tcgggcagca ccagcccggc
     5281 cgccgagccc tgggcaggga ggctggcgct gctggggctg agccgccccg ccgatgtcct
     5341 ccgccagcgg atcgcggagc gcaccgccgc catgttcgcc ggtgggctgc tggacgaggc
     5401 gcgccagatc ctggccgccg ggtacgaccc ggcgctcgca ccgctcagcg gccacggcta
     5461 ccgtgaggcg atcgccgtgg tcaccgggga gtggcccctg gagcgggcca tcgagaccac
     5521 cgagcgccac acccgccagt acgcgaagcg ccagatgacc tggttccgtc gcgacccacg
     5581 gatcgtgtgg ctgacggccg gggatcgccc ggccgatgag ccggccctcg tcgcccgggc
     5641 cgaggagctg ctgcgcgcgc tccttgcctg aggcccggtc gtgaggctag ccgaccggca
     5701 gtccgatcga ctggcgccag gcggccatcg ttgcgccgcc ggccgggacg ggaaccggtg
     5761 gcgccaggcg cacgggcggg cgcacgccct cggacgctgg tacgggtgcc ggcaggggag
     5821 cggggcggcc gagcaggaat ccctgtccca cgcgaacgcc gagggcggtc agctgctcca
     5881 cctgctcggc ccgctcgatg ccctcgccga tgacgatggc ccccgtgcgt gccgcgaacg
     5941 agacgaccga ttcgaccacg gcgctcgagg cctcgctcga cgcgctgcgc tggatcaggc
     6001 tcaggtcgac cttgaccagg tcgaaggcca gctcgctgag gaggcgcagt ccggcgttgc
     6061 ctgcgcccag gtcatcagcc gcgaagcgga ttcctgcctg gcggcaggca tccagcttgg
     6121 cgcgcaccct ggccgggtcg ttgagcggct gccgctcggt gagctccacc accaggcgat
     6181 ccggcgggaa gcggtgacgg gccagcacgc tgatcaaggc cgccgcgctg aactcggagg
     6241 cttcgaccgt cgccggcgac aggttgaccg acaggaacag gtcgtcggcc aggtgcctgg
     6301 cgccggccac gatcgtctcc acgcagctca agtcgagggc caccaggtgg ccgctggcct
     6361 cggcggcggc gaacagcgag ccggggtcgc tgaagggagc cggttccacg gggcggatca
     6421 gcccctccat gccgagcacg gtcagggtgt tgagctccac gatcggctgg tataccgggc
     6481 gcagctggcc gcgggcgatc acctcggcca cggccgctcc cgcccccgtc ggttccatgg
     6541 ctgtctcggc cgtgggatcg aagaccacga cctcggggcg gccggagcgc ttggccgcgt
     6601 agagggccgc gtctgcctgg ctgtagagct gggcgcggct ggtggccatg cccggcagct
     6661 ccgagatgcc cgccgagaag gagatcggct cgtcgcgccc ttcggcggtg cgcaacggcg
     6721 gagcgagggt ggtggccagc agccggcggg ccacgatgcg cgccccctcg gcgtcggtat
     6781 gcggcagcag gatggcaaac tcgtcgccac cgactcggaa ggggcggtcc gggcggcgca
     6841 ccgaggtggt gaccagcttg ccgaaacggg ccagcgtgcg gtcgcccgcg gcgtgcccca
     6901 gccggtcgtt gatggtcttg aactcgtcga ggtcgatcag caccagcgag agcggcacct
     6961 cgtagcgttg cgcggcctcg acctgacgct cgagctcctc ctgaaaggcc cgatggttgc
     7021 ccagctcggt gagcgggtcc tggagcgcgt cggccagggc cgcctcgtac cggacgtgaa
     7081 ggtcggcccg ggagcgcgcc agcggggtga tgacggtcag gtacgcggcg gtgcccactc
     7141 cgagcgaggc gaacagcacg ctcagcaggc cgcgcagggc gggggagggg atcgactcga
     7201 ccagcagccg ctcggtggcg atgccggcca gggcgcacac cccgagggcc agcaccaggc
     7261 gcaggccgag gcccgccagc gcttcgttcg ccggcgcccg ggtctctcgc ggctccgtgg
     7321 ctgcgtccac gttgcgccct ccagcgcccc cctgggattc gtgctcggag aggtatccca
     7381 cacggggtcc ccgcatgcac tagccggaaa gtaccgacgg ccgggccgcg ttgccggtcg
     7441 cgggcccggc atcggctacg attgagcggc agatgtccga acgttcacgc ctgatcgacc
     7501 tcaccgcacc cgaggagcgc gccttcctga tcgccctcga cgacggccgc gccgacggct
     7561 ggtcggcggc ccgcagcctg gacgagctgg ccgcgttggc tgagacggcc ggcgccacgg
     7621 tggtcggccg cagcgcgcag cgccgagccg ctcccgaccc ggtgtggtac ttcggcaggg
     7681 gccgcgcggc cgagctggcg gacgagaagg cgatgaccgg cttcaacctg ctgatcgtgg
     7741 acgacgagct ggcccccaac cagcagcgca acctggagaa gctcgtcgat tgcaaggtgc
     7801 tggaccgctc ggcgctgatc atcgacatct tcgcccgtca cgcgaagacc cgcgagggtc
     7861 ggctgcaggt cgagctggcc gcgctggagt accacctgcc gcggctgacc cgaatgtgga
     7921 cccacctgtc gcgcaccgcc ggcggcatcg gcactcgcgg acccggtgag agccagctgg
     7981 aaactgaccg ccggctgatc cgcgagaaga tccgcaaggt ccgctccgag ctgcgcgacg
     8041 tgagccgcca ccgagccacc accgcgcgcc agcgggaccg cacgaagatt ccgaccgtcg
     8101 ccctggtcgg ctacaccaac gccggcaaga gcacgctgct caacgccatg agcgacgcgg
     8161 acgtgttcgc cgccgacatg ctgttcgcca ccctcgaccc caccagccgg gcggtcgagc
     8221 tgccatcggg ccagcgcgtg atcctgaccg ataccgtg
//
LOCUS       DATACA010000335         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_283270, whole genome shotgun sequence.
ACCESSION   DATACA010000335 DATACA010000000
VERSION     DATACA010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283270"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..687)
                     /locus_tag="VHK28_06865"
     CDS             complement(16..687)
                     /locus_tag="VHK28_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2141983.1"
                     /translation="MDTETVSFEEFQECLRHLELINRCTLAYRPTLAWLDRLARRRGT
                     GRPLSILDVGFGHGDMLRRIAAWADRSGLPVHLAGVDLNPWSARAASLATPAGLPVRY
                     EVGDVFEVRGDRPIDVVVSSLFAHHLADDDLVRFLRWMDARASAGWFVNDLHRHPLPY
                     HAVRVAARLLPVSRMVRHDAPLSVTRGFTRRDWSTLLRRSGVDRDRVAVEWVFPFRYG
                     VGCLK"
     gene            complement(723..1262)
                     /locus_tag="VHK28_06870"
     CDS             complement(723..1262)
                     /locus_tag="VHK28_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX2141984.1"
                     /translation="MHPAPAGDRGSGGAPDLHGLRRRVRRHPGQRRTRGAADPGLRGR
                     RHPGQRRPDHLARRRAGLRHAPVRRRPGTIARGLPAAMDGLRNRLGIDDISLWAVHPG
                     GRSVLDAVEQSLGLPPEALAASREVLRRYGNMSSPTVMFVLKALMRDAPVSRAGVAIA
                     FGPGLTAETMVFHGAGRRV"
     gene            complement(1196..1804)
                     /locus_tag="VHK28_06875"
                     /pseudo
     CDS             complement(1196..1804)
                     /locus_tag="VHK28_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
BASE COUNT          305 a          674 c          697 g          246 t
ORIGIN      
        1 cgtccggccc ggccctcatt tcaggcatcc cacgccgtag cggaacggga agacccactc
       61 caccgcgacg cggtcgcggt ccacgccgct ccgccgcagg agggtgctcc aatcccgtcg
      121 cgtgaacccg cgcgtcaccg agagcggcgc atcgtgccgg accatgcggc tgaccggcag
      181 cagccgcgcg gcgacgcgga cggcgtgata gggcaacggg tgccgatgca ggtcgttgac
      241 gaaccatccg gccgaggccc gggcgtccat ccagcgcagg aagcggacga ggtcgtcatc
      301 ggccaagtgg tgggcgaaga gcgaactcac gaccacgtcg atcggccggt cgccgcgcac
      361 ctcgaacacg tcgcctacct cgtagcgcac cggcaagccg gccggcgtgg cgagtgaggc
      421 cgcccgcgcc gaccaggggt tcaggtccac gcccgccagg tgcaccggca agccgctgcg
      481 gtccgcccag gcggcgatcc ggcgcaacat gtcgccgtgg ccgaacccga cgtccaggat
      541 cgagagggga cggcccgttc cacgccgccg cgccaggcgg tccagccaag cgagggtcgg
      601 ccggtaagcc agcgtgcagc ggttgatgag ctccaggtgc cggaggcatt cctggaattc
      661 ctcgaaggac accgtctcgg tgtccatcaa ctcggccgcg cggctccgcc gggtgaggcc
      721 ggtcacaccc gccgcccggc gccgtggaac accatggtct cggccgtcag ccccggcccg
      781 aaggcgatgg cgacgccggc ccggcttacc ggcgcgtccc gcatgagcgc cttcaggacg
      841 aacatcacgg tgggcgagga catgttgccg taccggcgca acacctcccg cgatgcggcc
      901 agcgcctccg ggggcaggcc caaggactgt tccaccgcgt ccagcacgga ccggccgccc
      961 ggatgcaccg cccagaggga gatgtcgtcg atccccaggc ggttgcgcag cccatccatg
     1021 gcggccggca ggccgcgcgc gatggtgccg ggacggcgcc ggacaggcgc atgtcgaagc
     1081 cctgcccgcc gacgcgccag gtgatctggt cgtcgctgtc cgggatgacg gcggcctcga
     1141 agcccaggat ctgcagcccc acgggttcgg cgctgaccag ggtggcggcg cacccgtcgg
     1201 cgaagaccat gaaggtcagg agctcctcca gatcctcggt ctcctgcagg tgcagggtgc
     1261 acagctcgac gttgaccatc agcaccttgg ccgccggctc ggagcgcacg atgtggtggg
     1321 cgagcttcag gccgttgatg gccgcgtagc agcccatgaa acccaccacc gtgcgctcga
     1381 cccgatcgtc caggcgctgg cggcgcatga tctccaggtc gatgcccggc gcatgcagcc
     1441 cggtgcaggt ggtgacgatc aggtgggtgg ggcgctccgc ctcggccagg ttcagtgccg
     1501 ccacgcccgc cagcgccagg tccggcgcgt ggcgctcgta gagggccatg cgcgtgcggg
     1561 tatcggggaa ggcatcgggc gcaaacaggc ccccgctgtc cagccggtcc gggctggggt
     1621 ccggctcgag cacggaatag cggtggtcga tctgcgagcg ttccgccatc cggcgaaaga
     1681 tgcgtcgcag ccgctcactg ggaagcaggc gcggcacgtg ctccaggaac ttgcggtgca
     1741 tgtcgtgccg cggcacggcg gtggcgatgc ggttgatgta ggcggcaggc attccaagct
     1801 ccatggcccg ctgcgggtga atggaccaag ctgagctggg tggccctcgg cgtcacggca
     1861 caccgaagag ggcgagcagg agcagcgtca gcaccgccga gacggcgatc gagcctaggc
     1921 gg
//
LOCUS       DATACA010000336         4126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1648437, whole genome shotgun sequence.
ACCESSION   DATACA010000336 DATACA010000000
VERSION     DATACA010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648437"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VHK28_06880"
     CDS             complement(<1..538)
                     /locus_tag="VHK28_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2141985.1"
                     /translation="MTTEAVEPLRLEGLAKSYGSVQAVRGIDLAMRRGEVLGFVGPNG
                     AGKSTTIRCILDLLRPTAGRALLFERDPREHGVELRERVAYVPGELRLPDRLTARQFL
                     RSIGRLRRGFQAARLAELAERFKLDLDRPMSNLSSGNRRKVSVLSAFLTDAELLILDE
                     PTSGLDPLMQHEFLELVRE"
     gene            578..1645
                     /locus_tag="VHK28_06885"
     CDS             578..1645
                     /locus_tag="VHK28_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX2141986.1"
                     /translation="MKLEGRIRVVDAHAGGEPGRVIVGGVTGVPGASMLAKRDHLWRE
                     GDRLRRLVLREPRGYPALCANVVLPPTHPDADAGFVIMEQAEYPAMSGSNTICTATVL
                     ISEGMVPVREPLTELVLEAPAGLIRVRAEVRDGLARSITFENVPAFAVRLDAAVEVPS
                     LGTVVVDVAWGGMFYALTDAAALGFRITPDEGRDLVRVGEMIKAAAREQIPVVHPEQP
                     ELAGITIMEWTGPPTVAGTHGRNTVVVSSGELDWSRAESFTGVLDRSPCGTGTCARMA
                     VLHTRGQLGLGEDFVHEGILGTTWTGRLLSQTRVGPYPAVVPQLTGSAWITGRADLSV
                     DDEDPFPDGFTVGDIWGLTPD"
     gene            1860..2372
                     /locus_tag="VHK28_06890"
     CDS             1860..2372
                     /locus_tag="VHK28_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141987.1"
                     /translation="MRIRLAATAACLLLAGCAPAVSAPPPPSGTSIPSPPATSVLTPG
                     ESSTAAGLRDLQDLLPADFGGVEAHTFPVGQDMLARLAAELGVEDDDVQVAFASDHGP
                     AFIQMFAMRVVGSTADQVADALTRTAYPAEAQPRVSSLRLADRDLAVIRDEALATQRG
                     TFYVLSLPEQ"
     gene            complement(2449..3204)
                     /locus_tag="VHK28_06895"
     CDS             complement(2449..3204)
                     /locus_tag="VHK28_06895"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019069544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX2141988.1"
                     /translation="MNTNLEGRVALVTGAARGIGQAAARELAAHGAAVMITDVIDNEG
                     TAFADELSGAGQRVAYRHLDVTSEEDWMSVVAAAVEQFGRLDILVNNAGIGTMADVEQ
                     ETSKGWDRVIDINQKGVWLGMRAAIPELRKAGGGSIINVSSIFGAVGGFGASVAYHAS
                     KGAVRLMTKNAALRYATENIRVNSLHPAFIDTPLIADLKGTDVEQAILASTPMGRLGR
                     SEEVGSVIAFLASDGASYMTGSEVYVDGGWTAR"
     gene            complement(3218..>4126)
                     /locus_tag="VHK28_06900"
     CDS             complement(3218..>4126)
                     /locus_tag="VHK28_06900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF044548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX2141989.1"
                     /translation="IAIDEARHTVGLQGGRELGWDQLILATGSRIVPEEIEHFDREAH
                     HFYGADAALRLRHALDAFDGGRIVIGIAGMPYKCPPAPLEVAFLVDHELRQRGLRDRS
                     EMHYCSPIGRAFTIESVSEMATPILADKAIELHTFFNVEAIDPGRKVVQSLEGEELAY
                     DLLILVPPHRGAQLVIDAGLAPASGWLPTDKHTLEVRGRPNVHALGDATDLPLSKAGS
                     TAHFEAPVVAERVAAAVQGREPHGKHAEYEGKVMCFFEVGDGKGTLLQFDYDHPPKPP
                     RPNRLWHLGKLAFNRAYWATVPRGRI"
BASE COUNT          605 a         1416 c         1444 g          661 t
ORIGIN      
        1 gttcccgcac cagctccagg aactcgtgct gcatcagtgg atcgaggccc gaggtcggct
       61 cgtccaggat gagcagctcc gcatcggtca ggaacgcgct caggaccgat accttgcgcc
      121 ggttcccgct ggacaggttg ctcatcggcc ggtccaggtc gagcttgaaa cgctcggcca
      181 gctcggccag ccgggctgcc tggaacccgc ggcgcaggcg gccgatggac cggaggaact
      241 ggcgcgcggt caggcggtcg ggcagccgca gctcgccggg cacgtatgcc acccgctcgc
      301 gcagctccac gccgtgctca cggggatccc gctcgaagag cagggcgcgg cccgcggtcg
      361 ggcggaggag gtcgaggatg cagcggatgg tcgtcgactt gccggcgccg ttgggaccaa
      421 cgaagcccag cacctctccg cgacgcatgg ccaggtcgat gccgcgcacg gcctgcaccg
      481 agccatagct cttggccagg ccctcgaggc gcaggggctc gactgcctcg gtcgtcatgg
      541 gccccgagcg tacacgaggg catggcacca tcgggcggtg aaactggaag ggcggatccg
      601 ggtcgtggac gcgcacgccg ggggcgagcc gggacgggtc atcgtcggcg gcgtgaccgg
      661 cgtgccgggc gccagcatgc tggccaagcg cgaccacctg tggcgggaag gtgatcggct
      721 gcgccgcctg gtccttcggg agccgcgcgg ctatcccgcg ctgtgcgcca acgtggtgct
      781 gccgcccacc cacccggacg ccgatgccgg cttcgtgatc atggagcagg ccgagtaccc
      841 ggccatgtcg gggtcgaaca ccatctgcac ggccacggtc ctgatctcgg agggcatggt
      901 cccggtgcgc gagcccctga ccgagctcgt gctcgaggcc cctgccgggc tgatccgcgt
      961 ccgagccgag gtgcgagacg gcctcgcccg ctccatcacc ttcgagaacg tgccggcctt
     1021 tgccgttcgg ttggacgctg cggttgaggt gccctccctt ggcaccgtgg tggtcgacgt
     1081 ggcctggggc ggaatgttct acgccctgac cgatgctgcc gccctgggct tccgcatcac
     1141 ccccgacgag ggccgcgacc tggtgcgggt gggcgagatg atcaaggccg ccgcgcgcga
     1201 gcagatcccg gtcgtccatc ccgagcagcc cgagctggcg ggcatcacca tcatggagtg
     1261 gaccggcccg ccgacggtcg ccggtacgca cggccgcaac acggtggtcg tctcctccgg
     1321 agagctggac tggtcgcggg cggagtcgtt cacgggcgta ctggatcgct cgccgtgcgg
     1381 gacgggcacc tgcgcccgca tggccgtcct ccacacccgt ggccagctgg gcctgggcga
     1441 agacttcgtc cacgagggga tcctgggcac gacctggacg gggcgtctcc tgtctcaaac
     1501 gcgcgtaggc ccctaccccg ccgtggtccc gcagctgacc ggatcggcct ggatcaccgg
     1561 gcgcgctgat ctgtcggtgg acgacgagga tcccttcccg gatggcttca cggtcgggga
     1621 catctgggga ctgacgcccg actgagcatg gctgcacgac acatcgggcg gaatttcacc
     1681 tgggggcacc gcgacgtgtt gttctgccga tctgtcgggc aaccgtgagc gtgacggggc
     1741 gagaaattgc cgcaccctgt gtagcgctgc ccgatgtagc ggcggacgga cacgccagag
     1801 cggcctctgg tgaagtcctg catcaaatat cggccgtgcc gcgcggcgta ttgggtggca
     1861 tgaggattcg gctcgccgcc accgccgcct gcttgctgct cgccggctgc gcgccggcgg
     1921 tgagcgcgcc tccgccgcca tccggcacat cgatcccctc gccgccggcg acttcggtcc
     1981 tcacgccggg cgaatcgtcg accgcggctg gcctacgtga cctccaggac ctgctccccg
     2041 ccgacttcgg aggagtcgag gcgcacacct tcccggtcgg ccaggacatg ctcgcacgcc
     2101 tcgcggcaga gctgggcgtg gaggatgacg acgtccaggt ggcgtttgcc agcgaccacg
     2161 gcccggcatt catccagatg ttcgccatgc gggtggtggg gagcacggcc gaccaggtgg
     2221 cggatgccct gacgcgcact gcatacccgg ccgaggcgca gccgcgggtg agctcactgc
     2281 ggctggcgga ccgcgacctc gccgtaattc gtgacgaggc gctggccacg cagcgcggca
     2341 cgttctacgt gctgtcgctg ccggagcagt agtccgtgat cgtggcccag gccttcgcag
     2401 cgccggtggt ggaagcggcg ctcaatgact tgccgtgaag gcggcgagct agcgggcggt
     2461 ccagccgccg tcgacgtaca cctcgctgcc ggtcatgtag gacgcgccgt ccgacgccag
     2521 gaaggcgatc accgatccga cctcctcgga ccgcccgagg cggcccatcg gcgtgctggc
     2581 caggatggcc tgctcgacat cggtcccttt gagatccgcg atcaacggcg tgtcgatgaa
     2641 ggcagggtgg agcgagttga cccggatgtt ctcggttgcg tagcgcagcg ccgcgttctt
     2701 ggtcatcagc cgcacggcgc ctttggaggc gtggtaggcg accgatgcgc cgaagccgcc
     2761 caccgcgccg aagatggacg atacgttgat gatcgagccg ccgcctgcct tgcgcagctc
     2821 cgggatggcg gcccgcatac cgagccagac gcccttctgg ttgatgtcga tcactcggtc
     2881 ccaacctttg gaggtctcct gctcgacatc cgccatggtg ccgatgccgg cgttgttgac
     2941 caggatgtcc agccggccga actgctcgac cgcggcggcc acaaccgaca tccagtcctc
     3001 ctccgaagtg acgtcgaggt gccggtaggc gacccgctgg ccggccccgc tgagctcgtc
     3061 ggcgaaggcg gtgccctcgt tatcgatgac gtcggtgatc atgactgccg caccatgcgc
     3121 cgccagctcg cgcgcagccg cctgaccgat acctcgcgcc gcgccggtga cgagggcgac
     3181 acgaccctcg agattggtgt tcatcggtct cctttcgtca gatccggccc cggggcacgg
     3241 tggcccagta ggcccggttg aacgccagct tgccgaggtg ccagagccga tttggcctgg
     3301 gtggcttcgg ggggtggtcg tagtcgaact gcagtagtgt gcctttgccg tcaccgacct
     3361 cgaagaagca catcaccttg ccctcgtact cggcgtgctt cccgtggggc tcgcggccct
     3421 ggaccgcggc cgccacccgc tcggccacca cgggggcctc gaaatgcgcg gtcgacccgg
     3481 ccttggagag cggcaaatcg gtcgcgtcgc ccagggcatg aacgtttggt cgcccacgta
     3541 cctccagcgt gtgcttgtcg gtcggaagcc agcccgatgc cggcgccagg ccggcatcga
     3601 tgaccagctg tgcgccgcgg tgcggcggta ccaggatgag gaggtcgtat gccagctcct
     3661 ctccctccag gctctgaacc accttgcgcc ccggatcgat ggcctccacg ttgaagaagg
     3721 tgtgcagctc gatcgccttg tcggcgagga tgggagtggc catctcggag acgctctcga
     3781 tggtgaacgc gcgcccgatg gggctgcagt agtgcatctc gctgcgatcg cgcaggccgc
     3841 gctgccgcag ctcgtgatcg accaggaacg ccacctcgag cggggcgggt gggcacttgt
     3901 acggcatgcc ggcaataccg atcacgatgc gcccgccgtc gaaggcgtcc agggcatggc
     3961 gcagccgcag cgctgcatcg gctccataga agtggtgcgc ctcccgatca aaatgctcga
     4021 tctcctccgg cacgatgcgc gaaccggtgg ccaggatgag ctgatcccag ccgagctctc
     4081 ggccgccctg cagcccgacg gtgtgccgtg cctcatcgat ggcaat
//
LOCUS       DATACA010000337         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2211485, whole genome shotgun sequence.
ACCESSION   DATACA010000337 DATACA010000000
VERSION     DATACA010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211485"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VHK28_06905"
     CDS             complement(<1..857)
                     /locus_tag="VHK28_06905"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2141990.1"
                     /translation="MLRILRQRNFALLWTGGLISMIGDWILIVGLPYEVYRRTGSTLA
                     TGAMVLAFLVPSIVLGSVAGVFVDRWDRRRLMIAVNLLLAIAILPLLVIDAVGLWVAY
                     AVLLVTSSLEQVFHPAEGALLPNLLENPDDDLLTANALNGMNNNLARLIGPAVGGAIV
                     ATGGILAVTLVDAVTFLVSAALIWGIRAPNTRAAAVGALEQAAVGAWRRLATEWRDGL
                     RVIGHHPVLRAMLVFFVITRIGEGLTMTLFVPWVTDALNSDAAGYGAVLSTQAIGGFV
                     GAVVIARLGA"
     gene            954..1039
                     /locus_tag="VHK28_06910"
     tRNA            954..1039
                     /locus_tag="VHK28_06910"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:988..990,aa:Tyr,seq:gta)
     gene            1150..1225
                     /locus_tag="VHK28_06915"
     tRNA            1150..1225
                     /locus_tag="VHK28_06915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Thr,seq:ggt)
     gene            1259..2317
                     /locus_tag="VHK28_06920"
     CDS             1259..2317
                     /locus_tag="VHK28_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141991.1"
                     /translation="MDPNVVFPFLSSLLSFIFAVLVADQWLRRRQSYQLVWTVGLLWY
                     GISAGTEFIGGAWGWDEPLYRTWYLVGAFGVAAYLGLGTVYLLRNTRFGYFVAFSVFA
                     GGLFSILSAVARAREGDPTSAGVVPAVLAFATLAAVAIAVATRIHRPLAATVSAVVLV
                     LASVVVTYLTLTVPVSPPGFFLDPATGVPTGEALPSDLRVLTPPFNIAGAFALVFGAF
                     YSAYIFMPKRRVMRVETQLPVVGAVGRAVAVVVNFFASIPGAVRAFGEGRLNSRVPAT
                     LLIALGGFIPGWTSGLNRFGITWSFYLGEFAGVLLIFLGFLVSIEVFSDIRLPFTNVV
                     LARRDRKARSETEASADT"
     gene            2773..2937
                     /gene="rpmG"
                     /locus_tag="VHK28_06925"
     CDS             2773..2937
                     /gene="rpmG"
                     /locus_tag="VHK28_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015022386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX2141992.1"
                     /translation="MAKAENRPVISLACTVCKERTYSTRKNKRNDPGRLELNKFCPRC
                     RQHQLHRETK"
     gene            3017..3092
                     /locus_tag="VHK28_06930"
     tRNA            3017..3092
                     /locus_tag="VHK28_06930"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3050..3052,aa:Trp,seq:cca)
     gene            3139..3321
                     /gene="secE"
                     /locus_tag="VHK28_06935"
     CDS             3139..3321
                     /gene="secE"
                     /locus_tag="VHK28_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX2141993.1"
                     /translation="MIRGIRLYLSEAWSELKKVAWPTRQTVINLTLIVIAVSVAVGAY
                     IAVLDLGLKAAVDQVL"
     gene            3321..3932
                     /gene="nusG"
                     /locus_tag="VHK28_06940"
     CDS             3321..3932
                     /gene="nusG"
                     /locus_tag="VHK28_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX2141994.1"
                     /translation="MTDEQTRTTEATQAARLSREAPLPAAAEGTAKQWYVIHTYSGYE
                     NKVKANLEHRIESMGVEDQIFQVLVPMEEEIEIKNGQRQTVNKKVFPGYVLAEMVMGD
                     ESWYVVRNTPGVTSFVGSGNRPTPLDENEVKKILKQMGVEAPKFKQQFTKGQSVRVKD
                     GPFAEFIGTVDEVNPEKNKVKVLVSIFGRETPVELDFLQIDKL"
     gene            4027..4452
                     /gene="rplK"
                     /locus_tag="VHK28_06945"
     CDS             4027..4452
                     /gene="rplK"
                     /locus_tag="VHK28_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2141995.1"
                     /translation="MAKKVRTVLKVQAPAGSATAAPPVGPALGQHGINIMEFIKAFNE
                     RTANLRGYVVPAEITVYEDRSFTFVTKAPLAAALLKRAAGVESGSAQPNRDKVGSVNQ
                     QQLREIAETKMADLNANSVEQAMKIVAGTARSMGIEVRD"
     gene            4452..5159
                     /gene="rplA"
                     /locus_tag="VHK28_06950"
     CDS             4452..5159
                     /gene="rplA"
                     /locus_tag="VHK28_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX2141996.1"
                     /translation="MARHGRKYREAAALIEAERRYALPEAAELLPRLSTSKFDATVEA
                     HLRLGVDPRHADQLVRGTVVLPHGTGRTTRVIVFAQGEKAQEALRAGADEVGGDDLVK
                     RIDDGWFEFDVAIATPDMMGTVGRLGKKLGPRGLMPNPKSGTVTFDIERAVGEIKSGR
                     IEFKVDRAGIVHAPVGRASFSPDQLTENVATLVDAVNRAKPSGAKGTYMRTLTLAPTM
                     GPGVRIDIPSALAAAAS"
BASE COUNT          898 a         1725 c         1767 g          833 t
ORIGIN      
        1 gccccgagcc gcgcgatgac caccgccccc acgaagccac caatggcctg ggttgacagc
       61 acagcgccgt agcccgccgc gtccgagttg agggcatcgg tcacccacgg aacgaacagc
      121 gtcatggtca acccctcccc gatgcgggtg atcacgaaga agaccagcat cgcgcgcagc
      181 acggggtgat gaccgatgac gcgcaggccg tcgcgccact ctgtggccag ccgccgccag
      241 gcgccgacgg ccgcctgctc cagggccccg actgcggcgg cgcgcgtgtt cggtgcccgg
      301 atgccccaga tgagcgccgc cgagaccagg aaggtaaccg cgtccaccag cgtgacggcc
      361 aggatgccgc cggtcgccac gatcgccccg ccgacggccg gaccgatgag ccgcgccagg
      421 ttgttgttca tgccgttaag ggcgttggcg gtgagcaggt cgtcatcggg gttctcgagc
      481 aggttgggga gcagtgcgcc ctcggccggg tggaagacct gctccaggct gctggtcacc
      541 agcaggaccg cgtaggccac ccacaggccc acggcatcga tcaccagcag cggcaggatg
      601 gcgatcgcca gcagcaggtt gaccgcgatc atcaagcggc gccgatccca tcggtccacg
      661 aagacgccgg ccaccgagcc gagcacgatg gagggcacca ggaaggccag caccatggcg
      721 ccggtggcca gcgtggatcc ggtgcgccgg tacacctcgt agggcaggcc cacgatcagg
      781 atccagtcgc cgatcatcga gatcagcccg ccggtccaca gcagggcgaa gttgcgttgg
      841 cggaggatgc gcagcaccgg cggattatgg gggctgcctc ttgaggccag agccccgcat
      901 cggatatact cttggtccga tcggcatacc accacatgcc ggtcggtttc cgtgggtagg
      961 ttgagcaggt ggtgagctcc gctgactgta gatcagccgt ctatcgactg tgggggttcg
     1021 attccctccc tgcccaccag ccctcccacg cggcacggcg acatcggtcc aggagaatcc
     1081 cggacataga tttaccgaga tagaccgatg gttgccgaga atccagcggt tccggcacaa
     1141 ttccagcgcg cccacgtagc tcagtaggca gagcacttcc ttggtaagga agaggtctcc
     1201 ggttcgagcc cggacgtggg ctccagactc attgcgctgg aggccgtgat cgaagcccgt
     1261 ggatccgaac gtcgtcttcc ccttcctgtc gtccctcctg tcgttcatct tcgccgtgct
     1321 ggtggccgac cagtggctgc gccgccgcca gtcgtaccag ctggtctgga ccgtcgggct
     1381 gctgtggtac gggatcagtg ccgggaccga gttcatcggc ggggcctggg gctgggacga
     1441 gccgctgtac cgcacctggt atttggtcgg cgccttcggg gtggccgcct acctgggcct
     1501 gggcaccgta tacctgctgc gcaacacgcg cttcggctac ttcgtggcct tcagcgtgtt
     1561 cgccgggggc ctgttcagca tcctgtcggc ggtggcccgc gctcgggaag gtgatcccac
     1621 gtctgccggc gtggtgccgg cggtcctggc cttcgccacc ctcgcggcgg tggcaatcgc
     1681 cgtggcgacc cgcatccacc ggcccttggc cgccaccgtc tcggcggtcg tcctggtcct
     1741 ggcctcggtg gtggtgacct acctgaccct gaccgtgccg gtcagcccgc ccggattctt
     1801 cctggacccg gctaccggcg ttcccaccgg cgaggcgctt ccctctgacc tgcgggtcct
     1861 gacgccgccg ttcaacatcg ccggcgcctt cgcgctggtg ttcggcgcgt tctactccgc
     1921 ctacatcttc atgcccaagc ggcgggtgat gcgggtggag acgcagcttc ccgtggtcgg
     1981 cgccgtgggc cgcgcggtgg cggtggtcgt caacttcttc gcgtccatcc cgggcgcggt
     2041 gcgggccttc ggcgaggggc ggcttaattc acgagtgccg gccacgctgc tgatcgcgct
     2101 gggcgggttc attccgggct ggaccagcgg gctgaatcgc ttcggcatca cgtggtcctt
     2161 ctacctgggc gagttcgccg gcgtgctgct catcttcctg gggttcctgg tcagcatcga
     2221 ggtcttcagc gacatccggc tgcccttcac caacgtcgtg ctggcgcgcc gcgaccgcaa
     2281 ggcccgctcc gagaccgagg cgtcggccga cacctaggct gggtgctgcc gcaatcggta
     2341 agcggcgagc tggcggacca cctgcgctcg gtcctcgtat tgggatgctg cccgacacag
     2401 cgggcagtgg ttcacgggaa gtcgtcgccg cgtgctgctc tgccgacata tcgggcagcg
     2461 cgcagggtga agggcgaaga attcgtcggt tcgtgcgtgg ccctgcccga cacatcggcg
     2521 atctgacaca tgagcgggcg tttgcgtgaa ggactgcacg gtataacggc ggctgaggcc
     2581 atgactgctg gcatcgtcgc gggagatcct accgggcctg gcccgggcaa ggcgccaagc
     2641 ccccactggg tcacgttgac atcggtcgta aatcgaccgt acaatcggca ggctttcccc
     2701 cgggattccg accctagtta agcgtggccg gcgccgggtg accgcagcag tttcgacaaa
     2761 ggacatccaa ccgtggccaa ggccgagaac cgtccggtga tctcgctggc gtgcaccgtc
     2821 tgcaaggagc gcacgtacag cacccggaag aacaagagga acgatcccgg gcggctggag
     2881 ctgaacaagt tctgcccccg ctgccggcag catcagctgc accgggagac caagtaggcg
     2941 ggtgaccgcc gtcagccggc tgggcgggcc ttgcgccgcg accgccggag ggcgaaagta
     3001 gacgtaccga gtcggtaggg gtgtagctca actggtagag cactggtctc caaaaccagc
     3061 ggttgcaggt tcgagtcctg tcgcccctgc cacacacatc accatcccca tcccattcca
     3121 ggagcgagct agaacgacgt gatccgcggc attcgcctct acctgagcga ggcatggtcc
     3181 gagctcaaga aggtggcctg gcccacccgt cagacggtga tcaacctgac gttgatcgtg
     3241 atcgccgtca gcgtggcggt gggcgcgtac atcgccgtcc tggaccttgg cctcaaggcc
     3301 gcagtcgacc aggtgctttg atgaccgacg agcagacccg taccaccgag gccacgcagg
     3361 ccgctcgcct ctctcgtgag gcgccgctgc cggctgccgc cgagggcacc gccaagcagt
     3421 ggtacgtgat ccacacctac tccgggtacg agaacaaggt gaaggccaac ctcgagcacc
     3481 gcatcgagtc gatgggtgtc gaggaccaga tcttccaggt gctggtgccc atggaggagg
     3541 agatcgagat caagaacggc cagcggcaga ccgtcaacaa gaaggtcttc cccggctacg
     3601 tcctggccga gatggtgatg ggcgacgagt cctggtacgt ggtgcgcaac acgcctggcg
     3661 tcaccagctt cgtcggttcc ggcaaccgcc cgacgccgct ggatgagaac gaggtcaaga
     3721 agatcctcaa gcagatgggg gtcgaggcgc cgaagttcaa gcagcagttc accaagggcc
     3781 agagcgtccg ggtcaaagat gggcccttcg ccgagttcat cggtaccgtg gacgaggtca
     3841 acccggagaa gaacaaggtc aaggtgctcg tcagcatctt cggccgcgag acgcctgtcg
     3901 agctggactt cctgcagatc gacaagctct agagaaaaga ccgatggagc cgccgcggcg
     3961 gcatcggtcc atccgcaaga gagggaggag cgcgaaccgc tccgtcagta cctcggaggt
     4021 taggacatgg caaagaaggt acgcaccgtc ctcaaggtgc aggcgccggc cggatcggca
     4081 accgccgcgc cgccggtggg tcccgcgctg ggccagcacg ggatcaacat catggagttc
     4141 atcaaggcct tcaatgagcg gaccgccaac ctgcgcggct acgtcgtgcc ggcggagatc
     4201 accgtctacg aggaccgctc cttcaccttc gtcaccaagg cgccgttggc ggccgcgctg
     4261 ctgaagcgcg ctgcgggcgt ggagtccggc tcggcccagc cgaaccgcga caaggtggga
     4321 agcgtcaacc agcagcagct acgcgagatc gccgagacca agatggccga tttgaacgcc
     4381 aactcggttg agcaggccat gaagatcgtg gccggcacgg cgcgcagcat gggcatcgag
     4441 gtgcgcgact gatggccagg cacggacgca agtaccgcga ggcagccgcg ctgatcgagg
     4501 ccgagcgccg ctatgcactg ccagaggcag ccgagctgct cccgcgcctc tccacctcga
     4561 agtttgacgc caccgtggag gcgcacttgc gcctcggcgt ggacccgcgg cacgcggacc
     4621 agctggtgcg cggcaccgtg gtgctgccgc acgggactgg ccgcaccacg cgggtgatcg
     4681 tcttcgccca gggcgagaag gcccaggagg cgctgcgcgc cggtgcggac gaggtgggtg
     4741 gcgacgacct ggtcaagcgc atcgacgacg gctggttcga gttcgacgtg gccatcgcca
     4801 cgcccgacat gatgggcacc gtaggccgcc tcgggaagaa gctcggtcca cgcgggctga
     4861 tgccgaatcc caagtcgggg acggtgacct tcgacatcga gcgggcggtg ggagagatca
     4921 agtccggtcg catcgagttc aaggtggacc gcgccgggat cgtccacgct ccggtcggcc
     4981 gtgccagctt cagtccggac cagctgacgg agaacgtggc cacccttgtg gacgcggtca
     5041 accgcgccaa gccgtccggt gccaagggaa cgtacatgcg gacgctaacc ctggcgccga
     5101 ccatgggccc cggcgtgcgg atcgatattc catcggccct ggccgcagcc gcgtcgtagg
     5161 gcgtttgccg tcgaggcgct gaccggctac catcgtcgac cgtaactgaa gaaccacatc
     5221 cac
//
LOCUS       DATACA010000338         2913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2612541, whole genome shotgun sequence.
ACCESSION   DATACA010000338 DATACA010000000
VERSION     DATACA010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612541"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1707)
                     /locus_tag="VHK28_06955"
     CDS             complement(79..1707)
                     /locus_tag="VHK28_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2141997.1"
                     /translation="MLTTPPPRPLELLVVGGLTIDRFPDGSIAPGGSVLHAARAMWLD
                     GRRVAVVTVAGDEPEARHGLDELRRLGELHLQPAEATIGFGHGEEGGGRRLRLLATGD
                     RVRLPELDEPPAAVLYAPVVDELDPAALDAVPAAARRGAILQGWLRRVAPGEAVHGRP
                     LRDLPADLLARLGEMDLLVASSEDLAAEAATPAAQLDALRQVVGPRPITALTAGTEGV
                     FLDVPGEPISRVRPSRVIVGVSAVGAGDAFAAAMLAALARGVRPLPAAADAGALVAEH
                     LAERSGRATYVLGDVHGMLEPMRVLLRDAGLTDAQDRWSGGRHELWLTGDLVDRGPAG
                     VEVIAMLMRLQHDAASAGGRVASVAGNHELLLLAAREIPDEVAGGPGGTFLSDWLANG
                     GQHSDLDALTPEHASWLRRLPAVARVGTVLLVHADATLYASLGASVSEVNERWIEWLA
                     TADPDRWDELLAGLSARRAFLADPNAADRLLARFGGRQIVHGHTPISHLVRAEARQVS
                     APHLYADGRVVAVDAGLAAGAAGFLYRLPAATFS"
     gene            1725..2045
                     /locus_tag="VHK28_06960"
     CDS             1725..2045
                     /locus_tag="VHK28_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2141998.1"
                     /translation="MRLYFYELHEGATDLMTDALLVSDTRHEPEAFAELVRQARTAVL
                     DTFEEDTLVEAIAREMERRHGFTYIGDEKLTASMSVGMEGEDTYLVEQSEDYRSIVAE
                     VERS"
     gene            complement(2055..>2913)
                     /locus_tag="VHK28_06965"
     CDS             complement(2055..>2913)
                     /locus_tag="VHK28_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX2141999.1"
                     /translation="AREGTVQPDVLAQIRNADALLHVARAFEGGDRPADPWRDVEEME
                     LEFAVADLTVVQKRLERLATSGRHGSPSEREQNAQEEELLRRLEPHLSDGLPLRTFSL
                     TDDEEHRLRGYRFLTQKPVLVVLNIDEARLGEAGALEEAGRGRLADPQSDVAALAGKI
                     EAEIAQLPPEDAALFMEDLGIAEPSRGRVVRLTYELLGLFSFFTAGEDECRAWTLRRG
                     ATAVDAAGAIHTDLARGFIRAEVVSFEDLVACGSTAEARKRGLLRSEGKSYAVRDGDV
                     IEVLFNVSR"
BASE COUNT          433 a         1083 c         1031 g          366 t
ORIGIN      
        1 gtcgcggctc cggcgcctga ggacctggac acaggcggcc gagcggcgtc gcgcccgggc
       61 cacccggcgt gccgaacctc agctgaaggt ggccgccggc agccggtaga gaaagccagc
      121 agcgcccgcg gccaggccgg cgtcgaccgc caccactcgg ccgtcagcgt agaggtgcgg
      181 agcactcacc tggcgtgcct cggcgcggac caggtgcgag atcggcgtat gcccgtgcac
      241 aatctgtcga ccgccgaatc gtgccagcag ccgatcagcg gcattcgggt cggcaaggaa
      301 ggcgcgccgc gcgctcaacc cggccagcag ctcgtcccag cggtcgggat ccgcggtcgc
      361 cagccattcg atccagcgct cattcacctc ggagacggac gcgcccaggc tggcgtacag
      421 cgtggcgtcg gcatggacta gcaggacggt ccccacgcgc gcgacggcgg gcagtcgccg
      481 cagccaactg gcgtgttcgg gagtgagggc gtccaggtcc gaatgctgcc cgccattggc
      541 cagccagtcg ctcaggaacg tgccgcccgg gccgccggcc acctcgtcgg ggatctcgcg
      601 cgcggcgagc agcagcagct cgtggttgcc ggccaccgac gccacccggc cacccgccga
      661 cgccgcgtcg tgttgcaggc gcatgagcat ggcgatcacc tcgacgccgg ccgggccccg
      721 atccaccaag tccccggtca gccacagctc gtggcggccg ccgctccacc ggtcctgggc
      781 atcggtcaat cccgcgtcgc gaagcagaac gcgcatcggc tcgagcatgc cgtgcacgtc
      841 gccgagcacg tacgtcgcgc gacccgagcg ctcggccaga tgctcggcca ccagcgcgcc
      901 ggcgtcagcc gccgccggca ggggccgcac tccacgggca agggcggcca gcatggcagc
      961 ggcgaaggca tcgcctgccc cgactgcaga gacgccgacg atcacgcgcg agggacggac
     1021 gcggctgatc ggctcccccg gcacgtccag gaagacgccc tcggtgccgg cggtgagggc
     1081 tgtgatgggg cgcggaccaa cgacctggcg cagggcatcc agctgcgcgg cgggcgtggc
     1141 cgcctcggcg gccaggtcct cgctgctcgc caccagcagg tccatctcgc ccaggcgcgc
     1201 cagcaaatcc gccggcaggt cgcgcagcgg gcgaccgtgc accgcttccc cgggggcaac
     1261 acggcggagc cagccctgga ggatggctcc tcgtcgggcc gcagccggca cggcatcgag
     1321 ggctgccggg tccagctcat cgaccacggg cgcgtacagg accgccgccg gcggctcgtc
     1381 cagctctggc agccgcactc gatcgccggt tgccaggagt ctcagtcgcc gccccccgcc
     1441 ctcctcgcca tgcccgaagc cgatcgtggc ctcggccggc tggaggtgca gctcgccgag
     1501 ccggcgcagc tcgtccaggc cgtgccgcgc ttccggctca tcgccggcca cggtaaccac
     1561 cgccactcgc cggccgtcca gccacatggc gcgggcggcg tgcagcacgg agccgccggg
     1621 cgcgatggag ccatcgggga agcggtcgat ggtgagccca ccgaccacca gcagctccag
     1681 ggggcggggc ggcggtgtgg tcagcatcgg tctatcatcg gccgatgcga ctctacttct
     1741 acgagctgca cgagggcgct accgacctga tgaccgacgc cctgctggtc agcgataccc
     1801 gccatgagcc ggaggccttc gccgagctcg tgcgccaggc gcggacggcc gtgctggaca
     1861 ccttcgagga ggacaccctg gtcgaggcca tcgctcgcga gatggagcgg cggcacggct
     1921 tcacgtacat cggcgacgag aagctgaccg ccagcatgag cgtcgggatg gagggcgagg
     1981 acacctatct cgtggagcag tccgaggact accgcagcat cgtggccgag gtggagcgca
     2041 gctgacgccg tcgctcaacg cgagacgttg aacaggactt cgatcacgtc cccgtcgcgc
     2101 accgcgtagc tcttgccctc ggatctcagc aggccgcgct tgcgcgcctc tgcggtggag
     2161 ccgcaggcga cgagatcctc gaacgacacg acttccgcgc gaataaagcc gcgggccagg
     2221 tcggtgtgaa tggcgccggc cgcatcgacc gccgtggcgc cgcggcgcag ggtccaggcc
     2281 cggcactcgt cctccccggc ggtgaagaag ctgaacagcc ccagcagctc gtaggtcagg
     2341 cgcaccaccc ggccgcggct cggctcagcg atccccaggt cctccatgaa gagcgcggcg
     2401 tcctccggcg ggagctgggc gatctcggcc tcgatcttcc cggccagggc ggcgacgtcc
     2461 gactgcggat cggccaggcg cccgcgcccc gcctcctcga gggcgccggc ctcgcccagc
     2521 cgcgcctcgt cgatgttgag caccacgagc acgggcttct gtgtcaggaa gcggtagccg
     2581 cgcaggcggt gttcctcgtc atcggtcagc gagaaggtgc gcagcggcag cccgtccgag
     2641 agatgaggct ccagccggcg cagcagctcc tcctcctggg cgttctgctc gcgctcggag
     2701 ggagatccgt ggcggccgga ggtggccagc cgctcgagcc gcttctggac gacggtcagg
     2761 tcggcgacgg cgaactccag ctccatctcc tcgacgtcgc gccagggatc cgccgggcgg
     2821 tctccgccct cgaaggcacg cgccacgtgc agcagcgcgt ccgcattgcg gatctgtgcc
     2881 agcacgtcgg gctggacggt cccctcccgc gcc
//
LOCUS       DATACA010000339         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_846318, whole genome shotgun sequence.
ACCESSION   DATACA010000339 DATACA010000000
VERSION     DATACA010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846318"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VHK28_06970"
     CDS             complement(<1..344)
                     /locus_tag="VHK28_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142000.1"
                     /translation="MSTAPEQSTTRPGAVRSWKTPVTLAVLAVFALLVFAIGAPGNEV
                     TFRLSDPGDAMVLPPVVIFAPVMGWICAVVMLAAAVLAFVQVRAGRPVPGWLIAVFAV
                     VFVAAFLSWIVG"
     gene            complement(353..1651)
                     /locus_tag="VHK28_06975"
     CDS             complement(353..1651)
                     /locus_tag="VHK28_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142001.1"
                     /translation="MVDTEDGRIAPPPVPATAQSGDLHPGARSSQSVVQRIMSGDALV
                     AVLAVVLSLIAGGILIALTDEDVAQTASYFFARPQDMLLAAWSAASEAYLALFQGSVF
                     NFEGQSFTRMIYPLMQTLTVATPLICAGLGVALAFRAGLFNIGAQGQIIIGATLAAWV
                     GFSLHLPVGIHLLLVIVAGIIGGAIWGGLVGLLKAKTGAHEVILTIMLNYVAIYLVTY
                     FLTTPAFQRPGSSNPISPQLDETAMYPPLFGDAFRLHWGFIVALLATVFVWWLLKRST
                     IGFELRAVGANPVAARTAGISVTKGYVVVMLIAGALAGLAGVAQVAGTEQVLTSGVAA
                     SFGFDAITVALLGRSTPWGTFFAGLLFGAFRAGGVTMQTSTGTSIDIVLVVQSLIVLF
                     IAAPPLVRALFHLPEPGKTKGPKRPKGTESRKPVTTGGSA"
BASE COUNT          321 a          586 c          607 g          263 t
ORIGIN      
        1 ctgccgacga tccagctcag gaaggccgcc acaaatacga cggcgaacac ggcgatcagc
       61 cagcccggaa ccggccggcc ggcccgaacc tgcacgaagg ccaggacggc tgcggcaagc
      121 atgacgaccg cgcagatcca gcccatgacc ggggcgaaga tgacaacggg agggagcacc
      181 atggcatccc ccgggtcgga caggcggaag gtcacttcgt tgccgggcgc cccgatggcg
      241 aagaccagta gcgcgaagac ggccagcaca gccagcgtga ccggggtctt ccagctccgc
      301 actgcgccgg ggcgggtggt gctctgctcg ggcgcggttg acactgccgt gctcatgctg
      361 aacctccggt ggtgacgggc ttgcgggatt cggttccctt ggggcgcttg ggccccttgg
      421 tcttgccggg ttcgggcagg tggaacagcg ccctgaccag cggcggggcc gcgatgaaga
      481 gcacgatcag ggactggacc accagcacaa tgtcgatgct ggtgccggtg ctggtctgca
      541 tggtcacgcc gccggcgcgg aaggcaccga agagcagccc ggcgaagaac gttccccacg
      601 gcgtcgagcg ccccagcagc gccacggtga tcgcgtcgaa gccgaagctg gctgccacgc
      661 cggaggtcag aacctgctcg gtgccggcca cctgggcgac gcctgcgagc cctgccagtg
      721 ccccggcgat cagcatgacg acgacgtagc ccttggtcac gctgatgcct gcggtgcgtg
      781 ccgccaccgg gttcgcgcca accgcgcgca gctcgaagcc gatggtcgac cgcttaagca
      841 gccaccagac gaacaccgta gccagcagcg ccacgatgaa gccccagtgc agccggaagg
      901 cgtcgccgaa cagcgggggg tacatcgccg tttcatccag ttgcgggctg atcgggttgg
      961 acgatcccgg ccgttggaag gcgggggtgg tcaggaagta ggtgaccagg tagatcgcca
     1021 cgtagttgag catgatggtc aggatgactt catgcgcgcc ggtcttcgcc ttgagcagac
     1081 ccaccaggcc gccccagatc gctccgccga taatgccggc cacaatgacc agcagcaggt
     1141 ggatgccgac cggaaggtgc aggctgaagc ccacccaggc cgcaagggtg gcgccgatga
     1201 tgatctggcc ctgggcaccg atgttgaaca ggccggcgcg gaaggccagc gccacaccca
     1261 ggccggcgca gatcagcggc gtagccacgg tcagcgtctg catcagcggg tagatcatcc
     1321 gcgtgaaact ctggccctca aagttgaaga ccgacccttg gaagagcgca aggtaggcct
     1381 cggacgccgc cgaccaagcg gccaggagca tgtcctgcgg gcgcgcgaag aagtagctgg
     1441 ccgtctgggc aacgtcctcg tcggtcagcg cgatcaggat tccgcccgca atcagcgaaa
     1501 gcacgacggc gagcaccgcc accagggcgt cgccgctcat gatccgttgg acaacggact
     1561 gagaactccg cgctccgggg tgcaggtctc cgctttgcgc agtggccggt accggcggcg
     1621 gagctatccg tccgtcctcc gtgtcgacca tggcctcaag acggacctcg gaggcgtgct
     1681 cgtcggtttt cttccgggcg tgcttatgct cgtcctggct catttgtccc cactttcagg
     1741 atcagtgcct gccggctccg tgctttgcgc ttcctcc
//
LOCUS       DATACA010000340         9394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1166645, whole genome shotgun sequence.
ACCESSION   DATACA010000340 DATACA010000000
VERSION     DATACA010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166645"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VHK28_06980"
     CDS             complement(<1..809)
                     /locus_tag="VHK28_06980"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142002.1"
                     /translation="MTSATQTSGTPAPAQPGGGMRVRLSDVRVSFPAPDGRLTALDGL
                     NLDIPAGSFTAVIGPNGCGKSTLLRAVAGLLVPAVGTVMLGDGTAAPRAGDGRVGLAF
                     QQPRLVPWLTTVDNVALPLALRGSVPAERRERALGALERVGLGGAAHLRPAQLSGGMA
                     QRAALARSLIGDPPVLLLDEPFSALDALTREAFDTELQALWQEQRRTIILVTHSVPEA
                     VRLADRVVVMTARPGRVASLVEVQAPHPRPVAPTHPGVAAADRAVRDALSDV"
     gene            complement(1178..1855)
                     /locus_tag="VHK28_06985"
     CDS             complement(1178..1855)
                     /locus_tag="VHK28_06985"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX2142003.1"
                     /translation="MLDFIDQTIIPFFLDLYDAVGYVGVTIAIGLETFVPIIPSEVIV
                     PMAGWKVSQSAADPSVVEPLTGAPWSFLAVLVVATVGAVLGSLAGYLIGAWGGRPLLD
                     RWGRYVHINPDDLDRADAWFARYGDWAVLIARLVPLLRALINYPAGVARMPLGRFLLF
                     STLGSLPWNAGLLLAGYLLGENYEQLYDAVRPFEYLIYAVVIIGGAYILYRWIRGRGG
                     PDEAVVA"
     gene            complement(1879..2700)
                     /locus_tag="VHK28_06990"
     CDS             complement(1879..2700)
                     /locus_tag="VHK28_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2142004.1"
                     /translation="MRRLPDPTSRLLVAADGKDRLGGAVPSPAAAFLLTLIVLPSLFW
                     LVTLKRTTEYTVPEEWLMANFVPAAQSFVRENAPLLEEIIVGISGLGSGWFVAAGFGV
                     LGLLALAKGRRDLAVVLAAGTLAFPIEWALKFFTAIPEISFTQLVGAMFDIGGVGLDD
                     LADFPAGHALRATVFYGLAAFCVARLAPDRRQGLTAYAVALVLIGAISLVRIYLGAHY
                     PMDVLGGWMAGAALLSVIVAVHILNVDERLGAAPLPATDDAPRRVAVPLHDRRDA"
     gene            2761..4278
                     /gene="hutH"
                     /locus_tag="VHK28_06995"
     CDS             2761..4278
                     /gene="hutH"
                     /locus_tag="VHK28_06995"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX2142005.1"
                     /translation="MVRRDELLLSGRDLDVADVVAVARQGRRVAIDAAARDRMRASRA
                     VIERLVSEGAVAYGVTTGFGDLANVRIDPDQTAELQRNIVRSHAAGVGDPLPAEVVRA
                     MLVLRANALAVGLSGVRPELVDLLCGMLNAEVHPLVPSRGSVGASGDLAPLAHLALVV
                     IGEGEAWLDDAGPGPGGEALRRAGLQPMVLEAKEGLALLNGTQLMSALAALSLHDARR
                     LAVAADVIGAMSLEAMLGTAAAFDEKLVGARPHPGQVAVAAHLRGLLAGSEIGASHAR
                     SQHRLQDPYSLRCMPQVHGAVRDALDQLARVLRVEMNAATDNPLVFPSGEVVSGGNFH
                     GEPLALAIDYVKTAMVELASISERRSARLVDAHLSGLPPFLTDEPGLRSGLMIAQYTA
                     ASLVNELKVLVHPASADSIPTSANQEDHVSMGATSALHLRETIDRVELVLAIEALCAA
                     QGLDYRAPMRPGDGVAHAHARLRSMVPRLAEDRPPSGDIAAVRELLRVGELSIDG"
     gene            4271..5011
                     /locus_tag="VHK28_07000"
     CDS             4271..5011
                     /locus_tag="VHK28_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142006.1"
                     /translation="MADLLFSLVEPGTFDDIPAPTRPGARCQTCDYWERLDGAREADD
                     SDAARRLKLSRLMEGTRLAGAYGMLAWRTGSDGDRLAVGWAQFGPISAYPRAQVIRDR
                     YPQLPESPAPWVITCLQVVETDEDRDGAARSLLDEVCEELDRRGIVAVEAYPEGVADP
                     WQPSPGPETAYAASGFQRVAGDDRYPVMRRELGGSSDDVGWGDLLSRTKPADEGDDWP
                     LPLPEGPSEDDLFRLPQKPKRPNPFGDD"
     gene            5059..6117
                     /locus_tag="VHK28_07005"
     CDS             5059..6117
                     /locus_tag="VHK28_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005274359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2142007.1"
                     /translation="MSFRRLPSILLPAAMLVAVTACTDDSPSPTGALGSPQATDFTSP
                     APEARGAPPLTSLAHIVDEVQPSVVSIVREQGEGSGVIWSADGLVVTNEHVVRGTTMV
                     EVAFADGQRVEAEVLATDPRTDLAVVRAERAGLPAATFVEGLPEVGDLAMAMGNPIGF
                     ENSVTAGIISGLHRSIPGAARQAPALVDLIQTDAAISPGNSGGALVNAEGEVVGINVA
                     YLPPSGGAVSIGFAIPAETVRNVIEQLLEDGTAEHAYLGVQPATVTPQIAQQFDLAVE
                     RGVVVLAVGAGTPAAAAGMEPGDVLVSVDGESLETAEELLAVLRGYVPGDEVVLGVVR
                     GGEETEVPVQLGELPAAR"
     gene            6389..8068
                     /gene="hutU"
                     /locus_tag="VHK28_07010"
     CDS             6389..8068
                     /gene="hutU"
                     /locus_tag="VHK28_07010"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX2142008.1"
                     /translation="MTATAPRSGPRPVRAPRGTTLSCRGWQQEAALRMLMNNLDPDVA
                     EDPDQLVVYGGTGRAARSWEALDAIIAELRRLADDETLLVQSGKPVGVFRTTRDAPRV
                     LIANANLVPHWATWEQFRELERAGLTMYGQMTAGSWIYIATQGIIQGTYETFAEAARQ
                     HFDGSLRGTATLTAGLGGMGGAQPLAITMNEGAALVVEVDPARARRRHDAGYVDEMTH
                     DLDEAVAKVDAWRAAGEGRSIALIANAADVEPELVRRNWRPDLATDQTSAHDPLGGYV
                     PGGIGLEEARELRERDPAEYTRRSLASMAEHVRAMLAFQASGSVVFDYGNNIRQHAHD
                     AGVTDAFDFPGFVPAFIRPLFCEGKGPFRWAALSGDPEDILKTDAALMQLFPEDESLY
                     RWLRLAQQKVPFQGLPARICWLGYGERARAGLRINQMVASGELAAPIVIGRDHLDAGS
                     VASPFRETEGMRDGSDAIADWPILNALLNTAAGATWVSFHHGGGVGIGYSLHAGMAIV
                     ADGTPEAAARLERVLTTDPGMGVIRHADAGYQRATEVARERGIRIPMSAEE"
     gene            complement(8073..9185)
                     /locus_tag="VHK28_07015"
     CDS             complement(8073..9185)
                     /locus_tag="VHK28_07015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX2142009.1"
                     /translation="MTDLPRPPVPDRPAMAGFEPDLTVDPGLVNVFDYERRAAERIAA
                     GPLAYFAGGALDERTLNDNRAAFGRRRIVPRVMTDVAEVDTGVEVLGRRWPWPVFVSP
                     TALQRMAHPDGELATARAAAARGITMALSSSASTDMADVAGAGGSRWYQVYLLADPGA
                     RRAMIERAVSLGYEALVLTVDLQRLGRRERDVRVRFQIPDGVGLPNIAAAASVAPDEA
                     ASVPFVERLTWDDLEWLAGFGRPVVVKGVLHPEDAGLAIEHGAAAIQVSNHGGRQLDG
                     AIASLDALPAVVEAVEGRVPVLVDGGVRRGTDALVALALGATAVGIGRPVIWGLAVDG
                     EAGVGRVLDLVTSELEQAMALAGVPSVRALGSDLVV"
BASE COUNT         1344 a         3464 c         3373 g         1213 t
ORIGIN      
        1 acgtcactca acgcgtcgcg aacggcgcga tcggcagccg ccacgcctgg atgggtgggg
       61 gccaccggcc gcgggtgggg agcctgcacc tccaccaggc tggccacccg cccggggcgg
      121 gcggtcatga ccaccacccg gtccgccagg cggaccgcct cggggacgga gtgggtcacc
      181 aggatgatgg tgcgccgctg ctcctgccag agggcctgca gctcggtgtc gaacgcctcg
      241 cgcgtcagcg cgtcgagcgc tgagaacggc tcgtccagga gcagcaccgg tgggtcaccg
      301 atcagcgacc gtgccagtgc cgcgcgctgg gccatccccc cggacagctg cgccgggcgg
      361 aggtgggctg ccccgccgag gcccactcgc tccagggctc ccagggcgcg ttcccgtcgt
      421 tcggccggga cgctgccgcg cagggccagg ggcagcgcca cgttgtcgac ggtggtcagc
      481 caggggacca gccgcggctg ctggaaggcg aggccgaccc gaccatcccc cgctcgtggc
      541 gccgccgtgc cgtcacccag catgaccgtc cccaccgccg gcaccagcag cccggcgacg
      601 gcacgcagca acgtggactt accgcagccg ttggggccga tgacggcggt aaagctcccc
      661 gccgggatgt caaggttcag gccgtcgagg gcggtgagcc gtccgtccgg ggccgggaac
      721 gagacgcgga cgtccgagag gcgcaccctc atgccgccgc ccggctgggc gggagcaggg
      781 gtgccgctcg tttgggtggc gctggtcacg tgtcgctccg gtcgactgct ggccatcgca
      841 cacggggcgc cgccacgagc gcaccaacca cgccagcgcc ccgcgccaca gagggacggg
      901 agggccgatg cgtcggagat acgccttgcc ggtcgtcttc tcccttccgg actgtgaccg
      961 tcggccctgt ctgccgccgc gcctcgcgcg cgccgggtca ggtcggcccc gtcgggctca
     1021 gctgcagcgg tctccgccgc cgcggccctc tggggttcgc gggctggcgc cgaggcgccg
     1081 accgccggtg gggactttca ccccgccccg aagacgatgg tttcgttcag ttggtgtgca
     1141 gcgtcgcacg tcgcggcgtt gccggtcaac cgcctggcta ggcgaccacc gcctcgtccg
     1201 gcccgccacg accccggatc catcggtaca ggatgtacgc cccaccaatg atcacgaccg
     1261 cgtagatcag gtactcgaag ggacggaccg cgtcgtacag ctgctcgtag ttctcgccga
     1321 ggaggtagcc ggcgagcagc agcccggcgt tccagggcag cgagcccagg gtgctgaaca
     1381 gcaggaagcg cccgagcggc atgcgcgcca cgccggcggg gtagttgatc agggcccgca
     1441 gcaggggcac caggcgggcg atcaggaccg cccagtcacc gtacctggcg aaccaggcgt
     1501 cggcccggtc caggtcgtcg gggttgatgt ggacgtagcg gccccaccgg tccagcagcg
     1561 gacggccgcc ccaggcgccg atcaggtagc cggccagcga cccgagcacg gcgcccacgg
     1621 tggccaccac cagtaccgcc aggaagctcc acggggcgcc tgtcagcggc tccaccaccg
     1681 acgggtcggc ggccgactgg ctcaccttcc agccggccat cggcacgatc acctccgagg
     1741 ggatgatcgg cacgaaggtc tccagcccga tggcgatggt gacccccacg tagcccacgg
     1801 cgtcgtagag gtccaggaag aaggggatga tggtctggtc gatgaagtcg agcatgagcg
     1861 ccggattgta cgggcgatct atgcgtcgcg ccggtcgtgc agcggcacgg ccacccggcg
     1921 gggggcgtca tcggtggccg gtagcggtgc cgcgcccagt cgctcgtcga cgttcaggat
     1981 gtggaccgcc acgatcaccg acagcagggc cgcgccggcc atccagccac ccagcacgtc
     2041 catcgggtaa tgcgcgccga ggtagatgcg caccaggctg atggcaccga tgaggaccag
     2101 cgccacggca taggcggtca gcccctggcg gcggtccggg gccagccgtg ccacgcagaa
     2161 cgcagccagg ccgtagaaca cggtggcgcg cagcgcatgg ccggcaggga agtcggccag
     2221 gtcgtccagc ccgaccccac cgatgtcgaa catggcgccc accagctgcg tgaagctgat
     2281 ctccgggatg gcggtgaaga acttgagcgc ccactcgatc gggaaggcga gtgtgccggc
     2341 ggccagcacg accgccaggt cgcgccgtcc cttggccagg gccagcaggc ccagcacgcc
     2401 gaagccggct gccacgaacc agcccgagcc gaggccactg atgcccacga tgatctcctc
     2461 gaggagcggg gcgttctcgc gtacgaagga ctgggcggcc ggcacgaagt tggccatcag
     2521 ccactcctcg ggcacggtgt actcggtggt gcgcttcaag gtcaccagcc agaacaggct
     2581 gggcagcacg atcagggtca gcaggaaggc cgccgccggg ctgggcactg cgccacccag
     2641 ccggtccttg ccgtcggccg ccaccagcag gcggctggtg gggtccggca gtcgtcgcac
     2701 ggcccgtagt atagggacgc ggtctccgcc gaccgcggaa aggagccccc cgaaacggac
     2761 gtggtacggc gcgacgagct gctcctgagc gggcgcgacc tggacgtggc cgacgtggtc
     2821 gccgtggccc gccaaggccg gcgggtggcc atcgatgccg cagcccggga ccgcatgcgt
     2881 gcctctcgcg ccgtcatcga acggctcgtc tccgagggtg cggtcgccta tggggtcacc
     2941 accggattcg gcgatttggc caacgtgcgc atcgaccccg accagaccgc tgagctgcag
     3001 cgcaacatcg tgcgttcgca cgcggcgggc gtgggtgacc cgctgccggc cgaggtggtg
     3061 cgcgccatgc tggtcctgcg cgccaatgcc ctggcggtcg gcctctccgg cgtgcggccc
     3121 gagctcgtcg acctgctgtg cggaatgctg aacgcggagg tccacccgct cgtgccatcg
     3181 cggggcagcg tcggggcatc cggcgacctg gcgccgctcg cgcacctggc gctggtggtc
     3241 atcggtgagg gcgaagcctg gctggacgac gccggcccgg gccccggtgg cgaggcgctg
     3301 cgacgcgccg ggctgcagcc catggtcctg gaagccaagg aggggctcgc cctgctcaac
     3361 ggcacccagc tgatgagcgc gctggccgct ctgtcgctcc acgacgcgcg gcggctggcg
     3421 gtggcggccg acgtgatcgg cgccatgagc ctggaggcca tgctgggcac cgcggccgcc
     3481 ttcgacgaga agctggtcgg cgcccgtccg caccccggcc aggtggcggt ggcggcacac
     3541 ctgcgcggcc tgctggccgg gtcggagatc ggcgcctccc acgcccgcag ccagcaccgg
     3601 ctccaggacc cgtactcgct gcgctgcatg ccccaggtgc acggggccgt tcgcgacgcg
     3661 ctggatcagc tcgcgcgcgt gctgcgggtg gagatgaacg cggccaccga caacccgctc
     3721 gtcttcccca gcggcgaggt ggtctccggc ggcaacttcc acggcgagcc gctggccctg
     3781 gccatcgact acgtcaagac ggccatggtc gagctggcct cgatctccga gcgccgttcg
     3841 gcgcgcctgg tggacgcaca cctgtccggt ctcccaccct tcctgaccga tgagcccggg
     3901 ctgcgctccg ggctgatgat cgcccagtac accgccgcct cactggtgaa cgagctgaag
     3961 gtgctggtcc acccagccag cgcggacagc atcccgacca gcgccaacca ggaggaccac
     4021 gtctccatgg gtgccacctc ggcgctgcac ctgcgcgaga cgatcgaccg cgtcgagctg
     4081 gtgctggcca tcgaggcgct gtgcgcggcg caggggctcg actaccgcgc gccgatgcgg
     4141 ccgggcgacg gcgtggccca cgcccacgct cgcctgcgct ccatggtccc ccgactggcc
     4201 gaggatcgac ctccgtccgg cgacatcgcc gccgtgcgcg agctgctgcg ggtcggggag
     4261 ctctcgatcg atggctgacc tgctgttctc cctggtggag cccggtacgt tcgacgacat
     4321 cccagcgcca acgcgtcccg gagcgcgctg ccagacctgc gactactggg agcgcctgga
     4381 cggagcgcgg gaggcggacg actcggacgc ggcccgtcgc ctcaagctgt cgcgcctgat
     4441 ggaggggacg cggctggccg gtgcctacgg catgctcgcg tggcgcaccg ggtcggacgg
     4501 ggaccgcctg gccgtcggct gggcacagtt cggacccatc tccgcctacc cgcgtgccca
     4561 ggtcattcgt gaccgctacc cgcagctccc cgagtcgccg gcgccatggg tgatcacctg
     4621 cctgcaggtg gtcgagaccg acgaggaccg tgacggcgcc gcccgcagcc tgctcgacga
     4681 ggtctgcgag gagctcgacc ggcgcgggat cgtggccgtg gaggcctacc ccgagggcgt
     4741 cgccgatccc tggcagccct cgccgggacc cgagacggca tacgccgcct ccggcttcca
     4801 gcgcgtcgcc ggagacgacc gctacccggt catgcgccgc gagctgggag gcagctctga
     4861 cgacgtcggg tggggtgacc tgctgtcccg caccaagccg gccgacgagg gtgacgactg
     4921 gccactgccg ctgcccgaag ggccaagcga ggacgacctc ttccggcttc cgcagaagcc
     4981 gaagcgcccc aaccccttcg gggacgactg agcgccgtcc gcgcctggtg gcatcacggc
     5041 tgcacgcacc ggcagcgcat gtcattccgc cgtctcccgt cgatcctgct gccggctgcc
     5101 atgctggtgg ccgtcacggc ctgcaccgat gattcgccat cgccaaccgg agccctgggc
     5161 tcgccgcagg ccaccgactt cacctctccg gcgcccgagg cccgcggcgc gccgccgctg
     5221 acctcgctgg cccacatcgt cgacgaggtg caaccctcgg tggtctccat cgtccgtgag
     5281 caaggcgagg gaagcggggt gatctggtcg gcggatggcc tggtcgtcac caacgagcac
     5341 gtcgtgcgcg gcacgactat ggtggaggtg gcgttcgccg acgggcagcg cgtggaggcc
     5401 gaggtgctgg ccaccgaccc acgcaccgac ctggccgtcg tcagggccga gcgcgccggc
     5461 ctgccggccg cgaccttcgt ggaggggctg ccggaggtgg gcgacctcgc catggccatg
     5521 ggcaacccga tcggcttcga gaacagcgtg acggctggga tcatctccgg gctgcaccgg
     5581 tccatcccgg gcgcggcccg ccaggctccg gcgctggtcg acctcatcca gaccgatgcc
     5641 gccatctcac cgggaaactc aggcggggcg ctggtcaatg ccgaaggaga ggtggtcggc
     5701 atcaacgtgg cctacctccc gccctcgggc ggggcggtga gcatcggctt cgccatcccg
     5761 gctgagaccg tacgcaacgt gatcgagcag ctgctggagg acggaacggc cgagcacgct
     5821 tacctcggcg tccagccggc gacggtgacg ccgcagatcg cgcagcagtt cgacctggcc
     5881 gtggagcgcg gggtcgtcgt gctggccgtc ggagccggca cgccggcagc cgccgccggc
     5941 atggagcccg gtgacgtgct ggtgtccgtg gacggtgagt cgctggagac cgccgaggag
     6001 ctgctggccg tcctgcgcgg ctacgtcccg ggcgacgagg tggtgctcgg cgtcgtccgc
     6061 ggcggggagg agacggaggt gcccgtccag ctcggggagc tgccggccgc gcgctgagct
     6121 ccttcaggtg ccgtgcccct gcgacgcatc ggtccgggcc gatacagcgg gcagttcttc
     6181 acctgaaccg gcggtggcgt gtcagaacgc cgatccatcg ggcagctctc agcacgaacc
     6241 gacatgaact tcgcccctca cggtcaccgc tgccggatac atcgccggaa cggcacgccg
     6301 ggcgacccgc ccgtgaacct ctgcccgata catcgcagcg caaggggagg cagccgccgc
     6361 ttccgctggc cgccgtatca tcaccgccat gacggccacc gcaccccggt ccggcccccg
     6421 ccccgttcgc gcccctcgcg gcaccacgct gtcctgccgc ggctggcagc aggaagcggc
     6481 gctgcgaatg ctgatgaaca acctcgaccc ggacgtggcc gaggaccctg accagctggt
     6541 ggtctacggc ggcacgggtc gcgcggcgcg cagctgggag gccctcgatg ccatcatcgc
     6601 cgagctgcgg cggctggcgg acgacgagac gctcctggtc cagtccggca agccggtggg
     6661 cgtcttccgc accacgcgcg acgcgccgcg ggtgctgatc gccaacgcca acctggtccc
     6721 ccactgggcg acgtgggagc agttccgcga gctggagcgc gccggcctca cgatgtacgg
     6781 ccagatgacc gccggctcgt ggatctacat cgccacccag ggcatcatcc agggcaccta
     6841 cgagaccttc gccgaggccg cgcgccagca cttcgacggc tcgctgcggg ggacggccac
     6901 cctgaccgcg ggcctgggcg gcatgggcgg cgcgcagcca ctggccatca ccatgaacga
     6961 gggcgccgcg ctcgtcgtcg aggtggaccc ggcccgggca cgacgccgcc atgacgccgg
     7021 ctacgtcgac gagatgacgc acgacctcga cgaggccgtc gcgaaggtgg atgcctggcg
     7081 cgccgccggc gagggacggt cgatcgcgct gatcgccaac gccgcggacg tcgagccgga
     7141 gctcgtgcgc cgaaactggc gtcccgacct ggccaccgac cagacctccg cccacgaccc
     7201 gctgggcggc tacgtgccgg gaggcatcgg tctggaggaa gcgcgcgagc tgcgcgagcg
     7261 cgaccccgcg gagtacaccc gccgctccct ggcctccatg gccgagcacg tgcgggccat
     7321 gctcgccttc caggcatcgg gctcggtcgt cttcgattac ggcaacaaca tccggcagca
     7381 cgcccacgac gccggcgtga ccgatgcctt cgacttcccc ggcttcgtgc ccgccttcat
     7441 ccggccgctg ttctgcgagg ggaaggggcc cttccgctgg gccgccctgt cgggcgatcc
     7501 cgaggacatc ctgaagaccg atgcagccct gatgcagctc ttcccggagg acgaatcact
     7561 gtaccgatgg ctgcggctgg cccagcagaa ggtgccgttc cagggccttc cggcccggat
     7621 ctgctggctg ggctacggcg agcgggcgcg cgccgggctg cgcatcaacc agatggtggc
     7681 ctcgggcgag ctggcggcgc cgatcgtgat cgggcgcgac catttggacg cggggtcggt
     7741 ggcctccccc ttccgcgaga cggaggggat gcgcgacggg tcggacgcca tcgccgactg
     7801 gccgatcctc aacgccttgc tgaacacggc cgccggcgcc acctgggtca gcttccacca
     7861 cggcggcggg gtgggcatcg ggtacagcct gcacgccggc atggcgatcg tggccgacgg
     7921 cacgccggag gccgccgccc ggctggagcg cgtgctgacc accgacccgg ggatgggcgt
     7981 catccgccac gcggacgccg gctaccagcg cgcgaccgag gtcgcccgcg agcgcggcat
     8041 ccgcatcccg atgtccgcgg aggaataaag ggtcagacga ccaagtccga gcccagggcg
     8101 cgcactgagg gaaccccggc cagcgccatg gcctgctcca gctcggacgt gaccaggtcc
     8161 agcacccgcc ccacgccggc ctcgccgtcc acggccaggc cccagatgac gggtcgcccg
     8221 atgccgaccg cggtcgcgcc cagcgccagc gccaccagcg catcggtccc gcgtcggacg
     8281 ccgccgtcga cgagaaccgg gacacggccc tccaccgcct cgaccaccgc cggcagggca
     8341 tccaggctgg cgatcgcccc gtccaattgc cgccctccgt ggttgctcac ctggattgcg
     8401 gccgcgccgt gctcgatcgc cagccccgcg tcctcggggt gcagcacgcc cttgaccacc
     8461 accggccggc cgaagccggc cagccactcc aggtcgtccc aggtcagccg ctcgacgaac
     8521 gggaccgatg ccgcctcgtc cggcgccacg ctcgcggcgg cggcgatgtt cggaagcccg
     8581 acgccgtcgg ggatctggaa gcggacgcgg acgtcgcgct cccggcgccc gaggcgttgg
     8641 aggtcgacgg tgagcaccag cgcctcatat cccagcgaga cggcccgttc gatcatggcc
     8701 cgccgcgcgc ccgggtcggc cagcaggtag acctggtacc agcgcgagcc gccggcgcca
     8761 gccacgtccg ccatgtcggt cgacgccgag gacgagagcg ccatggtgat cccgcgggca
     8821 gcagccgccc gggcggtcgc cagctcgccg tcggggtggg ccatgcgctg cagcgcggtg
     8881 ggcgacacga acacgggcca cggccagcgg cggccgagca cctcgacgcc ggtgtcgacc
     8941 tccgccacgt cggtcatcac ccgcggcacg atcctccgcc ggccgaaggc cgcgcggttg
     9001 tcgttcaggg tgcgctcgtc cagggcaccg ccggcgaagt aggccagcgg gccggccgcg
     9061 atgcgctcgg ccgcgcgcct ctcgtagtcg aagacgttga cgagacccgg gtcgacggtg
     9121 aggtccggct cgaagccggc catcgccggc cgatccggta ccggcggccg cggcaggtcg
     9181 gtcatgcgcg aagcgtagcg gcgcgcgtag catcgcggcc atgcccttcg accccgccgc
     9241 gcccctgccc ggcccgcccg acccctggca gccgtcccac atgccgcaac gacgggtggg
     9301 gccgccgttc gcgatgagcg acatgatcgc ggcggagccc gcgctggcgg ggcgcgtggc
     9361 atcccggctg ggcgtggacg cggcggtcgc ctcc
//
LOCUS       DATACA010000341         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1488153, whole genome shotgun sequence.
ACCESSION   DATACA010000341 DATACA010000000
VERSION     DATACA010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488153"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VHK28_07020"
     CDS             complement(<1..369)
                     /locus_tag="VHK28_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2142010.1"
                     /translation="MPDGSVVVIGGNRGIGREIAKHYADAGADVVITCTDEERARAAC
                     EELGGSSRGIALDLTRPESMGEALADVGKVRYLVLVAIDRDHNTVREYDVARAMHLVT
                     LKLVGYTQVVHTLLDRMDEQS"
     gene            complement(362..1267)
                     /locus_tag="VHK28_07025"
     CDS             complement(362..1267)
                     /locus_tag="VHK28_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2142011.1"
                     /translation="MHLVSHVDWSFSDAPPEMAGSASGFARKMLVGRETGATHSELTV
                     CRLQPRGEVGRHMHSFEEASYVLAGQPVLELGERRLVLEAGDFALAPIGMAHGWSNPG
                     GEEARWLTISTPMRTTADEGRADTFAVEPLAVAGEPARPAFGHPSNRWVGHYAGTPPQ
                     AEALALPDPARGRRPAGMDTALLAYSGISVKMLIDRTFGAELLTMFTVDYEPNGAAQV
                     HDHPFEETYFFLDGEIEAVLDGKEYTIRAGDVVFAGVGSTHGFFNTGSGRVRWIETQA
                     PQPPARHAYRWVDVWKRFEKGAENA"
     gene            1378..1965
                     /locus_tag="VHK28_07030"
     CDS             1378..1965
                     /locus_tag="VHK28_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX2142012.1"
                     /translation="MTLVSLIEPVDADPSVRAPLDAGMEQYGQALHTWRALLHRPPIF
                     AAYLPYLRAVVGAGEVPQRTKELSALAVAIANRCRYSASHRYRAAQAAGVADAEVEAL
                     ARGDIDAFADPAERLAIEYARQLTLRPSEVPFGENPQAVDPQLLDALKAAFSEPQLVE
                     LTANIALWNALARFHRVMGFPLDMPPPPQAVEAAL"
     gene            2094..3302
                     /locus_tag="VHK28_07035"
     CDS             2094..3302
                     /locus_tag="VHK28_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2142013.1"
                     /translation="MNVDWEERVNTDRLRNYRFGRARDALNVSELGALLLFDFNNIRY
                     VTSTHIGEWARDKMTRYALLTRDGAPHLWDFGSAAKHHRLNCPWLPPENIRGGMIGLR
                     GAVAPDAGLFTRAAREIAEILRAEGVADMPVGVDILEPPMQSALEAEGIKVRDGQQTM
                     LDAREVKSLDEIVLLNTACAMVDGAYQLIAERLKPGVRESELVADVTKALFDMGSEHV
                     DNINAVSGERCSPHPHVFSDRLIRPGDQAFFDIIQTYVGYKTCYYRTFTVGSATDAQR
                     DAYKRAREWIDASIELMRPGVTTDQVASVWPKAQEFGFDSEMECFGLQFGHGVGLFLH
                     ERPIISRLNSLDNPIEIKEGMVIALETYCPAKDGYSAARIEEEVVVTAQGPQVITRFP
                     SQELFVANEY"
     gene            3302..4309
                     /locus_tag="VHK28_07040"
     CDS             3302..4309
                     /locus_tag="VHK28_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX2142014.1"
                     /translation="MATTAAHQTDIDRDVRIRMYRTMVECRTFEQRTHELFLAGLVRG
                     TTHLGIGQEAVAAGFAAAMRDDDYTFCTYPGHNHTLARGVPMTPILLELVGKQGALMG
                     GKGGSMHLTSVEHGVMGSYAIVGAHMPIAVGAAWSAQYRSSGQVAVCFFGDGTTNIGS
                     FHEALNLAAVWKAPVVFVCENNQYMEYTAIKEVTAVERPAADRASAYGLEPLVVDGND
                     VEAVFDMARRTIDRARDGLGPSLVEAITYRHGGHSRADPGKYRPDAEVKEWLARDPIP
                     AERQRLLKDGVEASVLDRIEAEAKEAVAAAEAVAREAPEPDLETAPTQVWADGGSAWR
                     S"
     gene            4297..>4444
                     /locus_tag="VHK28_07045"
     CDS             4297..>4444
                     /locus_tag="VHK28_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX2142015.1"
                     /translation="MAELTYRAAVAHGIAQEMERDPMVVMLGEDIGAAGGVFKTTEGL
                     LDKFG"
BASE COUNT          684 a         1598 c         1536 g          626 t
ORIGIN      
        1 gctctgctcg tccatgcggt cgagcagggt gtgcaccacc tgcgtgtagc cgaccagctt
       61 cagggtgacc aggtgcatgg cgcgcgcgac gtcgtactcg cggacggtgt tgtggtcgcg
      121 gtcgatggcc accagaacca ggtagcgcac cttgccgacg tcggccaggg cctcgcccat
      181 gctctccggc cgagtcaggt ccagggcgat gccgcgcgac gagccgccca gctcctcgca
      241 ggccgcgcgg gcgcgctcct cgtcggtgca ggtgatgacc acgtccgccc ccgcgtccgc
      301 gtagtgcttg gcgatctcgc gaccgatccc ccggttgccg ccgatgacca ccacgctgcc
      361 gtcaggcatt ctccgctccc ttctcgaatc gcttccagac gtccacccag cggtaggcgt
      421 ggcgggcggg cggctgcggc gcctgcgtct cgatccatcg cacgcggccg ctccccgtgt
      481 tgaagaagcc gtgcgtggat cccaccccgg cgaacaccac gtcgccggcg cggatggtgt
      541 actccttgcc gtccagcacc gcctcgatct ccccgtccag gaagaagtag gtctcctcga
      601 acgggtggtc gtgcacctgg gccgcgccgt tgggctcgta gtcgacggtg aacatggtca
      661 gcagctccgc cccgaaggtg cggtcgatca gcatcttgac gctgatgccg ctgtaggcca
      721 gcagcgcggt gtccatgccg gctggccgcc gtccgcgcgc cgggtctggc agcgccagcg
      781 cctcggcctg cggcggggtg ccggcgtagt ggcccaccca tcggttgctc ggatggccga
      841 acgccggtcg cgccggctcg cccgcaaccg ccagcggctc gaccgcgaag gtgtccgcgc
      901 ggccctcgtc agccgtcgtg cgcatgggcg tgctgatcgt gagccatcgg gcctcctcgc
      961 cacccgggtt ggaccagccg tgcgccatgc cgatgggtgc cagggcgaag tcgcccgcct
     1021 ccagcacaag ccgccgctcg ccaagctcca ggaccggctg cccggccagg acgtagctgg
     1081 cctcctcgaa ggagtgcatg tggcggccga cctcgccgcg gggctgcagc cggcagacgg
     1141 tgagctcgct gtgggtggcg ccggtctccc gcccgaccag catcttgcgg gcgaatccgg
     1201 aggcggagcc cgccatctcg ggcggggcat cgctgaacga ccagtccacg tgcgagacga
     1261 ggtgcatggg gcccgattgt agccctactg cacgccagaa tgaagcgggg ttgaacggca
     1321 gacgaggcgc ggggataatg gcggtgagtc gcatcggtcc acaccaggag cgtctgcgtg
     1381 accctcgtct ccctgatcga acccgtcgac gccgaccctt ccgtccgcgc cccgctggac
     1441 gcgggcatgg agcagtacgg gcaggcgctg cacacctggc gggcgctcct ccaccggccg
     1501 cccatcttcg ccgcctacct gccctacctc cgcgcggtgg tcggtgccgg cgaggtgccg
     1561 cagcgcacca aggagctgag cgcgctggcg gtggccatcg ccaaccgatg ccgctactcc
     1621 gccagccacc gctaccgtgc cgcccaggcc gccggcgtgg ccgatgccga ggtcgaggcc
     1681 ctggcccgcg gcgacatcga cgccttcgca gacccggccg agcggttggc gatcgagtac
     1741 gcgcggcagc tgaccctgcg cccgtccgag gtgccgttcg gcgagaaccc gcaggccgtg
     1801 gacccgcagc tgctggacgc cctgaaggca gcgttctccg agccgcagct ggtggagctg
     1861 acggccaaca tcgcgctgtg gaacgccctg gcccgcttcc accgggtgat gggcttcccg
     1921 ctggacatgc caccgccgcc gcaagccgtc gaagccgccc tctagctgct gaagacccgt
     1981 aggaggaacc gcacgtgacc gccgtcgaca ccgccgagac catcgagaac gtcgacaccg
     2041 tggcgcagcc cgcgtccgcg ccagcgatcc gtccgcccgc ctcgccgggc tccatgaacg
     2101 tcgactggga ggagcgggtc aacaccgacc gcctgcggaa ctaccggttc gggcgcgcgc
     2161 gggacgcgct gaacgtctcc gagctgggcg cactgctgct gttcgacttc aacaacatcc
     2221 gctacgtgac cagcacccac atcggcgagt gggcgcgcga caagatgacc cgctacgccc
     2281 tgctcacgcg ggacggcgcc ccccacctgt gggacttcgg ctcggccgcc aagcaccacc
     2341 gcctcaactg cccgtggctg ccgccggaga acatccgggg cggcatgatc ggcctgcgtg
     2401 gggcggtggc gccggacgcc ggcctcttca cccgggcggc gcgagagatc gccgagatcc
     2461 tccgggcgga gggcgtggcc gacatgccgg tgggcgtgga catcctggaa ccgcccatgc
     2521 agtccgccct ggaggcggag ggcatcaagg tccgcgacgg ccagcagacg atgctggacg
     2581 cccgcgaggt gaagtcactc gacgagatcg tgctgctcaa caccgcctgc gccatggtgg
     2641 acggcgccta ccagctgatc gccgagcggc tgaagccggg cgtgcgtgag agcgagctgg
     2701 tcgccgacgt gaccaaggcc ctgttcgaca tgggctccga gcacgtggac aacatcaacg
     2761 ccgtctccgg cgagcgctgc tcaccgcacc cgcacgtgtt cagcgaccgc ctcatccgtc
     2821 ccggcgacca ggccttcttc gacatcatcc agacctacgt cggctacaag acctgctact
     2881 accgcacctt cacggtgggc agcgccaccg acgcccagcg cgacgcctac aagcgggcgc
     2941 gcgagtggat cgacgccagc atcgagctca tgcggccggg ggtgaccacc gaccaggtcg
     3001 catcggtctg gccgaaggcg caggagttcg gcttcgacag cgagatggag tgcttcggcc
     3061 tgcagttcgg ccacggcgtc ggcctgttcc tccacgagcg accgatcatc agccgcctca
     3121 actcgctcga caatcccatc gagatcaagg agggcatggt catcgcgctg gagacctact
     3181 gcccggccaa ggacggctac tccgcggcgc gcatcgagga ggaggtggtg gtcaccgcac
     3241 agggcccgca ggtgatcacc cgcttcccgt cccaggagct cttcgtggcg aacgagtact
     3301 gatggccacc accgcagctc accagaccga tatcgaccgg gacgtgcgga tccgcatgta
     3361 ccgcaccatg gtcgagtgcc gcaccttcga gcagcggacc cacgagctgt tcctggccgg
     3421 gctggtgcgc ggcacgaccc acctgggcat cgggcaggag gctgtggcgg ccggcttcgc
     3481 ggccgccatg cgcgacgacg actacacctt ctgcacctac cccgggcaca accacaccct
     3541 cgcccgcggg gtacccatga cgccgatcct gctcgagctg gtcggcaagc agggcgccct
     3601 gatgggcggc aagggcggct ccatgcacct gaccagcgtg gagcacgggg tgatgggctc
     3661 gtatgcgatc gtgggcgcgc acatgccgat cgcggtgggc gctgcctggt cggcgcagta
     3721 ccgcagctcc gggcaggtgg cggtctgctt cttcggtgac gggacgacca acatcggttc
     3781 cttccacgag gccctcaacc tcgccgcggt ctggaaggcg ccggtggtct tcgtctgcga
     3841 gaacaaccag tacatggagt acaccgccat caaggaggtg accgccgtcg agcggccggc
     3901 cgccgatcgc gcctcggcct acgggctgga gccgctggtg gtggacggca acgacgtgga
     3961 ggcggtgttc gacatggcgc gccgcaccat cgaccgggcg cgggacggcc tgggcccctc
     4021 actggtggag gccatcacct accgccacgg cggccactcg cgggcggacc ccggcaagta
     4081 ccgcccggac gccgaagtca aggagtggct ggcgcgggat cccatccccg ccgagcgcca
     4141 gcgacttctg aaggacggcg tggaggcatc ggtcctggac aggatcgagg ccgaggccaa
     4201 ggaggccgtg gcagctgccg aggcggtcgc gcgcgaggcg ccagagccgg acctggagac
     4261 ggcgcccacc caagtctggg ctgacggagg ctcggcatgg cggagctgac ctaccgcgcc
     4321 gcggtggccc acggcatcgc ccaggagatg gagcgcgacc cgatggtggt catgctgggc
     4381 gaggacatcg gcgcggccgg cggcgtgttc aagaccaccg agggcctgct ggacaaattc
     4441 ggcc
//
LOCUS       DATACA010000342         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_927061, whole genome shotgun sequence.
ACCESSION   DATACA010000342 DATACA010000000
VERSION     DATACA010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927061"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VHK28_07050"
     CDS             <1..381
                     /locus_tag="VHK28_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX2142016.1"
                     /translation="AGRRRREVTSISGLLLCIAAAAGLALFYLSQSSHVAATGYRIAD
                     LQAQVAELRAEQQQLIYRIGEARSPAVIERRARGRLGLVPMPPEAIAFAPIATPVPSS
                     TPSASPGSVQPSGATTTSTQPTTH"
     gene            434..>1557
                     /locus_tag="VHK28_07055"
     CDS             434..>1557
                     /locus_tag="VHK28_07055"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2142017.1"
                     /translation="MLARTDSRARALFLLLVMTIVASGIGTRLVWWQVIDRGRLVAMA
                     DAQLAQTEEIPAERGEVTDANGVLLATSVELKSIFATPPAIDDVESTAALLAPVLGME
                     VGALRERLDRKDPWVWLRRRVDPAVSQRVRDLELRGVGMLAETQRVYPVAGTAPGTTL
                     AAQVLGFVNVEGVGQYGVEASYDDLLAGLPGSVTAQGDVTGRQIADSVRQLREPVDGS
                     DVRLTIDAGLQHILESAMWDNFRRNAAKGVTGVIMDVETGAIRALATFPAFDANEYGQ
                     TDPALFTNPAVSRQYEPGSVMKAFTIAAALDADAIDTNDTFEDDNNLRLAGVRIQNAD
                     RYTHRYGHGPITAGEVLQLSNNVGAAKIGLALGRDRLYDA"
BASE COUNT          239 a          570 c          532 g          216 t
ORIGIN      
        1 gccggccgac gccggcgcga ggtgaccagc atctccggcc tgttgctgtg catcgcagcg
       61 gcagccgggc tggcgctctt ctacctgagc cagtcgtccc acgtggccgc caccggctac
      121 cgcatcgccg acctgcaggc acaggtggcc gagctgcgcg ccgagcagca gcagctgatc
      181 taccgaatcg gcgaggctcg ctcgccggcc gtcatcgagc gtcgcgcgcg cgggcgcctg
      241 ggactggttc cgatgccgcc cgaggcgatc gccttcgcgc cgatcgccac gcccgtcccc
      301 agcagcacac cgtccgcaag ccccggatcc gtccagccca gcggagcgac gaccacatcg
      361 acccagccaa ccactcactg accattcacc ggcccagccc tcgggccggg tggagttcga
      421 acgcgagaga ccgatgctag ccaggaccga cagccgtgcc cgcgccctgt tcctgctgct
      481 ggtgatgacc atcgtcgcca gtggcatcgg cacgcgcctg gtgtggtggc aggtgatcga
      541 tcgcgggcgg ctggtggcca tggcggacgc ccagctggcc cagaccgaag agatccccgc
      601 cgagcgcggc gaggtgaccg atgccaacgg cgtactgctg gccacgtccg tggagctgaa
      661 atccatcttc gccacgccgc cggccatcga cgacgtggag agcacggcgg ccctgctggc
      721 gcctgtgctg ggcatggagg tcggcgcgct ccgcgaacgg ctggaccgca aggacccctg
      781 ggtctggctg cgccgccgcg tcgacccggc cgtctcgcag cgggtgcgcg acctcgagct
      841 gcggggcgtg ggcatgctgg ccgagacgca gcgcgtctat ccggtggccg gcaccgcgcc
      901 ggggaccacg ctggcggccc aggtgctcgg cttcgtgaac gtcgagggcg tgggtcagta
      961 cggcgtcgag gcctcgtacg acgacctgct ggccgggctg cccggctcgg tcaccgccca
     1021 gggggacgtc accggccgcc agatcgccga ctcggtgcgc cagcttcgcg agccggtcga
     1081 tggctcggac gtgcgcctca ccatcgacgc ggggctgcag cacatcctgg aatcggccat
     1141 gtgggacaac ttccgacgca acgcggcgaa gggtgtgacc ggtgtgatca tggacgtcga
     1201 aaccggcgcc atccgggccc tggccacctt cccggccttc gacgccaacg agtacggcca
     1261 gaccgatccg gcactgttca ccaacccggc cgtctcgcgc cagtacgagc ccgggtcggt
     1321 catgaaggcg ttcaccattg ccgccgcgct ggacgccgat gccatcgaca ccaacgacac
     1381 cttcgaggac gacaacaacc tgcgcctggc cggcgtccgc atccagaacg cggatcgcta
     1441 cacccatcgc tacggacatg ggcccatcac cgccggcgag gtgctgcagc tgtcgaacaa
     1501 cgtgggcgcg gccaagatcg ggctggccct cgggcgggat cggctgtatg acgcctt
//
LOCUS       DATACA010000343         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_204528, whole genome shotgun sequence.
ACCESSION   DATACA010000343 DATACA010000000
VERSION     DATACA010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204528"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VHK28_07060"
     CDS             <1..807
                     /locus_tag="VHK28_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2142018.1"
                     /translation="VAHMKELETQTGKGFGDPARPLLVSVRSGAAFSMPGMMDTVLNL
                     GLNPDTVQGLIAQTDNPRFGYDAWRRFVAMFGRIVLGIPGPEFDEPFDALKEERGAKL
                     DTDLSADDLREIADRYKAIVREKTGEDFPTDPYRQLELAIRAVFDSWFGKRAHDYREF
                     NKIAHDLGTAVNVVTMVFGNMGTDSGTGVAFTRDPNTGEKALYGEYLTNAQGEDVVAG
                     IRTPAKISQMRDELPEVYAQFEEIAERLERHYRDVQDLEFTIERGTLYML"
     gene            841..>2443
                     /locus_tag="VHK28_07065"
     CDS             841..>2443
                     /locus_tag="VHK28_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX2142019.1"
                     /translation="MKIAVDMANEGIVTREEALQRVDPAQIVQLLLPRFDEAAKEGAQ
                     DRFLAKGLNASPGAATGRAMFDPDRAVAAKEAGDPVILVRIETSPDDVHGMLAARGVL
                     TARGGATSHAAVVARSMGLPCVAGAEALKIDYAKRRMRAGSVTVDEGDMISIDGTTGE
                     IYAGELPTIEARFEDEHDLATLLGWADEVRRLQVWANADYPRDAERARAFGAQGIGLC
                     RTEHMFFEEERLPTVRRMILNAHTATAAKGRDEADRTDEDRAAVRVFDAALSELETAQ
                     TDDFAGLFRAMDGLPVVIRLIDPPLHEFLPSHDELLEKVTRLRTVLEMSGSGTTDAVA
                     DRVTPAIEKGLSRIFGEKDSARAMKLIRREGKASPAPDANGDRSGMQAELAEAEELLV
                     AVEAMREQNPMLGLRGIRLGLLIPDIVRMQTRAILAGAARVAAEGRKPLPEIMIPLVG
                     HVNELAETRGLLEAEVAKIVEASGQQVDYKFGTMIEVPRGALTAGEIAEHAEFFSFGT
                     NDLTQMTFGFSRDDAEGKFLLQYVDR"
BASE COUNT          421 a          836 c          854 g          332 t
ORIGIN      
        1 gtggcgcaca tgaaggagct cgaaacccag accggcaagg gattcggtga tccggccagg
       61 ccgctgctgg tcagcgtccg ctccggggcc gccttcagca tgccggggat gatggacacg
      121 gtcctcaacc ttggcctcaa ccccgacacc gtccagggcc tgatcgcaca gaccgataac
      181 ccgcgcttcg gctacgacgc ctggcgccgc ttcgtggcca tgttcggccg catcgtgctc
      241 ggcatccccg gtccggagtt cgacgagccg ttcgatgccc tcaaggagga gcgcggggcc
      301 aagctggaca cggacctgtc ggctgacgac cttcgggaga tcgcggaccg ctacaaggcg
      361 atcgtgcgcg agaagaccgg agaggacttc ccgaccgatc cctaccggca gctggagctc
      421 gccatccggg ccgtgttcga ctcctggttc ggcaagcgcg cccacgacta ccgggagttc
      481 aacaagatcg cgcacgatct gggcaccgcg gtgaacgtgg tgaccatggt cttcggcaac
      541 atgggaaccg actcggggac cggcgtggcc ttcacccggg accccaacac gggggaaaag
      601 gcgctctacg gcgagtacct gaccaacgcc cagggcgagg acgtggtggc cggcatccgc
      661 acgccggcca agatcagcca gatgcgcgat gagctgccgg aggtctacgc gcagttcgag
      721 gagatcgcgg agcgcctcga gcgccactat cgcgacgtcc aggacctcga gttcaccatc
      781 gagcgcggca cgctgtacat gctgtagact cgctcggcca agcgaaccgc accggctgcc
      841 gtgaagatcg ccgtcgacat ggccaacgag gggatcgtca cccgggagga ggcgctgcag
      901 cgggtcgacc ccgcgcagat cgtgcagctc ctgctgccgc gcttcgacga ggcggccaag
      961 gagggtgccc aggaccggtt cctggcaaag gggctcaatg cctcccccgg cgcggccact
     1021 ggcagggcca tgttcgatcc cgaccgggcg gtggccgcca aggaggccgg cgatccggtg
     1081 atcctggtgc gcatcgagac cagcccggac gacgtgcacg gcatgctggc cgcgcgcgga
     1141 gtgctcactg cccgcggggg cgcaacctcg cacgcggcgg tggtcgcccg ctccatgggc
     1201 ctgccctgcg tggccggagc ggaggcgctg aagatcgact acgccaagcg ccgcatgcgc
     1261 gcgggcagcg tgaccgtcga cgagggcgac atgatcagca tcgacggcac gaccggcgag
     1321 atctacgccg gcgagctgcc gaccatcgag gcccgcttcg aggacgaaca cgacctggcc
     1381 accctgctgg gctgggccga tgaggtccgc cgcctccagg tgtgggccaa cgcggactac
     1441 ccgcgcgacg ccgagcgggc tcgcgccttc ggcgcgcagg gcatcggtct atgccgcacc
     1501 gagcacatgt tcttcgagga ggagcggctg ccgaccgtac gccgcatgat cctcaacgcc
     1561 catacggcca ccgcggccaa gggcagggac gaagcagacc ggaccgatga ggaccgcgcc
     1621 gcggtgcgcg tcttcgacgc cgccctgtcc gagctggaga ccgcccaaac cgatgacttc
     1681 gccggcctgt tccgggccat ggacggcctg ccggtggtga tccgcctcat cgacccgccg
     1741 ctgcacgagt tcctgccctc gcatgacgag ctgctggaga aggtgacccg cctgcggacc
     1801 gtcctcgaga tgtccggctc cgggacgacc gatgccgtgg cggaccgtgt gacacctgcc
     1861 atcgagaagg gcctgtcgcg catcttcggc gagaaggact cggcgcgcgc catgaagctc
     1921 attcgccgcg agggcaaggc cagcccggcc ccggacgcca acggcgaccg ctccgggatg
     1981 caggccgagc tggccgaggc ggaggagctg ctggtggcgg tggaggccat gcgtgagcag
     2041 aacccgatgc tcgggctgcg cggcatccgg ctgggactcc tgatcccgga catcgtgcgc
     2101 atgcagacca gggccatcct ggccggggcg gcgcgcgtcg ccgccgaggg ccgcaagccg
     2161 ctgcccgaga tcatgatacc gctggtcggg catgtgaacg agctggccga gaccaggggt
     2221 ctgctggagg ccgaagtggc caagatcgtc gaggcctccg gccagcaggt ggactacaaa
     2281 ttcggcacga tgatcgaggt gccgcgcggc gcgctgaccg ccggcgagat cgccgagcac
     2341 gccgagttct tcagcttcgg caccaatgac ctgacgcaga tgaccttcgg attctcacgg
     2401 gacgacgccg agggcaagtt cctgctgcag tacgtcgacc gca
//
LOCUS       DATACA010000344         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_766071, whole genome shotgun sequence.
ACCESSION   DATACA010000344 DATACA010000000
VERSION     DATACA010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766071"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..464)
                     /locus_tag="VHK28_07070"
                     /pseudo
     CDS             complement(261..464)
                     /locus_tag="VHK28_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(605..1939)
                     /locus_tag="VHK28_07075"
     CDS             complement(605..1939)
                     /locus_tag="VHK28_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2142020.1"
                     /translation="MSGASIETTLELWASSLRDVKARIRPLFTQDRVAASASQFLDGL
                     LGPERRKTGWMRAEAAGDPGPWRQQAILGRGRWEADALCDIVRDYALETLADADAVLV
                     IDETGFLKQGKASCGVGRQYTGSAGKITNCQIGVFAAYASWHGHAFVDRALYLPRSWT
                     DDAARLEAAHVPAAVGFATKPRLARQMIERAIAAEVPFAWVAADSVYGVGEIEMALRR
                     AGKGYVLGVNATQPFNSWIGKPMVAGTAEEIARDLEPSAWRRLSAGEGTKGPRLSDWA
                     YLELADLDADEYRDGATGVWTRGLLIRRNLSDGECAYFTTWCPTGTTIETLTTVEGQR
                     WAIEDAFETAKNELGLDHNETRSWHGWHRHVSLVMLAFAMLATIRHKANQQPPPKTLI
                     RLTRTRRTSSAGRSKRSAASQSASRSGGSSQPTSSLGHSGDARTKPPHNART"
BASE COUNT          346 a          762 c          716 g          363 t
ORIGIN      
        1 gacgacggtg cacgcatcac cgaagaactg gcagagccgg ttaatctgac attccccgga
       61 tggctgccga tttcgtcggc ttggagcaaa gggtagccat cgccagcccc taccggcaac
      121 aacctcatcg gtcggggtgg caccgggacc gctgccgcgg tgcgatgacc tccaaggcgg
      181 cggggattca cccccgaaat ccgcctctcg agcgcacctc tcccaccgac atcctggcac
      241 gccggtcgcg gcgccgatcc tcagcatcgg gtccccggcg gtgggcgcaa cgctgcgatg
      301 gcggccagga tctcgcggaa catgcctcga ggcaccatca cctcggccat ctggaagatc
      361 accgagcggc cgtgccggac gatcctggcg ccgatctcca ccaaccgctc gcgcagcgtg
      421 gtcatggacc acggcgccgc ctcggccggc agcgccaggg tgcgtcgctc cgtgtcgcgc
      481 tcctgaagga gttgttcgac cttctcccgg tggcagcttc cgcgtgagcc gggccggtcg
      541 cagctccaaa agctgcagcg attggggtaa gggataagct agcattacag ctgcattttg
      601 cgcttcaggt gcgagcgttg tgcggcggct tggtgcgcgc gtcgccagag tgaccaagcg
      661 atgacgtggg ctggctggat ccgccgctgc gcgaggcgga ttgcgatgcg gcggatctct
      721 tggaccgacc agcggatgag gtccggcgcg tccgcgtcag cctgatcagg gtttttgggg
      781 gcggttgctg gttggctttg tggcgaatgg tcgccagcat ggcgaaggcc aacatcacca
      841 gcgagacatg gcgatgccag ccatgccacg agcgggtctc attgtggtcg aggccgagct
      901 cgttcttcgc tgtctcgaag gcgtcctcga tggcccagcg ctgcccttcg actgtggtca
      961 gcgtctcgat cgtggtcccg gtggggcacc aggtggtgaa gtaggcacat tcaccatcgc
     1021 tgaggttgcg ccggatcagc aagccacgcg tccagacgcc cgtcgcgcca tcccggtact
     1081 cgtccgcgtc caggtcggcc agttcgaggt aggcccagtc ggaaagccgc ggccctttcg
     1141 tgccctcgcc cgcggacagg cgacgccagg cagagggctc gagatccctg gcaatctcct
     1201 cggcagttcc agccaccatc ggcttgccga tccaggagtt gaagggctgc gtggcgttga
     1261 ccccgagcac gtagcccttg cccgcccggc gcagcgccat ctcgatctcg cccaccccgt
     1321 aaacgctgtc cgccgccacc caggcgaacg gcacctcggc cgcgatggcg cgctcgatca
     1381 tttgcctggc cagccgcggc ttggtggcga agcccaccgc ggctgggaca tgcgccgcct
     1441 ccagccgggc cgcgtcgtcg gtccagctcc tgggcaggta gagggcgcgg tcgacgaagg
     1501 cgtgcccgtg ccatgaggca taggccgcga agaccccgat ctggcagttg gtgatcttgc
     1561 cagccgagcc ggtgtactgc cgcccgacgc cgcaggacgc cttgccctgt ttgaggaagc
     1621 cggtctcgtc gatgaccagc acagcatccg cgtcggccag cgtctccagc gcgtagtcgc
     1681 gcacgatgtc gcacagcgca tccgcctccc agcggccgcg gccgaggatg gcctgctgcc
     1741 gccacggccc gggatcgccc gccgcctcgg cccgcatcca gcccgtcttg cgccgctcag
     1801 gcccgagcag cccatcaaga aactgactgg ccgaggccgc cacccggtcc tgggtgaaca
     1861 agggacggat ccgcgccttg acgtcgcgca gcgacgacgc ccacagctcc agcgtcgtct
     1921 cgattgatgc accactcacc ggctcacctc cggcaaagcg gagcaacgct tcaggtcagg
     1981 aaaagtgcag ctgtaatgct tccgtaatgg gccttcgggt gtcaagcccc tcgagtgcgg
     2041 ttctacaacg cggtactcca tttccttggt cgtatctgga cgtgcgttac tcttccgctg
     2101 acgcgtgggc agaccgccgc gtcgacggca ccaatgccgc cctctcggcg acgagccgcg
     2161 gtcaagcccc gcagccgcac agcggtg
//
LOCUS       DATACA010000345         4582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1810553, whole genome shotgun sequence.
ACCESSION   DATACA010000345 DATACA010000000
VERSION     DATACA010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810553"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VHK28_07080"
     CDS             complement(<1..1175)
                     /locus_tag="VHK28_07080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142021.1"
                     /translation="MSSGNTRFPGLRRGFEALATRNFRLYWTGQGISRIGTWMQQISL
                     PWLVLELGGSPIQLGIVAALEFVPSLLLAPFGGVLVDRIDKRRALLVTQSLATVQVVV
                     LLAVTVTGLVSIPLVMALALVLGLINALDMPVRQSLAADLVPRELLPNAIALNSMAFN
                     GARVIGPALGGVIIAIGAGLFGSNLDGIAFNFGVNVLSYSAVLISLARMDRSQIRRTP
                     RDAEPVPMLSSLREGVVFAGRSPMILWGLVLLGLVATFGLNFRILLPIFAQQELGLDA
                     DGYGLLYAATGLGSLIGSLTLAYMRQRRAIMLMLGGGLAFGALELLLAGTRSLPTAAP
                     LALGAGLSSMLMINTVNATVQANVTDAVRGRVMALYVTVFAGTSPFGGIFAGAVAER"
     gene            complement(1275..2573)
                     /locus_tag="VHK28_07085"
     CDS             complement(1275..2573)
                     /locus_tag="VHK28_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX2142022.1"
                     /translation="MQEVFADDWQAFVDGGLYRSLVDAGLLVDHEEAPVQDAALPGAV
                     AVIRPRELPLISYPYEWCFTQLREAALLTLELQRRALVAGMRLKDASAYNIQFDRGRP
                     ILIDTLSFERAEPGEPWPAYRQFCEHFLAPLALVAYRDPRCTLMLRDFIDGIPVDLAS
                     TLLPVRTRLRPGLLAHIHVHAGAQRRAEAAEPTAEHRPARRMSKLGQEALLDNLRRAI
                     EGLRWQPHGHWVEYGTRTSYSDRGAESKREIVVRMLGQTDAQTVWDLGANVGTYSTIA
                     AGSGRQVLAFDQDANAVEHHWRQLSADARASVLPLVLDLANPSPGLGWALQERRSIID
                     RGPGDMILALALVHHLAIGNNVPLQRVAALFASLGRRAIVEFVPKEDPMTRHLLSSRR
                     DVFPGYTVDGFRAAFGQHFRLVEEAPIEDSARTLFLLERT"
     gene            complement(2663..2848)
                     /locus_tag="VHK28_07090"
     CDS             complement(2663..2848)
                     /locus_tag="VHK28_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142023.1"
                     /translation="MNLFAYIDPGSGSLLLQALLAGLLAVPFFFRRTIGGFVSRIRGE
                     KPESADPPAPPRDGPER"
     gene            2935..4467
                     /locus_tag="VHK28_07095"
     CDS             2935..4467
                     /locus_tag="VHK28_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142024.1"
                     /translation="MHPLLLAAGPVLFLFAQNAEQQVTLGPLWRPLIVVLLGAGVTLV
                     ACIAALRDVQRGALLAGLLVALFMSFGHVWNATGDLLLEPIWLAPFYVGAALAGGFLA
                     WRGGRWVAGANRFVSVAAAVFVVINLVRVGDFALGSIGPTQETVERAPILVNEEAPRP
                     DIYYLILDRYAGPDVLREVYDMDPEPFLGELEERGFAVARDSWANYFKTALSVASSLE
                     MSYLDPERYDDSDPDSFDRVHASLRQRLAVPATLKALGYEYVHIGNTWEPNATNVDAD
                     IVLRYEEGSEFETAVMATTPLMLAGPAVLPDDDDAETLPFPELNRKHILFGFDRLEEA
                     ASRPGPTFVFAHILVPHPPYVFDTDGSMPTEAERRERRETEEYRRQVAWTNQRTLAAL
                     DRLLSAPGEGEPVIVLQSDEGPWPPRFSADQTDFQWLEATDAEIHWKYSILNALRLPG
                     VDAREAGFDNSISPVNEFRVVFNALFGSNLPLLPDRYNLSPDYDHMWSLVEYERPDAP
                     VF"
BASE COUNT          746 a         1535 c         1630 g          671 t
ORIGIN      
        1 cagcgctccg ccaccgcgcc ggcgaagatg cccccgaagg gcgaggtacc ggcgaagacc
       61 gtgacgtaga gagccatcac tcgaccgcgg accgcatcgg tcacgttggc ctgcaccgtc
      121 gcgttcacgg tgttgatcat cagcatcgac gagagtccgg cgcccagcgc cagaggcgcg
      181 gcggtcggca ggctgcgagt tccggccagc aggagttcca gcgcgccgaa ggcgagcccg
      241 ccgccgagca tgagcatgat cgcccgccgt tgccgcatgt aggccagcgt cagcgagccg
      301 atcagcgagc ccaggccggt ggccgcgtac agcagcccgt agccgtccgc gtccagcccg
      361 agttcctgct gcgcgaagat cggcagcaga atgcggaagt tcagcccgaa ggtggccacc
      421 agccccagga gcacgaggcc ccacaggatc atcggcgacc ggccggcaaa gacgacacct
      481 tcccgaaggc tgctgagcat ggggaccggc tcggcgtcgc ggggcgttct ccggatctgg
      541 ctgcggtcca tccgagccag gctgatgagc acggcgctgt agctgagcac gttgacgccg
      601 aagttgaagg cgatgccgtc gaggttactg ccgaacaagc cggcgccgat ggcgatgatg
      661 acccctccca gcgcaggacc gatgacccgc gctccgttga aggccatcga gttgagggcg
      721 atcgcattgg gaagcagctc gcgcggcacg aggtctgcgg cgagcgactg gcggaccggc
      781 atgtccaggg cgttgatcag gccgagcacc agtgcgagcg ccatcaccag cgggatgctg
      841 acgagcccag tcacggtgac cgccagcagc acgacgacct gcacggtggc cagcgactgg
      901 gtgacgagca gggctcttcg cttgtcgatg cgatccacca gcacgccgcc gaacggcgcc
      961 agcagcaggg aaggcacgaa ctccagggcg gcgacgatgc cgagctggat gggggaaccg
     1021 ccgagctcca gcacgagcca ggggaggctg atctgctgca tccaggttcc gatgcggctg
     1081 atcccttgcc cggtccaata gagccggaaa ttccgcgtgg cgagcgcctc aaatcctcgt
     1141 cgcagcccgg ggaagcgcgt attgccggag gacatggtta gccatgctaa caatatggcc
     1201 aagacgcgtg aatcgaggtt cgcatctcgc tagtcgggga tcgcccggcc cggcgagcag
     1261 cgctcagatc tcggtcaggt gcgctccagc aggaacaggg tgcgggcgct gtcctcgatc
     1321 ggcgcctcct cgacaagccg gaagtgctgc ccgaacgccg cgcggaagcc atccacggtg
     1381 tacccgggga acacgtcgcg gcgggaggag agcagatgcc gtgtcatcgg atcctccttt
     1441 ggcacgaact cgacgattgc gcgtcggccg agtgaagcga agagcgccgc cacccgctgg
     1501 agcggtacgt tgttgccgat ggcgaggtgg tggaccagcg ccagcgccag gatcatgtcg
     1561 cccgggccgc ggtcgatgat cgagcggcgc tcctgtagcg cccagcccag gccggggctg
     1621 gggttcgcaa ggtccaggac cagcggcagg accgatgctc gcgcgtcggc ggacagctgc
     1681 cgccagtggt gctcgaccgc gttggcgtcc tggtcgaagg cgagcacctg ccgcccggac
     1741 cccgcggcga tggtcgagta ggtgcccacg ttggcgccca ggtcccagac ggtctgcgca
     1801 tcggtctggc ccagcatgcg caccacgatc tcgcgcttgg actccgcccc acgatccgag
     1861 tacgaggttc gagttccgta ctccacccag tggccgtgcg gctgccagcg cagtccctcg
     1921 atggcacgcc gcaggttgtc cagcagcgcc tcctgcccga gcttgctcat gcggcgcgcc
     1981 gggcggtgct cggccgtcgg ctcggcagcc tccgctcgcc gctgggcgcc ggcgtggacg
     2041 tggatgtgcg ccagcagccc ggggcggagg cgcgtgcgca ccggcagcag ggtggaggcc
     2101 aggtccaccg ggatgccgtc gatgaagtcg cgcagcatca gcgtgcagcg cgggtcgcgg
     2161 taggcgacca gggccagcgg cgccaggaag tgctcgcaga actggcggta ggccggccac
     2221 ggctcgccgg gctcggcgcg ctcgaaggag agcgtgtcga tcaggatcgg acggccgcgg
     2281 tcgaactgga tgttgtaggc cgaagcgtcc ttgaggcgca tgccggcaac cagcgcgcgt
     2341 cgctgcagct ccagcgtcag cagcgccgcc tcgcggagct gggtgaagca ccactcgtac
     2401 gggtacgaga tcagcggcag ctcacgaggg cgaatgacgg cgactgcacc cggcagggcc
     2461 gcatcctgga cgggtgcctc ctcgtgatcg accagcaacc cggcgtccac gagggaccga
     2521 tacagccctc cgtcgacgaa cgcctgccag tcgtcggcga agacctcctg cacctggcgg
     2581 tgcaggatgc cgtcatgccg atagacgtag ccgctcggat cccggaacga tgccgggtcg
     2641 accgatggcg gcgacggagc tcctatcgct caggcccgtc gcgtggcggt gcggggggat
     2701 cggccgattc gggcttttca ccccggatgc ggctcacgaa accgccgatc gtgcggcgga
     2761 agaagaacgg gacggccagc aaccccgcca ggagcgcctg gagcaggagg ctgcccgacc
     2821 cgggatcgat gtaagcgaat aggttcattc ggggccgttc ccctttcacg tagtgttggg
     2881 cggatgttac cccgcttaac cagcaagcag cgcgccgggc tcaggagcgt gcccatgcac
     2941 ccactgctgc tggccgccgg gccggtgctg ttcctgttcg cgcagaacgc ggagcagcag
     3001 gtcacgctcg gaccgttgtg gcggccgctg atcgtcgtgc tgctgggggc gggagtgacg
     3061 ctggtggcct gcatcgccgc cctgcgcgac gtgcagcgcg gcgcgctgct ggcgggcctg
     3121 ctggtggcgc tgttcatgtc cttcggccac gtgtggaacg caaccggcga cctgctgctg
     3181 gagcccatct ggctggctcc cttctacgtg ggcgccgcgc ttgccggcgg cttcctggcc
     3241 tggcgcggcg gccgctgggt ggctggcgcc aatcggttcg tcagcgtggc ggcggcggtg
     3301 ttcgtggtaa tcaacctggt gcgcgtcggg gatttcgcgc tgggaagcat cggtccaacc
     3361 caggaaaccg tggaacgagc acccatcctg gtcaacgagg aggcccctcg accggacatc
     3421 tactacctca tcctggaccg atacgccggg cctgacgtgc tccgggaggt ctacgacatg
     3481 gaccccgagc ccttcctggg cgagctggag gagcgcggct tcgcggtggc gcgcgactcg
     3541 tgggccaact acttcaagac ggcgctgtcg gtggccagct ccctggagat gtcgtacctg
     3601 gacccggagc gctacgacga ctccgacccg gactccttcg accgggtgca cgcctcgctg
     3661 cggcagcggc tggcggtgcc cgccaccctc aaggcgctgg ggtacgagta cgtgcacatc
     3721 gggaacacct gggagccgaa cgccaccaac gtggacgccg acatcgtgct gcgctacgag
     3781 gagggctcgg aattcgagac ggcggtcatg gccaccacgc cgctgatgct ggccggccct
     3841 gccgtcctgc ccgatgacga cgacgccgag acgctcccgt tcccggagct gaaccgcaag
     3901 cacatcctgt tcggctttga tcgcctggag gaggctgcca gccggcccgg gcccaccttc
     3961 gtcttcgccc acatcctggt gccgcacccg ccctacgtgt tcgacacaga tggctcaatg
     4021 ccgaccgagg ccgagcgacg ggagcggcgc gagaccgagg agtaccgccg gcaggtggcg
     4081 tggaccaacc agcggacgct ggcggcgctc gaccgcctgc tcagcgcacc gggggagggg
     4141 gagccggtga tcgtgctgca gtcggacgag ggcccgtggc ctccgcgctt cagcgccgac
     4201 cagaccgact tccagtggct ggaggctacg gacgcggaga tccactggaa atactcgatc
     4261 ctgaacgcgc tgcggctgcc gggcgtggat gcgcgcgagg ccgggttcga caacagcatc
     4321 tccccggtga acgagttccg ggtcgtgttc aacgccctgt tcggctcgaa cctgccgctg
     4381 ctcccggacc gctacaacct ctcgccggac tacgaccaca tgtggtccct ggtcgagtac
     4441 gagcggccag atgcgccggt cttctagccc atcccgtcca tggacgccat gacggcccgc
     4501 tctgccaggg cgatgggcag cgtgccggcg gaggcgatga cgtcgtggaa ggcgcgcagg
     4561 gcgtcgacgt cggttgcatc gg
//
LOCUS       DATACA010000346         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_204770, whole genome shotgun sequence.
ACCESSION   DATACA010000346 DATACA010000000
VERSION     DATACA010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204770"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="rplE"
                     /locus_tag="VHK28_07100"
     CDS             <1..233
                     /gene="rplE"
                     /locus_tag="VHK28_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136285.1"
                     /note="part of 50S and 5S/L5/L18/L25 subcomplex; contacts
                     5S rRNA and P site tRNA; forms a bridge to the 30S subunit
                     in the ribosome by binding to S13; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2142025.1"
                     /translation="ALALPRIRDFRGIPTRSFDGRGNFSLGLREQLVYPEIDYDKIDR
                     LRGLEISIVTTAKTDEEGRRLLQLLGMPFQAN"
     gene            246..431
                     /locus_tag="VHK28_07105"
     CDS             246..431
                     /locus_tag="VHK28_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX2142026.1"
                     /translation="MAKKSMIAKARRGARFPVRQHNRCSICGRPRGYMRRFALCRICF
                     RERALLGQLPGVTKSSW"
     gene            526..924
                     /gene="rpsH"
                     /locus_tag="VHK28_07110"
     CDS             526..924
                     /gene="rpsH"
                     /locus_tag="VHK28_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2142027.1"
                     /translation="MTTTDPITDLLTRIRNASAARHTEVTLPSSRVKREIARILAEEG
                     FVGGVSTSQDGVQEMLTVQLKYVEGRTPVVTGLKRISKPGLRVYARKTEIPRVLGGLG
                     MAILSTSQGIMTGSQARKLNLGGEVLCYVW"
     gene            937..1485
                     /gene="rplF"
                     /locus_tag="VHK28_07115"
     CDS             937..1485
                     /gene="rplF"
                     /locus_tag="VHK28_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2142028.1"
                     /translation="MSRIGRMPIPLPDNVEVAQEGRRLRVKGPLGTLEREIHAEMRVE
                     QADGELRVVRPSDQPRHRALHGLTRTLVANMVTGVTTGFSRGLEISGVGYRAQLQGSK
                     LVLALGYSHPVEVDAPEGIEFRVETPTRLAVFGADKELVGQTAAFIRSRRKPEPYKGK
                     GIRYAGEQILRKAGKAGKVGGG"
     gene            1485..1844
                     /gene="rplR"
                     /locus_tag="VHK28_07120"
     CDS             1485..1844
                     /gene="rplR"
                     /locus_tag="VHK28_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014918232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2142029.1"
                     /translation="MIKKKSRDELRRKRHERIRLRLSGTTERPRLSVFRSTKYIYAQV
                     IDDTTGTTLAAASSREAELRDGQGKVDVAKAVGKALAERARAKGVSAVVLDRGGYQYH
                     GRVRSLAEGAREGGLDL"
     gene            1861..2358
                     /gene="rpsE"
                     /locus_tag="VHK28_07125"
                     /pseudo
     CDS             1861..2358
                     /gene="rpsE"
                     /locus_tag="VHK28_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            2477..2677
                     /gene="rpmD"
                     /locus_tag="VHK28_07130"
     CDS             2477..2677
                     /gene="rpmD"
                     /locus_tag="VHK28_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2142030.1"
                     /translation="MLRVTWVRSTIGHKAAARGTIRALGLHRLNQTIEIADSPVNRGM
                     LRRVAFLVNVQEPASGEEGTSQ"
     gene            2674..>3227
                     /gene="rplO"
                     /locus_tag="VHK28_07135"
     CDS             2674..>3227
                     /gene="rplO"
                     /locus_tag="VHK28_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX2142031.1"
                     /translation="MKLHDLQPAKGSHKPPRRVGRGHGSGRGKTAGRGTKGQKSRSGS
                     GIPAWFEGGQTPLHVRTPKLHGFKNRFRVAYSGVNLARLTDVQPGTLITPDVLKHDGL
                     VRDSKTKPLPIKILGGGDAPRGVTIHAHAFTRSALVKLEAAGSTAQRISWPEGLPVED
                     MEGDPEGDVQAAAESKGERKARAKA"
BASE COUNT          558 a         1114 c         1148 g          407 t
ORIGIN      
        1 tggcgctcgc gctgccccgc atccgggact tccgcggcat ccccacccgc tcgttcgacg
       61 gtcgcggcaa cttcagcctc ggcctgcgcg agcagctggt gtatccggag atcgactacg
      121 acaagatcga ccggctgcgc gggctggaga tcagcatcgt caccaccgcc aagaccgacg
      181 aggaggggcg ccgcctgctc cagctcctcg gcatgccgtt tcaggccaat taggtaggag
      241 agcccgtggc caagaagtca atgatcgcca aggcacgtcg cggcgccagg tttcccgttc
      301 ggcagcacaa ccgctgctcg atctgcgggc ggccgcgcgg ctacatgcgc cgcttcgcgt
      361 tgtgccgcat ctgcttccgg gagcgcgccc tgctgggcca actgccggga gtgaccaagt
      421 ccagctggta agaaccaccc ggccgcacag gcggccccat cagcggcagg cgccaaccac
      481 gctgcgcgtg gcgcgaacca ccgccgagga gacgaatcgc aaaccatgac caccactgat
      541 ccgatcaccg acctgctgac gcgcatacgc aacgcgagcg cggctcggca caccgaggtg
      601 acgctgccct cgtccagggt caagcgcgag atcgcgcgca tcctagcgga ggagggcttc
      661 gtcggcggcg tgtcgaccag ccaggacggc gtgcaggaga tgctcaccgt gcagctgaag
      721 tacgttgagg gccgcacgcc ggtcgtcacc ggcctgaagc gcatcagcaa gccggggctg
      781 cgggtgtacg cgcgcaagac cgagatcccg cgcgtccttg gcgggctcgg catggccatc
      841 ctttccacgt cgcaggggat catgaccggg tcgcaggccc gcaagctgaa cctcggcggc
      901 gaggtcctct gctacgtctg gtaagggagc gaactgatgt cacgaatcgg tcgaatgccg
      961 atcccgctcc ccgacaacgt ggaggtcgcc caggaagggc gccggctgcg ggtcaagggc
     1021 cccctgggca ccctcgagcg ggagatccac gccgagatgc gcgtggagca ggctgacggt
     1081 gagctgcgcg tggtgcgccc cagcgaccag ccgcgccatc gggcgctgca cggcctgacg
     1141 cgcaccctgg tggcaaacat ggtgaccggg gtgaccaccg gcttctcgcg agggctcgag
     1201 atcagcggag tcggctaccg cgcccagctg cagggcagca agctggtcct cgccctcggc
     1261 tacagccacc cggtcgaggt ggacgcgccg gagggaatcg agttccgggt ggagacgccg
     1321 acgcggctgg cggtcttcgg cgccgacaag gagctggtgg gccagacggc agcattcatc
     1381 cgttcgcgac gcaagcccga gccgtacaag ggcaagggca ttcgctacgc gggcgagcag
     1441 atcctgcgca aggcgggcaa ggccgggaag gtcggaggcg gatagtgatc aagaagaaga
     1501 gccgcgacga gctgcgacgc aagcggcacg agcgcatccg gctgcgcctg agcgggacga
     1561 cggagaggcc gcgcctgtcg gtcttccgca gcacgaagta catctacgcc caggtcatcg
     1621 acgacaccac cggcaccacc ctggccgctg catccagccg agaggcggag ctgcgcgacg
     1681 ggcagggcaa ggtggacgtc gcgaaggccg tgggcaaggc gctcgcggag cgcgccaggg
     1741 ccaagggcgt gagcgccgtg gtcctggacc gtggcggcta ccagtaccac gggcgggttc
     1801 gctcgctcgc cgagggcgcc cgcgagggcg gcctcgacct gtagagcgga ggcacagcac
     1861 atggcaagaa tcgacccctc caagctcaac ctggaagaga aggtcgtcca gatcaaccgc
     1921 gtggcaaagg tcgtcaaggg cggccggcgc ttcagcttca gtgcggtggt ggtcgtcggc
     1981 gatcgcaacg gcgtggtggg cgccggcctc ggcaaggccg gcgaggtgcc cgaggccatc
     2041 cgcaagggcg ttgaggacgc caagaagcgc ctcatccgcg tcccgctggt gggcagcacc
     2101 atcccgcacg acgtcttcca ccgttacggc gcctctcgca tcatgctgaa gccggcctcg
     2161 gccggcaccg gcgtgatcgc gggcggctcg gtgcgcgccg tcgtggaggc cgcgggcatc
     2221 cgtgacctgc tgagcaagac cctgggcagc accaacccgg tgaacgtggt gcgcggcacc
     2281 gtcgaggccc tggcctccct gcggtccgcc gatgcgatcg ccggccgccg cggaaagacc
     2341 gccgaggagc tgctcggcaa gcgcggcgcc gagttcgcgc ggcgtccgca cgagtacgtg
     2401 gagcggcccg cgtcgccgtt cccgaccgga ggcggacgtg gccgctaagc gccgctccac
     2461 cagtcaggcc gggccaatgc tccgcgtgac ctgggtgcgc tcgaccatcg gccacaaggc
     2521 cgcggcgcgt ggcaccatcc gggcgctcgg gctgcaccgg ctgaaccaga ccatcgagat
     2581 cgccgattcg ccggtcaacc gtggcatgct gcgccgcgtt gcgttcctgg tgaacgtcca
     2641 ggagccggca tcgggcgagg aaggaacctc ccagtgaagc tgcacgatct ccagcccgca
     2701 aagggctcgc acaagccgcc gcgccgcgtc gggcgcgggc acggctcggg tcgtggcaag
     2761 accgccggcc gcggcaccaa gggacagaag tcgcgctccg gcagtggcat tcccgcctgg
     2821 ttcgagggcg gccagacgcc gctgcacgtc cgcaccccga agctgcacgg cttcaagaac
     2881 cggttcaggg tcgcgtactc gggcgtcaac ctcgcccgcc tgaccgatgt gcagcccgga
     2941 acactcatca cccccgacgt gctcaagcac gacggcctgg tgcgcgacag caagaccaag
     3001 ccattgccca tcaagatcct gggcggcgga gatgccccca ggggcgtgac gatccacgcc
     3061 cacgccttca cccgctccgc gctggtcaag ctggaggcgg cggggagcac cgcgcagcgc
     3121 atcagctggc cggagggcct gccggtcgaa gacatggagg gcgacccgga gggcgacgtg
     3181 caagccgccg cggagtcgaa gggcgagcgc aaggcacggg ccaaggc
//
LOCUS       DATACA010000347         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1248398, whole genome shotgun sequence.
ACCESSION   DATACA010000347 DATACA010000000
VERSION     DATACA010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248398"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1168)
                     /locus_tag="VHK28_07140"
     CDS             complement(2..1168)
                     /locus_tag="VHK28_07140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2142032.1"
                     /translation="MHRLRQPESLLVGLTLLVKLGVLGLGAIAHLVIVRGDISGPLDV
                     LRPWNDWDAPHYLDLIVFGYRAVDPGNLVGPGGYRSVFPGDLPLYIVFYPLYPWLATA
                     VNAVVNQPLVSAFVVSSVASLFVAPLLYRLVRHEEGPGIALRAAWLCLIFPTAFFLHI
                     GYTEALFLALVLGSFLAARTDRWWLAGLLGALAALTRVNGLVLLLALPAEAATQWFEQ
                     PAGERRFRLRWLAVGLVLIGPAAYLGLNQAVYGDAFHFLAVQESHWFKSLEPPWVAIA
                     TAFRWFGSRNVDTMVMYGGMELAFTALGLAATVYAALRFRPSWFAWMAGNWLMFTSTG
                     FLVSVPRYSLTLFPLFVAMALVSRRAGVLVAISTVSLGLFVYFALRFSSGAWAF"
     gene            1297..1373
                     /locus_tag="VHK28_07145"
     tRNA            1297..1373
                     /locus_tag="VHK28_07145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1331..1333,aa:Pro,seq:cgg)
     gene            complement(1382..1765)
                     /locus_tag="VHK28_07150"
     CDS             complement(1382..1765)
                     /locus_tag="VHK28_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2142033.1"
                     /translation="MPRDPNIPDAFDVYTLVLLRRPPDAPEMSEAELHALQALHLAYR
                     ARLRNEGVIVANGPFGEQSDVTMRGLSIFACDIDEARRLSDADPSVQAGRLAYDVMEW
                     WVAANSMAFPEATRPVGERRVMADD"
     gene            1884..2390
                     /locus_tag="VHK28_07155"
     CDS             1884..2390
                     /locus_tag="VHK28_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142034.1"
                     /translation="MSVHRVGDLEVEQDLAFQRREWIAERIGWTAMLVLAVAGVLGLF
                     GTGPLAAAEARGGNGLLVDYGRFERFNAPAELIFEVPAQGIAEDGTFSLALARDYLDD
                     VEVEQVTPEPDRVRADGEWLTFSFASATPQTVTVVFHLQHDRIGPTELRVRLGDGEPM
                     AIGQFIYP"
     gene            2400..2843
                     /locus_tag="VHK28_07160"
     CDS             2400..2843
                     /locus_tag="VHK28_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX2142035.1"
                     /translation="MDAVLRAIAIYAILLIVFRIAGKRALSQITTFDFVLILVIGEAT
                     QQALLGSDFSITNAALVIVTLLGLDIGLSLLVSKVPALQQVVEDVPLVIVEDGRMLKE
                     RLAKSRVDVTDIMEEARRLQGLERMEQIKYAVLERNGGISIIPKG"
BASE COUNT          521 a          995 c         1067 g          422 t
ORIGIN      
        1 cctagaaggc ccacgccccg ctgctgaagc gcagggcgaa gtacacgaac aggcccagcg
       61 acaccgtcga gatggcgacc agcacgcctg cgcggcggct gacgagtgcc atggccacga
      121 acagcgggaa gagggtcagg ctgtagcgcg ggaccgagac caggaagccg gtcgaggtga
      181 acatcagcca gttgccggcc atccaggcga accacgacgg gcggaaccgc agggcggcgt
      241 acaccgtcgc cgccaggccg agcgcggtga aggcgagctc catgcccccg tacatcacca
      301 tggtgtccac gttgcgcgag ccgaaccagc ggaaggcggt ggcgatggcc acccacggcg
      361 gctccaacga cttgaaccag tgcgactcct ggaccgcgag gaaatggaag gcatcgccgt
      421 agaccgcctg gttcagccca agataggcgg ctggaccgat gagcaccagc ccgaccgcca
      481 gccaccgcag gcggaagcgc cgctcaccgg ccggctgctc gaaccactgc gtggcggcct
      541 cggccggcag cgccagcagc aggaccaggc cgttcacccg ggtcagggca gccagcgcac
      601 ccagcaggcc ggccagccac catcggtcag tgcgcgccgc caggaaggag ccgagcacca
      661 gggccaggaa cagcgcctcg gtgtacccga tgtgcaggaa gaaggcggtt gggaagatga
      721 ggcacagcca cgccgcgcgg agggcgatgc ccggcccctc ctcgtggcgc accaggcggt
      781 agagcagggg agccacgaac aggctggcca cgctgctgac cacgaaggcc gagaccagcg
      841 gctggttgac gaccgcgttg acggccgtgg ccagccatgg gtagagcggg tagaagacga
      901 tgtagagcgg caggtcgccg ggaaagacgg accgataccc tccggggccg accaggttcc
      961 ccggatcgac ggcgcggtag ccgaacacga tgaggtccag gtagtgcggg gcgtcccagt
     1021 cgttccacgg gcgcagcacg tccagcgggc cgctgatgtc gccgcggacg atgaccaggt
     1081 gggcgatggc ccccagcccc agcacgccga gcttgaccag cagggtcagc ccgaccagca
     1141 gggactcggg ttgccggagc cgatgcattg gcgcggagtg taggcggcgc cggaacaccg
     1201 ttatctatcg gccaggaagc ggctgccggc ggtcgccatc gaccggttcc ggagcggctg
     1261 gtagactatt ggctcccatc ccacggatgg cccggtcggg gcgtggcgca gcctggtagc
     1321 gcgcagcgtt cgggacgctg aagtcggagg ttcaaatcct ctcgccccga ccaactcatc
     1381 ttcagtcgtc cgccatcacc cgccgctcgc ccaccggccg agtcgcctcc gggaaggcca
     1441 tcgagttcgc cgccacccac cactccatga cgtcgtaggc gaggcgcccg gcctgcaccg
     1501 acggatccgc atctgacagg cgcctggcct cgtcgatgtc gcaggcgaag atcgaaagcc
     1561 cgcgcatggt cacgtccgac tgctctccga acgggccgtt tgcgacgatg accccctcgt
     1621 tccgcagccg tgcacggtag gccaggtgca gcgcctggag cgcgtggagc tcggcctcgg
     1681 acatttccgg cgcgtcgggc gggcgtcgga gcaggacgag cgtgtagacg tcgaacgcat
     1741 ccgggatgtt cgggtctcga ggcacgtccg aaaggatagt ggcccgacgt ggcccctgac
     1801 ttccgattcg cgactgggag cgcacgctcg ctgagcgggc tggcacggcg gctgctttac
     1861 ccagcgcccg aaaggagcct gctgtgtcgg tgcaccgggt gggggatctg gaagtcgagc
     1921 aggacctggc cttccagcgt cgcgaatgga tcgccgagcg gatcggttgg acggccatgc
     1981 tggtcctggc cgtggccggt gtgctggggc tgttcgggac gggtccgctg gcagcggccg
     2041 aggcgcgcgg cggcaacggg ctgctcgtcg actacggccg cttcgagcga ttcaacgctc
     2101 cggcggagct catcttcgag gttccggcgc aggggatcgc ggaggacggc accttcagcc
     2161 tggccctggc ccgggattac ctggacgacg tcgaggtcga gcaggtgacg ccggaaccgg
     2221 acagggtgcg ggccgacggg gaatggctga ccttcagctt tgccagcgcg acaccccaga
     2281 ccgtcaccgt cgtcttccac ctgcagcatg accgcatcgg tccaaccgag ctccgcgtgc
     2341 ggctggggga cggcgagccc atggccatcg gccagttcat ctacccgtag gaggcctcaa
     2401 tggatgccgt gctccgcgcg atcgccatct acgcgatcct gctcatcgtc ttccgcatcg
     2461 cgggcaagcg ggcgctgtcg cagatcacca ccttcgactt cgtgctgatc ctggtcatcg
     2521 gcgaggcgac ccagcaggcg ctgctgggga gcgacttctc catcacgaac gcggcgctgg
     2581 tcatcgtgac gctgctcggc ctggacatcg gtctgtcgct gttggtctcc aaggtgccgg
     2641 cgcttcagca ggtggtggag gacgtgccgc tggtgatcgt ggaagacggg cggatgctca
     2701 aggagcggct cgcgaagtcg cgcgtggacg tcaccgacat catggaggag gcgcgacggc
     2761 tccagggcct ggagcgcatg gagcagatca agtacgccgt gctggaacga aacgggggca
     2821 tctccatcat tcccaagggg tagatggacc gatgccccgt ttcggagtgt ccccggccgg
     2881 atcacgtcga tccggcgcgg gaacgtgaag gtcaggcgcc ttcggcgggc tgaaccctga
     2941 ccgcgcgcgg tcccttggga ccctgctcgg tgtcgaagct cactgcctct ccaccgacca
     3001 gccga
//
LOCUS       DATACA010000348         7748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1970913, whole genome shotgun sequence.
ACCESSION   DATACA010000348 DATACA010000000
VERSION     DATACA010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970913"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VHK28_07165"
     CDS             complement(<1..332)
                     /locus_tag="VHK28_07165"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2142036.1"
                     /translation="MRLTITLTTTGRRSGQPRSVTLYAFRVPGSGALVVIGSYGGAAR
                     DPAWAGNLRASPAATVREGRAELAVTAREVQGEERDRLWQLAVDDFPLYATYERKTRR
                     TIPVFLLEP"
     gene            complement(469..544)
                     /locus_tag="VHK28_07170"
     tRNA            complement(469..544)
                     /locus_tag="VHK28_07170"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Arg,seq:gcg)
     gene            complement(558..650)
                     /locus_tag="VHK28_07175"
     tRNA            complement(558..650)
                     /locus_tag="VHK28_07175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Ser,seq:gct)
     gene            752..1627
                     /locus_tag="VHK28_07180"
     CDS             752..1627
                     /locus_tag="VHK28_07180"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142037.1"
                     /translation="MQRLPVIGQSFTNSAFEAVQRAAIEGSGLDVAIERWERRPHLLG
                     DAIAELRGQEFIGALVVQPHKERAAPLMGALSDDARSTGAVNVIVRDGSRLRGHNTDV
                     DGVRAGLRAILPKGQVNWPKSALVLGAGGGARAVVAVLIGSGFQRVMLFNRHLHRAEA
                     VATHFARVARHMELRARPWHETFLESELSKSGLLVNASGIGVEDGSSPIPAELLPENL
                     HVLDLVLNHQTTPLMRDAQARGGTVANGQLSFLAASAEAFKLLTGREASLDVMRAALA
                     DELGIPEEGIAVVGD"
     gene            1646..2227
                     /locus_tag="VHK28_07185"
     CDS             1646..2227
                     /locus_tag="VHK28_07185"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003346845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX2142038.1"
                     /translation="MFYTTGWVHVICGCMFCGKTDEMLRLLRRFSIAGRSVILVKPRL
                     DTRTDEATVVSRSGAQHDAVTVDDATQIPGVVDDADIVAIEEGQFWDERLPDVVEQLA
                     DSGKQVVVTGLDRDFRGVPFGAMPRLMAMADQVTKLTAICMVCGEPATRTQRLIDGEP
                     ASSDSPLIVIGGMGDDRYEARCRLHHEVPPRDS"
     gene            2314..2763
                     /locus_tag="VHK28_07190"
     CDS             2314..2763
                     /locus_tag="VHK28_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142039.1"
                     /translation="MRFVITLLTAMALVLVACDMGASTPTEQPIPTVQGTDDGAMASA
                     GASADATSDDDDDNGDDDDSSPIAMTCEEAVSDIEDDVSSLTDLESITDELDDTIGAC
                     ASVQEWEDAVSEVAPGLTLTDVESFIDERCDDNDRIDDTPICEEVDD"
     gene            complement(2983..3070)
                     /locus_tag="VHK28_07195"
     tRNA            complement(2983..3070)
                     /locus_tag="VHK28_07195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3034..3036),aa:Ser,seq:tga)
     gene            complement(3216..4487)
                     /gene="serS"
                     /locus_tag="VHK28_07200"
     CDS             complement(3216..4487)
                     /gene="serS"
                     /locus_tag="VHK28_07200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2142040.1"
                     /translation="MIGLQQIRDDPDGVRAAVARKGEPREAVDGVRQADERRRALEAE
                     VNELRAQRNTGNKEMGTLMRDGRRGEADALKARMAELSQRIDALSADLEAANQAIEAS
                     LLLIPNLPHPSVPDGKGPEDNPVIRTWGEPTGEGTAPPHWEIAERLALFDLERGAKIS
                     GSGFIVYTGAGARLQRALIGLMLELATEAGYTEIWSPLLVNAASARGTGQLPDKEGQM
                     YAVERDDLYLIPTAEVPVTNLYRDEILSAERLPIRHASYTPCFRREAGAAGKDTRGLL
                     RVHQFDKVEMVKFVHPSTSYEELESLTTDAEAVLQALELPYRVVERCTRDVGFAQAKG
                     YDLEAWAPGVRKWLEVSSASNYTDFQARRMNLRFRAAADARPELVHTLNGSGLGMSRT
                     YAALLETHLQADGSVRIPERLRPHFGADAIR"
     gene            complement(4484..6085)
                     /gene="lysS"
                     /locus_tag="VHK28_07205"
     CDS             complement(4484..6085)
                     /gene="lysS"
                     /locus_tag="VHK28_07205"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX2142041.1"
                     /translation="MYWADELAARVSGPQVVNDSKTPSGTVHVGSLRGPVILDAIARA
                     LRDRDLETTLLYGVDDLDPMDAQALLTPDAVDRYMGVPLAHVPDPGDDGHASYARHFA
                     GLFIDTFVGLGIHPDRYYWMSELYAAGDMDRYLRVALDRAQLVREIYRRVSRVERPEG
                     WLPVHVICPRCGRVGTTLARDWDGETVAFECLPDLVEWARGCGNGGRLAPFGGSAKLP
                     WNLEWAAQWSHFGVTIEPCGKDLATRGGSRDRSDAIAREVFEREPPLNVPYEFLNIGG
                     RKMSTSKGRGAAAHRIAEVVPAEQLRLLFLRPRPNQAIDWDPEGTDAIPRLFDESDRI
                     AAAAAGREVKGELPPDPQRLHALTLVDADVDAAGEAAAYRPPFAHLAMLVQVPGVDLH
                     ARVEAEKGSPLTSREGEILEERVRAARAWLEAYAPDRARIEIRRDALPQAVAELDAAQ
                     RRAMGTLAGGLPEHWDGEALQASIFEAAKATGLPAGPVFIALYVSFLGKPHGPRAGWL
                     LASLDRDFVVERLREAAAADTLDPE"
     gene            complement(6123..6584)
                     /gene="greA"
                     /locus_tag="VHK28_07210"
     CDS             complement(6123..6584)
                     /gene="greA"
                     /locus_tag="VHK28_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX2142042.1"
                     /translation="MNNKPVYLTAEGLEKLKVELNHLITVERPRVAARIHDAKLDGDI
                     SENAEYEDAKQEQSFLEGRIATLEAQLKNAEVIAGTNGDKVGIGSKVVIKGDDGEETF
                     TIVGSAEAAPREGRISNESPVGAALMGRKKGDKVTVQAPAGSVIYTVVRVG"
     gene            complement(6866..7711)
                     /locus_tag="VHK28_07215"
     CDS             complement(6866..7711)
                     /locus_tag="VHK28_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2142043.1"
                     /translation="MELVFQAVLIGVLQGLTEFIPISSSAHLELAPWIFGWEGDGLIG
                     SLAFDVFLHLGTLAALIGYFWRDWVRLARAFFASIAERRIADDADRRLAWLLALGTVP
                     AAVIGYLGEDLIDEVFHGGSDPIRLAIAGFLVVGALALLAADRLGARRRELHDVGVGT
                     TLAVGFSQALALLPGISRSGATITAALALGLTREAAARFSFLLATPITLGAGLYGSRD
                     LLSEPHSGTEWIAIASGFGASAVAGALAIGLLLAWLRRRSVAVFSLYRLAFAALVVVL
                     VVAGR"
BASE COUNT         1140 a         2719 c         2733 g         1156 t
ORIGIN      
        1 ggctccagca ggaagacggg gatggtgcgc cgcgtcttgc gctcgtaggt ggcgtacagc
       61 gggaagtcgt cgaccgccag ctgccagagc cggtcgcgct cctctccctg cacctcgcgg
      121 gcggttaccg cgagctctgc gcgcccctcc ctgaccgtgg ccgccgggct cgcgcgcagg
      181 ttgccggccc acgccgggtc ccgcgctgcc ccgccgtagg aaccgatgac caccagcgcg
      241 ccgctaccgg gcaccctgaa ggcatagagg gtcacggaac ggggctggcc gctccgccgg
      301 ccggtcgtgg tcagggtgat ggtgagacgc atgcggaatg ctcccgggcg atgggcgcgg
      361 ctacgatggc acagtcgtcc gcgaccggtg gacatcggtc cgttgcgggc cggctggaat
      421 cggtctcggt ctaggtgcgc atgcctcgct cgtagctacc gaggcgagtg gcgtgcccga
      481 gaggactcga acctccgacc ttcaggtccg caacctgacg ctctatccac tgagctacgg
      541 gcacacgcga gggatcgtgg cggagaggga gggattcgaa ccctcggaag aggtttaccc
      601 ccttcaacgg tttagcaaac cgccgcacta ggccactatg cgacctctcc gaagaccgcc
      661 ccgatgatag catggggtca ccggagcgac gaacgagcgc catcctcggc gcgccgtcgc
      721 gcagccacca ttctgccatc gagccgacgt cgtgcagcgc ctacccgtca tcggtcaatc
      781 cttcaccaat tccgccttcg aggccgtgca gcgagcggcc atcgaagggt ccgggctgga
      841 cgtcgccatc gagcgctggg aacggcggcc gcacctgctg ggcgacgcca tcgcggagct
      901 gcgcggccag gagttcatcg gtgcattggt cgtccagccg cacaaggagc gcgccgcgcc
      961 cctgatgggc gccctctcgg acgacgcccg gtcaaccggc gccgtcaacg tcatcgtgcg
     1021 ggacggcagc cggctgcgcg gccacaacac ggatgtagac ggcgtgcgcg ccggcctgcg
     1081 ggcgatcctg cccaaggggc aggtcaactg gccgaagagc gccctggtgc tgggtgccgg
     1141 cggcggggcg cgggcggtgg tggccgtcct catcggttcg ggtttccagc gggtgatgct
     1201 cttcaatcgt cacctgcacc gggccgaggc ggtcgccacc cacttcgcgc gcgtcgcgcg
     1261 ccacatggag ctgcgcgccc ggccctggca cgagaccttc ctggagtcgg agctctcgaa
     1321 gtccgggctg ctggtgaatg ccagcggcat cggcgtcgag gacgggtcca gccccattcc
     1381 ggcagagctg ctgccggaga acctccacgt cctggacctc gtgctgaacc accagaccac
     1441 tccgttgatg cgtgacgcac aggctcgcgg cggcacggtg gccaacgggc agctctcgtt
     1501 cctggccgcc tcggccgagg ccttcaagct gctcaccggc cgggaggcgt cgctggacgt
     1561 gatgcgcgcg gcactggccg atgagctagg catccccgag gaggggatcg ccgtggtcgg
     1621 cgactgacgg gggctggcgg gcgccgtgtt ctacaccacc ggctgggtcc acgtcatctg
     1681 cggctgcatg ttctgcggca agaccgatga gatgcttcgc ctcctgcgcc gcttctcgat
     1741 cgccggtcgc agcgtgatcc tggtcaagcc tcggctcgac acgcggaccg atgaggccac
     1801 cgtcgtctcg cgctccggtg cccagcacga cgcggtcacc gtggacgacg ccacccagat
     1861 ccccggggtg gtggacgacg ccgacatcgt ggccatcgag gagggtcagt tctgggacga
     1921 gcggttgccg gacgtggtcg agcagctggc tgactcgggc aagcaggtgg tggtcaccgg
     1981 cctcgaccgc gacttccgcg gcgtgccgtt cggcgccatg ccacggctga tggccatggc
     2041 cgaccaggtc accaagctga ccgccatctg catggtgtgc ggggagccgg caacccggac
     2101 ccagcgcctg atcgacggcg agcccgcctc ctccgactcg ccgctgatcg tgatcggcgg
     2161 catgggcgac gatcgctacg aggcgcgctg ccggctgcac cacgaggtcc cgccgcgcga
     2221 ctcctgatcg gccgctgtcc tcgtatgggc acggtgcttg cgttccggtt ctccccgaca
     2281 tccaacgaga tacgttcttc caggggagac aacatgcgat tcgtgattac cctgctgacc
     2341 gccatggcgc tggtgctggt cgcctgcgac atgggcgcca gcacgcccac cgagcagccg
     2401 atacctaccg tccagggcac cgacgacggt gccatggcaa gtgccggtgc cagcgcggac
     2461 gccacgtcgg acgatgacga cgacaacggt gacgatgacg actcgtcgcc gatagccatg
     2521 acctgcgagg aggcggtctc ggacatcgag gacgacgtct cgtccctcac cgacctcgag
     2581 tccatcaccg acgagctcga cgacaccatt ggcgcctgcg catcggtcca ggaatgggag
     2641 gacgcggtca gcgaggtggc gcccggcctg accctgaccg atgtcgagtc attcatcgat
     2701 gagcgctgcg acgacaacga ccggatcgac gacacgccga tctgtgagga ggtcgacgac
     2761 tgactccggt cgacccgtgc gatcgaggcc ctggcagcgc tcgccggggc ctcgtcgtat
     2821 ccgataaaag ggggccgaag cggtttcccg cctcggcccc tgacgtcacc cgagctcgtg
     2881 gtccggccct gcagggcttt ctgaggagcc tgtacggtcc cccaccggcc acgaagtcga
     2941 gatacaacca gggggtgcac gatgcgtgcc gactcgttct ggtggcggag ggggagggat
     3001 tcgaaccccc ggaggcttgc acctcagcgg ttttcaagac cgccgcaatc gtccgctctg
     3061 ccacccctcc gctggtccat tctaagcacg aaccgggccg acggctgtcc ggcgtggtgg
     3121 agtgccccca ctccggacgc gttcggcaga gcatgggcag ccacgggctg cacgtggagg
     3181 agcaagcggg ccgggcacgc gggccggagc ccctctcacc ggatggcgtc ggctccgaag
     3241 tggggacgca ggcgctcggg gatccggacg ctgccatccg cctgcaggtg ggtctccagg
     3301 agcgcggcgt aggtccggct catgcccagt cccgagccgt tgagcgtgtg caccagctcg
     3361 gggcgggcgt cggctgccgc ccggaagcgc aggttcatgc gccgcgcctg gaaatcggtg
     3421 tagttggagg cggagctgac ctccagccac ttccggacgc cgggtgccca cgcctcgagg
     3481 tcgtagccct tggcctgcgc gaagccgacg tcgcgcgtgc agcgctccac aacgcggtag
     3541 ggcagctcca gggcctggag cacggcctcg gcatcggtcg tgagggattc cagctcctcg
     3601 taggaggtgg acggatggac gaacttgacc atctccacct tgtcgaactg gtggacgcgc
     3661 agcagcccgc gagtgtcctt gccggcggcg cccgcctccc gacggaagca gggcgtgtag
     3721 gacgcgtggc ggattggtag acgctcggcg gacaggatct cgtcgcggta caggttggtg
     3781 accggcacct cggcggtggg gatgaggtac aggtcgtctc gctcgacggc gtacatctgt
     3841 ccctccttat ccggcagctg tccggtgccc cgtgccgatg cggcgttgac cagcagcggc
     3901 gaccagatct cggtgtagcc cgcctcggtg gccagctcga gcatgagacc gatgagcgcg
     3961 cgctgcaggc gcgcgccggc cccggtgtac acgatgaacc ccgagccgga gatcttggcc
     4021 ccccgctcca ggtcgaacag cgccaggcgc tcggcgatct cccagtgcgg cggcgcggtc
     4081 ccctcccccg tcggctcgcc ccaggtgcgg atgaccggat tgtcctccgg ccccttccca
     4141 tccgggaccg atgggtgggg caggttcggg atgagcagca ggctggcctc gatggcctgg
     4201 ttggccgcct cgaggtccgc ggacagggca tcgatgcgct gcgacagctc ggccatgcgc
     4261 gccttgaggg catcggcctc cccgcggcgg ccatcgcgca tcagggtgcc catctccttg
     4321 ttgccggtgt tgcgctgcgc ccgcagctcg ttgacctccg cctccagcgc ccgccgccgc
     4381 tcgtcggcct gacgaacgcc gtcaactgcc tcccggggct cgcccttgcg ggccaccgcc
     4441 gccctgacgc cgtcggggtc gtcgcgaatc tgctgcaggc cgatcattcc gggtcgaggg
     4501 tatcggccgc ggcggcttcc cggagccgct ccacgacgaa atcgcggtcc agactggcaa
     4561 gcagccagcc ggcgcggggg ccgtggggct tgccaaggaa cgagacgtag agcgcgatga
     4621 agacggggcc cgccggcagg ccggtcgcct tcgccgcctc gaagatggag gcctgcagcg
     4681 cctcgccgtc ccagtgctcg ggaaggccgc ccgccagtgt gcccatcgcc cgtcgctgcg
     4741 cggcgtccag ctcggccacg gcctgtggca gcgcgtcgcg gcggatctcg attcgggccc
     4801 gatccggggc gtacgcctcc agccaggcgc gggcggcacg cacccgctcc tcgaggatct
     4861 caccctctcg ggacgtgagc ggcgagccct tctcggcctc gacgcgggca tgcaggtcga
     4921 cgccgggcac ctgaacgagc atggccaggt gggcgaacgg cgggcggtag gcggctgcct
     4981 ctccggcggc gtccacgtcg gcgtccacca gggtcagcgc gtgcaggcgc tgcgggtccg
     5041 gcggcagttc gcccttcacc tcgcgccccg cggcggcggc cgcgatgcgg tccgactcgt
     5101 cgaagaggcg tggaatggca tcggtgccct ccgggtccca gtcgatggcc tggttaggcc
     5161 ggggtcgcag gaagaggagg cgcaactgct cggcgggcac cacctcggcg atgcgatgcg
     5221 ctgccgctcc ccgacccttg gaggtggaca tcttcctgcc gccgatgttc aggaactcgt
     5281 acggcacgtt gagcggaggc tcccgttcga agacctcgcg ggcgatggcg tccgaccggt
     5341 cgcgtgaccc gccgcgcgtg gcaaggtcct tgccgcaggg ctcgatcgtc accccgaagt
     5401 ggctccactg cgcggcccac tccaggttcc agggcagctt ggcgctgccg ccgaacggcg
     5461 ccaggcggcc gccgttgccg cagccacgcg cccactcgac caggtccggc aggcactcga
     5521 aggcgaccgt ctctccgtcc cagtcgcggg ccagcgtggt gcccacccgg ccgcagcgcg
     5581 gacagatgac gtggacgggc agccagccct ccgggcgctc cacgcggctg acgcggcggt
     5641 agatctcgcg caccagctgc gcacggtcga gggccacccg gaggtatcgg tccatgtcac
     5701 cggcggcgta cagctcgctc atccagtagt agcggtccgg gtggatgccg aggccgacga
     5761 aggtgtcgat gaagaggccc gcgaagtggc gggcgtagga ggcgtggccg tcgtcccccg
     5821 ggtctgggac gtgcgccagc ggcacgccca tgtatcggtc caccgcgtcc ggcgtcagca
     5881 gcgcctgggc gtccatggga tccaggtcgt ccacgccgta gagcagtgtg gtctccagat
     5941 cgcgatcgcg cagggcgcgg gcgatcgcgt ccaggatgac cgggccacgc agcgagccca
     6001 cgtggaccgt gcccgaaggg gtcttggagt cgttgacgac ctgtggaccg gagacgcgcg
     6061 ctgcgagctc gtcagcccag tacacggaag gtgcgagcgc cggagggcgg gcccggacgg
     6121 ccctacccga cccggacgac ggtgtagatg accgacccgg ccggtgcctg gacggtgacc
     6181 ttgtcgccct tcttgcggcc catcagcgcg gcgccgaccg gcgactcgtt gctgatgcga
     6241 ccctcgcgcg gagcggcctc ggccgagccc acgatggtga aggtctcttc gccgtcgtcc
     6301 cccttgatga ccaccttgga accgatgccg accttgtctc cgttggtgcc ggcgatcacc
     6361 tcagcgttct tgagctgcgc ctcgagcgtg gcgatgcggc cctccaggaa cgactgctcc
     6421 tgcttggcgt cctcgtactc ggcgttctcg ctgatgtctc cgtccagctt cgcatcgtgg
     6481 atgcgcgcgg cgacccgcgg ccgctcgacg gtgatcaggt gattcagttc caccttgagc
     6541 ttctccagcc cttcggcagt caggtagaca ggcttgttgt tcatcccgat ccaccccctc
     6601 tccaggtcgt gtcagggccc cggctcggcg gggccgattc gcttggcgcc gcggggtgac
     6661 ggcggcaaag cgtccccctg tctgacgtaa caaaaaacgc tccaggcccc gccattgcgt
     6721 cgtcgctggc gtccctgaag ctggccgctg ccagtgcgtt ggcccgtcgg cgtgccgttg
     6781 cggatcaccg acgccgcgcg attgtaggcc gggagcgtgt gggtgtcaaa ggcggatggg
     6841 ccttcgcggc tcccggatca gcccctcaac ggcccgccac caccagcacc accaccaggg
     6901 cggcgaaggc cagccggtac aggctgaaga cggccaccga gcggcgccgc agccaggcca
     6961 gcaggagccc gatggccagt gccccggcca ccgccgacgc cccgaagcca ctggcgatgg
     7021 cgatccactc cgtcccgctg tgcggctccg acagcaggtc tcgcgagccg tagagcccgg
     7081 cgcccagggt gatgggcgtg gcaagcagga agctgaagcg ggcggccgcc tcccgggtga
     7141 gccccagggc cagcgctgcg gtgatggtgg cgcccgatcg gctgatgccg ggcagcaggg
     7201 ccagcgcctg gctgaagccg accgccagcg tggtgccgac ccccacatca tgcagctccc
     7261 gccgccgcgc gccgagccgg tcggctgcca gcagggccag ggcgccgacc accaggaagc
     7321 cggcgatcgc caggcggatg gggtccgatc cgccgtggaa gacctcgtcg atcaggtcct
     7381 cgcccaggta gccgatgaca gccgccggca ccgtgcccag cgccagcagc caggccaggc
     7441 ggcggtcggc gtcgtcagcg attcggcgtt cggcgatgct ggcgaagaag gcgcgtgcca
     7501 ggcggaccca gtcgcgccag aagtatccga tcagcgccgc cagggtcccc aggtgcagga
     7561 agacgtcgaa ggccaggctg ccgatcagcc cgtcgccctc ccagccgaag atccatggcg
     7621 ccagctccag gtgcgcgctg gagctgatgg ggatgaactc ggtcagcccc tgcaggacac
     7681 cgatcaggac cgcctggaag accagctcca tgcgtcagga ccgatgggtg cccggcgcgg
     7741 cgggtgag
//
LOCUS       DATACA010000349         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1167609, whole genome shotgun sequence.
ACCESSION   DATACA010000349 DATACA010000000
VERSION     DATACA010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167609"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..423)
                     /locus_tag="VHK28_07220"
     CDS             complement(94..423)
                     /locus_tag="VHK28_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142044.1"
                     /translation="MTTSKTFAHDAVLTMGPEADLRAPGAAITVALCGHWEHEPPCPL
                     AAHHTAAARDGELLRLRILFACEPADEAEVRRRVGAALAARWTVTASGPSAVRPDEAA
                     HVARLAG"
     gene            592..2085
                     /locus_tag="VHK28_07225"
     CDS             592..2085
                     /locus_tag="VHK28_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019030235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX2142045.1"
                     /translation="MNTTGWLKGLRVTADGTGIVSHAGVALIRALVDNVGLTAGLSRA
                     LASDRWLVHDRGRVLADLACAIADGAEVISDFRVIGDQEDLFGLVASVPTAWRTLDEI
                     ARGGERALGRITTAVNAARCRAWAATAARHGAIPGIRVADKTLEGVTCIRLDASVVGC
                     HSDKEGAEPNFKGFGLHPLLAYCDNTAEPLAGMLRPGSAGSNTAADHLAVLDAAIIAL
                     PAAFRRRLMVTCDGAGASHDLITQLDALAARPGYQVLYSVGWELGKREKAALTAVPTQ
                     AWHIAVDEHGEVRERRANDACGDWSCGHRRCWIEEAHVTELTALLRCGRAGDQLAGWP
                     TTMRVFARRERPHPGAQLSLFETEDGWRYSLWVTNLPERTPGWRGQAAYIDAAHRGHA
                     RVEDAIRTGKDCGIGKYPSTSLAMNKAWQAAALTAATLLAWFKLLALDGTLARAEPKT
                     LRYRILHAAARLTRGARQRRLNIQATWPWAADIITAWERISALPQAP"
     gene            2248..>2434
                     /locus_tag="VHK28_07230"
     CDS             2248..>2434
                     /locus_tag="VHK28_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142046.1"
                     /translation="MIWVDDEQYGSGPDGEFIAGVTEIVPLAVLDTPQGTRIKFLGAP
                     TEEAIAVPAHLDHADRFF"
BASE COUNT          378 a          880 c          841 g          335 t
ORIGIN      
        1 cgctggacga cagcggcgcg ctggcggcga gcctgccgct gtaggcgagg cggagccggc
       61 gtctgccgcg ctgtgcgggg cgctggtcgc ggctcagccg gccagccggg ccacgtgcgc
      121 cgcctcgtcc ggccgcacgg ccgacggccc ggacgcggtg accgtccacc gggcggccag
      181 cgccgccccg acccgccgcc gcacctcggc ctcgtcggcc ggctcgcacg cgaacaggat
      241 gcgcagccgc agcagctccc cgtcgcgggc cgcggccgtg tggtgggcgg cgagcgggca
      301 cggcggctcg tgctcccagt gcccgcacag cgcgaccgtg atcgctgcgc cgggagcccg
      361 caggtcggcc tccgggccca tggtcagcac cgcatcgtga gcgaacgtct ttgatgtcgt
      421 catttgtcgg tgatgttcgg ccgtcttcga ggtagtgtcg agacggctaa ccctgagaat
      481 tcacgtcgcc gtgtgagctg accggtgtgg cagcaatgga aagtgctcct gacctgggac
      541 gataagggtt gctgagatcc gtatccgccc agatcatgga gcactttatc ggtgaatact
      601 accggctggc tgaagggttt gcgggtcacc gcagacggca cggggatcgt gtcgcatgct
      661 ggggtagcgc tgatcagggc gctggtggac aacgtcggcc tgaccgcagg gctgtcacgg
      721 gcgctggcgt cggaccggtg gctggtgcat gaccggggcc gggtgctggc ggacctggcg
      781 tgtgcgatcg cggacggcgc cgaggtgatc agtgactttc gggtgatcgg tgaccaggag
      841 gatctgttcg ggctggtcgc ctcggtgccc acggcgtggc ggacgctgga tgagatcgcc
      901 cgcggcgggg agcgggcgct gggcaggatc accaccgcgg tgaacgccgc ccggtgccgg
      961 gcgtgggcgg cgacggccgc ccggcacggc gccatccccg gcatccgcgt cgcggacaag
     1021 acgctggagg gcgtgacctg catccggctg gacgccagtg tggtgggctg ccactcggac
     1081 aaggaaggcg ccgagccgaa cttcaaggga ttcggcctgc acccgttgct ggcctactgc
     1141 gacaacacgg ccgaaccact ggccgggatg ctgcgccccg gctcggcggg cagcaacacc
     1201 gccgccgatc acctcgcggt gctggacgcc gcgatcatag cgctgccggc ggcattccgg
     1261 cggcggctga tggtcacctg cgacggcgcc ggggccagcc acgacctgat cacccagttg
     1321 gatgccctcg ccgcccggcc cggctaccag gtcctctact cggtcggctg ggagctgggc
     1381 aagcgggaga aggccgcact caccgccgtc ccgacccagg cctggcacat cgcggtggac
     1441 gaacacggcg aggtccgcga gcggcgcgcc aacgacgcct gcggcgactg gtcctgcgga
     1501 caccgccggt gctggatcga agaggcgcac gtcaccgaac tgaccgcact gctgcgctgc
     1561 ggccgggccg gtgaccagct tgccggctgg cccaccacaa tgcgggtgtt cgcccgccgc
     1621 gagcgccccc accccggcgc ccaactgtcg ctgtttgaga ccgaagacgg ctggcggtac
     1681 tccctgtggg tcaccaacct gcccgaacgc accccaggct ggcgcggcca ggccgcctac
     1741 attgacgccg ctcaccgggg acacgccagg gtggaagacg ccatccgcac cggcaaagac
     1801 tgcgggatcg gcaagtaccc ctccacctcg ctggcgatga acaaagcctg gcaggccgcc
     1861 gccctgaccg ccgcgaccct gctcgcttgg ttcaagctgc tcgccctgga cggcaccctc
     1921 gcgcgcgccg agccgaagac cctgcgctat cgcatcctgc acgccgccgc ccggctgacc
     1981 cgcggcgccc gccagcgccg cctcaacatc caagccacct ggccctgggc cgctgacatc
     2041 atcaccgcct gggaacggat cagcgccctg ccgcaagccc cctgaccacc agcaaaccgt
     2101 ccctgcgatc acggaaggaa gccccggggc ccagggaacc cccggccacc cagcccgaca
     2161 gccgggccac cgtcataccc cgaccctaaa atcaagatcc actatccggc gcggcagcca
     2221 tctcacgcca gcaaccggtc accgtgaatg atctgggttg acgacgagca gtacggctcc
     2281 ggcccggatg gcgaattcat cgcaggagtg acagagattg ttcctctcgc ggtcctggat
     2341 acgccccagg gaacccggat caaattcttg ggagccccga ccgaagaagc gatcgccgtg
     2401 cccgcccatc tcgaccacgc cgatcgcttc ttcg
//
LOCUS       DATACA010000350         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2774267, whole genome shotgun sequence.
ACCESSION   DATACA010000350 DATACA010000000
VERSION     DATACA010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774267"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1435
                     /gene="tig"
                     /locus_tag="VHK28_07235"
     CDS             62..1435
                     /gene="tig"
                     /locus_tag="VHK28_07235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX2142047.1"
                     /translation="MVTVSTRPEPGSRMVLEIEVPPAEVDRHFATAYRHVAERTKVPG
                     FRPGKAPRHVIDRFVGRASVLAEAIDHLVDEAYDAAVDQASIFPIDQPQVDIDPATVG
                     EGQAVRFTATVAVRPEVELGAYTEYPFGLEIPEVTDEQVEEVIGQLRQDQATLRPVDE
                     RGAQEGDVAAVKFVGTIDGEPFEGGSADRLPLVIGEGGMIPGFEDQLSGMKKDETKDF
                     DLTFPDDYRVEELRGKQAHFEVTLLDLRERQLPELDDDFARSVGEVGSVDELRTEIRD
                     ALAKRAEAEARHTFADRIIDFASSNASVELPEVMVANEIEIMRDELRNRLAAQRIGME
                     QYLQLAKQTPEELNTELREPAARRVKTLLVLSAIAEKEGVDVSPDEIQAEIDDQLARY
                     GNDAKLAEYLGSTRGRAYLRMTLRNRKLVDTLIDRALGTDASASTTEASSAPEDADPA
                     PTTESQE"
     gene            1441..2118
                     /gene="clpP"
                     /locus_tag="VHK28_07240"
     CDS             1441..2118
                     /gene="clpP"
                     /locus_tag="VHK28_07240"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX2142048.1"
                     /translation="MLVPMVIESSARGERAFDIYSRLLKERIIFLGDAIEDHIANLVI
                     AQLLFLESEDPEKDISLYINSPGGVVTSGLAIYDTMQYLKAPVSTICIGQAASMGAVL
                     LAAGTTGKRYALPNSRIMIHQGSGGFRGNTPDVEIQVKEMLHLTDRLMHILAKHTGQE
                     YEKVKHDSERDYFMSAEEAKAYGLVDEVFAGNTESLISMAQEAGGKMPTSDEAAEADA
                     APEPKKK"
     gene            2130..2543
                     /locus_tag="VHK28_07245"
     CDS             2130..2543
                     /locus_tag="VHK28_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142049.1"
                     /translation="MGRVLALAAALILAGCATQPSGAPTSFFFPRHGDPLGAGDAALL
                     EGEVVFADACLWVRSGNGTLFLPLWPADVVLGKINDRPAVLSADRELLVETGEVTMLG
                     GSQTDRATAEQLVGPIPDRCAGGPFWNVSTVSNRP"
     gene            complement(2728..3195)
                     /locus_tag="VHK28_07250"
     CDS             complement(2728..3195)
                     /locus_tag="VHK28_07250"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2142050.1"
                     /translation="MLHAFGTLETPIGLLWVVVSSAGLVSITRGELPPAGAEADAGAV
                     APVLDELDAYFAGQLREFSVDLDLTGAAAFDAEVWTAARGIRYGETVSYGELAMIAGH
                     PAAARAVGGSLARCPVAPVVPCHRVIHADGSIGGWGSEPWVKRWLLDLERGGS"
     gene            3267..>3920
                     /gene="clpX"
                     /locus_tag="VHK28_07255"
     CDS             3267..>3920
                     /gene="clpX"
                     /locus_tag="VHK28_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX2142051.1"
                     /translation="MTSKTKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINLCQEII
                     EEEMLEAPRASKSQATPLPSPRKIKEKLDDYVISQEQAKKVLSVAVYNHYKRVNAGHT
                     VDDVELGKSNVMLVGPTGSGKTLLAQTLAKVLDVPFCIADATSLTEAGYVGEDVENIL
                     LRLIQAADFDVQRAQRGIIYIDEIDKIARKTDNPSITRDVSGEGVQQALLKILEGTVA
                     "
BASE COUNT          714 a         1333 c         1345 g          528 t
ORIGIN      
        1 tgtcgcaaga cgtgcgagtt cgaatctcgc ctcgcccacc aacccttgtc aggagctgca
       61 cttggtcacc gtctccacgc ggcccgagcc cggatccagg atggtcctcg agatcgaggt
      121 gccgccggcc gaggtcgacc gccacttcgc gaccgcctac cggcatgtcg ccgagcgcac
      181 gaaggtgccc ggcttccggc ctggaaaggc cccgcgccac gtcatcgacc gcttcgtggg
      241 ccgcgcctcg gtgctggccg aggcgatcga ccacctcgtg gacgaggcgt acgacgccgc
      301 ggtcgatcag gcgtccatct tccccataga ccagccgcag gtggacatcg atccggcgac
      361 ggtcggtgag gggcaggccg tgcgcttcac cgccaccgtg gccgtgcggc ccgaggtgga
      421 gctgggcgcc tacaccgagt accccttcgg tctcgagatt cccgaggtca ccgacgagca
      481 ggtcgaggag gtcatcgggc agctgcgcca ggaccaggcc accctgcgcc cggtcgacga
      541 gcgcggcgcg caggagggcg acgtggccgc cgtcaagttc gtgggcacca tcgacggcga
      601 gccgttcgag ggtgggagtg ccgatcggct gccgctggtg atcggcgagg gcggcatgat
      661 ccccggcttc gaggaccagc tgtccgggat gaagaaggac gagaccaagg acttcgacct
      721 gacgttcccc gacgactatc gggtcgagga attgcgcggc aagcaggccc acttcgaagt
      781 cacgttgctc gacctgcgcg agcggcagct cccggagctg gacgacgact tcgcgcgctc
      841 ggtgggcgag gtcggcagcg tggacgagct gcgcaccgag atccgggacg ccctggccaa
      901 acgggcagag gcggaggcgc gccacacctt cgccgaccgg ataatcgact tcgcctcgtc
      961 gaacgccagc gtcgagctgc cggaggtgat ggtcgccaac gagatcgaga tcatgcgcga
     1021 cgagttgcgc aatcgcctgg cggcccagcg catcggcatg gagcagtacc tgcagctggc
     1081 caagcagaca ccggaggaac tcaacacgga gctgcgcgag ccggcagcgc gccgggtgaa
     1141 gacgctgctg gtcctgtcgg ccatcgccga gaaggagggg gtcgacgtca gcccggacga
     1201 gatccaggcc gagatcgacg atcagctggc ccgctacggc aatgatgcca agctggccga
     1261 gtacctcggc tccacgcgcg gccgcgccta cctgcgcatg accctccgca accgtaaact
     1321 ggtcgacacc ctgatagatc gcgcgctcgg gaccgatgcc tccgcctcga cgactgaggc
     1381 atcatcggct cccgaagacg ccgaccccgc gccaaccact gaatcccagg agtaacccgc
     1441 atgctggtcc ccatggtcat cgagtcgagc gcccgcggcg agcgggcatt cgacatctac
     1501 tcgcggctcc tcaaggagcg catcatcttc ctgggggacg cgatcgagga tcacatcgcc
     1561 aacctggtga tcgctcagct ccttttcctc gagagcgagg acccggagaa ggacatcagc
     1621 ctgtacatca actcgccggg tggcgtggtc acctccggcc tggccatcta cgacacaatg
     1681 cagtacctga aggcgccggt gagcacgatc tgcatcggtc aggcggcctc gatgggcgcg
     1741 gtgctgctgg ctgcggggac gaccggcaag cgctacgcgc tgcccaacag ccggatcatg
     1801 atccaccagg gctcgggcgg cttccgcgga aacacgccgg acgtggagat ccaggtcaag
     1861 gagatgctcc acctcaccga ccggctgatg cacatcctgg ccaagcacac cggccaggag
     1921 tacgagaagg tcaagcacga ctcggagcgt gactacttca tgagcgccga ggaggcaaag
     1981 gcctacggcc tggtggacga ggtcttcgcg ggcaacaccg agtccctgat cagcatggcc
     2041 caggaggccg gtgggaagat gccgacctct gacgaggccg cggaggcaga cgccgccccg
     2101 gagcccaaga agaagtaagg gccggtggcg tgggccgcgt cctggcactg gccgcggccc
     2161 tgatcctggc aggctgcgcc acgcagccga gcggggcgcc cacctcgttc ttcttcccgc
     2221 gtcacggtga cccgttgggg gcaggggacg ccgcgctcct cgagggagag gtggtgtttg
     2281 ccgatgcctg cctctgggtg cgcagcggga acggcacgct gtttctgccg ctgtggccgg
     2341 ccgacgtggt gctgggcaag atcaacgacc ggcccgccgt cctgagcgcc gatcgggagc
     2401 tgctggtgga gaccggcgag gtcaccatgc ttggcgggag ccagacggac agggccacgg
     2461 cggagcagct cgtggggccc atcccggacc gatgcgccgg cggacccttc tggaacgtca
     2521 gcaccgtcag caaccggccc tgacaccctt gcgcggtatg gctggcgaag ccgtgaattc
     2581 gccagattca cgcgaggtgc gcaccggggc cgccgagctg ggcgcggtgc cgccaccctg
     2641 caggttgggg gacacacatg gcgatgaccg ccgccctacg acgtgcgcgc gtgcgcgtca
     2701 tctgaatctg gcgaagcgag ggacccgcta cgagccaccg cgctcaaggt ccagcagcca
     2761 gcgcttgacc cagggctccg acccccagcc gccgatcgag ccgtcggcgt ggatcacgcg
     2821 gtggcagggc accaccggcg cgaccggaca gcgggccagg gagcctccaa cggctcgcgc
     2881 cgccgcagga tggccggcga tcatcgccag ctccccgtac gagaccgtct cgccgtaccg
     2941 aatgccccgt gcggcagtcc agacctccgc gtcgaaggcg gcggcacccg tcaggtccag
     3001 gtccacgctg aattcgcgca actggccagc gaagtaggcg tccagctcgt ccagcacggg
     3061 tgccaccgcg ccggcatccg cctccgcccc ggccggtggc agctcgccac gggtgatgga
     3121 caccaggccg gcggaggaga ccacgaccca caggagcccg atgggcgtct caagggtgcc
     3181 aaacgcgtgg agcactgcgg gggatgctag cgccggacca tcggtgcagt cgggcggctg
     3241 cggagcgcgt gctatcctcg gaaacgatga ccagcaagac caaggtcccg taccgctgct
     3301 cgttctgcgg caagagccag gagcaggtcc gcaagctgat cgccgggcag ggcgtctaca
     3361 tctgcgacga gtgcatcaac ctctgccagg agatcatcga ggaggagatg ctcgaggcgc
     3421 cgcgcgccag caagtcgcag gcgacgccgc tccccagccc gcgcaagatc aaggagaagc
     3481 tggacgacta cgtcatcagc caggagcagg cgaagaaggt cctctcggtc gcggtctaca
     3541 accactacaa gcgggtcaac gccggccaca ccgtggacga cgtggagctc ggcaagagca
     3601 acgtcatgct ggtggggccc acgggaagcg gcaagaccct gctggcccag acgctggcca
     3661 aggtgctcga cgtcccgttc tgcatcgcgg acgccaccag cctgacggag gccggctacg
     3721 tcggcgagga cgtggagaac atcctgctgc gcctgatcca ggccgccgac ttcgacgtgc
     3781 agagagccca gcgcggcatc atctacatcg acgagatcga caagatcgcg cgcaagacgg
     3841 acaacccgtc catcacccgc gacgtatccg gcgaaggcgt gcagcaggcc ctgctcaaga
     3901 tcctggaggg gacggtggcc
//
LOCUS       DATACA010000351         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2774278, whole genome shotgun sequence.
ACCESSION   DATACA010000351 DATACA010000000
VERSION     DATACA010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2774278"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1519)
                     /locus_tag="VHK28_07260"
     CDS             complement(14..1519)
                     /locus_tag="VHK28_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX2142052.1"
                     /translation="MLANTFSASLLGVEGLPVRVEVDVAYGLPSLTIVGLAGSQVQEA
                     RERVRSALRNSGFELPARRITVNLSPADLPKDGTGYDLAIAVGILAASGQLPDQSRLE
                     GVALLGELALDGGLRPIVGAMSLVAAARDDGRRAVIVAEADAPEAACVTVVAALPASR
                     LGEAVGYLAGVRQLIPATAEPSASVLPALDGAPDLADVMGQPAPRRSLEIAVAGRHNL
                     ALCGPPGVGKTLILRCAQSLQPPLDDIEALEVARIHSAAGLLDRRAPLNRRRPFRAPH
                     HTVSTQGLVGGGPRVRPGEASLAHRGILMLDEALQFRADALDGLRQPLEAGMVTIARA
                     EGAVSLPARFALLIAFNPCACGWRGSTTRECHCDDAAARRYAARLSGPLRDRIDLWVR
                     AEESPGAAVDGVEPSAAVAERVQAAWARQLERQSVANADLPLTGLAERVRLGGHVASA
                     VEQRGQRFLLSPRRLHRLMRVARTIADLDASETVEARHVDEALYYRPEVAA"
BASE COUNT          247 a          624 c          594 g          198 t
ORIGIN      
        1 cttcccacgc cgatcacgcc gccacctccg ggcggtagta gagcgcctcg tccacgtgcc
       61 gggcctcgac cgtctcggac gcatccaggt cggcgatggt gcgagccacg cgcatcagcc
      121 gatgcagccg ccgcggcgac agcaggaaac gctgcccccg ctgctccacc gcggacgcga
      181 cgtgtccgcc caggcgaacg cgctccgcga ggccggtaag cggcagatcg gcattcgcca
      241 cgctctgccg ttcgagctgt cgcgcccacg cggcctgcac ccgctccgcg accgccgcgc
      301 tgggctccac gccatccaca gcggctccgg gtgactcctc ggcacggacc cacaggtcga
      361 tccggtcgcg cagcggcccg gagaggcgcg cggcgtagcg gcgggcggcc gcatcgtcgc
      421 agtggcattc gcgcgtcgtg ctgccccgcc agccgcaggc gcacgggttg aaggcgatca
      481 gcagcgcgaa gcgggccggc agcgaaacgg cgccctcggc ccgagcgatg gtgaccatcc
      541 ccgcctcgag cggctggcgg aggccgtcga gcgcatcggc ccggaactgg agtgcctcgt
      601 ccagcatgag gatgccgcga tgcgccaggg atgcctctcc cggtcgcacg cgtggaccgc
      661 cacccaccag gccctgtgtg gagacggtgt ggtggggcgc ccggaacgga cggcggcggt
      721 taagtggggc tcggcggtcg agcaggccgg ccgcggaatg gatgcgcgcc acctccagcg
      781 cctcgatgtc gtccagcggc ggctggagcg actgcgcgca gcgcaggatc agcgtcttgc
      841 ccacaccggg tggaccgcac agcgccaggt tgtggcgccc ggcaaccgcg atctcgagcg
      901 accggcgcgg tgccggctgg cccattacgt ccgccaggtc cggagcgcca tccaatgccg
      961 gcagaaccga tgcggacggc tccgccgtcg ccgggatgag ctggcggacg ccggccaggt
     1021 agccgaccgc ctctcccagg cgagatgcag gaagcgcagc caccacggtc acgcatgccg
     1081 cttcgggcgc gtccgcctcg gcgacgatga ccgcccgacg cccgtcatcg cgtgccgccg
     1141 caaccagcga catggcgccc acgatcggcc gcagcccgcc gtccagggcc agctcgccga
     1201 ggagggcaac cccctccagg cgcgactggt ccggcagctg cccggaagcg gccaggatgc
     1261 cgaccgcgat ggcaaggtcg tacccggtgc cgtccttggg caggtcggct ggcgacaggt
     1321 tgacggtgat gcggcgcgcc ggcagctcga agccagagtt gcgcaatgcg ctccgcaccc
     1381 gctcccgcgc ctcctgcacc tggctgccgg ccaggcccac gatggtcagc gaggggaggc
     1441 cgtaggcaac gtccacctcc acccgaacgg gcagtccctc gacccccagg agcgaggcgc
     1501 tgaaggtgtt ggcgagcacg gcgacacctc tgagcgaggc gagcgcgggt gagcgaggta
     1561 gtgccggccg cccgcctgct cgcctcccaa actccagcgg cgaccggcgt gccggagcgt
     1621 aggagaccgg gccttatcgg ccccttatca gccggttatc agc
//
LOCUS       DATACA010000352         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1971478, whole genome shotgun sequence.
ACCESSION   DATACA010000352 DATACA010000000
VERSION     DATACA010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971478"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VHK28_07265"
     CDS             <1..1017
                     /locus_tag="VHK28_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142053.1"
                     /translation="FGGSVLRRPEDIAAAVAVVAEQRASGLRPVVVVSAFEGVTDTIL
                     ESAAVLLPGGRDALAGPPHDPGSPLARETDRALATGEALSAALFALGLVARGIPARSF
                     SGAEAGIRTAAAHLGAEVRRVVPRLLRQAIADGMVPVVAGFQGIGTHGELTTLGRGGT
                     DLSAVALAVALRAVRCALFKDVGGVMEADPHLVPGARFLPAVDALQLELLAEMGAEVV
                     HPVAVRRARRGRLHLLVRSTDGVQPQTEVRPGQPRPRVGALELPPMLAVARRERVARI
                     SLVGPARLLPDILPIPAHGAGSFDEGPMRVVWAEVPLEQAAPAAQAVHAAVMLSRPRP
                     APEP"
     gene            complement(1022..1984)
                     /locus_tag="VHK28_07270"
     CDS             complement(1022..1984)
                     /locus_tag="VHK28_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2142054.1"
                     /translation="MTSNDRDAAPDLVGAGLVLMAAACFGTLGPLSRFAGDAGVESLT
                     VVTWRAALGAALVGAFLLGRAAAGRGRPVSLRAIPVRDRWFILAAACANTLLNLGAFI
                     AFERITIALTLLVFYLYPALVAVISTVWFGERLDRLRWGALVLSLVGMALVVFQAPEG
                     GRFDLLGIGLAFVAALMQVFYVLAARHGFAHVPGAQAGAMTMGGATVLYVLIALGLGN
                     VGQLAQPTASLDALWPVVMAGTLGAGIPTVAYILGIRRLGPSRAAILATFEPVVGVLL
                     AALLLAERPAPIHLVGGALIIASGVVLQLRSRAKVAEHEAVSGD"
     gene            complement(1981..3999)
                     /locus_tag="VHK28_07275"
     CDS             complement(1981..3999)
                     /locus_tag="VHK28_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX2142055.1"
                     /translation="MSLVTLSGIAKSHGAQHLFEGVDLQVGAGRRLAIVGPNGAGKTT
                     LLEIITGEQEADEGSVARARDLVIGYLRQEVAESRGQTAIAEVLTGAGAVSGIERRMR
                     HIEAELSEATQEDELAELMDEYGRLQHRFEAMGGYGLEAEARRILAGLGFAEADMDRN
                     TAEFSGGWMMRISLARLLLQNPDLLLLDEPTNHLDLASVEWLQGFLAEYAGAVILVSH
                     DRDFINAIVNRVAELNHGQFTEYVGDYADFVEQRETQIEQLERQAAVQGRKVAQIERF
                     IERFRYKNTKARQVQSRIKQLDRMDRVEVPSDRTKSVKFRFPQPPRSGRTVITLDGIV
                     KRYGQHTVYDGLDLVLERGQKVALIGPNGAGKSTLLKILAGVLPFEGGRRELGTNVRV
                     AYFAQHQIDALNPANTVFAELASVAPRLSTAEMRRLLGAFLFSGEAVEKPVEVLSGGE
                     QTRLALAKLMADPANLLCLDEPTNHLDIASRDVLEDALNAFPGTIVLITHDRYLIRSV
                     ANAIIEVNAGEATVFPGDFEYYAAKRGLDIEQRGAVEGKATPRGIEGPVARPRESASA
                     AAARRRSEAEQRNRRYRRTHDLRAALERVEAEASRNDAELAQVTERLSDQAVYADAEA
                     VRQLIERHNAARDRAEALAAEWASVSAELEAVERDGAGDPGPVSAGRR"
     gene            complement(3996..4916)
                     /gene="thrB"
                     /locus_tag="VHK28_07280"
     CDS             complement(3996..4916)
                     /gene="thrB"
                     /locus_tag="VHK28_07280"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2142056.1"
                     /translation="MPLLASVDVPASSANLGSGFDCFAAALSLKLRAELHHGDEAGVV
                     LNAHGEGAPGPDDDPRQNLLLRAFREGVRAALGSPAGSGSWRIEVSSMIPAARGLGSS
                     AAVIVAGLLLGASVGRRAPDADELLRLATDIEGHGDNVAAALYGSFTLAVGGTLRRFR
                     VPEGWIPILFVAAAHSRTTEMRRALPEMVPHADAARQAGRSALLATALITGDASLLRE
                     AMADTLHQPYRLPLLPGAGPLIDAAYEHGAAGAALSGAGPSVLAICDSPAAAHSVEKA
                     WNASGVAGMATRLRFDASGARLTHREEAAG"
     gene            complement(5297..5908)
                     /locus_tag="VHK28_07285"
     CDS             complement(5297..5908)
                     /locus_tag="VHK28_07285"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX2142057.1"
                     /translation="MNRTNTLMVLPDTRVRCPWAGDDPLYVAYHDTEWGVPLRDDPAL
                     FELLNLEGAQAGLAWITILRKRDGYRRAFAGFDPEVLAAWGDEEETRLLADPGIVRNR
                     AKVRAVFGNARAYLRLTDETGSFAGHLWSVVDGAPIQNRWARMDQVPAETDVSRALSA
                     DLRRRGFSFVGPTIVYAFMQSAGLVNDHLVDCFRHAEVARPAG"
BASE COUNT          851 a         2195 c         2249 g          899 t
ORIGIN      
        1 ttcggcggct cggtgctgcg gcgaccggag gacattgcag cggcggtcgc ggtcgttgcc
       61 gagcagcgcg cgtccgggct gcgtccggtg gtcgtcgtct cggccttcga gggcgtgacc
      121 gataccatcc tcgagtcggc cgcggtcctg cttcccggtg gccgtgacgc gctggccggt
      181 ccaccgcacg accccggctc gccgctcgcc agggagacgg accgcgccct ggccaccggc
      241 gaggcgctct cggcggcgct cttcgcgttg ggtttggtgg cccgcggcat cccggcacgc
      301 tccttcagcg gggcggaggc cggcatacgc accgcggccg cccacctggg tgccgaggtt
      361 cggcgcgtgg tgccccgcct gctgcgccag gccatcgcgg acggcatggt cccggtggtg
      421 gccggcttcc aggggatcgg cacccacggt gagctgacca cgctgggtcg gggcggcacc
      481 gacctctcgg cggtggcgct ggcggtggcc ctgcgcgcgg tgcgctgcgc gctgttcaag
      541 gacgtcggtg gcgtgatgga ggccgacccg cacctggttc ccggcgcccg cttcctgccc
      601 gcggtcgacg cgctgcagct ggagctgctg gccgagatgg gcgccgaggt ggtgcacccg
      661 gtcgccgtgc gccgtgcgcg tcgtggacgc ctgcacctgc tggtgcgctc gaccgatggg
      721 gttcagcccc agaccgaggt ccgcccgggc cagccgcggc cacgtgtcgg tgcgttggag
      781 ctgccgccca tgctggcggt ggcgcgccgc gagcgggtgg cgcgcatcag cctggtgggt
      841 cccgcgcgcc tgcttccgga catcctcccc attccggcgc acggagccgg cagcttcgac
      901 gagggaccga tgcgcgtggt gtgggccgag gtgccactgg agcaggccgc gccggcggca
      961 caggcggtcc acgccgccgt catgctgagt cgtccccgac cggcgccgga gccctgatga
     1021 gtcagtcccc ggacaccgcc tcgtgctcgg cgaccttggc ccgcgagcgg agctgcaaca
     1081 ccacgccgga ggcgatgatc agcgcgccgc cgaccaggtg gatgggcgcc ggccgctcgg
     1141 ccagcagcag ggcggccagc aggacgccga ccaccggctc gaaggtggcc aggatggcgg
     1201 cgcgcgatgg gcccaggcgg cggatgccca ggatgtaggc gacggtcggg atcccggcac
     1261 ccagggtgcc ggccatgacc actggccaca gcgcgtcgag cgaggccgtc ggctgtgcca
     1321 gttggccgac gttgcccagc cccagcgcga tcaggacgta caggacggtg gcgcccccca
     1381 tggtcatggc ccctgcctgc gcgccgggca cgtgggcgaa gccgtggcgc gcggccagga
     1441 cgtagaagac ctgcatgagg gccgccacga acgcgagacc gatgcccagc aggtcgaagc
     1501 gcccaccctc cggtgcctgg aagaccacca gcgccatgcc gaccagcgag agcaccagag
     1561 cgccccagcg cagccggtcg agacgctccc cgaaccagac cgtggagatc accgccacca
     1621 gggcgggata gaggtagaag accagcaggg tgagcgcgat ggtgatgcgc tcgaaggcga
     1681 tgaaggcgcc caggttgagc agggtgttgg cgcaggccgc ggccaggatg aaccatcggt
     1741 cccggaccgg gatggcccgc agggagaccg gccgcccacg gccggcagcg gccctgccga
     1801 gcaggaaggc gccgaccaac gccgccccaa gcgccgcccg ccaggtcacc accgtcagcg
     1861 actcgacgcc cgcgtcgccg gcgaagcggc tcaatggccc cagggtgccg aagcacgcgg
     1921 cggccatgag caccaggccg gcaccgacca ggtccggtgc ggcgtcgcgg tcgtttgaag
     1981 tcatcgccgg ccggcggaca ccggcccggg atcacccgcc ccgtcgcgct cgacggcctc
     2041 cagctccgcc gagaccgatg cccactcggc ggcgagcgcc tcggcgcgat ctcgggcggc
     2101 gttgtggcgc tcgatcagct ggcgcacggc ctccgcatcg gcgtagacgg cctggtcgga
     2161 gaggcgctcg gtgacctggg ccagctcggc gtcattgcgg ctcgcctcgg cctcgacgcg
     2221 ctccagcgcg gcgcgcaggt cgtgggtgcg ccggtagcgg cggttgcgct gctcggcctc
     2281 ggaccggcgg cgcgcggcgg cagccgacgc cgactcacgc ggacgggcaa ccggtccctc
     2341 gatcccgcgc ggagtggcct tgccttccac ggccccgcgc tgctcgatgt ccagtccgcg
     2401 cttggcggcg tagtactcga agtcgcccgg aaagacggtc gcttcgccgg cattgacctc
     2461 gatgatcgcg ttcgccacgc tgcggatcag gtatcggtcg tgggtgatga gcacgatggt
     2521 gccggggaat gcgttcagcg catcctccag cacgtcccgc gacgcgatgt ccaggtggtt
     2581 ggtgggctcg tccaggcaca gcaggttggc tggatcggcc atcagcttgg ccagggccag
     2641 ccgtgtctgc tctccgcccg agagcacctc cacgggcttc tcgacggcct cgccgctgaa
     2701 caggaaggct cccagcaggc ggcgcatctc ggcggtggac aggcgcggcg ccacgctggc
     2761 cagctcggcg aagacggtgt tggccgggtt gagcgcatcg atctggtgct gggcgaagta
     2821 ggcgacccgc acgttggtgc ccagctcgcg ccggccgccc tcgaagggca gcacgccggc
     2881 caggatcttg agcagcgtgc tcttgccggc gccgttggga ccgatgaggg ccactttctg
     2941 gccgcgctcc agcaccaggt ccaggccgtc gtacacggtg tgctggccgt agcgcttgac
     3001 gatgccgtcc agggtgatca ccgttcgccc gctccgcggc ggctgcggga aacggaactt
     3061 gaccgacttg gtgcgatcgg acggcacctc cacccggtcc atgcggtcca gctgcttgat
     3121 gcggctctgc acctggcgcg ccttggtgtt cttgtagcga aagcgctcga tgaagcgctc
     3181 gatctgcgca acctttcggc cctggacggc cgcctggcgc tccagctgct cgatctgcgt
     3241 ctcgcgctgt tccacgaagt ccgcgtagtc gccgacgtac tcggtgaatt ggccgtggtt
     3301 cagctcggcc acccggttga cgatggcgtt gatgaagtcg cgatcgtgac tgaccaggat
     3361 cacggcaccg gcgtactccg ccaggaatcc ctgcagccac tccaccgacg cgaggtccag
     3421 gtggttggtc ggctcgtcca gcaggagcag gtccgggttc tgcagcagca gtcgcgccag
     3481 ggagatgcgc atcatccagc cgccggagaa ctccgcggtg ttgcggtcca tgtccgcctc
     3541 cgcgaagccc agcccggcca ggatgcggcg ggcctccgcc tccagcccgt agccgcccat
     3601 ggcctcgaac cgatgctgga gccgcccgta ctcgtccatc agctccgcca gctcgtcctc
     3661 ctgggttgcc tcggacagct ccgcctcgat gtgacgcatg cggcgctcga tgccgctcac
     3721 cgcgccggcg ccggtcagca cctcggcgat cgccgtctgg ccgcgcgact cggccacctc
     3781 ctggcgcagg tagccgatca ccagatcgcg ggcgcgcgcc acgctgccct cgtccgcctc
     3841 ctgctccccg gtgatgatct cgagcagggt cgtcttgccc gcaccgttcg gacccacgat
     3901 agccaggcgg cgtccggcgc ccacctgcag gtccaccccc tcgaacaggt gctgcgcgcc
     3961 atggctcttc gcgatcccgg acagcgtcac caggctcatc cggcggcctc ctcgcgatgg
     4021 gtcaggcgcg cgccgctggc gtcgaagcgc aggcgggtcg ccatgccggc cacacccgag
     4081 gcgttccacg ccttctccac cgagtgggcc gccgccgggg agtcgcagat ggccagcacg
     4141 ctgggtccgg cgccggacag cgccgccccg gccgcgccgt gctcgtaggc ggcgtcgatc
     4201 agcgggccgg caccgggcag cagcgggagg cggtagggct ggtgcagcgt gtcggccatc
     4261 gcctcacgca ggagcgaggc gtcgccggtg atcagcgccg tggccagcag ggcgctgcgg
     4321 cccgcctgcc gcgcggcgtc ggcgtgcggc accatctcgg gcagcgcgcg ccgcatctcg
     4381 gtcgtgcggc tgtgagcggc agccacgaac aggatcggga tccagccctc cgggacgcgg
     4441 aagcgccgca gcgtcccgcc cacggccagg gtgaagctgc cgtacagggc ggcggccacg
     4501 ttgtccccgt ggccctcgat gtcggtggcc agtcgcagca gctcgtcggc gtccggcgcg
     4561 cggcggccga cactggcacc cagcagcagc ccggccacga tgaccgctgc ggagctgccc
     4621 agtccgcgcg ccgccgggat catgctgctt acctcgatcc gccacgagcc gctgccggcc
     4681 ggcgagccga gcgccgcccg cacgccctcg cggaaggccc gcagcagcag gttctggcgc
     4741 ggatcgtcat cagggccggg agcaccctcg ccgtgggcgt tgaggacgac ccccgcctcg
     4801 tcgccgtggt gcagctcggc gcgcagcttg aggctgagcg cggcggcgaa gcaatcgaag
     4861 ccgctgccca ggttggcact gctggccggg acgtcgaccg aggcgaggag gggcatgcgg
     4921 accgagtata ggaacgtggc gccggacatg aagatagcgg cccgtccgtt tgggacgggc
     4981 cgctatcggt gcggaaggaa cgtctgcggt gcaggcatgc ggcggttcag ttgaagagcc
     5041 ggcgcaggcg tgcagcaacg cccttggggg gacgctgcga ccgagaatct cgggtcttca
     5101 ggagcatctc ctgcgctcga acctcggcca tcagctgcat ggtcacggga tgcacttctg
     5161 tggcctcctt tccctcgccg ccgcattcct tgcggccgtc gcgaactgag cacagtgtgc
     5221 cgccggccgc tccccgtggt gagcggcatg cgtcccgact tcgcctacgt cattgcgccg
     5281 agcggcgccg agtcgctcag cccgccgggc gggccacctc cgcgtggcgg aaacagtcga
     5341 ccaaatggtc gttgaccagc ccggcggact gcatgaaggc gtagacgatg gtgggtccca
     5401 cgaagctgaa gcctcgtcgc cggaggtctg cggagagggc ccgggagaca tcggtctcgg
     5461 cggggacctg gtccatgcga gcccaccggt tctggattgg cgcgccgtcg acgacggacc
     5521 acaggtggcc ggcgaatgag ccggtttcat cggtcagccg gaggtaggcc cgcgcgttgc
     5581 cgaacacggc ccgcaccttg gcccggttgc gcacgattcc cggatcggcc agcagtcgcg
     5641 tttcctcctc gtcgccccag gcggccaaca cctcgggatc gaagccggcg aaggcgcggc
     5701 ggtagccgtc acgcttgcgg aggatggtga tccacgccag cccggcctgg gcaccctcca
     5761 ggttgagcag ctcgaacagg gcggggtcgt ctcgtagcgg gacgccccac tcggtgtcgt
     5821 ggtaggccac gtagagcggg tcgtcaccgg cccacgggca gcgcacgcgg gtgtcaggga
     5881 ggaccataag ggtgttcgtc ctgttcatcg cttcaggctg gtggtgccgt cagtctcggg
     5941 cacgcactgc ctcctcgcgg ctgatgatga ccgatggatc cctggtccgg tttggcgctg
     6001 cggacaagag acgggcagct tttgacgtga cgggtgcgcg gcaggcatga ggggcgaagt
     6061 tcttcgcagt ccacgtccag cgcagccaat atatgaggca gttcttcacg ggggctgggg
     6121 ctgcgtgtca gtctgccgat acagcgggca ggtccctgca cgaagcgaca agttcttcgc
     6181 ccctcacgct cacg
//
LOCUS       DATACA010000353         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1650149, whole genome shotgun sequence.
ACCESSION   DATACA010000353 DATACA010000000
VERSION     DATACA010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650149"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VHK28_07290"
     CDS             complement(<1..537)
                     /locus_tag="VHK28_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142058.1"
                     /translation="MSDPVARRRGWDSLLTRAWWLHPFLFAAVPIVFLFAYNIREQIS
                     LDPLLEPLGMALAGAAGVLVLLTVLALALKRDPARAALVASLAIGLFMTYGHVWNSVS
                     GLLGIHRNLLLAWGVIGLVGTVLAMRARAGAVRGATAGLNVIGLVLLAINVVPIPDHQ
                     LRVASVVAGQADPSVSGEV"
     gene            complement(570..860)
                     /locus_tag="VHK28_07295"
     CDS             complement(570..860)
                     /locus_tag="VHK28_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142059.1"
                     /translation="MSALELLESLRAPEAAAPDRRTEGEQVMVEFPMRFGATTLRLRR
                     MAGRWLASAPAPDGELRLAGDASPYLAASLALEPWQVDASHVMRVVATALRS"
     gene            complement(857..2128)
                     /locus_tag="VHK28_07300"
     CDS             complement(857..2128)
                     /locus_tag="VHK28_07300"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2142060.1"
                     /translation="MRSVAPVEVPQGEALRSALSQFDRVADYLNLDSEIAAILRVPKR
                     EWTVRFPVRRDSGDVEVFTGYRVQHNVARGPAKGGIRFHPSTDLDEVRALAMWMTWKC
                     ALVNVPFGGAKGGVTVDPQTLSADELPRVARRFTAELQGIIGPDVDIPAPDMGTNAQT
                     MAWMMDTYSMHAGYSVPGVVTGKPIALGGSEGRADATGMGVAITVAHAMRKLGRRLEG
                     ATVAVQGFGNVGEAAARLLAERGAKIVAITDVGGGVQNPDGLDLAQLKRQLEETGTIA
                     GAPHTRPIDNPELLALDCDVLVLAALEGQLTGDNVGDVRARLIAEGANGPTTPEADAA
                     LYARGIPVIPDILCNAGGVVVSYFEWVQNLQSYHWQPAQVAERLEGVMATAFDEVWQI
                     ADERKIDNRLAALSVAVGRVALAQRLRGIYP"
BASE COUNT          345 a          831 c          784 g          303 t
ORIGIN      
        1 cacctcccca ctgaccgatg gatcggcctg cccggccacc acggacgcca cccgcagctg
       61 gtggtcgggg atgggcacca cgttgatggc cagcagcacc agcccgatga cgttcagtcc
      121 cgccgtcgcg ccccgcacag cgccggctcg cgcccgcatg gccagcacgg tcccgaccag
      181 gccgatgacg ccccaggcaa gcagcaggtt gcggtggatg ccgagcaggc cgctgaccga
      241 gttccagacg tgtccatagg tcatgaacag cccgatggcc aggctggcca cgagggccgc
      301 gcgcgccggg tcgcgcttca gcgccagggc cagcacggtg agcagcacca gcacgcccgc
      361 cgcgccggcc agggccatcc ccagcggctc gagaagcgga tcgagcgaga tctgctcgcg
      421 gatgttgtag gcgaacagga agacgatggg gaccgccgcg aagaggaacg ggtgcagcca
      481 ccacgcgcgc gtcagcagcg agtcccaccc tcgtcgacgc gcgaccggat cggacatcag
      541 gcgccgagtg tagccgtgac gcccctacgt cagctcctca gcgcggtggc cacgacccgc
      601 atcacgtgag aggcgtcgac ctgccacggc tcgagggcca gggacgcggc caggtacggc
      661 gaggcatcgc cggccagccg cagctcgccg tcgggcgcgg gtgcgctggc cagccagcgg
      721 ccggccattc ggcgcaggcg gagggtcgtg gccccgaacc gcatcgggaa ctcgaccatc
      781 acctgctctc cctctgtccg ccggtcaggg gccgccgcct cgggcgcgcg cagcgactcg
      841 agcagctcca gggcactcat gggtagatgc cccgcagccg ctgggcgagg gccacgcggc
      901 ccaccgccac cgacagcgcg gccagccggt tgtcgatctt ccgctcgtcg gcgatctgcc
      961 acacctcgtc gaacgcggtg gccatcacgc cctccagccg ctcggcgacc tgcgccggct
     1021 gccagtggta ggactggagg ttctggaccc actcgaagta gctgaccacc acgccccccg
     1081 cgttgcacag gatgtccggg atgaccggga tgccgcgagc gtacagcgct gcatcggcct
     1141 ccggagtggt cggaccgttg gccccctcgg cgatcagccg ggcgcgcacg tcgcccacgt
     1201 tgtcgccggt cagctggccc tcgagggccg ccagcaccag tacgtcgcag tcgagcgcca
     1261 gcagctccgg gttgtcgatc ggtcgggtgt gcggagcgcc tgcgatggtg ccggtctcct
     1321 ccagctgccg cttgagctgg gccaggtcca ggccgtcggg gttctgcacc ccaccgccga
     1381 cgtcggtgat ggccacgatc ttcgcgccgc gctcggccag cagcctcgcg gcggcctcgc
     1441 cgacgttgcc gaagccctgc accgccacgg tggcgccctc caggcgtcgc ccgagcttgc
     1501 gcatggcatg cgcgaccgtg atcgccacgc ccatgccggt ggcgtcggca cggccctcgg
     1561 agccgccgag ggcgatcggc ttgccggtca ccacgccggg aacggaatag ccggcgtgca
     1621 tcgagtaggt gtccatcatc caggccatgg tctgggcgtt ggtgcccatg tcgggggccg
     1681 ggatgtcgac gtcgggcccg atgattccct gcagctccgc cgtgaagcgg cgcgccaccc
     1741 gcggcagctc atcggccgac agggtctgcg ggtcgacggt cacgccgccc ttggcgccgc
     1801 cgaacggcac gtttaccagg gcgcacttcc aggtcatcca catcgccagg gcgcgaacct
     1861 cgtcgaggtc ggtggaggga tggaagcgga tgccgccctt ggccgggcca cgcgccacgt
     1921 tgtgctgcac gcgatagccg gtgaagacct ccacgtcacc cgagtcgcgc cggaccggga
     1981 agcggacggt ccactcccgc ttcggcacgc gcaggatggc ggcgatctcc gagtcgaggt
     2041 tcagatagtc ggcgacgcgg tcgaactgcg acagggcgga gcgcagcgcc tcgccctgtg
     2101 gtacctcgac gggcgccacc gagcgcattt gctgaatagc catggaccga tgttggccgc
     2161 cccaccgcaa caccggcctc aagcaccgta aacggcgcat aaattcggct cggctctatg
     2221 ctggagccga tgtcgcacgc accgatgcgc accgccggcc tgg
//
LOCUS       DATACA010000354         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_525514, whole genome shotgun sequence.
ACCESSION   DATACA010000354 DATACA010000000
VERSION     DATACA010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525514"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VHK28_07305"
     CDS             complement(<1..321)
                     /locus_tag="VHK28_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX2142061.1"
                     /translation="MASSATRADAGAPAALDPFPGTQLGALVRRLPAYARLAWGLSRD
                     QRLSGGRRAAVLGAAAYLASPIDLVPGLIPVAGQLDDAAVALLMVGAMLGMGRLLRPT
                     RPQGG"
     gene            complement(296..1711)
                     /locus_tag="VHK28_07310"
     CDS             complement(296..1711)
                     /locus_tag="VHK28_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HEX2142062.1"
                     /translation="MTDRPAISTLGELRASGYLPRSVKEEMRGNLVASLRNGQAFLPG
                     ILGYDDTVLPQLENAVLAGQDVILLGERGQAKSRIARSLVALLDEWTPEVAGAELHDD
                     PMRPVSPYALALVAECGDDTPVSWLHRDLRYGEKLATPDITIADLVGEVDPVKVAEGR
                     YLSDELTIHYGLLPRTNRGIFALNELPDLAERIQVGLLNLMEERDVQIRGYKVRLDLD
                     LFVIASANPEDYTSRGRIITPLKDRFGAQIRTHYPEDPETEIAIVDAEQTRFADETSA
                     SVPVQVPGFMREVVAELSQLARRSSEVSQRSGVSVRVTIANHETLLAAALKRALRLGE
                     PVAAPRISDLPSVVASTAGKIELETLGDEAREDKVIEKLVQRAVVNVFNHHFSIAELV
                     PVIEHFEAGVVAEVGESVPSTAHERLVDQLPELRRAVAQLDVGESPAGVASGVEFVLE
                     GLHLNRRLNKERRGGELRYAR"
     gene            complement(1708..>2397)
                     /locus_tag="VHK28_07315"
     CDS             complement(1708..>2397)
                     /locus_tag="VHK28_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142063.1"
                     /translation="VDFLPDDTVGVFLRGWAQDMSWTDEIGPDRGYLLVNEMSDHLPI
                     YVWGPNARAALRLAGAGYGFGGTMHADSLAEALDCLRTDLRATDADLAGLTIYLQYSA
                     YRTPGGMYRRVEEAWHLRTDASGTLAPVRLAALQGERSPQLTGAQRMPVPPTLPTDGG
                     RSRRPLVHDESAYAVLAASLGLAPDSFEAELAERAAFLAALDARGLCDPASVARAVSR
                     YPDLPAEEPRA"
BASE COUNT          354 a          851 c          862 g          330 t
ORIGIN      
        1 gccgccctgc ggccgggtcg ggcgcaggag gcggcccatg cccagcatgg cccccaccat
       61 cagcagcgcc actgccgcgt cgtccagctg gccggcaacg ggaatcaggc ccgggacgag
      121 gtcgatgggc gaggcgaggt aggccgccgc gcccagcaca gccgcgcgac gaccaccgct
      181 caggcgctga tcccggctca gcccccaagc caggcgcgca taggccggca gccggcgcac
      241 cagcgcaccg agctgggtgc cgggaaacgg gtcgagcgcc gcgggcgcgc cggcgtcagc
      301 gcgcgtagcg gagctcgcca ccgcggcgtt ccttgttcag ccgccggtta aggtgcagcc
      361 cctcgagcac gaactccacg ccgctggcca cgccggccgg cgattcgccc acgtcgagct
      421 gagccaccgc gcggcgcagc tctggcagct ggtccaccag gcgctcgtgg gccgtggacg
      481 gcacgctctc cccaacctcg gcaaccacgc cggcctcgaa gtgctcgatg accgggacca
      541 gctcggcgat gctgaagtgg tggttgaaga cgttgaccac cgcccgctgc accagcttct
      601 cgatcacctt gtcctcgcgc gcctcgtcgc cgagcgtctc gagctcgatc ttaccggccg
      661 tcgaggcgac caccgagggc aggtcactga tgcggggtgc ggccaccggc tcgccgagcc
      721 gcagagcgcg cttcagcgcg gcggccagca gggtctcgtg gttcgcgatg gtgacccgca
      781 cgctgacccc ggagcgctgg ctgacctcgg acgagcgccg ggcgagctgc gacagctcgg
      841 ccaccacctc gcgcatgaag ccggggacct ggaccgggac cgatgccgag gtctcgtccg
      901 cgaagcgcgt ctgctccgcg tcgacgatgg cgatctccgt ctcaggatcc tccgggtagt
      961 gggtgcggat ctgcgccccg aagcggtcct tcagcggggt gatgatccgg ccgcggctgg
     1021 tgtagtcctc ggggttggcg ctggcgatga cgaacaggtc gaggtccagc cgaaccttgt
     1081 agccgcggat ctggacgtcg cgctcctcca tgaggttcag cagccccacc tggatgcgct
     1141 cggccaggtc cggcagctcg ttcagggcga agatgccgcg gttggtacgc ggcagcaggc
     1201 cgtagtgaat ggtgagctcg tccgacaggt agcggccctc ggcgaccttg accgggtcca
     1261 cctcgcccac caggtcggcg atggtgatgt ccggcgtggc cagcttctcg ccgtagcgca
     1321 ggtcgcgatg cagccagctg accggcgtgt cgtcgccgca ctcggccacc agtgccaggg
     1381 cgtaggggct gaccggacgc atcgggtcgt cgtgcagctc ggctccggca acctcggggg
     1441 tccactcgtc cagcagggcg accagcgagc gcgcgatccg actcttggcc tgcccacgct
     1501 cacccagcag gatgacgtcc tgcccggcca gcacggcgtt ttccagctgg gggaggaccg
     1561 tgtcgtcgta gcccaggatc ccgggcagga aggcctggcc attgcgcagg ctggcgacca
     1621 ggttgccgcg catctcctcc ttgacgctgc ggggcaggta gccgctggcg cgcagctcgc
     1681 caagggtcga gatggcgggt cgatcagtca tgcgcggggt tcctcagctg gcaggtcagg
     1741 gtaacgcgaa acggcgcggg ccacggaagc agggtcgcac agcccgcggg cgtccagcgc
     1801 ggcgaggaat gcggcccgct ccgcgagctc cgcttcgaac gagtccgggg ccaatccaag
     1861 gctggccgcc agcaccgcat acgcggattc gtcgtggacc agcggccgcc gcgaacgccc
     1921 accatcggtg ggcagcgtgg gtggcaccgg catgcgctgc gcgccggtca actgcgggga
     1981 gcgctccccc tgcagggcgg ccaggcgcac cggcgccagg gtgccggagg catcggtgcg
     2041 caggtgccag gcttcctcga cgcgccggta catgccgccc ggcgtgcgat acgcggagta
     2101 ctgcaggtag atggtcagcc cggccaggtc ggcatcggtc gcacgaaggt ccgttcgaag
     2161 gcagtccagc gcctcggcca gcgagtcggc gtgcatcgtg ccaccgaacc cgtaccccgc
     2221 tcccgccagg cgcagcgcgg cgcgagcgtt cggcccccag acgtagatcg gcaggtggtc
     2281 gctcatctcg ttgaccagga ggtagccgcg gtccggcccg atctcatcgg tccaggacat
     2341 gtcctgcgcc cagccgcgga ggaagacgcc caccgtgtca tcgggcagga agtcgac
//
LOCUS       DATACA010000355         4785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1410418, whole genome shotgun sequence.
ACCESSION   DATACA010000355 DATACA010000000
VERSION     DATACA010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410418"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VHK28_07320"
     CDS             complement(<1..806)
                     /locus_tag="VHK28_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142064.1"
                     /translation="MATDRALPLPGRAMQPSAEPGGLPRDRRLALAAVAAASVFAVGL
                     GYLVAGGRWEMALVAAAAVPAILLVIRYAFAGILIWLLVMPFIVRLGSEQPSLVFWGV
                     HRLGLPALLVLVLLLHVLGLRNRSFRFHLVDAMLLLFLAAGVFNVLYLSPSPLRGMVT
                     FYDKLAMPILLFWLIRALAPGRRELSHLLAIGAVTVVIQCVIGVMSWVAPSLLPEGWL
                     GRAGERTVGTLGGPAPYTITLVLFSMLAIYWFATITDRRSLQRPLLVGIVL"
     gene            904..1629
                     /locus_tag="VHK28_07325"
     CDS             904..1629
                     /locus_tag="VHK28_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEX2142065.1"
                     /translation="MIISHTHRYVFVELPRTGSTAIRLELRELYDGQPILHKHATYDE
                     FLRQASPEERTYFSFAAIRNPLDDAVSRYFKLRTDHKGRYSGTGNKKRFKLLKRLQDD
                     RMYRFIERTDADFPTFFRRFYVLPHDTWASVTHHRLDFVMRFESLASDFEEALRRIGI
                     QPKRPLPMVNSTASRRRSFDTYYTPDLYPRARRVFGPYMERWGYTFPDGWRVGPPTTL
                     HRAQYRAVNTLARIYWRFLRPYA"
     gene            1639..2583
                     /locus_tag="VHK28_07330"
     CDS             1639..2583
                     /locus_tag="VHK28_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142066.1"
                     /translation="MEPRYVLLASNAYSGSTLLSFLLGAHPNIATVSDVSGARGQHRM
                     DTFRCSCGRLMAEDPFWLEVRDRMRAAGWTDFDLANFRLGFDYGRSPRFNRLRTGTMR
                     WHSLEALRDAAFRAWPGDESRMRSIGARTASLARVVTELSGANVFVDASKERLRAAYL
                     QRYLALDLRVIHLLRDVRAVAASARRHGRSGTSTVAEAASRWAGTNQAITRSLGAVPG
                     AQRTVVRYEDLCARPDQELARLYRFCGVDPDRAPPLEHAPVQHLLGNRVRLANLTDIR
                     LDRRWQEELSPQEQADALRAAGATYPRFYGSGPQASKV"
     gene            complement(2565..3434)
                     /locus_tag="VHK28_07335"
     CDS             complement(2565..3434)
                     /locus_tag="VHK28_07335"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142067.1"
                     /translation="MGSRLESLRGAVRGTPLHLAWRFAVAAVGARREAASLRQVRTYC
                     LFIGHPRSGHSIVGALLDAHPEMVVSDELDALRYVSAGFTRDQVLFLSLRTARHQAAH
                     QRRKLGRGGTYSYHVEGQWQGRFRELRVVGDSRAGWTVRRLSSDPALLRRLERRMRPL
                     QLRFIHVVRNPFDNISTMVIRGGRTLPNAVERYFATCRGLFDLRQAIGPDRLLTMRHE
                     DLISDPRARLAEACDLLEVVATSEYLDACAGVLYARPSRSRSEIEWPPEMRERVDGLI
                     AEFPFLAGYTFDA"
     gene            complement(3434..4393)
                     /locus_tag="VHK28_07340"
     CDS             complement(3434..4393)
                     /locus_tag="VHK28_07340"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142068.1"
                     /translation="MSNRAPQAVAQRPGPLFIVGCARTGSTLLRHLLNRSSRICLASE
                     THFLAGRNGFRAERRLAAVLSAPEVERSAALRRFASDLAGPNGWRWLERNVGPDELIE
                     RLSGTDLSPKEVFRALIGLYAERACADERAEDGIVGEKTPAHLYAVPRLSEWFPRARF
                     IHTMRDPRGIFASQLVRLRQGRWGPKARVGWIPGALIDPLLAPYEAVRTRRAWLDAVR
                     LDEQYRALFGDRYLLVRFEDLVTDPEHELRRVLAFLDLPYDPALLSGAEVVGSSFAAV
                     RHAGQGIDSSAATRWRSRVWRPWRAWFATTLRAELARFGYPAA"
     gene            complement(4390..>4785)
                     /locus_tag="VHK28_07345"
     CDS             complement(4390..>4785)
                     /locus_tag="VHK28_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142069.1"
                     /translation="AAEGRRLASQGVLVLYDRYPLPTARLGGRTVDGPRIRQLPAAER
                     SGLLQRLARREEAMYRGIALPDALLVLRIAADVALARKASRDPAGAARKADALDAMDR
                     EATRVIDVDAGQPLDEVVRDVKRNIWELL"
BASE COUNT          779 a         1719 c         1637 g          650 t
ORIGIN      
        1 agcacgatgc ccaccagcaa tggacgctgc agggaacgcc gatcggtgat ggtggcgaac
       61 cagtagatgg ccagcattga gaagagcacg agggtgatgg tgtacggagc gggcccgccc
      121 agcgtgccga ccgtgcgctc gcccgcgcgt cccagccagc cctcgggaag cagcgagggc
      181 gcgacccagc tcatgacgcc gatgacgcac tggatgacca cggtcaccgc gccgatcgcg
      241 agaaggtgtg acagctcacg cctgcccggg gcgagcgccc ggataagcca gaagagcagg
      301 atgggcatgg ccagcttgtc gtagaaggtc accataccgc gcagcgggct ggggctgagg
      361 tagagcacat tgaagacgcc ggccgccagg aagagcagga gcatcgcgtc gaccaggtgg
      421 aaccggaagg agcggttgcg cagcccgagg acgtgcagca gcagcaccag caccagcagc
      481 gccggcagtc ccaggcgatg gacgccccag aacacgaggc tgggctgctc cgagccgagg
      541 cggacgatga agggcatcac cagcagccag atcaggatgc cggcgaacgc gtagcgaatg
      601 accagcagga tggccggcac cgcggcggcc gccaccagcg ccatctccca gcgaccgccg
      661 gcgaccaggt agcccagacc gaccgcgaag acagacgccg ccgcgaccgc ggccagcgcg
      721 agccgccggt cgcgcgggag cccgcccggt tcggccgagg gctgcatggc gcgtcccggc
      781 agcgggaggg cacgatcagt cgccaccaga ttcgcctcca gctgtgtgat ccagacccgg
      841 tgctatagtc ccacggcaat cggtgcagtc acgccccagc gcgtcaccga ggacggggag
      901 ctcatgatca tcagccatac gcatcggtat gtgttcgtgg aactgccgcg gaccggctcg
      961 acggcgatcc gcctggagct ccgcgaactg tacgacgggc agccgatcct ccacaaacac
     1021 gccacctacg acgagttcct gcgccaggcc tcccctgagg agcgcacgta cttcagcttc
     1081 gccgccatcc ggaacccgct ggacgacgcg gtgagccgat acttcaagct gcggaccgat
     1141 cacaaggggc gctactctgg caccggcaac aagaagcggt tcaagctgct gaagcggctc
     1201 caggacgatc gtatgtatcg gttcatcgag aggaccgatg cggacttccc caccttcttc
     1261 cggcgcttct acgtcctgcc ccacgacacc tgggccagcg tcacccacca ccgcctggac
     1321 ttcgtgatgc gcttcgagag cctggcgtcc gacttcgagg aggcgctgcg gcgcatcggc
     1381 atccagccga agcgcccact cccgatggtg aattcgaccg ccagccgtcg ccgctcgttc
     1441 gacacctact acacccccga cctctatccc cgggcgcggc gcgtcttcgg accgtacatg
     1501 gagcgctggg gctacacctt cccggacggc tggcgcgtcg ggccgcccac cacgctgcac
     1561 cgcgcccagt atcgagccgt caacaccctg gccaggatct actggcgctt cctgcgcccg
     1621 tacgcctgat cctccccggt ggagccgcga tacgtcctgt tggcctcgaa cgcatactcg
     1681 gggtccacgc tcctgtcctt cctgttgggc gcccacccca acatcgccac cgtcagcgac
     1741 gtctccggtg cccgcgggca gcaccggatg gacaccttcc gctgctcctg cggccgactc
     1801 atggcggagg atccattctg gctggaggtc cgcgatcgca tgcgagccgc cgggtggacc
     1861 gatttcgacc ttgccaactt ccggctcggc ttcgattacg gccggtcccc gcgattcaac
     1921 cgcctgcgca ccgggaccat gcgctggcac tccctcgagg cgctccggga cgcggccttt
     1981 cgcgcctggc cgggcgacga gtccaggatg cgatccatcg gcgcccggac ggcctccctg
     2041 gcgagggtgg tgaccgagct gtccggcgcg aacgtgttcg tggacgcctc gaaggagcgc
     2101 ttgcgggcgg cgtacctgca gcgttatctg gcgctcgacc tcagggtcat ccacctgctg
     2161 cgcgacgtcc gcgcggtggc cgcgtcggcg cgccggcacg gccggagcgg cacgagcacg
     2221 gtcgccgagg ccgccagccg ctgggcgggc accaaccagg ccattacccg cagccttggc
     2281 gccgtgcccg gcgcccagcg cacggttgtt cgctacgagg acctctgcgc ccgccccgac
     2341 caggagctcg cacgcctgta ccggttctgc ggcgtcgacc cggaccgagc gccgccgctg
     2401 gagcacgctc ccgttcagca cctgctgggc aaccgcgttc ggctcgcgaa cctcaccgac
     2461 atccgcctcg accggcgatg gcaggaggag ctgtcccccc aggagcaggc tgacgcgctt
     2521 cgggcggctg gcgccacgta ccctcgtttc tacgggtccg gacctcaagc atcgaaggtg
     2581 tagccggcca ggaatgggaa ctcggcgatg aggccgtcca cccgctcacg catctcgggc
     2641 ggccactcga tctccgagcg gcttcgcgag ggacgggcgt agagcacccc tgcgcaggcg
     2701 tcgaggtact cggacgtggc aaccacctcg agcaggtcgc acgcctcggc caggcgcgcg
     2761 cgtggatcgg agatcaggtc ctcgtgccgc atggtcagca gacgatcggg accgatggcc
     2821 tggcggaggt cgaacaggcc ccggcaggtc gcgaagtagc gctccacggc attcggcagc
     2881 gtgcggccgc cgcgaatgac catggtgctg atgttgtcga acgggttgcg gacgacgtgg
     2941 atgaatcgga gctgcagggg ccgcatccgt cgctccagcc gccgcagcaa agccgggtcg
     3001 gacgagagac ggcgcaccgt ccagcccgcc cggctgtcgc ccaccacccg cagctcgcgg
     3061 aagcggccct gccactggcc ctcgacgtgg tagctgtagg tcccgccgcg cccgagcttg
     3121 cgccgctggt gcgcggcctg gtggcgtgcc gtccgcagcg agaggaacag gacctggtcg
     3181 cgcgtgaatc ccgccgagac gtaccgcagc gcgtccagct cgtcggagac caccatctcg
     3241 gggtgggcat ccagcagtgc gccgacgatg ctgtggccgc tgcgcggatg accgatgaac
     3301 aggcagtacg tccggacctg gcgcaggctg gccgcctcgc gcctggcgcc gactgccgca
     3361 accgcgaagc gccaggccag gtgcaacggc gtgccccgca ccgcgccgcg cagtgattcc
     3421 agccgcgaac ccatcacgcc gccgggtagc cgaaacgcgc cagctcggca cgcagggtcg
     3481 tggcgaacca agctcgccag ggacgccaca cccgcgagcg ccagcgcgtg gcggcggacg
     3541 agtcgatgcc ctgcccggca tgacgcacgg ccgcgaagct cgagccgacc acctccgccc
     3601 cgctcagcag tgcggggtcg tagggaaggt ccaggaaggc gaggacccgt cgcagctcat
     3661 gttccgggtc cgtcacgagg tcctcgaagc ggaccagcag gtatcggtcc ccgaacagcg
     3721 cgcggtactg ctcgtccagg cgaaccgcgt cgagccatgc gcggcgggtg cggacggcct
     3781 cgtagggtgc caagagcggg tcgatcagcg caccggggat ccacccgaca cgggctttcg
     3841 ggccccatcg gccctgccgc aggcggacca gctgtgatgc gaagatgccg cgcgggtcgc
     3901 gcatggtatg gatgaacctg gcacgcggga accattcgct caggcggggc accgcgtaga
     3961 gatgcgccgg ggtcttctcg ccgacgattc cgtcctcagc gcgttcgtcg gcgcacgccc
     4021 gttcggcgta cagtccgatc aaggcccgga atacctcctt cggtgacagg tcggtgccgg
     4081 acaggcgctc gatgagctcg tccggcccga cgttgcgctc gagccagcgc catccgttgg
     4141 gaccggccag gtccgaggcg aaccggcgca gcgccgccga tcgctcgacc tcgggcgcgc
     4201 tgagcacggc ggccagccgt cgctcggcgc gaaacccgtt ccggccggcg aggaagtgcg
     4261 tctcggaggc caggcagatc cgcgaggagc ggttgagcag gtggcgcagc aaagtggacc
     4321 cggtgcgcgc gcagcccacg atgaacagcg gccccggtcg ctgcgccact gcctggggtg
     4381 cccggttgct cacagcagtt cccagatgtt ccgtttgacg tcgcgcacga cttcatcaag
     4441 tggctgcccg gcatccacgt cgatgacccg ggttgcctcg cggtccatcg catccagtgc
     4501 gtccgccttg cgcgccgcgc ccgccggatc gcggctggcc ttgcgggcga gtgccacgtc
     4561 ggccgcgatg cgcaggacga gcagggcatc gggcagtgcg atgcctcggt acatcgcctc
     4621 ctcgcgcctg gccaggcgct gcagcagccc ggaacgctcg gccgccggca gctggcggat
     4681 gcgagggccg tccacggtcc gtccgccgag acgggcggtc ggcaacgggt agcggtcata
     4741 cagcaccagc actccctggc tcgccaggcg gcgcccctcc gcggc
//
LOCUS       DATACA010000356         5992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_285896, whole genome shotgun sequence.
ACCESSION   DATACA010000356 DATACA010000000
VERSION     DATACA010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285896"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1443)
                     /locus_tag="VHK28_07350"
     CDS             complement(211..1443)
                     /locus_tag="VHK28_07350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142070.1"
                     /translation="MSEAPPHRFLSPAILGIAAGMMLVPLNSTMLAVALPSVLEEFDV
                     SAGTVATLVTVYLGAMVLALPPAGALGDRLGHRRTFLAGVAGFAAASLLAAVAGAFWV
                     LILARVLQAASGSLISTSAAALVRAAAPENQRGAAFGTFDMLVTVSAAVGPFVGGVIV
                     GALDWRWMFLLAVPVGAFAALSVLAWHPPGTDAAQPRPGRFVPINLRLFTRREFSAAV
                     AGILGATVILHGSFILVPLMVEQLMGATATTAGLVLLGISGVSAVAAPLGGRASDRIG
                     RRAPVMLGSLVTAVGLGLLAWYVVAVPLSLASAIPLALLLGVVGAGFGLAGSARQAAA
                     LEAVPAHEVGVAAATYYTGRYLGGVIGASLAGAVLTDAVVPGSVSLGFVILAVVAAAV
                     ALVSAGLPGPREVPATPA"
     gene            complement(1440..3041)
                     /locus_tag="VHK28_07355"
     CDS             complement(1440..3041)
                     /locus_tag="VHK28_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX2142071.1"
                     /translation="MTPILPPGPPDFPFEWEHESDAELSWEWDNMHGPNPLAPMAADY
                     MQVIGQGFGYRFERLDAPMQVIFRTWHGYLYTTARLHVPSGGEEAFGQRMLERRRGVI
                     PTTSDYWNRKAIPELRAAYASIDAIQVEGADPETLAADWERAWRLLERAWSIHFFVIT
                     GAYQVLDDLADRYEALVPGASAGEALRLVQGRLGDLQDVGDALERLVDLARQDPIAGE
                     ALRAGAYDDLPTDGPFHDELARFLERHGHLGQGWDDLQLASWSEDPTPLYSDLAKRLD
                     QPGERAEDRRRRLQDDSDRLLEEVRARLASKPDELRDFESLLGHAREIGHLTETHNYW
                     IDRMAQATLRRLAMRVGRRLVGDGVIGAVEDVFYLHRSEIPELIRAPRGMTGTLAERK
                     AEHERQRTMTPPPWIGQPPEGGGGRFDTPERVEHQAQEVRGAGASPGIARGPARVALG
                     QDDFDRIQPGDIIVCPSSNPSWVPLFSIAAGLVTDAGGVLSHAAVVAREFGLPAVVGT
                     RDGTSRIPDGRLIEIDGSAGTVRIL"
     gene            complement(3041..3940)
                     /locus_tag="VHK28_07360"
     CDS             complement(3041..3940)
                     /locus_tag="VHK28_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX2142072.1"
                     /translation="MLQPSDARDRLIEWLGATDAPASVLDELLGGKGASLERLLRMGA
                     PTPPGFCLTTTAFRHQLDASAETDAVARAIAALPDEDARSTLVRLLTDAPLAPALASA
                     LEDCVRRLASRNGGEEARFAVRSSAVGEDSRLASFAGVHETELGLTDAQVPGAIRRCW
                     ASLWSERAIQYRASRELPMDGAMAVVVQVLVPAEAAAVVFTRHPITQRDDQLLVNSVS
                     GLGEALVSGIITPDELVLDKATLRAVSIVPANGQPVLADGELRELGTLALELEAAFRC
                     PIDIEAALAGGKWHVLQARPITA"
     gene            complement(4003..4881)
                     /gene="sigJ"
                     /locus_tag="VHK28_07365"
     CDS             complement(4003..4881)
                     /gene="sigJ"
                     /locus_tag="VHK28_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX2142073.1"
                     /translation="MNPPDQRAAQFEEHRGHLKAVAYRMLGSLSEAEDAVQDAWLRYS
                     RSDTSDVENLGGWLTTVVARICLNLLRARRSRRELPSSLRLPDPVVTRAEETDPAEQA
                     LVADAVGMAMLVVLETLSPAERVAFVLHDVFGVPFDQVAPIVGRTPTAARQLASRARR
                     RVHQSPAQPDADLRQQRAVVSAFQAAARSGDFQALLAVLDPDVVVRADFGARRATQEN
                     RGAVSVAENAMSFAPLAGFARPALVNGTAGLVIVRDGRPLAVLGFTVRGGKIVELDIL
                     GDPARLRHLDVSILDD"
     gene            4960..5421
                     /locus_tag="VHK28_07370"
     CDS             4960..5421
                     /locus_tag="VHK28_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2142074.1"
                     /translation="MIHAARPVDRPPLFVRLFDPVARRLLKAGAPMGPNTLLTVRGRK
                     SGQPRTAAVALVEIGERRWVISAFGDTHWVRNLRDAGEGLLRVAGREEPVRAVELTHA
                     EAEAFFRDVLAPYVAGMGFPMRMVGRIFTRDVSADPEATAARRPVFELSRP"
     gene            5513..>5992
                     /locus_tag="VHK28_07375"
     CDS             5513..>5992
                     /locus_tag="VHK28_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX2142075.1"
                     /translation="MEGNGNVPAVRLGTEENPLIHPMTKERLVFLRRSADTGGELLEL
                     QLSLAPGGRIATTHVHPEQEERFEVGATPVMMRIGRVERMYQPGETVVVPPGVPHVWW
                     NPSDQEATTLVQFRPALDTETFFETFFGLAADGKANRQGVLNPLHMMVLGRVYRREVA
                     "
BASE COUNT          938 a         2113 c         2128 g          813 t
ORIGIN      
        1 cgcgcacgac ggccgcctga tgggcggccg tcgtgcgtgc cggccagtgg ctgcggggca
       61 gcgcgggggc tacgggatgg ctccaacggc caagcagcca gccagaatcg cggctcgctg
      121 gctgccacgc tcatgccgtg gctcgcgcgg ccatcgggga gcccgggtcc ttctcttctt
      181 ggccgttgga ctcacccggt ggctccggag tcaggcgggg gtcgctggaa cctcccgtgg
      241 accgggcagg ccggctgaca ccagcgccac ggccgccgcc acgaccgcca ggatcacgaa
      301 cccgagggag acggagccgg ggacgacggc atccgtcagg actgcgccgg ccaggctggc
      361 cccaatcacc ccgcccaggt agcgcccggt gtagtacgtg gcagcggcaa cgccaacctc
      421 gtgggctggt accgcctcga gggccgcggc ctgccgcgcg gatccggcca gcccgaaccc
      481 ggcgcccacc acgcccagca gcagcgccag cgggatcgcc gatgcgagcg acagcggaac
      541 cgccaccacg taccaggcca gcagccccag cccgacagcc gtgaccaacg aaccgagcat
      601 cactggcgcg cggcgcccga tgcggtccga cgcccgacca ccgagcggtg ccgccaccgc
      661 ggagacacct gagatcccaa gcagcaccag gccggcggtg gtggcggtcg cgcccatcag
      721 ctgctcgacc atgagcggca ccaggatgaa gctgccgtgc aggatgacgg tcgcccccag
      781 gatgccggca acggcggccg agaactcgcg ccgcgtgaaa agtcggaggt tgatgggaac
      841 gaagcggccg ggtcggggct gggcggcatc ggtccctggc ggatgccagg ccagcacgct
      901 gagggcggcg aacgccccga ccggcacagc cagcaggaac atccatcgcc agtccagcgc
      961 gccgacgatc acgccgccga cgaatggacc caccgccgcg ctgaccgtga ccagcatgtc
     1021 gaacgtgccg aacgcggcgc cgcgctggtt ctcgggtgcc gcggcccgca ccagcgcagc
     1081 ggcactggtc gagatcagcg agccgctggc cgcctggagg acgcgtgcca ggattagcac
     1141 ccagaacgcg ccggccacgg cggccagcag cgaggcagcc gcgaatcccg ccacccctgc
     1201 caggaaggtg cggcggtgcc ccaggcggtc gcccagcgcc ccggccggcg gcagggccag
     1261 caccatggcg ccgaggtaca cggtgaccag cgtggccacc gtgccggcgc tcacgtcgaa
     1321 ctcctcgagg acgctgggca gcgccacggc cagcatggtc gagttgagcg gcaccagcat
     1381 catgccggcg gcgattccaa ggatggccgg gctcaggaac cgatgcggtg gcgcctcgct
     1441 cacaggatgc gaacggtgcc cgccgaaccg tcgatctcga tcaggcgtcc gtccgggatg
     1501 cgcgacgtgc cgtcgcgggt gccgaccaca gcggggagtc cgaactctcg cgccacgaca
     1561 gcggcgtgcg agaggacgcc gccggcatcg gtcaccaggc ctgcggcgat cgaaaagagc
     1621 gggacccagg acgggttgga cgacgggcag acgatgatgt cgccgggctg gatgcggtcg
     1681 aaatcgtctt ggccgagtgc cacgcgggcc ggaccgcgcg cgatccccgg cgaggcaccg
     1741 gcaccgcgca cctcctgggc ctggtgctcc acgcgttccg gcgtgtcgaa gcgcccgccg
     1801 ccgccctccg gtggctggcc gatccagggt gggggagtca tggtccgctg gcgctcgtgc
     1861 tccgccttgc gctcggcgag ggtgccagtc atgccgcgcg gcgcgcggat cagctccggg
     1921 atctcggagc ggtgcaggta gaagacgtcc tccaccgcac cgatgacgcc gtcacccacc
     1981 aggcgccgcc cgacgcgcat cgcgagccgg cgcagagtcg cctgcgccat gcggtcgatc
     2041 cagtagttgt gcgtctcggt gaggtggccg atctcgcggg cgtgacccag gagcgactcg
     2101 aagtcgcgca gctcgtcggg cttactggcc aggcgggctc gcacctcctc caacagccga
     2161 tcgctgtcgt cctgcagccg ccgcctgcga tcctcggcgc gctcacctgg ctggtcgagc
     2221 cgcttggcca ggtcgctgta cagcggcgtc ggatcctcgc tccacgatgc gagctggagg
     2281 tcgtcccagc cctggcccag gtggccgtgc cgctccagga aacgagccag ctcatcgtgg
     2341 aagggaccgt ctgtgggcag atcgtcgtag gcgccggcgc gcagcgcctc cccggcgatc
     2401 gggtcctggc gggcgagatc gaccagccgc tccagcgcgt cacccacgtc ctggaggtcg
     2461 cccagccggc cctgcaccag gcgcagcgcc tcgccggccg aggcgccggg gaccagggct
     2521 tcgtaccggt ccgccagatc gtccagcacc tggtaggcgc cggtgatcac gaagaagtgg
     2581 atgctccacg cgcgctccag gagccgccag gcgcgctccc agtcagcagc cagggtttcg
     2641 ggatcagctc cctcgacctg gatggcgtcg atagacgcgt atgccgcacg cagctccggg
     2701 attgccttcc ggttccagta gtccgaggtc gtcgggatca cgccgcgccg gcgctcgagc
     2761 atgcgctgcc cgaatgcctc ctccccgcca ctcggaacgt ggaggcgggc cgtggtgtac
     2821 aggtagccgt gccaggttcg gaagatgacc tgcatgggcg cgtccagccg ctcgaagcgg
     2881 tacccgaacc cctgcccgat cacctgcatg tagtcggcgg ccatgggcgc cagcgggttc
     2941 gggccgtgca tgttgtccca ctcccaggag agctcggcgt ccgactcgtg ctcccactcg
     3001 aaggggaagt ccggcggtcc gggcggcagg atgggcgtca tcaggcagtg atgggtcgtg
     3061 cctggaggac atgccacttc ccgccggcca gcgcggcctc gatgtcgatg gggcaccgaa
     3121 acgccgcctc cagctcgagg gcaagagttc ccagttctcg aagctccccg tcggccagca
     3181 ctggctggcc gttggcgggc acgatcgaca cggcgcgcag agtggccttg tcaagcacca
     3241 gctcgtcggg cgtgatgatg ccggacacca gtgcctcgcc cagccccgac accgagttga
     3301 ccagcagctg gtcgtcgcgc tgggtgatcg ggtgccgcgt gaagaccacc gccgcggctt
     3361 cggccggcac gagcacctgc acgacgacgg ccatggcccc gtccatgggc agctcgcggc
     3421 tggcgcggta ctggatggcc cgctcgctcc acagtgatgc ccagcagcgg cgaatcgcac
     3481 cgggcacctg tgcgtcggtc aatccgagct cggtctcgtg cacgccggcg aaggacgcca
     3541 ggcgggagtc ctcgcccacc gccgaggagc gcaccgcgaa gcgcgcctct tcgccgccgt
     3601 tcctggacgc caggcggcgc acgcaatcct ccagggcgga ggccagggcg ggggccagcg
     3661 gcgcatcggt cagcagtcgc accagggtcg agcgggcgtc ctcgtccggg agcgcggcga
     3721 tggcgcgcgc gaccgcatcg gtctccgctg aagcgtcgag ctgatgacga aaggccgtgg
     3781 tggtcaggca gaacccgggc ggtgtgggtg cccccatgcg aagcaggcgc tcgaggctgg
     3841 ctcccttccc acccagcaac tcatccaaga ccgatgctgg cgcatccgtg gcgcccagcc
     3901 actcgatgag acggtcgcgt gcgtcgctcg gttgaagcat ctgccaccga tcagcggaag
     3961 gatcgacgca gtgtatatgc cgtcggtagc gggaggggct gctcagtcgt cgaggatcga
     4021 gacgtccagg tgccgcaggc gagccggatc gcccaggatg tcgagctcca cgatcttgcc
     4081 gccgcggacg gtgaagccga ggaccgccaa tggccgcccg tcgcggacga tgaccagtcc
     4141 cgcggtcccg ttcaccaggg ccgggcgagc gaaacctgcc agcggcgcga agctcatggc
     4201 gttttcggcc accgacaccg ccccgcggtt ctcctgggtg gcgcgtcgtg ctccgaagtc
     4261 ggcacgaacc accacgtccg gatccaagac cgccagcagg gcctggaagt cgcccgaccg
     4321 ggccgcggcc tggaatgcgg atacgacggc gcgctgctgc cgaaggtccg catccggctg
     4381 ggccggggac tgatgaacgc ggcggcgtgc gcggctggcc agctggcgcg cggcggtcgg
     4441 cgttcgcccg acgatcgggg cgacctggtc gaacggaacc ccgaacacgt cgtgcagcac
     4501 gaaggcgacg cgctcggcgg gcgacagcgt ctcgagcacc accagcatgg ccatgcccac
     4561 ggcatcggca acgagcgcct gctcggccgg atcggtctcc tcggcgcgcg tgacgaccgg
     4621 gtccggcaac cgcagggacg atggcagctc acgcctcgat cgccgggcgc ggagcaggtt
     4681 caggcagatg cgggcgacca ccgtcgtcag ccagccaccc aggttctcga cgtcgctcgt
     4741 gtcggacctg ctgtagcgca gccacgcgtc ctggacggcg tcctccgcct cgctcagcga
     4801 gccgagcatc cggtaggcga cggccttgag atggccccga tgctcctcga actgcgccgc
     4861 ccgctggtct ggtgggttca tccggtcacg ttcctcgttc gcattgtgtc agccagatga
     4921 accgatgcac cccacgaatg tgaccaggag gcttccgcca tgatccacgc cgcccggccc
     4981 gtcgaccggc caccgctgtt cgtccgcctc ttcgatccgg tggccaggcg actgctgaag
     5041 gccggggcgc cgatgggccc gaacacgttg ctcaccgtgc gtggcaggaa gagtggacag
     5101 ccgcgtaccg cagccgtcgc gctggtcgag atcggggagc ggcgctgggt gatcagcgcg
     5161 ttcggtgaca cgcactgggt gcgcaacctc cgcgacgctg gagagggact gctgcgggtg
     5221 gctggccgcg aggagccggt tcgcgccgtg gagctgaccc atgccgaggc cgaggccttc
     5281 ttccgtgacg tcctggcgcc ctacgtggcc gggatgggat tccccatgag aatggtggga
     5341 cgcatcttca cccgcgacgt ctcggccgat ccagaagcca ccgctgcgcg ccgcccggtc
     5401 ttcgagctgt cccgacctta agcggcagat ttgcttgacc gcggatgttc cctaggtgca
     5461 cgcttggcgg cgccgtgcgc cgagacacgg gcacaactga cggaggtccc gagtggaagg
     5521 caacggtaat gtccctgcgg tccgccttgg caccgaggag aacccgctca tccatccgat
     5581 gaccaaagag cggctcgtct tcctcaggcg gtcagccgat accgggggcg agttgctcga
     5641 gttgcagctc tccctggcgc cgggcggaag gatcgccacc acgcacgtcc atccagagca
     5701 ggaagagcgc ttcgaggttg gtgcgacgcc ggtcatgatg cgcatcggac gggtggagcg
     5761 catgtaccag cccggtgaga cagtggtcgt gccgccgggg gtgccgcacg tgtggtggaa
     5821 cccgagcgac caggaagcga cgaccctggt ccagttccgg cccgcgctcg acacggaaac
     5881 cttcttcgag accttcttcg gcttggccgc agacggcaag gccaacaggc agggggtgct
     5941 gaacccgctg cacatgatgg tcctgggccg tgtgtaccgc cgtgaggtcg ca
//
LOCUS       DATACA010000357         3463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_365996, whole genome shotgun sequence.
ACCESSION   DATACA010000357 DATACA010000000
VERSION     DATACA010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365996"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VHK28_07380"
     CDS             complement(<1..833)
                     /locus_tag="VHK28_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX2142076.1"
                     /translation="MTDSPARVRQVDIPEALRLAEAGYRVVDVREPFEWQAGHVAGAL
                     HVPLGELQARFEAEVPDRSTPLLLYCRSGARSGRAADYLAQRGYSGVVNLAALIEGWP
                     RQGGAWEAPGQALTPAQQRRYGRQLLLPEIGPDGQRRLLDSKVLVLGAGGLGSPVALY
                     LGAAGVGTIGLVDDDSVDESNLQRQVLHGVDRIGQPKTASARTTLNGLNPDTNVVEHG
                     ERLTQENAERLIGGYGLVVDGSDNLETRYALNDAAVRLRKTVVHASAYRWEAQVTTLV
                     P"
     gene            complement(842..1246)
                     /locus_tag="VHK28_07385"
     CDS             complement(842..1246)
                     /locus_tag="VHK28_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2142077.1"
                     /translation="MDNLYRDFILEHYRNPHNKGVLDPHDLHFADSNPTCGDEMSMTL
                     LLDAEKLRVTDVAFDGRGCAISQASASILTDELRGMSVDQVRDMDPGTLLEALGVPIG
                     PARLKCALLSYKVAQGAVRGAEVPWPEEAEAL"
     gene            complement(1251..2516)
                     /locus_tag="VHK28_07390"
     CDS             complement(1251..2516)
                     /locus_tag="VHK28_07390"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX2142078.1"
                     /translation="MAVEPAAATVPGGFSGTALRADFPVFDQPTGTGKRLCFLDAAAS
                     APKPRAVIDAVADTYAHHYANVHRGIYELSEDATGRFEGARRKLARFINAPSEREVVF
                     VRNATEAINLVAYSWGRSNLAAGDRIVTTQLEHHANIVPWQQLAAATGAELDYVAITD
                     DGRLDMDDLRTKLARKPKLLAVAHVSNALGTINPLQEIIGLAHQAGALVLVDAAQSAP
                     HMRVDVQQLDCDFLALSGHKMLGPSGIGALWGRRALLDAMPPFMTGGSMIIRVSMEGA
                     EWNEVPAKFEAGTPAIAEAIGLGAAIDYLDGLGMDAVREHERWLFCQAWEALAGIPGV
                     RRLGPDDPEIHAGVISFLIDGVHPHDVATIFDHEGVAVRAGHHCAQPVMQRYDVPATT
                     RASFYVYNDADDVEALADAVRATQRIFGG"
     gene            complement(2527..3387)
                     /gene="sufC"
                     /locus_tag="VHK28_07395"
     CDS             complement(2527..3387)
                     /gene="sufC"
                     /locus_tag="VHK28_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX2142079.1"
                     /translation="MTDQLEIRGLEVSIAGKKILNGLDLTVKQGEVHALMGPNGSGKT
                     SLAYTLMGHPDYTVNAGTVTWRGENILAMTPDKRAQAVFFLAFQYPSAIPGVTVASFL
                     RNIYNAVHHPKQPAEVEAAGGVPKYSGIPLAKFRKLLEEKMALLKMDPGFSTRYVNEG
                     FSGGEKKRLEMLQMAVVSPKMAILDETDSGLDIDALRTVAEGLNAALNPEMGVLMITH
                     YQRMLNYVKPQFVHVLLDGRIVMSGGEELSHQLEANGYDWVREQVGLPEGTGDEAGAP
                     AAAGTVAEHV"
BASE COUNT          482 a         1204 c         1213 g          564 t
ORIGIN      
        1 aacgggacca gggtggtgac ctgcgcctcc cagcggtagg cggaggcgtg caccaccgtc
       61 ttgcgcagcc gcacggccgc gtcgttcagc gcgtagcgcg tctccaggtt gtcgctgccg
      121 tccacgacca gcccgtagcc gccgatcagc cgttcggcgt tctcctgcgt cagccgctcg
      181 ccgtgctcca cgacgttggt gtccgggttc agtccgttca gcgtggtcct ggcggacgcg
      241 gtcttgggct gaccgatgcg atccaccccg tgcagcacct gccgctgcag gttcgactcg
      301 tccacggagt cgtcatccac caggccgatg gtccccacgc cggcggctcc aaggtagagc
      361 gccaccggtg atcccagccc gccggcgccc aggaccagca ccttgctgtc cagcagccgg
      421 cgctggccgt caggcccgat ctccggcagt agcagctgcc gaccatacct gcgctgctgg
      481 gccggggtca gcgcctggcc gggcgcctcc caggcaccgc cctgccgcgg ccagccctcg
      541 atgagcgcgg cgaggttgac cacgccggag tacccgcgct gggcgaggta atcggccgcc
      601 cgtcccgacc gagcacccga gcggcagtag agcaggagcg gggtggagcg gtccggcacc
      661 tcggcctcga agcgcgcctg cagctcaccc aacgggacgt gcagcgcgcc ggcgacatgg
      721 ccggcctgcc actcgaaggg ctcccgcacg tcgaccacgc ggtagccggc ctcggccagc
      781 ctcagcgcct cgggaatgtc gacctgtcga acgcgcgccg gcgaatcggt catgcggggg
      841 ctcagagcgc ctccgcttcc tcgggccagg ggacctcggc gccgcgcacg gcgccctgcg
      901 ccaccttgta ggagaggagc gcgcacttga gccgggccgg gccgatcggc acgccgaggg
      961 cctccagcag cgtgccggga tccatgtcgc ggacctggtc cacgctcatg ccacgcagct
     1021 catcggtcag gattgaggct gatgcctggc tgatggcgca gccgcggccg tcgaaggcga
     1081 catcggtcac ccgcagcttc tccgcgtcga gcagcagcgt catgctcatc tcgtcaccac
     1141 aggtggggtt ggagtcggcg aagtgcaggt cgtgcgggtc gagcacgccc ttgttgtgcg
     1201 ggttgcggta gtgctcgagg atgaagtcgc ggtagaggtt gtccatctgg ctagccgccg
     1261 aagatgcgct gggtcgcccg caccgcgtcc gccagggcct cgacgtcgtc ggcgtcgttg
     1321 tagacgtaga agctggcccg ggtggtggcc ggcacgtcgt agcgctgcat gaccggctgc
     1381 gcgcagtgat ggccggcacg gaccgccacg ccctcgtgat cgaagatggt ggccacgtcg
     1441 tgcgggtgca cgccgtcgat caggaagctg atcacgccgg cgtggatctc cgggtcgtcc
     1501 gggcccaggc gccgcactcc ggggatcccg gccagcgcct cccaggcctg gcagaacagc
     1561 cagcgctcgt gctcacgcac cgcgtccatg ccgagcccgt ccaggtagtc gatggcggcc
     1621 cccagaccga tggcctccgc gatggccggc gtgccggcct cgaacttggc tggcacctcg
     1681 ttccactccg cgccctccat ggagacgcgg atgatcatgc tgccgccggt catgaagggc
     1741 ggcatggcat ccagcagggc acgccgtccc cacagggcac cgatgcctga cgggccgagc
     1801 atcttgtgcc cggacagcgc caggaagtca cagtccagct gctgtacgtc gacgcgcatg
     1861 tgcggcgcgg actgagccgc gtccaccagc accagggcgc cggcctggtg cgccaggccg
     1921 atgatctcct gcagcgggtt gatggtgccc agcgcgttgc tgacgtgggc caccgccagc
     1981 agcttcggct tgcgcgcgag cttggtgcgc aggtcgtcca tgtccaggcg gccgtcgtcg
     2041 gtgatggcca cgtagtcgag ctcggccccg gtagccgcgg ccagctgctg ccagggaacg
     2101 atgttggcgt ggtgctccag ctgcgtggtc acgatccgat cgccggcggc caggttggag
     2161 cggccccacg agtaggcgac caggttgatg gcctcggtgg cgttgcggac gaacaccacc
     2221 tcgcgctcgg acggggcgtt gatgaagcgt gcgagcttgc gtcgtgcccc ttcgaagcgg
     2281 ccggtggcat cctccgacag ctcgtagatg ccgcggtgca cgttggcgta gtggtgggcg
     2341 taggtatcgg ccaccgcgtc gatcaccgcg cgcggcttcg gcgcgctggc cgcggcgtcc
     2401 aggaagcaga gccgcttacc ggtgccggtg ggctggtcga agaccgggaa gtcggcccgc
     2461 agcgcagtgc cggagaagcc tccggggacc gttgcggccg cgggctcgac tgccatccgc
     2521 cctagtttag acgtgctccg ccacggtccc ggccgccgcc ggtgcgccgg cctcgtcgcc
     2581 ggttccctcc ggcaggccga cctgctcgcg aacccagtcg tagccgttgg cctccagctg
     2641 gtgcgacagc tcctcgccgc cggacatcac gatccggccg tccagcagca cgtgcacgaa
     2701 ctgcggcttc acgtagttga gcatgcgctg gtagtgggtg atcatcagca cgcccatctc
     2761 cgggttgagc gccgcgttca ggccctcagc caccgtgcgc agcgcgtcga tgtccaggcc
     2821 cgaatcggtc tcgtccagga tggccatctt ggggctcacc accgccatct ggagcatctc
     2881 caggcgcttc ttctcgccgc ccgagaaccc ctcgttgacg tagcgcgtcg agaagcccgg
     2941 atccatcttg agcagcgcca tcttctcctc gagcagcttg cggaacttcg ccagcgggat
     3001 gcccgagtac ttgggcacgc cgccggcggc ctcgacctcg gccggctgct tcgggtgatg
     3061 gaccgcgttg tagatgttgc gcaggaagct ggcaaccgtc acgccgggaa tggcgcttgg
     3121 gtactggaag gcgaggaaga agacggcctg ggcccgcttg tcgggcgtca tggccaggat
     3181 gttctcgcca cgccaggtca cggtgccggc gttgacggtg taatccggat ggcccatcag
     3241 cgtgtaggcc agcgacgtct tgcccgaacc gttggggccc atgagggcgt gcacctcgcc
     3301 ctgcttcacg gtcaggtcga ggccgttgag gatcttcttg cccgcgatgc tcacttcgag
     3361 gccgcggatc tccagctgat cggtcatgcg cgtgtgcttg tgctcctgct aggtggtcgc
     3421 cgaggtcggg cccatcggct cgaccggcgt cttgggattc ggg
//
LOCUS       DATACA010000358         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1973043, whole genome shotgun sequence.
ACCESSION   DATACA010000358 DATACA010000000
VERSION     DATACA010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973043"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..730)
                     /locus_tag="VHK28_07400"
     CDS             complement(11..730)
                     /locus_tag="VHK28_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142080.1"
                     /translation="MFRPTIALLALLLLATACGGGTTPSGAASAADPSQTSAASSDTE
                     PSEVAEATPEPSAEIADACDLVPDELAGFTLIENDQNGVVNTPETLEEEAAEADAGTA
                     SILTSRAAFWNAMGVMENCQEGFETDDAAVIVIATTFMNEQGRTQYYANEWGEGSGCE
                     PLDAEFPAAPDATLYTCVGARPAAYVAAVVPDGTTTGWEIISRADYGSTTVTDEVTVA
                     ALNDAVAQAGAVVAELPGFDG"
     gene            complement(742..2877)
                     /locus_tag="VHK28_07405"
     CDS             complement(742..2877)
                     /locus_tag="VHK28_07405"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEX2142081.1"
                     /translation="MPTPVRVWDASRVVLPLALVVIACLALPPVPARAATPTPDALRE
                     FLEGYGLICLDVPSEESGRLTVWCDPPGYSGEFVDWRGVYGTNQGELDAGMNFEISSP
                     GLDAPDGAHLEFLVRGAMLQCEDDGEVMEFVTANAMAPPFGQDATYVPVEELQGCAVD
                     LRVAASGSAEDPVNQTFLWVGPPAPDQTPQPPVAGGTAVWLNDFLDLVGTRGLVCDDA
                     AANAATGGTEVRCDVPGSVGVHTGYRASYSLDASGQAVGYSLETATPGLDPPDGAHLQ
                     FLVDGATLMCEDDSADLGAFITDNATASPEAPSTSYETDTCVVFVTSMAGGQANELNQ
                     TFVSVGAPTLPTGEGEAPVEPAAPQEPGAEEPGVVTPPAGAPAAGDQPVAFDRSVPAP
                     SEISADPLVLLQSALLAVLVVFLMPFPGQLFNSTLETHEDEVRRWLRLDRLTRIGEGI
                     GAFWTSWPGVAAFTLLATLLYGFLDPTFGLSVDSAATFLGMLVGIIVVTVLAAAPMLL
                     LMLRHRERPQIKVVPVSLMVGVACVLVSRLTDFQPGYVYGLLIGLAFARELSAAEEGR
                     TTAIGAAGLFAVAMVAWLALGLLPDDGGFGLTVLRTALAALMVAGLEGMVFGLMPLRF
                     LPGASVMAWSRVAWGFLLGIGLFAFFHILINPASGYLSDSSRTPLLTTLALLIGFSAL
                     SVAFWAWFRFRNAPPEVPVDVDLPRPSVH"
     gene            complement(2982..>3736)
                     /locus_tag="VHK28_07410"
     CDS             complement(2982..>3736)
                     /locus_tag="VHK28_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2142082.1"
                     /translation="VIPEMELGAAELLWLGVRAEVEAVSRRALSRNDHRLAQVARHAA
                     ELVERSTAVTAAARGYGPRWQRRLGLLQQMAELEMARLTGSDDADSWAAVVTGWDLLG
                     IPYPAAYAQLRQASSLLRGRARTDAAIVLLEAHARATRLGAGGLQSAISALAKRSRVE
                     LPDETNAAADPATGPRPETAGALAALATLTDREREVLDLVAGGLSNRDIADRLFISER
                     TAAVHVSNVLGKLGVSRRTEAAAIALSGRPPS"
BASE COUNT          634 a         1296 c         1274 g          532 t
ORIGIN      
        1 gccgtcatcg ctagccgtca aagccgggaa gctcggctac cactgcaccg gcctgcgcca
       61 ccgcgtcgtt gagcgctgcc accgtgacct catcggtaac cgtcgtgctg ccgtagtccg
      121 cccgcgagat gatctcccag ccggtggtcg tgccgtcggg caccacggcg gccacgtacg
      181 ctgctggccg ggcgccgacg caggtgtaga gggtcgcgtc aggcgcagct gggaattcgg
      241 catccagcgg ctcgcagccc gagccctcac cccactcgtt ggcgtagtac tgggttcgcc
      301 cctgctcgtt catgaaggtt gtagcgatga cgatcaccgc cgcgtcatcg gtttcgaatc
      361 cttcctggca gttttccatg actcccatgg cgttccagaa ggcggcccgg ctggtcagga
      421 tgctggcggt cccggcatcg gcctccgcag cctcttcctc cagcgtttcg ggggtgttca
      481 ccaccccgtt ctggtcgttc tcgatgagcg tgaagcccgc gagctcgtcc ggcaccaggt
      541 cacaggcgtc tgcgatctcg gcactgggct caggcgttgc ctctgcgacc tcgctcggct
      601 ccgtatcaga gctcgcagcc gaagtctggc ttggatcggc tgccgaggcg gcgccgctgg
      661 gcgtggtgcc tccaccacag gcggtggcaa gcagcagcag ggcaagcagg gcaatggtcg
      721 gacgaaacat ggaaacctcc ctcaatggac cgatggtcgg ggcagatcga cgtcaaccgg
      781 cacctcagga ggggcgttcc ggaaccggaa ccatgcccag aaggcgaccg atagcgcgct
      841 gaatccgatg agcaacgcca gggtggtgag cagcggcgtg cggctggagt cggacaggta
      901 gcccgacgcg ggattgatca ggatgtggaa gaaggcaaag agaccgatgc ccagcaggaa
      961 accccacgcg acccggctcc aggccatcac ggacgcacct ggcaggaagc gcagcggcat
     1021 gagcccgaag accatgccct ccaggccggc caccatcagc gccgccaggg ccgtccgcag
     1081 caccgtgagc ccgaagccgc cgtcgtccgg cagcaaaccc agagcaagcc acgccaccat
     1141 ggccaccgcg aacagcccgg cggcaccgat ggcggtggtg cggccttcct ctgccgcgct
     1201 cagctcgcgc gcgaaagcaa ggccgatgag cagcccgtac acgtaccccg gctggaagtc
     1261 ggtcaggcgg gagacgagga cgcaggccac gcccaccatc agcgaaacgg gaaccacctt
     1321 gatctgcggt cgctcgcggt gccgcagcat gagcagcagc atcggcgccg cggccagcac
     1381 cgtcaccacg atgatcccga ccagcatccc gaggaaggtc gccgcggagt cgacgctcaa
     1441 gccgaaggtg ggatccaaga atccgtacag cagcgtggcc agcagggtga aggcggccac
     1501 ccctggccag gatgtccaga aggcccctat gccttcaccg atgcgcgtca ggcggtcgag
     1561 gcgcagccat cggcgcacct cgtcctcgtg cgtctccagc gtcgaattga acagctgtcc
     1621 gggaaacggc atcaggaaga cgaccaggac ggcgagcagc gcgctctgca gcaggaccaa
     1681 cggatccgcc gagatctcgg agggtgccgg gacggaccta tcgaacgcga ccggctgatc
     1741 cccggcggcg ggagccccgg cgggtggcgt gaccacgccc ggctcctcag cacccggttc
     1801 ctgcggggcg gcgggctcga cgggcgcctc cccctcaccg gtgggcagcg tcggcgcgcc
     1861 aacgctcacg aaggtctggt tcagctcgtt ggcctgtccc cccgccatgc tcgtgacgaa
     1921 caccacgcac gtatcggttt catacgaggt gctcggagcc tccgggctgg ccgtcgcgtt
     1981 atccgtgatg aacgcgccga ggtccgcgga gtcgtcctcg cacatgaggg tcgcgccgtc
     2041 caccaggaac tgaagatggg cgccatccgg cgggtccagt ccgggcgtgg cggtctcgag
     2101 cgagtagccg acggcctgcc ccgaggcgtc gagcgaatag gaggcgcggt atccggtgtg
     2161 cacgcccacg gagccgggga catcgcatcg cacctcggtc ccgccggtcg cggcgttcgc
     2221 cgcggcgtcg tcgcacacca gcccgcgcgt gccgaccaga tcgaggaagt cattcagcca
     2281 caccgcggtg ccgccggcaa ccggaggctg tggagtctgg tcgggtgccg gtggtcccac
     2341 ccacaggaac gtctggttga ccggatcctc tgccgagccg ctggcggcaa cccggagatc
     2401 gacggcgcag ccctgaagct cttccaccgg cacgtaggtg gcgtcctggc caaagggcgg
     2461 cgccatggcg ttcgccgtga caaactccat cacctcccca tcgtcctcgc actggagcat
     2521 cgcgcctcgc accaggaatt cgagatgcgc accgtcgggc gcgtcgagcc cgggagaact
     2581 gatctcgaag ttcatgccgg cgtcgagctc accctggttg gtcccgtaca cgccacgcca
     2641 gtcgacgaac tcgccgctgt agccgggagg gtcacaccag acggtcaggc ggcccgattc
     2701 ctcgctcggg acgtccaggc agatcaggcc atacccctcc aggaactcac gcagggcgtc
     2761 aggcgtcggg gtggcggcgc gggccggcac agggggcagg gcgaggcacg cgatcactac
     2821 gagcgcgagt ggcagcacaa ctcgggatgc gtcccagacg cggaccgggg tgggcacggc
     2881 gggcaggaac ctgaagatga gtacccgtca agtgtcgacc gtatcccgat gcgggggatc
     2941 gtcaggaatg actatttctc actcgccaga ccgcgagcgg gtcaggacgg tggtcggccg
     3001 ctcaacgcga tggctgctgc ctctgtgcgg cgggaaaccc ccagcttgcc cagcacgttc
     3061 gacacgtgga cggcagcagt ccgttcgctg atgaagaggc ggtcggcaat gtcacggttc
     3121 gacagtccgc ccgcgaccag gtcgaggacc tcgcgctctc ggtccgtcag cgtggccagc
     3181 gcagcgagtg cgccagcggt ttcgggacgc ggtccggtcg ccgggtccgc ggctgcgttc
     3241 gtctcgtcgg gcagctcgac cctggaccgc ttggcgagcg ccgagatggc cgattgcagc
     3301 ccgcccgcac ccagccgggt ggcgcgggca tgcgcctcga gcaggacgat ggccgcgtcc
     3361 gtccgcgctc ggccccgaag cagcgacgac gcctggcgca gctgcgcata ggcggctgga
     3421 tacgggatgc ccagcaggtc ccatccggta accaccgccg cccagctgtc cgcatcgtcg
     3481 ctaccggtga ggcgcgccat ctccagctcc gccatctgct gcaataggcc gagccggcgc
     3541 tgccagcgag ggccgtagcc gcgagcggca gcagtgaccg cggtcgatcg ctccaccagc
     3601 tcagcggcat gtcgagccac ctgcgcaagg cgatggtcat ttcgcgagag tgcgcgtcgc
     3661 gacaccgcct cgacctcggc tcggacgccg agccagagca gctcggcggc gcccagctcc
     3721 atttcgggga tgacgg
//
LOCUS       DATACA010000359         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_367448, whole genome shotgun sequence.
ACCESSION   DATACA010000359 DATACA010000000
VERSION     DATACA010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367448"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VHK28_07415"
     CDS             <1..273
                     /locus_tag="VHK28_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142083.1"
                     /translation="PDEPAITLLRTAGFSSAFAAIHGADPEWTYPSPMTPADVAVRPP
                     SPIDYVWVSGSAQVVDASTAVDRPSPHDPSLYPSDHRAVVADLEVR"
     gene            complement(344..643)
                     /locus_tag="VHK28_07420"
     CDS             complement(344..643)
                     /locus_tag="VHK28_07420"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX2142084.1"
                     /translation="MPYAPLLTEPEIAAEMERLPEWTREGEWISRTVRCASFREAIEL
                     VNRVADAAEVADHHPDITVVWRRVTFRLTSKASGGITARDVDMAQRIETAAAPRD"
     gene            complement(685..1266)
                     /gene="folE"
                     /locus_tag="VHK28_07425"
     CDS             complement(685..1266)
                     /gene="folE"
                     /locus_tag="VHK28_07425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2142085.1"
                     /translation="MDEPVPGGDLERAVRTLLSEIGEDPERQGLVATPSRVRRMYDEL
                     TDGYRTDPETLVNGACFDVEYDEMVVVRDIEFFSLCEHHLLPFHGHAHVGYLPRGKVI
                     GLSKIPRIVDMYAHRLQIQERMTQEIATFLMDRLDPKGVACVVEATHMCTVMRGVKKQ
                     EATMVTSSMLGTFRRDGRTRSEFLTLIGRSSGR"
     gene            1375..1950
                     /locus_tag="VHK28_07430"
     CDS             1375..1950
                     /locus_tag="VHK28_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142086.1"
                     /translation="MIVHPIPHRTGRLPLRGLPGISGDRLAHALGRHLDDGHHYFRKV
                     QAGGQPPIDAVLVGPGGTWTITHAHVAGRFRKRNGHWYRWNRSTESWIPWDATPLHQA
                     RLAGHRLELYLERATLPAMVHATLLAEHHAEIDWEADQAPGVYVYNELERLAARVSRD
                     EELTPAQIDRIVALLDPRQPLPRFAASESSG"
     gene            2009..4063
                     /locus_tag="VHK28_07435"
     CDS             2009..4063
                     /locus_tag="VHK28_07435"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEX2142087.1"
                     /translation="MPPERSPAAAATVAGAVIVLLLAAWLLPPLAVVVVWPLLFVLPG
                     WALVAWTRPRIGSTGRLGLAVVLSVAISTHLVYWTSLAAGYERWTVFAVAAVLAIPLP
                     IAAWLGGLGRAGVLGAQLTAAWHAIRRERVAFAFAALAAAFVGLVLDAGLWKPTETGI
                     AAGGSNWSDLGVHLSIAQSLNAANFPPEVPYFAGAPLVYHWFADFHAAIAARAAGMFA
                     VPAFTVSSAILAGALALLVHGLARRLLRGGAARRAALLAAVLVIFGGGLGWIRWVSDI
                     AQGFGDPIGLMVNNSYDNYWFDVHDQAPWPYFRIPSVMGTGLLVHRATTAGLPMLVGA
                     VLLLVAGLPTARRARAGYRDRPMLIGLAGLLGAMLAPFHFFFFPAFLLLAMLYVLIGG
                     RLLDIRAPENAFVFLLPYLLAVPFASAALLNARGSGSLKPVLGWESAPLADGPAGVLF
                     FYVTNLGLPLLLAVAALLTPRTPWRWFLGAWAAALFAIPNLVQVSDIAFDMNKYFQAM
                     WVAVALLAGWLMRRWAWPAIAVVLLVSMPSPLLVAGWTAFNREQVIDYWGLEAAEWIA
                     ENTPEDAVFATDGWLNSPTDAAGRKRLLTFAPYVANLGFEPEVRVRQVRDMYCAGDPA
                     LTVDLMRSLDAAYLLEAGRPSPCDNPTDFAAAPGLEKVYEGGPIRIWHRTDAAAGTPA
                     AP"
     gene            4101..5273
                     /locus_tag="VHK28_07440"
     CDS             4101..5273
                     /locus_tag="VHK28_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934734.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2142088.1"
                     /translation="MSTAQQDPMARASGAVVSLEHVTKRFGPPGTPAAVDDLSLTISA
                     GEIVVLVGPSGCGKTTTMKMINRLVEPTSGRVTIDGEDVMGLPPEELRRRIGYVIQQV
                     GLFPHLTVADNVAVVPRLLRWKPARIRERVEELLELVGLEPSVYAARYPTALSGGERQ
                     RIGVARALAADPPLMLMDEPFGAVDPIRRDRLQNEFLRLQERVRKTVVFVTHDVDEAI
                     KMADRIAIMQRGGIVAQYDTPEVVLTQPASEFVEHFVGADRGLKRLSLARVGDLPLLE
                     PMTVRVGEVAADARRRLEEAAVDYALLVDADRRPLGWIDPGDLAGDGEIGPEAATPGA
                     PTVQPETTLRDALSGMLASAVQFGVVVDEREAVLGLISVNAISETLRAATAGDGGG"
     gene            5270..5614
                     /locus_tag="VHK28_07445"
                     /pseudo
     CDS             5270..5614
                     /locus_tag="VHK28_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5669..5768)
                     /locus_tag="VHK28_07450"
                     /pseudo
     CDS             complement(5669..5768)
                     /locus_tag="VHK28_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222372.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          747 a         2085 c         2013 g          923 t
ORIGIN      
        1 ccggacgagc cggccatcac cctgctgcgc actgccggct tcagctcggc gttcgccgcc
       61 atccacggcg cggacccgga atggacctac ccctcaccga tgactcccgc ggacgtggcc
      121 gtccgcccgc cgtcacccat cgattacgtg tgggtgtcgg gcagcgccca ggtggtggat
      181 gccagcaccg ccgtcgatcg gccgtccccg cacgacccga gcctgtatcc gtccgaccac
      241 cgcgcggtgg tggccgacct ggaagtgcgg taggagccac tggctcggcc tcatgcggcg
      301 gccgtcgggc ggcggttgga attcgcctca cgaatgggtg gcgtcaatca cggggcgcgg
      361 cggcggtttc gatccgctgc gccatgtcca cgtctcgcgc ggttatcccg cccgaggcct
      421 tgctggtgag gcgaaaggtg acccgccgcc agacgacggt gatgtcgggg tggtgatcgg
      481 cgacctcggc ggcatcggcc acccggttga ccagctcgat cgcctcccgg aatgaggcgc
      541 agcgcaccgt acggctgatc cattcgccct cgcgggtcca ctccggcagg cgctccattt
      601 ccgcggcgat ctccggctcg gtcaggaggg gcgcgtacgg catggaccga tcatatccgc
      661 gcaacagcgc gcctgagcct gcggctagcg gcctgacgag cgaccgatca gggtcaggaa
      721 ctcggagcgc gtccggccat cacgacggaa ggtgccgagc atggagctgg tgaccatggt
      781 ggcctcctgc ttcttcaccc cgcgcatgac ggtgcacatg tgggtggcct ccaccacgca
      841 ggccacgccc ttcggatcca ggcggtccat caggaaggtg gcgatctcct gggtcatgcg
      901 ctcctggatc tgcagccgat gggcgtacat gtcgacgatt cgcgggatct tcgaaagacc
      961 gatgaccttg ccgcgcggca ggtagcccac gtgagcgtgc ccgtggaacg gcagcaggtg
     1021 gtgctcgcac aggctgaaga actcgatgtc gcggaccacg accatctcgt cgtactcgac
     1081 gtcgaagcac gctccgttga ccagggtttc cggatccgtg cggtagccat cggtcagctc
     1141 gtcgtacatg cgccgcacgc gcgacggcgt ggcgaccagg ccctggcgct cggggtcctc
     1201 gccgatctcg ctcaggagcg tgcgcacggc gcgctccagg tcaccgccgg gtactggctc
     1261 gtccacgacc tgcatcctac cagcggtaac ccgattctcc cctatgcccg ccggggtggt
     1321 cgcggtacac ttggttcgtt cagtccgttg tccccaagca ccgccgccgg ccccatgatc
     1381 gtccacccga ttccacatcg aaccggtcgc cttccgctcc gtggtctgcc gggcatctcc
     1441 ggcgaccggc tggcgcacgc cctcggccgc cacctggacg acggccacca ctatttccgc
     1501 aaggtgcagg ccggcgggca gccgccgatc gacgcggtcc tggtcgggcc aggcggcacg
     1561 tggaccatca cccacgcgca cgtggccggc cgcttccgca agcgcaacgg acactggtac
     1621 cgctggaacc gctccaccga gtcgtggatc ccctgggatg ccaccccgct gcaccaggcc
     1681 cgcctggccg gccaccggct ggagctctac ctcgagcgcg ccaccctgcc ggccatggtg
     1741 cacgccaccc tgcttgcgga gcaccacgcc gagatcgact gggaggcgga ccaggctccg
     1801 ggggtgtacg tgtacaacga gctcgagcgg ctggcggcgc gcgtgtcgcg tgacgaggag
     1861 ctgaccccgg cccagatcga ccgcatcgtc gcgctgctgg acccgcggca gccactcccc
     1921 aggttcgcgg cctcggagtc atccggctga cgctgtccag cgtggcgggc cggatccata
     1981 ccggccctgg gctatgatcg ccggccagat gccccccgag cgctcccccg ctgccgcggc
     2041 gaccgtggcc ggcgcggtga tcgtcctgct gctggccgcc tggctgctgc ccccgctggc
     2101 cgtcgtcgtg gtctggccgc tgctgttcgt gctgcccgga tgggcgctgg tggcgtggac
     2161 gcgcccgcgg atcgggtcca ccggccgttt ggggctggcc gtcgtcctct cggtcgccat
     2221 ctccacccac ctggtgtact ggacatcgct ggccgccggc tacgagcgct ggacggtctt
     2281 cgccgtggcc gccgtgctgg ccatccccct gccgatcgcg gcctggctcg gcggcctcgg
     2341 ccgagccggc gtgttgggcg cacagctcac cgccgcctgg cacgccattc gccgcgagcg
     2401 ggtggcgttc gccttcgccg ccctggccgc ggccttcgtg ggcctggtgc tcgacgccgg
     2461 gctgtggaag cccaccgaga ccgggatcgc cgccggcgga tcgaactgga gcgacctggg
     2521 cgtgcacctg tcgatcgcgc agtcgctcaa tgcagcgaat ttcccgccgg aggtgccgta
     2581 cttcgccggg gcgccactgg tctaccactg gttcgccgac ttccatgccg ccatcgcggc
     2641 gcgcgcggcc ggcatgttcg ccgtgccggc cttcaccgtc tcgtccgcca tcctggccgg
     2701 cgcgctggcc ctgctggtcc acggcctggc tcgccggctg ctgcgcggcg gggcggcccg
     2761 gagggcggcc ttgctggcgg cggtgctcgt catcttcggc ggcgggctgg gctggatccg
     2821 ctgggtgagc gacatcgccc agggcttcgg cgaccccatc gggctcatgg tcaacaactc
     2881 ctacgacaac tactggttcg acgtccacga ccaggccccg tggccctact tccgcatccc
     2941 gtcggtgatg ggcaccggcc tgctggtgca ccgcgccacg acggccggat tgccgatgct
     3001 ggtgggcgcc gtcctgctgc ttgtcgccgg gctgcccacc gctcgccggg cgagggccgg
     3061 gtaccgcgac cgaccgatgc tgatcgggct ggccggactg ctgggcgcca tgctggcccc
     3121 cttccacttc ttcttcttcc cggccttcct gctgctggcc atgctctacg tgctgatcgg
     3181 cggcaggctg ctggacatcc gggcgccgga gaacgccttc gtgttcctgc tcccctacct
     3241 gctggcggtc ccgttcgcca gcgccgcgct gctcaacgcg cgcggatccg gctcgctgaa
     3301 gccggtcctg ggctgggagt cggctccgct ggccgacggc ccggcaggcg tcctgttctt
     3361 ctacgtgacc aacctgggcc tgccgctgct gctggcggtg gccgcgctgc tgacgccgcg
     3421 cacgccgtgg cgctggttcc tgggcgcctg ggcggcggcg ctgttcgcca tccccaacct
     3481 cgtccaggtc agcgacatcg ccttcgacat gaacaagtac ttccaggcga tgtgggtcgc
     3541 ggtcgccctg ctggccggct ggctgatgcg ccgctgggcg tggccggcca tcgcggtcgt
     3601 gctcctggtc agcatgccgt cgccgctgct ggtggccggc tggacggcat tcaaccgcga
     3661 gcaggtgatc gactactggg gcctggaggc ggcggagtgg atcgccgaga atacgcccga
     3721 ggacgccgtc ttcgccactg acggctggct gaactcgccg accgatgcgg cagggcgcaa
     3781 gcggctgctg accttcgccc cctacgtggc caacctcggc ttcgagccgg aggtccgggt
     3841 gcgccaggtg cgcgacatgt actgcgccgg cgacccggcc ctgaccgtgg acctgatgcg
     3901 gagtctcgac gcggcctacc tcctcgaggc cgggcgcccc agcccgtgcg acaacccgac
     3961 cgacttcgcc gccgcgccgg gactggagaa ggtctacgag ggcggcccca tccgcatctg
     4021 gcacaggacc gatgccgcgg cgggcacgcc cgccgctccc tgaggctgcc cgggccggga
     4081 ctggccctat catcgcgtcc gtgagcaccg cgcagcagga cccgatggcg cgcgcgtccg
     4141 gcgcggtcgt cagcctggag cacgtgacca agcgcttcgg acctcccggc acgccagcgg
     4201 ccgtcgacga cctgtcgctc accatctccg ccggcgagat cgtggtcctg gtggggccgt
     4261 ccggctgcgg caagacgacc accatgaaga tgatcaaccg cctggtggag cccacctcgg
     4321 gccgcgtcac gatcgacggc gaggacgtga tgggcctgcc gccggaggag ctgcggcgca
     4381 ggatcgggta cgtgatccag caggtcggcc tcttcccgca cctcaccgtg gccgacaacg
     4441 tggccgtcgt gccgcggctg ctgcgctgga agccagcacg catcagggag cgggtcgagg
     4501 agctgctgga gctggtcggg ctcgagccat cggtctacgc ggcgcgctac cccacggcgc
     4561 tgtccggtgg cgagcgccaa cggatcgggg tggcacgtgc gctggccgcg gacccgccgc
     4621 tgatgctcat ggacgagccg ttcggcgccg tcgaccccat ccgtcgtgac cggctgcaga
     4681 acgagtttct gcgcctgcag gagcgggtgc gcaagacggt cgtcttcgtg acccatgacg
     4741 tcgacgaggc gatcaagatg gccgaccgca tcgcgatcat gcagcgcggg ggcatcgtgg
     4801 cccagtacga cacgccggag gtggtgctca cccagccggc cagcgagttc gtcgagcact
     4861 tcgtgggcgc cgaccgcggc ctgaagcgcc tctcgctggc gcgggtgggc gatcttccgc
     4921 tcctggagcc gatgacggtg cgggtcggcg aagtggcagc cgatgcgcgc cggcgcctgg
     4981 aggaggcggc tgtggactac gccctgctgg tggatgccga ccggcggccg ctgggctgga
     5041 tcgaccctgg cgaccttgcc ggcgacggcg agatcggtcc ggaggcggcc acccccggcg
     5101 cacccaccgt gcagcctgag acgacgttgc gcgacgcgct ctcgggcatg ctggcctcgg
     5161 ccgtccagtt cggcgtggtg gtggacgagc gcgaggcggt gctgggcctg atcagcgtga
     5221 atgccatcag cgagaccctg cgcgccgcga ccgccggaga cggcggcgga tgagcgaggg
     5281 gcagccggtc atcgacgtcg actggatcgt tcgcaacctg gaggacatcg gccagcgcac
     5341 gctggaccac gtggtgctca ccgtgctggc ggttggcatc ggcctgctca tcagcttcgg
     5401 gctggcgctg ctggtccgcc gctggcgacc cctgcacgga ccggtgctgg ccgtgaccgg
     5461 aacgctgtac tcgatcccga gcctggcgct cttcagcctg ctgatcccgt tcaccggcat
     5521 ccagagcatc ctgaccgccg agatcgcctt ggtcagctac acgctcctga tcctggtgcg
     5581 caacatcgtg accggcctgg acagcgtgcc gcccgacgtg ctcgaggcgg cccaaggcat
     5641 gggctacacc ccctggcagc ggctgctgcg catcgagcac gtcgggcggc acgctgtcca
     5701 ggccggtcac gatgttgcgc accaggatca ggagcgtgta gctgaccaag gcgatctcgg
     5761 cggtcagg
//
LOCUS       DATACA010000360         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2134885, whole genome shotgun sequence.
ACCESSION   DATACA010000360 DATACA010000000
VERSION     DATACA010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134885"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VHK28_07455"
     CDS             <1..429
                     /locus_tag="VHK28_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142089.1"
                     /translation="ISLVPIGGVPEGRPNLAVFCALIIGALMGFLWFNVHPAQLFMGD
                     AGSLSLGATLAVVATITGQLPLLAVIGIVFLAVIMSVVLQVVSFKTRGRRIFRCAPLQ
                     HHFELIGWAEEKITVRFLIVAALAGLLGYSLFLASTQGLQ"
     gene            435..1844
                     /gene="murD"
                     /locus_tag="VHK28_07460"
     CDS             435..1844
                     /gene="murD"
                     /locus_tag="VHK28_07460"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX2142090.1"
                     /translation="MMTSAPPLTSTADLRGRRVVVLGLARSGVAAARMLAHAGARVTA
                     YDRRHASELAGAIAALDGLGVRTVPTATPAEVQALLASAELVVTSPSISARFPTTDEW
                     LRRALSDAQANGTPVLGEVDLFLSLTRARVLAVTGTKGKTTTVALLGSILEAAQMPHA
                     VGGNIGTPLIERVDELGADTWAVLELSELQLPSIPRGAEVAVYTNIAADHLDRHGTVE
                     AYRAVKARLAQLTVGSGTVVLNADDPGCRELGATLDGSLRWYGLDEDGLDAGSDGLHL
                     MLAGSPLLAVADVPLPGRHMLGNALGAILGAAEAGVPREAIAAGVRGFRGVPHRLETV
                     ATHRGVRYVNDSQATIPMAALAALDAFADAPLVLIAGGQGKGLEYAELADRIAQRARV
                     AVLLGETADELERLIAGRLTVRRASDMDEAVELATQAARPGEVVLLSPAATSFDMFTD
                     YAARGDAFRAAVNRLTEAS"
     gene            1841..>2922
                     /gene="ftsW"
                     /locus_tag="VHK28_07465"
     CDS             1841..>2922
                     /gene="ftsW"
                     /locus_tag="VHK28_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX2142091.1"
                     /translation="MSAGAVAVRKGSSVRIAVKRERHHPNYALLTAVLALVAVGVVMV
                     YSASSVRSYLASADPAAQGMQQAIWAALGLGAMYAASRVDFRLLRYLAIPAYVGTILL
                     LVLVLIPGIGTEAFGSRRWIVVPGVGSFQPAELAKLAIVIYLAHWLDRRGREARSFWN
                     GLVPFALLVAPGFLLIAMEPDMGTAGVYALTALSVFFMAGANLVYLTTIATAIGAAAV
                     WMISRTEYQMERVQTFLDPFRDPLGAGYNTIQGLLALGLGGIAGQGLGASKQKFLYLP
                     APSTDFIFAIIGEEWGLVGTLTVVALFVIVAYHGYRIAINAPDTFSGLLAAGITTWLV
                     TQAAINMMVVTALMPVTGIPLPFISYG"
BASE COUNT          363 a         1029 c         1051 g          479 t
ORIGIN      
        1 atctcgctgg tgccgatcgg cggggtgccc gagggcaggc ccaacctggc cgtcttctgc
       61 gcgctgatca tcggtgccct gatgggcttc ctgtggttca acgtccatcc cgcacagctg
      121 ttcatgggcg acgccggctc cctgtcgctg ggcgcaacgc tggccgtggt ggccaccatc
      181 accggccagc tgccgctgct ggcggttatc ggcatcgtct tcctggcggt gatcatgagc
      241 gtggtcctgc aggtcgtctc cttcaagacc cgcgggcggc gcatcttccg atgcgctccg
      301 ctccagcacc acttcgagct gatcgggtgg gccgaggaga agatcacggt gcgcttcttg
      361 atcgtcgcgg cgctggccgg cctgctcggc tacagcctct tcctggcctc cacccagggc
      421 ctccagtagg accgatgatg accagcgccc cgcccctcac ctccaccgcc gaccttcgtg
      481 gccggcgggt ggtcgtgctg ggcctggccc gctcgggcgt ggcggcggcc cgcatgctgg
      541 cccacgccgg cgcccgggtg acggcctacg accggcggca cgcgtccgag ctggcgggcg
      601 cgatcgccgc cctggatggc ctgggagttc gaaccgtgcc gaccgccacc ccggcggagg
      661 tccaggcact gctggcctcc gccgagctgg ttgtcaccag cccgtccatc tcagctcgtt
      721 tcccgacgac ggacgagtgg ttgcgccgcg ccctgtccga tgcgcaggcg aacgggaccc
      781 cggtgctggg cgaggtcgac cttttcctgt cactgacgcg ggcgcgggtg ctggccgtca
      841 ccggcaccaa gggcaagacc acgaccgtgg cgctgctggg ttccatcctg gaggcggccc
      901 agatgccgca cgccgtgggc ggcaacatcg ggacgccgct gatcgagcgg gtggacgagc
      961 tgggcgccga tacgtgggcc gtgctggagc tgtccgagct gcagctgccg agcatcccgc
     1021 gaggcgcgga ggtggccgtc tacaccaaca tcgcggcgga tcacctcgac cgccacggca
     1081 cggtcgaggc ctatcgagcg gtgaaggcgc ggctggcgca gctcaccgtc ggtagcggca
     1141 cggtcgtgct gaatgcggac gaccccggct gtcgcgagct gggggccacg ctggacggct
     1201 cgctgcgctg gtacgggctg gacgaggacg gcctggatgc gggcagtgac ggactgcacc
     1261 tgatgctcgc cgggtcgccg ctcctcgcgg tggccgacgt gccgctgccg ggccgccaca
     1321 tgctcggcaa cgcgctgggc gccatcctgg gcgcggccga ggcaggggtc ccccgcgagg
     1381 cgatcgccgc cggcgtgcgt gggttccgtg gcgtgccgca ccgactggag acggtggcca
     1441 cgcaccgcgg cgtgcgctac gtgaacgatt cgcaggccac cattccgatg gccgccctgg
     1501 cggcgctcga cgccttcgca gatgcgccgc tggtgctgat cgccggcggg cagggcaagg
     1561 ggctcgaata tgccgagctg gccgatcgca tcgcgcagcg ggcccgggtg gcggtgctgc
     1621 tgggcgagac ggcggacgag ctcgagaggc tgatcgccgg acggctcacg gtgcggcgcg
     1681 cgtcggacat ggacgaggcg gttgagctgg ccacgcaggc cgcccggcct ggcgaggtcg
     1741 tcctcctctc cccggcggcc accagcttcg acatgttcac cgactacgcg gcgcgcgggg
     1801 acgcgttccg cgcggccgtg aaccggctga cggaggcatc gtgagcgccg gggcggttgc
     1861 ggtgcggaag ggctcatcgg tccggatcgc ggtcaagcgc gagcggcacc accccaacta
     1921 cgcgctgctg accgcggtgc tggctttggt ggccgtgggc gtggtcatgg tctattccgc
     1981 gtccagcgtg cggtcctacc tggccagcgc cgatccggcc gcccagggca tgcagcaggc
     2041 gatctgggcg gcgcttggcc tgggcgccat gtacgccgcc agccgggtcg acttccggct
     2101 gctgcgctac ctggccatcc cggcctacgt gggcaccatc ctgctgctgg tgctggtgct
     2161 catccccggc atcgggaccg aggccttcgg ctcacggcgt tggatcgtgg tgccgggcgt
     2221 gggctccttc cagccggccg agctggcgaa gctggcgatc gtcatctacc tggcgcactg
     2281 gctggatcgg cgcggccgcg aggcgcgctc cttctggaac gggctggtgc ccttcgccct
     2341 gctggttgcg cccggcttcc tgctgatcgc catggagccg gacatgggca ccgccggggt
     2401 gtacgcgctg accgcgctgt cggtcttctt catggccggc gccaacctgg tgtacctgac
     2461 caccatcgcc accgccatcg gggcggccgc cgtgtggatg atctcgcgca ccgagtacca
     2521 gatggagcgc gtgcagacct tcctcgaccc gttccgcgac ccgctggggg ccggctacaa
     2581 caccatccag ggcctgctgg cgctggggct gggcggcatc gccggccagg gcctgggtgc
     2641 cagcaagcag aagttcctct acctgccggc gccgtccacc gacttcatct tcgccatcat
     2701 cggggaggag tggggactgg tcggtacgct gaccgtggtc gccctgttcg tgatcgtcgc
     2761 ctaccatggc taccggatcg cgatcaacgc acccgatacg ttctccgggt tgctggccgc
     2821 cggcatcacc acctggctgg tgacccaggc cgcgatcaac atgatggtcg tcaccgccct
     2881 gatgccggtc accggcatcc cgctgccctt catcagctac gg
//
LOCUS       DATACA010000361         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_448238, whole genome shotgun sequence.
ACCESSION   DATACA010000361 DATACA010000000
VERSION     DATACA010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448238"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /gene="clpX"
                     /locus_tag="VHK28_07470"
     CDS             <1..789
                     /gene="clpX"
                     /locus_tag="VHK28_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX2142092.1"
                     /translation="QRAQRGIIYIDEIDKIARKTDNPSITRDVSGEGVQQALLKILEG
                     TVANVPPQGGRKHPHQDFIQIDTTNVLFICGGAFEGLEKIVEERVGRRGIGFGSEAHR
                     TKDDRRRLMDQLMPEDLLKYGLIPEFVGRLPVVVGLTALAKEDLMKVLTEPKNAVTRQ
                     FTKFLSLDKVELVFTDEALEATARLAIARRTGARALRTIVEEALLEVMYDIPERTDVQ
                     KVVITAETIEHGQPPMLVTETEAEPRRRSAKDRERLAERAEESA"
     gene            complement(786..1679)
                     /locus_tag="VHK28_07475"
     CDS             complement(786..1679)
                     /locus_tag="VHK28_07475"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX2142093.1"
                     /translation="MTFNPELALKVAGIALLALVGVWASRVAIRAAVRHVLELRSPQD
                     EGLMTGIDFERRVQTLQTLGVRVVAGVILTVAALMAISLFGVSIAPAIAGLGVVGIAV
                     GLGAQTLIKDWLSGIFIVLENQYSVGDVVQIAGVDGVVEDFSLRRTVLRNLDGTVHSV
                     PNGNITVASNMTRGWARVNLDISVAYDTDIDRATRVIDRVGQALADDPEWRDRLIEAP
                     SVQRVNALGDSAVALKILGQVRPAQQWAVAGELRKRILAAFAAEGIEIPYPHQVMISR
                     TQPSAAGVEAPVSGDGEPAKD"
     gene            1787..>2824
                     /locus_tag="VHK28_07480"
     CDS             1787..>2824
                     /locus_tag="VHK28_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142094.1"
                     /translation="MTANPRRPAFRLPWSDESTTTTEPAADAASASAEPNPPAEPAGA
                     ATDPAAAAEATSAEPAAAESTEAAESTEFLRSLLSAMRSVADSSRESSLSELRQTVEA
                     RIAELREAAATQAEELRRLADQDVSAVEEWEKAEQERVRAEAERRRESRRAQLGRQLA
                     EHQAASQREMEEARRRLAEHERELAAFFAQLGDITDPAAFVAAAKRMPRAPELTHASD
                     APAVTATPSEPVAPEDRAEPDTSPASEEPSWPAPTPSAVVDTRLAERLAELDARLAGD
                     TATASTPAVVGHAEVNTNGETSTAIVVKGLGSFGAITSFKQALERIEGIRGVTLSLGP
                     TGEFVYRASHAA"
BASE COUNT          475 a         1038 c          954 g          357 t
ORIGIN      
        1 cagagagccc agcgcggcat catctacatc gacgagatcg acaagatcgc gcgcaagacg
       61 gacaacccgt ccatcacccg cgacgtatcc ggcgaaggcg tgcagcaggc cctgctcaag
      121 atcctggagg ggacggtggc caacgtgcca ccacagggcg gccgcaagca ccctcaccag
      181 gacttcatcc agatcgacac caccaacgtc ctcttcatct gcggcggcgc cttcgagggc
      241 ctggagaaga tcgtggagga gcgcgtcggc cgtcgcggca tcggcttcgg gtccgaggcg
      301 caccgcacca aggacgaccg gcgccggctg atggaccagc tcatgccgga ggacctgctc
      361 aagtacggcc tcatccccga gttcgtcggc cgcctgccgg tggtggtcgg cctgaccgcc
      421 ctggccaagg aggacctgat gaaggtcctg accgagccga agaacgccgt cacccggcag
      481 ttcaccaagt tcctgagcct ggacaaggtc gagctcgtct tcaccgatga ggccctggag
      541 gccaccgccc gcctggccat cgcgcgccgc accggcgccc gcgccctgcg caccatcgtc
      601 gaggaggcgc tgctcgaggt gatgtacgac atccccgagc ggaccgatgt ccagaaggtg
      661 gtcatcaccg ccgagaccat cgagcatgga cagccgccga tgctggtcac cgagacagag
      721 gcggagccgc gacgccgcag cgccaaggac cgggagcgcc tcgccgagcg cgcggaggag
      781 tcggcctagt ctttcgccgg ctcgccgtcg ccggagaccg gcgcctcgac cccggccgcg
      841 ctcggctggg tgcggctgat cataacctgg tgcgggtagg ggatctcgat gccctccgcc
      901 gcgaacgccg ccaggatgcg cttgcgcagc tccccggcca ccgcccactg ctgtgcgggc
      961 cgcacctgcc ccaggatctt gagggcgacc gcggagtcac ccagtgcatt cacgcgctgg
     1021 accgatggtg cctcgatcag gcggtcccgc cactccggat catcggccag tgcctggccg
     1081 acacggtcga tcacgcgcgt ggcgcgatcg atatcggtgt cgtaggcgac cgagatgtcc
     1141 aggttcacgc gcgcccagcc gcgcgtcatg ttggacgcca ccgtgatgtt cccgttcggc
     1201 acgctgtgga ccgtcccgtc caggttgcgc agcaccgtgc gacgcaggct gaagtcctcc
     1261 accacgccgt ccaccccggc gatctgcacg acgtcaccca cgctgtactg gttctccagc
     1321 acgatgaaga tgccggacaa ccagtccttg atcagggtct gggcacccag gcccaccgcg
     1381 atccccacaa cccccagtcc cgcgattgcg ggcgcgatac tgactccgaa aagcgagatg
     1441 gccatcaggg ccgccacggt caggatgacc ccggccacga cccgcacgcc cagcgtctgc
     1501 agggtctgca cgcgccgctc gaagtcgatg ccggtcatca gcccctcgtc ctgcggggag
     1561 cgcagctcga ggacgtggcg gacggcggca cggatcgcta cacggctggc ccagacgccg
     1621 accagggcaa gcagggcgat gcccgctacc ttgagtgcca gctcgggatt gaaggtcatg
     1681 gcgaggccat ggtagcaacg gccaggggtg gtacgaccgg tgcaggggcg ccggtacttc
     1741 gggtccctgc gtactcgccg gggcgtcccg ctaggatggg gcaacgatga cagccaaccc
     1801 ccgccgacct gcctttcgcc tcccctggag cgacgagagc accacgacca ccgagcctgc
     1861 ggccgacgcc gcgagcgcct cggctgagcc gaatccgcct gccgagccgg ccggcgcggc
     1921 cactgatccg gcagcagccg cggaagcgac ctcagccgag cctgccgccg ccgagtctac
     1981 cgaggcagcg gagtcgaccg agttcctgcg cagcctgctg tccgccatgc gctcggttgc
     2041 cgactcctcg cgcgagagca gcctgagcga gctgcgacag acggtcgagg cccgcatcgc
     2101 cgagctgcga gaggcggccg ctacccaggc cgaggagctg cgccggctgg ccgaccagga
     2161 cgtgtccgcg gtcgaggagt gggagaaggc cgagcaggag cgcgtacgag ccgaagcgga
     2221 gcgccgacgg gagtcgcgcc gggcgcagct gggacggcaa ctcgccgagc accaagctgc
     2281 cagccagcgc gagatggagg aggcgcgtcg ccgcctggca gagcacgagc gggagctcgc
     2341 cgcgttcttc gcgcagctgg gagacatcac cgatccggcc gccttcgtgg cggccgccaa
     2401 gcgcatgccg cgcgccccgg agctcacgca tgcttccgac gcgcctgcgg tgactgcgac
     2461 cccaagcgaa ccggtcgccc ccgaagaccg tgccgagccg gacacctcac cggcgtccga
     2521 ggagccttcc tggccggccc ccacgcccag cgccgtcgtc gacacgcgcc tggccgagcg
     2581 cctggccgag ctggacgcgc gcctggccgg cgacacggcc acggccagca cgccggcggt
     2641 ggtcgggcac gcagaggtga acaccaacgg cgagacctcg accgccatcg tggtcaaggg
     2701 gctgggcagc ttcggcgcca tcacctcctt caagcaggcg ctcgagcgca tcgagggcat
     2761 ccgcggcgtc accctgtcgc tgggccccac cggagagttc gtataccgcg ccagccacgc
     2821 cgcc
//
LOCUS       DATACA010000362         4004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_448303, whole genome shotgun sequence.
ACCESSION   DATACA010000362 DATACA010000000
VERSION     DATACA010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448303"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VHK28_07485"
     CDS             <1..206
                     /locus_tag="VHK28_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142095.1"
                     /translation="VLEVMRELAGTGMTMLVVTHEMGFARAVANRMVFFDHGQIVEQG
                     PPTQIFEAPEQDRTKQFLSQILH"
     gene            complement(552..794)
                     /locus_tag="VHK28_07490"
     CDS             complement(552..794)
                     /locus_tag="VHK28_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142096.1"
                     /translation="MHDVTGNRLLLWLAFTAGGVLIGFGLAFVAASAASLVLPHCGIE
                     CDDSVPELVAAVATYGTWALAAVTTSVLAWREISNR"
     gene            1038..1709
                     /gene="folE"
                     /locus_tag="VHK28_07495"
     CDS             1038..1709
                     /gene="folE"
                     /locus_tag="VHK28_07495"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2142097.1"
                     /translation="MTATHSTNGSSPPVTPAWLSQLELRAIGQEERQRFEGYMTEIFT
                     ALGMHLSTPGTAETPRRFVQAMIDATDGYEGDPKLVTIFPTECHGDAACELAQIVEGP
                     IPFYSLCEHHALPFFGQAYVGYVAHDQIIGISKLTRLVRVLTRRFGVQERMSHEIAAS
                     LSELLNAHGVAVYMEADHLCTQMRGVREDKATTRTTAYRGVYERDPALRGEFFQVAGI
                     GRTGR"
     gene            1709..2584
                     /locus_tag="VHK28_07500"
     CDS             1709..2584
                     /locus_tag="VHK28_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX2142098.1"
                     /translation="MLAVPLAAPTRLTPLVSDAPDSARVRGGSLPSALEDRYGGPLAI
                     ALRTGRPTVVANFVSTLDGVVSYNTPEAAGGGEISGFFEPDRFVMGLLRAVADVVLIG
                     AGTLRAGVGETWTPESVHPASAAAYAALRAGLGLRPRPVTAVVTASGSVDLDHPGLAD
                     PGTDVLLITTDAGAAALAHQQPPAHVQVRAIGGAVRTEDILRLLADAGARVVLCEGGP
                     HLLGQLLRDRLVDELFLTLAPQIAGRSDVLARPGLAEGVAFDVASAPWAMLTDLRRAG
                     DHLFARYRLKEEPGS"
     gene            2581..3801
                     /locus_tag="VHK28_07505"
     CDS             2581..3801
                     /locus_tag="VHK28_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2142099.1"
                     /translation="MNKLNSVVIVGGGLAGASAAFALRDAGFAGRVVLVSEEQEPPYE
                     RPPLSKAYLRGEQPLEKAFVRPLADYETQGIELLSGRRAVTLDPPARSLNLDDGTNLP
                     YDALLLATGAEPRRLGSTQAQVPGVHYLRTVHDAAALRGAAVQADAIVVIGGGWIGSE
                     VAASLRQLGRNVTLISNLPRPLERVLGPEVADAYRTLHLENGVRLEQGRVAVIDGTER
                     VTGVLLSDGRRLAADLVVVGIGAQPRLKLAMRAGLETMDGAIAVDEHLRTSVPSIYAA
                     GDVAAAWHPRFGRHLRVEHWDNAIHQGRLAAANMLGANQAYTRTPYFYSDQFDLGMEY
                     RGYAPQWGSVVIRGDLAARQFHAFWLADGRVVAAMNANLWDDGAELQRLVESEERVDL
                     ARLADPGVPLAEAA"
     gene            complement(3923..3998)
                     /locus_tag="VHK28_07510"
     tRNA            complement(3923..3998)
                     /locus_tag="VHK28_07510"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3963..3965),aa:Ala,seq:cgc)
BASE COUNT          590 a         1430 c         1380 g          604 t
ORIGIN      
        1 aggtcctcga ggtcatgcgc gagctggccg gcaccggcat gaccatgctg gtcgtcaccc
       61 acgagatggg cttcgcgcgg gccgtggcca accgcatggt cttcttcgac cacggccaga
      121 tcgtggagca ggggccgccc acgcagatct tcgaggcgcc agagcaggac cgcaccaagc
      181 agttcctctc gcagatcctc cactaggggc tgcggcgggt cgggcggctc ctcacgcggc
      241 cgctgccgca ggcgcttctt ttcgtcagat gtcgccctgg tgagccttac caggctactc
      301 ggcagtgctt cctggtctag tgctcacctg cggccccttg cgcgactatc ggctgcgttg
      361 cgatgcccgg tctggcgatg tcgagcgtgc gaggccctcg cgcacttcgg ctactgctta
      421 ccaggtgcgc gtttgaccag gatttgatgc caacccgcgc gataaggagc gctggctgag
      481 cgtatgacga acttcgtcgt cgccagctgc ctcaagtccc gggacatgtt gcgccgggtg
      541 atgagcgggg actatcggtt cgagatctcg cgccaggcga gcacgctggt agtcaccgcc
      601 gccagggccc aggtgccgta ggtcgcaacc gccgccacca actccggcac gctgtcgtca
      661 cactcgatgc cgcagtgggg cagcaccagg ctggcggccg aggccgccac gaaggccagc
      721 ccgaagccga tcagtacgcc gcccgcggtg aaggcgagcc acaggaggag tcggttgccg
      781 gtcacgtcat gcacgacgcg attgtggccc taagtgttgc gcccgcgcgc caattctccg
      841 cgcgctccca gccggcgacg cccgctctat ttcacgctca gactaccgat agagttgaca
      901 gcctcgccct gcttcttgta tcactaacac acgctaacga tagaaactgg tcgccaccac
      961 ggtggccggg catcgggagc tgtggtgcgg cgagctgtgc gtcgcaccgc cgctctcact
     1021 tcagcacagg agcagagatg acggccaccc attccaccaa tggcagctcg cccccggtca
     1081 caccggcctg gttgtcccag ctcgagctgc gggccatcgg gcaggaggag cggcagcgtt
     1141 tcgagggata catgaccgag atcttcacgg ccctcggcat gcatttgagc acccccggga
     1201 cggcggagac gccgcgccgc ttcgtgcagg ccatgatcga cgccaccgac gggtacgagg
     1261 gcgaccccaa gctggtcacc atcttcccca ccgagtgcca cggcgacgcc gcctgtgagc
     1321 tggcgcagat cgtcgagggc ccgatcccgt tctactcgct ctgcgagcac cacgcccttc
     1381 cgttcttcgg gcaggcgtac gtgggatacg tggcgcacga ccagatcatc ggcatcagca
     1441 agctgacccg gctggtgcgg gtgctcaccc gccgcttcgg cgtgcaggag cggatgagcc
     1501 acgagatcgc cgcgtccctg tcggagctcc tcaacgcgca tggggtcgcc gtgtacatgg
     1561 aggccgatca cctgtgcacc cagatgcgcg gcgtgcgcga ggacaaggcc accacccgga
     1621 ccaccgccta ccggggcgtc tacgagcgcg accccgccct gcgcggcgag ttcttccagg
     1681 tcgcaggcat cggacggacc gggcgctgat gctcgccgtg ccgctcgccg cgcccacgcg
     1741 gctgaccccg ctcgtctccg acgctcccga ttcggcccgg gtccgcggcg gcagcctgcc
     1801 gtcggcgctc gaggaccgat acggagggcc gctcgccatc gccctccgga cgggtcggcc
     1861 caccgtggtg gccaacttcg tctccaccct ggacggtgtc gtctcctaca acaccccgga
     1921 ggccgccggc ggtggcgaga tcagcggctt cttcgagccc gaccgcttcg tgatgggcct
     1981 cctgcgtgcg gtggccgacg tcgtcctcat cggcgccggg acgctgcgcg cgggggtcgg
     2041 cgagacatgg acgcccgagt ccgtgcaccc ggcgagcgcg gccgcatacg cggcgctgcg
     2101 cgccgggctg ggcctgcgtc cgcggccggt caccgcggtg gtcaccgcct ccggatcggt
     2161 cgacctcgac catcccggcc tggccgaccc agggaccgat gtgctgctga tcaccaccga
     2221 tgcgggcgcg gccgcactgg cccaccagca accgcctgca cacgtccagg tgcgtgccat
     2281 tggcggggcc gtccggaccg aagacatcct gcggcttctc gccgacgccg gcgccagagt
     2341 ggtcctgtgc gaaggcgggc cgcacctgct cggacagctg ctccgcgacc ggctcgtgga
     2401 cgagctgttc ctgacgctcg cgccccagat cgccggacgc tccgacgtgc tggcgcggcc
     2461 ggggctcgcc gaaggcgtcg ccttcgatgt cgccagcgcg ccgtgggcaa tgctcaccga
     2521 cctgcgacgg gccggtgacc acctcttcgc tcggtatcgc ctgaaggagg aacctgggtc
     2581 atgaacaagc tcaactcggt cgtgatcgtg ggcggcggcc tggcgggcgc cagcgcggcc
     2641 ttcgccctgc gcgacgccgg cttcgccggg cgcgtcgtgc tcgtcagcga ggagcaggag
     2701 cccccgtacg agcggccgcc actgtccaag gcctacctgc gtggcgagca gccgctcgag
     2761 aaggccttcg tgcgccccct ggccgactac gaaacgcagg ggatcgagct gctcagcggc
     2821 cggcgggcgg tgacgctcga tccgccggcc cgcagcctga acctggacga cggaaccaac
     2881 ctgccgtacg acgccctgct gctggccacg ggcgccgaac cgcgtcggct gggatccacc
     2941 caggcacagg ttcccggcgt ccactacctg cgcaccgtgc acgacgccgc tgccctccgc
     3001 ggcgcggccg tgcaggcgga tgcgatcgtg gtgatcggcg gcggctggat cgggagcgag
     3061 gtggcagcgt cgcttcgtca gctgggacgc aacgtgacgc tcatctccaa cctgccgcgg
     3121 ccgctggagc gggtgctggg cccggaagtg gctgatgcct accgcacgct ccacctggag
     3181 aacggcgtgc ggctggaaca gggccgcgta gccgtcatcg atgggaccga gcgcgtcacc
     3241 ggcgtgctcc tgagcgacgg gcgacgcctg gcggccgacc tggtggtggt cggcatcggt
     3301 gcccaacccc gcttgaagct ggccatgcga gccgggctgg agacgatgga cggcgccatc
     3361 gcggtcgacg agcaccttcg gacatcggtc cccagcatct acgccgccgg cgacgtggcc
     3421 gccgcctggc acccgcgctt cggtcgccac ctgcgggtgg agcactggga caacgccatc
     3481 caccaggggc ggctggcggc ggccaacatg ctcggtgcca accaggccta cacgcgcacc
     3541 ccgtacttct actcggacca gttcgacctg ggcatggagt accgcggcta cgccccgcag
     3601 tggggcagtg tggtcatccg cggcgacctg gccgcgcgcc agttccacgc cttctggctg
     3661 gccgacgggc gggtggtggc ggccatgaac gccaatctgt gggacgacgg cgccgagctc
     3721 cagcgcctgg tcgagtccga ggagcgcgtg gacctggcac ggctggccga tccgggggtt
     3781 ccgctcgcgg aagcggcctg agcaggatgc gggcctggtc tgcgcctgtc aggggtcggt
     3841 tgtgcacccg tgcaaccgtg cggctgtgct gcacagcagg gcactgacag cgcaagagaa
     3901 gggccgggcc cggcacgggt agtggtggag atgaggggac tcgaacccct gacccctgcg
     3961 atgcgaacgc agtgctctcc cagctgagct acatccccac gggc
//
LOCUS       DATACA010000363         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_850407, whole genome shotgun sequence.
ACCESSION   DATACA010000363 DATACA010000000
VERSION     DATACA010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850407"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VHK28_07515"
     CDS             <1..885
                     /locus_tag="VHK28_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142100.1"
                     /translation="LVVAPEDAAAQLRAGEQTEIGVAWNEIDPVYDSLAHLAVATMVS
                     ELNAEIIRQGAAEGISIAEGELGPRVENISPEVLAKPTTAVTQNVSPTPPSVINFFSP
                     AVLALVLQHLAVTLTALSMVRERISGQMDLFRVAPVSSTEVLLGKYLAYALLSLFVTV
                     VVGALMIFVFGVPVLAGYLPAIGIVLLLTFASLGVGLLISLIADSERNAVQLSMLVLL
                     ASVFFSGFVLPVEDFIGPVQYVAYALPVTHGIQTLQEAMLRGAISSWWMVVVLFGIGA
                     VLYLLTLWRLRRVLRPAR"
     gene            complement(901..1509)
                     /locus_tag="VHK28_07520"
     CDS             complement(901..1509)
                     /locus_tag="VHK28_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142101.1"
                     /translation="MLGWLLLAYAAIGALLVAVALAIGGPLVARVERLTSSATETISA
                     ATDAAQSAADSFQGFDSSLEQARSSAGEAASLSRDTAITLDGLAQAMTISIFGAQPLE
                     PLAGRFETSASQLRQMGDSLDEIGEALGTNREDVSEVGLELQDLAVELRRLQGRISAE
                     QRSTDLPLSWLYYGFLLWQLLPIAAAAVGGGILLRRPRPVTI"
     gene            1563..2921
                     /locus_tag="VHK28_07525"
     CDS             1563..2921
                     /locus_tag="VHK28_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX2142102.1"
                     /translation="MAARLPGRLLARIVPRGGMVLSVLLFGSYGMGLVRDRIFARTFG
                     AGSELDAYNAAFVLPELLLDVLVEAGLAAPFIPIFVRLRSVDDGGEADRFARAILTAA
                     VTIMGVAAVVMFVFAEATTGFIAPGFTGAERALYVDLFRVMLATPILFAASLTLGQVL
                     LAEQRFVWYGIAPLLYNAGIILGTVAFSGSLGIYGPAIGAVVGAAIHLGSRFIGLRKS
                     RFRIGLGWTSRTDSIREFVRLMLPKMVSHPVEPMTFLFFTNVASGLAAGSVTVVSYAR
                     NFQSVPVTLIGVAFALAVFPALSTAHATGDRRGFLRLLVTNTASITLITTIGAIGVVV
                     VGELAIRVLLGGGAFDEQDVVRTGTVLAAFALAIPFESLTHLLSRAIYATRHTLLQVL
                     ASLAGLGITVVATLILLPEAGVVAIPLGFAIGQAAKVGLLALALAIRLRPGTSGWGGS
                     SP"
     gene            complement(2918..>3788)
                     /locus_tag="VHK28_07530"
     CDS             complement(2918..>3788)
                     /locus_tag="VHK28_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142103.1"
                     /translation="VWTNTLSARDRLDVWMSRIQAVIDPPPDRSIPPTVVVTPRPSDT
                     AAPTASPLPTASDAPTAEPSPTPPPREPVDVALVSDHAGVFVSQQTDKWCAVAGTQIV
                     LAVLGLGDNSPAFQAEIASRIGEWESWDDSHNGGWGPDAVRQALAAYGADGYEERAYE
                     SYTDALRDSAIAISTMHKPVVMFPWWGAHTWVMTGYRADADPALFPDAHVSGAYILDP
                     WYPRNSNIWGQSDGPGNFEDLGELERNWPVHQGPPGYENIGPGWSRPEGAYPDRDGRF
                     IVLLPTTPLEGDG"
BASE COUNT          491 a         1327 c         1333 g          637 t
ORIGIN      
        1 ctggtcgtgg cacccgagga tgccgcggcg cagctgcgcg ccggcgagca gaccgagatc
       61 ggcgtggcct ggaacgagat cgacccggtc tacgacagcc tggcccacct ggcggtggcc
      121 accatggtca gcgagctgaa cgccgaaatc attcgccagg gcgcggccga ggggatcagc
      181 atcgccgagg gcgagctcgg tccccgggtg gagaacatct ccccggaggt gctggccaag
      241 ccgaccaccg cggtcaccca aaacgtgtcg ccgacaccgc cctcggtcat caacttcttc
      301 tcgccggcgg tgctggccct ggtcctccag cacctggcgg tcacccttac cgcgctgtcc
      361 atggtgcgcg agcgcatcag cggccagatg gacctcttcc gggtcgcgcc ggtcagctcc
      421 accgaggtgc tgctgggaaa gtatctggcc tacgcgctgc tcagcctgtt cgtcaccgtg
      481 gtggtcggcg cgttgatgat cttcgtcttc ggagtgccgg tgctggccgg ctacctgccg
      541 gccatcggga tcgtgctgct actgaccttc gcttcgctgg gggtgggcct gctgatctca
      601 ctgatcgccg actcggaacg caacgcggtg cagctttcca tgctggtgct gctggcgtcg
      661 gtcttcttca gcggcttcgt gctgccggtc gaggacttca tcggccccgt gcagtacgtc
      721 gcctacgcgc tgccggtgac ccacggcatc cagaccctcc aggaggccat gctgcgcggg
      781 gccatcagca gctggtggat ggtggtcgtc ctgttcggga tcggggccgt cctctacctg
      841 ctcacgctgt ggcgcctgcg ccgggtgctt cgcccggccc gatgacggag cggacggcgg
      901 tcagatggtg accggccgcg ggcgccggag caggatcccg cccccgacgg ccgccgccgc
      961 gatgggcagc agctgccaga gcaggaaacc gtagtagagc caggagagcg gcaggtccgt
     1021 gctgcgctgc tcggccgaga tgcgtccctg cagtcgccgc agctcgaccg ccaggtcctg
     1081 cagctcgagc cccacctcgg agacgtcctc ccggttggtc cccagcgcct cgccgatctc
     1141 gtccaggctg tcgcccatct gccgcagctg gctggcgctg gtctcaaaac ggccggccag
     1201 gggctcgagc ggctgcgcgc cgaagatgga gatggtcatt gcctgcgcga gcccgtcgag
     1261 ggtgatggcc gtgtcccggc tgagcgacgc agcctcgccg gccgacgacc gcgcctgctc
     1321 caggctggaa tcgaagccct ggaagctgtc cgcggcgctc tgcgccgcgt cggtggcggc
     1381 cgatatcgtc tcggtggccg aggaggtgag gcgttccacg cgcgccacca gcgggccgcc
     1441 gatggccagc gccacggcca ccaacagggc accgatggcg gcgtaggcca gcaggagcca
     1501 cccgagcatg cgcatgctcg cccggcacga agcaggagac gtgccaccgc taggatgcgg
     1561 tgatggctgc gcgccttccc ggacggctgc ttgcccgaat cgtgcccagg ggcggcatgg
     1621 tgctgtccgt gctcctgttc gggagctacg gcatgggact ggtccgcgac cggatcttcg
     1681 cgcgcacttt cggcgccggc tccgagctgg atgcctacaa cgcggccttc gtgctgccgg
     1741 agctgctgct cgacgtcctg gtggaagccg gcctggcggc cccgttcatc ccgatcttcg
     1801 tccgcctccg ctcggtggac gatggcggcg aggcggaccg cttcgcccgc gccatcctga
     1861 ccgccgcggt caccatcatg ggcgtggccg cggtggtcat gttcgtcttt gcggaggcca
     1921 ccaccgggtt catcgcccct ggcttcacag gggccgagcg ggcgctgtac gtggacctct
     1981 tccgcgtcat gctggccaca cccatcctgt tcgccgcgtc cctgacgctg gggcaggtgc
     2041 tgctcgccga gcagcgcttc gtgtggtacg ggatcgcgcc gctcctgtac aacgccggga
     2101 tcatcctggg caccgtcgcc ttcagcggct cgctggggat ctacgggccg gccatcggag
     2161 cggtggtcgg cgccgccatc cacctgggca gccgtttcat cgggctgcgg aagagccggt
     2221 tccggatcgg gctgggctgg acctcgcgca ccgactcgat ccgcgagttc gtgcggctga
     2281 tgctgcccaa gatggtgagc cacccggtgg agcccatgac cttcctgttc ttcaccaacg
     2341 tggcgtccgg actggcggcc ggcagcgtga cggtggtgag ctacgcccgc aacttccaga
     2401 gcgtgccggt gacgctgatc ggggtggcct tcgcgctcgc cgtcttcccg gcgctgtcga
     2461 ccgcgcatgc caccggcgac cgacgtggct tcctgcggct gctcgtcacc aacacggcca
     2521 gcatcacact gatcaccacc atcggcgcca tcggcgtggt ggtggtgggt gagctggcca
     2581 tccgggtgct gctgggcggc ggtgccttcg acgagcagga cgtggtgcgc accggcactg
     2641 tgctcgccgc tttcgccctg gccatcccct tcgagagctt gacccacctg ctcagccggg
     2701 ccatctacgc cactcgccac acgctgctgc aggtgctcgc ctcgctggcc ggcctgggga
     2761 tcacggtggt ggccaccctc atcctgctgc ctgaggcggg cgtggtggcc attccgctcg
     2821 gcttcgcgat cggccaggcg gccaaggtgg ggctgctggc gctggcgctg gccatccgcc
     2881 tgcgcccggg caccagcggc tggggaggca gcagccccta accgtcgccc tcgagcggcg
     2941 tggtcggcag caggacgatg aagcgaccgt cgcgatccgg gtaggcgccc tccgggcgcg
     3001 accagcctgg accgatgttc tcgtaacccg gcggtccctg gtgcaccggc cagttgcgct
     3061 ccagctcgcc caggtcctcg aagttgccag gcccgtccga ttgtccccag atgttcgagt
     3121 tgcgcgggta ccaggggtcc aggatgtagg cgccgctgac gtgcgcgtcg gggaagaggg
     3181 cgggatctgc atcggcccgg tagcctgtca tcacccaggt gtgcgcaccc caccagggga
     3241 acatgaccac cggcttgtgc atggtgctga tggcgatggc cgagtcgcgc agcgcatcgg
     3301 tgtaggactc gtaggcgcgc tcctcgtagc catccgcccc gtatgccgcc agcgcctgcc
     3361 gcacggcatc cgggccccag ccgccgttgt ggctgtcgtc ccagctttcc cattcgccga
     3421 tgcgcgacgc gatctccgcc tggaaggcgg gcgagttgtc gccgagcccc aggactgcga
     3481 gcacgatctg ggtcccggcc accgcgcacc acttgtcggt ctgctggctg acgaacaccc
     3541 cggcgtggtc gctgaccagc gccacgtcca ccggctcacg gggaggggga gtggggctgg
     3601 gttccgcggt cggcgcatcg ctggcggtcg ggagcgggct ggcggttggc gcagcggtgt
     3661 cggagggccg cggcgtgacc accacggtgg gaggaatgct gcggtccggc ggcggatcga
     3721 tcaccgcctg gatgcgcgac atccacacgt cgagccggtc gcgcgcgctc agggtgttcg
     3781 tccacacc
//
LOCUS       DATACA010000364         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_367921, whole genome shotgun sequence.
ACCESSION   DATACA010000364 DATACA010000000
VERSION     DATACA010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367921"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1322)
                     /locus_tag="VHK28_07535"
     CDS             complement(114..1322)
                     /locus_tag="VHK28_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2142104.1"
                     /translation="MTEGSLPLIELLQKAGEGDFLRAVAEAVLQLLMEADVEGLIGAG
                     RHELSPERLNWRNGYRERALDTRLGTLQLRVPKLRQGSYFPPFLEPRKTSERALVAAI
                     QEAWIGGVSTRRVDELVQAMGLAGIAKSTVSKLCKEIDERVSGFLDRPLEGEWPYLWL
                     DATYLKVREGGRIVSVAAIIAVAVDAEGRREIVGLGLGPSEAETFWSGFLKGLVRRGL
                     KGVKLVISDAHDGLKHAIAKVLGATWQRCRVHWIRNALAHVPKGQQTMVAAALRQAFL
                     QPDQDAARQAWRQLADQLRPRWPKLSGLMDASEHDVLAYLAFPAQHRAKLHSTNPLER
                     LNKEVKRRADVVGIFPNEASILRLLGAVLLEANDEWQTGHRYMQLEGMAELFSPDPAA
                     ESLRLPPQAA"
BASE COUNT          236 a          542 c          508 g          238 t
ORIGIN      
        1 aagagccgcc ggccaagccg cgcgaaccgc tggcggactg caagtccgcg gtcgagcgcg
       61 ggtcgcgtcc gtcaaggagg tgtaaattcg gtagggtctg gggtggtcat cggtcaggcc
      121 gcttggggtg gaagtctcag gctctcggcc gcggggtcgg gcgagaacag ctcggccata
      181 ccttcgagct gcatgtagcg gtggccggtc tgccactcat cgttcgcctc cagcaggacc
      241 gcgccgagca gacgcaggat cgaggcctcg ttggggaaga tgccgaccac atcggcccgc
      301 cgtttgacct ccttgttcaa ccgttcaaga gggttcgtgg agtgcagctt ggcccggtgc
      361 tgcgccggga aagccaggta ggccagcacg tcatgctcgc tcgcgtccat caggcccgaa
      421 agcttgggcc agcgaggccg aagctggtcg gcgagctgcc gccaagcctg ccgggcggcg
      481 tcctgatccg gttgcaggaa ggcttggcgg agcgcggcgg cgaccatcgt ctgctggccc
      541 ttgggcacgt gcgccaaggc gttcctgatc cagtgcacgc ggcacctttg ccaggtggcg
      601 cccaggacct tggcgatcgc gtgcttgagc ccatcgtgcg cgtcggagat caccagcttg
      661 acgcccttga gcccgcgccg caccaagccc ttgaggaagc ccgaccagaa ggtctccgcc
      721 tcggaggggc cgaggcccag gccgacgatc tcgcgccggc cttcggcatc caccgccacc
      781 gcgattatcg ccgccaccga gacgatgcgg ccgccctcac ggacctttag gtaggtcgcg
      841 tcgagccaga gatacggcca ctccccttcc agggggcgat ccaaaaagcc gctcacgcgc
      901 tcgtcgatct ccttgcacag cttggagacc gtgctcttgg cgatgccggc gaggcccatc
      961 gcctgcacga gctcgtccac ccgccgggtc gagacgccgc cgatccaggc ttcctggatg
     1021 gccgcgacca aggcccgctc gcttgtcttc ctaggctcga ggaagggcgg gaaataggag
     1081 ccctgccgga gcttgggcac gcgcagctgc aaggtgccga gccgggtgtc gagcgcccgc
     1141 tcgcggtagc cgttgcgcca gttgagccgc tccggtgaca gctcgtgccg gccggcgccg
     1201 atcaggccct ccacgtccgc ctccatcaag agctgcagca cggcctcggc taccgcccgc
     1261 aggaagtcgc cctcgccggc cttctgcaag agctcgatca gtggcaggct gccctcggtc
     1321 atcggtcctc tccgtccagg tgaagcgttg cacctccacc ctagccggcc gggccgatgg
     1381 ccacctcgcc ctacaccccc acgggcaccg aaattccacc accaccgcgg acgctaccct
     1441 ggatcggcgg cgtgtccacc cggcgcgtgg acgacctcgt gcaggccatg ggcctcgccg
     1501 gcatcgccga gagcacggtc tcga
//
LOCUS       DATACA010000365         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_609114, whole genome shotgun sequence.
ACCESSION   DATACA010000365 DATACA010000000
VERSION     DATACA010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609114"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VHK28_07540"
     CDS             complement(<1..360)
                     /locus_tag="VHK28_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142105.1"
                     /translation="MRRALLLAWRLHRWELVALLVGAAGVSMVGVLTAVRLAELLAEC
                     RAAAVVVIEPCGALAEMGIISDPATTGRGFATITVLAVLPFVAGVVLGAPLLAREFEH
                     GTTLLAWPLARSRVRWLV"
     gene            complement(360..1367)
                     /locus_tag="VHK28_07545"
     CDS             complement(360..1367)
                     /locus_tag="VHK28_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142106.1"
                     /translation="MNSAVRWATLSFRLQRWEVAASVVGVLLLVSAMAWFTWHLRDAG
                     PAQLACQQSASTSDCMPALMRFDQLTDTATRLLYLSWAAPFGMGLLLGVPLVAREVEN
                     RTSAIAWTLSRSRLAWLGSRLAFVGLVLVVLLAAIAVASEALATALLPTLDLDADFTW
                     HGRRGWLVVARGLAALGIGLLVGALIGRVLPALLAAAFASVLIFTAVSIGMDRWNESE
                     AVLSAFAAGPEMGDSLAGGLTIGGRIELASGELVSWEYLNRQGYEQQMIDMDGAIYTR
                     FDEQTGEPDESSFIGWDRMLVVPGPMYPVVVLRESGVATGVALLFGLLAARVVVRRRV
                     G"
     gene            complement(1364..>2353)
                     /locus_tag="VHK28_07550"
     CDS             complement(1364..>2353)
                     /locus_tag="VHK28_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142107.1"
                     /translation="SVMATLRWLRLDFRLQRFEIVGMLSLVAVLALVTALSAVQLNEL
                     QQVHGACYVDGEVGAECLAAFEERALWAEAAHWILRAGWAVPFLFGIVLGVPIVAREI
                     EHRTGALAWSLGRSRRRWLLGRVLPVLAIVLLAAGITGVLADFAASAVNTSTVRDLSH
                     SWLWFDQRGLLVPARAAALLGVGVLVGAVVGRTLPALLVAGAVSWAVFAGTTTAMDTW
                     RITDATDIVWDSADDLESAYVIDVRVRAPDGRLLSFAEADAEQIIPAMFTTPTQEGYE
                     APPPGYTYGIEVARAVPGAEYPKWVLRESAVWAIVAGVASCLALAAARRWRPA"
BASE COUNT          431 a          883 c          767 g          272 t
ORIGIN      
        1 cacgagccat cggactcgcg agcgtgccag cggccaggcg aggagagtcg ttccgtgctc
       61 gaattcacgc gccagcagcg gcgcaccaag caccacgccg gccacgaacg ggaggaccgc
      121 cagcacggtg atggtggcga accccctccc cgtggtcgcc gggtcgctga tgatgcccat
      181 ctcggccaac gcgccgcacg gctcgatcac gaccaccgcg gccgcccggc attccgccag
      241 cagctcggcc agccgcaccg cggtcaggac cccgaccatg ctcacgccag ccgcgccgac
      301 cagcagcgcg acgagctccc agcggtggag ccgccaggca agcaggagtg cgcgccgcat
      361 cacccgactc tccggcggac gaccacgcgc gctgcgagga gcccgaacag cagcgcaacg
      421 ccggtcgcca cgcccgattc gcgcagcacg accaccgggt acatcgggcc gggcaccacc
      481 agcatccgat cccaaccgat gaagctggac tcgtccggct caccggtctg ctcgtcgaac
      541 ctggtgtaga tcgccccgtc catatcgatc atctgctgct cgtagccctg gcggttgagg
      601 tactcccagc tcaccagctc gccggaggcc agctcgatcc gcccaccgat cgtcagtcca
      661 ccggccagcg aatcccccat ctcggggccg gcggcaaacg cggacaggac ggcttcggac
      721 tcgttccatc ggtccatgcc aatgctgacc gcggtgaaga tcaggacgga tgcgaaggcg
      781 gctgcgagca gggccggcag cacacgaccg atgagtgcgc ccacgagaag accgatgccc
      841 agcgcggcca ggccgcgcgc caccacgagc caaccgcggc ggccgtgcca tgtgaagtcg
      901 gcatccaggt cgagcgtggg aagcagggcg gtcgccagcg cctcgctggc cacggcgatg
      961 gccgccagca gcaccacaag gaccagcccc acgaaggcga ggcgcgaccc gagccaggcc
     1021 agccgcgagc gactcagcgt ccaggcgatg gcgctcgtcc ggttctccac ctcgcgggcc
     1081 acaagcggca caccgaggag caggcccatg ccgaacggag cggcccagga aaggtagagg
     1141 aggcgcgttg ccgtatccgt gagctgatcg aatctcatga gtgccggcat gcagtcgctc
     1201 gtcgaggcgg actgctgaca ggccagctgc gcagggccag cgtcccgcag gtgccaagtg
     1261 aaccaggcca tggcactcac caggaggagc acgccgacga cggacgcggc gacttcccag
     1321 cgctgcagcc ggaacgagag ggtcgcccag cgcacggccg aattcatgcc ggcctccatc
     1381 gccgagccgc ggccagcgcc aggcaacttg cgaccccagc cacgatggcc cagaccgccg
     1441 actcgcgcag gacccacttc gggtactccg ccccagggac ggcacgcgcg acctctatgc
     1501 cgtaggtgta gccgggaggc ggtgcctcgt agccctcctg cgtcggcgtc gtgaacatgg
     1561 ccgggatgat ctgctcggcg tccgcctcgg cgaaagacag cagccttccg tccggcgccc
     1621 gcactcgaac gtcgatgacg tatgccgatt ccaggtcgtc ggccgagtcc cacacgatgt
     1681 ccgttgcgtc ggtgattcgc caggtgtcca tggccgtggt ggtccccgcg aagacggccc
     1741 agctcacggc tccggccacg agcagtgcgg gaagcgtgcg cccgaccacg gcacccacca
     1801 gcacgccaac ccccagcagc gcggccgcac gcgccggcac cagcagccct cgctgatcga
     1861 accaaagcca gctgtgtgac aggtcccgca cggtgctggt gttcacggcc gaggccgcga
     1921 aatcggcgag cactcctgtg atgccggcgg ccagcagcac gatggccagg accggcagca
     1981 cgcggccgag cagccagcga cgccggctgc gtccgagcga ccaggccagg gcaccggtcc
     2041 ggtgctcgat ctcacgcgcc acgatgggca cgcccagcac gatcccgaac aggaacggca
     2101 ctgcccagcc ggcgcgcagg atccaatggg cggcctccgc ccagagcgct cgctcctcga
     2161 aggcggcgag gcactcggcg ccgacctccc cgtccacgta gcacgcgccg tggacctgct
     2221 gcaactcatt cagttgcact gcggacagcg ccgtcacgag cgccaggacc gctaccagac
     2281 tgagcatccc tacgatctcg aagcgctgga gacggaagtc gagccgcagc catcgcagcg
     2341 tggccatcac gct
//
LOCUS       DATACA010000366         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1814602, whole genome shotgun sequence.
ACCESSION   DATACA010000366 DATACA010000000
VERSION     DATACA010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814602"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VHK28_07555"
     CDS             <1..357
                     /locus_tag="VHK28_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX2142108.1"
                     /translation="MLADSDRTAELQDAHDRMLALGAHAVARKVAGQLRRVGGAVRRG
                     PRTATRGNPARLTERQMEVARLVAQGRSNRDIAAHLVLTEKTVGHHVSAVLSKLDVQR
                     RAEVAGALSEFATSQR"
     gene            443..694
                     /locus_tag="VHK28_07560"
     CDS             443..694
                     /locus_tag="VHK28_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX2142109.1"
                     /translation="MTLFMDVHSIDGGVAARDVAQAHIADLKTQDRYGVRYLRYWVNE
                     GDGKVFCLVDAPDADAAKRVHREAHGLVADAIFPVVEGD"
     gene            694..1227
                     /locus_tag="VHK28_07565"
     CDS             694..1227
                     /locus_tag="VHK28_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142110.1"
                     /translation="MSRYLRGGLVATAAAALLTTALLTGAFAAPPDDLAAARAATARF
                     NSLKQAEKAGYALPPEGPLSQCIESLDPVNEPGAMGFHLINGALLDGVVDPTKPEALV
                     YAPDKNGKLRLVALEYVVFKSDWQGEHDPMLFGEMFMSTDAPNRYEIPAFYALHAWVW
                     EDNPAGTFAGFNPNVSC"
     gene            1322..1534
                     /locus_tag="VHK28_07570"
     CDS             1322..1534
                     /locus_tag="VHK28_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142111.1"
                     /translation="MTDQRRENRTEADDQPTEPIDEESYGASASESGGVYNEDGPTAA
                     NVSGDTMGTRDEAPPGEEPESDPYSG"
     gene            complement(1623..>1843)
                     /locus_tag="VHK28_07575"
     CDS             complement(1623..>1843)
                     /locus_tag="VHK28_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX2142112.1"
                     /translation="ALQHAREVDDREALFVVLLPDSGRNYLGKLYDDAWLREKGLLGV
                     DEEVTAYDWRATRPEVVLRGDQVPRGAG"
BASE COUNT          316 a          675 c          601 g          251 t
ORIGIN      
        1 atgctggccg acagtgaccg gaccgccgag ctgcaggacg cgcacgaccg gatgctcgcg
       61 ctcggcgccc acgcggtggc ccgcaaggtg gccggccagc tgcgccgtgt gggcggcgca
      121 gtccgccgcg gcccgcggac ggcgacgcgc gggaaccccg cccgcctgac cgaacgacag
      181 atggaggtgg cgcggctggt cgcccagggc cgctccaacc gggacatcgc agcccatctg
      241 gtcctgaccg agaagaccgt cgggcaccat gtatcggccg tgctgtcaaa gctcgacgtg
      301 caacgccgag ccgaagtcgc cggggcactc tctgaattcg cgaccagcca aagataggga
      361 agccaattcc ctaacttggg gcatctcccc gatgccgccg cacccgggcc gctcccatcc
      421 tcctggcgaa aggaggacgc acatgacgct cttcatggac gttcacagca tcgacggcgg
      481 ggtggcggcc agggatgtcg cccaggctca catcgccgat ctcaagaccc aggaccgcta
      541 cggcgttcgc tatctgcgct actgggtcaa cgagggcgac ggcaaggtct tctgcctggt
      601 cgacgccccc gatgccgatg ccgccaagcg cgtgcatcgc gaggcgcacg gcctcgtggc
      661 cgacgccatc tttccggtag tggagggaga ctgatgtccc gataccttcg cggcggcctg
      721 gtcgccaccg cagccgcggc gctgctgaca acggcgctgc tgaccggtgc ctttgccgca
      781 ccgcccgatg acctggccgc agcacgagcg gccaccgccc gcttcaacag cctcaagcag
      841 gccgagaagg ccggctacgc cctgccgccg gaggggccgc tgagccagtg catcgagtcg
      901 ctcgacccgg tgaacgagcc cggcgccatg ggcttccacc tcatcaacgg cgcgctgctc
      961 gacggcgtgg tggaccccac caagcccgag gcgctggtct atgccccgga caagaacggc
     1021 aagctacgcc tggtcgcgct cgagtacgtc gtattcaaga gcgactggca gggcgagcac
     1081 gacccgatgc tcttcggcga gatgttcatg tccaccgacg ctcccaaccg gtacgagatc
     1141 ccggccttct acgccctgca tgcctgggta tgggaggaca acccggccgg caccttcgcc
     1201 ggcttcaatc cgaacgtcag ctgctgagga cttacggtcc gcgcaattgc ctgagcggag
     1261 ccgcccggtg atgggcggct ccgccgcatg tctggcacgc cgagtgctgc cttccccgca
     1321 gatgaccgat caacgccgcg agaaccgcac cgaggccgac gaccagccca ccgagccgat
     1381 cgacgaggag tcgtacggcg cttccgcttc agagtccggc ggcgtctaca acgaggacgg
     1441 gccaacggcc gccaacgtga gcggcgacac catgggcacc cgggacgagg cgcctccggg
     1501 cgaggagccg gagagcgacc cctactcggg ctgaggtcgc ggagccgtca ccagcccatg
     1561 agtccaagat cgtcacccat accaccagcc cgtggtgaaa tcgcccagct agcgccaacc
     1621 gctcagcccg cgccgcgggg gacctggtca cctcgcagca ccacctccgg gcgcgtggca
     1681 cgccagtcgt aggcggtcac ctcctcgtcg acgcccagca ggcccttctc gcgcagccag
     1741 gcgtcgtcgt acagcttgcc caggtagttg cggccgctgt ccggcaggag gacgacgaac
     1801 agcgcctcgc gatcatccac ctcgcgtgcg tgctgcagcg ccg
//
LOCUS       DATACA010000367         8999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1091655, whole genome shotgun sequence.
ACCESSION   DATACA010000367 DATACA010000000
VERSION     DATACA010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091655"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1671)
                     /locus_tag="VHK28_07580"
     CDS             complement(361..1671)
                     /locus_tag="VHK28_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX2142113.1"
                     /translation="MSVFGLGYVGSVSAACLADRGIDVIGVDVSPIKVDTINAGRSPI
                     VEHGLAELIERGVTSGRLRATQDAGEAIRASDASLICVGTPSNENGSLDLTAVARVIE
                     TIGRTLVDGARPHVVIVRSTMLPGSTEERLVPILEAAAGRRLGDGLSICYNPEFLREG
                     SSLRDFESPPYTIIGGDDPSATDLARSLYASIDAPLHVVPIRVAEMLKYASNGFHALK
                     VAFANELGVLCKQQGVDSAALMDIFVADTKLNISPAYLRPGFAFGGSCLPKDLRAVLH
                     RARALDLSLPVLEAVLPSNRSHAERAYEIVRATGSRRIGILGLSFKAGTDDLRESPLV
                     GLVERLIGRGYDVRVYDRDVSLANVHGTNRAFIEHEIPHIMTILTDDLDEVLEHAEVV
                     VVGNASPDHGAALSRLRPDQRVIDLVRIAGREAVDPSCYEGIAW"
     gene            complement(1683..2747)
                     /locus_tag="VHK28_07585"
     CDS             complement(1683..2747)
                     /locus_tag="VHK28_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2142114.1"
                     /translation="MSSDHPDRSVDADIGRGMVLVWAPRYLGTRSAWLANQLGIADVR
                     FLAPTRARGLRAAPLKYPRQFVSTVLALARHRPRVVLVQSPPSMASWTVAVYAAFGGA
                     AFVIDAHSDAFERRLWTRPVWLNRAVVRRAAAAIVTTGHWAKRIEALGGQPLVIPAVP
                     TALEVGAPPPLDEGFNVAVVNTWAPDEPLEAVLEAAAQVPDATFHVTGNDARVADLRH
                     PVPPNVRFTGFLPEPAYHGLLANAHAVMCLTTRDHTMQNGAAEAMYLGTPIITSHWQI
                     LRDYFSKGAEHVDNTPGGIAAGVRAVMRDTDRYHAEVRELRDERREQWRRDREELVSR
                     LEGQLKAVGGRTVVGRAGGA"
     gene            complement(2744..3964)
                     /locus_tag="VHK28_07590"
     CDS             complement(2744..3964)
                     /locus_tag="VHK28_07590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2142115.1"
                     /translation="MRLTYLHGRYPVLTETFIDREIQGLLDRGIDLKVISIRPTDDVL
                     SPTQRNLKERVTYLLPASLPRIVVAHAWALLRRPRTYLWTLAWLLTRDHGAGPRHRTA
                     LHFGAGVYAAWVLRRRAGVHIHAHFVDRAATVALVASRLLGTTYSVTAHAREIYVDAV
                     LLAERIGEAAFAVTCTEYNRQYLEAELGARATSRLIRLYHGISLAAYAAIDGRQLADP
                     PLLLSVAQLWERKGLRYLVEACAQLRDRGLQFRCEIVGDGPQRAELQELIDRHQLGGV
                     VTLAGSLPHPDVVERYRRAAVFVLPCVVTDDGDRDGIPNVILEALAASVPVVSTMVSG
                     IPEVVRDGETGRAVPERDAAAVADAIVAVLDDPKAAALMAERGRRLVEDEFDVQRNVD
                     RLLERFAAVTGTGA"
     gene            complement(3991..5223)
                     /locus_tag="VHK28_07595"
     CDS             complement(3991..5223)
                     /locus_tag="VHK28_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2142116.1"
                     /translation="MSDLNVAGSGDLRTVLHVVGNLDRGGAQEVVATLARELPAHGWR
                     AVVVTLRDGPLRAEIEARGGIVEVVAGRRHSILSPLAAARELRRIRRDLGALVHQHRA
                     LVVQTHLLAGLDFLVLSLRSRRGQPAVLWTVHNARLELRPDQLPTGQRALLAPKRVAY
                     RGAYRLGGRAVDAFVAVSDEVAASVRTAYRPPRRRLATIPNGVDVDRYGYEVDRAAVR
                     RSLGLPAESRLVIVVAKLFRQKGHDVLLAALRSRPLPDDIGVLFAGEGPERRALEASI
                     SAMGLDGRVLLLGERPDVADLLAASDLFVLPSLWEGLPMALLEAMASGLPVVATDVAG
                     SRQVVVDGESGRLVPPADPEALRTAIDELLANPVLLSTMGRAARQRVVASFSSATQAA
                     QHAALYAQCLAQRGDPRA"
     gene            complement(5220..6197)
                     /locus_tag="VHK28_07600"
     CDS             complement(5220..6197)
                     /locus_tag="VHK28_07600"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142117.1"
                     /translation="MTRAPVFVGGLDRTGTSLMYALLASHPDMAMSRRTNWWTYFYGR
                     FGVLADDRNLDRCLATMARYRRHRKLEPDFERLRADFVAGKRSYGRLFALLKEQHAER
                     LGRPRWGDKSLHTERYADIVYSEFPDARILHMVRDPRDRYASVIKRWKRVRGGVGAGT
                     AAWLSSVRLAERNLRAHGERYRIVRYEELVSEPEAMVRDLCGFLELPFVPAMLRLEGD
                     AVFGESMNSSYGAIPKGTITAASVGRYRGVMRPHEIAFMQWRAGREMERHGYAPDDVE
                     LDGGAGRLHYLTRAAVHSGMMVGWQARERIYDLVGRDPSAHTLVNQELQ"
     gene            complement(6194..7075)
                     /locus_tag="VHK28_07605"
     CDS             complement(6194..7075)
                     /locus_tag="VHK28_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142118.1"
                     /translation="MNRSEPDPRPVFLTGLDRTGKTPFRRALLSHSSVWLSRRTALWS
                     FHHGRYGDLAHGASLDRCLRALLASPAVAQLVPDPAELVNALRSGVPTYGRLFGLILG
                     WHAASLGKPRWGEQDAELELAPARVFADYPDATVIHFIRDPRFRLAGVVRDSGRRPGD
                     TRVASGGWARSVRRAEAWQARFPGRYLMVRAEDFGDPPADSLAHLAERLSDGRPLRSR
                     LVEPTAEGLPSLPQTDVAFIEEHVGDLMERHGYRRMAPRLSAPARLRYRLVELPAGEL
                     RLAVRRARARTSSGAGR"
     gene            complement(7072..8082)
                     /locus_tag="VHK28_07610"
     CDS             complement(7072..8082)
                     /locus_tag="VHK28_07610"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX2142119.1"
                     /translation="MPTNAPIFIGGPDRCGKTLVAAILASHPRIAISPVGSNLWPFFY
                     RRFGDLRDDRNLERCIAAMVRYKHVAFLQPDVDRLREEMRSGERTYPRLFALVHEQYA
                     HREGKARWGDQTGLVERWADEIFASYPGVRMIHMLRDPRDRYHASLEMWPSGRLRAGG
                     ATARWRLSAELAMRNERKHGDRYRVLQYETLVRQPERTVRELCEFLDEEFVPSMLGLG
                     AMPGVRSKMQAGLATIKPEAAGEAGHGSAIISPAFIGTYRGRIRADEQAFLERHSRRW
                     MEWLGYGPEHGSRAVRRTPGYWLRTEPANVVRMLGWRWREAAAYRAPRWLGRRIRPGM
                     LR"
     gene            complement(8086..>8999)
                     /locus_tag="VHK28_07615"
     CDS             complement(8086..>8999)
                     /locus_tag="VHK28_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX2142120.1"
                     /translation="SGRSDVVSLSTDRGTIIIKRYRASVDDPAIAGEHSVLLELGRRC
                     FPAPRLVGGPSGETLVRHAGERYAAFEALEGYQPAHALLSLPGAARRLARDAGRSLAA
                     LHSALDRFAPSGTNPDGLSWPDGGRVRPVDWYLERLSAATGGRGLDPDEQRRAGERLA
                     ALETELPARLPRRAVIHGDYGPYNLLVRPGEPLVVTDFELARLDWSLTDLATALPRFA
                     ANRFGLSHRRALAFLDGYTAVIPLSEEERRLIPQVLEYLSLRRVAVCVGRWMSTGEQR
                     WLDEAVAKVRQARMLRDGRHPLARLLD"
BASE COUNT         1410 a         3173 c         3153 g         1263 t
ORIGIN      
        1 gagtcgggct ctcgcccttc aaggacacgg gcatcgcctt gtaacgcatt gtagggacct
       61 cgcgaagggt actcgggatg tccggcctac gtgctgctca accgaccggc atgtgtgcga
      121 ggcaccctaa tgaagtgcct cgcaccaggt cgactagcga atatggatgc cgcagccatc
      181 gcgatgaccg ctcgggacgc accgcatccg gacgatcgga cctcgccaga ccatacctct
      241 ccgcatcgga ccctaccgtg agggtcctga ccgagtcgga cccgcctggt acctgaggtc
      301 gagatatgcc gagccttcgc cggcccggaa gtggtgacag tcggcaccgg cctaagcccg
      361 ctaccaggcg atcccctcgt agcaggacgg atcgaccgcc tcccggccgg cgatgcgcac
      421 caggtcgatg acgcgctgat ctggccgcag gcggctcagg gcggcgccgt ggtcgggcga
      481 ggcgttgccg accacgacga cctcggcgtg ctccagcacc tcatccaggt cgtcggtcag
      541 gatggtcatg atgtggggaa tctcgtgttc gatgaacgcc cggttggtgc cgtgcacgtt
      601 cgccagggaa acgtctcggt catagacgcg aacgtcatag ccgcgaccga tgaggcgctc
      661 caccaggccg acgagcgggc tctctcgaag gtcatcggtc cccgccttga agctcaggcc
      721 aagaatgccg atgcgacggc ttccggtggc gcgcacgatc tcgtacgccc gctcggcgtg
      781 cgagcggttg ctgggcagca ccgcctccag gaccggcagg gacaggtcca gggcacgggc
      841 ccgatggagc acggcgcgca ggtccttggg aaggcacgag ccgccgaagg cgaagccagg
      901 tcgcaggtag gcgggcgaaa tgttgagctt ggtgtcggcg acgaagatgt ccatcagcgc
      961 ggcgctgtcc acgccctgct gcttgcacag cacgccgagc tcgttggcga aggccacctt
     1021 cagcgcatgg aatccgttgc ttgcgtactt gagcatctcg gccacccgga tgggcacgac
     1081 gtgcagcggg gcgtcgatgc tggcgtagag gctccgcgcc aggtcggtgg cgctcgggtc
     1141 atcgccaccg atgatcgtgt acggcgggct ctcgaagtcg cgcagcgagc tcccctcgcg
     1201 caggaactcg gggttgtagc agatggacag accgtcaccc aggcgccgcc ccgcggccgc
     1261 ctccaagatc ggcaccagcc gctcctccgt gctccccggc agcatggtgc tgcgcacgat
     1321 gaccacgtgc gggcgggcac catcgaccag cgtccggccg atcgtctcga tgacgcgcgc
     1381 gaccgcggtg aggtccaggc tcccgttctc gttggacggg gtccccacgc agatgagcga
     1441 ggcgtcgctc gcccggattg cctcgccggc gtcctgagtg gcccgcagcc gcccgctggt
     1501 cacaccgcgc tcgatcagct ccgcgagccc gtgctcgacg atggggctcc ggccggcgtt
     1561 gatggtgtcg actttgatcg ggctcacgtc gacgccgatg acgtcgatgc cgcgatcggc
     1621 caggcaagcc gccgaaacgg atccgacgta gcccaggccg aacacgctca cggtagtcaa
     1681 attcaggctc ctcccgcgcg gcccacgaca gtgcggccgc caaccgcttt cagctgtcct
     1741 tcgagacgcg acacgagctc ctcgcggtcg cgccgccact gctcgcgacg ctcgtcgcgg
     1801 agctcgcgga cctcggcatg gtaccggtcg gtgtcgcgca tcaccgcccg cactccggcg
     1861 gcgattcctc ccggcgtgtt gtccacgtgc tcggcgccct tcgagaagta gtcgcgcagg
     1921 atctgccagt ggctggtgat gatgggcgta ccgaggtaca tcgcctcggc agcaccgttc
     1981 tgcatggtgt ggtcgcgggt ggtcaggcac atcaccgcgt gggcgttggc cagcaggccg
     2041 tggtaggcgg gttccggcag gaacccggtg aagcgcacgt tgggcgggac cggatggcgc
     2101 aggtcggcaa cacgagcgtc gttgcccgtg acatggaacg tggcgtcggg aacctgcgcc
     2161 gccgcctcca gcacggcttc cagcggctcg tccggcgccc aggtgttgac gacggccacg
     2221 ttgaagcctt cgtccagcgg tggtggtgcg ccgacctcca gcgccgtcgg aacggccggg
     2281 atgacgaggg gctggccgcc gagggcctcg atgcgcttgg cccagtgtcc ggtggtcacg
     2341 atggcggctg ccgcccgccg gaccacagcc cggttgagcc agaccggcct ggtccatagc
     2401 cggcgctcga aggcgtcgct gtgcgcgtcg atgacgaagg cggcgccgcc gaacgcggcg
     2461 tagacggcga ctgtccagga cgccatgctg ggcggactct ggaccaggac aacccgcgga
     2521 cggtgccgcg ccagcgcgag cacagtgctc acgaactggc gggggtactt cagaggcgcc
     2581 gcacgcaggc cccgcgcccg ggtcggcgcc aggaagcgga cgtcggcaat tcccagctga
     2641 ttcgcgagcc aggcgctgcg ggtgccgagg tagcggggcg cccagaccag gaccatgcca
     2701 cggccgatgt cggcatccac cgaccgatcc gggtggtcgg agctcacgct cccgtcccgg
     2761 tgacggcggc gaagcgttcg agcaggcggt cgacgttgcg ctgcacgtcg aactcgtctt
     2821 cgaccagccg ccggccgcgc tcggccatca gggccgccgc cttggggtcg tcaagcaccg
     2881 cgacgatggc atcggccacg gcggcggcgt cgcgttcggg gaccgccctg ccggtctccc
     2941 cgtcgcgcac cacctccggg atgcccgaaa ccatggtgga gaccacaggc accgaggccg
     3001 ctagtgcctc caggatcacg ttggggattc catcgcggtc gccgtcatcg gtgaccacgc
     3061 agggcaggac gaacacggcc gcccggcggt agcgctccac gacgtccggg tgcggcaggg
     3121 agccggccag ggtcaccact ccgccgagct ggtgtcgatc gatcaactcc tgcagctcgg
     3181 cccgctgcgg cccgtcaccg acgatctcgc agcggaattg gaggccccga tcgcgaagct
     3241 gcgcgcaggc ctcgaccagg tagcgcaggc cctttcgctc ccagagctgg gcgaccgaca
     3301 ggagcagtgg cgggtcggcc agctggcgcc catcgatggc ggcgtacgcg gccaggctga
     3361 tgccgtggta gagacggatc agtcggctcg tcgcgcgggc accgagctcg gcttccaggt
     3421 actgccggtt gtactcggta cacgtcaccg cgaacgcggc ctccccaatc cgctcggcta
     3481 atagcaccgc gtccacgtag atctcacggg cgtgggcggt aacgctgtac gtcgttccca
     3541 gcagtcgcga ggcgaccagc gcgacagtgg ccgcgcgatc cacgaaatgg gcatggatgt
     3601 ggaccccggc gcgccggcgc aggacccagg cggcgtatac gcctgcgccg aagtggaggg
     3661 cggtccggtg ccgcggccct gcgccgtgat ccctggtcag caaccaggca agcgtccaga
     3721 ggtaggtgcg cggccggcgg agcagcgccc aggcgtgggc gaccacgatg cgcggcagcg
     3781 atgcgggaag caggtaggtg acccgctcct tcaggttccg ctgcgtcggc gacaggacgt
     3841 cgtcggtcgg ccggatggaa atgaccttca ggtcgatccc gcggtccagc agtccctgga
     3901 tctcgcggtc gataaacgtc tcggtgagga ccgggtagcg cccatggagg tacgtgaggc
     3961 gcatcggtgc gccagcgggc tggtcggcat tcacgcgcga gggtctcccc gttgtgcaag
     4021 gcattgggcg tagagggcag catgctgcgc cgcctgcgtg gcgctgctga aggatgccac
     4081 gacccgctgc cgagcggctc tacccatggt gctcaggagc accggattcg cgaggagctc
     4141 atcgatggcc gtgcgcagcg cttcggggtc ggcgggcggg acgagtcgtc ccgactctcc
     4201 atccaccacc acctgacggc tgccggccac gtcggtggca accaccggca gaccggatgc
     4261 catggcctcc agcagcgcca tcggcaggcc ttcccacaga gaagggagga cgaagagatc
     4321 gctggccgcc agcaggtcgg cgacgtccgg ccgctcgccc agcagcagga cacggccgtc
     4381 caggcccatg gcgctgatgc tcgcttccag ggcgcgccgc tcggggcctt cgccggcgaa
     4441 cagcaccccg atgtcgtcag gcaggggtcg ggagcgcagg gccgccagca acacgtcgtg
     4501 gcccttctgc cggaacagtt tggcgaccac gatcaccagg cggctctccg cgggcagtcc
     4561 gagactgcgg cgcacggcgg cgcggtccac ctcgtagccg tagcgatcga cgtcgacccc
     4621 gttgggaatg gtggccaggc ggcggcgggg cgggcggtac gccgttcgca ccgaggccgc
     4681 cacctcatcg gaaacggcca cgaaggcgtc cacggcgcgc cctccgagcc gataggcgcc
     4741 gcggtacgcc acgcgcttcg gagcgagaag cgctcgctgt cccgtcggca gctggtcggg
     4801 ccgcagctcg aggcgggcgt tgtgcacggt ccacagcacc gccggctggc cgcgccggga
     4861 gcgaagggag agcaccagga agtcgaggcc cgccagcagg tgcgtctgga ccacgagggc
     4921 tcggtgctgg tggaccagcg cgccgaggtc acgccgaatg cgccgcagct cccgcgcggc
     4981 tgccagcggt gagaggatgg agtgcctccg cccggcgacc acctccacga tacccccacg
     5041 cgcctctatc tccgctcgca acggcccatc gcggagcgtc accaccaccg cccgccagcc
     5101 gtgggcgggc agctcccggg ccagcgtggc aaccacctcc tgcgcgcccc cgcgatccag
     5161 gttgcccacg acatggagca cggtccgcag gtcgcccgac ccggcgacgt tgagatcgct
     5221 cactgcagct cctggttgac cagggtgtgt gccgaggggt cgcgaccgac caggtcgtat
     5281 atgcgctctc gcgcctgcca gccaaccatc atccccgagt ggaccgcggc cctcgtcagg
     5341 tagtgaagcc ggcccgcgcc gccgtcgagc tccacatcat ccggggcgta cccgtgccgt
     5401 tccatctcgc ggccggcgcg ccactgcatg aacgcgatct cgtgcggccg catcacgccg
     5461 cggtagcggc cgacggacgc cgcggtgatg gtccccttcg ggatggcgcc gtacgagctg
     5521 ttcatggact cgccgaagac ggcgtctccc tccaggcgca gcatggccgg cacgaacggc
     5581 agctcaagga agccgcacag gtcgcggacc atggcctccg gctcggacac cagctcctcg
     5641 tagcgcacga tgcggtagcg ctcgccgtgc gcgcgcaggt tccgctccgc caggcgcacc
     5701 gaggacagcc aggcggcggt gccggctccg acgccgccgc gcacccgctt ccagcgcttg
     5761 atgaccgatg cgtaccggtc ccgcgggtcg cggaccatgt gcaggatccg agcatccggg
     5821 aactcgctgt acacgatgtc cgcgtatcgc tcggtatgca gcgacttgtc accccagcgc
     5881 gggcgaccca gccgctcggc gtgctgttcc ttgagcagcg cgaagaggcg gccgtaggaa
     5941 cgcttccccg cgacgaagtc ggcgcgcaag cgctcgaaat cgggctccag cttccggtgt
     6001 cgccggtagc gcgccatggt ggccaggcag cgatcaaggt tgcggtcgtc ggcgaggacc
     6061 ccgaaccggc cgtagaagta cgtccaccag ttcgtccgac gcgacatcgc catgtcggga
     6121 tgggaggcca gcaatgcgta catcaggctg gtgcctgtcc gatccaaacc cccgacgaag
     6181 accggggcgc gggtcatcgg cccgctccac tagaagtgcg cgcgcgagcg cggcgaaccg
     6241 cgagccgcag ctcaccggcc ggcagttcga ccagccgata ccggaggcgt gccggggcgc
     6301 tgagccgcgg cgccatacgc cggtatccat gcctctccat caggtcgccc acgtgctcct
     6361 cgatgaatgc gacgtcggtc tggggcagtg atggtaggcc ctcggccgtc ggctcgacca
     6421 atcgggaccg aagcggccga ccgtcactca ggcgctcggc gagatgggcc agagagtccg
     6481 ccggcggatc accgaagtcc tcggcgcgca ccatgaggta gcggcccggg aaccgtgcct
     6541 gccaggcctc ggctcggcga accgagcgcg cccagccacc gctggccacc cgggtgtccc
     6601 ccggtcgacg gccgctgtcg cgcaccacgc cggcaagccg gaagcgtgga tcacggatga
     6661 agtggatcac ggtcgcatcc gggtagtcgg cgaacacgcg cgccggcgcg agctcgagct
     6721 ccgcgtcctg ctcgccccag cggggcttgc ctaggctcgc cgcgtgccac cccaggatca
     6781 ggccgaagag ccggccgtag gtggggacgc cggagcggag ggcgttcacc agctcagccg
     6841 gatccggcac cagctgtgcc actgccgggg aggcgaggag ggcccgcagg catcggtcga
     6901 ggctggcgcc atgggccagg tcaccgtagc gcccgtgatg gaaagaccac agcgccgttc
     6961 ggcggctgag ccacaccgac gagtgcgaca gcagcgcgcg acggaacggg gtcttaccgg
     7021 tgcgatccag gccggtgagg aagaccggcc gtgggtcagg ctcggaacgg ttcaccgcag
     7081 catccctggc cggatgcgcc ggccgagcca ccgcggagcg cgatatgcgg ctgcctcgcg
     7141 ccatcgccag cccagcatgc ggaccacgtt cgccggctcc gttcgcaacc agtaccccgg
     7201 cgtgcggcgc acggctcgcg agccatgctc cggcccatac ccgagccact ccatccaccg
     7261 tctcgaatgc cgctcgagga acgcctgctc gtcggcgcga atgcgtccgc ggtacgtccc
     7321 gatgaaggcc ggggagatga tggcggaccc atggcccgcc tcccccgccg cctcgggctt
     7381 gatggtggcc aggcctgcct gcatcttgga ccgcaccccc ggcatggcgc ccagcccgag
     7441 catggatggc acgaattcct cgtcgaggaa ctcgcacagc tcgcgcaccg tccgctccgg
     7501 ttggcggacc agtgtctcgt actgcaggac gcgataccgg tcgccgtgct tgcgctcatt
     7561 tcgcatcgcc agctcggcgg agaggcgcca gcgcgccgtg gcgccaccgg cgcgcagtcg
     7621 tccagagggc cacatctcga gcgatgcgtg gtagcgatcc cgcgggtcgc gcagcatgtg
     7681 gatcatgcgc acccccgggt aggacgcgaa gatctcgtcg gcccagcgct cgaccagtcc
     7741 ggtctgatcg ccccaccggg ccttgccctc gcgatgcgcg tactgctcgt gcaccagcgc
     7801 gaaaagcctc ggataggtcc gctcgccgct gcgcatctcc tcccgcagcc ggtccacgtc
     7861 gggctggagg aaggccacgt gcttgtagcg gaccatggcc gcgatgcagc gctctaggtt
     7921 gcgatcgtcc cgcaggtcgc cgaagcggcg gtagaagaac ggccacaggt tcgagcccac
     7981 cggcgagatg gcgatcctcg gatgcgaagc caggatcgcc gccaccaggg tcttgccgca
     8041 gcggtccggc cctccgatga agatgggcgc attcgtgggc atcggtcagt ccaaaagccg
     8101 cgccagcgga tgccggccgt cgcgcagcat tcgtgcctgg cggaccttcg cgaccgcctc
     8161 gtccaaccag cgctgctcgc cggtcgacat ccagcggccg acacacaccg ccacccgacg
     8221 caggctcagg tactcgagga cctggggtat cagcctgcgc tcctcctcgg acagggggat
     8281 cacggccgtg taaccgtcca ggaacgccag cgcccgccgg tgcgataagc cgaagcggtt
     8341 agcggcgaac cgaggcagcg ccgtggcgag gtcggtgagc gaccagtcca ggcgagccag
     8401 ctcgaagtcg gtcacaacca atggctcgcc gggtcgcacc agcaggttgt atggcccata
     8461 gtcgccgtgg atcaccgcgc gtcgcggcaa tcgggccggg agctccgtct ccagggccgc
     8521 gagccgctcg cccgctcgcc gctgctcgtc tggatcaagc ccgcggccgc ctgtggcggc
     8581 ggacagccgt tccaggtacc agtcgaccgg gcgcacgcgt ccgccgtcgg gccaggacag
     8641 gccatccggg ttcgtcccgg acggcgcgaa ccggtccagc gccgagtgga gggccgccag
     8701 ggagcggccg gcgtcccgcg ccaggcggcg cgcggcaccg ggcagcgaca ggagtgcgtg
     8761 tgccggctga tagccctcga gtgcctcgaa ggcagcgtat cgctcgccgg catgccgcac
     8821 cagcgtctcg cccgacggtc cgccgacgag acgcggggct ggaaagcatc gccgccccag
     8881 ctcgagcagg acactgtgct cgccggcgat ggccgggtcg tcgaccgacg cgcggtagcg
     8941 cttgatgatg atcgtcccgc ggtcagttga gagtgacacc acatcgctac gcccggatg
//
LOCUS       DATACA010000368         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2617736, whole genome shotgun sequence.
ACCESSION   DATACA010000368 DATACA010000000
VERSION     DATACA010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2617736"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VHK28_07620"
     CDS             <1..198
                     /locus_tag="VHK28_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX2142121.1"
                     /translation="GTLYAVLARLEERGLIEPLPAEDRRRPYRLTSVGADALEVQLAE
                     LSRFVQLGRRRLRQAEVPGNG"
     gene            191..1039
                     /locus_tag="VHK28_07625"
     CDS             191..1039
                     /locus_tag="VHK28_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX2142122.1"
                     /translation="MADRLASRFLVRCYPRAWRDRYGAEMLALLAERGIGLRDALDVL
                     AAALDARLRPAVQQPAPQAILAARPDPAPVRSPAAFGIVAGPSRNVIGRRAFMRRMLG
                     VGAGLLALEFAGGTLSFLWPQIREGLGAKFRLGTLPDILTAQPSFANGWPYPYAPARI
                     FLVNQPAAMELALGREASVTEPAADELLALWRKCPHLGCLVPEPCESVTRYRCRCHGS
                     TYNILGEKLEKGPAERGMDRFPVTIENGVVVVDTSQRIAGAPNLGPERLEFRDAHPWE
                     ATCAEA"
     gene            complement(1050..>1767)
                     /locus_tag="VHK28_07630"
     CDS             complement(1050..>1767)
                     /locus_tag="VHK28_07630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2142123.1"
                     /translation="FDAHALLQAFRRRARSLGVDYVTGEVTSLERDGSRITAARLANG
                     GSIGLDWVVDAAGPRAADVAALAGLDLPVRPRKRLVFHFECPEPLGPAPLTVNPGGAY
                     FRPEGPAWIGGFSPREGETDPDAVDLQVDRSRFDERLWPELAHRVPAFARLRLLDAWA
                     GLYEVNTLDHNAVVGPHTEVGNFLFANGFSGHGLQQAPAVGRGLAEWICSGRWETIDL
                     SPLGYQRITRNQPLRELNVV"
BASE COUNT          231 a          646 c          642 g          248 t
ORIGIN      
        1 gggaccctgt acgccgtgct ggccaggctc gaggagcgtg ggctgatcga gccgctgccg
       61 gcggaggacc ggcggcgtcc ctaccggctc acctccgtcg gggccgatgc gctcgaggtc
      121 cagctcgccg agctgagccg cttcgtgcag ctcggtcgac gccggctgcg gcaggccgag
      181 gtgccgggca atggctgacc gcctcgccag ccgcttcctc gtgcgctgct atccgcgagc
      241 ctggcgggat cgctacgggg ccgagatgct tgcgctgctg gccgagcgtg gcatcggcct
      301 gcgggatgcc ctcgacgtgc tggccgctgc tctcgacgcg cgcctgcgtc cggccgtcca
      361 gcagccagcg ccgcaggcca tcctggccgc ccgaccggat cccgcgcccg tgcgatcgcc
      421 ggccgccttc ggcatcgtcg ccggcccctc gcgcaacgtc atcggtcgtc gagccttcat
      481 gcgccgcatg ctcggggtgg gcgccgggtt gctggccctg gagttcgccg gcggcaccct
      541 gagcttcctg tggccgcaga tccgcgaggg gcttggcgcc aagttccggc tcggtaccct
      601 gcccgatatc ctcaccgccc agccatcctt cgccaacggc tggccgtacc cgtacgcgcc
      661 ggcgcgcatc ttccttgtca accagcctgc cgccatggag ctggcgctgg ggcgcgaggc
      721 atcggtgacc gaaccggccg ccgatgagtt gctggcgctg tggcgcaagt gcccgcacct
      781 cggttgcctg gtgcccgagc catgcgagtc ggtgacccgc taccggtgcc gctgccacgg
      841 ctccacctac aacatcctgg gcgagaaact ggagaaggga ccggccgagc gggggatgga
      901 ccgcttcccg gtgacgatcg agaacggagt ggtcgtggtc gataccagcc agcgaatcgc
      961 cggcgcgccg aatctcgggc cggagcgcct ggagttccgc gacgcgcacc cctgggaggc
     1021 gacctgcgcc gaggcctgag gccggcgcac tagaccacgt tcagctcgcg cagcggctgg
     1081 ttgcgggtga tccgctggta gccgagcggc gagaggtcga tcgtctccca gcgtcctgag
     1141 cagatccact ccgccagccc gcggcccacg gccggcgcct gctgcagtcc gtggccggag
     1201 aagccgtttg cgaacaggaa gttgccgacc tcggtgtgcg gccccaccac cgcgttgtgg
     1261 tccagcgtgt tcacctcgta gagaccggcc caggcgtcga gcaggcgcag ccgcgcgaag
     1321 gccgggacgc ggtgggcgag ctccggccac aggcgctcgt cgaacctcga gcggtccacc
     1381 tgaaggtcca cggcgtccgg atccgtctcg ccctcgcgcg gcgagaagcc gccgatccag
     1441 gccggtccct ccggccggaa gtaggcgccg cccgggttga cggtcagcgg ggcaggtccc
     1501 agcggctccg ggcactcgaa gtggaagacg aggcgcttgc gcggtcgcac gggcaggtcc
     1561 aggccggcca gggcagccac gtccgccgcg cgcggtccgg ccgcgtcgac cacccaatcc
     1621 aggccgatgg agccgccgtt cgccagccgc gccgccgtga tccgggaccc gtcgcgctcg
     1681 agtgacgtga cctcgccggt gacgtagtcc acgccgagcg agcgggcgcg ccgccggaag
     1741 gcctggagga gggcgtgggc gtcgaac
//
LOCUS       DATACA010000369         9056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1091877, whole genome shotgun sequence.
ACCESSION   DATACA010000369 DATACA010000000
VERSION     DATACA010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091877"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VHK28_07635"
     CDS             complement(<1..808)
                     /locus_tag="VHK28_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2142124.1"
                     /translation="MTVNARPQVVVITGASAGVGRALARLYADRGAWVGLIARGRAGL
                     TAARQEVEALGGRGLELPLDVADADAVEAAAERVERELGPIDVWINNAMASVFSPVRE
                     MKVEEFRRVTEVTYLGAVHGSLSALRRMQSRDRGTIVLVGSALAYRGIPLQSAYCAAK
                     HAMQGFHDSLRTELMHDGSNVKVTMVQLPAVNTPQFGWVLSRLPNRAQPVPPIFQPEV
                     IAEGIAWAADNPDATRELNIGFPTLKAVWGNKLAPTVADRVLASSGYTSQQ"
     gene            complement(840..2300)
                     /locus_tag="VHK28_07640"
     CDS             complement(840..2300)
                     /locus_tag="VHK28_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142125.1"
                     /translation="MHRLRVPGGAAVLLPAAVLAGLLLGQLSADPAAGVTFSNSPFTD
                     EAWNVMAARNAVLLGDWAAGNWALHLVQLPFTLLEAAGFALLGVGILQARLVATLCTL
                     ASVALIGLVVGRRLGAGAGVVAGAALGSCALLLFYGRLVYIENLVLLGLAGGIAALAW
                     RPGAASWAGAALGGVLLAVAIGSKPLALFSVIGILAGTALGAWSRAWTLRVLVASAVI
                     GLAGIAWLLLIGLPNSRQIGWVREIWAQQSAPADLGQMIGRVLSYPTAGDRAVLLSLP
                     LSLAGIAGLGCAVLGWRALDRGQRALAGAAMGWLVLGLGVLLVASYRPSRYALPYLPA
                     LAVLAGFAVPVAARLLARVPTAVARGALAGAVAGLLIGPGLASYAGWAARTDHRAAAL
                     QAEIAGLMAQDPRPIEGGLAPFFAMRVPVPIYVRWSSSRVNSGDLYAEEDVRWMLLTG
                     TQHPLWADAHPEAWEGRRELGCYAWGEGRHCLLRFP"
     gene            complement(2300..4261)
                     /locus_tag="VHK28_07645"
     CDS             complement(2300..4261)
                     /locus_tag="VHK28_07645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX2142126.1"
                     /translation="MFTERGAGYRPYLDGLRAIAVAGVIVYHLNPGWLAGGYLGVDLF
                     FVLSGYLITTLLVNEHAKTGRIDLPAFWSRRVRRLMPALMLLLVAVAIGVLLSGDELA
                     AASARGDMLATLFYVANWHFIWTDQSYFAGYVAASPVRHAWSLAIEEQFYLAWPLVVG
                     LVLSRLGGRRTLVVLAALASVASVAAMWILFGTAADPSRAYYGTDARIYQLLIGAILA
                     VALQGRWRATVLAAARPMAVPALLAVGGMMVLLTDDSALYYRGGGPAFAVASAVLIAG
                     LEGGSRLARPLSIAPMVVIGLVSYGLYLWHWPVILFVGQAVGATSGLPPALLAVALTA
                     VLAAISYVAVERPIRRGGRIGPVSLQPRRLLGLVPATSAVVAGVILLGTSFGSTPPWA
                     STSGEVPPGATVTIGEEGEPVVGVVGDSVMVSALPGLRVEAQSRGWTLVEAAFAGCPV
                     GYEPLYDAQGVESSFSERCLSVRPAHDALIDARPDVVIWHDLQSVLSRRSESGDLLPA
                     GTSSWAEDLLAEWQAVLDRLTAGGSQVLIIMPPRRSIDEAGCGTAVNAARCREVQAQD
                     ELMRATTMAFWERIDGRDGVHLVDVDRIVCPDGYPCPTALEGISIRRGPWDQTHFSEE
                     GAAWFARRLFDEALRPAAGGAPTLDAARP"
     gene            4352..4428
                     /locus_tag="VHK28_07650"
     tRNA            4352..4428
                     /locus_tag="VHK28_07650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4386..4388,aa:Pro,seq:tgg)
     gene            4466..4999
                     /locus_tag="VHK28_07655"
     CDS             4466..4999
                     /locus_tag="VHK28_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX2142127.1"
                     /translation="MIMDVAEVRAVLARTPSVLRLLVDGLSEPWLDSRPADGEWSAHQ
                     VICHLAYVEETDWLVRARMILEVGTGTAFPPVEHGDQSDRYAGLSTDEVLSRFTAERE
                     ANLSAMGELDLSIASLERRGLHPTLGEVTLQQLMATWLVHDHNHVAQLQAALASHYVA
                     EVGPWRSFLGILDRVER"
     gene            5016..5972
                     /locus_tag="VHK28_07660"
     CDS             5016..5972
                     /locus_tag="VHK28_07660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX2142128.1"
                     /translation="MLTLIAAFGLALVAAWLLTPVVRDAAHGAGLLDEPEDRKVHLVP
                     IPRLGGVAIGGAFFLGMAAALVVARATGAPLATETGQLPGILVGGALIAGVGLLDDLQ
                     GMRARVKLAAQLVVALVAYGLGLSIDQLSGPWGTLELGAWALPLTVAWIVAVVNAVNL
                     VDGVDGLASGVALTALAAYFGIGLVHGGIEPVLPVVAAGAGGVIGFLRYNLHPASIIM
                     GDSGSMLIGFLLAAVGISLTQAEPAGAAPWIPALVVALPLADMAWAILRRVVARAPVF
                     APDKRHIHHQMMAAGLSQRTTMLLLTAASAVFGGVAFLLVLA"
     gene            complement(5969..7465)
                     /locus_tag="VHK28_07665"
     CDS             complement(5969..7465)
                     /locus_tag="VHK28_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2142129.1"
                     /translation="MTQPARHRHPALAATLSFLLPGLGQAYAGQPLLAAMLAIPVLLL
                     IAGGLLVALVWGDQVRNSLFSATFLTGLLILDLALMVWRVFAIAHVGFARPVPVAVDG
                     AASGPTGGPALGHPSARAGIGRAASIGLVGLLIVATVVMHTWAGMVVTRMESTLDDVF
                     GGIPWSGGRVPGGGDDERPLNEPEYAWDGTERISFLLLGIDAAPGREHALTDTILVVS
                     VDPAARTAVMVSVPRDTGFVPLPDRTVYPDGVYPGKINQLSSEAAENVEAWCPDLPPD
                     AGAACGVRTLERAVSLYLGIPIQYYAQVDLAGFQSLVDAVGGLDLCLQGTLVDPQYLG
                     VGTADRGITLPAGCTHYDGARALAYARIRQGWMEMSDGTRVQQDDFMRAERQQRVLLE
                     FRRELARLDLIFELPPVLEAIGRTVVTDFPREQAGDLATLLPLITGPDIERVVLGLPE
                     FVDPPVDPTVNYLLIPRREQVRAEMERLFGAENLEGWYLTTDAPGPPG"
     gene            7720..>9056
                     /locus_tag="VHK28_07670"
     CDS             7720..>9056
                     /locus_tag="VHK28_07670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX2142130.1"
                     /translation="MEIELIFIIVTGIIAAMLGLGALVAAIRSARRAGSVVDLDDPDR
                     EVAEAYDSDPVRAQEGVDATYGSAVRILWWISIAAVLIGVGLSDAFDASQPGIYLVGG
                     VAVGVVVLLHDVLPNRLRSGTVAVLEVLVAIGLTTLLLTLTGYGGSPFAFGYHLAAVA
                     VALALGSSAGLGVAAVATLAYLGVLAIDPSLPDYQGADLLRFGLSIGAIWLLAYVAGV
                     FASGERRMRATVLQLSRTDTLTSLFNRSQLFPTLEQEVQRTRRSERGFCLLMIDLDGL
                     KAVNDSFGHHRGDEVLRSLGNVIRRSIRSVDTAYRYGGDEFLVLLPETDFAGAFVVAE
                     KIRAGAEAVGLAAGGGDLLTSVSIGLVSCPEDGTSAEELMIAADRAMYQAKSLGKNKV
                     SGNPRPPRLPYRPEPPDGEPEPPLSEVPPIQMPRVGGAAVPGLGRAEQAIEEPP"
BASE COUNT         1329 a         3275 c         3253 g         1199 t
ORIGIN      
        1 tctgctggga ggtgtagccc gaggacgcga gcacccggtc ggcgaccgtg ggcgccagct
       61 tgttgcccca caccgccttg agggtgggga agccgatgtt caactcacgg gtcgcgtccg
      121 ggttgtcggc ggcccaggcg atcccctcgg cgatgacctc cggctggaag atgggcggca
      181 ccggctgcgc ccggttcggg aggcgggaca gcacccagcc gaactgcggc gtgttcacgg
      241 cgggcagctg gaccatggtg accttcacgt tgctgccgtc gtgcatcagc tcggtgcgca
      301 gcgagtcgtg gaagccctgc atggcgtgct tggcggcaca gtatgccgac tgcagcggga
      361 tcccgcggta ggcgagggcg gagccgacca gcacgattgt gccgcggtca cgcgactgca
      421 tgcgacgcag cgcggacagc gagccatgca cggcgcccag gtaggtgacc tcggtcaccc
      481 ggcggaactc ctcgaccttc atctcgcgca cgggcgagaa gaccgatgcc atggcgttgt
      541 tgatccacac gtcgatcggt cccagctcgc gctccacccg ctcggccgcg gcctcgacgg
      601 catcggcatc ggccacgtcc agcggaagct ccagcccgcg cccgccgagc gcctccacct
      661 cctgccgggc cgccgtcagg ccggcgcgcc cgcgcgcgat cagccccacc caggcgccgc
      721 gatccgcgta gagcctggcc agcgcacggc cgaccccggc cgacgctccg gtgatgacca
      781 cgacctgcgg cctggcattg acggtcacga ccgaccgcgg cctgcaggtt cgctgccagc
      841 tagggaaagc gcagcaggca gtgcctcccc tccccccacg cgtagcagcc cagctcgcgg
      901 cgaccctccc aggcttccgg gtgcgcgtcc gcccagagcg ggtgctgggt cccggtgagc
      961 agcatccagc gcacgtcctc ctcggcgtag aggtcgccgc tgttcacgcg gctagatgac
     1021 cagcgcacgt agatcggcac cggcacccgc atggcgaaga agggagccag cccgccctcg
     1081 atcggtcggg ggtcctgggc catcagcccg gcgatctcgg cctgcagcgc cgccgcccgg
     1141 tggtcggtcc gcgccgccca accggcgtag ctcgccagcc cgggacctat gaggaggccg
     1201 gccacggcgc cggccagcgc cccccgcgcc acggccgtcg gaacccgcgc cagcaggcgg
     1261 gccgcgacgg gcactgcgaa tccggccagg acggccaggg cgggcaggta gggcagcgcg
     1321 tagcggctgg ggcggtagga cgccaccagc agcacgccga gccccaggac cagccagccc
     1381 atggccgcgc cggccagtgc ccgctgcccg cggtccagcg cgcgccagcc caacacggcg
     1441 cagcccagcc cggcgatgcc ggccagcgat aacggcagcg acagcagcac ggcccgatcg
     1501 cccgcggtcg ggtagctcag cacccggccg atcatctgac caaggtcggc cggcgccgac
     1561 tgctgcgccc agatctcgcg cacccagccg atctggcgcg agttcggcag gccgatgagc
     1621 agcagccagg cgatcccggc cagtccgatg accgccgatg cgaccagcac ccgaagcgtc
     1681 caggcccggc tccacgcccc cagcgcggtg ccggccagga tgccgatcac cgagaagagc
     1741 gccagcggct tgctgccgat ggccaccgcc agcagcaccc cgcccagcgc cgcgccggcc
     1801 cagctcgcgg ctccgggacg ccaggcaagg gccgcgatgc cgccggcaag ccccagcagc
     1861 accaggttct cgatgtacac cagccgcccg tagaagagca gcagcgcgca cgacccgagg
     1921 gcggccccgg ccaccactcc ggcgccggcc cccagccggc gtccgaccac caggccgatg
     1981 agtgccacgc tggccagcgt gcacagcgtg gccaccagcc gggcctgcag gatgccgacg
     2041 cccagcaggg cgaagccggc cgcctcgagc agcgtgaacg gcagctgcac caggtgcagg
     2101 gcccagttgc cggcagccca gtcgccgagg agcacggcgt tgcgggccgc catcacgttc
     2161 cacgcctcgt cggtgaacgg cgagttgctg aaggtgacgc ccgcggccgg gtctgccgac
     2221 agctgtccca gcagcagccc ggccaggacg gcggccggca gcagcacggc ggcgccgccc
     2281 ggaacccgca gccggtgcat cagggacggg cggcgtcgag ggtcggcgcg ccgccggctg
     2341 cggggcgcag ggcctcatcg aacagccgcc gcgcgaacca cgccgctccc tcctcgctga
     2401 agtgcgtctg gtcccacggc ccgcgccgga tggagattcc ctccagggcg gtcgggcacg
     2461 ggtagccatc cgggcacacg attcgatcga cgtccaccag gtggaccccg tcgcggccat
     2521 cgatccgctc ccagaaggcc attgtggtcg cccgcatcag ctcatcctgg gcctgcacct
     2581 ctcggcaccg cgccgcgttg acggcagtcc cgcagccggc ctcgtcgatg gagcggcgtg
     2641 gcggcatgat gatcaggacc tgactgccac cggccgtgag acggtcgagc accgcctgcc
     2701 actccgccag caggtcctcc gcccagctcg aggtcccggc cggcagcagg tcgccggact
     2761 ccgagcggcg cgagagcacc gactgcaggt cgtgccagat cacgacgtcg ggccgggcat
     2821 cgatcagtgc atcgtgcgcc gggcgcacgg agaggcagcg ctcgctgaag ctggactcga
     2881 ctccctgcgc gtcgtacagc ggctcgtaac caaccgggca gccggcgaag gcggcctcga
     2941 ccagggtcca gccgcgcgac tgcgcctcga cccgcaggcc gggcagggca gagaccatga
     3001 cagagtcccc gaccacgccc accaccggct cgccctcctc accgatggtt acggtggcgc
     3061 cggggggaac ctctcccgag gtcgatgccc agggaggagt gctgccgaag ctcgtcccca
     3121 gcaggatgac cccggccacc accgccgagg tggccgggac caaccccagc agacggcggg
     3181 gttggaggct gaccggaccg atgcgtccgc cgcgccggat cggccgctcg accgcgacgt
     3241 aggagatcgc ggccagcacc gcggtcaggg ccacggccag cagcgcgggc ggtagcccgc
     3301 tggtcgcccc cacagcctgg ccgacgaaga ggatgaccgg ccagtgccac aggtagagcc
     3361 cgtacgaaac cagtccaatc actaccatgg gcgcgatcga cagcggccgc gccagccggc
     3421 tgccgccctc cagcccggcg atcaggaccg ccgaggcgac cgcgaaggct ggtccgccac
     3481 cacggtagta cagcgcggaa tcatcggtca gcaggaccat catgccgccc accgccagca
     3541 gggcgggcac agccatcgga cgcgcggcgg ccagcaccgt cgcccgccag cggccctgga
     3601 gcgcgacggc caggatggcg ccgatcagca gctggtagat gcgcgcatcg gtcccgtagt
     3661 aggcgcgtga cgggtccgcc gcggtgccga acaggatcca catggcggcc accgaggcca
     3721 ccgatgcgag ggcagccagg accaccagcg tgcgcctgcc tccgaggcgg ctcagcacca
     3781 ggccgaccac cagcggccag gccaggtaga actgctcctc gatggccagc gaccaggcat
     3841 ggcgcaccgg cgaggcggcc acgtacccgg cgaagtagga ctggtcggtc cagatgaaat
     3901 gccagttggc gacgtagaag agcgtggcca gcatgtcgcc ccgggcggag gccgcggcca
     3961 gctcgtcgcc ggacagcagc acgccgatcg ccaccgccac cagcagcagc atcagggccg
     4021 gcatcaggcg ccggacgcgg cgcgaccaga acgcgggaag gtcgatgcgc ccggtcttcg
     4081 cgtgctcgtt caccagcagg gtggtgatca ggtagccgct gagcacgaag aagaggtcaa
     4141 ctcccaggta gccgccggca agccagcccg ggttgaggtg atagacgatc acccctgcga
     4201 ccgcgatcgc ccgcagcccg tccagatagg gccggtatcc ggccccgcgc tcagtgaaca
     4261 tcccgtccgc tcaacatgcc ggacgcaggg tacccggccg gccgcggctt cgccgccccc
     4321 gggtggctca actagaatgg cacgcggaac ccggggcgta gcgcagcctg gtagcgcacc
     4381 tgctttggga gcaggaggtc gccggttcga atccggccgc cccgaccatc ccacgatacc
     4441 ctgggcgcga ggtgccgagc agccgatgat catggacgtc gccgaggtgc gcgccgtgct
     4501 ggcgcgaacg ccatcggtcc tgcggctcct ggtcgacggt ctgtcggagc cgtggctgga
     4561 ctcgcgaccg gcggacggcg agtggagcgc gcaccaggtg atctgccacc tggcgtacgt
     4621 ggaggagacc gactggctgg tccgcgcgcg gatgatcctg gaggttggga ccggcaccgc
     4681 cttcccgccc gtcgagcacg gcgaccaatc cgaccgctac gccggcctgt cgaccgatga
     4741 ggtcctgtcc cgcttcacgg cggaacgtga ggcgaacctg tcggcgatgg gggagctgga
     4801 cctgtcgatc gcctcgctgg agcggcgcgg gctgcacccg accctcggcg aggtgaccct
     4861 ccagcagctg atggccacct ggctggtcca cgaccacaac cacgtggcgc agctccaggc
     4921 ggcgctggcg tcccactacg tcgccgaggt cgggccgtgg cgctccttcc tggggatcct
     4981 ggaccgggtc gagcgttgag gcgggtgccg aacgggtgct gaccctgatc gcggcgttcg
     5041 gactggccct ggtggcggcg tggctgctga cgcccgtggt gcgggatgcc gcccacggcg
     5101 ccgggctgct ggacgagccg gaggatcgga aggtccacct ggtgccgatt cccaggctgg
     5161 gcggggtggc aatcggcggc gccttcttcc tcggcatggc cgcggcgctg gtcgtggcgc
     5221 gcgccaccgg ggcgccgctg gccaccgaga ccgggcagct acccggcatc ctggtcggtg
     5281 gcgcgctcat cgccggggtg gggctgctcg acgacctgca gggcatgcgc gcgcgggtca
     5341 agctggcggc ccagctggtg gtggcgctgg tggcctacgg gctgggcctc tccatcgacc
     5401 agctcagtgg cccgtggggc accctggagc tcggggcctg ggcgctgccg ctcaccgtgg
     5461 cctggatcgt ggcggtggtc aacgccgtga acctggtgga cggcgtggat gggctggcct
     5521 caggcgtggc gctgaccgcc ctggcggcct acttcggtat cggtctggtc cacgggggga
     5581 tcgagccggt cctgccggtg gtggcggccg gcgccggcgg ggtcatcggc ttcctgcgct
     5641 acaacctgca tccggccagc atcatcatgg gtgactcggg atcgatgctg atcggcttcc
     5701 tgctggcggc ggttggcatc agcctcaccc aggccgaacc ggccggagcg gcaccctgga
     5761 tcccggctct ggtggtcgcc cttccgctgg ccgacatggc gtgggccatc ctgcgccggg
     5821 tggtggcccg ggcgcccgtc ttcgccccgg acaagcgcca catccatcac cagatgatgg
     5881 cggccggcct gtcgcagcgc accacgatgc tgctcctcac ggccgcttcg gccgtgttcg
     5941 gcggcgtggc cttcctgctg gtgctcgcct agcccggcgg gccgggcgca tcggtcgtca
     6001 ggtaccagcc ctccaggttt tccgcgccga acaggcgctc catctcggcg cgaacctgct
     6061 cgcggcgggg gatgaggagg tagttcacgg tcgggtcgac cggtggatcg acgaactcgg
     6121 gcaggccgag caccacgcgc tcgatgtccg ggccggtgat cagcggcagc agcgtggcga
     6181 ggtcgccggc ctgctcgcgt gggaagtcgg tgaccaccgt cctgccgatt gcctccagga
     6241 ccggcggcag ctcgaagatg aggtcgaggc gcgccagctc gcggcggaac tcgagcagga
     6301 cccgctgctg gcgctcggcg cgcatgaagt cgtcctgctg gacccgcgtg ccgtcgctca
     6361 tctccatcca gccctgccga atgcgcgcgt aggccagcgc gcgtgcgccg tcgtagtgcg
     6421 tgcagcccgc cggcagggtg atcccgcggt cggcggtgcc gacgcccagg tactgcgggt
     6481 ccaccagggt gccctgcagg cagaggtcca gtccacccac cgcgtcgacc aggctctgga
     6541 agccggccag gtccacctgc gcgtagtact ggatggggat gcccaggtac aggctgacgg
     6601 cacgctccag cgtgcgcacg ccgcatgctg ccccggcatc cggtggaagg tcggggcacc
     6661 aggcctcgac gttttcggcg gcctcgctgg agagctggtt gatctttccc gggtagaccc
     6721 cgtccgggta gaccgtgcgg tcaggcaggg ggacgaaacc cgtgtcgcgc ggcacggaga
     6781 ccatgaccgc cgtgcgggcc gccggatcta cgctgaccac caggatggtg tcggtcaggg
     6841 cgtgctcacg ccccggcgcg gcgtcgatgc ccagcagcag gaagctgatc cgctcggtgc
     6901 cgtcccacgc gtactccggc tcgttgagtg ggcgctcatc gtcgccgcca cccggcaccc
     6961 ggccgccgga ccacgggatg ccgccgaaga cgtcatccag ggtgctttcc atccgggtca
     7021 ccaccatccc ggcccatgtg tgcatgacca cggtcgccac gatcagcagg ccgaccagcc
     7081 cgatgctggc cgcgcgcccg atgcccgccc tggcagaagg atggccgaga gcggggccgc
     7141 cggttgggcc gcttgccgca ccgtccacgg cgacgggaac gggacgcgcg aagccgacgt
     7201 gagcgatggc gaacacgcgc cacaccatga gcgccaggtc gaggatcagc agtcccgtca
     7261 ggaaggtcgc ggagaacagg ctgttgcgca cctggtcgcc ccacaccagc gccaccagca
     7321 gcccgccggc gatcagcagc agcaccggga tcgccagcat cgcggccagc agcggctggc
     7381 cggcgtaggc ctgccccagg ccggggagca ggaaggagag cgtcgccgcc agtgcggggt
     7441 gacggtggcg cgcgggctgg gtcatggcac ctctgcgggc tgccggcgcc acgccgggcg
     7501 ccacgggcgg ccgattctag catcggggag ctcgctctcc gggtgagcga ccgctcggca
     7561 cgaagtggtg acggcacgcg ccatgcgatg tcggtacggt tgggttaggt ggtaccggta
     7621 cctttgcgcc atgactctcg gcgcccgtag actaccgatg gtcgtgcccg gactcgccta
     7681 cgcccggtgt gccggtttga ccgctggagt gggtggccct tggagatcga gctaatcttc
     7741 atcatcgtca ccgggatcat cgccgccatg ctcgggctcg gtgcactggt ggccgcgatc
     7801 cggtcagcgc ggcgcgcggg ctccgtggtg gacctcgacg atcccgaccg cgaagtggct
     7861 gaggcctacg acagcgaccc cgtgcgggcg caggagggcg tggacgccac gtacggctcg
     7921 gcagtccgca tcctgtggtg gatctcgatc gcggcggtcc tgatcggcgt tgggctctcg
     7981 gacgcgttcg atgcctccca gccggggatc tacctggtcg gcggcgttgc ggtgggggtc
     8041 gtggtcctgc tgcacgatgt gctcccgaac cgcctccgct ccggcacggt ggccgtcttg
     8101 gaggtgctgg tcgccatcgg gctgaccaca ttgctgctga cgctgactgg gtacggcggt
     8161 tcacccttcg ccttcggcta ccacctcgcc gcggtggcgg tggcgctggc actggggagc
     8221 agcgcggggc tcggcgtggc ggccgttgcc accctggcct acctcggcgt cctggccatc
     8281 gacccctcgt tgccggacta ccagggcgcg gacctcctgc gcttcggcct ctccattggt
     8341 gccatctggc tgctggccta cgttgccggc gtcttcgcct cgggtgagcg ccggatgcgc
     8401 gcgaccgtcc ttcagctgtc acggaccgat acgctgacca gcctcttcaa ccgcagccag
     8461 ctgttcccca ccctggagca ggaggtgcag cgcacccggc gcagcgagcg cggcttctgc
     8521 ctgctgatga tcgacctgga cggcctcaaa gcggtgaatg actcgttcgg ccaccaccga
     8581 ggggacgagg tgctgcgatc gctgggcaac gtcatccgtc gctcgatccg cagcgtcgac
     8641 accgcgtatc gctacggtgg tgacgagttc ctggtgctcc tgccggagac cgatttcgcc
     8701 ggcgcgttcg tggtcgccga gaagatccgg gcaggcgccg aggcggtcgg cctggcggcc
     8761 ggcgggggag acctgctgac cagcgtcagc atcggcctgg tgtcctgtcc ggaggacgga
     8821 accagcgccg aggagctgat gatcgcggcc gaccgggcga tgtaccaggc caagtcgctc
     8881 ggcaagaaca aggtgagcgg caacccgcgc ccgccgcgcc tgccgtaccg ccccgagccg
     8941 ccggacggag agccggagcc gccgctctcg gaggtgcccc ccatccagat gccgcgcgtc
     9001 ggaggggcgg ccgtgcccgg gctgggccgg gcggagcagg cgatcgagga gccgcc
//
LOCUS       DATACA010000370         5002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_609939, whole genome shotgun sequence.
ACCESSION   DATACA010000370 DATACA010000000
VERSION     DATACA010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609939"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHK28_07675"
     CDS             complement(<1..587)
                     /locus_tag="VHK28_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142131.1"
                     /translation="MDRPEPLSRYTRATAHLLAAALAPLAVAVLGLFAPLSFAELSGA
                     SWVVPAISGSAAVVLGSAMAVVLALALVGARPPAMLHAGALGALTAGLAVVAVTGALA
                     GSGPEPSRNVAAAGAIAAAMLLAARLVPAQGEDGLGARVAVAVGVFVAAELALAATLL
                     MALPPELVAWAYLSAAALALAASLPAISRAEALTGG"
     gene            705..1115
                     /locus_tag="VHK28_07680"
     CDS             705..1115
                     /locus_tag="VHK28_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142132.1"
                     /translation="MEERATRPTAPGRRTVMSRRRFLEQTDRLIADGEQLLATPDWNL
                     FRAWLLNSDELLERVWGRMDRYHLAWLNVGRGSAPPGSALDESGTARFVREVAGGKLA
                     VLRTMREAVATRGWGLLSDDEEEPQVKPHDEEER"
     gene            1112..1849
                     /locus_tag="VHK28_07685"
     CDS             1112..1849
                     /locus_tag="VHK28_07685"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2142133.1"
                     /translation="MTRHRPDDEIGGLAAVLAHPDDESFGCAGALALAHDAGRSVRLL
                     VVTRGEAGSVPEGVDPADFATTREDELRCAASKIGFTEVTVLEGYPDGGVADLPFAQL
                     VDEIATWLGDRQPDAVITFGAHGITGHPDHVVVGSATRWAVERLAESGQAPHAVYVIA
                     PVFGGGARRYDLSAEEGAATHRIDITAVAGRKIAALACHASQPDAAQEAVDQQTVLDR
                     DGVLYEGYTRVRPPVPAPHPKFDTRLV"
     gene            1897..2187
                     /locus_tag="VHK28_07690"
     CDS             1897..2187
                     /locus_tag="VHK28_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX2142134.1"
                     /translation="MTIDSNLGKVDEVMTALYDIHDPEIGMSIVDLDLIKNVEIGTDG
                     TPTEIKMVLTTPFCPWAGELIQTVKEKTEEVVGPPVKVTLLADRWEPPPGLF"
     gene            complement(2135..2674)
                     /locus_tag="VHK28_07695"
     CDS             complement(2135..2674)
                     /locus_tag="VHK28_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142135.1"
                     /translation="MSERMDGGTAETVGRKRILVINDTKEILELFRDLLENEMGHDVT
                     LMSYAPDELARIVEAQPDLVILDFVIGDREAVGWQLLQKLRMHPATARIPVVACTAAI
                     QLVRESQGYLLQQGVEVVLKPFTLEQLEHAVHGALRLDGSTILTKQPATDGQRDRSTR
                     RPPRRARGAAPSDRQAGSP"
     gene            2737..3303
                     /locus_tag="VHK28_07700"
     CDS             2737..3303
                     /locus_tag="VHK28_07700"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2142136.1"
                     /translation="MRIYTRKGDTGTTGLLFGGDRISKADLRADAYGTTDEAVSALGL
                     ARAAIGASTDRSEAHLTELTVRLQRELFVVGAELATHADRRGRLTPGTSLVTAEMVSA
                     LEAEIDALESGMAMPTEFVVPGESLPGAGFDLARTTVRRAERRAVALTEAGELPDSQV
                     VPYLNRLADLLWVMARAADGGFVPVRER"
     gene            3547..4917
                     /locus_tag="VHK28_07705"
     CDS             3547..4917
                     /locus_tag="VHK28_07705"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX2142137.1"
                     /translation="MTLSATKHRMFINGEWFDAISGETADVINPATEEVIAQVPRGDV
                     ADVDRAVQAARDAFESWSLTTPAERSAMLHRFADALAADAETLSSLEQQNVGKPKGTA
                     DFDVEFTIDNVRFFAGAARVVEGKAAGEYVSTHTSMIRREPAGVVGQVAPWNYPLMMA
                     IWKLCPALAAGCTVVLKPSSLTPLSALRIGELAADIFPKGVLNVITGPGASVGARLVT
                     HPQVDMVSLTGDTATGREIAAAAAQTVKRVHLELGGKAPVLVFDDADPAAVAEGVKVA
                     GFFNSGQDCTAATRIIAGPRIYDSLMSELVPAIESVKVGNPADDPELDMGPLVSRSQQ
                     ERVVGFVDRARGYGATVAAGGGTNGERGYFYSPTIVTDVAQDAEIVQQEVFGPVVTVQ
                     RFADDQEAIRWANGVSYGLAASVWTRDVKRALNAARRLRYGTVWINDHLTIASGSATV
                     GTGATW"
BASE COUNT          754 a         1753 c         1802 g          693 t
ORIGIN      
        1 ccgccggtca gcgcctcggc gcgggagatg gccggcagcg aggcggccag ggcgagagcc
       61 gcggcgctga ggtatgccca tgccaccagc tcgggcggca gcgccatcag cagcgtggcc
      121 gccagggcca gctccgccgc cacgaagacg ccgacggcca cagccacccg cgcccccagc
      181 ccatcctcgc cctgggcggg caccaggcgc gcggcgagca gcatggcggc cgcgatcgcg
      241 cctgcggctg ccacgttccg gcttggctcc ggcccggacc cggccagggc cccggtgacg
      301 gccaccaccg ccaacccggc cgtcagggca cccagcgccc ccgcgtgcag catggccggc
      361 ggacgtgcac cgaccagcgc cagggccagc accaccgcca tggccgatcc caggaccacg
      421 gccgcgctgc cggagatggc gggcacgacc caggacgcgc cggaaagctc cgcgaacgag
      481 aggggcgcga acaggcccag gacggcgacg gccagcggcg ccagggcggc cgccagcagg
      541 tgggccgtcg ctctggtgta gcgggagagt ggctcgggac ggtccacgcg ggcaccggtg
      601 cggacgtgcc agggcagtct tggatggccg gcagccagcg gcaatagtac gaaggcaccg
      661 cgcggtgatc ggcgggcgca cgcacgctgc cctatgctcg cggcatggag gagcgggcaa
      721 cgcgccccac ggcacccggc cggcggaccg tcatgtcccg ccgccgcttc ctggagcaga
      781 ccgaccggct gatcgccgac ggcgaacaac tgctggcgac gccggactgg aatctgttcc
      841 gcgcctggct gctcaactcc gacgagctcc tcgaacgggt gtggggccgt atggaccgat
      901 accacctggc ctggctcaac gtcggccgcg gctcggcgcc gcccggctcg gcgctcgacg
      961 agtcggggac tgcccgcttc gtacgcgagg tcgccggcgg caagctggcc gtgctgcgta
     1021 ccatgaggga ggcggtcgcg acgcgtggct ggggcctgct gagcgatgac gaggaggagc
     1081 cccaggtgaa gccccacgac gaggaggaac ggtgacccgc catcgcccgg acgatgagat
     1141 cggcggcctg gcggccgtgc ttgcccaccc ggacgacgag agcttcggct gtgccggggc
     1201 gctggcgctg gcccacgatg ccggacgcag cgtcaggctc ctggtcgtga cgcgtggcga
     1261 ggcgggcagc gtgcccgagg gcgtcgaccc ggccgacttc gccactaccc gcgaggacga
     1321 gctgcgctgt gctgcctcca agatcggctt caccgaggtg acggtgctcg agggctaccc
     1381 ggacggtggg gtggccgacc ttcccttcgc gcagctggtg gacgagatcg ccacctggct
     1441 gggcgatcgg cagccggacg cggtgatcac cttcggggcg cacggcatca ccggtcaccc
     1501 ggaccacgtg gtggtcggct cggccacgcg ctgggctgtc gagcgcctgg cggagagcgg
     1561 gcaggcgccg catgccgtgt acgtcatcgc ccccgtgttc ggtggcggcg cccgccgcta
     1621 cgacctctcg gcggaggaag gggcggccac ccaccgaatc gacatcaccg cggtggcggg
     1681 tcgcaagatc gcggcgctgg cctgccatgc ctcgcagccg gatgcggccc aggaggccgt
     1741 cgaccagcag accgtgctgg accgcgacgg agtcctgtac gagggctaca cccgcgtccg
     1801 gccaccggtc ccggcgcccc acccgaagtt cgacacgcga ttggtctagg gccgcctgac
     1861 ggccatcggt ccaatccttg gtagactggt cgggatatga cgatcgactc caacctcggc
     1921 aaggtggacg aggtgatgac cgccctctac gacatccacg atcccgagat cgggatgagc
     1981 atcgtggacc tggacctgat caagaacgtc gagatcggga cggacggcac gcccaccgag
     2041 atcaagatgg tgctgaccac gcccttctgc ccctgggccg gggagctgat tcagacggtc
     2101 aaggagaaga cggaggaggt cgtgggaccg cccgtcaagg tgaccctgct tgccgatcgc
     2161 tgggagccgc cccccgggct cttctaggag gacggcgcgt gctgcggtcg cgctggccat
     2221 cggtggcggg ctgcttggtc aggatcgtgg acccgtccag tcgcagggcg ccgtgcaccg
     2281 cgtgctccag ctgctccagc gtgaacggct tgaggaccac ctccaccccc tgctggagca
     2341 ggtagccctg gctctctcgc accagctgaa tggcggcggt gcaggccacc accgggatcc
     2401 tggcggtcgc cgggtgcatc cgcagcttct gcagcagctg ccagccgacg gcctcgcggt
     2461 cgccgatcac gaagtccagg atgaccaggt ccggctgtgc ctcgacgatt cgcgcgagct
     2521 cgtccggcgc gtagctcatc agggtcacgt cgtggcccat ctcattctcg agcaggtcgc
     2581 ggaagagctc gaggatctcc ttggtgtcgt tgatgaccag gatccgcttg cgaccgaccg
     2641 tctccgcggt gcctccgtcc atccgttcgg acatggggcc gcgtggaatg cacgtcgcgt
     2701 gccgggggcg ggcggatggg cggtaccatc ggtctcgtgc gcatctatac ccggaagggc
     2761 gacaccggca ccaccggcct gctgttcggc ggcgaccgga tcagcaaggc cgacctgcgg
     2821 gcggatgcct acggcacgac cgatgaggcg gtctcggcac tggggctggc gcgggccgcc
     2881 atcggcgcca gcacggatcg ctccgaggcg cacctcacag agctcaccgt acgcctgcag
     2941 cgcgagctgt tcgtggttgg ggcggagctg gcgacgcacg ctgatcgtcg cgggcggttg
     3001 acgcccggca ccagcctggt gacggccgag atggtctcgg ccctggaagc cgagatcgac
     3061 gcgctcgagt cggggatggc catgccgacc gagttcgtgg tgcccggcga gtcgctgccc
     3121 ggtgccggct tcgacctggc gcgcaccacg gtgcgccgtg ccgagcggcg agcggtggcg
     3181 ctcaccgagg ccggcgagct gcccgactcc caggtcgtcc cgtacctgaa ccgcctggcc
     3241 gacctgctgt gggtcatggc ccgcgccgcc gatggcggct tcgtccccgt tcgcgagcga
     3301 tgagcgcccg attcggccaa cggccgcccc agttcgcgtc cacgaagtgt tccggtaatc
     3361 tgaacacttt cgcgcaagtg ctccgtccac ccgacctgct gcggaatcgg ccgacaagcg
     3421 gccatgggga ctaccgaatc cgccatgacc ggcgttagaa tcgaccgata ccggcgcgcc
     3481 ggggcctgct gtggcccgcg caatgggtcc accatgcctc tgtgcctgca ctcccgagag
     3541 gttcacatga cgctctccgc caccaagcac cgaatgttca tcaacggcga atggttcgac
     3601 gcgatcagcg gcgagaccgc ggacgtgatc aaccccgcca ctgaagaggt gatcgcgcag
     3661 gtcccgcgcg gcgacgtcgc ggacgtcgac cgggccgtgc aggccgcgcg cgacgcgttc
     3721 gagtcgtggt cgctgacaac gccggccgag cgcagcgcga tgctgcatcg cttcgccgac
     3781 gcccttgccg ccgacgccga gacgctctcc tcgctcgagc agcagaacgt gggcaagccg
     3841 aagggcaccg ccgactttga cgtggagttc accatcgata acgtgcgctt cttcgcgggt
     3901 gccgcccggg tggtggaggg caaggccgcc ggcgagtacg tgagcaccca caccagcatg
     3961 atccggcgtg agccggccgg ggtggtgggg caagtagcgc cctggaacta cccgctgatg
     4021 atggccatct ggaagctgtg cccggcgctg gccgccggct gcacggtggt gctcaagccg
     4081 tccagcctga cgcccctgtc cgcgctgcgc atcggggagc tggcggccga catcttcccc
     4141 aagggcgtgc tcaacgtgat caccggcccc ggcgccagcg tgggtgcgcg gctggtgacc
     4201 cacccccagg tcgacatggt cagcctcacc ggtgacacgg ccaccgggcg cgagatcgcg
     4261 gcggccgcgg cgcagaccgt gaagcgcgtc cacctcgagc tgggtggcaa ggcgccggtg
     4321 ctcgtcttcg atgacgcgga ccctgccgcc gtggccgagg gcgtcaaggt ggccggcttc
     4381 ttcaactccg ggcaggactg cacggcggcg acgcgcatca tcgccgggcc gcgcatctac
     4441 gacagcctga tgtccgagct cgtgccggcc atcgaatcgg tcaaggtcgg caacccggcc
     4501 gatgaccccg agctggacat gggcccgctg gtcagtcgct cgcagcagga gcgcgtggtc
     4561 ggcttcgtgg accgcgcccg aggctacggc gccacggtgg ccgccggtgg cgggaccaac
     4621 ggggagcgcg gctacttcta ctcgccaacc atcgtgaccg atgtcgccca ggacgccgag
     4681 atcgtgcagc aggaggtctt cggtccggtc gtcaccgtgc agcgcttcgc cgacgaccag
     4741 gaggccattc gctgggccaa cggcgtgagc tacgggctgg cggcatctgt ctggacgcgg
     4801 gacgtgaagc gggcgctcaa cgccgcgcgt cggctgcggt acgggacggt gtggatcaac
     4861 gatcacctga ccatcgcgtc cggctcagcg acggtcggga cgggcgctac ctggtgacct
     4921 ggaacgacgc gctccacatc ggttcccagg gctatgggct cctcaacgag ctcgagcggc
     4981 gcggcttcga cgtcggcgtc ga
//
LOCUS       DATACA010000371         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2698682, whole genome shotgun sequence.
ACCESSION   DATACA010000371 DATACA010000000
VERSION     DATACA010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698682"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..862)
                     /gene="arsM"
                     /locus_tag="VHK28_07710"
     CDS             complement(50..862)
                     /gene="arsM"
                     /locus_tag="VHK28_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX2142138.1"
                     /translation="MTEQIHEQVRQRYAQAAVQVMKTDAAACCTEPGIGPELYSALQR
                     DELPDGAVLASLGCGNPTAVAELNAGERVLDLGSGGGIDVLLSARRVGPTGRAIGLDM
                     TDEMLALAQRNAVEAGVTNVEFLKGHIEAIPLPADSVDVVISNCVINLAADKPSVFRE
                     MARVLRPGGRIGVSDIVADDSLSLDERAKRGSYVGCIAGALSFSEYRTGLEAVGLTDI
                     AVTPTHAVADGMHSAIIKATKPLDAAPSVLLDSRPEELALAGAGCCGGGGCC"
     gene            complement(889..1179)
                     /locus_tag="VHK28_07715"
     CDS             complement(889..1179)
                     /locus_tag="VHK28_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX2142139.1"
                     /translation="MKSADPDVQLLQALADPVRLSIVRQLAACDQVCACDFTECCAVS
                     QPTVSHHLKVLREAGVVTSERRGTYVYYALAPEFARRWAALGSSLAGLVSVA"
     gene            1347..>2059
                     /locus_tag="VHK28_07720"
     CDS             1347..>2059
                     /locus_tag="VHK28_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142140.1"
                     /translation="MTAQQLLSYLSQAIYLALFVVAAVRLFRRPSLAAFDTFLFFSVI
                     AVLLLASDAARIFGFQDNPALAILTWVGVSALPLILLRLADDFRPQPSLVMIGASLAF
                     VLVAALGVLAPQPWQPPVQLVPVGWVVVLGVYASWAFVRESRITTGLTRRRVQAVAAG
                     SFLLALVLLIAGVNVLVPAVREPLGIVSQAVGLALVSAYFVGFAPPALLQRAWQEPVL
                     RRFLAETPELVQLTSVDDIV"
BASE COUNT          284 a          737 c          687 g          351 t
ORIGIN      
        1 tgggcagcac cttcgagatc tggctgcccc gccatcagga ggcgtccgcc tagcagcagc
       61 cgccgcctcc gcagcagccg gcgccggcca atgccagctc ctcggggcgc gaatccagca
      121 agaccgatgg cgccgcgtcc agcggcttgg tcgccttgat gatcgccgag tgcatcccgt
      181 cagcgaccgc gtgggtcggc gtcaccgcga tatcggtcag tcccaccgcc tcgaggccgg
      241 tgcggtactc agagaacgac agtgctccgg cgatgcagcc cacgtacgaa ccgcgcttgg
      301 cgcgctcgtc aagcgagagg gagtcatccg ccacgatgtc gctgaccccg atccgtccgc
      361 cgggtcgaag cacgcgtgcc atctcgcgga agaccgatgg cttgtccgcc gccaggttga
      421 tgacgcagtt gctgatcacc acgtccaccg agtcggcggg cagcgggatg gcctcgatgt
      481 ggcccttgag gaactcgacg ttggtgacgc cggcctcaac cgcattccgc tgggccagag
      541 ccagcatctc gtcggtcatg tccagcccga tggcccgacc cgtcggcccc acccgccgcg
      601 ccgacagcag aacgtcgatc cctccccccg aacccagatc gaggacgcgc tcgccggcgt
      661 tcagctccgc gacggcggtc gggttgccgc agcccagcga ggccagcact gcgccgtccg
      721 gcagctcgtc gcgctgcagc gcgctgtaaa gctcggggcc gatgcccggc tccgtacagc
      781 aggcggcagc atcggtcttc atcacctgga cggcggcctg cgcgtagcgc tggcgcacct
      841 gctcgtggat ctgctcggtc atcggtcctt ctccttgaaa gtgtcgcgtc aagcgaccga
      901 caccaggcca gcgaggctgg agcccagggc cgcccagcgt cgcgcgaatt ccggtgccag
      961 cgcgtagtag acgtaggttc cccgtcgttc gctggtgacc acgccggcct cccgcagcac
     1021 cttgaggtgg tggctgacgg tgggctggct gacggcgcag cactcggtga agtcgcaggc
     1081 gcatacctgg tcgcaggcag ccagctgccg gacgatgctc aggcgcacgg gatcggcaag
     1141 ggcctggagg agctggacgt cgggatcggc tgacttcacc tcgccagcat atagatgact
     1201 gtcaatatag gcaacccgcg atcgcccgcc gagccgcggg tgcttggagg tggcgtgtag
     1261 gattagcgcc ggccacccga gcggccggca tcccgcacct gcaatcgtgc ctgcaacgcg
     1321 ggggagaagc tggagtcggc gaccaggtga cagcacagca gctgctgagc tacctcagcc
     1381 aggccatcta cctggctctc ttcgtggtcg ccgccgtgcg cctcttccgc cggccatccc
     1441 tggccgcctt tgacaccttc ctcttcttct cggtgatcgc cgtcctgctg ctggcgtcgg
     1501 atgccgcgcg catcttcggc ttccaggata acccggcgct ggccatcctg acctgggtcg
     1561 gcgtctcggc tctgcccctg atcctgctgc gcctggcaga cgacttcagg ccgcagccgt
     1621 ccctggtcat gatcggtgcg tcactggcct tcgtgctggt ggcggccctg ggtgtgctgg
     1681 ccccgcagcc ctggcagccg cccgtgcagc tcgtcccggt cggctgggtg gtcgtgctgg
     1741 gcgtgtatgc gtcgtgggcc ttcgtccgcg agagccgcat caccaccggc ctgacacggc
     1801 gccgggtgca ggccgtggcc gccggctcct tcctgctggc gctcgtgctc ctgatcgccg
     1861 gcgtgaatgt cctggttccc gccgtgcgcg agccactcgg catcgtgagc caggcggtcg
     1921 gcctcgcctt ggtgagcgcc tacttcgtgg gcttcgctcc tcccgccctg ctgcaacgag
     1981 cgtggcagga gccggtcctg cggcgcttcc tggccgagac gcccgaactc gtgcagctga
     2041 cgagcgtgga cgacatcgt
//
LOCUS       DATACA010000372         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_851750, whole genome shotgun sequence.
ACCESSION   DATACA010000372 DATACA010000000
VERSION     DATACA010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851750"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VHK28_07725"
     CDS             complement(<1..293)
                     /locus_tag="VHK28_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142141.1"
                     /translation="MPIPHPAGARPSRLLPIGLAVLLALIVAACSQNPESPSLLPSSL
                     PSPSGSSAPMETEGPSPTPAPTPPPTPTYTNPPDAELQALIPAEVGGATVTIPP"
     gene            complement(338..3727)
                     /locus_tag="VHK28_07730"
     CDS             complement(338..3727)
                     /locus_tag="VHK28_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2142142.1"
                     /translation="MPQPTRDRATEPDALLGVAQAASLLGVHPNTLRAWTDAGRLTAY
                     RINARGDRRYRRGDVERLLAEGGEVAGDEDRAHGPGERQAELAVLALLAHNGGPSSTP
                     AAVARTAAEALRTQLGAARVAVYLTREGSAGTLTLETHAGHRLPPPAVFHQTDGEAAS
                     PGGPVRLVPLHSHGERLGVIVLEDEPGGPLEAVRPSFLRTVAGAVSADLLSARAIARA
                     RREVARARALRHVTQELTGKLDLNAVLEDIVDRTRTLFEADKVGLWLVDDTEHPFKVA
                     ASRGLGEAFHDTVRGLTLSSTAVGVRAVHERKTLVVRDADRLPGIGSMQAAYRAESIR
                     TACLVPLVSNDRALGLIGLYHSRDRDWPDDELGLAQAFANQAAVAISNARLYRSVADQ
                     AARMRSIQDLSARLNRLTDVQAIADAIVAEASTLAAYHDIRVYAVDWERGDCEPVAYT
                     DRLLGEGDFREALRVPIGEGSFTGWVAEHGEPILANDALNDPRGLTIDGTDDIEESML
                     VVPMLFEGRSVGVIALSKLGKDQFTNDDLQTMTIFAGYAAQAFANAAAYERLELQSAE
                     LARQLQSQRRLLEINERLLSTLDHADVLETIADGLRAVVDYDNLSIYRTDDVNRVLQP
                     VLTRELHAEQVARYVVPFGRGLMGWAVDHREPILANDALNDPRAMQIPGTPADPEALV
                     VVPLISDGDVIGCLNISRVGGREVHFSEADFELVKLFAGQASIALRNAEAHQAVTVRA
                     DTDALTGLGNHGAFQRVLSDVLGQPEGTGGWRRTSGGAAGPNAAGPITMLMMDLDNFK
                     AYNDRLGHPAGDALLHAIGTAIYGAARTEDRVFRYGGDEFALILPGVDAPEAAAVGER
                     VRRAVGRLTADEPTAVTITIGLASYPAHASEKNDLIAAADTALYWGKQGGENRVVRAD
                     EVPREMHDLRGTLDQLARTALRHTADRAGVEGLVEAARRTAETARLGEETVRDALLAI
                     ARSLDARDATTLGHGDRVGRLARRVAEHLGCDEDLASTVELAARLHGLELTGADELDA
                     IASLREVGRIMRWQRTHGAADQAPLGAQVVAVANAYDTMLTVSGQAHDRQSALEQIRA
                     GVGSRYRREVIEALASVVAARPRRRARRRRQDEQPDIEGAA"
     gene            complement(3875..>4377)
                     /locus_tag="VHK28_07735"
     CDS             complement(3875..>4377)
                     /locus_tag="VHK28_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142143.1"
                     /translation="LHRDVEVRLPGRGWRSIERVRETRGFELWDHDGSLPLDPAAAAE
                     PLVAIPHVWRGTTAELTAPDYRASLDRLLPAGSNPLPARSTTRVVSTVDRLLVLAELR
                     RENGRLTLAPPPGGYVISALELPDAMRLLGAPRRRLLLAGAGMVAAGMLLGLVALVLA
                     LLTGLA"
BASE COUNT          585 a         1544 c         1620 g          628 t
ORIGIN      
        1 ggcgggatgg tcacggtcgc gccgcccacc tcggccggga tgagggcctg gagctcggca
       61 tccggcgggt tggtgtaggt cggggtggga ggcggcgtgg gtgccggcgt tgggctcggg
      121 ccttccgtct ccatcggcgc gctcgatccg gatggggatg gaaggctgga tgggagcaac
      181 gaggggctct ccgggttctg cgagcaggcg gccacgatca gcgccaggag gacggcgagc
      241 ccgataggga ggagtcgtga cgggcgtgcg ccagcggggt gggggatcgg catgggagcc
      301 gcgatcatag catcggcgcg gctccggccg gcggtgccta ggctgcgccc tcgatgtcgg
      361 gctgctcgtc ctgccgcctg cgtcgcgcgc ggcggcgggg tcgcgccgcg accacggagg
      421 ccagggcctc gatcacctcg cggcggtacc ggctgccgac ccctgcgcga atctgctcca
      481 gcgcggactg gcggtcgtgc gcctggccgc tcaccgtcag catggtgtca taggcgttgg
      541 ccaccgccac gacctgcgcg cccaggggcg cctggtcggc ggcgccatgg gtcctctgcc
      601 agcgcatgat gcgacccacc tcccgcaggg atgcgatggc atccagctcg tccgccccgg
      661 tcagctccag gccgtgcagt cgagcggcca gctcgacggt gctggccagg tcctcgtcgc
      721 agcccaggtg ctctgccacc cgtctggcca gccgtccaac ccgatcgccg tgtccgagcg
      781 tggtggcgtc ccgcgcgtcg agcgaacggg cgattgccag cagcgcgtcg cgcaccgtct
      841 cctccccgag gcgcgccgtc tctgcagtcc gtcgagcggc ttcgaccagg ccctccacgc
      901 ccgccctgtc ggcggtgtgg cgcagcgcgg tgcgggccag ctggtcgagc gttccccgca
      961 ggtcatgcat ctcgcgcggg acctcgtcgg cgcgcacgac ccggttctcc ccgccctgct
     1021 tgccccagta cagggccgtg tcggccgcgg cgatgaggtc gttcttctcg ctcgcgtggg
     1081 ccgggtagga ggcaaggccg atggtgatgg tgaccgccgt cggctcgtcg gccgtcagcc
     1141 gcccgacagc gcgccgaacg cgctcgccaa cggcagctgc ctctggggcg tcgactccgg
     1201 gcaggatcag cgcgaactcg tcgcccccgt agcggaagac gcggtcctcc gttcgcgctg
     1261 cgccgtagat ggcggtgccg atggcatgca gcagggcgtc cccggccggg tgacccaggc
     1321 gatcgttgta ggccttgaag ttgtccaggt ccatcatcag catcgtgatc gggccggccg
     1381 cgttcggccc cgccgcgccc ccggacgtgc ggcgccatcc gcccgtgccc tccggctgcc
     1441 cgaggacgtc cgagagaacg cgctggaatg caccgtggtt gccgaggccg gtcagcgcgt
     1501 cggtgtcggc gcggacggtg actgcctgat gggcctcggc gttgcgcagc gcgatcgacg
     1561 cctgtccggc gaacagcttg accagctcga agtccgcctc ggagaagtgg acctcgcggc
     1621 caccgacgcg ggagatgttc aggcaaccga tgacgtcgcc gtccgagatg agcggcacca
     1681 ccaccagcgc ctccgggtcg gccggtgtcc ccgggatctg catggcccgc gggtcgttca
     1741 gcgcatcgtt ggccaggatg ggctcgcggt gatcgacggc ccagcccatc aggccgcggc
     1801 cgaaggggac cacgtagcgc gccacctgct cggcgtgcag ctcacgggtc agcaccggct
     1861 gcaggacgcg attcacgtcg tccgtgcggt agatggacag gttgtcgtag tcgacaaccg
     1921 cgcgcaggcc gtcggcgatc gtctccagca cgtcggcgtg gtcgagcgtc gacagcaggc
     1981 gctcgttgat ctccagcagc cggcgctgtg actggagctg ccgtgccagc tcggccgact
     2041 gcagctccag ccgctcgtag gcggccgcat tggcgaaggc ctgtgccgca tagccggcga
     2101 agatggtcat ggtctgcagg tcgtcgttgg tgaactggtc cttgcccagc ttcgacaggg
     2161 cgatcacgcc caccgagcgc ccctcgaaca gcatggggac caccagcatc gactcctcga
     2221 tgtcatcggt tccgtcgatg gtcagcccgc gcgggtcgtt cagcgcgtcg ttggccagga
     2281 tgggctcgcc atgctcggcg acccagccgg tgaaggaccc ctccccgatc ggcacgcgca
     2341 gcgcctcgcg aaagtcgccc tcgcccagca ggcggtcggt gtaggccacc ggctcgcagt
     2401 caccgcgctc ccagtccacc gcgtacaccc ggatgtcgtg gtaggccgcc agcgtgctgg
     2461 cctcggcaac gatggcatcg gcgatggcct gcacatcggt cagccggttc aggcgagccg
     2521 aaaggtcctg gatggagcgc atgcgtgccg cctggtcggc cacggaccga tagagccgcg
     2581 cgttgctgat ggcgaccgcc gcctggttgg cgaaggcctg cgccaggccc agctcatcgt
     2641 ccggccagtc gcggtcgcgc gagtggtaga ggccgatgag tccgagcgcc cggtcgttgc
     2701 tcaccagcgg caccaggcac gcggtgcgga tggattcggc gcggtaggcg gcctgcatgc
     2761 tgccgatgcc cggcagccga tccgcgtcgc ggacgacgag cgtctttcgc tcgtggacgg
     2821 cccggacgcc gaccgcggtc gagctcaggg tcagccctcg caccgtgtcg tgaaatgcct
     2881 cccccaggcc gcgcgaggcg gcgaccttga aggggtgctc ggtatcgtcc acaagccaga
     2941 ggccgacctt gtccgcctcg aacagggtgc gggtgcgatc gacgatgtcc tccagcaccg
     3001 cgttgaggtc cagcttcccg gtcagctcct gggtcacgtg gcgcagcgcc cgcgccctgg
     3061 ccacctcgcg ccgagcccgg gcgatggcac gggcactgag caggtcggcg ctgacggcgc
     3121 ccgccaccgt gcgcaggaat gaggggcgca cagcctccag tggcccgccg ggctcgtcct
     3181 ccaggacgat cacgccgagg cgttccccgt gggagtgtag cgggacgagg cgcacgggtc
     3241 caccaggcga ggcggcctct ccatccgttt ggtggaagac cgccggcggc ggcagccgat
     3301 gaccggcatg cgtctcgagg gtgagcgttc cagccgaccc ctcgcgggtg aggtagacgg
     3361 cgacccgggc ggcgcccagc tgggtgcgca gggcctcggc cgcggtgcgt gccacggcgg
     3421 ccggggtcga cgaagggccg ccgttgtgtg ccagcagcgc cagcaccgcc agctcggcct
     3481 ggcgctcgcc gggcccatgg gcgcggtcct catcccccgc aacctcgccg ccctcggcca
     3541 gcagccgctc gacgtccccg cgccgatagc ggcggtcacc ccgagcgttg atgcggtagg
     3601 cggtcaggcg accggcatcg gtccaggcgc gaagcgtgtt ggggtggacg cccagcaggg
     3661 aggctgcctg ggcaacgccg agcagcgcat ctggctcggt cgcgcggtcg cgggtaggtt
     3721 gcggcaccct gcgcctcgta gcgtggcctg tgggccggcc ctccgtcgcc gatggaggct
     3781 ttggtaagaa ccggtggacg gatgctagaa ctggcaaggc accacctcga tgccccgttc
     3841 gtaccgagca cggccggtac ccatgccggt tgggtcaggc gaggccggtg aggagcgcca
     3901 ggaccagcgc caccaggccc agcagcatgc ccgcagccac catcccggcg cccgccagca
     3961 gcaggcggcg ccgtggggcg ccaagcaggc gcatggcatc gggcagctcc agcgcggaga
     4021 tgacgtagcc accgggtggt ggcgccaggg tgaggcgtcc gttctcgcgc cgcagctcag
     4081 ccaggaccag gagccgatcc accgtgctga ccacccgggt ggtggagcgc gccggcagcg
     4141 ggttgctccc ggccggaagc aggcggtcga gcgaggcccg gtagtcgggt gcggtcagct
     4201 ccgcggtggt gccgcgccac acatgcggga tggcgaccag cggctccgcc gccgcagccg
     4261 gatccagggg cagggagccg tcgtggtccc acagctcgaa gccgcgcgtc tcgcgcaccc
     4321 gttcgatgct gcgccagccc ctgcccggaa ggcgcacctc cacgtcgcgg tggagcg
//
LOCUS       DATACA010000373         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2056997, whole genome shotgun sequence.
ACCESSION   DATACA010000373 DATACA010000000
VERSION     DATACA010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056997"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VHK28_07740"
     CDS             <1..587
                     /locus_tag="VHK28_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX2142144.1"
                     /translation="AISPDLGDAYYDFVVSLQSEERVESWDPVSESGAGSVGIRFVAL
                     SAAVEIMGRNPLLGVGPGEFEEAFAEVRPDARVPNLQSAHNFLPNLAAEYGLPMLLLV
                     VVAFAVAVLLALPWLDSPDRLARVAAMGVAIAIIAWNAFAAFFGVDLYRTFRLMNADV
                     LQIAVLTALGISLVLARRETRTADESAADGVARV"
     gene            complement(584..1888)
                     /locus_tag="VHK28_07745"
     CDS             complement(584..1888)
                     /locus_tag="VHK28_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX2142145.1"
                     /translation="MAGVFGTRLLTFASTIAVACLLARLLGPEGRGAYSLLLLLPSTL
                     FAVAQFGLPSAVTYFAGGGRSLPSLLGFAAAATIGLSAVLIPLALVALPALQDSVLRA
                     APADLLPLALVGLPIMIASSLFGSILWGRQLVRRYTLILAVQAVNSLLLVLLLVGAAG
                     LDVLGALLAHLLVVGITAVAVAVSAWQASQAEGARDPEGEPRPAGVLELIGYGLRLYP
                     SSLSTFLSYRADLFLLGLLVGDAAQIGLYTLAVSLAEMVFHVPDAVATILYPRVAGSE
                     REEADRIAPSVSRFTVLVTMIGALALIPAAVVAVAVILPDFGGSLLPFFILVPGTVAL
                     SVSKVLSGYISGLGRPLPVSLIAVIGMVVNIGLNFVLIPLMGIAGAALSSAISYSVHA
                     VLTTFVASRLARAPMLAFVIPGRTEVSRLLQSAGQLLRRVRR"
BASE COUNT          345 a          743 c          735 g          242 t
ORIGIN      
        1 gcgccatctc gcccgatctc ggcgacgcct actacgactt cgtcgtctcg ctccagtctg
       61 aggagcgggt cgagtcgtgg gaccccgtct cggagtcggg cgccggatcg gtgggcatcc
      121 gcttcgtggc gctcagcgcc gcggtggaga tcatgggccg caacccgctg ctgggcgtcg
      181 gccccggcga gttcgaggag gcgttcgccg aggtgcgacc cgatgcacgc gtgcccaacc
      241 tgcaatcggc gcacaacttc ctgcccaacc tggccgccga gtacggcctg ccgatgctgc
      301 tgctggtcgt ggtcgccttc gcggtggcgg tgctgctggc gctgccctgg ctggacagcc
      361 ccgaccggct ggcgcgcgtg gcagccatgg gggtggccat cgcgatcatc gcctggaacg
      421 cgttcgccgc gttcttcggg gtggatctgt accggacctt ccgcctcatg aacgccgacg
      481 tgctgcagat cgcggtgctc accgcgctcg gcatctcgct ggtgctcgcg cggcgcgaga
      541 cgcgtacagc tgatgaatcg gcggctgacg gggtggcgcg ggtctagcgg cgcacgcgcc
      601 gcagcagctg cccggcactc tgcagcagcc gtgagacctc ggtccggccc gggatcacga
      661 aggcgagcat cggtgcgcgc gccaggcggc tggcgacgaa cgtggtcagg actgcgtgca
      721 cgctgtacga gatggccgac gacagcgccg ccccggcgat ccccatcagc gggatgagca
      781 cgaagttcag cccgatattg accaccatgc cgatcaccgc gatcaggctc accggcagcg
      841 gccgtcccag cccggagatg tagccggaga gcaccttgga cacgctgagc gccaccgtgc
      901 ccggcaccag gatgaagaac ggcagcaggc tgccgccgaa gtcgggcagg atgacggcca
      961 ccgccaccac ggccgccggg atcagggcca gtgcgccgat catcgtcacc agcaccgtga
     1021 agcgcgagac ggagggagcg atgcgatccg cctcctcgcg ctcgctgccg gcaacgcgcg
     1081 gatagaggat ggtggccacc gcatcgggca cgtggaagac catctcggcc aggctgacgg
     1141 ccagggtgta cagcccgatc tgagccgcat ccccgaccag caggcccagc aggaacaggt
     1201 cggcgcggta gctgaggaag gtgctcagcg acgatgggta gagccgcagg ccgtagccga
     1261 tcagttccag cacaccggcc gggcgcggct caccctccgg gtcccgtgcg ccctcggcct
     1321 gggaggcctg ccaggcggac acggccaccg ccaccgcagt gatgcccacc accagcaggt
     1381 gggccagcag ggcacccagc acgtccaggc cggcggcccc caccagcagc agcaccagca
     1441 gcagggagtt caccgcctgg accgccagga tcagcgtgta gcgacgtacc agctgccggc
     1501 cccacaggat ggagccgaac agggaggagg cgatcatgat cggcaggccc accagcgcca
     1561 ggggcagcag gtcggccggc gccgcgcgca gcacgctgtc ctgcagggcg ggcagcgcca
     1621 ccaacgcgag cgggatgagg accgcggaca gaccgatggt ggccgccgcc gcgaacccca
     1681 gcagcgaggg cagcgagcgg ccgccgcccg cgaagtaggt caccgccgag ggcagcccga
     1741 actgcgccac ggcgaagagc gtgctgggca gcagcaggag caacgagtag gcgccgcggc
     1801 cctcagggcc gagcaggcgc gccagcaggc aggcgaccgc gatggtcgag gcgaaggtca
     1861 gcaggcgcgt gccgaagacc ccggccacgc gacgcgcgaa gggcgacccc ccagccgccg
     1921 gcgccgccgt cacctgccca cgatctcccg gtacatctcc atcacccgct gcgtgaccgc
     1981 ctcagcgcca tgatggcgct cgacccacgc ccgcgaggcc tgaccgagag ccagccgtga
     2041 cggctcgtcg tcgagcagct ccgcc
//
LOCUS       DATACA010000374         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_48051, whole genome shotgun sequence.
ACCESSION   DATACA010000374 DATACA010000000
VERSION     DATACA010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48051"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VHK28_07750"
     CDS             complement(<1..1029)
                     /locus_tag="VHK28_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX2142146.1"
                     /translation="MDERAATDASLDAVLREERSRLTAALVRMLGDWDLAEELVQDAA
                     VTALEHWPSEGVPRNPGAWLMTTARRRAVDRLRRDARYRDRLGLLRQEASDRAPGPPT
                     GGPMDVDDRLRLLFTCCHPALSREAQVALTLRTIGGLDTPAVARAFLVPEATIAQRLV
                     RAKRKIRDAGIPYRVPEGSDLPDRLEQVLAVLYLIFNEGYLSSAGDAPERRELARDAE
                     WLTSLLVRLMPDEPEALGLLALMRLHLARADARFAPDGSLVLLPDQDRSAWDQERIAE
                     AATLVERALRLRRPGPYQLQAAIVAVHASATTYAETDWREIVALYDALYRLQPTPIVA
                     LNRALALAE"
     gene            complement(1051..1509)
                     /locus_tag="VHK28_07755"
     CDS             complement(1051..1509)
                     /locus_tag="VHK28_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2142147.1"
                     /translation="MARLRRSPIGRFVVDNERQQPREARSREVDRPMKYMVLIADSGA
                     WDEMPPEEQQAIYERITSWWGEHAASGRILEGHRLQGTETATTVRRDRDGGVTVMDGP
                     FIEAKETVGGYAIIDVPDLDAAIKLVSGWPGLGPLEIRPLAVGPDAVHDS"
     gene            1530..2528
                     /locus_tag="VHK28_07760"
     CDS             1530..2528
                     /locus_tag="VHK28_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2142148.1"
                     /translation="MTPSRPSAATLPVIVALTIVYIVWGSTYLAIAYVVETMPPFLAA
                     GTRFLAAGAMLVAFLLAQDRWRRGRGAATSRLERPRLVEWRSALIVGVLLLLGGNGMV
                     MVAEQTIPSGIAAVIIATVPIWMSMFDALLTRRAPSRLAVGGLLVGLVGVAILLLPSD
                     GVGALDPVGIGLLVLATISWAAGSLYARHGPLPKNQLLGTGMEQLAGGASLVILALVV
                     GEVGRLEVGSVSRESWIALAYLIVFGSLVAFTAYVWLLNHVAVTTVATYAYVNPIVAV
                     ALGMLFRSEPLTLRTLLAAALIIGAVVAMVSGRPREAEEPGPSPEAASLEPVDKGA"
BASE COUNT          326 a          926 c          897 g          404 t
ORIGIN      
        1 ctcggcgagg gccagcgccc ggttgagcgc cacgatcggc gtgggctgca agcggtacag
       61 cgcatcgtag agcgccacga tctcccgcca gtcggtctcg gcataggtgg tggcggatgc
      121 gtgcacggcg acgatggcgg cctgcagctg gtagggaccc ggccggcgca ggcgaagcgc
      181 ccgctcgacc agcgtggccg cctcggcgat ccgctcctgg tcccaggccg atcggtcctg
      241 gtccggcagg agcaccaggg agccgtccgg ggcgaagcga gcgtccgccc tggccaggtg
      301 gaggcgcatc agcgccagca ggcccagcgc ctccggctcg tccggcatga gccggaccag
      361 gagcgaggtc aaccattccg cgtcgcgcgc cagctctcgg cgctcaggcg catcgccggc
      421 actggagagg tagccctcgt tgaagatcag gtacagcacc gccagcacct gctccaggcg
      481 atccggaagg tcggatccct ccgggacgcg gtaggggatg ccggcgtcgc ggatcttgcg
      541 cttggcgcgc accagccgct gggcgatggt cgcctccggg accaaaaagg cacgcgccac
      601 cgccggcgtg tccaggccgc caatggtgcg cagggtgagc gccacctgcg cctctcggct
      661 cagggcgggg tggcagcagg tgaagagcag ccgcagccga tcgtccacgt ccatcggtcc
      721 accggtgggt ggccccggcg cccggtcaga ggcctcctgg cggagcagcc cgagccggtc
      781 ccggtagcgg gcgtcacgcc gcaggcggtc gacggcgcga cggcgggcgg tggtcatcag
      841 ccaggcgccc gggttgcgcg gcactccctc cgatggccag tgctcgaggg ccgtcaccgc
      901 ggcgtcctgg accagctcct cggcaaggtc ccagtcgccc agcatgcgca ccagcgccgc
      961 ggtcagccgg ctccgctcct cgcggaggac ggcatccagg gatgcatcgg tagccgcgcg
     1021 ctcgtccaac cctcccggtg gtcctccgcc tcagctgtcg tggaccgcgt ccgggccgac
     1081 cgccagcggc cggatctcga gaggtccgag gccgggccat cccgagacga gcttgatggc
     1141 cgcatccagg tccggcacgt cgatgatcgc gtagccaccg acagtctcct tggcctcgat
     1201 gaacgggccg tccatcacgg tcacgccacc gtcccggtca cgccgcacgg tggtggcagt
     1261 ctcggtgccc tgcaggcggt ggccctccag gatcctgccg ctggccgcgt gctctcccca
     1321 ccacgaggtg atgcgctcgt agattgcctg ctgctcctcc ggcggcatct cgtcccacgc
     1381 gccgctgtcg gcgatcagca ccatgtactt catcggtcgg tcgacctctc tgctgcgagc
     1441 ctctcgcggc tgctgccgct cattgtccac gacgaaccgg ccgatggggg atcgacgcag
     1501 ccgggccacg cgtcgggcac aatgcgccgg tgaccccctc gcgtccctcg gctgccacgc
     1561 tgcccgtcat cgtcgcgctg accatcgtct acatcgtgtg gggttccacc tacctcgcca
     1621 tcgcctacgt ggtcgagacc atgccgccct tcctggcggc cggcacccgg ttcctggcgg
     1681 cgggcgccat gctggtcgcc ttcctgctcg cgcaggaccg atggcgccgc gggcgcggcg
     1741 ctgccacctc gcgcctggag cggccacggc tggttgagtg gcgcagcgcg ctgatcgtgg
     1801 gcgtcctgct cctgctgggc gggaacggca tggtgatggt cgccgagcag acgattccct
     1861 ccgggatcgc ggccgtgatc atcgccaccg tcccgatctg gatgagcatg ttcgatgcac
     1921 tgctcacccg gcgtgccccc agccggctgg cggtgggcgg tctgctggtg ggcctggtgg
     1981 gggtcgcgat cctgctcctg ccgtcggacg gcgtgggcgc gctggacccg gtcgggatcg
     2041 gtctcttggt cctggctacc atctcgtggg cggcagggtc cctctatgcc cgtcacggcc
     2101 ccttgcccaa gaaccagctg ctgggcaccg gtatggagca gctagccgga ggtgccagcc
     2161 tggtcatcct ggcactggtc gtcggcgagg tgggccggct ggaggtgggc agcgtctcga
     2221 gagagtcgtg gatcgccctc gcctacctca tcgtgttcgg ctcgctggtc gccttcaccg
     2281 cctacgtctg gctgctgaac cacgtggcgg tgaccacggt ggcgacctat gcctacgtca
     2341 acccgatcgt tgccgtcgca ctcggcatgc tgttccgctc ggagccgctc accctgcgga
     2401 ccctgttggc cgccgcgctg atcatcggcg ccgtggtggc catggtcagc ggccgtcccc
     2461 gcgaggccga ggagcccgga ccctcgccgg aggccgcctc gctcgaaccc gtggacaagg
     2521 gagcctgatc atgcacccca tcgaggtggc ctg
//
LOCUS       DATACA010000375         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_450158, whole genome shotgun sequence.
ACCESSION   DATACA010000375 DATACA010000000
VERSION     DATACA010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450158"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1491
                     /locus_tag="VHK28_07765"
     CDS             <1..1491
                     /locus_tag="VHK28_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEX2142149.1"
                     /translation="EEPTGSPSPQGEEPTPAASSVRPEEAEDQGEPVVRVEILGAEVT
                     PDNLGDPQALSLADTDARAVVDDELELPELDPGAIATVETGFLLGDPERWSPAQPALY
                     VLHVALAGEGVAADDLWTTFGMRHVAVDPQQALIRLNARPVAFTGVGLHDEVIVADPS
                     GAVELLSASRVHSVEPLLEQLERASEVDAELIRAGHTPANPLLLMLADRLGFAVWEEI
                     PLYHYTPLTYGIAMERGIPQQMLREMALRDMNRPSVLFHGLSNESTGTDERADALARL
                     HEIDRQIDGTRLTGQAAYGSLPNDPTQWPLDVAGYTFYYDVFYGDDAALDTARALEEI
                     HALYPDKPVMALEFGRWADGPEGAERQLRIFERTYRELELRSGQRGGYVGGMVWWTLE
                     DFATMVPGIVVEHFGLFNALGEPRPAADAAARLFAAVAGEGGEQTTPTDVGRARVARE
                     PELDLRLFGYLAYALGFSLLLLGGAVALLVHRGGRATAAPLGRSRR"
     gene            1488..1979
                     /locus_tag="VHK28_07770"
     CDS             1488..1979
                     /locus_tag="VHK28_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142150.1"
                     /translation="MRVVHGRLAGVSVWGSFGAGAWVGAALGVVVGAMLGALFAWFCG
                     AILDWQRDLGFTLGITRTLLPFGDQIGALRWISGNWYWVIPGAALAVALISGLVGGVI
                     GALLAAAYNRSPRHATVAVELPEDQAEEVAEAAAAGEEPGIVTPVAPARSRPRSRTGG
                     AGR"
     gene            complement(1918..>2361)
                     /locus_tag="VHK28_07775"
     CDS             complement(1918..>2361)
                     /locus_tag="VHK28_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142151.1"
                     /translation="ATPEVSAQRSPEEGYVEQVRFANSRILQLLDSLLDGPRETWPII
                     VLMADEGPFPQRYAEDEFAFDWPEASEEELFQKFGILTAVLLPDSTPEEAGLYPDITA
                     VNVMRAMLNAEFGTDLELLPDRNWIFTDQRHLYDFVDVTERVPRE"
BASE COUNT          315 a          820 c          878 g          348 t
ORIGIN      
        1 gaggagccta ccggcagccc atcgccgcag ggtgaggagc cgacgccggc ggcgtcatcg
       61 gtccggccgg aagaagcgga ggaccagggg gaaccggtcg tccgggtgga gatcctgggc
      121 gccgaggtca cgcccgacaa cctgggcgat ccgcaggccc tgtcgctggc ggacaccgac
      181 gctcgtgccg tggtcgacga cgagctcgag ctgccggagc tcgacccggg cgcgatcgcg
      241 accgttgaga ccgggttcct gctgggcgat ccggagcgct ggagcccggc gcagccggcg
      301 ctctacgtgc tgcacgtggc gctggccggg gagggcgtgg cggcggacga cctgtggacc
      361 accttcggca tgcgccacgt ggcggtcgac ccgcagcagg cgctcatccg gctcaacgcc
      421 aggccggtgg ccttcaccgg cgtcgggctg cacgacgagg tgatcgtcgc cgacccatcg
      481 ggcgcggtcg agctgctgag cgccagccga gtgcactcgg tcgagccgct gctggagcag
      541 cttgaacgcg ccagcgaggt ggatgccgag ctcatccgtg ccggccacac cccggccaac
      601 ccgctgctgc tgatgctggc cgaccgcctg ggcttcgcgg tctgggagga gatcccgctc
      661 taccactaca cgccgctgac ctatggcatc gccatggagc gcggcatccc ccagcagatg
      721 ctgcgcgaga tggccctgcg cgacatgaac cggccctcgg tcctgttcca cgggctctcg
      781 aacgagtcga ccgggaccga tgagcgggcc gacgccctcg cccggctgca cgagatcgac
      841 cgccagatcg acggcacgcg actgaccggc caggccgcct acggctcgct gccgaacgat
      901 cccacccagt ggccgctgga cgtggccggc tacacgttct actacgacgt gttctacggc
      961 gacgacgccg ccctcgacac ggcccgcgcc ctggaggaga tccacgccct ctacccggac
     1021 aagcccgtca tggccctgga gttcgggcgc tgggcggatg ggccggaggg tgccgagcgc
     1081 cagctgcgca tcttcgagcg cacctaccgc gagctggagc tgcgcagcgg ccagcgcggc
     1141 ggctacgtgg gcggcatggt gtggtggacg ctggaggact tcgccaccat ggtgcccggc
     1201 atcgtggtcg agcatttcgg cctgttcaac gcgctcgggg agccgcgccc ggcggcagac
     1261 gccgcggccc ggctcttcgc ggcggtggcc ggcgagggcg gcgagcagac gacgcctacc
     1321 gacgtcggtc gagcgcgggt cgcacgcgaa ccggagttgg acctgcgcct gttcggctac
     1381 ctcgcctacg ccctgggctt cagcctgctg ctgctgggcg gggcggtcgc gctcctggtc
     1441 caccgcggcg ggcgtgccac cgccgctccc ctggggagga gccgccgatg agggtggtgc
     1501 acggccggct ggcgggggta tcggtctggg gctcgttcgg cgccggcgcc tgggtggggg
     1561 cggcgctggg cgtggtggtc ggcgccatgc tgggcgcgct cttcgcctgg ttctgcggcg
     1621 ccatcctgga ctggcaacgc gacctgggct tcacgctcgg catcacgcgc accctgctgc
     1681 cgttcggcga ccagatcggg gcgctgcgct ggatcagcgg caactggtac tgggtcatcc
     1741 ccggcgccgc gctggcggtg gcgctgatct ccggcctggt gggcggtgtg atcggtgcgc
     1801 tgctggccgc cgcctataac cgctcgccga ggcacgccac ggtggccgtg gagctgccgg
     1861 aggaccaggc cgaggaggtc gccgaggcgg ctgccgccgg cgaggagccg gggatcgtca
     1921 ctcccgtggc acccgctcgg tcacgtccac gaagtcgtac aggtggcgct ggtcggtgaa
     1981 gatccagtta cggtccggca ggagctccag atcggttccg aactcggcgt tcagcatggc
     2041 ccgcatcacg ttcaccgcgg tgatgtccgg gtagagcccg gcctcctcgg gggtgctgtc
     2101 gggaagcagg accgcggtca ggatcccgaa cttctggaag agctcctcct cgctggcctc
     2161 gggccagtcg aaggcgaact cgtcctccgc gtagcgctgc gggaacgggc cttcgtcggc
     2221 catcagcacg atgatcggcc acgtctcacg cgggccgtcc agcaggctgt ccagcagctg
     2281 caagatgcgt gagttggcga agcgcacctg ctccacgtag ccctcctcgg gactgcgctg
     2341 cgcggagacc tccggtgtgg c
//
LOCUS       DATACA010000376         3941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1654760, whole genome shotgun sequence.
ACCESSION   DATACA010000376 DATACA010000000
VERSION     DATACA010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654760"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1101)
                     /locus_tag="VHK28_07780"
     CDS             complement(154..1101)
                     /locus_tag="VHK28_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX2142152.1"
                     /translation="MRFYDAEAMQAAVQPIELLDAVEAAYRDVAAGRDRSPLRTHIAL
                     PSGDLLLMPGMREGAAGTSVKLVTVTPGNAAKGLPTVQAVVAWLDGETGEPTALLDGG
                     TLTRMRTGAASGVATRLLARPDSAVLGLLGAGGQAAWQLRAVLAARPSIELVRVYSRT
                     AASRQAFAAEMAAEVEGRVRVEAAASAEEAVRDADVVCCATTSSTPVFDAGWLATGAH
                     VNGVGAFRLGMVEIPPPAFARAALVTVDSREAALAEAGDLVAALEDGLLAADAVEEIG
                     TVPASWAASREPEAITVFKSVGLAIQDVAAAELILSRLA"
     gene            1173..1508
                     /locus_tag="VHK28_07785"
     CDS             1173..1508
                     /locus_tag="VHK28_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142153.1"
                     /translation="MLTTLRRELGALAWILRTLAMVAVVAAVYRELQRPPEERTWHGR
                     LMGTVPYDFRIPTPRRLAEAWWNPGSDKVITDQPFGVGWSLNLPALLQRITSLGKQSS
                     PHKRSRAAG"
     gene            1566..2021
                     /locus_tag="VHK28_07790"
     CDS             1566..2021
                     /locus_tag="VHK28_07790"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX2142154.1"
                     /translation="MSWFPVLLVIHVSLAVALLAPSVVLPFLLRGSGSAAAARAIPLA
                     RLLLAMQGTGSLVVAVGLAVTGIGLLLVLGMELLSQPWLLVALTIYAANLVVAAFVAR
                     PNLRRLLRLSEADDATWRRRARRQRWIAYAMAAATGVIGLLMSTKPELW"
     gene            2277..3554
                     /gene="cysS"
                     /locus_tag="VHK28_07795"
     CDS             2277..3554
                     /gene="cysS"
                     /locus_tag="VHK28_07795"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX2142155.1"
                     /translation="MTLRLYDTRRRRSVPFAPIGSGPVGLYVCGVTPYDTGHLGHAFT
                     YVSFDVLHRYLEYLGYPVTYVQNLTDVDDDLLRRAHELGEDYLALGNRHVTTFLTEMA
                     ALNWLPPDVYPRATQHVPHMHELIARLLERGHAYVADGHVYFSIDSWERYGELSGLAR
                     EEMLPLANERGNVPDMPGKRDPLDFVLWQPSAADEPSWPSPWGPGRPGWHIECSAMSS
                     AYLGPRFEIHGGGADLIFPHHESEIAQSEGATGESPFVSWWMHAGMLRYDGEKMSKSL
                     GNLVLVRDLLASYPGDAIRHFLVSHHYRDELDFRETDMAASVRSAARLRQACLLAEEI
                     QPFATALADPATLHPPVATNREAFLAAMDDDLRTPLAMPQLEALADLALETDDPALRA
                     EAGWMVRELGARILGLRLATVPGVREAGRAVPA"
     gene            complement(3762..>3941)
                     /locus_tag="VHK28_07800"
     CDS             complement(3762..>3941)
                     /locus_tag="VHK28_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142156.1"
                     /translation="RRHDLAEADADRDQVVVFLRPIAHAANYRRGPLRVLGQSESTGN
                     VGSVRQEREEPDRMG"
BASE COUNT          559 a         1487 c         1334 g          561 t
ORIGIN      
        1 cgatgtacgc ctggtcgcct tcggcactct ctgggaggac gccatgagcc gcctggccga
       61 tgcgctcgtg gcgcagctcg cgggggagtc cccgagcaca gcgctgaact tgcctggccg
      121 ctgacaggcg attcgggcgc ccgccgcgag atctcaggcc agccggctga ggatgagctc
      181 cgcggccgcc acgtcctgga tggcgagccc gacgctcttg aagacggtga tcgcctccgg
      241 ttcccggctg gcagcccagg aggcgggcac cgtgccgatc tcctcgacgg cgtctgcggc
      301 cagcagcccg tcctcgagcg cggccaccag gtcgccggcc tccgccaggg ccgcctccct
      361 cgagtcgacg gtcaccaacg ctgcgcgcgc gaaggccggc ggagggatct ccaccatgcc
      421 aagccggaag gcgccgaccc cgttgacgtg agcgccggtc gccagccagc cggcatcgaa
      481 cacaggcgtg gacgaggtgg tcgcgcagca cacgacgtcc gcatcgcgca ccgcctcctc
      541 ggccgacgcg gcggcctcga cccgcacccg cccctcgacc tccgcggcca tctcggcggc
      601 gaacgcctgt cgtgacgcgg cggtccgcga gtacacgcgc accagctcga tggagggacg
      661 cgccgccagc acggcgcgca gctgccaggc tgcctggcca cctgcaccga gcaagccaag
      721 cacggccgag tcgggacggg cgagcaggcg cgtcgccacc ccggacgcgg cgccggtccg
      781 catccgcgtc agcgtgccgc cgtccaggag tgcggtgggc tcaccggtct cgccatccag
      841 ccaggccacc acggcctgca cggtgggcag ccccttggcc gcattgccgg gagtcaccgt
      901 cacgagcttg acggacgtgc cggcggcacc ctcccgcatg ccgggcatca gcagcaggtc
      961 gccggagggc agcgcgatgt gcgtgcgcag cggcgagcgg tcgcgcccgg cagccacgtc
     1021 gcggtacgcc gcctccacgg cgtccagcag ctcgatcggc tgcaccgccg cctgcatggc
     1081 ctcggcgtca tagaagcgca tagcctgatc ctagtcgccc ccgccggcgg tacgcgccgc
     1141 ctgcggcaca cgacttgcgg gagggcgagg tcatgctgac caccctgcgg cgcgagctcg
     1201 gcgcactggc ctggatccta cggacgctgg cgatggttgc cgtcgtggcc gccgtctaca
     1261 gggagcttca gcgcccgccg gaggagcgca cctggcacgg ccggctgatg ggcaccgtgc
     1321 cgtacgactt ccggatcccg accccgcggc gcctggccga ggcgtggtgg aacccgggca
     1381 gcgacaaggt catcaccgac cagcccttcg gggtcggctg gtccctcaac ctgcccgccc
     1441 tgctgcagcg gatcacctcg ctcggcaagc agtcgagccc tcacaagcgc tcgcgggcag
     1501 ccggctaggc atcgacaccg gaccggcgcg ccgccgaacc atggcggacc gggtagcatc
     1561 ggtccgtgag ctggttcccg gtccttctcg tcatccatgt cagtctggcc gtcgcactgc
     1621 tggctcccag cgtcgtgctg cccttcctgc tccgaggctc ggggagcgcg gccgcggccc
     1681 gcgccatccc gctggcccgg ctgctgctgg ccatgcaggg aaccggctcg ctggtggttg
     1741 cggtcggcct ggcggtgacc ggcatcggtc tgctcctggt gctggggatg gagctcctga
     1801 gccagccgtg gctgctggtg gcgctgacca tctacgccgc caacctggtg gtcgctgcct
     1861 tcgtggcgcg gcccaacctg cggcgcctcc tgcgcctgtc ggaggccgac gacgccacct
     1921 ggcgacggcg tgccaggcgc cagcgctgga tcgcctacgc gatggcggcc gccaccggcg
     1981 tcatcgggct cctgatgagc accaagccgg agctgtggtg aggcgcggaa gtgccggggg
     2041 cagcgggacg cggttcagaa gttcgggatc ggcagcggac gtcccatcag ccagaacagc
     2101 ccggccacga tcaagagcag cgcaatcacg aagacggcta gccagcgcag gacttcccat
     2161 gccatggcgg actcctcggc tcggtcggca gctgacccgt ccccccacgg tcgcggcatc
     2221 atacccgtcg taccgcccaa cgaacaggtc cgcgtgacca ccgcggcgat gtcagcgtga
     2281 ccctccgcct gtacgacaca cgtcggcgcc ggtcggtgcc attcgccccc atcggctccg
     2341 ggccggtggg cctgtacgtg tgcggcgtga cgccgtacga taccgggcac ctggggcacg
     2401 ccttcaccta cgtcagcttc gacgtcctgc atcggtacct cgagtacctg ggctacccgg
     2461 tgacctacgt ccagaacctg accgatgtgg acgacgacct cttgcgccgc gcgcacgagc
     2521 tgggcgagga ctacctggcg ctcggcaacc gccacgtgac caccttcctg accgagatgg
     2581 ccgcgctcaa ctggctgccg cccgacgtct acccccgcgc cacacagcac gtgccgcaca
     2641 tgcacgaact gatcgcgcgc ctgctggagc gcggacacgc ctacgtcgcc gacggccacg
     2701 tctacttcag catcgacagc tgggaacgct acggcgagct gtccggcctg gcccgggagg
     2761 agatgctgcc gctggccaac gagcgcggca acgtgccgga catgcccggc aagcgcgatc
     2821 ccctcgactt cgtgctgtgg caaccatcgg cagccgacga gccgtcatgg ccgtcgccgt
     2881 ggggcccggg gcgccccggc tggcacatcg agtgctcggc aatgagcagc gcctacctcg
     2941 ggccgcggtt cgagatccac ggcggcggcg cggacctgat cttcccccac cacgagtcgg
     3001 agatcgccca gtcggagggc gccaccggcg agtccccgtt cgtcagctgg tggatgcatg
     3061 ccggcatgct gcgctacgac ggcgagaaga tgagcaagtc gctcggcaac ctggtgttgg
     3121 tgcgcgacct cctggcctcc taccccggcg atgccatccg ccacttcctg gtgtcgcacc
     3181 actaccgcga cgagctcgac ttccgcgaga ccgatatggc ggcctccgtg cgctcggccg
     3241 cccgcctgcg ccaggcgtgc ctgctggccg aggaaatcca gcccttcgcc acggcgctcg
     3301 ccgacccggc caccctgcac ccgccggtcg cgacgaaccg cgaggccttt ctggccgcga
     3361 tggacgatga cctgcgcacc ccgctggcca tgccccagct cgaggccctg gccgacctgg
     3421 ccttggagac cgatgacccg gcgctgcgcg ccgaggcggg ctggatggtt cgagaactcg
     3481 gcgcccgcat cctcgggctg cggctggcca cggtccccgg cgtgcgcgag gccggacggg
     3541 ccgtcccggc ctgacccctc ggtcggggcc gcgatggtcc cggtgcgcca cagctgcggc
     3601 agcccgtcag ggatgtgcga catgaccggg gtcatcgtcg cgaatttgtc gtaccggtac
     3661 cgcctaaggg cgacgatccg gacgcccggg ctgccacatc acgccccgtt ggcccgcttc
     3721 gtcggttttg cgccacgccg gtacggcatt taccgacgaa ctcaccccat ccgatcaggt
     3781 tcttcccttt cctgcctcac cgatccgacg ttgcccgttg actcgctctg cccgagcacc
     3841 ctgaggggac cgcgccgata attggccgca tgagcgatcg gccggaggaa gacgacgacc
     3901 tggtcccggt cagcgtccgc ctcggcgagg tcgtgccgcc g
//
LOCUS       DATACA010000377         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_210788, whole genome shotgun sequence.
ACCESSION   DATACA010000377 DATACA010000000
VERSION     DATACA010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210788"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..102
                     /locus_tag="VHK28_07805"
     CDS             1..102
                     /locus_tag="VHK28_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX2142157.1"
                     /translation="MRARGAQVTDIAVIVVAADDGVMPQTRMAVNAS"
     gene            complement(123..698)
                     /locus_tag="VHK28_07810"
                     /pseudo
     CDS             complement(123..698)
                     /locus_tag="VHK28_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            complement(685..927)
                     /locus_tag="VHK28_07815"
     CDS             complement(685..927)
                     /locus_tag="VHK28_07815"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEX2142158.1"
                     /translation="MRTCAACRSVRAKRSMVRLVRQPDGSVVTDPTGKAAGRGTYICQ
                     DPACREPQRLALAVNRALGTAVEPGTLSIEVMNAAT"
     gene            complement(938..2077)
                     /gene="nusA"
                     /locus_tag="VHK28_07820"
     CDS             complement(938..2077)
                     /gene="nusA"
                     /locus_tag="VHK28_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX2142159.1"
                     /translation="MNRDFIGALLQLNAEKGVPQDILIQTLEQAIESAYRRNADAPEN
                     VVVKVDPESGEISVYREYDVVAGDEEIEDPELQMTAAKAQKIDPSTGVGGKVRVPENV
                     TQAQLGRIAAQTAGQVYRQRLREAERDVVYSQYASREGEIMTGVVHRTTDQAVVLDMG
                     KGVEAVLAVTEQLPGEHYRIGQHLKVYVLEVRRTTRGPVLVVSRTHRGFLRRLMEMEI
                     PEIYNGTVVIRGIAREAGSRSKVAVESRQPGVDAKGSAVGQRGARIQAIVAELNGEKV
                     DIVLWNEDAGQFVAEALSPAEVLNVRVDEEHKIANVIVPERQLSLAIGKEGQNARLAA
                     KLTGWRIDIRSDAGVATAAASHDDGDGVEAGATPEEAPADAEATT"
BASE COUNT          282 a          812 c          752 g          385 t
ORIGIN      
        1 atgcgagccc gcggcgcgca ggtcaccgac atcgcggtga tcgtggtggc cgccgacgac
       61 ggcgtcatgc cgcagactcg catggcggtg aacgcctcgt gacccggggt gtccaggaag
      121 acgatgcgcc ggtccttgtg cactatctcg ctggcgccga tgtgctgggt gatgccgccc
      181 cgctcccccg cggccacggt ggtgctgcgg accgcgtcca gcaggctggt cttgccgtgg
      241 tccacgttgc ccatgatggt caccaccggc gctcgggtga ccagctccgc gccctcgtcc
      301 tcggccaccg cctcgcggcg ggcctcggcc gcaaccgtgc cgggcgccgc gatctgctgc
      361 ggctcggcct cgccctcggc cgtggctgcc cgctcggtgc tgccgcgctc aaccgcctcg
      421 attcccagct cgcccgccac cagcgaggcg gtatcgaagt cgatcgtctg attgatggtg
      481 gcaaagatgc cgttgcggat gagctccttg atgatgtcgg cgtgactgac ggtcaggatc
      541 tcggccaggt ccttgacggt gaccgacgac ggcacctcga ccggtccggt cggtcgcgct
      601 ggcagggtcg gggcatccgc caccgccgcg ccacctggcc cgttgcgtcg gcgctgcggg
      661 cgaggcccgc gtcgtggctg gtatctacgt ggcggcattc atcacctcga tgctgagcgt
      721 tcccggctcc acggcggtgc cgagtgctcg gttgactgcc agcgccaggc gttgcggctc
      781 ccggcaggca gggtcttggc agatataggt cccgcggccg gcggccttgc cggtcgggtc
      841 ggtgacgacc gacccatcgg gctggcgtac caggcgaacc atggaccgct tggcgcggac
      901 cgagcggcag gccgcgcagg tccgcatggg aacatgatca ggtcgtcgct tcggcatcgg
      961 ccggcgcctc ctcgggcgtc gcgccagctt cgacgccgtc accatcgtcg tgggaggccg
     1021 ccgccgtggc gaccccggca tcggaccgga tgtcgatccg ccatccggtc agcttggccg
     1081 ccaggcgggc attctgccct tccttgccga tggccaggct gagctgccgc tccggcacga
     1141 tgacgttggc gatcttgtgc tcctcgtcga cgcgcacgtt caggacctcg gccggcgaga
     1201 gcgcctcggc cacgaactgg ccggcgtcct cgttccacag cacgatgtcc accttctcgc
     1261 cgttgagctc ggccacgatg gcctggatcc gggcgccccg ctgccccacg gccgagccct
     1321 tggcatcgac gccgggctgg cggctctcca ccgcgacctt ggagcggctg cccgcctcgc
     1381 gcgcgatgcc gcggatgacg acggtcccgt tgtagatctc cgggatctcc atctccatca
     1441 ggcggcgcag gaagccgcgg tgcgtgcggc tcaccaccag caccgggccg cgcgtggtgc
     1501 ggcgcacctc gagcacgtag accttcaggt gctgaccgat gcggtagtgc tcccccggca
     1561 gctgctcggt gacggccagc accgcctcca cgcccttgcc catgtcgagg acgacggcct
     1621 ggtcggtggt gcggtgcacc acgccggtca tgatctcgcc ctcgcggctg gcgtactggc
     1681 tgtacaccac gtcgcgctcc gcctcgcgca ggcgctgtcg gtagacctgg ccggcggtct
     1741 gcgccgcgat ccggcccagc tgggcctggg tcacgttctc gggcacgcgc accttgccgc
     1801 ccacgccggt cgaggggtcg atcttctggg ccttggcggc cgtcatctgg agctccggat
     1861 cctcgatctc ctcgtcgccg gcgaccacgt cgtactcgcg gtagacgctg atctcgccgg
     1921 actccgggtc gaccttgacc accacgttct cgggggcatc cgcgttgcgt cgataggcgg
     1981 actcgatggc ctgctccagc gtctggatga ggatgtcctg cgggacgccc ttttcggcgt
     2041 tcagctggag cagcgccccg atgaaatctc gattcacgtc cgcacctcgc acccgcggga
     2101 tcaaaacaaa aacgtgggac tggcgaaccg acccacgttt ccctagtcag tatcgattcg
     2161 gctcgcacgg tcgggcgaaa ccgctcagga gtatagcagt ccgccccctc cctggccaac
     2221 gccggctcct c
//
LOCUS       DATACA010000378         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_691669, whole genome shotgun sequence.
ACCESSION   DATACA010000378 DATACA010000000
VERSION     DATACA010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691669"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VHK28_07825"
     CDS             <1..1019
                     /locus_tag="VHK28_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2142160.1"
                     /translation="SGNGPDLGIMHIDQLATNAARQVIVPLDPIAEALKLTEGDFHPT
                     VWNSATYNGQRYGIPLDIHPLGFYYNNAHMQQAGVQQPPTDADSWAQTIQALEQGGVE
                     TPFWVTATWPAHLMFFSLINQFGGSMFNADATQATFASPGGIEALEWYTSWIQNGASP
                     RNVAEDAQATAFKQGRNSLTWDGIWMMNEWKTVSNLDWGVAPVPTIGDQPAVWASSHN
                     FVVMRQPETDQNKLNAARVFISWISENSIEWAKSGQIPARNSVRESQEFQQLDVQSTL
                     AEQLDNVVFPPAVPGIGDITLPTWETAVNEAVLGRKSVRDALTEAQSDANAMLEENRQ
                     KYGA"
     gene            1023..1985
                     /locus_tag="VHK28_07830"
     CDS             1023..1985
                     /locus_tag="VHK28_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012917733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX2142161.1"
                     /translation="MAGTLAPEEAPTRRATKRVNAAAEEESLRTPALTAYLFLLPYLV
                     LFAVFVLAPVVWGIWISLHEWDPLLPLKPFVGLENYTELFDPTSTTSEPFLDAMKATG
                     IFVVLSVPLLVALGLLLAVGLNKRFAGRNMFRALFFAPYVLGVAVVGILWRYLLDPNV
                     GVLNYFLGELGLQADYPWTTQLPWVWAALVIPTLWWTVGYNTVIFLAGLQDIPQELYE
                     AAAIDGAGRWSQFRHVTLPSLRHVTVFVVTITILASANVFGQPYIITQGQPGEETRTA
                     IMEITELGFAQSDMGSASAMSTILTVLLLVVTALSFTLFGRRRA"
     gene            1982..2809
                     /locus_tag="VHK28_07835"
     CDS             1982..2809
                     /locus_tag="VHK28_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX2142162.1"
                     /translation="MRSTSTRAGTYLWLGLLGIIFVAPLAWMVSTSLKSPNEMANPDL
                     NLLPREPTLESYEKILGQSQTPVFQWFVNSILAATLQTVLILITASMAAYALARMQFP
                     GRRLMTGLVLVTLFVPPISLVIPNYLIVDQIGWVDSLAAVIVPGAAGAFGVFFLRQFF
                     LSLPKELEEAAIIDGAGKFRVFWQVIIPLSKPALATLALLSFLTNWNDFLWPLYVLVS
                     PENLTLQPGLSTLQSAYQTDYAAMMAGGVIASVPVLLLFVATQRFVVEGVSRSGLKG"
     gene            2835..>3250
                     /locus_tag="VHK28_07840"
     CDS             2835..>3250
                     /locus_tag="VHK28_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEX2142163.1"
                     /translation="MHPRPQLVRSDWSDLSGQWGFAHDDDDVGLTDGWYESDKPFDRT
                     ITVPFPPESKLSGIHDTGYHPVVWYRSELKVPGPPDGQRLLLHFGAVDYRATVWVDGV
                     RVADHEGGHTPFSCDITHALGNGSKHSIVVRAEDQPT"
BASE COUNT          522 a         1164 c         1046 g          518 t
ORIGIN      
        1 ccagcggcaa cggccccgac ctcggcatca tgcacatcga ccagctcgcc accaacgctg
       61 cgcgccaggt catcgtcccg ctcgacccca tcgccgaggc gctcaagctc accgagggcg
      121 acttccaccc gacggtctgg aactccgcca cctacaacgg acagcgctac ggcatcccgc
      181 tcgacatcca ccccctgggc ttctactaca acaacgcgca catgcagcag gccggcgtgc
      241 agcagccgcc caccgacgcc gactcctggg cgcagacgat ccaggcgctg gagcagggcg
      301 gtgtggagac gccgttctgg gtcaccgcga cctggccggc gcacctgatg ttcttctcgc
      361 tgatcaacca gttcggcggg agcatgttca acgccgacgc cacccaggcg acgttcgcgt
      421 ccccgggggg catcgaggcg ctggagtggt acaccagctg gatccagaac ggcgccagcc
      481 cgcgcaacgt cgccgaggac gcccaggcga cggcgttcaa gcagggccgc aactccctca
      541 cctgggacgg catctggatg atgaacgagt ggaagacggt gagcaacctc gactggggtg
      601 tcgcgccggt tcccacgatc ggtgaccagc cggcggtctg ggccagctcg cacaacttcg
      661 tggtgatgcg ccagcccgag accgaccaga acaagctcaa cgctgcccgc gtgttcatct
      721 cctggatcag cgagaactcg atcgagtggg cgaagtccgg gcagattccc gcgcgcaaca
      781 gcgtccgcga gagccaggag ttccagcagc tcgacgtgca gtcgacgctg gccgagcagc
      841 tcgacaacgt cgtcttcccg ccggcggtcc cgggcatcgg tgacatcacg ctgcccacct
      901 gggagaccgc ggtcaacgag gcggtcctgg gtcgcaagtc ggtgcgcgac gcgctgacgg
      961 aggcccagtc ggacgccaac gcgatgctgg aagagaaccg ccagaagtac ggcgcctgag
     1021 ccatggccgg cacgctcgcg cccgaggagg cgccgacccg gcgcgccacc aaacgggtca
     1081 acgcggcggc ggaggaggag agcctgcgta cgccggcact gacggcgtac ctcttcctgc
     1141 tgccctacct ggtgctcttc gcggtcttcg tgctggcccc ggtggtctgg ggcatctgga
     1201 tcagcctgca cgagtgggat ccgctgctcc cgctcaagcc cttcgtgggg ctggagaact
     1261 acaccgagct gttcgacccg acgtccacca ccagcgagcc gttcctcgac gcgatgaagg
     1321 cgaccgggat cttcgtggtg ctctccgtgc cgctcctggt cgcgctcggc ctgctgctgg
     1381 ccgtcggcct gaacaagcgc ttcgccgggc gcaacatgtt ccgcgcgctc ttcttcgcgc
     1441 cgtacgtgct gggcgtcgcg gtcgtcggca tcctctggcg ctacctgctc gaccccaacg
     1501 tcggcgtgct caactacttc ctgggcgagc tgggcctgca ggccgactac ccgtggacca
     1561 cgcagctgcc gtgggtatgg gcggcgctgg tcatccccac gctgtggtgg accgtcggct
     1621 acaacaccgt catcttcctg gccggcctgc aggacatccc ccaggagctg tacgaggcgg
     1681 ccgccatcga cggcgccggc cgctggagcc agttccggca cgtgacgctt cccagcctgc
     1741 ggcacgtgac ggtgttcgtc gtgacgatca cgatcctggc ctcggcgaac gtgttcgggc
     1801 agccctacat catcacgcag ggacaacctg gtgaggagac gcggacggcg atcatggaga
     1861 tcaccgagct cgggttcgca cagtccgaca tggggtcggc ctcggccatg agcacgatcc
     1921 tcaccgtgct gctgctggtc gtcaccgcgc tgagcttcac cctgttcgga cggaggcggg
     1981 catgaggagc acgagtaccc gcgcgggcac ctacctgtgg ctgggcctgc tgggcatcat
     2041 cttcgtggcg ccgctggcgt ggatggtctc gacatcgctg aagtcgccca acgagatggc
     2101 gaaccccgac ctcaacctgc tgccgcgcga gcccacgctg gagtcctacg agaagatcct
     2161 cgggcagagc cagacaccgg tcttccagtg gttcgtcaac agcatcctgg ccgcgaccct
     2221 gcagacggtg ctcatcctca tcaccgcctc gatggcggcg tacgcgctgg cgcgcatgca
     2281 gttccccggt cgacggctca tgaccggcct ggtgctcgtg accctgttcg tgccgccgat
     2341 ctcgctggtg atccccaact acctcatcgt cgaccagatc ggctgggtgg acagcctggc
     2401 ggcggtcatc gtgcccggcg ccgccggcgc gttcggcgtg ttcttcctgc gccagttctt
     2461 cctctccctg cccaaggagc tggaggaggc cgccatcatc gacggcgcag gcaagttccg
     2521 ggtcttctgg caggtgatca tcccgctgtc gaaaccggcg ctggccaccc tggcgctgct
     2581 gtcgttcctc accaactgga acgacttcct gtggccgctc tacgtgctgg tctcaccgga
     2641 gaacctcacc ctgcagccgg gactgtcgac cctgcagagc gcctaccaga ccgactacgc
     2701 cgcgatgatg gccggcggcg tcatcgccag cgtgcccgtg ctgctcctgt tcgtcgccac
     2761 gcagcggttc gtggtcgagg gcgtctcccg cagcggactg aaaggctgac caccacgtgc
     2821 gaaacaaggc acccgtgcac ccgcggcccc agctggtccg cagcgactgg tccgacctgt
     2881 cggggcagtg gggtttcgcc cacgacgacg acgacgtcgg cctcaccgac ggctggtacg
     2941 agtcggacaa gcccttcgac cgcaccatca ccgtgccgtt cccgccggag tccaagctca
     3001 gcgggatcca cgacaccggc taccacccgg tcgtgtggta ccgctcggag ctgaaggtcc
     3061 ccggaccacc ggacggccag cggctgctgc tgcacttcgg cgcggtggac taccgcgcga
     3121 ccgtgtgggt cgacggagtc cgggtcgccg accacgaggg cgggcacacg ccgttctcct
     3181 gcgacatcac gcacgcgctg ggcaacggtt ccaagcacag catcgtggtc cgcgcggagg
     3241 accagcccac
//
LOCUS       DATACA010000379         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_530060, whole genome shotgun sequence.
ACCESSION   DATACA010000379 DATACA010000000
VERSION     DATACA010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530060"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VHK28_07845"
     CDS             <1..416
                     /locus_tag="VHK28_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2142164.1"
                     /translation="GRTALLFHGTPLKQNEYMWEDRPSRYFGRVAFDEGDDLFCFGHT
                     HQHFHRVVGQAHFVAAGSVGCGSAEDPRARYAVIYVTEADLVVGFRSVEYDYGSVARD
                     LAMAGLSPNPVRVPPVPHPLVDSGMMPLSSAEPAT"
     gene            complement(446..1378)
                     /locus_tag="VHK28_07850"
     CDS             complement(446..1378)
                     /locus_tag="VHK28_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2142165.1"
                     /translation="MTRRIVLDCDPGHDDALAILLAAAHPGLELAAITTVAGNQTLDK
                     VTLNARRVCSVAGIAHVPIAAGCDRPLVREQLVAGSIHGTSGLDGPDWPEPGVGTDDR
                     HAVDLLLELSRGGSPLSVVAVGPLTNVATALDRDPGLAGRLERVVLMGGAIGLGNWTP
                     SAEFNILADPEAAQAVFASGVPITMVPLEVTHQALATDAVLARIDALDTRVGRMAADL
                     LRFFAETYQRVFGFAAPAVHDPCAVACVIDPTIVTTRAMNVEVDTTSELSYGRTVCDV
                     YGTTGRTPNAEVGTALDVERFWELLIGALASYAA"
     gene            complement(1375..2664)
                     /locus_tag="VHK28_07855"
     CDS             complement(1375..2664)
                     /locus_tag="VHK28_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008335183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142166.1"
                     /translation="MGLVTAAATPATGMTRAERAVRTRQRVLGIVYLLVAVAIVIGFA
                     VGIEPGATSTFNLSSRQETIQVPNLVVPSAGTIYLLAGVVAFAGAIQLTRGFGARTAI
                     VLGAVAFAFVFAFLTWVAAGQGMNLTGLLDTTVQRAVPIVFGALSGVLCERAGVVNIA
                     IEGMLLSGAFVSAVLTSVTDSPWLGMLGAIAIGGLLAALLAVLAIRYRVDQIIAGFFI
                     NILVTGLTAFLSVRVLQPYDELVNRPEILGPIRIPLLSEIPILGPVLFQHTIFVYTLF
                     GLVALIHFGLFFTRWGLRVRAVGEHPKAADTVGIDVYRVRYMTVILGGMVAGFGGAYF
                     TIGSTGGFEREMTAGRGFIGLAAMIFGGWSPIGSFLAGLVFGFADAVQSRLSILSIPI
                     PSEFLLMVPYVVTIVVVAGLVGRARAPAADGKPYVKE"
     gene            complement(2753..4039)
                     /locus_tag="VHK28_07860"
     CDS             complement(2753..4039)
                     /locus_tag="VHK28_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142167.1"
                     /translation="MTDDTYRRQPQQEEPEPREPMLEEPDSVSPEVAPEARSDAEPAG
                     GGGRDGGGPPDDAPEAESGSRPGWVRALRGMLLPALALVSAFVIGGLIIVLTDAEAMG
                     LWTSDPLAALGRSWSIVYDSYSALLRGSIGDPIRLFGALVALDWAEVRSAFVPLSETI
                     VSATPLILTGLSVALAFRVGLFNIGAEGQLYIGALVAVIAGFSLVGLPWFIHLPIAVA
                     AGFVGGALWGFVPGLLKAKTGAHEVIVTIMLNFVAFNFINWALKTELVQREGRSDPIS
                     KIIERSAVLPSLVDGLRANWGLVVALAAAVAVWWLLFRSTKGFEFRAVGFNPRAARYA
                     GMSIAWSTVLSMMIAGGLAGLAGAAVILGGSGTLSPGFSPGYGFDGIVVALVGATRPL
                     GVVGAAFLFGALRAGATPMQSATGTPNDIVVVIRRW"
     gene            complement(4032..5606)
                     /locus_tag="VHK28_07865"
     CDS             complement(4032..5606)
                     /locus_tag="VHK28_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142168.1"
                     /translation="MKLELRGITKRFPSVIANQNVDLLVRPGEIHGLLGENGAGKTTL
                     MNVLYGLYQPDEGEILIDDKPVSFQDPGDAIAAGIGMVHQHFMLVPVFTVTENVMLGV
                     ESTRGPVGNLDHQAARRRIVELSEQHNLQVDPDAVVEDLPVGVRQRIEIIKTLYRKSE
                     LLILDEPSAVLTPQEADDLFGVMWSLAKAGTSIIFITHKLNEVMEVADRITVLRRGTV
                     AGTTTPGETSREKLARMMVGRPVQLVVEKKPPQPGEPVLEVSDLVVRDERGVAVVNHL
                     DLTVRAGEIVSIAGVHGNGQSELVDAITGLATPDSGSIRIGGEEVPPDPRAVYRMGVA
                     HIPEDRMEEGLVSGFNIAENLVLNSYWDDPFSSALTMNRDAIARAANEEVENYDIRTP
                     SIFNDVGNLSGGNQQKVIAAREFSRPDVKLIVAAQPTRGLDVGSIEYIHRRIVEQRDA
                     GAAVLLVSTELEEVISLGDRVDVIFQGQIVGRLAPEECTFERLGLLMGGAHPEQSGAE
                     APRATATSAAGATTDD"
BASE COUNT          974 a         1983 c         1984 g          832 t
ORIGIN      
        1 acgggcgcac tgcgctcctc ttccatggca cgccgctcaa gcagaacgag tacatgtggg
       61 aggaccgccc gtcccggtac ttcggccgcg tggccttcga cgagggggac gatctcttct
      121 gcttcggcca cacccaccag cacttccacc gggtggtggg ccaggcgcac ttcgtggcgg
      181 ccggctcggt cggctgcggc agcgccgagg atccgcgggc ccggtacgcg gtcatctacg
      241 tgaccgaggc ggacctcgtt gtgggcttcc gtagcgtgga gtacgactac gggtcggtgg
      301 cgcgcgacct tgccatggcc ggcctgagcc ccaacccggt ccgggtccca ccggtgccgc
      361 acccgctggt cgacagcggg atgatgccgc tcagctcggc ggagccggcg acctagggtt
      421 cggccatggc gcccgcgtga cggcgtcagg cggcatagga cgccagcgcg ccgatcagga
      481 gctcccagaa ccgctcgacg tccagcgccg tgccgacctc ggcgttcggc gtgcggccgg
      541 tggtgccgta gacgtcgcac accgtgcggc cgtaggagag ctccgaggtc gtgtcgacct
      601 ccacgttcat ggcgcgggtg gtcacgatcg tggggtcgat cacgcacgcc acagcacacg
      661 ggtcgtggac cgccggcgcc gcgaagccga agacccgttg gtaggtctcg gcgaagaagc
      721 gcagcaggtc ggcggccatg cggccgaccc gggtgtccag ggcatcgatc cgtgccagca
      781 ccgcgtcggt ggccagcgcc tggtgggtga cctccaaggg caccatggtg atcggcaccc
      841 cggaggcgaa gacggcctgg gccgcctccg gatcggccag gatgttgaac tcggcggacg
      901 gcgtccagtt gcccagaccg atggcgccgc ccatcagcac cacgcgctcc aggcgcccgg
      961 ccaggcccgg atcccggtcg agcgcggtgg ccacgttggt cagcggccca accgccacca
     1021 cgctgagcgg actcccgccg cgcgacagct ccaggagcag gtcgacggcg tgacggtcat
     1081 cggtgcccac gccgggctca ggccagtccg gaccgtccag gccggacgtg ccgtggatgg
     1141 agccggccac cagctgctcg cggaccagcg gccggtcgca gccggccgcg atcggcacgt
     1201 gcgcgatgcc cgccacgctg cacacccgac gagcgttgag ggtcaccttg tccagcgtct
     1261 ggttgccggc cacggtggtg atggccgcca gctccaggcc cgggtgggcg gcggccagca
     1321 ggatggccag cgcatcgtcg tgcccgggat cgcagtccag gacgatccga cgcgtcactc
     1381 cttgacgtag ggcttgccgt cggcggccgg agcgcgcgca cgccccacca gcccggcgac
     1441 gacgacgatg gtgaccacgt agggcaccat cagcaggaac tcggagggaa tcgggatgct
     1501 caggatggag agtcgcgact ggaccgcatc ggcaaatcca aagaccagtc ctgccaggaa
     1561 ggaaccgatg gggctccaac cgccgaagat catggcggcc aggccaatga agccgcgtcc
     1621 cgcagtcatc tcgcgctcga atccgccggt agagccgatg gtgaagtagg cgccgccgaa
     1681 gccggccacc atcccgccca ggatgacggt catgtaacgc acgcggtaga cgtcgatgcc
     1741 caccgtgtca gccgccttgg gatgctcgcc caccgcccgg acgcgaaggc cccagcgggt
     1801 gaagaagagg ccgaagtgga tcagcgccac caggccgaag agggtgtaca cgaagatcgt
     1861 gtgctggaac agcacgggtc ccaggatggg gatttcgctc agcaacggaa tgcggatggg
     1921 ccccaggatc tcgggacggt tgacgagctc gtcgtagggc tgcaggacgc gcaccgacag
     1981 gaaggcggtg agcccggtga ccaggatgtt gatgaagaag ccggcgatga tctggtccac
     2041 gcggtaacga atggccagca cggccaggag ggcggcgagc aggccaccga tggcgatcgc
     2101 gcccagcatg ccgagccacg ggctgtcggt cacgctggtc agcaccgccg atacgaaggc
     2161 gccggacagc agcattccct cgatcgcgat gttcaccacc ccggcgcgct cacacagcac
     2221 gccggaaagc gcaccgaaca cgatgggcac ggcgcgctgc acggtcgtgt ccagcagtcc
     2281 ggtgaggttc atgccctggc cggccgccac ccaggtcagg aaggcgaaga cgaaggcgaa
     2341 ggcgaccgcc cccagcacga ttgccgtgcg tgcgccgaag ccccgtgtca gctggatggc
     2401 gccggcgaag gcgaccaccc cggccagcag gtagatggtc cccgccgacg ggacgaccag
     2461 gttggggacc tggatcgtct cctgccgcga cgacaggttg aaggtcgagg tggcgcccgg
     2521 ttcgatgccc accgcgaagc cgatgacgat cgctaccgcc accagcaggt agacgatgcc
     2581 gaggacgcgt tggcgggtgc gcacggcccg ctcggcgcgg gtcatgccgg tggctggcgt
     2641 ggcggccgcg gtcacgagcc ccatcccttg gcgaagacct cggtaccggt ctgccgctcg
     2701 gcggagatgc ggtagatggc gcgcaccagt gccggagccg cgatgaacat gatcaccagc
     2761 gcctgatgac gaccacgatg tcattcggcg tgccggtggc cgattgcatg ggagtggcgc
     2821 cggcgcgcag cgcgccgaac aggaaggcag cgccaaccac ccccagcgga cgggttgcgc
     2881 cgaccagggc gaccacgatg ccatcgaagc cgtatcccgg cgagaagccc ggcgacagcg
     2941 tccccgaacc gcccaggatc acggcggcac cggccagccc tgccaggccg ccggcgatca
     3001 tcatcgacag cacggtggac caggcgatgc tcatcccggc atagcgagcg gcacgcggat
     3061 tgaagccaac ggcacggaat tcgaagccct tggtggatcg gaagaggagc caccacaccg
     3121 ccaccgccgc cgccagcgcg acgaccaggc cccaattggc ccgcagcccg tccaccaggc
     3181 tgggaagcac cgcgctgcgc tcgatgatct tggagatcgg atccgacctg ccctcacgct
     3241 ggacgagctc cgtcttgagc gcccagttga tgaagttgaa ggcgacgaag ttgagcatga
     3301 tcgtgacgat cacctcgtgg gcgccggtct tcgccttcag taggccggga acgaagcccc
     3361 acagcgcccc gcccacgaat ccggcagcca ccgcgattgg caggtggatg aaccacggca
     3421 gtcccaccag gctgaatccg gcgatgactg ccaccagggc gccgatatag agctgtccct
     3481 ctgcaccgat gttgaagagc ccgacacgaa aggccagcgc caccgatagg cccgtcaaga
     3541 tcagcggcgt ggcggacacg atcgtttccg agagcggcac gaacgcgctc cgcacctcgg
     3601 cccagtccag ggccaccagc gcgccgaaca ggcggatcgg atcgccgatc gagccgcgca
     3661 ggagcgccga gtaggagtcg tagacgatcg accacgaacg acccagcgcc gcgagtgggt
     3721 ccgatgtcca gaggcccatc gcctcggcat cggtcaggac gatgatcagc cctccgatga
     3781 cgaaggcgga caccagggcc agggccggca gcagcatgcc gcgcagtgct cggacccagc
     3841 ccggccgcga cccggactcg gcctccggcg cgtcatccgg cggcccgccg ccgtcccgcc
     3901 cgccaccccc cgctggttcg gcgtcggagc gtgcctccgg cgcaacctcg ggcgacacgc
     3961 tgtccggttc ctccagcatg ggctctcggg gctccggctc ctcctgctgc ggctggcgcc
     4021 ggtaggtgtc gtcagtcatc ggtggtcgcg cctgccgcgg atgtcgccgt cgcccgtggt
     4081 gcctcagcgc ctgactgctc gggatgggca ccgcccatca gcaggcccag gcgctcgaac
     4141 gtgcactcct cgggcgcaag ccgtccgacg atctgaccct ggaagatcac gtcgacgcgg
     4201 tccccgaggc tgatcacctc ctcgagctcg gtggagacca gcagcaccgc cgcgccggca
     4261 tcccgctgct ccacgatccg gcggtggatg tactcgatcg accctacgtc cagcccacgg
     4321 gtgggctgcg ccgcaacgat cagcttgacg tccggccgcg agaattcgcg cgccgcgatc
     4381 accttctgct ggttgccgcc ggagaggttg ccgacgtcgt tgaagatcga cggggtgcgg
     4441 atgtcgtagt tctcgacctc ctcgttcgcg gcgcgggcga tggcgtcacg gttcatggtg
     4501 agggccgacg agaagggatc atcccagtag ctgttgagga cgaggttctc cgcgatgttg
     4561 aacccgctga ccaggccctc ctccatgcga tcctctggaa tgtgggccac ccccatcctg
     4621 tagaccgcac gcggatcggg tgggacctcc tcgccgccga tgcggatgga acccgaatcg
     4681 ggcgtggcga ggccggtgat cgcgtcgacc agctcactct ggccgttgcc gtgcacgccc
     4741 gcgatggaca cgatctcccc ggcgcggacc gtgaggtcca ggtggttgac gaccgccacg
     4801 ccccgctcgt cgcgtaccac caggtcggaa acctccagaa ccggctctcc cggctgtggg
     4861 ggcttcttct ccacaacgag ctgaaccggt cggccgacca tcatgcgcgc cagcttttcg
     4921 cggctggtct ccccgggcgt ggtggttccg gcgaccgtac cgcggcgaag gaccgtgatg
     4981 cggtcggcga cctccatcac ctcgttgagc ttgtgggtga tgaagatgat ggacgtgcct
     5041 gccttggcga gtgaccacat cacgccgaag agatcgtccg cctcctgggg agtgaggaca
     5101 gcggaaggct cgtccaggat caggagctcc gacttgcggt agagggtctt gatgatctcg
     5161 atgcgctgtc gcacgccgac cggcaggtcc tccaccacgg cgtccgggtc gacctgcagg
     5221 ttgtgctgct cggagagctc cacgatccgg cggcgagcgg cctggtggtc caggttgcct
     5281 accggtccgc gcgtgctctc gacacccagc atcacgttct cggtgacggt gaagaccggc
     5341 accagcatga agtgctggtg gaccatcccg atgccggcag cgatcgcgtc ccccgggtcc
     5401 tggaagctga ccggcttgtc atcgatcagg atctcgcctt cgtccggctg gtacagaccg
     5461 tagaggacgt tcatgagcgt ggtcttgccg gcgccattct cgccgagcag cccgtggatc
     5521 tcccccgggc gcaccagcag gtcgacgttc tggttggcga tgaccgaggg aaagcgcttg
     5581 gtgatccctc gcagctcgag cttcacgctg aggtcagctc cggtgtgacg aacgggcggc
     5641 gccgcatgga cgccgcccgt tcaattgtga ctacgccgaa tgagctcggc tcatccgccg
     5701 tagtagtcgg acggggacag gtcaccggcg atgatcgctt cgatcatggc ctcgatctcg
     5761 gccttcacgt cgt
//
LOCUS       DATACA010000380         6787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1334736, whole genome shotgun sequence.
ACCESSION   DATACA010000380 DATACA010000000
VERSION     DATACA010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334736"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1336)
                     /locus_tag="VHK28_07870"
     CDS             complement(158..1336)
                     /locus_tag="VHK28_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142169.1"
                     /translation="MRASVPRWLPWALIGLTLAAAVPAMVLLYINEGSAELMEQVVYA
                     GIFIGYGLVGALVATRRPENAIGWVLLGVSVLTGWGFFGEQYGRYGMITDPGSLPAPE
                     LVGWLGIWLWFPGIGLLLFGLLLFPNGSVPSPRWRPVAWLTAFAVVSISAVFAFTPSS
                     AEDVLPNPFAVLPAGSVEVVEAVFGVTLLLAGLLCLGAPILRFRRAAGVERQQLKWFL
                     YGGALLVVAFVLEPLAAAVPALRPAADLAFPLALAAMPVAIALAILRYGLYEVDRIIS
                     RTIGYALVSATLLAVYAGGALVLSQLTVPFTGDQTVAVAASTLAVVALFRPARRRIQA
                     AVDRRFYRSRYDAQRTLDEFMGSARGEVDVDRLSAQLRSAATSTIHPAAVSVWLRDRV
                     "
     gene            complement(1333..2541)
                     /locus_tag="VHK28_07875"
     CDS             complement(1333..2541)
                     /locus_tag="VHK28_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142170.1"
                     /translation="MGSRTVVLGISALAATTIALSAGGFLLIALNWPSAIEHPEWGFP
                     GFQGAAALVIIGVGIPVVLRRPGNPIGWLMSVAAFLSAAQFVGHQYALHGLVVDPGSV
                     PAPSLGTWVEEWIWIPILAMIAIYPFFLFPTGELISSKWRWPAAVAAPAVAVATLGLA
                     FAPTTRVPGAVNPLAPDGSPQWLELPTVLAVAMVTAVLAVAAGSLVVRFRRASGIERQ
                     QLKWLAYAAVLIAAVLTVYVAGIASGGDVYGPMSTVLAATILFAPVAMAVAILRYGLY
                     EIDILINRTLVYGSLTILLGAVYIGLVLGLQTLLSPVLGTETIAVAMSTLAVAALFGP
                     ARRRVQAAVDRRFYRSRYDAARTLSVFANRLRDEIDFAALTQALLATAERTVQPASAS
                     VWLRERERER"
     gene            complement(2534..3727)
                     /locus_tag="VHK28_07880"
     CDS             complement(2534..3727)
                     /locus_tag="VHK28_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142171.1"
                     /translation="MRPPVARAIAVALFVLGMGMIAYGFASLVLPGTELIPDRPSVFD
                     FVLVALIFIAFPVVGLLVVWQRTTHPIGWLFLVTGITITASVFASEYADRHIHTDVTY
                     PGVEVVAWLGGWTWFLGAGLATTFAVLLFPTGRLPGPRWRPVAWAAGIALATAVVGDA
                     IRPGPVPGYEEVPILKPFAVDGALGEAVAAAAGVAFAAVLALGVVSVSSLAFRFRRSV
                     GQERQQLKWFLYPTALLVVGLAVASVIQTDAAWSAALLGMASIPVCAGIAILRHRLFD
                     IDLVINRTLVYGSLTVLLGGIYIGSVLGLQALITPLLGTDTVAVAASTLAVAALFGPA
                     RHRVQAAVNRRFYRSRYDAQRTLAAFSGRLRDEVDPEHLTDELRAATEATLRPSSAWV
                     WLRDG"
     gene            complement(3753..4082)
                     /locus_tag="VHK28_07885"
     CDS             complement(3753..4082)
                     /locus_tag="VHK28_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2142172.1"
                     /translation="MNVRHPDEGTSIIDIAGELTGESEQELADAQQAASRPGTKLVVL
                     NFTGLEYMNSTGIGLLVTLLIRAQREGQRVVAYGLDEHYREIFSLTRLDEAIAIVDSE
                     ADALAAA"
     gene            complement(4109..4444)
                     /locus_tag="VHK28_07890"
     CDS             complement(4109..4444)
                     /locus_tag="VHK28_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX2142173.1"
                     /translation="MTDRLVTEVRRDGRAGIIDLIGDLDGDAGAALPTAYEQAADGAD
                     RVILNFARMGFMNSSGIALVVELLGRARSAGLSVHAIGLSEHYSRIFEITRLSDFMTI
                     HADEASAVA"
     gene            complement(4441..6018)
                     /locus_tag="VHK28_07895"
     CDS             complement(4441..6018)
                     /locus_tag="VHK28_07895"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX2142174.1"
                     /translation="MSILTSVLGRFGRDQSPPNLAVPTTAELSPAPGAQVEIADHDPF
                     LAYLQAASGPVDLEALELDSPAVRELRAAGVVLVVPLISSGELIGTLNLGPRLSEQGY
                     SSDDRRLLDSLAAQAAPALRVAQLVRQQEAEARERQRIEQELQVAQLIQQHFLPTHLP
                     QPAGWSIEAHYRPARAVGGDFYDVVERPDGRIAFAIGDVTDKGVPAAMVMAATRSLLR
                     SAGTERLTPGQVLAQVNNLLHGDIPSRMFVTCLYGVLDPQTGQVLFANAGHNLPLLRT
                     DDDAVEMRATGMPLGLMPGMAYQEVEGRIGPGQLLLLHSDGITEAHAPDREMFGTGRL
                     MTTVSGEADGRGGVLGRVLAELERFTGIGWEQEDDITLLALRWEGEARIAAPAERSFV
                     SRRGVEREALAWVAEMVDGRGLSKARIENLKTAVGEAVMNAVEHGNRGDESLAVLVEV
                     TPAPGEVIVRITDHGGGRAIPITETPDIDAKLRGEQTTRGWGLFLIQNLVDAVRESSD
                     DTTHTVELVLRTDGGDT"
     gene            6120..>6787
                     /locus_tag="VHK28_07900"
     CDS             6120..>6787
                     /locus_tag="VHK28_07900"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX2142175.1"
                     /translation="MASPKLTVRLLGPPEILLGGTPIRVDTRKATAILAYLIATDQPQ
                     AREHLAALLWPEADDASARGALRRTLSTLRTALDGRWLVVDRASVRLDRGGAWADVWE
                     LRVEGRRQEHADQLLSLLRGEFMAGFGLRDAPEFDDWVLAQADAWRQHAISALDAAAR
                     NLASSGDLPAAIDAAGRRLELDRLDEEGHRQLMELRARAGDRSGAVRQYRSLVRLLAD
                     ELSV"
BASE COUNT         1135 a         2359 c         2392 g          901 t
ORIGIN      
        1 gccgacgact gcgaacgaga gataggtggt ggggtagagg acgatctcgg gcgtgaacgg
       61 ctcgtcgacg ccgaacagcc acagggccat ggcgaggagc atggccagca cggccacgcc
      121 cgaggccgag aacagcgcaa gcctccaatt catcggtcta cacgcggtcg cgcaaccaga
      181 cggagacggc cgccgggtga atggtcgacg tcgcagcgga tcggagctgg gccgaaagac
      241 ggtcgacgtc cacctcgcca cgcgccgagc ccatgaactc gtccagcgtg cgctgcgcgt
      301 cgtatcgcga ccggtagaaa cggcggtcga cggccgcctg gatgcgacgt cgcgccgggc
      361 ggaagagggc gaccacggcc agtgtcgagg ccgcaacggc caccgtctga tccccggtga
      421 aaggcaccgt gagctgggag agcaccagcg cgcccccggc atacactgcc agcagcgtcg
      481 cgctgaccag cgcgtagcca atggtgcggc tgatgattcg atcgacctcg tataggccgt
      541 agcgcaggat ggccagggcg atggccaccg gcatggctgc cagcgccagc gggaaggcca
      601 ggtctgctgc cggccgcaag gcggggacgg cggcggccag aggctcgagg acgaaggcga
      661 ccaccagcag ggcgccgccg tacaggaacc acttgagctg ctgacgctcc acgccggcgg
      721 cgcggcggaa gcgcaggatc ggcgcgccaa ggcatagcag cccggccagc agcagggtca
      781 cgccgaatac ggcctccacg acctccacgc tgcccgccgg caggacggcg aatgggttgg
      841 gcagcacgtc ctccgccgac gacggcgtga aggcgaagac ggcgctgatg ctcaccaccg
      901 cgaacgccgt cagccacgcc accggccgcc agcgtggcga gggcaccgaa ccattgggga
      961 acaggagcag gccgaagagg agcaggccga ttcccgggaa ccacagccag atccccagcc
     1021 agcccaccag ctcgggggcg ggcagcgagc ccgggtcggt gatcatcccg tagcggccgt
     1081 actgctcacc gaagaagccc cagccggtga gcacggagac cccgagcagc acccagccga
     1141 tcgcgttctc cggccggcgc gtcgccacca gcgcgccgac cagcccgtag ccgatgaaga
     1201 tccctgcgta cacgacctgc tccatcagct cggcgctccc ctcgttgata tagagcagca
     1261 ccatggcggg aaccgccgcc gccagcgtca gtccgatcag ggcccagggc agccagcggg
     1321 ggaccgatgc cctcatcgct cccgctcccg ctcccgtagc cagaccgatg cgctggccgg
     1381 ctgcacggtg cgctccgcgg tcgccagcag cgcctgggtc agcgccgcga agtcgatctc
     1441 gtcgcgcaac cggttggcga agacggacag ggtgcgggcc gcgtcgtagc gagaccggta
     1501 gaagcggcgg tcgacggcag cctgcacccg gcgccgcgcg ggtccgaaga gggcggccac
     1561 tgcgagggtc gacatggcca ccgcgatggt ttcggtgccc agcacgggac tgaggagggt
     1621 ctggagcccg agcacgagcc cgatgtacac cgcgcccaga aggatggtga gcgacccgta
     1681 gacaagggtg cggttgatga ggatgtcgat ctcgtacagc ccatagcgca ggatggccac
     1741 cgccatggcc accggggcga acaggatggt ggcggccagt acggtgctca tcggcccata
     1801 gacgtccccg ccgctggcga tgcccgcgac atacacggtc aagacggcgg caatcaggac
     1861 ggcggcgtag gccagccact tgagctgctg acgctcgatg ccggatgctc gtcggaagcg
     1921 gacgaccaat gacccggccg ccacggccag cacggcggtc accatggcga cggccagcac
     1981 ggtcggcagc tccagccatt gcgggctgcc gtccggcgcc agggggttga cggcaccggg
     2041 cacccgcgtc gtcggagcga acgcgagacc cagcgtcgcc accgcgacgg caggagcggc
     2101 gaccgccgcc ggccagcgcc acttcgacga gatcagctcg ccagtgggga acaggaagaa
     2161 ggggtagatc gcgatcatgg ccaggatcgg gatccagatc cactcctcga cccaggtccc
     2221 gagcgacggg gcgggcacgg agccggggtc caccacgagc ccgtgaagcg cgtactggtg
     2281 gccgacgaac tgcgccgccg acaggaaggc agcgaccgac atcagccatc cgatggggtt
     2341 tcccgggcgg cgcagcacga ccgggatgcc aaccccgatg atcaccaacg cagcggctcc
     2401 ctggaagccg gggaaccccc actccgggtg ctcgatcgcg gacggccagt tgagcgcgat
     2461 cagcaggaac ccgccggcgg acagcgcgat cgtcgtggct gccagggcgc tgatgccgag
     2521 cacgacggtc ctgctaccca tcgcgaagcc agacccatgc cgacgacggt cggagtgtcg
     2581 cctcggtggc agcccgcagc tcgtcggtca ggtgctcggg atccacctcg tcgcgcagcc
     2641 ggccggagaa tgccgccagc gtccgttgcg cgtcgtaccg tgaccgatag aagcgccgat
     2701 tgacggcggc ctgcacccgg tgccgggccg gcccgaagag cgccgccacg gccagggtgg
     2761 aggcagcgac cgcgaccgta tcggtcccca gcaaaggcgt gatgagcgcc tgaaggccga
     2821 gcacggagcc gatgtagatg ccgccgagga gcacggtgag cgagccgtag accagggtgc
     2881 ggttgatgac caggtcgatg tcgaacagcc ggtggcgcag gatggcgatg ccggcacaaa
     2941 cggggatgga cgccatgccg agcagtgcgg cactccacgc ggcgtcagtc tgtatcaccg
     3001 aggccacagc cagcccgacc acgaggagcg ccgtcgggta gaggaaccac ttcagctgct
     3061 ggcgctcctg ccctacggag cggcggaagc ggaacgccag cgaggagacc gagaccactc
     3121 cgagggcgag gacggcagcg aatgcgacgc ctgccgcggc cgccacggcc tcaccgagtg
     3181 ccccgtcgac tgcgaagggc ttgaggattg gcacctcttc gtagcccggt acggggccgg
     3241 ggcgaatcgc gtccccgaca acggctgtgg ccagcgcgat gcctgctgcc catgcaaccg
     3301 gccgccatcg cgggccagga agacgtccag tgggaaacag cagaaccgcg aaggtggtgg
     3361 cgagtcctgc accgagaaac cacgtccagc caccgagcca ggcaaccact tccactccgg
     3421 gatacgtgac gtcggtgtgg atgtgccggt ccgcgtactc gctggcgaaa accgacgccg
     3481 tgatggtgat gccggtcacc aggaagagcc agccgatcgg atgcgttgtt cgctgccaga
     3541 cgaccaggag gcccaccacc ggaaaggcga tgaagatgag cgcaaccaac acgaagtcga
     3601 aaacagacgg gcggtccggg atcagctcgg tccccgggag cacgagcgag gcaaacccgt
     3661 aggcaatcat ccccatgccc aggacgaaca gggcgacggc gatcgcccgt gcgacgggcg
     3721 gcctcatgga ccaccccctt ccggagtcga cgtcacgctg ccgcgagggc atcggcctcg
     3781 gagtccacga tggcgatcgc ctcatccagg cgcgtcaggc tgaatatctc gcggtagtgc
     3841 tcgtccaggc cgtaggccac cacccgttgc ccctcccgct gtgcgcggat gaggagcgtc
     3901 accagcagac cgatcccggt gctgttcatg tactccaggc cggtgaagtt gagcaccacc
     3961 agcttcgtgc ccggccgcga tgccgcctgc tgcgcatcgg ccagctcctg ctcgctctcg
     4021 ccggtgagct cgccggcgat gtcgatgatg ctggtcccct catccggatg acgaacgttc
     4081 atggctgcct cctccctcct gcgtcgcgtc aggcgacggc gctggcctca tcggcgtgaa
     4141 tggtcatgaa gtcgctcagc cgcgtgatct cgaagatgcg gctgtagtgc tcgctgagcc
     4201 caatcgcatg gacggatagg cccgcgctcc tggcgcggcc cagcagctcc accacaaggg
     4261 cgatcccgct gctgttcatg aagcccatgc gagcgaagtt gaggatcacg cggtccgctc
     4321 catcggccgc ctgctcgtag gcggtgggca gcgccgctcc ggcatcgccg tccaggtcgc
     4381 cgatcaggtc gatgatgccg gcgcggccgt cgcgccggac ctccgtcacc agtcgatcgg
     4441 tcatgtatca cctccatcgg ttcggagcac gagctccacc gtgtgcgtgg tgtcgtcgct
     4501 cgactcgcgt acggcgtcca ccaggttctg gatcaggaag aggccccagc cgcgcgtcgt
     4561 ctgctcgccg cgcagcttgg cgtcgatgtc cggcgtctcg gtgatcggga tggcccggcc
     4621 gccgccgtgg tcggtgatgc gcacgatgac ctcgccgggc gcgggcgtga cctcgaccag
     4681 cacggccagc gactcgtccc cgcggttgcc gtgctcgacc gcgttcatga ccgcctcccc
     4741 caccgcggtc ttcaggttct cgatccgcgc cttggatagc ccgcgcccgt cgaccatctc
     4801 cgcgacccag gccagcgcct cgcgctcgac gcctcgcctg ctgacgaagc tgcgctccgc
     4861 cggcgcggcg atccgagcct cgccctccca gcgaagggcg agcagggtga tgtcgtcctc
     4921 ctgctcccag ccgatgccgg tgaagcgctc aagctcggcg aggacgcggc ccagcacccc
     4981 gccccgaccg tcggcctcgc ccgacaccgt cgtcatcagg cggccggtgc cgaacatctc
     5041 gcgatcgggc gcgtgcgcct cggtgattcc gtccgagtgg agaagcagca gctggcccgg
     5101 accgatgcgg ccctcgacct cctggtaggc catgccgggc atcaggccca gcggcatgcc
     5161 ggtggcccgc atctcgaccg cgtcgtcatc ggtcctcagc agggggaggt tgtggccggc
     5221 gttggcgaag agcacctgtc cggtctgggg gtcgagcacg ccgtacaggc aggtgacgaa
     5281 catgcgcgac gggatgtcgc cgtgcagcag gttgttgacc tgcgcaagca cctgccccgg
     5341 cgtgagcctc tcagtcccgg ccgagcgcag gagcgaccgc gtggcggcca tgaccatggc
     5401 ggcggggacg cccttgtcgg tgacgtcgcc gatggcgaac gcgatccgcc catccgggcg
     5461 ctcgaccacg tcgtagaagt cgccgcccac cgcccgtgcc ggccgatagt gcgcctcgat
     5521 gctccaaccg gccggttgcg gcaggtgggt gggcaggaag tgctgctgga tgagctgggc
     5581 gacctggagc tcctgctcga tgcgctggcg ctcccgtgcc tcggcctcct gctggcgtac
     5641 cagctgcgcg acgcgaaggg cgggcgccgc ctgtgcggcc agggagtcca gcagccggcg
     5701 gtcgtcgctg gagtagccct gctcggagag ccggggtccc aggttcaacg ttccgatcag
     5761 ctcgccggag ctgatgagcg gcaccaccag caccacgccc gccgcgcgca gctcgcgcac
     5821 cgccgggctg tccagctcga gcgcttcgag gtccaccggg ccgctggccg cctgcaggta
     5881 ggcaaggaat gggtcatggt cggcgatctc gacctgggcg cccggggctg gcgacagctc
     5941 ggccgtcgtc ggtacggcca aattcggtgg cgattggtcg cgaccgaagc ggccgaggac
     6001 agaagtaagg atgctcattg ccgcaatcat gtcccccgtg cgttccgaga acgttacgac
     6061 gcgcgcgctg ccggcgccat acgttacgaa agggactgct gatgccgtac cgtatcggca
     6121 tggcgtcccc caagctcacc gttcgcctgc tgggcccgcc ggagatcctg ctcggtggta
     6181 cgcccatccg cgtcgacacc cgcaaggcga ccgccatcct ggcctaccta atcgccacgg
     6241 accagccgca ggcgcgagag cacctggccg ccctgctctg gccggaggcg gatgacgcct
     6301 cggcgcgggg cgcattgcgc cgcacgctct ccacgctgcg caccgccctg gacgggcgct
     6361 ggctggtcgt ggatcgcgcc tccgtgcgcc tggaccgcgg cggggcgtgg gcggatgtct
     6421 gggagctgcg ggtcgagggc cgccggcagg agcacgccga tcaactgctg agcctgttgc
     6481 gtggcgagtt catggccggc ttcggcctgc gcgacgcgcc ggagttcgac gactgggtcc
     6541 tggcccaggc cgatgcctgg cggcagcacg cgatctccgc cctggacgcc gcggctcgga
     6601 acctggcctc gtccggtgac cttcccgcgg cgatcgacgc ggctggccgg cggctggagc
     6661 tggaccggct cgacgaggag gggcaccggc agctgatgga gctgcgggcg cgtgccggtg
     6721 accggtctgg ggcggtgcgg cagtaccgaa gcctggtccg gctcctcgct gacgagctga
     6781 gcgtgga
//
LOCUS       DATACA010000381         3257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1254682, whole genome shotgun sequence.
ACCESSION   DATACA010000381 DATACA010000000
VERSION     DATACA010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254682"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..656
                     /locus_tag="VHK28_07905"
     CDS             57..656
                     /locus_tag="VHK28_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2142176.1"
                     /translation="MEFQEVVRRRRMVRHFSPEPVERAVLERIAATAQRTPSAGFSQG
                     QRLVIVTDPERMRRVAAIALEEEYVEAGFDPWLSQCAALFVPCVSEKVYRDRYDEPDK
                     RGPDEEAIDWPVPYWWMDIGLTVMLILLAVVDEGLAAGFAGTMEPSRLNEELAIPDHF
                     VPVGVIPVGRPLPDRRSPSLKRGWRPLEEFVRWDSWGEA"
     gene            742..816
                     /locus_tag="VHK28_07910"
     tRNA            742..816
                     /locus_tag="VHK28_07910"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Asn,seq:gtt)
     gene            complement(818..1168)
                     /locus_tag="VHK28_07915"
     CDS             complement(818..1168)
                     /locus_tag="VHK28_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX2142177.1"
                     /translation="MSELLGIARFKFLPGKREEYLRLSAEATELVRANEPGTLGYDLY
                     LNGDQSECMIIERYRDSEAAMEHAANLGHLFEAVLATVEIVHGELLGEPNAELRANLA
                     GSDVPVLFTPYRGE"
     gene            1325..2941
                     /locus_tag="VHK28_07920"
     CDS             1325..2941
                     /locus_tag="VHK28_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142178.1"
                     /translation="MPVHRSCFALPARLTLADAALAVFLLAGVGARVLPGLFTSFPMG
                     DGGLFAVMAADIQAAGWQLPMFASYNGGEIPFTYPPVGLYVLAVLSAILGQTPVQVMD
                     WWPPLLAVASMGAAWLLLSELTDRRTGAWATAVFAITPRSFEWLIVGGGVTRTLGFTM
                     AILAIWLLVLGLREVPHPRGRLPFALGAGAVAGLTLLTHPEATSFGVVSLLALAVRER
                     ARIGQFLRVVLPAGAVAGAIVTPWLVVAIGRHGWQPYADALTSHNSGWVAVEHLLGLD
                     TGAPVPVDPVKIAGAVGLVLLLVHRSWFIPAWYAGLMLLITGAGPTYAMLPLSIAAVL
                     GVRWLAARVTAIWRLPLVAGGVAVAVLFAALAVQQPLSPTRQVSEEAREVMEWVRVNT
                     PPDSRFALVSGTFWWADTEAEWFPALTGRVSVATVQGMEWLDEERWNGSLESHERLQS
                     CAHAGLRCLADWARAYPAEVDYVWRRDLASLPLQDAVARYESGLIAAWERQGFVSASE
                     GCAGGLSDERPPGPRALEACRLASRQLSAL"
     gene            complement(2946..>3257)
                     /locus_tag="VHK28_07925"
     CDS             complement(2946..>3257)
                     /locus_tag="VHK28_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142179.1"
                     /translation="INLGIFLSTFARSEFQAVQFIPLVIVPQILLSGVLVPVESEPEW
                     LQVISNLLPLTYAVDGLRAVMLEGRSLASTALLVDLGVLAGFCVLVIAAAAATLRREI
                     A"
BASE COUNT          436 a         1107 c         1160 g          554 t
ORIGIN      
        1 cccccagtcg catatcggca gtcgttgccc gacgccgcga cggctaggat cagccgatgg
       61 aattccagga ggtcgttcgg cggcgacgca tggtccgcca cttctcgccc gaacccgtgg
      121 agcgcgccgt gctcgaacgc atcgcggcca ccgcgcagcg gacaccgagc gccggcttca
      181 gccagggcca gcggctggtg atcgtgaccg atccggagcg catgcgccgc gtggcggcca
      241 tcgccctgga ggaggagtac gtcgaggcgg gcttcgaccc ctggctcagc cagtgtgcgg
      301 cgctcttcgt gccgtgcgtc agcgagaagg tgtaccgcga ccgatacgac gagccggaca
      361 agcgagggcc cgatgaagag gcgatcgact ggccggtgcc ctactggtgg atggacatcg
      421 ggctgaccgt gatgctcatc ctgttggcgg tggtggacga ggggctggct gcgggcttcg
      481 ccggaaccat ggagccgagc cgcttgaacg aggagctggc catcccggac cacttcgtgc
      541 cggtgggcgt gatcccggtg ggacggccgc tgccggaccg acgctcgcct tcgctcaagc
      601 gtggctggcg gccgctcgag gagttcgtgc gctgggattc gtggggcgag gcctgaccct
      661 caggggagag cggcggacga ggcgtggtat cctctttggc ggcgccgtga tggcgccttt
      721 cgatcgcccc gcggtcgacg ctccggcgta gctcagtggt agagcgggcg gctgttaacc
      781 gcttggtcgt aggttcgaat cctaccgccg gagccactta ctctccgcgg tagggagtga
      841 agagaaccgg cacgtcgctt ccggccaggt tcgccctgag ctccgcgttc ggctcaccga
      901 gcagctcgcc gtggacgatc tcgaccgtag ccaggaccgc ttcgaacaaa tggccgagat
      961 tggcggcgtg ctccatcgct gcctctgagt cccgataccg ctcgatgatc atgcattcgg
     1021 actgatcgcc gttgaggtac aggtcatagc cgagcgtgcc cggctcgttc gcccggacca
     1081 gttcggtggc ctctgcggac aagcgcaggt actcctcgcg ctttcccggc aggaacttga
     1141 atcgcgcgat accaagaagc tcactcatgg gctcagtcct ccaaattcgt gcccggtcga
     1201 cgagggtcga cggggtcggc catggtcgca catcgccctt ccttgccagg cagcggctcg
     1261 aagccgccga gtagcgctcc tcgaggctac ggcctggcac gcaacttgac gtgttgaggc
     1321 gccgatgccc gtgcaccgct cctgcttcgc tctgcccgcc aggctgaccc tggccgatgc
     1381 ggcccttgcc gtgttcctgc tggctggggt cggagcacgc gtgctgcccg gcctcttcac
     1441 ctccttcccg atgggcgatg gcgggctgtt cgcggtgatg gccgccgaca tccaggccgc
     1501 tggctggcag ctgccgatgt tcgcctcgta caacggcggc gagatcccgt tcacctatcc
     1561 gcccgtcggc ctgtacgtgc tggcggtcct ctccgccatc ctgggacaga cgcccgtgca
     1621 ggtcatggac tggtggcccc cgctgctggc cgtggccagc atgggcgccg cctggttgtt
     1681 gctcagcgag ctgacagatc ggcgcaccgg cgcctgggcc accgccgtgt tcgccatcac
     1741 gccccgctcc ttcgagtggc tgatcgtggg cggcggggtg acgcgcacgc tcggcttcac
     1801 catggcgatc ctggccatct ggctccttgt cctcgggctg cgcgaggtcc ctcacccccg
     1861 tggccggctc ccgttcgctc tgggcgccgg cgccgtggcc ggactgacct tgctgaccca
     1921 ccctgaggcc acttcgttcg gcgtcgtctc gctcctggcg ttggcggtgc gagagcgcgc
     1981 ccggatcgga cagttcctcc gcgtggtgct gccggccgga gctgtcgccg gggccattgt
     2041 gacgccgtgg ctggtcgtcg ccatcggccg tcacgggtgg caaccgtacg ctgacgcgct
     2101 gaccagccac aacagcggat gggtggccgt cgagcacctg ctcggtctgg atacgggggc
     2161 cccggtgccc gtcgacccgg tcaagatcgc cggggcggtc gggctggtgc tgctgctggt
     2221 gcatcggtcc tggttcatcc cggcgtggta cgcgggcctg atgctgctca tcaccggcgc
     2281 aggccccacc tacgccatgc tcccgctctc gatcgccgcg gtactgggcg tccgctggct
     2341 ggcagcgcgg gtcaccgcca tatggcgctt gccgctggtc gccggcgggg tggcggtggc
     2401 cgtgctcttc gccgccctgg ccgttcagca gccactgtcg ccgacgcgcc aggtctcgga
     2461 ggaggcgcgc gaggtgatgg agtgggttcg tgtcaacacg ccgcccgaca gccggttcgc
     2521 cctggtgtcc ggcacgttct ggtgggccga tacggaggcc gagtggttcc cggcgcttac
     2581 cggcagggtc agcgtggcca ccgtccaggg catggagtgg ctggacgagg agcgctggaa
     2641 tggaagcctg gagtcgcacg agcgcctgca gtcctgcgct cacgcggggc tgcgctgcct
     2701 ggcggactgg gcgcgcgcgt accccgccga ggtcgactac gtgtggcgcc gggacctggc
     2761 ctcgcttccg ctccaggacg cagtggcccg ttacgagtcg ggcctgatcg cggcctggga
     2821 gcgccagggg ttcgtcagtg cctccgaggg atgtgccggc ggcctcagcg acgagcgtcc
     2881 gcccggcccg cgcgccctgg aggcctgccg tctcgcgtcc cggcagctct ccgcgctcta
     2941 gcagctcagg cgatctcccg gcggagggtg gcggcagcgg cggcgatgac cagcacgcag
     3001 aaaccggcca gcacgcccag gtccacgagg agcgcggtgg aagccaggct tcgcccttcg
     3061 agcatcacgg cgcgcagccc gtcaacggcg taggtcaggg gcagcaggtt ggagatgacc
     3121 tgcagccact cgggctcgct ctccaccgga accaaaacgc cggagagcag gatctgcggc
     3181 acgatcacca gcgggatgaa ctggacggcc tggaactcgc tgcgcgcgaa ggtcgacagg
     3241 aagatgccca ggttgat
//
LOCUS       DATACA010000382         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2379164, whole genome shotgun sequence.
ACCESSION   DATACA010000382 DATACA010000000
VERSION     DATACA010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379164"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VHK28_07930"
     CDS             complement(<1..141)
                     /locus_tag="VHK28_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEX2142180.1"
                     /translation="MKAPRSFRCAHCRSEVPTDAPGTQHRNHCPACLWSRHVDARPGD
                     RRM"
     gene            complement(314..1489)
                     /locus_tag="VHK28_07935"
     CDS             complement(314..1489)
                     /locus_tag="VHK28_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HEX2142181.1"
                     /translation="MNGEVRFSSFLMGGFEGSTHRRSDGRRLDLVASTRHDRFARKDY
                     RRLKDLGVRTVRESLRWHLIEPAPGQCDFGFEAGRVIAAQEEGMQVIWDLAHFGWPDH
                     VDVFSAEFPDAFARYARAAGDFLAMRADGPLLIAPMNEISFLSWAGGEHGLMNPHVVG
                     RGAELKRQLVRASILAIDAVRGRHPDTRFVQPEPLVNIVTDPRRPHERSAAEEARNAQ
                     FETWDMLAGRTAPELGGSSHHLDIVGVNYYPDNQWILGGGSLPADSPLRTELSDMLHE
                     VYARYRRPLVITETGTEDGARPAWLRHVWTEVRRAATRGVPVGGICLYPILNHPGWED
                     DRHCCNGVWDYPGPAGGRQAYGPMRRLILHLQESLSLDGGLLAPYRSVHEISDGGNA"
     gene            complement(1486..2382)
                     /locus_tag="VHK28_07940"
     CDS             complement(1486..2382)
                     /locus_tag="VHK28_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2142182.1"
                     /translation="MKVGLIVPQGWTGEYVGWAPRDAWARSLSIAAEAESLGFDSLWV
                     FDHFHTTPEPTDEITFESFATLAALAGLTNRVELGHVVACAGFRNPALTAKAISTIDV
                     ISGGRVVLGIGAGWKREEWESYGYRFPSTAERLSMLAEQLEIITHMLADGSATFEGDH
                     HRVFGAINQPRGLRSPGIPIMVGGNGPRRTWRIAARFADELNLDAMTPEDVVAATPVI
                     SERCREIGRDPRTLAVSVHVWWEHLEPAGAERRRQLDAYRKAGVRRIQVLLRDAVAHP
                     SALEDFADDCEAAGAALREQVA"
     gene            complement(2379..>2647)
                     /locus_tag="VHK28_07945"
     CDS             complement(2379..>2647)
                     /locus_tag="VHK28_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014850965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEX2142183.1"
                     /translation="TAEGDPYYPIPRPENAALYARYRTLAAAEPSVTFVGRLATYRYY
                     NMDQVVAQALKAAEKLLQAGTGSAAPVPEPARHVTTAEQAAVSA"
BASE COUNT          414 a          914 c          900 g          419 t
ORIGIN      
        1 catgcgccgg tcgccggggc gagcgtcgac gtgccgcgac cacaggcagg ccgggcagtg
       61 gttgcgatgc tgcgtgccgg gagcgtcggt cggaacctcg gaccggcaat gggcgcagcg
      121 gaaggaacgc ggagccttca aacggtgtct cccatcgggc tatcaatggt tggacgagac
      181 cgatggcgtc gcgcgggccc aaccaaagag ccgtccccgg cagggacggc tcgcgcgttc
      241 agcagcgggg ctgagggcta gcggcttcca ctccggaggc gcgacgcggg gaccgtccga
      301 acagtcgttg ccattacgcg ttgcctccgt ccgaaatttc gtgtacggaa cggtacggcg
      361 ccagcaaccc tccgtcaagg gagaggctct cctggagatg gagtatcaat ctccgcatcg
      421 gtccgtacgc ctgccgacct ccggccggcc cggggtagtc ccacacgccg ttgcagcagt
      481 ggcggtcatc ttcccatccg gggtggttga ggatgggata gaggcagatc ccgccaaccg
      541 gcaccccccg ggtcgcggca cgtcgcactt ccgtccatac gtggcgcagc catgcggggc
      601 gagcgccatc ctcggttccg gtctcggtta tgaccagtgg ccgccggtag cgggcgtata
      661 cctcgtgcag catgtcgctc agctctgtgc gcagcgggct gtcagccggc aggctgccgc
      721 cgcccaggat ccactggttg tccgggtagt agttcactcc aacgatgtcc aggtggtgcg
      781 aggacccgcc gagctccggt gccgttctgc ctgcaagcat gtcccatgtt tcgaactgcg
      841 cgttgcgggc ttcttccgct gcgctccgct cgtgaggtcg gcgcggatcc gtaacgatgt
      901 tcaccagggg ctcaggctgc acgaacctgg tgtccgggtg gcggccgcgg accgcgtcga
      961 tggccaggat ggaggcgcgc accagctggc gcttcagctc tgcgccgcgc ccgacaacat
     1021 gagggttcat caggccatgc tctccaccgg cccagctgag gaagctgatc tcgttcatcg
     1081 gcgcgatgag cagcggaccg tccgctcgca tagccaggaa gtccccggcc gcgcgggcat
     1141 atcgcgcgaa tgcgtccggg aactctgcgg agaagacgtc gacgtggtcc ggccaaccga
     1201 agtgggcgag atcccagatg acctgcatcc cctcctcctg agccgcgatc acgcgtccag
     1261 cttcgaagcc gaagtcgcat tggccggggg ccggctcgat cagatgccag cgcagcgact
     1321 cacggacggt tcgaacgccc aggtccttca agcggcggta gtccttccgc gcaaagcggt
     1381 cgtggcgcgt cgacgccact agatccaggc ggcgcccgtc gctgcggcga tgcgtcgagc
     1441 cctcgaagcc gcccatcagg aagctcgaga accggacctc accattcacg ccacctgctc
     1501 ccgcagcgcg gcgccggccg cctcgcaatc gtccgcgaag tcctccagcg ccgagggatg
     1561 cgccaccgcg tcgcggagca gcacctggat cctgcgcacg ccggccttgc gatacgcgtc
     1621 cagctggcgg cgtcgctcgg cgccggccgg ctcgagatgc tcccaccaga cgtggacgga
     1681 aacggccagg gttcgcgggt cgcgcccaat ctcgcggcag cgctcggaga tgaccggggt
     1741 cgcggcgacc acgtcctccg gagtcatggc gtcgaggttc agctcgtccg cgaacctggc
     1801 cgcgatgcgc cacgtccgcc gcgggccatt gccgccgacc atgatcggga tccctggaga
     1861 ccgcagccca cgcggctggt tgatggcacc gaagacacgg tggtggtcgc cctcgaaggt
     1921 ggcactaccg tcggccagca tgtgcgtgat gatctcgagc tgctcggcca gcatcgacag
     1981 gcgttcggcc gtcgacggga accgatagcc ataggactcc cattcctcgc gcttccagcc
     2041 cgccccgatg ccgagcacca cacggccgcc gctgatcacg tcgatggtcg agatggcctt
     2101 ggccgtgagc gccgggttcc ggaatcctgc gcaggcgacc acgtggccca gctccacccg
     2161 attggtgagg ccggcgagcg cggcgagggt ggcgaacgac tcgaaggtga tctcgtccgt
     2221 gggctctggg gtggtgtgaa agtgatcgaa gacccagagc gagtcgaagc ccaggctctc
     2281 cgcctcagcg gcgatcgaca gtgaccgcgc ccacgcgtcc cgtggcgccc atcccacgta
     2341 ttccccggtc cacccctgcg gaacgatcag cccgaccttc acgccgagac cgccgcctgc
     2401 tcagccgtcg tgacatgccg tgccggctcg ggaacgggcg cggctgagcc tgtgcccgcc
     2461 tggaggagct tttcggcggc cttgagggcc tgcgcaacga cctggtccat gttgtagtag
     2521 cggtaggtcg ccagccgccc cacaaaggtt acgctcggct ccgccgcagc cagcgtgcgg
     2581 tagcgtgcgt acagtgcggc gttctccggg cgcggtatcg ggtagtacgg gtcaccctcc
     2641 gccgtcg
//
LOCUS       DATACA010000383         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1174330, whole genome shotgun sequence.
ACCESSION   DATACA010000383 DATACA010000000
VERSION     DATACA010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174330"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VHK28_07950"
     CDS             <1..1615
                     /locus_tag="VHK28_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2142184.1"
                     /translation="RGDRWDVGLLLAAAALLPAGAAAFAVKRPGAERFRLLVPAGAVA
                     AAALTVTSFHPSPYWLIAWLVVALAWPRGWPFVRRIAASVRGDRSRDLAVHVRTPAVE
                     HPEATAHRIRGVRVRDLAALAIALTAFVPVLLIGYEPAIWVVGLALGVVSALALRAGL
                     LGAAWKEAAVPIGCALAIPVFGAGFAEGGGGAVASALAALGALPVADLIAARHPEAGW
                     RRTLFLVSAGMTLAATGAVLLFAVPGQGFASTGHLVIYLLMGFIAVVPGVLVAFSRHP
                     DAAPRLTDRLDTLALSLTPGAAMTVLVHPIGAAMLGAWIVLLVAWRRFTIAPLLGLAA
                     RSQRERDLAVAAVEAERARLAADLHDDALQELSALVRRLDAAGDAEGADLARGVAERL
                     RTITSDLRLPLLDDLGAGPALEWLVGRIRPLADGEVVLERVDPLRPPPGVELAVFRVA
                     QEALANAVKHGSTPIRVRYRVEEDGGVSLSVDDAGPGIEAVAAAEALQAGHLGMANMQ
                     QRAEQIGALLDIRRWPAGGTHVALEWRPR"
     gene            1639..2232
                     /locus_tag="VHK28_07955"
     CDS             1639..2232
                     /locus_tag="VHK28_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX2142185.1"
                     /translation="MGIVDDHPVVRAGTAAIVEEADDLSLVGTAASLEEAAPLLDAAA
                     VDVLLLDLRLGQEFGLTALSHPARPMPAIVVVTSYDYPQYVDAALRLGAAGYVLKTAP
                     ITELLDAIRRAAAGKLSFDVRPGGGGSPLSARERDVVAAVVDGLSNDEIAGRLGISPK
                     TVESHLRRLFERHSVASRTELATRALRDGWLEVPPSD"
BASE COUNT          306 a          910 c          926 g          411 t
ORIGIN      
        1 ccgcggcgat cgctgggacg ttgggttgct gctcgcggca gcggccctct tgccggccgg
       61 cgccgcggcg ttcgccgtga agcgacccgg cgccgagcgg ttccgcctgc tggtaccggc
      121 cggtgccgtc gcggccgcgg cgttgacggt cacctccttc catccatcgc cctactggct
      181 gatcgcgtgg ctggtcgtcg cgcttgcctg gcctcgaggg tggccgttcg tgcgccgcat
      241 cgcggcctcc gttcgcggcg accgatctcg cgatcttgcg gtccacgtgc gaacgccggc
      301 cgtggagcac ccggaagcca ccgcccaccg catccggggc gtgcgcgtcc gcgacctcgc
      361 ggccttagcc attgccctga ccgccttcgt cccggtcctc ctgatcggct acgagccggc
      421 aatctgggtg gttggcctgg cgctgggagt cgtgtctgca ctcgcactgc gcgcaggact
      481 gttgggtgcg gcctggaagg aagcggcggt gcccatcggc tgtgcgctcg ccatcccggt
      541 cttcggcgcg ggcttcgcgg agggcggcgg gggcgctgtt gcgtcagccc tggccgcgct
      601 cggagcactg cccgtggcgg acctgatcgc ggcacgacac cctgaggccg gctggcgtcg
      661 gacgctgttc ctggtgtcgg ccgggatgac gctcgcggcg actggcgccg tgctcctctt
      721 cgccgtccct ggccaggggt ttgcgtccac cggccacctc gtcatctacc tgctgatggg
      781 gttcattgcg gtggtgcccg gggtgctggt tgccttcagc cgccacccgg acgcagcgcc
      841 gcgcctgacc gaccgcctgg acacgctggc cctttcgctg acgcccggcg cggccatgac
      901 cgtgctggtc caccccatcg gtgccgccat gctgggcgcc tggatcgtgc tgctggtcgc
      961 gtggaggcgc ttcacgatcg ctccgctgct ggggctcgcc gcgcgctcgc agcgggaacg
     1021 agacctggcg gtggcggccg tcgaggcaga gcgcgccagg ctggcggccg acctgcacga
     1081 cgacgcgctc caggagctgt cggccctcgt gcgccggctg gatgccgccg gtgacgcgga
     1141 gggagccgac ctggcccgtg gcgtggccga gcggctgcgg accatcacct ccgacctgcg
     1201 cctgccgctg ctggacgatc tcggcgccgg accggccctc gagtggctgg tcggccgcat
     1261 ccggccgctg gcggatggcg aggtcgtgct ggagcgggtc gatccgttgc ggccaccgcc
     1321 cggcgtggag ctcgccgtct tccgcgtggc acaggaggcg ctggccaacg cagtgaagca
     1381 cggcagcaca cccattcgcg tccggtaccg ggtcgaggag gacggcggag tatcgctcag
     1441 cgtcgacgat gcgggccccg gaatcgaggc ggtggcagcc gccgaggccc tgcaggcggg
     1501 gcatctcggc atggcgaaca tgcagcagcg ggccgagcag atcggggcgc tgctcgacat
     1561 ccgacgctgg ccggccggcg ggacccacgt ggcgctcgag tggcggccgc ggtgacggac
     1621 ggtcctgctg ccgtccgggt gggcatcgtc gacgatcatc cggtggtccg cgccggtacg
     1681 gctgcaatcg ttgaggaggc cgatgacctc tcgctggtcg gcaccgccgc ctcgctggag
     1741 gaggccgctc ccctgctcga cgccgcggcc gttgacgtgc tgctgctgga cctgcgcctg
     1801 ggtcaggagt tcggcctcac ggcgctgtcg catccggcga gaccgatgcc ggccatcgtg
     1861 gtcgtgacct cgtacgacta cccgcagtac gtggatgcgg cgttgcgcct gggcgctgcc
     1921 ggctacgtgc tcaagacggc ccccatcacc gagctgctgg acgcgatccg ccgggcagcc
     1981 gccggcaagc tgagcttcga cgtgcgcccc ggcggtggag gctcgccgct cagtgctcgc
     2041 gagcgcgacg tcgtggcggc cgtggtcgac ggcctctcga acgacgagat cgccgggcgg
     2101 ctggggatct cgccaaagac ggtcgagtcg cacctgcggc gcctgttcga gcgacactcg
     2161 gtcgcgtcgc gtaccgagct ggccacgcgc gccctgcgcg acggctggct ggaggtgcct
     2221 ccatcggatt gagcgcactc tcatccgcgc atgtcggtgg cgttaaagaa ctgctcacat
     2281 gtccggggac ccgcaaacgc tcgcaattca caccacttgc tcacgctcgg ttcagaggtg
     2341 cctgggcgac agtcatcggc gggtagctct cttacgcgac caccattcgc acctgagaaa
     2401 gcgaggcggc cccaaggccc tgaggacccg gcctctccat cggtcccgcg agcgcctgcg
     2461 ctagactcac ggctacccat gaccgatgag cagcgggacg accgcatcat cacgcgaacc
     2521 cagctgcagc cgacctcgat ctgggtcggc tgc
//
LOCUS       DATACA010000384         2138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1736610, whole genome shotgun sequence.
ACCESSION   DATACA010000384 DATACA010000000
VERSION     DATACA010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736610"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VHK28_07960"
     CDS             <1..216
                     /locus_tag="VHK28_07960"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2142186.1"
                     /translation="APVGFSLELAGKDLDLITAFADALGVPVPLAAVSRDVIGQAAGA
                     VGGDRDFSAVAVHLRERGRAGEGSTTD"
     gene            275..1708
                     /locus_tag="VHK28_07965"
     CDS             275..1708
                     /locus_tag="VHK28_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2142187.1"
                     /translation="MTDRILIRNATVLTQDESLGEMAGADVLVDGDRIAEVGPGLRAD
                     GARVIEADGDIVIPGFIDTHRHTWETCIRTSAPDFALITYFSAILDKFAPHYRPEDVH
                     SATLWGALECANAGITTLVDWAHIMNTPDHADASVKALQESGIRSVFAYGFPNTSLVD
                     WWFGPDYAGSVLTSDADDARRLKSQYFSSDEGLDGRITMGLATRGPGFCKPEVVRHDW
                     ELAKELGINITVHVAMDRFGYTKGQITALRDMDLLYPGTTYVHASHFTDEEWALVRDS
                     GGNVSFAPQIEIQMGHGWAPAVTAVEYNVPIGLSSDVATTASADQFTQMHAILGSERG
                     RKHQEAWDANLDGLEASPGLITSRDVLRWATLGGAEVAGIADRTGSITPGKKADLVII
                     DTKAVNVSPVIDPVGAVVCAADISNVRTVMVNGELVKDDFRLVASLDGPRRAVEASRD
                     YLISKFGEPEPGWMVGAGAAGEAAAAR"
BASE COUNT          342 a          749 c          718 g          329 t
ORIGIN      
        1 gcgcccgtcg gcttctcgct cgagctggcg ggcaaggacc ttgacctgat caccgccttc
       61 gccgatgccc tcggcgtgcc ggtgccgctg gcggcggtca gccgcgatgt catcggtcag
      121 gctgccggcg ccgtcggcgg cgaccgggac ttctccgcgg tggccgtcca cctccgagag
      181 cgcgggcgcg cgggggaggg cagcaccacc gactgagttt ccccggatct gtaccgagcc
      241 gaatgaaccg ttctgcagaa agggacctgg agccgtgacc gaccgaatcc tcatccgcaa
      301 cgcgaccgtg ctcacccagg acgaatcgtt gggcgagatg gccggcgccg acgtcctcgt
      361 cgacggcgac cgcatcgccg aggtcggccc tggcctcaga gcggacggcg cccgggtgat
      421 cgaggccgat ggggacatcg tcatccccgg cttcatcgac acccaccgcc acacgtggga
      481 gacgtgcatc cggaccagcg ccccggactt cgcgctcatc acctacttca gcgcgatcct
      541 cgacaagttc gcgccgcact accggccgga ggacgttcac tcagcaaccc tgtggggagc
      601 cctcgagtgc gccaacgccg gcatcaccac gctggtcgac tgggcgcaca tcatgaacac
      661 gcccgatcac gcggatgcat cggtcaaggc gctccaggag tctggcattc ggtccgtctt
      721 tgcctacggc ttcccgaaca cctcgctggt cgactggtgg ttcggccccg actatgccgg
      781 cagcgtcctg acctcggacg ccgatgacgc ccgccgcctg aagtcgcagt acttcagctc
      841 cgatgagggg ctcgacggtc gcatcaccat gggcctcgcc acccgcggcc ccgggttctg
      901 caagccggag gtcgtgcgcc acgactggga gctcgccaag gagctcggca tcaacatcac
      961 ggtccacgtc gccatggacc gattcggcta cacgaagggt cagatcaccg cgctacgcga
     1021 catggatttg ctctatcccg gcactaccta cgtgcatgct tcccacttca ccgatgagga
     1081 gtgggcgttg gtgcgcgact ccggcgggaa cgtctccttc gcgccccaga tcgagatcca
     1141 gatgggccac ggctgggcgc ccgcggtcac cgcggtcgag tacaacgtgc cgatcgggct
     1201 ctcgtcggac gtggcgacca ccgcatccgc cgaccagttc acccagatgc acgcgatcct
     1261 gggctccgag cgcggccgca agcaccagga ggcgtgggac gcgaaccttg atggcctcga
     1321 ggcgtcaccc gggctgatca cgtcgcggga cgtgctgcgc tgggcgaccc tcggcggcgc
     1381 ggaggtggcc ggcattgccg accgcaccgg gtccatcacg cccgggaaga aggcggacct
     1441 ggtgatcatc gacacgaagg cggtgaacgt ctcgccggtc atcgacccgg tcggcgcggt
     1501 ggtctgcgcg gccgacatct cgaacgtgcg caccgtcatg gtcaacggcg agctggtgaa
     1561 ggacgacttc cggctggttg cctcgctgga cggaccgcgg cgcgccgtcg aggcctcgcg
     1621 ggactacctc atttccaagt tcggcgagcc agagcccggc tggatggtcg gggccggcgc
     1681 ggccggcgag gcggccgccg cacgctgagt tggttgtggc acgtcgcgtg tcactacagg
     1741 tacaccgatt caccggagcc gcttgttcgg tgctgttctg aacctgtagt tgcacctgcc
     1801 aggcacgagt cgcgcccatc ggtgcagctc ctgcgtgccg ggtgtcactg aagtgacgca
     1861 ttgacaccca actccgcgct ccggcgcata tccgtcacta caggtgcacg ccgaccggcg
     1921 agggtcgata acgagccgct aagcagcgga taagcaccca tcggccatgc tgggccgcat
     1981 ggccacgcga ttggacccgg cgcgaatggc ccgcgagctc gccatgacca tcggccggga
     2041 ggttcagctc gcgaggacta acctggcgct gacgcccacc gcggccgcgc ggctggccaa
     2101 ggtcgcgccg ggcacgcagc ggcgggtgga ggccggcg
//
LOCUS       DATACA010000385         3210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1495986, whole genome shotgun sequence.
ACCESSION   DATACA010000385 DATACA010000000
VERSION     DATACA010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495986"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1874)
                     /locus_tag="VHK28_07970"
     CDS             complement(69..1874)
                     /locus_tag="VHK28_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2142188.1"
                     /translation="MRTTVSTCLALALAWAALGVSPAAAAGTKVTIIVGPTGALTDNY
                     RTAADHIAATAQAAGATVAKVYSPNATWPNVKAAVNGANIIVYLGHGNGSPNPYGSTE
                     LADRHNGWGLNRTTTNGDGDNWSTTMVYCGEKALLGTLTASDGTHQWNWCGGKNNTDG
                     ITPAPGFVMIYNKACYAPGAGEPGHTKATESVALARVRNYSYPALKLGASAYFATDID
                     AGGIVETILENPTMPYWMVAESAPGYSAAAQRQFDHPDIPGAQVWLQRTSGWGGGTDY
                     WLAYAGKPWVSFANPDAPFTSSLEAERHAGADRFATAAAVSAATFGPGVPVAYVATGS
                     DFPDALAGSAAAAVEGGPVLLVGKGAIPSATAGELARLKPAQIVLLGGTGVVGDAVAN
                     ALTSYATTGTVRRLAGNNRFETAAAISADTFLPGVPVAYVATGANFPDALAGTPPAGS
                     QGGPVLLVTKGSVPAATATELARLKPGRIVILGASGVVSNEVAAALGAHTAGSVMRLA
                     GSDRYATAVAVSKASFTTADVVYVATGANFPDALAGGPVAGMGDAPLLLVPGTSVPPS
                     VKQELLRLDPDKVVVLGSTGVVSQGVVYQLRMLFP"
     gene            complement(2024..2878)
                     /gene="mreC"
                     /locus_tag="VHK28_07975"
     CDS             complement(2024..2878)
                     /gene="mreC"
                     /locus_tag="VHK28_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX2142189.1"
                     /translation="MIGSPRRRTRTGLWFAAFAIISMLLLLASRTDAALSLQRVSARA
                     LDPVRQAIGGVGEAVTGVFGVIGEIDRLRTENDDLRRELSAAEQRIAELTEAASENQE
                     LRELLGLTESLEMDLVPVRIISRDPSNFTWEVGIDAGSDQGLSAGMPVVGSAEGAGAL
                     AGTVVSVGADTSTVRLIVDTRSSVVAVDQQSRALGLVRGQLGGQLVMVQVNITEELAE
                     GDSVVTAGLVLEGGGGRSPYPRGLLIGHVQAVEPDPNALTQTGFVRPALDFDRLERLL
                     VVLDFEQS"
     gene            complement(2910..>3210)
                     /locus_tag="VHK28_07980"
     CDS             complement(2910..>3210)
                     /locus_tag="VHK28_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2142190.1"
                     /translation="QIREALEPSVVQIIDAIKETIEETPPELVADIMDQGIVLAGGGA
                     LLAGLDKRVAEATQMPVHIGDDPLTCVVRGTGRVLEDLDALERVLVSEQYTRAPR"
BASE COUNT          455 a         1149 c         1132 g          474 t
ORIGIN      
        1 accggaccgc ggcgaccgcc ggaatggtac ctatggactc tctcccggga ccgcgtccgg
       61 aaacacggct agggaaatag catccgcagc tggtaaacca caccctggct caccactccc
      121 gtgctgccca ggaccacgac cttgtcgggg tccagccgca gcagctcctg cttgacgctc
      181 ggtggcaccg atgtgccggg caccagcagc aatggcgcgt ctcccatgcc ggccaccggg
      241 ccgccggcca gcgcatccgg gaagttcgcc ccggttgcca cgtacaccac gtccgcggtc
      301 gtgaagctgg ccttgctgac ggccaccgcg gtggcgtacc gatcgctgcc cgccagccgc
      361 atcaccgacc cggcggtgtg cgcaccgagc gcggcggcca cctcgttcga caccacgccc
      421 gaggcgccca ggatcacgat cctccccggc ttcaggcgag ccagctcggt tgccgtggcg
      481 gccgggacgg atcccttggt caccagcagc accggtccac cctggctgcc cgccggcggc
      541 gtgccggcca gcgcatcggg gaagttcgcg ccggttgcca cgtaggccac cgggacgccc
      601 ggcaagaagg tatcggccga gatggcggcc gcggtctcga agcgattgtt gccggccagg
      661 cggcgcacgg ttccggtggt ggcgtacgac gtcagtgcgt tggccaccgc gtcaccgacc
      721 acgcccgtcc cgcccagcag cacgatctgc gccggcttca ggcgggccaa ctcgcccgcc
      781 gtggccgatg ggatggcgcc cttgccgacc agcagcacgg gtccgccctc cacggctgca
      841 gcggcactgc ccgccagcgc atcggggaag tcggagccgg tggccacgta ggcgaccgga
      901 acgccggggc cgaaggtggc cgcggagacc gcggcggccg tggcgaaccg atccgcaccg
      961 gcgtggcgtt ccgcctccag cgagctggtg aacggggcat ccgggttggc gaagctcacc
     1021 cacggtttgc cggcgtaggc cagccagtag tccgtgccac caccccagcc gcttgtgcgt
     1081 tggagccaca cctgggcgcc cgggatgtcg gggtggtcga actgacgctg tgcagcggcg
     1141 ctgtagccag gagccgattc ggccaccatc cagtacggca tggtggggtt ctcgaggatc
     1201 gtctcgacga tccctccggc gtcgatgtcg gtggcgaagt acgcgctggc gcccagcttg
     1261 agcgccgggt aactgtagtt tcgcacccgc gccagggcga ccgactcggt ggccttggtg
     1321 tgcccgggct caccggcgcc cggcgcatag caggccttgt tgtagatcat cacgaagccg
     1381 ggggcggggg tgatgccgtc ggtgttgttc ttgccgccgc accagttcca ctggtgcgtg
     1441 ccatctgacg cggtgagcgt gccgagcagc gccttctcac cgcagtagac catcgtcgtc
     1501 gaccagttgt cgccgtcacc gttggtcgtc gtgcggttca ggccccagcc gttgtgccga
     1561 tcggcgagct cggtcgaccc gtagggattg ggcgagccgt tgccgtggcc gaggtagacg
     1621 atgatgttgg cgccgttcac ggcagccttg acgttcggcc aggtggcgtt cggggagtag
     1681 accttggcga cggtggcgcc ggcggcctgg gcggtggccg cgatgtggtc ggcggcggtg
     1741 cggtagttat cggtcagggc gccggtagga ccgacgatga tggtcacctt ggtcccggcg
     1801 gccgcggccg ggctgacgcc cagcgcggcc caggccaggg ccagggcgag gcaggtggag
     1861 acggttgtcc gcaatcggtc gcgcaatgcc ggggttcctc gtcgtcgcta tcggtccagc
     1921 gcaaaccgta ggggcgcacg agcgtgactc ccatgccccg atggtccgaa ggcgcggctg
     1981 accttggggc catgccagcc cggacctgcc gccgcagggt cggtcaggac tgctcgaagt
     2041 cgaggaccac cagcagccgc tccaggcggt cgaagtcgag cgccggtcgc acgaaaccgg
     2101 tctgggtcag ggcgttcggg tcaggctcca ccgcctgcac gtgaccgatg agcaggccgc
     2161 gcgggtacgg gctgcgaccc ccgccgccct ccaggaccag cccggcggtg accaccgagt
     2221 cgccctcggc cagctcctcg gtgatgttga cctgcaccat gaccagctgt ccgccgagct
     2281 ggccgcgcac caggccgagc gcccgtgact gctggtcgac ggccaccacg ctggagcggg
     2341 tgtcgacgat gagccgcacg gtcgacgtgt cggcaccgac cgataccacc gtcccggcca
     2401 gcgcgcctgc gccctccgcc gagccgacga ccggcatgcc cgccgagagt ccctggtcgg
     2461 agccggcatc gatccctacc tcccaggtga agttcgacgg atcgcggctg atgatgcgca
     2521 ccgggaccag gtccatctcc agcgactcag tcagtcccag cagctcgcgc agctcctggt
     2581 tctcgctggc cgcctcggtc agctcggcga tgcgctgctc ggcggccgac agctcgcggc
     2641 gcaggtcgtc gttctcggtg cgcagccggt cgatctcgcc gatcacgcca aagacgccgg
     2701 tgaccgcctc ccccacgccg ccgatcgcct ggcgcacggg gtccagcgcc cgggcgctta
     2761 cgcgctgcag ggagagcgcg gcatcggtcc gcgaggcgag cagcaggagc atggagatga
     2821 tggcgaacgc cgcgaaccac aggccggtgc gggtccggcg gcgcggactg ccgatcatcg
     2881 gtcgggctaa ccgatgcgcc gcgctgcgct cagcgcggcg cgcgcgtgta ctgctcgctg
     2941 accaggacgc gctccagggc gtccaggtcc tcgaggacgc ggccggtgcc gcgcaccacg
     3001 caggtcagcg ggtcgtcgcc gatgtggacc ggcatctggg tcgcttccgc cacccgcttg
     3061 tcgagcccgg ccagcaacgc gccaccgccg gccagcacga tgccctggtc catgatgtcg
     3121 gccaccagct cgggcggcgt ctcctcgatc gtctccttga tggcatcgat gatctgcacc
     3181 accgacggct ccagcgcctc gcgaatctgc
//
LOCUS       DATACA010000386         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_291513, whole genome shotgun sequence.
ACCESSION   DATACA010000386 DATACA010000000
VERSION     DATACA010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291513"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..286
                     /locus_tag="VHK28_07985"
     CDS             35..286
                     /locus_tag="VHK28_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142191.1"
                     /translation="MTQTFVEISRGQSLVGRDGKRIGTVDAVFADYLLVRTSSLLPVD
                     LYVPRNEVSVDEAGEPRVDASAEDAYEAWHSPLRRVAHD"
     gene            279..644
                     /locus_tag="VHK28_07990"
     CDS             279..644
                     /locus_tag="VHK28_07990"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2142192.1"
                     /translation="MTEDDLALLREAEEVRIETSRHTGAPSRRTIIWVVVDDRDRVLV
                     RSVRAERGRWYRDLLANPAGVLHLDGRALGVRAEQVTDGERIEACSTALSRKYADDPA
                     LPSMLRADTLPTTLQLHDA"
     gene            767..1687
                     /gene="paaA"
                     /locus_tag="VHK28_07995"
     CDS             767..1687
                     /gene="paaA"
                     /locus_tag="VHK28_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX2142193.1"
                     /translation="MNEQAQTDAFYAHVMGGGKVEADDWMPDDYRAAVLKFVEMHANS
                     ELMGVLPEREWILRAPTLKRKLALTSKVQDEVGHAQLLYRVAEDLGKPREQMLADLLA
                     GKTKFHNVFHYPTASWGDVGIIAWLVDAAAIVSQQALRDSSYAPYARTMKKICWEESV
                     HIMHGRDVVLSLMNGTDAQRAMVQDALDRWWGPLMQMHGPPTDPAKDRDLIWKVKAKG
                     NEQLRQEFLSIYVPRILELGLTIPDPALRYDEGEGRWHYTEPDWAELKHVVTGHGPMS
                     QQRLEFRRMSRDATAWVRDAIMGSGSVAAA"
     gene            1747..1917
                     /locus_tag="VHK28_08000"
     CDS             1747..1917
                     /locus_tag="VHK28_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142194.1"
                     /translation="MTDEPTERDQNPPPAPDEERRTPPHPSPAETSGQAVERPPRPSQ
                     AEGERELGEQPG"
     gene            1917..2318
                     /locus_tag="VHK28_08005"
     CDS             1917..2318
                     /locus_tag="VHK28_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142195.1"
                     /translation="MAADRRRILVVNDTQEILELFRAILSELGHDMIPMTYAPDDLEQ
                     VLAIKPDLAIIDFVLGGREFLGWQLVQKMRMKPETARIPIIICTAAQREVQEQEGWLA
                     KKGVTVVLKPFDLEELERAVARALRLAEMNG"
     gene            complement(2245..2661)
                     /locus_tag="VHK28_08010"
     CDS             complement(2245..2661)
                     /locus_tag="VHK28_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142196.1"
                     /translation="MEQVADDSGVVRTLRVQGAYYLLSGLTPLVAYRAFVWVTGPKRD
                     DWLVKTFGLVVAGFGAALLRRPSPEVASQAATVAAAIGLAEAWYVLRGRIRTVYLADA
                     VMEGILAARLAAFSRSSRRAAGHERPLAPTPPDRRA"
BASE COUNT          463 a          956 c          908 g          386 t
ORIGIN      
        1 gcgtcgctac cgcccgatcg gctagggtag atgcatgacc cagacgttcg tcgagatcag
       61 ccggggccag tcgctggtcg gtcgggacgg caagcgcatc ggcacggtgg acgccgtctt
      121 cgccgattac ctgctggtcc gtaccagcag cctgctgcca gtggacctct acgtgccgcg
      181 caacgaggtc agcgtcgacg aggcggggga gccacgcgtg gatgcctccg ctgaggatgc
      241 ctacgaggcc tggcacagcc cattgcgccg cgtggcacat gactgaggac gacctggccc
      301 tgctccgcga ggcggaggag gtgcgcatcg agaccagccg ccacaccggg gctcccagcc
      361 gtcgcaccat catctgggtg gtggtcgacg atcgcgaccg ggtcctggtc cgcagcgtgc
      421 gtgctgagcg cggccgctgg taccgcgacc tgctggccaa tccggccggc gtcctgcacc
      481 tggacgggcg tgcccttggg gtccgtgctg agcaggtcac cgatggcgag cggatcgagg
      541 catgctccac cgccctgagc cgcaagtacg ccgacgaccc cgcgctgcct tccatgctgc
      601 gcgccgacac gctgcccacc accctccaac tgcacgacgc gtaggccggg cctgggtttg
      661 tcctgcggat ctcgggctcg cccgaggaac cgctggcgac tcttgggcgg atattgagcc
      721 tcgctcgagc gtggccgtgg catcggtcat gcggtaccct tgaggcgtga acgagcaagc
      781 gcagacggat gccttctatg cccacgtgat gggcggcggc aaggtcgaag ccgacgactg
      841 gatgccggac gactaccgcg ccgccgtgct caagttcgtg gagatgcacg ccaatagcga
      901 gctgatgggc gtgctccccg agcgggagtg gatcctccgc gccccgaccc tcaagcgcaa
      961 gttggccctg acctccaagg tgcaggacga ggtgggccac gcccagctgc tgtaccgcgt
     1021 ggcggaggac ctgggcaagc cccgcgagca gatgttggcc gacctgctgg ccggcaagac
     1081 caagttccac aacgtattcc actaccccac ggcctcgtgg ggcgacgtcg gcatcatcgc
     1141 ctggctggtg gacgcggccg ccatcgtgtc ccagcaggcg ctgcgcgact cgtcctacgc
     1201 gccgtacgcg cgcaccatga agaagatctg ctgggaggag tcggtgcaca tcatgcacgg
     1261 gcgcgacgtg gtcctcagcc tcatgaacgg gaccgatgcc cagcgcgcca tggtgcagga
     1321 cgccctggac cgatggtggg gcccgctgat gcagatgcac ggcccgccca ccgacccggc
     1381 caaggaccgc gacctgatct ggaaggtgaa ggccaagggc aacgaacagc tgcgacagga
     1441 gttcctgtcc atttacgtgc cccgcatcct ggagctcggc ctgaccatcc cggaccccgc
     1501 cctgcgctac gacgagggcg agggccgctg gcactacacc gagccggact gggcagagct
     1561 caagcacgtg gtgaccggcc acggaccgat gtcgcagcag cgcctcgagt tccgtcgcat
     1621 gtcgcgtgac gccaccgcct gggtacgcga cgcgatcatg ggttccgggt cagtcgccgc
     1681 cgcctgatca gctcagcatg ccgcaccctg ggtgccgcgg gcatgcgccc tgctactcca
     1741 cgcggcatga ccgacgaacc gactgagcgc gaccagaacc ctccgcccgc acctgacgag
     1801 gagcggcgca caccgcccca ccccagcccg gccgagacat ccggccaggc cgtggagcgc
     1861 ccgccgcggc caagccaggc cgagggcgaa cgtgagctcg gagagcagcc aggctgatgg
     1921 cagctgaccg tcgccggatc ctggtcgtca acgacacaca ggagatcctg gaactcttcc
     1981 gggccatcct cagtgagctc gggcacgaca tgatccccat gacctacgcc ccggacgacc
     2041 tcgagcaggt gctggccatc aagccggacc tggccatcat cgacttcgtc ctgggcgggc
     2101 gggagttcct cggctggcag ctggtgcaga agatgcgcat gaagccggag acggcccgca
     2161 tcccgatcat catctgcacc gccgcccagc gggaggtcca ggagcaggag ggctggctgg
     2221 ccaagaaggg cgtgaccgtg gtgctcaagc ccttcgatct ggaggagttg gagcgagcgg
     2281 tcgctcgtgc cctgcggctc gccgagatga acggctgaaa gccgcgagcc tcgctgccag
     2341 gatcccctcc attaccgcgt ctgccaggta cacggtgcgg atgcggccgc gcaggacgta
     2401 ccatgcctcc gccagcccga tcgccgcggc gaccgtcgcc gcctgactcg ccacctcggg
     2461 cgagggacgg cggagcaggg ccgcgccgaa gcccgccacc accaggccga acgtcttcac
     2521 cagccagtcg tcgcgcttgg gtccggtgac ccagacgaag gcccgatagg cgacaagcgg
     2581 ggtcaaccca ctcagcaggt agtacgcacc ctgcacgcgc agcgtgcgca ccacgccaga
     2641 atcatcagcc acctgctcca tcggtctcac gccatgcgtt ttgcgcaagg cgcgggccac
     2701 ctattggcac tcg
//
LOCUS       DATACA010000387         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_692996, whole genome shotgun sequence.
ACCESSION   DATACA010000387 DATACA010000000
VERSION     DATACA010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692996"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VHK28_08015"
     CDS             complement(<1..1070)
                     /locus_tag="VHK28_08015"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2142197.1"
                     /translation="MLVPLSWLREYVDVDLPPSELAEQLTLRGMEVQGIAVSGAGWTD
                     VVVGRLLSVDRHPNADKLWLTTVDVGGQEPLQVVCGADNIAPGQLVPVARVGAVLPGE
                     RRIERSKIRGVESQGMLCSAAELELGSDADGIHILAADGGELPLGADLREILGETVLD
                     VDVKPNRGDALSMVGLAREIAAFTGGRLRPPNASVHEDGELRTADLVSVEIADAEGCP
                     RFTARWFEGVANGPSPDWMRRRLVAAGMRPISAVVDVTNYVMHELGQPMHAYDADAVP
                     QGQIVVRRGRPGERLETIDHEERTLDERMLVIADRERAIGLAGIMGGASTEVTDGTTR
                     VILESAVFHGPTIRNTARRLGLR"
     gene            complement(1154..>1565)
                     /locus_tag="VHK28_08020"
     CDS             complement(1154..>1565)
                     /locus_tag="VHK28_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2142198.1"
                     /translation="ALEPPIRVIMPGRCYRYENVAADRNFEFFQIEVLVVDRDTSLAD
                     LKGMLEEFARVLYGPETPTRFRPGYYPFTEPSVAFDIRCQVCGGPGCAACKRSGWVTI
                     LGAGMVHPPVIRNGGYDRPSTRATRSGSAWTGWP"
BASE COUNT          234 a          584 c          523 g          224 t
ORIGIN      
        1 cgcagcccca ggcgcctggc cgtgttgcgg atggtcggcc cgtggaagac cgccgactcc
       61 aggatgaccc gggtcgtgcc gtcggtgacc tcggtggagg cacctcccat gatgccagcc
      121 agtccgatgg cgcgctcccg gtcggcgatg accagcatgc gctcgtcgag cgtccgctcc
      181 tcgtggtcga tggtttccag gcgctccccc ggccgcccgc gccgcaccac gatctgcccc
      241 tgcggcacgg cgtcggcgtc gtaggcgtgc atcggctgcc cgagctcgtg catgacgtag
      301 ttggtgacgt ccaccaccgc cgagatcggc cgcatgccgg cggccaccag ccgacgccgc
      361 atccagtccg gcgagggccc gttggccacg ccctcgaacc agcgcgcggt gaaccgcggg
      421 cagccctcgg cgtcggcgat ctccacgctg accagatcgg cggttcgcag ctccccatcc
      481 tcatggaccg atgcgttcgg cggccgcagc cggccaccgg tgaaggccgc gatctcgcga
      541 gccagcccga ccatggacag cgcatctcct cggttcggct tgacatcgac gtccagcacc
      601 gtctcgccca ggatctcgcg caggtccgcg ccgagcggca gctcaccgcc gtcggcggcc
      661 aggatgtgga tgccgtcggc gtcagagccc agctcgagct cggcggccga gcacagcatc
      721 ccctgcgact cgacgccccg gatcttcgag cgctcgatgc gccgctcccc gggcaggacg
      781 gcacccacgc gggccaccgg caccagctgg cccggcgcga tgttgtccgc tccgcacacc
      841 acctgcagcg gctcctggcc gccgacgtcc acggtggtca gccacagctt gtccgcgttc
      901 gggtgacggt ccacgctgag cagccgccct acgacgacat cggtccatcc ggctcctgag
      961 accgcgatgc cctggacctc catgccccgc agggtgagct gctcggccag ctcggacggc
     1021 ggcagatcga catccacgta ctcgcgcaac caggagagcg gcaccagcat ctgtcagcgc
     1081 cctccgctga actgctcgag gaagcgcagg tccgcgccca gcagctggcg gatgtccgcg
     1141 atgccattcc ggttcatggc cagccggtcc atgccgaacc cgaccgcgta gccctggtac
     1201 tcggacggtc atagccgccg ttgcggatca cggggggatg caccatgccg gcgccgagga
     1261 tggtgaccca gccactccgc ttgcaggcgg cgcagccagg cccgccgcac acctggcagc
     1321 gaatgtcgaa tgccacgctg ggctcggtga acgggtaata gccggggcgg aagcgggtgg
     1381 gcgtctccgg cccgtacagc acgcgcgcga actcctcgag catgcccttg aggtcggcca
     1441 ggctcgtgtc gcggtcgacg accagcacct cgatctggaa gaactcgaag ttgcggtcgg
     1501 cggccacgtt ctcgtagcga tagcagcggc cgggcatgat gacccggatg ggcggctcca
     1561 aggcg
//
LOCUS       DATACA010000388         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2862651, whole genome shotgun sequence.
ACCESSION   DATACA010000388 DATACA010000000
VERSION     DATACA010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2862651"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..175
                     /locus_tag="VHK28_08025"
     CDS             23..175
                     /locus_tag="VHK28_08025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142199.1"
                     /translation="MDRAEAWAREHGCQTMQVASRRTRERAHRFYLREGYTVLKESYV
                     FEKRLG"
     gene            236..2218
                     /gene="dxs"
                     /locus_tag="VHK28_08030"
     CDS             236..2218
                     /gene="dxs"
                     /locus_tag="VHK28_08030"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX2142200.1"
                     /translation="MPILDTITDPAQLRGLSPAELESLAAELREKMIRTVHRTGGHLA
                     SSLGAVELTLALHRVMDSPRDRLVWDTGHQAYAHKLLTGRLDAFDSLRQVGGVGGFPR
                     RGESDHDVFDGGHAGTGVSIGVGLAAARDLRPTADPGRRQRIAVVVGDAAIQSGLTLE
                     ALNHLGHVRHRMLIVLNDNEMSISPSVGGLSTRLNKLRLTRAYQETKSLTQRMLPRVP
                     LVGRPAFEVLGWAKESFKHSWANVAFFEDMGITYIGVLDGHDLDEMEEAFERAFAVDG
                     PVLVHVKTVKGRGYAPAEQDSISFHGASLPPIDLCLIDPTEEPLPPEAEPQRKPKTYT
                     QTFVEEVVRLARADERVVAVTAGMPTGTGLARFCEHFPKRFFDVGIAEQHSVTMATGM
                     ALAGLRPVVALYSTFSQRAYDQLVHDVCQNDAPVLLALDRSGLVGEDGTSHQGMFMLP
                     AMRSLPNLVIGSPKDEQELRDMAVTGLAHPGPMALLYPRDAGEDLPDRVGEALEIGRA
                     ELLREGDDLLLVGFGPIVQRLLVVASALEADGLRAAVVNGRWAKPLDEQCLASMAAGK
                     RLVVTAEESAAMGGFGDGVLDALNRAGVHAPVLKVALAEGFVHHGAVDDLRRQQRIDA
                     DGILEQVRAALGLRRDENGIGAAEPSEGIGTPQRTA"
     gene            2215..3033
                     /locus_tag="VHK28_08035"
     CDS             2215..3033
                     /locus_tag="VHK28_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX2142201.1"
                     /translation="MKPGEHRPPRRQRLDQVLVERGLAPSRPAAQALILAGEVELAGA
                     GTRNLRPGQMVPDDSQVSVRQRPRWASRAGAKLEAALDAFEVDPSGLACLDAGASTGG
                     FTDVLLAHGARRVYAVDVGRAQLVERLRRDPRVVVMERVNLRTLETLPEEVQVATLDL
                     SFISLGLVLPAVRRLLTDDGTVVALVKPQFEAGREAVPRGGVVRDPATHRAVLERFAA
                     DARRADFGPAGLIRSPLAGTDGNLEFLAMLIRQPGMEPAAWDERVRAVTEAAGV"
     gene            3060..3407
                     /locus_tag="VHK28_08040"
     CDS             3060..3407
                     /locus_tag="VHK28_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX2142202.1"
                     /translation="MRKRRAPQVEQLSVGFEGLETIEPAQREPAPDTMRQIVSLRMPD
                     ALMQRVETAARARGVSPSSLMRALIAAGLDRSYAAVQDEGSSDLAGELAELRRQVAAI
                     AGRLGVTEPDSAD"
     gene            3407..>3789
                     /locus_tag="VHK28_08045"
     CDS             3407..>3789
                     /locus_tag="VHK28_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006821589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX2142203.1"
                     /translation="MRIGFTYHPANHLAVDALGRARRWCSAREVDAWDSAADERERVA
                     DACEGTDLVCVLGGDGTFLRTAQAIGDSGVPALGVNLGRVGFLAKVELDDLEGALQQV
                     LAGDYSVEDRFRISATLVRADGSTET"
BASE COUNT          532 a         1312 c         1421 g          524 t
ORIGIN      
        1 cgccggcatc gggcggcggc tcctggacag ggcggaggcc tgggcgcggg agcacggctg
       61 ccagaccatg caggtcgcct cgcggcgcac gcgggagcgg gcgcatcggt tctacctgcg
      121 agaggggtac accgtgctca aggagtcgta tgtcttcgag aagcggctcg ggtgacatcg
      181 gtccccccac aagcgtcagg gctatactcg atcgtcgttt ccccaggagc cgttcgtgcc
      241 gattcttgac accatcaccg accccgcgca gctgcgcggg ctgagcccgg cggagctgga
      301 gagcctcgcc gccgagcttc gcgagaagat gatccggacg gtgcaccgca ccggtggtca
      361 cctggccagc tcgctgggtg ccgtggagct caccctggcc ctgcaccggg tcatggactc
      421 accgcgcgac cggctggtgt gggacaccgg ccaccaggcc tacgcgcaca agctgctgac
      481 cgggcggctg gatgccttcg acagcctgcg gcaggtcggc ggcgtgggcg gcttcccgcg
      541 acgcggtgag agcgatcacg acgtcttcga cgggggacac gccggcaccg gtgtcagcat
      601 cggggtggga ctggcggccg cccgcgacct gcggcccacc gccgatcccg gacgccggca
      661 gcggatcgcg gtcgtggtgg gggatgccgc catccagagc ggcctcaccc tggaggcgct
      721 caaccacctt gggcacgtcc gccaccggat gctgatcgtg ctcaacgaca acgagatgag
      781 catcagcccc tcggtcggcg ggctctcgac gcggctgaac aagctacgcc tgacgcgcgc
      841 ctaccaggag accaagtcgc tcacccagcg catgctgccg cgcgttccgc tggtcgggcg
      901 ccccgccttc gaggtgctcg gctgggcgaa ggagagcttc aagcactcgt gggccaacgt
      961 ggccttcttc gaggacatgg gcatcaccta catcggggtc ctggacggcc atgacctcga
     1021 tgagatggaa gaggccttcg agcgcgcctt cgccgtggac gggccggtgc tggtgcacgt
     1081 caagacggtc aagggccgcg gctacgctcc ggccgagcag gactccatct cgttccacgg
     1141 cgccagcctg ccgcccatcg acctgtgcct gatcgacccc accgaggaac cgcttccgcc
     1201 ggaggcggag ccgcagcgca agccgaagac gtacacccag accttcgtcg aggaggtggt
     1261 gcgcctggca cgggccgatg agcgcgtggt ggcggtgacc gccggcatgc cgaccggcac
     1321 cggcttggcc cgcttctgcg agcacttccc gaagcgcttc ttcgacgttg ggatcgccga
     1381 gcagcactcg gtgaccatgg ccaccggcat ggcgctggcc gggctgcggc cggtggttgc
     1441 cctgtactcg accttcagtc agcgcgccta cgaccagctc gtccacgacg tctgccagaa
     1501 cgacgcgccg gtgctgctgg ccctggaccg atccggcctg gtcggcgagg acggcaccag
     1561 ccaccagggc atgttcatgc tgccggccat gcgttcgctg ccgaacctgg tcatcggctc
     1621 cccgaaggac gagcaggagc tgcgcgacat ggcggtcacc gggttggcgc atcccggacc
     1681 gatggcgctg ctctacccgc gcgacgccgg cgaggatctg ccggaccggg tgggggaggc
     1741 gctggagatc gggcgtgccg agctgctgcg cgaaggcgac gacctcctgc tggtcggctt
     1801 cgggccgatc gtgcagcggc tgctggtggt cgcctcggcg cttgaggccg atgggcttcg
     1861 cgccgcggta gtcaatggcc gctgggcgaa gccgctggac gagcagtgcc tggcctccat
     1921 ggccgccggc aagcggctgg tggtcaccgc cgaggagtcg gccgccatgg gcggattcgg
     1981 cgacggcgtg ctggatgccc tcaaccgcgc cggcgtccac gccccggtgc tgaaggtggc
     2041 gctggccgag ggcttcgtgc accacggcgc ggtggacgac ctgcgccgcc agcagcggat
     2101 cgacgcggac gggatcctgg aacaggtgcg cgccgccctg ggcctcaggc gcgacgagaa
     2161 cgggatcggc gccgcggaac cgagcgaggg gatcggcacg ccgcagcgca ccgcgtgaag
     2221 ccaggcgagc accggccccc gcgcaggcag cggctggacc aggtgctcgt cgagcgcggg
     2281 ctggcacctt cgcgccccgc ggcgcaggcc ctgatcctgg ccggggaggt ggagctcgcc
     2341 ggcgccggca cgcgcaacct gcggcccggg cagatggttc ctgatgacag ccaggtcagc
     2401 gttcgccagc ggccccgctg ggcgagccgt gccggcgcga agctcgaggc ggcgctcgac
     2461 gccttcgagg tcgacccatc cggtctcgcc tgcctggatg ccggcgcatc caccggcggc
     2521 ttcacagacg tgctgctggc ccatggcgca cggcgggtgt atgcggtcga tgtcggccgc
     2581 gcccagctcg tggagcggct gcgccgcgat ccgcgggtgg tggtcatgga gcgcgtgaac
     2641 ctgcggacgc tggagaccct gcccgaagag gtgcaggtgg ccaccctgga cctctcgttc
     2701 atctcgctgg ggttggtgct gcccgccgta cgtcgcctgc tgaccgacga cggaaccgtg
     2761 gtggcgctgg tgaagccgca gttcgaggcc gggcgggagg cggtcccgcg cggcggcgtg
     2821 gtccgcgacc cggcgacgca ccgcgcggtc ctggagcgct tcgccgccga cgcgcggcgc
     2881 gccgacttcg ggccggccgg actgatccga tcgccgctgg ccggcacgga cggcaacctg
     2941 gagttcctcg ccatgctcat ccgacagccc ggaatggagc ccgcggcctg ggatgagcgg
     3001 gtccgggcgg tgaccgaggc ggcgggcgta tgacgcaacg tattacgatg cgcgccggga
     3061 tgaggaaacg cagggcaccg caggtcgagc agctcagcgt cgggttcgag ggcctggaga
     3121 ccatcgagcc cgcccagcgc gagcccgcgc ccgacaccat gcgccagatc gtgtcgctgc
     3181 gcatgccgga cgcgctcatg cagcgggtgg agaccgccgc ccgggcgcgg ggcgtgtcgc
     3241 cgtcgagcct gatgcgcgca ctgatcgcgg cgggtctgga tcggtcctac gccgccgttc
     3301 aggacgaggg gagctccgac ctggccggcg agctcgccga gctgcggcgc caggtggccg
     3361 ccatcgccgg acggctcggc gtgaccgagc ccgactcggc ggactgatgc gcatcggttt
     3421 cacctaccac cccgccaacc atctggcggt cgatgcgctg gggcgcgctc ggcgctggtg
     3481 cagcgcacgc gaggtcgacg cctgggacag cgcagccgac gagcgcgagc gggtggcgga
     3541 cgcctgcgag ggcaccgacc tggtgtgcgt gctgggcggc gacggcacct tcctgcgcac
     3601 cgcgcaggcc atcggcgaca gcggggtgcc ggcgctcggc gtgaacctgg ggcgggtcgg
     3661 cttccttgcc aaggtcgagc tggatgacct ggagggcgcc ctgcagcagg tgctggccgg
     3721 cgactacagc gtagaggatc gcttccggat ctcggccacg ctggtgcggg ccgacggcag
     3781 caccgagac
//
LOCUS       DATACA010000389         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1256312, whole genome shotgun sequence.
ACCESSION   DATACA010000389 DATACA010000000
VERSION     DATACA010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256312"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /gene="truB"
                     /locus_tag="VHK28_08050"
     CDS             complement(<1..389)
                     /gene="truB"
                     /locus_tag="VHK28_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX2142204.1"
                     /translation="MLGVVNLDKPVGPTSHDMVGLLRRLTGTRRIGHAGTLDPLASGV
                     LPILVGAATRFSEELTGGRKRYDALVRLGFRSATDDGEGPLEPVPGPMPSDEAVREAI
                     TGFVGTFEQRPPAFSARKQGGQRAHRAA"
     gene            complement(373..1350)
                     /locus_tag="VHK28_08055"
     CDS             complement(373..1350)
                     /locus_tag="VHK28_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX2142205.1"
                     /translation="MKEATERILAAQRITTVCHENPDADTLGAAIAIAIAAERLGKQA
                     EVVSADPPPPFLAFLPRVETVRRAPQLEPDVAVVVDSGDLSRTGGVAAEHADWLSRAT
                     IVNIDHHVSNTRFGSVDWVDAEAAATCEMIALLLPELGVELDTELATVLMAGIVQDTR
                     TFAHPNATPRTLRVAAQLVEAGAPLSAINRAVYADKPFTTLALWGRMLAAIQQRCEGR
                     IVHASMTLAMLAEIGVEPTASEGFVDLLGSTRAADITILFKEADAETTRVSVRTTERA
                     DAVAITGAFGGGGHARAAGCTVEKPLAEARELVLAECERHLTRSDAGGR"
     gene            complement(1347..1706)
                     /gene="rbfA"
                     /locus_tag="VHK28_08060"
     CDS             complement(1347..1706)
                     /gene="rbfA"
                     /locus_tag="VHK28_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX2142206.1"
                     /translation="MSQRTDRLDSQIQQELMDLLQREMKDPRVGFATITRVETARDLG
                     HARVWVSVYGTDEDRQRSLDALTDATPWLRRRLAERLRIRHVPELTSRRDDSVEAGDR
                     VLRLLQELEQERGTDDR"
     gene            complement(1713..>2286)
                     /locus_tag="VHK28_08065"
     CDS             complement(1713..>2286)
                     /locus_tag="VHK28_08065"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2142207.1"
                     /translation="VQGSLGAIRHALERLNTAEVRISILREGAGDINESDVLLAAASE
                     AVVIGFNTRVDAGAQRVIDSSGVDVRIYDVIYKLTDDLGTAVKGMLTPKLVEQVEGHA
                     EVRMVIKAGRAGNVAGSYVTDGRIVRGGQARLFRGGKQIAESRIESLRRFKDDVREVA
                     TGFECGIGLAIDDIAEGDVIECYQMVAEQA"
BASE COUNT          318 a          834 c          794 g          340 t
ORIGIN      
        1 gcggcacggt gggcgcgctg cccgccctgc ttccgcgccg agaaggccgg cggccgctgc
       61 tcgaaggtac ccacgaaccc ggtgatcgcc tcgcgaaccg cctcgtcgga cggcatcggc
      121 cccggaactg gctccagcgg gccctcgccg tcatcggtcg ccgagcggaa gcccaggcgc
      181 acaagggcgt cataccgctt gcgtccgccg gtcagctcct cgctgaagcg ggtggcggcg
      241 ccgaccagga tgggaaggac cccggaggcc agtggatcga gcgtgccggc atggccgatt
      301 cgccgggtgc cggtgaggcg tcgcagcagg ccgaccatgt cgtgcgaggt cggccccacg
      361 ggcttgtcga ggttaacgac ccccagcatc gctccgcgtg aggtgacgct cgcactcggc
      421 gagcaccagc tctcgcgcct cggcgagcgg cttctccacg gtgcagccgg ccgcgcgagc
      481 gtggcccccg ccgccgaagg cgccggtgat ggccaccgcg tcggcccgct cggtggtacg
      541 aacgctgacg cgggtggtct ccgcatcggc ctccttgaac aggatggtga tgtcggcggc
      601 acgggtcgag cccagcaggt ccacgaagcc ctcgctggcg gtcggctcga cgccgatctc
      661 ggccagcatg gccagcgtca tcgaggcgtg gacgatgcga ccctcgcagc gctgctggat
      721 ggcggccagc atgcgacccc acagcgccaa cgtggtgaac ggcttgtcgg cgtagaccgc
      781 gcggttgatg gccgatagcg gcgcaccggc ctccaccagc tgggcggcca cccgcagggt
      841 ccgtggggtg gcattcggat gggcgaaggt gcgcgtgtcc tggacgatgc ccgccatcag
      901 caccgtcgcc agctccgtgt ccagctccac gccaagctcg ggcaacagca aggcgatcat
      961 ctcgcaggtg gcggccgcct cggcatccac ccagtccacg ctgccgaagc gcgtgttcga
     1021 gacgtggtgg tcgatattga cgatggtcgc ccgcgacagc cagtccgcgt gctcggcggc
     1081 cacgccgccg gtgcgcgata ggtcgccgga gtccaccacg accgcaacgt ccggctccag
     1141 ctgcggggcg cggcggacgg tctcgacgcg cgggaggaag gccaggaagg gcggcggcgg
     1201 gtcggcgctc accacctcgg cctgcttgcc aagccgctcg gcggcgatgg cgatggcgat
     1261 ggcggcccct agtgtgtcgg cgtccgggtt ctcgtggcat accgtggtga tgcgctgcgc
     1321 cgccaggatg cgctcagtgg cctccttcat cggtcgtccg tcccccgctc ctgctcgagc
     1381 tcctggagca ggcgcagcac gcggtcgccg gcctccaccg aatcgtcgcg gcgcgacgtc
     1441 agctcgggga cgtgccggat ccgaagccgc tcggccaggc gccggcgcag ccagggagtg
     1501 gcgtccgtca gggcgtccag cgagcgctgg cgatcctcat cggtcccgta cacgctcacc
     1561 cagacccggg catggccaag gtcgcgagcc gtctccaccc gagtgatggt ggcgaacccc
     1621 acgcgcggat ccttcatctc gcgctggagc aggtccatca gctcctgctg gatctggctg
     1681 tccaggcggt cggtacgctg gctcacgacg gctcaggcct gctcggccac catctggtag
     1741 cactcgatca cgtcgccctc ggcgatgtca tcgatggcca gcccgatgcc gcactcgaag
     1801 ccggtcgcca cctcgcgcac gtcgtccttg aagcgccgca gggactcgat ccggctctcg
     1861 gcgatctgct tgcccccacg gaagagccgg gcctggccgc cgcgcacgat gcgcccatcg
     1921 gtcacgtacg agccggccac gttcccggcg cgcccggcct tgatcaccat gcgcacctcg
     1981 gcatggccct ccacctgctc gaccagcttg ggcgtcagca tgcccttgac ggcggtgccc
     2041 aggtcgtcgg tgagcttgta gatgacgtcg tagatgcgca cgtccacgcc gctgctgtcg
     2101 atcacgcgtt gcgcgccggc atccaccctg gtgttgaagc cgatgaccac cgcctccgag
     2161 gccgcggcca gcagcacgtc cgactcgttg atgtcgccag cgccctcgcg caggatgctg
     2221 atccgcacct cggcggtgtt caaccgctcc agcgcgtgcc ggatggcgcc cagcgagccc
     2281 tggacg
//
LOCUS       DATACA010000390         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_774077, whole genome shotgun sequence.
ACCESSION   DATACA010000390 DATACA010000000
VERSION     DATACA010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774077"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VHK28_08070"
     CDS             complement(<1..1377)
                     /locus_tag="VHK28_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142208.1"
                     /translation="MAARTRARLSLQSYGEGVDRFSASYLEALYRQLAGLDETMGLPA
                     IYAEHASLFETSAIDALRGLADGQDDEAAQARLFLEFAVTEHLSAQVAELTDRIETAE
                     AQAVIIWRGERIPYRAAWNRAADISGRAERNGLADSYHEAVEAINPLREERLEKRAEA
                     VRGLGYRDVTDMVQQTAGFDPQQLAAELRAFLVDSETVYFAALRRNLAEIDIEQGDAS
                     QVDLAHVLRGAGWDAWFDARGMVPALRRTLATLGIDMEAQGNITLDVEARPRKSPRAF
                     CAPVSVPDDIRLVIQPRGGWDDYAALLHEAGHAQHFSHVSADLPAAFRRLGDNSLTEG
                     YGVLFERLVGDPGWLMDHTGMAEADALAFADFNAFWYLRLVRATAAQFMYELVLHSGV
                     EPAVAREQYVGMRSLTEGVTFAPAGYLQLDDDLYSARYARATMLEGSLSAWLRHRHGE
                     SWWRSAEAG"
BASE COUNT          235 a          581 c          567 g          237 t
ORIGIN      
        1 cccggcctcg gccgaccgcc accacgattc accgtgccgg tgtcgcagcc atgccgagag
       61 gcttccctcc agcatggtgg cccgcgcgta gcgagcggag tacaggtcgt cgtccagctg
      121 caggtagccg gccggcgcaa aggtgacccc ctcggtcagg ctccgcatcc cgacgtactg
      181 ttcgcgggcc acggccggct ccacgcccga atgcagaacc agctcgtaca tgaattgcgc
      241 ggcggtggcc cgcaccaggc gcaggtacca gaaagcgttg aagtctgcga aggccagcgc
      301 atctgcctcg gccatgccgg tgtggtccat cagccagccg gggtcgccga cgagccgctc
      361 gaagagcacg ccgtagccct cggtcaggct gttgtcgccc agccggcgga aggcagccgg
      421 caggtcggcc gagacatggc tgaaatgctg ggcgtgacca gcctcgtgca ggagggcggc
      481 atagtcgtcc cagccgccgc ggggctggat caccaggcgg atgtcatccg ggaccgatac
      541 cggggcgcag aacgcgcgcg gcgacttgcg cggccgcgcc tcgacgtcga gggtgatgtt
      601 gccctgcgcc tccatgtcga tgcccagcgt ggccagcgtg cggcgcaggg ccgggaccat
      661 gcctcgcgcg tcgaaccagg catcccaccc cgcaccacgg aggacgtggg ccaggtccac
      721 ctggctggcg tccccctgct cgatgtcgat ctccgccagg ttgcgacgca gcgcggcgaa
      781 gtacaccgtc tccgagtcga ccaggaaggc gcgcagctcg gcggcgagct gctgcggatc
      841 gaatccggcc gtctgctgca ccatgtcggt gacgtcgcgg tagcccaggc cacgcaccgc
      901 ctcggcccgc ttctcgagcc gctcctcgcg cagggggttg atggcttcca ccgcctcgtg
      961 gtacgagtcg gccaggccgt tgcgctcggc gcggccgctg atgtccgcgg cgcggttcca
     1021 ggcggcacgg tacgggatgc gctccccgcg ccagatgatg accgcctgcg cctcagcggt
     1081 ctcgatccga tccgtcagct ccgcaacctg ggcggagaga tgctcggtga ccgcgaattc
     1141 gaggaagagc cgggcctggg ccgcctcgtc gtcctgaccg tccgccaggc cgcgcagcgc
     1201 gtcgatggca ctcgtctcga agagcgaggc gtgctcggcg tagatggcgg gcaggcccat
     1261 cgtctcgtcc aagcccgcca gctgtcggta aagcgcctcc aggtagctgg cgctgaaccg
     1321 atccactccc tcgccgtagg actgcaggct caaccgggcg cgcgtgcggg ctgccatgcg
     1381 gttggtcagc gggttccctt ggccacggga gcgcccggtg ccttcgccgt gccatcttcc
     1441 gacgccgcca gcgccagccc cgcgtgccgg gccgcggctc cacgaagtca cggaagaggg
     1501 ggtgcggccg gttgggccgg ctcttcagct cggggtgggc ctgggtggcc acgaaccacg
     1561 gatggtcgcg cagctcgatg tactccacca gccgcccatc gggcgatacc ccgctgaacc
//
LOCUS       DATACA010000391         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2541507, whole genome shotgun sequence.
ACCESSION   DATACA010000391 DATACA010000000
VERSION     DATACA010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541507"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..870
                     /locus_tag="VHK28_08075"
     CDS             115..870
                     /locus_tag="VHK28_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX2142209.1"
                     /translation="MTDTGAATARPRLTFFFPAYNEEENVEATVAQALEKIGPLVDGS
                     LEVLIVDDGSSDRTPQLADALAAADPRVRVHHQPNRGYGGALKAGFANARGELVCFSD
                     GDLQFDLAEMSRLLSRLEDGSRGPVDAVIGYRIKRRDPPHRIFIAKTYNAIVSVAFGL
                     RVRDIDCAMELFRREVFDGLRLDADGPFLSAELLIKLRALGVRMAQVGVNHYPRAAGT
                     NTGASFLKILRTFRDLAVLRLALWTRREEVLRA"
     gene            complement(1646..2197)
                     /locus_tag="VHK28_08080"
     CDS             complement(1646..2197)
                     /locus_tag="VHK28_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX2142210.1"
                     /translation="MAEGPFGRVRLIEMVVESVRVHMLSSQHVVILKESERDRYLPIW
                     IGPSEANAIAMRLQGLSAERPLTHDLMVSVIASLNSSVSRVVVTHVTDGTFHARLYLE
                     AADGSEAEIDSRTSDAIAVAVRTGSTIYVDERVLDEAGVEPDREDGEPSEERLAVFRE
                     FVNSLDVDLEGGEKGPPAEEQGQ"
     gene            complement(2214..>2498)
                     /locus_tag="VHK28_08085"
     CDS             complement(2214..>2498)
                     /locus_tag="VHK28_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142211.1"
                     /translation="PPDADVRDPLDYRLLDGATIGDRELFYVYDAANARIVGYQRADG
                     AFVRQWMAPRSGDAAGVLDDVVAMSVASVTDGPPAALLLTPEGVIRVILE"
BASE COUNT          390 a          865 c          854 g          389 t
ORIGIN      
        1 cgtctacctg gtgccacccc actggctgct ggagcgcatc gagcgccggt gggaggcaga
       61 gaccgttccg acctacgcgg gctacaccga cctgctccgc atcgagggac ctcaatgacc
      121 gatacgggcg ccgcgaccgc ccggccccgg ctgaccttct tcttccccgc ttacaacgag
      181 gaggagaacg tggaggccac ggtggcccag gcgctggaga agatcgggcc gctggtggac
      241 ggctcgctgg aggtgctgat cgtggacgac ggctcgtcag accgcactcc acagctggcc
      301 gatgcgctgg ccgccgccga cccgcgcgtc cgggtccatc accaaccgaa ccgcggctac
      361 ggcggcgcgc tgaaggccgg cttcgccaac gcccgcggcg agctcgtctg cttctcggac
      421 ggcgacctgc agttcgatct ggccgagatg tcgcgcctgc tgtcccggct cgaggacggc
      481 tcgcggggcc cggtggacgc ggtgatcggg taccgcatca agcgccgcga cccgccgcat
      541 cggatcttca tcgccaagac ctacaacgcc atcgtctcgg tggcctttgg cctgcgggtg
      601 cgcgacatcg actgcgccat ggagctcttc aggcgcgagg tgtttgacgg cctgcggctg
      661 gacgcggacg gacccttcct ttcggccgag ctgctgatca agctgcgggc gctgggggtg
      721 cggatggccc aggtcggcgt caaccactac ccgcgcgcgg cgggcaccaa caccggtgcc
      781 tcgttcctga agatcctgcg cacgttccgg gacctggccg tgctgcgcct ggccctgtgg
      841 acgcgacgcg aagaggtgct gcgggcctga gcccggagcg gctggcgcgg cgaccctgcc
      901 agaagtggcg gtgcgaccgg cttagccgtc cggttggccc cgattccctg tcagagcccg
      961 ccggaaggtg gacttagcca gaaagatcac gctcagtacc tgcgcccggc gggcggcaac
     1021 ctggcttaag cccccttggc gccggcctta ggcaggaaag ccgcgccaac attcggcata
     1081 agcccgcccc agccccggat tcggccaaga atcaacccgg ccccggttga ggcaaggtcc
     1141 gctgctgggt aggtacggca ggaacgcgac gatcctcctg gcgacgcggc cccgcgagcc
     1201 gctttcgtgt ggaaatgcgg taccggtgcc gcctagcgcc cacgaagttg gccgaccagt
     1261 gcgccatcgt cgctctcagg ccgcacccac atgacatttc ccaacgaaag ggtgcttgaa
     1321 acccggatct acggggcttc ctggacgaac tgcctggccg gcgatgcgcc gcgccgtgct
     1381 tcagtgccac tacctggtca tcggtccgat tggtgcgcgt tacctcgcta gctggccaat
     1441 cttcttgatc agaagctgcc ccggtgcgcc tgggaaaggt agctcgtgct cagcggggcc
     1501 gcctcaggcc gcgtcacgct cgccggagct gggctaaggg tcgccaggcg agcggtagac
     1561 gaagaagact gaccaactgg accgataagg ctcaccagat gagcagctgg ccaggttggc
     1621 gacgcaccgc cccgcggcgc ccgctctact ggccctgctc ttcggccgga ggccccttct
     1681 cgccgccctc caggtccacg tcgagcgagt tgacgaactc gcggaagacg gcgagacgct
     1741 cctcggacgg ttccccgtcc tcgcggtccg gctccacgcc ggcctcgtcg agcacccgct
     1801 catcgacgta gatggtggac ccggtgcgca ccgcaaccgc gatcgcgtcg gaggtgcggg
     1861 aatcgatctc cgcttcggag ccgtccgcgg cctccaggta gaggcgggcg tggaaggtgc
     1921 catcggtcac gtgggtgacc accacccgcg acacgctcga gttcaggctg gcgatcacgc
     1981 tgaccatcag gtcgtgggtc agcgggcgct ccgcgctcag gccctgcagg cgcatggcga
     2041 tggcgttggc ctcggatgga ccgatccaga tagggaggta tcggtcccgc tcggactcct
     2101 tgaggatgac cacgtgctgg ctcgagagca tgtgcacccg cacgctctcc accaccatct
     2161 cgatcagccg gacccggccg aacggacctt cagccatgga tcgattctag aggctactcg
     2221 aggatgacgc ggatgacgcc ctcgggggtg aggagcaggg ccgcgggtgg gccatcggtg
     2281 accgatgcca cgctcatggc caccacatcg tccagcacgc cggcggcgtc accgctgcgc
     2341 ggagccatcc actggcggac gaaggcgccg tccgcgcgct ggtagccgac gatgcgggcg
     2401 ttggccgcgt cgtagacgta gaacagctcc cggtcgccga tggttgcgcc gtccagcagc
     2461 cggtagtcga gcgggtcgcg gacgtcggcg tccggtgg
//
LOCUS       DATACA010000392         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1257027, whole genome shotgun sequence.
ACCESSION   DATACA010000392 DATACA010000000
VERSION     DATACA010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257027"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /gene="dnaE"
                     /locus_tag="VHK28_08090"
     CDS             complement(<1..1467)
                     /gene="dnaE"
                     /locus_tag="VHK28_08090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX2142212.1"
                     /translation="MTLRPEKSPADDFVHLHVHSEFSLLDGLSRSRELTQRAAELGMS
                     ALAVTDHGAMYGAIEFYQSAIAAGIKPILGIEQYVAPRRMTDKEGKTDADYHHMVLLA
                     KNAVGYRNLLALTTAAHLDGYYYKPRIDKELLARHSEGLIGTSACLGGEVLRRLAAGD
                     ERGARQAADDYRSILGPENFFIEVQDHGVPEQNRLHPQLVELARDMKIRLLATNDTHY
                     TVPEQHEAHDLLLCIGTGSNVDTPGRLRFETNEFFLKSPQQMRTLFRGELAEAMDNTL
                     RVAEMVDLRLEFDQLRLPHFPVPEGETAASWLRKECERGLVDRYPRVTDEIRHRLDYE
                     LGIIDRMGYSAYFLIVADFTRFAREQGIFTTCRGSAPGSIVTYSLGITPVDPLAYGLP
                     FERFLNPDRVTMPDIDIDFQDSRRDEVIEYVTRKYGDDRVAQIITFGTLGAKAAIRDV
                     GRTMGLTYAEADRVAKAVPNELAISLDRAVETSPQLREL"
     gene            complement(1498..>1919)
                     /locus_tag="VHK28_08095"
     CDS             complement(1498..>1919)
                     /locus_tag="VHK28_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX2142213.1"
                     /translation="YWKSEFVPGISDGLLETYNAQFVDQKAPANQIVLFHVGGALNEL
                     AEDDGAVGNRDAAFACVVQSMGKPDAPHEENRAWVRSAWEALRQYGTGGNYINFQTDD
                     EPDARTEGSYRSNFDRLRAAKRHFDPANVFRVNRNIR"
BASE COUNT          287 a          637 c          655 g          340 t
ORIGIN      
        1 cagctcgcgc agctgcggcg acgtctccac ggcgcggtcc agggagatgg ccagctcgtt
       61 ggggaccgcc ttggcaaccc ggtccgcctc ggcgtaggtg aggcccatgg tgcggcccac
      121 gtcgcggatg gccgccttgg cacccagcgt cccgaaggtg atgatctggg ccacccggtc
      181 gtcgccgtac ttgcgggtga cgtactcgat cacctcgtcc cgccgtgagt cctggaagtc
      241 gatgtcgata tccggcatcg tcacccggtc cgggttgagg aaacgctcga acggcagccc
      301 gtaggccagc gggtcgaccg gcgtgatgcc cagcgagtag gtcacgatcg accccggcgc
      361 cgaaccccgg caggtggtga agatcccctg ctcgcgcgcg aagcgggtga agtcggcgac
      421 gatcaggaag taggcgctgt accccatccg atcgatgatc cccagctcgt agtccaaccg
      481 gtggcggatc tcatcggtca cgcgggggta tcggtccacc aaaccgcgct cgcactcctt
      541 gcgcagccag gaggcggcgg tctcgccctc cggcaccggg aagtggggca ggcgcagctg
      601 gtcgaactcg aggcggaggt ccaccatctc ggccacgcgc agcgtgttgt ccatggcctc
      661 ggccagctcg ccccggaaga gcgtccgcat ctgctgcggc gacttcagga aaaactcgtt
      721 cgtctcaaag cgcagccgtc cgggcgtgtc cacgttgctg ccggtcccga tgcacaacag
      781 cagatcgtgc gcctcgtgct gctccggcac cgtgtagtgg gtgtcgttgg tggccagcag
      841 ccggatcttc atgtcacgcg ccagctccac cagctggggg tgcagccggt tctgctccgg
      901 gacaccgtgg tcctgcacct ctatgaagaa gttctccgga cccaggatgg accgatagtc
      961 atcggcggcc tggcgtgcgc ctcgctcgtc gcctgcggcg aggcggcgca gcacttcccc
     1021 gcccaggcag gcggaggtac cgatgagccc ctcggagtgc ctcgccagca gctccttgtc
     1081 gatgcgcggc ttgtagtagt agccgtccag gtgcgcggcc gtggtcaggg ccagcaggtt
     1141 gcggtagccg acggcgttct tcgccagcag caccatgtgg tggtagtccg cgtccgtctt
     1201 gccctccttg tcggtcatgc gccgcggggc gacgtactgc tcgatgccga ggatgggctt
     1261 gatgcctgcc gcgatcgccg actggtagaa ctcgatggcc ccgtacatcg ccccgtggtc
     1321 ggtcacggcc agggcggaca tgcccaactc ggcggcacgc tgtgtcagct cgcggctgcg
     1381 ggagaggccg tccaggaggc tgaactcgga gtggacgtgg aggtggacga aatcgtccgc
     1441 ggggcttttc tcgggtcgca gggtcaccca gccatggtgc cagaactggt gaccgcgtca
     1501 gcggatgttt cggttcaccc ggaacacgtt cgcggggtcg aaatgccgct tcgcggctcg
     1561 cagccggtcg aagttggacc ggtacgagcc ttcggtgcgg gcatcgggct cgtcatcggt
     1621 ctggaagttg atgtagttgc ctccggtccc gtactggcgg agcgcttccc aggccgagcg
     1681 cacccacgcg cgattctcct cgtgcggcgc atcgggcttc cccatcgact gcaccacgca
     1741 ggcgaaggcg gcgtcccgat tgccaactgc accgtcgtcc tccgcaagct cgttgagcgc
     1801 gccgccgacg tggaacagca cgatctggtt ggccggcgcc ttctggtcga cgaactgggc
     1861 gttgtacgtc tccagcagcc cgtcgctgat gccgggcacg aactccgatt tccagtagt
//
LOCUS       DATACA010000393         2906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1175976, whole genome shotgun sequence.
ACCESSION   DATACA010000393 DATACA010000000
VERSION     DATACA010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175976"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VHK28_08100"
     CDS             complement(<1..1268)
                     /locus_tag="VHK28_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX2142214.1"
                     /translation="MAVWPRLDDLARLPLPGSAAPGSIAFAPDGSAITYLHSTDASLV
                     RALWWHDLESGERRMIAGPPAEATTEESLGLEEQLRRERTRTSELGVTQYAWAREAAV
                     PTLVVPVGGQVYVAVGNDVRRGVTALEGADGVASAVVSPDGRSVAFVRDGELWICGVA
                     GGAPRRLTGDAGDGVFNGLAEFAAAEELDRHEGLWWSADSAAIAYAHVDERHVPPFVI
                     PHLGAGPGEPPSEQHRYPFAGGPNARVTLRISEAAGGSHQELDLGMAPDDYLARVVAE
                     PGGGWLVAVLPRDQRSLRWLRVGRDGAARELWVERSEPWLNLDNHTRVLRDGRVLRST
                     ERTGYRHLELRTADGPLERQLTGGEWVVTDVAHVDEDRGEVLFLATRHGGTERHLYAA
                     PLDAPEPVADPQRLTEDPGWHQVSVSRDGSA"
     gene            complement(1274..1780)
                     /locus_tag="VHK28_08105"
     CDS             complement(1274..1780)
                     /locus_tag="VHK28_08105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX2142215.1"
                     /translation="MADRPMPPSGALDTSKSYTATFKTEKGDFSVELYPAEAPRTVEN
                     FVNLARSGFYDGTTFHRVIGGFMAQGGDPTGTGTGGPGYSFADEFHPSRRHDAAGVLS
                     MANAGANTNGSQFFITFGPTPHLDDRHTVFGRVVKGMEVVRSLRERDPSRDRNPGDRI
                     ESIEISES"
     gene            complement(1852..2490)
                     /locus_tag="VHK28_08110"
     CDS             complement(1852..2490)
                     /locus_tag="VHK28_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142216.1"
                     /translation="MIVPAFRRTVLLSLVTLALAACTASSPSASVPAEPSPSADVASA
                     EPSSEPSAPASQSAAPSADPSEDLGEFACELPVSGVGNLGLAHLLDVRVGEHEDFDRI
                     VFEFDEGIPRFTLESAEPPFTEDASGLPLEVEGNQFLSLRLEGGTKQGEGGEATYDGP
                     TEFEPDFSALVHLVEGGDFEAQSTWYIGMESDACMRVTSLPAPNRLVIDIQH"
     gene            complement(2565..>2906)
                     /locus_tag="VHK28_08115"
     CDS             complement(2565..>2906)
                     /locus_tag="VHK28_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013860942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX2142217.1"
                     /translation="AAIVAVHASATTYAETDWREIVALYDALYRLQPTPIVALNRALA
                     LAEVAGPAAALAAVEPLAERLDRYHLFHAARGDLLGRLGRAEDARAANARALELTTNP
                     AERRLLLERMA"
BASE COUNT          392 a         1043 c         1070 g          401 t
ORIGIN      
        1 gcgctgccgt cgcggctgac ggaaacctgg tgccagcccg gatcctcggt cagccgctgg
       61 ggatccgcga ccggttccgg agcgtccagc ggcgcggcat acaggtgccg ctcggtcccg
      121 ccgtggcggg tggccaggaa gagcacttcg ccgcggtcct cgtcgacgtg cgcgacgtcg
      181 gtgaccaccc actccccgcc ggtgagctgc cgctccagcg ggccgtcggc cgtccgcagc
      241 tccaggtggc ggtacccggt ccgctccgtg ctgcgcagga cgcggccgtc gcgcaggacg
      301 cgggtgtggt tgtccaggtt gagccacggc tcggaacgct ccacccacag ctcgcgcgcc
      361 gcgccgtccc gccccacgcg cagccagcgc agcgagcgct ggtcgcgggg caggacggcg
      421 accagccagc caccgccggg ttcggcgacg acgcgcgcca ggtagtcatc cggcgccatg
      481 cccaggtcca gctcttgatg cgagccacca gcggcctcgc tgatccgcag cgtgacccgg
      541 gcattcgggc cgccggcgaa cgggtagcga tgctgctcgc tgggtggctc gccgggcccg
      601 gcgccgaggt gggggatgac gaacggcggc acgtggcgct cgtccacgtg ggcgtaggcg
      661 atcgccgcgc tgtcggccga ccaccacagc ccctcgtggc ggtcgagctc ctcggcggcg
      721 gcaaactcgg ccaggccgtt gaagaccccg tcgcccgcgt cgccggtcaa ccgccgcggc
      781 gcgcctcccg ccacgccaca aatccacagc tcgccgtcgc ggacgaaggc gaccgaacgc
      841 ccgtcaggcg agacgaccgc ggaggccacg ccatcggcgc cctccagggc ggtcacgccg
      901 cgccgcacgt cattaccgac ggccacgtac acctggccgc ccaccgggac caccagcgtc
      961 gggacagccg cttctcgggc ccaggcgtac tgggtcacgc ccagctcgct ggtccgcgtc
     1021 cgctcgcggc gcagctgctc ctcgagcccc agggattcct cggtggtggc ctcggcgggc
     1081 ggcccggcga tcatgcgccg ctcgccgctc tccaggtcgt gccaccacag cgcgcgcacc
     1141 agggaggcat cggtgctgtg caggtaggtg atggccgacc cgtccggggc gaacgcgatc
     1201 gaacccggag ccgcgctgcc cggcaggggc aggcgcgcca ggtcgtccag ccgcggccag
     1261 accgccacct gggtcaggac tcggagatct cgatcgactc gattcgatcg cccgggtttc
     1321 ggtcgcgcga gggatcgcgc tcccgcagcg agcggaccac ctccatgccc ttcaccacgc
     1381 ggccgaagac ggtgtggcgg tcatcgaggt gcggcgtcgg gccgaaggtg atgaagaact
     1441 ggctgccgtt ggtgttcgcg cctgcgttgg ccatggacag cacgccggca gcgtcgtggc
     1501 ggcggctggg atggaactcg tcggcgaagc tgtagccggg accgccggtg ccggtgccgg
     1561 tcgggtcccc gccctgggcc atgaagccgc cgatcacgcg atggaacgtc gtgccgtcgt
     1621 agaacccgga gcgggccagg ttgacgaagt tctccaccgt gcgcggagcc tcggcgggat
     1681 acagctcgac gctgaagtcg cccttctccg tcttgaaggt ggcggtgtag gacttagagg
     1741 tgtcgagggc gcccgagggc ggcatcggtc ggtcggccat tgggtctccg cgggtacgga
     1801 cttggaccgg accgatgcta ccgggtgtgc cgcccgcgcg cccgcacggg ctcagtgctg
     1861 gatgtcgatc accagccggt tcggcgcggg caggctggtg acgcgcatgc aggcgtccga
     1921 ctccatgccg atgtaccagg tgctctgcgc ctcgaagtct ccgccctcca ccaggtggac
     1981 gagcgcactg aagtccggct cgaactcggt cggaccatcg taggtggcct cacctccctc
     2041 gccctgcttg gtgccgccct ccaggcgcag gctcaaaaac tggttgccct cgacctccag
     2101 cggcaggccg ctggcatcct cggtgaaggg cggctcggcg ctctccagcg tgaagcgggg
     2161 gatgccctcg tcgaactcga agacgatgcg gtcgaagtcc tcgtgctcgc ccacccggac
     2221 gtcgagcagg tgggccagcc ccaggttgcc cacgccgctg acaggcagct cgcaggcgaa
     2281 ctcgcccagg tcctcgctcg gatcggcgga cggtgccgcc gactggctgg ccggagcgct
     2341 cggctccgac gacggctcgg cgctggccac gtccgctgac ggcgacggct cggccgggac
     2401 cgatgcgctg gggctggagg cggtgcaggc ggcgagcgcc agcgtcacca gcgacaggag
     2461 gacggttcgc cgaaaggcgg ggacgatcac gtggcttgct ccgtacatgt gacgcccggc
     2521 ctgatcgccg ggcatcggtc aatacgcgcg gggggagcgg cctgttacgc catgcgctcc
     2581 agcagcagcc gccgctcggc cgggttggtg gtcagctcca gggctcgtgc gttggcggcc
     2641 cgcgcgtcct cggctcggcc caggcgccca aggaggtcgc cgcgggcggc gtggaagagg
     2701 tggtatcggt ccagccgttc ggcgagcggc tcgaccgccg ccagggccgc ggccggcccg
     2761 gccacctcgg cgagggccag cgcccggttg agcgccacga tcggcgtggg ctgcaagcgg
     2821 tacagcgcat cgtagagcgc cacgatctcc cgccagtcgg tctcggcata ggtggtggcg
     2881 gatgcgtgca cggcgacgat ggcggc
//
LOCUS       DATACA010000394         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2863682, whole genome shotgun sequence.
ACCESSION   DATACA010000394 DATACA010000000
VERSION     DATACA010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2863682"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="aspS"
                     /locus_tag="VHK28_08120"
     CDS             complement(<1..1137)
                     /gene="aspS"
                     /locus_tag="VHK28_08120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX2142218.1"
                     /translation="MGQSFRTHTCGELRAEHIGERVTLAGWVHRRRDHGHLTFLDLRD
                     RYGITQVVTSAEDAPDAHAAADPVRNEWVIQVEGVVRNRPEGTANAELPTGAIEVGVD
                     RLAVLNPSKVPPFYVNEEQPGLDESLRLKHRYLDLRRPPLQQRILLRSRLASAIRRAL
                     EDEGFVEIETPTLIRSTPEGARDFVVPSRLRPGEFYALPQSPQQLKQLLMVAGYDRYF
                     QLAHAYRDEDLRGDRQPEHTQVDIEMSFVREEDVMATVEVMVTRVTREVLPKRPILAS
                     PFPRFSYEEAIARFGSDKPDVRFGMELVDLTDALRGLDFRVFAEPIAAGGAVYAIRAP
                     GCGDYSRKQLDELIDLAQRHGARGLAHLAVEMDGSLRGPAAKFLG"
     gene            1416..1865
                     /locus_tag="VHK28_08125"
     CDS             1416..1865
                     /locus_tag="VHK28_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142219.1"
                     /translation="MHIPLPWAIALTTAGAMLVVTLLGLGVRAGRIRAPERALFHVAY
                     LLGGGVTGFLALAFPPAWPFLAFIAGVLGLKSLRERRLTDFGLMLCGFGAAWTLLLGA
                     SMINDISDPAVHGSPGVLTWFLAGSGVLVAGVLVLIRHAARPSGRTC"
     gene            complement(1898..>2217)
                     /locus_tag="VHK28_08130"
     CDS             complement(1898..>2217)
                     /locus_tag="VHK28_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142220.1"
                     /translation="RRGVGTRNHCVHGEGASLEEVLDWRPHEYFTVRSVAPGLATWVA
                     MEELTPTEMGTHLTIRLQRPRAAKDRSALEERRDMILGLYQHGLDRVREEFSRHEEPA
                     GIG"
BASE COUNT          304 a          758 c          781 g          374 t
ORIGIN      
        1 acccaggaac ttggctgccg ggccgcgcag tgagccgtcc atctccaccg ccaggtgggc
       61 caggcccctg gcaccgtggc gctgtgcgag gtcgatgagc tcgtcgagct gcttgcgcga
      121 gtagtcgccg cagcccggcg cgcggatggc gtacaccgcg ccaccggcgg cgatcggctc
      181 ggcgaagacg cggaagtcga ggccacgcag ggcatcggtc aggtccacca gctccatgcc
      241 gaagcgaacg tcgggcttat ccgagccgaa gcgagcgatc gcctcctcgt agctgaagcg
      301 cgggaagggg ctggccagga tcggccgctt gggaagcacc tcgcgcgtca cgcgggtgac
      361 catgacttcc accgtggcca tcacgtcctc ctcgcggacg aagctcatct cgatgtccac
      421 ctgggtgtgc tctggctgcc gatcgccgcg caggtcctcg tcgcggtagg catgggccag
      481 ctggaagtag cggtcgtagc cggccaccat caacagctgc ttcagctgct gcggcgactg
      541 cggcagcgcg tagaactcgc ccgggcgcag ccgtgaggga accacgaagt cgcgggcgcc
      601 ttccggcgtg gagcggatga gggtgggggt ctcgatctcc acgaagccct cgtcctccag
      661 cgctcgccgg atagcggacg ccaggcgcga gcgcagcagg atgcgctgct gcagcggagg
      721 gcggcgcagg tccaggtacc gatgcttcag ccgcagcgac tcgtccaggc cgggctgctc
      781 ctcgttgacg tagaagggcg gcaccttgct ggggttgagg acggcgaggc ggtccacccc
      841 gacctcgatg gcgccggtcg gcagctcggc gttggccgtg ccctccggtc ggttacgcac
      901 cacgccctcg acctggatca cccactcgtt gcgcaccggg tcggccgcgg cgtgggcgtc
      961 cggggcgtcc tcggcggagg tcaccacctg ggtgatcccg taccggtccc gcaggtcgag
     1021 gaaggtgagg tgtccgtggt cgcgccgacg gtggacccag ccggccagcg tcacccgctc
     1081 accgatgtgc tcggcgcgca gctcgccgca ggtgtgcgtg cggaaggact gacccatggg
     1141 acgcgaatgg tagccgagcg agcgcggcgg gaccggtacc cgacaggtga cgcaggattt
     1201 cacacgaggg ggcggctgcg tgctgattcg ctggtacatc gggcaggcct cagcacgagc
     1261 cgacaagttc tcgtcccttc acgctcaccg ctgcccgaca tatcggcaga acgacaggca
     1321 gatcacgcct cgcgtgaagt tctgcccgat attttggcgc cgccgcacgg tgccttaccc
     1381 ccggaagttg ctgctcgttc atatcctgta tcgcaatgca catcccgctc ccctgggcga
     1441 tcgcgctgac gacggcgggc gccatgctgg tggtcacgct gctgggcctc ggcgtccggg
     1501 cgggtagaat ccgcgcgccc gagcgagccc tgttccacgt ggcctacctg ctgggcggcg
     1561 gcgtgaccgg gttcctggcc ctggcttttc cgccggcgtg gccgttcctg gccttcattg
     1621 ctggcgtgct cggcctcaag tcactgcgcg agcgacgcct caccgatttc gggctgatgc
     1681 tctgcggttt cggggctgcg tggaccctct tgctcggcgc gagcatgatc aacgacatct
     1741 ccgatccggc ggtccacggg tcccctggcg tgctcacctg gttcctggcg ggttccggcg
     1801 tcctggtggc aggcgtgctg gtgctgatcc ggcatgccgc gcggccatcc ggtcggacct
     1861 gttgaaagcg atggcctgag ggcgtcggct ggcgcgatca gccgattcct gccggttcct
     1921 cgtgccggga gaactcctcg cggacgcggt ccagcccgtg ctggtagagc ccgaggatca
     1981 tgtctcggcg ctcctcgagc gcgcttctgt ccttcgccgc gcgcggacgc tggagccgga
     2041 tggtcaggtg cgtacccatc tcggtggggg tcagctcctc catggccacc caggtcgcca
     2101 gcccgggcgc cacgctgcgg acggtgaagt attcatgcgg ccgccaatcg agcacctcct
     2161 cgagcgaggc gccttcgcca tgtacgcagt ggttgcgggt gcccactccc cggcgcc
//
LOCUS       DATACA010000395         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_694406, whole genome shotgun sequence.
ACCESSION   DATACA010000395 DATACA010000000
VERSION     DATACA010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694406"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VHK28_08135"
     CDS             complement(<1..592)
                     /locus_tag="VHK28_08135"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050825 - ice binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HEX2142221.1"
                     /translation="MAAEQPTMGTAANYAVMATSTITNTGATTIIGGLALDPGTAVTG
                     APVVVGSYDVDNAAAELAHDHLIVAYDEAFNATTTTVLTGSDLGTVGVLTPGVYTFDS
                     SAQLTGNLTLDGGGATNPVFIFQMGSTLTTAPAASVTLQNGAGACAIFWQVGSSATID
                     AGTSFQGTIMAAESITMNAGATIGAGGLNYGGRALAM"
     gene            1241..1927
                     /locus_tag="VHK28_08140"
     CDS             1241..1927
                     /locus_tag="VHK28_08140"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing protein"
                     /protein_id="HEX2142222.1"
                     /translation="MPSSVPQLRVPVVAIDPVIPADELVARPSANEREAATAARATAT
                     AAPSAAPSRLVALAPLTIDVWNADERYFSVSGSTPDEIVASAVANVPPDPSGAARSTM
                     AYVGPVTWNHRPSYVQDPATGSCTMTAVASTVAYQATLPQWTSPSSVQPELVAWWHLV
                     LDHVRAHESEHVRIYEGFVITLPDRVVGQPCAEWDGIIGKWIADLTVAQSAFDAAESH
                     WSLPVYTPGE"
     gene            complement(2194..>2492)
                     /locus_tag="VHK28_08145"
     CDS             complement(2194..>2492)
                     /locus_tag="VHK28_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142223.1"
                     /translation="QPPSPADRDPSAELEARIAELERRSWRQTVDEGRSGMEEAFWAL
                     MHRLFPEEARKHLKTAGREQLMAARVYLDKWIERLDEEEQAATAARPHEKIEIE"
BASE COUNT          393 a          837 c          846 g          416 t
ORIGIN      
        1 tcatggccag tgcgcggccg ccatagttga ggccgccagc accgatcgtg gcaccggcat
       61 tcatggtgat gctctccgcg gccatgatgg tgccctggaa ggaggtcccc gcgtcaatcg
      121 tggcggagct gccgacctgc cagaagatcg cgcaggcgcc agccccgttc tggagtgtga
      181 cgctggcggc cggagcggtg gtcagggtgc tgcccatctg aaagatgaat accggattcg
      241 tagcgccgcc accgtcaagg gtcaggttgc cggtcagctg agccgagctg tcgaaggtgt
      301 agacaccggg ggtcagcacg cccaccgtac cgagatcgga gccggtaagg acggtggtgg
      361 tggtggcgtt gaacgcttcg tcgtaggcga cgatcaggtg atcgtgggcc agctcggcgg
      421 cagcgttgtc gacgtcgtag ctgccgacaa ccaccggcgc gccagtgacc gcggtaccgg
      481 gatccaatgc caggccgccg atgatcgtgg tggctccggt gttggtgatg gtgctggtcg
      541 ccatgaccgc gtaattcgcg gcggttccca tggtcggctg ctcggcagcc atggtcacgg
      601 tggacgtgaa cgccagtgcg caagccaccg cgacggcgga gataagacgg atcggtcgag
      661 tgaaggtcaa gcgacgcatg tgcgacaccg gtaagggtcg agcgtcgtgg ccgatggtta
      721 tcagcgtcgg ggggcccgtt gcttcgcaca atggaccggc agatgccgaa gtcaatcgga
      781 cgaattcccg atcgcttgcc caccacctcg ctcccgccgc ggaccagctc catgccccag
      841 ggtttgtcgg cctcctgggg ctcttgccat aagtacgggc accggcgttg ccgcccgtca
      901 acggacgtta gtgggatgaa gtgcccggca ctcccccaga ccgcaatctg aacaggacct
      961 gcgggggttc acaggtactg gggcgggtcc tatggtgttt cacaggcagc ccgccagcaa
     1021 acccaggcca tcagccattc gtactgggtt cgcatgggca gccgacggga gttgtatcgc
     1081 gcatcgcatc ggcacagcca acaccaggtt cgccgtcatt ctgacggtgg ccaccctcct
     1141 cgggctcgcc ttgacccata tcctcaccgt cgcgccaaca gggattgcgg ccgccgaggt
     1201 tccatcaccc gcggccccgc aggctccctc aatcacgccg atgccgtcct ccgtcccaca
     1261 gctccgcgtc ccggtcgtcg cgatcgaccc cgtcatcccg gcggacgagc tggtcgcgag
     1321 gccatcggcg aacgagcgag aggccgcgac cgctgcgcgt gcaaccgcca cggctgctcc
     1381 gagcgcggca ccgagccgcc tcgtggccct ggcgcccctg acgatcgacg tgtggaatgc
     1441 cgacgagaga tacttcagcg tcagcggatc gacccccgac gagatcgtgg cctcggcggt
     1501 ggccaacgtt ccgcccgacc cctccggcgc cgctcgcagc acgatggcct acgtgggacc
     1561 ggtcacctgg aatcatcgcc cgtcatacgt gcaggacccg gcaaccggca gctgcaccat
     1621 gactgcggtg gcaagcaccg tcgcgtacca ggcgaccctg ccgcagtgga cttcgccgtc
     1681 gagcgttcag ccggagctgg tggcgtggtg gcacctcgtc ctcgatcacg tccgggcaca
     1741 cgagagcgag cacgtccgga tctacgaggg cttcgtgatc accctcccgg atcgtgtcgt
     1801 cggtcagccg tgcgcggaat gggacgggat catcggtaag tggatcgccg atctcaccgt
     1861 cgcgcaatcc gcgtttgacg ccgccgagtc ccactggtcg ctgccggtgt acacgccggg
     1921 cgagtagcca tcggccgccg gcggtccgaa ggacctgctg gctcccggat cgaaacgtcg
     1981 caatgattct ggggagcgct acgcggcggc tcggtgggct gtctccccca gctgagcgtg
     2041 gccgtgctca tgtgatggac attccgaccg atcagcggtc atggaaggcg atggtgtcca
     2101 cctggcgggc gttacgacac ctcgcagcca tgccgcggcg caagcgacca caaggccatc
     2161 gtggcggcgc tgagccgcga cgcgcgcccc cagctactcg atctcgatct tctcgtgggg
     2221 gcgggccgcg gtggccgcct gctcttcctc gtcgaggcgc tcgatccact tgtcgaggta
     2281 gacccgggcg gccatgagct gctcgcgccc ggccgtcttg aggtgcttgc gcgcctcctc
     2341 ggggaagagc cgatgcatca gcgcccagaa ggcctcctcc atgccggagc gcccctcgtc
     2401 gacggtctgt cgccagctgc ggcgctcgag ctcggcgatg cgtgcctcga gctcggcact
     2461 cgggtcgcga tcggcggggg acggtggctg gt
//
LOCUS       DATACA010000396         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2782506, whole genome shotgun sequence.
ACCESSION   DATACA010000396 DATACA010000000
VERSION     DATACA010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2782506"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1612)
                     /locus_tag="VHK28_08150"
     CDS             complement(158..1612)
                     /locus_tag="VHK28_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEX2142224.1"
                     /translation="MATRAAAAFRRSAIVAAVLSFVLPGLGHGWLGSFRRGLVLAMPV
                     LVLVGFAIGTVAFQGKIRTLGLLVQPGVLVTLLVVNLALLIWRAAAIVDAYLLARRWS
                     EPRSQSSDAAQRAIAMVVLSALLVTTLGMHAGLGFIGLKAYDTVTTVFATQEPTATPS
                     PSTAPPASQLPGEATPGPTSSPTQVPRWNDDGRLDVLLVGGDSGPGRWSLRTDTMILL
                     SVDVASGRAAMFGIPRNMRNVPLPPGPAAAFPECDCFPDLLNSLYVFAGEHPELFRGG
                     EARGYLALQDAVGQLVGRELDGTLVVTLNGFVRLVDALGGLEITTPYSLYDATYPHED
                     GRHKEVIWIPAGHHHLDGHLALAFARSRHQDTDYDRMYRQQLVVASLRRQMCPGDLVM
                     RIPELLDIARDSLWTNVAIGDVPNLLALGNRVSSDSIGRYQFWPPEIPQDLTDGGIAR
                     IRQMVAGAFPPTAAAPGVSGSATASPSPAPADSC"
     gene            1677..2060
                     /locus_tag="VHK28_08155"
     CDS             1677..2060
                     /locus_tag="VHK28_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HEX2142225.1"
                     /translation="MIVRSLDEVVGTDRDVAGEGWRSRRLLLRRDGMGFSLHDTTVAA
                     GAELELEYRHHLEACYCLEGEAEVEDLATGERHVIGPGTVYALDRNDRHVVRVSRDLR
                     LVCVFNPALSGTETHDESGSFPPSE"
BASE COUNT          350 a          764 c          815 g          318 t
ORIGIN      
        1 gccccactcg gcctgtgcgg ctgtgctgca caaccggggt ctgacagggc cgccggtggc
       61 tcactggccc gccaggacct accggcgcgc gctgtcaggg tcgattgccc cgtgcggctg
      121 tgcgcctgtg ctgcacgacg cgcccccgcc actcccctca gcagctgtcg gcaggcgccg
      181 gagaagggct ggccgtcgcc gagccggata cccccggtgc ggcggctgtc ggcgggaagg
      241 cgccggcgac catctggcgg atgcgggcga tgccaccatc ggtcaggtcc tgcggaatct
      301 cgggcggcca gaactggtag cggccgatgc tgtcggacga cacccggttg cccagcgcca
      361 gcaggttcgg cacgtcaccg atcgccacgt tggtccacag cgagtcacgc gcgatgtcga
      421 gcagctccgg gatgcgcatc accaggtcac cggggcacat ctggcgacgg agcgaggcga
      481 ccaccagctg ctgacggtac atgcggtcgt agtcggtgtc ctggtggcgc gagcgggcga
      541 aggccagggc caggtgccca tccaggtggt ggtggccggc cgggatccag atgacctcct
      601 tgtgacgacc gtcctcgtgg gggtaggtgg cgtcgtacag ggagtagggc gtggtgatct
      661 ccaggccgcc cagcgcatcc accagccgca cgaatccgtt gagcgtcacc accagcgtgc
      721 cgtccagctc tcggccgacc agctggccaa ccgcgtcctg cagcgcgagg tagccccgtg
      781 cctcccctcc gcggaacagc tcgggatgct cgccggcgaa gacgtacagc gagttcagca
      841 ggtccgggaa gcagtcacac tccgggaagg cggccgccgg cccgggtggc agcggcacgt
      901 tgcgcatgtt gcgcgggatg ccgaacatgg ccgcccgccc ggaggccacg tccacgctga
      961 gcaggatcat ggtgtcggtg cgcaggctcc agcggccggg tcccgagtca cctcccacca
     1021 gcagcacgtc caaccgcccg tcgtcgttcc agcgcggcac ctgcgtgggc gaggacgttg
     1081 gccccggcgt ggcctcgccc ggcagctgcg aagccggcgg ggcggtggat ggggacggcg
     1141 tggcggtcgg ctcctgggtg gcgaatacgg tggtcaccgt gtcgtacgcc ttcagaccga
     1201 tgaaccccag cccggcgtgc atccccaggg tggtgaccag cagcgcggac agcaccacca
     1261 tggcgatcgc ccgctgcgcg gcgtcgctcg attggcttcg cggctccgac cagcggcgag
     1321 ccagcaggta ggcgtccacg atggccgcag cgcgccagat cagcagcgcg aggttgacca
     1381 ccagcagcgt gacaaggacg ccgggctgca ccagcaggcc cagggtccgg atctttccct
     1441 ggaaggcgac cgtgccgatg gcgaagccca ccaacaccag gaccggcatg gccagcacca
     1501 gcccgcggcg gaacgatccg agccagccat gtcccaggcc ggggagcacg aaggagagca
     1561 cggccgccac aatggcggat cggcggaacg ccgcggctgc tcgggtagcc acgatccgag
     1621 tctagccttg cccgcccggc gagcactggg caattcggac catgaaggag gcctggatga
     1681 tcgttcgttc cctggacgag gtggtgggta ccgatcggga cgtcgccggc gagggatggc
     1741 gcagccgaag gctcctccta cgacgcgacg ggatgggctt ctcgttgcat gacaccaccg
     1801 tggccgccgg cgcggagctg gagctcgagt accggcacca cctcgaggcc tgctactgcc
     1861 tcgagggaga ggccgaggtc gaggacctgg ccaccgggga gcgccacgtc atcggtccag
     1921 ggacggtgta cgcgctggac cgcaacgacc ggcacgtggt gcgggtctcg cgcgacctgc
     1981 ggctggtgtg cgtcttcaac cccgcgctga gcggcaccga aacccacgac gagtccggga
     2041 gcttcccgcc gtccgagtga gggccagctg ctcctttccg aattcacgtg agatgcgcac
     2101 ccgtggcgcg tcaggggcct tgaacgggct ggattcacac gcggtgtcaa tggaggcgct
     2161 tcgcggcccg ttttcagcgc gtggtggtca ggtggtgtga atccggacgc ctcactcgcc
     2221 ggtcggagtg gccgtgatgt gcagtcc
//
LOCUS       DATACA010000397         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1418054, whole genome shotgun sequence.
ACCESSION   DATACA010000397 DATACA010000000
VERSION     DATACA010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418054"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..477)
                     /locus_tag="VHK28_08160"
     CDS             complement(34..477)
                     /locus_tag="VHK28_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX2142226.1"
                     /translation="MIAAIRKRLEQRDEGFTLIELLVVVIIIGILAAIAIPTFLSQRE
                     RGWQAELTSAVRNAALEIEAAATQTGGTYPATAAGQTLVNDFLQEGLGQTSTTDPVGL
                     TYAFVPADATAGTPAGFTIRGVHTTLPGATQDYNSSAGGLQDFQG"
     gene            complement(669..1898)
                     /locus_tag="VHK28_08165"
     CDS             complement(669..1898)
                     /locus_tag="VHK28_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX2142227.1"
                     /translation="MATTAVKFDYQVRDRAGKTINGHLEGPNQQAVAAKLREMGYAPV
                     SITVAKGSALQKEISIPGFAKKVKTEDLAVFSRQFATMINSGVSLIRSLNILAEQTEN
                     EKLADIIKVVRSDVEEGQSLSDAVAKHPDTFPKLYVAMVRAGETAGMLDSVLLRVADT
                     MESDVALKRKIKSALTYPVVVLIMALVLCGVMLVFIVPTFVGLFESLGGELPLPTKVM
                     LALSSLLRSFWYVIMFLPVLALKAFQQARKNPDVRFQLDRLKLKLPVFGNLFHKIALS
                     RFARNLGTLLRAGVPILTALEITADTVNNGVIGNATNDVKTSVKEGESVARPLAQHEV
                     FPPMVVQMLAVGEETGAMDEMLVKISDFYDGEVTATTESLTAMLEPLMIGVLGGIVGA
                     MVIALYMPMFKIFDLIQ"
     gene            complement(1927..3141)
                     /locus_tag="VHK28_08170"
     CDS             complement(1927..3141)
                     /locus_tag="VHK28_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX2142228.1"
                     /translation="MADDSPAPAPAAAAAPASASAAPAAVSPATAPQPAKVSASGPLL
                     AFEEDSEFDLNALLTELKNRGGSDLHLTAGFPPAIRIHGELLAVEGQPVLTPPELQKM
                     LYSILTQKQRERFENTLELDLSYQVPGEARYRVNIFQQRESLGAVMRIIPFEIVPIEK
                     LGVPAQVTNFAHLPRGFVLITGPTGSGKSTTLASLIDVINTERAAHIMTVEDPIEFLH
                     RHKRSVVNQREVGTDTHGFTQALKHVLRQDPDVILVGEMRDLETIQIALTAAETGHLV
                     FGTLHTQDAPQTVDRVVDVFPPEQQEQIRVMLSGALQGVVCQQLLKTADGTGRVVATE
                     VMVATSAIKNLIREGKTHQMYSMIQAGKQHGMVTMDQSLADLVLKGRITYNAALERCN
                     NVGDFNRLCGRA"
BASE COUNT          488 a          969 c         1134 g          558 t
ORIGIN      
        1 gcccgtaggc cgccccttcc aaacgctgtg ctgctaaccc tggaaatcct ggagaccacc
       61 agcgctgctg ttgtagtcct gagttgcgcc agggagggtc gtgtgaacgc cccggatcgt
      121 aaagccagcc ggagtaccgg cggtcgcgtc agcgggaacg aaggcgtagg tcaggccgac
      181 cgggtcggtg gtgctggtct ggcccaggcc ctcctgaagg aagtcgttga ccagggtctg
      241 acccgcggcg gttgcgggat aggtgcctcc ggtctgggtg gcggcagcct cgatctcgag
      301 ggcggcgttg cggacggcgc tggtcagctc ggcctgccag ccgcgctcgc gctgcgacag
      361 gaaggtcggg atggcgatgg cggcgaggat gccgatgatg atgacgacga cgaggagctc
      421 gatgagggtg aagccctcgt cacgctgctc caggcgcttg cggatcgctg cgatcatgtg
      481 atgcgctcct tgatggagag gagaatgtgt gcgatgacct cttctctctc ggccagcgcc
      541 tggctcttct tgagcagcta cctgtgaaag ttctgcgcac gcaagaggcc ggtgtcgatt
      601 ggggcgccta tagctaccca acgtgacacc ggcctctgcg ctgcggcgct gtcgcgccgg
      661 cggttcgcct actggatcag gtcgaagatc ttgaacatgg gcatgtacag ggcgatgacc
      721 atcgcgccga cgatgccgcc caggacgccg atcatgagcg gctcgagcat ggcggtgagg
      781 gactcggtgg tcgcggtcac ctcgccgtcg tagaagtcag agatcttgac cagcatctcg
      841 tccatggcac cggtctcctc gcccacggcg agcatctgca cgaccatggg cgggaagact
      901 tcgtgctggg cgagcgggcg cgcgacgctc tcgccctcct tgaccgaggt cttgacgtcg
      961 ttggtcgcgt tgccgatgac gccgttgttg acggtgtcgg ccgtgatctc cagcgccgtc
     1021 aggatcggca cgccggcacg cagcagcgtg cccaggttcc gcgcgaagcg cgacagcgcg
     1081 atcttgtgga acaggttccc gaacacgggc agcttgagct tgaggcgatc gagctggaag
     1141 cgaacgtctg ggttcttgcg cgcctgctgg aaggccttga gggcgagaac cggcaggaac
     1201 atgatcacgt accagaagct gcgcagcagg ctcgagaggg ccagcatcac cttggtcggc
     1261 agcggcagct ccccgccgag tgattcgaac aggcccacga acgtcggcac gatgaacacc
     1321 agcatcacgc cgcacagcac gagcgccatg atcagcacga cgacggggta ggtcagcgcg
     1381 gacttgatct tgcgcttcag cgctacgtcg gactccatcg tgtcggcgac gcggagcagg
     1441 acggagtcga gcatgcccgc cgtctctccg gcgcggacca tcgcgacgta cagcttcggg
     1501 aacgtgtcgg ggtgcttggc gaccgcgtcg gacagggact ggccctcctc gacgtcgctg
     1561 cggaccacct tgatgatgtc ggcgagcttc tcgttctccg tctgttcggc gaggatgttg
     1621 agcgaccgga tcagtgacac gcccgagttg atcatcgtgg cgaactggcg cgagaacacg
     1681 gccaggtcct cggtcttcac cttcttggcg aagcccggga tggagatctc cttctggagc
     1741 gcgctcccct tggccactgt gatggagacc ggtgcgtagc ccatctcccg cagcttggcg
     1801 gcgaccgcct gctggttcgg cccttcgagg tggccgttga tggtcttgcc ggcccggtcg
     1861 cggacctggt agtcgaactt gacggcggtg gtcgccacgg tgctgctcct tagtgtcccg
     1921 gcgagctcag gcccggccgc agaggcggtt gaagtcgccg acgttgttgc agcgctcgag
     1981 cgccgcgttg taggtgatcc ggcccttgag gacgaggtcg gccagggact ggtccatggt
     2041 caccatgccg tgctgcttgc cggcctggat catcgagtac atctggtgcg tcttgccctc
     2101 gcggatgagg ttcttgatgg ccgacgtggc gaccatcacc tcggtcgcga cgacgcgacc
     2161 ggtgccgtcg gcggtcttga gcagctgctg gcagacgacc ccctggaggg cgcccgagag
     2221 catcacgcgg atctgctcct gctgctccgg cgggaacacg tcgacgaccc ggtcgaccgt
     2281 ctggggcgcg tcctgggtgt ggagggttcc gaacaccagg tgcccggtct cggctgcggt
     2341 cagcgcgatc tggatggtct ccaggtcgcg catctcaccg acgaggatca cgtccgggtc
     2401 ctggcgcagg acgtgcttga gcgcctgcgt gaacccgtgc gtgtccgtgc cgacctcgcg
     2461 ctggttgacg accgagcgct tatggcggtg gaggaactcg atcgggtcct cgacggtcat
     2521 gatgtgggcg gcgcgctcgg tgttgatgac gtcgatcaac gaggcgagcg tggtcgactt
     2581 gccggagccc gtcggacccg tgatcaacac gaagccacgc ggcaggtggg cgaagttggt
     2641 cacctgggcg ggcacgccga gcttctcgat cggcacgatc tcgaacggga tgatgcgcat
     2701 gaccgcaccg agcgactccc gctgctggaa gatgttgacg cggtagcggg cctcgccggg
     2761 cacctggtag gacaggtcga gctcgagggt gttctcgaag cgctcgcgct gcttctgcgt
     2821 caggatgctg tacagcatct tctgcagctc aggcggggtc agcaccggct ggccctcgac
     2881 cgccagcagc tcgccgtgga tccggatggc gggcgggaac ccggcggtca ggtgcaggtc
     2941 ggaaccgccg cggttcttga gctcggtcag cagcgcgttg aggtcgaact ccgagtcctc
     3001 ctcgaacgcg agcagcgggc cggacgccga caccttggcg ggctgggggg cggtggcagg
     3061 agacacagcg gccggcgccg cggaggcgga ggcgggggca gccgctgcag ccggggccgg
     3121 ggctgggctg tcgtcggcca ggtgctcga
//
LOCUS       DATACA010000398         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_855747, whole genome shotgun sequence.
ACCESSION   DATACA010000398 DATACA010000000
VERSION     DATACA010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855747"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..827
                     /locus_tag="VHK28_08175"
     CDS             69..827
                     /locus_tag="VHK28_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142229.1"
                     /translation="MRRAAAGVLSLTAFLVAWKLVTILSGTPEYILPAPEVVWERALR
                     AMGIGPGGTGILWEHLRATVEEVMLGFALGAAAAIVTGVALGKSIVVERLLSPYIVAA
                     QAVPILALAPLLDIWFGGGLLARVLVCALIVFFPIAIATMVGIRSADPLLREMLRSLG
                     ATPAQLTTRLEIPSALPVIFGGLRVGVTLSVIGAVVAEWVSASRGLGVLINIADQGLF
                     DTPLMFVALAALAIIGLVFYYLVALVERRLVRHV"
     gene            836..1879
                     /locus_tag="VHK28_08180"
     CDS             836..1879
                     /locus_tag="VHK28_08180"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2142230.1"
                     /translation="MPIHRSIPSRLAPGVVMVVALVLNLATCSSPSPTPVPSTSAEPS
                     REPLREVRLLLGFRPDVQFAPFYLAQQAGHFADEGLQVSIEHSNDTIRLVADGQAQFG
                     VADATDIMIGRTAGIPIRYVSTLYERFPVALIGARGALPADPRGLAGRTIGTPGRFGS
                     SWHALLALLDAGGLTADDVTIREYPQFNQAEGLLNGDVDLITGFRNNEPIQLAQRGYQ
                     AGLITLDQVAPLPGPGVVVGDELLAAEPQLVEAFTRAVASAQAAVIDDPELGLAAARE
                     AVPAIGEDPETAAAVLDATAALWAGSGGFAEGRIDIERWRDAYQLMQRLGFIDGSVPV
                     EEMIAPEVRGEAP"
     gene            complement(1910..2821)
                     /locus_tag="VHK28_08185"
     CDS             complement(1910..2821)
                     /locus_tag="VHK28_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEX2142231.1"
                     /translation="MSTRILDGRTVASRLWRELTDRVAALREGGADEPRLAVVRLGDD
                     GPSAVYAASLGRAARSVGIEPVEVPAPGLGRADLAARIGALNREPGISGIVVAQPLPA
                     GLDAPAVLDMVDPAKDVDGATALNAGRLARGEAAFAPATALSVMAILRTHQVPIAGRR
                     AVVVGRSAVVGRPTAALLTAADATVMVCHRRTRNLARETRRAEILVVAAGQPGLIRSD
                     MVNRSAVVIDCGINTTPEGIVGDVDFAAVRPVVAAITPVPGGVGPVTTMMVLRQTVEA
                     AERAAAERDPLSDRYTELPAAPGAARP"
     gene            complement(2818..>3314)
                     /locus_tag="VHK28_08190"
     CDS             complement(2818..>3314)
                     /locus_tag="VHK28_08190"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX2142232.1"
                     /translation="RPDAADHEDQLLEARSVATSHLAALLDLADEDAAAYHSVVEARR
                     LPRSSDQEQAARLLALNSAMQRAAEAPLRTAQTAARVLDLANAVAPIGNLNAVSDAAV
                     AALLAAASVRGAALNVRINLPYLPAGDPLVAIAPEELARLEQLAGELERSALDIVARR
                     MARP"
BASE COUNT          413 a         1199 c         1212 g          490 t
ORIGIN      
        1 gccgttcgcc gcgcgttgag cgacgtgcac ccgcccgagc tgcggccgtg gacccagggg
       61 ggcagcgcat gagacgggcc gcggccggag tgctctcgct gacggccttc ctggtggcat
      121 ggaagctcgt caccatcctc agcggcacgc ccgagtacat cctgccggcc ccggaagtgg
      181 tctgggaacg ggcgttgcgg gccatgggca tcggtcccgg aggcacgggc atcctgtggg
      241 agcacctgcg ggccaccgtg gaggaggtga tgctgggctt cgcgctcgga gcggcggcgg
      301 ccatcgtgac cggcgtggcg ctcggcaaga gcatcgtggt cgagcgcctg ctgtcaccct
      361 acatcgtggc tgcccaggcg gtgccaatcc tggccctggc gccactgctc gacatctggt
      421 tcggcggcgg cctgctggcg agggtccttg tctgcgccct gatcgtcttc ttcccgatcg
      481 ccatcgccac catggtcggc attcgctcgg cggaccccct gctgcgcgag atgctgcgct
      541 cgctcggggc caccccggcg cagctcacca cccgcctgga aattccctcc gcgctgccgg
      601 tcatcttcgg cggcctgcgg gtgggcgtca ccctgtcggt gatcggggcg gtcgtggcgg
      661 agtgggtcag cgccagccgc gggctcgggg tgctgatcaa catcgccgac cagggcctgt
      721 tcgatacgcc gctgatgttc gtcgcgctcg ccgcgctggc catcatcggc ctggtcttct
      781 actaccttgt ggcactcgtc gagcgtcggc tcgtccggca cgtttgaggt cttgcatgcc
      841 aatccatcgg tccattcctt cgcgcctcgc gcccggcgtg gtcatggtcg tggccctggt
      901 gctcaacctg gcgacctgtt ccagtccctc acccaccccg gtcccctcga cctcggctga
      961 accctcccgc gagccgctgc gcgaggttcg cctgctgctc ggcttccggc cggacgtgca
     1021 gttcgcgccg ttctacctcg cgcagcaggc cggccacttc gccgacgagg gcctgcaggt
     1081 cagcatcgag cacagcaacg acaccatccg gctggtggcg gatggtcagg cacagttcgg
     1141 cgtggccgac gcgaccgaca tcatgatcgg gcgcacggcg ggcatcccga tccgctacgt
     1201 ctcgaccctg tacgagcggt ttccggtggc gctcatcggg gcgcgaggcg ccctgcccgc
     1261 cgatccgagg gggcttgcgg gccggaccat cgggacgccc ggccgattcg gttcgtcctg
     1321 gcacgcactg ctggccctgc tggatgccgg cggtctgacg gcggatgacg tgaccatccg
     1381 cgagtacccc cagttcaacc aggcggaggg cctgctgaat ggggacgtcg atttgatcac
     1441 cggcttccgc aacaacgagc cgatccagct ggcgcagcgc ggctaccagg caggcttgat
     1501 caccctcgac caggtcgcgc cgctgcccgg gccgggggtc gtggtcggcg atgagctgct
     1561 ggcggccgag ccgcagctcg tggaagcctt cacgcgcgcg gtggccagcg cgcaggcggc
     1621 ggtgatcgac gacccggagc tggggctggc ggcggcgcgc gaggccgtcc cggccatcgg
     1681 cgaggatccc gagacggccg ccgcggtgct ggacgccacc gcggccctgt gggccggcag
     1741 cggcgggttc gccgagggcc gaatcgatat cgagcgctgg cgggatgcct accagctgat
     1801 gcagcgactc gggttcatcg acggctccgt ccccgtggag gagatgatcg cgccagaggt
     1861 gcgcggagag gcgccctagc ccgcgggcgt cccttgggcc aggcggcgct cagggccgcg
     1921 cggcgccggg cgcggcaggc agctccgtgt agcggtcgct gagcgggtcg cgctccgcgg
     1981 cggcgcgctc ggccgcctcc accgtctggc gcaggaccat catggtggtc accgggccga
     2041 ccccgccggg caccggcgtg atggcggcca ccaccgggcg caccgcggcg aagtccacgt
     2101 cgcccacgat gccctccggc gtggtgttga tgccgcagtc gatcacgacc gccgagcggt
     2161 tgaccatgtc ggagcggatc agtcccggct gtccggccgc caccaccagg atctcggcgc
     2221 gccgcgtctc gcgcgccagg tttcgcgtgc ggcgatggca gaccatgacc gtggcgtcgg
     2281 cagcagtgag cagggctgcg gtaggtcgtc cgaccacggc cgagcgaccg accaccactg
     2341 cccgcctgcc ggcgatgggc acctggtggg tccgcaggat ggccatcacc gacagcgcgg
     2401 tggcgggcgc gaaggccgcc tcgccgcggg ccaggcgtcc ggcgttgagg gcggttgcgc
     2461 cgtcgacgtc cttcgccggg tccaccatgt ccaggaccgc tggagcgtcc agccccgcgg
     2521 ggagcggctg ggcgacgacg atgccggaga tgcccggctc acggttgagg gcgccgatgc
     2581 gcgccgcaag gtcggcacgg cccaggccgg gagccggcac ctccacgggc tcgatgccca
     2641 cgctgcgcgc cgcgcggccc aggctggctg cgtacacggc cgacgggccg tcgtcgccga
     2701 ggcgcacgac ggccagtcga ggttcgtctg ccccgccctc gcgcagcgcg gcgacacgat
     2761 cggtcagctc tcgccagagt cgcgaagcga cggtgcgccc atccaggatg cgcgtgctca
     2821 tggccgcgcc atccttcggg cgacgatatc cagcgcggag cgctcgagtt cgcccgcgag
     2881 ctgctccagg cgagcgagct cctcgggggc gatggcgacc agggggtcgc cggcgggcag
     2941 atacggaagg ttgatgcgca cgttgagcgc cgccccccgc accgaggccg ccgccagcag
     3001 ggcggcgacc gccgcgtcac tcaccgcgtt caggttgccg atgggcgcga ccgcgttcgc
     3061 caggtcgagg actcgcgccg cggtctgcgc cgttcggagc ggggcctcgg ccgcgcgctg
     3121 catggccgag ttcagcgcca gcagccgagc cgcctgctcc tggtcactgc tgcgcggcag
     3181 ccggcgcgcc tcaaccaccg agtgataggc ggccgcgtcc tcatcggcca ggtccagcaa
     3241 cgccgccagg tggctggtcg caaccgacct ggcctccagc agctggtcct cgtggtcggc
     3301 cgcgtccggc cggc
//
LOCUS       DATACA010000399         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_614696, whole genome shotgun sequence.
ACCESSION   DATACA010000399 DATACA010000000
VERSION     DATACA010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614696"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1142
                     /locus_tag="VHK28_08195"
     CDS             135..1142
                     /locus_tag="VHK28_08195"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX2142233.1"
                     /translation="MSGAIAVVGAGAWGTTLAINLARAGHPVTLWAHSAAAAGELESD
                     RENRRYLPGVPFPADLRVSADDQALGEAHRSYILAVPSAHVRNTLGRLSEALDPSTPV
                     LSVVKGIEEQSRLRMSQVIGEVLPGRRVAVLSGPNLAREIAAGKPAGSVVACEDGSLA
                     AELASMLGTDRFRVYTNPDVVGVELCGALKNVVALAAGMVDGLGMGDSAKAGIITRGL
                     AEMTRLGVAAGANPMTFAGLAGVGDTIATCMSHLSRNRRAGELMASGLAWTDASAQLN
                     GVAEGVDTVRGALALAAAHGVELPIAEQVAAVVHEGRSPMEAVAALMSRSQKDELAGS
                     P"
     gene            1197..>2918
                     /locus_tag="VHK28_08200"
     CDS             1197..>2918
                     /locus_tag="VHK28_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2142234.1"
                     /translation="MSEARATPAVEPGGLDRAGEVLGHAAGPLRPFFAPRSVAVIGAT
                     ESRGKVGRTLMANLISNAFGGTVFPVNARRPSVLGVKAYPTIGDVPDPVELAVVVTPA
                     PTVPGVIEECVAAGVRAAIVISAGFKEVGPEGVALEQRILATARESGMRIIGPNCLGV
                     MNPIQGLNATFAAGIARPGSVGFVSQSGALLTAILDWAEHEQTGFSAIVSLGSMLDVG
                     WGDVIYHLGDDSNTGSIVIYMETIGDARAFLSAAREVALTKPIIVIKPGRTEAAARAA
                     ASHTGSLTGSDEVLDTAFRRAGVLRVEAIDDLFGMAEVLAKQPRPRGRRLSIVTNAGG
                     PAVLATDELISGGGELAPLSDETLTQLSGFLPDAWSHNNPIDVLGDADAERYRQAVEV
                     ASANPDSDGLLVILTPQDMTEPTETARALLPYAHQQNKPVLGSWMGGRMVADGVAQLQ
                     SAGIPSFAYPDAAVRMFNYLWRFADNLRLLYETPVLPGDMDGHASRTAVRAVVEGAAR
                     DGRTLLTEVESKQVLAAYGIPVTETRVAATEDEAVAAAEAIDYPVVAKLYSRSITHKT
                     DVGGVKLN"
BASE COUNT          424 a         1016 c         1085 g          393 t
ORIGIN      
        1 gagacgcgcc gccacatgaa ggcggccggg cgagagcagc tgctcgcggc ccgcagctac
       61 ctggaccact ggatcgccaa gatggacgag cccgccgccc gcgagccgga gcagcgcgag
      121 acgatcaccg tcgagtgagc ggggcgatcg ccgtcgtcgg cgccggcgcg tgggggacga
      181 cgctcgccat caacctggca cgcgccggcc accccgtgac cctctgggca cacagcgccg
      241 ctgccgctgg cgagctcgag tcggaccggg agaaccggcg ctaccttccc ggcgtcccgt
      301 tcccggccga cctgcgggtc agcgccgacg accaggcact cggcgaggcc catcggtcgt
      361 acatcctggc cgtcccgtcg gctcacgtgc ggaacacgct cgggcggctg agcgaggcgc
      421 tggatccgtc caccccggtg ctgtcggtgg tgaagggaat cgaggagcag agccggctgc
      481 gcatgagcca ggtcatcggc gaggtactgc ccgggcggcg ggtggcggtc ctctcggggc
      541 cgaacctggc gcgcgagatc gcggccggca agccggccgg gagcgtggtg gcctgcgagg
      601 acggcagcct ggcggccgag ctggcgtcga tgctggggac cgaccgcttc agggtctaca
      661 ccaatcccga cgtggtgggc gtcgagctgt gcggcgcgct gaagaacgtg gtcgcgctgg
      721 cggccggcat ggtcgacggc cttgggatgg gcgacagcgc gaaggccggc atcatcaccc
      781 gcggcctggc cgagatgacc cgcctgggcg tggcggccgg cgcgaaccca atgaccttcg
      841 ccgggctcgc cggggttggt gacacgatcg cgacctgcat gagccacctg tcgcgcaacc
      901 ggcgtgcggg cgagctgatg gcctccggcc tggcctggac cgatgcctcc gcgcagctca
      961 acggcgtggc ggagggtgtc gacaccgtcc gcggggcgtt ggccctcgcc gcggcacacg
     1021 gggtggagct gccgatcgcc gagcaggtgg ccgcggtggt gcacgagggc cggtcgccga
     1081 tggaggcggt ggccgccctg atgtcccgct cccagaagga cgagctggcc ggctccccct
     1141 gacgcggcgg gcccaggtgg ccggcatcct gctgctcacc cggtagagtg gtgaggatgt
     1201 ccgaggcgcg agcgacaccc gcggtcgagc cgggcggcct ggaccgcgcc ggcgaggtgc
     1261 tgggacacgc ggccggcccg ctgcggccct tcttcgcgcc gcgctcggtg gcggtcatcg
     1321 gggccaccga gtcgcgtggc aaagttggcc ggacgctgat ggccaacctg atcagcaacg
     1381 ccttcggcgg caccgtcttc ccggtcaatg cgcgacgccc gagcgtgctg ggcgtcaagg
     1441 cctacccgac catcggcgac gtcccggacc cggtcgagct ggcggtggtg gtgacgcccg
     1501 ccccgacggt cccgggagtg atcgaggagt gcgtcgctgc cggggtgagg gccgccatcg
     1561 tgatcagcgc cggcttcaag gaggtaggcc cggagggcgt cgcgctggag caacggatcc
     1621 tggccaccgc gcgcgagagc ggcatgcgga tcatcggtcc caactgcctc ggcgtcatga
     1681 acccgatcca gggcctgaat gccacctttg ccgcggggat cgcccggccg ggctcggtcg
     1741 gcttcgtgag ccagagcggc gcgttactga ccgccatcct cgactgggcc gagcacgaac
     1801 agaccgggtt ctcggccatc gtctcgctgg gctccatgct cgacgtcggc tggggcgacg
     1861 tcatctacca cctgggggac gactccaata caggaagcat cgtcatctac atggagacga
     1921 tcggcgacgc gagggcattc ctgtccgccg cccgcgaggt ggcgcttacc aagccgatca
     1981 tcgtcatcaa gcccggtcgc accgaggccg cggcacgggc ggccgcctcg cacaccggct
     2041 cgctgactgg ctctgacgag gtgctcgaca ccgcgttccg gcgggctggc gtcctgcgcg
     2101 tggaggccat cgacgacctg ttcggcatgg ccgaggtgct cgccaagcag ccgcgcccgc
     2161 gcggccgccg gttgagcatc gtgaccaatg ccggcgggcc ggcggtgctg gccaccgacg
     2221 agctgatctc gggtggcggc gagctggcgc ccctcagcga cgagacgctg acccagctca
     2281 gcggctttct gccggacgcg tggagccaca acaacccgat cgacgtgctg ggcgacgcgg
     2341 atgccgagcg ctaccgccag gcggtggagg tggcgtccgc caacccggac tcggacgggc
     2401 tgctggtcat cctcaccccg caggacatga ccgagccgac ggagacggcg cgagccctgc
     2461 tgccctacgc ccatcagcag aacaagccgg tcctgggcag ctggatgggc gggcgcatgg
     2521 tggccgacgg cgtggcacag ctccagtcgg ccgggatccc ctccttcgcc tacccggacg
     2581 cggcggtccg catgttcaac tacctgtggc gcttcgccga caacctgcgc ctgctgtacg
     2641 agacaccggt gctgcccggt gacatggacg gacacgccag ccggacggcg gttcgggccg
     2701 tggtcgaggg cgctgcacgc gacggccgca cgctgttgac cgaggtcgag tccaagcagg
     2761 tgctggccgc ctacggcatc ccggtgaccg agacccgggt ggcggccacc gaggatgagg
     2821 cggtggcggc cgccgaggcc atcgattacc cggtggtcgc caagctgtac tcgcgcagca
     2881 tcacccacaa gaccgatgtc ggcggggtca agctcaac
//
LOCUS       DATACA010000400         4623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2140522, whole genome shotgun sequence.
ACCESSION   DATACA010000400 DATACA010000000
VERSION     DATACA010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140522"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VHK28_08205"
     CDS             complement(<1..442)
                     /locus_tag="VHK28_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2142235.1"
                     /translation="MSRLRELRLHGFKTFADPTRFVFEPGVNAVIGPNGSGKSNMADA
                     VRWVLGEQSNRSLRTRRADDVIFVGSQHRRPQGMAEAVLVLDNEDGWLPIDFAEVSVG
                     RRAYRSGESEYLINGARARLRDVVELLGAGRLGANELVVVGQGTV"
     gene            complement(439..1149)
                     /gene="rnc"
                     /locus_tag="VHK28_08210"
     CDS             complement(439..1149)
                     /gene="rnc"
                     /locus_tag="VHK28_08210"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX2142236.1"
                     /translation="MATPSLDDLGDRLGIHLRDPGAIRQAFVHSSYHNENPQLVAGHN
                     ERLEFLGDAVLGLIVSRLLYERFPDEDEGFLTARRAALVNRDALASLALDLGLDRYLL
                     LGRGEAEAGGARRPSLLAATVEALAGAVYLAEGLEATRDWLTRLFEDLVQPDGADGPL
                     KSAKSRLQEWTQRHRHTKPTYEVIETVGPAHDQTFTVAAVVDGRRLGTGVGSSRQRAE
                     EQAAEEALATLGTDGSPA"
     gene            complement(1163..1405)
                     /gene="acpP"
                     /locus_tag="VHK28_08215"
     CDS             complement(1163..1405)
                     /gene="acpP"
                     /locus_tag="VHK28_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX2142237.1"
                     /translation="MTDGTTFERLKKIIVEQLGVDEEEVTPQASFVEDLNADSLDLVE
                     LIMSLEEEFGMEISDEDAEKIQKVQDAVEYIEEHQQ"
     gene            complement(1409..1876)
                     /gene="nusB"
                     /locus_tag="VHK28_08220"
     CDS             complement(1409..1876)
                     /gene="nusB"
                     /locus_tag="VHK28_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX2142238.1"
                     /translation="MAPRRRAAGHRLPSGQGFQARRLAMLALFEEEFRPEQAAAALER
                     LAEEKRARSDVLEHARQIVDGVLGRRAELDAEITRHAPAIPVSELGRVERTILRSALY
                     EVLYSAATPSGETIHDAVALARTYAGDAARRLVNGVLGSVLRSSGSGASGRGA"
     gene            complement(1897..2970)
                     /gene="plsX"
                     /locus_tag="VHK28_08225"
     CDS             complement(1897..2970)
                     /gene="plsX"
                     /locus_tag="VHK28_08225"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX2142239.1"
                     /translation="MRIALDAMGGDFAPREAVRGAINYAATNADEVILVGNVPRLEAE
                     IAEYRRGRPASVTFVDAPDVIGMGESPATALRQKRRASVRVATDLVRDGLADAVVSAG
                     STGATMAAAVFRLGRVEGIDRPALGAHLVTATGPIMLLDVGANVDSDATNLVHFAVMG
                     SIYAERVLHVPRPRVGLLNIGEEPEKGTALIRETHERLAEAEVNFIGNVEGNDLIYHA
                     ADVVVTDGFTGNVVVKLFEGLTSYIFRSLRTDLQQGPVAPLALLALKPGFDRLRSRFD
                     YEKYGGVPLLGVRGVSIVTHGRARARMIENALRVASEAAAARIPELIAEWSREHPALA
                     RLDPVNGVASGTRSIAAAGPGED"
     gene            complement(2985..3188)
                     /gene="rpmF"
                     /locus_tag="VHK28_08230"
     CDS             complement(2985..3188)
                     /gene="rpmF"
                     /locus_tag="VHK28_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX2142240.1"
                     /translation="MGVPKRKVSKARQGRRRSHLAISAPPLVECTHCHELKRAHHVCP
                     TCGYYDGREVVPVESTPPEGGSR"
     gene            complement(3269..3805)
                     /locus_tag="VHK28_08235"
     CDS             complement(3269..3805)
                     /locus_tag="VHK28_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX2142241.1"
                     /translation="MTVFNVAGLLREPPGAVRGHRLRDRYVSLGPDVELAGPINGTIR
                     MQRTNRGILVRGDLEAPVRRTCARCLEPFVEPARIQLSEEYLPSLDPESGAALPEPSA
                     DEEVRAIDEHNEIDLGPVLREEFALTEPMVPLHDPNCAGLCPECGEPLAPGHPSHGEP
                     EIDPRLAALQQLLERDED"
     gene            complement(3802..4248)
                     /locus_tag="VHK28_08240"
     CDS             complement(3802..4248)
                     /locus_tag="VHK28_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX2142242.1"
                     /translation="MLEQAAVLELIDQLRVAVPDEVRQAKRITEEAGRITERAREEGD
                     AIIARAQEQAAQMLEERELVRMAQQRAGEILDAANHEAAEVRRGADEYAAGVLIRLEG
                     ECIKALTSIKRGIDMLDERHRVRPSTNDVPHDASGEPEATEAATRQ"
BASE COUNT          626 a         1673 c         1593 g          731 t
ORIGIN      
        1 ccacggtccc ctgtcccacc accaccaact cgttggcgcc cagccgcccg gcgcccagca
       61 gctccaccac gtcgcgcagc cgggcgcggg cgccgttgat caggtactcg ctctcgccgg
      121 accgatacgc ccgccgcccc acggagacct cggcgaagtc tatgggcagc cagccgtcct
      181 cgttgtccag gaccaggacc gcctcggcca tgccctgcgg gcggcggtgc tgcgagccca
      241 cgaagatgac gtcgtccgcg cgacgggtgc gaagcgatcg attagactgc tcgcccagca
      301 cccagcgcac ggcatcggcc atgttcgact tgccggatcc gttcggaccg atgacggcgt
      361 tgacgcccgg ttcgaagacg aaccgcgtgg ggtcggcgaa ggtcttgaag ccgtgcaggc
      421 gcagctcgcg aaggcggctc atgcgggcga cccgtcggta cccagcgtgg ccagtgcctc
      481 ctccgcggcc tgctcctcgg cacgctggcg cgaggaccca acgcccgtgc ccaggcgccg
      541 gccgtccacc acggcggcca ccgtgaaggt ctggtcgtgg gcggggccga cggtctcgat
      601 cacctcgtag gttggcttgg tgtgccgatg acgctgggtc cactcctgca ggcggctctt
      661 cgccgatttc agtgggccgt cggcgccatc gggctgcacg agatcctcga acaggcgggt
      721 cagccagtcg cgggtggcct ccagcccctc ggccaggtac accgcgccag ccagcgcctc
      781 gacagtggcg gccagcaggg acgggcggcg agccccaccg gcctccgcct cccctcggcc
      841 gaggagcagg tatcggtcca ggccgagatc aagcgccagg gaggcgagcg cgtcgcggtt
      901 gacgagcgcg gcgcggcgtg cggtcaggaa gccctcgtcc tcgtccggga agcgttcgta
      961 cagcaggcgg ctgacgatga gccccagcac ggcatcaccc aggaactcca gccgctcgtt
     1021 gtgcccggcg accagctgcg ggttctcatt gtggtaggag ctgtggacga acgcctggcg
     1081 aatggccccg gggtcccgaa ggtgaatgcc gagccggtca cccagatcgt cgaggctcgg
     1141 cgtggccatc agcgggcaga ggctactgct ggtgctcctc gatgtactcg acggcgtcct
     1201 ggaccttctg gatcttctcc gcgtcctcgt ccgagatctc catcccgaac tcctcctcga
     1261 ggctcatgat gagctcgacg agatcgagcg agtccgcgtt cagatcctcg acgaacgacg
     1321 cctgcggcgt gacctcttcc tcgtcaacac cgagctgctc gacgatgatc ttcttgagtc
     1381 gctcgaaggt tgttccgtcg gtcacccttt aggctcctct tcccgatgct ccactccccg
     1441 atgagcgcaa tacgctcccc agaacgccat tcacgagtcg gcgagctgcg tctccagcgt
     1501 aggtccgggc gagtgccacg gcatcgtgga tcgtctcccc gctcggggtc gcggcggagt
     1561 atagcacctc atatagcgcg ctccgcagaa tcgtacgctc cacccgaccc agctcactga
     1621 ccggtatggc gggggcgtgc cgcgtgatct cggcgtccag ctccgctcgc cggccgagca
     1681 cgccgtccac gatttggcgg gcgtgctcca gcacgtcgct gcgcgcgcgc ttctcctccg
     1741 ccagccgctc gagcgcggcc gcggcctgct ccggccggaa ctcctcctcg aacagcgcca
     1801 gcatggccag gcggcgtgcc tggaagccct gcccagacgg caggcgatgg ccggcggcgc
     1861 gacggcgagg tgccatggtc gcgcccgatg ccccgctcaa tcctcgcccg ggccggcggc
     1921 cgcgatcgac cgggtgccgc tggcgacccc gttgaccgga tcgagccggg ccagggcggg
     1981 gtgctcccgg ctccactcgg cgatcagctc cgggatgcgg gccgcggccg cctcggaggc
     2041 gacccgcagg gcgttctcga tcatccgcgc ccgcgcccgg ccgtgggtca cgatgctcac
     2101 cccgcgcacg ccgagcagcg ggacgccgcc gtacttctcg tagtcgaagc ggctgcgcag
     2161 gcggtcgaag ccgggcttga gggccagcag cgccagcggc gcgaccggtc cctgctgcag
     2221 gtccgtgcgc agcgagcgga agatgtagct ggtcaggccc tcgaagagct tgaccaccac
     2281 gttgccagtg aagccgtcgg tgaccaccac atcggcggcg tggtagatga ggtcgttccc
     2341 ctcgacgttg ccgatgaagt tcacctcggc ctccgcgagg cgctcgtgcg tctcgcgaat
     2401 cagcgcggtt cccttctccg gctcctcgcc gatgttcagc aggcccaccc ggggccgcgg
     2461 cacgtgcagc acgcgctccg cgtagatgga gcccatcacc gcgaagtgaa cgagattggt
     2521 tgcgtcggag tcgacgttgg cccccacgtc gagcagcatg atcgggccgg tggccgtcac
     2581 caggtgcgcg cccagtgccg ggcgatcgat gccctccacg cgacccagcc ggaagacggc
     2641 tgcggccatg gtggcgcccg tggagccggc gctgaccacg gcatcggcca ggccgtcgcg
     2701 aaccaggtcg gtcgccaccc ggaccgatgc gcgccgcttc tggcgcagcg ccgtggccgg
     2761 actctcgccc atgccgatga cgtcgggggc gtccacgaag gtcacgctcg ccggccggcc
     2821 gcggcggtac tcggcgatct cggcctccag gcgtggcacg ttgccgacca ggatcacctc
     2881 gtcggcgttg gtggctgcgt agttgatggc gccgcgtacg gcttcgcgcg gcgcgaagtc
     2941 gccgcccatc gcgtcaagcg cgatgcgcat cgacggcgct cagatcaacg ggagccgccc
     3001 tcgggaggcg tgctctccac cggcacgacc tcgcggccat cgtagtagcc gcaggtcgga
     3061 cagacgtggt gggcgcgctt cagctcgtga cagtgcgtac actcgacgag cggcggcgcg
     3121 ctgatcgcca ggtgcgaccg tcgtcggccc tggcgtgcct tggatacttt tcgcttgggg
     3181 acgcccacgc gtgactcctg gagctcgatt caaagaggcg ggacgacgca gtatagccgc
     3241 gcaccggtat cggcgcgaat tcgcccgctc agtcctcgtc gcgctcaagg agctgctgca
     3301 gcgccgccag gcgcgggtcg atctccggct cgccatggct tggatggccg ggggccagcg
     3361 gctcgccgca ctccgggcac aggccggcgc agttggggtc gtggagcggc accatcggct
     3421 cggtcagggc gaactcctcg cgcaggaccg gcccgaggtc gatctcgttg tgctcgtcga
     3481 tggcccgcac ctcctcgtcc gcggacggct cgggcagcgc ggcccccgac tctggatcga
     3541 ggctgggcag gtactcctcc gaaagctgga tgcgggccgg ctccacaaac ggctccaggc
     3601 atcgggcgca ggtgcgccgc acgggagcct ccaggtcgcc gcgcaccagg atgccgcggt
     3661 tggtgcgctg catgcggatg gtgccgttga tcggtccggc cagctccacg tcaggcccca
     3721 gggacacgta tcggtcgcgc aggcggtgtc cgcgtaccgc cccgggtggc tcccggagca
     3781 ggcccgccac gttgaagacg gtcactgccg ggtagcggcc tcggtcgcct ccggctcgcc
     3841 gcttgcgtcg tgcggaacgt cgttggtgct tggacgcacg cggtggcgct cgtcgagcat
     3901 gtcgatcccg cgcttgatgc tggtcagggc cttgatgcat tccccctcca gccggatcag
     3961 gacgccggcg gcgtactcgt cggcgccgcg ccgcacctcg gccgcctcgt ggttggcggc
     4021 gtccaggatc tcgcccgcgc gctgctgcgc catgcgcacc agctcccgct cctcgagcat
     4081 ctgcgcggcc tgttcctggg cacgggcgat gatggcgtca ccctcctcgc gcgcccgctc
     4141 ggtgatacgc cccgcctcct cggtgatgcg cttcgcctgg cgcacctcgt cgggcacggc
     4201 gacgcggagc tggtcgatca gctccagcac ggctgcctgc tccagcacga tgttgttcgt
     4261 gaggggcagc ttcttgcccg cggcgaccag gctctcaagc cgctcgacga ggaagatgat
     4321 gtccgtggcg tgtgccctcc ctcaggtggc gcgattatag acctgccccg ttcatgctct
     4381 gcggctgtgc ggctgtgcgg ctgtgcggct gtgctgcacg aagggccatg acagcggtcg
     4441 gtggtgccgg cggaccaccg cgcgggcgtc aggccccggt tgtgcccctg tgcggctgtg
     4501 cggcacagac tggcactgac ccccggctat cccttcgcga agcgggcctg cagtgcctcc
     4561 gccaccggtc ccggcaccat gccggacacg tcgccgccga actgggcgac ctccttggcc
     4621 agc
//
LOCUS       DATACA010000401         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1176883, whole genome shotgun sequence.
ACCESSION   DATACA010000401 DATACA010000000
VERSION     DATACA010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176883"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..317)
                     /locus_tag="VHK28_08245"
     CDS             complement(15..317)
                     /locus_tag="VHK28_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2142243.1"
                     /translation="MPEQRRKFSPQFKAEAVQMVLETGKPIAEVARDLGCSDQTLRNW
                     VAQAAIDAGEREGLTSDERAELARLRRENRLLREEREILRKAAAFFAQETMSGGSR"
     gene            complement(414..1640)
                     /locus_tag="VHK28_08250"
     CDS             complement(414..1640)
                     /locus_tag="VHK28_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018804005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2142244.1"
                     /translation="MWFVGDDWAEDHHDVEVQDGTGRRLARARLPEGVAGIARLHELI
                     AGVVAEEAEPGQIAIGIETDRGPWVAALVAAGYRVFAINPRQVARYRERHGTSGAKSD
                     AADAHTLADMVRTDAHQLRPVAGDSALAEGVKVLTRAHQNLIWDRHRQMLRLRAALRE
                     FFPAALEAFPDLTAPDALELLAAAPDPDRAARLSRSKIAAALRRARRRDVEAKAVEVQ
                     AALRSAQLTQPAELAGAYAATVRASVALIAAINTQITALGKQVEAHFGRHPDAEIYRS
                     QPGLGDLLGARVLGEFGDDPDRYAGARARKNYAGQAPITRASGKKRVVLARYVRNRRL
                     ADALHQQAFCALRASPGARAYYDCLRARGAGHHAALRQLANRLVGILHGCLKTRTLYD
                     ETTAWAHHTKNQRAAA"
BASE COUNT          288 a          747 c          717 g          330 t
ORIGIN      
        1 gatcagccgg cagatcacct cgaaccgccg ctcatcgtct cctgggcgaa gaaggccgct
       61 gcctttcgca ggatctcccg ctcctcgcgc agcagccggt tctcacggcg aaggcgggcc
      121 agctcggccc gctcgtcgct cgtcaggccc tcacgctcgc cggcgtcgat cgccgcctgg
      181 gcgacccagt tgcgcagcgt ctgatccgag cagcccagat cgcgggcgac ctcggcgatc
      241 ggcttgccgg tctcgagcac catctgcacg gcttcagcct tgaactgcgg actgaacttc
      301 ctccgctgct cgggcatgga cgcatcctct cagttcagag gacgtgtcca agatcgttgg
      361 tacaccccaa agctcggcgg tgtaggtgac agacatccca tgagctggag atgtcaagcg
      421 gcggctcgtt gatttttggt gtggtgtgcc caggcggtgg tctcgtcata gagggtgcgg
      481 gtcttcaggc agccgtgcag gatgccgacg aggcggttgg ccagctgacg gagggcggcg
      541 tggtgcccgg cgccgcgggc gcgcaggcag tcgtagtagg cgcgggcgcc cggtgaggct
      601 ctcagcgcgc agaacgcctg ctggtgcagg gcgtcggcca gccgccggtt gcgcacgtag
      661 cgggcgagga cgacgcgctt cttgcccgag gcacgggtga tcggggcctg tccggcgtag
      721 tttttccggg cccgggcccc ggcgtagcgg tcggggtcgt caccgaactc gccgagtacc
      781 cgggcgccga gcaggtcacc gaggccgggc tggctgcggt agatctcagc gtccgggtgc
      841 cggccaaaat gagcctccac ctgcttcccg agagcggtga tctgagtgtt gatcgcggcg
      901 atcagcgcca cgctcgcgcg cacggtcgcg gcgtaggcgc cggccagctc cgccggctgg
      961 gtcagctgcg ccgagcgcaa cgcggcctgg acttccacgg ctttggcctc cacatcgcgg
     1021 cggcgggctc ggcgcagtgc cgcggcgatc ttcgaccgcg acagccgggc cgcccggtcc
     1081 ggatccggcg cggcggccag cagctccagg gcgtccgggg cggtcaggtc ggggaaggcc
     1141 tccagcgcgg cgggaaagaa ctcccgcagc gccgcgcgca gccgcagcat ctgccggtgc
     1201 cggtcccaga tcaggttctg atgcgcccgg gtgagcacct tgacgccctc ggccagcgcg
     1261 ctgtccccgg cgaccggccg cagctgatgg gcgtcggtgc gcaccatgtc ggccagggtg
     1321 tgggcgtcgg cggcgtcgct cttggcgccg gaggtgccgt gccgctcgcg gtagcgggcc
     1381 acctggcggg ggttgattgc gaacactcgg tagccggccg cgaccagcgc ggccacccac
     1441 gggccccggt cggtctcgat cccgattgcg atctgcccag gttcggcttc ctcggcgacc
     1501 acgccggcga tcagctcgtg cagccgggcg atcccggcca ccccttcggg cagccgtgcc
     1561 cgcgccagcc gacggccggt accgtcctgc acctccacgt cgtggtgatc ttcggcccag
     1621 tcgtcgccga cgaaccacat cccgccctct cccacctgcc gcacggatca cggtcggcag
     1681 cctgcgggag atcaccgcgg cctaatggac cagtgctcgc gccacgatca gcggacacgt
     1741 catcccatca gcggtcaggc tcccgaccac cggtgagggc acggtctgtc actaggactc
     1801 accaactcct ggatcggaga gtgctcaccc accggcggct accagcaccg agccttccgc
     1861 atccacatcc gggcccggca gcccccatta ggatcggaca cgccacgacc tcggtgcggc
     1921 tggacatctg tgagaggacg tccctcctac cgaggtcctg cgcttgtccc ggtcatctga
     1981 ccgccctcgg cgtgtccctg acctgcctgc ctcgactact gggaatggct catggagcac
     2041 ctcacgggct gagacgccat ccacgtcgcc tacgtcgttg ac
//
LOCUS       DATACA010000402         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2140693, whole genome shotgun sequence.
ACCESSION   DATACA010000402 DATACA010000000
VERSION     DATACA010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2140693"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VHK28_08255"
     CDS             complement(<1..483)
                     /locus_tag="VHK28_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142245.1"
                     /translation="MSGSKRPSTSSLRRFITSRPYVTVAELRRRFALDDPEAMAHIRR
                     NGTAAWIGLPDREASKVQDLWERDEVGVELSVEVRAPVVVGIYPRRIARYVTDGGNGD
                     VTAPNGNGAANDAPNGIGEHTEAHDTARGWQRNREVTRGHLPRYPGYRRQPDPPSAPP
                     P"
     gene            complement(544..1476)
                     /locus_tag="VHK28_08260"
     CDS             complement(544..1476)
                     /locus_tag="VHK28_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2142246.1"
                     /translation="MLAASLRVVFIWLSNRRWMARLAMGLPLVRSLARRFVSGTSLDE
                     AVEAVRALNERGAMATIDVLGESVDDRSTAERAAASYVTTLERIATEGLDANVSLKLT
                     QMGMDLGLDTCLEVLRPVVEAGRRHGIFVRVDMESSAYTDRTLEVVRRLRADGHDVGA
                     VLQSYLHRSRTDAAALAAEGCRVRVCKGAYAEPEAIAYQDPAEINRSFVDCMVTLLEA
                     DAYPAAATHDPEMIAAVRAAAEERGIGPDRFEFQLLYGIGRDIVDRLLANGYQVRIYV
                     PYGEQWWPYYMRRLAERPANVMFFLRGLFGRRGR"
     gene            complement(1494..1838)
                     /locus_tag="VHK28_08265"
     CDS             complement(1494..1838)
                     /locus_tag="VHK28_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX2142247.1"
                     /translation="MRVELLYWDGDAEYMTARQRLVEVLTEDAFETPIQMISVNSPSD
                     AVLLHFPGSPTIRIDGSDIYPPGATERGLRLRHYPPDEDLDGPATEPVPGKRLIRRAV
                     ERARGWGHGRHD"
     gene            complement(1846..2112)
                     /locus_tag="VHK28_08270"
     CDS             complement(1846..2112)
                     /locus_tag="VHK28_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142248.1"
                     /translation="MELLWLASAVAFAVAAYVVGWPAWASYRAREARDLNAERYLAWR
                     GRASSPGTTSTSEGMTGQERRRVVLGAALAVVALVSLFGFFVAT"
     gene            complement(2123..2869)
                     /gene="pgl"
                     /locus_tag="VHK28_08275"
     CDS             complement(2123..2869)
                     /gene="pgl"
                     /locus_tag="VHK28_08275"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX2142249.1"
                     /translation="MTRSFRVLPDENAAYTAAADAIVAAGQESIRERGRFVIALSGGS
                     TPRAVCPMLVVPPRVQMVDWSKVEFFFGDERAVPPNDPDSNYNTAREALLNYLPGLDR
                     DRVHRMPCELEDLEAGARQYEAEIAETLGVRPPQLPVFDLVWLGMGPDGHTASLFPGS
                     EGLEERERWVVSHYAPGPDAWRMTLTFPVLNAAREALFVVTGAEKADAFRAIRGGSRE
                     YPAGRIDAQRTTWLVDAAAAGEAPVAASPG"
     gene            complement(2866..4122)
                     /locus_tag="VHK28_08280"
     CDS             complement(2866..4122)
                     /locus_tag="VHK28_08280"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX2142250.1"
                     /translation="MTTAPREIALHRRLMRFGEAESIVAPLWTASDTSVREVEAHLAR
                     LWSMSRRDDGAVVVEKGLPHSRASVLNLIVTVPDASAADRVVGTMLGLGFRHPSRAIV
                     LVAEPEADRPSVDATVSAHCHPSNGGAGDEVCYEEVILTVHGEAAANLNGVVAPLLIH
                     DLPTMVWWPGDPPFVNPIFDQLVELSDRLIVDSSDFSDLLLGMRRMVSLRRRSGIGDL
                     SWKRLGWWQELTAQFFDAPRFRRYLPNLNRVRIQYALTDGTDEDGTDADGVRSPIAQA
                     LLYAGWVASRLEWRRHATPDPLAAGSLRLTLEGRYEMVDLQIEGVRRDDVPAGELVSA
                     RLRAHGETGAAEFIVDRHGDEATVASNADGMTALLRNVRMELPSESELLAASLLMDRH
                     DALYESALRAATIFLASAQRPDEDRS"
     gene            complement(4119..5621)
                     /gene="zwf"
                     /locus_tag="VHK28_08285"
     CDS             complement(4119..5621)
                     /gene="zwf"
                     /locus_tag="VHK28_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX2142251.1"
                     /translation="MPEPCTMVICGATGDLTERKLAPALYNLGLGGFLPPEFTMVGMA
                     RRDLTDEQFQDHLKAGIDRFSRNRPAKPSIWESFCRGIFYHRASFDDPAAWQELSALL
                     DRIDRDRGTTGNRLFYLAVPPALYPQIVRQLGDAGLAEPDAKRGGRRGWARVIVEKPF
                     GYDLASAQTLNSELRRTFDEEQIYRIDHYLGKETVQNLSVFRFGNGLFEPIWNRRYIE
                     SVQITVAETVGVEGRGEFYDQTGALRDIVQNHALQLLAMFAMEPPVEFTAQDLRDEKL
                     KVLRGVRPMSPDEVGRDTVRGQYVSGWVEGEKMPSYRDEPEVAPDSETETYVALRLGI
                     ESWRWAGVPFYIRTGKALSTRVTEVAVQFKRPPLALFERAGAPQIDPNILAIRIQPDE
                     GILIRFGAKVPGQGMQIRSVNMDFRYGSSFAVDSPDAYETLLLDAMIGDASLFTRGDE
                     VERAWEILDPILEAWKAGEGGRIHFYGAGTWGPPAADDLLERDGRSWRRP"
     gene            complement(5755..>6189)
                     /locus_tag="VHK28_08290"
     CDS             complement(5755..>6189)
                     /locus_tag="VHK28_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HEX2142252.1"
                     /translation="PAGARHYVKMVHNGIEYGIMQAYGEGFDILHASDYTLDLQAIAR
                     MWQNGSVIRSWLLELAGNAFEKEGNDLESIRGYVNDSGEGRWTVFDAIDHDVPAPVIT
                     LSLIQRFRSRRDPDSFSDRVLAALRNEFGGHAVQSFAGDESP"
BASE COUNT          898 a         2172 c         2191 g          928 t
ORIGIN      
        1 gggaggcggg gccgacggcg gatcgggctg ccgccggtag cccgggtagc gtgggaggtg
       61 gccgcgcgta acttcccggt tccgctgcca tcctcgtgcg gtgtcatgtg cctcggtgtg
      121 ctcaccgata ccgttcggtg cgtcgttcgc tgcgccgttc ccattcgggg ccgtgacatc
      181 tccgttgccg ccatcggtca cgtagcgagc gatgcgccgc gggtagatgc ccaccaccac
      241 cggtgctcgc acctcgaccg agagctccac accaacctcg tcgcgctccc agaggtcctg
      301 caccttgctg gcctcgcggt ccggcaggcc gatccaggcc gccgtgccgt tgcggcggat
      361 atgggccatc gcctctggat cgtccagcgc gaaccggcgc cgcagctcgg cgaccgtgac
      421 gtacgggcga gaggtgatga atcggcgcag gctcgaggtg ctcggtcgtt tgctgccgct
      481 catcgttggg catccatggc tgaacccgag tctaccccca tgtctccccc gccgcagggc
      541 ggcctaccgg ccgcgccggc cgaacaggcc gcgcaggaag aacatcacgt tggccggccg
      601 ctcggccagg cggcgcatgt agtacggcca ccactgctcg ccgtacggga cgtagatccg
      661 gacctggtag ccgttggcca gtaaccggtc gacgatgtcc cgcccgatgc cgtacagcag
      721 ctggaactcg aagcggtccg ggccgatccc gcgctcctct gcggccgcgc ggaccgctgc
      781 gatcatctcc gggtcgtggg tggccgccgc cgggtaggca tccgcctcca ggagggtgac
      841 catgcagtcc acgaaggagc ggttgatctc cgcggggtcc tggtaggcga tcgcctctgg
      901 ctcggcgtag gcacccttgc acactcgcac ccgacagccc tccgccgcca gggcggcggc
      961 atcggtcctg gaccgatgca ggtaggactg cagcacggcg cccacgtcgt gcccatccgc
     1021 acgcaggcgc cgcacgacct ccagggtacg gtccgtatag gccgatgact ccatgtccac
     1081 ccgcacgaag atgccgtgcc gccggccggc ctcgaccacc gggcgcagca cctccaggca
     1141 ggtgtccaga ccgaggtcca tccccatctg ggtcagcttc aggctgacgt tggcgtccag
     1201 gccctccgtg gcgatgcgtt ccagggtggt cacgtaggag gcggctgccc gctcggccgt
     1261 ggagcggtca tcgacgctct cgcccaggac gtcgatggtg gccatggcgc cgcgctcgtt
     1321 cagggcgcga accgcctcga ccgcctcgtc caggctggtg ccggacacga agcggcgcgc
     1381 caggctgcgg accagcggca ggcccatggc cagccgcgcc atccagcgac ggttggagag
     1441 ccagatgaag acgacccgca ggctggcggc gagcacctct cgttgcggcc cgactagtcg
     1501 tgccggccgt ggccccatcc gcgcgcgcgc tcgacggcgc ggcgaatcag gcgtttgccc
     1561 ggcaccggtt cggtggctgg tccgtcgagg tcctcgtcgg gcgggtagtg gcgcagccgc
     1621 aacccgcgct cggtcgcgcc gggcggatag atgtccgatc cgtcgatgcg gatggtgggc
     1681 gagcccggga agtggagcag cacagcgtcc gagggcgagt tgacgctgat catctggatg
     1741 ggcgtctcga aggcgtcctc ggtcagcacc tccaccagcc gctgtcgggc ggtcatgtac
     1801 tcggcgtcgc cgtcccagta gagcagctcc acgcgcatgg acgggtcagg tggccacgaa
     1861 gaagccgaac agggagacga gggccaccac ggcgagcgcc gcgcccagca caactctgcg
     1921 tcgttcctgg ccggtcatgc cctcgctggt cgacgtcgtc cccgggctgg aggcgcggcc
     1981 gcgccaggcc aggtagcgct cggcgttgag gtcacgcgcc tcgcgggcac ggtacgaggc
     2041 ccaggccggc cagccgacga cgtaggcagc cacggcgaag gccacggccg acgccagcca
     2101 cagcagctcc atgggccata gcctagcccg gggacgccgc caccggtgcc tctcccgcgg
     2161 cggcggcatc caccagccag gtggtgcgct gggcgtcgat gcgcccggcg ggatactcgc
     2221 gcgacccgcc acggatggcg cggaaggcat ccgccttctc agcgccggtg accacgaaca
     2281 gcgcctcccg ggccgcgttc agcacgggga aggtgagggt catgcgccac gcgtccgggc
     2341 cgggcgcgta gtgggacacc acccatcgct cccgctcctc cagcccctcc gacccgggga
     2401 agagcgaggc agtgtgcccg tccgggccca tccccagcca gacgaggtcg aagaccggca
     2461 gctgcggcgg gcgcacgccc agggtctcgg cgatctccgc ctcgtactgt cgcgcgccgg
     2521 cctccaggtc ctcgagctca cacggcatgc ggtggacgcg gtcccggtcg aggccgggca
     2581 ggtagttgag cagtgcctcg cgggccgtgt tgtagttcga gtcggggtcg ttggggggca
     2641 ccgcgcgctc gtcgccgaag aagaactcga ccttcgacca gtcgaccatc tgcacccgcg
     2701 gcggcacgac cagcatgggg cacaccgcgc gcggcgtgga gccgccggac agggcgatca
     2761 cgaagcgccc gcgctcgcgg atggactcct ggccggcggc cacgatggcg tcggcggccg
     2821 cggtataggc ggcgttctcg tcgggtagca cccggaagct gcgcgtcacg aacgatcctc
     2881 gtctgggcgt tgagcgctgg ccaggaagat ggtagccgcc cgcagcgccg actcgtagag
     2941 cgcgtcgtgg cggtccatga gcaggctggc ggccagcagc tccgactcgc tgggcagctc
     3001 catgcgcacg ttgcgcagca gggcggtcat gccgtcggcg ttggacgcga ccgtggcctc
     3061 gtcgccgtgg cggtcgacga tgaactcggc ggcgcccgtc tcgccatgtg cccgcagccg
     3121 ggcgctcacc agctcgccgg caggcacgtc gtcacgtctc acgccctcga tctggaggtc
     3181 caccatctcg tagcggccct ccagggtaag gcgcagcgag ccagccgcca gcgggtcggg
     3241 cgtcgcatgc cggcgccatt cgaggcgtga cgcgacccag ccggcgtaga gcagtgcctg
     3301 ggcgatcggg ctgcgcacgc cgtctgcgtc ggtcccgtct tcatcggtcc cgtcagtcag
     3361 ggcgtactgg atgcgtaccc ggttcagatt gggaaggtag cgtcggaagc gcggcgcgtc
     3421 gaagaactgg gccgtcagct cctgccacca gcccaggcgc ttccacgata ggtcgccaat
     3481 ccccgagcgg cggcgcagcg acaccatgcg gcgcatgccg agcagcaggt ccgagaagtc
     3541 gctgctgtcg acgatgagcc ggtccgacag ctcgaccagc tggtcgaaga tcgggttgac
     3601 gaagggcggg tcgccgggcc accagaccat ggtcggcagg tcgtggatca gcagcggcgc
     3661 caccactccg ttcaggttgg cggccgcctc gccgtggacg gtgaggatga cctcttcgta
     3721 gcacacctcg tcgccggccc cgccgttcga cggatggcag tgcgcgctga ccgtggcatc
     3781 cacggacggc cgatcggcct ccggctccgc gaccagcacg atcgcccgcg atgggtggcg
     3841 aaagccgagg ccgagcatgg tgcccaccac ccggtcggcg gcggaagcgt ccggcacggt
     3901 cactatcagg ttgaggaccg atgccctcga gtgcggcagc cccttctcca ccacgaccgc
     3961 cccgtcgtcc cggcggctca tgctccacag ccgggcaagg tgcgcctcga cctcgcggac
     4021 cgatgtgtcg cttgccgtcc acagcggagc gacgatggac tccgcctcgc cgaagcgcat
     4081 caggcggcgg tgcagcgcga tctcgcgcgg ggcggtggtc acggccggcg ccaggacctg
     4141 ccgtcccgct ccagcaaatc gtcggcggcc ggtggacccc aggtgccggc cccgtagaag
     4201 tggatgcggc caccctctcc cgccttccag gcctccagga tgggatccag gatctcccag
     4261 gcgcgctcga cctcgtcccc ccgggtgaag agcgaggcgt cgccgatcat cgcgtccagc
     4321 agcagcgtct cgtaggcgtc cggcgagtcg acggcgaacg acgacccgta tcggaagtcc
     4381 atgttcacgc tgcggatctg catcccctgt cccggaacct tggccccgaa ccggataagg
     4441 atgccctcgt cgggctggat gcgaatggcc aggatgttgg ggtcgatctg cggcgctccg
     4501 gcgcgctcga agagtgccag cggcggccgt ttgaactgca ccgccacctc cgtcacccgc
     4561 gtgctgagcg ccttgccggt gcggatgtag aacggcacgc cggcccagcg ccacgactct
     4621 atgcccagcc tgagcgccac gtaggtctcg gtttccgagt caggggcgac ctccggctcg
     4681 tctcggtagg agggcatctt ctcgccctcg acccagccgg agacgtactg cccgcggacc
     4741 gtgtcgcggc cgacctcgtc gggcgacatg gggcgaacgc cgcgcagcac cttgagcttc
     4801 tcgtcgcgca ggtcctgggc cgtgaactcc accggcggct ccatggcgaa catggccagc
     4861 agctgcaggg cgtggttctg gacgatgtcg cgcagggcgc cggtctgatc gtagaactcg
     4921 ccgcggccct cgacgcccac cgtctcggcc acggtgatct gcaccgactc gatgtagcgc
     4981 cggttccaga tgggctcgaa cagcccgttg ccgaagcgga agacgctcag gttctggacc
     5041 gtctccttgc cgaggtagtg gtcgatgcgg tagatctgct cctcgtcgaa ggtgcgacgc
     5101 agctcgctgt tcagcgtctg ggcactggcc aggtcgtagc cgaacggctt ttcgacgatg
     5161 acccgcgccc acccccggcg gccgccgcgc tttgcgtccg gctccgccag gccggcatct
     5221 cccagctgcc tgacgatctg cgggtagagg gccggcggca ccgccaggta gaagagccgg
     5281 ttgccggtcg tcccgcggtc acggtcgatg cggtccagca gcgccgagag ctcctgccag
     5341 gcggccgggt cgtcgaagct ggcgcggtgg tagaagatgc cccggcagaa ggactcccag
     5401 atggagggct ttgccggccg gttacggctg aagcggtcga ttcccgcctt gaggtggtcc
     5461 tggaactgct cgtccgtcag gtcgcgccga gccatgccga ccatggtgaa ctcgggcggc
     5521 aggaagccac cgaggcccag gttgtagagc gccggggcga gcttgcgctc ggtcaggtca
     5581 ccggtcgccc cgcagatgac catggtgcac ggttccggca tgcgctcggt gcgcagcccc
     5641 tcacgcagcg ggttgggcgc agtgcccggc acgaccgcgt gcggcggcgc cgaggccggg
     5701 cttccggcgc ccacggtggc ggtctgggtg cggctgcgag cggtcgacat cggtctacgg
     5761 ggattcgtcg ccggcgaacg attgcaccgc atggcccccg aactcgttgc gcagcgcggc
     5821 gagcacccgg tcgctgaagg agtcggggtc acgccggctg cggaagcgct ggatgagcga
     5881 cagggtgatg accggcgccg ggacgtcgtg gtcgatggcg tcgaacaccg tccagcggcc
     5941 ctcgccggag tcgttcacgt aaccgcggat cgactccagg tcgttgccct ctttctcgaa
     6001 ggcattgccg gccagctcca gcagccagga gcggatgacc gacccgttct gccacattct
     6061 ggcgatcgcc tggaggtcca gcgtgtagtc ggaggcgtgc aggatgtcga agccctcgcc
     6121 gtaggcctgc atgatcccgt actcgatgcc gttgtggacc atcttgacgt agtggcgggc
     6181 gccggccgg
//
LOCUS       DATACA010000403         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1739584, whole genome shotgun sequence.
ACCESSION   DATACA010000403 DATACA010000000
VERSION     DATACA010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739584"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VHK28_08295"
     CDS             <1..1696
                     /locus_tag="VHK28_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX2142253.1"
                     /translation="ERLKKIIEELRSSSDSVLFIDELHTLVGAGAAEGAIDAANILKP
                     PLARGELQCIGATTLDEFRKYIEKDAALERRFQPVMVEEPTVDEAVAILFGIRSRYEE
                     HHKVKITDEAVHAAVDLSVRYVADRALPDKAIDLIDEAGSRVRLRKASAPPEIREAQK
                     ALEEVTRQKDEAIANQDYEAAARLRDEEAQGKERIEEMRASWHEESAQDVPEVTEEDI
                     AQVVSMWTGIPVIRISAEESERLLHMEEALHKRVVGQQEAIDIISKAVRRARAGLKDP
                     KRPIGSFIFMGPTGVGKTELAKALAEFMFGSEDALVKIDMSEFMERHNVSRLVGAPPG
                     YVGYDEGGQLTEAVRRKSYSVVLLDEIEKAHPEVFNILLQILEDGHLTDAKGRRVDFR
                     NTVIVMTSNVGAQLLAKDTTIGFRAGANEALQAAQDYDRMKEKVLTELKNVFRPEFLN
                     RIDATVVFRQLTREEIREIVDMLLLRVKNQLTGQQMDLVVTDAAKDAIIAKGYDQAYG
                     ARPLRREIQNQIEDPLAERMLMASFESGDTIVVDLAEDGSLSIEKGSAEREPVKAG"
     gene            1789..3165
                     /gene="radA"
                     /locus_tag="VHK28_08300"
     CDS             1789..3165
                     /gene="radA"
                     /locus_tag="VHK28_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX2142254.1"
                     /translation="MPTVRSIYVCQQCGAESPKWSGQCPSCQGWNTLVETAGVRTPVG
                     PGRARARASQVAAASVSPIGEVSSAEADRLPTGIGEVDRVLGGGFVPGSIVLFGGDPG
                     IGKSTLLLQVAARLAVGGQSVLYLTGEESTSQVRGRAERVGAVVDGLSLLATTELETA
                     VGAIGGTSPALAVVDSVQTMASGDLSGPAGSVGQVREAATRLAEVAKAGVTCVALVGH
                     VTKEGTVAGPRTLEHLVDAVIYLEGERLGATRLIRAAKNRFGSTDEVGVLEMRGDGLV
                     EVADASRFFLESEQPPVAGSAVTIALEGTRPLAVEIQALVAPSGAGSPRRATTGIDVN
                     RVLMLIAVLARHAGLNLTGHDVYVNVAGGLRIEEPSADLAVAAALASSLRERPIRTGT
                     VLAGELSLSGRLRPAPRAERRLAEAGRLGFTRLVTGASSADGRDPADGLLRLPDIRSA
                     LREILATE"
BASE COUNT          519 a         1113 c         1212 g          453 t
ORIGIN      
        1 ggagcgcctc aagaagatca tcgaggagct gcgatccagc agcgacagcg tgctcttcat
       61 cgacgagctg cacaccctgg tgggtgccgg cgccgcggag ggtgccatcg acgcggccaa
      121 catcctgaag ccgccgctgg cgcgcggcga gctgcagtgc atcggtgcca ccacgctgga
      181 cgagttccgc aagtacatcg agaaggatgc ggcgctcgag cggcgcttcc agccggtcat
      241 ggtcgaggag ccgacggtcg acgaggcggt cgccatccta ttcggcatcc gcagccggta
      301 cgaggagcac cacaaggtca agatcactga cgaggcggtc cacgccgccg tcgacctctc
      361 cgtgcgttac gtggccgacc gcgccctgcc ggacaaggcc atcgacctga tcgacgaggc
      421 cgggtcacgc gtgcggctgc gcaaggcatc ggccccgccg gagatccgcg aggcccagaa
      481 ggccctggag gaggtcaccc gccagaagga cgaggcgatc gccaaccagg actacgaggc
      541 cgctgcccgg ctgcgcgacg aggaggcgca gggcaaggag cgcatcgagg agatgcgcgc
      601 cagctggcac gaggagtcgg cgcaggacgt gccggaggtg accgaggagg acatcgccca
      661 ggtcgtcagc atgtggaccg gcatccctgt catccgcatc agcgcggagg agtcggagcg
      721 cctgctgcac atggaggagg ccctccacaa gcgggtcgtc ggccagcagg aggcgatcga
      781 catcatctcc aaggcggtgc gccgggcgcg cgccggtctc aaggacccca agcgacccat
      841 cggctcgttc atcttcatgg gccccaccgg cgtcggcaag accgagctgg ccaaggcgtt
      901 ggccgagttc atgttcggct ccgaggacgc cctggtgaag atcgacatga gcgagttcat
      961 ggagcgccac aacgtgtcgc ggctggtcgg tgcgcctcca ggctatgtcg gctacgacga
     1021 gggcgggcag ctgaccgagg cggtgcgccg caagagctac agcgtggtgc tgctggacga
     1081 gatcgagaag gcgcacccag aggtgttcaa catcctcctc cagatcctgg aggacggcca
     1141 cctgaccgat gccaagggcc gccgcgtgga cttccgcaac accgtgatcg tgatgaccag
     1201 caacgtcggt gcgcagctgc tggccaagga cacgaccatc ggcttccggg ccggtgccaa
     1261 cgaggcgctg caggcggcgc aggactacga ccgcatgaag gagaaggtgc tcaccgagct
     1321 gaagaacgtt ttccgcccag agttcctgaa ccggatcgac gccacggtcg tcttccgcca
     1381 gctgacccgc gaggagatcc gcgagatcgt ggacatgctc ctgctgcggg tgaagaacca
     1441 gctgacgggg cagcagatgg acctggtggt gaccgatgcc gccaaggacg cgatcatcgc
     1501 caagggctac gaccaggcct acggtgcccg cccgctgcgg cgcgagatcc agaatcagat
     1561 cgaggacccg ctggcggagc gcatgctgat ggcctcgttc gagagcggcg acacgatcgt
     1621 cgtcgacctg gccgaggacg gcagcctcag catcgagaag ggttcggcgg agcgcgagcc
     1681 ggtcaaggcc ggataacgga ccgatacgtc ggcggtaagc cggcgcgtgg caacatgacg
     1741 gacggcgagc cattcggccg ccgtccgttc gcttgtcttc ggtgaccgat gcccaccgtc
     1801 cgctcgatct acgtctgcca gcagtgcggc gccgagtcgc cgaagtggtc cggccagtgc
     1861 ccgtcctgcc agggctggaa cacgctcgtc gagaccgccg gggtccgcac gcctgtcggc
     1921 cccggccgcg ctcgcgcgcg ggccagccag gtggccgcgg cctcggtgtc accaatcggt
     1981 gaggtgagca gcgcggaggc cgaccggctg ccgaccggta tcggcgaggt ggaccgcgtg
     2041 ctcggcggtg ggtttgtgcc cggctcgatc gtgctgttcg gcggcgatcc gggcatcggc
     2101 aagtccaccc tgctgctgca ggtggctgcc cggctggccg tgggtgggca atccgtgctc
     2161 tacctgaccg gcgaggagtc cacttcgcag gtacgcggcc gggcggagcg ggttggggcc
     2221 gtggtcgacg gcctgagcct gctggccacc accgagctcg agacggcggt gggcgccatc
     2281 ggcggcacct ctccggcgct ggccgtggtg gacagcgtgc agaccatggc cagcggggac
     2341 ctctcggggc cggccggcag cgtggggcag gtgcgggagg ctgccacccg gctggccgag
     2401 gtggccaagg cgggtgtcac ctgcgtggcg ctggtcgggc acgtgaccaa ggaggggacc
     2461 gtggccgggc cgcggaccct ggagcacctg gtcgacgcgg tgatctacct ggagggggag
     2521 cggctgggcg ccacgcgcct gatccgcgcc gcaaagaacc gctttggctc cacggatgaa
     2581 gtcggcgtgc tcgagatgcg cggcgacggg ctggtggagg tggccgatgc cagccgcttc
     2641 ttcctcgaat cggagcagcc gccggtggcg ggcagcgcgg tgaccatcgc cctggagggc
     2701 acgcggccgc tggccgtcga gatccaggcg ctggtggctc cctccggcgc cggctcgccg
     2761 cgccgggcca ccaccgggat cgacgtcaac cgcgtcctga tgctgatcgc ggtgctcgcc
     2821 cgccacgccg ggctgaacct gaccggccac gacgtgtacg tcaacgtggc cggcggcctg
     2881 cgcatcgagg agccgtcggc tgacctggcg gtggcggccg cgctggcctc cagcctgcgc
     2941 gagcgcccca tccggaccgg caccgtgctg gccggggagc tctcgctgtc cgggcggctg
     3001 cgtccggcgc cgcgcgccga gcgccggctg gcggaggccg gccggctggg cttcactcgc
     3061 ctggtgaccg gcgcatcgag cgctgacgga cgcgaccccg ccgacgggct gctgcgcctg
     3121 ccggacatcc gctccgcgct gcgcgagatc ctggccaccg aatagctcgc tcgccctccg
     3181 gccattgccc gccaattcag cgccgtgcta aggtgaccgc ccgccgctag gcgggcgcga
     3241 ggccacgggg cgtcatcggt tcattcgcat tcatcttccc gcccgacggc agcgcac
//
LOCUS       DATACA010000404         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1017753, whole genome shotgun sequence.
ACCESSION   DATACA010000404 DATACA010000000
VERSION     DATACA010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017753"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1561
                     /locus_tag="VHK28_08305"
     CDS             209..1561
                     /locus_tag="VHK28_08305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004988388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX2142255.1"
                     /translation="MPAPNMEVVVSLAKRRGFVFPSSEIYGGMGGFWDYGPLGVELKN
                     NVKAAWWRAMVQLRDDVVGLDSAIVMNPRVWEVSGHVGGFSDPMVDCRSCKHRFRADD
                     LKGPPSEIACPNCGQRGTLTEARQFNLMFQTHVGPVEESASVAYLRPETAQGIFVNFD
                     NVATTTRLKLPFGIAQIGKSFRNEITPGNFIFRDREFEQMEMEYFVHPSEEDRWFGYW
                     VEERMRWWTQDIGISSERLRLRPHDTDELSHYSRQTTDVEYDFPMGWSELEGIADRTD
                     YDLAAHSSASGKGLTIFDEASGEHVTPYVIEPAMGVDRAVLAVLLDAYEEQQLEKEKR
                     VVLHLRPALAPIKVAVLPLLRNRPELVERARALAADLRPCMAAMYDDTASIGKLYRRQ
                     DEIGTPFCVTVDVDSLEDGKATIRERDSMTQQRVSLDLIPERLAALVAGSAPWEPGSD
                     "
     gene            1628..2374
                     /locus_tag="VHK28_08310"
     CDS             1628..2374
                     /locus_tag="VHK28_08310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2142256.1"
                     /translation="MTDTAFDELRAAAEGGDNESVLLLTERMLADNPGNDAAHEVRAR
                     ALLALGRVDEAEAHAADAVRLDPEEIRYRELMAEVLAAEGAHREAAAEYGRLARNDPG
                     QAAWTVAEAQERLGASQPGHSVEAARRAVRLDPRNAEAQLALARGLLLTGEARNALQA
                     ASVAAELLPGDRRARETLADARWLADQDSAAFAEFRALAHELEGSDQDRVIGKARRLY
                     LQHAGFLGRLLAGIGPVFRVALTRGWIGVR"
     gene            2371..>2603
                     /locus_tag="VHK28_08315"
     CDS             2371..>2603
                     /locus_tag="VHK28_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142257.1"
                     /translation="MTTGREPETPGPATTTGVLRDHLAAALSSAMRRPEAEAIALTAD
                     RTKAMAVAMAGRPDDAPVDVRSLELSTRQAATVL"
BASE COUNT          400 a          930 c          906 g          367 t
ORIGIN      
        1 cccagaacct ctctagtgcg atcaccattc gcacatgagg gtggaataca cccgcacccc
       61 cgataacgcg atcaccattc gcacgtgagg cggcagccgg cttggtcact cgctgcagcg
      121 cgtgccggtg tctgcgctag actcggaagc tggccgagcc cgcccatcgg tccatctgca
      181 gcccgcaccg gaggagcctt cgacagccgt gcctgctccg aacatggagg tcgtcgtctc
      241 gctcgccaag cggcggggat tcgtctttcc ctccagcgag atctacggcg gcatgggagg
      301 cttctgggac tacggcccgc tgggcgtgga gctcaagaac aatgtcaagg ccgcctggtg
      361 gcgggccatg gtgcagctcc gcgacgacgt ggtcggcctg gactcggcca tcgtcatgaa
      421 cccgcgcgtc tgggaggtgt ccgggcacgt gggtggcttc agcgacccca tggtcgactg
      481 ccgcagctgc aagcatcggt tccgcgcgga tgacctcaag ggcccgccgt ccgagatcgc
      541 ctgccccaat tgcggccagc gcggcacgct gaccgaggcc cggcagttca acctgatgtt
      601 ccagacgcac gtggggccgg tcgaggagag cgcctcggtc gcctacctgc ggccggagac
      661 ggcgcagggc atcttcgtca acttcgacaa cgtggccacc accacccgcc tgaagctccc
      721 gttcggcatc gcgcagatcg gcaagagctt ccgcaacgag atcactccag gcaacttcat
      781 cttccgcgac cgcgagttcg agcagatgga gatggagtac ttcgtgcacc cctccgagga
      841 ggaccgctgg ttcggctact gggtcgagga acgcatgcgc tggtggacgc aggacatcgg
      901 gatctcgtcg gagcgtctcc gcctccgccc gcacgacacc gatgagctca gccactactc
      961 gcgccagacg accgacgtgg agtacgactt cccgatgggc tggtccgagc tggagggcat
     1021 cgctgaccgg accgactacg acctggcggc ccattcctcc gcctcgggaa aggggctgac
     1081 catcttcgac gaggcgagcg gcgagcacgt cacgccgtac gtcatcgagc cggccatggg
     1141 cgtcgaccgc gcggtgctgg ccgtgctgct ggacgcctac gaggagcagc agctggagaa
     1201 ggagaagcgg gtggtcctgc acctgaggcc cgccctggcg ccgatcaagg tggccgtgct
     1261 gccgctgctg cgcaaccgtc cggagctggt ggagcgggcg cgcgccctgg ctgccgacct
     1321 gcgcccgtgc atggcggcca tgtacgacga caccgcctcc atcggcaagc tgtaccgtcg
     1381 ccaggacgaa atcggcaccc cgttctgcgt gacggtggac gtcgactcgc tggaggacgg
     1441 caaggcaacc atccgggagc gcgacagcat gacccagcag cgggtatccc tcgacctgat
     1501 cccggagcgc ctcgccgccc tggtcgcggg gtcggccccc tgggagccag gttcggacta
     1561 gaccgatgcc tcccgctgcc gtctctcacc attgcggcac gccccttgcc ggcccgagca
     1621 tgcagccatg accgataccg ccttcgacga gctccgcgcg gcggcggagg gtggggacaa
     1681 cgaatccgtg ctgctcctga ccgagcgcat gctggccgac aatccgggta acgatgccgc
     1741 ccacgaggtg cgcgcccgcg ccctcctggc cctcggccgc gtggacgagg cggaggcgca
     1801 cgccgcggat gccgtgcgcc tggacccgga ggagatccgc taccgggagc tgatggccga
     1861 ggtcctggcc gcggagggcg cgcaccgcga ggccgccgcc gagtacggtc gtttggcgcg
     1921 caacgaccca ggtcaggcgg cgtggacggt ggccgaggcc caggaacggc tgggtgcctc
     1981 gcagccgggc cattcggtgg aggccgcgcg acgcgcggtg cggcttgacc cgcggaacgc
     2041 ggaggcgcag ctggccctgg cgcgcgggct gctgctgacc ggcgaggcgc gcaacgccct
     2101 gcaggcagca tcggtcgccg ccgagctgct ccccggcgac cgccgcgcgc gcgaaaccct
     2161 ggccgatgcc cgatggctcg cggaccagga cagcgccgcc ttcgccgagt tccgcgccct
     2221 ggcccacgag ctcgagggct cagaccagga tcgggtgatt ggcaaggccc ggcgtctgta
     2281 cctgcagcac gcggggttcc ttggccgcct gctggcgggg atcggtccgg tattccgcgt
     2341 tgccctgacg cgcggatgga tcggcgtgcg gtgacgaccg gtcgcgaacc ggagacgccc
     2401 ggcccggcca ccaccaccgg cgtcctccgc gaccacctgg ccgcggcgct gtcgagcgcc
     2461 atgcgccgtc ccgaagcgga ggccatcgcg ctcaccgccg accgcaccaa ggcgatggcc
     2521 gtggccatgg ccggccggcc ggacgacgcg ccggtcgacg tccgctcgct cgagctcagc
     2581 acccgccagg cagccaccgt gct
//
LOCUS       DATACA010000405         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2303132, whole genome shotgun sequence.
ACCESSION   DATACA010000405 DATACA010000000
VERSION     DATACA010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303132"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VHK28_08320"
     CDS             <1..493
                     /locus_tag="VHK28_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX2142258.1"
                     /translation="VVVSFGSMAMDDAESILRIITSAAAAAGVRAVVQLPGRDGIRVK
                     GQDVLLLHAPVDHRALFPRAAAVVHHGGAGTTHTAAAAGVPQVVVPHVGDQPYWAARL
                     QALGVATPPLPATRLAPHPLAERLRSALHDGELRASARRLGALVKADRGVATAIGLLE
                     GLG"
     gene            651..1625
                     /locus_tag="VHK28_08325"
     CDS             651..1625
                     /locus_tag="VHK28_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2142259.1"
                     /translation="MRVLLAYDGSACADAARDLVARLPLPAGSTISMVTVLARGPELF
                     GAPDFAIVPADASEPESVLVAGLQEMLGQAAAPLRTTERRVDTRVLRGRPASAILQEA
                     EALRPDLIVVGSRGHGPLATMVLGSVSTEIVDHASCPVLVARHPAVRRAVVAVDGSTS
                     AERAIAALRRWPILAAIPTRVVAVGPSAGQLPSAVSSPLSPSRPLLDEPNVDRERERL
                     GAAARWAADELREAGMPVSVDLRAGDPAQEIIASAEQEGANLIVIGSRGLSALPRLLL
                     GSVARKVLLHAPQSVLVVRPARERAERRQPARERSAIPAGALGAFGVA"
     gene            1782..>2180
                     /locus_tag="VHK28_08330"
     CDS             1782..>2180
                     /locus_tag="VHK28_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142260.1"
                     /translation="MRRRIIATLAATALSAAFASTALAGGPPAVGFYVDDALYRTIGT
                     PTAFSRTGAPAHSFDTIYALGGELLNVAEAKPGDRDFNGGRWMVLPVEWHVTPYQLTS
                     AEAVVAAEDRGELTIGSTPVRMFECPVIKLQ"
BASE COUNT          265 a          793 c          788 g          334 t
ORIGIN      
        1 ggtcgtcgtc tccttcgggt cgatggccat ggacgatgcc gaatcgatcc tgcgcatcat
       61 cacctcggcg gctgccgctg ccggggtgcg cgcggtcgtg cagctccccg ggcgcgacgg
      121 cattcgagtg aagggacagg atgtcctgct cctccacgca ccggtcgacc accgtgcgtt
      181 gttcccgcgg gccgcggcag tggtgcacca cggtggtgcg ggcacgaccc acaccgccgc
      241 ggcggccggc gtgccccagg tggtcgtgcc gcacgtcggc gaccagccct actgggccgc
      301 tcggctgcag gcgttgggcg tggcgacccc gcccctgccg gcgacgcgcc tggcgccgca
      361 tcccctcgcg gagcgcctgc gctccgcgct gcacgacggg gagctgcgag cgtcggcgcg
      421 ccgcctcggc gccctggtga aggcggaccg tggtgtcgcg acggccatcg gtctattgga
      481 gggcctgggc taggcaggtt cacctcggcc ggtcccgagg gactggatgt cacgatcgaa
      541 cggttccgat ggcccttcac cgactcggtg catacgaccg atcctgagcg tgcacggcca
      601 ttgcccgcgc ccagaggagg gcggcggacc ggccggcagg aggtgtcgag atgcgtgtgt
      661 tgctcgccta cgacggctcg gcctgcgccg acgccgcccg tgacctggtc gcgaggctgc
      721 cgctcccagc cgggagcact atcagcatgg tgaccgtgct ggcccgcggg ccggagctgt
      781 tcggagcgcc ggacttcgcg atcgtgcccg ccgatgcctc cgagccggag agcgtgctgg
      841 tggccggtct ccaggagatg ctcggccagg ccgccgcgcc gttgcgcacc accgagcggc
      901 gggtggatac ccgtgtgctg cgtggccgtc cggcgagcgc cattctgcag gaagccgagg
      961 cactgcggcc ggacctcatc gtggtcggct cccgcggcca cggcccactg gccaccatgg
     1021 tcctgggctc cgtctcgacc gagatcgtcg atcacgcctc gtgcccggtc ctggtcgcgc
     1081 gccacccggc cgtgcgccgc gcggtggtgg ccgtggacgg gtcgaccagc gcggagcggg
     1141 cgattgccgc gctgcgccgc tggccgatcc tggccgccat cccgacccgg gtggtggctg
     1201 tcggcccctc ggccgggcag ctgccgtcag cggtgagttc gcccctgtcg ccgtctcgtc
     1261 ccctgctgga cgagccgaac gtggatcgcg agcgggaacg cctgggcgcg gcggcacgct
     1321 gggccgcgga tgagctccgc gaggcgggga tgccggtcag cgtcgacctg cgcgccggtg
     1381 atcctgccca ggagatcatc gcctccgccg agcaggaggg cgcaaacctg atcgtgatcg
     1441 ggtcgcgtgg gctgtccgcc ctgccacgcc tgctgctcgg cagcgtggcg cgcaaggtcc
     1501 tgctccacgc tccccaatcg gtgctggtcg tacgccccgc cagggagcgg gccgagcggc
     1561 ggcagcctgc ccgggaacga tcggccatcc cggccggcgc gctgggtgcc ttcggagtcg
     1621 cctgagcagt ttcctgcacc gaatcttcac gattacgtca gcgacccgag acccttgggt
     1681 cgctggccgt tcatggccgg tggccagcat ggacggtggc cggcgatagc gccggtccgg
     1741 gggtcctgcc tccgtccgct gaccttcgcg aggagattgc catgcgacga cgcatcatcg
     1801 ccaccctggc cgccaccgca ctttccgcgg ccttcgcttc gaccgccctg gccggcggcc
     1861 cgccggcggt cgggttctac gtcgacgatg ccctgtaccg aaccatcggc acgcccaccg
     1921 ccttcagccg aaccggtgct ccagctcaca gcttcgacac catctacgcc ctgggcggcg
     1981 agctgctgaa cgtggcggag gcgaagcccg gggaccgcga cttcaacggc ggccgttgga
     2041 tggtgctgcc ggtggaatgg cacgtaaccc cctaccagct gacctcggcc gaggcggtgg
     2101 tcgccgcgga ggaccgtggc gagctgacca tcggctccac gccggtgcgc atgttcgagt
     2161 gcccggtcat caagctccag
//
LOCUS       DATACA010000406         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2704067, whole genome shotgun sequence.
ACCESSION   DATACA010000406 DATACA010000000
VERSION     DATACA010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704067"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1365)
                     /locus_tag="VHK28_08335"
     CDS             complement(223..1365)
                     /locus_tag="VHK28_08335"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2142261.1"
                     /translation="MFRSSLRLFSIGGVEVGVHFSWLLIFALLTWSLATGFFPQAFRE
                     LTGEALADVNAWLLGGLAALLLFVSVLVHELAHSFMARARGLEAKSITLFLFGGVSNL
                     SAESPNPGTEFLVAVVGPLTSLAIGGICFLAAIFVTEPQANLVLAYLAVVNALVAGFN
                     LIPGFPLDGGRVLRSIAWLLTGSVRRATEIAATAGQIVGFGFIVWGLAILFLPPGDDF
                     FSGIWIAAIGWFLQNAASMSLQQVVLETGLRDVRVGTILRRDSTATSPNTDLDELVEV
                     LLRSNRRAMPVVDGARLVGIITTGDISKVPADVRGRTSVSAAMSGGDDLRTVGPTTSL
                     TDALRALGEGDYDQLPVVEDGTLLGMLSRADVIRQLQLREALDAER"
     gene            1454..>1651
                     /locus_tag="VHK28_08340"
     CDS             1454..>1651
                     /locus_tag="VHK28_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020112398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX2142262.1"
                     /translation="MPAVTAENITVLPRLPEPDPLTSVDRPIKSVTLAPRGFEGEGFP
                     VRRAFHGVDLGDLDPFIHMDQM"
BASE COUNT          285 a          564 c          550 g          252 t
ORIGIN      
        1 tgcttgatga gccgctgcgc ctccgcgatc ggatgctcct ttgacagccg cttctcggcc
       61 tccacgatgg cctgctggat ggcgatcgcc tcgtccagct cgtgcttgat gaactcctga
      121 ccggtcttca tggcataccc cgtgacggtt gcgttcatgg atggatggcg gcgcagtgcg
      181 cacggcgtgt gccaactagg tccgcccagt gcggcgaacc ctttatcgct cggcgtccag
      241 cgcctcccgc agctggagct ggcggatgac gtcggcgcgg gaaagcatcc ccagcagggt
      301 tccgtcctcg acgaccggca gctggtcgta gtcgccctcg cccagggcgc gcagcgcatc
      361 ggtcagcgag gtggtgggtc cgacggtgcg caggtcgtcg cctccgctca tggcagccga
      421 gaccgaagtc cggccccgca catccgccgg gaccttgctg atgtcgccgg tggtgatgat
      481 cccgaccagc cgggccccgt cgaccaccgg catcgcccgg cggttgctcc gcagcaggac
      541 ctcgaccagc tcgtcgagat ctgtgttcgg gctggtcgcg gttgagtcgc gtcgcaggat
      601 ggtcccgacc cgcacgtcgc ggaggcccgt ttcgaggacg acctgctgca ggctcatgct
      661 ggccgcgttc tgcaggaacc agccgatggc ggcgatccag atcccgctga agaagtcgtc
      721 gccgggcggc aggaacagga tggccagccc ccacacgatg aacccgaagc cgacgatctg
      781 tccggccgtc gccgcgatct cggtcgcacg ccgcacgctg ccggtcagga gccaggcgat
      841 ggagcgcagg acacggccac cgtccagcgg gaagcccgga atcaggttga agccggccac
      901 cagggcattc accacggcga ggtaggcaag caccaggttg gcctgcggct cggtgacgaa
      961 tatcgctgcc aggaagcaga tgccgccgat cgccaggctg gtgagcggcc cgaccacggc
     1021 gaccaggaat tccgtgccgg ggttcggcga ctcggcgctg aggttcgata cgccgccgaa
     1081 caggaacagg gtgatcgact tggcctccag gccgcgcgcg cgtgccatga aggagtgcgc
     1141 caactcgtgc accaggaccg atacgaagag cagcagcgct gccaacccgc cgagcagcca
     1201 ggcattgacg tcggcgagcg cctcacccgt gagctcgcgg aaggcctgcg ggaagaagcc
     1261 ggtggccagc gaccaggtga ggagcgcgaa gatcagcagc cagctgaagt gaacgcccac
     1321 ctccacgccg ccgatgctga acagccgcag cgagctccgg aacatgtcgc tgacaggagt
     1381 gcgcaatcgc cgtgccgggg ccgggcgaac tccaactaat tgcacgtgca attactcatg
     1441 ctatgattgt tccatgcctg ctgtcaccgc cgagaacatc accgtcctgc cccgcctgcc
     1501 cgagcccgat ccgctcacga gcgtcgaccg acccatcaag agcgtgacgt tggcgccgcg
     1561 cgggttcgag ggcgagggat tccccgtgcg ccgcgccttc cacggtgtcg acctagggga
     1621 cctcgacccg ttcatccaca tggaccagat g
//
LOCUS       DATACA010000407         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2543751, whole genome shotgun sequence.
ACCESSION   DATACA010000407 DATACA010000000
VERSION     DATACA010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543751"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VHK28_08345"
     CDS             <1..879
                     /locus_tag="VHK28_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX2142263.1"
                     /translation="WKRWVEATLDRKNEPVIDVPVSQENVARWADGLDIGVGGETVLF
                     VDCEAAFYRTSVARSVAQLLKMAGVEFGLMKEQWCCGGPAAEMGYTDASRQFAEHNVA
                     DWRAVGAKRIITLDPHDYITFTEDYVDYFGEQHDFEILHITELVAELVERGDLALTKP
                     IERVATYHDPCRLNKRKGIYQSPRAILRAIPGLVFKDVDHVTQWSYCSGAGAGFAIER
                     PEIAAEISRRRVARAKDLEVDLLVSACVWSERPLSEQGAQQGIEVRDLMELVAEAAGL
                     DPRGVSSAGETTEVPA"
     gene            876..2393
                     /locus_tag="VHK28_08350"
     CDS             876..2393
                     /locus_tag="VHK28_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2142264.1"
                     /translation="MTITAMDSVAEELRAILGADGVLTGLSARYNRTRVPAPFPVHRW
                     AEYVPDLVVLPTTAEQVSEVVKLANRERIPVVARAGGTGLNDGALPLKRGIVVDVKRM
                     NQIKELDLVDRTVTVGPGINMLKLNEELRKHGVMYPDDPASYPCSLVGGRIGTSGWSL
                     IGGRYGHTRDLVISFQMVLPTGEIIEIGDGGGRKIRKSSTGYQLKHLFMGHQGTLGIV
                     TEATLELVPRPEVEFAAFFHYPSFTAAWESTGALATSGLASLAGVVLFDEYKLRYLRR
                     DDEAYIPRPDNVKAVVATAMYGTRDEVEPAARRLLRIGRESGGTYLGDEISSGDWASR
                     HDRYATPLHGRLRSGEVVPMSWHCEDASINYSQLPAVMEKWHAIAARYIEKTGILDDW
                     GLFAYTNAAYKPWGDYLTEIDIGIWEQKLDDENWTDWVACKREIAQVALDHGGSITAC
                     HGATREGDAELVPVEMGGGFEVMMKIKAALDPNNIMNPGKYELDRAYGLNEKVFR"
     gene            2390..2650
                     /locus_tag="VHK28_08355"
     CDS             2390..2650
                     /locus_tag="VHK28_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142265.1"
                     /translation="MIGFRLSAQKPPEPKRVTPDVVMRFDHVYEVDPAKMTIIEQQEM
                     PAWDTLRIVDSRWEHLAWMHAHWADSVLVAEDLGEGGPHPDE"
BASE COUNT          508 a          962 c         1011 g          426 t
ORIGIN      
        1 tggaagcggt gggtcgaggc aaccctcgat cgcaagaacg agccggtcat cgacgtgccc
       61 gtctcgcagg agaacgtggc ccgctgggcg gacggcctgg acatcggggt gggcggcgag
      121 accgtcctgt tcgtggactg cgaagcggcc ttctaccgca ccagcgtggc ccgctcggtg
      181 gctcagctgc tgaagatggc gggcgtcgag ttcggcctga tgaaagagca gtggtgctgc
      241 ggcgggccgg ccgcggagat gggctatacg gacgcctccc gccagttcgc cgagcacaac
      301 gtggccgact ggcgagccgt cggcgccaag cggatcatca cgctcgaccc gcacgactac
      361 atcaccttca ccgaggacta cgtcgactac ttcggcgagc agcacgactt cgagatcctg
      421 cacatcacgg agctggtggc cgagctggtg gagcgcggcg acctggcgct gaccaagccg
      481 atcgagcgcg tggcgaccta ccacgacccc tgccggctga acaagcgcaa gggcatctac
      541 cagtcgccaa gggccatcct gcgcgccatc ccgggtctcg tcttcaagga cgtcgaccat
      601 gtcacgcagt ggtcctactg ctctggggcc ggcgccggct tcgccatcga gcggccggag
      661 atcgccgccg agatcagccg gcgacgggtg gcgcgcgcca aggacctgga ggtcgacctg
      721 ctggtatcgg cctgcgtctg gtccgagcgg ccgctgtccg agcagggcgc gcagcagggc
      781 atcgaggtcc gggacctgat ggagctggtg gcggaggccg ccgggctgga cccgcgcggc
      841 gtctcgtccg ccggggagac gacggaggtg cccgcatgac catcacggcc atggattcgg
      901 tggccgagga gctgcgcgcc attctgggcg ccgacggcgt gctcaccggc ctgtcggcgc
      961 gctacaaccg cacgcgggtg cccgccccgt tccccgtcca ccgctgggcg gagtacgtcc
     1021 ccgacctcgt ggtgctgccc accaccgccg agcaggtgag cgaggtcgtg aagctggcca
     1081 accgggaacg catcccggtg gtggcgcggg ccggcggcac cggcctgaac gacggcgccc
     1141 tgccgctgaa gcgcggaatc gtggtcgacg tcaagcgcat gaaccagatc aaggagctcg
     1201 acctggtcga ccgcacggtc acggtgggcc cgggcatcaa catgctcaag ctcaacgagg
     1261 agctgcgcaa gcacggggtg atgtaccccg acgatccagc ttcctacccc tgctcgctgg
     1321 ttggcggccg gatcgggacc agcggctggt ccttgatcgg gggccgatac gggcataccc
     1381 gcgacctggt catcagcttc cagatggtgc tgcccaccgg cgagatcatc gagatcggcg
     1441 acggcggcgg ccgcaagatc cgcaagtcgt ccaccggcta ccagctcaag cacctgttca
     1501 tgggccacca gggaaccctt ggcatcgtga ccgaggccac cctggagctg gtgccgcggc
     1561 cggaggtgga gttcgcggcc ttcttccact acccgtcctt caccgcggcc tgggagtcca
     1621 ccggagccct ggccacctcc gggctggcgt cgctggccgg cgtggtgctc ttcgacgagt
     1681 acaagctgcg ctacctgcgc cgcgacgacg aggcctacat ccccaggccg gacaacgtga
     1741 aggcggtggt ggccaccgcc atgtacggga cccgtgacga ggttgagccg gcggcgcgaa
     1801 ggctgctgcg catcggtcgc gagtcgggcg gcacctacct gggcgacgag atcagctcag
     1861 gtgactgggc ctcgcgccat gaccgctacg ccactcccct ccacggccgg ttgcgaagcg
     1921 gagaggtggt gcccatgagc tggcactgcg aggacgcgtc catcaactac agccagctgc
     1981 cggcggtgat ggagaagtgg cacgcaattg cggccaggta catcgagaag accggcatcc
     2041 tggacgactg ggggctcttc gcctacacca atgccgccta caagccgtgg ggcgactacc
     2101 tgacagagat cgacatcggc atctgggagc agaagctgga cgacgagaac tggaccgatt
     2161 gggtcgcctg caagcgcgag atcgcgcagg tggcgctcga ccacggaggc tcgatcaccg
     2221 cctgccacgg cgccacgcgc gagggagacg ccgaactggt cccggtcgag atgggcggcg
     2281 gattcgaggt gatgatgaag atcaaggccg cgctcgaccc gaacaacatc atgaaccccg
     2341 gcaagtacga gctcgatcgg gcatacgggc tgaacgagaa ggtgttccga tgatcggctt
     2401 tcgcctgagc gcccagaaac cgcccgagcc caagcgggtg acgcccgacg tggtcatgcg
     2461 cttcgatcac gtctacgagg tggacccggc caagatgacg atcatcgagc agcaggagat
     2521 gcccgcctgg gacacgctgc gcatcgtcga ctcccgttgg gagcacctgg cctggatgca
     2581 cgcccactgg gcggacagcg tgctggtcgc cgaggacctg ggcgagggag gaccgcaccc
     2641 cgacgagtga ccctcaccac gggggcgccg ggctaattcc gactgcaggg ccggtagttt
     2701 gacgaggctg actcggatcg ttaggcttgc ctaacttcga acggacgcgg gcacacgccc
     2761 gtgtcaccat cggtccatct gcaggagtga acaagccttg gatctcgggt tcttgacgac
     2821 cggcctgctg accggcctcc gcgagggggt cgaggcggcg ctcatcgtga gcatcatcct
     2881 cgcctacctg gccaagaccg ggaacca
//
LOCUS       DATACA010000408         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2303682, whole genome shotgun sequence.
ACCESSION   DATACA010000408 DATACA010000000
VERSION     DATACA010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303682"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1399
                     /locus_tag="VHK28_08360"
     CDS             353..1399
                     /locus_tag="VHK28_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142266.1"
                     /translation="MARVPLATAGAAFLYAVTAALWLASGNRLPLQDPRIPSDLAPLN
                     GPLGDVLNYALPALAVAGGFIALAVHAVRTPGRLLLASATTFAALYTALVLLVIVDSR
                     ILTLLGYLPYLTINAIMDPGFLASVPEQGWPVLVHQLAAVAGVGLWLVTAAVASRKHS
                     GACLRCGRKHDGGGWLSAASAGKWGTPVTVAAAAIPAVYAIIRFAWAAGIPLGISADF
                     LRQMQENGMVYAGLGLASMAFAGTLLTLGLIQRWGSVFPRWVPLLGGRRVPILLAVIP
                     ASFVAVVVVPAGAELIRLALSGATGGIPFDWTNWGTIGPAALWIFWGPLLGAATLAYY
                     LKRRGSCGRCGRDG"
BASE COUNT          222 a          545 c          514 g          300 t
ORIGIN      
        1 ggaattcggc accacaacgg acgcccggca gacggagccg ccgtcgtcac tctggtctgg
       61 caggaccagc ttggcgcctg gctggtctac gccttcggcc agccggttga cccggcagcg
      121 gagggcggaa ccgcgtgact cccagcatgc tggccgccct gcattacttc aaggcaagct
      181 ccaaccagcc agtccgatga cctcgctccg ccagtcggga gagcgatcac ccgtttgggg
      241 gagcaagtcc gctcggccgg gggatcatcc gcccgcctgc ggcgagaaga ctgaagtcat
      301 gactactcat tcagcacctt cacacccact caaaaccgac gctggcggct tcatggcccg
      361 ggtcccgttg gcgactgcgg gtgcggcttt cctctacgcg gttaccgctg ccctctggct
      421 ggccagcggt aaccggctgc cgttgcagga tccgcgaatt ccctcagact tggcaccgtt
      481 gaacgggccg ctgggcgatg tcctcaacta tgccctcccg gccctcgccg ttgccggggg
      541 cttcatcgcc ctcgcagtcc acgctgtccg cacacccggc cggctgctgc tcgcttcagc
      601 cacaacgttt gccgccctct ataccgcgct ggtcctgctg gtcattgtgg acagccgtat
      661 cctgaccctg ctcggctacc tgccctacct gaccatcaac gccataatgg atccaggctt
      721 cctggcctcg gtgcccgagc agggctggcc ggttcttgtg catcaattgg cggccgtcgc
      781 gggcgtcggg ctgtggctgg ttaccgcagc cgtggcatcg cggaagcatt ccggtgcctg
      841 ccttaggtgc gggcgcaagc acgacggcgg cggctggctt tccgctgcgt cggcaggcaa
      901 gtggggcaca cccgtgaccg ttgccgcggc agccatccct gccgtttacg ccatcatcag
      961 gttcgcctgg gcggcaggaa taccgctcgg catcagcgcg gacttcctgc gtcagatgca
     1021 ggagaacggc atggtctacg ccggcctcgg cctggcatcc atggcattcg ccggtaccct
     1081 cctgaccctg ggcctgatcc agcgctgggg ctcggtcttc ccacgctggg ttccgctgct
     1141 gggcgggcgg cgggttccga tcctgctggc cgtgatcccg gcgtcgttcg tcgccgtcgt
     1201 cgttgtaccg gcaggggcgg aactgatccg gcttgcgctc agcggcgcca ccggcggcat
     1261 tccgttcgac tggaccaact ggggaaccat cggcccggct gcgctctgga tcttctgggg
     1321 tccgttgctg ggagcggcca ccctcgccta ctaccttaag cgccggggat catgtggccg
     1381 gtgcgggcgg gatggctgac cgcggaacga tgttaggcaa gaatagggcc atggcggact
     1441 ccggccggac accggccaaa cgggcagggc aggtcttcag gcactttgtg ttcatgatga
     1501 tcggtgccct gctcgccgtc ccctacatcg cggttttcat ctggggcacg cagcgggctt
     1561 gcttcgggcg agccggccag c
//
LOCUS       DATACA010000409         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_374925, whole genome shotgun sequence.
ACCESSION   DATACA010000409 DATACA010000000
VERSION     DATACA010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374925"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VHK28_08365"
     CDS             <1..1479
                     /locus_tag="VHK28_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2142267.1"
                     /translation="YTRAAVLGMGGSSLAPELFSRVFGGLLGAAPQGSGGMELRILDS
                     THPDAVRGFRSWAQAARTLFCLSSKSGTTTEPNAFHAAMSAFAPALDFLAITDPGTTL
                     AELARAQEFRGIVEAPPDVGGRYSALSVFGLVPAALHGVDVAGLLDRASAMADACRRP
                     PEDNPGLQLGAALGEAALAGRDKLTILTSPRLASFGDWAEQLVAESTGKAGLGIVPVV
                     GEPLGRAAAYGGDRLFVSLTMPGDGDQAINALTAELVDLGHPVERIVIEDPLDVGAEF
                     VRWEVATATAGIVLGIDPFDQPNVQDSKEATRELLEAFRRDGALPEPAPLVAGEGMTV
                     FGEAETLGDQPVSVDGALRTLLSALQPADYLAILAYLPQEPATEAVLQGMRVSVRDAL
                     GPATTLGFGPRFLHSTGQLHKGGPSSGVYLQLTADPSRDLSIPGWDETFGTLVAAQAL
                     GDLQSLQRRGRRVLRIHMADVEAGLARLEAMVGEALESRVPG"
     gene            1506..>2267
                     /locus_tag="VHK28_08370"
     CDS             1506..>2267
                     /locus_tag="VHK28_08370"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEX2142268.1"
                     /translation="MTRQHREERRQQMQIGIAGLGRMGAGMARRLARGDHQVVAWNRT
                     ESVAQELAAEPENKGAVTVAEPLETIAEKLDAPRQVLISVPAGQPTQDMIDQLAGILS
                     PGDVIVDAGNSNFHETRKRAAELEERGIDFLDMGVSGGVWGLEVGFCAMVGGKPEVFE
                     RFEPAVRTLAPEGGYLYCGPAGAGHYVKMVHNGIEYGLMQAYAEGFEILHASEYPLDL
                     AGVAKAWMNGTVIRSWLLELLGLAFEQRGEDLGEIR"
BASE COUNT          326 a          779 c          851 g          311 t
ORIGIN      
        1 tacacccgtg ccgcggtgct gggcatgggc ggctccagcc tggcgcccga gctgttcagc
       61 cgcgtcttcg gcgggcttct cggcgcagcg ccgcaggggt cgggcgggat ggagctgcgc
      121 atcctggact cgacacaccc ggacgcggtg cgcggcttcc gctcctgggc acaggcggcg
      181 cggaccctgt tctgcctgtc gagcaagtcg ggcacgacca ccgagccgaa tgccttccac
      241 gccgccatga gcgccttcgc gccggcactc gacttcctgg ccatcaccga tccgggaacc
      301 acgctggctg agctggcccg cgcccaggag ttccggggca tcgtggaggc gccgcctgac
      361 gtcgggggaa ggtactccgc actgtcggtg ttcggcctcg tcccggccgc gctgcacggc
      421 gtggatgttg ccggcctcct ggaccgggca tccgccatgg ccgatgcctg ccggcgccca
      481 ccggaggaca atcccgggct gcagctggga gccgccctcg gagaggcggc gctggccggc
      541 cgcgacaagc tgaccatcct cacatcgccg cgcctggcca gcttcggcga ctgggcggag
      601 cagctggtgg ccgagagcac agggaaggcc gggctcggga tcgtgccggt cgtcggcgag
      661 ccgctgggac gtgcggctgc ctacgggggc gatcgcctct tcgtgtcgct caccatgccg
      721 ggcgacggcg accaggccat caacgcgctt accgcggagc tggtcgacct gggccatccc
      781 gtcgaacgga tcgtgatcga ggacccgctg gacgtcggcg ccgagttcgt gcgctgggag
      841 gtggccaccg ccaccgccgg aatcgtcctg ggcatcgatc ccttcgacca gccgaacgtc
      901 caggacagca aggaggccac gcgagagctg ctggaggcgt tccggcgcga cggcgccctg
      961 cccgagccgg ccccgctggt cgcaggcgag gggatgaccg tcttcggcga ggcggaaacg
     1021 ctcggcgacc agccggtgag cgtggacggg gcgctccgca ccctgctcag cgcactgcag
     1081 ccagccgact acctcgccat cctggcctac ctgccccagg aacccgccac cgaggcggtg
     1141 ctgcagggca tgcgggtctc ggtgcgcgac gcgctgggac ctgccaccac gctcggcttc
     1201 gggcccaggt tcctgcactc caccgggcag ctgcacaagg gcggtccctc gtccggcgtg
     1261 tacctgcagc tgaccgccga tccgtcccgg gacctgtcca tcccgggttg ggacgagacc
     1321 ttcggcaccc tggtcgcggc ccaggcgctg ggggacctgc agtcgctcca gcggcggggg
     1381 cggcgcgtcc tgcggatcca catggcggac gtcgaggcgg gcctggcgcg cctggaggcg
     1441 atggtcggcg aggcgctgga gagccgcgtg ccgggctgag gcgaattcat cggtccaccg
     1501 cgggtgtgac ccgccagcac agagaggagc ggagacagca gatgcagatc gggatcgccg
     1561 gcctggggcg gatgggagcg ggcatggcac ggcggctggc gcgcggggat caccaggtgg
     1621 tcgcctggaa tcggaccgag tcggtggcgc aggagctggc ggccgagccg gagaacaagg
     1681 gcgcggtcac ggtggccgag ccgctggaga cgatcgccga gaagctcgac gcgccacggc
     1741 aggtcttgat cagcgtcccc gccgggcagc cgacgcagga catgatcgac cagctggccg
     1801 ggatcctctc cccgggagac gtgatcgtgg acgccgggaa ctcgaacttc cacgagaccc
     1861 ggaaacgggc agccgagctc gaggagcgcg ggatcgactt cctggacatg ggcgtcagcg
     1921 ggggcgtgtg ggggctcgaa gtgggcttct gcgccatggt cggcggcaag ccggaggtct
     1981 tcgagcgctt cgagccggcg gtgcgcaccc tggccccgga gggcggctac ctgtactgcg
     2041 gcccggccgg cgccggccac tacgtcaaga tggtccacaa cggcatcgag tacgggctga
     2101 tgcaggccta cgccgaaggg ttcgagatac tgcacgcctc cgagtacccg ctcgacctgg
     2161 ccggcgtggc caaggcctgg atgaacggca ccgtcatccg ctcctggctg ctggagctcc
     2221 tcggactggc cttcgagcag cggggcgagg acctgggcga gattcgc
//
LOCUS       DATACA010000410         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_374930, whole genome shotgun sequence.
ACCESSION   DATACA010000410 DATACA010000000
VERSION     DATACA010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374930"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /gene="gcvPB"
                     /locus_tag="VHK28_08375"
     CDS             complement(<1..1143)
                     /gene="gcvPB"
                     /locus_tag="VHK28_08375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX2142269.1"
                     /translation="MSVAERTPADASGRGAAFSSPASSGPPTVVEPLIFSASVPGRRA
                     VRFPRPSPEAAAAAESQPQVPDAALRADAPRLPEVSELDMLRHFNRLSHLNHAIDLGF
                     YPLGSCTMKYNPKVNEWAARLPGFAESHPLDPDAVAQGSLELEWLLAELLKEISGFAA
                     VSLQPAAGAHGELTGMLMTRAYHRSRGEGEKRTRVLVPDSAHGTNPATATMVGYETVT
                     IRSNARGGIDLQALRAALDESTAALMITNPSTLGIFEDQIDQVVAAVHDVGAIAYMDG
                     ANLNAILGRFRAGEAGFDVMHFNLHKTFSTPHGGGGPGAGPVGVSERMAPFLPAPQVR
                     LAEGDAAEVLSHAWRGRSTQGARFAREDPASRPESIGRVRMHTGNFG"
     gene            complement(1140..>2237)
                     /gene="gcvPA"
                     /locus_tag="VHK28_08380"
     CDS             complement(1140..>2237)
                     /gene="gcvPA"
                     /locus_tag="VHK28_08380"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX2142270.1"
                     /translation="GEVIGRSEFSTAYTPYQPEVSQGTLQSIYEFQSMVCELTGMEVA
                     SASHYDGATATAEAALMACRLTRRDRVAVSTAVHPQVREVLRTYCSGPGIEVVEVPAD
                     LAEGGSGLTTPDAAASLLTDEVACLVAQQPNFFGLIEPMADLADAAHAAGAQLVAYVE
                     PTSLAVLAAPGDYGADIVTAEGQPLGIPLSYGGPYVGLMAARMASVRQMPGRLVGATR
                     DVQGRKGYVLTLQAREQHIRRERAASNICTNQALCALAATAYLSAVGPQGLREVAELS
                     MAQARHLASALESAGAGERRFASPYFAEVAVRVPNAAPRHAALVEEGIVAGYLLERDY
                     PELGDTLLLAATELTTDDDIATLVAALEKDR"
BASE COUNT          300 a          785 c          842 g          310 t
ORIGIN      
        1 gccgaagttg ccggtatgca tcctcacccg gccgatgctc tccgggcggc tggcggggtc
       61 ttcgcgcgcg aaccgggctc cctgcgtgga gcgcccgcgc caggcatggg acagcacctc
      121 cgcagcgtca ccctcggcca ggcgcacctg gggcgccggc aggaagggag ccatgcgctc
      181 gctgacgcct accgggccgg cacctggtcc tccgccacca tgcggcgtgg agaaggtctt
      241 gtgcaggttg aagtgcatga cgtcgaagcc ggcctcgccg gcacggaagc gtcccaggat
      301 ggcgttcagg ttggcgccgt ccatgtaggc gatggcgccg acgtcgtgga ccgcggccac
      361 cacctggtcg atctggtcct cgaagatgcc gagcgtcgat gggttggtga tcatcagcgc
      421 cgcggtcgac tcgtccagtg cggcgcgcag cgcctggagg tcgatcccgc cgcgcgcgtt
      481 ggagcggatg gtgaccgtct cgtagccgac catggttgcc gtcgcggggt tggtgccatg
      541 cgccgaatcc gggaccagca cccgggtgcg cttctcgccc tccccgcggg accgatggta
      601 ggcgcgggtc atcagcatcc cggtcagctc accgtgggcg ccggcggccg gctgcaggct
      661 gacggccgcg aagccggaga tctccttgag cagctcggcc agcagccact cgagctccag
      721 gctgccctgc gccacggcat ccgggtcgag cgggtgcgac tcggcgaagc cgggcagccg
      781 cgccgcccac tcgttgacct tcgggttgta cttcatggtg cacgagccga gcgggtagaa
      841 gcccaggtcg atggcgtggt tcaggtgcga gagccggttg aagtggcgga gcatgtccag
      901 ctcgctcact tccggcagcc gtggcgcatc ggcgcgcagg gccgcgtccg gaacctgcgg
      961 ctggctctcg gctgcggctg ccgcctccgg cgaggggcgc gggaagcgca cggcgcgccg
     1021 cccggggacg gatgcgctga agatgagcgg ctccaccacc gtgggcgggc cggagctggc
     1081 cggcgagctg aaggcggcac cccgcccgga ggcgtcggcc ggcgtgcgct cggcgacgct
     1141 catcggtcct tctccagggc ggccaccagg gtcgcgatgt cgtcatcggt ggtcagctcg
     1201 gtggcagcca gcagcagcgt gtcgcccagc tccgggtagt cgcgctccag gaggtagccg
     1261 gccacgatcc cctcctcgac gagggcggcg tgacgcggcg cggcattcgg cacgcggacg
     1321 gcaacttccg cgaagtaggg cgacgcgaag cgtcgctccc ccgccccggc cgactccagc
     1381 gccgaggcca ggtggcgggc ctgggccatg gacagctcgg ccacctcgcg caggccctgc
     1441 ggcccgaccg cggacaggta ggccgtggcg gccagcgcgc acagggcctg gttggtgcag
     1501 atgttcgaag ccgcgcgctc gcggcggatg tgctgctcgc gggcctggag ggtgaggacg
     1561 tagcccttgc ggccctgcac gtcccgggtg gcgcccacca ggcggcccgg catctgccgg
     1621 acggacgcca tgcgcgcggc catcaggccg acgtacggcc cgccgtagga gagcgggatc
     1681 ccgagcggct gcccctcggc ggtcacgatg tcggcgccgt aatcgcccgg cgcggccagc
     1741 accgccagcg aggtcggctc cacgtaggcg accagctggg cgccggcggc atgcgccgca
     1801 tcggccaggt cggccatcgg ctcgatcagc ccgaagaagt tcggctgctg ggccaccagg
     1861 caggccacct catcggtcag gagggaggct gccgcgtccg gcgtggtcag gcccgatccg
     1921 ccctcggcca ggtcggccgg cacctccacc acctcgatgc ccgggcccga gcagtaggtg
     1981 cgcagcacct cgcggacctg tggatggacg gcggtcgaga ccgccacccg gtcgcggcgg
     2041 gtcaggcggc aggccatcag ggcggcctct gcagtggcgg tggcgccgtc gtagtgcgac
     2101 gcgctggcca cctccatgcc ggtcagctcg cagaccatgg actggaactc gtagatgctc
     2161 tgcagcgtgc cctggctgac ctcgggctgg tagggggtgt aggcagtcga gaactcgctg
     2221 cgaccgatca cctcgcc
//
LOCUS       DATACA010000411         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_616614, whole genome shotgun sequence.
ACCESSION   DATACA010000411 DATACA010000000
VERSION     DATACA010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616614"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VHK28_08385"
     CDS             complement(<1..828)
                     /locus_tag="VHK28_08385"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2142271.1"
                     /translation="MSRLIEIPIRGGRIEAFLMPGGPGVPLLVLGGVETGLRPLAGTE
                     QVLLRRWERRAQRRPVVVVGRPLPDDPADAERLMHPRVMADSVAIALRQVGADGGPQP
                     PFAVEAESGGGRISLWLAVDHPNLVARLVLASVAAETPTDSPMAGRMAQWIGLAEREE
                     WAEFFGKLVVQMRPAEGTEGAASFDAAARLQPRPATPERFIGELKTTLDPSSFVTDRL
                     GEVDVPALVLAGGRDQVVPEASVRLVAERLTNARLEIDPDCGHTVRSNFDGYDDLVEA
                     "
     gene            864..1157
                     /gene="gatC"
                     /locus_tag="VHK28_08390"
     CDS             864..1157
                     /gene="gatC"
                     /locus_tag="VHK28_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX2142272.1"
                     /translation="MISRTDVEHVAALARLGLTDEELDRMQGQLNRILEAINALGAVD
                     TSMVGPTAQVIELENVMREDVSRPGIDREVALANAPLREGDHLRVPVVLEEGR"
     gene            1189..2685
                     /gene="gatA"
                     /locus_tag="VHK28_08395"
     CDS             1189..2685
                     /gene="gatA"
                     /locus_tag="VHK28_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX2142273.1"
                     /translation="MPLADRTIGDVADALRARELTSRELTEACLRRIERDGQKLNTFL
                     AVGAERALAAADEADRALATGEGAVRPLLGIPYALKDIFVTRALDDAGKPDGGLPTTA
                     GSRILEGYRSPYASDAEERLALAGAVLLGKTNCDEFAMGSSNENSAYGPVRNPWDEST
                     VPGGSSGGSAAAVAAGQAFFALGTDTGGSIRQPASLAGIIGLKPTYGRISRFGMIAFA
                     SSLDQCGPLTRSVRDGALVLGALAGHDPRDSTSVNQPVPDYAAALTGEVRGLRLGVPR
                     EFFVAGMEPGVEAAARAAFDVLRDLGAEIVEVSLPSTDAGLATYYIIAPAEASANLAR
                     YDGVKYGLSAGEEDLLDNYLRTRGSGFGPEVKRRIMLGTYALSAGYYDAYYVKAQQVR
                     TLIKAEFDRVLTDVDALLAPTSPSVAFKIGAKTEDPLLMYLNDACTLPVNIAGLPGVS
                     VPCGLSDGLPVGLQVIGRAFDESTILRVADAYERAAGFAALRPPTAHD"
BASE COUNT          428 a         1066 c         1006 g          407 t
ORIGIN      
        1 ggcctcgacc aggtcatcgt agccgtcgaa gttggagcgg accgtgtgcc cgcagtccgg
       61 gtcgatctcc agccgtgcgt tcgtcagccg ctccgccaca agcctgaccg atgcctcggg
      121 caccacctgg tcgcgcccgc cggccagcac cagggccggc acgtcgacct caccgagccg
      181 atcggtcacg aacgaggatg ggtcgagcgt cgtcttaagc tccccgatga agcgctccgg
      241 ggtggccggg cgcggctgca gacgtgcggc cgcatcgaag gacgccgcgc cctcggtccc
      301 ctcggcggga cgcatctgga ccacgagttt gccgaagaac tcggcccact cctcgcgctc
      361 ggcgaggccg atccactgcg ccatccggcc ggccatgggc gagtccgtcg gcgtctcggc
      421 ggccaccgag gcgagcacca gccgcgccac caggttcggg tggtccacgg cgagccacag
      481 cgagatgcgg ccgccgcccg actcagcctc gacggcgaag ggcggctgcg gaccgccatc
      541 ggccccgacc tggcgcaggg cgatggccac actgtcggcc atcacccgcg ggtgcatgag
      601 ccgctcggca tcggccggat cgtcgggaag cggccggccg acgaccacca ccgggcggcg
      661 ctgggcccgt cgctcccatc ggcgcaggag cacctgttcg gtaccggcca gggggcgcag
      721 cccggtctcc acgccgccga gcaccaggag cggcacgccc gggccgccgg gcatcaggaa
      781 ggcctcgatg cgcccaccgc gaatcggaat ctcgatcagg cggctcacga cggcatggtg
      841 ccagagccgc tagcatcggt tccatgatca gccgcaccga tgtcgagcac gtcgccgccc
      901 tcgcccgcct gggactgacc gacgaggagc tcgaccggat gcagggccag ctgaaccgca
      961 tcctggaggc gatcaacgca ctgggcgcgg tcgataccag catggtcggc cccactgcgc
     1021 aagtcatcga gctggagaac gtcatgcgcg aggacgtttc gcgcccgggc atcgatcgcg
     1081 aggtggcgct ggccaacgcg ccgctgcggg agggcgacca cctccgcgtg ccggtggtgc
     1141 tcgaggaggg ccgctagcgc cgtgccgggt accgccgagg ctgccgccct gccgctggcc
     1201 gaccggacca tcggggacgt ggccgatgcg cttcgcgccc gcgagctcac ctcgcgcgag
     1261 ctgaccgagg cctgcctgcg ccgcatcgag cgcgacgggc aaaagctcaa caccttcctg
     1321 gccgtgggcg ccgagcgggc gctggccgcc gccgacgagg cggatcgggc gctggccacc
     1381 ggcgagggtg ccgtgcgccc gctgctcggc atcccgtacg cgctcaagga catcttcgtc
     1441 acccgtgccc tggacgacgc cggtaagccg gacggcgggc tgccgaccac ggccgggtcg
     1501 cggatcctgg agggctatcg gtcgccgtac gcctccgacg ccgaggaacg tttggcgctt
     1561 gccggtgccg tcctgctggg caagacaaac tgcgacgagt tcgccatggg ctcgtcgaac
     1621 gaaaattccg cgtacggacc ggtccgcaac ccgtgggatg agtcgaccgt gccgggcggc
     1681 agttcgggag gctccgcggc cgcagtggcc gctggccagg ccttcttcgc gctgggcacc
     1741 gataccggcg gcagcatccg ccagccagcc tcgctggccg ggatcatcgg cctgaagccg
     1801 acctacggcc gcatcagccg cttcggcatg atcgccttcg cctcctccct cgaccagtgc
     1861 gggccgctga cgcgcagcgt gcgcgacggc gccctggtgc tgggagcgct ggccgggcac
     1921 gatccgcgcg actcgacaag cgtcaaccag cccgtcccgg actacgccgc cgcgctcacc
     1981 ggcgaggtcc gcggcctccg cctgggcgtc ccccgcgagt tcttcgtggc cggcatggag
     2041 ccaggggtgg aggctgccgc gcgggccgcc ttcgacgtgc tgcgtgatct gggcgccgag
     2101 atcgtggagg tctccctgcc gtccaccgat gccgggctgg ccacctacta catcatcgcg
     2161 ccggccgagg cgagcgccaa cctggcccga tacgacggcg tcaagtacgg cctctccgcc
     2221 ggcgaggagg acctgctcga caactacctg cgcacccgcg gctccggctt cggcccggag
     2281 gtcaagcgcc gcatcatgct cggcacctac gccctttcgg ccggctacta cgacgcctac
     2341 tacgtcaaag cccagcaggt gcggacgctg atcaaagcgg agttcgaccg ggtgctgacc
     2401 gatgtggacg ccctgctggc cccaacctcg cccagcgtcg cgttcaagat cggcgccaag
     2461 accgaggacc cgctgctgat gtacctcaat gatgcctgca ccctcccggt caacatcgcc
     2521 gggctgccgg gagtatcggt cccctgcggc ctgtccgatg gcctgccggt cggattgcag
     2581 gtgatcggtc gcgcctttga tgagtcgacc atcctgcggg tggccgatgc ctacgagcgc
     2641 gcggctggct tcgctgccct ccgtccaccc accgcccacg actgaccgtg acggtcggct
     2701 gtgcgcctgt gcggctgtgc ggctgtgccg cacaaacggg gtctgacagc gcgagacccg
     2761 ggtacccctt gacattggag tcgctccagg tcgtagcgtt acgccacgca cacaactcac
     2821 acaggagaac agggatgctg ggtgactttc cgatggcggc ccggctcgcg gcgtcggaca
     2881 tcggtcgggc gagagcctgg tacgagg
//
LOCUS       DATACA010000412        12153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_54191, whole genome shotgun sequence.
ACCESSION   DATACA010000412 DATACA010000000
VERSION     DATACA010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54191"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1515)
                     /locus_tag="VHK28_08400"
     CDS             complement(70..1515)
                     /locus_tag="VHK28_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142274.1"
                     /translation="MTHVDLPPDISGQDLSGAGTVLRNRSFVSLWSAQLLSQLASNMV
                     LAALMATVVSATGSNTANAVLILSFLVPAVVFSAMAGVLVERNDARLILLGANIFRAG
                     GTILFIFVGTNVPLILLINLFIATATAIFAPAELTAIPRIVDRRHLMAANSVFVLTIN
                     ATFAIGFGFLGPLLLTTAGANVVYIVVSAMFGLAALAIIPLPNCPPERRDAIHAEDAT
                     RALRVVVDQVREGLAFVRGHRRIAWSLAYLGIAASLIGVMGAIGPGFATDILRLTPED
                     FFFIMGPAGLGAVMGILFLNGYGKAIPRRLLIDIGLVAMGVTLVALATVKPATDLLAP
                     AVAPIEDTLPAALSPVISLIALVVVIAVTAGVEYAFVAIPSQTALQEELPMEVRGRIF
                     GILNTLLSVASFLPVLVAPAAADLLNIILPGAGIPIVMAALGLVTLWAGIGSWRHNSR
                     AGLHRNQPEPIRPPRPVVEQEPRGTAGHDAE"
     gene            complement(1512..1727)
                     /locus_tag="VHK28_08405"
     CDS             complement(1512..1727)
                     /locus_tag="VHK28_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEX2142275.1"
                     /translation="MAAVELRMGDLIRLRREHPCGSRDWRVVRLGADIGLVCAGCGHR
                     ILMDRLDVERRFTEHLERGPLDEGATP"
     gene            complement(1730..3109)
                     /locus_tag="VHK28_08410"
     CDS             complement(1730..3109)
                     /locus_tag="VHK28_08410"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX2142276.1"
                     /translation="MVAGDVALGVDPYEILAGALERVDGEAEALFLRRDAALTRFANS
                     QIHQNVSEHDASLRVRVVDGERSGVASTNRLDAAGIEDVVERAAELCRRSAANPNATP
                     LAEAAAGGVDSDLGYAASTADADPARRAEGAAAVIAAGRAAGLSVAGAFSTDATTMAV
                     ANTNGLRSRHSSTSAKLLTVMTGEERASGYAQSVSPDIGDIDAAAVGAEAADKAVRSV
                     GAGDVEPGAYDVVLEEYAVQTVLEYLSLAGFSALAVEEHRSFMELGRQIMGANVSVWD
                     DGTDTSGMPAAIDFEGVAKRRVDLVTGGVANAVVHDSATARRAGVESTGHGLPAPNTF
                     GPIAWNLFMAPGDTPRDDLVRRLERGILVTRFHYVNIVHPRRGVLTGMTKDGTFLVEN
                     GKVVRPVRNLRFTQAIPEAFSRIDAIGAETRLVGAEYTGVNARVPALLIRGFNFTGAT
                     ASEAPGTGA"
     gene            complement(3103..4551)
                     /locus_tag="VHK28_08415"
     CDS             complement(3103..4551)
                     /locus_tag="VHK28_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX2142277.1"
                     /translation="MNELIDLALDAAQRAGAAYADVRLSERRTESLTVKNGALQAAST
                     NLSAGFGVRVLVDGAWGFAGSALLERDEVERVTGEALAIARASGLGRVQPVVLDDTPP
                     ATGSYTTPFAEDPFAVSLDDKLAILFEADAAMARVPGVSVREGSIESAREHKIFASSH
                     GGRVEQELVESGAGIEATATNAGDAQVRSYPNSSGGQHVTGGFEAVRAMDLPAHGERI
                     ATEAVQLLDAPECPSGEMTLVIDSSQMALQVHESCGHPIELDRVLGMEASFAGTSFLT
                     LDKLDRLQYGSEIVSIDADATAPGGLGTFGYDDEGVPAQKVPIVSGGRFVGYLSSRET
                     APIIGRRSMGSSRASAWNVIPLIRMTNINLRPGDAGSLDDLIADTDDGLFISTNRSWS
                     IDDRRLNFQFGTQAGWRIRKGKLAEMVRNPTYTGITPRFWASCDAICGPDEWTLWGVP
                     NCGKGEPIQTHRVGHGASPARFRNVQVGVGQW"
     gene            complement(4700..5371)
                     /locus_tag="VHK28_08420"
     CDS             complement(4700..5371)
                     /locus_tag="VHK28_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2142278.1"
                     /translation="MRSPIAQLSLFGVALLIGVLLVGQLRSQARPVEISSLPAPELSE
                     LIQTLAARNRELRTGLADLRETVREYQVAGPQGQSALDVTREELRRITAFTGLAPVHG
                     QGIVLTVSGDLDAIALNDLFHELRNAGAEALGVDEVRVTHRSVAVQGARGLTIDGHDI
                     GSTFTLEAIGSPEGLLATLERPGGIIAQLEQFVDATIVASQSTDIQLPATEQVLTPNV
                     AQSVQ"
     gene            complement(5368..6120)
                     /locus_tag="VHK28_08425"
     CDS             complement(5368..6120)
                     /locus_tag="VHK28_08425"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HEX2142279.1"
                     /translation="MKRRLRPRFGTGWALSVAAALAVVGFVGAAQWNSSVERRDFTTS
                     AQQVLAAQVVELETEQRDLREEIGQAEAQIQEFQEESTSSSVSLAQLNQRLAEARLAA
                     GLTEVRGSGVVIEIADSNRVVPPGENPASFIVLVDDLRDIVTALWASGAEAIAINQER
                     VVATSSIYGVGASILVNTSFLPPPYRITAIGSEGLLDRFMAHDAYRGRVAQRIEFFGL
                     EFATAIEQELTLPPFVGNTRFRWAVPLEEANG"
     gene            complement(6117..7457)
                     /locus_tag="VHK28_08430"
     CDS             complement(6117..7457)
                     /locus_tag="VHK28_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEX2142280.1"
                     /translation="MGRLSGPARRALTALLAAGLLSALLANAAMGFSGFGTTSADGTY
                     GSGMTFEIELRGGAPERLELLLAFSGDDGTFVAPVAADGDSASYTWDTADRHVPPNTE
                     VTYRWRATDGERVTLSAPQTMLYDDDRPGLDWQSEQMGEATVHWYGGAAEQARRFGEL
                     SAEGAAAAEELLGHELAAPVDIFVYDSQEHFFGALGPGAREWTGAATYPHLRTIFMWL
                     QGGSGDFLETTIVHEVTHVVFADATDNPFHEPAKWLNEGLASWSERQSADQERSLVEF
                     EAGGGGLFAFEAITEQFPIGDRGARLSYAQGATMVDMIIDRYGADAIARAAEAYRAGA
                     SDAEALESGTGQPAEDLYAAFYDAFDADAPQPVEPEPIPPSNVRLPGGGETPGQPDGE
                     PAASAAATPDGSGADASDEVPWAVIVAAVLLGVVLIAAAAVLASRRVRDPEETG"
     gene            complement(7445..9031)
                     /locus_tag="VHK28_08435"
     CDS             complement(7445..9031)
                     /locus_tag="VHK28_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX2142281.1"
                     /translation="MPRLTSAGRWPRLAVGIVALVVAIAVALNGTLPSSLPAGAAGPE
                     LRYLGGELKTLDPAYISDAADVQLLLQLYAGLTRLDENGEPYASLAEDWTVSDDGRSY
                     TFRLRDDLSFSDGTPLRAEDVRRSWLRILDPTTATTAPDVLSVIRGADERLAGGSESE
                     VGIQAPDDRTLVVELRHPAAYFPAIAATPTAFVVPPHADASPDWQSVDGFVGSGPYVV
                     DRVDGDTLYLAANRRYVAGSPPIEELRWVMELDGDGATAYADEQLDLVSVPSFDASWI
                     AFDPELGRALHQAAALNVQYFGFDVTAPPFDDPSVRRAFALALDRPRLVELAEGSGST
                     PASSLVPPALWPEGMEDDQQSDPGEARRLLDEAGYRDRSDLGVITVNGGGLGVGPAVA
                     VWREELGVEVVVEGMEFADYLRALEERPPQVFTINWIADYPSPYALYSLLLLPNAASN
                     YGGWTDEDFVRLLEEASSAETDAEQAEAYREVDAHVDEQAPVIPWAYGTSWWLVRPGL
                     RGLGNLTTGILDFGRVSWDG"
     gene            9053..9589
                     /locus_tag="VHK28_08440"
     CDS             9053..9589
                     /locus_tag="VHK28_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142282.1"
                     /translation="MQQPHEHRFRPQPAGSLTLRQFIESLRRWTPEMRQIVQIALTRT
                     EAERQHYLRGFHARHHEREAQKLDSFVRWAYSDPRLDYVPALMADLERTDADYAGAME
                     HEADVLVRMRAEGKTPARRVADYLSLLGIESAEGVREAIARLLPPGTSDEAVTATIRH
                     MRRRWLELYGDDPFFAED"
     gene            9699..11831
                     /locus_tag="VHK28_08445"
     CDS             9699..11831
                     /locus_tag="VHK28_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX2142283.1"
                     /translation="MGPLLRAVLGAVVLMLLLPNGARAHAELVSSTPAANASLSEAPD
                     ELTLTFTEAIDPASATVRLLDPTQAEVDGVGSVSVDSAATTATVPLPDLEAGTYTVSY
                     QVTSAVDGHVTTGLFAFLVDPTGTEPAPSVPTQAESLSSGPEVVAARWLGLIGALAGF
                     GIVLFWLYSARPAYLRARGASELHAPWGALLLVASASVVGLAVYLTLAARPIIASGGH
                     LGHGESFPLDLAAPFGWTPFAIAMRIALAGGVAGFLVAAARWVAHDEARRRGRDAAPG
                     HDGAWLGAAAVASVVTLGGTSLAGHAAAEGGFPFALVDLLHMLGVGAWLGTVPGLFLL
                     AARSRGTVRAALARHSRLALVAAPLVVLTGLASSPLVVGAQPRELLASGYGNLLIAKV
                     LLFCVAVAIGAVNFFLVRGSSVRRVLTLVGIEMTVGALAVLAAAGMVTGQPAANRAPV
                     LTSSAVGAAHLYATAGQSAVHAAINIPAPGSQLYQVAVSDPQTGDPRTDVQRVILVFG
                     PPPGSELSSTRVELEQADEPWLWSARGAYTPVVGDWELEVIVRRIGELDESTTFPLPV
                     IDPLPPQVVPPRDEGVGVPIPLVLLWSVLPDGGLGWVLALALLAGVVAFSIAQRRTPA
                     PPLAVARAGLVVALVVIGLAVGAREAVAIANRAPPDAAAMQNPLEAGEGSIARGRGLY
                     LANCAVCHGTEGAGDGAVGQVEAPSPGD"
     gene            complement(11718..>12153)
                     /locus_tag="VHK28_08450"
     CDS             complement(11718..>12153)
                     /locus_tag="VHK28_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142284.1"
                     /translation="IDPLPPQVVPPRDEGVGVPIPLVLLWSVLPDGGLGWVLALALLA
                     GVVAISIAQRRTPAPALAVARAGLVVALLVIGLAVGAREAVAVANRAPPDAAGMQNPL
                     EASEGSIARGRGLYLANCAVSGALGAVADGAVGQVEAASPGD"
BASE COUNT         1714 a         4375 c         4335 g         1729 t
ORIGIN      
        1 accagggtga agcggatggc cagccacgcc gccagcgcga ccacgaccag gacggctccg
       61 gcgaggagcc tactccgcat cgtggcccgc ggtcccccgg ggctcctgct cgacgaccgg
      121 tcgcggcgga cggatcggtt cgggctggtt gcggtgcagg ccggctcgcg agttgtgccg
      181 ccaggaaccg atgccggccc acagggtcac cagccccagc gccgccatca cgatgggtat
      241 gcctgcgccg ggaaggatga tgttcagcag gtccgccgcc gccggcgcga ccagcactgg
      301 caggaatgac gccaccgaca gcagcgtgtt gaggatgccg aagatgcggc cgcgcacctc
      361 catgggcagc tcctcttgga gggcggtctg cgacgggatg gccacgaagg cgtactccac
      421 gccggcggtc acggctatca ccaccaccag tgcgatcagc gagatgaccg gcgacagcgc
      481 ggccggcagc gtgtcctcga tgggagccac tgccggcgcc agcaggtcgg tggccggctt
      541 gacggtggcc agggccacca gcgtgacgcc catggccacc agcccgatgt cgatcagcag
      601 ccgtcgcggg atggccttgc cgtagccgtt caggaacagg atgcccatca cggcgcccag
      661 gccggccgga cccatgatga agaagaagtc ctccggggtc aggcgcagga tgtcggtggc
      721 gaacccggga ccgatggcgc ccatgactcc gatcaggctg gccgcgatgc ccaggtaggc
      781 cagcgaccag gcgatgcgcc gatggccgcg cacgaaggcc agcccctcgc gcacctggtc
      841 gacgacgacc cgcagggcgc gggtcgcgtc ctcggcgtgg atcgcatcgc gccgctccgg
      901 cggacagttc ggcagcggga tgatcgccag cgccgccagg ccgaacatgg ctgataccac
      961 gatgtacacc acgttggcac cggcggtagt cagcagcaac gggcccagga agccgaagcc
     1021 gatggcgaag gtggcgttga tggtcagcac gaagaccgag ttggcggcca tcaaatggcg
     1081 gcggtccacg atgcgcggga tcgcggtcag ctcggccggc gcgaagatgg ccgtggcggt
     1141 ggcgatgaac aggttgatca gcaggatcag cggcacgttg gtgccgacga agatgaacag
     1201 gatcgtgccg ccggcgcgga agatgttggc tcccagcagg atcagccgtg cgtcgttgcg
     1261 ctccaccagg acgccggcca tggcgctgaa caccacggcc ggcaccagga agctgaggat
     1321 gagcaccgcg ttggcggtgt tcgagccggt ggccgacacc acggtggcca tcagcgcggc
     1381 caagaccatg ttgctcgcca gctggctcag cagctgtgcc gaccacagcg agacgaagct
     1441 gcggttgcgc aggacggtgc ccgccccgga gaggtcctgc ccgctgatgt cgggcggcag
     1501 atcgacgtgg gtcatggcgt ggcaccctca tcgagcggtc cccgctccag gtgttcggtg
     1561 aagcgccgct cgacgtccag gcggtccatc aggatccggt gcccgcagcc ggcgcacacc
     1621 aggccgatgt ccgccccgag ccgcaccacc cgccaatcgc ggctgccgca cgggtgctcg
     1681 cgccgcaggc ggatcaggtc gcccatccgc agctcgacgg cggccaccct cacgctcccg
     1741 tccccggcgc ctcggaagcg gtggcgccgg tgaagttgaa gccccggatg agcaacgccg
     1801 gcacccgtgc gttgaccccg gtgtactcgg cgcccaccag ccgcgtctcg gcgccgatgg
     1861 cgtcgatccg gctgaaggcc tccgggatgg cctgcgtgaa ccgcaggttt cgcaccggac
     1921 ggaccacctt gccgttctcc accaggaagg tcccgtcctt ggtcatgccg gtgaggaccc
     1981 cgcggcgggg atggacgatg ttcacgtagt ggaagcgcgt caccaggatg ccgcgctcca
     2041 gccgccgcac gagatcgtcg cgcggcgtgt cgccgggggc catgaacagg ttccaggcga
     2101 tcggcccgaa ggtgttcggc gccggcaggc cgtgccccgt ggactccacc ccggcgcggc
     2161 gagcagtggc gctgtcgtgc acgacggcgt tggccacgcc gccagtcacc aggtccaccc
     2221 gacgcttggc caccccctcg aaatcgatag cggccggcat gccgctggta tcggttccgt
     2281 catcccagac agatacgttg gcgcccatga tctgcctgcc cagctccatg aaggaccgat
     2341 gctcctcaac cgccagcgcc gagaagccgg ccagcgagag gtactccagg acggtctgca
     2401 ccgcgtactc ctcgagcacc acgtcgtagg ccccgggctc cacgtcgccc gcaccgacgc
     2461 tgcggactgc cttgtccgcg gcttcggcac cgacggctgc ggcatcgatg tcaccgatgt
     2521 ccggtgagac ggactgggcg tagcccgatg cccgctcctc gccggtcatg accgtcagga
     2581 gcttggcgct ggtcgacgaa tggcgcgacc gcagcccgtt ggtgttggcc accgccatcg
     2641 tggtggcatc ggtcgagaag gctccggcca cgctcaggcc tgccgcgcgt cccgcggcga
     2701 tcaccgccgc ggcgccttcg gctcgtcgcg cggggtccgc gtcggccgtg gaggctgcat
     2761 agccgaggtc ggagtccacg ccgccggcgg ccgcctcggc cagtggcgtg gcattcgggt
     2821 tggccgccga gcgacggcac agctccgcgg cacgctcgac cacgtcctcg atgccggcgg
     2881 cgtccaggcg gttggtgctg gcgacgcccg agcgctcgcc gtccaccacc cgcacccgga
     2941 gcgatgcgtc gtgctcgctg acgttctggt ggatctggga gttggcgaag cgggtcagtg
     3001 ccgcgtcacg ccgcaggaag agcgcctcgg cctcgccgtc gactcgctcc agtgcgcccg
     3061 ccaggatctc gtaggggtcg acgcccagcg ccacgtcgcc ggctaccact gccccacgcc
     3121 cacctgaacg ttgcggaagc gcgccgggct ggcgccgtgg ccgacgcggt gggtctggat
     3181 cggctcgccc ttgccgcagt ttggcacgcc ccacagcgtc cactcgtcgg gcccgcagat
     3241 cgcgtcgcag ctggcccaga accgaggggt gatgccggtg taggtggggt tgcggaccat
     3301 ctcggccagc ttccccttgc ggatgcgcca accggcctgc gtcccgaact gaaagttcag
     3361 gcggcggtcg tcgatgctcc acgagcggtt ggtgctgatg aacaggccgt catcggtatc
     3421 ggcgatcagg tcgtccaggc tgccggcgtc gccgggccgg aggttgatgt tggtcatgcg
     3481 gatgagcggg atcacgttcc aggcggacgc ccggctgctg cccatgctgc gacggccgat
     3541 gatgggggcc gtctcgcggc tggagagata gcccacgaag cggccaccgc tgacgatggg
     3601 taccttctgc gccggcacgc cctcgtcgtc gtagccgaag gtgcccagcc cgcccggtgc
     3661 ggtggcatcc gcgtcgatgc tcacgatctc actgccgtac tgcaggcggt caagcttgtc
     3721 cagggtcagg aacgaggtcc cggcgaagct ggcctccatg cccagcaccc gatcaagctc
     3781 gatgggatgg ccgcatgact cgtgcacctg cagcgccatc tgcgaggagt cgatgaccag
     3841 cgtcatctcc cccgacgggc attccggggc gtccagcagc tgtactgcct cggtggcgat
     3901 ccgctcgccg tgggccggca ggtccatggc acgcaccgcc tcgaacccgc cggtgacgtg
     3961 ctggccgccg gaggagttgg ggtaggagcg cacctgcgca tcgccggcgt tggtggcggt
     4021 agcctcgatc ccggcaccgc tctcgaccag ctcctgctcc acgcgtccgc cgtggctgga
     4081 ggcgaatatc ttgtgctccc gggccgattc gatgctcccc tcgcgcaccg agacgcccgg
     4141 tacgcgggcc atggccgcgt ccgcctcgaa caggatggcc agcttgtcat ccagcgagac
     4201 ggcgaaggga tcctccgcga acggcgtcgt gtagctgccc gtggcggggg gcgtgtcgtc
     4261 gagcaccacc ggctggaccc gtcccaggcc gctggcacgg gcgatggcca acgcctcgcc
     4321 agtcacgcgc tcaacctcgt cgcgctccag cagcgccgag ccggcgaatc cccacgcccc
     4381 gtccaccagg acgcggacgc cgaaccccgc agacaggttg gtggaggcgg cctgaagcgc
     4441 gccgttcttc accgtaagcg actcggtacg acgctcggag agccgtacgt ccgcgtaggc
     4501 ggccccggcg cgctgcgcgg cgtcgagggc gaggtcgatc agctcgttca cggttctccc
     4561 ggaggcgcgt cgcggcgtgg agccgctggt ccagaccgcc gttcggcgca catgccgcag
     4621 cggtgagatg acattcgatg ggacgggcgc cactctacgg cctgcccatt ctgacggtca
     4681 aggcgagggc gtgggccgct cactggaccg actgcgccac gttcggggtg agcacctgct
     4741 cggtggccgg cagctggatg tccgtcgatt ggctggccac gatggtcgcg tccacgaact
     4801 gctccagctg ggcgatgatc ccgccgggac gctcgagcgt ggccagcagt ccctccggcg
     4861 agccgatggc ctccagggtg aaggtgctgc cgatgtcgtg cccatcgatg gtcagcccgc
     4921 gggcgccctg gaccgccacg gaccgatggg tgacgcgcac ctcgtccacg cccagcgcct
     4981 ccgcccccgc gttgcgcagc tcgtggaaca ggtcgttgag ggcgatcgcg tccaggtccc
     5041 cgctcacggt gagcacgatg ccctgaccgt ggacgggcgc cagcccggtg aaggcggtga
     5101 tgcgccgcag ctcctcgcgc gtcacgtcca gcgcgctctg gccctgcggt ccggccacct
     5161 ggtactcgcg caccgtctcg cgcaggtccg ccaggccggt gcgcagctcg cgattgcggg
     5221 ccgccagggt ctggatcagc tccgacagct cgggcgccgg cagcgagctg atctcgaccg
     5281 gcctggcctg cgagcgcagc tggccgacca gcagcacgcc gatcagcagg gccaccccga
     5341 acaggctgag ctgggcgatc ggcgacctca tccgttcgcc tcctcgagcg gcaccgccca
     5401 acggaaacgc gtgttgccga cgaagggcgg cagcgtcagc tcctgctcga tcgcggtggc
     5461 gaactccagg ccgaagaact cgatgcgctg ggcgacccgg ccgcggtagg cgtcatgcgc
     5521 catgaatcgg tccagcaggc cctccgagcc gatggcggtg atccggtacg gcggtgggag
     5581 gaacgaggtg ttgaccagga tggaggcgcc cacgccgtag atggaggagg tggcgaccac
     5641 gcgctcctgg ttgatcgcga tcgcctccgc ccccgacgcc cacagcgcgg tgacgatgtc
     5701 gcgcaggtca tccaccagca cgatgaagct ggccggattc tctccgggcg ggaccacccg
     5761 gttcgagtcg gcgatctcga tcaccacgcc cgacccgcga acctcggtca ggccggccgc
     5821 cagccgcgcc tccgccaggc gctggttcag ctgcgccagg ctgacggagc tggaggtcga
     5881 ctcctcctgg aactcctgga tctgggcctc ggcctggccg atctcctctc gaaggtcacg
     5941 ttgctccgtc tcgagctcca cgacctgcgc ggccagcacc tgctgggccg acgtcgtgaa
     6001 atcgcggcgc tcgaccgagc tgttccactg ggccgcgcca acgaagccga ccacggccag
     6061 ggcggccgcc accgagagcg cccagccggt gccgaagcgc gggcggaggc ggcgcttcac
     6121 cccgtttcct ccgggtcgcg cacgcgccgg ctggccagga ccgcggcggc ggctatcagg
     6181 actacgccca gcagcacggc cgcgacgatg accgcccagg gcacctcgtc ggaggcgtcg
     6241 gcgccggaac catccggcgt ggccgccgcc gaggcggccg gctcgccgtc aggctgcccg
     6301 ggcgtctcgc cgccaccggg caggcggacg ttggatggcg gaatcggctc gggctcgacc
     6361 ggttgcggag cgtcggcatc gaatgcgtcg tagaaggcgg catagaggtc ctcggccggc
     6421 tggccggtac cggattccag ggcctcggcg tcggacgcgc cggcgcggta ggcctcggcg
     6481 gcgcgcgcga tcgcgtcggc tccgtagcgg tcgatgatca tgtcgaccat ggtcgcaccc
     6541 tgcgcgtagg agagacgcgc cccacgatcg ccgatcggga actgctcggt gatcgcctcg
     6601 aaggcgaaca gtccccctcc gccggcctcg aactccacca gcgagcgctc ctggtcggcg
     6661 gactgccgct ccgaccatga cgccaggccc tcgttgagcc acttggccgg ctcgtgaaac
     6721 ggattgtcgg tggcgtcggc gaagacgacg tgcgtcacct cgtggacgat cgtggtctcc
     6781 aggaagtcgc ccgatccgcc ctggagccac atgaagatgg ttcgcaggtg cgggtaggtg
     6841 gcggcgcccg tccactcacg cgcgccgggg cccagggcgc cgaagaaatg ctcctggctg
     6901 tcgtacacga agatgtcaac cggcgccgcc agctcgtggc cgagcagctc ctccgccgcg
     6961 gccgctccct cggccgacag ctccccgaag cgcctggcct gttcagccgc gccaccgtac
     7021 cagtgcacgg tggcctctcc catctgctcc gactgccagt ccaggcccgg ccggtcgtcg
     7081 tcgtacagca tggtctgcgg cgcgctgagg gtgacgcgct cgccatcggt cgcccgccag
     7141 cggtaggtga cctcggtgtt gggcgggacg tggcgatcgg cggtgtccca ggtgtaggag
     7201 gcggagtcgc catcagcggc gaccggcgcc acgaacgtgc catcgtcgcc actgaacgcc
     7261 agcagcagct ccagccgctc gggcgccccg ccccgcagct cgatctcgaa cgtcatgccg
     7321 ctcccgtagg tgccgtccgc gctcgtcgtc ccgaagccgc tgaagcccat ggccgcgttg
     7381 gccagcaggg cgctcagcag gccggccgcc agcagcgcgg tcagtgcgcg ccgagccggg
     7441 ccgctcagcc gtcccacgac actctcccga agtccaggat gccggtggtc aggttgccca
     7501 ggccgcgcag cccaggacgc accagccacc aggaggtgcc gtacgcccag gggatgacgg
     7561 gggcctgctc gtccacgtgg gcatcgacct cccggtaggc ctctgcctgc tcggcatcgg
     7621 tctctgcgga ggaagcctcc tcgagcagcc gcacgaagtc ctcatcggtc cagccgccgt
     7681 agttgctggc cgcgttgggc agcagcagca ggctgtacag ggcgtagggc gaggggtaat
     7741 cggcgatcca gttgatggtg aagacttgtg gcggtcgttc ctccagggcg cgcaggtagt
     7801 cagcgaactc catgccctcc accaccacct cgacgcccag ctcctcgcgc catacggcca
     7861 ccgccggtcc cacgcccagg ccgccgccgt tgactgtgat cacgccaagg tcggagcggt
     7921 cccggtagcc agcctcgtcg agcagccgcc tggcctcgcc cgggtcggac tgctggtcgt
     7981 cctccatgcc ctccggccac agcgccggcg gcaccaggct ggaggcgggc gtggagccgg
     8041 agccttccgc cagctcgacc aggcgcggcc ggtccagggc cagggcgaac gcccgtcgca
     8101 cggagggatc gtcgaagggc ggcgccgtga cgtcgaagcc gaagtactgc acgttcagcg
     8161 cggccgcctg gtggagggcg cggcccagct cgggatcgaa ggcgatccaa ctggcgtcga
     8221 aggacggcac gctcaccagg tcgagctgct cgtccgcgta ggcggtcgca ccgtcaccat
     8281 cgagctccat gacccaccgt agctcctcga tgggcggact gcccgccacg tagcgccggt
     8341 tggcggccag gtacagcgtg tcgccgtcga cgcggtccac cacgtacggg ccggacccca
     8401 cgaagccgtc gacgctctgc cagtcgggcg aggcgtcggc gtgcggcgga acgacgaagg
     8461 ccgtcggcgt ggctgcgatc gcgggaaagt aggccgcggg gtggcgcagc tcgaccacca
     8521 gcgtccggtc gtccggcgcc tggatgccga cctccgactc ggagccgccg gccagacgct
     8581 cgtcggcgcc gcgaatcacg ctcagcacgt ccggcgcggt ggtggccgtg gtcgggtcca
     8641 ggatgcgcag ccagctgcgg cgcacgtcct cggcgcgaag gggggttccg tccgagaagc
     8701 tcaggtcgtc ccgcaggcgg aaggtgtagg agcgcccgtc atcgctgacc gtccagtcct
     8761 cggccaggga cgcgtagggc tccccgttct cgtccaggcg ggtcaggccg gcgtatagct
     8821 ggagcagcag ctggacatcg gccgcgtcgc tgatgtacgc cggatccagt gtcttcagct
     8881 cgccaccgag gtagcgcagc tccgggccgg cggcgccggc cggcagcgag gagggcagcg
     8941 tgccattgag ggcgacggcg atggcaacga cgagcgcgac gatcccgacc gcgaggcggg
     9001 gccaccggcc cgctgaggtc aggcggggca tgcgccggta gtataggtgg acgtgcagca
     9061 gccccacgag caccggttcc ggccgcaacc ggccgggagc ctgaccctcc gccagttcat
     9121 cgagtcgctc cgtcgctgga cgcccgagat gcgccagatc gtgcagatcg cgctgacccg
     9181 cacggaggca gaacgccagc actacctgcg cgggttccac gcgcgccacc acgagcgtga
     9241 ggcgcagaag ctcgactcgt tcgtgcgctg ggcctactcc gacccgcgcc tggactacgt
     9301 cccggccctg atggccgacc tcgaacggac cgatgcggac tacgccggcg ccatggagca
     9361 cgaggcagac gtcctggtcc ggatgcgggc cgaggggaag actcccgccc gccgcgtggc
     9421 cgactacctg tcactgctcg ggatcgagag cgccgagggc gtccgcgagg cgatcgcccg
     9481 gctgctgccg cccggaacgt ccgacgaggc ggtcaccgcc accatccgcc acatgcgccg
     9541 ccgctggctg gagctgtacg gggacgaccc cttcttcgcc gaggactgag ctcgccgcct
     9601 ccggggaggc gtgccgctcc ccgtatactc gaacgtagcc ccacgaccga gtgaggaggt
     9661 cagacctcat ccgaccgatc gcccgtgccc gctcaccgat gggtccgctc ctgcgcgccg
     9721 tcctgggtgc cgtggtgctc atgctcctcc tgccgaacgg ggcgcgggcg cacgccgagc
     9781 tcgtctcgtc cacgccggcg gcgaatgcca gcctgtcgga ggcaccggac gagctgacgc
     9841 tgaccttcac cgaggcgatc gatccagcat ccgccaccgt ccggctcctc gatcccacgc
     9901 aggccgaggt ggacggcgtg ggcagcgtca gcgtggactc cgcggcgacc accgccacgg
     9961 tccccctgcc cgatctcgag gccgggacct acacggtcag ctaccaggtc acctcggcgg
    10021 tggacggcca cgtcaccacc ggcctgttcg ccttcctggt cgatcccacc gggacggagc
    10081 cggcgcccag cgtgcccacc caggcggagt cgctctcgtc cgggccggag gtggtcgcgg
    10141 ctcgctggct cgggttgatc ggagcgctgg ccgggttcgg catcgtactg ttctggctgt
    10201 actcggcgcg tcccgcgtac ctccgcgcgc gcggcgcatc cgagctgcat gcgccatggg
    10261 gcgcgctgct gctggtggcg tccgcctccg tcgtggggct ggccgtgtac ctgaccctgg
    10321 cggcgcgacc catcatcgcg tcgggcggcc acctggggca cggcgagagc ttcccgcttg
    10381 accttgccgc gccgttcggc tggacgccgt tcgcaatcgc catgcgtatc gccctggcgg
    10441 gtggggtggc cggtttcctc gtggcggccg cgcgctgggt ggcccacgac gaggcgcgcc
    10501 gccgcgggcg ggatgcggcg ccgggccacg atggcgcctg gctgggcgct gcggcggtgg
    10561 cctcggtcgt gaccctgggc ggcaccagcc tggcgggcca cgctgcggcc gagggaggct
    10621 tcccgttcgc cctggtcgac ctgttgcaca tgctgggggt gggggcatgg ctggggaccg
    10681 tgccggggct gttcctgctg gcggcccgca gccgagggac ggtgcgcgcc gcgctggcgc
    10741 gccactcccg gctggcgctg gtggcggccc cgctggtggt gctcaccggg ctggcgagct
    10801 cgcccctggt cgtcggcgcc cagccccgcg agctgctggc cagcggctac ggaaacctgc
    10861 tgatcgccaa ggtgctgctc ttctgcgtgg cggtggccat cggcgcggtg aacttcttcc
    10921 tggttcgcgg cagctccgtg cgccgcgtgc tgaccctggt cggaatcgag atgaccgtcg
    10981 gcgcgctggc ggtgctcgcc gcggccggga tggtgaccgg tcagccggca gccaaccgcg
    11041 ccccggtgct cacttcgtcg gccgtcggag cggcgcacct gtacgccacg gcgggccagt
    11101 cggcggtgca cgccgccatc aacatcccgg cccccggcag ccagctgtac caggtcgccg
    11161 tctcggatcc gcagaccggc gatccgcgca cggacgtgca gcgcgtgatc ctggtcttcg
    11221 gcccaccgcc gggcagcgag ctttcctcga cccgtgtgga gctggaacag gccgatgagc
    11281 cctggctgtg gtcggcgcgc ggcgcgtaca ccccggtggt cggcgactgg gagctggagg
    11341 tcatcgtgcg ccgcatcggc gagctggacg agtccacgac cttcccgttg ccggtcatcg
    11401 atccgctgcc gccgcaggtg gtgccacccc gcgacgaggg cgtcggggtg cccatcccgc
    11461 tggtgctgct gtggtcggtg ctgccggacg gggggttggg ctgggtgctg gctctcgcgc
    11521 tgctggccgg ggtggttgcg ttctcgatcg cccagcgccg aacgcccgct ccacccctgg
    11581 ccgtggcgcg cgccggcctg gtggtggcgc tggtggtcat cggcttggcg gtcggtgccc
    11641 gcgaagcggt ggccatcgcc aaccgggcgc cgcccgacgc ggccgccatg caaaaccctc
    11701 tcgaagccgg cgagggttca atcgcccggg gacgcggcct ctacctggcc aactgcgccg
    11761 tctgccacgg caccgagggc gccggagacg gcgcagttgg ccaggtagag gccccgtccc
    11821 cgggcgattg agccctcgct ggcttcgaga gggttctgca tgccggccgc gtcgggcggc
    11881 gcccggttgg cgactgccac cgcctcgcgc gcaccaaccg ccaggccgat gaccagcagc
    11941 gccaccacca gcccggctcg cgccacggcc agcgccggag cgggcgttcg gcgctgggcg
    12001 atcgagatcg caaccacccc ggccagcaac gcgagggcca gtacccagcc caaccccccg
    12061 tccggcagca cggaccacag cagcaccagc gggatgggca ccccgacgcc ctcgtcgcgc
    12121 ggtggcacca cctgcggcgg cagcggatcg atg
//
LOCUS       DATACA010000413         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_777955, whole genome shotgun sequence.
ACCESSION   DATACA010000413 DATACA010000000
VERSION     DATACA010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777955"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /gene="dtd"
                     /locus_tag="VHK28_08455"
     CDS             complement(<1..348)
                     /gene="dtd"
                     /locus_tag="VHK28_08455"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2142285.1"
                     /translation="MRLVVQRVTRARVTVDRMVVGDIGRGAVVLVGVGAGDSDATADA
                     MADRLAVLRYFPDDVGRTNLALAEADGAYLVVSQFTLYADVRRGRRPGFTDAAPPELA
                     ERLVERFATRLRKA"
     gene            complement(354..>1933)
                     /locus_tag="VHK28_08460"
     CDS             complement(354..>1933)
                     /locus_tag="VHK28_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX2142286.1"
                     /translation="LVVSLVVTACGENGPLPFQPTPEPTPVQPREVAVLAVDRGSDAP
                     VPGAEVSVDGETSVTGQDGSAVVTALPGDRVEITAPGYDPGSGTVPDEGGLEVDLRPN
                     VVAGTVTDAAGEPVEGVRVFVDGSELYVRTDADGAYALPGVPESGTLIYKRAGYRLAE
                     LPIDEQMTKDVALEPFVARALYAPAAVFEGAGRLDELLALVERTEANALVIDVKETGG
                     WLYYETDLPEAVESGADRRPIFELEEILPALKERGIYLIARMVVMKDNTVGSSQPELA
                     VRNSATGEPWTDFGGGIWLDPFNPGVAEYIAAIAGDLADKGFDEVQLDYVRFFSDGDF
                     ATAETNLPNLQSFRLPAIRRLFRLVSERLATERAFLGADVFPISFIAPDDQGIGQRPE
                     VIMPYVDYFCPMVYPSHYGPGTFGIAQPNEAPYEIIDQTLEIMNEQSEGLPMVIRPWI
                     QDFGYGSFRAYNANDIRAEMKALTDNGAQGWMIWNARAIFTEAALGPPREGEDAGLGG
                     GVVSSPAASPVASPGSG"
BASE COUNT          288 a          699 c          663 g          283 t
ORIGIN      
        1 cgcctttcgc aggcgggtcg cgaagcgctc gaccagccgc tccgcgagct ccggcggtgc
       61 cgcatcggtg aagccgggac ggcggccgcg tcgcacgtcc gcgtacagcg tgaactggct
      121 gaccaccagg taggccccat cggcctcggc cagggccagg ttggtgcggc ccacatcatc
      181 gggaaagtag cgcaggacgg ccaggcgatc ggccatcgca tcggcagtgg catcactgtc
      241 gccggctccc acgccaacca gcaccaccgc gccccgcccg atgtccccca ccaccatccg
      301 gtcgaccgtg accctggcgc gcgttacgcg ctggacgacg agccgcaccg gcgctagcca
      361 gagccgggcg acgcgaccgg cgaggcggcg ggtgagctaa ccacgccgcc gccgagtccc
      421 gcatcctccc cctcgcgcgg cgggccgagg gcagcctcgg tgaagatcgc ccgcgcgttc
      481 cagatcatcc agccctgcgc gccgttgtcg gtcagcgcct tcatctcggc gcggatgtcg
      541 ttggcgttgt aggcgcggaa ggagccgtag ccgaagtcct ggatccaggg ccgaatcacc
      601 atcggcagcc cctcggactg ctcgttcatg atctccaggg tctggtcgat gatctcgtag
      661 ggcgcctcgt tgggctgcgc gatgccgaaa gtgcccggcc cgtagtggct gggatagacc
      721 atggggcaga agtagtcgac gtacggcatg atcacctccg gccgctggcc gatgccctgg
      781 tcatcggggg cgatgaagct gatggggaag acgtcggccc ccaggaacgc gcgctcggtg
      841 gccagacgct cgctcaccag gcggaagagg cgccggatgg cgggcagccg gaaggactgc
      901 aggttcggca ggttcgtctc ggcggtggcg aagtcgccgt cgctgaagaa gcggacgtag
      961 tccagctgga cctcgtcaaa gcccttgtcc gccaggtcgc cggcgatggc cgcgatgtac
     1021 tccgccacgc ccgggttgaa cggatcgagc cagatgccgc cgccgaagtc ggtccatggc
     1081 tcgccggtcg cgctgttgcg caccgccagc tcaggctgtg aggagccgac ggtgttgtcc
     1141 ttcatgacca ccatgcgcgc gatgaggtag atgccgcgct ccttcaacgc cggcaggatc
     1201 tcctccagct cgaagatcgg ccgccggtcg gcgccggact cgaccgcctc gggcagatcg
     1261 gtttcgtagt acagccagcc gcccgtctcc ttgacatcga tcaccagcgc gttcgcctcg
     1321 gtccgctcga cgagggcgag cagctcatcc agccgcccgg ccccctcgaa gacggctgcg
     1381 ggggcataca gcgcccgtgc cacgaacggc tccagggcca cgtccttggt catctgctcg
     1441 tcgataggca gctcggccag gcggtagccg gcccgcttgt agatcagggt cccgctctcg
     1501 gggaccccgg gtagcgcata ggcaccgtcc gcatcggtcc tgacgtacag ctcgctccca
     1561 tcgacgaaga cgcgcacgcc ctccaccggc tctccggccg cgtcggtgac cgtgccggcg
     1621 accacgttgg gccgcaggtc gacctccagc ccgccctcgt ccgggacggt cccggagccc
     1681 gggtcgtagc cgggtgccgt gatttcgacc cggtcgccgg gtaacgccgt gaccaccgcg
     1741 ctgccgtcct ggccggtgac cgacgtctcg ccatccacgc tcacctcggc gccgggcacc
     1801 ggtgcgtccg agccgcgatc gacggccagc acggcgacct cgcgcggctg gaccggggtc
     1861 ggctccggcg tcggctggaa gggaagcggc ccgttctcgc cgcaggcggt gaccaccagc
     1921 gagacgacca ggt
//
LOCUS       DATACA010000414         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2545170, whole genome shotgun sequence.
ACCESSION   DATACA010000414 DATACA010000000
VERSION     DATACA010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2545170"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VHK28_08465"
     CDS             <1..282
                     /locus_tag="VHK28_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142287.1"
                     /translation="MKAWLASRPPMTRAQKYDRLAFTFCKMGTVGLIAWLVTPPIFVL
                     AVALIAIYLYGRAVALGITRSQCVLRRPLLIMGFWAAVAAADATWLLVL"
     gene            294..1502
                     /locus_tag="VHK28_08470"
     CDS             294..1502
                     /locus_tag="VHK28_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142288.1"
                     /translation="MNELTRAQAADRAGVELAYLDRLIELEIIQPDDDERLTRGDVRR
                     ARMAETLEGAGIGLEALAAGIRSGRLSLAFMDTPSYQRFATLSDETFRQVSERTGIPL
                     DLLLVGREATGGALPSPDDRLREDEMVLVPFIETGLQSGLAARSVERQLRVLGDGMRR
                     LAETQGDAWRTDIMDPLLAQGASVAEIERMATAENSPDADLATDDAILAIWHAQQAHS
                     WGVNIIAGFEAILTDLGLLARPTSPPAICFLDVTGYTRLTQERGDEAAADLAASVNRL
                     VTRTSLDHGGRPVKWLGDGVMVYFREPGGGVMAALDMVEGVGAVGLPPAHVGLHAGPV
                     LFQEGDYFGRTVNIASRIADYARPGEVLVTQEVVDLSSEMGCRFSEIGPVELKGVEGT
                     VQLHAAHRAN"
     gene            complement(1524..1934)
                     /locus_tag="VHK28_08475"
     CDS             complement(1524..1934)
                     /locus_tag="VHK28_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142289.1"
                     /translation="MSDKPVTAAYYYRVRWGHHDEFLSLFRRNHYPILADQLASGRLL
                     DVRCYRPRFHSDGSADWTFMVTITYRDWAAMESHSEAEIAARLFPDQDRFRAEERRRF
                     ELVEAHWDVPLEDVDLADETDSQRQRKGLGFAPR"
     gene            1954..3021
                     /locus_tag="VHK28_08480"
     CDS             1954..3021
                     /locus_tag="VHK28_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142290.1"
                     /translation="MSRWLDWAGYAAAAWSAIYTVLNVYWWLGGAGFPFGPENDTGAE
                     LTILADARAASVAPIITVIGGAGALVGLAMSRSWRRGAVRAWLIGFGVTAAVILALLI
                     PDYRVLTVIAYTLVIVFGLPFGWPPEADLLGVLTGPILNQFWCIVGGLLWAGATLAYW
                     RRGRGACASCGRTDAAAGWSSPQHAPRWGRSATYVAVAIPVAYAATRWAWALGIPLGI
                     SEDFLRKGQEVGLWFIGAALATLAVAGALLTLGLTQRWGEVFPSWLPVIGGRNVPPML
                     AVVPAMLVAVVVTSAGLMFWRVTLRGEFPLGGLASLNLENAWAALLPELLWPLWGAAL
                     ATAAIAYYLRRRGHCARCGRG"
     gene            complement(3168..3611)
                     /locus_tag="VHK28_08485"
     CDS             complement(3168..3611)
                     /locus_tag="VHK28_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX2142291.1"
                     /translation="MLATVLLGIGLGGFFDGIVLHQVLQWHHLLTAHLPPTSVSNLEL
                     NTLADGLFHAFAWVVTAVGLLLLWSALRRPHQRWPSSLLAGGLLAGWGAFNLVEGIVD
                     HHILQIHHVRPGENELLWDLGFLAWGAVMLVIGLLLLRTSRVERT"
     gene            3717..>4171
                     /locus_tag="VHK28_08490"
     CDS             3717..>4171
                     /locus_tag="VHK28_08490"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142292.1"
                     /translation="MDPGGESGGERRSADEREAAASLSVGDRQKRLRRFWRSIPSPPR
                     CKLCHRPFGAPGGPLMKLMGLGPWPGNPKYCRGCFRALYRHRAGAEIECTLLFADVRE
                     STAMAEHMRPSDYRRLLDRFYATAFDILVRHDAFVDKFVGDEVIGIFVPA"
BASE COUNT          618 a         1358 c         1510 g          685 t
ORIGIN      
        1 atgaaggcct ggctggccag ccgcccgccg atgacgcggg cgcagaagta cgaccgcctg
       61 gcgttcacct tctgcaagat ggggacggtg ggtctgatcg cgtggctggt cacgcccccg
      121 atcttcgtcc tggccgtggc gctgatcgcc atctacctct acggccgggc ggtggccctg
      181 ggcatcacgc gctcgcaatg cgtcctgcgc cggccgctgc tgatcatggg cttctgggct
      241 gcggtggccg ccgccgacgc gacctggctg ttggtgttgt aggttccttc gtcatgaacg
      301 aactgaccag ggcgcaggca gcggatcggg ccggggtcga gctcgcgtac ctggatcgcc
      361 tgatcgagct cgagatcatc cagccggacg atgatgagcg cctgacccgc ggagacgtgc
      421 gccgcgcaag aatggcggag acgctggagg gcgccggcat cggcttggag gcactggcgg
      481 ccgggattcg gtccggacgg ctgtctctgg ccttcatgga cacgccgagc taccagcgct
      541 tcgcgacgct cagcgacgag acgtttcgac aggtgagcga gcggaccggg atcccgctcg
      601 acctgctgct ggtcggccgc gaggcgaccg ggggcgcatt gcccagccca gatgaccgcc
      661 tacgcgagga cgagatggtc ctagtgccgt tcatcgaaac agggctccaa agtggtctgg
      721 cggcgcgctc ggtggagcgc cagctccgcg tcctcggcga cggcatgcgc cgcctggcgg
      781 agacgcaggg cgacgcatgg cggaccgaca tcatggaccc gctgctcgcc cagggcgcat
      841 cggtggcgga gatagagcgc atggccaccg ccgagaattc gccagacgcc gacctggcca
      901 ccgacgatgc catcctggcg atctggcacg cccagcaggc gcactcgtgg ggcgtcaaca
      961 tcatcgccgg gttcgaggca atcctgaccg atctcggcct cctcgcccgg ccgaccagcc
     1021 cacccgccat ctgtttcctg gacgtgaccg ggtacaccag gctgacccag gagcgcggtg
     1081 acgaggcggc cgcagacctg gcggcatcgg tcaaccggct ggtcacccgc acgtctctcg
     1141 accacggcgg tcggccggtg aagtggctgg gagacggagt catggtctac ttccgcgagc
     1201 ccggcggcgg cgtcatggcc gcgctcgaca tggtcgaggg agtcggggcg gtgggccttc
     1261 cgccggcgca tgtcgggctg cacgccgggc cggtcctgtt ccaggagggc gactacttcg
     1321 gtcgcacggt gaacatcgcc tcgcgcatcg ccgactacgc ccgtccgggc gaggtgctgg
     1381 tgacccagga agttgtggac ctgtcgagcg agatgggctg tcggttctcg gagatcggcc
     1441 cggtggagct caagggcgtc gaagggacgg ttcaactgca cgcggcccat cgagccaact
     1501 gatggacgcg cggttataac gaatcagcga ggcgcgaaac ccagtccctt gcgctgacgc
     1561 tgcgaatcgg tctcatccgc caggtccaca tcctcgagcg gcacgtccca gtgggcttcc
     1621 accagctcga agcgacgacg ttcctccgcg cggaagcgat cctggtccgg gaacaggcgc
     1681 gcggcgatct ccgcctccga atggctctcc atggcggccc agtcgcggta ggtgatggtc
     1741 accatgaacg tccaatccgc cgagccatca ctatggaagc gcgggcggta gcagcggacg
     1801 tccagcaacc ggccgctggc aagctgatcg gccaggatgg ggtagtggtt gcggcggaag
     1861 agtgagagga actcgtcgtg gtggccccag cggacccggt agtagtaggc ggcggtcacc
     1921 ggcttgtcgc tcatggcccg ctagggtagt cgcatgagcc gatggctcga ctgggccggg
     1981 tacgcggcag ctgcctggtc ggcgatctat accgtcctga acgtctactg gtggctgggc
     2041 ggcgcgggat tccccttcgg acccgagaac gacacgggtg cggagctcac catcctggcc
     2101 gacgcccgcg ccgcctcggt cgcgccgatc atcacggtga tcggtggagc tggcgcgctg
     2161 gtcgggcttg cgatgagccg cagctggcgc cggggagcgg tgcgcgcgtg gctcatcggc
     2221 ttcggagtga ctgcggcggt catcctggcc ttgctgatcc cggactaccg ggtgctcacg
     2281 gtgatcgcct acacgttggt gatcgtcttc ggcctgccgt tcggctggcc tccggaggcg
     2341 gacctgctgg gtgtgctgac agggcccatc ctcaatcagt tctggtgcat cgtcggtgga
     2401 ctactatggg cgggcgccac gctggcctac tggcgtcgcg gtcggggtgc ctgcgcctcc
     2461 tgcggacgga ccgatgcagc cgccggttgg agcagtccgc agcacgcgcc gcgctgggga
     2521 cgatccgcga cctacgtggc agtcgcgatc ccggtggcgt atgcagccac ccgctgggca
     2581 tgggcactcg gtatcccgct tggcatcagc gaggacttcc tgcggaaggg acaggaggtc
     2641 ggcctgtggt tcatcggcgc cgcgttggcc acgctggcgg tcgcgggtgc cttgctgacc
     2701 ctgggcctca cgcagcgctg gggcgaagtg tttccgtcat ggctgccggt gatcggcggg
     2761 cgcaacgtgc cgcccatgct ggcagttgtg ccggccatgc tggtggcggt ggtggtcacc
     2821 agcgcggggc tgatgttctg gcgggtcacg ctgcgcgggg agttcccgct cggtgggctg
     2881 gcctcgctga acttggagaa cgcgtgggcg gcgctgctgc cggagctcct gtggccgctg
     2941 tggggagcgg cgctggcgac ggcggcaatc gcgtactacc tgcgtcgccg cggccactgt
     3001 gcacggtgcg ggcggggttg aggcgtatga cgaagggcgg cgccacgtcg acgccgccct
     3061 gccgcaaagt gttcagatga cctgaacact ttcagtcggc tccggccgcg ccctccgcga
     3121 gtccgttgag ccgctcgctc cacgcccgga cgctgagcct gccggctcta cgtgcgctct
     3181 acgcgacttg ttcgcaggag caggagaccg atgaccagca tcaccgcacc ccacgccagg
     3241 aaaccgaggt cccacagcag ctcgttctcg ccgggtcgca cgtggtgtat ctgcaggatg
     3301 tggtggtcga cgatgccttc caccaggttg aacgcccccc agccggccag cagcccgccg
     3361 gccagcagcg aggacggcca gcgttggtgg ggacgtcgca gcgcgctcca caggaggagc
     3421 aggcccacgg cggtgacgac ccaggcgaaa gcgtgaaaca gaccgtcggc gagcgtattg
     3481 agctcgaggt tcgagaccga ggtgggcggc aggtgggcgg tcaggaggtg atgccactgc
     3541 aggacctggt gcaggacgat tccatcgaag aagccgccca gaccgatgcc gagcaggacc
     3601 gtggcgagca tcggtcttgt tcggtcgtgt ccgcgctctg cttgggtcat gggatgcgcc
     3661 atcggcaaga agtgcgccga cgatctcgga cttgtccggg ggtggagaat ggcgccatgg
     3721 atcccggcgg ggagtcgggg ggcgagcggc ggtcggccga cgagcgcgag gcggcggcca
     3781 gcctgagcgt cggcgaccgg cagaagcgac tgcgacgatt ctggcgatcc attcccagcc
     3841 cgccgcgctg caagctctgc caccggccgt tcggcgcgcc gggcggcccg cttatgaagc
     3901 tgatgggcct tggcccgtgg cccggcaatc cgaagtactg ccgcggctgc ttccgcgccc
     3961 tctaccgcca ccgggctggc gcggagatcg agtgcaccct gctgttcgcc gatgtccgcg
     4021 agtcgaccgc aatggctgag cacatgcgcc ccagcgacta ccgtcggctg ttggaccgct
     4081 tctacgcgac cgccttcgac atcctcgttc ggcacgacgc cttcgtcgac aagttcgtcg
     4141 gcgatgaggt catcggcatc ttcgtaccgg c
//
LOCUS       DATACA010000415         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1582094, whole genome shotgun sequence.
ACCESSION   DATACA010000415 DATACA010000000
VERSION     DATACA010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582094"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..272)
                     /locus_tag="VHK28_08495"
                     /pseudo
     CDS             complement(1..272)
                     /locus_tag="VHK28_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            complement(304..1860)
                     /locus_tag="VHK28_08500"
     CDS             complement(304..1860)
                     /locus_tag="VHK28_08500"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HEX2142293.1"
                     /translation="MEAYDPSTAESAFHTSRTAFAALATRLADPAADRLADPAADRLD
                     HAALEEVVTTQGREVLRLLLQDHLDLRAVREREQQGRDRLPAPVDTHGTAHRAVETGH
                     TRQLATVVGRVTIRRAAHRARGAANLYPADAALNLPAGLHSFGVRRHCVQEAVRGSFD
                     TAIAAVTTVCGPVVAKRQARQLVRAAAADVAAFYTHATAAPPSTDATLLVLSFDSKGV
                     PMLPEALREETRKKAEATGGGPYRTRLASGQKSARKRMAAIAAVYDAAPAVRRPHDVI
                     QLAGAERAARRKGPKAQRKWLTASLARPARQVIAEAFDRACERDPAHRRTWVVLVDGD
                     NHQIELIQAEARRRGAPIHLVVDFVHVAEYVWRAAWCFFCNGEPEAERWVGIQLLGIL
                     SGKLEETLAEIRQRAQRAKLTGDQVRGITQCVRYLKAKKPFLDYTGALAAGWPIATGI
                     VEGACRHLIADRMDITGARWGLEDAEAVLTLRAVHSNGDFQQYWGFHMRQEYVRNHQA
                     RYQEGYHLTA"
     gene            complement(1951..2481)
                     /locus_tag="VHK28_08505"
     CDS             complement(1951..2481)
                     /locus_tag="VHK28_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142294.1"
                     /translation="MISSGSLREVIAVAGLLERLEQREEAARRRVEGLREQAARIAEQ
                     LADAEEALNRLVITRETVDEVLAEPELESAAVAVAVTASPAGVGPVPHRTAETAVAEL
                     PVSYRDVMEVLARVDEPLRAKDVAAAVGMGESSVKAEGMRSKLNRLADRGWIIKTDAG
                     GFTLDPVTRALVVGAM"
BASE COUNT          392 a          939 c          925 g          405 t
ORIGIN      
        1 cccgcgcggt agcggtggca caccggttcc agggcaccga agtcgccggt gcctttgaag
       61 tcgaacgcgt gcacctcggc ccgcgggtcg agggcggcga tgagcaggaa cagtcgcatg
      121 aggaacgtct tgcccatgcg cgggatggag ccgacgacca cggaggcgaa catgagcgtc
      181 acggcgacgt cgcgcatgcg ctggccgttg ccgaacacca ccggccggaa caggtccacc
      241 gtgcccgact tcgccagggg ccaggcgggc ttgggtgcgg gtccttcaca gggacttcgg
      301 ttatcaagct gtgaggtggt aaccctcctg gtagcgagcc tgatggttcc tcacatactc
      361 ctgtcgcatg tgaaaccccc agtactgctg gaaatcgccg ttgctgtgta ccgcgcggag
      421 ggtgagtacg gcctcggcat cctccaaccc ccatcgggca ccggtgatgt ccatgcggtc
      481 ggcgatcaga tggcggcacg cgccctccac aatgcccgtc gcgatcggcc agccggccgc
      541 gagtgccccg gtgtagtcga ggaacggctt cttggccttc agataccgga cgcactgggt
      601 gatgccgcgt acctggtcac cggtgagctt cgcgcgctgg gcccgctggc ggatctccgc
      661 cagggtctcc tccagtttcc cggacaagat gccgagcaac tggatgccga cccaccgctc
      721 ggcctccggc tcgccgttgc aaaaaaagca ccaggccgca cgccagacgt actccgcgac
      781 atgcacgaaa tcgaccacca gatggatggg tgcgccgcgc cgcctcgctt ccgcctgtat
      841 cagctcgatc tggtggttgt cgccgtcgac gagcaccacc caggtgcgcc ggtgggccgg
      901 gtcacgctcg caggcccggt cgaacgcctc ggcgatcacc tgccgggcgg gacgggccag
      961 cgaggcggtc agccacttgc gctgggcttt ggggcccttg cggcgcgcgg cgcgctcggc
     1021 gccggccagc tgtatgacgt cgtgggggcg ccggacagcc ggggcggcgt cgtagacggc
     1081 cgcgatggcc gccatccgct tgcgcgcgct cttctgcccg gacgcgagcc gggtacggta
     1141 cgggccaccg ccggtggctt ctgctttctt ccgggtttcc tcccgcaacg cctcggggag
     1201 catcggtacg cccttgctgt cgaaggagag caccagcagc gtcgcgtctg tgctgggcgg
     1261 tgcggccgtg gcgtgggtgt agaaggcggc gacgtcggct gcggcggccc gcaccagctg
     1321 ccgggcctgt cgtttggcca ccaccggacc gcacacggtg gtgacggcgg cgatggcggt
     1381 gtcgaacgag ccgcgcaccg cctcctgcac gcagtgcctg cgcacgccga aggagtgcag
     1441 cccggccggg aggttcagcg cggcgtcggc cgggtagagg ttggcggcgc cgcgggcgcg
     1501 gtgggcggca cggcgtatcg tcacgcggcc gacgacggtg gccagctgcc gggtgtgacc
     1561 ggtctcgacg gcgcggtgcg cggtgccatg cgtatcgacc ggcgcgggca ggcggtcgcg
     1621 gccttgctgt tccctctcgc gtaccgcgcg caggtccagg tggtcttgta gcagcagccg
     1681 cagtacctcc cggccctggg tggtcacgac ttcctccagc gcggcgtggt cgagccggtc
     1741 ggcggctggg tcggcaagcc ggtcggcggc tgggtcggcg agccgggtgg caagggcggc
     1801 gaatgccgtg cgggaggtgt gaaaggcgga ttcggccgtc gaagggtcat aggcttccat
     1861 ggcaggggct ctttctccgc gtcgctggaa ctcatcgctt tcgacgctag ggggaagagc
     1921 ctccgtccac ggcggttgcc gcgatccgcc ctacatcgcg ccgacgacga gggcgcgggt
     1981 gaccggatcg agggtgaagc ccccagcgtc ggtcttgatg atccagccgc ggtcggcgag
     2041 ccggttgagc ttcgaacgca tcccctccgc cttcacagag gactcgccca tcccgacagc
     2101 cgccgccacg tccttggcgc gcaacggctc atcgacgcgg gcgagcacct ccatcacgtc
     2161 ccggtaggaa accggcagtt ccgccaccgc cgtctcagcc gtccggtgcg gcaccggacc
     2221 cacaccggcc ggcgaggccg tcaccgccac agcaacggcc gccgactcca gctccggctc
     2281 ggcaagcacc tcgtcaacgg tctcacgggt gatgaccagc cggttcagcg cctcctcggc
     2341 atcggccagc tgctcggcga tgcgggcggc ctgctcgcgc agcccctcca cacgccgccg
     2401 ggcggcctcc tcccgctgtt caagccgctc cagcaaaccc gccaccgcga tcacctcccg
     2461 aagcgagcca gaggagatca ttcctacccg agaaggacca gggctcgacc tcccgcttca
     2521 acaggtccct ctgaaggacc cgcacccatc ggcggaggag agtcaaaccc ccgttggaca
     2581 gctacaacct gagcctgctc gccaagcagg ccggccgctg tcctctccgc ggggcacatc
     2641 tcctcagagc cgatcagcca c
//
LOCUS       DATACA010000416         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1020253, whole genome shotgun sequence.
ACCESSION   DATACA010000416 DATACA010000000
VERSION     DATACA010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020253"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VHK28_08510"
     CDS             <1..959
                     /locus_tag="VHK28_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX2142295.1"
                     /translation="FLGGPPLVKAATGEEVSAEELGGAEVHTTQSGVADHYALDDEHA
                     VAIGRQIVAGLHRRKPEPPWERAEPREPSVDPEGLYGVIPADNRQSYDVREVIARLVD
                     DSDFHEFKERYGETLVCGFARIDGYPVGILANNGILFSSSSLKGAHFIELASQRRIPL
                     VFLQNITGFMVGREYEAGGIARDGAKLVTAVATTAVPKFTVIIGGSFGAGNYGMAGRA
                     YSPRQLWMWPNARISVMGGQQAARVLSTVRGGFQTDAERDAFEAPILEKYEREGSPYY
                     STARLWDDGVIDPLDTRRVLALGLSAALNAPIRETRFGIFRM"
     gene            complement(973..1734)
                     /locus_tag="VHK28_08515"
     CDS             complement(973..1734)
                     /locus_tag="VHK28_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2142296.1"
                     /translation="MPQGIDHIVLAAPDLDAAAGELGEALGIAFTAGGQHPGAGTRNR
                     IAFLGDAYLELIGVDDERLAEQRPIGAAAVRALQDRGGLATYSLVDDHLETTVAELQA
                     NGSSIGPVDAGSRQRPDGERVEWWTATFERLGPDRPPFLIRHSLAGAEWGAEALEQRR
                     AYRHPIGSTVRLVRLDIATADPPALAAEYHAELGLEFWAVADLAVTSVGQHTIRLRPA
                     GEMQFPAAVLLGADVPAARSVEALGVRFLIEPVAA"
     gene            1773..2579
                     /locus_tag="VHK28_08520"
     CDS             1773..2579
                     /locus_tag="VHK28_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX2142297.1"
                     /translation="MTLERPAPGSHDRSTPTGTLEESAANLFEAMLAARDAEDRGELA
                     GPALEQFYRSLMTGTLLLPVPPEHGDEAKEALEKAVTDDEEVEISVLLAADADGQAIS
                     VCFASVAALSAWAPKGTATLPIPARIAVANMAASGVPAIMDPAGPIPYRFEPDELAAL
                     AAGRMPGSERPLFDTAPRRSLRLRLPGADAVALESSLARELAGTDVDAAWLVESESDG
                     QRRLMLGLVGREGASATVDVPDGTEVVWLEEPLLGSVKAVAEPFYRRGRR"
     gene            2579..3379
                     /locus_tag="VHK28_08525"
     CDS             2579..3379
                     /locus_tag="VHK28_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2142298.1"
                     /translation="MASLRVERDGSVARVTITRPEVRNAFNAALIGELHEAFDALARE
                     PAEALRAVVLAGEGPIFSAGADIAWQRAALQLSLEENEADAARLQAMLRAIDECPAPV
                     VARVQGAALGGGMALCAVSDVVVATADARFGFTEAKLGILPAVISPFVLPRIGQGHAR
                     AVYLTAERFDAQRALRIGLVHEVVADEAALEARVRELVVELRTSGPTAVRRAKALIRD
                     RRGMSPHEFAAHSVGIAARQRVSDEGQEGLAAFLDKRRASWIEPPGDD"
     gene            complement(3437..>3993)
                     /locus_tag="VHK28_08530"
     CDS             complement(3437..>3993)
                     /locus_tag="VHK28_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017732645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX2142299.1"
                     /translation="FEGPDGPGIRPTPYSRDMAVNGSTYQTVITDAGGTLSIPHGVGY
                     VWATMLWEMYGNLVDEHSFSGDIYADSTAGGNNLALQLVIDGLKFQPCSPGFVQGRDA
                     ILDADMALTGGDNQCLIWEAFAKRGLGVSAEQRSSKKANDGTAAFDLPAECGSGAGVV
                     VPLAPAILLRELWRLRPRRLIVRD"
BASE COUNT          552 a         1404 c         1480 g          557 t
ORIGIN      
        1 tcttcctggg aggcccgccg ctggtgaagg ccgccaccgg cgaggaggtg tcggccgagg
       61 agctgggcgg tgccgaggtg cacacgacgc aatcaggggt ggccgaccac tacgcgctgg
      121 acgacgagca cgcggtcgcc atcggtcgcc agatcgtggc cggtctgcac cgcaggaagc
      181 cggagccgcc gtgggagcga gcggaaccgc gggagccatc ggtcgacccg gagggcctgt
      241 acggggtgat cccggccgac aaccggcaga gctacgacgt gcgagaggtg atcgcgcggc
      301 tggtggacga ttccgacttc cacgagttca aggagcggta cggggagacg ctggtctgcg
      361 gcttcgcgcg catcgacggc tacccggtcg gcatcctggc caacaacggg atcctgtttt
      421 ccagctccag cctgaaggga gcgcacttca tcgagctggc cagccagcgg cgcatcccgc
      481 tcgtcttcct gcagaacatc accggcttca tggtggggcg cgagtacgag gcggggggca
      541 tcgccaggga cggcgccaag ctggtgaccg cggtggccac cacggcggtg cccaagttca
      601 cggtgatcat cggcggcagc tttggcgcgg ggaactacgg catggccggg cgcgcctact
      661 cacctcgcca gctgtggatg tggccgaacg cccgcatcag cgtcatgggc gggcagcagg
      721 ctgcccgcgt gctgtccacc gtccgcggcg gcttccagac cgatgcggag cgcgacgcct
      781 tcgaggcccc gatcctggaa aagtacgagc gcgagggttc cccgtactac tccaccgcgc
      841 ggctctggga cgacggcgtg atcgacccgc tggacacgcg tcgggtgctg gcgctcgggc
      901 tttcggcggc gctgaacgcg cccatccggg agacgcgctt cggcatcttc cgcatgtgat
      961 cgccgcggca ggctaggcgg ccaccggctc gatcaggaag cgcacgccga gcgcctcgac
     1021 gctgcgcgcc gcgggcacgt cggcgcccag caggacggcc gccgggaact gcatctcacc
     1081 ggctggacgc aggcggatgg tgtgctgccc gacgctggtc accgccaggt cggccaccgc
     1141 ccagaactcc aggccgagct cggcgtggta ctcggcggcc agcgccggcg gatcggcggt
     1201 ggcgatgtcg aggcgcacca gtcgcaccgt ggagccgatc gggtggcggt aggcgcgccg
     1261 ctgctcgagc gcctccgcgc cccactcggc gccggccagg gagtggcgga tgaggaacgg
     1321 cggccggtcg gggccgagcc gctcgaaggt ggccgtccac cactccacgc gctctccgtc
     1381 cggccgctgg cgggagccgg catcgacggg accgatgctg ctgccgttcg cctgcagctc
     1441 ggccaccgtc gtctccaggt gatcgtccac gagcgagtag gtcgccaggc cgccgcggtc
     1501 ctgcagggcg cgcacggcgg ccgcgccgat cgggcgctgc tcggccagcc gctcgtcgtc
     1561 cacgccgatc agctcgaggt aggcgtcgcc caagaaggcg atccggttgc gggtgcccgc
     1621 ccccgggtgc tggccgccgg cggtgaaggc gatgcccagc gcctcgccca gctcgccggc
     1681 cgccgcgtcc aggtccggcg cggcgaggac gatgtgatcg atcccctgcg gcatgccgag
     1741 atcgtaccgc ccccgatggt aggctgcgat cgatgaccct ggaacgtccc gcccccggct
     1801 cgcacgatcg aagcacgcca accggaacgc tggaggagtc ggccgccaac ctgttcgagg
     1861 ccatgctggc cgcccgtgac gcggaggatc gcggcgagct ggccggcccg gcgctcgagc
     1921 agttctaccg ctcgctgatg accggcacgc tgctgctgcc tgttccgccc gagcacggcg
     1981 acgaggccaa ggaggcgctg gagaaggcgg tcaccgacga cgaggaggtc gagatcagcg
     2041 tgctgctggc ggccgacgcc gacgggcagg ccatcagcgt ctgcttcgcc tcggtggccg
     2101 ccctgtcggc gtgggcgccc aaagggactg ccaccctgcc catccccgcc cgcatcgcgg
     2161 tggccaacat ggccgcctcc ggcgtgccgg ccatcatgga tcccgccggc ccgatcccct
     2221 accgcttcga gcccgatgag ctggcggctc tggccgccgg ccgcatgccg ggcagcgagc
     2281 gaccgctgtt cgataccgct ccgcgccgct ccctgcggct gcggctgccg ggtgccgacg
     2341 ccgtcgccct cgagtcgtca ctggcgcgcg agctggccgg gaccgatgtg gacgccgcct
     2401 ggctggtcga atcggagagc gacgggcagc gccgcctgat gctggggctg gtgggtcgcg
     2461 agggagcatc ggccaccgtg gacgtgccgg acgggaccga ggtggtgtgg ctcgaggagc
     2521 cgctgctcgg ctcggtcaag gccgtcgccg agcccttcta ccgtcgaggg cgtcgctgat
     2581 ggcgtccctg cgcgtcgagc gtgacggatc cgtcgcgcgg gtgaccatca cccgtccgga
     2641 ggtgcgcaac gcgttcaacg ccgccctcat cggggagctg cacgaggcgt tcgacgccct
     2701 ggcccgcgag cccgccgagg cgctgcgggc cgtggtgctg gccggggagg gaccgatctt
     2761 ctcggccggt gcggacatcg cctggcagcg cgccgccctg cagctctccc tggaggagaa
     2821 cgaggccgac gccgcccgcc tgcaggccat gctgcgcgcc atcgacgagt gtcccgcgcc
     2881 ggtcgtcgcg cgggtccagg gagctgcgct gggcggcggc atggcgctgt gcgccgtgtc
     2941 ggacgtggtc gtggccaccg ccgatgcccg cttcgggttc accgaggcca agctcggcat
     3001 cctgccggcg gtgatcagcc ccttcgtgct gccgcgcatc gggcaaggtc atgcccgggc
     3061 cgtgtacctc accgcggagc ggttcgatgc acagcgcgcg ctgcgcatcg ggcttgtcca
     3121 cgaggtggtg gcggacgagg ccgcgctcga ggctcgcgtg cgagagctag tggttgagct
     3181 gcgcacgtcg gggcccaccg ccgtccgccg ggccaaggcg ctgatccgcg acaggcgggg
     3241 catgtcgccc cacgagttcg cggcacacag cgtgggcatc gccgcgcggc agcgggtgtc
     3301 ggacgaaggc caggaggggc tggccgcctt cctggacaag cgccgcgcca gctggatcga
     3361 gccaccgggc gacgactaga tacggcgagg cggccgccgg agcggccgcc tcgtcatcgg
     3421 tccacctgga gggtggtcag tccctgacga tcagccttcg aggccggagg cgccagagct
     3481 ctcgcagcag gatggcgggt gccagcggca ccaccacacc cgccccgctg ccgcactcgg
     3541 ccggcaggtc gaaggccgcg gtgccgtcgt tcgccttctt cgacgaacgc tgctcggcgc
     3601 tgacgcccag accgcgcttg gcgaatgcct cccagatcag gcactggttg tcaccaccgg
     3661 tgagggccat gtccgcatcc aggatggcgt cgcgaccctg cacgaagccg gggctgcacg
     3721 gctggaactt caggccgtcg atcaccagct gcagggcgag gttgttgccg ccggcagtcg
     3781 agtcggcgta gatgtcaccg ctgaaggagt gctcgtcgac caggttgccg tacatctccc
     3841 acagcatggt ggcccacacg taccccaccc cgtgcgggat ggagagcgtg ccaccggcat
     3901 cggtgatgac ggtctggtag gtggagccgt tgactgccat gtcacggctg tagggcgtgg
     3961 gccggatgcc cggtccgtcc gggccctcga aca
//
LOCUS       DATACA010000417         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2224748, whole genome shotgun sequence.
ACCESSION   DATACA010000417 DATACA010000000
VERSION     DATACA010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224748"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VHK28_08535"
     CDS             <1..369
                     /locus_tag="VHK28_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142300.1"
                     /translation="GGTGIALSAIVHVVLQQLAITRFGYFDADFVGWWTGGMFAVLIG
                     LATGAFGVFVAPRGAAAWPMMAVLLGCAGVLLVLLANLSGLGDGIAPESWPLAIWLGI
                     SGLYAAGLAISATVRVRAAR"
     gene            complement(538..>1760)
                     /locus_tag="VHK28_08540"
     CDS             complement(538..>1760)
                     /locus_tag="VHK28_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2142301.1"
                     /translation="VYRRDLKDMPAQDEVHEAIEEGARVIFQASPVRILTDDRNRVTG
                     VEFQRMKLGEPDDKGRRRPEPMPGTEFVVECDTVLGAIGQGPDLAWVESEPEEVRAQL
                     EVSRRGTLVSEEDIFRTGLPRVFASGDVRTGATTVVEAIGEGRRASYAIDYWLRGHDL
                     DDPQVRRIVSEPTPNFLTIVPFTDDVKEPRAVMGKLGAEERRDNFGEYEYGYTHDQAM
                     GEAARCLQCTCEAIGHCDLREAAIEYETTLNMAIDERSIQDNPYVGGNHGYGRDETHS
                     FILRDYSRCIDCGRCAQVCKDIVGAGCYDFIGKGFDSLVTTADFVSLNETPCVSCGRC
                     AETCPVGALMPRPRTLETYQLDVSRCIFCGICADACPYDALRCGPEFEFSDYQRDLPM
                     LDILEMSDRERPTR"
BASE COUNT          252 a          589 c          623 g          296 t
ORIGIN      
        1 ggcggaaccg ggattgcgct gtcggcgatc gttcacgtcg tcctgcagca gttggcgatc
       61 acgcgcttcg ggtacttcga cgccgacttc gttggatggt ggactggcgg catgttcgcg
      121 gtgctcatcg gcctggcgac cggcgccttc ggcgtcttcg tcgcaccacg gggcgccgcc
      181 gcctggccga tgatggccgt cctgctcggt tgtgccggcg tcctattggt gctcctggca
      241 aatctctccg gcctggggga cgggatcgct cccgagagct ggccgctggc aatctggctg
      301 gggatcagcg gtctgtacgc agctggcctg gccatctccg cgactgtccg cgtgcgagcc
      361 gcccggtagg gcaagccaag agcatcaacg gcgagacggc cttcacgctc tgtccgcctg
      421 aaccgcaggc tatttcgtgg atgaggcgtg ggcgcgcggc cgcaggctgc ggcaattgcc
      481 agccaaggag tcgtgagccc acgccacaac gaccgacaga catcggtcca ggatgaacta
      541 gcgggtggga cgctcccggt cggacatctc caggatgtcg agcatgggca ggtcgcgctg
      601 gtagtcgctg aactcgaact ccgggccgca gcgcagggcg tcgtacgggc aggcatccgc
      661 acagatgccg cagaagatgc agcgcgacac gtccagctgg taggtctcca gcgtccgtgg
      721 gcgcggcatc agcgcgccga ccgggcaggt ctcggcgcag cggccgcatg acacgcacgg
      781 cgtctcgttg aggctcacga agtcggcggt ggtgacgagc gaatcgaagc ccttgccgat
      841 gaagtcgtag cagccggcgc ccacgatgtc cttgcagacc tgcgcgcagc ggccgcagtc
      901 gatgcaacgc gagtagtcgc gcaggatgaa ggagtgggtc tcgtcgcggc cgtagccgtg
      961 gttgccgccg acatacgggt tgtcctggat cgagcgctcg tcgatggcca tgttgagcgt
     1021 cgtctcgtac tcgatggccg cctcgcgcag gtcgcagtgg ccgatcgcct cgcaggtgca
     1081 ctgcaggcag cgcgcggcct cgcccatggc ctggtcgtgg gtgtagccgt actcgtactc
     1141 gccgaagttg tcgcgccgct cctctgcgcc gagcttgccc atgaccgcgc gaggctcctt
     1201 gacgtcgtcg gtgaagggca cgatggtcag gaagttgggc gtcggctcgg acacgatgcg
     1261 gcgcacctgg ggatcgtcca ggtcgtggcc gcgcagccag tagtcgatgg cgtacgaggc
     1321 gcgccgtccc tcaccgatgg cctcgaccac ggtggtggcg ccggtccgca cgtcgcccga
     1381 ggcgaacacg cggggcagtc cggtgcggaa gatgtcctcc tcggagacca gcgtgccgcg
     1441 gcggctcacc tccagctgcg cgcggacctc ctccggctcc gactcgaccc aggccaggtc
     1501 cggcccctga ccgatggcgc ccagcaccgt gtcgcactcc accacgaact ccgtgcctgg
     1561 catcggctcc gggcggcggc ggcccttgtc gtccggctcg cccagcttca tgcgctggaa
     1621 ctcgacgccg gtgacccggt tgcggtcatc ggtgaggatg cgcaccggcg aggcctggaa
     1681 gatgacccgc gccccctcct cgatggcctc gtgcacctcg tcctgggcgg gcatgtcctt
     1741 gaggtcgcgg cggtagacca
//
LOCUS       DATACA010000418        13028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2706569, whole genome shotgun sequence.
ACCESSION   DATACA010000418 DATACA010000000
VERSION     DATACA010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 13028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13028
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2706569"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VHK28_08545"
     CDS             <1..478
                     /locus_tag="VHK28_08545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX2142302.1"
                     /translation="AFLAILLLGGPLQEEFGWRGYALPRLVARFRPPAATLVLAVVWT
                     GWHLPFWFMHGSGMAGTPLPVYFVYMLGLTAVMTWLYVRTAGSVLAAILMHATANWIS
                     NVLPTHTAETVASNAYLFQAVGYLLIGALLLWRTSHASARSPRGAHAVGSSWRIAD"
     gene            519..932
                     /locus_tag="VHK28_08550"
     CDS             519..932
                     /locus_tag="VHK28_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013667591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX2142303.1"
                     /translation="MTTIEQPFDTRIAAASPWRLNSLILRLEALAALGAGIALWLAND
                     GSLLWLLPALLVPDLSMAGYVANSRVGAVTYNLIHNWVLAPALLGLGWWMGSDVLLLA
                     GSVVLAHVGMDRAMGYGLKLPTDFRDTHLGRIGAK"
     gene            complement(1149..1586)
                     /locus_tag="VHK28_08555"
     CDS             complement(1149..1586)
                     /locus_tag="VHK28_08555"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX2142304.1"
                     /translation="MDAETFEKLVSRFGPDIAELARAARRLVVEFMPGVYEIVWPGMQ
                     TVGYGTGPKKMTEHFAWLSPYGGHVVLGFYYGAELPDPEGLLEGTGKRMRHLKLRSAD
                     DLRRPGVSALLEHASRHRVPPLRDDPRAAADLEKARKSALSGA"
     gene            complement(1878..3080)
                     /locus_tag="VHK28_08560"
     CDS             complement(1878..3080)
                     /locus_tag="VHK28_08560"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142305.1"
                     /translation="MQLPKSRAAAESGATRQELDRFIEAGIIRPADDGTLTWAELQRV
                     RAVKQFLDAGVGWEHMRRAVDEGLITFDYADAFYLEPAGPSGRTYREYRDSLGPNAQH
                     LSRVYDTLGLAEPPPDRPMREDEEGCLAELVDLWMRVGEQEALLRATRLLGDHMRAMV
                     EGWMALWTEHMRYVGVDAESLQQRADFTLVLGRGLTGLLPRIMVWAEQRYLERAMTAV
                     GVEQMEEVMAAHGIAPAPPEHPPAVLFVDLAGFSRLTEQHGDEAAVLYGTRLRDVAER
                     AARGHRGKLVKLLGDGAMLHFEAPQDAVGAGRQLLSTEGWDADLPDPHIGAHAGPVIE
                     RDGDLFGTTVNIAARLSSSAGAGEFIVSSDVVAGLTQPDEIALRPMGELILKNIDRSI
                     SAFSVVAR"
     gene            complement(3245..3321)
                     /locus_tag="VHK28_08565"
     tRNA            complement(3245..3321)
                     /locus_tag="VHK28_08565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3285..3287),aa:Glu,seq:ctc)
     gene            complement(3439..4305)
                     /locus_tag="VHK28_08570"
     CDS             complement(3439..4305)
                     /locus_tag="VHK28_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX2142306.1"
                     /translation="MVQAIKRDEVYIDPEGIYVETHQPERRSRKPPLLLVHGALTGSW
                     MWSSFATFFAERGWEAHAMNMRGHYTSDVADLGSVSMRDYADDVGVAIRHLGRPPVVI
                     GWGIGALAAMLYAERNPVLGLVLLAPSPPAAALPGRPSDMELKDVPPVYDARWWGWIV
                     SSERAREMMPDMTDAELEKVQEMLDGARESGSARRERMLGVSVESVRIEAPALVIAAG
                     RDDVIHPTEARRTADLLGAAYEYFPSASHFGLVMGSETWPQVAGSVLGWLEERRHAML
                     AVMNASLAGAAR"
     gene            complement(4314..5660)
                     /locus_tag="VHK28_08575"
     CDS             complement(4314..5660)
                     /locus_tag="VHK28_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEX2142307.1"
                     /translation="MPLVRPLPGVSYALDRYAPQMVPERVRLLDEPPTSAGRGVADVT
                     DLVCPPYDVISPAQQRELLARDERNAVRLELSAEPDPHAAAASTLEAWLADGTLRRRA
                     EPSVYYYAHATLDRPDEPTVQGVLARVLLEPFGDQVRAHEHTMPGPKADRLALLHATR
                     TQLSPILAVYFDRSARYPHIMSRAWADEWRARDGDRLLHALAAVEPDERLLNYLSRQT
                     LYVADGHHRYETALAYQAEVRADPRWTDAPAGALAADWIMVVMVNAEREELSILPTHR
                     LIRQAEPDALRSLVTDPGPMWHAEPVAPDELATRLEELRAAEEPIFGLVLPDGEGWLM
                     VGDPEVVADRMRRERVSTAVARLDLAVLHAAVLADRLGIDAAAVAAGGPLAYTRSEAD
                     ARASVAAGEAFAAILVRPTPLEQMARVATVGDVLPQKSTYFYPKLLTGMVFNPLED"
     gene            complement(5675..7594)
                     /locus_tag="VHK28_08580"
     CDS             complement(5675..7594)
                     /locus_tag="VHK28_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2142308.1"
                     /translation="MDATRARPTDLEEHLERRLRLQERQLAVARLAIVGAALVAVVIF
                     REALAAFPWLLGLGLAAVAFSLATMLLVGRFPAREVGVVATAADMVVVTLAIYVEPRA
                     IDAFVFYFPVVLGVALRFGFGAALWSAVVVSFVYGSVVILAAQPGTPTRELLGVRIGY
                     LLGLGLTAGLFARAVMGRAEENARLQLRLAEEERERARAREVELLSQLAREFGSSLDQ
                     HTTADAVIRAAAPLLGELSWLLLVETDHGEGQQPSLTLAAADGRDAQRVEQLVAHVGK
                     RSLRMGEGLAGAAAATVLPVVVGGQNPVPVHPGDPDGVAALGLRSLVAAPIVSRGQVW
                     GVLVTASAGGPTMGEGETRLAMAIAERAGPALENAALWADLQEQVAREQRAQRVKDDF
                     LSIVSHELRTPLTSIQGYSQLLEARMRDSATAKELSQMRVIRSQVARMRRLVDDLLDV
                     SRIDRRGGVSVEPASIDLADEIREAVARTEREHTDRRVTLHVPESLPVEADRDRIGQV
                     LTNLLDNAIKYSPDGGPVEVRAEGHGDWLEVTVADTGVGIDPGDTELVFERFYQVAEA
                     ETSGGGRRFGGLGLGLYITRAILRSHGGDITAEPNRAAGRGTVMRVRLPRRARVPEAL
                     RPVTDEPPPFVARRR"
     gene            7740..10148
                     /locus_tag="VHK28_08585"
     CDS             7740..10148
                     /locus_tag="VHK28_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142309.1"
                     /translation="MTRRPTAALAMLATLLAVTVLVASPGYGLAADPVAMEARAQVAG
                     RFEAGGWAAISVRLANDGEPVTGYVAADGEDGTVRRLVELPAGSRKEVTLYLRPESFA
                     RTVAVRFETSDGEPVASAEADVAVLERTSATVAVVGDVTGILRPQLMARDTGIPEPVA
                     MTPADITERPEPMRGLETILWAGDSSTLTEAQRRSLERWIAAGGQLVVVGGPDWQART
                     AAFEAFLPVEDLRSVDEVGLAPLATWVAHELPPEGVSASVSLGQLRSDAVALVGTGDD
                     VLLASVVRGAGRVSWLGVDLATEAFRAWPGAPGLWQRLLPNDRAAEQFGAVPPLEEEV
                     AGLMVQALSNLPALDVPPAELLLAVLVGYILLIGPLSYLALRRLDRRELAWVTAPILV
                     LVFSSVSYGIGASMKGSDVILNEVAVVRTTTDGTAASVSSFAGVFSPTRRAYDITVAG
                     EALFSRLVNPFDGTQRGGGLATEQGHPSRLRGLSVSVFGLEGIRADTLIPYTPALSVT
                     WSFTSEGIRGSATNTGEEPMEDVALVTEGGGAMVGTLDAGQTKEFALSMRGANQRSAS
                     EQIYGQVSGVSGSDTQRRIALRRQVIDTLVGYGGWTGRLGGGLSGIDRGPYVIGWRQG
                     AAPVAMEVDGERAQHYVQAVEVVSGRPRLGPGAVTILPAHISTDVLELIGDANEAEPG
                     YVVVGEGEAVFGMTLPLEATGLAASSITLLAGSDPSMVVSDQPNFGAALPPGYTLSVW
                     DNLSSSWEEVGDLAARSRFELEQPGGLIDASGRIVVKITGTEVDPNFGASGVFVGAVV
                     EGVI"
     gene            10154..11122
                     /locus_tag="VHK28_08590"
     CDS             10154..11122
                     /locus_tag="VHK28_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142310.1"
                     /translation="MRPVIETRGLVKRYDGETAVAGVDLVVQPGEIYGLVGPNGAGKT
                     TTMRMLATLLAPTAGQAVVCGVDVMDDPVEVRRRIGYMPDFWGVYDDLRVWEYLDFFA
                     RCYGVPTSRRASVIGELLEIVDLGDKRDAYVESLSRGMRQRLCLAHTLVHDPELLILD
                     EPASGLDPRARVEMREILRALSGMGKTILVSSHILPELGELCTAVGIIDHGRLLRSGR
                     IADIERSLQATAQLRIDLLSNLEQAVAFLGTDPRVSSVLEIEGGIGTARVEVSFDGGP
                     EEQSALLQAMIEDGHRVAGFGQATSGLEEIFLRVTGSVAGDEETAA"
     gene            11119..12318
                     /locus_tag="VHK28_08595"
     CDS             11119..12318
                     /locus_tag="VHK28_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142311.1"
                     /translation="MTAASTATGGTRRGDLRELASSAATIMVKELRSRFRGRRAFLVL
                     TLYLAVLAALAYGMYTVTGPAARNAAQVGAQFGDPSAINASARVGQGIFSILSIFQLL
                     LIGFIAPAFTAGSISLEREKQTLDLLIATPIRPAGLVIGKLLSALAFVVLMIVAAIPI
                     SALVLMYGGASIDDILRQQAVLFASAIGFGVIGLFWSALTKRTQAATVLTYSTLLVVT
                     IGTLLVWRFWTDVATAAPDNTFGEVRTAPEELLYINPAVAMIEIVANTEIEYGDFSEA
                     YDALRGGAGNVVCDGDVCFPASDGRFVPLAPPGADFDEEGRVPMGIDFAVPPDRLTVN
                     DGVFNHFWPRITATYAALSVLLTLAAMRLVQPAGTRLRFAPRRRSSDPDTAVPLEAEG
                     SIEEMRP"
     gene            12315..>13028
                     /locus_tag="VHK28_08600"
     CDS             12315..>13028
                     /locus_tag="VHK28_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142312.1"
                     /translation="MTDRQLADALRARLGDDMVARLRKLRRRLWLRRAVRIGALTTIA
                     VLGLLAAVQLVARSMPLEAAPWLMAAAGGFGLLAWLAVSWSRRPSLLETAHRADEELA
                     LRERLGTAAELVLRPDDRDVPADVVTLEGRQLADARARLAEVELNRAFRPRLSRRGLA
                     AGGIALALLLVLTVWPNPQDGILRDRQAARDASRDVAERIDDVADEAERRGAETDDPR
                     RDELVERLRELARKLRESGD"
BASE COUNT         1882 a         4614 c         4537 g         1995 t
ORIGIN      
        1 ggcgtttctc gccatcctgc tcctcggcgg cccgcttcag gaggagttcg gctggcgcgg
       61 atatgccctc ccgcgcctgg tggcgcgctt ccggccgccg gcggcgacac tcgtgctcgc
      121 cgtcgtctgg actggatggc acctgccatt ctggttcatg cacggctcgg gcatggccgg
      181 cactcccctg ccggtctact tcgtctacat gctcgggctc acggccgtga tgacctggct
      241 ctacgtccgg accgcgggaa gcgttctggc cgccatcctc atgcacgcca ccgccaactg
      301 gatctccaac gtgctgccga cccacaccgc ggagaccgtc gcttcaaacg cctacctgtt
      361 tcaagccgtc ggctacctgc tgatcggtgc actgctgctg tggcggacca gccacgccag
      421 cgcacggtcg ccccgaggcg ctcatgccgt cggcagcagc tggcggatcg ctgattagaa
      481 cctgccccat gtatcggggc tggcatacgg agagattgat gaccacgatt gaacagccgt
      541 tcgacacacg catcgcggca gcgtcgccat ggcgtcttaa tagcttgatc cttcgcctcg
      601 aggcgctggc cgctctcggt gccgggatcg cgctctggct cgcgaacgat ggctcgctgc
      661 tgtggctgct tccggcgctc ctcgtgcctg acctgtcgat ggccggctac gtcgcgaaca
      721 gccgcgtggg cgcagtgacg tacaacctga tccacaattg ggtgctggcg ccggcccttc
      781 tcggattggg ctggtggatg ggaagcgatg tgctgctcct cgccggctcc gtggtgctgg
      841 cacacgtcgg gatggaccgg gccatgggct acggtttgaa gctgccgacg gacttccgag
      901 atacccacct cggcagaatc ggtgctaagt aacggtgcgc ggctcggtca ctgacgaata
      961 ggccgggagg aggccacgat aacggtgacg acggcctcga tccaggttcg ccgttcctcg
     1021 aatgacattt cgaccgatcg gtgtgacgct cacggtaggc cggtccgcgc gcgcggcaac
     1081 gatcgccggc gcaaacctgt cagcgacacg accgccggct ggctgccgat ccctggctga
     1141 tcgtgaccct acgctccgga tagagcggac ttccgagcct tttcgaggtc tgcagcggcc
     1201 cgcggatcat ctcggagagg cgggacgcga tgacggctcg cgtgctccag cagcgcgcta
     1261 acccctgggc gccgcaggtc atcggcactg cgcagcttca ggtggcgcat gcgtttgccg
     1321 gtgccttcga ggagcccctc cgggtcggga agctccgctc cgtagtagaa accgagcacg
     1381 acatggcccc cgtagggcga aagccaggcg aagtgctcgg tcatcttctt tggtcctgtg
     1441 ccgtagccga ccgtctgcat tcccggccac acgatctcgt acacgccagg catgaactcg
     1501 acgacgaggc gtcgggcggc gcgggctaat tcagcgatgt ccggcccgaa ccgcgacacg
     1561 agcttctcaa acgtctcggc gtccaagcgc tccaccatag ctgtcctcct gcgtgcgcca
     1621 catcgcaccg cgctgacgat tgtacgtgcg cggcttgcgg atctagagct caccgagcta
     1681 cgaggtgctc gtcgctagcc caccgctcgc gggtggtacc tggacgaccg cccagctgtt
     1741 ctaccccgat gtcggatggg gtctcctcgt cgccgggtag gtcgccatcg gcgccgccct
     1801 cgatgcgtac tgcgatcgag gttctcgagg ctcagcgacg tgacgcctga ggagcgcccg
     1861 caggtcaggt gctggcccta gcgcgctacg acgctgaacg ccgagatcga gcggtcgatg
     1921 ttcttgagga tgagctcacc catcggccgc agcgcaattt cgtcaggctg ggtcaggcca
     1981 gcgaccacat cggagctcac gatgaattcg cccgcaccgg ctgaactcga aagccgcgcg
     2041 gcaatgttaa cggtcgtgcc gaagaggtcg ccgtcgcgtt cgataacggg cccggcgtgc
     2101 gcaccgatgt gcgggtcggg cagatcggcg tcccagccct cggttgacaa gagctggcgg
     2161 ccggcgccga ccgcgtcctg aggtgcttcg aagtggagca tggcgccgtc gccgagcagt
     2221 ttgaccagct tgcctcgatg gccgcgtgcc gcccgctcgg cgacgtcccg cagccgagtc
     2281 ccgtacagca cggcggcttc atctccgtgc tgctccgtca gccggctgaa tcccgcgaga
     2341 tccacgaaca ggaccgccgg cggatgctcg ggcggtgccg gggcgatgcc gtgggccgcc
     2401 atcacttcct ccatctgctc gacgccgacc gcagtcatcg cgcgctcgag atagcgctgc
     2461 tcggcccaga ccatgatccg aggcagcagg ccggtcaatc ctcggccgag cacgagcgtg
     2521 aagtcggcgc gctgttgcag cgactcggcg tcaacgccga cgtagcgcat gtgctcggtc
     2581 cacagcgcca tccagccctc gaccattgcc cgcatatgat cgccgaggag ccgtgtggcc
     2641 cgaaggagcg cctcctgctc ccccacccgc atccagaggt cgactagctc ggcgaggcaa
     2701 ccttcctcgt cctcgcgcat gggccgatcg ggtggcggct cggcgagccc gagcgtgtcg
     2761 tagacgcgcg aaaggtgctg agcattcggt cccaggctat cccggtactc ccgataagtt
     2821 cggccgctcg gtccggctgg ctcgaggtag aaggcgtcgg cgtagtcgaa cgtgataagg
     2881 ccctcgtcga cggcccgccg catgtgctcc cacccaaccc cggcgtcaag aaactgcttg
     2941 accgctcgaa cgcgctgaag ctccgcccat gtcagcgtgc cgtcgtcggc ggggcgaatg
     3001 atccctgctt cgatgaaccg gtcgagttcc tgtcgcgtcg ctccgctctc ggcagccgcg
     3061 cgtgacttgg gcagttgcat cgcaaccgat cgtagacctt cccgccgggg cgacgcatcc
     3121 gcgttgttga actgcccccg agccggctgg acgccgtgct gagcggccgt ttgagggtac
     3181 agcaccgcaa tcggccacga aaaagccccg attcacgctc agaatcgggg ctggacgggg
     3241 aaattggtac cgcataccgg attcgaaccg gtgatctccg ccttgagagg gcggtgtcct
     3301 aggcctctag acgaatgcgg cacgtcgtcc cagatgggtc ggaggacgac ctgaagtata
     3361 gcaagcgcct cccggcaggc tcaacccgcc tctagccgtt gctcgtcctg ctgggacccg
     3421 ttcggggcgt gcaaccggtc agcgtgcagc gccagccagc gaggcgttca tgacggccag
     3481 catggcgtgc cgccgctcct ccagccagcc gagcaccgaa ccggcgacct gcggccaggt
     3541 ctccgagccc atcaccagcc cgaagtgcga agccgagggg aagtactcgt aggctgcgcc
     3601 cagcaggtcg gcggtgcggc gcgcctcggt cgggtggatc acgtcgtccc ggccagcggc
     3661 gatcaccagc gccggagcct cgatgcggac gctttccacg ctcaccccca gcatccgctc
     3721 gcgccgagcg ctccccgact cccgtgcccc gtcaagcatc tcctggacct tctccagctc
     3781 cgcatcggtc atgtccggca tcatctcccg ggcgcgctcg ctggacacga tccagcccca
     3841 ccagcgcgcg tcgtataccg gcggcacgtc cttgagctcc atgtcactcg gccgccccgg
     3901 cagcgccgcg gccgggggcg agggcgccag cagcaccagg ccgagcaccg ggttgcgctc
     3961 cgcgtagagc atggcggcca gcgcgccgat tccccagccg atcaccaccg gcggccggcc
     4021 caggtggcgg atggccacgc ccacgtcgtc ggcgtagtcg cgcatcgaca cgctgcccag
     4081 gtcggcgacg tcggaggtgt agtgcccgcg catgttcatg gcgtgcgctt cccagccacg
     4141 ctcggcgaag aaggtcgcga acgacgacca catccaggag ccggtgagcg ccccgtggac
     4201 cagcagcagc gggggcttgc ggctgcgccg ctccggctga tgggtctcca cgtagatccc
     4261 ctcgggatcg atgtagacct cgtcgcgctt gatcgcctgc accatgggag cgcctagtcc
     4321 tccaacgggt tgaagaccat gccggtcagc agcttggggt agaagtaggt cgacttctgc
     4381 gggagcacgt cgccgacggt cgccacccgc gccatctgct ccagcggagt ggggcggacc
     4441 aggatggcgg cgaacgcctc gccggcggcg accgacgcgc gtgcatcggc ctcgctccta
     4501 gtgtaggcca gcggcccacc ggcggcgacc gcggccgcat cgatccccag ccgatcggcc
     4561 aggacggcgg catgcagcac ggccaggtcc agccgggcca cggcagtgct cacccgctcg
     4621 cgccgcatgc ggtcggcgac cacctccggg tcaccgacca tcagccagcc ttccccgtcc
     4681 ggcagcacga ggccgaagat cggctcctcg gccgcgcgga gctcctccag gcgggtggcc
     4741 agctcgtccg gagcgaccgg ctcggcgtgc cacatcggtc caggatccgt gacgagcgag
     4801 cggagcgcat cgggttcggc ctgccggatc aagcggtgcg tcggcaggat gctgagctcc
     4861 tcgcgctcgg cgttgaccat gaccaccatg atccagtcgg cagccagggc gccagctggc
     4921 gcatcggtcc agcgcggatc agcacgcacc tcggcctggt aggccagcgc ggtctcgtac
     4981 cgatggtgcc cgtccgccac gtacagcgtc tggcgagaca ggtagttcag caggcgctca
     5041 tcgggctcca ccgcggccag cgcgtggagc aggcggtcgc cgtcgcgggc gcgccactca
     5101 tcggcccagg cgcggctcat gatgtggggg taccgggccg agcggtcgaa gtacaccgcc
     5161 aggatgggcg acagttgggt gcgcgtggcg tgcagcaggg ccagccgatc cgccttcggt
     5221 ccgggcatgg tgtgctcgtg ggctcgcacc tggtcgccga agggctcgag cagcacgcga
     5281 gccagcacgc cctgcaccgt cggctcgtcc ggtcggtcaa gcgtggcatg cgcgtagtag
     5341 tagaccgatg gctccgccct ccgccggagc gtgccatccg ccagccacgc ctccagcgtc
     5401 gaagccgccg cggcgtgcgg atccggctcg gcggacagct ccagccgcac cgcgtttcgc
     5461 tcgtcgcgag ccagcagctc gcgctgctgc gccggggaga taacgtcgta cggtgggcag
     5521 accaggtccg tcacgtccgc gaccccgcgg ccggccgagg ttggtggctc gtcgagcagc
     5581 cgcacccgct ccggcaccat ctgcggggcg tagcgatcga gcgcgtagga gacgccgggg
     5641 aggggtcgga ccaggggcat gaacagaggc gagtctagcg acgccgggcg acgaagggcg
     5701 gcggctcgtc cgtcaccggg cggagcgcct ccggcacccg tgcgcggcgt ggcaggcgca
     5761 cccgcatgac ggtcccgcgc cctgccgcac ggttgggctc ggcggtgatg tcgccgccat
     5821 gcgagcgaag gatggcacgc gtgatgtaga ggcccaagcc caggccaccg aagcgtcgcc
     5881 caccgccgga cgtctccgcc tccgcgacct ggtagaaccg ctcgaacacc agctcggtgt
     5941 cgcccgggtc gatccccacc ccggtgtcgg ccaccgtcac ctccagccag tcgccgtggc
     6001 cctcggcccg cacctcgacg gggccaccgt ccggcgagta cttgatcgcg ttgtccagca
     6061 ggttggtgag gacctgcccg atgcggtcgc ggtccgcctc caccggcagg ctctccggga
     6121 cgtgcagcgt cacccggcgg tcggtgtgct cccgctcggt gcgggccacc gcctcgcgga
     6181 tctcgtccgc caggtcgatg ctcgccggct caacgctcac gccgcctcgg cggtcgatgc
     6241 ggctcacgtc gagcaggtcg tccaccaggc gccgcatgcg cgccacctgc gagcggatga
     6301 cccgcatctg ggagagctcc ttggcggtgg ccgagtcccg catgcgcgcc tcgagcagct
     6361 gcgagtagcc ctggatgctg gtcagcgggg ttcgcagctc gtggctgacg atggacagga
     6421 agtcgtcttt cacccgctgt gcacgctgct cgcgcgcgac ctgctcctgg aggtcggccc
     6481 acagcgcggc gttctccagt gccggcccgg ctcgctcggc gatggccatg gccagccgcg
     6541 tctcgccctc gcccatggtc gggccaccgg cgctggccgt gaccagaacg ccccatacct
     6601 gcccccgcga cacgatgggt gccgccacga gggagcgcag gcccagcgcc gccacgccgt
     6661 ccgggtcacc ggggtgcacc ggcacggggt tctggccacc gaccaccacc ggcagcacgg
     6721 tggccgccgc cgcgccggcc agcccctcgc ccatgcgcag ggagcgcttc ccgacgtggg
     6781 ccaccagctg ctccacgcgc tgcgcgtcgc gcccgtcggc ggcagccagc gtcaggctcg
     6841 gctgctgacc ctcgccgtga tcggtctcca ccagcagcag ccagctcagc tcgccgagca
     6901 gcggcgcggc agcgcggatc acggcgtcgg ccgtggtgtg ctggtccagc gagctgccga
     6961 actcgcgcgc cagctggctc agcagctcca cctccctggc gcgcgcccgc tcgcgctcct
     7021 cctccgccag gcgcagctgc agccgggcgt tctcctccgc ccgacccatc accgctcgcg
     7081 cgaagaggcc ggcggtcagg cccagcccga gcaggtaacc gatgcggacg ccgagcagct
     7141 cccgggttgg cgtgccgggc tgcgccgcga ggatcaccac cgagccgtac acgaatgaga
     7201 ccacgacggc ggaccacagc gccgcgccga agccgaagcg cagggccacg ccgagcacga
     7261 ccgggaagta gaagacgaag gcgtcgatgg cgcgaggctc gacgtagatg gccagcgtga
     7321 ccacgaccat gtcggcggcc gtggccacca ccccgacctc tcgcgccggg aagcgcccga
     7381 ccagcagcat ggtcgccagg ctgaacgcca ccgccgcgag gcccagcccc agcagccacg
     7441 ggaaggctgc cagagcctct cggaagatga cgaccgccac cagggcggct cccacgattg
     7501 ccaggcgggc cactgcgagc tgccgctcct gcaggcgcag ccgccgctcc aggtgctctt
     7561 cgaggtcggt ggggcgggcc cgggtggcgt ccatcggcag ccgatcgtca cgggattgga
     7621 atggccggcc gactatagca tcggggcatt cggcgcccct acactcgccg cggaccgtgc
     7681 aaccccgccg gcccgtgcgg cgtcttgacc aggcaggccg ccccgaacgc agagtgagca
     7741 tgacccgaag accgaccgcc gccctcgcca tgctggccac gctgctggcc gtcacggtgc
     7801 tggtcgcctc gcccggttac ggcctggccg cggatcccgt cgcgatggag gcgcgcgccc
     7861 aggtggccgg ccgcttcgag gctggaggct gggcagccat ctcggtgcgc ctggcgaacg
     7921 acggcgagcc agtgaccggc tacgtcgccg ccgacggcga ggacgggacg gtgcggcggc
     7981 ttgtagagct tcccgccggg tcgcgcaagg aagtcaccct ctacctgcga cccgagagct
     8041 tcgcccggac ggtcgccgtg cgcttcgaga ccagcgacgg cgaaccggtg gcgtcggcgg
     8101 aggccgacgt ggcggtcctc gagcggacca gcgccacagt ggcggtggtc ggtgacgtga
     8161 ccggcatcct tcgcccccaa ctgatggcgc gcgacaccgg catcccggag ccggtggcca
     8221 tgacgccggc cgacatcacc gagcgcccgg aaccgatgcg cgggctggag accatcctgt
     8281 gggccggaga ttcgagcacg ctgaccgagg cgcagcgacg gtcgctcgag cgctggatcg
     8341 ctgccggtgg acagctggtg gtggtcggcg gtccggactg gcaggcgcgg actgcggctt
     8401 tcgaagcctt ccttccggtc gaggatctgc gttccgtcga cgaggtcggc ctggctccgc
     8461 tggccacctg ggtcgcgcac gaacttcccc ccgagggcgt ctccgcctcc gtatcgcttg
     8521 ggcagctgcg ctccgatgcg gtggccctgg tcggcaccgg cgacgacgtc ctgctggcgt
     8581 ccgtcgtgcg cggcgcgggg cgggtcagct ggctgggcgt ggacctggcc accgaggcct
     8641 tccgtgcctg gcccggggcg ccggggctct ggcagcggct gctgccgaac gaccgcgcgg
     8701 cggagcaatt cggggccgtg ccgccgctcg aggaggaggt cgccggcctg atggtccagg
     8761 cgctctccaa cctgcccgcc ctcgacgtcc cgccggcgga actgctgctc gccgtgctgg
     8821 tgggctacat cctgctcatc ggtccattga gctacctcgc gctgcgccgg ctcgatcggc
     8881 gcgagctggc ctgggtcacg gcacccatcc tggtcctggt cttctcgtcg gtgagctacg
     8941 ggatcggcgc cagcatgaag ggcagcgacg tcatcctcaa cgaggtcgcc gtggtgcgca
     9001 caacgaccga tgggaccgcg gcatcggtct ccagcttcgc cggcgtcttt tcgcccacgc
     9061 gtcgcgccta cgacatcacg gtggccgggg aggcgctctt ctcgcgcctg gtcaacccgt
     9121 tcgatggcac ccagcgcggt ggcggcctgg cgacagaaca gggccaccca tcgcggctgc
     9181 gagggctctc cgtgagcgtc ttcgggctgg agggcattcg agccgatacg ctcatcccct
     9241 acaccccggc gctgtcggtc acctggtcct tcaccagcga aggtattcgc ggcagcgcga
     9301 ccaacaccgg cgaggagccg atggaggacg tcgcgctcgt caccgagggc ggcggcgcca
     9361 tggtcgggac gctggacgcc ggccagacga aggagttcgc cctctcgatg cggggtgcca
     9421 accagcgctc ggcgtccgag cagatctatg ggcaggtgtc gggggtgagc ggctcggata
     9481 cccagcgccg catcgccctg cgccgccagg tgatcgacac tttggtcggt tatggcggat
     9541 ggaccggccg cctcggcggc gggctgagcg gcatcgaccg cggaccgtac gtcatcggct
     9601 ggcgccaggg cgccgcgccc gtcgccatgg aggtggatgg cgagcgcgcg cagcactacg
     9661 tgcaggcggt ggaggtcgtc agcggccggc cgcggctggg gccaggcgcg gtgaccatcc
     9721 tgccggccca catctcgacg gacgtcctcg agctgatcgg cgacgccaac gaggccgagc
     9781 ccggctacgt ggtggtcggc gagggcgagg cggtgttcgg catgacgctg ccgctggagg
     9841 cgaccggcct cgccgcgagc tccatcaccc tgctggctgg gagcgatccg agcatggtgg
     9901 tctccgacca accgaacttc ggggcagcgc tgccgcccgg ctacaccctc agcgtgtggg
     9961 acaacctgtc gagctcgtgg gaggaggttg gtgacctggc ggcgcgcagc cggttcgagc
    10021 tggagcagcc tggcggtctg atcgacgcca gcgggcggat cgtggtcaag atcaccggca
    10081 ccgaggtgga tccgaacttc ggcgccagcg gcgtcttcgt cggagccgtg gtggaggggg
    10141 tgatctgagc gtgatgcgac cggtgatcga aacgcgcggc ctggtcaagc gctacgacgg
    10201 cgagacggcg gtggcaggtg tggacctcgt ggtccagccc ggcgagatct acggcctggt
    10261 cggccccaac ggcgcgggca agaccacgac catgcgtatg ctggccaccc ttctggcgcc
    10321 gacagccggc caggccgtgg tgtgtggcgt ggacgtcatg gacgacccgg tcgaggtccg
    10381 gcgccgcatc ggctacatgc ccgacttctg gggcgtgtac gacgaccttc gcgtgtggga
    10441 gtacctcgac ttcttcgcgc gttgctacgg cgtaccgacc agccggcggg catccgtcat
    10501 cggcgagctg ctggagatcg tggatctcgg cgacaagcgg gacgcctacg tggagtcgct
    10561 gtcgcgcggc atgcggcagc ggctgtgcct ggcgcacacc ctggtccatg accccgagct
    10621 gctgatcctg gacgaacccg cctcgggcct cgacccgcgg gcgcgcgtcg agatgcgcga
    10681 gatcctgcgc gccctgtccg gcatgggcaa gacgatcctg gtcagcagcc acatcctgcc
    10741 cgagctcggg gagctgtgca cggcggtggg catcatcgac cacggccgtc tgctgcggtc
    10801 cgggcgtatc gccgacatcg agcgctcgct ccaggcgacg gcacagctgc gcatcgacct
    10861 gctgtccaac ctggagcagg cggtcgcctt cctgggaacc gatccgcggg tcagcagcgt
    10921 ccttgagatc gagggaggca tcggtaccgc gagggtggag gtctccttcg atggtggtcc
    10981 ggaggagcag tcggcgctgc tgcaagccat gatcgaggac ggacaccggg tggccggctt
    11041 cgggcaggcg accagcggac tggaggagat cttcctgcgc gtgacgggca gcgtggcggg
    11101 cgacgaggag acggcggcat gacggcagcg agcacggcga ccggcggcac gcgtcgcggc
    11161 gacctgcggg agctggcctc gtcggccgcc accatcatgg tcaaggagct gcgctcgcgc
    11221 ttccgcgggc gtcgggcctt cctggtgctg accctctacc tggccgtgct ggcggcgctg
    11281 gcctacggca tgtacacggt gaccgggccg gccgctcgca acgcagccca ggtgggcgcc
    11341 cagttcggag atccaagcgc catcaacgcc tcggcccgcg tgggccaggg catcttcagc
    11401 atcctctcaa tcttccagct gctgttgatc gggttcatcg cgccggcctt cacggccggt
    11461 tccatcagcc tggagcgcga gaagcagacg ctcgacctcc tgatcgcgac ccccatccgt
    11521 ccggccggcc tggtgatcgg caagctgttg agcgccctcg ccttcgtggt gctcatgatc
    11581 gtggccgcca tcccgatctc ggcactggtg ctcatgtacg gcggcgcctc gatcgacgac
    11641 atcctgcgcc agcaggccgt gctcttcgca tctgccatcg gtttcggcgt catcggcctg
    11701 ttctggtcgg ccctgaccaa gcggacccag gcggccaccg tgctcaccta ctccaccctg
    11761 ctggtggtca ccatcgggac cctgctggtc tggcgcttct ggaccgatgt cgccaccgcg
    11821 gcccctgaca acactttcgg agaggtgcgc accgcccccg aggagctgct gtacatcaac
    11881 ccggcggtgg ccatgatcga gatcgtggcc aacaccgaga tcgagtacgg cgacttcagc
    11941 gaagcctacg acgcccttcg cggcggagcc ggcaacgtgg tgtgcgacgg ggacgtctgc
    12001 ttcccggcct ccgatggccg attcgtgccg ttggcaccac cgggggccga cttcgacgag
    12061 gagggccggg tgccgatggg catcgacttc gccgtgcccc cggatcggct caccgtgaac
    12121 gacggcgtct tcaaccactt ctggcctcgc atcaccgcca cctatgccgc gctatcggtc
    12181 ctgctgacgc tggccgccat gcgcctcgtc cagccggccg gcacccgcct gcgcttcgcg
    12241 ccacggcgtc gcagctccga cccggatacc gccgtgccgc tcgaggccga gggcagcatc
    12301 gaggagatgc gcccgtgacc gaccgacagc tggctgatgc gcttcgcgcc cggctcggtg
    12361 acgacatggt cgctcgcctt cgtaagctgc gccgccgcct gtggctgcgc cgcgcggtgc
    12421 ggatcggggc actcaccacg atcgccgtgc tgggactgct ggccgccgtc cagctcgtgg
    12481 cgcgcagcat gccgctggag gccgcgccct ggctgatggc tgccgcgggg ggcttcgggc
    12541 tcttggcctg gctggctgtg agctggagcc ggcgtccaag tctgctggag acggcgcacc
    12601 gggccgatga ggagctcgcg cttcgtgagc gtctgggaac ggcggccgag ctggttctgc
    12661 gaccggacga ccgtgacgtc ccggcggacg tcgtcacgct ggagggcagg cagctggccg
    12721 atgcgcgcgc ccgactcgcc gaggtcgagc tgaatcgagc tttccgaccg cggctctcac
    12781 gccgtgggct ggcggccggc ggtattgcgc tcgccctgct gctggtgctc accgtgtggc
    12841 cgaatccgca ggacggcatc ctgcgcgacc ggcaggcggc acgtgatgcc agtcgggacg
    12901 tcgccgaacg gatcgatgac gtggcggatg aggccgagcg ccgcggggcc gagaccgacg
    12961 acccgcgtcg cgatgagctg gtggagcggc tgcgcgagct ggcacggaag ctccgagaat
    13021 ccggggac
//
LOCUS       DATACA010000419         8814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1422323, whole genome shotgun sequence.
ACCESSION   DATACA010000419 DATACA010000000
VERSION     DATACA010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422323"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..4481)
                     /gene="rpoC"
                     /locus_tag="VHK28_08605"
     CDS             complement(39..4481)
                     /gene="rpoC"
                     /locus_tag="VHK28_08605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX2142313.1"
                     /translation="MLEVNNFDAIRISLASPDQIKSWSSGEVTKPETINYRTLKPEKD
                     GLFDERIFGPTKDWECYCGKYKRIRYKGIICDKCGVEVTRSKVRRERMGHIKLASPVS
                     HIWYFKGTPSRLGILLDISPRNLERILYFAMYVVNRVDEAEREKALARIDEEMAERIA
                     RAEAVVTEKRAELESAIADKRAELEAAYEADVRRHDERFAARSEELATAAQQVEAALA
                     EGGTKVAEDLVFTPTGEVVARAGESAKQALTALRKVVKAEVEALDAEVKQAKAEAKER
                     LDGAIGDLTGGIDSALVTEREQVARETDDARAAARSARQQLNDIKIMQTLTESEYRNL
                     SDQYGRMFDAGMGAEAVKRIIEQLNLDDLSQKLHVEMRQTSGQRRKKAIKRLRLIEAF
                     RKSGARPEWMILTTLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLSRLLDL
                     EAPEIIIRNEKRMLQEACDALIDNGRRGRAIAGTGNHRLKSLSDMLKGKQGRFRQNLL
                     GKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVMRQLVEKGFAHNIKSAKRIV
                     ERVRPEVWDVLEDVIKDHPVLLNRAPTLHRLGIQAFMPVLVEGSAIQIHPLVCFAFNA
                     DFDGDQMAVHVPLSTAAQREAKELMLSTQNLLSAADGSPVVSPTHDMVLGCYYLTIES
                     PDAKGAGRVFSSAAEATMAAQLGHVHVQAPIRVELELPVSPETDGADGTDGAAVRREI
                     VDTTAGRVIFSTVIPPQLGFLNKVMDRKELKALIAQCYRQLGPEATTGLVDGIKSLGF
                     HYATVAGITIGIDDMSVPVEKAALLSDADDRVADIDREFRKGFITEEERYNQTVDVWR
                     ATTDDVTSKMLEGLDKRGSVWMITHSGARGNVTQVHQLAGMRGLMADPSGRIIDLPIR
                     SNLREGMSVLEYFISSHGARKGLADTALRTADSGYLTRRLVDVAQDVITREDDCGTDL
                     GTWITRDESVEIPEPFGDRLVGRLAALDVTDEETGELLLSRNGELDEAVVARIEASSI
                     DRVNVRSPLTCAARHGVCRQCYGRDLATGRLVELGQAVGIIAAQSIGEPGTQLTMRTF
                     HTGGVAGEDITQGLPRVEELFEARIPKGQAIMSEIDGTVEVVRTGPDQPITVLVTHRE
                     SYDTPFKVTPQHEVLVANGDEVSEGQLLARIGEGEDVVEIKAPSAGRVTKKGTTLTLH
                     SEDVDQREYAVPHSARIRVETGDEVNAGDALTEGSLNPKDLLEIKGVDTVQRFLVAEV
                     QKVYRSQGVTIADKHIEIIVRQMLRKVTVDSPGDTDLLPSELIDRFEFEEVNNRILAE
                     GGEPATAQTVLLGVTKASLNTSSFLAAASFQETTRVLTEAAINGAKDKLMGLKENVII
                     GKLIPAGTGAAARALAAQRAREIPPEEAEALREREAAMTFLTGDADADQDGTVEPAEA
                     EAAAEEAGDSLAELMGADEDPSIEAEVKEQVFDELSEGASAKKK"
     gene            complement(4606..8088)
                     /locus_tag="VHK28_08610"
     CDS             complement(4606..8088)
                     /locus_tag="VHK28_08610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX2142314.1"
                     /translation="MALAESASRLELAPRRESFAHFPEVLPLPNLIQTQTDSFHWFIE
                     NGLRELLDEITPITDFTGKNLELHFRDYYFEEPKFSEEECRTRDLTYSRPLKVEVDLV
                     IKETDEVKHQTVFMGDFPWMTEQGTFIINGAERVVVSQLVRSPGVYYTGSEDPATGRM
                     LYAAKVIPNRGAWLEFETSNKDLLSVKVDRKRKIPVTTLLRAVGFDDSSEMVRLFSSV
                     DDNPEHPYVQLTLDKDTAQSQDEALIEVYKKLRPGDPPTVENARALVTSLFFNFRRYD
                     LGRVGRYKLNKALADVAAKLKIDLPSESGSNLRDARVISRNDIVAIIGKLIELNNGRG
                     MGDDIDHLGNRRIRANGELIQNQFRIGLLRMERVVKERMTITDADQATPNTLINIRPV
                     VAAMKEFFGGSQLSQFMDQTNPLAELTNKRRLSALGPGGLSRERAGFDVRDVHPSHYG
                     RMCPIETPEGPNIGLIGSLATYGRINSYGFIETPYRKVRKLIGHKDKQADALGQVLAE
                     DLVSGDGRKPLAKAGSLVDEKVWAAIKEHKIAGVRVRPVVTDEIEFLAADEEEKYYIA
                     QANAPLDDEHHFEEERVMVRYRDEFRVQPAEDVDYMDVSPKQIVSVATAMIPFLEHDD
                     ANRALMGSNMMRQAVPLLQPESPVVGTGVEFRAALDLQAVMVARNAGTVVSVTADQII
                     VERDEGNERDTYKLNKFLRSNQGTCINQRPIVDVGTRVGEGQVLADSSSTDQGEMALG
                     QNILVAFLPWEGGNYEDAIVISERLVREDLFTSIHIEKHEIEARDTKLGPEEITRDIP
                     NVGEESLRNLDEDGIIYEGAEVTPGDILVGKITPKGETELTAEERLLRAIFGEKAREV
                     KDSSLRLPHGERGIVVDVRQFSRDNGDELLPGVNRLVRVSVAQKRKIAVGDKMAGRHG
                     NKGVIAKILPVEDMPFMPDGTPVDIVLNPLGVPSRMNIGQVLETHLGWAMKVLGLQAA
                     TPVFDGATEEHIRERLVQAGLAEDGKTVLFDGRTGEAFDHQVTVGYIYMLKLHHLVED
                     KIHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQELLTVKSDDVV
                     GRVQTYEAIVKGEEIQPPGVPESFKVLVKELQALGLNVEILNENEEEIRFIEDTGSYP
                     LPDLGGINLQGFEE"
     gene            complement(8376..>8814)
                     /locus_tag="VHK28_08615"
     CDS             complement(8376..>8814)
                     /locus_tag="VHK28_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX2142315.1"
                     /translation="ASDGALLNEIASAAGRGAWAIHHLSIGALAALHERARVVVGMDS
                     GALHLAAAVGAPVVGLYGPFGADRVAPLGPVTRVRAIWRSLPCSPCGTLEAPPCGASH
                     DPPCLLAVSVEDVIQSAVELDGMSDAVGRAWLPPDSSIGALGT"
BASE COUNT         1240 a         2988 c         2973 g         1613 t
ORIGIN      
        1 gggcgccccg ccctcatcgg tccatccgtt gccgaaggtc acttcttctt ggccgaggcc
       61 ccttcggaga gctcgtcgaa gacctgctcc ttgacctcgg cctcgatgga gggatcctcg
      121 tcggcgccca tcagctcggc caggctgtct cccgcctcct cggcggcggc ctcggcctcg
      181 gccggctcca cggtgccgtc ctggtccgcg tccgcgtctc ccgtcaggaa ggtcatggcc
      241 gcctcgcgct cgcggagcgc ctcggcctcc tccggcggga tctcccgggc tcgctgtgcg
      301 gccagcgcgc gggcggcagc gcccgtgccg gcggggatca gcttgccgat gatcacgttc
      361 tccttgaggc ccatcagctt gtccttggcg ccgttgatgg ccgcctcggt cagcacgcgg
      421 gtcgtctcct ggaagctcgc ggccgccagg aaggaggacg tgttcagcga ggccttggtg
      481 acgcccagca gcacggtctg cgccgtggcc ggctcgccgc cctcggccag gatccggttg
      541 ttgacctcct cgaactcgaa gcggtcgatc agctcggacg gcagcaggtc ggtgtcgccc
      601 ggcgagtcca ccgtgacctt gcgcagcatc tgccgcacga tgatctcgat gtgcttgtcg
      661 gcgatcgtca cgccctggga tcggtagacc ttctggacct cggcgaccag gaagcgctgg
      721 acggtgtcca cgcccttgat ctcgagcagg tccttcgggt tcagcgagcc ctcggtcagc
      781 gcgtcgccgg cgttcacctc gtcgccggtc tcgacccgga tgcgggccga gtgcggaacc
      841 gcgtactcgc gctggtccac atcctccgag tgcagcgtca gggtggtgcc cttcttcgtc
      901 acccgcccgg cggacggggc cttgatctcg accacgtcct caccctcgcc gatgcgggcc
      961 agcagctggc cctcgctcac ctcgtcgccg ttggccacca ggacctcgtg ctgcggggtg
     1021 accttgaacg gcgtgtcgta tgactcgcgg tgcgtgacga gcacggtgat cggctggtca
     1081 gggccggtgc gcaccacttc gacggtgccg tcgatctcgg acatgatcgc ctggcccttg
     1141 gggatgcgcg cctcgaatag ctcctcaacg cgcggcaggc cctgcgtgat gtcctcgccg
     1201 gcaacgccgc cggtgtggaa cgtccgcatg gtcagctgcg tgccgggctc accgatcgac
     1261 tgggcggcga tgatgcccac cgcctggccc agctccacca gccggccggt cgccaggtcg
     1321 cggccatagc actggcggca caccccgtgg cgtgcggcgc aggtcagcgg cgagcgaacg
     1381 ttgacgcggt cgatggacga cgcttcgatg cgcgccacca cggcctcgtc cagctccccg
     1441 ttgcgggaga gcagcagctc gccggtctcc tcgtcggtga cgtccagggc ggccagccgg
     1501 ccgaccaggc ggtcaccgaa cggctccggg atctcgaccg actcgtcgcg ggtgatccag
     1561 gtgcccaggt ccgtgccgca gtcgtcctcg cgggtgatga cgtcctgcgc cacgtccacc
     1621 aggcgccggg tcaggtaccc ggagtcggcg gtgcgcagcg ccgtgtcggc cagtcccttg
     1681 cgggcgccgt gactcgagat gaagtactcg agcacgctca tcccctcgcg gagattggag
     1741 cggatgggca ggtcgatgat ccgccccgat gggtcggcca tcaggccgcg catgccggcc
     1801 agctgatgca cctgggtcac gtttccgcgc gcgccggagt gggtgatcat ccacaccgag
     1861 cctcgcttgt cgagaccctc gagcatcttg ctggtcacgt catcagtggt ggcgcgccag
     1921 acgtcgaccg tctggttgta gcgctcctcc tcggtgatga agcccttgcg gaactcgcga
     1981 tcgatgtccg ccacccggtc atcggcgtcc gacagcagcg cggccttctc caccgggacg
     2041 ctcatgtcgt cgatgccgat ggtgatgccg gccacagtgg cgtagtggaa gcccaggctc
     2101 ttgatgccgt ccaccagccc ggtggtggcc tccgggccga gctggcggta gcactgcgcg
     2161 atcagcgcct tgagctcctt gcggtccatg accttgttca ggaaaccgag ctgcggcggg
     2221 atgaccgtgc tgaagatgac gcgaccggcg gtcgtgtcga cgatctcgcg ccgcaccgcg
     2281 gcgccatcgg tcccgtccgc gccgtccgtc tcgggtgaga cgggcagctc gagctcgacc
     2341 ctgatggggg cctgcacgtg gacatgtccc agctgggcgg ccatggtggc ctccgccgcg
     2401 ctggagaaga cccgcccggc gcccttggcg tccggcgact cgatggtcag gtagtagcag
     2461 cccagcacca tgtcatgggt gggcgacacc accggcgagc catcggccgc cgagagcagg
     2521 ttctgggtcg agagcatcag ctccttcgcc tcgcgctggg cggcggtcga cagcggcacg
     2581 tggaccgcca tctggtcgcc gtcgaagtcg gcgttgaagg cgaagcagac cagcgggtgg
     2641 atctggatgg ccgatccctc gaccagcacc ggcatgaagg cctggatgcc cagccgatgc
     2701 agggtcggag cccggttgag gaggaccggg tggtccttga tcacgtcctc cagcacgtcc
     2761 cacacctcgg gccgcacgcg ctccacgatc cgcttggcgc tcttgatgtt gtgggcgaag
     2821 cccttctcca ccagctgccg catgacgaac ggcttgaaca gctcgagcgc catcttcttg
     2881 ggcaggccgc actggtggag cttcagctcc gggccgacca cgatgaccga gcggcctgag
     2941 tagtcgactc gcttgcccag caggttctgc cggaagcgac cctgcttgcc cttcagcatg
     3001 tccgaaaggc tcttgagccg atggttgccg gtgcccgcga tggcacggcc acggcggccg
     3061 ttgtcgatca gcgcgtcgca cgcctcctgg agcatccgct tctcgttgcg gatgatgatc
     3121 tccggcgcct cgaggtcgag gagccgcgac aggcggttgt tgcggttgat cacccggcgg
     3181 tagaggtcgt tcaggtcgga ggtggcgaag cgaccaccgt ccagctggac catgggccgc
     3241 aggtccggcg gaatgaccgg cagcgtggtc aggatcatcc actccggtcg cgccccggac
     3301 ttgcggaagg cctcgatcag gcgcaggcgc ttgatcgcct tcttgcgtcg ctgccctgag
     3361 gtctggcgca tctcgacgtg cagcttctgg gacaggtcgt ccaggttgag ctgctcgatg
     3421 atgcgcttga ccgcctcggc gcccatgccg gcgtcgaaca tgcggccgta ctggtcgctc
     3481 agattgcggt actccgactc cgtcagcgtc tgcatgatct tgatgtcgtt cagctgctgc
     3541 cgggcggacc gcgcagcagc gcgggcgtca tcggtctcgc gagccacctg ctcccgctcg
     3601 gtgaccagcg cggagtcgat gccgccggtc aggtcgccga tggctccgtc cagccgctcc
     3661 ttggcctccg ccttggcctg cttgacctcg gcgtcgagcg cctcgacctc ggccttgacc
     3721 accttgcgca gcgcggtcag ggcctgcttg gccgactcgc cggctcgcgc caccacctcg
     3781 ccggtggggg tgaagaccag gtcctcggcc accttggtgc caccctccgc gagggcggcc
     3841 tcgacctgct gtgcggcggt cgccagctcc tcggagcgtg cggcgaagcg ctcgtcgtgg
     3901 cgacgcacgt ccgcctcgta ggcggcctcc agctcggcgc gcttgtcggc gatggcagac
     3961 tcgagctcgg cgcgcttctc ggtgacgacg gcttctgcgc gcgcgatgcg ctcggccatc
     4021 tcctcgtcga tgcgagccag cgccttctca cgctccgcct cgtcgacgcg gttgaccaca
     4081 tacatggcga agtagaggat gcgctccagg ttgcgcgggc tgatgtcgag caggatgccc
     4141 aggcgcgacg gcgtgccctt gaagtaccag atgtggctga ccggcgaggc cagcttgatg
     4201 tggcccatcc gctcgcggcg caccttggaa cgggtgacct ccacgccaca cttgtcgcag
     4261 atgatcccct tgtagcggat gcgcttgtac ttcccgcagt agcactccca gtccttggtg
     4321 ggaccgaaga tgcgctcgtc gaagaggccg tccttctccg gcttgagcgt gcggtagttg
     4381 atcgtctccg gcttggtgac ctcgcccgac gaccagctct tgatctgatc gggcgacgcc
     4441 aggctgatac ggatggcgtc gaagttgttg acttccagca tgagctgcta cgactcctgc
     4501 ggcactgtcg gccgcgactc gggcgtctgg ccaggggctg accaggcccc ggcaggtgat
     4561 ggaccgatgg tttcgcgcgg cgccccggtg gacgccgcgc cgtgcttact cttcaaagcc
     4621 ctggaggttg atgcctccca gatccggaag cggatagctt ccggtgtcct cgatgaagcg
     4681 gatctcctcc tcgttctcgt tgaggatctc cacgttcagg cccagcgcct ggagctcctt
     4741 gaccaggacc ttgaatgact ccggcacgcc gggcggttga atctcctcgc ccttgacgat
     4801 ggcctcgtag gtctggaccc gtcccaccac gtcgtcgctc ttcacggtga gcagctcctg
     4861 gaggatgttc gcggcgccgt acgcctcgag cgcccagacc tccatctcgc cgaaacgctg
     4921 gccgccgaac tgtgccttgc cgcccagcgg ctgctgggtg atcaggctgt aggggccggt
     4981 gctgcgggcg tggatcttgt cctccaccag gtggtggagc ttgagcatgt agatgtagcc
     5041 gacggtgacc tggtggtcga acgcctcgcc ggtgcgaccg tcgaagagga ccgtcttgcc
     5101 gtcctccgcc aggccggcct ggaccaggcg ctcccggatg tgctcctcgg tggcgccgtc
     5161 gaagaccggc gtcgccgcct ggagccccag gaccttcatg gcccagccga ggtgcgtctc
     5221 gagcacctgg ccgatgttca tccggctcgg cacgccgagc gggttgagga cgatgtcgac
     5281 cggcgtgccg tccggcatga agggcatgtc ctccaccggg aggatcttgg cgatcacgcc
     5341 cttgttgccg tggcggccgg ccatcttgtc gcccaccgcg atcttccgct tctgggccac
     5401 gctgacgcgg accaggcggt tgacgccggg cagcagctcg tcgccgttgt cacggctgaa
     5461 ttggcggacg tcgaccacga tcccgcgctc gccgtgtggc aggcgcaggg aggagtcctt
     5521 cacctcgcgg gccttctcgc cgaagatcgc gcgcagcagc cgctcctcgg ccgtcagctc
     5581 cgtctcgccc ttcggcgtga tcttgccgac caggatgtca ccgggcgtga cctcggcgcc
     5641 ctcgtagatg atcccgtcct cgtccaggtt gcgcagcgac tcctcgccca cgttcgggat
     5701 gtcgcgggtg atctcctcgg ggcccagctt ggtgtcccgg gcctcgatct cgtgcttctc
     5761 gatgtggatg gaggtgaaca ggtcctcgcg caccagccgc tcgctgatga cgatggcgtc
     5821 ctcgtagttg ccgccctccc atgggaggaa ggccaccagg atgttctggc ccagcgccat
     5881 ctcgccctgg tccgtgctgg agctgtcggc cagcacctgt ccctcgccga cgcgggtgcc
     5941 cacgtccacg atggggcgct ggttgatgca ggtcccctgg ttggagcgca ggaacttgtt
     6001 gagcttgtac gtgtcgcgct cgttgccctc gtcgcgctcg acgatgatct ggtcggcggt
     6061 cacgctgacc accgtcccgg cgttgcgcgc caccatcacc gcctgtaagt cgagcgccgc
     6121 gcggaactcg acgccggtgc ccaccaccgg cgactccggc tggagcagcg ggacggcctg
     6181 gcgcatcatg ttcgagccca tcagcgcacg gttggcgtcg tcatgctcca ggaacgggat
     6241 catggcggtc gccacgctga cgatctgctt ggggctgacg tccatgtagt cgacgtcctc
     6301 cgccggctgc acccggaact cgtcgcggta gcggaccatg acccgctcct cttcgaagtg
     6361 gtgctcgtcg tccagcggcg cgttggcctg cgcgatgtag tacttctcct cctcgtcggc
     6421 ggcgaggaac tcgatctcat cggtgaccac cgggcgcacg cgtacgccgg cgatcttgtg
     6481 ctccttgatg gcggcccaca ccttctcgtc gaccaggctg ccggccttcg ccagcggctt
     6541 gcgcccgtcg ccggacacca ggtcctcggc cagcacctgg ccgagcgcat cggcctgctt
     6601 gtccttgtga ccgatgagct tgcgcacctt gcggtagggc gtctcgatga agccgtagct
     6661 gttgatgcga ccgtaggtgg ccagcgagcc gatgaggccg atgttcggcc cctccggggt
     6721 ctcgatgggg cacatccggc cgtagtggga gggatgcacg tcgcgcacgt cgaacccggc
     6781 ccgctcgcgg gaaaggccgc cgggacccag cgccgacagg cggcgcttgt tggtcagctc
     6841 cgccaggggg ttggtctggt ccatgaactg ggagagctgg ctgccgccga agaactcctt
     6901 catggccgcc accaccgggc ggatgttgat cagcgtgttg ggcgtggcct ggtcggcatc
     6961 ggtgatcgtc atccgctcct tgaccacgcg ctccatgcgg agcaggccga tccggaactg
     7021 gttctggatc agctcgccgt tggcccggat gcggcggttg ccgaggtggt cgatgtcgtc
     7081 gcccatgccc cggccgttgt tcagctcgat cagcttgccg atgatggcca cgatgtcgtt
     7141 gcggcttatg acccgcgcgt cgcgcaggtt gctgcccgac tccgagggca ggtcgatctt
     7201 cagcttggcg gcgacgtcgg ccagcgcctt gttcagcttg tagcggccga cccggcccag
     7261 gtcgtagcgg cggaagttga agaacaggct ggtgaccagc gcccgcgcgt tctcgacggt
     7321 gggcgggtcg ccgggacgca gcttcttgta gacctcgatc agcgcctcgt cctggctctg
     7381 cgccgtgtcc ttgtccaggg tgagctggac gtaggggtgc tccgggttgt cgtcaacgga
     7441 cgagaagagg cgcaccatct cgctgctgtc gtcgaagccg acggcacgca gcagggtggt
     7501 gaccggtatc ttccgcttgc gatcgacctt gacgctgagc aggtccttgt tgctggtctc
     7561 gaactcgagc caggcgccac ggttggggat gaccttggcc gcgtacagca tgcgaccggt
     7621 ggccggatcc tcggaaccgg tgtagtagac gcccggcgag cggaccagct ggctgaccac
     7681 gacgcgctcg gcgccgttga tgatgaaggt gccctgctcc gtcatccacg ggaagtcgcc
     7741 catgaagacg gtctggtgct tgacctcgtc ggtctccttg atcaccaggt cgacctcgac
     7801 cttcagcggt cgcgagtagg tcaggtcgcg ggtgcggcac tcctcctcgc tgaacttcgg
     7861 ctcctcgaag tagtagtcgc ggaagtgcag ctcgagattc ttgccggtga aatcggtgat
     7921 cggggtgatc tcatcgagca gctcgcgtag gccgttctcg atgaaccaat ggaacgaatc
     7981 ggtctgggtc tggatcaggt tgggaagcgg gaggacctca ggaaaatgtg cgaagctctc
     8041 acggcgcggg gccagctcaa gccggctggc agactcggcc aaagccattc acgactccta
     8101 gcggaaacag ggtggagggc atggtggggg cgcggacacg cgaacgagca gtatatcact
     8161 cgtttccgct tcgtcaactg aagcgccctg ggtgcaaaac gccagacctt tctatcggtc
     8221 catcgggcac cttagagggg atttctggct cctctgacgg catcgtgagg ccgtctgacc
     8281 gatctataca tgcacgtgac acctgagcgg gcaatcgcga gtcccctgcc tgcatctatc
     8341 ggtgcaaacg tcgcatgagg gcggttttcg gcctcctatg tgcccagtgc cccgatagat
     8401 gagtcaggcg gcagccaagc acgcccgacg gcgtcggaca tcccgtcgag ctcgaccgcg
     8461 ctctgaataa cgtcttcgac ggacacggcg agcagacatg ggggatcgtg cgaggcaccg
     8521 cagggtggtg cttcgagcgt cccacacggg ctgcacggca gggatcgcca gatggcccga
     8581 actcgggtca ccggcccgag cggcgccact cggtcggcac cgaacgggcc gtacaggccc
     8641 accaccggtg cgcccaccgc ggcggccagg tgcagggcgc cgctgtccat gccgaccacc
     8701 acgcgagcac gctcatgcag cgcggctagg gcgccgatgg agaggtgatg aatggcccac
     8761 gcgccccggc cggcggcgct agcgatctcg ttcagcagcg cgccatcact cgcc
//
LOCUS       DATACA010000420         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_137259, whole genome shotgun sequence.
ACCESSION   DATACA010000420 DATACA010000000
VERSION     DATACA010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137259"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..722
                     /locus_tag="VHK28_08620"
     CDS             84..722
                     /locus_tag="VHK28_08620"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX2142316.1"
                     /translation="MSLIHEIPPEPQRSDELFVAFANTLHHERGAPVDDIGDLDALLA
                     WLRDHHLLSGRARATETARLHRDPGDAGRRLQRFRRLRDVLHAVAEDIGANGRISPAH
                     LGELNHILRHGLHYHQLRHMPDGARYSVAQVGDRLDQARATIASSLAQFLADGAAHRL
                     RICANQGCREVFIDRSPTGRRRWCDMRTCGNQAKVARHRARARQMETSTAAE"
     gene            complement(726..1712)
                     /locus_tag="VHK28_08625"
     CDS             complement(726..1712)
                     /locus_tag="VHK28_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2142317.1"
                     /translation="MAERRTTTRPRSGRRASVLDERAPAPLATTNGSRGVPDRVEVRK
                     TYKLYIGGAFPRTESGRAYQVLDARGRLLANASRGTRKDIRDAVRAARKAYPGWAART
                     AYNRSQILYRIAELMEGRREQFIAEVTAAEGVGRRRATRLVDAAIDRWVWYAGWADKF
                     SQAMGGVNPVAGSYFNFSIPEPTGVVGVIAPESSSLLGLVSRLAPAIVSGNAAVVLAS
                     ESRPLPAVTLTEVLATSDVPGGVVNLITGLRRELVQHLAGHMDVNALDAYGLDPSEAA
                     AIEEIATENVKRVVRPPSGGEASVDWLSPRAQSPYLIAEFVETKTVWHPIGS"
     gene            complement(1712..3241)
                     /locus_tag="VHK28_08630"
     CDS             complement(1712..3241)
                     /locus_tag="VHK28_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX2142318.1"
                     /translation="MTLTKKPDTRPAPASWGLAGGGKVPWEYAAAPEATDHITIQREY
                     GLFIDNEFVPARPRATFDVINPATEERLAKVARASKADVNRAVTAARRAYRTTWSRLP
                     GSERAKYLYRIARILQERSREFAVVESMNSGKPIKESRDVDVPLAAAHFFYYAGWADK
                     LEYAFPNRDPRPLGVAAQIIPWNFPLLMLAWKIAPALACGNTVVLKPASTTPLTAYLF
                     ADVVRQAELPPGVVNIITGPGEIGMELVKHPEVNKVAFTGSTAVGKMIARAVAGTHKK
                     VTLELGGKAANIVFDDAPLDQAIEGIINGIYFNQGEVCCAGSRLFVQESVYEPLLDKL
                     KARLSTLRVGDPLDKNTDVGAINSAGQLAKVKELVQAGIEEGAEIFQPDCELPDRGYF
                     FRPTLFTGVSQSHRIAQEEIFGPVLSVLTFRTPDEAVEKANNTPYGLSAGVWTEKGSR
                     IFKMVSAMRAGVVWANTFNRFDPTSPFGGYKESGYGREGGLHGLAAYLDLSDGNGTRA
                     R"
     gene            3452..>3882
                     /locus_tag="VHK28_08635"
     CDS             3452..>3882
                     /locus_tag="VHK28_08635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX2142319.1"
                     /translation="MTDAAHPLLEHALRFKVRTYELDQNGHVNNAVYLAWTENLAAEH
                     AEALGFGRAWSGEQGGGWVVRHHEITYHVAAVAGDEVDAVVRVEALGGVRGTRRTWFR
                     RAADAAQCLDAHHGVHLVTGYGGDVVRDLVMPHDPAAALLPA"
BASE COUNT          583 a         1373 c         1316 g          610 t
ORIGIN      
        1 accattcccg gacttcttat cagttacgtc cacagtgtac ccaaggctca gtaacttgcc
       61 aacgattgta tactggttac ctcatgtccc tgattcacga gatcccgccc gagccacagc
      121 ggtccgacga gctgttcgtg gccttcgcca acacgctcca tcacgagcgc ggcgcccccg
      181 tggacgacat cggcgacctg gacgcgctgc tggcctggct gcgcgaccac cacctgctga
      241 gtggcagggc acgcgccacc gaaacggcac ggctccatcg tgatccaggg gatgcaggaa
      301 ggcgcctgca gcgcttccgg cgcctgcgcg acgtcctgca cgcggtggcc gaggacatcg
      361 gcgccaacgg caggatcagc ccggcccacc ttggcgagct gaaccacatc ctgcgccacg
      421 ggctccacta ccaccagctc cgccacatgc cggatggtgc tcgctattcg gtcgcgcagg
      481 tgggcgaccg cctggaccag gcgcgggcca ccatcgccag ctcgctcgcc cagtttctgg
      541 ccgatggcgc cgcccatcgg ctgcgcatct gtgccaacca gggctgccgc gaggtattca
      601 tcgaccgctc accgaccggc aggcggcgct ggtgcgacat gcgaacctgc ggcaaccagg
      661 caaaggtcgc gcgacatcgg gcacgcgcac gccagatgga gacgagtacg gcggccgaat
      721 gaacctcagg agccgatggg gtgccagacc gtcttggtct cgacgaactc cgcgatcagg
      781 tagggcgact gcgccctggg gctgagccaa tcgaccgacg cctcgccgcc cgacggcggt
      841 cgcaccaccc gcttcacgtt ctcggtggcg atctcctcga tggcggccgc ctccgacgga
      901 tcgaggccgt aggcgtccag cgcgtttacg tccatgtgcc cggccaggtg ctgcaccagc
      961 tcgcggcgca ggccggtgat caggttgacc acgccgccgg ggacgtcgga tgtggcgagc
     1021 acctcggtca gggtcacggc cggcaggggc cgcgactctg aagccaggac gaccgccgcg
     1081 ttgcccgaga cgatggccgg ggccagccgg ctcaccagcc ccagcaggct tgacgactct
     1141 ggcgcgatga cgcccaccac gccggtcggc tccgggatgc tgaagttgaa gtacgagccg
     1201 gccaccgggt tcacgccgcc cattgcctgg ctgaacttgt cggcccagcc ggcgtaccac
     1261 acccatctgt cgatggccgc atccaccagc cgagtggcac gacgccgtcc gacgccctcc
     1321 gccgccgtca cctcggcgat gaactgctcg cgccgcccct ccatcagctc cgcgatgcgg
     1381 tacaggatct ggctgcggtt gtacgccgtg cgagccgccc aaccgggata ggccttgcgc
     1441 gccgcacgca ccgcgtcacg gatgtccttg cgggtccccc ggctggcgtt tgccagcagc
     1501 cgccctcgcg cgtcgagcac ctggtaggca cggccggatt ccgtgcgcgg gaaggcaccc
     1561 ccgatgtaca gcttgtaggt cttgcgcacc tccacccggt cgggcacgcc gcggctgcca
     1621 ttcgtggtcg ccaggggtgc gggagcacgc tcgtccagga ccgatgcccg ccgcccggag
     1681 cgagggcgcg tggtcgtgcg tcgctccgcc atcagcgcgc ccgcgtcccg ttgccgtccg
     1741 acaggtcgag gtaggcggcc aggccatgca gcccgccctc gcggccgtag ccggactcct
     1801 tgtagccgcc gaagggcgag gtcgggtcga agcggttgaa ggtgttggcc cacaccaccc
     1861 cggcgcgcat ggcactcacc atcttgaaga tgcggcttcc cttctcggtc cacacccccg
     1921 ccgacagccc gtacggcgtg ttgttcgcct tctcgacggc ctcgtccggc gtgcggaagg
     1981 tcaggaccga tagcaccggc ccgaagatct cctcctgcgc gatgcgatgg ctctggctga
     2041 cgccggtgaa cagcgtgggc cggaagaagt agccgcggtc gggcagctcg cagtctggct
     2101 ggaagatctc ggcgccctcc tcgatgccgg cctgcacgag ctccttgacc ttggccagct
     2161 ggccggcgga gttgatggcc ccgacgtcgg tgttcttgtc cagcgggtca cccacgcgca
     2221 gcgtggagag gcgtgccttc agcttgtcca gcagcggctc gtagacgctc tcctgcacga
     2281 agagccgcga gccggcgcag cacacctcgc cctggttgaa gtagatgccg ttgatgatgc
     2341 cctcgatggc ctggtccagt ggggcgtcgt cgaacacgat gtttgccgcc ttgccaccga
     2401 gctcgagcgt caccttcttg tgcgtcccgg ccacggcgcg ggcgatcatc ttgccgacgg
     2461 ccgtggagcc ggtgaaggcg accttgttga cctcgggatg cttgaccagc tccatgccga
     2521 tctcaccggg gccggtgatg atgttgacca cccccggcgg cagctcggcc tggcgcacca
     2581 cgtcggcgaa caggtacgcg gtcagcggcg tggtggaggc cggcttgagc accaccgtgt
     2641 tgccgcaggc cagggccgga gcgatcttcc aggccagcat gagcagcggg aagttccacg
     2701 ggatgatctg cgccgccacg cccagcggcc gcgggtcacg gttggggaag gcgtactcca
     2761 gcttgtcggc ccagccggcg tagtagaaga agtgggccgc ggccagcggc acgtcgacgt
     2821 cgcgcgactc cttgatcggc ttgcccgagt tcatcgactc gaccaccgcg aactcgcggc
     2881 tccgctcctg gaggatgcgc gcgatgcggt acaggtactt cgcccgctcg ctgccaggca
     2941 gtcgcgacca ggtggtccgg taggctcgac gggctgccgt tacggcgcga ttgacatcgg
     3001 ccttggatgc gcgtgcgacc ttggccagcc gctcctcggt ggcggggttg atgacgtcga
     3061 aggttgcgcg cggccgggcc ggcacgaact cgttgtcgat gaacagcccg tactcgcgct
     3121 ggatggtgat gtggtcggtc gcctcgggcg cagcggcgta ctcccagggg accttgccgc
     3181 ccccggccag tccccaggat gccggcgcgg ggcgggtgtc gggcttcttg gtgagggtca
     3241 tcgtggaccg attatcgcac cgtccgtcgt gccggccctc acgtcgtcgc cgaaccggac
     3301 ggattcacac caggtgacca tcagcgtcgc agagccgcgt ccgaaggcgc cggattcacg
     3361 tcgggcgtga aatcgagcgc tccgacaggc caatcagctg ccgggtgtgc gtatggcgtg
     3421 aattccggcc gtgcggccct acactcccct gatgaccgat gcggcccacc ccctgctcga
     3481 gcacgcgctg cggttcaagg tgcgcaccta tgagctcgac cagaacgggc atgtgaacaa
     3541 cgccgtgtac ctggcctgga cggagaacct cgcagccgag cacgccgagg cactcggctt
     3601 cggccgggcc tggagcgggg agcagggcgg cggctgggtc gtgcggcatc acgagatcac
     3661 gtaccacgtc gccgccgtag ccggagacga ggtggacgcc gtggtgcgcg tcgaggcact
     3721 gggcggcgtg cggggcacgc gacgcacctg gttccgccgg gcggccgacg ccgcccagtg
     3781 cctcgacgcg caccacggcg tccacctcgt caccggctac ggcggcgacg tggtacgtga
     3841 tctcgtgatg ccgcacgacc cagccgccgc cctgctcccc gc
//
LOCUS       DATACA010000421         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_779776, whole genome shotgun sequence.
ACCESSION   DATACA010000421 DATACA010000000
VERSION     DATACA010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779776"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="VHK28_08640"
     CDS             <1..1796
                     /locus_tag="VHK28_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2142320.1"
                     /translation="MVLETSWSTRTGWIIVRDVMLIGPWHSEERRTHTHKRAPTDYDA
                     EHILLRSVRCVNGSVQLVMECEPRFDYGRSFGEWSYTEDGYRQGRCRCPDTDVELTLT
                     GDLNLGFEGPKAIARTLMKEGETRFVALSWGGVNPPRTGDDAYGRLVWTAHHWQHWLA
                     RGHFPDHPWRSYLERSALTLKGLTYAPTGAVAAAPTTSLPETPGGERNWDYRYSWIRD
                     STFALWGLYTLGFDWEATDFIFFIAEVARADKNLQIMYGIGGERELPERTLDHLAGYE
                     GARPVRVGNAAYAQRQHDMWGALLDSVYLHTKSRDHLDESIWPIIMEQVETALEHWRE
                     PDRGIWEVRGEPKHFTSSKLMCWVALDRGARLARLRDEHDLADRWQKGADEIHADICA
                     NGVDDRGVFTQHYDTTALDASLLLMPLVRFLPPDDERIRRTVIAIADELSHDGLVLRY
                     KVEETDDGLSGREGAFAICSFWLVSAMVEIGELDRARRLCERLLALSSPLQLYGEEID
                     PETGRHLGNFPQAFTHLALINAVMHIIHAEQAGTGPLSPRSDEQRSPLTGEPVPPATS
                     EPPLSTPQPIGGGVGVGAAAQPAAGRDAEEE"
BASE COUNT          345 a          754 c          758 g          340 t
ORIGIN      
        1 ccatggtcct cgagaccagc tggagcactc ggacggggtg gatcatcgtc cgcgacgtga
       61 tgctgatcgg tccatggcac agcgaggagc gtcgcacgca cacccacaag cgagcgccca
      121 ccgactacga cgcggagcac atcctgctgc ggagcgtgcg ctgcgtgaac ggatccgtgc
      181 agctggttat ggagtgcgag ccgcgcttcg attacgggcg ttccttcggg gagtggtcgt
      241 acaccgagga cggctaccgg caggggcgct gccgctgccc cgacaccgac gtggagctga
      301 ccctgaccgg tgacctcaac ctcgggttcg agggtcccaa ggccatagcc cgtaccctga
      361 tgaaggaggg cgagacccgc ttcgtggccc tctcgtgggg tggcgtcaat ccgccgcgca
      421 cgggggatga tgcctacggt cgcctggtgt ggaccgcgca ccactggcag cactggctgg
      481 cgcgcggcca cttcccggac catccgtggc gcagctacct ggagcgctcg gccctgaccc
      541 tgaagggcct gacctatgcc ccgaccggcg cggtggcggc ggcgcccacg acttcccttc
      601 ccgagacccc cggcggcgag cgcaactggg actaccgcta ctcctggatc cgcgactcca
      661 ccttcgcgct gtggggcctg tacacgctgg gcttcgactg ggaggcgacc gacttcatct
      721 tcttcatcgc cgaggtggcg cgcgccgaca agaacctgca gatcatgtat ggcatcggtg
      781 gcgagcgcga gctgccagag cggaccctgg atcacctggc cggttacgag ggagcgcggc
      841 cggtgcgggt cgggaacgcg gcgtacgcgc agcgacagca cgacatgtgg ggcgcgctcc
      901 tggactcggt gtacctgcac accaagtcgc gcgaccacct ggacgagtcc atctggccga
      961 tcatcatgga gcaggtcgag acggccctgg agcactggcg cgagccggac cgcggcatat
     1021 gggaggtccg cggcgagccg aagcacttca cctcgtccaa gctgatgtgc tgggtcgccc
     1081 tcgaccgcgg cgcacggctc gcccggctgc gcgacgagca cgacctcgcc gaccgatggc
     1141 agaaaggcgc cgacgagatc cacgccgaca tctgcgccaa tggcgtggac gatcggggcg
     1201 tcttcaccca gcactacgac acgaccgccc tggacgcgtc cctgctgctc atgccgctgg
     1261 tgcgcttcct gccgcccgat gacgagcgca tacgccggac ggtgatcgcc attgccgacg
     1321 agctctccca cgatgggctg gtcctgcgct acaaggtgga ggagaccgat gacggcctct
     1381 ccggccggga gggtgccttc gccatctgct cgttctggtt ggtttcggcc atggtcgaga
     1441 tcggcgagct tgaccgtgcg cgccggctct gcgagcggct gctggcgctg tccagcccgc
     1501 tgcagcttta cggcgaggag atcgatccgg agacggggcg ccaccttggc aacttcccgc
     1561 aggccttcac gcatctcgcg ctgatcaacg ccgtgatgca catcattcac gcggagcagg
     1621 cagggaccgg cccgctctcg ccgcggagcg acgagcagcg cagcccgctg accggcgaac
     1681 cagtgccacc ggccaccagc gagccgccgc tgtccacgcc ccagccgatc ggcgggggag
     1741 tcggggtggg cgcggctgcc cagccggccg ccggtcgcga cgccgaggag gaatagcgcc
     1801 caggccttcg gcggtcccct ctgcgatgtc cacattgggg atggagtgtg gataactcag
     1861 ggagaggctt gaaaacccgg ggataagggg gttgcctcgt cgccgggccg gcgcctattc
     1921 tctggttgtc ccgaaggggc ggccaacggt tccggagatt gcatcaaagg gtccggaggc
     1981 ggttccttcg ggacatttcg ttgcctgctg ccgctcggcc tcctccacgg gcgacgtgcg
     2041 ccggcgccgt cagactccgg ttgtgccgca caggcgcaca gccgcacagg cgctcaaccg
     2101 acaccctgat ggcatcgggc gcgtcaagag caccctcgct ccggagctac ctttccccga
     2161 ggttgcgctg gaggcggcgc acgacctcgg cggtctg
//
LOCUS       DATACA010000422         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1101156, whole genome shotgun sequence.
ACCESSION   DATACA010000422 DATACA010000000
VERSION     DATACA010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101156"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VHK28_08645"
     CDS             complement(<1..366)
                     /locus_tag="VHK28_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142321.1"
                     /translation="MTPRPLDSAIAARLAGWRAERVAERLWARDGSLWAASGKAPEEV
                     ARWLGWLELPYAMRERVGELEHLARDVRADGYTRAAVLGMGGSSLAPELFSRVFGGLL
                     GAAPQGSGGMELRILDSTHP"
     gene            complement(363..1475)
                     /gene="tal"
                     /locus_tag="VHK28_08650"
     CDS             complement(363..1475)
                     /gene="tal"
                     /locus_tag="VHK28_08650"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX2142322.1"
                     /translation="MTDKNALQRLYAEHGQSPWIDFIDRDLITSGRLAGLVDDGIRGL
                     TSNPTIFAKAVAGGEYNDLIRREIDAGDDNRTIYEEIAVGDVGDAADILRGIYDASDG
                     TDGFVSIEVEPDLADDTKATLERARHLWGRLERPNVFVKIPATPAGLPAIEDAVAEGI
                     NINITLMFSVDVYRDVARAYIAGLRRRKERGEPIDRVASVASFFVSRVDTKVDKALDA
                     VGTDAARALRGQAAIANARQAYAAYGEIFGGTEFAGLRAAGARVQRCLWASTSTKNPD
                     YRDVLYVEELIGPDTVNTMPLETIQAFLDHGRVERTLDSDLRGADAIIARIEEQGISM
                     RQVTDELITEGVAAFAKSFDELLEAIETQRKALATA"
     gene            complement(1495..>2748)
                     /locus_tag="VHK28_08655"
     CDS             complement(1495..>2748)
                     /locus_tag="VHK28_08655"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX2142323.1"
                     /translation="GWHTVEIDGHDVEAIDRAYAEAAGIEDWPTAIIARTEKGHGVEA
                     VANQNGAHGKPVSDEAAAIEELGGPRQLNISVTPPEATGAPHRFPTQGELKLPSYEPG
                     TKEATRKAYGDALLALGRARGDVVAIDGEVGNSTYSEIFAKELPERFVQAYIAEQMML
                     SYAVGFQVRGWRPFAATFAAFLSRAYDFVRMAAVSRANIVLAGSHAGVSIGEDGPSQM
                     ALEDLASLRAVHGSTVLYPSDANQAAGLLAQMADRTGIVFMRTTREKTPVIYQPGERF
                     PIGGSRVVRKTDDDQVTLVGAGITLHEAMKAADALASDGISARVIDLYSVKPVDAETL
                     RAAASQTACMITVEDHYPEGGIGDAVLDALAGDGLGVPVRKLAVRIMPGSAKPEEQLA
                     AAGIDAKHIADAARELVRTRAAART"
BASE COUNT          363 a          992 c          936 g          457 t
ORIGIN      
        1 cgggtgtgtc gagtccagga tgcgcagctc catcccgccc gacccctgcg gcgctgcgcc
       61 gagaagcccg ccgaagacgc ggctgaacag ctcgggcgcc aggctggagc cgcccatgcc
      121 cagcaccgcg gcacgggtgt aaccgtccgc tcgcacatca cgggccaggt gctcgagctc
      181 gccgacgcgc tcccgcatgg cgtacggcag ctccagccac cccagccagc gagcgacctc
      241 ctccggcgcc ttgcccgacg ctgcccacag actgccgtcg cgcgcccaca gccgctccgc
      301 gacccgctcc gccctccagc cggcgagccg cgccgcgatg gccgagtcga gcgggcgtgg
      361 ggtcatgcgg tggcgagcgc cttgcgctgg gtctcgatcg cctcgagcag ctcgtcgaag
      421 gacttcgcga aggcggcaac gccctcggtg atcagctcat cggtcacctg ccgcatgctg
      481 atcccctgct cctcgatgcg ggcgatgatc gcgtcggcgc cgcgcaggtc gctatccagc
      541 gtgcgctcca cccgtccgtg gtcgaggaag gcctggatgg tctccagcgg catggtgttg
      601 acggtgtccg ggccgatgag ctcctcgacg tacagcacgt ctcggtagtc cgggttcttg
      661 gtcgaggtgc tggcccacag gcagcgctgc acgcgcgctc cggccgccct caggccggcg
      721 aactcggtcc cgccgaagat ctcgccgtag gcggcgtagg cctggcgcgc gttggcgatg
      781 gccgcctgcc cgcgcagggc acgggcagca tcggtcccga cggcgtcgag cgccttgtcc
      841 accttggtat ccacccggct gacgaagaag ctggcgaccg atgcgacccg gtcgatcggt
      901 tcgccacgct ccttccgccg ccgaaggccg gcgatgtagg cgcgggccac gtcgcggtag
      961 acgtccacac tgaacatgag ggtgatgttg atgttgattc cctcggctac cgcatcctcg
     1021 atggccggca gccccgccgg cgtggccggg atcttgacga agacgtttgg ccgctcgagc
     1081 cgcccccata ggtgacgcgc ccgctccagg gtggccttcg tgtcgtccgc caggtccggc
     1141 tcgacctcga tggagacgaa gccatcggtc ccgtcggagg cgtcgtagat tccgcgcagg
     1201 atgtcggccg cgtcgccgac atcaccgacc gcgatctcct cgtagatggt gcggttatcg
     1261 tcaccggcgt cgatctcgcg gcggatgaga tcgttgtact cgccgccggc caccgccttg
     1321 gcgaagatgg tcgggttgct ggtcaggccg cgaatgccgt cgtccaccag cccggccagc
     1381 cggccggagg tgatcaggtc acggtcgatg aagtcgatcc acgggctctg cccatgctcc
     1441 gcgtacaggc gctgcagggc gttcttgtcg gtcacttggc tctcctcggg tcgttcaggt
     1501 gcgggcagcg gcccgcgtgc ggaccagctc gcgcgccgca tccgcgatgt gcttggcgtc
     1561 gatgccggcg gcggccagct gctcctccgg cttcgccgag ccgggcatga tgcgcaccgc
     1621 cagcttgcgc accggcacgc ccagcccgtc gccggccagg gcgtcgagca ccgcgtcgcc
     1681 gatgccgccc tccggatagt gatcctcgac ggtgatcatg catgccgtct ggctcgcggc
     1741 ggcgcgcagc gtctcggcgt ccaccggctt caccgagtac aggtcaatga cccgggcact
     1801 gattccatcc gaggcgagcg cgtccgccgc cttcatggcc tcgtgcaggg tgatgcccgc
     1861 gccgaccagg gtgacctggt cgtcatcggt cttgcgcacc acgcggctgc ctcctatggg
     1921 gaagcgctcc ccgggctggt agatgaccgg cgtcttttcg cgggtggtgc gcatgaagac
     1981 gatgccggtg cggtccgcca tctgcgccag caggccggcc gcctggttgg catccgacgg
     2041 gtagagcacc gtgctgccgt gcaccgcgcg caggctggca aggtcctcca gcgccatctg
     2101 cgacggtccg tcctcaccga tgctgacgcc ggcgtgcgac ccggccagga cgatgttggc
     2161 gcggctcacc gccgccatgc gcacgaagtc gtaggcgcgt gacaggaagg cggcaaaggt
     2221 ggcggcgaac ggacgccagc cccgcacctg gaagcccacg gcgtagctga gcatcatctg
     2281 ttcggcgatg taggcctgca cgaagcgctc gggcagctcc ttggcgaaga tctccgagta
     2341 cgtcgagttg cccacctcgc cgtcgatggc caccacgtcg ccgcgcgccc gtcccagcgc
     2401 cagcagcgcg tcgccgtacg ccttgcgcgt cgcctccttg gtgcccggct cataggacgg
     2461 cagctttagc tccccctggg tggggaaccg atgcggcgct cccgtggcct cgggcggggt
     2521 cacggagatg ttcagctggc gcgggccccc cagctcttcg atcgcggctg cctcgtcgct
     2581 caccggtttg ccgtgcgccc cgttctggtt ggccacggcc tccacgccat ggcccttctc
     2641 ggtgcgagcg atgattgctg tcggccaatc ctcaattcct gcggcttcgg cataggcgcg
     2701 gtcgatggct tcgacgtcgt ggccgtcgat ctcgacggtg tgccagcc
//
LOCUS       DATACA010000423         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2627148, whole genome shotgun sequence.
ACCESSION   DATACA010000423 DATACA010000000
VERSION     DATACA010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627148"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHK28_08660"
     CDS             <1..627
                     /locus_tag="VHK28_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX2142324.1"
                     /translation="TADILRAALRVVGTADGSDCVSSSFLMHLQDGRTFGYGDCAVLP
                     EPDAGQLAEVAIATSRTFAALTGEEPQVAMLSFSTMGSADHASVTRVRDATAIVRERE
                     PGLAVDGELQFDAALLESVARSKAPDSGVAGHANVFIFPNLAAGNIGYKITQRLGGAA
                     AYGPILQGLALPVNDLSRGCTVADIVNVAIISVLQSQTSVTRLGPSGL"
     gene            670..>2082
                     /locus_tag="VHK28_08665"
     CDS             670..>2082
                     /locus_tag="VHK28_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007272617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX2142325.1"
                     /translation="MKATTDRTGNVAGPVASAGPAATPGPDQPERLAKTLKPSWVWAI
                     ALGSAVGWGAFILPTDWIAAAGPVGAMSGFLIGGGLMVLIAVSYGFLIRSFPVSGGEL
                     AFALVGFGRTHAFFCGWFLTLGYTCIVALNASALALLFRKLLPGVVQQGYLYTIAGFD
                     VYIVEVLISITALAVFAYLNIRGTALSGRVQFIACVIMLIAVACILTAVLIHPSSLVS
                     NAAPAFPEGVSPLTAILAIVAIAPWAYIGFDNVPQAAEEFDFPPSKAMKLIVLALLAA
                     AVLYAAMIAAVAVAEPWEQLVSGQSAWGTADAVTGLLGGGGMLLLAIGITMGVTTGLN
                     GFYVSASRILLAMGRAQMIPQVFAKLHPKHKTPYVGILFVGAFCLATPWFGRAALEWV
                     VNMSAVGVTVAYLYTCLCAFKLFRSSGSAPRPGDLEGTRSTTKKVLSMVGAVISVAFM
                     ALLLFPGSPAVLGPESMTALL"
BASE COUNT          295 a          747 c          668 g          372 t
ORIGIN      
        1 accgccgata tactgcgcgc ggcgttgcgc gtggtcggca cggcggacgg ttcggactgc
       61 gtgagcagca gcttcctgat gcacctgcag gacggccgga ccttcggcta cggcgactgc
      121 gccgtgctgc cggagcccga cgctgggcag ctcgccgagg tggcgatcgc caccagccgc
      181 accttcgcgg cactgaccgg cgaggaaccg caggtggcca tgctctcctt cagcaccatg
      241 ggcagcgccg accatgccag cgtgacccgg gtccgcgacg ccaccgccat cgtacgggag
      301 cgcgagccgg gcctggcggt cgacggcgaa ctgcagttcg acgccgccct gctcgaatcc
      361 gtggcccgca gcaaggcgcc ggactccggc gtcgccggcc acgccaatgt gttcatcttc
      421 cccaatcttg ccgcgggcaa catcggctac aagatcaccc agcggctcgg cggagcggcg
      481 gcctacgggc cgatcctgca gggactcgcc ctgcccgtca acgacctgtc ccgcggctgc
      541 accgtggcgg acatcgtcaa cgtagcgatt atcagcgttt tgcaatccca aaccagcgtc
      601 accaggctcg gtccctccgg actgtagcca gcacgggcag agactctgtc cctggagaat
      661 gaggttgaca tgaaagcaac aactgatcgg accggcaacg tggccggccc ggtagcatcg
      721 gccggaccgg cggcaacccc cggacccgac cagcctgaac ggttggccaa gaccctcaag
      781 cccagctggg tctgggccat cgccctcggc tcggccgtcg gctggggcgc gttcatcctg
      841 cccaccgact ggatcgccgc ggcgggcccg gtgggcgcga tgagcggttt cctgatcggc
      901 ggcggactga tggtgctgat cgccgtcagc tacggcttcc tgatccgcag cttccccgtt
      961 tccggcggtg agctggcgtt cgcgctggtg ggcttcggcc ggacccacgc cttcttctgc
     1021 ggctggttcc tgaccctggg ctacacctgc atcgtggctc tgaacgcctc ggccctggcc
     1081 ctgctgttca ggaaactgct gccgggcgtg gtccagcagg gctacctcta caccattgcc
     1141 ggtttcgacg tgtacatcgt ggaagtgctt atctcgatca ccgcgctggc ggtcttcgcc
     1201 tacctgaaca tccgcggcac ggcactgtcc ggccgggtcc agttcatcgc ctgcgtgatc
     1261 atgctgatcg ccgtcgcctg catcctgacc gccgtgctga tccaccccag cagcctggtc
     1321 agcaacgccg cgccggcctt ccccgagggc gtctcgccgc tgaccgccat cctcgcgatt
     1381 gtcgccattg ccccttgggc ctacatcggg ttcgacaacg tgccgcaggc cgccgaggaa
     1441 ttcgacttcc cgccgagcaa agccatgaag ctgatcgtgc tggcgctgct cgccgcggcc
     1501 gtgctgtacg cggccatgat tgccgccgtc gctgttgccg agccgtggga acagctggtt
     1561 tccggccaat cggcctgggg cacggctgac gcggtcaccg gtctgctcgg cggtggcggc
     1621 atgctgctgc tggccatcgg catcaccatg ggtgtgacca ccggcctgaa cggattctac
     1681 gtctccgcca gccgcatcct gctggccatg ggccgggccc agatgatccc gcaggtcttc
     1741 gccaagctgc acccgaagca caagacgccg tacgtgggca tcctgtttgt cggcgccttc
     1801 tgcctggcga ccccgtggtt cggccgggcc gcgctggaat gggtggtcaa catgtccgcc
     1861 gtcggcgtta ccgtggccta cctgtacacc tgcctgtgcg ccttcaagct cttccgcagc
     1921 tccggctccg caccccgccc cggcgatttg gaaggtacgc gttcgacgac gaagaaggtg
     1981 ctcagcatgg tcggcgcggt catctcggtg gccttcatgg cgctgctgct gttccccggc
     2041 tcgcccgccg tgctgggccc ggagtccatg acagccctgc tg
//
LOCUS       DATACA010000424         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1824878, whole genome shotgun sequence.
ACCESSION   DATACA010000424 DATACA010000000
VERSION     DATACA010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824878"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1543)
                     /locus_tag="VHK28_08670"
                     /pseudo
     CDS             complement(206..1543)
                     /locus_tag="VHK28_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820019.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            complement(1674..2446)
                     /locus_tag="VHK28_08675"
     CDS             complement(join(1674..2119,2119..2446))
                     /locus_tag="VHK28_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611628.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2142326.1"
                     /translation="MGLTDEQWAVLAPLIEQCRPPAKVPPRHLRRTIGAIIWRHDNGA
                     KWRALPTELGPWWMAAQTFIRWTRLGVWERLLSLVQERGVQLGMTFLDGTNVRAHQKA
                     AGAARKGNTSAERDQREALGRSRGGHGTKACVIADGQGRAIAFRLAPGQAHELPHAVP
                     LLDHLPRVPKWVVADRGYASYAFRQHVWDLGARPAIPPKRNEEPVACPDYIYTNRNLV
                     ERLWARLKEWRAVATRYEKTACSFMGVLCMAAAMDWLKS"
     gene            complement(2492..3100)
                     /locus_tag="VHK28_08680"
     CDS             complement(2492..3100)
                     /locus_tag="VHK28_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2142327.1"
                     /translation="MARGRAGWALGFEDEVWFSRLAQPALHAWRPQGERLRLIMHAAD
                     RDDPEAKALACHGLWLPGPERMLLRFTAGRPVSAMTCTFLAWAAERLAAEGVRVLALI
                     WDNAPWHVSREVCTWIRNHNRQVKAAGAGCRLLVCRLPSRSPWLNPIEPRWAHGKRAV
                     VEPERKLTSEELQQRLCRHYCCQLLPRSHRRWIEAALGPVSA"
BASE COUNT          472 a         1099 c         1159 g          482 t
ORIGIN      
        1 cgagcgttgt cttcaagatt gctccgaagg ctgatggcgg cggtggtcct gaacccggaa
       61 cctagccggg ataattcacc ggcgccgcgg cacgggcgga tcagcggtgg cggcggtcgc
      121 ggagaacggt ggcgccgggg gcgcggtcgt ccggggctgc tcggttacag gggaggggct
      181 cgtccggggc acgccgcccc gcgggtcaag gcggccttgc cgcccgggcg gcgagcaggg
      241 tgaggctgcc gcggtagggg tagctggagg gcagcgcgag cttgatccgg gtcgccagct
      301 cggtgacccg accggccacc ttgaccagca tgagacggat cgtgtcgaac tgggcctcgc
      361 gccagaacga ccgcttcggc gccaggccgc gcaaggtgtg caggagccag taggcggcgg
      421 tgtgcagcac gaggcggaac tggttggccg tggccttggt gcacgacgtc cggtccgagg
      481 cgaggtggcg cttgtgcgcc ttgatcaggt tttcggcctg gccgcgggcg cagtagacat
      541 cctcgtagag ccagcggggc accccggcga ggttggtgac gatgaaacgg gtgtcgctgc
      601 cccggtccga ggcctccact cgggcgatca cccggcgcgc ggtgctccag gtcctggccg
      661 cgtagaggaa ctcatcgtag cggcggacct tcgtgccttc gccctcaacc cggcgcacgg
      721 cggcttcctc ggccaggccc gcggcgcggg cgagcaggac cttgttgccg gcgagcccga
      781 aggcgtaggc gacccggttg cgctcgcacc aggccatcgc ctcatgacgg gcttaatgac
      841 tgtcgccgcg gaccaggatg tcgacacggg gccagcgctt gcggatggcg ccgatcacgt
      901 ggcgcaggat gagggtcacc tcggccccgt ccggggtctt gcccggccgc aggatcaccg
      961 ccaccggctt gccggtggtg gcctcgtgga cgtggatcgg caggaagcac cgctcgtcgt
     1021 gataggcgtg gaacagcgag agctgctggc ggccgtgcgc cggatcgcag gtgtcgtcga
     1081 tgtcgagcac ggtccggcgc ggcacctcgg tgaagctgtc gcagaacagc tcgaccatgg
     1141 ctgccatcat ccgcttgagc gcggcagcgc cgggcaggtt ctccagccgg cacatggtcg
     1201 gctgcgagca cagatcctgc ccgctctcgg gcaaccggcc gaccgccatc ttgaaggccg
     1261 ggtcgatccg cagcgcgtcg cagtcgttgg cgtcggggta gccggccgcg atcagcagca
     1321 cgcgaaagcg gatcatctcg gccagcctat ggcgcacccg ctccggtgct ctgaggtcgg
     1381 tgaggcagcg cgccaggcgc tccgcaagct cgagccgccg ctcgacctcg gccaggagca
     1441 gcaccccggc atccgaggtc agccggccac catcgaaggc gacatggacc ggcttgccgc
     1501 aaaccggtga caggccgggc aggaaggggg cattctcagc catggcgggc gcggcactcc
     1561 gtcgagccgt ggtgtctctg cgctagcacc agaaacatac acgatctcag acgtttacgc
     1621 cgcgtccgcc agcccgtcgc tcaggcctgg tgaattattc aggctaggcc ctgttagctc
     1681 ttgagccaat ccatggcggc ggccatgcag aggacgccca tgaaggagca ggcggtcttc
     1741 tcgtagcgag tggcgacggc gcgccactcc ttgagccggg cccagaggcg ctcgaccagg
     1801 ttgcggttgg tgtagatgta gtcggggcag gcgaccggtt cctcgttgcg cttgggcggg
     1861 atcgcgggcc gcgcgcccag gtcccagaca tgctgccgga aggcgtagct tgcgtagccg
     1921 cggtcggcca ccacccactt gggcacgcgc ggcaggtgat cgagcagcgg cacggcatgc
     1981 ggcagctcat gcgcctgccc cggtgcgagg cggaaggcga tggctcgtcc ctggccgtca
     2041 gcgatcacgc aggccttggt gccatggccg ccacgcgacc ggccaagcgc ctcacgctgg
     2101 tcgcgctccg ccgaagtgtc ccctttcgcg ccgcacccgc cgccttctgg tgcgcccgca
     2161 cgttggtgcc gtccaggaac gtcatgccga gttgcacgcc gcgctcctgc accaggctca
     2221 gcagccgctc ccagacaccc aggcgcgtcc agcggatgaa ggtctgggcc gccatccacc
     2281 atgggccgag ctccgtgggc agggctcgcc acttcgcacc attgtcgtgg cgccagatga
     2341 tcgcgccgat cgtgcgccgc aggtggcgcg gcggcacctt ggcaggcggg cggcactgct
     2401 cgatcagcgg cgccagcacc gcccattgct cgtccgtcag acccatcgcg ccctccccaa
     2461 aaccagaaga acgcccacgc ccgcctcgcg ttcaggcgct aacaggccct agtgcagctt
     2521 caatccacct tctgtgcgag cggggcagga gctggcagca gtaatggcgg catagccgct
     2581 gctggagctc ctcgctggtg agcttgcgct cgggctcgac cacggcccgc ttgccgtgcg
     2641 cccatcgggg ctcgatcggg ttcagccacg ggctcctgct cggcaggcgg cagaccagga
     2701 gacggcaccc ggcgcccgcc gccttgacct gccggttgtg gttcctgatc caggtgcaga
     2761 cctcgcggct gacgtgccag ggggcattgt cccagatcag cgccagaacc cggacgcctt
     2821 cagccgcgag gcgttcggca gcccaggcga ggaaggtgca ggtcatggcg ctgaccggcc
     2881 ggcctgcggt gaaccgcagc agcatccgtt ccggtcccgg aagccacagg ccatggcagg
     2941 cgagcgcctt ggcctcgggg tcatcgcggt cggccgcgtg catgatcagg cgcagccgtt
     3001 cgccttgcgg cctccaggcg tgcagggccg gttgtgccaa gcgggagaac cagacctcgt
     3061 cctcgaagcc cagagcccag cccgcccggc ctcgtgccaa gccgatcagc cggtcgcggc
     3121 ggcgtttttt tgcgcgtagg cgggatcggg gctggtgagc cagcgcttgg cccgcttcca
     3181 gccgacctcc aggcggagca gcgcctggcg ga
//
LOCUS       DATACA010000425         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2547262, whole genome shotgun sequence.
ACCESSION   DATACA010000425 DATACA010000000
VERSION     DATACA010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547262"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..903)
                     /locus_tag="VHK28_08685"
     CDS             complement(49..903)
                     /locus_tag="VHK28_08685"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142328.1"
                     /translation="MLRRYRWLTPYLFLLPGGLWLLAFFVVPLAVMAAVSLQTGSLGQ
                     GYQLTWNFGVYPEMIGQYSGFFLRSVGYALAVTALCLLIGFPLAYTIAFRGGRAKSIL
                     LLLVILPFFTSFIIRTLAWKLILQDNGFVLGTFKEIGILDAGFRVLATPISVISGLTY
                     NFLPFMVLPLYVALEKIDPRLIEAATDLYASRWQAFRRVTLPLAMPGIFAGSLLVFIP
                     AVGDYVNALILGSPQTQMIGNVIQRLFLNTNAYPEASALGFLMMAAVLVMITAYARMV
                     GSEELMDR"
     gene            complement(937..2217)
                     /locus_tag="VHK28_08690"
     CDS             complement(937..2217)
                     /locus_tag="VHK28_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX2142329.1"
                     /translation="MSEDQRTQLERAIARAFLPQRRLSRRVFLRQAGRGGVAAGAALS
                     LPALLAACGIGAQSQAPSAAPSIGSEPAGTLDWANWPAYIDIDEETGDYPTIQAFEEA
                     NGITVNYTEAVNDNEEFFGIIQPDLAAGRSPGYDLIVMTDWMIERLIRLGYVMPLDKS
                     KLTNWEANCLDLYRDPWYDPGNTHSVAYQSGLVGIGYDPNLTGREITTFDDLLDPEFS
                     GRVGMFSEMRDTMSLTLLSLGVVPQDATIEDARRAQEKLLEPARNGQFRNFYGNDYYD
                     ALAAGDLALTMAWSGDVSQMKLYDNDAVEFVVPDTGGTLWVDNMAIPNGAAHPVDAHL
                     MMDYLYTLDAAVPLTEYVGYYSPVEGVTERVLEDAETARAEGDDEWADALEVIAATAV
                     PSDDILDRVHTYKRLTEEEEREWNELFNEVVQGG"
BASE COUNT          383 a          777 c          836 g          384 t
ORIGIN      
        1 tggcgaccgg gccggccgcc ggggtgccga tggggacggc cggcgcgctc atcggtccat
       61 cagctcctcg ctaccgacca tgcgggcgta ggccgtgatc atgaccagca ccgcggccat
      121 catcaggaag cccagcgcgg atgcctcagg gtaggcgttg gtgttcagga agaggcgctg
      181 gatgacgttg ccgatcatct gcgtctgggg gcttcccagg atcagggcgt tgacgtagtc
      241 acccaccgcc gggatgaaga ccagcagcga gccggcgaag atgccgggca tggccagcgg
      301 cagcgtgacc cggcggaacg cctgccagcg gctggcatac aggtccgtgg ccgcctcgat
      361 cagtcgtgga tcgatcttct cgagcgccac gtacagcggc agcaccatga agggcaggaa
      421 gttgtaggtc agcccactga tgaccgatat cggcgtggcc agcacccgga agccggcgtc
      481 caggatgccg atctccttga aggtgcccag cacgaagccg ttgtcctgca ggatcagctt
      541 ccaggccagg gtgcggatga tgaagctggt gaagaacggc aggatgacca gcagcagcag
      601 gatgctcttg gcccggccgc cgcggaaggc gatggtgtag gccagcggga agccgatcag
      661 caggcacagc gcggtgacgg ccagcgcgta ccctaccgag cgcaggaaga agccggagta
      721 ctggccgatc atctcggggt agaccccgaa gttccaggtc agctggtagc cctgccccag
      781 cgagccggtc tgcaggctga ccgctgccat cacggccagc ggcaccacga agaaggcgag
      841 cagccacagc ccgccgggca gcaggaacag gtacggcgtc agccagcgat agcgtcgcag
      901 catggcggct tcagcgggga agcgccgccg gcgcggctaa cccccctgca ccacctcgtt
      961 gaagagctcg ttccactcgc gctcctcctc ctcggtgagg cgcttgtagg tgtggacgcg
     1021 gtccaaaatg tcgtcgctgg ggacggcggt ggcggcgatc acctccaggg catccgccca
     1081 ttcgtcgtcg ccttccgccc gcgccgtctc ggcatcctcc agcacgcgct cggtgacgcc
     1141 ctcgaccggg ctgtagtagc cgacgtactc ggtgagcggc acggccgcgt ccagggtgta
     1201 gaggtagtcc atcatcaggt gggcatcgac cgggtgcgcc gcaccgttcg ggatggccat
     1261 gttgtcgacc cacagcgtgc cgccggtgtc cggcaccacg aactcgaccg cgtcgttgtc
     1321 gtacagcttc atctgactca cgtcgccgga ccaggccatg gtcagcgcca ggtcgcccgc
     1381 ggccagcgcg tcgtagtagt cgttgccgta gaagttgcgg aactgtccgt tccgcgccgg
     1441 ctcgagcagc ttctcttggg cgcgccgcgc atcctcaatg gtggcgtcct gcggcaccac
     1501 gcccaggctg agcagcgtca gcgacatcgt gtcgcgcatc tcgctgaaca tccccacccg
     1561 tccggagaac tcggggtcga gcaggtcatc gaaggtcgtg atctcgcgac cggtcagatt
     1621 ggggtcgtag ccgatgccca ccaggccgga ctggtaggcg accgagtggg tgttgcctgg
     1681 gtcgtaccac gggtcgcggt acaggtccag gcagttggcc tcccagttgg tgagcttcga
     1741 cttgtcgagc ggcatcacgt agccgaggcg gatcaggcgc tcgatcatcc agtcggtcat
     1801 cacgatcagg tcgtatcccg gggagcggcc ggcggcgagg tcgggctgga tgatgccgaa
     1861 gaactcctcg ttgtcgttga ccgcctcggt gtagttgacg gtgatgccgt tcgcctcctc
     1921 gaaggcctgg atggtggggt agtcgccggt ctcctcgtcg atgtcgatgt aggcgggcca
     1981 gttcgcccag tcgagcgtgc ctgccggctc gctgccgatg ctgggtgccg ccgacggcgc
     2041 ctgcgactgg gcaccgatgc cgcacgccgc cagcagcgcc gggagggaaa gcgcggcgcc
     2101 ggccgcgacc ccacctcgtc cggcctggcg caggaacacg cggcgcgaga gccggcgctg
     2161 cggcaggaag gcgcgggcga tggcgcgctc cagttgggtt cgctggtcct cactcatcgg
     2221 gctctgactc ctcctcgctg tgcggatcgg cgtgaacgtg ctccgaggcc tcgatcacga
     2281 aagtgtgctg gggcttccag ctgacgcgga cggcctcgcc gtccgagagg gccgcggtcg
     2341 cgccgctgac ctccaggttc tgggcgtaga ccaccatgcg
//
LOCUS       DATACA010000426         1763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1101547, whole genome shotgun sequence.
ACCESSION   DATACA010000426 DATACA010000000
VERSION     DATACA010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101547"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VHK28_08695"
     CDS             complement(<1..906)
                     /locus_tag="VHK28_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2142330.1"
                     /translation="MVPAASDLLASLRAAVGDAHILTDAGLRAGYETDWTRRWRGDAL
                     AVVRPASTEQVAAVLLACTDAGAAAVPQGGNTGLVGGSVPRAASHRSQIILSTVRLRD
                     LEDVDVAAGEVTAGAGVTLAALQQHVRAAGYAFGVDLGARDSATIGGTIAANAGGIQV
                     LRHGPMRRQLVGIEGVLADGSVLRRLPGLVKDNTGYHLPSLLAGSEGTLAVVTRARLR
                     LVPHLPRRAAAMLALDGADEAADLAGVLRRRLPNLLAAELMFEDGIGLVVRHAGLSHP
                     FSRPHPAYLLVEVDGASDPTDELAAA"
     gene            970..>1763
                     /gene="moaA"
                     /locus_tag="VHK28_08700"
     CDS             970..>1763
                     /gene="moaA"
                     /locus_tag="VHK28_08700"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX2142331.1"
                     /translation="MSELPMLPRDTLNRPLHDLRISVTDRCNFRCVYCMPRAVFGPDH
                     AFLPRAELLSFEEIARLVAIFTRLGVEKVRLTGGEPLVRREVANLVRQLADIPGVRDL
                     TMTTNGALLPRHAVELKAAGLQRVTISLDADDDDTFTRMNDARVPVRVVLAGIQAAEE
                     AGLGPIKINMVVKRGWNEHAILPMARRFRGTGRILRFIEYMDVEHSNGWRLDDVVTAD
                     EILRTVSAEFPLEPMPPTHPGEVAERYRYADGSGEIGIIGSVSRPF"
BASE COUNT          260 a          641 c          626 g          236 t
ORIGIN      
        1 ggctgccgcc agctcatcgg tcgggtccga cgcgccgtcg acctccacca gcaggtacgc
       61 cgggtgcgga cgcgagaacg gatgcgacag tccggcgtgg cgcaccacca gcccgatccc
      121 gtcctcgaac atcagctcgg ctgccagcag gttcgggagc cggcggcgca gcaccccggc
      181 gaggtcggcc gcttcatcgg caccgtcgag ggccagcatg gcggcggccc gccgcggcag
      241 gtgcgggacg agtcgcagcc gcgcgcgggt gacgacggcc agcgtgccct ccgacccggc
      301 gaggagcgag ggcaggtgat agccggtgtt gtccttgacc agccccggca accggcggag
      361 cacgctgccg tcggccagca cgccctcgat gccgaccagc tgccggcgca tcggtccatg
      421 gcgcagcacc tggatgccac ccgcgtttgc ggcgatcgtg ccgccgatgg tggcgctgtc
      481 gcgtgcgccc aggtccacgc cgaaggcgta gcccgccgct ctgacgtgct gctgtagcgc
      541 cgccagcgtg acgccggcgc cggcggtcac ctccccggcg gccacatcca catcctccag
      601 gtcacggagg cgcaccgtgg acaggatgat ctgcgaccga tggctggcgg cgcgcggcac
      661 ggagccgccg acgagcccgg tgttgccgcc ctgcggcacg gcggccgccc ccgcatcggt
      721 gcaggccagg agcacggcgg ccacctgctc ggtgctcgcc ggcctcacca ccgcgagcgc
      781 atcgccgcgc cagcggcgcg tccagtccgt ctcgtagccg gcccgcaggc cggcatcggt
      841 caggatgtgt gcgtcgccga cagccgcacg cagggaagcc agcaggtccg aggcagcggg
      901 caccatcggt ccacatgctg acaggtgcgg atttggcagg cgagccgctg cctgtcacga
      961 tcggaggcga tgtccgagct tccgatgctg ccccgcgaca cgttgaacag gccgctgcac
     1021 gacctgcgca tcagcgtcac cgaccgctgc aacttccgct gcgtgtactg catgccgcgc
     1081 gcggtgttcg ggccggacca cgcattcctg ccccgcgcgg agctgctgag cttcgaggag
     1141 atcgcacgcc tggtggcgat cttcacgcgg ctgggcgtgg aaaaggtccg cctgaccggt
     1201 ggcgagccgc tggtgcgtcg cgaggtggcc aacctggttc ggcagctggc ggacatcccg
     1261 ggcgtgcggg acctgaccat gaccaccaac ggcgcgctgc tgccgcgcca tgccgtggag
     1321 ctgaaagcgg ccgggctcca gcgggtcacc atcagcctgg acgcagacga cgacgacacc
     1381 ttcacccgca tgaacgacgc tcgggtcccg gtgcgggtcg tgctggccgg catccaggcc
     1441 gccgaggagg ccggcctggg accgatcaag atcaacatgg tggtgaagcg cggctggaac
     1501 gagcacgcca tcctgcccat ggcccgccgc ttccggggca ctggtcgcat cctgcgcttc
     1561 atcgagtaca tggatgtcga gcactcgaac ggctggcgcc tggacgacgt ggtcaccgcg
     1621 gacgagatcc tgcgcaccgt ctcggccgag ttccccctgg aaccgatgcc gcccacgcac
     1681 cccggcgagg tcgccgagcg ctaccgctat gcggatggca gcggcgagat cgggatcatc
     1741 ggcagcgtga gccgcccgtt ctg
//
LOCUS       DATACA010000427         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2387026, whole genome shotgun sequence.
ACCESSION   DATACA010000427 DATACA010000000
VERSION     DATACA010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387026"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VHK28_08705"
     CDS             complement(<1..630)
                     /locus_tag="VHK28_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142332.1"
                     /translation="MRIELPRVVVHVLSGLAERGGDAVLVGGCVRDLVAGEPPGDWDV
                     ATSLPPESVAAAFPGSSWQNPFGTVTVSGQPDEPRVEVTTFRVEETYRDRRRPESVRW
                     GTSLEEDLARRDFTVNAMAWRPTDLDAGEGMLVDPHQGAADLERRVLRAVGDPDQRVA
                     EDALRLVRAVRFAVRYRLALDPATAAAIQRNAEAAASLSGERVRDELLRM"
     gene            complement(666..1199)
                     /locus_tag="VHK28_08710"
     CDS             complement(666..1199)
                     /locus_tag="VHK28_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX2142333.1"
                     /translation="MINAPVLVLNQNYEPLNVCDIRRAFRLLGASKAELLERNHQVIH
                     TPTIEIPAPSVIRLQYHIKRPRPRVKLSRREIFARDRHTCQYCGTVSRDLTIDHVVPK
                     HRGGRHEWENLVAACRSCNHRKGSKTLAEARMHLLREPRATRADVRYLFGSYLSDERN
                     DAWRSYLFLEPSPAEGS"
     gene            1625..2506
                     /gene="pdxS"
                     /locus_tag="VHK28_08715"
     CDS             1625..2506
                     /gene="pdxS"
                     /locus_tag="VHK28_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2142334.1"
                     /translation="MERSTWATKVGLARMLSGGVIMDVVTADHARIAEEAGAVAVMAL
                     ERVPSDIRKFGGVARMSDPKLIEEIKAAVSIPVMAKCRIGHIAEAQILQQLDVDFIDE
                     SEVLTPADEEHHIDKHQFKVPFVCGCRDLGEALRRIGEGAAMIRTKGEAGTGNIVEAV
                     RHMRAVMKGIRELSGMRPDERFAVAKELRAPLDVVQQTAELGELPVPNFSAGGVATPA
                     DASLMRQLGAQAVFVGSGIFKSENPERVAKAIVLATTHYDNPEILAEVSAGLGEPMHG
                     IELGEIPLEERLAVRGW"
     gene            2520..3179
                     /gene="pdxT"
                     /locus_tag="VHK28_08720"
     CDS             2520..3179
                     /gene="pdxT"
                     /locus_tag="VHK28_08720"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2142335.1"
                     /translation="MAQPAPSAPLPESGAELEPSPVGGPSRRRPRVGVLALQGAVREH
                     MDALREIGAEPVAVRLPHDLDGLDALVLPGGESTTMRRLIDRYGLRQPILAMAGRGAP
                     MLGTCAGMILMAGRTNDDEEPALGMLDVAVQRNAYGRQLDSFEMDVDMPSLGTQPLHG
                     VFIRAPVVADVGPGVEVLARDAEGRPIAVRQGKVLATAFHPELTGDRRLHRLLLELVG
                     E"
BASE COUNT          481 a         1141 c         1202 g          464 t
ORIGIN      
        1 catgcgcagg agctcgtcgc gcacccgctc gccggagagc gaggccgccg cctccgcgtt
       61 gcgctggatg gcggctgccg tggccggatc cagcgccagc cggtaccgga ctgcgaagcg
      121 caccgcgcga acaaggcgca gcgcatcctc ggcaacccgc tggtccgggt cacccacagc
      181 ccgcagcacg cgtcgctcaa ggtcggcggc accctggtgg ggatccacca gcattccctc
      241 gccggcatcc aggtcggtcg ggcgccaggc catggcgttg acggtgaagt cgcggcgggc
      301 caggtcctcc tccaacgacg ttccccagcg caccgactcg ggccgccgcc ggtcgcggta
      361 ggtctcctcc acgcggaagg tggtcacctc caccctcggc tcgtccggct ggcccgatac
      421 ggtgaccgtg ccgaacgggt tctgccagct cgaaccgggg aaggcggcgg ccaccgattc
      481 aggtggcagc gaggtggcga cgtcccagtc cccgggcggc tccccggcga ccaggtcgcg
      541 gacgcagccg cccaccagga cggcgtcgcc gccgcgctcg gccaggcccg agagcacgtg
      601 gaccaccacc cggggaagtt cgatgcgcat ccggtcgccg tcgcccatcg gtccggcgca
      661 ggccgtcagg acccttcggc gggggaaggc tccaggaaca ggtagctgcg ccaggcgtcg
      721 ttgcgctcgt ccgacaggta ggagccgaac aggtagcgca cgtccgcgcg cgtggcgcgg
      781 ggctcgcgga gcaggtgcat ccgcgcctcg gccagcgtct tgctcccctt gcggtggttg
      841 cagctccggc aggcggccac caggttttcc cactcgtgac ggccgccgcg gtgcttgggc
      901 accacgtggt cgatggtcag gtcgcggctg actgtgccgc agtactggca ggtgtgccgg
      961 tcgcgggcga agatctcgcg ccggctcagc ttgaccctgg ggcgcgggcg cttgatgtgg
     1021 tactggagcc gaatgaccga tggcgccggg atctcgatcg tcggcgtatg gatcacctgg
     1081 tggttccgct ccaggagctc ggccttgctg gcgccgagca ggcggaaggc gcgcctgatg
     1141 tcgcagacgt tgaggggttc gtagttctgg ttgagcacga gtaccggtgc gttgatcatg
     1201 agcggagcct tcctgccgcc gatcatacca accctcgcgc tcgcgctccc tctcacagcc
     1261 tggctgcctt gcccctgtac cgggcaggag ttcacccgag ggccgccgca cgagccgttc
     1321 tgccggtgta gcgggcagcc gtcagcgtga gcggcgaaga acttgtcggt tcctgcgtgg
     1381 ggctgcccgc cgtaccagcg gaatgacacg cagaccgagc ctcccgtgca gaactgcccg
     1441 atatgacggg catcagcacg ctggcgtggt cgaccagcgg caagcgccga cttggcaatc
     1501 atcccgcgga taattggcac ccggaggcgg cgcgtgggcg cgttcccggg gccgcagtag
     1561 aattcgaggc acaggcggcg cgcggcgccg ccatcggtcc atgcaggaag cacgaatgac
     1621 gaccatcgaa cgatcgacct gggccaccaa ggtgggactg gcacgcatgc tctccggcgg
     1681 cgtgatcatg gacgtggtga ccgccgacca cgcccggatc gccgaggagg cgggagccgt
     1741 ggcggtgatg gccctggaac gcgtcccgag cgacatccgc aagttcggcg gcgtggcgcg
     1801 catgagcgac cccaagctga tcgaggagat caaggccgcg gtcagcatcc cggtcatggc
     1861 caagtgccgc atcgggcata tcgccgaggc ccagatcctc cagcagctgg acgtggactt
     1921 catcgatgag tcggaggtcc tgaccccggc cgacgaggag caccacatcg acaagcacca
     1981 gttcaaggtg ccattcgtgt gcggctgccg ggacctgggc gaggcgctgc ggcgcatcgg
     2041 tgagggggcg gccatgatcc gcaccaaggg cgaggccggc accggcaaca tcgtggaggc
     2101 cgtccgccac atgcgcgccg tgatgaaggg catccgcgag ctgtccggca tgcgcccgga
     2161 cgagcggttc gccgtggcca aggagctgcg cgcgccgctg gacgtggtgc agcagaccgc
     2221 cgagctgggc gagctgccgg tgccgaactt ctcggccggt ggcgtggcca ccccggccga
     2281 tgccagcctg atgcgccagc tgggcgcaca ggcggtgttc gtcggatcgg gcatcttcaa
     2341 gagcgagaat ccggagcgcg tggccaaggc catcgtgctg gccaccaccc actacgacaa
     2401 cccggagatc ctggccgagg tcagcgccgg tttgggcgag ccgatgcacg ggatcgagct
     2461 cggcgagatc ccgctggagg agcggctggc cgtccgcggc tggtagggct ggcataccaa
     2521 tggcgcaacc ggcgccgtcc gcgcctctac ccgagtccgg ggccgagctc gagccgagcc
     2581 cggtcggcgg tccgtcacgc cgccgcccgc gggtgggcgt attggccctg cagggcgcgg
     2641 tccgcgagca catggacgcc ctgcgcgaga tcggtgcgga gccggtggcc gtccgcctgc
     2701 cgcacgacct cgacgggctg gacgccctgg tgctccccgg cggcgagtcg accaccatgc
     2761 ggcggctcat cgaccgctac ggcctccgtc aaccaatcct ggccatggcc ggtcggggcg
     2821 cgccgatgct gggcacctgc gccggcatga tcctgatggc cggccgcacc aacgatgacg
     2881 aggagccggc gctggggatg ctggacgtgg cggtgcagcg caatgcctac ggccgccagc
     2941 tcgactcgtt cgagatggac gtcgacatgc cgtcgctcgg cacccagccg ctgcacgggg
     3001 tcttcatccg cgcccccgtg gtggccgacg tggggcccgg cgtcgaggtg ctggcccgcg
     3061 acgcggaggg ccggcccatc gccgtgcgcc agggaaaggt gctggccacc gcgttccatc
     3121 ccgagctgac cggggaccga cgcctgcatc ggctcctgct ggagctggtg ggcgagtgac
     3181 ggtcggcggc gccatccggc ccgcgcggct cagtgaccgg gccgcgctgg gggagctgtc
     3241 ccggcgcgtc cacctcagcc cggacagcta ccggcgctca ctgggggt
//
LOCUS       DATACA010000428         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2066321, whole genome shotgun sequence.
ACCESSION   DATACA010000428 DATACA010000000
VERSION     DATACA010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066321"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VHK28_08725"
     CDS             complement(<1..484)
                     /locus_tag="VHK28_08725"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142336.1"
                     /translation="MTDAGGSSAPPHAGFDPELRIDWVEADELGDGRPGRLGLTFLPG
                     KRGASTRYPGHVYRRDTATDLAEMRRRGIGRLILLVDDDELAQWGDSRIVEIGRALGV
                     EVRRFPMADGSPPASAAEMDAILAATRQARATTDVAVACMGGVGRSGLVAACALIRAG
                     L"
     gene            complement(484..1014)
                     /locus_tag="VHK28_08730"
     CDS             complement(484..1014)
                     /locus_tag="VHK28_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016467874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX2142337.1"
                     /translation="MARRLLIFNAPDRFVAGAIGEPGDRTFYLQARQGNAVASVALEK
                     LQVAALAARLDDLLTAVADEAPAPTEVTADEGPLEEPVIDLFRVGAMALAWDSGGGRV
                     VIEAQPLSEDGDYVEVPDDVEEGPDVLRVAISPAHAHEFVRRASALVAAGRPACPFCG
                     ESLEPGGHFCPKVSLN"
     gene            1140..1619
                     /locus_tag="VHK28_08735"
     CDS             1140..1619
                     /locus_tag="VHK28_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142338.1"
                     /translation="MGWGAAALIMVAIAFIVGRPGGEQGLLSATPSATPTPPLPIVFG
                     TGLDPATNQATGATDRFRNGDPFAYSVTLQAAPAVEEILVEVVRLDGQERTVVQEPSV
                     QRILPEPRTFAFQVLAADLLAAWGAGEFEMRIWLRSGATPLATGRFTLLASPEESAG"
     gene            complement(1804..3102)
                     /locus_tag="VHK28_08740"
     CDS             complement(1804..3102)
                     /locus_tag="VHK28_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2142339.1"
                     /translation="MPRTKAIARATEHDAALARQIGARIRESRRRAGLTQQQLAGERY
                     TKAYVSALETGIARPSMVALSYLSERLGLPPSHFLDEQSPIWTRLEVDMQLASGQWQA
                     AAEGYASLLESSLDDPSRAEVLRGQAEALVRLDRGREAVTAAAEAARLFGALGKVSDE
                     ALARYWLAYGLYLSDAEADARSMLTGLLERVRGGLKVEPEFEMRLLMALAAVESRMGN
                     YQHSLTHLEAARGMADDLDDRRRAMFLYNLAISYRETGDVEAAIRAGNHGLALLRAAG
                     SAFESAQIENDLAMAYLATGDVPRARQLAGQARREFEQAGDDRYLSAVLETEARVALA
                     AGERDEAVTLARRAAGLARDAGNRAVQLTAMLTEARALREAGDLATAESRYAEAAGLA
                     REGQVASRLRDVLREWADLRAESGDHRGAYELTSEALTVG"
     gene            complement(3380..>3649)
                     /locus_tag="VHK28_08745"
     CDS             complement(3380..>3649)
                     /locus_tag="VHK28_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2142340.1"
                     /translation="VSWEWTKRDRKGRARLDYTQNAVNKTLVAPYSVRPAPGAPVSVP
                     IKWEELEDPELRSDRWTIATTFDRLSEVGDLFAPALELEQELPPL"
BASE COUNT          532 a         1361 c         1215 g          541 t
ORIGIN      
        1 cgagccccgc acggatcagg gcgcaggcag ccaccagtcc gctgcgtccc accccgccca
       61 tgcaggcaac cgcgacatca gttgtagcgc gagcctgccg cgtggcggcc aggatggcat
      121 ccatctcggc cgcggaggcc ggtggcgacc cgtccgccat cggaaagcgg cgaacctcca
      181 cccccagcgc ccgcccgatc tccacgatgc gcgagtcacc ccactgcgcc agctcgtcat
      241 cgtccacgag caggatcagc cgtccgatgc cgcgcctgcg catctcggcc aggtccgtgg
      301 cggtgtcgcg gcggtagacg tgccccgggt agcgggtcga ggcgccgcgc ttgcccggca
      361 ggaaggtcag gcccagccgt cccggccggc cgtcgcccag ctcgtccgcc tccacccagt
      421 cgatgcgcag ctccgggtcg aatcccgcgt gcggcggagc ggaagagccg cccgcatcgg
      481 tcatcagttc aggctgacct tggggcagaa gtggccgcct ggctcaagcg actctccgca
      541 gaaggggcag gccggacggc cggcggcaac cagcgcactg gcacgacgga cgaactcgtg
      601 ggcatgcgct ggggagatgg ccacccgcag cacgtccggt ccttcctcca catcgtccgg
      661 gacctccacg tagtcgccat cctcgctgag cggctgggcc tcgatgacca ccctgccacc
      721 gcccgaatcc caggccagcg ccatggcacc cacccgaaac aggtcgatca ccggctcctc
      781 cagcggcccc tcatcggctg tcacctcggt tggtgccggc gcctcgtcgg ccacggcggt
      841 cagcaggtca tccaggcgtg cggcgagggc cgcgacctgc agcttttcga gcgccacgct
      901 ggccacggcg ttgccctgcc gggcctgcag gtagaaggtc cggtcgcccg gctcaccgat
      961 ggcgccggcc acgaatcggt caggggcatt gaagatcagg agtcgacgcg ccatatcggc
     1021 aatcgtagcc cggcacggcc ggtagagtgt ggcgcgacat ggtgcgctcc cgctcccatg
     1081 cgcgtcgact tcggccagag gcacggccgc gccgccggac gccccgcggc gcgtcgctga
     1141 tgggatgggg agcggcggcg ctgatcatgg tcgcgatcgc cttcattgtc ggccgtcccg
     1201 gcggcgagca gggtctgctc tcggcaaccc cctccgccac gccgaccccg cccctgccga
     1261 tcgtcttcgg caccggcctg gaccccgcga ccaaccaggc cacgggcgcc actgaccgat
     1321 tccgcaacgg tgacccgttc gcgtactcgg tgacgctcca ggcggcacca gccgtggagg
     1381 agatcctggt ggaggtcgtg cgcttggatg gtcaggaacg gacggtggtg caggagccat
     1441 cggtccagcg catcctgccc gagcccagga cgttcgcctt ccaggtacta gctgccgacc
     1501 tgctggcggc gtggggggcg ggcgaattcg agatgcggat ctggctgcgg tccggtgcga
     1561 ctccgcttgc cacggggcgc ttcacgctgc tggcgtcgcc ggaggaaagc gccgggtaga
     1621 ccacgggatc ggagatgtgc ccgctctccg atcccgtctc ccccggcttc gagctggtcc
     1681 gccgctgtag cagcgggtgc ccacaccaac tcgttcgggt cagcctatcc gcaccacacc
     1741 ggacctgtca atactccttt gaccaagtgc tcagcctatt tgaccgatgc cggcgaggac
     1801 gcctcacccg acggtcagcg cctcgctggt cagctcgtag gcaccgcgat ggtcgccgct
     1861 ctcggcccgc aggtctgccc actcgcggag cacgtcccgc agccgcgacg cgacctgtcc
     1921 ctcccgggcc agcccggccg cttccgcgta gcggctctcg gccgtggcca ggtcccccgc
     1981 ctcgcgcagc gcacgcgcct cggtgagcat ggcggtcagc tgcacggcgc ggttgcccgc
     2041 gtcacgggcc agtccggccg cgcggcgcgc cagcgtcacc gcttcgtcac gctcgccggc
     2101 ggcgagggcc acgcgggcct ccgtctccag cacggctgac aggtatcggt cgtctcccgc
     2161 ctgctcgaac tcgcgccgcg cctgcccggc cagctgacgc gcacgcggaa catccccggt
     2221 tgccaggtag gccatggcca ggtcgttctc gatctgggcc gattcgaacg ccgatccggc
     2281 agcccgcagc agggcgaggc cgtggttgcc ggcacggatc gcagcctcca cgtctccggt
     2341 ctcccggtag ctgatggcaa ggttgtagag gaacatggcg cgacgccggt catcgaggtc
     2401 gtcggccatg ccgcgcgctg cctccaggtg cgtcagcgag tgctggtagt tccccatccg
     2461 tgactcgacc gcggccaggg ccatcagcag ccgcatctcg aactccggct ccaccttcag
     2521 cccaccgcgc acccgctcca gcaggccggt gagcatgctg cgtgcgtccg cctccgcgtc
     2581 cgagaggtag aggccgtagg ccaaccagta gcgcgccagc gcctcgtccg acaccttgcc
     2641 cagcgcgccg aagaggcgcg ccgcctcggc ggcggcggtg accgcctcgc gaccacggtc
     2701 caggcggacc agcgcctccg cctggccacg cagcacctcc gcccgactgg ggtcgtcgag
     2761 agatgattcc aggaggctcg cgtagccctc cgcggccgcc tgccactggc ccgaggcgag
     2821 ctgcatgtcg acctccaggc gcgtccagat gggcgattgc tcatccagga aatggctggg
     2881 cggaaggcca aggcgctcgg agaggtagct cagcgcgacc atggatgggc gcgcgatccc
     2941 ggtctccagg gcactgacgt aggccttggt gtagcgctcc cccgccagct gctgctgggt
     3001 caggccggcg cgccgccgcg actcgcggat ccgcgccccg atctggcggg caagcgccgc
     3061 atcgtgctcg gtggcgcggg cgatggcttt ggtgcgcggc atggacgcag tgtacacgcg
     3121 ctggcaagcg ttacttgacg cctcccagca ccaaaagtac cccggcccgt cgaaggccgg
     3181 cgtaccgacg ggtcattgcc cggcggacct ccgtcgcgga acgtgatctg gcaatcgcaa
     3241 cctgtacaag gagcacgcca gatgcgcagc gcaagctgtg gcggcggctg gcctaactaa
     3301 gccacctcgc cctttgattg ggggacgtgt cgcggaagcc cactcgccaa gtgatgggag
     3361 gccgcgacgc gttttttcgt tacagcggcg gcagctcctg ctccagctcc agcgcgggag
     3421 cgaacaggtc tcccacctcc gacaaacgat cgaaggtcgt cgcgatcgtc catcggtccg
     3481 accgcagctc tggatcctcc agctcctccc acttgatcgg gaccgatacc ggcgcgcccg
     3541 gcgctggccg caccgaatac ggcgcgacaa gggtcttgtt gaccgcgttc tgcgtgtagt
     3601 ccaggcgcgc ccgacccttc cgatctcgct tggtccactc ccagctcac
//
LOCUS       DATACA010000429         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1905668, whole genome shotgun sequence.
ACCESSION   DATACA010000429 DATACA010000000
VERSION     DATACA010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905668"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VHK28_08750"
     CDS             <1..358
                     /locus_tag="VHK28_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142341.1"
                     /translation="PRSELDATVAALAESLRRKLPETIRYTKAQLNWWRDLVWSQTIT
                     HARDWLAVHSGSDEVREAVSAFHEKRAPEYEALRTAATDGGRACPSCGERGLSARYEY
                     CPMCGAGLTALQEGVP"
     gene            462..1250
                     /locus_tag="VHK28_08755"
     CDS             462..1250
                     /locus_tag="VHK28_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2142342.1"
                     /translation="MNASTQPLILVERQDDQRTALVRLNRPKQLNALNAQVMDELCAT
                     LEDLDRDESVRAIVVTGNERAFAAGADIGEMADATPVDMLKGNRIGQWDRIRRIGKPV
                     IAAVNGWALGGGCELAMTLDLIIAGEGAKFGQPEINIGVIPGAGGTQRLTRAIGKSRA
                     MEMILTGEPMDAREAERRGLVARVVPDELVVEDALTLAAKIATKSPIALRLAKEALNA
                     AYEMSLTDALAHERRLFYLLFASEDQKEGMAAFLEKRTPDFNGR"
     gene            1247..2218
                     /locus_tag="VHK28_08760"
     CDS             1247..2218
                     /locus_tag="VHK28_08760"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142343.1"
                     /translation="MSLGNPDHPVGGIAVRPPAGVELRPLGRADLAHALALAREQREL
                     PWVTDVEAVRDRYDRLINTADTAAFLAMAEGVPVGFSALQFRRRLNFATFEGWLSDLY
                     VRPAFRRRGIGRALVEAVVAEWRLRGSHRLQAQVLEEGTAGATLERAGFEEWMLDFQL
                     APVIAPPQQVPEGVSLRPVRPDDGEAVTRLIAEFGPHRSPVSDRMEAVLRTFAEHLRA
                     VETGAATTTIAELEGKAVGVCVMEWREPFWTDAVQAWIHDVVVTEPLRGRGIGRALMA
                     DALGRAVQRGAAAVLLESAMQRESAHRLYRSLGFEEAGRTWVLRRDP"
     gene            2451..>2786
                     /locus_tag="VHK28_08765"
     CDS             2451..>2786
                     /locus_tag="VHK28_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142344.1"
                     /translation="MGEEPSGLPAVAVLMPKTHGDLEDLRPDVIGELGGMIQRLARAF
                     GRLDSVGRVHVNRWGDGSEHFHVWFLARPRGIWQLRGAMLAVWDDILPRVPVEEWLAN
                     RRTIAAALAE"
BASE COUNT          423 a          939 c         1018 g          406 t
ORIGIN      
        1 gccgcgttcc gagctggatg ccaccgtggc tgcgctggcg gagagcctgc gccgaaagct
       61 ccccgagacg atccgctaca ccaaggcgca gctcaactgg tggcgcgacc tcgtctggtc
      121 gcagaccatc acccatgcgc gtgactggct ggcggtgcac tccggctccg acgaggtgcg
      181 cgaggcggtg agcgccttcc acgagaagcg agcgcccgag tacgaggctc tgcgcacggc
      241 ggcaaccgac ggcggccggg cgtgcccttc ctgcggagag cggggcctgt ccgcgcgcta
      301 cgagtactgc ccgatgtgcg gcgcaggcct cactgccctc caggagggag tcccttgatg
      361 acttacgcgc gcgccgggcg gcacatggga ccccctgttt gctgcacgga tgctgtgctg
      421 cacagccgca cgatcaggcc tcacagcgcc agcgcggccc gatgaacgcc tccacccaac
      481 cgctgatcct ggtcgaacgc caggacgacc agcgcaccgc cctggtgcgc ctcaaccggc
      541 cgaagcagct caacgcgctg aacgcccagg tgatggacga gctgtgcgcc acgctcgagg
      601 acctggaccg cgacgagtct gtccgagcga tcgtggtcac cgggaacgag cgtgcgttcg
      661 ccgcgggcgc agatatcggt gagatggcgg acgcaacgcc cgtcgacatg ctcaagggca
      721 accgcatcgg ccagtgggat cgcatccggc gcatcggcaa gccggtgatc gccgcggtca
      781 acggctgggc cctgggtggt gggtgcgagc tggcaatgac cctggacctg atcatcgccg
      841 gggagggcgc gaagttcggg cagccggaga tcaatatcgg cgtcatcccg ggtgcgggcg
      901 ggacgcagcg gctgacccgc gccatcggca agtcccgcgc catggagatg atcctgaccg
      961 gcgagccgat ggacgcccgc gaggctgagc gccgcgggct ggtggcgcgc gtggtcccgg
     1021 acgagctggt ggtggaggac gcgctgacgc tggctgcgaa gatcgccacc aagtcgccga
     1081 tcgcgctgcg cctggccaag gaggctctga acgccgcgta tgagatgagc ctgaccgatg
     1141 cgctggccca cgagcgtcgc ctcttctacc tcctcttcgc cagcgaggac cagaaggagg
     1201 gcatggcggc cttcctggag aagcggacgc ccgacttcaa cggccgatga gcctgggcaa
     1261 ccccgaccat cctgtcggcg gcatcgccgt ccgtccgccg gccggcgtcg agctgcggcc
     1321 gctcgggcgc gccgacctgg cgcacgcgct ggcactggcg cgggagcaac gcgagctgcc
     1381 atgggtcacc gatgtcgaag ccgtccggga ccgatacgac cgcctgatca acaccgccga
     1441 caccgccgcc ttcctggcaa tggcggaagg tgtgccggtc ggattctctg ccctccagtt
     1501 ccgaaggcgc ctgaacttcg ccaccttcga gggctggctg tcggacctgt acgtgcgacc
     1561 ggccttccgt cgacgcggca tcgggcgcgc gctggtggag gcggtcgtcg cggagtggcg
     1621 gctgcgcggc agccatcggc tccaagcgca ggttctcgag gaggggactg caggtgccac
     1681 cctggagcgc gcgggcttcg aggagtggat gctcgacttc cagctggccc cggtgatcgc
     1741 gccgccacag caggtaccgg agggagtcag cctgcggcct gtccgccctg acgacggcga
     1801 ggccgtgacc cgactcatcg ccgagttcgg gccgcaccgc tcgccagtgt cggatcggat
     1861 ggaggccgtg ctgcgcacct tcgccgagca cctccgcgcc gtcgagaccg gcgctgccac
     1921 gaccaccatc gccgagctcg agggtaaggc ggtgggcgtg tgcgtcatgg aatggcgcga
     1981 gccgttctgg accgatgccg tgcaggcctg gatccacgac gtggtggtca ccgagccgtt
     2041 gcgcggccgc gggatcggac gcgccctcat ggcggatgcg ctgggccgcg cggttcagcg
     2101 cggagccgca gccgtgctgc tcgagtcggc catgcagcga gagtcggcgc atcggctgta
     2161 tcggtccctc ggcttcgagg aggctgggcg cacctgggtg ctgcgccggg acccgtgagc
     2221 gagcgagcca gcgactacgt tcggcgcctc ccgatcgggg agcgcctgcc cattggcgag
     2281 gtgatcaagg acgacctctt tccattcgag gggacatcct ggtaagacca ctgaccgagc
     2341 cgatcctccc cgagccgcta cgcaacggcg aggtgggagg tggcgagtgt cacgcctgcc
     2401 atggaaccgg ggaaggcctg atctgggagg acgagcgctg gtgggccttc atgggcgagg
     2461 agccctcggg gctgcccgcg gtagccgtgc tcatgcccaa gacgcacgga gacctggagg
     2521 acctgcggcc ggatgtgatc ggcgagctgg gcgggatgat tcagcgcctt gcccgcgcgt
     2581 tcgggcgcct ggactcggtg ggacgcgtcc acgtcaaccg ttggggtgac ggcagcgagc
     2641 acttccacgt ctggttcctg gcccgaccgc gcgggatctg gcagctccgt ggcgcgatgc
     2701 tggccgtatg ggatgacatc ctgccccgcg tgcccgtcga ggagtggctg gccaaccggc
     2761 ggaccatcgc cgcggcacta gcggag
//
LOCUS       DATACA010000430         1853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1423728, whole genome shotgun sequence.
ACCESSION   DATACA010000430 DATACA010000000
VERSION     DATACA010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423728"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VHK28_08770"
     CDS             <1..316
                     /locus_tag="VHK28_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase DnaB"
                     /protein_id="HEX2142345.1"
                     /translation="ADREGLKAKRLRNHHLRGSSAINYESDIILIMNDKYHIVAKVNI
                     EFNPYQAQRYRDWVVVTVEKNRGGQDNVDLEYEKLFEYSCFDPTGRQTAEKLIEERLY
                     TD"
     gene            351..803
                     /locus_tag="VHK28_08775"
     CDS             351..803
                     /locus_tag="VHK28_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX2142346.1"
                     /translation="MLDPYRVLQVQPTAEQEVVNAAFRALALKYHPDRDSSPAAARRM
                     ALINEAYQLVRSAAKREAFNRAQLRSAWSAMPGGAAASARQASVPPPPRSASAGTRLE
                     FGRYSGWTLRDLARHDPDYLRWLSRHASGLRYRTEIYSILGRMGATAA"
     gene            844..1575
                     /locus_tag="VHK28_08780"
     CDS             844..1575
                     /locus_tag="VHK28_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2142347.1"
                     /translation="MNQLRAVFLDIGDTVMRPNPSWEHIYQLAFEEHGVEVDIDALRD
                     ALRAAYHHGGWGFEGGFEASEETSYRRTVEIDQAAIAELGIGPMPDSFFRRLSEMFLV
                     VSNWHVFPDALPAIQALKRRGLVVGAVSNWVWQLPELLHSLELVSHFDFITASARVGC
                     EKPHPEIFRHALREAGVRPDEALHVGDHLDADVAGAQGVGINAALIDRRERFGPEDMP
                     PGVPLIRTLTELVPMVDASVERASA"
     gene            1578..>1853
                     /locus_tag="VHK28_08785"
     CDS             1578..>1853
                     /locus_tag="VHK28_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX2142348.1"
                     /translation="MAEPAATERWRLFVAVPTTPELRSVLAETVSALRARPGADDDWR
                     WTDADAWHLTLAFLGRTDASRVPELRTAQAAGICHEGFKSRHSRGQGG"
BASE COUNT          297 a          639 c          631 g          286 t
ORIGIN      
        1 tgccgaccgc gagggcctga aggccaagcg actgcgcaac caccacctgc gcggctcctc
       61 ggccatcaac tacgagagcg acatcatcct gatcatgaac gacaagtacc acatcgtcgc
      121 caaggtcaac atcgagttca acccgtacca ggcccagcgc tatcgcgatt gggtggtggt
      181 cacggtggag aagaaccgtg gcgggcagga caacgtcgac ctcgagtacg agaagctgtt
      241 cgagtacagc tgcttcgacc cgacggggcg gcagaccgca gagaagctga tcgaggagcg
      301 gctctacacc gattgacgcg aggggaatcg gtgtctcgcc ggcaaggacc atgctggatc
      361 cctatcgcgt gctgcaggtg cagcccaccg ccgagcagga ggtcgtgaac gcggccttcc
      421 gggcccttgc gctcaagtac cacccggacc gcgactcctc gcctgcggcc gcccggcgca
      481 tggccctcat caacgaggcc taccagctcg tgcgcagcgc cgccaagcgc gaggcattca
      541 accgtgccca gctcaggagt gcctggagcg ccatgcccgg cggggctgcg gcgtccgcgc
      601 gccaggcatc ggtccctccg ccgccccgct ctgccagcgc cggcacacgc ctggagttcg
      661 gccgttattc cggctggacg ctgcgcgacc tggctcggca tgaccccgac tacctgcgct
      721 ggctctcgcg ccacgcatcg ggcctgcgct accgcaccga gatctactcg atcctcggcc
      781 gcatgggagc cacggcagcc tgatcgcggc cggtgcatcg gcagtttcgg tagtcttgga
      841 ccgatgaatc agctgcgcgc cgtcttcctg gacatcggcg acaccgtgat gcgccccaac
      901 ccgtcctggg agcacatcta ccagctcgcc ttcgaggagc acggggtcga ggtcgatatc
      961 gacgccctgc gcgacgcgct gcgtgctgct taccaccatg gggggtgggg cttcgagggc
     1021 gggttcgagg ccagcgagga gacgagctac cggcgaacgg tcgagattga ccaggcggcg
     1081 atcgccgagc tgggcatcgg tcccatgccg gactcattct tccggcggtt gtcggagatg
     1141 ttccttgtgg tcagcaactg gcacgtcttc ccggatgcgc tgccggccat ccaggcgctc
     1201 aagcgccgcg gcctggtggt gggtgcggtg agcaactggg tctggcagct gccggagctg
     1261 ctccactcgc tggagctggt gagccatttc gacttcatca ccgccagtgc ccgcgtcggc
     1321 tgcgagaagc cgcatccgga gatcttccgt cacgcgctgc gggaggccgg cgtgcggccg
     1381 gacgaggcgc tccacgtcgg tgaccacctt gacgcggacg tggccggtgc ccagggagtg
     1441 ggcatcaatg ccgccctgat cgaccgtaga gagcgcttcg gtccggagga catgccgccc
     1501 ggcgtcccgc tcatccggac cctgaccgag ctggtgccca tggtggacgc cagcgtcgag
     1561 cgggcaagcg cctgaaggtg gccgagccgg cggcgaccga acgctggcgg ctgttcgtgg
     1621 ccgtgccgac gaccccggag ctgcgttccg tgctggccga gacggtgagc gccctgcgtg
     1681 cccgtccggg tgcggatgac gactggcgct ggaccgatgc cgacgcctgg cacctgaccc
     1741 tggccttcct gggccggacc gatgcctcca gagtgcccga gctgcggacc gcccaggccg
     1801 ccggcatctg ccacgaaggg ttcaaatccc gccacagcag aggccagggc ggt
//
LOCUS       DATACA010000431         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1744062, whole genome shotgun sequence.
ACCESSION   DATACA010000431 DATACA010000000
VERSION     DATACA010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744062"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VHK28_08790"
     CDS             complement(<1..604)
                     /locus_tag="VHK28_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142349.1"
                     /translation="MSWRRTLAITRRLLSQFRHDHRTVALLLVAPIVLLGLFALLFRS
                     DGQPPTIGVIESDSGPLGDAVVEQLRGSELVTLRGADEGSQDMEAALDEGEMAGYVVF
                     PETFSQRAMSEGVIAPVLRLRGTDPAASGAVTRAIGTASLAALDDLLPPGSNVGPPRL
                     ELEPSFLFGGEELDTLDQLGGPFIGLVVFFLVYVVTSVSFL"
     gene            complement(601..1320)
                     /locus_tag="VHK28_08795"
     CDS             complement(601..1320)
                     /locus_tag="VHK28_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142350.1"
                     /translation="MSTPGGAMAVDARGVTKRFGSLTALDGVDLRVPTGQVFGLLGPN
                     GSGKTTLIRSLIGLVRPEAGELDVLGRRMPDVGILSEIGYMTQAPALYADLTAEENLR
                     FFATIHGGGDVEAALRFVHLEERRRSVVATLSGGMRTRLSLACTIVHRPRLLLLDEPT
                     VGVDPRLRAELWDGLRAMAADGATIIVSSHVMDEAERCDRLGLILAGRILADGSADEL
                     RDRAGSDRLEDAFLALSGGPS"
     gene            complement(1317..>1811)
                     /locus_tag="VHK28_08800"
     CDS             complement(1317..>1811)
                     /locus_tag="VHK28_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142351.1"
                     /translation="TAGVSRGTLYRLFPGKAALLRSMVESFAPFEAIHRSLATHGEQP
                     ADVVLPEIARTVVGIAEGRMGLMRAIFHEATGGSPTSVAGVRPVLQSALGALSVYISG
                     EMAAGRVRRMEPLLALQAFIGPIYFHLMTRPVAEEVMGLRTPADQAVQQVVQAVLHGL
                     LPDR"
BASE COUNT          291 a          651 c          645 g          224 t
ORIGIN      
        1 gaaggaagga gaccgatgtc accacgtaca ccaggaagaa gaccaccagg ccgatgaacg
       61 ggccgcccag ctggtcgagc gtgtcgagct cttcgccacc gaagaggaag ctcggctcga
      121 gctccaggcg gggcgggccc acgttggagc ccggcggtag caggtcgtcc agggcggcga
      181 ggctggccgt gccgatcgcc cgggtcaccg cgccgctggc cgcggggtcc gtgccgcgca
      241 gccgcagcac cggggcgatc acgccctcgg acatcgcgcg ctggctgaac gtctcgggga
      301 agaccacgta accggccatc tcgccttcgt ccagggcagc ttccatgtcc tgtgagccct
      361 cgtcggcccc gcgcagcgtg accagctcgg atccgcggag ctgctcgacc acggcgtcac
      421 ccagcggacc gctgtccgac tcgatcaccc cgatcgtcgg cggctggcca tccgagcgga
      481 acagcagtgc gaagaggcca agcaggacga tgggcgccac cagcagcagg gcgaccgtgc
      541 gatggtcgtg tcggaactgg ctgaggagcc gccgggtgat ggccagcgtc cgccgccagc
      601 tcacgacggg cctcccgaga gggcaaggaa cgcatcctcc agccggtcgc tgccggctcg
      661 atcccgcagc tcatcggcgc tgccgtcggc gaggatgcgg cccgccagga tgagccccag
      721 ccggtcgcag cgctcggcct cgtccatgac gtggctggag acgatgatgg tcgctccgtc
      781 cgccgccatc gcccgcagcc cgtcccacag ctcggcccgc aggcgggggt cgacgcccac
      841 cgtcggctcg tcgaggagca gcaggcgcgg gcggtggacg atggtgcagg ccagcgacag
      901 ccgggtgcgc atgccgccgg acagggtggc gaccaccgag cggcgccgct cctcgagatg
      961 cacgaaccgc agcgccgcct ccacgtcgcc gccgccgtgg atggtggcga agaagcgcag
     1021 gttctcctcc gccgtcaggt ccgcgtagag cgcgggcgcc tgggtcatgt acccgatctc
     1081 ggacaggatc cccacgtccg gcatccgccg ccccaggacg tccagctcac cggcctccgg
     1141 tcgtacgagg ccgatgagcg agcgaatcag cgtcgtcttg cccgacccgt tcggccccag
     1201 caggccgaag acctgaccgg tcggaacgcg aaggtccacg ccgtccagcg cggtgagcga
     1261 gccgaaacgc ttggtgaccc cgcgagcatc gacggccatg gcgccgcccg gcgtgctcat
     1321 cggtcgggaa ggaggccgtg caggacggcc tgtacgacct gttgcacggc ctggtcggcg
     1381 ggcgtccgga gtcccatcac ctcctcggcc accggtcggg tcatcaggtg aaagtagatg
     1441 ggaccgatga aggcctgcag cgcaagcagc ggctccatgc ggcgcacgcg gccggccgcc
     1501 atctccccgc tgatgtacac ggacagcgcc ccgagcgctg actggagcac cggccgcaca
     1561 ccagccaccg aggtggggga gccgccggtc gcctcgtgga agatggcccg catcaggccc
     1621 atcctgcctt ccgcgatgcc gaccaccgtg cgggcgatct cgggcaggac cacgtcggcc
     1681 ggttgctcgc catgcgtggc caggctgcgg tggatggcct cgaagggcgc aaaggactcg
     1741 accatcgagc gcaacagggc cgccttgccg gggaacagcc ggtagagcgt gccgcgcgag
     1801 acgcccgcgg t
//
LOCUS       DATACA010000432         4677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_781218, whole genome shotgun sequence.
ACCESSION   DATACA010000432 DATACA010000000
VERSION     DATACA010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781218"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VHK28_08805"
     CDS             <1..810
                     /locus_tag="VHK28_08805"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX2142352.1"
                     /translation="VLHGPFGEDGTLQGFLELAGIPYTGAGVLASAVAMDKVVFKALM
                     EGHDVPVVRYAHLRRSAWQADPRSAVDDLQARIGPRSVVKPARLGSSIGMSVVHDPDE
                     LPAALDEAFGYDSKVIVEAYLPNARELECGVLGTEQPVVFEPGEVRSHKEWYDYEAKY
                     TAGMADVIPRAEVDPELAERVREMALRAYRAIDCAGMARVDFLISELGEVYVSEINTI
                     PGFTATSMFPKQAELAGFAFGELVARLIDLGLEQSQEGPMTPDPVGREDGS"
     gene            966..1676
                     /locus_tag="VHK28_08810"
     CDS             966..1676
                     /locus_tag="VHK28_08810"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX2142353.1"
                     /translation="MAAAVTGPWLRVEQVEHAGARFTPAAALDEMLAAYYGAALLAVD
                     SRGLAERIAGLPAVERVEVSSRLPNALAVTVHEKAPDFTWQTRAAVLVGAADGTIIAE
                     LPRDAEPTPELAALPAVHDDRRASRRITVGDALNAEELGMARRLMSLDATLLGSAAKT
                     LGVRLDDEHGFVLVCPDGGWEAALGVYGLDPRAEAGEAQRLLERQVAAIRTLFSVHPE
                     ASVSWLDARNPGKVYWAP"
     gene            1731..2054
                     /locus_tag="VHK28_08815"
     CDS             1731..2054
                     /locus_tag="VHK28_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HEX2142354.1"
                     /translation="MWLPLVGLALGIALGLVLNVNVNPDLARYSAVGILAGLDSVLGA
                     IRAELDAHYDNRVFLSGFVTNALVAVALTFVGDRLGIDLYLVALFAFGLRIFQNVALI
                     RRHFL"
     gene            2112..3350
                     /gene="ftsA"
                     /locus_tag="VHK28_08820"
     CDS             2112..3350
                     /gene="ftsA"
                     /locus_tag="VHK28_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX2142355.1"
                     /translation="MDRETVLVAIDAGTTKVTTLIGEVGRSGDVNIIGYGIAPSVGMK
                     KGMVANIDQTVNSIATSLEKAERLSGYKISSAFVGVGGSHITSTNSRGVVAVSGHKRE
                     VGREDVARATEAAKAIQTPSNREMLHVVPRGYIVDGQEGIKDPLGMSAVRLEVETHIV
                     FGAATSVQNLSKCIANANVQIDELVISSLAAAEATLTDTEKELGVLVADIGGGTTDIA
                     IFTDGAVYHSAVLPVGAINVTNDVAIGLRTSLNLAEEIKIKHGTADLRDVQPEELLNV
                     AVLGDGGGQTIQRRKLCEIIEARMSEMYSLIAEEVKRSGHSGMLPAGVVLTGGGARLG
                     GSAELARDVFQMPVRVGSPQGVGGLMDQLSNPAFSTPIGLLLWGAHHAGEEPIGYAAG
                     SPLSGVGSRVVDWVRGLFPG"
     gene            complement(3360..3818)
                     /locus_tag="VHK28_08825"
     CDS             complement(3360..3818)
                     /locus_tag="VHK28_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142356.1"
                     /translation="MSRGRVAVVDDNHETLDLLRDILTDHGYEVEVAPAVDAGLHEIR
                     DARPDIIIVDLLLSTDQRWLSGWDVVRLARSHAELHRLPIVVISADPGRLNTHRGEAA
                     RLTNVRLLPKPFGVDEIVGVVTELLDASAAANAARDGASQSEGVSQPATG"
     gene            4070..>4677
                     /locus_tag="VHK28_08830"
     CDS             4070..>4677
                     /locus_tag="VHK28_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX2142357.1"
                     /translation="MPLRSDAEHFALIKVIGVGGGGSNAVNRMIRAEMMGVEFIGVNT
                     DAQALLQSDAPHKIRIGDKMTRGLGAGADPQVGQRAAEEDSEKIYEALKDADMIFITA
                     GMGGGTGSGAAPVIAEIAKDLGALTVGVVTKPFSFEGMRRKLVAEQYTELLKEKVDTL
                     ITIPNDRLREVVDKKTSIVDAFRVVDDVLRQGVQGISDLITVP"
BASE COUNT          728 a         1547 c         1721 g          681 t
ORIGIN      
        1 gtgctccacg gtccgttcgg cgaggacggc acgctgcagg gcttcctgga gctggcgggg
       61 atcccctaca ccggcgccgg cgtgctggcc agtgcggtgg ccatggacaa ggtcgtgttc
      121 aaggccctga tggagggtca cgacgtgccc gtggttcgct acgcgcatct gcgtcggtcg
      181 gcctggcagg cggacccacg atcggcggtg gacgacctgc aggctcgcat cggtccgcgc
      241 tcggtggtca agccggcgcg cctgggaagc agcatcggga tgagcgtggt gcacgatccg
      301 gacgaactgc ccgcggcgct ggacgaggcc ttcggttacg acagcaaggt gatcgtggag
      361 gcgtacctgc ccaacgcgcg agagctggag tgcggagtcc tgggcaccga gcagccggtg
      421 gtcttcgaac cgggagaggt gcgcagccac aaggagtggt acgactacga ggcgaagtac
      481 accgccggca tggccgacgt gatcccgcgg gcggaggtgg accccgagct ggccgagcgg
      541 gtgcgggaga tggcgctgcg cgcataccgg gccatcgact gcgcaggcat ggcgcgcgtt
      601 gacttcctga tctcagagct gggcgaggtc tacgtgtccg agatcaacac catcccgggc
      661 ttcacggcga ccagcatgtt tcccaaacag gccgagctgg ccggcttcgc cttcggcgag
      721 ctggtggccc gcctgatcga cctggggctg gagcagtcgc aggaaggacc catgaccccc
      781 gacccggttg ggcgggagga tggatcgtga cgcgcgggaa gccacgtgcg gcggcccgac
      841 cccgtcggcg tggcgccgga caggctcgcc gacctcgtac cccgctgcgg tcgcggatcc
      901 ggcgcaggct gccatcccgg ggccgcgtcc tggccgtgct gctcgccgcg gcgctcggat
      961 cggccatggc ggcagccgtc actgggccgt ggctgcgcgt cgagcaggtg gagcacgcgg
     1021 gggcgcgctt cacgccggcg gccgcgctcg acgagatgct ggccgcctac tacggggcag
     1081 cgctgctggc ggtcgacagt cgcggcctgg cggagaggat cgcagggctg cccgcggtgg
     1141 agcgggtgga ggtcagctcg cggctgccca acgccctggc cgtcaccgtc cacgagaagg
     1201 cgccggactt cacctggcag acccgggcgg cagtgctggt gggggcggcc gacgggacca
     1261 tcattgccga gcttccgcga gatgccgagc ccacgcccga gctggccgcg ctgccggcgg
     1321 tccacgacga tcggcgtgcc agccgccgca tcaccgtcgg cgatgcgcta aacgccgagg
     1381 agctgggcat ggcccgccgg ctgatgagcc tggacgccac cctcctcggg tcggccgcca
     1441 agacgctggg ggtgcggttg gacgacgagc acggcttcgt gctggtctgc ccggacggcg
     1501 gctgggaggc ggcgctgggc gtctacgggc tcgacccgcg ggccgaggcg ggggaggcgc
     1561 agcggctgct ggagcgccag gtggccgcca tccgcaccct gttcagcgtc catccggagg
     1621 catcggtcag ctggttggat gcccgcaatc cggggaaggt atattgggcc ccgtagagac
     1681 tgccgcgctc ccgggcttga tccgcgcggc acgcagcgga agcgtcgcgg atgtggctcc
     1741 ccctggtcgg actggcgctg ggcatcgcgc tcggcctggt gctcaacgtc aacgtcaacc
     1801 cggatctggc gcgctacagc gcggtcggca tcctggccgg gctcgactcg gtgctgggcg
     1861 ccatccgcgc cgagctggac gcgcactacg acaaccgggt cttcctgtcc ggcttcgtga
     1921 ccaacgcgct ggtggctgtg gcgctgacct tcgtgggcga ccggctgggc atcgacctgt
     1981 acctggtggc gctgttcgcc ttcgggctgc gcatcttcca aaacgtggcg ctcatcaggc
     2041 gtcacttcct gtaggctcgg gcgcgcgccg cggcgccgcg accgcggcac ggcgcgacca
     2101 ccggaggtag cttggacagg gagacggtcc tcgtcgccat cgacgcgggg acgaccaagg
     2161 tgacgacgct gatcggcgag gtgggccggt ccggcgacgt caacatcatc ggctacggga
     2221 tcgcgccgtc ggtcgggatg aagaagggga tggtcgccaa tatcgaccag accgtgaact
     2281 ccatcgccac cagcctggag aaggcggagc gcctgtcggg ctacaagatc agctcggcct
     2341 tcgtgggcgt cggcggcagc catatcacca gcaccaacag ccgcggcgtg gtggccgtct
     2401 ccggccacaa gcgcgaggtc ggtcgcgagg acgtggcccg cgccaccgag gcggccaagg
     2461 ccatccagac cccgtccaac cgcgagatgc tgcatgtcgt gccgcgcggt tacatcgtgg
     2521 acggccagga gggcatcaag gacccgctgg gcatgagcgc ggtgcgtctg gaggtcgaga
     2581 cgcacatcgt gttcggtgcg gcgacatcgg tccagaacct gtccaagtgc atcgccaacg
     2641 ccaacgtgca gatcgacgag ctggtgatca gctcgctggc cgcggcggag gccaccctga
     2701 ccgataccga gaaggagctg ggcgtgctgg tcgccgacat cgggggcggc acaaccgaca
     2761 tcgccatctt caccgatggc gccgtctacc actccgcggt gctgccagtg ggcgccatca
     2821 acgtcaccaa cgacgtggcc atcggcctgc gcaccagcct gaacctggca gaggagatca
     2881 agatcaagca cggcaccgcc gacctgcgcg acgtccagcc ggaggagctg ctcaacgtgg
     2941 ccgtcctcgg cgacggcggc gggcagacca tccagcgtcg caagctgtgc gagatcatcg
     3001 aggcgcgcat gagcgagatg tactcgctga tcgccgagga ggtgaagcgc tccggccaca
     3061 gcggcatgct gccggccggc gtggtgctga ccggcggcgg ggcgcggctg ggcggctcgg
     3121 ccgagctggc gcgcgacgtg ttccagatgc cggtccgcgt cgggtccccg cagggcgtgg
     3181 gcggcctgat ggaccagctc tccaacccgg ccttcagcac gcccatcggc ctgctgctgt
     3241 ggggcgccca ccacgccggc gaggaaccga tcggctacgc ggccggctcc ccgctgagcg
     3301 gcgtggggtc gcgggtggtg gactgggtgc gggggttgtt ccccggctga gccgctcggt
     3361 cagcccgtag cgggttggct tacgccctcc gattgcgagg cgccatcccg ggccgcgttg
     3421 gcggccgcgc tggcgtccag cagctcggtg accacgccca cgatctcgtc cacgccgaag
     3481 ggctttggca gcagccgcac gtttgtcagg cgcgccgcct cgccgcggtg cgtgttcagg
     3541 cgcccgggat cggccgagat gaccacgatc ggcagccgat gcagctccgc gtgtgagcgt
     3601 gccaggcgga ccacgtccca gccggagagc cagcgctggt cggtcgagag cagcaggtcc
     3661 acgatgatga tgtccgggcg ggcgtcgcgg atctcgtgca ggccagcgtc gacagccggc
     3721 gcgacctcca cctcgtaccc gtggtcggtc aggatgtcgc gcagcaaatc aagcgtctcg
     3781 tggttgtcgt ccactaccgc gacgcgtcct cggctcatgg cgcgatagcc gtatgcaagt
     3841 tggcggccag tgctggccgg gcattcgaac cggcttgctt tcccgacagt gagcgtggga
     3901 tgtggagcgc cgctgcatcg gtccacgtgc aatgccgagg atgtggataa ctctgcgggc
     3961 atatgtggaa aaaccggcaa cggcgctcgg accggctcgc tagaatcggt caaacccgcg
     4021 gcgagacggg ttgacaagtc aacacccacc gcgcagggag atcccttcga tgccgcttcg
     4081 gtcggacgca gagcacttcg cactcatcaa ggtcatcggc gtcggaggcg gcggcagcaa
     4141 cgccgtgaac cggatgattc gcgccgagat gatgggcgtc gagttcatcg gcgtgaacac
     4201 cgacgcccag gccctcctcc agagtgatgc gccgcacaag atccgcatcg gcgacaagat
     4261 gacccgcgga ctgggcgccg gggccgaccc gcaggtcggg cagcgggcgg ccgaggagga
     4321 ctcggagaag atctacgagg ccctcaagga cgccgacatg atcttcatca ccgccggcat
     4381 gggcggcggc accgggtcgg gcgcggcccc ggtcatcgcc gagatcgcca aggacctggg
     4441 tgcgctgacg gttggcgtcg tcaccaagcc attcagcttc gagggcatgc gccgcaagct
     4501 ggtggccgag cagtacaccg agctgctcaa ggagaaggtg gacaccctga tcaccatccc
     4561 caacgaccgg ctgcgcgagg tcgtggacaa gaagacgtcc atcgtggacg ccttccgggt
     4621 ggtggatgac gtgctgcgcc agggcgtgca gggcatcagc gacctgatca ccgtgcc
//
LOCUS       DATACA010000433         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_58129, whole genome shotgun sequence.
ACCESSION   DATACA010000433 DATACA010000000
VERSION     DATACA010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58129"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(353..730)
                     /locus_tag="VHK28_08835"
     CDS             complement(353..730)
                     /locus_tag="VHK28_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2142358.1"
                     /translation="MNRTRSILLALGLMLALAACSAATGGDATPLPDADVTVVAEDMK
                     FDTDTLTLPAGQPFTLLLENRDGAPHNVAIYTDSSASEELYVGETVSNGAIVYEIDAL
                     EPGTYYFRCDLHPDMNGTVVVEA"
     gene            complement(807..1250)
                     /locus_tag="VHK28_08840"
     CDS             complement(807..1250)
                     /locus_tag="VHK28_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142359.1"
                     /translation="MTALPRNLSRPTGARAFGVALTAAIVGLALTTAYIHLGLGGLLF
                     TLNGLGYAGLAAIYLLGAAAPHPLARTFSWAPRVALIGYAALTIVAWAIEGPYFDLAY
                     ITKAVEVTLIALVVVDIQRVYGGVPSMVRVARASIFGIGSRPSAR"
     gene            1459..>3233
                     /locus_tag="VHK28_08845"
     CDS             1459..>3233
                     /locus_tag="VHK28_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2142360.1"
                     /translation="MTAESELLQSAVREVARLLQVDGAMIYLVEPTTGELRFAHDAGI
                     TNPEARRLIHGLTLPVGVGMFGTAVASRHVVVTDDYPADRRFSHSEVADRIVQVAGMR
                     SMAVAPLIAGDEPLGALGAYSRRPAAFNEAQTVLLRALAEHAATAIANQRLVLKLAAS
                     EERYRTQTLELAHRVATQQTLHRIASQITAIHDPDEALQQVVDGAKRLLASDGAHLTL
                     RDPEQPMLRPHVMAGGTDAETSAWMAVQEFPIGGGMNGLAAELGEPVWTYDYLVDPRI
                     PHTDDDQRVAGRMGLRGMAVAPLRAPGGEIIGTLAVSHERPRETTSDELELLQGLADI
                     GAIAIANARLYGQLTRSEEDLRQQAGELERVVEVQRTLGEIARRIVEVDGAAETLQQV
                     VDASKRLLGSDGAHLTLMSDDGEYLIPMVLAGDESPERRSWLHTQRFPVGGGINGLAA
                     ASGQPAWTDDYANDERIPHDPDDDAPARLELGAVAVAPLRGVRGDVIGTLAITYRKPR
                     HIDPRDVALLEELAGQGAIAARNAQLYQQLRERADALARSAEAQRTLAAIAAQISSIR
                     DPGTVLRRTVDESKRLLGADHAVIDR"
BASE COUNT          493 a         1120 c         1165 g          455 t
ORIGIN      
        1 gggctggcga gccgcaccca ggccgccctg tgggcggttg agcacaagct ggtcgagccc
       61 gctccctgac ggcaggcacg agagaggcgc ccggcccgat gcggaccgga cgcctcgccc
      121 taaggggtca cctgctgtga aataggcccg ttcggccggt cgcgacgacg acgcgccctt
      181 ccaagcgtcg aatttcacgc gaggtgtgaa atattcggct gcaaaggctc ccgagcggca
      241 ctaccggcca cttggtgtga attcggtagc ttcgaaggct ccacggcgac cctgccggtc
      301 accaggtgtg aatccgtccc gttcgggcct gttcggaagc tcccccgggg agctacgcct
      361 cgaccaccac cgtgccattc atgtcggggt gcaggtcgca gcggaagtag taggtgccag
      421 gctccagcgc gtcgatctcg tacacgatcg cgccgttgct gaccgtctcg cccacgtaca
      481 gctcctccga cgccgagctg tcggtgtaga tcgccacgtt gtgcggcgcg ccgtcccggt
      541 tctccagcag cagggtgaac ggctgcccgg cgggcagggt cagggtgtcg gtgtcgaact
      601 tcatgtcctc ggccaccacc gtgacgtcgg cgtccggcag gggcgtcgcg tcgccgccgg
      661 tcgccgccga acaggcggcc agagccagca tcagccccag ggccagcagg atggatcgtg
      721 tgcggttcat cggtcccctc gttgtcgtca ggtcttgatg ggtgatggac cgatgggctg
      781 gcgggcccat cggtcctgcg cgtggctcag cgggcggacg gccgcgagcc gatgccgaag
      841 atggaggcgc gggcaacgcg gaccatggac ggcacgccac cgtagacgcg ctggatgtcc
      901 acgaccacca gcgcgatgag cgtcacctcg acggccttgg tgatgtaggc caggtcgaag
      961 tacggacctt cgatggccca ggccacgatg gtcagcgctg cgtagccgat cagggccacg
     1021 cgcggtgccc agctgaaggt gcgggccagc gggtgcggag ctgcggctcc cagcaggtag
     1081 atggcggcca ggccggcgta gcccaggccg ttgagggtga agagcagccc gcccaggccc
     1141 aggtggatgt aggccgtggt gagcgccagt ccgacgatcg ctgcggtcaa cgcaacgccg
     1201 aaggcgcggg cgccggtggg acggctgagg ttgcggggaa gcgcggtcat ggttggatgt
     1261 ctcccatcgg tgattcgctc gcgccgctct gtgtgaatta gcggcgttcg taatggaaga
     1321 cacgctaggt gccattgatg tgaccctcat ccggcagccg cctccacccg gaccggatat
     1381 gcgccccgct cgcggccgga cgatccgccg cctggccgat cggccatctg acgtacggcg
     1441 gggtatgctc acgcttccat gacggccgaa tccgagctcc tccagagcgc cgtccgggaa
     1501 gttgcccgcc tcctccaggt ggacggggcg atgatctacc tggtcgagcc caccaccggt
     1561 gagctgcgat tcgcgcacga cgcggggatc accaacccgg aggcacggcg cctgatccac
     1621 ggcctcaccc tgccggtcgg ggtgggcatg ttcggcacgg ccgtcgccag ccgccacgtg
     1681 gtggtcaccg atgattaccc cgcggaccgg cggttcagcc actccgaggt ggcagaccgt
     1741 atcgtgcagg tggcaggcat gcgatccatg gcggtcgcac cgctgattgc cggcgacgag
     1801 ccgctcggcg ccctgggcgc ctactcgcgc cgaccggccg ccttcaacga ggcgcaaacc
     1861 gtactgctgc gcgccctcgc cgagcatgcg gccaccgcca tcgccaacca gcggctggtg
     1921 ctcaagctgg cggcctcgga ggagcgctat cggacccaga cgctcgagct ggcgcaccgg
     1981 gtggcgaccc agcagacgct gcaccgcatc gcctcgcaga tcacggccat ccacgacccg
     2041 gacgaggcgc tgcagcaggt cgtggacggg gccaagcggc tgctggcttc ggacggcgcg
     2101 cacctcaccc tgcgggaccc ggagcagccc atgctgcggc cgcacgtcat ggccggaggc
     2161 acggatgccg agaccagcgc ctggatggcg gtccaggagt tcccgatcgg cgggggcatg
     2221 aacggcctgg cggccgagct gggtgagccg gtgtggacct acgactacct ggtcgatccg
     2281 cgcatcccgc acaccgacga cgaccagcgg gtcgccgggc gcatgggcct gcgcggcatg
     2341 gccgtggcgc cgctgcgcgc cccgggaggg gagatcatcg gcaccctggc cgtctcgcac
     2401 gagcgtccgc gcgagaccac cagcgacgag ctggagctcc tccaggggct cgccgacatc
     2461 ggcgccatcg ccatcgccaa cgcccgcttg tacgggcagc tcacccgctc ggaggaggac
     2521 ctgcgccagc aggcagggga gctggagcgc gtggtcgagg tgcagcgcac gctgggcgag
     2581 atcgcgcggc gcatcgtgga ggtggacggt gcggccgaga cgctgcagca ggtggtggac
     2641 gcctcgaagc gcctcctcgg ctccgacggc gcgcacctga ccctgatgag cgatgacggc
     2701 gagtacctga tccccatggt cctggccggc gacgagagcc cggagcggcg gtcctggctg
     2761 cacacccagc gctttccggt gggtggcggc atcaacggcc tggcggccgc ctcgggccag
     2821 ccggcctgga ccgatgacta cgccaacgac gagcgcatcc cgcacgaccc ggacgacgat
     2881 gctccggcgc ggctcgagct gggcgcggtg gcggttgcgc cgttgcgcgg cgtgcgcggc
     2941 gacgtgatcg gcacgctggc catcacctac cgaaagccac gccacatcga cccgcgggac
     3001 gtggcgttgc tcgaggagct ggccgggcag ggggcgatcg ccgcgcgcaa cgcccagctg
     3061 taccagcagc tgcgcgagcg ggcggatgcg cttgcccggt cggcggaggc gcagcgcacg
     3121 ctggccgcca ttgccgccca gatcagctcc atccgcgacc ccggcaccgt cctgcgtcgc
     3181 acggtggacg agtcgaagcg cctgctcggc gcggaccacg ccgtcatcga ccg
//
LOCUS       DATACA010000434         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_459687, whole genome shotgun sequence.
ACCESSION   DATACA010000434 DATACA010000000
VERSION     DATACA010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459687"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(802..1263)
                     /gene="smpB"
                     /locus_tag="VHK28_08850"
     CDS             complement(802..1263)
                     /gene="smpB"
                     /locus_tag="VHK28_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX2142361.1"
                     /translation="MSDTTVALNRRARHDYTIEDTLEAGLVLTGTEIKSIRAGRVNLA
                     EAYARIERGEAWLIGAHIAPYEQGNRNNHEPTRTRKLLLHRDQIAELVGKSQAKGYTL
                     IPLRLYLRGGRAKLELGIGRGKKAHDKRRAIAERDMRREVQREAKDARVKL"
     gene            complement(1477..2559)
                     /locus_tag="VHK28_08855"
     CDS             complement(1477..2559)
                     /locus_tag="VHK28_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX2142362.1"
                     /translation="MPPEDYRRALGDLSGEFSGIGAEMGVRNLQDPDDLEACTTFSDI
                     CALVVVAPLDDSPAERAGLRAGDVVLAVDGEPVNGIDLSEAVQRIRGEAGTDVTLTLR
                     RGEETLELTITRAEIELREVTSRMVEPGIGYIALNGFSEPAASQFRDALSALIDDGAT
                     SIVFDLRDNPGGYIDAARRIASEFIEEGVLFTQESSGDEVRTWEATGDGVATDSDIEV
                     VVLVNGGSASASEIVAAALKERERATVVGQPTYGKNTVQVWNDLDNGGGVRITISRWF
                     TPDHNSVAPDGVQPEVVVEVPEGTPPERDLFLETALEVLQDRAVGDDEPGESQPASGL
                     RLPPLAWTDAFEPIGWDPGGLLRARA"
BASE COUNT          397 a          924 c          883 g          435 t
ORIGIN      
        1 tacgaggcca gcgggcgcct cgacctgcag tttccggatc atgagccctg tcgaaacctg
       61 acatccccac ggatccgctt gttggaccga tgataccacg gcccgctccg aatgtgaaca
      121 cctgtgcgaa atggaggatc agccctccgt catctccagc cagcgcgggt tccagggccc
      181 ctgcccgtag atctgctcga tctccatgcg gaacacccag ccggtgggcc atcccggtac
      241 ggatacttgg cgcctggacc gatgtagacc tcgctgagcc gatccatcac ctccagcgcg
      301 ccgctttccg tgacccgagc cgtgccgctg atcaccacgc acgggtacag gcccggtccc
      361 tcgtaggcct tcgcctggaa cgatgccgtc acccacgggt tgcgcttcac gcgcgcgacc
      421 cgcttcgggt cgaagaaggt gccgaagacg agctcgtcgc cgtcgagacc ggccaggaga
      481 gctggacgtg acgctgaccg ttcgagcgcg tgatgatgag gtgggcgaac gccccggtcg
      541 cgagcaggtc acgcaccgcc tgcgaagtca ttgccatagc aaccatgtta cgcctcgatg
      601 actgcgttct cctcggattc agatgaggtg cgcatgtcga acggtgattc ccgcatctag
      661 cgccaccgtg caggatgacg gccgcatctg cgccacgcag cccccaaacg gatccccgtg
      721 ccggcctcac ctgaatctgg cgagccttgt ccggagtccg aacgccgggg acctagccac
      781 ctggcgcgac ctgctacacg gtcagagctt gacgcgagcg tccttcgcct cgcgttgcac
      841 ctcgcggcgc atgtcgcgct cggcgatggc gcggcgcttg tcgtgcgcct tcttgccacg
      901 accgatgccc agctccagct tggcccgccc gccgcgcagg tagaggcgca gggggatgag
      961 cgtgtaaccc ttggcctgcg acttccccac cagctccgcg atctggtcgc gatggagcag
     1021 cagctttcgg gtgcgggtgg gctcgtggtt gttgcggttg ccctgctcgt acggcgcgat
     1081 gtgcgcgccg atcagccagg cctcgccgcg ctcgatgcgg gcgtaggcct cggccaggtt
     1141 gacgcggccg gcgcggatcg acttgatctc ggtcccggtc agcaccaggc cggcctcgag
     1201 ggtgtcctcg atggtgtagt cgtggcgcgc gcgacggttg agggcgacgg tcgtatcgga
     1261 catcggtgag tgcatcctgg tgagctggcg ccggacatgg agagagccgg cccgtctcgg
     1321 ccggctcctc cgacgcaacc actcgaagag gtgagttagc gcaccgcctc ggtggtggcg
     1381 tgacaggaac tactcactgc acaccacctc ctttcgttga gaccgatgct aacagccccg
     1441 ggcggcacaa cgcaacgccc cctttcagga ggcacgtcag gcgcgggcgc gcagcagtcc
     1501 tccggggtcc cagccgatag gctcaaaggc gtcggtccag gccagtggcg gcaggcggag
     1561 ccctgatgcg ggctggctct cgcctggctc atcgtcgccg acggcgcggt cctggagcac
     1621 ctccagcgcg gtctccaaaa agaggtcgcg ctcgggcggc gtgccctccg gcacctccac
     1681 gaccacctcg ggctgtacgc catccggcgc gacgctgttg tggtccggcg tgaaccaccg
     1741 agagatcgtg atccgcaccc cgccgccgtt gtccaggtcg ttccagacct ggaccgtgtt
     1801 cttgccatag gtcggctggc ccaccaccgt cgcccgctcg cgctccttga gggcggcggc
     1861 cacgatctcg gacgcggatg ccgagccgcc gttgacgagt accacgacct cgatgtcgga
     1921 atcggtggcc acgccgtccc cggtcgcctc ccaggtgcgt acctcgtcgc cggacgactc
     1981 ctgggtgaag agcacgccct cctcgatgaa ctcggaggcg atgcggcgag ccgcgtcgat
     2041 gtagccgccc gggttgtcgc gcaggtcgaa gacgatggag gtggcgccgt catcgatcag
     2101 ggcggagagc gcgtcgcgga actggctggc ggccggctcg gaaaagccgt tgagcgcgat
     2161 gtagccgatc cccggctcca ccatccggct ggtgacctcg cgcagctcga tctcggcgcg
     2221 ggtgatggtc agctccaggg tctcctcgcc gcgacgtagg gtgagggtca cgtccgtgcc
     2281 ggcctcgccc cggatgcgct gcaccgcctc cgacaggtcg atgccgttga ccggttcgcc
     2341 gtcaacggcc agcaccacgt cgccggcgcg cagcccggcg cgctccgccg gggagtcgtc
     2401 cagcggcgcc acaacaacca gcgcgcagat gtcgctgaag gtggtgcagg cctcgaggtc
     2461 gtcggggtcc tgcaggttgc gcacgcccat ctcggcgccg atgccgctga actcgccgga
     2521 caggtcaccg agcgcgcgcc ggtagtcctc tggcggcatg ttcgagtacg gcgtgcgcga
     2581 cccgttcagc ggctacatgc cgccagagga ctaccggcgc gcgctcggcg acctgtccg
//
LOCUS       DATACA010000435         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_58518, whole genome shotgun sequence.
ACCESSION   DATACA010000435 DATACA010000000
VERSION     DATACA010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58518"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VHK28_08860"
     CDS             <1..1073
                     /locus_tag="VHK28_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX2142363.1"
                     /translation="VVMNPQNGEILAMVSLPAYDNNKFATGISAQDYSVYLEDPAKPL
                     RNHAIADIFAPGSTFKLVTGLAALQEGVTSAARRWPTYGCYQIPGAPAGQCLFDWNRQ
                     GFGPLDMIDAFAVSSDTYYYQMAIALGVDALGAWANELGFGERTGVALPGEATGIIAS
                     RAWARSQGRENLFTGELAQAGIGQNVVAVTPIQLLNAYAALANGGTLWRPQIVRGETD
                     ADGQLVREYEPEAIRQLAADPANLQTMRIGAREVITTGHAYNIRDLDLPGALSGKTGT
                     AEFGSPTEEGTLPFHSWFVAYLPSSAGATDAELAVVTFSYSAVVPGNVSLEVVKYFLQ
                     EWYDLDQDLRLDPRDFSLVSAN"
     gene            1077..>1959
                     /locus_tag="VHK28_08865"
     CDS             1077..>1959
                     /locus_tag="VHK28_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEX2142364.1"
                     /translation="MGATTLTSTLVDGSRIRRWRAFDGQLFLYMVLLIALGVVVGYSA
                     SFSEAAAGAGLSQTVKTLIWAAIGLTIFFVVASVDYHWLQTLTVPIYLIVLGLLTLTM
                     FVGTNLFGAQMSITVAGLDFQFSEISKVLMIVVLASFLSSRRERIGRLSTIVGAGVLM
                     AIPTFLVFRQPDLGTALVFVAILVGMLFMSGASIGWMGIFAGISVAIGPLVVQSLQDY
                     QRQRLFCFLDPSADPQGACYQLIQSLNAVGSGGLFGQGLTAGRQNQLGFLPVQSTDFI
                     FTVVAEELGFVGGVLTLG"
BASE COUNT          282 a          691 c          653 g          333 t
ORIGIN      
        1 ccgtggtgat gaacccgcag aacggcgaga tcctggccat ggtctcgctc cccgcctacg
       61 acaacaacaa gttcgccacc gggatcagtg cccaggacta cagcgtctac ctcgaggacc
      121 cggccaaacc gctgcgcaac cacgccatcg ccgacatctt cgcgccgggc tccaccttca
      181 agctggtgac gggactggcg gcgctccagg agggagtcac ctcggccgcg cgccgctggc
      241 ccacctacgg ttgctaccag atcccgggcg ccccggccgg ccagtgcctc ttcgactgga
      301 accgtcaggg cttcggcccg ctggacatga tcgacgcatt cgcggtcagc tcggacacct
      361 actactacca gatggcaatc gccctggggg tggacgcgct cggcgcctgg gcgaacgagc
      421 tcggcttcgg ggaacgcacg ggcgtggcgc tgccgggcga ggctacgggc atcatcgcca
      481 gccgcgcctg ggcccgctcg cagggccgcg agaacctgtt caccggtgag ctggcgcagg
      541 cgggcatcgg gcagaacgtg gtggccgtca cgcccatcca gctgctcaac gcctacgcgg
      601 cgctggccaa cggcgggacc ctgtggcggc cgcagatcgt gcgcggcgag acggacgcgg
      661 atggccagct ggtgcgcgag tacgagccgg aggcgatccg ccagctggcc gccgacccgg
      721 ccaacctgca gaccatgcgc atcggtgccc gggaggtgat caccaccggc cacgcctaca
      781 acatccgcga cctggacctg ccgggcgccc tgtcgggcaa gacgggaacc gccgagttcg
      841 gctcgccgac cgaggagggc accctcccct tccactcctg gttcgtcgcc tacctgccct
      901 cgtccgccgg tgcgaccgat gccgagctgg cggtggtcac cttcagctac tcggcggtgg
      961 tgcccggcaa cgtgtcgctt gaggtggtca agtacttcct ccaggagtgg tacgacctcg
     1021 accaggatct gcgcctggat ccgcgggact tcagcctggt ttcggcgaac tgaccgatgg
     1081 gcgccacgac cctcacctcc accctggtcg acggatcgcg catccgccgc tggcgcgcgt
     1141 tcgacgggca gctgttcctg tacatggtgc tgctcatcgc gctgggcgtg gtcgtcggct
     1201 actcggcgag cttcagcgag gccgccgcgg gggcggggct gtcgcagacg gtcaagaccc
     1261 tgatctgggc cgccatcggc ctcaccatct tcttcgtggt ggccagcgtc gactaccact
     1321 ggctccagac gctgacggtg ccgatctacc tgatcgtgct ggggctgctg acgctcacca
     1381 tgttcgtggg caccaacctg ttcggcgccc agatgtcgat caccgtggcc ggcctggact
     1441 tccagttcag cgagatcagc aaggtgctga tgatcgtggt gctggcctcg ttcctgtcct
     1501 cgcgacggga gcgcatcggg cggctgtcga ccatcgtggg agccggcgtc ctgatggcca
     1561 tccccacctt cctggtcttc cggcagccgg acctcggcac ggcgctggtg ttcgtggcca
     1621 tcctggtcgg catgctcttc atgagcggcg ccagcatcgg ctggatgggg atcttcgcag
     1681 gcatcagcgt ggcgatcggc ccgctcgtgg tccagtcgct gcaggactac cagaggcagc
     1741 gcctgttctg cttcctggat ccatcggccg atccgcaggg cgcttgctac cagctgatcc
     1801 agtcgctcaa cgccgtgggc tccggcggcc tgttcggcca gggcctgacc gccggccggc
     1861 agaaccagct cggcttcctg ccggtgcagt ccaccgactt catcttcacc gtggtcgccg
     1921 aggagctggg gttcgtgggc ggggtcctga ccctcggca
//
LOCUS       DATACA010000436         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_539284, whole genome shotgun sequence.
ACCESSION   DATACA010000436 DATACA010000000
VERSION     DATACA010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539284"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1387
                     /locus_tag="VHK28_08870"
                     /pseudo
     CDS             134..1387
                     /locus_tag="VHK28_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            1598..2350
                     /locus_tag="VHK28_08875"
     CDS             1598..2350
                     /locus_tag="VHK28_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX2142365.1"
                     /translation="MASQPVNEPTDAAAEYLMTIRYMHGEGQPVIAARLAERLRVSPA
                     TVSEMVNRLAREGLLTVDEATRQLELTDAGRTTAERTFRRHALAEWLLTEVVGLGWAE
                     ADEEAHHLQHALSERVTDRIDELLGEPPTCPHGNPIPRDGQTPDRPAGMPLSQAAAGG
                     EITVLRVTEEAEEDARLLTFLQEHAVRPGSVFRVREVAEHIGTMTLDGPMGDVTLGLT
                     AAAKIRALPGRADPGLFHRVPERARLATAAAG"
     gene            2367..2573
                     /locus_tag="VHK28_08880"
     CDS             2367..2573
                     /locus_tag="VHK28_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142366.1"
                     /translation="MTRARTRRTIIASVGTVLWLGYMVVGLALIDTYLPTTGGEGPEG
                     LAAFVGLIWGVVVCGLIMWAASED"
     gene            2591..2824
                     /locus_tag="VHK28_08885"
     CDS             2591..2824
                     /locus_tag="VHK28_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142367.1"
                     /translation="MHQFQDGLAVRRPSLDDAQRIHDLLVACDMHDFGSVDFSADETR
                     DELSRTDLENDAWLVESDGSPVGFAGVQLRGGV"
BASE COUNT          471 a         1005 c         1019 g          421 t
ORIGIN      
        1 cgaggtgtag agcaggtaca ggaggtcctc ggagtccatc ggctccgggt ccgcggtggt
       61 cgcctgcgac tcgcggagct catgccacca gtggtcgcgc ccatcggtcc agtcaaccgg
      121 atcgcccgtg cggcgcgacc actggtggca tgagctccgc gagtcgcagg cgaccaccgc
      181 ggacccggag ccgatggact ccgaggacct cctgtacctg ctctacacct cggggaccac
      241 cgcccggccg aagggcatca tgcacaccac cggcggctac ctggttggca ccgccaccac
      301 ccatcggtac atcttcgaca tccgaccgga gaccgacgtc tactggtgca tggccgacat
      361 cggctgggtg accgggcact cgtacatcgt gtacgggccg ctggccaatg ccagcaccgg
      421 gatcatctac gagggcgcca tcgacttccc ggacaaggac agatgggccc agatcgcggc
      481 gaagtaccgc gccaccatcc tctacaccgc tccgaccgcc atccgggccc tgatgaagta
      541 cggccacaca gagttcgagc gggacgacct gtcgtcactg cgcctgctgg gcagcgtcgg
      601 cgagccgatc aacccggagg cctgggcctg gtactggcga tacgtcggcg gcgaacgctg
      661 cccggtggtc gacacctggt ggcagaccga gacgggcatg atcatgatca ccccgctgcc
      721 aggcatcacc accctgaagc cgggcagcgc gaccttcccg ttccccgggg tgaaggcgga
      781 cgtggtggac gaggcgggca actcggtgcc actcgggcgt ggcggctacc tggtgctcga
      841 gcggccgtgg ccggccatgc tgcgcggcat ctacggcgat ccagagcgct acgagcagac
      901 ctactggagc cggtacccgg gacgctactt cgccggcgac ggctgcaagc gcgacgacga
      961 cggctactac tggctgctgg gccgagtgga cgacgtgatg aatgtctcgg gccatcgtat
     1021 ctcgaccacc gaggtggagt cggcgctggt ctcgcacgag gccgtggcgg aggccgcggt
     1081 ggtgggccga acggacccca tcaccggcca gggcatcttc gcctacgtga tcctgcgctc
     1141 cgggatcgag cccgccgacc cgctggcagc cgagctgcgc gagcacgtgg ccgacatgat
     1201 cggcaagttc gcgcggccca agcagatcat gttcacgccg gacctgccca agacgcgctc
     1261 cggcaagatc atgcggcggc tgctgcgcga tatcgccgag cagcggacgc tgggcgacgt
     1321 gaccacgctg gccgacagca acgtggtcaa caccatccgc gagcagagcg gcaccggcga
     1381 ggagtaggcg ccgacagatc gggcagttct tcacatgagc gcgctgctgc gagcgattct
     1441 gctgttgtag cgggcagccc tgagcgtggc gggccaggaa cttgtcgggt catgcgtgcg
     1501 gctgcccgct acatcggcgg tttgacccct cacgagccct ccgcgtgaaa tccggcccga
     1561 aatatcggcg ccccgacgac gccactagaa tcggtccatg gccagccaac cggtgaacga
     1621 gccgaccgat gcggccgccg agtacctgat gaccatccgc tacatgcacg gcgaggggca
     1681 gccggtgatc gccgcgcgcc tggcggagcg gctgcgggtc agccccgcca cggtgtcgga
     1741 gatggtcaac cggctggccc gcgagggcct gctcaccgtg gacgaggcca cgcggcagct
     1801 ggagctgacc gatgccggtc ggaccaccgc ggaacgcaca ttccgccgcc acgccctcgc
     1861 cgaatggctg ctcaccgagg tggttgggct gggctgggcc gaggcggacg aggaggcgca
     1921 ccatctgcag cacgctctgt ccgagcgggt gaccgaccgc atcgacgagc tgctgggcga
     1981 gccaccgacc tgcccgcacg gcaacccgat cccgcgtgac gggcagacgc cggaccgccc
     2041 ggccggcatg ccgctgtcgc aggcggccgc cggtggcgag atcaccgtcc tgcgcgtcac
     2101 cgaggaggca gaggaggacg cacgcctgct gaccttcctg caggagcatg ccgttcgacc
     2161 cgggagcgtc ttccgtgtgc gcgaggtggc ggagcacatc ggcaccatga ccctggatgg
     2221 accgatgggc gacgtgaccc tgggcctgac cgccgccgcc aagatccgcg cccttcccgg
     2281 acgcgccgat cccggcctgt tccaccgggt gccagaacgg gcacggctgg cgaccgcggc
     2341 ggccgggtga tgctaggatc tcggccgtga ccagggcgcg cacccgccgc accatcatcg
     2401 cctcggtcgg caccgtcctg tggctcggct acatggtggt cgggctggcc ctgatcgaca
     2461 cgtacctgcc caccaccggt ggagaaggcc cggagggcct ggccgcattc gtgggcctca
     2521 tctggggcgt cgtcgtatgc gggctcatca tgtgggcagc ctccgaggac tgacgtcccg
     2581 gagccccggc atgcaccagt tccaggacgg cctggccgtg cgccgtccgt cgctggacga
     2641 cgcgcagcgc atccacgacc tgctggtcgc ctgtgacatg cacgatttcg gctccgtcga
     2701 cttcagcgcg gatgagacca gggacgagct gtcgaggacg gacctcgaaa atgacgcctg
     2761 gcttgtcgag tcggacggca gtccggtcgg cttcgccggg gtccagctgc ggggcggcgt
     2821 gtagcatcgg gcgcaggtat cggtccatcc cggttggcga gagcgcggta tcggttcctg
     2881 gctggcgaac agtgctgtgg cccgagctcg ggagcg
//
LOCUS       DATACA010000437         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1504827, whole genome shotgun sequence.
ACCESSION   DATACA010000437 DATACA010000000
VERSION     DATACA010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504827"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHK28_08890"
     CDS             complement(<1..192)
                     /locus_tag="VHK28_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEX2142368.1"
                     /translation="MSPPTPRRTSSQDPFAALAGRPVLSAVLGALAIAFSGVLVRQAD
                     VAPATAAIFRCLYALPPLAL"
     gene            267..>2612
                     /locus_tag="VHK28_08895"
     CDS             267..>2612
                     /locus_tag="VHK28_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142369.1"
                     /translation="MRSLPTGTVTFLFTDIEGSTRLAQALGEAWPPILERHNAVLREA
                     VEGNDGVVFGTEGDALFAAFGSASDAVRASVAAQRALAAEPWPEGATVRVRMGIHTGE
                     ARMAGDDYAGIEVHRVARISNTGHGGQIILSGPTAMLVEGALPAGVSVRDLGEHRLKD
                     LSRPELLKMLVADGLPDGFPPLRTLDAVPNNLPTQLTTFLGRDRELAEASALLLQARL
                     LTLTGPGGSGKTRLALQMAAEASDSFPDGVYFVPLGPLDNATLVLPTIAQAMGLPNPG
                     GQALDRLGEHLAGRRVLFVLDNFEQVLDAASDIAGLLERLPDSTALVTSRSPLRVYGE
                     QEYPVPPMDVPDPHHLPDLESLSHYASVALFVERAMAVKPSFRVDASNAPAIAEICAR
                     LDGLPLAIELAAARVRVLTPEAIMARLDRRLSLLAGGSRNLPERQQTLRGAIDWSYDL
                     LDDADRLVFARFSAFAGGADVPAVEGVVLADWPADGGQAPDALDALTALLDKSLVRQE
                     MTPDGESRFRMLETIREYAAERLAESEPDADTRLRHAEYYAAVAEGVAARIFGDEQRA
                     CLDALEREHDNLRAAIGFALEQRRTELGLRLLAASWRFWQMRGYLPEGRDRAERVLAL
                     PDTASHPEQLLRALDAAGGIAYWQGDMVRAREWYAAEREVARQLDDRPAEAEAAYNQS
                     MAYAFREDVNESLQLGTEAIELFRALRDRHGEARAMWGASSAMAMGRMVDEGMPLLEK
                     AEEVFREVDDRFLLPWALYVKSLFLIQQRELDAARAALEESI"
BASE COUNT          372 a          924 c          946 g          370 t
ORIGIN      
        1 cagggcaagc ggcgggagcg cgtacaggca gcggaagatg gcagccgtgg ccggcgccac
       61 gtccgcctgg cgcaccagca cgccgctgaa ggcgatggcc aatgccccga gcacggcgga
      121 cagcaccggc cgtccggcca gggcggcgaa ggggtcctgc gaacttgtgc gacgcggtgt
      181 cggcgggctc acgactgcgg agcgtagcgc gaccggccct tccgggggct tgccctgcgg
      241 gccgctcacc cgcgaaactt gccggcgtgc gaagcctgcc gaccgggacg gtcaccttcc
      301 tgttcaccga tatcgagggg tccacgcgcc tcgcgcaggc cctcggtgag gcctggccgc
      361 cgatcctgga gcggcacaac gccgtgctgc gcgaggccgt cgagggcaac gacggcgtgg
      421 tcttcgggac ggagggcgac gcgctgttcg ccgccttcgg cagcgcgtcg gatgccgttc
      481 gcgcctcggt ggcagcgcag cgcgccctcg ccgcggagcc ctggccggag ggcgccaccg
      541 tccgcgtgcg gatgggaatc cacaccggcg aggcgcggat ggccggcgac gactacgccg
      601 ggatcgaggt gcaccgcgtg gcgcgcatct cgaacaccgg tcacggcggt cagatcatcc
      661 tctccggccc caccgcaatg ctcgtcgaag gcgccctgcc ggcgggagtg agcgttcgtg
      721 acctcggcga gcaccggctc aaggacctgt cgcggcccga gctgctgaag atgctggtgg
      781 ccgatggcct gccggacggc ttcccgccac tgcgcacgct ggacgcggtt cccaacaacc
      841 tgccgacgca gctcaccacc ttcctggggc gtgaccgcga gctggcagag gcgtccgctc
      901 tgctgttgca ggcacggctg ctgaccttga ccgggccggg cggctcgggc aagacccggc
      961 ttgcactcca gatggcggcg gaggccagcg actcgttccc cgacggcgtg tacttcgtgc
     1021 cgctcggacc gctggacaat gcgacgctgg tcctgccgac catcgctcaa gccatggggt
     1081 tgccgaaccc cggcggccag gccctcgacc gcctggggga gcaccttgcc ggccggcgcg
     1141 tcctgttcgt cctcgacaac ttcgagcagg tgctcgatgc ggcctcggac atcgccgggc
     1201 tgctggagcg gcttcccgat tcgacggcgc tggtcaccag ccgctcgccg ctgcgggtgt
     1261 acggcgagca ggagtacccg gtcccgccca tggacgtccc cgacccgcac cacctgccgg
     1321 acctggagtc gctgtcgcat tacgcgtccg tggccctgtt cgttgaacgc gccatggcgg
     1381 tcaagcccag cttccgggtg gatgcctcca acgccccggc catcgccgag atctgcgcgc
     1441 ggctggatgg cctgccgttg gccatagagc tggccgccgc ccgggtgagg gtcctgactc
     1501 cggaggcgat catggcgcgg ctcgaccgtc gcctctcgct gctggccggt ggctcacgca
     1561 acctgccgga gcggcagcag acgctgcgcg gcgccatcga ctggtcgtac gacctcctgg
     1621 acgacgcgga tcgcctcgtg ttcgcgcgct tcagcgcctt cgccggcggc gccgacgttc
     1681 cggcggtcga gggcgtggtg ctcgccgact ggccggcgga tggagggcag gcgcccgacg
     1741 ccctcgacgc gctcaccgcc ctgctggaca agagtttggt tcgccaggag atgacccctg
     1801 acggcgaatc ccgattccgg atgctggaga ccatccgcga gtacgccgcc gagcgcctgg
     1861 ccgaatccga gccggatgca gacacgcgcc tgcgccacgc cgagtactac gcggcggtcg
     1921 ccgagggggt cgcggcgcgc atcttcgggg acgagcagcg tgcctgcctc gacgccctgg
     1981 agcgggagca cgacaacctg cgcgccgcca tcggctttgc gctggagcag cggcgcaccg
     2041 agctcggcct tcggctgctg gccgcaagct ggcgcttctg gcagatgcgc ggctacctgc
     2101 cggagggcag ggaccgtgcc gagcgggtgc tggccctgcc ggacacggcg tcccacccgg
     2161 aacagctgct tcgcgcactg gatgccgccg gaggaatcgc ctactggcag ggggacatgg
     2221 tgcgggcgcg cgagtggtac gcggccgagc gagaggtcgc gcggcagctc gacgaccgcc
     2281 cggccgaggc cgaggcggcc tacaaccaga gcatggcata cgcgttccgc gaggacgtga
     2341 acgagtccct gcagctcgga accgaggcaa ttgagctatt cagggcactg cgcgaccgcc
     2401 acggcgaggc acgcgccatg tggggcgcgt ccagcgccat ggccatgggc cgaatggtcg
     2461 acgaaggaat gccgctgctg gagaaggcag aggaagtctt ccgcgaggtc gatgaccggt
     2521 tcctgctccc atgggccctg tacgtcaagt ccctcttcct gatccagcag cgggagctcg
     2581 atgccgctcg cgccgcgctg gaggagtcca tc
//
LOCUS       DATACA010000438         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1745670, whole genome shotgun sequence.
ACCESSION   DATACA010000438 DATACA010000000
VERSION     DATACA010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745670"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VHK28_08900"
     CDS             complement(<1..904)
                     /locus_tag="VHK28_08900"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142370.1"
                     /translation="MVEEGAARHLELAEEALRAFDFDAAVAHLSGAIRTLTAAGERRQ
                     AALTCARLGDLYANAIGNRTAARAWFLRATRLIEDEPPCLEQGWVAVAAMGCDVDDPA
                     VLLARAELALARARQFGDVNLETKALADGGLACVQVGRVAEGMAMLDEAMALACGPAD
                     NLDAAGKSVCSFFTACYFAADFDRAGSWADALRRQGLIGKAPGAPLYLSSHCDSVQAT
                     ALCELGRWGEAEAMLTRAIEDFEAGMGMPSWHPAIALAELRLRQGRLAEAEMLLLGKD
                     GQLQALLPAARLHLARGDQDLARAT"
     gene            complement(962..1492)
                     /locus_tag="VHK28_08905"
     CDS             complement(962..1492)
                     /locus_tag="VHK28_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016690794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2142371.1"
                     /translation="MATTNPDQPHTKLATAGTAEKRGAAPMFRGWQWRAGNTLVSILA
                     RAGVGPIELLTTEGRVSGRPHTVPVVPIDHAGTRWLVAPYGPVAWVHNARARGVVTLR
                     YGRRRRVYAVREATPMEAAPILKRYVTVATRTRHRFAATVDAPVEAFIAEAADHPVFQ
                     LHPPSKGSTSVVDRKE"
     gene            1568..2167
                     /locus_tag="VHK28_08910"
     CDS             1568..2167
                     /locus_tag="VHK28_08910"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142372.1"
                     /translation="MGMRQAERRARTSQRLLDAAADVFTRLGFHAATVDDIADAAGYS
                     KGAVYGNFAGKDALFLALLDRHLDDQFTQVDLLLSAASDADLQSGLHDASTEHMTAGG
                     SFGLLMLEFWLYAARNAEARAALAARYERMRDRLAVVIADRDAARGIADSRAPDEVAA
                     LVLAVDAGLFLQHLIDPKAITPDLRANALVAVIDPTRHD"
BASE COUNT          328 a          807 c          747 g          320 t
ORIGIN      
        1 cggtggcacg agccaggtcc tgatcgccgc gagcgaggtg gaggcgggcg gcgggaagca
       61 gcgcctggag ctgcccgtcc ttgcccagca acagcatctc ggcctcggcc aggcggccct
      121 gccgcagcct gagctccgcc aaggcgatgg cggggtgcca gctgggcatg cccatgccgg
      181 cctcgaagtc ctcgatggcc cgggtcagca tggcctcggc ctcgccccat cgtccgagct
      241 cgcacagcgc ggtggcctgg acactgtcgc agtgactgct caggtacaac ggcgcccccg
      301 gagccttccc gatcagcccc tgacgacgaa gggcgtccgc ccaggaaccg gcccggtcga
      361 agtcggccgc gaagtagcac gcggtgaaga acgagcagac cgacttgccc gccgcgtcca
      421 ggttgtcagc cggcccgcag gccagggcca tggcctcgtc cagcatcgcc atgccctcgg
      481 ccacccgccc cacctgcacg caggcgaggc caccgtcggc cagagccttg gtctccaggt
      541 tcacatcccc gaactggcgg gcccgggcca gggccagctc ggcgcgcgcc agcagcacgg
      601 cggggtcatc gacgtcacag cccatcgccg ccaccgccac ccagccctgc tcgaggcatg
      661 gtggctcgtc ctcgatcaac cgggtggccc gcaggaacca ggcgcgcgcc gcggttctgt
      721 tcccgatggc attggcgtac aggtcgccca gccgagcaca cgtcagcgcc gcttgccggc
      781 gctcgcccgc ggcggtcaac gtgcggatcg cgccggacag gtgggcaacc gcggcatcga
      841 agtcgaacgc cctcagcgcc tcctcggcga gctccaggtg tcgcgccgcc ccctcctcga
      901 ccatcgccac cgaaccatgg tggcacgccc ttccggcgcc cgcaggggct gcctccggga
      961 ctcactcctt gcggtccacg accgacgtgc tccccttgga cggcgggtga agctggaaga
     1021 ccgggtggtc ggcggcttcg gcgatgaacg cctccacggg agcgtcgacc gtggcggcga
     1081 accggtgccg tgtccgggtg gcgacggtga cgtagcgctt gaggatgggc gccgcctcca
     1141 tcggtgtggc ttcgcggacg gcgtagaccc ggcgccgccg cccgtagcgc agggtgacga
     1201 caccgcgggc gcgggcgttg tggacccatg cgacagggcc atagggcgcg accaaccagc
     1261 gcgtaccggc atgatcgatc ggcacgacgg gcaccgtatg cggccgcccg ctcacccgac
     1321 cctccgtggt cagcagctcg atcgggccga ccccggcccg ggccaggatg ctgacgagcg
     1381 tgttgccggc gcgccactgc cagccacgga acatcggggc ggccccgcgc ttctcggctg
     1441 ttcccgccgt cgccagcttg gtgtgcggtt gatccggatt ggtggtcgcc atcgtcgcct
     1501 ccatcgcgta tagtttgtat accgctgagt attctatacc ggtcagtatt caaacaccag
     1561 aggggtcatg gggatgcgac aggcagagcg tcgagcgcga acgagtcagc ggctgctcga
     1621 cgccgcggcg gacgtgttca cccggctggg gttccatgcc gccaccgtcg acgacatcgc
     1681 cgacgccgcg ggatattcga agggtgcggt gtacggcaac ttcgccggca aggacgcgct
     1741 gttcctcgcg ctgctcgacc gccacctcga tgaccagttc acccaggtcg acctgctgtt
     1801 gtcagccgca tcggacgccg acctgcagtc aggtctgcac gacgcgtcga cggagcacat
     1861 gacagccggc ggctcgttcg gactcttgat gctggagttc tggctgtacg ccgcccggaa
     1921 tgccgaggcc agagcggccc tggccgcgcg ctacgagcgg atgcgcgacc ggttggccgt
     1981 ggttatcgcc gaccgcgatg cggcacgagg gatcgccgac tccagggccc ctgatgaggt
     2041 ggccgctctg gtcctcgcgg tcgacgccgg cctgttcctg cagcacctca tcgatcccaa
     2101 ggccatcaca ccggacctgc gcgccaacgc gctcgtcgca gtcatcgatc ccacccgcca
     2161 cgactgacag cggcttggcg gctcgggcac ccaacccgcc gt
//
LOCUS       DATACA010000439         1736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_702357, whole genome shotgun sequence.
ACCESSION   DATACA010000439 DATACA010000000
VERSION     DATACA010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702357"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHK28_08915"
     CDS             <1..228
                     /locus_tag="VHK28_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142373.1"
                     /translation="LGGREFLGWQLVQKMRMKPETARIPIIICTAAQREVQEQEGWLA
                     KNGVTVVLKPFDLEELERAVDRSLRLAEMNG"
     gene            complement(155..571)
                     /locus_tag="VHK28_08920"
     CDS             complement(155..571)
                     /locus_tag="VHK28_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142374.1"
                     /translation="MEQVADDSGVVRTLRVQGAYYLLSGVAPLVAYRAFIWVTGPKRD
                     DWLVKTFGLVVAGFGAALLRRPSPEVASLAATVAGAIGLAEAWYVLRGRIRAVYLADA
                     VVEGILAARLAAFSRSSRRAEGSDRPPAPAPRDRRA"
     gene            693..1274
                     /locus_tag="VHK28_08925"
     CDS             693..1274
                     /locus_tag="VHK28_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142375.1"
                     /translation="MTRTGSTGSAKTSRSTAVDDAASSPMEEAGQKAGKAASDVVDSA
                     KSTATERANMQKERVASGIQQVAEAIHRVSGELRVDQPAVASVADTAAQQAERIGSFL
                     RESDVNQLVRNTEDFARRQPLIFYGGAFAIGLLASRFLKAGASQQNGGRSWQATGPGR
                     TGSTYSGYQTGLAARHDGESTEPTWRPATSTGV"
     gene            1244..1669
                     /locus_tag="VHK28_08930"
     CDS             1244..1669
                     /locus_tag="VHK28_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX2142376.1"
                     /translation="MAAGDLDGGLTMQGQSERSLGDLFGDLGNQVSTLVRRELDLAKV
                     EVGDRVGRLGKDAAMVAVGGAVIHAAVLVLLAAAVLALIDAGLDGWLAALLVAAVVGL
                     LGGLLVWRGLAAMREDSNRPSATVETMRENIEWAKEQTK"
BASE COUNT          300 a          577 c          625 g          234 t
ORIGIN      
        1 ctgggcgggc gggagttcct cggctggcag ctggtgcaga agatgcgcat gaagccggag
       61 acggcccgca tcccgatcat catctgcacc gccgcccagc gggaggtcca ggagcaggag
      121 ggctggctgg ccaagaatgg cgtgaccgtg gtgctcaagc ccttcgatct cgaggagctg
      181 gagcgggcgg tcgatcgctc ccttcggctc gccgagatga acggctgaaa gccgcgagcc
      241 tcgctgccag gatcccctcc actaccgcgt ctgccaggta cacggcgcgg atgcgaccgc
      301 gcaggacgta ccatgcctcc gctagcccga tcgccccagc gacggtcgcc gccaggctcg
      361 ccacctcggg cgacggacgg cggagcaggg ccgcgccgaa gcccgccacc accaggccga
      421 acgtcttcac cagccagtcg tcgcgcttgg gtccagtgac ccagatgaag gcccgatagg
      481 cgacaagggg ggccacccca ctcagcaggt agtacgcacc ctgcacgcgc agcgtgcgca
      541 ccacgccaga atcatcagcc acctgctcca tcggtctcac gccatgcgtt ttgcgcaagg
      601 cgcgggccac ctattggcac tcgccatgcg ttcaggtcag ccgagccatt cgagttgtta
      661 ccgatgatgg cgagtacgaa agggggcact tcatgaccag gacgggatcg accgggtcgg
      721 ccaagaccag ccgttcgacg gcagtcgacg atgcggccag cagcccgatg gaggaggccg
      781 gccagaaggc gggcaaggcg gcgagcgacg tggtcgatag cgccaagtcg accgccaccg
      841 agcgcgccaa catgcagaag gagcgcgtcg cgagcggcat ccagcaggta gcggaggcca
      901 tccaccgcgt ctccggcgag ctgcgggttg accagccggc agtcgcctca gtcgccgaca
      961 cggccgccca gcaggcggag cgcatcggct cattcctgcg cgagtccgac gtcaaccagc
     1021 tggtgcgcaa taccgaggac ttcgcccggc ggcagccgct catcttctac ggcggcgcgt
     1081 tcgccattgg tctgctggcc tctcgcttcc tcaaggcggg cgcctcgcag cagaacggtg
     1141 gccgctcatg gcaggcgacc gggccgggac gcacgggcag cacctacagc ggctaccaga
     1201 ccggcctggc cgcgcggcat gacggggagt cgacggagcc gacatggcgg ccggcgacct
     1261 cgacgggggt ctgaccatgc aggggcagag cgaacggtcg ctcggcgacc tgttcggcga
     1321 ccttgggaac caggtcagca ccctggtccg ccgcgagctc gacctggcca aggtcgaggt
     1381 cggtgaccgg gtgggccggc tgggaaagga tgcggccatg gtggccgtcg gcggtgcggt
     1441 catccatgcc gcggtgctgg ttctgctggc cgcagcggtg ctggcgttga tcgacgccgg
     1501 ccttgacggc tggctggcgg cgctgctggt ggccgcggtg gttggcctgc tgggtgggct
     1561 gctggtgtgg cgcggcctgg ccgcgatgcg cgaggacagc aaccggccca gcgcaaccgt
     1621 cgagaccatg cgcgagaaca tcgagtgggc gaaggagcag acgaagtgat ggatcgccga
     1681 gacgacacgg atcgatacct cgaggagtcg gagatcgccc cggacgagag caccac
//
LOCUS       DATACA010000440         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1666263, whole genome shotgun sequence.
ACCESSION   DATACA010000440 DATACA010000000
VERSION     DATACA010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666263"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /gene="uvrA"
                     /locus_tag="VHK28_08935"
     CDS             <1..1804
                     /gene="uvrA"
                     /locus_tag="VHK28_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2142377.1"
                     /translation="RYRARNGNIHTFRTTFEGVVPNLQRRYRETGSEMIKAEIERYMT
                     TKPCPTCNGMRLKPESLSVTVGDRNVIETTQLSVTDALAWHQALPELLTERENHIARQ
                     VLKEIRSRLGFLVDVGLEYLTLDRASGTLSGGEAQRIRLATQIGSSLMGVLYILDEPS
                     IGLHQRDNDRLIATLVRLRDLGNTLIVVEHDEETIRNADWVIDIGPGAGEHGGTLIHS
                     GPLEDLLENSGSITADYLRGDRSVPVPRRRRKGSGEWLTVRGAGENNLRGIDVAFPLG
                     RFVAVTGVSGSGKSSLVSQILYPALAARIWGSRDRPGKHEAIDGIDHIDKVIEIDQSP
                     IGRTPRSNPATYTGLWAPLRELLAAVPEARLRGYKPGRFSFNVKGGRCEACEGAGIVQ
                     IEMHFLPDVYVTCEVCKGRRYNRETLEIHYKGKNVAEILEMTVDEALEFFSVVPSIRN
                     RLQTLHDVGMGYIHLGQPATTLSGGEAQRVKLATELSKRATGRTFYILDEPTTGLHFE
                     DVSRLLKVLGRLADAGNTVVVIEHNLDVIKSADWVIDLGPEGGDAGGALVAAGTPEQV
                     ARVAASYTGRYLRPALATARAVAERTRRRAAVPA"
     gene            1804..2370
                     /locus_tag="VHK28_08940"
     CDS             1804..2370
                     /locus_tag="VHK28_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2142378.1"
                     /translation="MAPTRADALALLEEWNENPSLRRHGLLVEAAVRGYATDDGVDAG
                     TVERWGIAGLLHDLDYERYPDPAQHGAIGADELARRGYPADVVEAVRSHNDALGIPRT
                     TRLDHLVYACDELSGFLVAVALMRSTKRLADVEPQQVRKRMKDKAFARAVPRDMLLAG
                     AEEIGLDFDEHVARMVVHLSQVAPELGL"
     gene            complement(2367..3974)
                     /locus_tag="VHK28_08945"
     CDS             complement(2367..3974)
                     /locus_tag="VHK28_08945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX2142379.1"
                     /translation="MAATLLASLDPEQRAAATLPDGPSLIIAPAGSGKTTTLIARLGV
                     LLERGVAAERIAVATFNRDAALDLSARIAARLAPVIPSAAAIEVRTLHALARRIVIDA
                     RGPVELVTDRMPLLRVVLRRAAAREPPEGDLPAIAELDTEVSAFKVERRPPAHRSLVE
                     EYRALLAARGALDFDDLVAEAGNLLESDPYVRLRWQSRFSHLCVDEFQDVDAGQLRLV
                     RLLAEPERNLFVVGDDDQTIYAWRLADVRRILTFESTYPGARRVQLATNYRCPAPVVG
                     ASRSLIGVNRERFAKRIDPAPNAPPAPATIAAFATTAPDWADRLAGLASLEGSAGRTI
                     CFLARTRSELAPVTLALVRVGVPHATSVPAPVQAEPVQALLRAARAQPAHLLPFDAML
                     RLRAAHRWRRTDADDTLGDDDHAALDALMGWTVGFRRLDAFLAAHDAALSRLESLRRD
                     EAPVELVTVHAAKGREWQTVVLLGWEEERFPNRRALVDAADPTRALEEERRLAYVALT
                     RATRRLILAFDPARPSRFLAEAGLAPC"
     gene            3964..>4368
                     /locus_tag="VHK28_08950"
     CDS             3964..>4368
                     /locus_tag="VHK28_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142380.1"
                     /translation="MAAIARGVSAVRVVVCMAGSMDDTRLLADDRPLISAVAAILWRA
                     SMFRRDRSRSHATATARARRVREAARRDEQLRAQGIDPLEYDRAAIRSGRASETRGYA
                     VARERNRPPIRRILLVGVMLLLTTVVVGSVLLW"
BASE COUNT          667 a         1533 c         1571 g          597 t
ORIGIN      
        1 ccgctaccga gcacgcaacg ggaacatcca caccttccgc accaccttcg agggcgtggt
       61 gccgaacctc cagcgccgct accgcgagac cggctccgag atgatcaagg ccgagatcga
      121 gcgctacatg accaccaagc cgtgccccac ctgcaacggc atgcggctga agcccgagtc
      181 gctgtcggtc acggtcggcg atcgcaacgt catcgagacc acgcagctgt cggtgaccga
      241 tgcgctggct tggcaccagg ccctgccgga gctcctgacc gagcgcgaga accacatcgc
      301 tcgccaggtg ctcaaggaga tccgatcccg gctcggcttc ctggttgacg tgggcctgga
      361 gtacctgacc ctggaccgcg cgtcggggac gctgtccggt ggcgaggcgc agcgcatccg
      421 gctggcgacc cagatcggct ccagcctgat gggtgtgctc tacatcctgg acgagccgtc
      481 cattggcctg caccagcggg acaacgaccg gctgatcgcc accctggtgc gcctgcggga
      541 cctgggcaac acgctgatcg tggtggagca cgacgaggag acgatccgca acgccgactg
      601 ggtgatcgac atcggtccgg gcgccgggga gcacgggggc acgctgattc attccgggcc
      661 gctggaggac ctgctggaga actcgggctc gatcaccgcc gactacctgc ggggagatcg
      721 gtccgtgccc gtcccgcgtc gccgtcgcaa ggggagtggc gagtggctga cggtgcgcgg
      781 cgcgggtgag aacaacctgc gcgggatcga cgtggccttc ccgctcggcc gcttcgtggc
      841 ggtcaccggg gtgagcggct ccgggaagtc gagcctggtc agccagatcc tgtatccggc
      901 gctggcggca cgcatctggg ggtcccggga ccgcccggga aagcacgagg ccatcgacgg
      961 catcgaccac atcgacaagg tgatcgagat cgaccagtcg cccatcggcc gaaccccgcg
     1021 ctccaacccg gccacctaca ccgggctgtg ggcaccgctg cgtgagctgc tggccgcggt
     1081 gccggaggcc cggctgcgcg gctacaagcc ggggcgcttc agcttcaacg tgaagggcgg
     1141 tcgttgcgag gcgtgcgagg gcgccggcat cgtgcagatc gagatgcact tcctgccgga
     1201 cgtgtacgtc acctgcgagg tctgcaaggg ccggcgctac aaccgcgaga cgctggagat
     1261 ccactacaag ggcaagaacg tcgccgagat cctggagatg acggtggacg aggcactcga
     1321 gttcttcagc gtggtgccgt ccatccgcaa caggctgcag accctgcacg acgtggggat
     1381 ggggtacatc cacctcggcc agccggcgac cacgctgtcc ggcggcgagg cgcagcgcgt
     1441 gaagctcgcc accgagctgt cgaagcgggc caccggccgg accttctaca tcctggacga
     1501 gccgaccacc ggcctgcact tcgaggacgt ctcgcggttg ctcaaggtgc tgggccggct
     1561 ggccgatgcc ggcaacaccg tcgtggtcat cgagcacaac ctggacgtca tcaagagcgc
     1621 ggactgggtg atcgatctcg ggcccgaggg cggagacgcg ggtggtgccc tggtcgccgc
     1681 cggcacaccc gagcaggtcg cgcgggtggc cgcctcgtac accggccgct acctgcgccc
     1741 ggcactggcc acggctcggg ccgtggccga gcgcacgcgg cggcgggccg ccgttcccgc
     1801 ctgatggccc caactcgcgc cgacgcgctg gccctgctgg aggaatggaa cgagaacccc
     1861 tcgctgcgcc gacacgggct gctggtggag gctgccgttc ggggatacgc gacggacgac
     1921 ggagtcgacg ccggcaccgt ggagcgctgg gggatcgccg gactgctcca cgacctggac
     1981 tacgagcggt accccgatcc agcacagcac ggcgccatcg gtgcggatga gctggcgcga
     2041 cgcggctacc ccgcggatgt ggtggaggcc gttcgctcgc acaacgacgc gctgggcata
     2101 ccgcgcacca ctcgcctgga ccacctggtc tacgcctgcg acgagctgtc cggattcctg
     2161 gtggcggtcg cgctcatgcg atccaccaag cggctggcgg acgtcgagcc gcagcaggtg
     2221 cgcaagcgga tgaaggacaa ggccttcgcc cgcgccgtgc cgcgcgacat gctgctggcc
     2281 ggggcggagg agatcgggct cgacttcgac gagcacgtcg cccgcatggt cgtccacctt
     2341 tcgcaggtgg cacctgagct tggcctctag cagggcgcca atccggcctc ggccaggaag
     2401 cgtgacggcc gcgccgggtc gaaagccagg atcaggcgcc gggtggcacg ggtgagcgcc
     2461 acgtaagcca gccgccgctc ctcctcgagc gctcgcgtcg ggtcagcggc atccaccagc
     2521 gcgcgacggt tcgggaaccg ctcttcctcc caacccaaca ggacgacggt ctgccactcg
     2581 cggcctttgg cggcgtgcac ggtgaccagc tccacgggtg cctcgtcccg gcgcagcgac
     2641 tcgagccgtg acagcgccgc atcgtgcgcg gccaggaagg catccagccg ccgaaagccg
     2701 acggtccagc ccatcaacgc gtccagggcc gcgtgatcgt cgtcgccgag cgtgtcgtcg
     2761 gcatcggtcc tgcgccagcg gtgggccgcg cgcaggcgaa gcatggcgtc gaagggaagc
     2821 aggtgcgccg gctgcgcacg ggcggcgcgc aggagcgcct ggaccggctc ggcctgtacg
     2881 ggagccggga cgctggtcgc gtgcggcacg ccaacccgca ccagcgcgag cgtcaccggc
     2941 gcgagctcgc tgcgggtccg cgccaggaag cagatcgtcc gtccggcact gccctcgagc
     3001 gatgccagcc cggccagccg atccgcccaa tcgggtgccg tggtggcgaa ggcggcgatg
     3061 gtggccgggg ccggcggcgc gttgggggcg ggatcgatcc gcttggcgaa gcgttcgcgg
     3121 ttgacgccga tcagcgagcg cgaggcgccg accaccggcg ccggacatcg gtagttggtg
     3181 gccagctgga cgcggcgcgc ccccggatag gtggactcga aggtcaggat cctccgcacg
     3241 tccgccagac gccaggcgta gatggtctgg tcatcgtcgc cgaccacgaa caggttgcgc
     3301 tccggctcgg ccagcaggcg caccaggcgc agctgcccgg cgtcgacgtc ctggaactcg
     3361 tccacgcaca ggtggctgaa gcgcgactgc cagcgcaggc gaacgtatgg gtcactttcc
     3421 aggagattgc ccgcctcggc caccaggtcg tcgaaatcca gcgctccacg ggctgccagc
     3481 agggcgcggt attcctccac cagggaccga tgcgccgggg gccgacgctc caccttgaag
     3541 gccgatacct cggtgtcgag ctcggcgatg gcggggaggt caccctcggg cggctcgcgc
     3601 gcggcggcgc ggcgcaggac gacgcgcagc agcggcatcc ggtcggtcac cagctcgacc
     3661 ggcccgcggg catcgatcac gatccgccgc gccagggcat gcagtgtccg gacctcgatg
     3721 gcggccgcgg acgggatcac cggggccagc cgcgcggcga tccgcgccga gagatcgagt
     3781 gccgcgtcgc ggttgaaggt ggccacggcg atccgttcgg ccgcgacgcc tcgctccagg
     3841 agcacgccca gccgggcgat caacgtggtg gtcttgccac tccccgccgg ggcgatgatc
     3901 agtgatggcc cgtcaggcag cgtggcggcc gctcgctgct ccgggtcgag cgaggcgagc
     3961 agcgtggccg ccatcgcccg tggcgtctcc gcggtccgcg tggtggtctg catggcgggc
     4021 agcatggacg acacccgctt actggccgat gatcgcccgc ttatcagcgc ggtcgccgct
     4081 atcctttggc gcgcctccat gttccgccga gaccgatccc gatcccacgc cacggcgact
     4141 gcccgtgccc gccgcgtccg tgaggcggcg cgccgcgacg agcagctgcg cgcacaggga
     4201 atagacccgc tcgagtacga ccgggctgcc atccgctccg gccgcgcctc cgagacgcgc
     4261 ggctacgccg tggcgcgcga gcgcaaccgg ccgccgatcc gccgcatcct gctcgtcggg
     4321 gtgatgctgc tgctgaccac ggtcgtggtc gggtcggtcc tcctctgg
//
LOCUS       DATACA010000441         2700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_221636, whole genome shotgun sequence.
ACCESSION   DATACA010000441 DATACA010000000
VERSION     DATACA010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221636"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VHK28_08955"
     CDS             <1..818
                     /locus_tag="VHK28_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX2142381.1"
                     /translation="AAAVALVFEPHPDEVLRPGTRVPRLAPLHTTLERILRDQGLDHA
                     IPLRFDDALRSLAAEEFLAAMAPSVQLRALIMSPESAFGRNRGGTVERMRQHGEEIGF
                     EVVTVEPVLVDGEVVSSTRQRTAIAAGDVAGALALGYPPRLEGTVVVGDRRGRLLGYP
                     TANLAFDYLPAMPARGIYVGWASVPERGVGPQHPALVSVGVRPTFHNTDDVLVEVHLL
                     DFDGDLYGAVLAVDLLARLRDELRFESAEELVDQMRRDEAAGRAFLAMRRRRE"
     gene            924..1193
                     /gene="rpsO"
                     /locus_tag="VHK28_08960"
     CDS             924..1193
                     /gene="rpsO"
                     /locus_tag="VHK28_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX2142382.1"
                     /translation="MPLAKDAKSAIVEDYATHEGDTGSPEVQIALLTERIKSLTEHLR
                     TYQNDNHSRRGLLKLVGQRRRLLAYLVRNDVDRYRSVIGRLGLRR"
     gene            1427..>2700
                     /locus_tag="VHK28_08965"
     CDS             1427..>2700
                     /locus_tag="VHK28_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX2142383.1"
                     /translation="MAIKVEADIGAQTFSLEAGKLAEQADGAVIVRYGDTVLLATAVS
                     AEPREGIDFFPMTVDYEERMYAAGKIPGGFIKRESRPSEAAILAMRLTDRPIRPLFPK
                     GYKQEVQIVLTVLSTDQENDPDILAINGASAALSLSSIPFLGPVGAVRVGRINGEFIT
                     NPTNSQLNDSELDLVVAGTRDALMMVEAGAKILPEAVMADAIAYGQAELQKSIDLQDK
                     LVANAGKPKKIPFIGPKADSVVKLGKALEDPNAEFVVLDLETTAMKPENGYIVDIAAL
                     KVRGGQVIDRFESLVNPGRSIIGHQVHGLSDQDVAGAPTAAEVLPRFAEWIGERPVIA
                     HNIGFDLPFVLRHMPNDVGWQPSAVYDTLELAYQLYPDAGGYKLSDLMRFVFGRDHGA
                     AHRAMPDAEATADLFINLTDGLVARLNAVRED"
BASE COUNT          430 a          930 c          938 g          402 t
ORIGIN      
        1 aggctgcggc ggtggcgctc gtgttcgagc cgcatcccga cgaggtgctg cgtcccggca
       61 cgcgggtccc gcggctggcg ccgctgcaca ccaccctgga gcgcatcctg cgcgaccagg
      121 gcctcgacca cgccatcccg ctgcgcttcg acgacgccct gcgctcgctg gccgccgagg
      181 agttcctggc ggcgatggcc ccatcggtcc agctccgggc gctgatcatg tcgccggaga
      241 gcgcgttcgg gcgcaaccgt ggcgggacgg tggagcgcat gcgccagcac ggtgaggaaa
      301 tcggcttcga ggtggtcacc gtggagccgg tgctggtcga cggcgaggtg gtgtcctcca
      361 cccggcagcg gacggcgatc gcggccggag acgtggccgg cgccctggca ctcggctacc
      421 cgccgcggct ggaggggacg gtggtggtcg gcgaccgccg cggccggctg ctcggctatc
      481 cgaccgccaa cctcgccttc gactaccttc cggccatgcc ggccaggggc atctacgtgg
      541 gctgggcgtc ggtgccggag cgtggtgttg gcccccagca cccggcgctg gtcagcgtgg
      601 gcgtgcgccc caccttccac aacaccgacg acgtcctggt agaggtccac ctgctggact
      661 tcgacgggga cctgtatggc gccgtgctcg cggtggacct gctggccagg ctgcgcgacg
      721 agcttcggtt cgagtccgcc gaggagctgg tggaccagat gcggcgtgac gaggcggccg
      781 gccgcgcgtt cctggccatg cgccgtcgga gggagtgact ccgcggtcgg catccacgcc
      841 aggggtgtaa cggccacttc ctggcggggg agggccgatt ctggtacgat ttcgcgcgac
      901 acgaaaggaa agtggaggaa tctgtgccgc tcgccaaaga cgcgaagagc gcgatcgtcg
      961 aggactacgc aacgcacgag ggggataccg gatctcccga ggtgcagatc gcgctgctca
     1021 ccgagcggat caagtcgctg acggaacacc tgcgcaccta ccagaacgac aaccactctc
     1081 ggcgcggcct gctcaagctg gtcggccagc ggcggcgcct gctcgcgtac ctggtccgca
     1141 acgacgtgga tcggtaccgt tcggtgatcg gtcgtctggg gctgcgccgc tgacgcgatt
     1201 cgagtaacga cccaggtgcc ggcaacgggc ctgggtcgcg atctgtccgg acatcgcatc
     1261 gggccgtccc gatcccggcg ccgaccggcg cccctatcca tcgacagcag ccgcgtccgc
     1321 gaggccgcct gcgcccctgc gttcgagccg agattcgctc gacagggcca gcgcgcgggc
     1381 cgcgcccgga gcgcaccttt gcatcatgag acaactggag gaatcagtgg ctatcaaggt
     1441 tgaggccgac atcggcgccc agaccttctc actcgaggct ggcaagctcg ccgagcaggc
     1501 cgacggggcg gtgatcgtcc gctacggcga caccgtcctg ctggcaaccg cggtctccgc
     1561 ggagccgagg gaggggatcg acttcttccc catgaccgtg gactacgagg agcgcatgta
     1621 cgctgccggc aagatcccgg gcggcttcat caagcgcgag tcgcgcccgt cggaggcggc
     1681 catcctggcc atgcgcctca ccgaccggcc cattcgcccg ctcttcccca agggctacaa
     1741 gcaggaagtg cagatcgtcc ttaccgtgct cagcacggac caggagaacg acccggacat
     1801 cctggccatc aacggcgcct cggcggcgct ctccctgagc agcatcccgt tcctgggccc
     1861 ggttggcgcg gtccgcgtcg ggcgcatcaa cggtgagttc atcaccaacc ccaccaacag
     1921 ccagctgaac gacagcgagc tggacctggt cgtggccggc acccgggacg cgctgatgat
     1981 ggtcgaggcc ggggccaaga tcctgcctga ggcggtcatg gcggatgcca tcgcctacgg
     2041 acaggcggag ctgcagaaga gcatcgacct gcaggacaag ctggtcgcca acgccggcaa
     2101 gccgaagaag atcccgttca tcggtccaaa ggccgactcg gtggtcaagc tgggaaaggc
     2161 cctggaggac ccgaacgccg agttcgtggt gctcgacctg gagaccaccg ccatgaagcc
     2221 ggagaacggc tacatcgtgg acatcgcggc gctcaaagtg cgcggcggcc aggtcatcga
     2281 ccgtttcgag tcgctggtca atcccgggcg ctcgatcatc ggccaccagg tgcacggcct
     2341 ctccgaccag gacgtggccg gcgccccgac cgcggccgag gtgctgccgc gcttcgccga
     2401 gtggatcggt gagcggccgg tgatcgccca caacatcggc ttcgacctgc cgttcgtgct
     2461 gcggcacatg cccaacgacg tcggctggca gccgtccgcg gtgtacgata cgctcgagct
     2521 ggcctaccag ctctaccccg atgcgggcgg ctacaagctc agcgacctga tgcgcttcgt
     2581 cttcgggcgc gaccacggcg ccgctcatcg ggccatgccg gatgcggagg ccacggccga
     2641 cctgttcatc aacctgaccg atgggctggt cgcccggctg aatgccgtgc gcgaggacat
//
LOCUS       DATACA010000442         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2469634, whole genome shotgun sequence.
ACCESSION   DATACA010000442 DATACA010000000
VERSION     DATACA010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469634"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /gene="dnaN"
                     /locus_tag="VHK28_08970"
     CDS             <1..1048
                     /gene="dnaN"
                     /locus_tag="VHK28_08970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX2142384.1"
                     /translation="TLPVLANVLLKTENAGLKLTATNLEIGITCWVPGKVEAEGDITV
                     PGRLLTDLVASLPNQRIDLQLSQKDRTLKVTCGGSRSSIKGIDADEFPVVAAIGEEPV
                     TSVDARALREGLGEVVFAAASDESRPILTGVLTRLSGSAMTLAAADNYRIAVRSVALA
                     RPVTPEQVIVVPARSYMELMRVLPDSETAIDITVTPNKSQVLFHVDEIDLVSRLIEGQ
                     FPNYEPVIPTSHSSRAVVDREAFLAGTRRAQIFARDSANIVKVEVGGDGEGISITAHA
                     ADVGDNADQLEANVEGQSTTIAFNARYLMDVLANLGSDEAALELSGPLAPGVIRGIGK
                     DDYVHVIMPVRTAS"
     gene            1091..>2083
                     /gene="recF"
                     /locus_tag="VHK28_08975"
     CDS             1091..>2083
                     /gene="recF"
                     /locus_tag="VHK28_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX2142385.1"
                     /translation="MRLTRLLLEDFRSYASAELHPDPGLTIVAGPNGAGKTNLLEAAH
                     VAITGRSHRAGTEAELVRHGQPFARVRLDLGSDDGRRDPARIELLLPGAEPPDGLRKR
                     LLVNGLPRRPSSVGEVARSVLFRPEEMLLVGSPSERRRFLDAILSQRDRRAARDLAEL
                     ARVLVQRNALLRAIRREEAGEEGLGFWDEQLAQVAGRVMAARLTLVRDLDARIPGLHD
                     AVAPADERGERVRLAYLDSLKEAWRARRAVEEDASGLADELAEAFRARIAAARQKEVW
                     NGVSLVGPQRDDLRVELAGRDVATHASRGQQRTIILAMKLAERELLAGGEAGMPI"
BASE COUNT          304 a          747 c          765 g          267 t
ORIGIN      
        1 caccctgccg gttctggcca acgtcctact caagacggag aacgcggggc tgaagctgac
       61 cgccaccaac ctggagatcg ggatcacctg ctgggtgccg ggcaaggtgg aggcggaggg
      121 cgacatcacc gtgccgggcc gcctgctgac cgacctggtt gcctcgctgc ccaaccagcg
      181 gatcgacctg cagctctcgc agaaggaccg caccctgaag gtcacctgcg gcggcagccg
      241 ctcgagcatc aagggcatcg acgccgacga gttcccggta gtcgccgcca tcggcgagga
      301 gccggtgacc agcgtcgacg ctcgcgccct gcgcgagggg ctgggcgagg tcgtcttcgc
      361 ggccgccagc gacgagagcc gcccgatcct gaccggcgtc ctgacccgcc tgtccggcag
      421 cgccatgacc ctggccgcgg ccgacaacta ccgcatcgcg gtgcgcagcg tggcgctggc
      481 ccggccggtg acaccggagc aggtgatcgt ggtcccggca cgctcctaca tggagctcat
      541 gcgggtgctg cccgacagcg agacggccat cgacatcacc gtcacgccca acaagagcca
      601 ggtgctgttc cacgtcgacg agatcgacct ggtcagccgg ctgatcgagg ggcagttccc
      661 gaattacgag ccggtgatcc cgaccagcca ctcgagccgc gcggtggtcg accgcgaggc
      721 cttcctggcc ggcacgcggc gggcccagat cttcgcgcgc gacagcgcga acatcgtcaa
      781 ggtcgaggtc ggcggcgacg gtgagggcat ctccatcacc gcccacgccg cggacgtcgg
      841 cgacaacgcg gaccagctgg aggccaacgt ggaggggcag tcgaccacca tcgccttcaa
      901 cgcgcggtac ctgatggacg tgctggccaa cctcggctcc gacgaggcgg cgctggaact
      961 ctccggaccg ctggcgcccg gcgtcatccg cggcatcggc aaggacgact acgtgcacgt
     1021 catcatgccg gtccgcaccg cgtcctgacc ggcctggacc gatgacggcg ccggcgaagg
     1081 ggggcgcccg atgcgcctga cgcggctgct gctcgaggac ttccgcagct acgcctccgc
     1141 cgagctgcac cccgatccgg ggctcaccat cgtggccggc cccaacggcg cgggcaagac
     1201 caacctgctg gaggcggcgc acgtggcgat caccgggcgg tcgcaccggg ccggcacgga
     1261 ggccgagctg gtgcgccacg gccagccctt cgcccgcgtc cggcttgacc tgggcagcga
     1321 cgacgggcgc cgggacccgg cgcgcatcga gctgctgctg cccggggcgg agccgccgga
     1381 cggcctgcgc aagcggttgc tggtcaacgg gcttccgcgg cgcccgtcca gcgtcggcga
     1441 agtggcgcgc tcggtcctct tccgtccgga ggagatgctg ctggtcggct cgccctccga
     1501 acggcgccgc ttcctggacg ccatcctctc gcagcgcgac cgccgggcgg cccgcgacct
     1561 ggccgagctg gcccgcgtcc tggtccagcg caacgcgctg ctgcgcgcca tccgtcgcga
     1621 ggaggccggg gaggagggcc tcggcttctg ggacgagcag ctggcgcagg tcgccggccg
     1681 ggtcatggcc gcccggctga cgctggttcg cgacttggat gcccgcatcc ccggcctgca
     1741 cgatgcggtg gcgcccgccg acgagcgcgg ggagcgggtg cggctggcgt acctggacag
     1801 cctgaaagag gcgtggcggg cacggcgagc cgtggaggag gacgcctccg gcctggcgga
     1861 cgagctggcg gaggccttcc gtgcgcgcat cgcggctgcg cgacagaagg aagtgtggaa
     1921 cggcgtcagc ctggttggcc cgcagcggga cgacctgcgc gtggagctgg ctggtcgcga
     1981 tgtcgcgacc cacgcgtcgc gcggtcagca gcggaccatc atcctggcca tgaagctggc
     2041 cgagcgcgag ctgctggccg gcggcgaggc cggcatgccg atc
//
LOCUS       DATACA010000443         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_622334, whole genome shotgun sequence.
ACCESSION   DATACA010000443 DATACA010000000
VERSION     DATACA010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622334"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VHK28_08980"
     CDS             complement(<1..433)
                     /locus_tag="VHK28_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142386.1"
                     /translation="MAATKVLVVDDDLNIQRVLVFTLKQEGFEVLVASDGQAGVQMAR
                     DAQPDLVLMDVAMPKLDGYAATQQIRAAETDGRRVPIIMLTTEADVGERVKGLRAGAD
                     DDIVKPFHPLELMARIKALLARGGALTQATSPDAATLGQLCV"
     gene            complement(490..1410)
                     /locus_tag="VHK28_08985"
     CDS             complement(490..1410)
                     /locus_tag="VHK28_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2142387.1"
                     /translation="MGTVRRRSMAAWLGRIVAAGAAGYLGFLGVEGSRRVVRPGRKLL
                     EAEDGGPANPADLELPYEDVRFTTDDGYTLAGWLIPSERRTRSAVILLHGFSWHRLPW
                     LTDFVPWIRPRYNVLMFDFRGHGASDDAPITLGTSEQRDVAAAVRFLESRGFGPIALL
                     GISMGGAVAIMSAPDLPVAAVVADAAYAEVAHPVANKMRQGHYPFPRLGSRLIVAAAS
                     LRARVRLREPIARVSEISPRGLLLIAPRDDQVVSWTQSLKMYEAAQEPKELFVVDGCG
                     HSEAHAVAGPEYERRVLAFLGRYLDGGTPV"
     gene            complement(1424..2329)
                     /locus_tag="VHK28_08990"
     CDS             complement(1424..2329)
                     /locus_tag="VHK28_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2142388.1"
                     /translation="MDNPVTLAILIVAAVFLLFLIYSTIHIVRQYERLVIFTLGRVTN
                     GNAGPGLVLLVPIVQQPVKVDLREFYIELPPQTCITKDNAPISIDFLVYSKVFDPEQS
                     VTAVTSFSGASQALAATTLRAVIGDIPLDDVLAKREEINNILRTKLDEVTHRWGVKIT
                     SVEIREIVPPRDIQDAMNRQMSAERNRRANITESEGTRQATINVAEGDKQSNILRAEG
                     ERQSQILRAEGFSNALRTIFEAARGVDDKTMALQYLEALKALGAGESTKWVIPMELSE
                     LTRPITGAMRSAQQAGGGPETTAGS"
     gene            complement(2408..>3314)
                     /locus_tag="VHK28_08995"
     CDS             complement(2408..>3314)
                     /locus_tag="VHK28_08995"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2142389.1"
                     /translation="AGTRVVLDPLLGCTVRGITPPCEWCAAGQPGLCLRTAEGGFAPG
                     PMIGFCSDLPGGWADEMVAHRSQLHVVPEGLTDETAVMVEPLAVALHGVLAGPPADDA
                     RVLVIGGGTLGLCTLAALRLAAPAADVFVLARHPAQRRLAERLGATAVVTAAVQAAVE
                     RAGARRYRSLVGEDVLTGGFDQVYDAVGSTRSLDAAVRVTAPRGRLVLIGGPGHLAGL
                     DWTLVWTRELRVAGTYVYGREAGVDGEPHTMDLAMRLLVTRPDLSLGELVTHRFRLDE
                     WRSAMAAALDRRRSGAVKIAFAPAG"
BASE COUNT          493 a         1200 c         1146 g          475 t
ORIGIN      
        1 agacgcacag ctgaccgagg gtggcggcat ccggcgaggt ggcctgcgtc agcgcaccac
       61 cccgcgcgag cagggccttg atgcgtgcca tcagctccag tgggtggaac ggcttgacga
      121 tatcgtcgtc cgcgccggca cgcaggccct tcacccgctc gccgacgtcc gcctcggtgg
      181 tgagcatgat gatcgggacc cgtcgtccgt cggtctcggc cgcgcgaatc tgctgggtcg
      241 ccgcatagcc gtcgagcttg ggcatggcga cgtccatcag gaccaggtcc ggctgggcat
      301 cacgggccat ctgcacgccc gcctgtccat cggaggcaac gaggacctcg aagccctcct
      361 gcttgagggt gaagacgagg acgcgctgga tgttcaggtc gtcatcgacg acaaggacct
      421 tcgtggcagc catcaaggac ccctggtgga tgtggatggg cgtcgcaggc gcccgtgcgc
      481 cggccgagat tatacgggcg tgccaccgtc caggtagcgt cccaggaagg cgagcacgcg
      541 ccgctcgtac tccgggccgg cgaccgcgtg cgcctcgctg tgcccgcagc cgtccaccac
      601 gaacagctcc ttcggctcct gcgcggcctc gtacatcttc agcgactgcg tccaggagac
      661 cacctggtcg tcgcgcggcg cgataagcag cagcccgcgg ggcgagatct cggatacgcg
      721 ggcgatcggc tcccgcaggc gcacccgcgc ccgcagcgag gcggcggcca cgatcagccg
      781 cgacccgagg cgcgggaacg ggtagtggcc ctggcgcatc ttgttggcaa cggggtgcgc
      841 gacctcggcg taggcggcgt ccgcgaccac ggccgccacc gggagatccg gcgcggacat
      901 gatggccacc gcgccgccca tgctgattcc cagcagggcg atcggcccga agccgcgact
      961 ctccaggaag cgcacggcgg cggcaacgtc acgctgctcg ctggtcccga gcgtgatggg
     1021 cgcgtcgtct gaggcgccat ggccgcggaa gtcgaacatc agcacgttgt atcgcggccg
     1081 gatccacggc acgaagtcgg tcaaccaggg cagccggtgc cagctgaagc cgtggagcag
     1141 gatcaccgcc gagcgcgtcc ttcgctcgga ggggatcagc cagccggcga gggtgtagcc
     1201 gtcatcggtc gtgaagcgca cgtcctcgta gggcagctcc aggtcagccg ggttggccgg
     1261 cccgccgtcc tcggcctcca gcagcttgcg acccgggcgc accacccggc gcgagccctc
     1321 cacccccagg aagccgaggt acccggccgc cccggccgcc acgatgcgac ccagccaggc
     1381 cgccatgctg cggcggcgca ccgtccccat ccgccccgcc acctcagctg cccgccgtgg
     1441 tctccggacc gccgccggcc tgctgcgccg atcgcatggc gccggtgatc ggccgcgtca
     1501 gctcggatag ctccatgggt atcacccact tggttgactc gccggcgccg agcgccttga
     1561 gcgcctccag gtactggagc gccatcgtct tgtcgtccac gccgcgcgcc gcctcgaaga
     1621 tggtgcgcag cgcgttgctg aagccctcgg cgcgcaggat ctgggactgc cgctcgccct
     1681 cggcgcgcag gatgttcgac tgcttgtcgc cctcggcgac gttgatggtg gcctgccggg
     1741 ttccctccga ctcggtgatg ttcgcgcggc ggttgcgctc ggcgctcatc tggcgattca
     1801 tggcgtcctg gatgtcgcgc ggcggcacga tctcgcggat ctcgacgctg gtgatcttga
     1861 cgccccagcg gtgggtgacc tcgtcgagct tggtgcgcag gatgttgttg atttcctcgc
     1921 gcttggccag cacgtcgtcc agcggaatgt cgccgataac ggcacgcagg gtggtcgccg
     1981 ccagcgcctg cgaggcgccg gagaagctgg tgacggcggt gaccgactgc tcggggtcga
     2041 agaccttgga gtacaccagg aagtcgatgc tgatgggcgc gttgtccttg gtgatgcagg
     2101 tctgcggcgg cagctcgatg tagaactcgc gcaggtccac cttcaccggc tgctgaacaa
     2161 tgggcaccag cagcaccagg ccgggcccgg cgttgccgtt cgtcacgcgc ccaagggtga
     2221 agatcaccag tcgctcgtac tgccgaacga tgtggatggt gctgtagatc aggaacagca
     2281 ggaagacggc ggcgacgatc aggatcgcca gcgtgaccgg attgtccatg tcgctcgggg
     2341 cctccatcgg gcggtggacc ctgagcatag cgctccatgc gcgccccttc ccgaacggcc
     2401 tccgtgctca gccggccggg gcgaacgcga tcttgactgc cccgctccgt cggcggtcga
     2461 gcgccgccgc catggccgat cgccactcgt caagccggaa ccgatgcgtc accagctcac
     2521 cgagcgacag gtccggacgc gtgaccagca ggcgcatggc gaggtccatg gtgtggggct
     2581 cgccatccac gccggcctcc cgcccgtaga cgtaggtccc ggccacgcgc agctcgcgcg
     2641 tccagaccag cgtccagtcc aggccggcca aatggcccgg cccgccgatt aggacgaggc
     2701 gtcctcgtgg cgccgtcacc cggacagcag cgtccagaga ccgcgtgctg ccgacggcgt
     2761 cgtacacctg gtcgaaacca ccggtcagca cgtcctcccc caccagggac cgatagcgcc
     2821 gcgcgccggc tcgctccacc gcggcctgca ccgcggcagt caccaccgcg gtggcgccca
     2881 ggcgttcggc caggcggcgc tgggcggggt gccgcgccag cacgaacaca tccgctgccg
     2941 gagctgccag ccgcagcgca gcgagcgtgc agagcccgag cgtccctccg ccgatgacca
     3001 gcacccgcgc atcgtcggcc ggcggcccgg ccagcacgcc gtgcagggcc accgccagcg
     3061 gctcgaccat gaccgccgtc tcgtcggtta gcccctccgg gacgacgtgg agctgggacc
     3121 gatgcgcgac catctcatcg gcccagccgc cggggaggtc gctgcagaag ccaatcatcg
     3181 gcccgggcgc gaagccgcct tccgccgtgc gcaggcagag cccgggctgc ccggctgcac
     3241 accactcgca cggcggcgtg atcccgcgca cggtgcatcc cagcaggggg tcgagcacga
     3301 cccgggtgcc ggcc
//
LOCUS       DATACA010000444         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2310007, whole genome shotgun sequence.
ACCESSION   DATACA010000444 DATACA010000000
VERSION     DATACA010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310007"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VHK28_09000"
     CDS             complement(<1..720)
                     /locus_tag="VHK28_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2142390.1"
                     /translation="MRLTVLGRSPARPNPNEACAGYLVEGGGSRVLMDVGPGVVAQLL
                     RRNTPDELDAVVISHMHTDHCLDLVTLRYSYPWIDVAKNRLLVIVPPGATSQLSELAL
                     GAGYSNFFDKSFTFREHDGKSPIEIGNLRFEPDETQHFIPTWGFRVTARGTDEDSDRV
                     LAYSADAGPCEALPRIADDADLFLCEAALRSLDEDDSEPGRRGHLLPSEAATVAAHSG
                     ARGLLLTHIPLPDGGEWAMEQA"
     gene            839..1585
                     /locus_tag="VHK28_09005"
     CDS             839..1585
                     /locus_tag="VHK28_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142391.1"
                     /translation="MAESKHRRKGRSRLPAVIRVGDRTAEVSSEFRRHDPGPGVVTPT
                     EREIRHAIASIPDDLDWAWARGRIAPVFERPGAGSPNGDPILHGVSELGVAFAFAIEV
                     GPLFTPVTASMAERWEASLEQIEAAAFDHLGAAVAPVTRQQVQHAVHRGHLIRFLPEP
                     AGWASSVILAGVDEVTRIFGPQDQIFTVPSRNGLMSFSAATPIGTVFDVTLGMEEMDL
                     HPLELDPFVLEDGRLSWTGVLDDEDLSADR"
     gene            complement(1569..>2687)
                     /locus_tag="VHK28_09010"
     CDS             complement(1569..>2687)
                     /locus_tag="VHK28_09010"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX2142392.1"
                     /translation="TYLDGEQGILRYRGYPIEQLAENSSFLEVAYLLIYGELPSRQQL
                     DRFQAEIVHHTLIREEMRHFFDSFPQDAHPMAVLAAGTVGMSTFYPDSQEIFDPAAVE
                     ITTIRLIAKLPTLAAWSFKKAIGQPYVYPRNDLGYAANLLHMMFAVPSEPYEVQPEVA
                     EALDLLLILHADHEQNCSTSTVRMVGSAHTNLYGSISAGISALWGPLHGGANEAVINT
                     LERIVADGGDVQRWVNRAKDPDSSFKLPGFGHRVYKNFDPRMRIIKGAAERVLAATGA
                     DDALFEVAQKLEDVALNDEYFVQRKLYPNVDFYSGLIYRAIGFPKNMYTNCFAIGRLP
                     GWIAQWREMITSKETKIGRPRQVYTGAAQRDYVPIDQR"
BASE COUNT          413 a          868 c          961 g          445 t
ORIGIN      
        1 cgcctgctcc atcgcccact ccccgccgtc cggcagcggg atgtgggtca gcagcagccc
       61 gcgcgcgccg gagtgggcgg ccacggtggc cgcctcggac ggaagcaggt ggccgcgacg
      121 tcccggctcc gagtcgtcct cgtccaggct gcgcagcgcc gcctcgcaca ggaaaagatc
      181 ggcgtcgtcc gcgatccggg gcagcgcttc gcatgggccg gcatcggccg agtaggccag
      241 cacccggtcg gagtcctcat cggtcccgcg cgcggttacc cggaagcccc aggtcgggat
      301 gaagtgctgg gtctcgtcgg gctcgaagcg caggttcccg atctcgatgg ggctcttccc
      361 gtcgtgctcg cggaaggtga agctcttgtc gaagaagttg ctgtagccgg ctcccagcgc
      421 cagctccgac agctgggagg tggcccccgg cggcacgatg accagcagcc gattcttggc
      481 gacgtcgatc cacgggtagc tgtagcgcag agtgaccagg tcgaggcagt ggtcggtgtg
      541 catgtggctg atgaccactg cgtcgagctc gtccggcgtg ttgcgtcgca gcagctgggc
      601 gaccacgccc ggacccacgt ccatcagcac gcgcgaaccg ccgccctcca ccaggtaacc
      661 ggcgcaggcc tcgttggggt tcggtcgggc agggctgcgg cccaggacgg tcagtcgcat
      721 ggcccgcgta gtgttgccca acacgtcaag cccgcgtcag tcacaaatgc gaccgcgccc
      781 ggcagggtga taagcgcctg gtaagccgac ggtaagggcc gatctggatg ctgtcgacat
      841 ggcagagagc aagcaccggc gcaagggtcg ctcccgcctg ccggcggtga tacgagtggg
      901 cgaccggacg gcggaagtca gcagcgagtt tcgaaggcat gaccccggac ccggtgtcgt
      961 cacgcccacc gagcgcgaga ttcgccacgc gatagcctcg atcccggacg acctggactg
     1021 ggcctgggca agggggcgga tcgcccccgt cttcgagcgg ccgggcgccg gcagcccaaa
     1081 cggcgacccg atcctgcacg gggtgagcga gctgggagtg gcgttcgcat tcgccatcga
     1141 ggtgggaccg ctgttcacgc cggtcaccgc ttcgatggcc gagcgctggg aggcgagcct
     1201 ggagcagatc gaggccgccg ccttcgacca cctcggcgcc gccgtggctc ccgtcacgcg
     1261 ccagcaggtg cagcacgcgg tgcaccgtgg ccacctgatc agattcctgc cggagccggc
     1321 aggctgggcg tcctcggtga tcctggcggg ggtggacgag gtgacgcgca tcttcggccc
     1381 acaggaccag atcttcacgg tgcccagccg caacggactc atgtcgttca gcgctgccac
     1441 cccgattggc acggtctttg acgtgacgct cggcatggag gagatggatc ttcaccctct
     1501 cgagctggac ccgttcgtcc tcgaggatgg acgcctgtcc tggacgggcg tcctcgacga
     1561 cgaggacctc agcgctgatc gatagggacg tagtcgcgct gtgcggcacc ggtgtagacc
     1621 tggcgcgggc ggccgatctt ggtctccttg gaggtgatca tctcgcgcca ctgcgcgatc
     1681 cagcccggca ggcgcccgat ggcgaagcag ttggtgtaca tgttcttggg gaagccgatg
     1741 gctctgtaga tcagcccgga gtagaagtcc acgttcgggt acagcttgcg ctggacgaag
     1801 tactcgtcgt tgagcgccac gtcctcgagc ttctgggcca cctcgaacag cgcgtcgtcc
     1861 gcgccggtgg cggccagcac ccgctcggct gcccccttga tgatgcgcat ccgcgggtcg
     1921 aagttcttgt agacgcggtg accgaagccc ggcagcttga agctgctgtc cgggtccttg
     1981 gcgcgattca cccagcgctg gacgtcgccg ccgtcggcca cgatgcgctc cagggtgttg
     2041 atcaccgcct cgttggcccc tccgtgcagc ggcccccaca gggcgctgat gccggcgctg
     2101 atcgagccgt acaggttggt gtgggcgctg cccaccatgc ggacggttga cgtggagcag
     2161 ttctgctcgt ggtcggcgtg caggatgagc agcaggtcga gcgcctcggc aacctccggc
     2221 tggacctcgt acggctcgga cggcacggcg aacatcatgt gcagcaggtt ggcggcgtag
     2281 cccaggtcgt tgcgcgggta gacgtatggc tgaccgatgg ccttcttgaa cgaccaggcc
     2341 gccagggtgg ggagcttggc gatgaggcga atggtggtga tctcgacggc cgccgggtcg
     2401 aagatctcct gcgagtcggg atagaacgtg ctcatgccca cggtgcccgc cgccagcacg
     2461 gccatggggt gcgcgtcctg cgggaaggag tcgaagaagt ggcgcatctc ctcgcggatc
     2521 agggtgtggt ggacgatctc cgcctggaag cggtcgagct gctggcgact ggggagctcg
     2581 ccgtagatca gcaggtaggc gacctccagg aagctgctgt tctccgccag ttgctcgatg
     2641 gggtagccgc gatagcgcag gatgccctgc tcgccgtcca ggtaggt
//
LOCUS       DATACA010000445         5155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2469854, whole genome shotgun sequence.
ACCESSION   DATACA010000445 DATACA010000000
VERSION     DATACA010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2469854"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(666..3500)
                     /locus_tag="VHK28_09015"
     CDS             complement(666..3500)
                     /locus_tag="VHK28_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009473020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2142393.1"
                     /translation="MPGTSNRGMRVTGGVGDGETSEDSPRRPGARAERRGVQLLGRQA
                     ECEQLDRLLAAVRAGESRALVVRGEAGVGKSALLEHLTRAASDCRVLGAAGVQSEMEL
                     AFAAVHQLCRPLLDRLDGLPGPQRDALYTAFGLRGGPPPDLFLVGLAVLSLLAAAAED
                     RPLVCVIDDAQWLDRASAQVLAFVGRRLFAESVACVFAVRDPSEEDQLSGLPVMGVAG
                     LGDDDARALLKTVIQGPLDEQVREQIIAEARGNPLALLELPHELPSIRLARGFAPPAA
                     RTLSARIEKSFQLRLERLPAPTHQLLLRAAAEPLGDPVLLWRAADTLGDGVGIAAVDG
                     AEGLIDVGDRVRFRHPLVRSAVYLAATPEQRRRAHQVLAEVTEIDADPDRHAWHRAHS
                     AAQPDEQTAAELEHSAARAQARGGLAAAAAFLERAVALTPDSARRAQRALAAARATHH
                     AGASGTALKLLSIALAGPLDDLRHGQAELVRAQIAYSTDRGRDSFALLLEAARRLQPH
                     DLPLARETYLEAINAAQFAGPLAVDGELRAAEAARSVPGATGDPRPADRLLDGLTTLI
                     IDGYGAGVPSLQPALSAFTEPDLSPEQGLRWLWLAGTTAATLWDYETWDVLAARHIRL
                     VRETGDATMLPFALTALVAIKMIAGELGTAASLMEEVKTASEVIGVAYPVHGALLTAA
                     GQGRQADHDELMSTVVAEASHRGEGSALIVSGWTRALLCNSRGRYKDALVAASQTTDH
                     PQREISAALGWTLVEEVEAAARSGVPERAADAFRRLRERTGPSGTGWGLGVQARSQAL
                     ISSGADAEDHYREAIERLRLTRVRGELARAHLLYGEWLRRERRRSQAREQLRTAHEMF
                     TAMGMEAFADRAARELRATGETARKRTVETTGQLTPQEAQIARLAREGLTNKEIASRL
                     YLSTRTVEYHLHKVFAKLGITSRNQLGSIP"
     gene            4204..5100
                     /locus_tag="VHK28_09020"
     CDS             4204..5100
                     /locus_tag="VHK28_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2142394.1"
                     /translation="MPTDDRVAVHDLEDNHNATIVLTGATAGIGRATALVLAPAAGHL
                     ILHGLERQDDIADLLRALRAAMRPDTRLTYVRADYSDLADVEQLARHIRSATDGIDIL
                     INNAARPGPPTRTVSAAGHEITFQTNYLAPVLLTSRIIDLIGSGARQGRIVNVASATH
                     LSATLLLDDLDLAHHPYSASTAYAHSKLALVTYSCWLAAHRPSRSLDAVSMHPGVIST
                     PLLHAMFSVAGDRPEHAAANIHYVASRRDDNGTYYDERNPTPPNPQATDRAQQDRLHE
                     ATVRALHLSPRAHVTSERQLPS"
BASE COUNT          799 a         1912 c         1696 g          748 t
ORIGIN      
        1 tcccagtccc cgcaccgact ccgctccagc acgacggccg gtcggtgatc gatcaacata
       61 agcgggcaga tcaatcgtgc gcgccgttcg tcagggcggc cccggcgctt gcgcagcggc
      121 ctcccggtgc gcagccggcg ggtgagaagg tctgacgtag atgcgcggcg atctgctccg
      181 ggctccactc cagctccagc ttgtcctgca cctcggcgcg cagctgcaga tcgtcgacca
      241 ggcgcgagcg acgcggccgc tgcgctcgct gccgggcccg ggcacgagcg agatcaccgt
      301 cgtaaccgcc tcggtcgtgc ggcagcgtgt tgcggtgcag ctcgcggctg gccggggatg
      361 ctgccccccg atcgcggcgg cgatcgcact cacgcccaga ccctggcggt gcaccgtggc
      421 gatccgctgg cgctccagca gcgacaagaa ccggtccgat cgcggccgtt ccggcagcgt
      481 cgctgtggac gaaggccggc gttctccgcg cgccaccctc gtccggatgg agggcgatgc
      541 gagtgcgtac ggcaggcatc acaggtcgtg gcccgtgtgc attcgcattc gcggctccac
      601 aacaagacct cacgctggta cgcggaccag cagcgccgag tcggttcctc caccgcatcc
      661 acaggtcacg gaatgcttcc cagctggttc cgcgaggtta ttcccagctt ggcgaagacc
      721 ttgtgcaggt ggtactcgac cgtgcgggtg ctgaggtaca agcggctggc gatctccttg
      781 ttcgtcagcc cctcgcgggc aagcctggcg atctgcgctt cctgcggcgt gagctggccg
      841 gtggtctcca cggtccgttt gcgggcggtc tcaccggtgg cccgcagctc gcgggccgcc
      901 cggtcagcga acgcctccat acccatggcc gtgaacatct cgtgggcggt gcgcaactgc
      961 tcacgagcct gggaccggcg ccgctcacgc cgcagccatt cgccgtagag gaggtgggcg
     1021 cgggccagtt cgccgcggac gcgagtcagg cggagccgct cgatcgcctc acggtaatgg
     1081 tcctcggcgt ccgcgccgct gctgatcagg gcctgcgaac gggcctggac gcccagcccc
     1141 cagcccgtac cgctggggcc ggttcgctcg cggagccgcc ggaaggcgtc agcggctcgc
     1201 tcgggcacgc cactgcgggc ggccgcttcc acttcctcca ccagtgtcca gcccagcgcg
     1261 gcgctgatct cccgctgcgg gtggtcggtc gtctggctcg cggcgaccag cgcgtccttg
     1321 tagcggccac ggctgttgca cagcagcgct ctggtccatc cgctgacgat caacgcgcta
     1381 ccctcgcccc ggtgggacgc ctccgcgacg accgtgctca tcagctcgtc atggtcggct
     1441 tggcggcctt gcccggccgc ggtcagcagt gccccatgga cggggtaggc gaccccgatc
     1501 acctcggagg ccgtcttgac ctcctccatc agtgaagcgg ccgtacccag ctcgcctgcg
     1561 atcatcttga tggcgaccaa cgcggtcagc gcgaagggga gcatcgtggc gtccccggtt
     1621 tccctgacga gccggatgtg ccgggctgcg aggacgtccc aagtctcgta gtcccacagg
     1681 gtcgcggccg ttgtgcccgc gagccacagc cagcgcagtc cctgctccgg ggacaggtcc
     1741 ggttctgtga acgcactcag tgccggctgc aggcttggta ccccagcacc gtatccgtca
     1801 atgatcaatg ttgtcaggcc gtcgagcagc cgatcggcgg gacggggatc ccccgtcgcc
     1861 cccggcaccg accgggcggc ctcggccgct ctgagttcgc cgtcgacggc caacggaccg
     1921 gcgaactgtg ccgcgttgat cgcttcgagg taggtctctc gggcaagagg caggtcatgc
     1981 ggctgaagcc ggcgggcggc ctcgagcagg agcgcgaaag agtccctgcc acggtccgtg
     2041 gagtaagcga tctgggcgcg caccaattcg gcctgaccat gccgtagatc atcgagcggg
     2101 cccgccaggg cgatggacag caacttgagt gcggtaccgg acgccccggc gtggtgagtg
     2161 gcgcgagcag cggccagtgc gcgctgggct cggcgcgcgg agtccggggt aagtgcgacc
     2221 gcccgctcca gaaaggcggc cgctgcggcc aggccgccgc gagcctgggc gcgtgcggcc
     2281 gagtgttcga gttccgcggc cgtctgctcg tcgggctgcg cggcgctgtg cgcgcggtgc
     2341 caggcgtgcc gatccggatc ggcgtcgatt tcggtcacct cggcgaggac ctgatgagcg
     2401 cgcctgcgct gctccggcgt cgcggccaga tagaccgccg aacgcaccaa cggatgccgg
     2461 aaacgcaccc ggtccccgac gtcgatcagc ccttcggcgc cgtccaccgc cgcgatgccg
     2521 acaccgtccc cgagcgtgtc ggcggcacgc cacagcaaca ccgggtcgcc gagcggctcg
     2581 gcggccgccc gcagcaacag ctggtgggtg ggggcgggca ggcgttccag ccgcagctgg
     2641 aagctctttt cgatgcgggc cgagagcgtg cgcgctgctg gcggagcgaa cccgcgggcg
     2701 agcctgatgg aaggcagctc gtgcggcagt tcgagcaacg ccagtgggtt gccccgagcc
     2761 tcggcgatga tctgttcgcg tacctgttcg tccagcggac cctggatcac cgtcttcagc
     2821 agcgcccgcg cgtcgtcgtc gcccagtccc gcgaccccca tgacgggcag acccgaaagc
     2881 tggtcctcct cgctcgggtc acggacggcg aacacgcagg ccaccgactc ggcgaacagg
     2941 cggcgcccca cgaaggccag cacctgcgcg gaggcacgat ccagccactg ggcatcgtcg
     3001 atcacgcaca ccagcggacg gtcctcggcc gccgccgcca gcaggctcag cacggcgagg
     3061 ccgacgagga agaggtccgg cggcggcccg ccacgcagcc cgaaagcggt gtagagggcg
     3121 tcacgctgtg gtcctggcag gccgtccagc cggtccagca gtggccggca gagctggtgc
     3181 accgccgcga acgccaactc catctccgac tgcactcccg ccgcgcccag cacacggcaa
     3241 tcggaagccg ccctcgtcag gtgctcgagc agagcagact tgcccacgcc cgcctcaccg
     3301 cgcaccacca gcgcacggct ctcaccggca cgcaccgccg ccagcagccg gtcgagctgc
     3361 tcgcactcgg cctgccggcc cagtagctgg acaccacgcc gttcggcccg tgcgccaggg
     3421 cggcgggggc tgtcctctga tgtctcgccg tcgcccaccc cgcccgtgac ccgcatgccg
     3481 cggttcgagg tgccaggcat gttctgaaac cgccttccga ttcagctcgg ccgcacgtca
     3541 tcggccggcc aacgtgtccc actggacgag gaccactgcg gtggaggaac gtgctccagt
     3601 gaagccgctc ccgcctcgat ctgatccatc cgctcgacgg tcaccgcaga ctggggcaag
     3661 cgagccgcta cgcgccctcc gacagctgct cgaatcggtc gggcggcatt cgggggccac
     3721 gtgatcactg cacaaccgtc cagcgccatc ggccaacgcc tcccgcgccg actccagtgg
     3781 ctggcgcgtc ccggccgcca cccagcatct cttcgtctga tgcacgcatt attcatcaga
     3841 cgcgagatca cgacgatagg ccgatcatcc tatgtgtcgt gctccggacc cacctgggag
     3901 tagctgtgcg caccttcgat gtcacgctgg atgaaagtcc gtcaggtctg gcggccacca
     3961 gctgcccggg agcgtgcgga ccgagcttcg cgagcgaccg gtgatcatcg acgtccgtcg
     4021 agccgcatac cgcgccagga ccgagaggag tgcgccacgg aatcgacgcc gcgccggatt
     4081 gcctgcctgc tgccgtctgc gcgacaggct ggggtcgtca cggatgcgaa cgtcgtccgg
     4141 tgaagccgac actggcggcg atccgcaacc cgctgaggaa caccaacgcg atgaggaacg
     4201 accgtgccaa cagacgatcg cgtggccgtc cacgacctcg aggacaacca caacgcgacc
     4261 atcgtgctga ccggcgccac ggccgggatc ggccgcgcta ccgcactggt gctcgccccc
     4321 gcagcaggtc atctgatcct ccatggcctg gaacgccagg acgacatcgc ggacctgctg
     4381 cgtgcgctgc gcgccgccat gcgtcccgac acccgcctca cctacgtgcg agcggactac
     4441 agcgacctgg cggacgtgga gcagctcgcc aggcacatcc gctcggcgac cgacggcatc
     4501 gacatcctca tcaacaacgc cgcgcgcccc gggcccccaa ctcggacggt cagcgccgcc
     4561 ggccacgaga tcacgttcca aaccaactac ctggctcccg tcctgctgac cagcaggatc
     4621 atcgacctga tcggcagcgg ggcccggcag gggcggatcg tcaacgtcgc atcggccacc
     4681 cacctgtcgg ccacgctgct cctggacgat ctcgacctag cccaccaccc gtactcggcg
     4741 tctacggcgt acgcgcactc caaactcgcg ctcgtcacgt attcctgctg gctggccgcg
     4801 caccggccca gccgctcact ggatgcggtc agcatgcatc ccggggtgat ctcgaccccg
     4861 ctcctgcacg ccatgttctc cgtcgcaggc gatcgacccg agcacgcggc ggccaacatc
     4921 cactacgtgg cttcgcgccg tgatgacaac ggtacttact acgacgaacg caacccgacc
     4981 ccgcccaacc cgcaggccac cgaccgggca caacaggacc gcctgcacga ggccaccgtc
     5041 cgagccctcc acctctcacc ccgagcccat gtcacgagcg aaaggcagct gccgtcatga
     5101 agccaaaccg ccaagtcatc gccgacgcct tcgccgcgtg ggcggccggc accgg
//
LOCUS       DATACA010000446         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_381155, whole genome shotgun sequence.
ACCESSION   DATACA010000446 DATACA010000000
VERSION     DATACA010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381155"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VHK28_09025"
     CDS             complement(<1..200)
                     /locus_tag="VHK28_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142395.1"
                     /translation="MEAPCAVGSLAPGGAWVGFRRTDEDGYQLVLGEGGSLQVISAEP
                     ALLLAAAITYFEEALPDAPPDL"
     gene            complement(233..1276)
                     /gene="jag"
                     /locus_tag="VHK28_09030"
     CDS             complement(233..1276)
                     /gene="jag"
                     /locus_tag="VHK28_09030"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX2142396.1"
                     /translation="MTFREFNGKNVEEAIRAAMREFEADLSELDIEILSQGSRGILGV
                     GGEEARILAAPKSAIAAAETVRASEPPEVESPPSRRGRPARGRAAEQAPAPEPVPVEA
                     PEAAEAAEVPSPEAITEADDQVAELERAERARGTRGGRTRGARGGRNGRGGDRRDRPA
                     REPREAREREGREQREPLPFIPAKPMEELSERERAILEEAKSVLEEMLRLMEIEASVE
                     VQLGSETAKLNVKGPDLGVLIGRRGEKLASLQHLVNLMAAKSQGQHNRIMVDVENYRG
                     RREEQLRDVAERAAKRVSQSGKIIQLEPMPALERRIVHMALVENARVRTQSVGVEPNR
                     RVVILPANKVGQS"
     gene            complement(1273..2448)
                     /locus_tag="VHK28_09035"
     CDS             complement(1273..2448)
                     /locus_tag="VHK28_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX2142397.1"
                     /translation="MPPTVAAGFLGIEHFTPPWDVFYLPLFNILIAAYQLLFNDFALA
                     IIVFTVIIRTALAPLFVKQIRSQREMQRMQPLIREVQRKHKGNRQKVSEETMALYREH
                     GVNPAAGCLPVILQLPILFALYQAIIRASNVVTVNAQQAASDTFAQVEAALPGIERLR
                     EQGENILYAAPVQGTGEACSLPQFSEAPWSHFLPLNCQLIEPVKLNGAVDTTVGWLFN
                     LDLAQVDNVFALQLGGPEGFAISGLAILAAVLQFVQVKMTSPRPNPDDPTAATSTTMV
                     YLFPLMTIIWGGLFPSGLILYWAIYTGYLVVHQFLMMGWGNLFPIFGWQPKWAPAPDG
                     AAAPPVRRREPEREGKQEASATDAAPNSRPSTNTRSGGAPGGSRQQRAGRKRRGRKR"
     gene            complement(2451..2669)
                     /gene="yidD"
                     /locus_tag="VHK28_09040"
     CDS             complement(2451..2669)
                     /gene="yidD"
                     /locus_tag="VHK28_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX2142398.1"
                     /translation="MRKIGLGMIRGYQRLFAWLPPSCRYTPSCSEYTYVAVERYGLLR
                     GSWMGARRIARCHPLHPGGHDPVPMRKS"
     gene            complement(2666..3001)
                     /gene="rnpA"
                     /locus_tag="VHK28_09045"
     CDS             complement(2666..3001)
                     /gene="rnpA"
                     /locus_tag="VHK28_09045"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX2142399.1"
                     /translation="MPPIPMLRHKADFEALARNGTARSTRLLVLRSLRTGRTETRVGL
                     STPRSLGGAVQRNTVRRRLRGLVRERLETIGPGWDLLLIARPEAATATYAELREALGS
                     LLERSGIGE"
     gene            complement(3019..3153)
                     /gene="rpmH"
                     /locus_tag="VHK28_09050"
     CDS             complement(3019..3153)
                     /gene="rpmH"
                     /locus_tag="VHK28_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX2142400.1"
                     /translation="MKRTYQPKKRARKREHGFRARMASKGGRRVLARRRAKGRKRLTA
                     "
     gene            3427..3588
                     /locus_tag="VHK28_09055"
     CDS             3427..3588
                     /locus_tag="VHK28_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142401.1"
                     /translation="MNGVFVTTSMLVILGSVMTVLGLFGGPQLAVIALGLLAIVSAGA
                     LQVAAARRS"
     gene            3986..5326
                     /gene="dnaA"
                     /locus_tag="VHK28_09060"
     CDS             3986..5326
                     /gene="dnaA"
                     /locus_tag="VHK28_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX2142402.1"
                     /translation="MDAKQVWRAVLGELQVSLSPANFETWLKDTQLVEIDDSRYRIAA
                     PNGFARDWLDNRYRTLVSQTLARVVGGSVQVEFVVADAPGPLPEQADEDRLAMPPTSA
                     GGNTVNLNPRYTFSNFIVGSANRLAHAAALSVAERPGHAYNPLFLYGGVGLGKTHLMH
                     AIGNAVAAKFPRKRILYATSEKFTNDFINSIREQKMEDFRNRYRRIDVLLIDDIQFIA
                     ERERTQEEFFHTFNAIHEAGKQIVLSSDRPPKAITTLEERLRSRFEWGLIADLTPPDL
                     ETRIAILRSKAEDQLNLIPAEVIEFIARKVVSNVRELEGALNRVIAYASMSGMPINIE
                     LASAVLSNVMYNPKKRSITPQRIVRAVADYYGVNLDQLRSSKRDRAIVVPRQIAMFLI
                     REETDISLLRIGAELGGRDHSTVLHACDKIARELAENDEMRREISAVREMIYSE"
     gene            5684..>6025
                     /locus_tag="VHK28_09065"
     CDS             5684..>6025
                     /locus_tag="VHK28_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX2142403.1"
                     /translation="MNVSVMQENLARGLAVVSRAVSTRATLPVLANVLLKTENAGLKL
                     TATNLEIGITCWVPGKVEGEGDITVPARLLTDLVASLPNQRIDLQLSQKDRTLKVTCG
                     VDALDARAAAAA"
BASE COUNT          942 a         2110 c         2020 g          953 t
ORIGIN      
        1 tccaaatcgg gaggcgcgtc cggcagggcc tcctcgaaat aggtgatcgc cgccgccagc
       61 aacaacgcgg gctcggcgct gatcacctgc agcgagccac cctcgcccag gacgagctgg
      121 tagccgtcct catcggtccg gcgaaagccc acccaggcgc cgccgggggc caggctgccg
      181 acggcgcacg gcgcctccat cggtcagtcc cgggcgtcga cctcgggccc cgttaggact
      241 ggccgacctt gttggccggc aggatcacca cccgccggtt gggctccacg cccacgctct
      301 gggtccgtac tcgcgcgttc tcgaccagcg ccatgtgcac gatgcgccgc tccagggcgg
      361 gcatcggctc cagctggatg atcttgccgg actggctgac gcgcttggcg gcacgctcag
      421 cgacatcgcg cagctgctcc tcgcggcggc cgcggtagtt ctcgacatcg accatgatcc
      481 ggttgtgctg gccctggctc ttggcggcca tcaggttgac caggtgctgc aggctggcca
      541 gcttctcccc gcggcgaccg atgagcacgc ccaggtccgg ccccttcacg ttcagcttgg
      601 ccgtctcgct gccgagctgc acctccacgc tggcctcgat ctccatcagt cgcagcatct
      661 cctcgaggac ggacttcgcc tcctccagga ttgcgcgctc ccgctcggag agctcttcca
      721 tcggcttggc cgggatgaag ggcagcggct ctcgctgctc cctgccctcg cgctcgcgcg
      781 cctcgcgcgg ctcgcgggct ggtcgatcgc gacgatcccc gccacgcccg ttgcggccgc
      841 cacgcgcacc ccgggtccgg ccgccgcgcg tcccgcgcgc acgctccgcg cgctcgagct
      901 ctgcgacctg gtcatccgcc tcggtaatcg cctccgggct gggaacctcc gcggcttcgg
      961 ccgcttcggg cgcctcgacg gggacgggct cgggcgcggg ggcctgctcg gcagccctgc
     1021 cgcgcgcggg acggccgcgc ctgctcggcg gcgattcgac ttccggcggc tcggaagctc
     1081 gcacggtctc ggcggcagcg atggcggact tgggcgctgc caggatgcgc gcctcctcgc
     1141 cgcctacccc caggatgccg cggctgccct gggacaggat ctcgatatcc agctcggaga
     1201 ggtccgcctc gaactcgcgc atcgcggcgc gaatggcctc ctccacgttc ttgccattga
     1261 actcacggaa tgtcatcgct ttcggcctcg tcgcttgcgt cccgcacgct gctgccggct
     1321 gccgccgggc gcgccgccgg aacgggtatt ggtggatggt cggctgttcg gcgcggcatc
     1381 ggtcgcgctc gcttcctgct tgccctcgcg ctccggctca cggcgccgca ccgggggagc
     1441 tgccgctcca tcgggcgccg gggcccactt gggctgccag ccgaagatcg ggaagaggtt
     1501 gccccaaccc atcatcagga actgatggac gaccaggtac ccggtgtaga tggcccagta
     1561 caggatcagg ccgctgggga agagcccgcc ccagatgatg gtcatcaggg ggaacaggta
     1621 caccatggtc gtgctggtcg cagcggtggg gtcgtccggg ttcggtcgcg ggctcgtcat
     1681 cttgacctgg acgaactgaa gcaccgcggc caggatcgcc agcccggaga tggcgaagcc
     1741 ctccggcccg ccaagctgga gagcgaagac gttgtcgacc tgggccaggt cgaggttgaa
     1801 gagccagccc acggtggtgt ccaccgcccc gttcagcttc actggctcga tcagctggca
     1861 gttcaggggc aggaagtggc tccacggagc ctcgctgaac tggggcaggc tgcaggcctc
     1921 cccggtgccc tgcaccggcg ccgcgtacag gatgttctcg ccctgctcgc ggaggcgctc
     1981 gatccccggc agcgccgcct ccacctgcgc gaaggtatcg gacgccgcct gctgggcgtt
     2041 caccgtgacg acgttcgagg cgcggatgat ggcctggtag agcgcgaaca ggatcggcag
     2101 ctgcaggatc accggcaggc agccggccgc ggggttgacg ccgtgctccc ggtagagcgc
     2161 catcgtctcc tcggagacct tctggcggtt gcccttgtgc ttgcgctgca cctcacggat
     2221 cagcggctgc atgcgctgca tctcgcgctg agagcggatc tgcttgacga agagcggcgc
     2281 gagcgcggtg cggatgatga cggtgaagac gatgatcgcc agcgcgaagt cgttgaagag
     2341 gagctggtag gccgcgatca ggatgttgaa gagaggcagg tagaagacgt cccagggcgg
     2401 cgtgaagtgc tcgatcccca ggaacccggc agcgaccgtg ggcggcaagg tcagctcttc
     2461 ctcatgggga ccgggtcgtg gccgccgggg tggagcgggt ggcacctggc gatgcgccgg
     2521 gcacccatcc acgatccgcg cagcaggccg tagcgctcga cggccacgta ggtgtactcg
     2581 gagcagctcg gcgtgtagcg gcagctgggg ggcagccagg cgaacagccg ctgatagccc
     2641 cgaatcattc ccaggccgat cttcctcatt ctccgatccc cgaccgctcc aggagcgatc
     2701 caagcgcctc gcgcagctcc gcgtaggtag ccgtcgcggc ctccgggcgc gcgatcagca
     2761 gcagatccca gccgggaccg atggtctcca gccgctcacg caccaatccg cgcagccgcc
     2821 ggcgaaccgt gttccgctgc actgcgcctc ccaggctccg cggcgtgctc agcccgaccc
     2881 ttgtctccgt cctgccggtg cgcagcgatc gcagcaccag cagccgggtc gagcgggcgg
     2941 tcccgttgcg cgcgagcgcc tcgaaatccg ccttgtgccg aagcatcggg atgggcggca
     3001 cgagcgcagc tcgcagcgtc aggccgtgag ccgcttgcgt cccttggcgc gacgtcgggc
     3061 gagaacgcgc cgaccgccct tcgaagccat gcgggcgcgg aagccgtgtt cccgcttccg
     3121 ggcgcgcttc ttgggctgat aggtcctctt catcggtgcg tgattcgtcc tcagtacagc
     3181 ggcgggcggt cagaaccggt ccgaaacata cgaacggctg gcaccaggcc agccttacgc
     3241 gacgccgtca cccggctcgt ctcgctctgc gcggcgattc taggcccgtg ctcgaagggg
     3301 tgtcaaacca gcgggacact tgtcgcacgc tcgtccgcga cgtttgcggc gcgtgggctt
     3361 gcccgccgcg aagacacaat cggctcatcc gtagacgctt gatcggggga ggaagggaga
     3421 aaccccatga acggggtgtt cgtcacgacc agcatgctgg tcatcctggg ctcggtgatg
     3481 accgtgctcg gcctgttcgg cggcccgcag ctggcggtca tcgcgctcgg cctgctggcc
     3541 atcgtctccg ccggcgcgct gcaggtggct gccgcgcgac gcagctgacg ggcgctggcg
     3601 cagcgccgcc accggggcgc taccggggcg ctacccgata catcgggccg ttcttcgcat
     3661 gtccgcccgc ctcggtgtca gatcgcggat gtatcgggca actccacgct ccagccgccg
     3721 agaacttcgc cctttgcgcg tacgcctgcc cgatatttca gcagaacggc acccagatcg
     3781 cgatccccgg gaacttctgc ccgatatatc agcagaccgg gtcggccggg cagcgatccg
     3841 ttgccgcggc cggagaggcg ctgctagtat ggcccccaac ccccgcggcc gacccgccct
     3901 aaacgccggg ctccagcggc ccggagggag taggattccc ggtccctaaa cccctccgac
     3961 cacgagcacc tggagtcggc gattgatgga tgcgaagcag gtctggcgtg ccgtcctcgg
     4021 tgagctgcag gtcagtctct caccggccaa cttcgagacc tggctgaagg acacgcagct
     4081 cgtcgagatc gacgacagcc gctatcgcat cgccgctccc aacggttttg cccgcgactg
     4141 gctggacaac cgataccgca ccctggtgag ccagaccctg gcccgggtgg tgggcggatc
     4201 ggtccaggtc gagttcgtgg tggcggatgc cccgggcccg ctccccgagc aggcggacga
     4261 ggaccggctg gccatgccgc ccacctcggc cggcggcaac acggtcaacc tgaacccgcg
     4321 ctacacgttc agcaacttca tcgtgggcag cgccaaccgg ctggcgcacg ccgcggccct
     4381 cagcgtcgcg gagcgccccg gccacgccta caacccgctc ttcctgtacg gcggggtggg
     4441 gctgggcaag acgcacctga tgcacgcgat cggcaacgcg gtggccgcca agttcccgcg
     4501 caagcggatc ctgtatgcca ccagcgagaa gttcaccaac gacttcatca actccatccg
     4561 cgagcagaag atggaggact tccggaaccg ataccgccgt atcgacgtgc tcctcatcga
     4621 cgacatccag ttcattgccg agcgcgagcg gacgcaggaa gagttcttcc acaccttcaa
     4681 cgctatccac gaggcgggca agcagatcgt gctctcctcg gaccggccgc cgaaggccat
     4741 caccaccctg gaggagcgcc tccgctcccg gttcgagtgg ggcctgatcg ccgacctgac
     4801 gccgccggac ctggagacgc ggatcgccat cctgcgctcc aaggcggagg accagctgaa
     4861 cctgatcccg gccgaggtga tcgagttcat cgcccgcaag gtggtcagca acgtccgcga
     4921 gctggagggc gccctcaacc gggtcatcgc ctacgcctcc atgtcgggca tgcccatcaa
     4981 catcgagctg gcctcggcgg tcctttccaa cgtcatgtac aaccccaaga agcggtcgat
     5041 caccccgcag cgcatcgtgc gcgcggtggc cgactactac ggggtcaacc tggaccagct
     5101 gcgaagctcg aagcgcgacc gggccattgt ggtgccgcgc cagatcgcca tgttcctcat
     5161 ccgtgaggag accgatatct cgctgctgcg catcggtgcc gagctcggcg ggcgtgacca
     5221 ctccaccgtc ctgcacgcct gcgacaagat cgcccgggag ctggccgaga acgacgagat
     5281 gcggcgggag atcagcgcgg tgcgggagat gatctactcc gagtgagccg ggccgcaaag
     5341 tgcggccttt ggtggataac cggcccgatc cggtggatat cggtgggtga ctgtggacaa
     5401 atcggccccc gctcggcccc gcgaagccgc agccttatgc accgatgcca ccgctgagcg
     5461 caccatcggt caagaacggg cgcccggccg tccaccatcg gtgacgactt atgcgcagcc
     5521 gctgagagca gggattgagc acgacaggca cgggccttcc acaccgtcca cacccctgcg
     5581 agagacggtg acggaatggg tattcttcta gaggtttcgt atataggcga ggagcaccgt
     5641 ggaaagtttg gtcggtgcgc ggatcggtga gggtgaggtc tcgatgaacg tctcggtgat
     5701 gcaggagaac ctggcacgcg gcctggcggt ggtcagccgc gcggtcagca cccgtgccac
     5761 cctgccggtg ctggccaacg tcctgctcaa gacggagaac gcgggactga agctgaccgc
     5821 caccaacctg gagatcggga tcacctgctg ggtgccgggc aaggtggagg gggagggcga
     5881 catcaccgtg ccggcccggc tgctgaccga cctggttgcc tcgctgccca accagcggat
     5941 cgacctgcag ctctcgcaga aggaccgcac ccttaaggtc acctgcggcg tcgatgccct
     6001 tgatgctcga gcggctgccg ccgca
//
LOCUS       DATACA010000447         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2069229, whole genome shotgun sequence.
ACCESSION   DATACA010000447 DATACA010000000
VERSION     DATACA010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2069229"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VHK28_09070"
     CDS             complement(<1..1158)
                     /locus_tag="VHK28_09070"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX2142404.1"
                     /translation="MTQGPGWGIEPSYRDAWKRRRRIASPVRRSIEAALGSEPADTGA
                     GPVAVVPPGGSLPAAGEVTLEDGTRLGRLAALPPDAPFGYHRLEVEGGAFPTRLITAP
                     EACHLPSQRSWGWSVQLYAARSRQSWGIGDLADLRTLGEWASSLGAGMLIVNPLNAPA
                     PVPPIQPSPYFPGTRRFRSPLYLRVEEVPGASSLGGRRLETLGAAGRALNDRELIDRD
                     AAFGAKMAALEMLWRAGAPRRGLGRYRREQGAALRSWARYAVIAERHGASWSRWPEEL
                     RRPSSRAVARFAADHADRVAFHEWIQWLLDQQLARAGSALPLIADVPIGVDREGADAW
                     AWQEAMALDVNIGAPPDLFNRAGQDWGLPPFNPAALRAAGYEPFIQTLRAAL"
     gene            complement(1155..2885)
                     /gene="treZ"
                     /locus_tag="VHK28_09075"
     CDS             complement(1155..2885)
                     /gene="treZ"
                     /locus_tag="VHK28_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX2142405.1"
                     /translation="MSVPRLAVWAPNAERVEACIGGGRLAMTPQEGGWWQLPREMTAG
                     TDYGFSLDGGPSLPDPRSPWQPSGIEGPSRTLDHDAFAWTDAGWRAPPLGAGAVYELH
                     VGTFTPGGTFDTAAERLADLAALGVTHLELMPVAEFSGRHGWGYDGVDLYAPHHAYGG
                     PEGMKRFVDAAHAHGLAVICDVVYNHLGPAGNHLAKFGPYFTDRYSTPWGSAINYDDR
                     GSEEVRRFVIDNALMWLRDYHADGLRLDAVHTIIDTSALHILEEIGHEVRRLELAVGR
                     PLLVTVETDRNDPRLVRSTDAGGLGLDAAWNDDFHHALHALLTGERDGYYADFGEMKH
                     LAEALQHGWVFRGGFSAERERRHGREPMGVPGVRFIGYSQNHDQVGNRARGERISQLV
                     GIGRAKIAAAVVLTSPFVPLLFAGEEWAASTPFPYFSDHADPQLAEAVRTGRQEEFAA
                     FGWDSSSIPDPQDPATFQAAVLRWEERDRGTHRDMLEWYRALLALRQVTPALLDPRLT
                     QVDCDHEPEAGWFAIRRGGISIIFNLGPEPVTLPASGELLLASGPRVAMDGEGLRLPP
                     EAVAIVGSRP"
     gene            complement(2882..>4343)
                     /locus_tag="VHK28_09080"
     CDS             complement(2882..>4343)
                     /locus_tag="VHK28_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HEX2142406.1"
                     /translation="TGAPTGWTEVAREARLLTLSASLGSDVNRLTELWLTICEAHRRY
                     RDFTRHELHEALQEVAAAFGVYRTYVRPGSGQVEPADRAAVEAAVGKAEELRQDLDPD
                     LFAFLRAILLLEVNGPEAAELAARFQQLTPPAMAKGVEDTAFYRYLRLVALNEVGGDP
                     GRFGVTPDEFHAVLVRDAARWPRAMLATSTHDTKRSEDVRARLALLSEMPDAWREAVE
                     RLGRRAAPHRSGADVPGPHAEYLFFQTLVGAWPIDADRAAAYMQKAAREAKERTSWTS
                     PDADFEAALDSLVRGCLADPAFLAELEAVVAPLVEPGRVNALAQLLCKLAAPGVPDVY
                     QGTELWDLSLVDPDNRRPVDFETRASLVGGLGDAPPRLADDGRAKLWLLQRALQLRAR
                     RPGDLGSQGGYRPLAAHGPSASHVMAWLRGDGVVAVAQRLPLGLAASGGWRGTVLPLP
                     QGRWCNRLGRDAPHAGEAALDELLAPFPVALLELVG"
BASE COUNT          609 a         1597 c         1574 g          563 t
ORIGIN      
        1 cagggcggcg cgcagcgtct ggatgaacgg ctcgtagccg gcggcgcgca gggctgccgg
       61 gttgaacggc ggcaggcccc agtcctgccc ggcgcgattg aagaggtccg gcggggcgcc
      121 gatgttgacg tccagcgcca tggcttcctg ccaggcccag gcatcggccc cctcgcggtc
      181 gacgccgatg ggcacgtccg cgatcagcgg cagcgcactg ccggcacgag ccagctgctg
      241 atcgagcagc cactggatcc actcgtgaaa ggcgacccgg tccgcgtggt cggccgcgaa
      301 gcgcgccacc gcgcggctgg atggacggcg cagctcctcc ggccagcgac tccacgacgc
      361 cccgtgccgc tcggcgatca ccgcgtagcg cgcccaggac ctcagcgccg cgccctgctc
      421 gcggcggtag cggcccagtc cgcggcgtgg cgcacccgcg cgccagagca tctccagcgc
      481 cgccatcttg gccccgaagg ccgcgtcacg gtcgatcagc tcccggtcgt tgagcgcgcg
      541 gccggcggca cccagcgtct ccaggcgacg accgcccagg gacgaggctc cgggcacctc
      601 ctccacccgg aggtagagcg ggctgcggaa gcggcgcgtc ccgggaaagt aggggctggg
      661 ctggatgggc ggcacgggtg ccggcgcgtt gagtgggttc acgatgagca tcccggcgcc
      721 caggctggag gcccactcgc ccagcgtccg caggtcggcc aggtcgccga tcccccagct
      781 ctgccgcgag cgggccgcgt agagctgcac cgaccagccc cagctgcgct gcgacggcag
      841 gtggcaggcc tccggagcgg tgatcaggcg ggtgggaaag gcgccgcctt ctacctcgag
      901 ccggtggtag ccgaacgggg cgtccggcgg cagcgcggcc agcctgccca gccgcgtgcc
      961 gtcctccagg gtcacctcgc cggctgccgg cagcgaaccg ccgggtggca ccacggccac
     1021 ggggccggcg ccggtgtcgg ccggctcgct gccaagcgca gcctcgatgc tgcggcgcac
     1081 cggagatgct atccgtcgcc ggcgcttcca ggcgtcgcgg tacgacggct cgattcccca
     1141 ccccggcccc tgggtcacgg gcggctcccc acgatggcga ccgcctcggg cggcagcctc
     1201 agcccctcgc cgtccatcgc gactcgcggc ccggaggcca acagcagctc cccggaggcc
     1261 ggcagcgtta ccggttcggg gcccaggttg aagatgatcg agatgccgcc gcggcggatg
     1321 gcgaaccagc cggcctccgg ctcgtggtcg cagtcgacct gggtcagccg cgggtcgagc
     1381 agggcggggg tgacctggcg cagcgcgagc agggcgcggt accactcgag catgtcgcgg
     1441 tgcgtgcccc ggtcacgctc ctcccagcgc aggacggccg cctggaaggt ggccggatcc
     1501 tgtgggtccg ggatcgacga cgagtcccag ccgaaggcgg cgaactcctc ctgccgtccg
     1561 gtccgcaccg cctcggccag ctgcgggtcg gcgtggtcgc tgaagtacgg gaagggagtc
     1621 gaggcggccc actcctcccc ggcgaagagc agcggcacga agggcgaggt gaggaccacg
     1681 gcggccgcga tcttcgcacg accgataccc accagctgcg agatgcgctc tccccgtgcg
     1741 cggttgccga cctggtcatg gttctgggaa tagccgatga atcgcacgcc ggggacaccc
     1801 atcggctctc ggccgtggcg ccgctcccgc tccgccgaga atccgccgcg gaagacccag
     1861 ccatgctgca gtgcctccgc caggtgcttc atctccccga agtcggcgta gtagccgtca
     1921 cgctcgccgg tcagcagcgc gtggagcgcg tgatggaagt cgtcgttcca ggcggcgtcc
     1981 aggccgagcc cgccggcatc ggtggagcgc accagccgcg ggtcattgcg gtccgtctcc
     2041 accgtcacca gcagcggcct gccgacggcc agctccaggc ggcgcacctc gtgcccgatc
     2101 tcctccagga tgtggagcgc cgaggtgtcg atgatggtgt gcaccgcgtc caggcgcagc
     2161 ccgtcggcgt ggtagtcgcg cagccacatc agcgcgttgt cgatgacgaa gcggcgtacc
     2221 tcctccgatc cgcggtcgtc gtagttgatg gcactgcccc agggagtcga gtatcggtcg
     2281 gtgaagtagg gcccgaactt cgccaggtgg ttgccggccg gaccgagatg gttgtagacg
     2341 acgtcgcaga tgaccgccag gccgtgggcg tgggctgcgt cgacgaagcg cttcatgccc
     2401 tccggcccac cgtaggcgtg gtgcggtgcg tagaggtcaa ccccgtcgta accccagcca
     2461 tgccgtccgc tgaactcggc caccggcatc agctccaggt gggtgacgcc gagcgccgcc
     2521 aggtccgcca gccgctcggc ggccgtgtcg aaggtgccgc cgggggtgaa tgttccgacg
     2581 tgcagctcgt acaccgcgcc ggcgcccagc ggtggcgcgc gccagccggc atcggtccag
     2641 gcgaaggcgt cgtggtcgag cgtgcgggat ggtccttcga tgccggacgg ctgccacggg
     2701 gagcgagggt cgggcagcga ggggccgccg tcgagcgaga agccatagtc ggtcccggcg
     2761 gtcatctccc gtggcagctg ccaccagccg ccctcctgcg gggtcatggc cagccgcccc
     2821 ccgccgatac aggcttcaac ccgctcggcg ttgggcgccc acaccgccag ccgcggcacg
     2881 ctcatccgac aagctccagc agggcaaccg ggaagggcgc cagcagttcg tccaacgctg
     2941 cctcgccagc gtgcggggca tccctgccca ggcggttgca ccagcggcct tgcggcaggg
     3001 gcagcacggt gccacgccaa ccgccggagg cggccagccc aagcggcagg cgctgggcga
     3061 ccgccaccac gccgtcgccg cgcagccagg ccatcacgtg gctcgccgac gggccatggg
     3121 ctgccagcgg ccggtagcca ccctgggaac ccaggtcacc cggacggcgc gcgcggagct
     3181 ggagcgcgcg ctgcagcagc cacagcttgg cgcggccatc gtctgcgagg cgcggaggag
     3241 cgtcacccag cccacccacg aggctcgcac gcgtctcgaa gtcgaccggg cgccggttgt
     3301 ccgggtccac cagcgacagg tcccagagct cggtcccctg gtacacgtcc gggacgccgg
     3361 gcgcggccag cttgcagagc agctgggcca gcgcattcac ccgtcccggt tcgacgagcg
     3421 gcgcgacgac ggcctccagc tcggccagga aggccggatc ggccaggcag ccccgcacca
     3481 gcgaatccag tgccgcttca aagtcggcgt ctggcgaggt ccagctggtg cgctccttgg
     3541 cctcgcgcgc cgccttctgc atgtaggccg ccgcgcgatc ggcgtcgatc ggccaggcgc
     3601 cgaccagggt ctggaagaag aggtactcgg cgtgtggacc ggggacgtcc gccccggacc
     3661 gatgcggcgc tgcgcgccgc ccaaggcgct ccaccgcctc gcgccaggcg tcgggcatct
     3721 cggacagcag cgccagtcgg gcacgcacat cctcgctgcg cttggtgtca tgcgtggagg
     3781 tggccagcat ggcgcgcggc cagcgcgccg catcgcgcac cagcaccgcg tggaactcgt
     3841 cgggcgtgac gccgaaccgc cccggatctc cgccgacctc gttgagagcc accagtcgca
     3901 ggtagcggta gaaggcggtg tcctccacgc ccttggccat ggccggtggc gtcagctgct
     3961 ggaagcgggc ggccagctcc gcggcctccg gtccgttgac ctcgagcagc aggatcgccc
     4021 gcaggaaggc gaacaggtcc gggtccaggt cctggcgcag ctcctcggcc ttgcccaccg
     4081 ccgcctccac cgccgcgcga tcggcgggct cgacctggcc gctcccgggt cgcacgtagg
     4141 tgcggtacac cccaaacgcg gcggccacct cctgcagcgc ctcgtgcagc tcgtggcggg
     4201 tgaagtcgcg gtaccggcgg tgcgcctcgc agatggtgag ccacagctcg gtcaggcggt
     4261 tgacgtcgct gcccagcgac gcggacagcg tcagcagccg tgcctcgcgc gctacttcgg
     4321 tccagccagt gggagctccg gtg
//
LOCUS       DATACA010000448         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1426548, whole genome shotgun sequence.
ACCESSION   DATACA010000448 DATACA010000000
VERSION     DATACA010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426548"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1717
                     /locus_tag="VHK28_09085"
                     /pseudo
     CDS             147..1717
                     /locus_tag="VHK28_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
     gene            complement(1714..>2045)
                     /locus_tag="VHK28_09090"
     CDS             complement(1714..>2045)
                     /locus_tag="VHK28_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142407.1"
                     /translation="GEPVIVLQSDEGPWPPRFSADQTDFQWLEATDAEIDWKYSILNA
                     LRLPGVDAREAGFDNSISPVNEFRVVFNALFGSNLPLLPDRYYLSPDYDHMWSLVEYE
                     RPDPPVF"
BASE COUNT          319 a          762 c          674 g          290 t
ORIGIN      
        1 atcggtctag actcctcgtc cgggctcgcg cgtcagacgg gcagtgccca gccagtcagg
       61 aggatcacgt gagcagcacc ttcgccgacc ttgccagccg gttcctcgac gagcagtacg
      121 ccgaacatcc cgtccgcgcc agcggtatgg gcctcaccgc cttcgacgac cagctggacg
      181 acctgtccgc cgccgccttc gagcgtcgcg cggatgccgc ccgcggctgg cgggagcgat
      241 tcgccgcgct cccagcgggc gagctgacat tcgacgagac gatcgatcgc gacctggtgc
      301 tcgccgcact ggacgagcac gagatctacg acgagtggga gaactggcgc cggcacccgg
      361 acatctacct caacccgggc atgcgcggtg tcttcatcct gttcctgcac atgctgcgcc
      421 ccgaggcgga gctggccgcc gccgccgcca gccggctgcg ccaggtgccg gccaacctgg
      481 aggcgggccg cgccaacctg cggccagagc tggtgccgcc cattctgctc gaacgcgccg
      541 tgaaccaggc acgggccggc gccacctaca cccgtcacct gctgcccgcc caggtggccg
      601 aggagaaccg ggctgacttg gccgatgccg gcgccgctgc ggccgacgcc ttcgaggagt
      661 acgccgcctt cctggaggac atgcggggat cggcgcaggg cgactgggcc ttcggccagg
      721 accgctacga cggcgtgctg cgccgcggcg agctgctctc cttcgacgcc cgggcactgc
      781 gcgagactgg gcgccagcag ctggaggagc tgaccgtcgc gctgcgaacc acggcccgcc
      841 agatcgccgg acacgaggac tggcacgccc tgctgctgga gctcaacaag gatcggccgg
      901 cgacgccgga ggacatgcgc cgcgggtacg aggactggac cgagcgcgcc cgcaccttcc
      961 tccacgagcg ccgcctggtc agcttcccgc caggcgagga gtgccacgtc gtgccctcgc
     1021 cgccctacca gcggccggtg ctggccgtcg cctcctacgt cgccccgccc agctttgcgg
     1081 agacgatgcg cggtcacttc ttcgtgccct acccgccgga cggtgccagc gaggaggaga
     1141 tcggcaagcg actggagtcc aactgctatc ccggcatccc gaccaccgcc gtccacgagg
     1201 cgtacccggg ccaccactgg cacctggtca tggccaaggg caatccctcg cacctgcggc
     1261 agtggtacgg caccagctac ttcgcggagg gctgggcgct ctacgccgag cggatgatgg
     1321 aggacgccgg cttctacgac gacccgcgcc acgtcctcta ccggctggag gccacgctct
     1381 tccgtgcggc gcgcatcgtg ccgacaccag cctgcacctc gggcagatga gccacgagga
     1441 ggcggtgcag ttcatgatgg ccaactcgtc gctgaccgag ccgacggcca ccgccgaggt
     1501 cacccgctac tgcagctggc cgacccaggc gtcctcgtac ctcaccggct gcctggagat
     1561 cctgcgcatc cgggaccgat acctctcagc ccgctggacc gatgcaaccg acgtcgacgc
     1621 cctgcgcgcc ttccacgacg tcatcgcctc cgccggcacc ctgcccatcg ccctggcaga
     1681 gcgggccgtc atggcgtccc tggacgggat gggctagaag accggcggat ctggccgctc
     1741 gtactcgacc agcgaccaca tgtggtcgta gtccggcgag aggtagtagc ggtccgggag
     1801 cagcggcagg ttcgagccga acagggcgtt gaatacgacc cggaactcgt tcaccgggga
     1861 gatgctgttg tcgaacccgg cctcgcgcgc atccacgccc ggcagccgca gcgcgttcag
     1921 gatcgagtat ttccagtcga tctccgcgtc cgtagcctcc agccactgga agtcggtctg
     1981 gtcggcgctg aagcgcggag gccacgggcc ctcgtccgac tgcagcacga tcaccggctc
     2041 cccct
//
LOCUS       DATACA010000449         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1266136, whole genome shotgun sequence.
ACCESSION   DATACA010000449 DATACA010000000
VERSION     DATACA010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266136"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VHK28_09095"
     CDS             complement(<1..790)
                     /locus_tag="VHK28_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEX2142408.1"
                     /translation="MTRTAPAADLAPGAAADPGTLRALEFGAVIEELSSLAAFGPSRE
                     MAEASLPVADAAHVALLQEQTDEAARLLDEQAQATIGGARDIRAALQRAERGGRLTAG
                     DLLDIAETCRATALFASRLGSWRGAHLAGLRDELDAAPALRERIERSVDEAGEVLDTA
                     SPELAAIRKRMRTAQDRVRDRLNAILRSSDLAGAIGEPIVTVRAGRYVIPVRAEAKGR
                     VKGIVHDQSASGATLFVEPLAVVELNNTWTTAALDAAREEERILD"
     gene            complement(765..1370)
                     /locus_tag="VHK28_09100"
     CDS             complement(765..1370)
                     /locus_tag="VHK28_09100"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2142409.1"
                     /translation="MDLGERPAKASVDRAWFDRPATDVARDLLGCTLVHDSADGRVAG
                     RIVEVEAYQGPEDRAAHSWRGPTPRNAVMFGPPGHLYVYLIYGLHQCANVVCGPGTKP
                     EAVLLRAAEVTEGLGLARRRRGPVPDRRLAAGPGNLGAAFGFDRGLNGADLLDGPVRI
                     LPGTPALNVTVTPRIGVAYAGAWADRPLRFLVTDDPHRSRR"
     gene            complement(1403..1972)
                     /locus_tag="VHK28_09105"
     CDS             complement(1403..1972)
                     /locus_tag="VHK28_09105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX2142410.1"
                     /translation="MEFFTRLSPIDLFIVAALAAGVFAGFTQGLIRYALNILVVPIAF
                     VIASQLKGPLDSALSFWEAFTPDLREQILFLLLFIGLVVGGWFIIRALYKRTRLPIVR
                     QLDELGGAFLGLIFAVVSIVFLLVTMDSFFLNVPDSQAADAGILKGFYDAMSDSVLVG
                     VFRDSVIPTLGFLTRPFVPEEIAQLLEPQ"
     gene            complement(2113..>2381)
                     /locus_tag="VHK28_09110"
     CDS             complement(2113..>2381)
                     /locus_tag="VHK28_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2142411.1"
                     /translation="IDRDLAVIVDDAAPVGELLRLVRLNAGSMLVEARIFDVYRGEQV
                     GAGRVSYALALRFQPESAGDEKGVERALNKVRGSLRHHLGAEIR"
BASE COUNT          371 a          845 c          863 g          302 t
ORIGIN      
        1 cgtccaggat gcgctcctcc tcgcgggcgg cgtccagggc ggccgtggtc caggtgttgt
       61 tcagctcgac gacggccagc ggctcgacga acagggtggc gcccgaggcg ctctggtcgt
      121 ggacgattcc cttcacccgg cccttcgcct cggcgcggac ggggatcacg tagcgcccgg
      181 cccggacggt cacgatcggt tcaccgatgg ccccggccag gtcgctggag cgcaggatgg
      241 cgttgaggcg atcgcgcacc cggtcctggg cggtgcgcat ccgcttgcgg atggcggcca
      301 gctcgggcga cgccgtgtcc agcacctcgc ccgcctcgtc cacgctgcgc tcgatgcgct
      361 cgcgcagggc cggggccgcg tccagctcgt cgcggaggcc ggccaggtgc gccccccgcc
      421 aggacccaag gcgggaggcg aagagcgcgg ttgcccggca cgtctcggcg atgtccagga
      481 ggtcgcccgc cgtcagccgc ccgccccgct cggcgcgctg cagggcggca cgaatgtctc
      541 gcgccccacc gatggtcgcc tgcgcctgct cgtcaagcag ccgcgcggcc tcatcggtct
      601 gctcctggag aagcgcgacg tgcgctgcat cggcgaccgg cagggatgcc tctgccatct
      661 cgcgggaagg gccgaaggcg gccagggacg aaagctcctc gatcaccgcg ccgaactcga
      721 gcgcgcggag tgtgccgggg tcggcggccg ccccgggggc caggtcagcg gcgggagcgg
      781 tgcgggtcat cggtgaccag gaagcggagc ggacgatccg cccaggcgcc ggcgtaggcg
      841 accccgatgc gcggggtgac agtcacgttc agcgccggag tgcctggcag gatgcgcacc
      901 ggtccatcca gcagatccgc gccattcagg ccgcggtcga acccgaaggc agcacccagg
      961 ttccccgggc cggcggccag gcgccgatcg ggtaccggtc cccgccgtcg tcgggccagg
     1021 cccagtccct cggtgacctc cgcggcgcgc agcaggacgg cctccggctt ggtcccgggg
     1081 ccgcagacca cgttggcgca ctggtgcagg ccgtagatga ggtacacgta gaggtgtccc
     1141 ggcgggccga acatgacggc gttgcgcggc gtcgggccgc gccaggaatg cgcggcgcgg
     1201 tcctcagggc cctggtaggc ctccacctcc acgatgcggc cggccacccg gccatcggct
     1261 gagtcatgca cgagcgtgca gccaagcagg tcgcgagcca cgtccgtcgc cgggcggtcg
     1321 aaccaggcgc ggtccactga cgccttggca ggacgctcgc cgaggtccac gccgccagcc
     1381 gatgctccgg ccgcggccgg gctcattgcg gttcgaggag ctgggcgatc tcctccggca
     1441 cgaacggccg cgtcaggaag ccgagggtcg gaatgacgct gtcccggaat acgcccacca
     1501 gcaccgagtc gctcatggca tcgtagaagc ccttcaggat gccggcgtcg gccgcctgcg
     1561 agtcaggcac gttcaggaag aagctgtcca tggtgaccag caggaagacg atgctgacca
     1621 ccgcgaagat caggcccagg aaggcgccgc ccagctcgtc cagctggcgc acgatgggca
     1681 gccgggtccg cttgtagagg gcgcggatga tgaaccagcc gccgaccacc aggccgatga
     1741 acaggagcag gaacaggatc tgttcgcgca ggtccggtgt gaaggcctcc cagaagctca
     1801 gcgccgagtc caggggaccc ttcagctgcg aggcgatcac gaaggcgatc ggcaccacca
     1861 ggatgttcag cgcgtagcgg atgaggccct gggtgaagcc cgcgaagaca ccggccgcca
     1921 gcgccgcgac gatgaacagg tcgatcgggc tcagtcgggt gaagaactcc acgggatgca
     1981 cctcctcctg atcaggctcg gagcgatccg gccggaggaa ccggggctcg ggcgcgcgaa
     2041 gggtagcagc cagcgtcggg cacggacaac ccgcgccgac caagcgtgcg ggcgggccgc
     2101 tggaatccgc tcctagcgga tctccgcgcc caggtgatgg cgaagcgagc cgcgcacctt
     2161 gttcagcgcc cgctccacgc ccttctcgtc gcccgccgac tctggctgga agcggagcgc
     2221 cagggcgtag gagacgcgac cggctcccac ctgctcgcct cggtagacgt cgaagatcct
     2281 ggcctccacc agcatagacc cagcgttcag acgcaccagc cgcagcagct cgccgaccgg
     2341 cgccgcgtcg tcgacgatca ccgccaggtc gcggtcgatg g
//
LOCUS       DATACA010000450         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_784207, whole genome shotgun sequence.
ACCESSION   DATACA010000450 DATACA010000000
VERSION     DATACA010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784207"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VHK28_09115"
     CDS             complement(<1..305)
                     /locus_tag="VHK28_09115"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX2142412.1"
                     /translation="MAHANGNGTVSDWFRRAAHEVTLWSGSWQFFLFSVVGILAWAAL
                     GPPMTYSDTWQLIVNTPTTVLTYLLGILILMEANRQSRESKIVHDELLRAIRNARDEL
                     "
     gene            454..816
                     /locus_tag="VHK28_09120"
     CDS             454..816
                     /locus_tag="VHK28_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX2142413.1"
                     /translation="MSRLLVHLASGPEHPTRAALALLVARTAAQQGHDVDVFLAGDAV
                     GLLRDATMDAVQGIGTGSLREHFDALAAANVRFYASGMSSKARGVDADALGGKQVEFS
                     PPDKLVELTFAADRVLTY"
     gene            816..1157
                     /locus_tag="VHK28_09125"
     CDS             816..1157
                     /locus_tag="VHK28_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX2142414.1"
                     /translation="MLLAPLGAIAGVLSGLLGIGGGVVMVPALMTVGRLDPHRAHAAS
                     LAATVPIALLGAVLYAIGGEIDFLAAGLLVAGSLAGVRAGTSVMQRMDAVWLTRAFAL
                     LLLAVAVTMLL"
     gene            1154..1570
                     /locus_tag="VHK28_09130"
     CDS             1154..1570
                     /locus_tag="VHK28_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2142415.1"
                     /translation="MTFEIALALVGVGLLAGFLAGLFGIGGGVVLVPALVLLLDFDQH
                     AAQGTSLLVIVPAAVLGSWTHHRQGRLALPQAVLLAAGGVVGTVLGGAVALSLDEEIL
                     RRIFAIVVVVMGIRLLLPRRRTNPPVSEAPEGPTAA"
     gene            1631..2611
                     /locus_tag="VHK28_09135"
     CDS             1631..2611
                     /locus_tag="VHK28_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX2142416.1"
                     /translation="MRASRLVSVLLLLQTRGRMTAGELARELEVSERTIYRDIDALAE
                     SGVPIYAERGPLGGVQLVEGYRTRLTGLTADEAEALFLSGLTGPAAELGLGTVVAAAQ
                     LKVRAALPTELRSRASRLVERFHLDASSWFQPGEAVEHLTVIAQAVWDTRTLEIAYDR
                     GDRLVERTVEPLGLVLKGGTWYLVGRVDGQLRTYRASRIVSVEPTDTVFERPAHFDLA
                     GYWQESISAYERETPRLEMTIRVAPGRTGSLSWVLGDDALSTAEREPGPDPEGWVRLR
                     VKVLWPEDAAGRLLSMGGDLEVVDPPEVREELLALARSALDRHVSPPVAG"
BASE COUNT          380 a          950 c          984 g          457 t
ORIGIN      
        1 agctcatccc tggcgttgcg gatggctcgt agcagctcgt cgtgcacgat cttcgactcg
       61 cgtgactgcc ggttggcctc catcaggatc aggatgccga gcaggtaggt cagcacggtg
      121 gtcggcgtat tcacgatcag ctgccatgtg tcgctgtagg tcatgggcgg gccgagggcc
      181 gcccaggcca ggatgcccac caccgagaac aggaagaact gccaggagcc gctccagagc
      241 gtcacctcgt gagcggcgcg ccgaaaccag tcgctgacgg tgccgttgcc gttcgcatgc
      301 gccacgttcg tctctcccga gcaaccggcc cagcctgaac gggccgcctg gcgcgagaca
      361 atgcacgtcg cttgccgaga aggcgctcgg ccgctcgtac cagacaggca ccatacgtgc
      421 gtcaaccatc gcctaccccg aacggagaga ccgatgagcc gactcctggt tcacctggcc
      481 agcgggcctg agcatcccac gcgggcggca ctcgcgctcc tggtggcgcg cacggccgcg
      541 cagcaggggc acgacgtgga cgtcttcttg gccggcgatg ccgtcgggct cctgcgcgac
      601 gccacaatgg acgccgtgca gggcatagga accggcagcc tgcgcgagca cttcgacgcg
      661 cttgccgcgg ccaatgtccg gttctacgca tcgggcatgt caagcaaggc ccgcggggtg
      721 gatgccgacg ccctgggtgg caagcaggtc gaattctcgc cacccgacaa gctggtcgag
      781 ctcacgttcg ccgccgaccg ggtgctgacc tactgatgct gctcgccccc ctcggtgcca
      841 tcgccggcgt gctcagcgga ctgctgggga tcggcggcgg ggtggtcatg gtcccggcgc
      901 tgatgacggt cgggcggctg gatccgcacc gcgcccacgc ggcgtcgctg gcggcgaccg
      961 tgccgatcgc gctgctcggc gctgtgctct atgcgatcgg cggcgagatc gactttctcg
     1021 ccgccggcct gctggttgcc ggcagccttg ccggcgtgcg ggccgggaca agcgtcatgc
     1081 agcgcatgga cgccgtctgg ctgacgcgcg ctttcgccct gctgttgctg gcggtggcgg
     1141 tcaccatgct gctgtgacgt tcgagatcgc cctggccctg gtgggagtag ggctcttggc
     1201 ggggttcctg gcaggcctat tcgggatcgg cggcggggtt gtactggtgc ccgcgctggt
     1261 cctgctgctg gacttcgatc agcacgccgc ccagggcacc tcgctgctgg tcatcgtgcc
     1321 ggccgccgtg ctgggctcgt ggacccacca ccggcagggc cggctggcac tgccccaggc
     1381 cgtgctgctc gccgcgggcg gcgtggtggg caccgtcctg ggaggtgcgg tggcactctc
     1441 cctggacgag gagattctgc gcaggatctt cgcgatcgtg gtcgtggtga tgggcatccg
     1501 gctgctgctc ccgcgccggc gaaccaaccc gccggtatcg gaggcccctg aaggcccaac
     1561 cgccgcctga ggcgcctcct cgctctattc ctgccgtgat gtgtcaggaa tatcggtcta
     1621 tcctgctcgg gtgcgagcgt cgcgcctggt gtcggtgctg ctgctgcttc aaacccgcgg
     1681 acggatgacc gccggggagc tcgcgcgcga gctcgaggtg tcggagcgaa ccatctaccg
     1741 cgatatcgac gcgctcgccg aatcgggtgt ccccatctac gccgagcgcg ggccgttggg
     1801 tggcgtgcag ctggtggagg gctaccgcac caggctcacg ggactgacgg ccgacgaagc
     1861 cgaagcgctg ttcctctccg gcctgacagg gccggcggcc gagctgggcc tgggcaccgt
     1921 ggtggcggca gcacagctca aggtccgagc ggcactgcca accgagctgc gctcgcgtgc
     1981 ctcgcgactg gtggagcgct tccacctcga cgccagcagc tggttccagc cgggagaggc
     2041 cgtggagcac ctgaccgtca tcgcccaggc cgtgtgggat acgcgcacgc tggaaatcgc
     2101 ctacgaccgg ggcgaccggc tggtggagcg caccgtcgaa cccttgggcc tcgtcctcaa
     2161 gggaggcacc tggtacctgg tcggacgcgt cgatgggcag ctgcggacgt atcgagcctc
     2221 gcggatcgtg tccgtcgagc ccacggatac cgtgttcgag cggccggctc atttcgacct
     2281 tgccgggtac tggcaggaga gcatctcagc ctacgagcgc gagacgcccc gcctggaaat
     2341 gaccattcgg gtcgcccccg gccgcacggg atccctcagc tgggtcctgg gggacgacgc
     2401 cctgtcgacg gccgaacgcg aaccgggacc cgatcccgaa ggatgggtcc ggcttcgcgt
     2461 caaggtcctg tggccggagg acgccgccgg acggctgctg tcgatgggtg gcgacctgga
     2521 agtggtcgat ccgccggagg tgcgggagga gctgctggcg ctggcccggt cagcgctgga
     2581 tcgccacgta tcgccgccgg ttgccggctg accgccggct cgtcagccct catgctcgtc
     2641 agccgtcctg gtggcgctga cgcgcgcgcg gctggcgcca tcggatgaac ggccgccgtc
     2701 cacccagcag gcgccgcacc cgcagcctgc tctcgacctc ccgctcgcgc tcctgctgga
     2761 tgacctgcat c
//
LOCUS       DATACA010000451         3818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2631318, whole genome shotgun sequence.
ACCESSION   DATACA010000451 DATACA010000000
VERSION     DATACA010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631318"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VHK28_09140"
     CDS             <1..129
                     /locus_tag="VHK28_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX2142417.1"
                     /translation="LLTQVTGIPCHVAENALNSVAVGTGLALEHFGTFRKSLVSTS"
     gene            126..1025
                     /locus_tag="VHK28_09145"
     CDS             126..1025
                     /locus_tag="VHK28_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2142418.1"
                     /translation="MTASAPTAAARAGAAATRERAGAPRQRAMAFADFHIHTRFSRDS
                     ILSEDKFIRIALERGLTHVAVTNHNNVEGAVAVRDRVAELGLDDRLTVILGEEVSTSD
                     GEVVGVFLSRTIPRGLTADQTADAIHEQGGLVSIPHPFDPFRGSHIRREPLERLAEAG
                     KIDMVEVFNSRVTFGRHNQEAAEFAARYGIPGIACSDSHSSFEIAMSFNALPAFATAD
                     ELREGLPENEWHGSRSTVLIHLTTRWAVWSNIVRRWLGKETAAAPVLGPEPPAKVEEE
                     PVKRPAPAELPDPEDPEATSDRG"
     gene            1111..2172
                     /locus_tag="VHK28_09150"
     CDS             1111..2172
                     /locus_tag="VHK28_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2142419.1"
                     /translation="MYEAREFLDAPAEEPIPEREVSLASRVLNWKTIASVVFAVVLLA
                     LAFRTLGANLADTWGLITQANLGLLLLAFAAYYATFPVRSFRWRYVLSKVGTRVGYRD
                     ATEILFLSWFVNCLVPAKLGDLYRAYLLKGTQGASASRTVGTIFIERIADIIVIFGLA
                     LAAGFWSFRNTERADMVDLLYLIGFVIAVVLVIIVLGLRMWGASLTARLPERFAALWE
                     RFHEGSTGALSLRALPVILTLTVVIWLLEGLRLYFVIRALSLPDVGLGISASVFVALA
                     AALLTAIPLTPAGVGFVEAGIAGALLIYGVQGDSAFAVALTDRGISILTVIVLGGLLY
                     LVSNKVRRAHGVGVPAGSG"
     gene            2525..2971
                     /locus_tag="VHK28_09155"
     CDS             2525..2971
                     /locus_tag="VHK28_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142420.1"
                     /translation="MTFQTPRSFNSWLRSQLKEKKMSQRQLALQSGVDHSTISRLIKG
                     DRMPSLGTATKLARGLREIREESEGPSYFASLSARQVLPTARVEYALRGDDSLTEADV
                     RELMQAYIALRSRRQRENGAAASAPNGEAHGADVDSRRVAGGVNRR"
     gene            complement(2958..>3818)
                     /locus_tag="VHK28_09160"
     CDS             complement(2958..>3818)
                     /locus_tag="VHK28_09160"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2142421.1"
                     /translation="QIFSDNPTTWRRRTDPPNDAADFVAYCRRHDIAPIAIHASYLVN
                     LAGTAEPFATQSRNMLIHELRRAPEYGAYLVNAHIGSHRGMGREAGLRRIVESVNAIL
                     AQTPEATTLVLENSAGGGDNLGSTIEELASMLEGVPSTSVHRLAFCLDTAHLWGAGHD
                     LSTAEGAQTVIDRFDRLIGLDRLKLVHLNDSRSELGSRTDRHEHVGGGRIGPAGLRAI
                     LTDPRLAERTAFMLETPGVDEGYDAVNLRRAWLLHQGVELPELPPRAFKLHRRSTRVA
                     PGSARKVSGG"
BASE COUNT          563 a         1349 c         1299 g          607 t
ORIGIN      
        1 ctgctgaccc aggtgaccgg catcccgtgc cacgtcgcgg agaacgcgct caacagcgtg
       61 gcggtgggca ccggcctggc gctggagcac ttcggcacgt tccgcaagag cctggtgagc
      121 acctcgtgac cgcctcggcg ccgacggccg ctgcccgagc cggagccgcc gcgacacgag
      181 agcgagccgg ggcgccgcgc cagcgtgcca tggccttcgc cgacttccac atccacaccc
      241 gcttcagccg cgactcgatc ctgtcagagg acaagttcat ccggatcgcc ctggagcgag
      301 gcctgaccca cgtggcggtc accaaccaca acaacgtgga gggtgcggtg gcggtgcgcg
      361 accgggtcgc cgagctgggg ctggacgatc gcctgaccgt gatcctgggc gaggaggtct
      421 cgacctccga cggcgaggtg gtcggcgtct tcctctcccg caccatcccc cgcgggctga
      481 ctgccgacca gacggccgat gccattcacg agcagggcgg cctggtctcc atcccgcacc
      541 cgttcgaccc gttccgcggc tcgcacattc gccgcgagcc gctcgagcga ctggccgagg
      601 cgggcaagat cgacatggtc gaggtcttca acagccgcgt caccttcgga cgccacaacc
      661 aggaggcggc ggagttcgcc gcccgctacg gcattccggg gatcgcctgc tcggactcgc
      721 attccagctt cgagatcgcc atgagcttca atgcgctgcc cgccttcgcc acggccgatg
      781 agctgcgcga gggcctgccg gagaacgagt ggcacggctc gcggtccacg gtgctcatcc
      841 acctgaccac gcgctgggcg gtgtggagca acatcgtccg ccgctggctg ggcaaggaga
      901 ccgccgccgc accggtcctc ggcccggagc cgccggccaa ggtcgaggaa gagccggtga
      961 agcggcccgc acccgccgag ctgcctgacc cggaggaccc ggaggcgacg agtgaccgag
     1021 gataagcggc cggaccctgc cggcagcggc ggggacgacc gacctcggga cacgcagcat
     1081 ccgtccgggc ctcacgagca cggtcactcg gtgtacgagg cccgtgagtt cctggacgcg
     1141 ccggccgagg agccgatccc cgaacgcgag gtgagcctcg ccagccgggt gctcaactgg
     1201 aagacgatcg cgtcggtcgt cttcgccgtg gtcctgctgg cccttgcctt ccggacgctg
     1261 ggagccaacc tggccgatac ctggggattg atcacgcagg ccaacctggg gctgctgctg
     1321 ctggccttcg ccgcctacta cgccaccttc ccggtgcgca gcttccgctg gcgttacgtc
     1381 ctgtcgaagg tcggcaccag ggtgggctac cgcgacgcga ccgagatcct gttcctgtcc
     1441 tggttcgtga actgcctggt gccggccaag ctgggcgacc tgtatcgggc ctacctgctg
     1501 aagggcaccc agggagcctc cgcctcgcgc acggtgggga ccatcttcat cgagcgcatc
     1561 gccgacatca tcgtcatctt cgggctggcg ctggccgctg gcttctggtc gttccgcaac
     1621 accgagcgcg ccgacatggt cgacctgctg tacctcatcg gcttcgtgat cgccgtcgtg
     1681 ctggtgatca tcgtccttgg cctgcggatg tggggcgcaa gcctcaccgc ccgcctgcca
     1741 gaacgcttcg cggcgctctg ggagcgcttc cacgagggga gcacgggggc cctctccctc
     1801 cgggcgctcc ccgtgatcct caccttgacc gtcgtgatct ggctgctgga gggtctgcgc
     1861 ttgtacttcg tgatccgcgc gctcagcctt cccgacgtgg ggctgggcat cagcgcgtcg
     1921 gtcttcgtgg cgttggcggc ggccctgctg accgccatcc cgctgacgcc ggccggcgtc
     1981 ggcttcgtgg aggccgggat cgccggcgcg ctgctgatct acggcgtgca gggcgactcc
     2041 gccttcgccg tcgcgctcac cgaccgcggc atcagcatcc tgaccgtgat cgtgctgggc
     2101 gggttgctgt acctggtgag caacaaggtg cgccgcgcgc acggcgtcgg ggtgccggcc
     2161 gggtcaggct agttggccgc cgatacatcg ggcgattctt cacgcaaggg gcctcctggg
     2221 tgtcaatccg ccgatatgtc gggcacctcc ccgcacgagc ggcacatagc ttcgccctcc
     2281 acgcgcacgc ctgcccgatg taacggcaca tcagcacgcc acagccctct cccgtgaagt
     2341 cctgcccgat agaacggcag cggacaaacc cagccagaca ccgccgagat ccccggtatc
     2401 cggccgatcc atcggtacct ttggcccatt gaggcacggc ccgacgcttg cgatgctccg
     2461 gttgctcgta cgcaccaccc cgggcaccat ccggggtgcc cgttcggcgc acggaccgag
     2521 gcgcatgacc ttccagacgc cgcgatcgtt caacagctgg ctgcggagcc agctgaagga
     2581 aaagaagatg agccagcgtc agctggcgct gcagtcgggc gtggatcact ccacgatctc
     2641 gcggctcatc aagggcgacc gcatgccgtc gctgggcacc gcgacgaagc tagcccgtgg
     2701 gctgcgagag attcgcgagg agtcggaggg tccctcctac ttcgcgtcgc tctccgcccg
     2761 tcaggttctg ccaaccgctc gagtcgagta cgcactgcgc ggagacgact ccctcactga
     2821 agccgatgtt cgcgagttga tgcaggcata catcgcattg cgtagccgac gtcagcggga
     2881 gaacggcgcg gccgcttcag cgcccaatgg ggaagcccac ggcgccgacg tggacagccg
     2941 gagggtggcc gggggcgtca accgccgctg accttgcgcg cggaacccgg cgcaacccgg
     3001 gtggatcgcc ggtgaagctt gaaggcccgc ggcggcagct ccggcagctc cacaccctgg
     3061 tgcaggagcc aggcccggcg caggttgacc gcgtcatagc cctcatcgac ccccggcgtc
     3121 tccagcatga acgccgtgcg ctccgccagc cgcgggtcgg tcaggatggc tcgcagccca
     3181 gctggaccga tgcgtccgcc gcccacgtgc tcgtgccggt ccgtccgcga cccgagctcc
     3241 gaccgcgagt cgttgaggtg caccagcttc agccggtcga ggccgatcag ccggtcgaac
     3301 cggtcaatca ccgtttgggc cccctctgct gtggagaggt cgtggcccgc gccccacagg
     3361 tgcgcggtat ccaggcagaa cgccagccgg tggaccgatg tcgacggcac tccctccagc
     3421 atgctggcca gctcctcgat cgttgagccg aggttgtcgc cgccaccggc ggagttctcc
     3481 aggaccaggg tcgtggcctc cggcgtctgg gccaggatgg cgttgacgct ctccacgatc
     3541 cgtcggagcc ccgcctcgcg acccatgccg cggtgcgaac cgatgtgggc gttgaccagg
     3601 tacgcgccgt actccggcgc gcggcgcagc tcgtggatga gcatgttgcg cgactgggtg
     3661 gcgaagggct ccgccgtgcc ggccaggttg accaggtagc tggcgtggat ggcgatcggc
     3721 gcgatgtcgt gccggcggca gtaggccacg aagtctgcgg cgtcgtttgg cggatcggtg
     3781 cgtcggcgcc aggtggtcgg gttgtcgctg aagatctg
//
LOCUS       DATACA010000452         3847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1829210, whole genome shotgun sequence.
ACCESSION   DATACA010000452 DATACA010000000
VERSION     DATACA010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829210"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..868
                     /locus_tag="VHK28_09165"
     CDS             212..868
                     /locus_tag="VHK28_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142422.1"
                     /translation="MALHTPVYRLVGRRARLERIATALGDPLIEELPIPLAISSYDLT
                     TGQATAIVSGRLVDAVERSIAVPLFFPPCRHGDGVWCDAGPWESVPVSLARRLSPEPV
                     IGSWVDIPKPGFLTRRPVAALLHRAALQLGRAASPGRLTARSYLALLAERYAEPVHFE
                     EPELLVRPRLGLTPAWQFTRVRHMAAIGYRDTQLALADAGLAGPAEASVQDRGERRAA
                     "
     gene            858..1787
                     /locus_tag="VHK28_09170"
     CDS             858..1787
                     /locus_tag="VHK28_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2142423.1"
                     /translation="MPPDRPGLLLVHAHPDDECFATGGLIARAVAEGHRVDLVTCTGG
                     EEGEIHDPSLDYEEAFPRLAQIRAEELRCSLEALRHGGPPGSAPGELHLHMLGYRDSG
                     MMGTPSNERPEAFWNADLEEATRRVVAIVREARPAVMVTYDQNGNYGHPDHINAHRVA
                     AAAWDGAADVGRFPDGGPSHEVAKFYEVAFNRDRWTTLMTEMKERGIDLPWDFGESDS
                     EPPEGAAETAEEEFGVPEADITTIVDVSGYARAKRTSMDCHKTQRQDMGWLLDLPDDL
                     AAKALSPEHFMLTRWRGRDVPSGLREASVFDGL"
     gene            complement(2017..2673)
                     /locus_tag="VHK28_09175"
     CDS             complement(2017..2673)
                     /locus_tag="VHK28_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2142424.1"
                     /translation="MASRVSWVSEERLRARRVRVEIGHEIRNARLAKGLRQLDVARAA
                     NFSRSKMGRIERGESRKVTHEDLVLLAAAVGLRLSIQLYPAVSRLRDGRQAGMITRYL
                     GLIGDGGWAANTEVPVGRRGDLRAIDLVLRRDGIRIAHEFVSALRDAQAQSRPLQLKA
                     VDAGIQRLILVIAATHANRQAVAEAGAALRASFPLGTREVLAALRSGRDPGANGIVFL
                     "
     gene            2933..3601
                     /locus_tag="VHK28_09180"
     CDS             2933..3601
                     /locus_tag="VHK28_09180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2142425.1"
                     /translation="MSNRSIGLDENLHAYLLKVGVREHPVLAALRDETARMPNHNMQI
                     APEQGALLALLVRLGNVRRIVEIGTFTGYSSTAMALALPPDGRIVCCDVNREWTDVAR
                     GAWTDAGVADRVELRLGPALETLDQLLAEGGENSFDMAFIDANKSDYDGYYEAALRLL
                     RPGGLVAIDNVLWSGRVADPSDRDEDTDAIRALNAKIAADERVELGMTPVGDGLTLAR
                     LRDA"
     gene            3617..>3847
                     /locus_tag="VHK28_09185"
     CDS             3617..>3847
                     /locus_tag="VHK28_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX2142426.1"
                     /translation="MPPAHLIEAAERWGTPLYVTDLDAAAAAYATYARAFPGALIAYA
                     VKSNPDPRLLARLAAEGAGAEVVTAVELALATR"
BASE COUNT          594 a         1435 c         1274 g          544 t
ORIGIN      
        1 catgacacgg ggctgttcac ctactcgaag ctgcgcgaga cgtgcacgtg caacgagcat
       61 cgagcggccg cggccgcctc gtgagcccgc cgccgcttgc cattcgcgac cgggcgtaca
      121 gcctggcgct gggcggtggc ggtggccgcg gctgggcgca tatcggcgtt gcgcgggcgc
      181 tggacgaggc gggcctgcgt ccggcgagaa ggtggccctg cacacccccg tctaccgcct
      241 ggtcggccgc cgggcgcgcc tggagcgcat tgccacggcg ctcggggacc cgctcatcga
      301 agagctgccg atcccgctgg ccatcagcag ctacgacctg accaccggcc aggcgacagc
      361 catcgtctct ggccgcctgg tggacgccgt ggagcgcagc atcgccgtgc cgctcttctt
      421 cccgccctgc cgccacggcg acggcgtgtg gtgcgacgcc ggtccgtggg agtccgtgcc
      481 ggtcagcctg gcgcgtcgcc tctccccgga gccggtcatc ggttcgtggg tggacatccc
      541 caagccggga ttcctgaccc ggcggccggt cgccgccctc ctccaccgtg cggcgctgca
      601 gctgggtcgc gccgccagtc cgggacgcct caccgcccgc agctacctgg ccctgctcgc
      661 cgagcgctac gcggaaccgg tccacttcga ggagccggag ctcctcgtcc gcccgcgcct
      721 ggggctgacg ccggcctggc agttcacgcg agtgcggcac atggccgcca tcgggtaccg
      781 ggacacgcag ttggcgctcg ccgacgccgg gctggcggga ccggcggagg catcggtcca
      841 ggacagggga gagcggcgtg ccgcctgacc ggccggggct gctgctggtc cacgctcacc
      901 cggacgacga gtgcttcgcc accggcgggc tgatcgcgcg ggccgtggcg gagggacacc
      961 gcgtcgacct ggtgacctgc accggtggcg aggagggcga gatccacgac ccctcgctcg
     1021 actacgagga ggccttcccc cgcctggcac agatccgcgc cgaggagctg cgctgctcgc
     1081 tcgaggcgct gcggcacggc ggcccgcccg gctctgcacc cggcgagctg cacctgcaca
     1141 tgctcggcta tcgggactcg gggatgatgg gcacgccatc caacgagcgg cccgaggcat
     1201 tctggaacgc tgacctggag gaggccacgc ggcgggtggt ggccatcgtt cgcgaggcgc
     1261 gtccggcggt gatggtcacc tacgaccaga acggcaacta tggccaccct gaccacatca
     1321 acgcccaccg ggtagcggcc gccgcctggg acggggcggc ggatgttggc cgcttcccgg
     1381 acgggggtcc atcgcacgag gtcgccaagt tctacgaggt cgccttcaac cgggaccgat
     1441 ggaccacgct catgaccgag atgaaggagc gcggcatcga cctcccgtgg gacttcggcg
     1501 agtccgacag cgagccgccc gagggcgcag ccgagaccgc ggaggaggag tttggcgtcc
     1561 cggaggccga tatcacgacc atcgtcgacg tctccggcta cgcccgcgcc aagcgcacgt
     1621 ccatggactg ccacaagacc cagcgccagg acatgggctg gctcctggac ctgcccgatg
     1681 acctggccgc caaggccctc tcccccgagc acttcatgct cacccgatgg cgcgggcgcg
     1741 acgtgccgtc tggcctccgc gaggcatcgg tcttcgacgg tctctagccc gccaggctgg
     1801 gctgcagtca actcgacacc gcggtgctca ctggttgggc actacagcca agcagccact
     1861 tagaacggcc cccggctggc tgccacgctc acccgacgga ccaaacggcc atctgtatgc
     1921 tcgcttcagc gtttcattgg ctgttatgcc catctcacgg ccacggctac agtcacgagc
     1981 ggcccggcac agcgggacag ccaacatggc cacatatcag aggaatacga tcccattcgc
     2041 accgggatca cgcccggatc gcagggccgc gagcacctct cgcgtcccca gtgggaacga
     2101 cgcccgaaga gccgcgcccg cctcggccac cgcctgtcgg ttggcgtggg tagccgcgat
     2161 caccaggatc agccgctgga ttccggcgtc gaccgccttg agctgcaggg gtcgtgactg
     2221 cgcttgggca tcccgcaacg cgctgacgaa ttcatgcgcg atgcgtatcc catcccttcg
     2281 cagaacgagg tcaatggccc gcaggtctcc ccgccgaccg acgggcacct ccgtattagc
     2341 tgcccatccc ccgtccccga tcagccccag gtaccgcgtg atcatgccgg cctgccgccc
     2401 gtcccgcagg cggctgacag ccgggtagag ctggatgctc aggcgaagac caacagccgc
     2461 ggccagaagt acgaggtcct cgtgcgtcac cttgcgcgac tcgccgcgct cgatgcggcc
     2521 catcttcgaa cgggagaagt tggcggcacg cgccacatcc aattggcgca ggcccttcgc
     2581 caggcgcgcg ttccgaatct catggccgat ctcgacgcga acccgccttg cccgcagccg
     2641 ctcctcgctg acccagctga cccgactggc catggcggca tggttccgca cgaccgtgta
     2701 ccgccggctt atcgcaggct taccaggtcg tgctcatgga acgggcacta cggccaagca
     2761 gccactcaga acggccgccg gctggctgcc acgctcaccc gacggaccaa gcggccatct
     2821 gtatacccgc ttcagcgtct ccttggccgt tatacccacc tcacgggcac ggccacagcc
     2881 acggccacgg ccacgctcag ccagacactc ggtcggctac catcggtcca gcatgtccaa
     2941 ccgctccatc ggcctcgacg agaacctcca cgcctacctg ctgaaagtcg gcgtccgcga
     3001 gcatccagtg ctcgccgccc tccgcgacga gacggcgcgg atgcccaacc acaacatgca
     3061 gatcgccccg gagcaggggg ccctgctcgc tctcctggtg cgcctgggca acgttcgcag
     3121 gatcgtggag atcggcacct tcaccggcta ctcctcgacc gccatggcgc tggcactgcc
     3181 cccagatggc cggatcgtgt gctgcgacgt gaaccgcgaa tggaccgatg tcgcccgcgg
     3241 cgcctggacc gatgccggcg tcgccgaccg cgtcgagctc cgcctgggtc ccgcgctcga
     3301 gacgctcgac cagctgctgg ccgagggcgg agagaacagc ttcgacatgg cgttcatcga
     3361 tgccaacaag tcggactacg acggctacta cgaggcggcg ctccggctgc tgcgacccgg
     3421 cgggctggtc gccatcgaca acgtgctgtg gagcggacgg gtggccgatc catcggaccg
     3481 cgacgaggat acggacgcca tccgcgcgct caacgccaag atcgccgccg acgagcgcgt
     3541 ggagctcggc atgacgccgg tgggggacgg gctgacgctc gcacgactgc gcgacgcgta
     3601 gccccacccg gtggccgtgc cccccgcaca cctgatcgag gcagccgagc gctggggaac
     3661 gccgctctac gtcaccgacc tcgacgccgc cgcagccgcc tacgccacct acgcgcgagc
     3721 cttccccggc gcgctgatcg cgtacgcggt caagtccaat ccggacccgc ggctcctcgc
     3781 acggctggcc gccgagggcg ccggggcaga ggtggtcacc gctgtcgagc tggccctggc
     3841 cacgcgc
//
LOCUS       DATACA010000453         4296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1667828, whole genome shotgun sequence.
ACCESSION   DATACA010000453 DATACA010000000
VERSION     DATACA010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667828"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VHK28_09190"
     CDS             <1..189
                     /locus_tag="VHK28_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142427.1"
                     /translation="GAVQGYVTVCADRCARLPIVDWCQCYWGTADKRVADLSHAAWPL
                     VTDQPRSRGLVPVRVFLE"
     gene            complement(205..663)
                     /locus_tag="VHK28_09195"
     CDS             complement(205..663)
                     /locus_tag="VHK28_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142428.1"
                     /translation="MTPDTRPARACIKCGREIGPDESICEVCNRAGMATPSASQYHGT
                     MAVAIIAGVVALAIWASLAMRGVGPYAAEVLDMVADPPDGATVTFRVENEGTSRGFAN
                     CQLVVRDAGGHQMRSRSVTAGPLDGGADGTFTERVGGLPSDPASASVECT"
     gene            complement(660..1286)
                     /locus_tag="VHK28_09200"
     CDS             complement(660..1286)
                     /locus_tag="VHK28_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX2142429.1"
                     /translation="MSEYLDLADWRRTVAALYAAMRRDRRAAPLRLMRFRAAKDRLFA
                     EHSSSPIPAGARSRFRGLAYWRHDPALRLAAGLVPDPAAAPLDVPRSGEGAGMPFSRI
                     GWVEFTASGSACRLAVYWLSEYAGGIFIPFRDATSGRETYGGGRYLWDSAKGADLGSD
                     GEQLVLDFNYAYHPSCVYDPAWSCPLAPQENWLPVAIEAGERLEPVSS"
     gene            complement(1369..2337)
                     /locus_tag="VHK28_09205"
     CDS             complement(1369..2337)
                     /locus_tag="VHK28_09205"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2142430.1"
                     /translation="MRINRMLALGAVMLALLVGACTPGGGGTTSPGTSPDADSPGNSP
                     SMGAELPTVVIGSQGFYESQLMAEIYAQALEAAGYSVERNLGIGTREQTMPAILDGQI
                     DLIPEYIGSLLRGGLAGEPTGDPEETAELLAEAAAEEGLSVLDYTPAQDTNAFVVRQE
                     TADELGLETMSDLAEVATDMVWGLPPECETNPLCAGALEDAYGIPFAELEIEPLAACN
                     APIATALNDGVVDVAELCSTQPAIEQFNFVVLTDDQMTQPAENIAPLVRTEFLDAAPE
                     SFAETLNAVSAEMTTEDLTQLGVEVAVQNRDVAEVAAEWLTEMGLV"
     gene            complement(2485..3219)
                     /locus_tag="VHK28_09210"
     CDS             complement(2485..3219)
                     /locus_tag="VHK28_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142431.1"
                     /translation="MEFLSDVLAWVSDPANWWGADGIPVRIGEHLVLSAVPLLVAAGV
                     ALPIGLYIGHTGRLAGVAINASNVGRAVPTLAILVIAWILVSRPLVDLGLRREAAEVT
                     TAIAMLALALPPIVTNTYVGIAQVDRDLVEAARGVGMGPMQVLRRVELPLALPVVLAG
                     LRTAAVQVVATATLGAVLGTGGLGRYLIDGIAQRRYEEMFAGAVLVSLLAIGTELAFA
                     WLQRRTVSPGLRGTAPGAAPSMPQPA"
     gene            complement(3226..3897)
                     /locus_tag="VHK28_09215"
     CDS             complement(3226..3897)
                     /locus_tag="VHK28_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142432.1"
                     /translation="MSEGQPVIDVGWIVRNLGDIGQRTLDHVVLTVLAVGIGLLISFG
                     LALLVRRWRPLHGPVLAVTGTLYSIPSLALFSLLIPFTGIQSILTAEIALVSYTLLIL
                     VRNIVTGLDSVPPDVLEAAHGMGYTPWQRLLRIELPIALPVILAGVRIATVTTVGLVT
                     VTALIGIQSLGSMIYNIGLQRFFLTATLVGSLGAVALAVIADLGISALQRVLTPWSRS
                     RASGT"
     gene            complement(3894..>4296)
                     /locus_tag="VHK28_09220"
     CDS             complement(3894..>4296)
                     /locus_tag="VHK28_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2142433.1"
                     /translation="DRGLKRLSLARVRDLPLLEPMTVRVGEVAADARRRLEEADVDYA
                     LLVDAARRPLGWIDPGDLAGDGEIGPEAATPGAPTVQPETTLRDALSGMLASAVQLGV
                     VVDEREAVLGLISVNAISETLRAATDADGGG"
BASE COUNT          621 a         1548 c         1535 g          592 t
ORIGIN      
        1 ggcgccgtgc agggatacgt caccgtctgc gccgatcgct gcgcgcgact gccgatcgtg
       61 gactggtgcc agtgctattg gggcaccgcg gacaagcggg tggcggacct gtcgcacgcc
      121 gcctggccgc tggtcaccga ccagccgcgc tcacggggcc tggttccggt gcgcgtcttc
      181 ctggagtgac cggccggcac ggcctcaggt gcactccacc gaggccgatg cagggtctga
      241 cggcagcccg cccacgcgct cggtgaacgt cccatcggcg ccgccgtcca gcggcccggc
      301 ggtcaccgag cgtgagcgca tctggtgccc gcccgcatcg cgaacgacca actggcagtt
      361 ggcgaagccg cggctggtgc cctcgttctc gacccggaag gtgacggtgg cgccgtccgg
      421 cgggtcggcc accatgtcca ggacctcggc ggcgtaggga cccacgccgc gcatcgccag
      481 gctggcccag atggccagcg ccaccacacc ggcgatgatg gcgaccgcca tggtaccgtg
      541 gtactgcgat gcggacggcg tcgccatgcc ggcccggttg cagacctcgc agatggactc
      601 gtcggggccg atctcgcggc cgcacttgat gcaggcgcgt gccggtcgtg tgtcgggggt
      661 catgaggaca ccggctccag tcgctcgccg gcctcgatcg cgaccggcaa ccagttctcc
      721 tgcggcgcga gcgggcagct ccaggccggg tcgtacacgc aggatgggtg gtaggcgtag
      781 ttgaagtcga gcaccagctg ctcgccgtcc gagccgaggt ccgccccctt ggccgagtcc
      841 cacaggtagc ggccgccgcc gtaggtctct cgtccgcttg tcgcgtcgcg gaaggggatg
      901 aagatgccgc ctgcgtactc gctcagccag tagaccgcca gccggcaggc gctcccggat
      961 gccgtgaact cgacccaacc gatgcggctg aacggcattc cggccccttc gccagagcgc
     1021 ggtacgtcca gcggcgcggc agccgggtcg gggaccaggc cggcggccag gcgcagcgcg
     1081 gggtcgtgac gccagtaggc caggccgcgg aagcgggaac gcgcgccggc cgggatcggc
     1141 gaggaggaat gctcggcgaa aagcctgtcc ttggcggccc ggaagcgcat cagccgcagt
     1201 ggcgccgccc ggcggtcgcg gcgcatggcg gcgtagaggg ccgccaccgt gcggcgccaa
     1261 tcggcgaggt cgaggtactc ggacatgcgt ctggggcaag gatagaagcg ggccgcctcg
     1321 tgcgccccgc ccctatcagt ctcgatcggc tccggctcga ggcgatgcct agacgagccc
     1381 catctcggtg agccactcgg cggccacctc ggccacgtcg cggttctgga cggccacctc
     1441 cacgcccagc tgggtgaggt cctcggtagt catctcggcg ctcaccgcat tcagggtctc
     1501 ggcgaagctc tccggcgccg cgtccaggaa ctcggtgcgc accagcgggg cgatgttctc
     1561 ggccggctgg gtcatctgat cgtcggtgag caccacgaag ttgaactgct cgatggccgg
     1621 ctgggtgctg cacagctcgg ccacgtccac cacgccgtcg ttcagcgcgg tggcgatcgg
     1681 cgcgttacac gcggccagcg gctcgatctc cagctcggcg aacgggatcc cgtaggcgtc
     1741 ctccagggcc ccggcgcaca gcggattggt ctcgcactcc ggcggcagtc cccacaccat
     1801 gtcggtggct acctcggcca ggtcgctcat ggtctcgagg ccgagctcgt cggccgtctc
     1861 ctggcgcacc acgaacgcgt tggtgtcctg cgccggcgtg tagtccagca cgctcaggcc
     1921 ctcctcggcc gcggcctccg ccagcagctc cgcggtctcc tccggatcgc cggtcggctc
     1981 gccggccagg ccaccgcgca ggaggctgcc gatgtactcc gggatgaggt cgatctgccc
     2041 gtccaggatg gccggcatgg tctgctcgcg ggtgccgatg cccaggttgc gctccacgct
     2101 gtagccggct gcctcgagcg cctgggcgta gatctcggcc atcagctgcg actcgtagaa
     2161 gccctgggaa ccgatgacca cggtcggcag ctcggcgccc atgctcgggc tgttgcccgg
     2221 gctgtccgcg tccgggctgg tgcccgggct cgtggtgccg cccccacccg gcgtgcaggc
     2281 gccgaccagc agcgccagca tcaccgcccc gagggcgagc atgcggttta tccgcattgg
     2341 gtcctccttg gtgctacgtg tacttaccag taaccatacc cacgccggta acgcaccgtt
     2401 cagcgcgcgg ctcgacaggg ggtggcgaga gcgagtctaa cagcgtcgct gcgaagggcc
     2461 gatgacgggg acctgcggct cgcttcaggc cggctgtggc atggatggcg cggcgccggg
     2521 ggccgtgccc cgcaggccgg gcgacacggt tcgccgctgg agccaggcga aggcgagctc
     2581 ggtgccgatg gccagcagcg agacgaggac ggcccccgcg aacatctcct cgtagcgccg
     2641 ctgggctatg ccgtcgatca ggtagcggcc caggccgccg gtgcccagca cggcgcccag
     2701 cgtggcggtg gccacaacct gcaccgccgc ggtccgcagc ccggccagca ccaccggcag
     2761 cgccagcggc agctctacgc ggcgcagcac ctgcatgggt cccatgccga cgccgcgcgc
     2821 ggcctccacc aggtcgcggt ccacctgggc gatgcccacg taggtgttgg tcacgatggg
     2881 cggcagggcc agcgccagca tggcgatggc ggtcgtcacc tcggccgcct cgcggcgcag
     2941 gccgaggtcg accagcgggc ggctgaccag gatccaggcg atcaccagga tggccagggt
     3001 cgggaccgcg cggcccacgt tggaggcgtt gatggccacc ccggccagcc ggccggtgtg
     3061 cccgatgtac aggccgatgg gcagcgccac gccggcggcc accagcagcg gaacggccga
     3121 caggacgagg tgctcgccga tgcgcaccgg gatgccatcc gccccccacc agttggcggg
     3181 gtccgatacc caggcgagga cgtcggacag gaactccatc ggtctctacg tcccggacgc
     3241 gcgcgagcgt gaccaggggg tcagcacccg ctggagggcg ctgatgccca ggtcggcgat
     3301 gaccgccagc gccaccgcac cgagtgaccc gaccagcgtc gcggtcagga agaagcgctg
     3361 cagcccgatg ttgtagatca tgctgccaag gctctggatc ccgatcagcg cggtcacggt
     3421 caccaggccc accgtggtaa cggtggcgat gcgcaccccg gccaggatga cgggcagggc
     3481 gatcggcagc tcgatgcgca gcagccgctg ccagggggtg tagcccatgc catgggccgc
     3541 ctccagcaca tcgggcggca cactgtccag gccggtcacg atgttgcgca ccaggatcag
     3601 cagcgtgtag ctgaccaggg cgatctcggc ggtcaggatg ctctggatgc cggtgaacgg
     3661 gatcagcagg ctgaagagcg ccaggctcgg gatcgagtac agcgttccgg tcaccgccag
     3721 caccggcccg tgcaggggtc gccagcggcg gaccagcagc gcgagcccga agctgatgag
     3781 cagcccgatg ccgaccgcca gcacggtgag caccacgtgg tccagcgtgc gctggccaat
     3841 gtcgcccagg ttgcggacga tccatccgac gtcgatgacc ggctgcccct cgctcatccg
     3901 ccgccgtccg cgtccgtcgc ggcgcgcagg gtctcgctga tggcattcac gctgatcagg
     3961 cccagcaccg cctcgcgctc gtccaccact acgccgagct ggacggccga ggcgagcatg
     4021 cccgacagcg cgtcgcgcaa cgtcgtctca ggctgcacgg tcggcgcccc gggggtggcc
     4081 gcctccgggc cgatctcgcc gtcgccggcc aggtcgccgg ggtcgatcca gcccagcgga
     4141 cgcctggcgg cgtccaccag cagggcgtaa tcgacgtccg cctcctccag gcgccggcgc
     4201 gcatcggctg ccacttcgcc gacccgcacc gtcatcggct ccaggagcgg aaggtcgcgc
     4261 acccgcgcca gcgagaggcg cttcaggccg cggtcc
//
LOCUS       DATACA010000454         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1747895, whole genome shotgun sequence.
ACCESSION   DATACA010000454 DATACA010000000
VERSION     DATACA010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747895"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VHK28_09225"
     CDS             <1..307
                     /locus_tag="VHK28_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142434.1"
                     /translation="TVRRVIDQAAAAGRTVIAISHDMEFVAEAFARVVVLREGHLILD
                     GPTASVFAPDRWDSLASTYLEAPLAARLGARLGLNHVTPTDAALLAALGAAGPAGDQ"
     gene            complement(301..756)
                     /locus_tag="VHK28_09230"
     CDS             complement(301..756)
                     /locus_tag="VHK28_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142435.1"
                     /translation="MTSPAPMARDARVREALYRTRVLANRTPHFNTRTRREANRGLDM
                     LDGQLGRNQVNTAEAMRGIELLNRAHSSLLFGLLRDPSFADRFGPALRRLGLRGIEQR
                     LNEVPSSVMALPIPGPTGERRHRDELPPDERTDAEGNLLPPPPGFYTGY"
     gene            755..1450
                     /locus_tag="VHK28_09235"
     CDS             755..1450
                     /locus_tag="VHK28_09235"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2142436.1"
                     /translation="MERILRAEGDWQATLAKALRRVPRPIPVDPRLTPRDREATGSPR
                     YERSSMPPARRAATLMALYPRLVEGALQLVIPLTVRHAGLRSHAGEVSLPGGAVDETD
                     ASPQAAALREAWEEVGMAPDEVEILGSLDSVWIPVSNFELRAFVGVLSTAPTLVAHEP
                     EVEAIVELPARVLVADDGLIEELIEGPGWRLRAGGYRHGEHLVWGATARSLAMFGSVL
                     RLAAELSGESSAG"
     gene            complement(1474..2298)
                     /locus_tag="VHK28_09240"
     CDS             complement(1474..2298)
                     /locus_tag="VHK28_09240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX2142437.1"
                     /translation="MSGWQDELDFSVEVARRAGELLKASYGRVERIDRKSKRDVVTEV
                     DFASEQLVLDAIRETHPGDEILAEESGHHERHGPQATPTGRTWVIDPLDGTVNYANAI
                     PYFCVSVALVDGDRPAVGVVFDPMRGDCYAATADGQAVLNEVRIHASDKDQLGDFVVS
                     LAVIGRGGIGRERRISREIRIPRRMGSAALSLAYVASGRFDAFVQNGGLSVWDVAAAG
                     LIAERAGATVSNLEGGNWWDASRRSSTVSIVAAPQPHHGDLLSLLASVGTRVRTRR"
     gene            2406..3167
                     /locus_tag="VHK28_09245"
     CDS             2406..3167
                     /locus_tag="VHK28_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2142438.1"
                     /translation="MGQGAKRVRVRRRPRPLKWWRQPLALKPARRGTLADRAADAIRR
                     RIVLGRVKPGQRLEAMRELARQLNVSLGVVREAVAQLKAEGVVQVHHGRGTYVARRPA
                     AARVLRAARRRAGRRELEELRQAVEPALAHAAAKRVRARPRDLTELHLAMLERTHARS
                     WATSEAYTKADLEVHAAVARLSGNALGAAAYRMAAVGLRPHLVSRARDLAKDDRLHRM
                     HEALVDAIEHGRPVRARRAAAAIAAIEGGSRAPPA"
     gene            3182..>3511
                     /locus_tag="VHK28_09250"
     CDS             3182..>3511
                     /locus_tag="VHK28_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX2142439.1"
                     /translation="MPQIHLHAEPGDYAPLVLLPGDPNRARRIAERFDGGLEAARQVN
                     THRGLLGWTGTVEGRPVSVQATGMGTPSLSIVVEELLRLGARQLIRVGTCGGIGPGLR
                     TGDLVVAV"
BASE COUNT          502 a         1240 c         1244 g          525 t
ORIGIN      
        1 caccgtgcgc cgggtgatcg accaggcggc cgcggccggc cggacggtca tcgccatcag
       61 ccacgacatg gagttcgtgg ccgaggcgtt cgcccgcgtc gtcgtcctgc gcgagggtca
      121 cctcatcctc gacgggccga ccgcatcggt ctttgcgccg gaccgatggg attcactggc
      181 ctcgacctac ctcgaggcac cgctagccgc ccgcctgggt gcgcgcctgg ggctcaacca
      241 cgtcacgccg accgatgccg ccctgctcgc cgcgctcggc gcagctggcc cagcgggcga
      301 tcagtagccg gtgtagaacc ccggaggggg tggaaggagg ttgccctcgg catcggtccg
      361 ctcgtcgggc ggaagctcgt cgcgatgccg ccgctccccc gtcggccccg ggatgggcag
      421 ggccatgacc gagctgggga cctcgttcag ccgctgctcg atgccgcgca gccccaggcg
      481 ccgcagcgcc ggcccgaatc ggtccgcgaa ggagggatcg cgcagcaggc cgaacagcag
      541 cgagctgtgc gcgcggttca gcagctcgat gccgcgcatc gcctcggcgg tgttcacctg
      601 gttgcggccc agctggccgt cgagcatgtc cagccctcga tttgcctcgc ggcgtgtccg
      661 cgtgttgaag tgcggcgtgc ggttggcgag cacccgcgtt cggtagagag cctcgcgtac
      721 ccgcgcatcg cgggccatcg gtgcagggga ggtcatggag cgcatcttac gcgctgaggg
      781 cgactggcag gcaaccctgg ccaaggcgtt gcgacgcgtc ccgcggccga tcccggtgga
      841 cccccgcctg accccccgcg accgcgaagc cacgggttca ccccgctacg aacgatcgtc
      901 gatgccaccc gcccggcgcg ccgccaccct gatggcgctc tacccccgcc tcgtggaggg
      961 cgcgctccaa ctggtgattc cactcaccgt gcgccacgcc ggcctgcgct cacatgccgg
     1021 tgaggtgtcg ctcccgggtg gcgcggtgga cgagaccgat gcctctcctc aggccgccgc
     1081 cctgcgcgag gcctgggagg aggtgggcat ggcgcctgat gaagtcgaga tcctcggttc
     1141 gctggactcg gtctggatcc cggtcagcaa cttcgagctg cgggccttcg tcggcgtgct
     1201 gtccacggcg cccaccctgg tcgcccacga gccggaggtc gaggccatcg tcgagttgcc
     1261 ggcaagggtg ctggttgccg atgacggact gatcgaggag cttatcgagg gtcccggctg
     1321 gcggctgcgg gcgggtggct accgccacgg agagcacctc gtgtggggcg caaccgcacg
     1381 cagcctggcc atgttcggtt ccgtgctgcg cttggcggcg gagctgagcg gcgaatcgtc
     1441 ggccggttga cgccgtccaa atgcgcccgc cgcctaacgg cgggtccgga cccgggtgcc
     1501 gaccgaagca agcaggctca gcaggtcccc atggtggggc tgcggggcgg ccacgatgct
     1561 caccgtgctg ctccggcggc tcgcgtccca ccagtttccg ccctccaggt tgctcaccgt
     1621 cgccccggct cgctcggcga tcaggccggc cgccgcgacg tcccacactg aaagcccgcc
     1681 gttctgcacg aacgcatcga acctgccact cgccacgtag gccaacgaca gcgctgcgct
     1741 tcccatcctc cgcgggatcc ggatctcgcg cgagatccgt cgctcgcgcc ctatcccccc
     1801 acgcccgatg actgccaggc tgaccacgaa gtcccccagc tggtccttgt ccgaggcatg
     1861 gatccgaacc tcgttcagga ccgcctggcc gtccgcagtc gccgcgtagc agtctccacg
     1921 catcgggtcg aacactaccc cgaccgccgg ccggtccccg tccacgagcg ccacgctcac
     1981 gcagaaatag ggaatggcat tcgcatagtt caccgtcccg tccagcggat cgatcaccca
     2041 ggtacgcccc gtcggcgtgg cctgcggccc atggcgctcg tgatgacctg attcttcggc
     2101 gaggatctcg tcgccgggat gcgtctcgcg gatggcatcc aggaccagtt gctcggaggc
     2161 gaagtccacc tcggtgacca catcgcgctt gctcttccga tcgatacgct ccacccgtcc
     2221 atagctggcc ttgagcagtt cgcccgctcg ccgcgccacc tccaccgaga agtcgagctc
     2281 atcctgccaa ccactcatcc gctcgatact acttggtgcc cgcccgcagt aagcctccgg
     2341 taaccacgtg gtaacgcccc atctgcaagt gttccggtga tctgaacact ttggaggcat
     2401 ggggcgtggg gcaaggggca aagcgggttc gggttaggcg gcggccgagg ccgctgaaat
     2461 ggtggcggca gccgctggcg ctcaagccag cgcgacgagg aacgctggct gatcgcgcgg
     2521 cagacgccat tcgtcgccgg atcgtgctcg gcagggtgaa gccggggcag cggctggaag
     2581 ccatgcgcga gcttgcgcgg cagctgaatg tctccctcgg ggtcgtcaga gaggcggttg
     2641 cgcagctgaa ggccgaaggg gtggtgcagg tgcaccatgg ccggggcacc tatgtcgccc
     2701 gtcgaccggc tgccgcgcgg gtgctgcggg cggcgaggcg aagggctggg cggcgcgagc
     2761 tcgaggagct gcggcaggcg gttgagcccg cgctggcgca cgccgccgcc aagcgcgtgc
     2821 gggccaggcc gcgggacctg accgaactgc acctggccat gctggagcgg acgcacgcgc
     2881 ggtcgtgggc cactagcgag gcgtacacca aagcggatct ggaggtccat gccgccgtgg
     2941 cacggctgag cggcaacgcg ctcggggcgg cggcgtacag gatggcggcc gtcggcttac
     3001 gcccgcacct cgtgtcgcga gcccgcgacc tcgccaagga cgaccggttg caccggatgc
     3061 acgaggcctt ggtcgacgcg atcgagcacg gacggccagt tagggcgcgg cgcgcagcgg
     3121 cggccatcgc ggcaatcgag ggtggctccc gggcgccacc agcctgacgt accctagcga
     3181 catgccacag atccacctcc acgccgagcc gggcgactac gccccgctgg tcctgctgcc
     3241 cggggatccg aatcgggcgc ggcgcatcgc ggagcggttc gacggcgggc tggaggcggc
     3301 tcgccaggtg aacacgcaca gggggttgct cggctggacg gggaccgtcg agggccgtcc
     3361 ggtcagcgtt caggccacgg ggatgggcac gccgagcctg tcgatcgtgg tggaggagct
     3421 gctgcggctc ggtgccaggc agctcatccg ggttgggacc tgcggcggca tcggtcccgg
     3481 actgcgcacc ggagacctgg tggtcgccgt c
//
LOCUS       DATACA010000455         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2230486, whole genome shotgun sequence.
ACCESSION   DATACA010000455 DATACA010000000
VERSION     DATACA010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2230486"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VHK28_09255"
     CDS             complement(<1..511)
                     /locus_tag="VHK28_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142440.1"
                     /translation="MIVRWLAAVAAFLAVAVIGIVVLGNFQTGVDPDGFFDTEGANSF
                     ALIGAIALVAAFVAYAVTEYAQTGALSSISRQFDTRTIVLMPIAIAINIILGATVANA
                     LKIPIYLDSIGTILVGALAGPIPGALTGFLANILWTYVVPPPFQNGPAAAFAVVAAVI
                     GLMAGLAGRM"
     gene            complement(546..1529)
                     /locus_tag="VHK28_09260"
     CDS             complement(546..1529)
                     /locus_tag="VHK28_09260"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX2142441.1"
                     /translation="MEPPSAEIRSAPRQLIMDVDTGIDDMVALLIAATAPELNLRGVT
                     CVAGNVEIHHVARNTGKLLSMVGRADVPVSIGAARPLQRRLRTAPDTHGPTGTGYVEL
                     RGEDRKLGTTEYTPIPAPRYLGAHVKRHPGEMTVLALGPLTNLASAALNGIDLADGTR
                     EVIWMGGTLEERGNTTETGEWNACVDPEAVEACLRAGVLDRIFPLDATQDLAFTMDDL
                     RALPETYLAGVVRDAVRYYVGFHRQADGIDGAYLHDPVTLIASLLRPDLVTETVHERL
                     ACDTSSDPHAAGSLYRATDGDRPSVEVATAIDPDGMKAELLERLAHAVSAR"
     gene            complement(1560..>2041)
                     /locus_tag="VHK28_09265"
     CDS             complement(1560..>2041)
                     /locus_tag="VHK28_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX2142442.1"
                     /translation="TAFGCPYEGEVDARAVVEVSQRLLALGVDELAVSDTVGAAAPAD
                     VRRVLQALRAAGIGPEAVALHLHDTRGTALANVMAALDAGVTAFDASAGGTGGSPYAP
                     GAAGNLATEDLVYLLDRQGLRHGADLDGVLAAARFAATALGRAEVGSKVGRAGGWPR"
BASE COUNT          289 a          723 c          741 g          288 t
ORIGIN      
        1 ccattcgccc ggcgaggccg gccatcaggc cgatgaccgc cgccaccacg gcgaacgcgg
       61 cggccgggcc gttctggaag ggtggcggca cgacgtaggt ccacaggatg ttggccagga
      121 agccggtcag cgcgccgggt atcggtcccg ccagggcgcc gaccaagatc gtcccgatgc
      181 tgtcgaggta gatcgggatc ttcagcgcgt tggccacggt ggcgcccagg atgatgttga
      241 tggcgatggc aattggcatg agcacgatgg ttcgggtgtc gaactgccgg gagatggagc
      301 tgagggcgcc ggtctgcgcg tactcggtga ccgcgtaggc cacgaaggcg gccaccaggg
      361 cgatggcgcc gatcagcgcg aacgagttgg cgccctcggt gtcgaagaag ccgtccggat
      421 cgacacccgt ctggaagttg cccaggacga cgatgccgat caccgctacg gccaggaatg
      481 ccgccacggc ggcgagccag cgcacgatca cgtgtgggat cctccttcca gtgagccggc
      541 tggcgctatc gcgccgacac ggcgtgtgcc agccgctcga gcagctcggc cttcattcca
      601 tcggggtcga tcgcggtggc cacctcgacc gatgggcggt cgccatcggt cgcccggtag
      661 aggctgccgg ccgcatgcgg gtccgaggag gtgtcgcagg ccagccgctc gtgcaccgtc
      721 tcggtcacca ggtctgggcg cagcagggac gcgatcagcg tgaccgggtc gtgcaggtag
      781 gcaccgtcga tgccgtccgc ctggcggtgg aagccgacgt agtagcgcac cgcgtcgcgg
      841 accacgccgg ccaggtaggt ctcaggcagg gcgcgcaggt cgtccatggt gaaggccaga
      901 tcctgggtcg cgtccagcgg gaagatgcgg tccagtaccc cggcccgcag gcaggcctcc
      961 accgcctcgg ggtcgacgca tgcgttccat tcgccggtct cggtggtgtt gccgcgttcc
     1021 tcgagggtgc cgcccatcca gatcacctcc cgcgtgccgt cagccaggtc gatcccgttg
     1081 agggccgcgg atgccaggtt ggtgagcgga ccgagggcca ataccgtcat ctccccggga
     1141 tgccgcttga cgtgcgcgcc caggtagcgc ggcgccggaa tgggcgtgta ctcggtggtg
     1201 cccagcttgc ggtcctcgcc gcgcagctcg acgtacccgg tgccggtcgg gccatgggtg
     1261 tcgggagcgg tcctgaggcg gcgctggagg ggtcgggcgg caccgatgct gaccggcacg
     1321 tcagccctgc ccaccatgga cagcagcttc ccggtgttgc gcgccacgtg atggatctcc
     1381 acgttgccgg ccacgcaggt cacgccccgc aggttgagct cgggcgcggt ggcggcgatc
     1441 agcagcgcga ccatgtcgtc gatcccggtg tcgacatcca tgatcagctg gcgcggggct
     1501 gaccggatct cggcggacgg cggctccacg tctcgcatcc tagcgtgcca cgccagccct
     1561 caacgcggcc aaccgccagc ccgcccgacc ttgctgccga cctccgccct gcccagcgcc
     1621 gtggccgcga agcgagccgc cgccagcacg ccgtccaggt cggccccgtg gcgcaggccc
     1681 tggcggtcga gcaggtagac gaggtcctcg gtggccaggt tgccggcagc gccgggcgcg
     1741 tacggcgagc cgccggtacc cccggccgag gcgtcgaagg cggtcacccc cgcatccagc
     1801 gcggccatca cgttggccag cgcggtgccc cgcgtgtcgt gcaggtgcag cgccaccgcc
     1861 tccgggccga tcccggccgc ccgcagcgcc tgcagcacgc gacgcacgtc cgccggggcc
     1921 gcggcaccca ccgtgtcgct gaccgccagc tcgtcgacgc cgagcgcaag cagccgctgg
     1981 ctgacctcga ccacggcgcg cgcatccacc tcgccctcgt acgggcagcc gaaggcggtg
     2041 g
//
LOCUS       DATACA010000456         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2149908, whole genome shotgun sequence.
ACCESSION   DATACA010000456 DATACA010000000
VERSION     DATACA010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2149908"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VHK28_09270"
     CDS             <1..266
                     /locus_tag="VHK28_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142443.1"
                     /translation="REQLLAIARRHRRPAVAIVFALPLETCLERNLARRDRRLPAAAI
                     RRQYRWLRDSLREIPNEGFAGVWVLESAEEVAAARVEIVPATA"
     gene            complement(263..1168)
                     /locus_tag="VHK28_09275"
     CDS             complement(263..1168)
                     /locus_tag="VHK28_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142444.1"
                     /translation="MTTSDAGWQVTTEPAVLLPGRRAVVRVNYTPSRDHESRGVHATI
                     RCVERYRYDTHETSTDANGVTHTRTVTHDDEDELHRFGVELAGPRRFSAGGTQIWEFD
                     WEVPGLGPPTFEGDVLRCDWALETKADIRMRPDERAEHRLLVAQPTALLRAGVVHTGQ
                     YGLYEEAPANLDHLPAQISLNPVPLCLGAPFSGAFTVQTAEPVEVQEVRLELRTKVEV
                     TVSGGHEEEITVARGRLEHGDRGLFGGEFAVHRFVADATEAWLPSVDLAHGRARATFH
                     VILARAWAPDIHYVRDVALATTAEL"
     gene            complement(1554..2552)
                     /locus_tag="VHK28_09280"
     CDS             complement(1554..2552)
                     /locus_tag="VHK28_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX2142445.1"
                     /translation="MRQLPRLLRHRDRDLRPGEVQVPATAEEPRIQMLEAGGVRWYHA
                     EDPRAADTGWLAEHFAFHPLDLEDVASINQRPKLDVYDEYLFLVVHFPRFVKATGRLH
                     AAELDIFIGPGYVVTLPEEAIVPLPALWERAQEQPDFRAELFAGGSGRLLYEILDRGM
                     DACFPMLGQLGRKLRRLEDDIFEGRSSESVREISNTKQEIINFRAIIRPQRGVFRALE
                     RVKQRHFGDDLDVYFDDLTDASERIWDVLENFKEIVEALESTNESVLTHRLNDVLRVL
                     TAASVVLLPLTLIASIWGMNVQVPGEGDAAPFYFILAGMAFILVTLIAWFRRRGWL"
     gene            complement(2632..3996)
                     /locus_tag="VHK28_09285"
     CDS             complement(2632..3996)
                     /locus_tag="VHK28_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142446.1"
                     /translation="MRRPLVGLVVAAFVVAGCEADAPSPSLAPQPPATTLPDRHSDDA
                     SLIATLPNDEHSLDFANSDMAFWGDLLVQGSYDGFRVVGISDPGHPETLAEVACRGPQ
                     GDVSMWEHLVFVSVDRPQSSTGCESEDAEDADPLFLPDQGGWEGIRIFDVMAPSAPRL
                     VGTVPTDCGSHTHTLVPDVANGRVLLYVSSYALLQDERLAPECINPHGHISVVDVPLD
                     DPGAAALITQPHLFDTPAWEVAPGAPPNWHSTAGCHDITVFIPLRLAAAACLSEGQIW
                     DLSNPERPMAIAHVDLPEVAFWHSAAWSNDGSVVAFGDEDLSETGCSDDPGGAIWFYR
                     VVDPRAPELAGHFSLDRYARPEVCSAHMFNVVPGVERDLLVGGWYAGGTSVVDFTDPS
                     APREVAYYDVGGDQPGDVWSSYWYRGHVWVSDHHRGLDVLEVEMPELAGAVFLDRLNP
                     QTQE"
     gene            complement(3993..>4451)
                     /locus_tag="VHK28_09290"
     CDS             complement(3993..>4451)
                     /locus_tag="VHK28_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142447.1"
                     /translation="ARVAVHGSRRALVVTGLSLIVAGIGLATSVLVSGVPVWVAGAAW
                     AVAGLGMGLAFSTATLSAIEGAPVGAEGSTSGAVQLANILGVAVGTGVAGAVVAVSGP
                     TLGLAPGIAIANLLMLVACAAGIAVVGRLPDTPPSGAAARSMPAEHGPAL"
BASE COUNT          699 a         1576 c         1536 g          640 t
ORIGIN      
        1 cacgcgagca gctgctggcc atcgcccgcc gccaccgacg cccggcggtg gccatcgtct
       61 tcgccctgcc gctcgagacg tgcctggagc gcaacctcgc ccgccgggat cggcgcctgc
      121 ccgcagcagc catccgacgc cagtaccgat ggcttcgcga ctcactgcgc gaaattccga
      181 acgagggatt cgccggcgtg tgggtcctcg agtcggcgga ggaagtcgct gcggcccgtg
      241 tcgagatcgt tccggcgact gcctagagct ctgcggtggt ggccagcgcc acgtcgcgga
      301 cgtagtggat gtccggcgcc caggcccgcg ccaggatgac gtggaacgtg gcccgggcgc
      361 gcccgtgggc caggtcgacg gacggcagcc aggcctcggt ggcatccgcc acgaagcggt
      421 ggacggcgaa ctcgccgccg aacagccccc ggtcgccatg ctcgaggcga ccgcgggcca
      481 cggtgatctc ctcctcgtgc ccaccgctga cagtgacctc caccttggtg cgcagctcga
      541 ggcggacctc ctgcacctcg accggctctg cggtctgcac ggtgaaggct ccgctgaatg
      601 gcgcgcccag gcagagcggg acaggattga ggctgatctg cgctggcaga tggtccaggt
      661 tggccggcgc ctcctcgtac aggccgtact ggccggtgtg caccacgccg gcgcgaagca
      721 gggcggtcgg ctgtgccacc agcagacggt gctcggcacg ctcgtccggc cgcatgcgga
      781 tatcggcctt ggtctccagg gcccagtcgc agcgcaggac atcgccctcg aaggtcggcg
      841 gcccgaggcc ggggacctcc cagtcgaact cccagatctg ggtgccgccc gcactgaaac
      901 ggcgtgggcc ggcgagctcc acgccgaagc ggtgcagctc gtcctcgtcg tcgtgcgtga
      961 ccgtccgggt gtgcgtcacg ccgttggcat cggtgctcgt ctcgtgggtg tcatagcggt
     1021 agcgctccac gcagcggatg gtggcgtgta cgccacgcga ctcatggtcg cgcgacggcg
     1081 tgtaattcac gcgtaccacg gcgcggcgcc cgggcagcag caccgccggc tcggtcgtca
     1141 cctgccagcc ggcgtccgag gtcgtcaagc gatcaatccc tagccggcgg tcgccggggc
     1201 acacgaaatg tcccgaagga actgcagacc cgaaccgatg gtgcgagacc accgacccgg
     1261 taccggaact cttgatgcaa tctcgtgatc cggtatccgc tgccccttcg ggacgaccaa
     1321 aggataggcg cgacggttgg gccggggcaa taccacttat ccccgacttt cacggccctt
     1381 tcgccgcttg tccacagcca atccccactt gatgccccga tgccccaccg gggtgctggt
     1441 ggggcatcga agtgtcgcct gccgagccgg tcggtgcggc tcggaccaac caggcgacac
     1501 aggtggtacc cgtccagtgg caccgatgtt caccttttgc ctggcaggac gagctagagc
     1561 cagccgcggc gccggaacca ggcgatgagc gtcaccagga tgaacgccat cccggccagg
     1621 atgaagtaga agggggccgc gtcaccctcg ccgggcacct ggacgttcat gccccagatg
     1681 ctcgcgatca gggtcagtgg cagcagcacc acgctggccg cggtcagcac ccgcagcacg
     1741 tcgttcaggc ggtgggtgag caccgattcg ttggtcgact cgagcgcttc cacgatctcc
     1801 ttgaagttct cgagcacgtc ccagatgcgc tcggaggcgt cggtcaggtc gtcgaagtac
     1861 acgtccaggt cgtcaccgaa gtggcgctgc ttgacgcgct ccagggcgcg aaacacgccg
     1921 cgctgcgggc ggatgatcgc gcggaagttg atgatctcct gcttggtatt gctgatctcg
     1981 cgcacgcttt cgctggagcg tccctcgaag atgtcgtcct ccagccgccg cagcttgcga
     2041 cccagctgtc ccagcatcgg gaagcaggca tccatgccgc gatccaggat ctcgtacagc
     2101 agccgcccgg agccgccggc gaagagctct gcgcggaagt ccggttgctc ctgcgcccgc
     2161 tcccagaggg ccggcagcgg cacgatcgcc tcctccggca gggtaaccac gtagccggga
     2221 ccgatgaaga tgtccagctc cgcggcatgc agccggcctg tggccttcac gaagcgcggg
     2281 aaatgcacga ccaggaagag gtactcgtcg tagacgtcca gcttcggccg ctggttgatg
     2341 ctggccacgt cctccaggtc cagggggtgg aaggcgaagt gctccgccag ccagccggtg
     2401 tcggcggcgc ggggatcctc cgcgtggtac cagcgcacgc cgcccgcctc cagcatctgg
     2461 atgcgcggct cttcggcggt ggccggcacc tgaacctcgc cgggccgcag gtcccgatcg
     2521 cgatgtcgca gcaggcgcgg tagctgtctc accccctgag cgtacgccgt cggcgagctg
     2581 aatggagcgg ccaattgctc ggcggctgat caccgcggcg gtcgctccgc atcattcctg
     2641 ggtctgcgga ttcagccggt cgaggaagac cgcgcccgcg agctccggca tttccacctc
     2701 cagcacgtcc aggccgcgat ggtgatccga gacccagacg tggccgcggt accagtagga
     2761 cgaccagacg tcgccgggct gatcgcctcc cacgtcgtag taggcgacct ctcgcggcgc
     2821 cgagggatcg gtgaagtcga ccaccgaggt gccgccggcg taccagccgc cgaccagcag
     2881 gtcccgctcc acgccgggaa cgacgttgaa catgtgggcc gagcagacct ccgggcgggc
     2941 gtatcggtcc aggctgaagt ggccggcaag ctcgggggcg cgcgggtcca ctacgcggta
     3001 gaaccagatg gcgccgcccg ggtcatcgct gcagcccgtc tcggagaggt cctcgtcgcc
     3061 gaaggcgacc acgctgccgt cgttgctcca cgcagcggag tgccagaagg ccacctccgg
     3121 caggtcgacg tgggcgatgg ccatcggtcg ttccgggttg gagaggtccc agatctggcc
     3181 ctcgctcagg caggcggccg ccgccaggcg cagagggatg aagacggtga tgtcgtggca
     3241 gccggcggtg gagtgccagt tcggcggggc gccgggggcg acctcccagg cgggggtatc
     3301 gaacaggtgc ggctgggtga tcagcgcggc ggcgccggga tcatccagtg gcacgtccac
     3361 cacgctgatg tgaccgtgcg ggttgatgca ctccggcgcc agccgctcgt cctgcagcag
     3421 cgcgtacgag ctgacgtaga gcagcacgcg gccattagcc acatccggca cgagcgtgtg
     3481 ggtgtgcgag ccgcagtcgg tcggtacggt ccccaccagc ctcggcgccg acggtgccat
     3541 gacgtcgaag atgcgaatcc cctcccagcc gccctggtcc ggcaggaaca gcgggtcggc
     3601 gtcctccgcg tcctccgact cgcaccccgt gctgctctgc ggccggtcga cgctcacgaa
     3661 gacgaggtgc tcccacatgg agacgtcgcc ctgcggcccg cggcaggcaa cctcggccag
     3721 ggtctcggga tggcccggat cgctgatgcc caccacccga aacccgtcat agctgccctg
     3781 caccagcagg tctccccaga aggccatgtc cgagttggca aagtccaggg aatgctcgtc
     3841 gttcggaagc gtggcgatca ggctcgcgtc gtccgagtgc cggtccggga gggtcgtcgc
     3901 cggcggctgg ggggccaggg acggcgaagg cgcatccgcc tcgcatccgg caacgacgaa
     3961 tgccgccacc accagcccga ccagtggcct cctcacagcg ccggcccgtg ctcggcgggc
     4021 atggaccgag cggccgcccc ggacggcggc gtgtccggga ggcgcccgac taccgcgatc
     4081 ccggccgcgc aggcgaccag catcagcagg ttcgcgatgg cgatgcccgg cgccaggccg
     4141 agggtcggcc ccgagaccgc caccaccgcc ccggccactc cggtccccac cgccactccc
     4201 agaatgttcg ccagctggac ggcgccggag gtggaccctt ccgcgccgac cggcgccccc
     4261 tcgatcgctg acagggtggc ggtcgagaag gcgaggccca tccccaggcc ggcgactgcc
     4321 caggccgcgc cggccaccca gacgggcacg ccggacacca ggaccgatgt cgccagcccg
     4381 atgccggcca cgatcaaaga caaaccggtg acgacgagcg cccgccgaga gccgtgcacc
     4441 gcgacccgtg c
//
LOCUS       DATACA010000457         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1347904, whole genome shotgun sequence.
ACCESSION   DATACA010000457 DATACA010000000
VERSION     DATACA010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347904"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VHK28_09295"
     CDS             complement(<1..1703)
                     /locus_tag="VHK28_09295"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2142448.1"
                     /translation="MIAPLALIGLVGLPIIVAFYMLRLRRRDVPVGSTFLWQQLVRDV
                     EANAPWQRLRFSWLLLVQLLIALLAVGAAVRPFGTVSSDLAANVVLIIDTSASMGAID
                     PEGQRMAQAREAARAVIGEVPEGGRLTVVAADDSAQVLVSETDDRAAALAAVDGIQAT
                     EVPGDLTDAFALASALAARDSDSSVVVVTDANADRLPRVGIGAPVRVERVGSTDDNQA
                     IAALSAIRRSGGSQVDLFVAVSNPSTSEVSRRLEVYADGVLVDARDLTLPAEQRTEAL
                     IASVPPSAFLVEARLDGEDALAVDDSALALVPSEEVVPALLVSDRNTYLENALALLPR
                     LELYAVGPAGYEEAIEAGTEDGRPYGMVVFDRFVPDAPPPLPTLWIGPDQDGPFGTVG
                     AAVDGPALAPIAADDPLLRFLDLSTLHVGRARAVQLADGMRAAVSSTEGEPLIAAGRV
                     DGRTLALLTFELGETDLPLQVAFPLLVSSLTDFLLPPTDGILPPSVDLGEALVVQLNP
                     ATSRVRIDTTPVNQDPGPAGQALELDVVGGRVTLPGARRPGVVRLSALDGLAEGADPV
                     LG"
BASE COUNT          264 a          628 c          620 g          209 t
ORIGIN      
        1 ccgagcaccg gatcggcgcc ctccgccagc ccgtccagcg cgctgaggcg caccacgccg
       61 ggacgccgcg cgccgggcag cgtcacccgg ccaccgacca catcgagctc aagtgcctgt
      121 cccgcgggac cgggatcctg gttcaccggc gtggtgtcaa tgcggacgcg gctggtggca
      181 gggttcagtt gcaccaccag cgcctcgccc aggtccacgg acggcgggag gatgccgtcg
      241 gtcggcggca gcaggaagtc ggtaaggctg ctgaccagca gcgggaacgc gacctgcagc
      301 ggcaggtccg tctcgccgag ctcgaaggtg agcagcgcca gggtgcgtcc gtcaacccgc
      361 ccggcggcga tcagcggctc gccctcggtg ctcgagacgg cggctcgcat gccgtccgcc
      421 agctgcacgg ccctcgcccg cccaacgtgc agggtggaaa ggtccaggaa gcgcagcagc
      481 gggtcgtccg cggcgatcgg cgccagcgca ggcccatcta ccgcggcgcc cacggtgccg
      541 aacgggccgt cctggtcggg accgatccac agcgtgggca gcggcggcgg cgcgtccggc
      601 acgaagcgat cgaagacgac catcccgtac ggccggccgt cctccgtgcc ggcctctatc
      661 gcctcctcgt atccggccgg accgaccgcg tacagctcca aacggggcag cagggccagg
      721 gcgttctcca ggtaggtgtt ccggtcgctg accagcaggg ccggcacgac ctcctcggac
      781 ggcacgagtg ccagcgccga gtcgtccacg gccagcgcat cttccccgtc gagccgggcc
      841 tcgaccagga aggcagaggg cgggacgctg gcgatcagtg cctcggtgcg ctgttccgcg
      901 ggcagcgtca ggtcgcgggc atcgaccagc acgccgtcgg cgtagacctc cagccggcgg
      961 ctgacctcgc tggtagacgg gttgctgacg gccacgaaca ggtcgacctg cgagccgccc
     1021 gaacgccgga tggcggacag ggcggcgatc gcctggttgt catcggtcga gccgacccgt
     1081 tcgaccctga ccggtgcacc gatgccgaca cgcggcaggc ggtcggcgtt cgcatcggtc
     1141 accaccacca cgctggagtc cgagtcgcga gcggccagcg cagaggccag ggcgaaggca
     1201 tcggtcaggt caccgggcac ttccgtggcc tggatgccgt ccacggccgc cagggccgcc
     1261 gcgcgatcat cggtctcgct gaccaggact tgggccgagt cgtcggcggc gaccaccgtc
     1321 agccggccgc cctccggtac ctcgccgatc acagcgcggg ccgcctcgcg cgcctgggcc
     1381 atgcgctggc cctccgggtc gatggcgccc atggaggccg aggtgtcgat gatgagcacc
     1441 acgttggccg cgaggtcgga gctgaccgtg ccgaagggcc gtaccgcggc gcccaccgcc
     1501 agcagcgcga tcagcagctg caccagcagc agccagctga agcgcagccg ctgccacggg
     1561 gcgttggcct cgacgtcgcg caccagctgc tgccacagga aggtgctgcc caccggcacg
     1621 tcgcggcgcc ggaggcggag catgtagaag gccacgatga tcggcagacc gaccagcccg
     1681 atgagcgcca gcggagcgat cactggactg atgcctccgg g
//
LOCUS       DATACA010000458         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_143316, whole genome shotgun sequence.
ACCESSION   DATACA010000458 DATACA010000000
VERSION     DATACA010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143316"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VHK28_09300"
     CDS             complement(<1..687)
                     /locus_tag="VHK28_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142449.1"
                     /translation="MPESPSSRAARGIRDELLMVLVPVGLVILTMVVLLDPSVAPGAI
                     NLRLALAVDLTATLVAAAIAILAWVHFREGREPAALLRGSAFVALGSLNALFAAAAVV
                     GAEGAFGLDAADPGQLPLWSTATSRTGAAALLAMAGIAALRGSTVVRWPPALTFWLPG
                     LTVVGLTLAVAVLQVSLPTLIPPEQIARLLEDPRQPLEAAAAPGYVAWQMLIGLCYLA
                     AAALSYRVYRR"
     gene            complement(731..>2025)
                     /gene="gatB"
                     /locus_tag="VHK28_09305"
     CDS             complement(731..>2025)
                     /gene="gatB"
                     /locus_tag="VHK28_09305"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX2142450.1"
                     /translation="NRRAVELVILTGLALGCEIQTDAVRFERKNYFYPDLPKGYQISQ
                     YALPLSANGQLLVPVPSEGTDVTVGITRAHLEEDTARLLHGGRGHTLVDFNRAGVPLM
                     EIVTEPDIRSPAQARAYGETLRDILRHIGASDGDMETGSMRIEGNVSLRPMGTERFGT
                     KVEVKNLNSFRSLERAMEFEVARHAEALEKGERLVQETRGWDESAGRTISQRSKEEAN
                     DYRYFPEPDLPPLRPSAAWVEELRTALPELPAARRSRYVEQLGLSAYDAGVLTADVAL
                     ADYFDAVVGAGVSAKAAANWMTGEFSRLLNQHAAEGLRASGVALRPEGLAELIGEVEG
                     GRVSATSAKEVLARTFVSGESPMDVIRAEGLAQVSDAGLIGREVAAVLDEFPSQVAEY
                     RGGKQQIYGFLVGQVMKRTAGRADARLVNEELRRQLDG"
BASE COUNT          302 a          735 c          709 g          279 t
ORIGIN      
        1 ccgtcggtag acccggtacg acagcgccgc ggctgccagg tagcacaggc cgatgagcat
       61 ttgccaggcc acgtacccgg gcgcggcggc ggcttcgagc ggctgccgcg ggtcctcgag
      121 caggcgcgcg atctgctccg gagggatcaa ggtcgggagc gagacctgca gcacggcgac
      181 cgccagggtg aggccaacca cggtcagccc gggcagccag aaggtgaggg ccggcggcca
      241 gcgaaccacg gtcgagccgc gcagggcggc gatgcccgcc atcgcgagga gggcggcagc
      301 ccccgtgcgc gaggtcgcgg tcgaccacag cggcagctgc ccgggatcgg ctgcatccag
      361 cccgaatgcg ccctctgcac ccaccacggc ggcggcggcg aacagggcgt tgaggctgcc
      421 cagcgccacg aatgcgctgc cgcggagcag ggcggcgggc tcgcgcccct cccggaagtg
      481 gacccaggcc aggatggcga tggcggccgc caccagcgtg gcggtcaggt cgacggccag
      541 cgccagccgc aaattgatgg cgccgggcgc cacgctcggg tcgagcagga cgaccatggt
      601 cagtatgacc aggccgaccg gcacgagcac catcagcagc tcgtcccgga ttccgcgggc
      661 agcgcgcgaa ctgggcgatt cgggcacggc cgaaggatac agaccccggt ttgcgagtta
      721 catggcccga tcagccgtcc agctgtcgtc ggagctcctc gttgaccagg cgggcatcgg
      781 cccggccggc ggtgcgcttc atgacctggc cgaccaggaa gccgtagatc tgctgcttcc
      841 cgccgcggta ctcagccacc tgggacggga actcgtcgag cacggccgcc acctcgcgac
      901 cgatgagccc ggcgtcactc acctgggcca gcccctcggc gcggatgacg tccatcggcg
      961 actccccgga gacgaaggtg cgcgccagga cctccttggc gctggtggcc gatacccggc
     1021 ccccctccac ctcaccgatg agctcggcca atccctccgg ccgcagcgcg acgcccgagg
     1081 cccgcagccc ctccgcggcg tgctggttca gcagccggct gaactcgccg gtcatccagt
     1141 tggcggctgc cttggcgctc acgccggcgc cgaccaccgc gtcgaagtag tcggccagcg
     1201 cgacgtcggc ggtcagcacg cccgcgtcgt aggccgagag gcccagctgc tccacgtacc
     1261 ggctgcggcg ggccgctggc agctccggca gcgcagtccg cagctcctct acccaggccg
     1321 cggacgggcg cagcggcggc aggtccggct cggggaagta acggtagtcg ttcgcctctt
     1381 ccttgctgcg ctggctgatg gtgcgcccgg cgctctcgtc ccagccgcgc gtctcctgca
     1441 ccagccgctc ccccttctcc agcgcctcgg cgtgccgggc gacctcgaac tccatggcgc
     1501 gctccagcga gcggaagctg ttgaggttct tgacctcgac cttggtgccg aagcgctcgg
     1561 tgcccatcgg tctcaggctg acgttgccct cgatgcgcat ggacccggtc tccatgtcac
     1621 cgtccgatgc accgatgtgg cgcaggatgt cgcgcagcgt ctccccgtag gcccgcgcct
     1681 gggcaggcga gcgaatgtca ggctcggtca cgatctccat cagcggcacg cccgcgcggt
     1741 tgaagtccac cagcgtgtgg ccccgaccac cgtgcagcag gcgcgcggtg tcctcctcga
     1801 ggtgggcgcg ggtgatgccg accgtgacat cggtcccttc agaggggacg ggcaccagca
     1861 gctgcccatt cgccgacagc ggcaaggcgt actggctgat ctgatagccc ttgggcaggt
     1921 ccgggtaaaa gtagttcttg cgctcgaagc ggaccgcgtc ggtctggatc tcgcagccca
     1981 gcgccaggcc ggtcaggatc accagctcga ctgcgcggcg attga
//
LOCUS       DATACA010000459         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2632314, whole genome shotgun sequence.
ACCESSION   DATACA010000459 DATACA010000000
VERSION     DATACA010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632314"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1254
                     /gene="add"
                     /locus_tag="VHK28_09310"
     CDS             211..1254
                     /gene="add"
                     /locus_tag="VHK28_09310"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX2142451.1"
                     /translation="MEGSEIDAAELRALPKAHLHQHLDGELRPETAVELAPDIGLSLT
                     LDEARTRMVAPDRSANQAELLSYFDLPIALLQRPEALARAAAELVEDEAADGVRYLEV
                     RWAPRLHLTAGHSVRDVIDAVAQGVADARSRLGAAAPMVVLIVTAMRSHPPAANVLLA
                     QTAAAYGPPVVGFDLAGPEAEYPAPPHAAAFRAAEEGGLALTAHAGEVSGPERIREVL
                     AFGVRRIAHGATAWIDPQVVALLRERDVTLDLCPTSNLQAGTFPSLREHPLVQLHRSG
                     VSVTLSTDDRTVSASTLSMEMARVATAQGLTRAELAEIAVNGFRRGFGAYSERSALAR
                     EAAVAWDGWAAIS"
     gene            complement(1247..>1764)
                     /locus_tag="VHK28_09315"
     CDS             complement(1247..>1764)
                     /locus_tag="VHK28_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX2142452.1"
                     /translation="AVAASSAAPRDWNGGPLPARYDAVITGLDPEIDYRRLGIAAAAI
                     REGAAFIATNADVRYPTPEGFVPGAGSIVAALRATTGVEPLVVGKPQPAMLLAILEEA
                     AVPGAEALVIGDNPDADIRAARGAGISSVLVLTGVADAASAEALDGDRRADWVAEDPV
                     GVARLLGLSLN"
BASE COUNT          232 a          635 c          650 g          247 t
ORIGIN      
        1 gccgtgctcg atggcggcct ccagcgcctc ctccaggcgg gccaggccct cgtcagcttt
       61 gcgagccacc gatcgcagcg tctcttggcg ccgctcggcg cgcggcgggt ccgcggccgc
      121 agcatcggcc ggccgcatgg cgagcagccc gccgagcacg actgcggagg ccactccgat
      181 caggactcca ccgctccgta tcatgcgcac gtggaaggct ccgagatcga cgccgccgag
      241 ctccgtgcgc tccccaaggc ccacctgcac caacacctcg acggcgaatt gcgccccgag
      301 acggccgtgg agctggcccc ggatatcggc ctttcgctga ccctggacga ggcgcggacg
      361 cggatggtgg cgcccgacag gtccgcgaac caggcggagc tgctgtcgta cttcgacctg
      421 ccgatcgccc tgctgcagcg gccggaggcg ctggcccgcg cggcggccga gctggtggag
      481 gacgaggcgg cggatggcgt gcggtacctc gaggttcgct gggcgccccg gctgcacctg
      541 accgccggcc actcggtgcg cgacgtgatc gacgcggtgg cccagggcgt ggccgatgcc
      601 aggtccaggc tgggtgcggc ggcgcctatg gtggtcctga tcgtgaccgc catgcgctcg
      661 cacccgccgg ccgccaacgt gctgctggcc cagaccgcag ccgcgtacgg cccgccggtg
      721 gtcggcttcg acctggctgg gccggaggcc gagtaccctg ctccgccgca cgccgccgcc
      781 ttccgggccg cggaggaggg agggctggcg ctgacggcgc atgccggcga ggtctcgggg
      841 ccggagcgca tccgggaagt gctggccttc ggggtgcgac gcatcgccca tggagcgact
      901 gcctggatcg atccgcaggt ggtcgcgctg ctgcgcgagc gggacgtgac ccttgacctg
      961 tgccccacct ccaacctgca ggccggcacc ttcccctcgc tgcgggagca tccattggtg
     1021 cagctccatc ggtccggggt gagcgtgaca ctgtcgaccg acgaccgaac ggtctccgcc
     1081 tccaccctgt cgatggaaat ggcgcgggta gcgaccgcgc agggcctgac gcgcgcggag
     1141 ctggcggaga tcgcggtcaa cggattccgt cgcggattcg gcgcgtactc cgagcggagc
     1201 gctctggcac gtgaggcggc cgtggcctgg gacgggtggg ccgccatcag ttgagggaaa
     1261 gccccagaag ccgggccacg ccgaccggat cctctgcgac ccagtctgcc ctccggtcgc
     1321 cgtccagggc ttcggcgctg gcagcgtccg caaccccggt caggaccagg accgatgaaa
     1381 tccccgcccc tcgcgcggcg cgaatgtcgg catccgggtt gtcgccgatc accagtgcct
     1441 cggctccggg tacggcggcc tcctccagga tggcgagcag catggccggc tggggcttgc
     1501 cgacgacgag cggctcgaca ccggtagtgg cgcgcagcgc cgccacgatg ctgccggcac
     1561 cgggtacgaa gccctccggc gtggggtagc ggacgtcggc attggtggcg atgaaggctg
     1621 ctccctcgcg aatggcggcg gcggcgatcc ccagccgccg gtagtcgatc tccgggtcca
     1681 agccagtgat caccgcgtcg tagcgtgcgg gcagcgggcc tccgttccag tcccgtgggg
     1741 ccgcactgga ggcggcgacg gcct
//
LOCUS       DATACA010000460         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2231206, whole genome shotgun sequence.
ACCESSION   DATACA010000460 DATACA010000000
VERSION     DATACA010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2231206"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /gene="purM"
                     /locus_tag="VHK28_09320"
     CDS             complement(<1..860)
                     /gene="purM"
                     /locus_tag="VHK28_09320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2142453.1"
                     /translation="MRRRYREAGVDLDEARRAVELIGRAAASTATPSVIGGVGGFGSL
                     FRFDPAPYPDPVLVASTDGVGTKLKVALTLGRHDTIGVDLVNHCINDIAVQGAEPLFF
                     LDYLGTGRLRAEVAEEIVGGIAAACAAAGVALVGGETAELPDLYQGDDYDLAGFIVGV
                     VSRREVIDGKGVRGGDALMGLPSSGLHTNGYSLARRVLPAAEWDRSAPGLERSIGEEL
                     LEPHRSYLDEIRILRRALRRSGSDLLALAHLTGGGWVDNIPRTLPAGLGVEVESGSWP
                     VPRIFSLIQER"
     gene            complement(871..>1591)
                     /locus_tag="VHK28_09325"
     CDS             complement(871..>1591)
                     /locus_tag="VHK28_09325"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2142454.1"
                     /translation="HSHRPPAPAAAERLCVFEFVYLARPDSVIGGRLLYEARLEMGRR
                     LALEHPVEADVVIPVPDSAIPAAIGYAEQAGLPFREGLIKNRYVGRTFIQPVQRSREA
                     SVQMKFNPLPEVLANKRVVVVDDSIVRGTTTGPIVSMLRRAGAREVHVRIHSPQMRFP
                     CYMGVDTGRRSELIAANQGLDEIRRTIGADSLGYLSQEGLLAAIGRERDGERRHCTAC
                     FDGRYPVDVPLDVDKFALERG"
BASE COUNT          231 a          593 c          550 g          217 t
ORIGIN      
        1 cgctcctgga tcaggctgaa gatgcgcggc accggccatg agccgctctc cacctccacg
       61 cccagcccgg cggggagcgt gcgcgggatg ttgtccaccc agccaccacc ggtgaggtgg
      121 gcgagtgcca gcaggtcgct ccccgagcga cgcagtgcgc ggcgcaggat gcggatctcg
      181 tccaggtagg accgatgcgg ctccagcagc tcctccccga tgctgcgctc aagccccggg
      241 gcgctgcggt cccactcggc cgccggaagg acgcggcgcg ccagcgagta gccgttggtg
      301 tgcaggccgc tggagggaag gcccatcaac gcgtcgccgc cgcggacgcc cttgccgtcg
      361 atcacctcgc ggcgcgagac cacgcccacg atgaagccgg cgaggtcgta gtcgtcgccc
      421 tggtacaggt ccggcagctc ggccgtctcg ccgccgacca gggccacgcc ggccgccgcg
      481 caggcggccg cgatgccgcc cacgatctcc tcggccacct cggcccgcag ccggccggtg
      541 cccaggtagt ccaggaagaa gagcggttcg gccccctgca ccgcgatgtc gttgatgcag
      601 tggttcacca ggtccacgcc gatggtgtcg tgccggccaa gtgtcagcgc caccttcagc
      661 ttggtgccca ccccatcggt cgaggcaacc agcaccgggt cggggtaggg cgccgggtcg
      721 aagcggaaga ggctgccgaa gccgcccacc ccgccgatca ccgacggcgt ggcggtcgac
      781 gcggccgcac ggccgatgag ctccacggcg cgccgcgcct cgtccaggtc cactccggcc
      841 tcgcggtatc gccgtcgcat gtggctcctc ctacccgcgt tccagcgcga acttgtccac
      901 gtcgagcggc acgtccacgg gatagcggcc gtcgaagcag gccgtgcagt gccggcgctc
      961 cccgtcccgc tcgcggccga tggcggccag cagcccttcc tgcgacaggt agccgagcga
     1021 gtcggcgccg atggtgcgcc ggatctcgtc cagcccctgg ttggcggcga tcagttccga
     1081 ccgtcgcccg gtgtcgacgc ccatgtagca cgggaaccgc atctgcgggg agtggatgcg
     1141 cacgtgcacc tctcgggctc ccgcgcggcg caacatggac acgatcggtc cggtggtcgt
     1201 gccgcgcacg atggaatcgt ccaccaccac cacccgcttg ttggccagca cctcgggcag
     1261 cgggttgaac ttcatctgga ccgatgcctc acgcgaccgc tgcacgggct ggatgaaggt
     1321 gcggcccacg tatcggttct tgatcagccc ctcgcggaag ggcaggcccg cctgctctgc
     1381 gtagccgatg gccgccggga tggcgctgtc cggcaccggg atgaccacgt ccgcctccac
     1441 cgggtgctcc agagccaggc ggcggcccat ctccagccgc gcctcgtaca gcaggcggcc
     1501 gccgatgacc gaatccgggc gcgccaggta cacgaactcg aagacgcaca gccgctcggc
     1561 ggcggccggg gccggcggtc gatgcgaatg a
//
LOCUS       DATACA010000461         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2312481, whole genome shotgun sequence.
ACCESSION   DATACA010000461 DATACA010000000
VERSION     DATACA010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2312481"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VHK28_09330"
     CDS             complement(<1..601)
                     /locus_tag="VHK28_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2142455.1"
                     /translation="MSEPIEVSAEQARRFLVSRHLLDPPRSLPAEPESVLRVVDRLGI
                     LQFDPLEVPGARNHDLVLHARIAGYDRSWLDRWLYGPDRRFIEVYNKSLNILPIGELP
                     HYRLAWERADQHYRDRILRDQAQVAEAIVEQIRADGPLSTAAFRQHNHAIDWWWAPTS
                     AARAVMEALFVTGRLGISRREGNRRYYDLIERLVPAELLE"
     gene            681..1424
                     /locus_tag="VHK28_09335"
     CDS             681..1424
                     /locus_tag="VHK28_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142456.1"
                     /translation="MPSPPREPHPLERGGSADTDDAGRRSQRLQILATEHWNLLATRS
                     LSWSESFSRATMFLTVLSGAVVALALVAQATEFGEGFVLFALLILPVVLFVGAATFVR
                     LVEINNEDVHWVYGMNLLRHAYLDMEPDLKPYFVSGFETEADAVIRTYGSHGTGSAWM
                     HGLITTPATIAFVNAVMAAVLVAIALMEWQMAMVPSVVVGAVAFAIAIGLQMAFNAYS
                     ARSYQREHGRRDPAGDVPAGSNDPPPPAV"
     gene            1430..>1720
                     /locus_tag="VHK28_09340"
     CDS             1430..>1720
                     /locus_tag="VHK28_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142457.1"
                     /translation="MLRRLTSPVVLLLLAAAVTVLIAIQTGFVRPYEAPTAFVDRWLQ
                     AIVGRGAEDRGWSQLGERTRESFGSAEEWVAAASAQDWSTFRWEVGSPHALES"
BASE COUNT          259 a          588 c          598 g          275 t
ORIGIN      
        1 gttccagcag ctcggcggga acgagccgct cgatcaggtc gtaataccgt cggttgccct
       61 cgcgccgcga gatgccgagc cggccggtga cgaacagcgc ctccatcacc gcccgcgctg
      121 ccgacgtggg cgcccaccac cagtcaatcg cgtggttgtg ctggcggaag gctgccgtgc
      181 tgagcgggcc atcggcccgg atctgctcca cgatcgcctc ggcgacctgc gcctggtcgc
      241 gcaggattcg gtcgcggtag tgctgatcgg cgcgctccca ggcgagccga tagtgcggaa
      301 gctcgccgat gggcaggatg ttcaggctct tgttgtaaac ctcgatgaac cgccggtccg
      361 gcccgtacag ccatcggtcc agccaactcc ggtcgtagcc ggcgatccgg gcatgcagca
      421 ccaggtcgtg gttgcgggcg ccgggcacct ccagcggatc gaactgcagg attcccagcc
      481 gatccaccac gcgcaggacc gattccggct ccgccggcag cgagcgcggc ggatcgagca
      541 ggtggcgact caccaggaag cgacgggcct gttcggcgga gacctcgatc ggctcggaca
      601 tcggcgtgga tgctagtcga ctgcctcgtc cagggcttga cgcccgcgtc acggcgggca
      661 cactcgaagg catgcagcgc atgccgagtc caccgcgcga gcctcacccg ctggaacgtg
      721 gcggaagcgc ggacaccgat gatgccggcc gccggtcaca gcggctccag atcctggcca
      781 ccgagcactg gaacctgctc gccacccggt cgctgtcgtg gagcgagtcg ttcagccggg
      841 ccaccatgtt cctgaccgtg ctgtccggcg cggtggtggc cctggcgctg gtggcgcagg
      901 ccaccgagtt cggggagggc ttcgtgctct tcgcgctgct gatcctgccg gtggtgctct
      961 tcgtcggagc cgccaccttc gtccgcctgg tggaaatcaa caacgaggat gtccactggg
     1021 tctacggcat gaacctgctg cgccacgcct acctggacat ggagccggac ctgaagccct
     1081 acttcgtgag cgggttcgag acggaggccg atgccgtgat ccggacgtac ggatcgcacg
     1141 gcacggggtc ggcctggatg cacggcctga tcacgacacc ggccaccatc gcgttcgtca
     1201 acgcggtgat ggcggcggtc ctggtggcca tcgcgctcat ggagtggcag atggccatgg
     1261 tgccctcggt ggtcgtaggc gccgtggcct tcgcgatcgc catcggtctg cagatggcgt
     1321 tcaacgccta cagcgcgcga agctaccaac gcgagcacgg acggcgggat ccggccggag
     1381 acgtccccgc cggcagcaac gacccaccgc cgccagccgt ctgagggacg tgctgcgccg
     1441 actcacgtcg ccggttgtcc tcctcctgct ggcggccgcc gtcacggtcc tgatcgcgat
     1501 acagaccggc ttcgtgcgtc cctacgaggc tcccaccgcc ttcgtggacc gatggctgca
     1561 ggcgatcgtc ggtcgcggcg cagaggaccg cggttggtcg cagctcggcg agaggacgcg
     1621 tgagtccttc ggcagcgccg aggagtgggt ggcggccgca tcggcccagg actggagcac
     1681 gttccggtgg gaggtcggct caccacacgc ccttgaatcg
//
LOCUS       DATACA010000462         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1107166, whole genome shotgun sequence.
ACCESSION   DATACA010000462 DATACA010000000
VERSION     DATACA010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107166"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VHK28_09345"
     CDS             complement(<1..371)
                     /locus_tag="VHK28_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2142458.1"
                     /translation="MRITTIGLDLAKHVFQVHAVDEAGGVVLRKRLRRSQMLAFFAGL
                     PACVIGIEACATAHYWARELRALGHEVRLMPPHYVKAYVKRGKNDAADAAAICEAVTR
                     PSMRFVPVKAAEQQAALMIHRA"
     gene            complement(451..1030)
                     /locus_tag="VHK28_09350"
                     /pseudo
     CDS             complement(451..1030)
                     /locus_tag="VHK28_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779117.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1051..>2445)
                     /locus_tag="VHK28_09355"
     CDS             complement(1051..>2445)
                     /locus_tag="VHK28_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX2142459.1"
                     /translation="GELAGCAFADERLGRRLRKLLERLAGAMGESLPLACQDWADTKA
                     AYRFFANDRVSEGQILAGHFHSARQRFAATEGVILVVQDTTEFSFRRERAERVGITCR
                     VNSGKDKAGRSRMHTVCGLLMHASLAVTTEGLSLGLSAVKFWSRQKFKGAAALKRKVN
                     PTRVPIEHKESIRWLENMRQSAQLLGEPARCVHIGDREGDIYELFCTARELGTHFLVR
                     SCVDRLAGNGGHTIADEMNGVPVQGLHRVEVRDGKGRVDTAAVELRYRSVRVLPPVGK
                     QRRYPALTLTVVQAKEQAEPADRPAIDWQLITDLPVSSREAAVEKLRWYAMRWKIEVF
                     HKILKSGCRVEEARLRTAERLVKLIAVLCILSWRVFWLTMIARSAPQATPRLALTGLE
                     MTLLDRLVPDKGRPAPSEKTLAAYLIKVARLGGYLARAKDPPPGNAVMWRGLSRLTDI
                     TLGAALAAPTPACG"
BASE COUNT          364 a          837 c          847 g          397 t
ORIGIN      
        1 gcccggtgga tcatcagcgc ggcctgctgc tcggccgcct tcaccggcac gaagcgcatc
       61 gacggccgcg tcaccgcctc gcagatcgcc gcggcgtcgg cagcgtcgtt cttgccgcgc
      121 ttgacgtagg ccttcacgta atgcggcggc ataagccgca cctcgtggcc gagcgccctg
      181 agctcccgtg cccagtagtg ggcggtcgca caggcttcga tcccgatcac gcaggccggc
      241 agcccggcga agaacgcgag catctgcgaa cgccgcaacc gcttgcgcag cacgacgccg
      301 cccgcctcgt cgacggcatg gacctgaaac acgtgcttgg cgagatcgag gccgatcgtc
      361 gtaatcctca tcgcggacgg ctcccctctc ggtgatctgc ggcgaccacc accttggcac
      421 agcgatgccg tcggggggcc atccacccca tcaaggttgg gtgttcggtc ccggggtgtg
      481 gtggagcggg ttggtgatga agcgctcggg gtcgcgcgtc cactgctggc agacgaaacc
      541 gtagggcgtg aggccgcgca gggccttgag ccgacgagcg tggttgtagg cgtcgacgaa
      601 gagctgcagg tgccggcgga gctgatcgtg gctcccgtag tggtcgcgct tgaccgtggc
      661 gtccttgagg gtgcggttca tccgctcgac ctggccgttc gcccagggat ggttcggcct
      721 ggtcaggcgg tgctcgacgc cgtgctcggc acagatgcga tcgaacaggt gcccccctga
      781 aggtggcggt tggcccgtcg cggtagcgcg gcgggtggca gaactggacg ccgttgtccg
      841 tcagcaccgt gtggatgcgg tagggcacgg cctcgatcag ggtccgcagg aacgcacagg
      901 cggtcctgcg ctcggccttg tcttcaagcc gcgccaaggc gcgcttggag gtgcggtcga
      961 tggcgacgaa gaggacgagc ttgccctcct cggtcctgac ctcaccgatg tcgatgtgga
     1021 agaaacgtaa ccctccggtg aagctttctt ttacccacag gcgggcgtgg gcgccgcgag
     1081 ggcagcaccc aacgtgatgt ccgtgagccg tgacaggccc cgccacatca ctgcgttgcc
     1141 gggcggcgga tccttggcgc gggccaggta gccgccgagc cgggcgacct tgatcagata
     1201 ggcggcgagc gtcttctcgc tcggagccgg ccgccccttg tcaggcacga ggcggtccag
     1261 gagcgtcatc tccaggcccg tcagggcgag ccggggtgtg gcctgcggcg ccgaccgggc
     1321 gatcatcgtc agccagaaca cccgccagct caggatgcac aagaccgcga tcagcttcac
     1381 cagccgctcc gcggtccgga gccgtgcctc ctccacccgg cagcccgact tcaggatctt
     1441 gtggaagacc tcgatcttcc agcgcatcgc gtaccagcgc agcttctcga ccgccgcctc
     1501 acgggaggag acgggcaggt cggtaatcag ctgccagtcg atcgcgggcc ggtcggccgg
     1561 ctccgcctgc tccttggcct gaaccacggt cagggtaagc gccgggtagc gcctctgctt
     1621 gcccacgggc ggaagcacgc gaacgctgcg gtacctgagc tcgacggcag cggtatcgac
     1681 gcgccccttg ccgtcgcgaa cctcgacgcg gtgcaagccc tggacgggca ccccgttcat
     1741 ctcgtcggcg atcgtgtggc cgccgtttcc cgccaagcgg tcgacgcagc ttctgaccag
     1801 gaaatgggtg ccgagctccc gcgccgtgca gaagagctcg tagatatcgc cctcgcggtc
     1861 gccgatgtgg acgcaccgcg ccggctcgcc gagcagctgc gcggactggc gcatgttctc
     1921 cagccagcgg atgctctcct tgtgctcgat cgggacccgc gtcgggttga ccttcctctt
     1981 gagcgcggcg gcgcctttga acttctgccg cgaccagaac ttgacggccg acaggcccaa
     2041 ggacaggccc tcggtcgtga ccgcgaggct ggcgtgcatg agcagcccgc agaccgtgtg
     2101 catcctcgag cggccggcct tgtctttgcc gctgttcacg cggcaggtga tgccgacccg
     2161 ctcggcgcgc tcgcgccgga aggagaactc ggtggtgtcc tggacgacca ggatgacgcc
     2221 ctcggtcgcg gcgaagcgct ggcgcgccga gtggaaatgg ccggcgagga tctgcccctc
     2281 gctgacccga tcgttggcga agaagcggta ggcggccttc gtgtcggccc aatcctggca
     2341 cgccagcggc aggctctcac ccatggcgcc ggccagccgt tcgagcagct tgcgcaggcg
     2401 cctgcccagg cgttcgtccg cgaaagcgca tcccgccagc tcgcc
//
LOCUS       DATACA010000463         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2713303, whole genome shotgun sequence.
ACCESSION   DATACA010000463 DATACA010000000
VERSION     DATACA010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713303"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /gene="glgA"
                     /locus_tag="VHK28_09360"
     CDS             complement(<1..851)
                     /gene="glgA"
                     /locus_tag="VHK28_09360"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX2142460.1"
                     /translation="MANRGAIDPRVLLLTNEFPPSVYGGAGIHVGELAGELRRRIGLD
                     VRTFGDHDERANGWRVRGYRPAHDLAVADERLRPAWAAVSRDVSMAIEPVDADLVHCH
                     TWYVQLAGLLVRKAYGIPLVVTVHSLEPLRPWKREQLGGGYDLSSWIERTALGEAAAV
                     IAVSHGTREDVLRHFPVEPERVRVIHNGIDPDFYAPDPATDTLERYGVDASLPYVLFV
                     GRITRQKGIVHLVRALRHLDPGIGVVLCAGQPDTPEIAREMEEGVAEARRHRSNLVWI
                     PEMVSRT"
     gene            complement(877..1278)
                     /locus_tag="VHK28_09365"
     CDS             complement(877..1278)
                     /locus_tag="VHK28_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2142461.1"
                     /translation="MNQEPHHRGRRGRPPQRAFLVEDDYPTLQLLQDVAASVGLEPVP
                     FSRLSAARRALREGVPEVVVVDDDLPDGQGADLVRELRANPRTRHVRVLFCTAASQDR
                     CRDLGRLAPVINKPFDLTRMERALAEVAASH"
     gene            1439..>1703
                     /locus_tag="VHK28_09370"
     CDS             1439..>1703
                     /locus_tag="VHK28_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX2142462.1"
                     /translation="MTEAAATPTPSFEPAELSLPVVGMTCASCVNRVERFLNRADGVE
                     QAVVNLASERASVRYDPRRIDPAGIAEAIRASGYEVGPEAAAEV"
BASE COUNT          255 a          603 c          599 g          246 t
ORIGIN      
        1 tcggtacggc tcaccatctc cgggatccag accaggttgg accgatgccg ccgagcctcg
       61 gcgactccct cttccatctc gcgcgcgatc tcgggcgtgt ccggctgccc ggcgcacagg
      121 accaccccga tgccggggtc caggtggcgc agcgcacgga cgaggtgcac gatccccttc
      181 tgccgggtga tgcggcccac gaacaggacg tagggcagcg acgcgtcgac gccgtaccgt
      241 tcgagcgtgt cggtggcggg gtccggggcg tagaagtccg gatcgatgcc gttgtggatg
      301 acgcggaccc gctccggctc gaccgggaag tggcgcagca cgtcctcgcg cgtgccgtgg
      361 ctgaccgcga tcaccgccgc cgcctcgccg agcgcggtgc gctcgatcca ggacgacagg
      421 tcgtacccgc cgccgagctg ctcgcgtttc catggccgca acggctccag cgaatgaacg
      481 gtgaccacca gcgggatgcc gtaggccttg cgcaccagga ggccagccag ctggacgtac
      541 caggtgtggc agtggaccag gtcggcgtcc accggctcga tggccattga tacgtcgcgc
      601 gagaccgccg cccaggccgg ccgcagccgc tcgtcggcga ccgcgaggtc atgggccggc
      661 cgatagccac gcacccgcca gccattcgcc cgctcgtcgt ggtcgccgaa ggtccgcacg
      721 tccagcccaa tgcgccggcg cagctcgcct gccagctccc ccacgtggat gccggcaccg
      781 ccgtacacgc tgggcgggaa ctcattggtg agcagaagaa ccctgggatc gatggcgccg
      841 cgattggcca tggcgccgag aagcgtaccc gcagcgtcag tggctcgcag ccacctctgc
      901 cagcgcccgc tccatgcgcg tcaggtcgaa cggcttgttg atcaccggcg ccaggcggcc
      961 gaggtcgcgg cagcggtctt ggctggcggc agtgcagaac agaacgcgga cgtggcgggt
     1021 acgcgggttg gcgcggagct cgcggaccag gtcggcgccc tggccgtccg gcaggtcgtc
     1081 atccacgacc acgacctccg gaacgccctc gcgcagggcg cgacgggcgg cggacagccg
     1141 gctgaacggc accggttcca ggccgacaga ggcggctacg tcctgcagga gctggagcgt
     1201 cggatagtcg tcctcgacca ggaaggcacg ctgaggaggc cgccctcggc ggccgcggtg
     1261 gtgtggttcc tgattcatgg cgtacatccg tccccctggc ggaccggttg ccgtcgaagc
     1321 gaaccgggtt tcgcctggcc taggagcacg atgcatgcca ctggctacct ggttgaagcg
     1381 gcagccgctc gcccgcgttg ttgtcgccta tactcccgga gagtatgctg cgtgcattgt
     1441 gacggaagcc gcagccacac cgaccccctc cttcgaacct gccgagctca gcctgccggt
     1501 ggtcggcatg acctgtgcct cgtgcgtgaa ccgcgtggag cgcttcctga accgtgccga
     1561 cggcgtcgag caggcggtcg tcaacctggc cagcgagcga gcctcggtgc gctacgaccc
     1621 gcggcgcatc gacccagccg gcatcgcgga ggccatccgg gcctccggct acgaggtcgg
     1681 tcccgaggcg gccgccgaag tcg
//
LOCUS       DATACA010000464         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2793528, whole genome shotgun sequence.
ACCESSION   DATACA010000464 DATACA010000000
VERSION     DATACA010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793528"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VHK28_09375"
     CDS             complement(<1..794)
                     /locus_tag="VHK28_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017972926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX2142463.1"
                     /translation="MTLAEVVLSIMFVGLIAYGLFGGADFGAGIWDLLAGGTRRGAAQ
                     RGLIERSIAPIWEANHVWLIFVLVVLWTAFSGAFAAVVTTLYIPLTLAAFGMIARGAA
                     FAFRKSITTLGMRRFLGASFALSSLVTPYFLGAVVGGIALGRVPAGIAEGDVIRSWVN
                     PTSVLGGVLSVLVCAYLAAVFLCADARREDAHELTDQFRIRALATAVLTGLVGIAGLF
                     VLRADAPHLFDGLTGRALPVVMLSVVAGLAALVLLVSRRYVLARAAS"
     gene            complement(791..2122)
                     /locus_tag="VHK28_09380"
     CDS             complement(791..2122)
                     /locus_tag="VHK28_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX2142464.1"
                     /translation="MQMALSLGWHIVFACFGIAFPLLTVFVEWRGHRWGDAELLRLAH
                     TWAKAMAVLFAAGAVSGTLLSFEMGILWPGLMDRFGEVFGFPFVLEGFAFFIEAIFVG
                     VYLFGWNRLSPRAHMLSAVPMIISGMAGAFFVVAANAWMNNPTGFRLDEEGQVVDAQP
                     WAAMFGPSTWPQVTHMLIAAYMVTGFTIASVYAVGMLRGRRERRHRQGLLIPLTFAAV
                     LTPVQIGVGDWAANTVADNQPAKLAAMEGQFETMGGAPLSIGGWFDGEEMRWALEIPN
                     GLSLLVHHDPDGVVLGLEEFPADERPPVNVVHLAFDTMVGIGTALLLLSLWFGWSWWR
                     HRRIPGTPWFLRAVAASGVAAIVAMEAGWVVTEVGRQPWIVYEIMRTAEAVSPAPGLY
                     FGFYAVVAVYILLTALTVFVLRRLAQNSDTAAPQETDARPPDPGAGEVAVR"
     gene            complement(2212..>3346)
                     /locus_tag="VHK28_09385"
     CDS             complement(2212..>3346)
                     /locus_tag="VHK28_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2142465.1"
                     /translation="PVVLLHGVGLTSRYLVPLGRRLAALGYRVLAPDLPGFGRSPKPR
                     GAGWPGAPDVREQTEQLRAWLDACGVERAVLFGNSVGVQVAVEFAARFPERVERLLLE
                     GPTPDPRYRTPLRQYSRVLLNMPFEAPSANALYQVEYLSTGIPRMAQHLVRTVDDPIE
                     LRLPDVPVPALVVRGRHDKTLSQPWAEEFTRLLPNGRLVVVEGAAHNAHCSSPELMAR
                     LTDVFLRGDLDGMTPRPTHPVDVVADDRGPLPRRRLSPLSHAAMDYASAASLIAVPRA
                     LHWGPRTRGLLTTFGILGLADSMMTDYPGGLVQRIPLPVHLNLEASGGLQLVLASLTI
                     LRGEPAAGRWAVAAQGLLEVLRSSLTRVSAGPARVVAVPRTSD"
BASE COUNT          590 a         1169 c         1180 g          407 t
ORIGIN      
        1 gaggcggccc gcgcgagcac gtagcgccgg ctgaccagga ggaccagcgc cgccagcccg
       61 gcgacgacgg agagcatcac caccggcagg gcgcgcccgg tcaggccgtc gaacaggtga
      121 ggggcgtctg ctcgcaggac gaacaggcca gcgatgccca ccaggcccgt caggaccgcg
      181 gtggccagcg ctcggatccg gaactgatcg gtgagctcgt gggcgtcttc ccggcgtgcg
      241 tcagcgcaga ggaacacggc cgccaggtag gcgcagacca gcactgagag cacgcccccg
      301 agtaccgagg tcgggttgac ccagctgcgg atcacgtccc cctcggcgat gcccgccggg
      361 accctgccta acgcgatgcc gcccaccacg gccccgagga agtagggggt gaccagtgag
      421 gacagcgcga aggatgcgcc gaggaagcgg cgcatgccca gggtggtgat gctcttgcgg
      481 aaggcgaacg ccgctccccg ggcgatcatc ccgaacgctg cgagggtcag cgggatgtag
      541 agcgtggtca ctacggcggc gaaggcgccg gagaatgccg tccacagcac caccagcacg
      601 aagatcagcc aaacgtggtt ggcctcccag atcggcgcga tggaccgttc gatgagtccc
      661 cgttgcgccg cgccgcggcg ggtgccaccg gccaggaggt cccagattcc ggcaccgaaa
      721 tcggcgccac cgaagagtcc gtaggcgatc aggccgacga acatgatgct gaggaccacc
      781 tcggccagcg tcaccgcacc gccacctcgc cggcgccggg atctggtggc cgcgcgtcgg
      841 tctcctgcgg tgcggccgtg tcggagttct gggccaggcg ccgcaggacg aacacggtca
      901 gcgcggtcag caggatgtag acggcgacca ctgcgtagaa gccgaagtac agtccgggag
      961 cggggctgac cgcttcggcc gtgcgcatga tctcgtagac gatccacggc tgccgtccca
     1021 cctcggtgac gacccagccc gcctccatcg ccacgatggc ggcgaccccg gaggccgcca
     1081 ccgcccgcag gaaccatggc gtccccggga tacggcggtg ccgccaccag ctccagccga
     1141 accagaggct cagcagcagg agggcggtgc cgatgccgac catcgtgtcg aaggccaggt
     1201 ggaccacgtt gaccggcgga cgttcatcgg cggggaactc ctccaggccc agaacgacgc
     1261 cgtcgggatc gtggtggacg agcagcgaca gaccgttcgg tatctccagc gcccagcgca
     1321 tctcctcgcc gtcgaaccag ccaccgatgg agaggggcgc gcctcccatg gtctcgaact
     1381 gcccctccat cgcggcgagc ttggccggct ggttgtcggc gacggtgttc gccgcccaat
     1441 cccccacgcc gatctgcacc ggcgtcagga ccgccgcgaa cgtcagcggg atgagcagcc
     1501 cctgccggtg acgacgctcg cgccggccac ggagcatccc gacggcgtag accgaagcga
     1561 tggtgaagcc ggtgaccatg taggcggcga tcaacatgtg ggtgacctgg ggccaggtgg
     1621 acgggccgaa catcgccgcc cacggctgcg cgtcgaccac ctgcccctcc tcgtccagtc
     1681 gaaagccggt ggggttgttc atccaggcgt tggccgcgac gacgaagaag gcgcccgcca
     1741 tgcccgagat gatcatcggg acggcgctga gcatgtgggc ccgcggcgag aggcgattcc
     1801 agccgaagag gtagacgccg acgaagatgg cctcgatgaa gaaggcgaat ccctccagca
     1861 cgaaggggaa accgaagacc tcgccgaacc ggtccatcag accgggccac aggatgccca
     1921 tctcgaacga cagcagggtg cccgagaccg ccccggcggc gaacagcacg gccatcgcct
     1981 tggcccaggt gtgcgccagc cgcagcagct cagcatcgcc ccaccggtgc ccgcgccact
     2041 ccacgaacac ggtgagcagc ggaaaggcga tgccgaagca ggcgaagacg atgtgccagc
     2101 ccagggacag cgccatttgc atccgcgccg gatacaggtt ctccgcggca tcggccaaca
     2161 cgtcccacgt ggtcacatga gcctcccaac cattccggag aggtcgcggg gctagtcgga
     2221 cgtccgtggc acggcgacca cccgcgctgg cccagctgat acgcgcgtca gcgatgagcg
     2281 gagtacctcg agaagcccct gcgcggccac tgcccaccgc ccggcggcgg gttcgccgcg
     2341 gaggatggtg agggatgcca gcaccagctg cagtccgccg gaggcctcga ggttgaggtg
     2401 cacgggcagc gggatcctct gcacgagtcc gcctggatag tcggtcatca tgctgtccgc
     2461 cagacccaag atcccgaacg tcgtgaggag tccacgggtc cgggggcccc agtgcagtgc
     2521 acgtggcacg gcgatcagcg aggccgcact cgcgtagtcc atggcggcat ggctgagcgg
     2581 tgacagccgc cgcctgggca gcgggccgcg gtcgtccgcc acgacgtcga ccggatgggt
     2641 ggggcgcggc gtcatcccgt ccaggtcgcc ccggagaaac acgtcggtca gccgcgccat
     2701 cagctcgggc gacgagcagt gagcgttgtg cgcggcgccc tcgacgacga cgagccggcc
     2761 gttgggcagc agccgggtga actcctcggc ccacggttgc gacagggtct tgtcgtggcg
     2821 tccacgcacc accagcgcgg gcaccggcac gtcaggcagg cgcagctcga tggggtcgtc
     2881 gaccgttcgc acgaggtgct gtgccatccg tgggatgccg gtcgagaggt actcgacctg
     2941 gtacaaggca ttcgccgaag gcgcctcgaa cggcatgttg agcaggaccc gggagtactg
     3001 ccgcagcggc gtgcggtacc gagggtcggg cgtcggcccc tccagcagca gccgctcgac
     3061 ccgttccggg aagcgggccg cgaactccac cgcgacctgg acgccgaccg agttgccgaa
     3121 cagcaccgcc cgctcgacgc cgcaggcatc gagccaggca cgcagctgct cggtctgctc
     3181 ccgcacgtcc ggtgcccctg gccaccctgc accgcggggc ttcggggagc ggccgaatcc
     3241 cgggaggtcc ggcgcgagca cgcggtagcc gagcgccgcg aggcgccggc ccagcggcac
     3301 caggtagcgg ctcgtcaaac ccacgccgtg caggagtacg accggc
//
LOCUS       DATACA010000465         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_464362, whole genome shotgun sequence.
ACCESSION   DATACA010000465 DATACA010000000
VERSION     DATACA010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464362"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VHK28_09390"
     CDS             complement(<1..188)
                     /locus_tag="VHK28_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein HicB"
                     /protein_id="HEX2142466.1"
                     /translation="MSKAAFPLKLRTSIKEAAARLAKEDGVSPNQWIAVAVAEKVGAV
                     QTAAEFLKQRAGDAKPEEL"
     gene            complement(545..1666)
                     /locus_tag="VHK28_09395"
     CDS             complement(545..1666)
                     /locus_tag="VHK28_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017275678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2142467.1"
                     /translation="MDEDRSVCVGLDTHKAKIAVAVAEPGRAGEVRFQGEITNRPDAV
                     RRLIERPGARHGRLRIRYEAGPCGDGLHRQITALGHDCTVVAPSLVPRRPGDRVKTNR
                     RDAVTLARLHRAGELTAVWVPDPTHGAMRDLVRARTAAMEAVRRARQQLQGFLLRHDR
                     VFTGRKAWSPAHRRWLAGLRFAHPAQQIVLQEQIDTIEEAERRRDRLDQQIRELVPSW
                     SLAPVVAALQAMRGVAFLSAVVLVAEVGDFRRFANPRQLMAWLGLVPSEHSSGAKVER
                     GGITKAGNSRARRVLVEGAWSYRFPARVTSPIQARLDDVPEAVRAIAWKAQLRLCARF
                     RRLVAAGKNANLVTTAVAREMAAFAWAIACQVQPRGAAA"
BASE COUNT          260 a          679 c          655 g          301 t
ORIGIN      
        1 agctcctccg gcttggcgtc gccggcccgt tgtttcagga actccgccgc cgtctggacg
       61 gcacccacct tttccgccac ggcgaccgcg atccactggt tgggcgagac gccatcctcc
      121 ttggcgagcc gggccgccgc ctccttgatc gaggtccgca gtttcagggg aaaggcggcc
      181 ttgctcatga ccacagtctc ttcaccagcc cgcccggacg gaccactctc atgtcccttt
      241 tccagggcgt gaggcatggg tgttcgtctc ccgatcggct cggcagcaag catttggttg
      301 cgggtcgcgc atggcagggg gcggcgctga agacgacggt cgagcaaact ctgatgcgcg
      361 ggttggctac cgcaggaccc ggttccgtct cggggctgcc tcggtctagg agcggacgtt
      421 ggtgaccagt cccatagtgg tgcacgaggg ttcccctccg tgcaccgccc ccgaccatgc
      481 gcggtccctg ttcgggtgtg gatgcggttg ttctcctcgc tgctggtctt ggtggatgac
      541 gagctcaggc ggcagcaccc ctgggctgga cctggcatgc gatcgcccag gcgaaggccg
      601 ccatctcgcg ggcaaccgcg gtggtgacca ggttcgcgtt cttgccggcg gccacaagcc
      661 gccggaagcg ggcgcagagc cggagctgtg ccttccaggc gatcgcccgc actgcttccg
      721 gcacgtcgtc cagccgggcc tggatcgggc tcgtgacccg cgccgggaag cggtagctcc
      781 aggcaccctc gaccagcacc cggcgtgcgc ggctattccc ggccttggtg atgccgccgc
      841 gctcgacctt ggcaccgctc gagtgctcgg atggcaccag gcccagccag gccatgagct
      901 ggcgggggtt ggcgaagcgg cggaagtccc cgacctcggc aaccagcacc accgccgaca
      961 ggaacgcgac gccgcgcatc gcctgcaggg cggcgaccac gggtgccagc gaccagctcg
     1021 gcaccagttc ccggatctgc tggtccaggc ggtcgcggcg gcgctcggcc tcctcaatgg
     1081 tgtcgatctg ctcctggagc acgatctgct gggcgggatg ggcgaagcgg agcccggcca
     1141 gccagcgccg atgagccggc gaccaagcct tgcggcccgt gaacactcgg tcgtggcgca
     1201 aaaggaagcc ctggagctgc tgcctggccc gtcgtaccgc ctccatggcg gccgtgcgcg
     1261 cccggaccag gtcgcgcatc gccccatgcg tcgggtcggg cacccaaacc gctgtcagct
     1321 cgccggcgcg gtgcagccgg gccagcgtga ccgcatcgcg ccggttcgtc ttcacccggt
     1381 caccgggcct gcgcgggacc agcgagggcg ccaccaccgt gcagtcgtgg ccgagggcgg
     1441 tgatctgccg gtgcaagccg tcgccacacg ggccggcctc gtagcgaatc ctgagcctgc
     1501 cgtgcctcgc gccgggccgc tcgatcagac gccggacggc atcgggccgg ttggtgatct
     1561 cgccttggaa ccggacctcg cccgcgcgtc cgggctcggc cactgccacc gcgatcttgg
     1621 ccttgtgcgt atccagcccg acgcacacgg acctatcctc gtccacggct cgtctcccat
     1681 gtctgaggct cggcgccgga ccgcccggcg caaccctcgt cgaacgacat gatcagagac
     1741 gagccacccc tcgccgagaa agggacatgg ggtctagcag ggccgcgccg cttcgggagg
     1801 atctgttgcg ttggctggct gtaggcgcgc tggcggaccg ctccgcctcg gtcaggcggc
     1861 gaggccgaac actcggtgca cgaaggtccg ctgcg
//
LOCUS       DATACA010000466         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1509166, whole genome shotgun sequence.
ACCESSION   DATACA010000466 DATACA010000000
VERSION     DATACA010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509166"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VHK28_09400"
     CDS             <1..1426
                     /locus_tag="VHK28_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEX2142468.1"
                     /translation="ADIINASWGGYGYSQPLADAFADASDLLVVAAAGNDGSDNDLLP
                     SYPASFDLPNILSVASVHNQGHLSVFTNFGASSVDLSAPGEDILSTLPTGSPAGSYGY
                     GSGTSMAAANASGVAALAASAHPSMVGDGAALRQHLIRTARALPSTRWWVANPSMVDA
                     RAAVVSTPDVRRLSGVDRYATSAAISAASFVPQVPYLFVATGDNFPDALAGGALAAQL
                     GVPLLLVKRTSIPSVVMAEIQRLDPLHIFVLGGTGAVANGVLSLLAPHDDPSSGGPFR
                     LAGVDRYDTAAVISDAGFEDGWGGTVFIANGGNFPDALAGGAASAVVGGPVLLTRSTS
                     LPAPTTSELSRLQPSRIVILGGTGVVSSAVANELKGYAAGGNVVRWSGADRYATSAAT
                     SLNAFPTAGSVFLAAGTTFPDALSGDPSAGAFSAPLLLTARTTLPGAARTELQRLEPV
                     RVFVLGGIAAVAESVANEVKALFP"
     gene            complement(1647..>2189)
                     /locus_tag="VHK28_09405"
     CDS             complement(1647..>2189)
                     /locus_tag="VHK28_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2142469.1"
                     /translation="ILFDGRGYTSPSQVYPHTVITYSYGKTLLAPGQRIGYVTVPPSL
                     PADDRAAMRERIFIQQLAVGYSFPNALLQHALRDLERLSIDVGALERRRDRLVPALRE
                     MGYEASMPEGTFYTMARSPIPDDEAFAELLARHRVLVLPGTIVEVPGWFRISLTASDE
                     MVESGIPRFAAAMEEAKRLT"
BASE COUNT          293 a          793 c          757 g          346 t
ORIGIN      
        1 ggcggacatc atcaacgcct cgtggggcgg atacggctac agccagccac tggccgatgc
       61 ctttgcggat gcctccgacc tcctggtggt ggccgccgcc ggcaacgatg gctcggacaa
      121 cgacctgctg ccgtcgtatc ccgcatcctt tgacctgccc aacatcctgt cggtggcctc
      181 cgtccacaac caggggcacc tgtccgtttt caccaacttc ggggcgtcct cggtcgacct
      241 ctccgctcca ggcgaggaca tcctcagcac cttgcccacc ggctcgcccg ccggctcgta
      301 cgggtacgga tccgggacct caatggcggc ggcgaacgcc agtggcgtgg ccgcgctggc
      361 ggcatccgcc catccgtcca tggtcggcga tggcgcggcg ctccgccagc acctgatccg
      421 caccgctcga gccctgccga gcacgcgctg gtgggtcgcc aacccaagca tggtggatgc
      481 ccgcgcggcg gtcgtcagca cgcccgacgt gcgtcgcctt tccggcgtgg accgatacgc
      541 gacctcggcg gcgatatcgg cggcgagctt cgtgccacag gttccctacc tgttcgtcgc
      601 caccggcgac aacttccccg atgcgctagc cggcggggcg ctggccgccc agctcggcgt
      661 gccgctgctg ctggtcaagc gcacctcgat cccctcggtg gtgatggccg agatccagcg
      721 actcgacccg ctgcacatct tcgtgctggg aggcaccggc gccgtggcca acggcgtgct
      781 cagcctcctg gctccccatg acgatccttc gagtggcggg ccattccgcc ttgccggcgt
      841 ggaccggtac gacacggcgg ccgtgatctc agacgccggc ttcgaggacg gttggggcgg
      901 cacggtgttc atcgccaacg gcggaaactt ccccgatgcg ctggccggcg gagccgcgtc
      961 ggccgtggtc ggcggtccgg tgctgctcac gcgctccacc tcgctgccgg cgccgacgac
     1021 gtcggagctg tcgcgccttc agccctcgcg gatcgtcatc ctgggcggga ccggggtggt
     1081 ttcgtccgcg gtggccaacg agctgaaggg ctacgcggcc gggggcaacg tcgtgcgctg
     1141 gtcgggcgcc gaccgctacg ccacctcggc ggcaacctcc ctcaatgcgt tccccactgc
     1201 gggcagcgtc ttcctggcgg ccggcaccac cttccccgat gcgctgtccg gcgacccgtc
     1261 ggccggcgcc ttctccgccc cgctgctgct gactgctcgc acgaccctgc cgggtgcggc
     1321 ccggactgag ctgcagcgcc tggagccggt tcgcgtcttc gtcctgggag gcatcgccgc
     1381 cgtcgccgag tcggtcgcca acgaggtgaa ggcgctgttc ccgtgaccgg tggagtacat
     1441 gctgatctcg gggatgcgcc gctcgtagcg gattgggccg cgttcacccg gtggccgcaa
     1501 cggccaagaa gcgaggcaga gtcccatgta cctggccccg gcggccaccc ggtgagcgtg
     1561 ccggccatcc acgcggccaa atcggtgcct gcttggccgt tagagccatc ccatgaccac
     1621 ggttcccggc ggccatgccc cgacggtcag gtgaggcgct tggcctcctc catggcggcc
     1681 gcgaagcgcg ggatgccgct ctcgaccatc tcgtcggacg cggtcaggct gatgcggaac
     1741 cagcccggca cctcgacgat cgtgcccggc agcaccagca cccggtggcg ggccagcagc
     1801 tcggcgaacg cctcgtcatc gggaatgggc gagcgggcca tggtgtagaa ggtgccctcc
     1861 ggcatggacg cctcgtagcc catctcgcgg agggcgggca ccagccggtc ccgccggcgc
     1921 tccagggcgc cgacgtcgat cgacagccgc tcgaggtcgc gaagcgcgtg ctgcagcagg
     1981 gcgttcggga aggagtagcc gacggccagc tgctggatga agatccgctc gcgcatcgcg
     2041 gcgcgatcat cggccggcag gctgggcgga acggtcacgt agccgatccg ctggccgggc
     2101 gccagcagcg tcttgccgta cgagtaggtg atcacggtgt gcgggtagac ctgcgacggg
     2161 ctggtgtagc cgcggccgtc gaacaggat
//
LOCUS       DATACA010000467         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_786765, whole genome shotgun sequence.
ACCESSION   DATACA010000467 DATACA010000000
VERSION     DATACA010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786765"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VHK28_09410"
     CDS             <1..401
                     /locus_tag="VHK28_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX2142470.1"
                     /translation="MQALWHMHRLAYRVSGGRAAGDGTLAIHTVGRRSGEPRMTLLNY
                     LDDGGRYVVVASNAGSQRHPAWWLNLQARSGAEVVVGGRRVPVRAHRANGVERERLWE
                     RILEWNPVYAAYAAETARSIPVVVLEPVSR"
     gene            complement(391..1650)
                     /locus_tag="VHK28_09415"
     CDS             complement(391..1650)
                     /locus_tag="VHK28_09415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX2142471.1"
                     /translation="MRIGIVATRLAGVDGVAFEAAKWEMALERMGHTVRLCAGQVDAL
                     RATARLVPPMHFTWPPAERVTASAFDADSDPEAVRAEVERLAELLVPVLDNWVEVQRL
                     DALIVENAWAIPMHLPLGVALRRVVEARGIPAIGHHHDYWWERERFAHCVVPDLLDYA
                     FPPDLPNVRHASINSLAATELRRRRGISSIVVPNVFDFERPRPRPHPAVRRRMRSELG
                     MGEHGLLVVQPTRVVPRKGIELAIELVGRLKDPDAVLLITSPAGDEGLDYLVELERQA
                     EKVGIRLRYAADRFEPDLEGKPIGPAHTLHDAYLAADLITYPSLYEGYGNALIEAIFY
                     GKPVVVNRYSVYVSDIAPLGLKFIEMDGRVTDDLVATVRDALANPARQRRIARHNFEI
                     ARRRLSYKVLRRRLRRLLGEVQGGVTG"
     gene            complement(1699..>2025)
                     /locus_tag="VHK28_09420"
     CDS             complement(1699..>2025)
                     /locus_tag="VHK28_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142472.1"
                     /translation="AWSEANNGWFSGERGGLGSIHTPGPWTLGDVQDWLVGRITDDAP
                     RRDRALRRLEEVAFADGMLPEAYSAEREPDVRIRHWFAWPGAAFGALRLLDQGAALEA
                     RLRANG"
BASE COUNT          306 a          726 c          701 g          292 t
ORIGIN      
        1 ggatgcaggc cctgtggcac atgcatcggc ttgcctatcg agtctccggg ggcagggcgg
       61 ccggcgacgg cacgctcgcc atccacaccg tgggccgtcg cagcggagag ccgcggatga
      121 cgctcctcaa ttacctcgac gatggcggcc gctacgtggt ggtcgcctcg aacgccggca
      181 gtcagcgcca tcccgcatgg tggctcaacc tgcaggccag gtcgggtgcg gaggtggtgg
      241 tcggtggacg ccgagttccg gtacgtgccc accgcgcgaa cggcgtggag cgtgagcggc
      301 tgtgggaacg catcctggag tggaacccgg tctacgctgc ctacgccgcg gagaccgccc
      361 gcagcatccc ggtggtcgtc ctcgagccgg tcagccggtg acgccgccct gcacctcccc
      421 cagcagccgc cgtagtcgcc gccgcagcac cttgtaggac aggcggcggc gcgcaatctc
      481 gaagttgtgc cgcgcgatgc ggcgctggcg cgccggattg gccaacgcgt cgcgcacggt
      541 ggccaccagg tcatcggtca cccgtccgtc catctcgatg aacttcagcc ccagtggcgc
      601 gatgtccgag acgtacaccg agtaccggtt caccaccacc ggcttgccgt agaagatggc
      661 ctcgatcagg gcattgccgt agccctcgta caggctcggg taggtgatca ggtcggcggc
      721 caggtaggcg tcgtgcaggg tgtgggcggg tccaatcggc ttcccttcca ggtccggctc
      781 gaagcggtcc gcggcgtagc gcagccggat gccaaccttc tcggcctgcc gctccagctc
      841 caccaggtag tcgaggccct cgtcgccggc gggcgaggtg atgagcagca ccgcatcggg
      901 gtccttcagc cgccccacca gctcgatcgc cagctctatg cccttgcgcg gcaccacccg
      961 tgtgggttgc accacgagga gcccgtgctc gcccatgccc agctcgctgc gcattcggcg
     1021 gcgcaccgcg gggtgcggcc gcgggcgggg ccgctcgaag tcgaacacat tcggcaccac
     1081 gatcgagctg atcccgcggc gtcgccgcag ctccgttgcc gccagcgaat tgatgctggc
     1141 gtggcgcacg ttcggcaggt cgggcgggaa ggcgtagtcg aggaggtccg gcacgacgca
     1201 gtgcgcgaag cgctctcgct cccaccagta gtcgtggtga tgaccgatgg ccgggatgcc
     1261 acgcgcctcc accacccggc gcagtgcgac tcccagcggc aggtgcatgg ggatggccca
     1321 ggcgttctcc acgatcaggg catccaggcg ctggacctcg acccagttgt cgagcaccgg
     1381 gacgagcagc tctgccaggc gttccacctc ggcgcggacg gcctcgggat cggagtcggc
     1441 gtcaaaggcc gatgccgtca cgcgctcggc aggcggccag gtgaagtgca tgggcggcac
     1501 caggcgcgcc gtggcccgca gggcatccac ctgcccggcg cacaggcgaa cggtgtggcc
     1561 catgcgctcc agggccatct cccacttggc cgcctcgaag gcgacgccgt cgacgcctgc
     1621 cagccgggtg gcaacgattc cgatgcgcat gcagcccgat gctaaccggt catgaccaat
     1681 gggatggcgc ccccgccgtc agccgtttgc ccgcaaacga gcctccaacg cggccccctg
     1741 gtcgagcagc cgtagagcac cgaacgcggc gcccggccag gcgaaccagt gacggatccg
     1801 cacgtcaggc tcgcgctctg ccgagtaggc ctcgggcagc attccatccg cgaaggcgac
     1861 ctcctccagc cgccgcaagg cgcgatcgcg gcggggagcg tcgtcggtga tccgcccgac
     1921 cagccagtcc tggacgtcgc ccagcgtcca tgggccaggc gtgtggatcg agcccagccc
     1981 gccccgctcg ccggagaacc agccgttgtt ggcctccgac caggc
//
LOCUS       DATACA010000468         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2072709, whole genome shotgun sequence.
ACCESSION   DATACA010000468 DATACA010000000
VERSION     DATACA010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2072709"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VHK28_09425"
     CDS             complement(<1..842)
                     /locus_tag="VHK28_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HEX2142473.1"
                     /translation="MEQPHHEQPRHVAAVVLAAGESRRFGSPKQLAELDGRTLLEHVL
                     ERAAGAGLRPMVAVVPVWLTRPAAMDDAEALRWVRNPHPERGMSHSLRLGFAALSPEV
                     EAAVILLGDQPLVPASQLRALLAARGERPLVATRAGGRLSPPVLVERSHFPIVEEPGG
                     DVGLRQVLNDHPEWVVAVEVDEPARDVDTRDDLAALASGSRSVRCPGCGVRLAGDPDG
                     PRHEYIGASSPCWVMYTELGARLAEHLLPGRHVVDAYAAQHPGVDGRRQRQSVALHLI
                     GLCHR"
     gene            866..2470
                     /locus_tag="VHK28_09430"
     CDS             866..2470
                     /locus_tag="VHK28_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2142474.1"
                     /translation="MPSLPETVDLLISGGRLVDGTGAPAREGTLASVDARLHIGPTDW
                     RPSAARELDATDMVVSPGFIDLHSHGGLVVLADPRHEPKVRQGVTSEVIGVDGNSYAP
                     FAHHGDLLDFASLNAGLDGHPEVSFDWDTVASYLDRYDRGQAINVAFMVGNSALRIGA
                     IGWDDEPADERTTANQRAMLREAMEEGAFGVSTGLDYPPGSYASTDELAALTAEAARL
                     GGFYHTHVRYPLGDRFLDPFREAIDIGRGGQGSVHITHFYHRRTYPGSPDEMLALVDG
                     ARADGVDVTFDTYPYEWASTRLLIQLPQWVHSGGPIALKERLADRAVRDRIRAELAER
                     GAAYASAAGWADVRLGAFTRPENLRWESRSVADVMNDTGTDAVDAICDLLLAEDLRVA
                     QVTSGPWTDGLRTFLRHPGAMIGTDSTFLGEKPSPRTYGSYPRVLGQFVREERLLGLE
                     EAVRRMTGAPAARLGLRSRGLLRDGFAADVVVFDASRVRALAAYEEPRQFPEGIEHVV
                     VNGRLVVDGGAHTGAAPGKSLRRGRD"
BASE COUNT          367 a          938 c          916 g          342 t
ORIGIN      
        1 cggtggcaga gcccgatcag gtgcagggcc accgactgcc gctgccggcg cccatccacg
       61 ccggggtgct gcgccgcgta tgcgtcgacc acatgccgtc cgggaagcag gtgctcggcc
      121 aggcgcgcac ccagctcagt gtacatcacc cagcacggcg acgaggcgcc gatgtactcg
      181 tggcgcggcc catccgggtc gccggcgagc cggactccac atccggggca tcgcacgctg
      241 cgagacccgc tggcgagtgc ggcgaggtcg tcacgcgtgt cgacgtcgcg tgccggctcg
      301 tcgacctcga ccgccacgac ccactccggg tggtcgttga gcacctggcg cagccccacg
      361 tcgccgcccg gctcctccac gatcgggaag tggctgcgct ccaccagcac gggcggcgag
      421 aggcggccgc cggcgcgtgt ggcaaccagc gggcgctccc cgcgggccgc gagcagggcg
      481 cggagctgcg aggccggaac cagcggctgg tcgccgagga ggatgaccgc ggcctcgacc
      541 tccggcgaga gcgccgcgaa gcccaaccgc agggagtggc tcatcccacg ctctggatgc
      601 gggttgcgca cccatcgcaa cgcctcggca tcgtccatgg cggcaggacg ggtcagccac
      661 acggggacca ccgcgaccat cggccgcagg ccggcaccgg cggcgcgttc gagcacgtgc
      721 tccagcagcg tcctgccgtc cagctcggcg agctgcttcg gcgagccgaa ccggcgcgac
      781 tcaccggcgg ccaggacgac ggccgcaacg tgccgtggct gttcgtggtg tggctgctcc
      841 atcggtccat atactgcggc cagccatgcc gagcctgccg gagaccgtcg acctgctgat
      901 aagcggcgga cgcctggtgg acggcaccgg cgcgccggca cgcgagggca ccctggccag
      961 cgtcgacgct cgcctgcaca tcggtcccac cgactggcgt ccgagtgcag cgcgcgagct
     1021 ggacgccacc gacatggtcg tcagccccgg tttcatcgac ctccactcgc acggcgggct
     1081 ggtggtgctc gccgacccgc gccacgagcc gaaggtgcgc cagggagtga cctccgaggt
     1141 catcggggtc gacggcaact cgtacgcgcc cttcgcccac cacggcgacc tgctcgactt
     1201 cgccagcctg aacgccggcc tggacggcca tcccgaggtc agcttcgatt gggacacggt
     1261 ggcctcctac ctggaccggt acgaccgcgg ccaggccatc aacgtggcgt tcatggtcgg
     1321 caacagcgcc ctgcgcatcg gtgccatcgg ctgggacgac gagccggccg acgagcggac
     1381 caccgccaac cagcgcgcca tgctgcgcga ggcgatggag gagggggcct ttggcgtgtc
     1441 caccggcctc gactacccgc ccggctccta cgcctctacc gatgagctgg cggcgttgac
     1501 agccgaggcc gcgcgcctcg gcggcttcta ccacacccac gtgcgctacc cgctgggcga
     1561 ccgcttcctg gatccattcc gcgaggcgat cgacatcggg cgcggcggcc agggcagcgt
     1621 gcacatcacg cacttctacc accgccgcac ctacccgggc tcgccggacg agatgctggc
     1681 gctggtcgac ggggcgcgag ccgacggcgt ggacgtcacc ttcgacacct acccctacga
     1741 gtgggcctcg actcgcctac tcatccagct tccccagtgg gtgcattcgg gtggaccgat
     1801 agcgctcaag gagcgcctgg ccgaccgcgc ggtgcgcgac cggatacgcg ccgagctggc
     1861 cgagcgcgga gccgcctacg cctccgccgc gggctgggcg gacgtccgcc tgggggcttt
     1921 cacccgcccg gagaacctgc gctgggagtc ccggtcagtg gcggacgtca tgaacgacac
     1981 cgggaccgat gcggtggacg ccatctgcga cctgctcctg gccgaggacc tgcgcgtggc
     2041 gcaggtcacc agcggcccct ggaccgatgg cctccgcacg ttcctgcgtc accccggtgc
     2101 catgatcggc accgactcca ccttcctcgg ggagaagccg tcaccgcgga cctacggttc
     2161 ctacccgcgc gtgctcggcc agttcgtccg cgaggagcgg ctgctgggac tagaggaggc
     2221 ggtgcgccgc atgacgggcg cgccggccgc acggctgggc ttgcgaagcc gtgggctgct
     2281 gcgggacggg ttcgcggcgg acgtcgtggt attcgatgcc tcgcgagtgc gggcgctggc
     2341 cgcctacgag gagccgaggc agttcccgga gggtatcgag cacgtcgtcg tcaacggcag
     2401 gctcgtggtg gatggcggcg cgcacaccgg cgccgctcct ggcaagtcgc tgcgtcgagg
     2461 gcgggactag cccgcgaggc gagctcgggc gcgcaggaga tcggtgaggg cggacgcctc
     2521 catcggcctg gcgaagtgga agccctggcc gtacttcgcg ccg
//
LOCUS       DATACA010000469         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2714378, whole genome shotgun sequence.
ACCESSION   DATACA010000469 DATACA010000000
VERSION     DATACA010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2714378"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="gltX"
                     /locus_tag="VHK28_09435"
     CDS             <1..1462
                     /gene="gltX"
                     /locus_tag="VHK28_09435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2142475.1"
                     /translation="PSPTGPLHIGTARTSLYNYLFARHVGGTYVLRIEDTDTVRSTVE
                     HEQDIIDNLHWLGISWDEGPQVAGGEDIGPFGPYRQSQRLDRYAAEAQRLLEAGRAYH
                     CWCTSEELDAVRREQERNREAPRYNGRCLRLTDADRAQFEREGRRPAIRFKVPPETIR
                     FDDVIRGEVEFDNALLGDFVIVRADTAPLYHFVVVVDDEAMEISHVIRGEDHLSNTPK
                     HIALIRALGYREPRFGHIPLILNADRSKMSKRKSQTAMSAYREQGYLPEAMVNFLAFL
                     GWSPGTEEEIFTLDQLAERFELEAVHKGGAVFDAARLDYLNGVYIRSLTDEQLALQLR
                     PFLPDALDDASVLRVVPLIKERLVRLSNAPGLVAFLWETDAQVAALYDPEALLAKGRS
                     AAETAAALASARDALAALEDADFAADVLEDRCRATAEELGWKAGDFFRPLRLAITGRS
                     VTPPLFGSMELLGRGPVLARIHAAIGMLSAAGAPTA"
     gene            1459..2181
                     /locus_tag="VHK28_09440"
     CDS             1459..2181
                     /locus_tag="VHK28_09440"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX2142476.1"
                     /translation="MTEFGLGDLLELSLIVPIRSSSGEEVDPSRVPLEGMRHGADEAG
                     RFLPAYTSEENFREFGPPGSDAIQLPARDVFARAESGGSRVIIDPGAPAQLEVPSAVL
                     PFLAAGIDPTTPEAMRARRPYGEPPPLEVPGEVPEPFGSELRRELGELPQVDAAWLLR
                     AGTGWTVGIVLDPDAPLAAFDEVRNRLHAVAGEHLGGRRELAVTDLRAQAVREAYESA
                     AAPWYEKARAKPKGFLGRLFGD"
     gene            complement(2178..>2558)
                     /locus_tag="VHK28_09445"
     CDS             complement(2178..>2558)
                     /locus_tag="VHK28_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2142477.1"
                     /translation="GADVGLLEGDETLVSLGLGGPADVVFDFVGTDDTLDYAARNVAP
                     GGLLSLVGEAGGTLPFGFDRLPVETWVTTTAWGSVADLREVVRLARRGRLRWSVERVP
                     LREAQEAHRRLAGGRVRGRLVLVP"
BASE COUNT          375 a          916 c          919 g          348 t
ORIGIN      
        1 gcccagccca accgggccgc tgcacatcgg gacggcccgt acgagcctct acaactacct
       61 gttcgcgcgc cacgtgggcg gaacctacgt gctgcgcatc gaggacacgg acaccgtacg
      121 cagcacggtc gagcacgagc aggacatcat cgacaacctg cactggctgg gcatcagctg
      181 ggacgagggg ccgcaggtgg ccggcggcga ggacatcggt ccgttcgggc cgtaccgcca
      241 gagccagcgc ctggaccgat acgctgccga ggcccagcgg ctgctggagg cgggccgggc
      301 gtaccactgc tggtgcacgt cggaggagct cgacgcggtc cgccgcgagc aggagcgcaa
      361 ccgggaggcg ccgcgctaca acggccgctg cctgcgcctg accgatgccg accgcgcgca
      421 gttcgagcgc gagggacgcc gcccggcgat ccggttcaag gtgccgccgg agacgatccg
      481 cttcgacgac gtgatccgcg gcgaggtgga gttcgacaac gcgttgctgg gcgatttcgt
      541 catcgtgcgc gccgataccg ccccgctcta ccacttcgtg gtggtggtcg acgacgaggc
      601 gatggagatc agccacgtca tccgcgggga ggaccacctg agcaatacgc cgaagcacat
      661 cgcgctgatc cgcgccctcg gctaccggga gccgcgcttc ggccacattc cgctcatctt
      721 gaacgcggat cgctccaaga tgagcaagcg caagtcgcag acggccatga gcgcataccg
      781 tgagcagggc tacctgccgg aggcgatggt caacttcctg gccttcctgg gctggtcgcc
      841 gggcaccgag gaggagatct tcaccctgga ccagctggcg gagcgattcg agctggaggc
      901 cgtgcacaag ggcggcgcgg tgttcgacgc tgcacgcctg gactacctga acggcgtcta
      961 catccgctca ctgaccgatg agcagctggc gctgcagctg cgccccttcc tgcccgatgc
     1021 cctggatgac gcgtccgtcc tgcgcgtcgt gccgctgatc aaggagcggc tcgttcgcct
     1081 ctctaacgcg ccggggctgg tggccttcct gtgggagacc gacgcccaag tcgccgcgct
     1141 ctacgacccg gaggcgctgc tggccaaggg acgttccgct gccgagacgg ccgcggccct
     1201 ggcttcggcg cgcgacgcgc tggccgccct ggaggacgcg gatttcgcgg cagacgtcct
     1261 ggaggaccgc tgccgcgcca ccgccgagga gctaggctgg aaggcaggtg acttcttccg
     1321 cccgctgcgc ctggccatca ccggacgctc ggtgacgccg ccgctgttcg gttccatgga
     1381 gctcctgggc cgtgggccgg tgctggcccg catccatgcc gcgatcggca tgctgagcgc
     1441 ggccggagcg ccgaccgcat gaccgagttc gggctcggcg acctgctgga gctgagcttg
     1501 atcgtgccca ttcgcagctc ctcgggcgag gaggtggacc catcgcgcgt gccgctggag
     1561 ggcatgcgcc acggggctga cgaggcgggg cgcttcctgc cggcgtacac ctccgaggag
     1621 aacttccgag agttcggccc gcccggctcg gacgccatcc agctcccggc tcgcgacgtg
     1681 ttcgcccggg cggaatccgg tggcagccgc gtgatcatcg accccggggc gccggcgcag
     1741 ctggaggtcc ccagcgccgt gctgcccttc ctggcggcgg ggatcgatcc gaccactccg
     1801 gaggcgatgc gcgcgcggcg gccgtacggc gagccgccac ccctcgaggt gccgggcgag
     1861 gtgcccgagc cgttcggcag cgagctgcgc cgcgagctcg gggagctgcc gcaggtggat
     1921 gccgcctggc tgctgcgcgc cggaacgggc tggacggttg ggatcgtgct cgatccggac
     1981 gcgccgctgg cagccttcga cgaggtgcgc aaccgcctgc acgcggtcgc gggcgagcac
     2041 ctgggcgggc ggcgcgagct ggccgtcact gacctgcgtg cacaggcggt gcgcgaggcg
     2101 tacgagtccg ccgcagcccc gtggtacgag aaagcgcggg ccaagcccaa gggctttctg
     2161 ggccgccttt tcggcgacta ggggaccagc accaaccgcc cgcgtaccct gccgccggcc
     2221 agccgccggt gcgcttcctg cgcctcgcgc agcgggacac gctcgaccga ccagcgcagc
     2281 cgaccacgcc gcgccagccg cacgacctcg cgcaggtcgg ccaccgatcc ccaggcggtg
     2341 gtggtgaccc aggtctcgac cggcagccgg tcgaagccga agggcagcgt gccgcccgcc
     2401 tcaccgacca gcgagagcag gccgccggga gccacgttgc gcgcggcgta gtcgagcgtg
     2461 tcatcggtcc ccacgaagtc gaagacgacg tccgccgggc caccgaggcc caggctgacc
     2521 agcgtctcgt cgccctccag caggccgacg tcggcgcc
//
LOCUS       DATACA010000470         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_305455, whole genome shotgun sequence.
ACCESSION   DATACA010000470 DATACA010000000
VERSION     DATACA010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305455"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..813
                     /locus_tag="VHK28_09450"
     CDS             265..813
                     /locus_tag="VHK28_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX2142478.1"
                     /translation="MSRRLLLALAAASLLLVALAATGAAAAPRNFVTPLNGENEVPDR
                     ETRARGVAIFQLSPDGETMSYKLNASNIENVFMAHIHIQPVGQVIGEDNGPIAVWLFE
                     STMPNDQAPVGGGRHDGVLVSGSFTDDVFVGPLAGMTMDDLVAAIESGRAYVNVHTND
                     GVGETNTGPGDFPGGEIRGQLP"
     gene            942..1250
                     /locus_tag="VHK28_09455"
     CDS             942..1250
                     /locus_tag="VHK28_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2142479.1"
                     /translation="MTRDRAAEAIAFGTGTSQDHKDQIRWVDYSNISWNPVFCKRCDI
                     CVEICPKDTLVLRNDAIIEEDNCILCGLCERYCPDLAIEMIPSAVAAHAAKEGDGNAS
                     "
     gene            1247..2386
                     /locus_tag="VHK28_09460"
     CDS             1247..2386
                     /locus_tag="VHK28_09460"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2142480.1"
                     /translation="MTRKLTQGNEAVFRGAVAAGASYYAGYPISPSTEILNVASGWAA
                     EHPEFRFLQAEDEIASANAVIGASLAGAKAFTATSGPGFSLMQEAIGYAQKVGVPSVF
                     VDVQRVGPATGMPTMPGQGDIMQVKWGSSGDYTPLAFYPNGVEEAFRVTIQAFNAAEE
                     SLSPVVVLSDTFVAHLNEVVDLDELAGTVPVVERQLPPLGGHTRGQPRFFAGVVMYEH
                     GPNAGEPATADADEYMRQYYAAKQRHVEVAQRYALYEHGWNTEASVLVIAYGIVSRVA
                     APIAADYALFRPIRIHPMLTDELRAIADRYEQVVCIEANDGQYADMAELALRREVKRI
                     PLLGGRISLEAVRDGLGRVLSGGPSEPPIPPAPTEPQPRSPLGVG"
BASE COUNT          422 a          854 c          805 g          365 t
ORIGIN      
        1 gtgtccaggg ccgtctcacc gtcggcggcg aacagggcgc ggtggccctc gcgctccacg
       61 tagagacgca gcaggtcgcg gatcttgggg tcgtcgtcga ccagcaggac ggtggccatg
      121 gcgccggaga gtatgaccca cgcatgaagc accgatgaag tggccgctgc ccggtgcgtc
      181 ccggcaccga agtgcaaccc gccgcgtatc aactggctga gctgcccgag cgtgtaaccg
      241 tccccagcag gaggaacgac gcgaatgtcc cggagactgc tcctcgccct tgctgccgct
      301 tcgttgctgc tcgtggccct cgccgccaca ggtgccgcgg ccgctccccg aaacttcgtg
      361 acgccgctga acggcgaaaa cgaagtcccc gatcgcgaaa ctcgtgcacg tggtgtggcc
      421 atcttccagc tcagtcccga tggggagacg atgagctaca aactgaatgc ctcgaacatc
      481 gagaacgtgt tcatggccca cattcacatc cagccagtgg gccaggtcat aggcgaggac
      541 aacggcccca tcgccgtctg gctgttcgag tccacgatgc ccaatgacca ggctccggtc
      601 ggaggcggtc gccacgacgg cgtgctggtc tccggcagct tcaccgacga cgtcttcgtg
      661 gggccgctcg ccggcatgac catggacgac ctggtcgctg ccatcgaatc cggtcgcgcg
      721 tatgtgaacg tccacaccaa cgatggagtg ggcgagacca atactggacc gggagacttc
      781 cccggcggcg agatccgcgg ccagctcccg taaccgctcc cacgggggga agcgttcgag
      841 gagccgcggc cgagtgccgc ggctccgtcg cgctgttcag tggcgatgcg tgctcgcctc
      901 gatcgaactt ctcgggtgga gcggcgtaac atgtgcggcc catgacacgc gaccgagcag
      961 ccgaggccat cgccttcggc acgggcacct cccaggacca caaggaccag atccgctggg
     1021 tcgactacag caacatcagc tggaacccgg tcttttgcaa gcgctgcgac atctgtgtcg
     1081 agatctgtcc caaggacacg ctggtgctgc gcaacgacgc gatcatcgaa gaggacaact
     1141 gcatcctgtg cgggctgtgc gagcgctact gccccgacct ggcgatcgag atgatcccgt
     1201 ccgcggtcgc ggcccacgcc gccaaggagg gggacgggaa cgcctcatga cccgcaagct
     1261 gacgcagggc aacgaggcgg tcttccgggg agcggtggcc gcaggcgcca gctactacgc
     1321 cggctacccc atcagcccct ccacagagat cctcaacgtg gcctcgggct gggccgcgga
     1381 gcacccggag ttccgtttcc tgcaggccga ggacgagatc gccagcgcca acgcggtgat
     1441 cggtgcctca ctggccggcg ccaaggcctt caccgccacc tccggccccg gtttcagcct
     1501 gatgcaggaa gccatcggtt atgcgcagaa ggtgggcgtg cccagcgtct tcgtggacgt
     1561 ccagcgcgtg gggccggcga ccggcatgcc gaccatgccc ggccagggcg acatcatgca
     1621 ggtcaagtgg ggcagcagcg gcgactacac gcccctggcc ttctacccga acggggtgga
     1681 ggaggccttc cgggtgacga tccaggcctt caacgccgcc gaggagtcgc tcagcccggt
     1741 ggtggtgctg tccgacacct tcgtggccca cctcaacgag gtcgtcgacc tcgacgagct
     1801 cgccggaacg gtccccgtcg ttgagcggca gctcccaccg ctgggcggtc acacccgcgg
     1861 ccagccgcgc ttcttcgccg gcgtggtcat gtacgagcac gggccgaacg cgggcgagcc
     1921 ggcaacggcc gacgcggacg agtacatgcg ccagtactac gcggccaagc agcggcacgt
     1981 cgaggtggcg cagcgctacg cgctgtacga gcacggctgg aacaccgagg catcggtttt
     2041 ggtcatcgcc tacggcatcg tcagccgggt ggcggcgccg atcgccgccg actacgccct
     2101 cttccgcccc atccgcatcc accccatgct gaccgatgag ctgcgcgcca tcgccgaccg
     2161 ctacgagcag gtcgtgtgca tcgaggccaa cgacggacag tacgccgaca tggccgagct
     2221 ggccctgcgg cgcgaggtga agcggatccc gctcctgggc ggacgcatct cgctggaggc
     2281 ggtgcgcgac ggcctgggtc gggtgctctc cggcggaccg tccgagccgc ccatcccacc
     2341 tgcgcccacc gagccgcagc cacgctcacc actgggggtc ggctagatgc ccgagacacc
     2401 gctcgccgag cttgacttca agaagaacct caagctcgac ctgttc
//
LOCUS       DATACA010000471         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_305954, whole genome shotgun sequence.
ACCESSION   DATACA010000471 DATACA010000000
VERSION     DATACA010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305954"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHK28_09465"
     CDS             complement(<1..350)
                     /locus_tag="VHK28_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142481.1"
                     /translation="MRTFDELEALWSALPPAPRERGTVRLLCVRREDGAHDTPTEVQI
                     SLEGGVVGDRWAHGWQRRDPERNAQVTLMNASVTEMVAALQADLHLDRRVVRAVLAAH
                     AQEPYRAALTGRRR"
     gene            complement(363..785)
                     /locus_tag="VHK28_09470"
     CDS             complement(363..785)
                     /locus_tag="VHK28_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142482.1"
                     /translation="MQLRLDPATGAVEVHAQDLPAPAAISFGAGSIWVGLYGEHGGPQ
                     AAAGEPTIARISAQDGSVQAMVDIASNSTVEGDLWASDDGVWIRSPNDPFLLRIDPAT
                     DEVGFAAAGFQSGGTVALIDGAVWATSIEFGTVWRLDL"
     gene            928..>2345
                     /locus_tag="VHK28_09475"
     CDS             928..>2345
                     /locus_tag="VHK28_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX2142483.1"
                     /translation="MSSWFDKLLEELQRRQAEQDARREGRPIPREPRMARGRTNGGGK
                     PPPSDDGPTPFPRRGGGGGGQPDVRRWLILGGVAILALFLFGFMGRIVDLVTDLMWYD
                     TLDQRAVLTTRLWSQLGLFFLGLAAFLLPALASIWLARRIAPQVPVRRIGQFEIPDAS
                     RAITWALVAVAAVLALISAGAWSANWEQILLYANSEPFGTADPHFNRDVGFYIFDLPV
                     FRFVQGWAVASLVGILVLTLGAYAAGALRWQFRLTAPVRAHLSILGALLLGLIAVGYQ
                     LDISELVYSRSGYESIQAATYTDMNAQLPAYVILTVVAIAAAALLLLNIWFRTLWALA
                     LAGGAWFVLSVLVGGVYPTFVQNFNVNPNEQNLERPYIADHIASTRAAFELDEIEVRD
                     FSGEGRLSEGVFEGDAPTVDNLRLWDYRPLLTTFGQQQIIYPYYDFLDVDIDRYLIDD
                     EQRQIMLSGRELNIDKLAADART"
BASE COUNT          328 a          853 c          803 g          361 t
ORIGIN      
        1 tgccgccggc gccccgtgag cgcggcacgg tacggctcct gtgcgtgcgc cgcgaggacg
       61 gcgcgcacga cacgccggtc gaggtggaga tcagcctgga gggcggcgac catctcggtc
      121 accgacgcgt tcatcagggt cacctgggcg ttgcgctccg ggtcgcggcg ctgccagccg
      181 tgcgcccatc ggtcacccac cacgccgccc tccaggctga tctgcacctc ggtcggcgtg
      241 tcgtgcgcgc cgtcctcgcg gcgcacgcac aggagccgca ccgtgccgcg ctcacggggc
      301 gccggcggca gggccgacca cagggcttcc agctcgtcga aggttcgcat tccgccggaa
      361 ttctagaggt cgaggcgcca caccgttccg aattcgatgg acgtggccca caccgcgcca
      421 tcgatcaggg ccacggtgcc gccggactgg aaccccgccg cggcgaagcc gacctcgtcg
      481 gtcgcgggat cgatgcggag caggaacgga tcgtttgggc tgcggatcca caccccgtcg
      541 tcggaggccc acaggtcgcc ctccaccgtc gagttgctgg cgatgtcgac catggcctgg
      601 accgaaccat cctgggccga gatccgagcg atggtcggct ctccggccgc cgcctgcggt
      661 ccgccatgct ccccgtagag gccgacccag atgctgccgg ctccgaacga gatcgccgcc
      721 ggggcgggca ggtcctgggc gtgaacctcg accgcgccgg tggccgggtc gaggcgcagc
      781 tgcatcccgt cgtcggtggc cgtggcccac agcgccccgc caccgaaggc cagcccggca
      841 gccgagtggc cgagcgggta ggttgagtga tcggcggcca gcggcgacga aatgccaccc
      901 ggcgccgacc gctaggatgg gcgccacatg tcctcctggt tcgacaagct cctcgaggag
      961 ctgcaacgcc gacaggccga gcaggacgcc cgccgcgagg ggcggccgat cccgcgcgag
     1021 ccgcgcatgg cgcgcggccg cacgaacggc ggcgggaagc ccccgccgag tgacgacggg
     1081 ccgacgccgt tcccgcgtcg cggcggaggc ggtggcggac aaccggacgt gaggcgctgg
     1141 ctgatccttg gcggggtcgc catcctggcc ctgttcctgt tcggcttcat gggccgcatc
     1201 gtggacctgg tcaccgacct gatgtggtac gacacgctcg accagcgggc ggtgctgacc
     1261 acgcgcctgt ggtcgcagct gggcctcttc ttcctgggcc tggccgcgtt ccttctgccg
     1321 gcgctggcca gcatctggct ggcccggcgc atcgcgccgc aggtgcccgt gcgccgcatc
     1381 ggtcagttcg agatcccgga tgcctcccgc gccatcacct gggcgctggt cgcggtggcc
     1441 gcggtgctgg ccctcatctc ggccggcgcc tggagcgcca actgggagca gatcctgctc
     1501 tacgccaact cagagccgtt cggcaccgcc gacccgcact tcaaccggga cgtgggcttc
     1561 tacatctttg acctgcccgt cttccgattc gtgcagggct gggccgtcgc cagcctggtc
     1621 ggcatcctgg tcctcacgct gggcgcctat gccgccgggg cgttgcgctg gcagttccgg
     1681 ctgaccgcgc cggtgcgagc gcacctctcg atcctgggcg cgctgctgct gggcctcatt
     1741 gccgtcggct accagctgga catcagcgag ctcgtgtact cgcgcagcgg atacgagtcc
     1801 atccaggccg ccacctatac cgacatgaac gcccagctgc cggcctacgt gatcctgacg
     1861 gtggtggcga tcgccgccgc cgccctgctg ctgctcaaca tctggttccg gacgctgtgg
     1921 gcgctggcgc tggccggggg cgcctggttc gtgctgtcgg tgctggtcgg aggggtgtac
     1981 cccactttcg tgcagaactt caacgtcaac cccaacgagc agaacctgga gcgaccctac
     2041 atcgcggacc acatcgcctc cacgcgcgcc gcattcgagc tcgacgagat cgaggtccgc
     2101 gacttcagcg gcgagggccg cctcagcgag ggggtcttcg agggtgacgc gccgacggtc
     2161 gataacctgc ggctgtggga ctaccgcccg ctgctcacca ccttcggtca gcagcagatc
     2221 atctacccgt actacgactt cctcgacgtg gacatcgacc gctacctgat cgacgacgag
     2281 cagcgccaga tcatgctcag tggccgggag ctgaacatcg acaagctcgc cgccgacgcg
     2341 cgcac
//
LOCUS       DATACA010000472         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_707427, whole genome shotgun sequence.
ACCESSION   DATACA010000472 DATACA010000000
VERSION     DATACA010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707427"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VHK28_09480"
     CDS             complement(<1..222)
                     /locus_tag="VHK28_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142484.1"
                     /translation="MASPWPHPPLAGTIGQRSRGGGIILARADPMLARIEEAFDRARG
                     GGVIVRRSAGSYSLSSARTGGRIARLRPTG"
     gene            210..1565
                     /locus_tag="VHK28_09485"
     CDS             210..1565
                     /locus_tag="VHK28_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX2142485.1"
                     /translation="MAKPPAPDILVPWMAPFAACFTRPTWQNLLVLVAGAILAPGRRT
                     VTAALSVMGLRHAPTFTNFHRVLNRNRWSSGAVARRLLTLLVGAFVPNGPVVVGIDET
                     LERRWGRKIKARGIYRDPVRSSRGHFVKASGLRWLTGMLLAPIPWAGRVWALPFLTLL
                     APSERFAREHHRRHKRLTDRGRQVLLLVARWLPERRVVAVADSTYAAIELLAAIRSRL
                     TLVTRLRLDARLFEPAPPRRPGTIGRPRVAGARLPTLAERLADPATRWRRVKLGGWYG
                     RSERRLDIVSGTAVWYHTGLPAVPIRYVLVRDAAGAFEPQAFLCTDPGADPLDILRWF
                     VRRWSVEVTFAEVRRHLGMETQRQWSDPAIARATPLLLGLFSLVTLWADGLSATGLVP
                     PRRAAWYPKPALTFSDALAAVRHALWTAETFSTSMDHADVAQIPRATLQRLTQAACFP
                     A"
BASE COUNT          218 a          699 c          587 g          258 t
ORIGIN      
        1 gccggtgggc ctgagccgcg cgatccgccc gccggtgcgc gcgcttgaca gcgagtagct
       61 gcccgccgat cgccgcacga tgacgccgcc accgcgggct cggtcgaacg cctcctcgat
      121 gcgcgccagc atggggtcgg ccctggccag gatgatcccc ccgcctctac tccgttgccc
      181 gatggtccca gccagcggag ggtgcggcca tggcgaagcc accggcaccg gacatcctgg
      241 tcccgtggat ggcgccgttc gccgcctgct tcacccgccc aacgtggcag aacctgctcg
      301 tcctggtcgc cggcgccatc ctcgctcccg ggcggcggac cgtcaccgcg gccctgagcg
      361 tcatgggcct caggcacgcg cctaccttca ccaacttcca ccgcgtgctc aaccggaacc
      421 gctggtcgag cggcgccgta gcccggcgcc tgctcaccct cctggtcggg gccttcgtgc
      481 cgaacggccc ggtggtcgtc ggcatcgacg agacgctcga gcgccgctgg gggcggaaga
      541 tcaaggcccg cggcatctac cgcgacccgg tccgctccag ccgcggccac ttcgtcaagg
      601 ccagcggcct gcgctggctc accgggatgc tgctcgcccc gatcccctgg gccggtcggg
      661 tctgggcgct gccgttcctg accttgctcg cgccatccga gcgcttcgcc cgcgagcacc
      721 accgccggca caagaggctc accgaccggg ggcgccaggt gctcctcctg gtcgcccgct
      781 ggctgcccga gcggcgggtc gtcgcggtgg ccgacagcac ctacgccgcg atcgagttgc
      841 tcgccgccat ccgctcgcgc ctgaccctgg tgacccggct gcgcctggac gcgcgcctgt
      901 tcgagccggc gccgccacgc cgcccgggca cgatcggccg gccgcgggtc gcgggcgcgc
      961 ggctgcccac gctggccgag cgcttggcgg acccggcaac gcgctggcgc cgagtcaagc
     1021 tcggcggctg gtacgggcgc agcgagcgcc ggctggacat cgtctccggc acggccgtct
     1081 ggtaccacac cggcctaccc gcggtgccga tccgctacgt cctggttcgc gacgccgccg
     1141 gcgcgttcga gccgcaggcc ttcctgtgca ccgaccccgg cgccgacccg ctggacatcc
     1201 tgcgctggtt cgtgcggcgc tggtcggtcg aggtgacctt cgccgaggtc cgccgccacc
     1261 tgggtatgga gacccagcgc cagtggtcgg acccggcgat cgcccgggcc acgccgctgc
     1321 tgctcgggct gttctccctg gtcaccctgt gggccgatgg cctgtccgcc accggcctcg
     1381 tcccgccccg ccgggcggcg tggtacccca agccggcgct caccttcagc gacgcgctcg
     1441 ccgccgtccg ccatgcgctg tggaccgcgg agactttctc cacgtcgatg gaccacgccg
     1501 atgtggccca aatcccccgg gccacgctgc aaaggctcac ccaggccgcc tgcttcccgg
     1561 cctgaaatgg ccaaagtcca gctcagagcc cgtccgataa ttgggttcag gcggtcaatg
     1621 cacggacgaa gcgcatgata gagctgagcc tgatcatgct ctcggcgacg tgcggcaggg
     1681 tttcgtgatc gaccagcagg cgtcggtaga accgccccgc cccgaacgct tgctcgaccc
     1741 accatcgctt cgggagcacc ac
//
LOCUS       DATACA010000473         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_65267, whole genome shotgun sequence.
ACCESSION   DATACA010000473 DATACA010000000
VERSION     DATACA010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65267"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2134
                     /locus_tag="VHK28_09490"
     CDS             101..2134
                     /locus_tag="VHK28_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2142486.1"
                     /translation="MSTYRYARWDGSQQVSPFDADDILDAMSEDILAEGDVRRALQRL
                     MQRGLRGTRGGDVPGLRRIAERLRERRSAELERANLDGVLDEIGERLERIVEQERTGI
                     GQRLHEAEQAALDAPPGEEQDRARMAEQVLRRAAQQRRDRLDSLPPGLAGRLHGLRDY
                     EFMDPGARDAFNELTDELRQRLMETYFQGLKDGVAGLTPDDLDGVRQMVRDLNALLEK
                     HASGADTSADFAAFMARHGQYFPPGIGSVDELVDHLHRQASQMASLLSSMSPQMREEL
                     QQLMDDLLRDDRLRWDMARLGGNLQALRPDAPFGEPYPFSGDEPMGLVDALAAIDRQQ
                     QYDAMEQEMLAARDPDALDRLDPELLRDLGGDEAGDELDRLRELARALEEAGYLERDG
                     GRLELTPRAARKLGMRALTDIFSRLRRESLGGHPLPRAGAGGEPTDETKGYEHGDAFV
                     LDLNRTLFNAMARRGPGTPVVLSPADFEVRRTEETTVSSTVLLLDMSRSMLLRGCSTA
                     AKRVAMALHTLIHTKYPRDRLYVVGFAYYARQIKPEAIATLSPYEFEYGTNLQHALMI
                     GRQLLGRRRGGNKEMVVITDGEPTAHIANGQVEFAYPPTIRTMQSTLREVGRATKEGI
                     VINTFMLERSRYLSEFVDLMSRINRGRAFYVEPEHLGEYVLVDYVSKKTKRVA"
     gene            2346..3260
                     /locus_tag="VHK28_09495"
     CDS             2346..3260
                     /locus_tag="VHK28_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2142487.1"
                     /translation="MSRPLKVGVQLPEVEYVARWPEMREMARTAEDIGLDSIWLGDHL
                     LYRDDGRPPRGPWEAWSMLAALSAVTERVQLGPLVASTSFHNPAMLAKKAATIEEISC
                     GRLILGLGAGWNEAEYQAYGFPFDHRVSRFEEAFTIIRQLLSEGRSDFHGTYYALDDC
                     ELLPRGPRPQGPPLMVGSEGPRMLAITLPHVASWNAWFTWFDNGVDGYRSLRDKIDRA
                     CGDAGRDPATLERTVALFVAFPGSQGRAIGNLSRPNADPISGEPQVLAPVLAAFAEEG
                     VGHVQLVVDPITSETVAALRPTLERLDG"
     gene            3297..3689
                     /locus_tag="VHK28_09500"
     CDS             3297..3689
                     /locus_tag="VHK28_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142488.1"
                     /translation="MDELAPPIPLGATGRLEAQPLRNAAGDHALLVRQSGQARALLTG
                     EAGTDRDTVIVVPMDDDVQLRMDGDALAVWLTSGAVTPVSADGAPGGGADLPAWASAI
                     GLLLMLVVVAFAVLGSVTFFGWLLGIVG"
     gene            3689..>3907
                     /locus_tag="VHK28_09505"
     CDS             3689..>3907
                     /locus_tag="VHK28_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142489.1"
                     /translation="MSSERTLPPTSGLIAMRINVEYGSADEFSRTLERALARGGERGA
                     TIVAQLDRGELAIHIPREDGPSWNAVPLV"
BASE COUNT          589 a         1340 c         1406 g          572 t
ORIGIN      
        1 gcggagccgg gcgaccctcc cgccgagccg gtaccactag ctgacctgcc cgaactgacc
       61 gatggacgcg gcggcggcat cggcctaggc tgacttgacc atgagcacct accggtacgc
      121 ccgctgggat ggaagccagc aggtgtcgcc cttcgacgcg gacgacatcc tggatgcgat
      181 gtcggaggac atcctggcgg agggcgacgt gcggcgggca ttgcagcgcc tcatgcagcg
      241 cggcctgcgc ggcacgcgcg gcggcgacgt tcccggcctg cgtcggatcg cggagcggct
      301 gcgggagcgg cgcagcgcgg agctcgagcg cgccaacctg gacggcgtcc tggacgagat
      361 cggggagcgg ctggagcgca tcgtggagca ggagcggacg ggaatcggcc agcggctgca
      421 cgaggccgaa caggcggcgc tcgatgcccc gccgggcgag gagcaggacc gcgcccgcat
      481 ggctgagcag gtgctgcgcc gggcggccca gcagcgccgc gaccggctcg acagcctgcc
      541 ccccggcctg gccgggcgcc tccacgggct gcgggactac gagttcatgg atcccggcgc
      601 acgagatgcc ttcaacgagc tgaccgatga gctccgccag cggctgatgg agacctactt
      661 ccaggggctc aaggacggcg tggccggcct gaccccggac gacctcgatg gggtgcgcca
      721 gatggtgcgc gacctcaacg cgctcctgga gaagcacgcc tcaggggccg ataccagcgc
      781 agacttcgcc gcgttcatgg cgcgccacgg gcagtacttc ccgccgggca tcggcagcgt
      841 ggacgagctg gtcgaccacc tccatcgcca ggcgtcgcag atggcctccc tgctgtcgag
      901 catgagcccc cagatgcgcg aggagctcca gcagctgatg gacgatctgc tgcgcgatga
      961 ccggctgcgg tgggacatgg ccaggctggg cggcaacctc caggccctgc gccccgacgc
     1021 gccgttcggt gagccatatc ccttcagcgg cgacgaaccc atgggcctgg tggatgcgtt
     1081 ggcggcgatc gatcggcagc agcagtacga cgccatggag caggagatgc tggccgcccg
     1141 cgacccggac gcgctcgatc gcctggatcc cgagctgctg cgcgacctgg gcggtgacga
     1201 ggccggcgac gagctggatc ggctccgcga gctggctcgc gcgctcgagg aggccggcta
     1261 cctggagcgc gatggcgggc ggctggagct cacgccgcgc gcggctcgca agctcgggat
     1321 gcgggcgctg accgacatct tcagccgcct gcggcgagaa tccctgggcg gccacccgtt
     1381 gccgcgcgcc ggtgccggcg gcgagccgac cgacgagacc aagggctacg agcacggcga
     1441 cgccttcgtc ctggacctca accgcacgct gttcaacgcc atggcccgcc gcgggccagg
     1501 gacgccggtg gtgctgtcac ctgccgactt cgaggtgcgc cgcacggagg agacgacggt
     1561 ctcctctacc gtcctgctgc tggacatgag ccgttccatg ctgctgcggg gctgctcgac
     1621 cgccgccaag cgggtggcca tggcgctgca caccctgatc cacaccaagt acccgcgcga
     1681 ccggctgtac gtcgtcggct tcgcctacta cgcgcgccag atcaagcctg aggctatcgc
     1741 caccctttcg ccgtacgaat tcgagtacgg caccaacctg cagcacgcgc tgatgatcgg
     1801 ccgccagctg ctcgggcggc ggcgaggcgg gaacaaggag atggtggtca ttaccgacgg
     1861 cgagccgacg gcgcacatcg ccaacgggca ggtcgagttc gcctacccgc ccaccattcg
     1921 aaccatgcag tccacccttc gcgaggtcgg ccgcgccacc aaggagggga tcgtgatcaa
     1981 caccttcatg ctcgagcgct cgcgctactt gtccgagttc gtggatctca tgtcgcgcat
     2041 caaccgcggc cgcgccttct acgtcgagcc cgagcacctg ggcgagtacg tgctcgtcga
     2101 ctacgtgagc aagaagacca agcgcgtcgc ctgaccgtct tcctcagcgc aggtctccac
     2161 gcgggctttc gcggctcgac gtccctccga cggagcggtc ccgtgcgcgt ttgggctcaa
     2221 tcggccctgt ccgcggggcg cgcgccaccg cgaagtgatc ggatcaccgg aacactatta
     2281 cgcggctgct taccggatgc ttatcaggcg cttaccaggg accgtggaaa ggtcacaatc
     2341 ggtccatgag cagaccattg aaggtggggg ttcagctgcc ggaagtggag tacgttgccc
     2401 gctggccgga gatgcgggag atggcgcgga cggcggagga catcggcctg gattcgatct
     2461 ggctgggaga ccacctcctg taccgggacg atggccggcc gccccgtgga ccctgggaag
     2521 cgtggagcat gctggccgct ctgtcggcgg tcactgagcg ggtccagctg gggccgctgg
     2581 tggcatccac cagcttccac aacccggcca tgctggccaa gaaggcggcg accatcgaag
     2641 agatcagctg tgggcggctg atcctgggat tgggtgcagg gtggaatgaa gcggagtacc
     2701 aggcctatgg cttcccgttc gatcaccggg tcagccgctt cgaggaggcc ttcaccatca
     2761 tccgccagct gctgagcgag ggccgcagcg acttccatgg cacctactac gccctggatg
     2821 actgcgagct gctgccgcgc gggccccggc cgcaggggcc gccgctgatg gtgggctccg
     2881 aggggcctcg catgctggcc attaccctgc cccacgttgc gtcctggaac gcgtggttca
     2941 cctggttcga caatggcgtg gacgggtacc gatcgctgcg cgacaagatc gatcgggcct
     3001 gcggcgatgc gggacgcgac ccggcgaccc tggagcgcac ggtggcgctg ttcgtggcct
     3061 tcccgggcag ccaggggcgg gcgatcggga acctgtcccg gccgaatgcc gatcccatct
     3121 ccggcgagcc gcaggtactg gcccccgtgc ttgcggcctt cgcggaggag ggcgtgggcc
     3181 acgtccagct cgtcgtggac ccgatcacgt ccgagaccgt ggccgccctg cgtcccacgc
     3241 tcgagcggct cgacggctga tgggcgggcc cacccgcgct gctatcctcg gcgggcatgg
     3301 atgagctggc accgccgatc cccctggggg ccaccggccg gctggaagcg cagccgctgc
     3361 gaaacgcggc cggcgaccat gcgctgctcg tgcggcagtc gggacaggcc cgcgccctgc
     3421 tgaccggcga ggccgggacc gatcgcgaca cggtgatcgt ggttcccatg gacgacgacg
     3481 tccagctgcg catggatggg gacgccctgg cggtgtggct gacaagcggc gcggtcacgc
     3541 cggtgagcgc ggacggggcg cctggcggcg gcgcggacct gccggcctgg gccagcgcca
     3601 tcggcctgct gctgatgctg gtggtcgtcg ccttcgccgt gctgggcagc gtcaccttct
     3661 tcggctggct gctggggatc gtcggctgat gagcagcgag cgcacgctgc cgcccacctc
     3721 cggcctgatc gccatgcgca tcaatgtcga gtacggctcc gcggacgagt tctcgcgcac
     3781 gctggagcgg gcgctggccc gcggcgggga gcgtggtgcg accatcgtcg cgcagctcga
     3841 ccgcggggag ctggccatcc acatcccgcg cgaggacggt ccctcgtgga atgccgtgcc
     3901 gctcgtc
//
LOCUS       DATACA010000474         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2635435, whole genome shotgun sequence.
ACCESSION   DATACA010000474 DATACA010000000
VERSION     DATACA010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635435"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..450)
                     /locus_tag="VHK28_09510"
                     /pseudo
     CDS             complement(163..450)
                     /locus_tag="VHK28_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611158.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            533..1555
                     /locus_tag="VHK28_09515"
     CDS             533..1555
                     /locus_tag="VHK28_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX2142490.1"
                     /translation="MEMTTIGLDLAKDVLQVHGVDATGEVVLRKRLRRAQVLPFFAAQ
                     PPCLVGMEACTTAHYWAREIAALGHEVRLIPAQYAKAYVKRGKNDAADAEAICEAVGR
                     PSMRFVPVKTVGQQAGLVLHRVRDLLIRQRTMLINALRCHLAEFGIIAPQGPQNVGRL
                     LAVLVGDEADYRLPPLARDVLRLLAEQLASLGARIRAVETRMRVWFRSDATCRRLATI
                     PGIGPIVATALVAAIGDPGVFESGRNFAAWLGLTPRQNSTGGKARLGGITKRGDAYLR
                     RLLLSGAQAVLRWSKGARSDPWLTALSARKPPMVVTVALANKMARIVWAVWTRSTEFR
                     RPALAA"
BASE COUNT          269 a          636 c          675 g          268 t
ORIGIN      
        1 tcgaccgcgc acggcaacct gatgaccgag gacgatcagt ggcgcaagct cgggcgcgtg
       61 gtcgaggtcg cgcgcgaggt ctggggggag gtctgagggg aggcggcgcg caccccacgg
      121 agccggcggc tgatcttccc ccgtttccgg ggacacacgg ggttagctgg ccatgcgctg
      181 ctctgcctgt tcgggcgtgg ggtacccgat ggcggaatgc atgcgggcac gattgtagta
      241 gccctcgatg caggcgaaca ggtcacgccg cgcctcctcc cgcgtcgccc atcgccgctg
      301 gtggaccggt tccacctcca gcgtgtggaa gcagctcccc atcggcgcat ggtcgtagca
      361 gcgggcggtc cggctcatcg aggcggtcgc gccgattgcg gcgagctgat cgccataggc
      421 gccggcggcg tattggctgc cgcggtctga tggggtggat ggcccccgga cggcatctgt
      481 gtgccaaggt ggtggccgtc agaggtcacc acgaacgagg gagccatccg cgatggagat
      541 gacgacgatc ggcctcgacc tggccaagga cgtcctgcag gtccacggcg tcgatgcgac
      601 cggcgaggtc gtcctgagaa agcggctgcg acgcgcacag gtgctcccgt tcttcgccgc
      661 tcagcccccc tgcttggtcg gcatggaggc gtgcaccacg gcccactact gggcccggga
      721 gatcgccgcg ctcggccatg aggtccgcct catccccgcc cagtacgcca aggcctacgt
      781 caagcggggc aagaacgatg ccgccgacgc ggaggcgatc tgcgaggcgg tcggccgccc
      841 gtccatgcgc ttcgtgcccg tcaagaccgt cgggcagcag gccgggctcg tgctccaccg
      901 ggtgcgcgac ctgctcatcc gccagcggac catgctgatc aacgccctgc gctgccatct
      961 ggccgagttc gggattatcg cgccgcaggg cccgcagaac gtcggccggc tgctggccgt
     1021 gctggtgggg gacgaggcgg actatcggct cccgccgctc gcgcgcgacg tccttcgtct
     1081 gctcgcggag caactcgcct cgctcggggc gcggatcagg gccgtcgaga ccaggatgcg
     1141 cgtttggttc aggagcgatg cgacctgccg gcgcctggcc accattccgg gcatcggccc
     1201 gatcgtggcc acggcgctcg tcgcggcgat cggcgacccg ggcgtgttcg agagcggccg
     1261 gaatttcgcc gcctggctcg ggctgacgcc gcgccagaac tcgaccggcg gcaaggcgcg
     1321 gctgggcggc atcaccaaac gaggcgacgc ctacctccgg cgcctgctgc tgagcggggc
     1381 ccaggcggtg ctgcgctggt cgaagggggc gaggagcgat ccgtggctga ccgccctatc
     1441 ggcgcgcaag ccgcccatgg tcgtcaccgt cgcgctggcc aacaagatgg cgcgcatcgt
     1501 ctgggcggtg tggacccggt cgaccgagtt ccgccggccg gctctcgccg cctgagctgt
     1561 ccgccgagcc ggcgggagac aagcttgcga gggcggagga gcatgatggc gtcgtcggtc
     1621 gaccgacgga tcgggaccaa cctggatcat tccctggagc cgcgactccg ccggtctgat
     1681 gaggccccga tccacgaaac ccatcagggc ccgcggccat gtgcgccgca ccgagaggcc
     1741 ggacacatga gcgcaagcga cccggcgcca aacctgacgt tgcccttgca ggaccggggg
     1801 ccatccacac aagagtggtg gatcagcccc ggcgccgggc gctgccgc
//
LOCUS       DATACA010000475         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2475163, whole genome shotgun sequence.
ACCESSION   DATACA010000475 DATACA010000000
VERSION     DATACA010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2475163"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VHK28_09520"
     CDS             <1..199
                     /locus_tag="VHK28_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142491.1"
                     /translation="RTLRILGIWFEPSFGPMEEPDFVPALAEALRAYRSFVGARSVTW
                     PRTRPGRDIAGALRRLAGSAA"
     gene            196..489
                     /locus_tag="VHK28_09525"
     CDS             196..489
                     /locus_tag="VHK28_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142492.1"
                     /translation="MSVWLWALLPAAAWIGGRLWVTRVVVPAWRAGRLSDTPAALMVA
                     ASRAVMLGSAVAAAVMIAALPVAPALVTAALVGLVYAVVGWRSLRAMFRQAER"
     gene            complement(494..1279)
                     /locus_tag="VHK28_09530"
     CDS             complement(494..1279)
                     /locus_tag="VHK28_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX2142493.1"
                     /translation="MRLATWNVNSLKARLERVLAWTERHQPDVLCLQETKLSDEQAPM
                     MDFRALGYELVHHGPGRWNGVAIASRVGIDEVVTGIPTPDGWTDDGGRFLAATCGGLR
                     VATIYVPNGRMVDSEFYLAKLEWLARLARWLDDTDPDTELAICGDYNVAPEEADVWDA
                     AAAHGATHVSPRERAAVAKLREWGMVDIVRRFHPEPGFFTWWDYRAGHFHKNFGMRID
                     HVYVSPRLAARAVAAERDRDARKPSTYPGIPSDHAPLIVDFAD"
     gene            1385..1792
                     /gene="mscL"
                     /locus_tag="VHK28_09535"
     CDS             1385..1792
                     /gene="mscL"
                     /locus_tag="VHK28_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX2142494.1"
                     /translation="MFREFRDFLMRGNVVDLAVAVVIGVAFGAVINSFVADILTPLLG
                     VLGVPDFSELSVTAGEATINYGLFLNAMISFVLIAAAIFFFVVKPMNAIAARRAGQSE
                     EAPTTKACPFCATDIPVGATRCPNCTSELPATT"
     gene            complement(1846..>2216)
                     /locus_tag="VHK28_09540"
     CDS             complement(1846..>2216)
                     /locus_tag="VHK28_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142495.1"
                     /translation="MARQTGAAWLLESWGAWISQPVTVTAAPWICARVPMEASVLRVW
                     ASPQQPTRGGLIDQLIQQIQERTGIERGQAQTAAETAVGFIKERLPDPIAGQVDSVIG
                     DGSGQSADSPMSRVGDMFGG"
BASE COUNT          295 a          783 c          765 g          373 t
ORIGIN      
        1 ccgcaccctg cgcatcctgg gcatctggtt cgagccttcc ttcggaccga tggaggagcc
       61 ggactttgtc cccgccctgg ctgaggccct gcgagcctat cggtccttcg tgggcgcgcg
      121 cagcgtcacg tggccgcgca cgcggccggg ccgggacatc gccggcgcgc tgcgccgcct
      181 ggcgggaagc gccgcgtgag cgtctggctc tgggccctgc tgccggccgc cgcctggatc
      241 ggtggacggc tgtgggtcac gcgcgtggtg gtgcctgcct ggcgcgccgg ccggctgagc
      301 gacacgccgg ccgccctcat ggtcgccgcc tcgcgtgccg tcatgctggg gagcgcggtg
      361 gcggccgcgg tgatgatcgc ggccctgccg gtggctcccg ccctggtgac ggccgcgctg
      421 gtcggcctgg tctatgcggt ggtcggatgg cgctcgctcc gcgccatgtt ccgccaggcc
      481 gaacgctagg cggtcagtcg gcgaagtcca cgatcagcgg agcgtggtcg gatgggatgc
      541 ccgggtaggt ggagggcttg cgggcgtccc ggtcgcgttc tgccgccacc gcacgggcag
      601 ccagccgggg cgagacgtag acgtggtcaa tccgcatgcc gaagttcttg tggaagtggc
      661 cggcccggta gtcccaccag gtgaagaagc cgggctcggg gtggaagcgg cgcacgatgt
      721 ccaccatccc ccactcgcgc agcttcgcca ccgccgcgcg ttcacgcggc gagacgtggg
      781 tggcgccgtg cgcggcggcc gcatcccaga cgtcggcctc ctcgggcgcc acgttgtagt
      841 caccgcagat ggccagctcg gtgtccggat cggtgtcgtc cagccagcgt gccaggcgcg
      901 ccagccactc gagcttggcc aggtagaact cggagtcgac catgcgaccg tttggcacgt
      961 agatggtcgc cacccgcagg ccgccacagg tggcggccag gaatcggccg ccgtcatcgg
     1021 tccagccgtc gggtgtcggg atgccggtga cgacctcgtc gatcccgacc cgtgacgcga
     1081 tggccacccc gttccagcga ccaggcccgt ggtgcaccag ctcgtagccg agagcgcgga
     1141 agtccatcat cggcgcctgc tcatccgaca gcttcgtctc ctgcaggcac aggacgtccg
     1201 gctggtgccg ctccgtccag gccagcaccc gctccaggcg ggccttcagc gagttgacgt
     1261 tccaggtcgc gaggcgcatc ggtccgagtc tagagcggtt gcgcccgcgg gtatgctgcc
     1321 gacgggctct tgcgcagcac ggcagtgagg gctgcctgcg agcgctcacc gtgaggagac
     1381 aggcatgttt cgtgagtttc gcgacttcct gatgcgaggc aacgtggtgg acctcgccgt
     1441 ggcagtggtg atcggcgtcg cgttcggcgc ggtcataaac tcgttcgtcg ccgacatcct
     1501 gaccccgctg ctcggtgtgc tcggcgtgcc ggacttctcc gagctgtcgg tgaccgccgg
     1561 cgaggccacc atcaactacg gcctcttcct gaacgcgatg atcagcttcg tcctgatcgc
     1621 ggcggccatc ttcttcttcg tggtgaagcc aatgaacgcc atcgccgccc gtcgcgccgg
     1681 ccagtctgag gaggcgccca ccaccaaggc ctgcccgttc tgcgcaaccg atatccccgt
     1741 cggcgccacc cgctgcccga actgcactag cgagctgccc gccaccacct gaacaggttc
     1801 gtgcccctcc cccgaggtgg ggaggggcac agggcgggcg atccgctagc cgccgaacat
     1861 atctcccacg cggctcatgg gcgagtcggc agactgaccg gagccgtcgc cgatcacgct
     1921 gtcgacctgg cccgcgatcg ggtcgggtag ccgctccttg atgaagccga ccgcggtctc
     1981 ggccgctgtc tgcgcttgcc cacgctcgat gccggtgcgc tcctgaatct gctggatcag
     2041 ctggtcgatg agtcctcctc gggtcggttg ttgtggacta gcccagacgc gcaagaccga
     2101 tgcctccatg ggcacccgcg cgcatatcca cggggcggct gtaacggtca ctggctggct
     2161 tatccatgcg ccccatgact ccaacagcca cgcagcaccc gtctgcctgg ccatcc
//
LOCUS       DATACA010000476         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_546174, whole genome shotgun sequence.
ACCESSION   DATACA010000476 DATACA010000000
VERSION     DATACA010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546174"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3019)
                     /locus_tag="VHK28_09545"
     CDS             complement(<1..3019)
                     /locus_tag="VHK28_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142496.1"
                     /translation="MTHAPETMTRDSAWRRGASSLGLPRDGVQLVIALIIAGVLLRVL
                     VGGVLLPFSGFRIDVVDFASWAQRLVAVGPGAFYDPGYFSDYPPGYLYVLWLLGSIGQ
                     ALTGLTAGVDITPSLVKIPGVLADAGVAWMLFVYSRRFLGGRIGGWSGERIGLVAAAV
                     YICNPGTIFDSAVWGQVDSVGALVLLASLYWLARGWTEMAAVGAVVAMLVKFQFAFLI
                     PIVAIVGLKRHLLGRSADPELGGRPDQLRVLTSLAAGLGTLVLLTLPFNMPIWAPGEG
                     SLSLISKFIEATNTYDGLTINGFNLWRNPWSGLGDTLAWGCDRPTEGCTAGAGVAFMI
                     GSTIVSWQTIGSLLFALAAGLALWHVARRDEPVGVLQGALLLAVAFFAVPTRVHERYL
                     FPALALAAPLIAQRWRAFAGRPAWIAAALGATVAVLIFVVRPGDPLPADALPGAVLGQ
                     LAVVAVAAGIPLAMAAWGGGALYALLSISFFANVYWVYTADWSFAGGSPMNPGVGGEP
                     MARDPFLAATVFSTWGIFAISFGIVAVLGVLAWRYAREAWVAQPDYATEPMEAREEAP
                     PAAPPVAAQPAEPADRSPSPWQRLGRWMRPDPTDPYLREPYRRLDRLDLAIVLGLVVV
                     ALLFRLWRLDTPRSMHFDEVYHARSATEWLADWQEGWTRDTYEWTHPMLAKYLIAAGI
                     VVADPNKVVDSVQTDAPATAMAISPQRTAHLRPASILFTATGDTTLTARELPSGEVLA
                     EWEAPDPVASLAYDEQNERLLVGSAQDGTVTSYLLAAFTTAGGERAPPVQGTPIESGL
                     ASVEQIVIPQEAALILFRGADGIVAVERVTAAELARSQLVASDIGYLAGADDAVRVVA
                     MDPARNALVALDGATLEPITEVVDGEDVDLVRELPSTPAGVLQVRGRGDDAQVYVPVG
                     QLDASVEHQEVAGGISVFDDTLELIGTAPLPGEPSLIGWQPVANIIYVAGHDSSADQP
                     AVWTIQPIGNGGRQSAGFAAFDTSLLPGDPLAMA"
     gene            3146..>3913
                     /locus_tag="VHK28_09550"
     CDS             3146..>3913
                     /locus_tag="VHK28_09550"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX2142497.1"
                     /translation="MTLVALAIYLRTMMPSAGFWDTAEAQTVPHTLSIFHPTGFPTYA
                     LLGWAWSQIPFGEVAWRMNLLSGVSVALASGLATLTVGQLAPERHRWTMAAAAGVAGL
                     AFAFADEPWQNAVRADVHALHIFVAALLVWLLVTWRSAERAGSPRAGRWLMMAALTFG
                     LGMGNHPLTGLMAPGIALWLFVVDPRFWRRWRLILACALLLAAGMAVYAYIPIRALTP
                     PEPPLFYARPTTLERFRYLVFAEQFRDLFDPFSEPFSN"
BASE COUNT          582 a         1393 c         1396 g          542 t
ORIGIN      
        1 aggccatggc cagcggatcg ccgggcagca ggctggtgtc gaaggcggcg aagcccgccg
       61 actgtcggcc gccgttgccg atgggctgga tggtccacac ggcgggctgg tccgcgctgc
      121 tgtcgtgacc ggcgacgtag atgatgttgg ccaccggctg ccagccgatg agcgacggct
      181 cgccgggcag cggcgcggtg ccgatcagct cgagcgtgtc gtcgaagacg ctgatgccgc
      241 cggcgacctc ctggtgctct actgacgcgt ccagctgccc gaccgggacg tagacctggg
      301 cgtcgtcgcc gcggccgcgg acctgcagga cgccggctgg ggtggacggc agctcgcgca
      361 ccaggtcgac atcctcgccg tccaccacct cggtgatggg ctccagggtg gcgccatcca
      421 gggccaccag cgcgttgcgc gcggggtcca tggcgaccac gcgcacggcg tcatcggccc
      481 cggccaggta gccgatgtcg ctggccacca gctggctgcg agcgagctcc gccgcggtga
      541 cccgctcgac agccacgatc ccgtccgcgc cgcggaacag gatcagcgcc gcctcctgcg
      601 ggatgacgat ctgctccacg ctggccagcc cgctctcgat gggcgtgccc tggacgggcg
      661 gcgcccgctc tccacccgcc gtggtgaacg cggcaagcag gtagctggtg accgtgccgt
      721 cctgggcgga gccgaccagc agccgctcgt tctgctcgtc gtacgccagc gatgcgaccg
      781 gatcgggagc ctcccactcg gccagcacct cgccggaggg aagctcgcgg gcggtcaggg
      841 tggtgtcgcc ggtcgcggtg aacaggatcg acgccgggcg caggtgggca gtgcgttgcg
      901 gggagatggc catggcggtg gccggcgcat cggtctggac ggagtcgacg accttgttgg
      961 ggtcagccac cacgatgccg gccgcgatca ggtacttggc cagcattggg tgcgtccact
     1021 cgtaggtgtc gcgcgtccag ccctcctgcc agtccgccag ccactccgtc gctgagcgcg
     1081 cgtggtagac ctcgtcgaag tgcatggagc gtggcgtatc cagccgccac agccggaaga
     1141 gcagcgcaac cacgaccagc cccagcacga tcgccaggtc gagacggtcg aggcgccggt
     1201 acggctcgcg caggtagggg tcggtcgggt ccgggcgcat ccatcggccc agccgctgcc
     1261 agggggatgg tgaccggtcc gccggctctg cgggttgtgc ggcgacgggt ggcgcggccg
     1321 gcggtgcctc ctctctggcc tccatcggtt cagtcgcgta gtccggctgg gcgacccacg
     1381 cctcgcgcgc gtagcgccag gccaggaccc cgagcaccgc gacgatgccg aaggagatgg
     1441 cgaagatgcc ccacgtgctg aagacggtgg ctgccaggaa cgggtcgcgg gccatgggct
     1501 cgccaccaac acccgggttc attggggatc cgcctgcgaa ggaccagtcc gcggtgtaga
     1561 cccagtacac gttggcgaag aacgagatgg acaggagggc atacagcgcg ccaccgcccc
     1621 aagcggccat ggcgaggggg atgccggcgg ccacggccac taccgccagc tggcccaaaa
     1681 cggccccggg cagggcatcg gccgggagag gatcgccggg gcgcaccacg aagatcagga
     1741 ccgccacggt ggcccccagg gccgccgcga tccacgccgg gcggccggcg aacgcgcgcc
     1801 agcgctgggc gatcagcggc gccgccagcg ccagtgccgg gaacaggtag cgctcgtgca
     1861 cgcgggtggg gaccgcgaag aaggccaccg ccagcagcag cgctccctgc agcacgccga
     1921 ccggctcgtc tcggcgagcc acgtgccaca gcgcgagccc tgcggccagg gcgaacagca
     1981 ggctgccgat ggtctgccag gagacgatgg tcgagccgat catgaaggcc acgccggctc
     2041 cggcggtgca gccctccgtc ggtcggtcgc agccccaggc cagcgtgtcc cccagcccgc
     2101 tccacgggtt gcgccacagg ttgaagccgt tgatggtcag cccgtcgtaa gtgttggtcg
     2161 cctcaatgaa cttgctaatc aggctcaggc ttccctcccc gggtgcccag atgggcatgt
     2221 tgaagggcag cgtcagcagg accagcgtgc ccaggccggc cgccagcgag gtgaggaccc
     2281 gcagctggtc cggccggcca cccagctcgg gatcggccga gcgccccagg aggtgccgct
     2341 tcagccccac gatggccacg atggggatca ggaaggcgaa ctggaacttg accagcatgg
     2401 ccaccaccgc gccaacggcg gccatctccg tccagccgcg cgcaagccag tagagcgagg
     2461 ccagcagcac cagcgcgccc accgagtcga cctgtcccca caccgccgag tcgaagatgg
     2521 tccccgggtt gcagatgtag accgccgccg ccaccaggcc gatgcgctcg ccgctccagc
     2581 cgccgatgcg tccgccgagg aaccggcggc tgtagacgaa cagcatccag gccacgccgg
     2641 cgtccgcgag gacgccgggg atcttcacca gagatggcgt gatgtcgacc ccggcggtga
     2701 gtcccgtcag ggcctgaccg atggagccca gcaaccacag gacgtacagg tatccgggcg
     2761 ggtaatcgct gaagtagccc gggtcgtaga atgcgccggg cccgacggcg accaggcgct
     2821 gtgcccagct ggcgaagtcg acgacgtcga tccggaagcc gctgaacggc agcaggacac
     2881 cgcctaccag gacgcgcagg agcacgccag cgatgatgag agcgatgacc agctggacgc
     2941 cgtcgcgcgg cagaccgagt gagctcgcgc cccgccgcca tgcggagtcg cgggtcatcg
     3001 tttccggggc gtgggtcacg cgctcggaag ggtccggctc ggcaaggtgg cggtgagtat
     3061 acatcgagct ccggacgccc ctggcaggcg gtcggtttcg ctgattccct acgccgcgcc
     3121 cgaggtggcg tggtcggcgg cggccgtgac gctggtcgcg ctggccatct acctgcgcac
     3181 catgatgccc tccgccgggt tctgggacac ggccgaggcc cagaccgtac cccacaccct
     3241 gtccatcttc cacccgaccg gcttcccgac ctacgccctg ctgggctggg catggagcca
     3301 gatcccgttc ggcgaggtcg cctggcggat gaatttgctc tccggggtga gcgtggcgct
     3361 ggcgtccggg ctcgccacct tgacagtcgg ccagctggcc ccggagcgcc atcgctggac
     3421 gatggcggcc gcagcgggcg tggccgggct ggccttcgcc ttcgccgacg agccgtggca
     3481 gaacgccgtg cgcgcggatg tccacgccct gcacatcttc gtggccgccc tgctggtctg
     3541 gctgctggtc acctggcgct cggcagagcg cgccggctcc ccgcgtgccg ggcgctggct
     3601 gatgatggcc gcgctcacct tcgggctggg catgggcaat cacccgctca ccgggctgat
     3661 ggcgccgggc atcgccttat ggctgttcgt ggtcgacccg cgcttctggc gccggtggcg
     3721 cctcatcctg gcctgcgccc tcctgctggc ggccggcatg gcggtgtacg cctacattcc
     3781 catccgggca ctgacgccgc cggagccgcc cctcttctac gcgcgaccca cgaccctcga
     3841 gcgcttccgg tacctcgtct tcgccgagca gttccgggac ctgttcgacc cgttttccga
     3901 gcccttctcc aac
//
LOCUS       DATACA010000477         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1189902, whole genome shotgun sequence.
ACCESSION   DATACA010000477 DATACA010000000
VERSION     DATACA010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189902"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1024
                     /locus_tag="VHK28_09555"
     CDS             257..1024
                     /locus_tag="VHK28_09555"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2142498.1"
                     /translation="MVSRAAALVAEIIDRIEDRRYGTGRPPLPTIEVVETLRFFVREG
                     VQWRELRAAAGRACGSTLRRRLEGWSATALLHQVHVALIRMVRSGPEAASWDVVVDSC
                     SVRAKRGGELTGPNPTHRGKPGTKYHVVVSTDGIPLAAVPSAANVHDTRFFPHLLRLA
                     LAVCAAIGRLYADAAYASADNRRLCRQDGIQPHIREVGEPHGSGLGAVRCVVEHDCAW
                     LLANKRLDRRHDRLGRIILALLTTACIFIIANRLSAF"
     gene            1284..1580
                     /locus_tag="VHK28_09560"
     CDS             1284..1580
                     /locus_tag="VHK28_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142499.1"
                     /translation="MLDQSGSRLEEAMPLFAALLGIPSGERYPGPTLTPEIQKRRTLR
                     ALVDQLAGLAAQPVLALYQDVHWIDPSTLELLGLVIERIRQLRVLVLITFRPEF"
BASE COUNT          249 a          559 c          543 g          258 t
ORIGIN      
        1 cacgaaacgg cggaagctga tggaggactg ggcagcgcac ttggctaagc tagagccggc
       61 caacgtcgtt ccgctggggg ccgcgtcttg agagtaaagc cgaagcgctg gcagtccgcc
      121 aagctgctgt tcgcctaagg agctcggcat ctcaccacgc ttaggggcgg ttttcaaaac
      181 cctgtagctc gtcagcctct cggcatccgg cgtgcctggt gctcgtttcc cgtgcctcgg
      241 gcacggggag aggagcatgg tgtcgagggc ggcggcgttg gtggcggaga tcatcgaccg
      301 gatcgaggac cggcggtacg ggaccgggcg gccgccctta ccgaccatcg aggtcgtcga
      361 gacgctgcgc ttcttcgtcc gcgagggcgt gcagtggcgg gagctgcggg ctgcggccgg
      421 gcgtgcctgc ggctccaccc tgcgccgccg cctggagggc tggagcgcta cggccttgct
      481 gcaccaggtc catgtcgccc tgatccggat ggtgcgctcg ggacctgagg ccgcctcttg
      541 ggacgtcgtg gtcgacagtt gttcggtccg cgccaaacgc ggcggcgagc tgaccggccc
      601 caacccgacg catcggggca agcccgggac caagtaccac gtcgtggtct cgaccgacgg
      661 catcccgctg gccgccgtcc cctcggccgc caacgtccac gacacgcggt tcttcccgca
      721 cctgctgcgc ctggccctgg ccgtctgtgc tgccatcggc aggctctacg ccgacgcggc
      781 ctacgccagc gcggacaatc gcagactctg ccggcaggac ggcatccagc cccacatccg
      841 cgaggtgggc gagccgcacg gctcagggct cggagccgtc cgctgcgtgg tcgagcacga
      901 ctgcgcctgg ttgctggcca acaagcggct ggaccgccgc cacgacaggc tgggccgcat
      961 catcctggcc ctcctgacca cggcgtgcat cttcatcatc gcaaaccgcc tcagcgcgtt
     1021 ctgaaaacca ccccttagat tggcccgccg gctcccttct gaccgcatga tgtggcggcc
     1081 ctggtcgact acggacggaa gcatgagacc ggcgactgga tgtcactcga gggacttgac
     1141 gcccaacggc tcgttacgga agccactact gctcgccgta ccacacgaac agcgccctcc
     1201 accccgtagt cggcctgctg gagcggtccg cgcagctcga ccgcaatgac ctgccggagg
     1261 aagcactggc caagcttgaa gccgtgctcg accagtcggg aagccggctg gaggaggcca
     1321 tgccgctgtt cgctgccctg ctcgggatcc cgtccggcga gcgctacccg gggccgacgc
     1381 tgacgccaga gatacagaaa cggcgaacgc tgcgggcgct ggtcgaccag ctcgcgggct
     1441 tggcggcgca gccggtgctg gctctttacc aggacgtgca ttggatcgac cccagcacat
     1501 tggagctcct gggcctcgtg atcgagcgta tccggcagct gcgggtgctc gtgctgatca
     1561 ccttccgacc ggagttctag ccgccctgga ccgggcaggc gcacgtcac
//
LOCUS       DATACA010000478         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2074697, whole genome shotgun sequence.
ACCESSION   DATACA010000478 DATACA010000000
VERSION     DATACA010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2074697"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..693
                     /locus_tag="VHK28_09565"
     CDS             352..693
                     /locus_tag="VHK28_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142500.1"
                     /translation="MYREWWAKTEACSGLRADFDRVRWSVIEGSSFSCSSGQCAGHWR
                     NDHHIFLASEWVMDEMVVRHEMLHDMIGRPGHPAPPFGEGCPLTWETWNGGASTLTSA
                     LSGALRPTRID"
     gene            complement(690..2057)
                     /locus_tag="VHK28_09570"
     CDS             complement(690..2057)
                     /locus_tag="VHK28_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142501.1"
                     /translation="MLPQAPFTAQLANFWQYFSWQFARDWGRLASLATAVFTVLGLVG
                     LWELWRRDRRAGIAGVALLATLTLGLVFYMNFKYGYSQYPGEPSLPREVRERDYFFVG
                     FFAVYGAFVALGFGATMRGIVEFLGGRGTPGTRWAAATPVLALGLIPLLGNRVTASRA
                     DETIARDFAYDILQSVEPYGILITAGDNDTFPLWYAQEVEGIRPDVTLANLSLMNTRW
                     HLRQLRRRPTPAFDPAEAVALWKPSPEEPGVPLTGTDENRNEPGVWRQPTDPVFSLTE
                     AQLDSLPEAMQVPETGGLAFDSLRIDFGNNVLMLQDLATIFLIRDNLGKRPIHFSWSD
                     GGYPDQTLGLTPYLVSQGFVRKLMPRPVVANDSIVLNPSLGYMDLPRTEKLLWDVYHW
                     KAAARERPRGWVDPPSGSILQLYAVVYGGASKSFAAAGKPALAARADSVADAVVRNLN
                     RDPLF"
BASE COUNT          391 a          734 c          705 g          355 t
ORIGIN      
        1 ctggccgacc cctacatttg cgcatttctg atgtttgaat atcggcatga ctatgtcggc
       61 cgggcggaca tccgggccgc cttagccgag ctccaggagc aagccaagaa gcgtcccaag
      121 cagacctgcc gcaaaccctg acctccgggt cgggcctggc ttcagttctt tccttcctca
      181 gccgatacca gatgcgaccc ccggtcacga cctccagcca cctggtgcgg aagccgctgc
      241 atgctcttag gaagactgtc ccgcagagtt cgtgtgagcc tggccttgat ggtgacggca
      301 ggcgggtgcg gcttcgatcc tcccggtgac gttcccatgg atccaccccc ggtgtaccgg
      361 gagtggtggg ccaagaccga ggcctgctcg gggctccgcg cggatttcga tagagtgcgc
      421 tggtcggtga tcgaaggatc cagcttttcc tgctccagcg gccagtgcgc cgggcactgg
      481 agaaacgatc accacatctt cctggccagt gaatgggtca tggacgagat ggtggttcgc
      541 catgaaatgc tgcacgacat gatcggccgg cccggccatc ctgcgccgcc cttcggcgag
      601 ggctgcccgc tcacctggga aacctggaac gggggcgcct cgacgctcac gtcggctctg
      661 tctggcgccc tccgccccac ccggatcgac taaaacagcg ggtcgcggtt caggttcctc
      721 accacggcat cggcaaccga atcggccctg gccgcaaggg caggtttacc tgccgctgcg
      781 aacgacttcg aagcccctcc gtacaccacc gcatacagtt gcaggatcga tcctgacggc
      841 ggatccaccc agccccgcgg ccgctcgcgt gccgcagcct tccagtggta cacgtcccaa
      901 agcagtttct cggtgcgcgg cagatccatg tagccaaggc tcgggttcag cacgatcgag
      961 tcgtttgcca ccaccggccg gggcatcagt ttccgaacga agccctgcga caccaggtac
     1021 ggtgtgagcc caagcgtctg gtccggatag ccaccgtcgc tccagctgaa atggatcggt
     1081 cgcttcccca ggttgtcacg aatgaggaag atggtggcga ggtcctgcag catgaggaca
     1141 ttgttgccga aatcgatccg caggctgtca aacgccagcc cgccggtctc cggcacctgc
     1201 atcgcttccg gcaggctatc gagctgagct tcggtgagac tgaataccgg atcggtgggc
     1261 tggcgccaaa cgccaggctc gttccggttc tcgtccgtgc cggtgagcgg aacgccgggc
     1321 tcctccggac tcggcttcca caacgccacc gcctcggccg gatcgaacgc gggcgtcggg
     1381 cgccggcgga gctggcgcag atgccaccgg gtgttcatca gcgacaggtt ggccagcgtg
     1441 acgtccggcc gaatgccttc cacctcctgg gcgtaccaga gcgggaaggt gtcattgtcg
     1501 cccgcggtga tcagaatgcc gtagggctca accgactgca gaatgtcgta ggcaaagtcg
     1561 cgagcgatgg tctcgtccgc ccggctggcg gtgacccggt tgcccaggag cggaatcagc
     1621 ccgagggcga gcactggcgt ggccgcagcc cagcgcgtgc caggagttcc ccggccgccc
     1681 aggaactcca caatgccgcg catcgtcgcc ccgaacccca gggccacgaa ggccccatag
     1741 accgcaaaga agcccacgaa gaagtaatcg cgctcgcgca cctcgcgcgg cagggacggc
     1801 tcgcccgggt actgggaata gccgtacttg aaattcatgt aaaagacgag cccgagcgtc
     1861 agcgtggcga gcagggccac tccggcgatg ccggcacgcc ggtcccggcg ccacaactcc
     1921 cacaggccga cgagtcccag aacggtgaac acggccgttg ccagtgaggc cagccgcccc
     1981 cagtcgcggg caaactgcca ggaaaagtac tgccagaagt tggcgagctg ggcggtgaac
     2041 ggcgcctggg gcagcactgg agcgcgcgct cgacgaccga ctgcgcgtct ggacgtccgt
     2101 cgcgggcgac gacggcggtg ccgtggaggt gctctcggcc gggaagctcg catcactccg
     2161 tcgccggtac cacgatctcg gggcg
//
LOCUS       DATACA010000479         5632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1752115, whole genome shotgun sequence.
ACCESSION   DATACA010000479 DATACA010000000
VERSION     DATACA010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752115"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..734)
                     /locus_tag="VHK28_09575"
     CDS             complement(156..734)
                     /locus_tag="VHK28_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2142502.1"
                     /translation="MADIVLFHHVQGLTPGVVAFADELRAAGHTVSTPDLFHGRTFVT
                     LEEGIAHAKTIGFGTLLDRGVAEAAALGTDLVYAGLSMGVMPAQKLAQTSAGARGAVM
                     IDACLPTTEFGDRWPAGVPVQVHGMDSDPIFAGEGDLDAARALVESTPQAELFVYPGD
                     VHLFSDSSLPSHDRAATELMTRRVLDFLSRLS"
     gene            797..1375
                     /locus_tag="VHK28_09580"
     CDS             797..1375
                     /locus_tag="VHK28_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2142503.1"
                     /translation="MASKSQVPDYDLADTMVLSSPSQVQAIGNQLRTTLLGLLHERAA
                     TVTELATAVGRPKSTVAYHINVLERAGLVRVVRTRRVRAIEERYFGRTARMFYVGVED
                     GAGREPLPLDFNDFDVAARESQSAYQAHQLWSFIRHARIPPERAAEFWTQAMALVHEF
                     DGLPRSGNVAYGFAVGIYPIPDYPLLPDAEKE"
     gene            complement(1426..1848)
                     /locus_tag="VHK28_09585"
     CDS             complement(1426..1848)
                     /locus_tag="VHK28_09585"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2142504.1"
                     /translation="MRFEKSIDINAPQQRVWDVLSALEAWPQRIETVEAVELLTPGPI
                     SRGSRVRLKQPKLPEGSWDITVWDAPSYFEWTQKTGGTTSVARHRVEALGEGRSRLTL
                     TIDMRGFLIPIVALFYRKLTERYMDLEAEGMKRAAESA"
     gene            complement(1887..1963)
                     /locus_tag="VHK28_09590"
     tRNA            complement(1887..1963)
                     /locus_tag="VHK28_09590"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Asp,seq:gtc)
     gene            complement(2010..2465)
                     /locus_tag="VHK28_09595"
     CDS             complement(2010..2465)
                     /locus_tag="VHK28_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142505.1"
                     /translation="MGRDTRLAAVVLAALGAVLLIAGVLGLAAGLYLSGWIHDRLPEI
                     TADSAAVGGAAFALGAALVGLAIVHLGLALAVVRTVRAMLLPAAVLSLGMTVAVAGWS
                     VAALVSAAAGAVEPPPMLAAAGILALVAAAYALAARSVIRAWRSTRTRT"
     gene            complement(2481..2996)
                     /locus_tag="VHK28_09600"
     CDS             complement(2481..2996)
                     /locus_tag="VHK28_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142506.1"
                     /translation="MAIRSRGRIAARPTARTARSAARPGRPRAHSLAPAHLGEAASAA
                     LLVLATLGLAVFIAALAMIVFGLTMPGRFGSSPPPNVAELGFGQVFGGIGLLLLGVAM
                     VGSSLAVLAEVRGSRRVAGGVAALAAILSVGGVVATLLQPGQELVLPLALGLAALIFL
                     GVAVILLRPRP"
     gene            3049..4110
                     /locus_tag="VHK28_09605"
     CDS             3049..4110
                     /locus_tag="VHK28_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2142507.1"
                     /translation="MPFELPEHPRSARRAAIWRWVSFALVALLVAALTYLAYIGFVGS
                     DQVSEPPFPSRDCRTPAIAFGWTYEPINYDPTTDVVLEGYPDAANCPEQGVAAGDDVV
                     TSDGKSIAGWYVPAASGIGPEGATLVLAHGHGGNKSSMLGHAEVLHDDYNLVLFDLRN
                     HGQSEDAPTTVGVHERRDLQAMVDWLETNKAPRALGVLGTSMGSAVAVNEAMNDSRVD
                     ALVLESTHATLANALEARLRRAGYPLAVPAAWSILMGGLIRTGADMSAADPVQAIGRL
                     EEVPVLLIGGGQDYSMGPTDAEDLQAAAQAAGIDAELHVCEPAGHGGAHIGCPDEYRD
                     WVLGFLARTLDRAASAPGG"
     gene            4148..>5632
                     /locus_tag="VHK28_09610"
     CDS             4148..>5632
                     /locus_tag="VHK28_09610"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX2142508.1"
                     /translation="MLPTTRQSAISDADARFDRTVDRWFRELLALDPTTATYLGIHEH
                     DQRLADGGREEVQALAAFHRATIAEMERFDASDLTADRALDRDLVVHESRLAAYQLTE
                     RRQWSGSSRAAEHIGEALFPLFTRDFAPLEDRLGSIAARLEDAPRYMAASRGRVDAPV
                     QLWLEIDLESTETLPQFLDSIMAAAGAERVASDVFGRLERAVHGARQALAEHAEWIRR
                     DALPGATAEWRAGPEMFEELIRLRALGGSGDEILAVGEEILATERAARDEVAAEIDPT
                     LSPQEVADIVKDDHPATFPQALEAYRESMNRARAFVVDHGLATLPPQDRLRVIETPIF
                     QRHLVPFAAYYDPPRFDPDPSGTYIVTPPGTPEMWREHNYASISNTSVHEAYPGHHLQ
                     LTAATTNPSLVRTLGLSAAEFVEGWAFYCERMMKDAGFDAQPTHRYIQHTDAIWRAAR
                     IILDVRLHRGDIGFDDAVDFLVEHTGFERHGALAEVRRYTSTPTY"
BASE COUNT          889 a         1986 c         1975 g          782 t
ORIGIN      
        1 tggccggcta tcgccgagcc gagctccagg acgctggatg ccgtcggagt cttcgtgctg
       61 atcccgttgg gcgggatcat cgcggccgtc tacctggcgg ccgttctcgt gcgacgccgc
      121 agggacggct gagccggccg ccgccccacg acgcctcagg agagccggga caggaagtcc
      181 aggacgcgac gggtcatcag ctcggttgcc gcccggtcgt gggacggcag ggaggaatcg
      241 gagaacaggt ggacgtcgcc agggtagaca aagagttcgg cctgaggggt cgattctacg
      301 agggcacgag cggcgtcgag atcgccctcg cccgcgaaga tcgggtcgga atccatgcca
      361 tggacctgga ccggcacgcc tgcaggccat cgatcaccaa actccgtcgt cggcagacag
      421 gcatcgatca tcaccgcccc gcgggcgcct gcgctggtct gggcaagctt ctgtgcgggc
      481 atgactccca tcgagagccc ggcgtacacc aggtccgtcc ccagggctgc cgcctccgcg
      541 acgccgcgat ccagcagggt tccgaagccg atcgtcttgg cgtgggcgat gccctcttcc
      601 agggtcacaa aggtccggcc gtggaagagg tcgggcgtgc tgaccgtatg gccggccgcc
      661 cgcagctcgt cagcgaacgc cacaacgccg ggagtgaggc cctgaacgtg atggaaaagg
      721 acgatgtcgg ccatgggtct ccccttcgca ttgatccaaa attcttgaat gttctgggat
      781 gatagtctaa taagccatgg ccagcaagtc gcaggtgccg gactacgacc tcgcggacac
      841 gatggtgctc agctcccctt cgcaggtgca ggcgatcggc aaccagctcc ggaccacgtt
      901 gctggggctc ctgcacgaac gggcggccac cgtgacggag ctggcgaccg cggtgggccg
      961 gccgaagagc acagtcgcct accacatcaa cgtgctcgaa cgggcgggcc tcgtccgtgt
     1021 cgttcgtacg cgccgggtgc gcgcaatcga ggagcgctac ttcggccgca ccgcgcgaat
     1081 gttctatgtt ggcgtcgaag atggcgcggg ccgggagccg ctgccccttg atttcaacga
     1141 cttcgacgtg gcagccaggg agtcgcagtc tgcctaccag gcgcaccagc tgtggtcgtt
     1201 cattcgacac gcccgcatcc cgccggagcg agcggccgag ttctggacgc aggcgatggc
     1261 cctggtccac gagttcgacg gcctgccgcg atcagggaac gtcgcctacg gatttgcggt
     1321 gggcatatat ccgatcccgg actacccgct gctgccggat gcggagaagg agtaggggaa
     1381 cacccgaggt accgcccggg acgatctcca gtctgcggcc gttgctcagg ccgattcggc
     1441 tgcccgcttc atgccctcgg cctctaggtc catgtagcgc tcggtcagct tcctgtagaa
     1501 cagggcgacg atcgggatga ggaagccgcg catgtcgatg gtcagcgtga ggcgtgacct
     1561 gccctctccc agagcctcga cgcggtggcg ggccacactg gtggtgccgc ccgtcttctg
     1621 cgtccactcg aagtaggacg gcgcatccca gaccgtgatg tcccagctgc cctcgggcag
     1681 cttcggctgc ttgagccgca cgcggcttcc tctgctgatc gggcccggcg tcaggagctc
     1741 gaccgcctcg acggtctcga tgcgctgtgg ccaggcctcg agggcgctga gcacgtccca
     1801 gacccgctgc tggggagcgt tgatgtcgat cgacttctcg aagcgcatgt cgggcggtca
     1861 actgccaggt gatgggtgca gtctgatggc gggcccgacc ggattcgaac cggcgatctc
     1921 cagcgtgaca ggctggcatg ttgggccact acaccacggg cccgcgggag tgacggcggg
     1981 cgcgagtata acaaggatcg ggagggcctt caggtccggg tcctggtgct cctccaggcc
     2041 cgaatgacgc tgcgcgcggc caacgcgtag gcggcggcca ccagggccag gatgcccgcc
     2101 gcggcaagca tcggcggcgg ctcgacagca cctgccgcgg cgctgacgag cgctgcaacc
     2161 gaccaacccg ccactgccac ggtcatgccc aggctgagca ccgccgctgg gagcagcatg
     2221 gcgcgcaccg tccggacgac ggccagcgcc aggcccaggt gcacgatcgc cagcccgacc
     2281 agcgcggcgc ccagggcgaa ggcggcgccg cccaccgccg ccgagtcggc cgtgatctcc
     2341 ggcagccggt cgtggatcca ccccgacagg tacaggccgg cggccagccc cagcacgccg
     2401 gcgatcagca ggacggcacc cagggccgcc agtaccacag ccgccaggcg ggtgtctcgg
     2461 cccacgcggc cggtggggcc ctacggccgc gggcggagga ggatgacggc gacgcccagg
     2521 aagatcaggg ccgccaggcc cagcgccagg ggcaggacca gctcctgccc gggctgcagc
     2581 agggtggcga ccacgccgcc gaccgagagg atggccgcca gggcggcaac gccaccggcc
     2641 acgcggcggc tcccacgtac ctcggccagc accgccagcg atgagccgac catggccacc
     2701 cccagcagca acaggccgat gccgccgaac acctggccga atcccagttc cgccacgttc
     2761 ggcggcggcg aggagccgaa gcgaccgggc attgtcaggc cgaaaacgat catggccaac
     2821 gccgcgatga acacggccag ccccagggtc gccagcacga gcagtgcggc cgaggcggcc
     2881 tctcccaggt gggcgggcgc cagcgagtgc gcgcgcgggc gacccggacg ggcggcgctg
     2941 cgggcggtgc gggctgtcgg gcgtgcggcg atacggccgc ggctgcgaat ggccattccg
     3001 cgggattgta ggtgacggtg ccgcggagcg cgaacgtata ctccggccgt gccgttcgag
     3061 ctgcccgagc atccacgttc ggccaggcgt gccgccatct ggcgctgggt ctcgttcgcc
     3121 ctcgtcgcgc tgctggtagc ggcgctgacc tacctcgcct acatcggctt cgtcggctcc
     3181 gaccaggtct ccgagccgcc gttccccagc cgcgactgcc ggacgccggc catcgccttc
     3241 ggctggacgt acgagcccat caactacgac ccaaccacgg acgtggtcct ggagggctac
     3301 ccggacgcgg ccaactgccc ggagcagggc gtcgcggcgg gtgacgacgt cgtcacctcg
     3361 gatgggaaga gtatcgccgg gtggtacgtg ccggccgcgt ccggcatcgg tccggagggc
     3421 gcgaccctgg tgctggcgca cggccacggc gggaacaaga gcagcatgct gggccacgcc
     3481 gaggtgctgc acgacgatta caacctggtc ctgttcgacc tgcggaacca tggccagagc
     3541 gaggatgccc cgaccacggt gggcgtccac gagcgccggg acctgcaggc gatggtcgac
     3601 tggctggaga ccaacaaggc cccgcgggcg ctcggcgtgc tgggcacgtc aatgggcagc
     3661 gcggtcgcgg tcaacgaagc catgaacgac tcgcgcgtcg acgccctggt gctcgagtcg
     3721 acgcacgcca ccctggccaa cgccctcgag gcgcgcctcc ggcgggccgg ctacccgctt
     3781 gccgtgcctg ccgcctggtc gatcctgatg ggcgggctga tccggaccgg cgccgacatg
     3841 agtgccgccg acccggtgca ggccatcggc cgtctggagg aggtgccggt gctgctcatc
     3901 ggtggcggcc aggactattc gatgggaccg accgacgcgg aggacctgca agccgcggcg
     3961 caggcggccg gcatcgatgc cgagctccac gtctgcgagc ctgcagggca cggtggtgcc
     4021 cacatcggct gcccggacga gtatcgggac tgggtgctag gcttcctggc gcgcaccctt
     4081 gaccgcgctg ccagcgctcc cggcggctga gggccacacg gggctcaccc tctccctcgg
     4141 aggaccgatg ctgcccacca cccgacaatc cgccatctcc gacgccgacg cgcgcttcga
     4201 ccgtaccgtg gaccgctggt tccgtgagct gctggcactg gatcccacca cggccaccta
     4261 ccttggcatc cacgagcacg accagcggct ggcggacggt ggccgggagg aggtgcaggc
     4321 gctcgccgca tttcatcggg cgacgatcgc cgagatggag cgcttcgacg catcggacct
     4381 gaccgccgat cgggcgctcg accgcgacct ggtggtgcac gagtcgcggc tggccgccta
     4441 ccagctgacg gagcggcgcc agtggtcggg gagctcgcgc gccgcggagc acatcggcga
     4501 ggccctcttc cctctcttca cgcgcgactt cgcgcccctc gaggatcgcc tgggatccat
     4561 cgccgcccgg ctggaggacg cgccgcgcta catggccgcc agccggggcc gggtggatgc
     4621 cccggtgcag ctctggctgg agatcgacct ggagagcacc gagacgctgc cccagttcct
     4681 ggactcgatc atggccgccg ccggcgccga gcgggtggcg tccgacgtct tcggccgtct
     4741 cgagcgggcg gtgcacggtg cccgacaggc gctggccgag cacgccgagt ggatcaggcg
     4801 cgacgcgctg cccggcgcga cggccgagtg gcgtgccggg ccggagatgt tcgaggagct
     4861 catccggctg cgggcactgg gtggcagcgg cgacgagatc ctggccgtcg gcgaggagat
     4921 cctggccacg gagcgcgcgg cgcgcgacga ggtggccgcc gagatcgacc cgaccctgtc
     4981 cccccaggaa gtggccgaca tcgtcaagga cgaccacccg gccaccttcc cgcaagcgct
     5041 ggaggcgtat cgagagtcga tgaatcgcgc ccgcgccttc gtggtcgacc acggcctggc
     5101 cacgctgccg ccgcaggacc ggctgcgggt gatcgagacc cccatcttcc agcgccacct
     5161 ggttccgttc gccgcctact acgatccgcc gcgcttcgac ccggacccct cgggcaccta
     5221 catcgtcacc ccgcccggaa cgccggagat gtggcgcgag cacaactatg catcgatcag
     5281 caacaccagc gtccacgagg cgtacccggg ccaccacctg cagctgaccg ccgccaccac
     5341 caatcccagc ctggtgcgca cgctggggct gagcgccgcc gagttcgtgg agggctgggc
     5401 cttctactgc gagcggatga tgaaggacgc cggcttcgat gcgcagccga cgcatcggta
     5461 catccagcac accgacgcca tctggcgggc agcgcgcatc atcctggacg tgcggctgca
     5521 ccgcggcgat atcggcttcg acgacgcggt cgacttcctg gtcgagcaca ccggcttcga
     5581 gcgccacggc gccctggccg aggtcaggcg ctacaccagc acgcccacct ac
//
LOCUS       DATACA010000480         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1190162, whole genome shotgun sequence.
ACCESSION   DATACA010000480 DATACA010000000
VERSION     DATACA010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190162"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1579
                     /locus_tag="VHK28_09615"
     CDS             326..1579
                     /locus_tag="VHK28_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2142509.1"
                     /translation="MTAPSSIDPARFLHEHLASASPDLLRSMLTTFVNTLMSAEADAV
                     CGAAYGESSPERTNVRNGYRNREFDTRAGTLDVAIPKLRQGSYFPDWLLERRRRAERA
                     LTTVVATCYLLGVSTRRMEKLVESLGITRLSKSQVSTMAAELDAHVADFRTRPLDQGP
                     YTFVAADALVLKVREGGRVVNVHALLATGVNADGHREILGLQVTSAEDGAGWLGFFRD
                     LTARGLTGVRLVTSDAHRGLVEAIGATLPGAAWQRCRTHYAANLMAATPKASWPWVRA
                     LLHSVYDQPDATSVHAQFDRVLDAVADKLPKVAEHLDTARPDVLAFTGFPKELWRQIW
                     SNNPSERLNREIRRRTDVVGIFPDRDALIRLVGAVLAEQHDEWAEGRRYLGLDVLTRA
                     RLTPVSDTTTGEEVTTTDTIPALSA"
     gene            1637..2092
                     /locus_tag="VHK28_09620"
                     /pseudo
     CDS             1637..2092
                     /locus_tag="VHK28_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          349 a          804 c          694 g          324 t
ORIGIN      
        1 ggggtgtgcc cgggatggtc ggcggcgtga acgagcggcc atagccgcgc gtcccagtca
       61 gtcggccgcg cccgcggtgg tcgttcacaa ggttgcctca cgattccgtc ggcactgtca
      121 ccggccagga cggttccttc ccggccggcg acggggtggc gagtgacgaa ctgagtgaca
      181 acgaccgcgc ccacaggagt acgggcaagt ggtgtccgcc agggtggtgt gcgaccagcc
      241 cggtgacggg cggagcctcg tgtagggcgg ccatcgcgtg atcttctgga gacctctcac
      301 agtgcttcag aaggacgacc acgcgatgac cgcaccgagc agtatcgacc ctgcccgttt
      361 cctgcacgag cacctggcct cggcctcgcc ggacctgctg cgctcgatgc tgaccacctt
      421 cgtcaacacc ctgatgtcgg ccgaggccga cgccgtctgc ggcgctgctt atggcgaatc
      481 gagccccgag cggaccaacg tgcgcaacgg ctaccggaac cgcgagttcg acacccgcgc
      541 cggcaccctg gacgtggcga tccccaagct gcgccagggc agctactttc ccgactggtt
      601 gctcgagcgc cgccgccgcg ccgaacgcgc cctgaccacg gtggttgcca cctgctatct
      661 gctcggcgtg tccacccggc ggatggagaa gctcgtcgaa tcgctcggga tcacccggct
      721 gtcgaagtcc caggtgtcca ccatggccgc cgagctcgac gcccacgtcg cggacttccg
      781 cacccgcccc ctcgaccagg gcccctacac cttcgtcgcc gccgacgccc tggtgttgaa
      841 agtccgtgag ggcgggcggg tggtcaacgt gcacgccctg ctcgccaccg gggtcaacgc
      901 cgacgggcat cgggagatcc tcggcttgca agtcaccagc gccgaggacg gcgccggctg
      961 gctcgggttc ttccgggacc tgaccgcccg cggcctgacc ggcgtccggc tcgtcacctc
     1021 cgatgcgcac cgcggcctgg tcgaagcgat cggcgcgacc ttgcctggcg cggcctggca
     1081 acggtgcagg acgcactacg cggcgaacct gatggcagcg accccgaagg cgtcctggcc
     1141 gtgggtgcgg gcgctgctgc actcggtcta tgaccagccc gacgccacct cggtgcacgc
     1201 ccagttcgac cgcgtcctcg acgccgtggc cgacaagctc cccaaggtcg ccgagcacct
     1261 cgacaccgcc cgcccggacg tgctcgcctt caccgggttc ccgaaggagc tgtggcgcca
     1321 gatctggtcg aacaatccca gcgagcggct gaatcgcgag attcgccgcc gcactgacgt
     1381 tgtcgggatc ttccccgacc gcgacgcgct gatccgcctg gtcggcgccg tgctggccga
     1441 gcaacacgac gaatgggccg aaggccgccg ctacctcggc ctcgacgtcc tgacccgcgc
     1501 ccgcctcacc cctgtatccg acaccaccac cggtgaggag gtgaccacga ccgacaccat
     1561 tccggcgctc agcgcctgac caagcaggaa gatcacgcgc cagccgtgaa acaccacgcc
     1621 agcggacttg accgtgctgc gggctcatgt agagcggttc ccgccgctga cgatgaccgt
     1681 tccgtggcgc gacccggatg gcgaaccaac cagcgtccgt gtgctcacct ggacccgcga
     1741 gcgcggagcg ctcaaccgga actcgttcaa ccatcacgtc tggaagccgg ccctgcgtaa
     1801 ggctggcatc acgacaccga agcgcgccga cgggttccat gctctgcgcc atttctacgc
     1861 ctcggtgctc ctcgacggcg gggagtcgat caaagctctg tccgagtacc tcgggcacgc
     1921 cgatcccggc ttcaccctgc gcacttacac ccacctcatg ccttccagcg ccgagcgcac
     1981 gcgacgggcc gtggacgccg tactcacctc cgggcacgac gaaggcccgg cctccccgtc
     2041 cgaggacagg aacaccgggc cttcgcagtc tgacggcctg ctgacggcct gatcatgctg
     2101 cccagacccg ttaccgcagg tcaggcaggg ctaccgatca gacgtcgtag tagagggcga
     2161 actcgtacgg g
//
LOCUS       DATACA010000481         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2234853, whole genome shotgun sequence.
ACCESSION   DATACA010000481 DATACA010000000
VERSION     DATACA010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234853"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..139
                     /locus_tag="VHK28_09625"
     CDS             2..139
                     /locus_tag="VHK28_09625"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX2142510.1"
                     /translation="MEQSERFHWLTTPRSTVLQTGPIHTGVTDDPAATFDHLFSRLVE
                     R"
     gene            389..844
                     /locus_tag="VHK28_09630"
     CDS             389..844
                     /locus_tag="VHK28_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX2142511.1"
                     /translation="MHPREGDRPLLERAKEEARRGLAEGGIPIGAVLAADGQVLGAGH
                     NRRVQRGSPILHGETDALEAAGRQPASAYRRATIVTTLSPCDMCTGAILLYGIPRVVI
                     GENRAFMGGEDLLRSRGVEVVVLDDPECVELMEEFIAKHPELWNEDIGE"
     gene            844..1764
                     /locus_tag="VHK28_09635"
     CDS             844..1764
                     /locus_tag="VHK28_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX2142512.1"
                     /translation="MRAFRFAAYTWGAESGAAWTALARRVEELGYDTLLVPDHLGRQL
                     SPVPALAAAAALTTRLRIGPYVFANDFRHPLVMAREAATLDVLSGGRLELGLGAGWRT
                     TDYRQLGYLYPPPGRRIDRLVEALRLVKRLLAGERVTHQGKHYRLGGAVIRPRTVQSP
                     VPLHIGAGGPRMLRLAAREADIVGLIPQFTRRGIPNVRDATEGALGRKVALLREAAGD
                     RFEQLELSIYCADAGVVGRSASPARALASMVKGGLVAPVGSPYLLYGTIGGLRERLLR
                     RRDRLGVTHYAVPAYAMEELAPLVEELAGR"
     gene            complement(1792..2703)
                     /locus_tag="VHK28_09640"
     CDS             complement(1792..2703)
                     /locus_tag="VHK28_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2142513.1"
                     /translation="MAHSSDFDPYRVLGVPRAATLHEIGRAHRRLAKETHPDLHGPDA
                     AARMRAINRAWHLLSDPKRREAWDRAHASGGVAGHWPAPQPGDAAREPSWQPGPTATW
                     SEPWTSPAPARGPTAERSAPATPSVRDSGWLAAGVGLALVVAAILLASVAATSRPPTS
                     LDQAMAEAGLRMGNTVALDSTNALVIREPAPGEVEAAHFYQRPDGSWQEGQRTRVAPS
                     GDNTVSVMAWDGVGRSPAWRTFVFGRSAPGVDRVVLPDRALGTETFDGTWGIGLPDGA
                     LTVSALRWEFLAADGSVVIAGSGQLGG"
     gene            2780..3664
                     /locus_tag="VHK28_09645"
     CDS             2780..3664
                     /locus_tag="VHK28_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2142514.1"
                     /translation="MKVGVVMPIGRESDAHIPGWDELRAVAEAAENGGLDSIWVFDHL
                     LFRFPGQETEGIHEAWTVLAALAAITTRVELGILVAAMPFRNPALLAKMAATAEEVSG
                     GRLILGVGTGWHEPEFDAFGYPFDHRVGRFEEALQILVPLVREGRVRFEGRYHRADAE
                     IIPRYRRPDGRPTPILIAGRRPRMMRLVARYADAWNAAWLGPVAQLEPRVAPLREAMI
                     EEGRDPSTIDITAGVNVITDPAAPVPDHALSGEPQELARELRAYADAGVAHVICALEP
                     ATPAGVERVAQAARLLRE"
     gene            complement(3671..>4049)
                     /locus_tag="VHK28_09650"
     CDS             complement(3671..>4049)
                     /locus_tag="VHK28_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142515.1"
                     /translation="ARRRRAAHHGRAFYAAAFRLYRRHGIPEAVALAGESARYPSSLR
                     HAVALGIPGALPLLVERRTALRARPRRRWRVAVPEHVVRLERDGRWSVCVTCRQRVVG
                     RNLVRIRRARRPVRHVLMSASPA"
BASE COUNT          566 a         1440 c         1481 g          562 t
ORIGIN      
        1 gatggagcaa tcggagcggt tccactggct gaccaccccg cgctcaaccg tgctccagac
       61 cggccccatc cacaccggcg tgaccgatga tccggccgcc accttcgacc acctcttctc
      121 acggctcgtc gagcgctgag ccgtgcggat agcggtggtg actcgcggga tggcgttgtc
      181 aggcggcata tcgggactcg tgcgcggctg actgccggga agcgaatccc gaagcgttac
      241 gccagccagt cgtggttccg cgcacgccgg acgccgctgg tcgtgctcaa ctccgcctct
      301 atccaagcct tgcacggcgc aacgagtgcc gtaacgctcc cccgagtcgc atctcgcaag
      361 tcgggcggct ggcgggtagg gttcaaccat gcatccccgt gagggcgacc gccccctgct
      421 ggaacgcgcc aaggaggagg cgcggcgcgg actggcggag ggcggcatcc ccatcggggc
      481 cgtgctggcc gccgacggcc aggtgctggg cgccggtcac aaccggcgcg tgcagcgggg
      541 cagccccatc ctgcatggcg agacggatgc gctggaggcg gccggtcggc agccggcatc
      601 ggcctaccga cgggcgacca tagtcaccac cctgtcgccg tgcgacatgt gcacgggtgc
      661 gatcctgctg tacggcatcc cgagggtggt catcggcgag aacagggcgt tcatgggcgg
      721 cgaggacctg ctgcgctcgc gcggcgtgga ggttgtcgtg ctggatgacc ccgagtgtgt
      781 cgagctgatg gaggagttca tcgcgaagca tcccgagctg tggaacgagg acatcgggga
      841 gtagtgcgcg cattccgatt cgccgcgtac acctgggggg cggagtccgg ggcggcctgg
      901 acggccctcg cgcggcgtgt ggaggagctc ggctacgaca ccctgctggt gcccgaccac
      961 ctcgggcgcc agctgtcgcc ggtgccggcg ctggcggcgg cggcggcgct caccacgcgc
     1021 ctgcgcatcg gtccatacgt cttcgccaac gacttccggc acccgctggt catggcccgc
     1081 gaggcggcca ccctggacgt gctctccggc ggccgtctgg agctgggtct cggggctggc
     1141 tggcgcacga ccgattaccg acagctgggc tacctctacc cgccgccggg acggcgtatc
     1201 gaccggcttg tggaggcgct gcggctggta aagcgactgc tggccggcga gcgggtcacc
     1261 caccagggaa agcactaccg cctgggtgga gccgtgatcc ggccgcgaac ggtgcagtcg
     1321 cctgtgccgc tgcacatcgg ggccggtggg ccgcggatgc tgcgcctggc ggcccgcgag
     1381 gcggacatcg tggggctgat cccgcagttc acccggcgcg gcatcccgaa cgttcgcgac
     1441 gcgaccgagg gcgcgctggg ccggaaggtg gccctgctgc gggaggccgc cggtgaccgt
     1501 ttcgagcagc tggagctctc catctactgc gccgatgccg gcgtggtcgg ccgcagcgcc
     1561 tcccctgccc gcgccctggc cagcatggtc aagggcggat tggtggcacc ggtcggctcc
     1621 ccgtacctgc tgtacggcac aatcggcggg ctgcgcgagc gcctgctgcg ccggcgggat
     1681 cggctgggcg tcacgcacta cgccgtcccc gcgtacgcaa tggaggagct ggcgccgctg
     1741 gtggaggagc tcgccggacg atgagctgat tgggtcccgt ggagccgctc gtcaaccgcc
     1801 gagctggccg ctgccggcga tcaccacgct gccgtcggct gccaggaact cccagcgcag
     1861 agccgacacg gtcagcgcgc cgtccggcag cccgatgccc catgtcccgt cgaacgtctc
     1921 ggtgcccagc gcgcgatccg gcagcaccac gcggtccacg cccggggcgc tgcggccgaa
     1981 gacgaacgtc cgccaggccg ggctgcgccc caccccgtcc caggccatga cgctgacggt
     2041 gttgtcgccg ctgggcgcga cccgggtccg ctgcccctcc tgccacgatc cgtccgggcg
     2101 ctgatagaag tgagccgcct cgacctcgcc gggcgccggc tcccggataa ccagcgcatt
     2161 ggtgctgtcg agcgccaccg tgttccccat gcgcaggccc gcctcggcca ttgcctggtc
     2221 cagggaggtg ggaggtcgcg aggtggcggc caccgacgcg agcaggatgg cggcgacgac
     2281 cagcgcaagc cccaccccgg ccgccagcca accggagtcc cggaccgatg gagtggccgg
     2341 tgcggaccgc tccgccgtgg gacccctggc gggggccggc gaggtccatg gctcagacca
     2401 ggtggcggtc ggaccaggct gccagctcgg ttcccgggca gcgtcacccg gctgaggagc
     2461 gggccagtgt ccggccacgc cgccgctcgc gtgcgcccgg tcccacgcct cgcgccgctt
     2521 gggatcggag agcaggtgcc aggcacgatt gatggcgcgc atgcgcgccg cggcatcggg
     2581 tccgtgcagg tcgggatggg tctctttggc gagccgtcga tgcgcccggc cgatctcgtg
     2641 cagtgttgcc gcgcgaggca cgccgagcac tcggtaggga tcgaaatccg acgagtgggc
     2701 cacggcccgg cgagcgtatc acggtgaacc ccgatcggcc gccggcggta gccagtgcgg
     2761 ggcgcgaatg gaggtggcga tgaaggtcgg cgtggtgatg cccatcggtc gcgagtcgga
     2821 cgcccacatc cctggctggg atgagcttcg agcggtcgcc gaggcggccg agaacggcgg
     2881 cctggactcc atctgggtct tcgaccacct cctcttccgc ttccccggcc aggagaccga
     2941 gggcatccac gaggcctgga ccgtcctggc cgcgctggca gcgatcacca cccgggtcga
     3001 actggggatc cttgtggcgg ccatgccgtt ccgcaacccg gcattgctgg ccaagatggc
     3061 cgccacggcc gaggaggtga gcggcggacg gctgattctt ggcgtcggca ccggctggca
     3121 cgagccggag ttcgacgcct tcggctaccc gttcgaccac cgggtcggcc ggttcgagga
     3181 ggcgctgcag atcctggtac ccctggtccg cgagggccgc gtcaggttcg agggccggta
     3241 ccaccgggcg gacgccgaga tcatcccgcg ctaccggcgc ccggacggac ggcccacgcc
     3301 gatcctgatc gccggaaggc ggccgcgcat gatgcgcctg gtcgcccgct acgccgatgc
     3361 ctggaacgcc gcctggctcg gccccgtcgc gcagctcgag ccgcgggtcg cgcccctgcg
     3421 cgaggcgatg atcgaggagg ggcgcgatcc gtccaccatc gacatcaccg cgggcgtcaa
     3481 tgtgatcacc gatccggccg cacctgttcc cgaccacgcc ttgagcggcg agccgcagga
     3541 gcttgcccgt gagctgcggg cgtacgccga cgccggcgtg gcccacgtca tctgtgccct
     3601 cgagccagca actcccgcgg gcgtcgagcg ggtcgctcag gccgcgcggc tgttacggga
     3661 atgatttcga tcaggcaggg ctcgcgctca tcagcacatg gcgcacgggc cgcctggctc
     3721 gcctgattcg caccaggttc cggcccacca cacgttgccg gcatgtgacg cacaccgacc
     3781 accgtccgtc gcgctccagc cggacgacgt gctcggggac cgcgacccgc caacgacggc
     3841 ggggacgggc gcgcagcgcg gtacggcgct cgaccagcaa cggcagggcc ccggggatgc
     3901 ccagcgcaac cgcgtggcgc agcgagctcg ggtaacgggc cgactcaccg gccagcgcca
     3961 cggcctccgg aattccatgg cggcggtaga gccggaaagc ggcggcgtag aaggcgcgac
     4021 catgatgtgc cgcgcgacgg cgccgcgcc
//
LOCUS       DATACA010000482         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1511777, whole genome shotgun sequence.
ACCESSION   DATACA010000482 DATACA010000000
VERSION     DATACA010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511777"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VHK28_09655"
     CDS             <1..393
                     /locus_tag="VHK28_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEX2142516.1"
                     /translation="SSPKLLALNTALISATARTSMAKARADHEFWSRLVETAVGAHLW
                     AHADPDELTYWREGNAEVDWVVRPGKLLRGRDEPVAVEVRTGHPRGSRGTVAFTQAHR
                     GARSLLVGEGGIPLEDFLLRHPDEWLHG"
     gene            407..790
                     /locus_tag="VHK28_09660"
     CDS             407..790
                     /locus_tag="VHK28_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX2142517.1"
                     /translation="MSGETVQAWLDRYVEAWRTNDPEQVRALFTDDAVYRYRPYGGER
                     HAVRGLNPIVGNWLEEQDPPGSWEARYEPYAVDGDRAVAAGYSRYYASDKGPERTFHN
                     VFLLRFDADGRCAEFTEYYMREEPE"
     gene            795..1649
                     /locus_tag="VHK28_09665"
     CDS             795..1649
                     /locus_tag="VHK28_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2142518.1"
                     /translation="MGVGDRGSVGVSGAAGRVWSEVGDRVFVRRYAFFDQSIGAVIGQ
                     AGVVLIDTRTTYAQADELRNDLALLTDAPLAAVVNTHHHYDHTFGNARFGDAPIWGHD
                     ACAIALSQHGEQMRRRVMAKLPEIADELAEVVITPPGRTFARAQQLDLGNRQIELRHL
                     GRGHTDNDIVVLVPDADVLFAGDLLENRAPPSFGDAFPIAWAGTVGERLLPLVGEVVV
                     PGHGDPAGRDFAVRQAAELALLARLIRESTAGAIGLDEVLRASPFPAEQTRVAVERGH
                     LELEQPPQ"
     gene            complement(1658..2164)
                     /locus_tag="VHK28_09670"
     CDS             complement(1658..2164)
                     /locus_tag="VHK28_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX2142519.1"
                     /translation="MNHRSGTDETRIVELTPQPTVAVRLRQPMSDLDLGKLFDLHLPN
                     VAHRIADLGGEAVGAPYGRYHEWGPTDVDVEIGIPVRAPVANLRPVEEAEPGEAAASE
                     LPGGRAAVTVHRGTYDGLPQTYDRLRSWIGQQGQQPGGAPWESYVDDPSEVEDPGQLR
                     TEVVMPLA"
     gene            2245..>2915
                     /gene="guaA"
                     /locus_tag="VHK28_09675"
     CDS             2245..>2915
                     /gene="guaA"
                     /locus_tag="VHK28_09675"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2142520.1"
                     /translation="MSEEQRPAAAQRGRTVHGEPANAGHRVLAPDDAEVEAYLEQALH
                     APPPTRADEAAERPDTVVILDFGSQFAQLIARRVRELNVYSELLPHDTPWVEIERRQP
                     KAVILSGGPASVYEEGAPKADPAIWSGRVPVLGICYGLQLMALELGGEVVPSTKREYG
                     PASVQITSEDGLFAGLEREQPVWMSHGDTILRPPEGFRATAQTDSTAFAGLIDPVRGL
                     YGIQF"
BASE COUNT          412 a         1027 c         1062 g          414 t
ORIGIN      
        1 tcgtcgccca agctgctggc gctcaacacc gcgctcatct ccgccacggc gcgcaccagc
       61 atggccaagg cccgggccga ccacgagttc tggagccgcc tggtcgagac cgcggtcggc
      121 gcgcacctgt gggcccacgc cgatccagac gagctcacct actggcggga gggaaacgcg
      181 gaggtggact gggtggtgcg acccggcaag ctgctgcgcg gccgggacga gccggtcgcg
      241 gtcgaggtgc gtaccgggca ccctcgcggg tcgcgcggca cggtggcctt cacccaggcc
      301 catcgcggcg cccggtcgct gctcgtcggc gagggcggta tcccgctcga ggacttcctg
      361 ttgcggcacc ccgacgagtg gctgcacggc tgataggatg cgagctgtga gtggagagac
      421 cgtccaggcc tggctggacc gatacgtcga ggcctggcgc accaacgacc cggagcaggt
      481 tcgcgcgctc ttcaccgacg atgccgtgta ccgctaccgc ccgtacggcg gcgaacggca
      541 cgccgtgcgg gggctcaatc cgatcgtggg caactggctc gaggaacagg atccgcccgg
      601 gagctgggag gcgcgctacg agccgtacgc ggtcgatggc gatcgcgcgg tggcggccgg
      661 gtacagccgc tactacgcgt ccgacaaggg gccggagcgc accttccaca acgtcttcct
      721 gctgcgtttc gacgccgacg gccgctgtgc cgagttcacc gagtactaca tgcgcgagga
      781 gcccgaatga gccggtgggt gtcggggacc gcggttcggt tggggtgagc ggcgccgccg
      841 gccgcgtctg gtccgaggtc ggcgatcggg tcttcgtccg ccgctacgcg ttcttcgacc
      901 agagcatcgg cgcggtcatc ggtcaggcgg gtgtggtgct catcgacacc cgcaccacct
      961 acgcccaggc ggacgagctg cggaacgacc tggccctcct gaccgatgcg ccccttgccg
     1021 cggtggtcaa cacgcaccac cactacgacc acaccttcgg caacgcccgc ttcggggacg
     1081 cgcccatctg gggccacgac gcctgcgcca tcgccctgtc gcagcacggc gagcagatgc
     1141 ggcggcgggt catggccaag ctgccggaga tcgccgacga gctggccgag gtggtcatca
     1201 cgccgcccgg gcgcacgttc gcccgggcgc agcagctgga cctgggcaac cgccagatcg
     1261 agctgcgcca cctgggtcgg ggtcataccg acaacgacat cgtggtgctg gtgcccgatg
     1321 cggatgtgct cttcgccggt gacctgctcg agaaccgcgc gccgccgtcc ttcggcgatg
     1381 cctttccgat cgcctgggcc gggacggtcg gggagcggct gctgcccctg gtcggcgagg
     1441 tggtggtgcc gggccacggt gatccggcgg gccgagactt cgcggtacgc caggcggcag
     1501 agctggccct gctggcacgc ctcatccggg agtcgacggc cggcgccatc ggcctggacg
     1561 aggtgctgag agcctcaccg tttccggctg agcagacccg ggtcgcggtg gagcgcggcc
     1621 acctcgagct cgagcaacct ccgcagtagc ggcgtgctca ggccaggggc atgaccactt
     1681 cggtgcgcag ctggccgggg tcctccacct ccgaggggtc gtccacgtac gactcccagg
     1741 gcgcgccgcc cggctgctgg ccctgctgcc caatccagct ccgcagccgg tcgtaggtct
     1801 gcggcagccc gtcgtaggtg ccgcggtgga cggtgacggc cgcgcgcccg ccgggaagct
     1861 ccgacgcggc ggcctcgcca ggctccgcct cctcgacggg gcgcaggttg gcgaccggcg
     1921 cgcgtaccgg gatgccgatc tcgacgtcga cgtccgtcgg cccccactcg tggtagcgcc
     1981 cgtacggggc cccgaccgcc tcgcccccga ggtcggcgat gcggtgggct acgttgggca
     2041 ggtgcaggtc gaagagcttg cccaggtcga gatcggacat cggctggcgc aggcgcacgg
     2101 ccacggtggg ctgcggcgtc agctcgacga tgcgcgtctc atcggtcccg gatcggtggt
     2161 tcacgacgtg cgccccacgc tgcaggttcg gtgcccgaaa gcctagactc tccggcacca
     2221 ccatcatcgc cgagcggagg ccgagtgagc gaggagcagc gcccagcagc ggcgcagcgg
     2281 ggacgcacgg tgcatggcga gccggccaat gccggtcacc gcgtcctggc cccggacgac
     2341 gcagaggtcg aggcctacct ggagcaggcg ctccacgccc ctcctcccac ccgcgccgac
     2401 gaggcggcgg agcgcccgga caccgtcgtc atcctggact tcggcagcca gtttgcccag
     2461 ctcatcgcca ggcgggtgag ggagctgaac gtctactcgg agctgctgcc gcacgatacg
     2521 ccgtgggtcg agatcgagcg gcggcagccg aaggcggtca tcctgtccgg cggaccggca
     2581 tcggtctacg aggagggcgc gccgaaggct gacccggcca tctggtctgg ccgcgtgccg
     2641 gtgctcggca tctgctacgg gctgcagctg atggcgctcg agctgggggg cgaggtggtg
     2701 cccagcacca agcgggagta cgggccggca tcggtccaga tcacctccga ggacggcctg
     2761 ttcgccgggc tggagcgcga gcagccggtg tggatgagcc acggcgatac catcctccgg
     2821 ccgccggagg gatttcgggc cacagcccag accgactcga ctgccttcgc cggcctgatc
     2881 gatcccgtcc gcggcctgta cggcatccag ttcca
//
LOCUS       DATACA010000483         6391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1993494, whole genome shotgun sequence.
ACCESSION   DATACA010000483 DATACA010000000
VERSION     DATACA010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1993494"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VHK28_09680"
     CDS             complement(<1..779)
                     /locus_tag="VHK28_09680"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2142521.1"
                     /translation="MTNLPVLSLVVFTPLVGVLVLLFIPGANHRTIRWVSLAAAVASL
                     GFSLFLLGYDPAGAEFQFREDLEWIPAFGMGYRLGVDGLSVVLVLLTTVLSIVAMVYS
                     WEPIQTRVKEFYATMLLLMIGMLGVFVSLDLFLFYVFWEISLIPMYLVIGIWGGPRRI
                     YATVKFVIYTLVGSLLMLVAILAVAIAHAAGGNEFTFAYETLRGFPFAEGLQALAFIA
                     FFLAFAIKVPMWPLHTWLPDAHVEAPTAGSIILAGVLLKLGG"
     gene            complement(776..2986)
                     /gene="nuoL"
                     /locus_tag="VHK28_09685"
     CDS             complement(776..2986)
                     /gene="nuoL"
                     /locus_tag="VHK28_09685"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2142522.1"
                     /translation="MELLIALIPALPIAGFLFAVLVGPRLDRIPLHGHGHDDHGTDAA
                     HDSHAPADDSGFRGIPTEEEQAATSPHAGHHDDLANASGADGVIPEDLNDGLGQSGHV
                     TPGGEQPVYRSWIVPVTLVAISWVLSMIVFADVVFGGNEHHVTLYEWIGSGDFRVEIA
                     FFVDALTAMLLLVVSTVGLLVHVYSIGYMNGDRGFWRFFAYLNLFMFSMLLLILGDNF
                     LMLYVGWEAVGLCSYLLIGFWYKKPSAAGAAKKAFVVNRIGDFGFGLGIMMIWTLLGT
                     LQFDEVFAAIGGLEEWQITVIALLLFAGAVGKSAQFPLHVWLPDAMEGPTPVSALIHA
                     ATMVNAGVYMVARANPIFAESPIAMWVVASIGVFTAIFAAAIALTQNDIKKVLAYSTV
                     SQLAYMFFALGIGAWVAAIFHLVSHGFFKGLLFMGSGSVIHGASGEQDIRFLGGLRDR
                     MKWTYRTMLIGSLALAGIPIFAGFFSKDEILAEAFNRGYYLFYAVGVIVAFMTAFYTF
                     RMMYMTFHGRWRGTREAWRHVHESPPTMVAPLIILAVPTVLVGLLLGIPPEGGLIHTW
                     LGEVFHHAEEVLHAGVLPGSILAGAIEHGEHHGFELFGIGGLLLLVGAAAGVLGIALA
                     YRWYVRDTAAPERFVRRIPFGLGPGMYAASVNKYYVDDIYQLVFARGGVLLSQLLWWF
                     DARVIDGAVNGAGWIAQRIGGAGRRVQTGRVQNYGLGIASGLLVVLVAYLVTRG"
     gene            complement(2999..3304)
                     /gene="nuoK"
                     /locus_tag="VHK28_09690"
     CDS             complement(2999..3304)
                     /gene="nuoK"
                     /locus_tag="VHK28_09690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2142523.1"
                     /translation="MGLTGYLIVSTLLFAIGAFGFLARRNAILMLICIELMLNAVNLS
                     LVAFNAFSPAGEVAGAIFALMIMAVAAAEATVGLALVIAIIRTRQTAEVDHYADLRE"
     gene            complement(3304..3747)
                     /locus_tag="VHK28_09695"
     CDS             complement(3304..3747)
                     /locus_tag="VHK28_09695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2142524.1"
                     /translation="MTLRNIIHSAVAMMVCFGSLAGMYLLLGAGIVAAAQVLIYLGAI
                     SVLILFAIMLTQAGDANLPAPFHRQVWFAALLTVATAGLIIWSVVSTDWGAQAEAVSV
                     VLESLATLLFTDYALPLEIISLLLLAAIIGAIFLARRPEEDERAN"
     gene            3945..4355
                     /locus_tag="VHK28_09700"
     CDS             3945..4355
                     /locus_tag="VHK28_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX2142525.1"
                     /translation="MTSTGSRAAVVALGAYLALVLAVTLVPVPGGPHELNLVPTEGIR
                     RTYQDKGSAFGTVQVVGNLLMLAPAGALLRFAFPRFRLRDAALAAAALSIGIELVQWQ
                     VAIGRAADIDDVWMNTLGAAVGHAAGAGVASRLR"
     gene            4395..5561
                     /locus_tag="VHK28_09705"
     CDS             4395..5561
                     /locus_tag="VHK28_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142526.1"
                     /translation="MTGPATRLWTPAFIALALAELAYFTAAGLTIPLTPLFAEGPLGA
                     TEVWVGITVGAFSVTAVVLRPYAGRLADRRGRRPLLIGGALLVVVTLAAHAVTTELAL
                     LIVLRLVLGVAEAFFFVAGFAALADLAPPGRAGEAISFNSLALYLGLAFGPLIGELLL
                     DAGGFGVAWLGGAGLALLAVLLALTLPETAPHDRAEADPGPLIHRAALGPGLALFAGV
                     TAMAGFLGFVALHARDLRLDGSSGALLLFGLIVVGCRIVFARLPDRVPPYRLGTASLA
                     VIAAGLATIGLVNSPAGLLAGAAVTAVGVAFITPAMFAAVLARVPASERGSALGTVSA
                     FIDLAFGGGPILVGVVAGASSIPVGFMAAAGVAAIGAVGAALALRPREAPATAT"
     gene            5641..6261
                     /locus_tag="VHK28_09710"
     CDS             5641..6261
                     /locus_tag="VHK28_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142527.1"
                     /translation="MNADAVSALRGLAWAVISFAFFLVALFATLPILAAVRLFIATDH
                     LVEMAEWSVVWGALSLCGVLVAGRIAFGDWLRVGLPGFLVGGTGIALSAIVHVVLQQW
                     AITRFGYFDADFVGWWTGGLFAVLIGLATGAFGVFVAPRGAAAWPLIAVLLGFAGVLL
                     VLLGNLSGLADGIAPESWPLAIWLGISALYAAGAATSATVRVRAAR"
BASE COUNT          986 a         2176 c         2239 g          990 t
ORIGIN      
        1 ccgcccagct tgagcagcac gccagccagg atgatggaac cggcggtggg cgcctccacg
       61 tgcgcgtcgg gcagccaggt gtgcagcggc cacatcggga ccttgatggc gaaagccaag
      121 aagaaggcga tgaaggccag cgcctgcagc ccctcggcga aggggaagcc gcgcagcgtc
      181 tcgtaggcga aggtgaactc gttgccgccc gctgcgtgcg cgatggccac cgccaggatg
      241 gcgaccagca tcagcagcga gccgaccagc gtgtagatca cgaacttgac cgtggcgtaa
      301 atgcgccgcg ggccgcccca gatcccgatg accaggtaca tcgggatgag gctgatctcc
      361 cagaagacgt agaacaggaa caggtccagg ctgacgaaca cgcccagcat gccgatcatg
      421 agcagcagca tggtcgcgta gaactccttg acccgcgtct ggatcggctc ccaggagtac
      481 accatggcca cgatcgacag caccgtggtc agcagcacca agaccaccga caggccatcc
      541 acccccaggc ggtagcccat gccgaaggcc gggatccact ccaggtcctc gcggaactgg
      601 aactcggcgc ctgccgggtc gtagccgagc aggaagaggc tgaatccaag gctggccacg
      661 gccgccgcca ggctcaccca gcggatggtc cgatggttgg cgccggggat gaacagcagg
      721 acgagcacgc cgaccagcgg cgtgaacacc accaggctca ggacgggcag gttggtcatc
      781 cgcgcgtcac caggtaggcg accagcacca ccagcagccc gctggcgatg cccagcccgt
      841 agttctggac ccgaccggtc tgcacccgac gcccggcccc gccgatgcgc tgcgcgatcc
      901 agccggcgcc gttgaccgcc ccgtcgatca ccctggcgtc gaaccaccac agcagctgcg
      961 acagcaggac gccgccgcgc gcgaacacga gctggtagat gtcatccacg tagtacttgt
     1021 tcacgctggc ggcgtacatg ccggggccga ggccgaaggg gatgcggcgc acgaagcgct
     1081 ccggggctgc cgtatcgcgc acgtaccagc ggtacgccag ggcaatgccc agcacgccgg
     1141 ccgcggcacc caccagcagc agcaggccgc cgatcccgaa cagctcgaag ccgtggtgct
     1201 ctccgtgctc gatggccccg gccaggatcg acccgggcag gacgccggcg tgcagcacct
     1261 cctccgcgtg gtggaagacc tcgcccagcc aggtgtggat caggccgccc tccggcggga
     1321 tgcccagcag caggccaacc agcacggtgg gcaccgccag gatgatgagc ggcgcgacca
     1381 tggtgggcgg cgactcgtgc acgtggcgcc acgcctcgcg cgtcccgcgc caccgtccgt
     1441 ggaaggtcat gtacatcatg cggaaggtgt agaaggcggt catgaaggcc acgatcaccc
     1501 cgaccgcgta gaagaggtag tagccgcggt tgaatgcctc ggccaggatc tcgtccttgc
     1561 tgaagaaccc ggcgaagatc gggatgccgg ccagcgccag cgagccgatc agcatggtgc
     1621 ggtaggtcca cttcatccgg tcgcgcaggc cgcccaggaa gcggatgtcc tgctcgccgg
     1681 aggcgccgtg gatcaccgag ccggagccca tgaacagcag ccccttgaag aagccgtggc
     1741 tgaccaggtg gaagatggct gccacccagg ccccgatgcc cagcgcgaag aacatgtagg
     1801 ccagctggct caccgtcgag taggcgagca ccttcttgat gtcgttctgg gtcagcgcaa
     1861 tggcggcggc gaagatggcg gtgaagacgc cgatgctggc caccacccac atggcgatcg
     1921 ggctctcggc gaagatcggg ttggcgcggg ccaccatgta gaccccggcg ttgaccatgg
     1981 tcgccgcgtg aatcagggcg ctgaccgggg tggggccctc catggcgtcc ggcagccaga
     2041 cgtggagcgg gaactgggcc gacttgccca ccgcgccggc gaacaggagc agggcgatga
     2101 ccgtgatctg ccattcctcc agcccgccga tggccgcaaa cacctcgtcg aactggagcg
     2161 tgccgagcag ggtccagatc atcatgatcc ccagcccgaa gccgaagtcg ccgatgcggt
     2221 tgacgacgaa ggccttcttt gcggcgccgg cggccgacgg cttcttgtac cagaagccga
     2281 tgagcaggta gctgcacagg ccgaccgcct cccagcccac gtacagcatc aggaagttgt
     2341 cgcccaggat cagcagcagc atgctgaaca tgaacaggtt caggtaggcg aagaagcgcc
     2401 agaacccgcg gtcgccgttc atgtagccga tggagtacac gtggaccagc aggcccacgg
     2461 tgctgaccac cagcagcagc atggcggtca gggcgtccac gaagaacgcg atctccaccc
     2521 ggaagtcccc ggatccgatc cactcgtaga gcgtgacgtg gtgctcgttg ccgccgaaga
     2581 cgacgtccgc gaagacgatc atgctcagga cccaggagat ggccaccagc gtcaccggca
     2641 cgatccagga gcggtagacc ggctgctcac cgcccggggt gacgtggccg gactgcccca
     2701 gcccgtcgtt caggtcctcg gggatgaccc catcggcgcc ggaggcgttg gccaggtcgt
     2761 cgtggtggcc agcgtgcggc gaggttgccg cctgctcctc ctcggtgggg atgccgcgga
     2821 agccggagtc gtcggccggc gcgtggctgt cgtgggccgc atcggtcccg tggtcgtcat
     2881 gtccgtggcc gtggaggggg atgcggtcca gccgcggccc gaccagcacg gcgaacagga
     2941 agccggcgat gggcagcgcg gggatgaggg cgatcagcag ctccatgagt cccgtccgct
     3001 attcccgcag gtccgcgtag tggtcgactt cggcggtctg gcgagtgcgg atgatggcga
     3061 tcaccagcgc cagcccgacg gtcgcctcgg cggcggccac ggccatgatc atgagcgcga
     3121 agatggcgcc ggccacctcg ccggcgggcg agaaggcgtt gaaggcgacc aggctgaggt
     3181 tgaccgcgtt cagcatcagc tcgatgcaga tcagcatcag gatcgcgttg cgccgcgcca
     3241 ggaagccgaa ggcaccaatg gcgaacagca gcgtggagac gatcaggtag ccggtcagtc
     3301 ccatcagttc gccctctcgt cctcctccgg gcggcgcgcc aggaagatgg cgccgatgat
     3361 ggccgccagc agcagcaggc tgatgatctc gagtggcagt gcgtagtcgg tgaagagcag
     3421 ggtggccagg ctctccagca cgaccgatac cgcctccgcc tgggctcccc agtcggtgct
     3481 gaccaccgac cagatgatca gcccggcggt ggccaccgtc agcagagcgg cgaaccagac
     3541 ctggcggtgg aacggggccg gcaggttggc gtcgccggcc tgggtgagca tgatggcgaa
     3601 caggatcagc acgctgatgg cgcccaggta gatcagcacc tgcgcggcgg ccacgatgcc
     3661 agcgcccagc agcaggtaca tgccggcgag cgagccgaag cagaccatca tggcgacggc
     3721 ggagtgaatg atgttccgca acgtcacgac ggccaggcca ccgccgatga cgccgatggc
     3781 gagcaggacg aagaggactc ctgagacgtc caggtcgagg ctccccttgg ggttctcgcg
     3841 atttcgagcg ctgaaacggc ctcaagttat tcgctcgagg ccgtgaccga ttctagcgca
     3901 ggcgccgctg accgggggtt cggcggatca gccatcatct ggccatgacc tctaccggct
     3961 cccgtgctgc cgtggtcgcg ctgggcgcgt acctcgccct ggtgcttgcc gtcaccctgg
     4021 ttccggtccc gggcgggccg cacgagctca acctggtccc gaccgagggc atccgccgca
     4081 cctaccagga caaggggtct gccttcggca ccgtacaggt tgtcggcaac ctcctgatgc
     4141 tcgccccggc cggggcgctg ctccggttcg ccttcccgcg gttccgcctg cgcgacgcgg
     4201 cgctcgcggc ggcggcgctg tcgatcggga tcgagctggt gcagtggcag gttgccatcg
     4261 ggcgggccgc cgacatcgac gacgtgtgga tgaacaccct gggcgcggcg gtcggccatg
     4321 cggccggcgc gggggtagca agccggctgc gctgaccggc cgtccacctt gaccgcgcgt
     4381 gcaacatggt tccgatgacg ggtccagcaa cccggctctg gacgccggcg ttcatcgcgc
     4441 tggcgctcgc cgagctggcg tacttcacgg ccgccggcct caccatcccc ctcacgcccc
     4501 tgttcgcaga gggaccgctg ggtgccaccg aggtgtgggt gggcatcacc gtcggcgcgt
     4561 tcagcgtgac cgcggtcgtc ctgcggccgt acgccggacg tctcgccgac cgccgcggcc
     4621 gacggcccct gctcatcggt ggtgctttgc tggtggttgt caccctggca gcccacgcgg
     4681 tgacgaccga gctggcactg ctcattgttc tgcgcctggt gctgggcgtg gcggaggcgt
     4741 tcttcttcgt ggccggcttt gccgccctgg ccgacctcgc gccacccggc cgcgccggag
     4801 aggcgatcag cttcaactca ctggcgctct acctgggcct cgccttcggg ccgctgatcg
     4861 gcgagctgct gctggacgcc ggcggattcg gcgtggcctg gctgggtggg gcgggactgg
     4921 cgctgctggc cgtgttgctg gcgctgaccc tgcccgagac cgcgccgcac gatcgtgccg
     4981 aggctgatcc ggggccgctc atccatcgtg ccgccctcgg ccctggtctg gcgctgttcg
     5041 ccggcgtgac cgccatggcc ggcttcctgg gcttcgtggc gctgcacgcg cgcgacctgc
     5101 ggctggacgg ctcgagcggc gccctgctgc tcttcgggct gatcgtggtc ggctgccgga
     5161 tcgtcttcgc ccgcctgccc gaccgggtgc cgccctaccg cctggggacg gcttcgctgg
     5221 cggtgattgc cgccggcctg gccaccatcg gcctggtgaa cagccccgcc ggcttgctgg
     5281 cgggggcggc tgtcacggcg gttggcgtcg ccttcatcac gcccgccatg ttcgccgccg
     5341 tcctggcgcg ggtgcccgcc tcggagcgtg ggtcggcgct cggcacggtg agcgcgttca
     5401 tcgacctcgc cttcggcggt ggaccgatcc tggtcggcgt ggtggcgggc gcctccagca
     5461 tccctgttgg cttcatggct gcggccgggg tggcggccat cggcgccgtc ggcgcggcac
     5521 tcgcccttcg acctcgagag gctccggcga cggccacctg aggggctttc cgccctggcc
     5581 ggcgggccgg gccgattcgc ccgccggcaa cgctcgcccg gccgtgttcg tcttgatgcc
     5641 gtgaacgcgg acgctgtctc ggcacttcgc ggcctggcct gggccgtcat ttcgttcgcg
     5701 ttcttcctcg tggccctgtt cgccacgctg ccgatcctcg ccgccgtccg cctgttcatt
     5761 gccaccgacc acctggtcga gatggcggag tggtcagtgg tatggggggc gctgtcactc
     5821 tgcggcgtgc ttgtggcagg gcgcatcgcc ttcggagact ggctacgagt cggtttgcct
     5881 gggttcctcg tgggcggaac cgggattgcg ctgtcggcga tcgttcacgt cgtcctgcag
     5941 cagtgggcga tcacgcgctt cgggtacttc gacgccgact tcgttggatg gtggactggc
     6001 ggcctgttcg cggtgctcat cggcctggcg accggcgctt tcggcgtctt cgtcgcacca
     6061 cggggcgccg ccgcctggcc cttgatcgcc gtcctgctcg gctttgccgg cgtcctactc
     6121 gtgctccttg gaaatctctc cggtctggcg gacgggatcg ctccggagag ctggccgctg
     6181 gcaatctggc tggggatcag tgctctgtac gcagctggcg cggccacctc cgcgactgtc
     6241 cgcgtgcgag ccgcccggta gggcaagcca agagcatcaa cggcgagacc ggcctatccg
     6301 ctctgtcgcc tgaaccgcaa gctatttcgt ggatgaggcg tgggcgcgcg gccgcaggct
     6361 gcggcaattg ccagccaagg agtcgtgagc c
//
LOCUS       DATACA010000484         7442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_228110, whole genome shotgun sequence.
ACCESSION   DATACA010000484 DATACA010000000
VERSION     DATACA010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228110"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VHK28_09715"
     CDS             <1..257
                     /locus_tag="VHK28_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX2142528.1"
                     /translation="ALRVQLERDAGGGVPSLREYARRWGLDELRMRRVAPGAPLLHPG
                     PTNEGVEVSADLAAGRRSLIGRQVANGVAVRMAVLALLAQ"
     gene            254..1642
                     /locus_tag="VHK28_09720"
     CDS             254..1642
                     /locus_tag="VHK28_09720"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX2142529.1"
                     /translation="MTRFGTLSDPPSALLITSARVLDPVAESDAVRDLAIAGGRFVEP
                     ADLPGEAARVDGSGLLAIPALCDLHAHLRDPGGEAAETLESGSQAAAHGGYASVCAMP
                     NTEPALDDAARVRDVLARAAATSCRVRVIAAATRGRGGEQPAELGELAEAGAVGFSDD
                     GAAVASSRTARAIMAYMAPLGLPLIEHAEDAELAAGSAMRAGAIATRLGLGGWPASAE
                     LAIVERDIALAEETGAWVHFTHLSTAASVEAVRRAKARGLRVTCDVTPHHLAMTDAWV
                     AGHRAFAWEEPGGLAAEPHGGALDEQLAYDGSCRVNPPLPTRDDALALLAGVVDGTVD
                     AIATDHAPHPPERKAVEFAVAAPGMIGLETALSVGLTAVAAGCLGLAELVAALSTRPA
                     ALIGETRGLQPGAVADLALVDPMARWRVDPTTLASRSRNTPLLGRELPGVVRLTLAGG
                     RLTYVDNLPPLS"
     gene            1729..2040
                     /locus_tag="VHK28_09725"
     CDS             1729..2040
                     /locus_tag="VHK28_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142530.1"
                     /translation="MSIRYNEELDPNEGPGFGCFIWQAILFAVILLLIPVGLFTLGWP
                     VWVLVVLLVIDLILLFFVSMTAIFLLRVFFADRRRGRGRTTGVARPPRMVSTKDEETD
                     D"
     gene            2080..2397
                     /locus_tag="VHK28_09730"
     CDS             2080..2397
                     /locus_tag="VHK28_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142531.1"
                     /translation="MVTFFNVRKGRDAIFKRGMRHIFPRTARRYEAEYGIRFIGWFNV
                     TEGSEWDNMIILDLPNYGVLDRLYADPSTRGFGHRVAESIFDRKQTMFLRERMGPDYV
                     FKP"
     gene            2394..3215
                     /locus_tag="VHK28_09735"
     CDS             2394..3215
                     /locus_tag="VHK28_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142532.1"
                     /translation="MSAPRITALRELAGEAELERRDFVYESTEQLRRFIDSNRERLRE
                     IGPIKLVDDDQDYLLYHPVDGTWTTRLTYQDPADNEWYDEEQIVDAFSEVVELYNPAD
                     IFSWFMEAARDPMNHLLPADELQSEEQLVEHEGGDDWQSGLPATQQETLAAQRLWQLA
                     DAYRAQIGQQQKQSLREFAANAEEVLSRTGDLVLLDETDDVLVLRSDGRFETGLDLTD
                     LPAGSALDVESRRAGLTVADIRVIADFYDPMDILQWVTEALSERYPAVDFSAVYE"
     gene            3226..3825
                     /gene="pyrE"
                     /locus_tag="VHK28_09740"
     CDS             3226..3825
                     /gene="pyrE"
                     /locus_tag="VHK28_09740"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX2142533.1"
                     /translation="MLAEPRSATIEEIFDAAGALQRGHFQLKSGRHSDRYLEKFAVLQ
                     YPALAAEIGRRLATDLAPMEPTLVVGPTTGGVLLAYETARQMSAMAGRQVRGVFAEPI
                     ARGARALRRGWPVADDERVVLVDDILTTGASLAETASAVRAVGVTPLAATVIVDRSTG
                     PVEVGCPVRSLGRIEIASWEAADCPLCARGEPVVKPGSS"
     gene            complement(4023..4880)
                     /locus_tag="VHK28_09745"
     CDS             complement(4023..4880)
                     /locus_tag="VHK28_09745"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX2142534.1"
                     /translation="MTEPAARPARSVPVLIGGSEMAEIDEAVQRLGLSQDALMEAAGA
                     AVTEVALAELSRVSEPVRGPGGALTDPPLTVILCGPGNNGGDGFVAARRLASAGRRVA
                     AVFVGDATRIGHSGTAAAHNWTVLQAMASAGSLDLYVAPTPELLLQLRARLSPASLVV
                     DALLGTGASGPLREPISTAVDLLNAIRTHASAAGRPCSVLAVDTPTAIDQTDGSRSSP
                     VVAADLTVTFHRAKAGFALDPEARRLAGRYFVAPIGIPLEAEEGIVPPDGEFPPARIA
                     QVNWQEPGE"
     gene            complement(4877..5332)
                     /locus_tag="VHK28_09750"
     CDS             complement(4877..5332)
                     /locus_tag="VHK28_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX2142535.1"
                     /translation="MTVHTPIETRSRGLPAWAIGITVLVLLVGGVYMAGNLTGENPPI
                     AAQPSASGGPNNEQLARQIIADGQCAACHGQDLTGGVGPDLHGIANGPKSENLGDLAA
                     EFPDDWIAMWIDGTDPAVADLDRAGMPRFGETLTPDQIAVVAEYLLTLQ"
     gene            complement(5349..6848)
                     /locus_tag="VHK28_09755"
     CDS             complement(5349..6848)
                     /locus_tag="VHK28_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX2142536.1"
                     /translation="MAARPPHVRNPKKLRPYDEPPRPRRPLIPDAPDSAATAFLVLSL
                     LWLAAATGIGVLWGAMLLAPEQLSTTFEVPLPIIGALEVELSPQTVKSGFTNAIVFGW
                     LSNAAFAAICFIVPRITGARLGGDAIAFLSAGAWNVAVAAGLASVYLPTLTPDGPLAE
                     FPLPVDGLLLLGAFGITIVFLRTLVTARQRLPYVSVWFFGLALLALMGAYTLASVPPL
                     LGLDQTASLLITGFAARAIQTYWVLGVTLGTLFYIVPRATGNPLASGGMAWLTWLLWA
                     AFSGLSALGALVDPSVPYAVTSIGNVGTILLVAPVFLAVTNLAMTMHGRWTVALTAGT
                     AAFAAVSMAFLLGAALLEAIGALRSVQGLVRNTEWVTGLWLFTTLGSATFALLALVDH
                     AAPRVLRRDWRGSFLTDAQLWLTFTGTTLAALALMGGGIANGSLVAEGAPLEEITATL
                     QWFWYGAFAGLGLAALGGLAALVNLFVMYTTARRAEYAPVGTPAAAAGH"
     gene            6980..7054
                     /locus_tag="VHK28_09760"
     tRNA            6980..7054
                     /locus_tag="VHK28_09760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7012..7014,aa:Met,seq:cat)
     gene            7156..>7442
                     /locus_tag="VHK28_09765"
     CDS             7156..>7442
                     /locus_tag="VHK28_09765"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX2142537.1"
                     /translation="MILVCGATGLLGSRIVDRLRERDVPVRALVRPRTDATALEAVGA
                     EIARGDVRDTASLRAAVQGVSSVVSTVNTVARVMAGEKGLSIREVDDRGHA"
BASE COUNT         1100 a         2610 c         2687 g         1045 t
ORIGIN      
        1 tggccctccg cgtccagctg gagcgcgacg ctggcggcgg cgttccgagc ctcagggagt
       61 acgcccgccg ctggggtctg gacgagttgc gcatgcgccg cgtcgcgccc ggcgcgccgc
      121 tcctgcatcc cggacccacc aacgagggcg tcgaggtcag cgccgacctg gccgccgggc
      181 gccgcagcct gatcggtcgc caggtggcca atggggtcgc cgtgcgaatg gccgtcctgg
      241 ctctgctggc gcagtgaccc gcttcggcac tctgtccgac ccgccgtccg cactcctgat
      301 cacaagtgca cgcgtcctcg accccgtcgc cgagtcggac gccgtccgcg acctggcgat
      361 cgccggcggc cgcttcgtgg agcctgccga tctgcccggg gaggctgctc gcgtggacgg
      421 gtccggcctg ctggccatcc ccgcgctgtg cgatttgcat gcccacctgc gcgaccccgg
      481 cggggaggcg gcagagacgc tggagagcgg gtcgcaggcg gcggcgcacg gcggctacgc
      541 cagcgtgtgt gccatgccga acacggagcc ggccctggac gatgccgcgc gcgtccggga
      601 tgtgctggcc cgggccgctg ccacctcctg ccgcgtacgg gtcatcgcgg cagccaccag
      661 ggggcgcggc ggagagcagc cagcggagct cggcgagctg gccgaggcag gggccgtggg
      721 attcagcgac gacggggccg ccgtggcgtc gtcgcgcact gcacgcgcca tcatggccta
      781 catggcgccg ctcggcctgc cgctcatcga gcacgccgag gatgccgagc tggctgccgg
      841 gagcgccatg cgcgccggcg ccatcgccac ccggttgggg ctcggcggtt ggccggcgag
      901 cgccgagctg gcgatcgtgg agcgggacat cgcgctggcc gaggagaccg gcgcctgggt
      961 ccacttcacc cacctgtcca ccgcggcgtc ggtggaggcc gtgcggcggg ccaaggcccg
     1021 cgggctgcgc gtgacctgcg acgtcacgcc ccaccacctg gccatgaccg atgcctgggt
     1081 ggccggccat cgcgccttcg cctgggagga acccggcggc ctggcggccg agccgcacgg
     1141 cggagcgctc gacgagcagc tggcgtacga cggctcgtgc cgagtcaacc cgccgctccc
     1201 tacccgcgac gacgcgctgg cgctcctcgc cggggtcgtg gacgggacgg tggacgccat
     1261 cgccaccgac cacgcgccac acccacccga gcgcaaggcg gtggagttcg ccgtggccgc
     1321 gccgggcatg atcgggctgg agaccgcgct gtcggtcggg ctgacggcgg tggcggccgg
     1381 ctgcctggga ttggccgagc tggtcgccgc cctcagcacg cgtccggcgg cgctcatcgg
     1441 tgagactcgc gggctccagc cgggagcggt cgccgacctg gcccttgtgg accccatggc
     1501 ccgatggcga gtggacccca ccaccctcgc atcgcgctca cggaacacgc cgctgctcgg
     1561 gcgggagctg cctggcgtcg tgcggctcac gctggcgggt ggccggctga cctacgtgga
     1621 caaccttccg cccctcagct gaggcacgga tggcacgtcg tcatgcggtg cttcctcgga
     1681 tatgggacca tcccgacgcc tgtgaacgcc acccgtcctg agtgatgcat gtcgattcgc
     1741 tacaacgagg agctggaccc gaacgagggt ccgggcttcg gatgcttcat ctggcaggcg
     1801 atcctgttcg ccgtcatcct gctgctgatc ccggtcggcc tgttcaccct gggttggccg
     1861 gtgtgggtgc tggtcgtcct gctggtcatc gacctgatcc tgctgttctt cgtgagcatg
     1921 accgcgatct tcctgctgcg cgtcttcttc gccgatcgcc ggagagggcg ggggcgtacc
     1981 accggcgtcg cgcgcccgcc ccggatggtg agcaccaagg acgaggagac cgatgactga
     2041 tccccatcct gcccgtggga agccctgacc atgccgctga tggtcacctt cttcaacgtg
     2101 cgcaagggcc gcgacgccat cttcaagcgc ggcatgcgcc acatcttccc gcgcaccgcc
     2161 cgccgctacg aggccgagta cgggattcgc ttcatcggct ggttcaacgt caccgagggc
     2221 tcggagtggg acaacatgat catcctggac ctgccgaact acggcgtgct ggaccgcctc
     2281 tacgccgacc cctcgacgcg tggcttcggg caccgggtgg ccgagtccat cttcgaccgc
     2341 aagcagacca tgttcctgcg cgagcggatg gggcccgact acgtgttcaa gccatgagtg
     2401 cgccccggat caccgccctg cgcgagctgg cgggcgaggc cgagctggag cgccgcgact
     2461 tcgtctacga gtccaccgag cagctgcgca ggttcatcga cagcaaccgg gagcggctgc
     2521 gggagatcgg tcccatcaag ctggtggacg acgatcagga ctacctcctc taccacccgg
     2581 tcgatggcac ctggaccacc cggctgacct accaggaccc tgccgacaac gagtggtacg
     2641 acgaggagca gatcgtcgac gccttcagcg aggtcgtgga gctctataac ccggccgaca
     2701 tcttctcgtg gttcatggag gccgcccgcg acccgatgaa ccaccttctg cccgccgacg
     2761 agctgcagtc cgaggaacag ctcgtggagc acgagggagg cgatgactgg cagtcggggc
     2821 tgccggccac ccagcaggag acgctggccg cccagcggct gtggcagctg gccgacgcgt
     2881 atcgggccca gatcggacag cagcagaagc agtcgctgcg cgagttcgcc gccaacgcag
     2941 aggaggtcct gtcccgcacc ggtgacctcg tcctcctgga cgagaccgat gacgtcctgg
     3001 tcctgcgctc ggacggccgg ttcgagaccg gcctggacct caccgacctg cccgcaggct
     3061 ccgccctgga cgtggagtcg cgacgcgccg gcctcacggt ggcggacatc cgggtgatcg
     3121 ccgacttcta cgatcccatg gacatcctgc agtgggtgac cgaggcgctc tccgagcgtt
     3181 acccggcggt cgacttcagc gcggtatacg aatagcttcg gaccgatgct cgccgagccg
     3241 cgaagcgcga ccatcgagga gatcttcgac gccgccggcg ccctgcagcg cggccacttc
     3301 cagctcaaga gcggccgcca ctcggaccgc tacctggaga agttcgccgt cctgcagtac
     3361 ccggcgctgg ccgcggagat cggtcgccgg ctggccaccg acctggcacc gatggagccg
     3421 accctggtgg tcggccccac caccggcggg gtgctgctcg cctacgagac ggcgcgccag
     3481 atgtcggcga tggccggtcg ccaggtgcgc ggggtgtttg ccgaaccgat tgcccgcggc
     3541 gcgcgggccc tgcgccgtgg ctggccggtg gccgacgacg agcgggtggt gctggtggac
     3601 gacatcctga ccaccggcgc ctcgctggcc gagacggctt ccgccgtccg tgccgtgggg
     3661 gtcactccgc ttgccgccac cgtcatcgtc gaccgctcca ccgggccggt ggaggtgggc
     3721 tgcccggtcc ggtcgttggg ccgaatcgag atcgcctcgt gggaggctgc ggattgcccg
     3781 ctctgcgcgc ggggcgagcc ggtggtcaag cccggtagca gctagatgac agccggtccc
     3841 cggcggaacc ggctgtgcgg ctgtgcggcg caaaggggcc gacgcgggtg cgagcgcacc
     3901 cggtcgccca gcaccgtcag gggtcgattg tgcggctgtg cggctgtgcg gctgtgcggc
     3961 acaaagaggc ctgatgcggc cgaggcgcgc acccagcggg tccggcgccg agccgaaacc
     4021 cgctactccc cgggctcctg ccaattgacc tgggcgatcc gtgcgggcgg gaactcgccg
     4081 tccggcggca cgatcccttc ctccgcctcg agcgggatgc cgatcggcgc cacgaagtag
     4141 cggccggcca ggcgccgcgc ctcgggatcc agcgcgaagc cggccttggc ccgatggaag
     4201 gtgacggtga ggtcggcagc gaccaccggg gaggatcggg aaccatcggt ctggtcgatg
     4261 gcggtcgggg tgtccacggc gagcaccgag cagggccgcc cggcggccga cgcgtgggtg
     4321 cgaatggcgt tcagcaggtc gacggcggtc gagatcggtt cacgcagggg accggatgcg
     4381 ccggtgccca gcagtgcgtc caccaccagc gacgccgggg agaggcgggc gcggagctgg
     4441 agcagaagct cgggggtggg ggccacgtag aggtcgagcg aaccggccga cgccatggcc
     4501 tgcagcacgg tccagttatg ggccgcggcc gtgccgctgt ggccgatccg ggttgcgtcg
     4561 cccacgaaca ccgctgccac ccgtcggcca gccgaggcca ggcgacgtgc ggccacgaag
     4621 ccgtcgccgc cgttgttccc cggcccgcac aggatgacgg tcagcggcgg atcggtgagg
     4681 gcgccgccgg gcccgcggac cggctcgctg acccgcgaca gctcggccag ggcgacctcg
     4741 gtgaccgccg cgccggccgc ctccatcagc gcgtcctggg agaggccgag gcgctggacg
     4801 gcctcgtcga tctccgccat ctccgagccc ccgatgagga cggggacgct gcgcgccggc
     4861 cgagcggccg gttcggtcac tgcagggtca gcagatactc ggcgacgacg gcgatctggt
     4921 cgggtgtcag cgtctcgccg aagcgcggca tcccggcccg gtccaggtcg gcaacggctg
     4981 gatcagtgcc gtcgatccac atggcgatcc agtcgtcggg gaactcggcg gccagatccc
     5041 ccaggttctc gctcttcggt ccgttcgcga tgccgtgcag gtcgggtccc acgccgccgg
     5101 tcaggtcctg cccatggcac gccgcgcact ggccgtcggc gatgatctgg cgcgccagtt
     5161 gctcgttgtt gggcccgccg ctggccgagg gctgggcggc gatcggtggg ttctcgccgg
     5221 tgaggttgcc agccatgtag acgccgccga ccagcagcac caggacggtg atgccgatcg
     5281 cccaggcggg caggccacgg gagcgggtct cgatgggggt gtggacggtc atgcctcgcg
     5341 ctcgctcctc aatggccagc cgcggcggcc ggcgtcccga ccggcgcgta ctccgcgcgg
     5401 cgggccgttg tgtacatcac gaacaggttg accagcgccg ccaggccacc cagcgcggcc
     5461 aggcccagcc ctgcgaaggc gccgtaccag aaccattgga gcgtggcggt gatctcctcg
     5521 agcggagcgc cctccgccac cagcgagccg ttggcgatcc cgccgcccat cagggccagc
     5581 gcggccaggg tggtgccggt gaaggtcagc cacagctggg catcggtcag gaagctgccg
     5641 cgccagtccc ggcgcagcac gcgcggagct gcgtggtcca ccagcgccaa cagggcgaag
     5701 gtggcggagc ccagcgtcgt gaagagccac agcccggtga cccactcggt gttgcgtacc
     5761 aggccctgca ccgagcgcag ggcgccgatc gcctccagca gggcggcacc cagcaggaag
     5821 gccatcgaca ccgcggcgaa cgcggcggtg cccgccgtga gcgcaaccgt ccatcggcca
     5881 tgcatggtca tcgcaaggtt cgtgaccgcc aggaagactg gcgccaccag caggatggtg
     5941 cccacgttgc cgatgctggt caccgcgtac ggcaccgatg gatcgaccaa tgcgcccagc
     6001 gccgagagtc cggagaaggc cgcccacagc agccaggtga gccacgccat gcccccactg
     6061 gccagcggat tgccggtggc ccgcggcacg atgtagaaca gcgtccccag ggtcacgccg
     6121 agcacccagt aggtctggat ggcccgcgcc gcgaacccgg tgatcagcag gctggcggtc
     6181 tggtccagcc cgagcagggg tgggaccgat gccagcgtgt acgcgcccat cagcgccagc
     6241 agcgcaagcc cgaagaacca gacactgacg tacggcagcc gctgtcgagc cgtcaccagc
     6301 gtccgcagga agacgatggt gatgccgaac gcccccagca gcagcaggcc atccaccggc
     6361 agcgggaact cggcgagtgg tccgtccggg gtcagggtcg gcaggtagac cgatgccagt
     6421 cccgccgcga ccgcgacgtt ccaggcgccg gccgacagga aggcgatggc gtcgccgccc
     6481 agccgcgcac cagtgatacg cggcacgatg aagcagatcg ccgcgaaggc ggcgttcgag
     6541 agccagccga agacgatggc gttggtgaag cctgatttaa ccgtctgcgg gctgagctcg
     6601 acctccagcg caccgatgat cggcaacgga acctcgaacg tggtcgacag ctgctcggga
     6661 gcgaggagca ttgcgcccca cagcaccccg atgccggtgg ccgcggccag ccacagcagc
     6721 gacagcacaa ggaaggcggt cgcggcgctg tcgggggcat ccggaatcag cggacggcgc
     6781 ggcctcggcg gctcgtcgta cggacggagc ttcttcgggt ttctgacgtg tggtggtctg
     6841 gcagccatcg gcgcgcgcaa gaacctcctg cggacgtacg gggcccggtc gagagcgacc
     6901 tcgggcggca ttgtagccga ggtgctgcga cagtggcctc cgtttgaccg atcgatcgtg
     6961 gaccggtacc attcgtggcc gcggggtgga gcagtggtag ctcgtcgggc tcataacccg
     7021 aaggtcgtcg gttcgaatcc gtcccccgca accaaagact cccagatccg aaggccgcct
     7081 cccgccaggc ggccttcgtg ctgcgtgtac catgccgggg ctgcccgccc acgaacgtcc
     7141 agtgaggaaa cgcccatgat cctcgtgtgc ggtgccaccg ggctgcttgg ctcgcggatc
     7201 gttgaccggc tgcgcgagcg agacgtgccg gtacgtgccc ttgtccggcc gagaaccgat
     7261 gcgaccgccc tcgaggccgt aggagccgag atcgcccgcg gtgacgtgcg tgataccgcc
     7321 agcctgcggg ccgccgttca gggagtctcg agcgtcgtgt ccacggtcaa caccgtcgcc
     7381 cgcgtgatgg caggtgagaa ggggctcagc attcgtgagg tggatgatcg aggccacgcc
     7441 ag
//
LOCUS       DATACA010000485         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2796810, whole genome shotgun sequence.
ACCESSION   DATACA010000485 DATACA010000000
VERSION     DATACA010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2796810"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="VHK28_09770"
     CDS             complement(<1..1153)
                     /locus_tag="VHK28_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX2142538.1"
                     /translation="MRTERFTQRTNEAIAAAQQLAEGEGHAELTATHLLLSLVDQPDG
                     VVPAVLEKIGVAPAAVAAAAREQLGRLPKVHGAGTQLSLGNDARRILSDAHPLAANMH
                     DEYVSTEHLLLAMLQDSAGNDARRLLEAQGVQAASVLEALTAIRGSQRVTSENPEATY
                     QALEKYGRDLTDAARRGQLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQRIVRGDVPEGLRDKRVVALDLGALVAGAKYRGEFEERLKAVLKEITDSAGQIV
                     LFIDELHTVVGAGAAEGSMDASNLLKPMLARGELHAIGATTLDEYRKHIEKDAALERR
                     FQPIVVDQPGVEDTISILRGLRERYEVHHGVRITDSALVAAAMLSNRYIT"
     gene            complement(1153..>2194)
                     /locus_tag="VHK28_09775"
     CDS             complement(1153..>2194)
                     /locus_tag="VHK28_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2142539.1"
                     /translation="PMEYKDYYQILGVPKTATQAEIKKAYRKLARELHPDRNPGDAAA
                     EKRFKDANEAHAVLADPEKRKHYDELGANWQAYQQSGFGDGSTDWGGFGGAPGGTRWE
                     YRRVSPEDLGGFSDFFRTFFGGEAFGAGPIGAGGAGAGGSTAGFEQIFDFGGRGGTTM
                     RRTAAMPEVQARAEVTLAEVARGTERMVDVDGRRIQVTIPAGVNDGARIRLRGAGLRQ
                     GGQGEGASGAGDLVVTVNVRPDPRFERRGADLVTELPLTLSEAMLGAEVPVATPTGSV
                     KLRIRPNTQNGQEIRVAGRGLPKKGGAGKGDLVVRTRVQLPELDDEGRARLADVLARH
                     PQPDPRRNGKVH"
BASE COUNT          261 a          797 c          780 g          356 t
ORIGIN      
        1 cggtgatgta gcggttggag agcatggcgg ccgccaccag cgccgagtcg gtgatgcgca
       61 ccccgtggtg cacctcgtag cgctcgcgca ggccgcgcag gatggagatg gtgtcctcca
      121 ctcccggctg gtccaccacg atcggctgga agcggcgctc cagcgcggcg tccttctcga
      181 tgtgcttgcg gtactcgtcc agcgtggtgg caccgatggc gtgcagctcc ccgcgggcca
      241 gcatcggctt gagcaggttg gaggcgtcca tggagccctc ggcggcgccg gcgccaacga
      301 ccgtgtgcag ctcgtcgatg aacagcacga tctggccggc cgaatcggtg atctccttga
      361 ggaccgcctt caggcgctcc tcgaactccc cgcggtactt ggcgccggcc accagggcgc
      421 ccaggtccag ggcgacaacc cgcttgtcgc ggagcccctc gggcacgtcg ccacgcacga
      481 tgcgctgcgc cagcccctcg gcgatggccg tcttgcccac gccgggctcg ccgatcagca
      541 ccgggttgtt cttggtacga cggctgagca cctggatgac gcgacggatc tcctcgtcgc
      601 ggccgatcac cggatcgagc tgcccgcggc gggccgcatc ggtcaggtct cgtccgtact
      661 tctccagggc ctggtaggtt gcctccgggt tctcgctggt cacgcgctgg ctcccgcgaa
      721 tggctgtcag ggcttcaagg accgatgctg cctgcacgcc ctgcgcctcc agcaggcgcc
      781 gggcgtcgtt gccggcggag tcctgcagca tggcgagcag caggtgctcg gtcgagacgt
      841 actcgtcgtg catgttcgcc gccagcgggt gcgcgtcgga caggatgcgg cgcgcgtcgt
      901 tgcccaggct gagctgggtg cccgcgccgt ggaccttggg gaggcggccg agctgctcgc
      961 gggcggcggc cgccaccgcg gccggcgcca cgccgatctt ctccagcacg gcgggcacca
     1021 cgccgtccgg ctggtccacc aggctgagca gcaggtgagt ggcggtcagc tcggcgtggc
     1081 cctcgccctc cgccagctgc tgggcggcgg cgatggcctc attggttcgc tgcgtgaatc
     1141 gttccgtgcg catcagtgca ccttgccgtt tcgtcgcggg tccggctgcg gatggcgtgc
     1201 cagcacgtcc gccagccggg cacggccctc atcgtccagc tccggcagct gcacacgggt
     1261 gcggaccacc aagtcgccct tgccggcgcc gcccttcttc ggcaggccgc gacctgcgac
     1321 ccggatctcc tgcccgttct gggtgttggg ccgaatgcgg agcttgacgc tccccgtggg
     1381 ggtggccacc gggacctcgg cgccgagcat ggcctccgac agggtgagcg ggagttcggt
     1441 caccaggtcg gcgccacggc gctcgaagcg cgggtcgggg cggacgttga cggtgaccac
     1501 caggtcgccg gcgccgctcg cgccctcgcc ctggccgccc tggcgcaggc ccgcgccgcg
     1561 caggcggatg cgggccccgt cgttgacgcc ggccgggatc gtgacctgga tgcggcgccc
     1621 gtccacgtcc accatgcgct cggtgccgcg ggccacctcg gccagggtca cctctgcgcg
     1681 ggcctgcacc tccggcatgg ccgccgtcct gcgcatggtt gtacccccgc ggcctccgaa
     1741 gtcgaagatc tgctcgaagc cggccgtcga gccgccggcg ccggcgccgc cggcgccaat
     1801 cggtccagcg ccgaaagcct ccccaccgaa gaaggtgcgg aagaagtcgc tgaagccgcc
     1861 caggtcctcc gggctgacgc gccggtactc ccagcgcgtg ccgcccgggg caccgccgaa
     1921 gccgccccag tccgtcgagc cgtcgccgaa gccggactgc tggtaggcct gccagttggc
     1981 gcccagctcg tcgtagtgct tgcgcttctc cggatcggcc aggacggcgt gcgcctcgtt
     2041 ggcgtccttg aaccgcttct cggcggcggc gtcccccggg ttgcggtcag ggtggagctc
     2101 acgcgccagc ttgcgatagg ccttcttgat ctcggcctgg gtcgcggtct tgggcacgcc
     2161 aaggatctgg tagtagtcct tgtactccat cggt
//
LOCUS       DATACA010000486         4528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1272103, whole genome shotgun sequence.
ACCESSION   DATACA010000486 DATACA010000000
VERSION     DATACA010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272103"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1208)
                     /locus_tag="VHK28_09780"
     CDS             complement(138..1208)
                     /locus_tag="VHK28_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142540.1"
                     /translation="MPVTLQFDLWQWGLIFAASAAAVMAAGTVLARAGDAIATKTRLG
                     GLFVGMLLMASATSLPEIITDVSAAVAGAPDLAIADLFGSSMANMAILAVVDLTARRQ
                     LLGSVELGHARVGAIAIALTALVVLGVVSPTGIRIGWIGIEPVLVVVAYVLAAAWMGR
                     SQSREATGSTTTSRPLPAEAPLPVADELINPVGWGVDPSRPVRRDLLSFAGAAMVIVV
                     AGPALALSAQGIAEETGIAQTFIGVTLVAIATSLPELVASLAAVRLGAHDLAVGNLFG
                     SNAFNMAIIMWVDIAYVDGPVLQAVSPQQLVPGLGAILLMGLAVALIVSGERSRLRRG
                     EPSTVLLLLAYVGLVALSWIRA"
     gene            complement(1317..2918)
                     /locus_tag="VHK28_09785"
     CDS             complement(1317..2918)
                     /locus_tag="VHK28_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX2142541.1"
                     /translation="MTAAAYEETVHPDGRHELTPAAQAALDDSPIYNEDLAPVRIEQR
                     TWSTYAYMALWIGMSVNIPSWTLAAGLIALGMDWVQAVFTVMLGNVIVLVPMLLISHA
                     GTKYGIPYPVFARAAFGVIGANLPALIRAGVACGWFGIQTWIGGGAIFFLLAAIFGEG
                     SWWTTAGTLAIGFGDPQPWTLWLSFIIFWAINLAIVLKGMNTLRIFESWSAPFLIVIS
                     VALTVYMVMQAGGLGPILAQESGFGGWTGEFWAIFFPSLMAMIAFWSTLSLNMPDFTR
                     FGRSQRDQTIGQALGLPTTMTVFAILAVFTASAATIVYAASSGVDVWNPVALVAEIGN
                     PVVIIVALFAIALATLTTNVAANLVSPSYDFSNTFPKLISFRTGGIITAIISVLIQPW
                     YLISNPEVYIFTWLGFYGGGTGAIAGVLIADYWWVRRTKLRLADLFRTDGGAYKYAAG
                     WNWRALVALAVGIILAVGGAYTAPGTQGPFPADGLIPFLKPLYDYSWVVGLVAGFLVY
                     AGLHMISPVTERGPAEEVTPSPEMA"
     gene            complement(3387..>4528)
                     /locus_tag="VHK28_09790"
     CDS             complement(3387..>4528)
                     /locus_tag="VHK28_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2142542.1"
                     /translation="SQLMCVNIGHADDRVVKAIQEQAAVLAYANPFMATEPRARLAAK
                     LAEITPGDIEVFFFTNGGAEANENAIKIARFVTGRHKIMARYRSYHGGTAGAITLTGD
                     PRRWAAEPGIPGVVRVRDPYHGIQAGWDTADEALAELEEVIQLEGPNTIAGFILETVT
                     GTNGILVPPDGYLQGVREICDRYGIMMIADEVMAGFGRTGKWFAVDHWNVVPDLITMA
                     KGLTSAYVQLGAVGMRRKYAEHFNERVFYGGLTYNSHPLACATALATIAVYEEEGLLQ
                     RAVEMGGIMRATWTSSRRDTRRSGRCAASVCSGSWSWCATGRPWSRWRPSTAPRMRWP
                     RWAAASERTVSTPWCAGTTSSRTRRWSSARRRWRRASASSIGHWR"
BASE COUNT          790 a         1561 c         1486 g          691 t
ORIGIN      
        1 gccatcctca ccacgctgat cgtgccgccg ctccttccgg ttctcctacg ccgggccgag
       61 ccgcaaggga tgccggagaa ggagccagtc gtcgtcccgg ccggcacgga ggacgatgac
      121 ggagttcgag agggtaatta ggccctgatc caggacagcg ccacgagccc aacgtaggcc
      181 aacagcagga ggacggtgct cggctcgccc cgccggagcc ggcttcgctc gccgctgacg
      241 atcagcgcta ccgccagccc catcagcagg atggcgccca agcccgggac cagctgttgt
      301 ggggagaccg cctgcagcac cggaccatcc acgtaggcga tgtccaccca catgatgatc
      361 gccatgttga acgcgttgct gccgaaaaga tttccgacag ccaggtcatg ggcgccgagg
      421 cgaacggccg ccagggatgc caccagctcc gggagggacg tggcgatggc caccagcgtg
      481 acgccgatga aggtctgggc gatgcccgtc tcctcggcaa tgccctgcgc cgaaagcgca
      541 agcgccggtc cagccacgac gatcaccatt gccgcgccgg cgaagctgag caggtcacgt
      601 ctgaccggcc ggctggggtc tactccccag cccaccgggt tgatgagctc atcggctact
      661 ggcaggggcg cctcagccgg gaggggtcgc gaagtggtgg tgctgcccgt ggcctcgcgg
      721 ctctgcgacc gccccatcca tgcagcggca agcacgtagg ccacgaccac cagtacgggc
      781 tcgatcccga tccatccgat ccgaatcccg gtcgggctca cgactcccag tacaaccagg
      841 gcggtgagcg caatggcgat ggccccgact cgtgcgtgcc caagctccac gcttccaagg
      901 agctgccgcc gggcggtgag gtcgacgact gccaggattg ccatgttggc catgctgctg
      961 ccgaacaggt cagcgatcgc gagatccgga gcgccagcga ccgctgcgct tacgtcggtg
     1021 atgatctcgg ggagcgaggt ggccgacgcc atgagcagca tccccacgaa gaggccgccc
     1081 aacctcgtct tcgtggctat cgcgtcccct gcccgtgcca gcaccgttcc ggctgccatc
     1141 accgccgccg ccgaggcggc gaagatcagg ccccattgcc agagatcgaa ctgaagggtc
     1201 acgggcatcc tcgggtgcat tgcctgctgg gccgcagaga tatggcgcgc cagccatacg
     1261 cgggaccggg gcccctggcg ggaccccggt cggggttcgc gtcagcgaat gagaacttac
     1321 gccatctccg gtgagggagt gacttcctcc gcaggaccac gctccgtgac cggcgaaatc
     1381 atgtggaggc cggcgtagac caggaagcca gccaccaggc caaccaccca gctgtagtcg
     1441 tagagtggct tgaggaacgg aatcaacccg tccgcgggga acggcccctg cgtcccgggt
     1501 gcggtgtatg cgccacccac ggccaggatg atgccgacgg ccagggcaac cagcgcccgc
     1561 cagttccagc cggcggcgta cttgtaggcc cctccatcgg tcctgaacag gtcggccagc
     1621 cgcagcttgg tgcgccggac ccaccagtag tcggcgatca gcactccggc gatggcgccc
     1681 gtgcccccgc cgtagaagcc gagccaggtg aagatgtaga cctcggggtt gctgatcagg
     1741 taccagggct ggatcagcac gctgatgatg gccgtgatga tcccacccgt cctgaagctg
     1801 atcagcttcg ggaaggtgtt cgagaagtcg tacgacgggc tcaccaggtt ggcggccacg
     1861 ttcgtggtga gtgtggccag cgcgatggcg aacagggcca cgatgatcac gaccgggttg
     1921 ccgatctcgg ccaccagcgc gaccggattc cagacgtcga cgccgctgct cgcggcgtac
     1981 acgatcgtcg ccgccgaagc ggtgaagacc gccaggatgg cgaacaccgt catcgtggtc
     2041 ggcaggccca gcgcctgacc gatggtctgg tctcgctgcg agcgcccgaa ccgggtgaag
     2101 tccggcatat tgaggctcag cgtggaccag aaggcgatca tcgccatcag cgatgggaag
     2161 aagatcgccc agaactcgcc ggtccagcca ccgaagccgc tttcctgcgc caggatcggc
     2221 ccgaggccgc cggcctgcat gaccatgtat accgtcaggg ccacagagat gacgatcagg
     2281 aatggcgctg accagctctc gaagatccga agggtgttca tgcccttcag gacaatggcc
     2341 aggttgatcg cccagaagat gatgaagctc agccacaggg tccagggctg cggatcaccg
     2401 aagccgatcg ccagcgtgcc ggcggtcgtc caccacgacc cctcgccgaa gatggcggcc
     2461 agcaggaaga agatcgcgcc gccgccgatc caggtctgga tgccgaacca tccacacgca
     2521 acgcctgccc tgatgagggc cggcaggttg gcaccgatga cgccgaacgc ggcgcgagcg
     2581 aacaccgggt atgggattcc gtacttggtg ccggcatggc tgatcagcag catcggcacc
     2641 agcacgatga cgttcccgag catcaccgtg aacacagcct gcacccagtc catgccgagc
     2701 gcgatcaggc cggccgccag ggtccagctc gggatgttga ccgacatgcc gatccacagc
     2761 gccatgtagg cgtaggtgct ccacgtgcgc tgctcgatgc gcaccggggc caggtcctcg
     2821 ttgtagatcg gactgtcgtc gagcgccgcc tgggcagccg gcgtcagctc gtgacgacca
     2881 tcgggatgaa ccgtttcctc gtacgcagca gcagtcatgc gcctcctccg gtaccagtcc
     2941 ctccataccg gcgtaccgag tacgccgcca atacgttact cgggcatgca agagcctttc
     3001 tgtacgccgc cgtggcgggg cgcagccgtg cgtaaccgtc cgaattcacg ccaagtgcgc
     3061 agtaggggcc gttgcggccc tgcgggccgt ccggattcac gccacccgag aattcggccc
     3121 cgtcagcggc ccaccaggcg acggaacgcg cacctcacat gaatccgacc cgttcgcgcc
     3181 gggcacgggg ttcgcgctgg gtccaacctg ccaggttcag aacaggtgcg cacaggaccg
     3241 atggagaggc cacgccaccg ccagttgcag gttcgggcgc gcgcgatggc gcccaccgcg
     3301 gcatcccatg atcgtcatgc gcgcctcgcc tgaatccggc gacccccggc gctacccgac
     3361 gcgctacccg acgacggccg cgtcagctac ctccagtgcc cgatcgatga tgccgaagcc
     3421 ctccgccatc tcctcctcgc tgatgaccag cggcgggttc gtgaagaagt agttccagcg
     3481 caccagggtg tagagaccgt cctctcggag gcggcggccc agcgcggcca tctcatccga
     3541 ggtgccgttg aagggcgcca tcggctccat ggtctcccgg ttgcgcacca gctccacgat
     3601 cccgaacaga ccgatgctgc gcaccgcccc gaccgacggg tgtcgcgccg cgagctcgtc
     3661 caggtggcgc gcatgatgcc ccccatctcg accgcacgct ggagcagccc ctcctcttcg
     3721 tacaccgcga tcgtggccag cgccgtcgcg caggccagcg ggtgcgagtt gtaggtcaac
     3781 ccgccgtaga agacgcgctc gttgaagtgc tcggcgtact tgcgccgcat gccgacggcg
     3841 cccagctgca cgtaggcgct ggtcagcccc ttggccatgg tgatcaggtc gggaaccacg
     3901 ttccagtggt cgaccgcgaa ccacttaccc gtgcggccga agccggccat cacctcgtcg
     3961 gcgatcatca tgatcccgta ccggtcacag atctcgcgaa cgccctgcag gtagccatcg
     4021 ggcgggacga ggatgccgtt ggtgccggtc accgtctcca ggatgaagcc ggcgatcgtg
     4081 ttcggcccct ccagctgtat gacctcttcc agctcggcca gcgcctcgtc cgcggtgtcc
     4141 cagccggcct ggatgccgtg gtacgggtcg cgcacgcgga ccacccccgg gatgccgggc
     4201 tcggcggccc agcggcgcgg gtcgccggtc agcgtgatgg cgccggcggt gccgccgtgg
     4261 tacgagcggt atcgggccat gatcttgtgg cgcccggtca cgaagcgggc gatcttgatg
     4321 gcgttctcat tggcttcggc gccgccgttg gtgaagaaga agacctcgat gtcgcccggg
     4381 gtgatctccg ccagcttcgc cgccagcctg gcgcgcggct ccgtggccat gaacgggttc
     4441 gcgtaggcga ggacagcggc ctgctcctga atcgccttca ccacgcggtc gtccgcgtgg
     4501 ccgatgttga cgcacatcag ctggctgt
//
LOCUS       DATACA010000487         5538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1593689, whole genome shotgun sequence.
ACCESSION   DATACA010000487 DATACA010000000
VERSION     DATACA010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593689"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VHK28_09795"
     CDS             complement(<1..927)
                     /locus_tag="VHK28_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX2142543.1"
                     /translation="MSVPTDPRWELDERRNARIRQLREQREAPLRRRRRAQPVVLLAW
                     FAGVIALVGVLLFLGFNAFAPRLYAWVEENPGSIEHGIVRDFVEWYRPQALADTPASD
                     SPERVTVDVQLGSSDGQIGQQLFDMGLVHTQLAFHNAVLQAGREGTLAAGTYDLSPSM
                     RPSEIVAALQGEAGEEVTITLQEGWRLEEVVGYLATTPLTMNLDEFLTLVETPPADLL
                     NQYDFFADLPAGRTLEGYLYPDTYRLDANWSAREVLQRILDNFEGRFTQEIRDGIAAQ
                     GLSVDEAVTVASIVEREAVLDEERPLIAGVYLN"
     gene            complement(924..1373)
                     /gene="ruvX"
                     /locus_tag="VHK28_09800"
     CDS             complement(924..1373)
                     /gene="ruvX"
                     /locus_tag="VHK28_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX2142544.1"
                     /translation="MSVFIGLDHGSRRIGVAVGDSQTGMAFARPAIRRRNLEADLAAV
                     AELAASEGASLVVVGLPRNMDGSEGTQAAAARAFGDCLAQIGLAVDYQDERLTSWEAG
                     ERLAAAGRRPQRRSGELDSAAARLILQEYLDALPRPTQAPDPAQEAE"
     gene            complement(1370..2953)
                     /locus_tag="VHK28_09805"
     CDS             complement(1370..2953)
                     /locus_tag="VHK28_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142545.1"
                     /translation="MAGDTQLLYLEPDDEVTTVVRRLRETDVNRVVLVAPGRSKVAGS
                     AVSLRLLAAVAAEEERELALVADAAGRGLAGEAGIPSFATVAEASADGATPLEAPPPS
                     RARIHVVRGDEATSAVPVAAAPAAAAEETQAVRLAPAAPVAVAPRAAAGGMGATRLPR
                     ALIGAAVALVLLAGAAIAAAAPSATITIVPGTVPIGPRAIEVRPPLQVPETDTLEVSR
                     EGAASGERVERTAAAGTVTFLNWNTVRVEVPEGTELSVAGGTRFVTTQTVEVPNGQFT
                     PQGTVQAGEASAPVLAAEPGPGGNVAAEAIDTVESRNVMVFLRGFPNNPRRLVVNPAA
                     TAGGSEEHIPVVLQEDVDAVAGQVAAELTRLRDEAFAGEPERIYPPMEPPRPAVEIPD
                     GLVGSEGQETFSLTGTLAYARPYVLRDDAEEAARGELLADPAAPAGTTLLSETVSVEL
                     GDARLEGAEVVVEAVVAARAVHDLDTDAIRERVTGLTEPEVRQALGDIGQVEVELWPG
                     WVDRVPRLPFRVSVEVSGG"
     gene            complement(2959..4257)
                     /locus_tag="VHK28_09810"
     CDS             complement(2959..4257)
                     /locus_tag="VHK28_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEX2142546.1"
                     /translation="MQQLFGEVVGRLRGRPVAGRTDNGPPALTALDVGTEFAKALVVG
                     VGPDGNGGMVRGSGRQRQGLTHMQSGTVADIDAVVENCRLALDAAEAQAGLRPSAAVI
                     GIAGELVKGTTSTVTLRRDQAQRPLSEPELELLVQRVQATALDEAEQRIAWESGVERL
                     EVRLVHAAIVEMKIDGYPVSNPVGFTGSQVELAVFNAFAPMVHLGALQAVAHRLGLHL
                     LGVIAEPYAVATCLDPGEVGDAGAVFVDVGGGTTDVALVRRGGIAGTKMLALGGRAFT
                     KGLAERFGLSFARAEALKLAAGTAELPEAVDRHELDAALAEDAAIWRRGVELMISDLA
                     EGELLPARVYLCGGGAELPQVTAALAEDAWWKQLPFARRPQVLALGPRDVTGVLDATG
                     SLGTPQDVTPMALAHQALMLDAQAGVAQRAMRSAVRAMQL"
     gene            complement(4293..>5538)
                     /locus_tag="VHK28_09815"
     CDS             complement(4293..>5538)
                     /locus_tag="VHK28_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX2142547.1"
                     /translation="YPNEVSLDVARVVGVAAADGTTQVVLDRTPFYAEGGGQIGDRGE
                     IIGPRGKLAVEDTQRAGEAIVHVGRVEGELAAGDVVRAMVDEDRRRSAARNHTATHLL
                     HRALRDVLGEQAKQAGSWVGPEGLRFDFPASAATPRQALRYVERRVNEQIRRAASVQP
                     EWMSLDEAQRMGADMFFGEKYVPDRVRVVRVDGYSKELCGGTHVATTGEIGSFLVTGE
                     SSIGAGLRRIEAVTGAAAEELVARRLAALREAAQALGVADEAVPARVEALSHRLREAE
                     KAAREPRTGGARPDARAALQGAQQAGETQVIVQHYPEADANALRGWVDELRGVTGRFV
                     AVASGGGNGAALVVAASRDLAAEGFDSVAVARAAAAILGGGGGGRPELAQAGGRDAGR
                     LEEALREAARLALEALEPIEAA"
BASE COUNT          698 a         2210 c         1879 g          751 t
ORIGIN      
        1 gttcaggtag acgccggcga tgagaggccg ctcctcgtcc aggaccgcct cgcgctccac
       61 gatgctggcc accgtcaccg cctcgtccac cgacagcccc tgcgcagcga ttccgtcgcg
      121 tatctcctgc gtgaagcggc cctcgaagtt gtccaggatg cgctgcagca cctcgcgcgc
      181 cgaccagttg gcgtccaggc ggtaggtgtc cgggtagagg tagccctcca gcgtgcggcc
      241 cgcgggcagg tcggcgaaga agtcgtactg gttcagcagg tcggccggcg gcgtttccac
      301 cagcgtcagg aactcgtcca ggttcatggt cagcggcgtg gtggccaggt agccaaccac
      361 ctcctccagc cgccaccctt cctggagggt gattgtgact tcctcgccag cctccccctg
      421 cagggcggcg acgatctcag atggccgcat ggacggcgac aggtcatagg tgccggccgc
      481 cagcgttccc tcgcgacccg cctgcaggac ggcgttgtgg aacgccagct gggtgtggac
      541 caggcccata tcgaacagct gctgcccgat ctgcccgtcg ctgctgccca gctgcacgtc
      601 gacggtcacc cgctccgggc tgtcgctggc cggcgtgtcg gccagggcct gcggccggta
      661 ccactccacg aagtcccgca cgatgccgtg ctcgatggag ccggggttct cctccaccca
      721 ggcatacagg cgcggggcga aggcgttgaa ccccaggaag aggaggacgc ccaccagcgc
      781 gatgaccccc gcgaaccagg ccagcagcac caccggctgc gcccggcgcc ggcgccgcag
      841 tggcgcctcg cgctgctcgc ggagctgacg aatgcgggcg ttgcgccgct cgtcgagctc
      901 ccagcgggga tcggtgggta cgctcattca gcctcctgtg cagggtctgg tgcttgtgtc
      961 gggcggggca gggcgtccag gtattcttgc aggatcaggc gggcggccgc cgagtcgagc
     1021 tccccgctcc ggcgttgcgg cctgcggccc gcggccgcca gccgctcgcc ggcctcccag
     1081 ctggtcagcc gctcgtcctg gtaatcgacc gccaggccga tctgggccag gcagtcaccg
     1141 aaggcgcggg cagcggccgc ctgggtaccc tcgctgccgt ccatattgcg cgggaggccg
     1201 acgacgacaa ggctggcacc ctcgctggcc gcaagctccg ccaccgccgc caggtccgcc
     1261 tccaggttcc gcctgcggat ggctggtcgg gcgaaggcca tgccggtctg actgtccccc
     1321 acggccaccc cgatgcggcg gctgccgtgg tcgagaccga tgaagacgct cacccgccgc
     1381 tgacctccac gctgacacgg aacggcaaac gcggcacgcg gtccacccag cccggccaga
     1441 gctccacctc cacctggccg atgtctccca gcgcctggcg cacctccggc tccgtcagtc
     1501 cggtcacccg ctcgcggatg gcgtccgtgt ccaggtcgtg caccgcccgc gccgcgacca
     1561 ccgcctcgac cacgacttcc gcaccctcca ggcgcgcgtc tcccagctca acgctgacgg
     1621 tctccgacag cagggtcgtc cccgcgggcg cggccggatc ggccagcagt tccccacgcg
     1681 ccgcctcctc cgcatcgtcc cgcagaacgt agggccgggc gtaggccagc gtgccggtca
     1741 gcgagaacgt ctcctgtccc tcgctgccga ccaggccgtc cgggatctcc accgccggcc
     1801 gcggtggctc catgggcggg tagatgcgtt ccggctcgcc ggcaaatgcc tcatcccgca
     1861 gccgggtcag ctcggccgcg acctggccgg ccaccgcgtc cacgtcctcc tgcaggacga
     1921 ccgggatgtg ctcctccgag ccgccggcgg tggcggccgg gttgaccacc agccgcctgg
     1981 ggttgttcgg gaagccgcgc aggaagacca tgacgttgcg cgactcgacg gtgtcgatcg
     2041 cctcggccgc cacgttgccg cccggtccgg gttcggccgc cagcaccggg gcgctcgcct
     2101 ctccggcctg gacggtgccc tgtggcgtga actggccgtt tggaacctcg accgtctggg
     2161 tggtgacgaa gcgcgtcccg ccggcgacgg acagctcggt gccctcaggg acctcgacgc
     2221 ggacggtgtt ccagttcagg aaggtgaccg tgccggcggc ggcggtgcgt tccacgcgct
     2281 cgccgctcgc cgcgccctcg cgcgagacct ccagtgtgtc cgtctccggg acctggagcg
     2341 gcggccggac ctcgatggcg cgtggcccga tcggcaccgt gccaggcacg atggtgatgg
     2401 tcgccgaggg tgccgccgcc gcgatggccg caccggccag cagcacgagc gccaccgccg
     2461 cgccgatcaa cgcccgcggc agccgggtgg cacccatccc gcccgccgcg gcacgcggcg
     2521 cgacggcgac cggcgctgca ggcgccagcc gaacggcctg ggtttcctcg gcggctgccg
     2581 ccggagcggc cgctaccggg acggcggagg tggcctcatc cccgcggacg acgtggatcc
     2641 gcgcacggct cggcggaggg gcctcgagtg gcgtcgcgcc gtccgcgctc gcctccgcga
     2701 cggtggcgaa ggaggggatg ccggcctcgc cggccagtcc gcggcccgcg gcatcggcca
     2761 ccagcgccag ctcgcgctcc tcctcggccg cgaccgccgc cagcaggcgc agcgacactg
     2821 cgctgccagc caccttgctg cgtcccgggg cgaccagcac cacccggttg acatcggtct
     2881 cccgcaggcg gcggaccacc gtggtgacct cgtcgtcagg ctccaggtac aggagctgcg
     2941 tgtcgccggc catccgggct acagctgcat ggcgcgcacg gcgctgcgca tggcgcgctg
     3001 cgcgacgccg gcctgggcgt ccagcatcag cgcctggtgg gcgagggcca tgggggtcac
     3061 gtcctgcggc gtccccaggc tgccggtggc gtccagcacg ccggtcacgt cccgcggccc
     3121 gagcgccagc acctgggggc gccgggcgaa gggcagctgc ttccaccatg catcctcggc
     3181 cagcgcggcg gtcacctgcg gcagctccgc gccgccgccg cacaggtaga cccgcgcggg
     3241 cagcagctcg ccctcggcca ggtcggagat catcagctcc acgccacgcc gccagatggc
     3301 ggcgtcctcg gccagggccg cgtccagctc atggcggtcg actgcctcgg gcagctccgc
     3361 ggttccggcg gccagcttca gggcctcggc ccgggcgaac gagaggccga agcgctcggc
     3421 cagccccttg gtgaaggcac ggccccccag cgccagcatc ttggtgccgg cgatcccgcc
     3481 ccggcgcacc agggccacgt cggtcgtgcc acccccgacg tccacgaaca ctgcccccgc
     3541 gtcgccgacc tcccctgggt caagacaggt ggcgaccgcg tagggctcgg cgatcactcc
     3601 cagcaggtgc agccccagtc ggtgcgcgac cgcctgaagc gccccgaggt ggaccatcgg
     3661 cgcgaaggcg ttgaaaacgg ccagctccac ctggctgccg gtgaagccga cggggttgct
     3721 caccgggtag ccgtcgatct tcatctccac gatggccgca tgcaccaggc gcacctccag
     3781 ccgctccacg cccgactccc aggcgatccg ctgctccgct tcgtcgagcg ccgtagcctg
     3841 gacgcgctgc accagcagct cgagctccgg ctcggagagc ggacgctggg cctgatcccg
     3901 ccgcagggtg accgtgctgg tggtgccctt caccagctcg ccggcgatcc cgataacggc
     3961 ggcgctgggt cgcaggccgg cctgggcttc ggcggcgtcc agcgcgaggc ggcagttctc
     4021 gacgacggca tcgatgtcgg ccaccgtccc ggactgcatg tgggtcagcc cctggcgctg
     4081 ccggccgctg ccgcgcacca tgcccccgtt cccgtcgggg ccgacgccca ccaccagcgc
     4141 cttggcgaac tcggtgccga catccagcgc ggtcagcgca ggcggaccgt tgtccgtcct
     4201 ccccgcgacc ggacgccccc gcaggcgtcc caccacctcg ccgaagagct gctgcaccgc
     4261 gcctccgtct ctgccttcgc ggccgccccg cgtcaggccg cctcgatcgg ctcgagggcc
     4321 tcgagcgcca ggcgtgccgc ctcccgcagc gcctcctcga ggcgacccgc gtcccgccca
     4381 ccggcctggg ccagctcggg acgtccgcct ccgcccccgc ccaggatcgc ggcggcggcg
     4441 cgcgcgaccg ccaccgagtc gaacccttcc gccgccaggt cccggctggc ggccaccacc
     4501 agggccgccc cgttgccccc tcccgatgcc accgccacga agcggccggt tacgccgcgc
     4561 agctcatcca cccagccgcg cagcgcgttc gcatcagcct cggggtagtg ctgcacgatc
     4621 acctgcgtct cacctgcctg ctgcgctccc tgtagcgcgg cccgggcatc cgggcgggca
     4681 ccgcccgtcc gcggctcgcg cgccgccttc tccgcctccc gcaaccggtg gctgagcgcc
     4741 tccacccggg ccgggaccgc ctcgtcggcc acgcccagcg cctgcgccgc ctcgcgcagg
     4801 gcggcaaggc gccgggcgac cagctcctcg gccgccgcac cggtgaccgc ctcgatgcgc
     4861 cgcaggccgg cgccgatgct cgactcgccg gtgaccagga agctgccgat ttcgccggtg
     4921 gtcgccacgt gggtgccccc gcacagctcc ttcgagtagc cgtccacgcg caccacccgc
     4981 acccggtcgg gcacgtactt ctcaccgaag aacatgtccg cccccatccg ctgggcctcg
     5041 tccaggctca tccattccgg ttggaccgat gcggccctcc ggatctgctc gttcacccgc
     5101 cgctcgacgt agcgcagcgc ctggcggggc gtggccgccg aggccgggaa gtcgaagcgc
     5161 agcccctccg ggccgaccca gctgcctgcc tgcttggcct gctcccccag cacgtcgcgc
     5221 agggcgcgat gcagcaggtg ggtggccgtg tggttccgcg ccgcggaccg gcgccgatcc
     5281 tcgtcgacca tggcccgcac cacgtcgccg gccgccagct ctccctcaac gcgtcccacg
     5341 tgcacgatgg cctcgccggc gcgctgcgtg tcctcgaccg ccagcttccc gcggggaccg
     5401 atgatctcgc cgcggtcacc gatctggccg ccgccctcgg cgtagaacgg cgtcctgtcg
     5461 aggaccacct gggtggtgcc gtcagccgcg gccacgccca ccacccgggc gacgtcgagc
     5521 gacacctcgt tcggatag
//
LOCUS       DATACA010000488         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2717285, whole genome shotgun sequence.
ACCESSION   DATACA010000488 DATACA010000000
VERSION     DATACA010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717285"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VHK28_09820"
     CDS             complement(<1..311)
                     /locus_tag="VHK28_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX2142548.1"
                     /translation="MGTPDDDYCELHAHSNFSFLDGASHPEELIGRAAQLRMPAVAVT
                     DHAGLYAAVRLWKAAEQTGTDAARDAGLSPVAAIVGLEVAVPRDEGELRLARRGRKLN
                     DP"
     gene            complement(471..1055)
                     /locus_tag="VHK28_09825"
     CDS             complement(471..1055)
                     /locus_tag="VHK28_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142549.1"
                     /translation="MPDPVASSPVRTERLLLRRWRDADRAPFAELNADPRVREHFPGT
                     QSAAQSDASVMQYEAHWAQHGWGPWAVEVPGEAAFIGFIGLSVTDHLGVPIVEVGWRL
                     ERAWWGRGYATEGAGESLRFGFEELGLAEIVSFTVPQNVRSRRVMERIGLVRDAALDF
                     DHPRVDPSAYPHLVRHVVYRQSREDWLRQKVEAG"
     gene            complement(1060..>1725)
                     /locus_tag="VHK28_09830"
     CDS             complement(1060..>1725)
                     /locus_tag="VHK28_09830"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142550.1"
                     /translation="RAPRTPAPSTTEVAGAVAHVPVRRLLAQVIAQPAGRSAVATLVS
                     AQLVMILIMTMTPIHLRGGGHDLGTVGMVISAHTFGMFALSPLSGRAADRFGAPAVIV
                     GGFGVLAAAGLLAAADPSSGRLMALPLFLLGVGWNLCFVAGSSLLASGAAYAERARVQ
                     GGVDSTVWTAGALSSIASGVILDLAGFRTLAIVGSVIAVVLAGAMVVGRGALERAVEP
                     DIP"
BASE COUNT          260 a          651 c          592 g          222 t
ORIGIN      
        1 gggtcgttga gcttgcgacc acggcgggcg aggcgcagct cgccctcgtc ccggggaacg
       61 gccacctcca gtcccacgat ggccgccacc ggggagaggc ccgcgtcccg ggcggcatcg
      121 gtcccggtct gttcggctgc cttccacagg cggaccgcgg cgtacaggcc ggcgtggtcg
      181 gtgaccgcca ccgccggcat gcgcagttgc gcggcccgcc cgatcagctc ctccgggtgg
      241 ctggcaccgt ccaggaagct gaagttggag tgtgcgtgga gctcacagta gtcgtcgtcc
      301 ggcgtcccca tcggacgacc gatgatagaa cgtttgttct atggtgggaa gtggtcgcgg
      361 ccgttgaagc gtccggattc acaccgggtg accaacggat ggccgttcga ccgttggagc
      421 gtcccgattc tcaacgcgca cctggtgtga atccggacaa tgcgccgcgc ctagcccgct
      481 tccaccttct ggcgcagcca gtcctcgcgg ctctggcggt acacgacgtg gcggaccagg
      541 tgcgggtacg cactcgggtc cacccgggga tggtcgaagt cgagcgcggc atcccgcacc
      601 agcccgatgc gctccatcac ccgccgcgag cggacgttct gcgggacggt gaacgagacg
      661 atctccgcca ggcccagctc ctcgaacccg aagcggagcg actccccggc gccctcggtc
      721 gcgtagccgc ggccccacca ggctcgctcc aggcgccagc ctacctccac aatcggtacg
      781 cccaggtggt ctgtcaccga caggccgatg aatccgatga acgccgcctc gccgggcacc
      841 tccaccgccc aaggccccca gccgtgctgc gcccagtgcg cctcgtactg catcaccgac
      901 gcatccgact gcgccgctga ctgtgtcccg gggaagtgct cccgcacccg tgggtcggcg
      961 ttgagctcgg cgaagggcgc ccgatcggca tcgcgccagc gacggaggag caggcgctcg
     1021 gtacgcaccg ggctgctggc gacgggatcg ggcacgagac tacgggatgt ccggctccac
     1081 ggcgcgctcc agcgcgcccc ggccgaccac catggccccg gccagcacca cggcgatgac
     1141 gctgcccacg atggccaggg tgcggaagcc ggccagatcc aggatcacgc cggacgcgat
     1201 gctggagagg gcccccgccg tccagaccgt gctgtccacg ccgccctgga cccgggcccg
     1261 ctccgcatat gccgcgccgc tggccagcag cgacgagccg gccacgaagc acaggttcca
     1321 gcccacgccc agcaggaaga gcggcagcgc catcagccgg ccgctgctcg ggtccgccgc
     1381 cgccagcagc ccggccgcgg ccagcacccc gaacccgccc acgatcaccg cgggcgcgcc
     1441 gaagcggtcc gcggcccgtc cggagagcgg cgagagggcg aacatgccga aggtgtgcgc
     1501 cgagatcacc attccgaccg tgcccaggtc gtgccctccg ccgcgaaggt ggatgggtgt
     1561 catggtcatg atcagaatca tcaccagctg cgcgctgacc agcgtggcca cggcgctgcg
     1621 cccggccggc tgggcgatca cctgcgccag cagcctgcgc accgggacgt gggcgaccgc
     1681 gcccgccacc tcggtggtgg acggcgccgg cgtccgcggt gcccg
//
LOCUS       DATACA010000489         3647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1753051, whole genome shotgun sequence.
ACCESSION   DATACA010000489 DATACA010000000
VERSION     DATACA010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753051"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VHK28_09835"
     CDS             <1..225
                     /locus_tag="VHK28_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HEX2142551.1"
                     /translation="ERVADHARAAGIEIDAFEVQMLYGIRMAEQRRLAAEGYVVKDLI
                     AYGDAWYAWYMRRLAERPANVLFALRQVVG"
     gene            230..1459
                     /locus_tag="VHK28_09840"
     CDS             230..1459
                     /locus_tag="VHK28_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX2142552.1"
                     /translation="MRVLLIGVGTVGEAIARMARDRDWCEGMVLADYDLDRATALQRE
                     LGDARRFPVERIDARDSKAVAELARRHRSNLVMNAVDPRFVMPIFDGALAAGVDYLDM
                     ANSLSRPHPTDPHRQPGLKLADEQLAKAGEWEAAGRLALIGMGMDPGLTDVFAAHAAR
                     NLFDQVDEVHIRDGGDLHIPGYAFAPVFSIWTTIEECLNPPVIWEDGEWRTTEPFSAP
                     EDFPFPEGVGPVECVNVEHEEVLLVPRWIPGVKRVTFKYALGSDFIEKLKVLHDIGLD
                     RVEPIRVRGVEVSPRDVVAAVVPDPITMGDKMRGRAIVGTWVIGRRNGRPREVFLYQM
                     ADAEATLRDTGSQVVGWQTGFNPVLAMELLASGAWSGTGVLAPEAFDPDPYLALMDRY
                     GIHHAMVEMEPGAHQPT"
     gene            1752..2078
                     /locus_tag="VHK28_09845"
     CDS             1752..2078
                     /locus_tag="VHK28_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEX2142553.1"
                     /translation="MNVDEMITGAREAMTVRQVYGDPIERDGLTIIPAAKVRGGGGGG
                     SDTTGNGGGGFGLTARPVGAYIVRNGEVSWEPAVDATRIATMGIVTGIVALLVLRSVV
                     KALTRR"
     gene            complement(2176..2592)
                     /locus_tag="VHK28_09850"
     CDS             complement(2176..2592)
                     /locus_tag="VHK28_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEX2142554.1"
                     /translation="MSIARTVVLVLGAVYVLVGILGFIPAVAEEVAGVDEMDSATALL
                     LGIFPINVLHNFVHLAIGAVLLWGATSTGAAIMAARGVGIVYLLVGLLGFVAPDTFGL
                     MPIGGNDIWLHLATALILLVIGFWPEAATERVDART"
     gene            2805..3530
                     /locus_tag="VHK28_09855"
     CDS             2805..3530
                     /locus_tag="VHK28_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2142555.1"
                     /translation="MTVVDLAFATGQDEGSADAYLVLPAVSRRGPGVLFFHWLEGGSP
                     TSNRTEFLAEARELGGRGVVSLLVDGTFPWRKRPRSAVHDRAAIEAELVMVRRGRDLL
                     ASRPEVDPRRMALVGHDFGAMYSTLLFAEDPRLAGMVMMAPTARWGDWFLEYWQMPDV
                     EADYLRSMAPLDPVAQLRRVGGRPILLQFGSEDEYVPEEVTTEIHRAAGPAARAITYQ
                     AGHQLHAPARADRVAWLEQLLGF"
BASE COUNT          568 a         1230 c         1305 g          544 t
ORIGIN      
        1 gaacgcgtcg ccgaccatgc acgggcggcc gggatcgaga tcgatgcctt cgaggtgcag
       61 atgctgtacg gcatccgcat ggccgagcag cgacgcctgg cggcggaggg ttacgtggtg
      121 aaggacctca tcgcgtacgg cgacgcgtgg tacgcctggt acatgcggcg cctggccgag
      181 cggcccgcga acgtgctctt cgccctgcgc caggtcgtag gctgaccgca tgcgcgtcct
      241 gttgattggc gtcggcacgg tgggcgaggc catcgcccgc atggcccgcg accgcgactg
      301 gtgcgagggc atggtcctcg ccgactacga cctggatcgg gcgaccgccc tccagcgcga
      361 gctgggggat gcgcgccgct tcccggtcga acgcatcgac gcgcgcgaca gcaaggcggt
      421 ggccgagctg gcgcggcgcc atcggtccaa cctggtcatg aacgcggtcg acccgcgctt
      481 cgtgatgccc atcttcgacg gtgccctggc ggccggggtc gactacctgg acatggccaa
      541 cagcctgtcg cgcccgcacc cgaccgatcc gcaccggcag cccggactga agctggcgga
      601 cgagcagctg gccaaggccg gggagtggga ggcggcaggc cggctggcgc tgatcgggat
      661 ggggatggac cccggcctga ccgacgtctt cgccgcgcac gccgccagga acctcttcga
      721 ccaggtggac gaggtgcaca tccgggacgg tggcgacctt cacatccccg gctacgcctt
      781 cgcgccggtg ttcagcatct ggaccaccat cgaggagtgc ctcaacccgc cggtgatctg
      841 ggaggacggc gagtggcgta cgaccgagcc gttcagcgct ccggaggact ttcccttccc
      901 ggagggcgtg ggacccgtgg agtgtgtgaa cgtcgagcac gaggaggtgc tcctggtgcc
      961 gcgttggatc cccggcgtga agcgcgtgac cttcaagtac gcgctgggct ccgacttcat
     1021 cgagaagctc aaggtcctgc acgacatcgg tctggaccgc gtcgagccga tccgggtgcg
     1081 cggcgtggag gtctcaccgc gcgacgttgt ggcggccgtg gtgccggacc cgatcaccat
     1141 gggcgacaag atgcgtggcc gcgcgatcgt ggggacctgg gtcatcgggc gtcgcaacgg
     1201 ccgaccacgg gaggtcttcc tctaccagat ggccgacgcg gaggcgaccc tgcgcgacac
     1261 cggctctcag gtggtgggct ggcagacggg cttcaacccg gtgctggcca tggagctgct
     1321 ggccagcggc gcctggtccg gcacgggcgt gctggcaccg gaggcgttcg accccgatcc
     1381 ctacctggcg ctcatggacc gatacgggat ccaccacgcc atggtcgaga tggagccagg
     1441 ggcgcaccag cccacctgag ccggcgccgg gggtctgccg cggtcgggtg tgccgcggcc
     1501 tgtgggcagc cgccggtggt caccacatgg ctccatcggc caggtagccg cccaggaccg
     1561 cgcctcattg gccgcgggac tcgccgggtg accgtcacgg ccatccagaa gcacgtcgcc
     1621 gcctgttttc cttcgtttcg tgtccgttag agccatccca cggctgcggc acgcgggacc
     1681 aagacgcgtg atcgatgcat cccccacagc ggcctagaat ggactgccgg accgatgcag
     1741 aggtgaacta aatgaacgtc gacgaaatga tcaccggcgc ccgcgaggcg atgacggtca
     1801 ggcaggtcta cggcgacccg atcgagcgcg acgggctcac gatcatcccg gccgccaagg
     1861 tgcgcggcgg cggtggtggt ggcagtgata cgaccggcaa cggcggcggc ggctttgggc
     1921 tgaccgcgcg cccggtcggc gcctacatcg tgcgcaatgg cgaggtcagc tgggagccgg
     1981 ctgtcgacgc gacgcgcatc gccaccatgg gaatcgtgac gggcatcgtg gccctgctgg
     2041 tgctgcgctc agttgtgaag gcgctcaccc gccgctgacc gaggcggtca cgccgcgacg
     2101 ccgaccgagg cggacacgcc gcgacgccga ccgctctccc aagcgatcgg cgtccggcga
     2161 caatcaccct tctgctcagg tcctggcgtc gacccgctcg gtggcggcct ccggccagaa
     2221 gccgatgacc agcaggatca aggcggtggc caggtgcagc cagatgtcgt tcccgccgat
     2281 gggcatcagc ccgaaggtgt ccggtgccac gaagcccagg agaccgacca gcaggtagac
     2341 gatgcccacg ccccgggcgg ccatgatcgc cgcgccggtg gacgtggcac cccacagcag
     2401 gacggcgccg atcgccaggt gcacgaagtt gtgcagcacg ttgatcggga agatgccgag
     2461 cagcagggcg gtggccgaat ccatctcgtc gacgccggcg acttcctccg ccacggcggg
     2521 gatgaacccc aggattccaa cgaggacgta gacggcgccg aggaccagga cgactgtgcg
     2581 cgcgatactc atgggaccct cccttcacga atgatgaacg gctcgggccg taagcaattg
     2641 tggtgccagc cggcaccgcc tgtgaagccc ctcaggcacg ccgagggctc gagtaccaaa
     2701 cggtgctcca tggcaggagt gaacgttcac ggctccgggg cggtaagtcg ggcgtgagcg
     2761 aagcaccgcc gatcaccatc cgatccaccg agagagagaa tggcgtgacc gtggtggacc
     2821 ttgccttcgc cacgggacag gacgagggtt cggccgatgc ctacctggtc ttgcccgccg
     2881 tcagtcgccg cgggcccggc gtgttgttct tccactggct ggagggcggc tcgccgacat
     2941 ccaaccgcac ggaattcctg gccgaggcgc gggagcttgg cgggcgtggc gtcgtctcgc
     3001 tgctggtgga cggcactttt ccgtggcgaa agcgcccacg ttctgccgtg cacgatcgcg
     3061 ccgctatcga ggccgagctg gttatggtcc gccgcggccg tgacctcctc gcgtcccggc
     3121 cggaggtcga cccaaggcgg atggcgctgg tgggacacga tttcggcgcc atgtacagca
     3181 cgctgctgtt cgcggaggac cctcgcctcg cgggcatggt catgatggcc ccgaccgcgc
     3241 gttggggcga ctggttcctc gagtactggc aaatgccgga cgtcgaagcc gattacctgc
     3301 ggtccatggc cccactggac ccggtggcgc agctgcgaag ggtcgggggc cggccgatcc
     3361 tcctccagtt cggctccgag gatgagtacg ttccggagga ggtgaccacc gagatccatc
     3421 gcgcagccgg cccggccgcc cgggcgatca cgtaccaggc cggccaccag ctgcatgccc
     3481 cagcacgcgc cgaccgcgtc gcatggctgg agcagctgct cggcttctag ctggcgggtc
     3541 gggcctcttt gtcggccgcc gcctccgcga tgaagcggct gaccggacgc ggctcgacgc
     3601 ccagcgcccg cagctgactg tcgtccgcgc gcgagggact gcggtcg
//
LOCUS       DATACA010000490         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1433435, whole genome shotgun sequence.
ACCESSION   DATACA010000490 DATACA010000000
VERSION     DATACA010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433435"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..1712)
                     /locus_tag="VHK28_09860"
     CDS             complement(282..1712)
                     /locus_tag="VHK28_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX2142556.1"
                     /translation="MPFDWLSAPDYSISRLLIGRGLAAIYLIAFLVALRQFRPLLGER
                     GLLPTPRYLARTSFRQTPSLFHLGYSDRRLAVVAWVGIVVSATLIIGLPQGAPLPVTM
                     LAWASLWVLYLSIVNVGQTFYAFGWESLLLEAGFLAIFLGNEQTAPPLLTLLLFRWLA
                     FRVELGAGLIKWRGDRCWRDLSCMDFHHQTQPMPNPVSWGFHRLPRRVHRLEVLGNQV
                     AQLAMPFCLLLPQPFAALAAGYMILTQLYLVVSGNYAWLNWVTIIALVAALPDGAFTT
                     LLPVGSPALPEAPAWFHLGVIGLTLLVAYLSWWPVRNLLGSRQLMNYSFNPLHIVNTY
                     GAFGSVTRERDEVVLEGTDAARLDDAAAWRQYEFKGKPGDPRRRPPQVAPYHLRLDWM
                     MWFLPFSPAYAEGWFSRLVEGLLRNDPSVIGLLRSNPFPDQPPRAVRARLYRYRFSTW
                     RERRTSGAWWIREPVGEYLPAVRLEG"
     gene            complement(1725..1801)
                     /locus_tag="VHK28_09865"
     tRNA            complement(1725..1801)
                     /locus_tag="VHK28_09865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1765..1767),aa:Arg,seq:tcg)
     gene            1954..>2858
                     /locus_tag="VHK28_09870"
     CDS             1954..>2858
                     /locus_tag="VHK28_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142557.1"
                     /translation="MPETSFFECGRCGSKTFLGWSTLLGHYCVGCRRYTCQDCWDTTA
                     SCCLDCRPHINSRRPGIPSARRALHQLTRARARLSVLSTGVGGNRAAEQEVLETERRL
                     LAVKSADLSSWIRLAVSDAPQPDDPQSTRILQATDTELAAVEALLAEAEDRSRTRRSR
                     RHGGPGGLDRRWAIALVPVGLLAAWVASSLLGGSMPAGVARESPIASTTTESEVAGGT
                     PAVPRADDGHPPQGSPRVAEAIHLRFDEIITDAPPTDVEFAQAEAVVVPFPNAVDRSV
                     RLTGSGGSGATLCLPVGSRPWTFASV"
BASE COUNT          475 a          969 c          987 g          427 t
ORIGIN      
        1 gactgtgccg cgcattctgg ccgatacgcc ggcaggcggt gtccccggcg acggttgctc
       61 cctcgtttgc aagtggtgat cgcgttagag aacagttcac agtcgatctg tccgcgctac
      121 accccccttt cacagggcgt cgtgctcagt gggtgccgtc tggcgcgcgg tcctttcgtc
      181 acccacctct ctaaggtgac tggcattcgc gggtgagggt cgccgggcct cggtcgggcg
      241 ataacgcgac tggcattcgc gtatgagaac ggcgccgcag ctcacccctc cagccggacg
      301 gctggcaggt actcccccac cggctcgcgg atccaccacg cgccggacgt cctgcgttca
      361 cgccaggtgc tgaaacggta ccgatacagc cgtgcccgca ccgctcgcgg tggctggtcg
      421 gggaacggat tgctgcgcag cagcccgatc accgacggat cgttccgcag caacccctcg
      481 accagccggc tgaaccagcc ctcggcgtac gccggcgaga agggcaggaa ccacatcatc
      541 cagtccaggc gcaggtggta gggcgcgacc tgcggcggcc ggcgtcgggg gtcgccgggc
      601 ttgcccttga actcgtactg gcgccaggcg gcggcgtcgt cgaggcgggc cgcatcggtc
      661 ccttccagga ccacctcgtc gcgctcacgg gtcaccgagc cgaaggcgcc gtaggtgttc
      721 acgatgtgca gcgggttgaa cgagtagttc atgagctggc ggctgccgag caggttgcgc
      781 acgggccacc acgacaggta ggccaccagc aacgtgagac cgatgacccc gaggtggaac
      841 caggccggcg cctcgggcag cgccggcgag ccgaccggaa gcaacgtggt gaaggcgccg
      901 tccggcagcg cggccaccag ggcaatgatg gtcacccagt tcaaccaggc gtagttgccg
      961 ctgaccacca ggtacagctg ggtcaggatc atgtagccgg ccgcgagggc ggcgaacggc
     1021 tggggcagga gcagacagaa cggcatcgcc agctgggcga cctggttacc gagcacctcc
     1081 aggcggtgca cccggcgcgg caggcggtgg aacccccagg acaccggatt gggcatcggc
     1141 tgggtctggt ggtggaagtc catgcagctg aggtcgcgcc agcatcggtc tccgcgccac
     1201 ttgatcaggc ccgcgcccag ctccacccgg aaggcgagcc agcggaacag cagcagcgtg
     1261 agcagcggcg gggccgtctg ctcgttcccc aggaagatgg cgaggaatcc ggcctccagc
     1321 agcagcgatt cccacccgaa ggcgtagaac gtctgcccca cgttcacgat ggacaggtac
     1381 aggacccaca ggctggccca ggccagcatg gtgaccggca aaggcgctcc ctgcggtaga
     1441 ccgatgatca gcgtggccga gacgacgatc cccacccagg ccacgaccgc cagccggcgg
     1501 tcggagtacc cgaggtggaa caggctcggc gtttggcgga atgacgtgcg ggccaagtag
     1561 cgcggcgtgg gcagcagccc gcgttcgccg aggagtggcc ggaactggcg cagtgcgacc
     1621 aggaaggcga tcaggtagat cgccgccaat ccgcggccga tcagcaggcg gctgatcgag
     1681 tagtccggtg ccgacagcca gtcgaagggc atctgggttg aggatggcgc gcccggaggg
     1741 attcgaaccc ccgacctgct gatccgaagt cagctgctct gatccactga gctacgggcg
     1801 cacgttcacg ctcgaagaga gctgataact cgtggcccat tttgccacag cgcttgggca
     1861 ggccggcctg aagggtccag gtaatcgctc aacgtccttg actgccctgc tggttggcca
     1921 tacggtgagc gcaccgtttg ggggaactgg ccggtgcccg agaccagctt cttcgagtgt
     1981 ggtcgctgcg gctcaaagac gtttctgggc tggagcacgc tgctcggcca ctactgcgta
     2041 ggctgccgtc gttacacctg ccaggactgc tgggatacca ccgcttcctg ctgcctcgac
     2101 tgccggccgc acatcaactc ccggcgaccg ggcatcccga gcgcgcggcg cgccctccac
     2161 cagttgacgc gggcacgagc ccgcctcagc gtcctatcca caggggtcgg gggcaaccgc
     2221 gcagccgagc aggaggtcct cgagaccgag cgccgtctgc tggccgtcaa gagcgccgac
     2281 ctgtcgtcgt ggatcaggct ggcggtgtcg gatgcgcctc agcccgacga tcctcaatcc
     2341 acccgcatcc tgcaggcaac cgacacggag ctcgcagcgg tggaggcact actggctgag
     2401 gctgaagatc ggagccgcac cagacggtca cgccgacacg gcgggccggg cggtctcgac
     2461 cgtcgctggg cgatcgccct ggtccccgtc ggcctgctgg cggcgtgggt tgccagctca
     2521 ctgctcgggg ggtcgatgcc ggcgggcgtg gcgcgagagt ccccgattgc atctacaacg
     2581 acggaaagcg aggtcgccgg tggcacccca gccgtgccgc gcgcggacga tgggcaccca
     2641 ccccagggct cgccccgggt ggctgaggcc attcacctgc gcttcgacga gatcatcacc
     2701 gatgccccgc ccaccgacgt cgaattcgcc caggctgagg ccgtggtcgt cccgttccct
     2761 aacgccgtcg atcgcagcgt tcggctgacg ggctcgggcg gcagcggggc gacgctctgc
     2821 ctcccggtcg gctctcgacc atggaccttt gcctccgt
//
LOCUS       DATACA010000491         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2477407, whole genome shotgun sequence.
ACCESSION   DATACA010000491 DATACA010000000
VERSION     DATACA010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477407"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="VHK28_09875"
     CDS             <1..1208
                     /locus_tag="VHK28_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX2142558.1"
                     /translation="AADVDTARQALIKRFGLTEVQANAILEMQLRRLAALERKKIEDE
                     YEQVIRLIAELKRKYGDDRKTRIQSDANRELTAEDLVAAEDVVVTLSQRGYIKRQQIG
                     TFRSQRRGGKGKLAMITREEDAVRHLLVANTHDYILFFTNRGRVFITRVYALPEASRQ
                     AKGLPIINLPGVQVDAGEYVSAIITFPQFEPDHYMVMATKRGMIKKTSLAEYAKIRAN
                     GLIAISLVDGDELRWVGLTDGSDDIILATRKGQAARFHEKEVRPMGRDTRGVTGIRLR
                     TGDEVIGMEIVVPDGELLVVTEQGFGKRTPLPEFPVKHRATGGVIANSLNRDTGDVAA
                     VRVVGREDEEMMLITEEGTILRTQVSSVNRYRRASRGVTVMKPGEGDRIVSIAVFVDD
                     RLPELVLDE"
     gene            1442..2473
                     /locus_tag="VHK28_09880"
     CDS             1442..2473
                     /locus_tag="VHK28_09880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX2142559.1"
                     /translation="MVYADEFSIYAGNANRPLAEKICRYLRTQMGQVEVFQFANENIF
                     VRVLENVREQDVFLVQPTSRPVNQSIMELLIMIDAFKRASAGRITAVIPYFSYGRSDK
                     KDQPRVPITARLIADMLTVAGADRVLTMDLHQGQIQGFFSIPVDELTAVDILCRYIQA
                     KDLSDVVVVSELGFAKKARNFAEKLSAPLAIVEKRRLGNEDRTELMNVIGEVAGKTAV
                     IIDDEIDTAGSLMEAVDALVAEGATEVYSCATHGVFSGAALERIGSSSLKEVIVTDTI
                     PLAAEAPTDRITVLSVAPLFGEAIKRIHRGESVGALFSSEVQLVEEMTFWGSRVDEED
                     EAPAIPIER"
BASE COUNT          540 a          977 c         1012 g          444 t
ORIGIN      
        1 ccgcagccga cgtcgacacc gcccgccagg cgctgatcaa gcgcttcggc ctcaccgagg
       61 tccaggcgaa cgccatcctg gagatgcagc tccgtcggct ggccgcgctc gagcgcaaga
      121 agatcgagga cgagtacgag caggtcatcc gcctgatcgc cgagctgaag cgcaagtacg
      181 gggacgaccg caagacccgt atccagtccg atgccaaccg ggaactgacc gccgaggacc
      241 tggtggcggc cgaggatgtg gtggtcaccc tcagccagcg cggctacatc aagcgccagc
      301 agatcggcac cttccggtcc cagcgccggg gtggcaaggg caagctggcc atgatcaccc
      361 gcgaggagga tgccgtgcgc cacctcctgg tggccaacac gcacgactac atcctgttct
      421 tcaccaaccg cgggcgggtc ttcatcaccc gggtatacgc gctccccgag gcgagccgcc
      481 aggcgaaggg cctgccgatc atcaacctcc ccggcgtgca ggtggatgcc ggcgagtacg
      541 tgagcgccat catcaccttc ccgcagttcg agccggacca ttacatggtg atggccacca
      601 agcgggggat gatcaagaag accagcctgg cggagtacgc caaaatccgg gccaacgggc
      661 tgatcgccat cagcctggtg gacggcgacg agctgcgctg ggtcggcctg accgatggat
      721 cggacgacat catcctggcc acccgcaagg gacaggcggc tcgcttccac gagaaggaag
      781 tgcgacccat gggccgcgac acgcgcggcg tgaccggtat ccgcctgcgc accggcgacg
      841 aggtcatcgg catggagatc gtggtgcccg acggcgagct gctggtcgtc accgaacagg
      901 gcttcggcaa gcggacgccg ctgcccgagt tccccgtcaa gcatcgggcc accggtggcg
      961 tgatcgccaa ctccctgaac agggacaccg gtgacgtggc cgccgtgcgc gtcgtgggcc
     1021 gcgaggacga ggagatgatg ctcatcaccg aggagggcac catcctccgc acgcaggtgt
     1081 ccagcgtgaa ccgatatcgc cgcgcctcgc gcggcgtgac ggtcatgaag ccgggcgagg
     1141 gtgaccgtat cgtgagcatc gccgtcttcg tggacgaccg gctgcctgag ctggtcctgg
     1201 acgaatagcc ggtggctcga ccggcgattg ccggccgccg gccgaacgtg cgcagctgca
     1261 ggctccgctg atgagccgaa ggtgaagaat cggggccaac tacaccgata aggatcgtca
     1321 ctcgcgcaca aggcgaggtc gccagcagag gccgcggtgg ccatcggtca gtccaagggc
     1381 ccagcgccgg gcgtgaggag ggtagaatcg aagctgcgag atcctccgta cgggaccagc
     1441 gatcgtgtac gccgacgagt tcagcatcta cgccggcaac gccaacaggc ccctggccga
     1501 gaagatctgc cgctacctcc ggacccagat gggccaggtg gaggtcttcc agttcgcgaa
     1561 cgagaacatc ttcgtgcgcg tgcttgagaa cgtccgcgag caggacgtct tcctggtgca
     1621 gcccaccagc cggccggtca accagtcgat catggagctg ctgatcatga tcgacgcctt
     1681 caagcgcgcc tctgccggcc gcatcacggc cgtcatcccg tacttcagct acgggcgatc
     1741 ggacaagaag gatcagcctc gggtgccgat cacggcgcgc ctgatcgccg acatgctgac
     1801 cgtggccggc gccgaccggg tcttgaccat ggacctccac cagggacaga tccagggctt
     1861 cttcagcatc cccgtcgacg agctgaccgc ggtcgacatc ctgtgccgct acatccaggc
     1921 caaggacctc tcggatgtgg tggtggtcag cgagctcggc ttcgcaaaga aggcgcgcaa
     1981 ctttgcggag aagctgagcg cgccactggc catcgtcgag aagcggcgcc tggggaatga
     2041 ggatcgcacc gagctgatga acgtcatcgg cgaggtggcc ggcaagacgg cggtcatcat
     2101 cgacgacgag atcgacaccg ccggatcgct gatggaggcc gtggacgccc tggtggccga
     2161 gggagccacc gaggtctaca gctgcgccac gcatggcgtg ttctccggcg cggccctgga
     2221 gcgcatcggc tccagctcgc tcaaggaggt gatcgtgacc gacaccatcc cgctggccgc
     2281 cgaggcgccg actgaccgta tcaccgtcct ctcggtggcg ccattgttcg gtgaggcaat
     2341 caagcgcatc caccgcggtg agagcgtggg cgccctcttc tcgtcagagg tgcagctggt
     2401 cgaggagatg accttctggg gcagccgcgt ggacgaagag gacgaggctc cggccatccc
     2461 catcgagcgc tagccgggtc gctctcgcgg gtgcggtcgc tgcgcatcgc cgtgctggag
     2521 gcgggagggg ccccgagtac gagcccgaca ccatcgggga gcagcccatc ggagggtgag
     2581 cgcgtcggct catgccggca tttgcctcac cggcgcggcg gttcgtccac gaaggtggcg
     2641 attcagccga gcttcgttgg gaaatgtcgt acgggtgcgg cgtaccgatg acgttccggc
     2701 ccaggccggc cactatcgtg ggggttaggc cctaccggta cggcatttcg ggaggatgtg
     2761 gcccgccaca cgcggacatc gtggagaagc tgccgcgcgg gtgcggcacg cgctcacatc
     2821 ggggagttct tcacaggggg ctggtagcgt gtcaatctgc cgctacagcg ggcgcgtccc
     2881 agcacaaagc gacaagctct tccccctcac gctcacggct gcccgatagc gcggcagatc
     2941 agcacgcggc ggcggcttcc cgtgatcctc cgc
//
LOCUS       DATACA010000492        14702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2076564, whole genome shotgun sequence.
ACCESSION   DATACA010000492 DATACA010000000
VERSION     DATACA010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14702
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076564"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHK28_09885"
     CDS             complement(<1..350)
                     /locus_tag="VHK28_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX2142560.1"
                     /translation="MSSVTAMATFVEPESGMRVDARFDSGTVAIYDVLEGQDRAGPSP
                     TESLLGALAACTAMDVASILRKKRQVVRTYEIAVVGQKSDEHPKVYTSIVVEHRVAGM
                     VDAEALRRSVELSAT"
     gene            380..1120
                     /locus_tag="VHK28_09890"
     CDS             380..1120
                     /locus_tag="VHK28_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142561.1"
                     /translation="MRVIRPAPPARSWDLALSLGMLLVGLAAILLMALLPGERPGVAA
                     LLGLAAALGLGVGLAWLIRTVSDHRQPPGVDIIRMLDGAFDDSYVLVISPRLPDVPHD
                     LAALLVGPAGVRALIARRWRGRYRVRGRGWEYDTKSAEGWIPCRTNPSFDADAAAEAV
                     ARWARTAADEPGLPLAGAIAFPRAFSTLVLEEPESEIVTTDNAPWWAQRIGRVQRLDP
                     QRAARFVQAVLDAADARAAPAREPVTTG"
     gene            complement(1140..1760)
                     /locus_tag="VHK28_09895"
     CDS             complement(1140..1760)
                     /locus_tag="VHK28_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142562.1"
                     /translation="MTTPEFGLDPFAPSATRNVALDVYTSAYRVSGQLATRFNRIADI
                     VNQVTASHLVIEHATVSEYADPTATLGALQILVSLDDVLFVVGGETDGAARPEMRIPK
                     RAVRAQMGLPPFRLTGMVHVPQGSRPVDGLLNVGERFVPMTQVTVACGAHSELGRTAD
                     AVALQRRLAHLILVTDDEHPDELLAEVLDGATAERWLAESREPESF"
     gene            complement(1786..2562)
                     /locus_tag="VHK28_09900"
     CDS             complement(1786..2562)
                     /locus_tag="VHK28_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2142563.1"
                     /translation="MTTEELPEHVRRNREYWNELAPQYVAHGEESWATGEARWGIWGI
                     PDSEVGLLPAELEGRDAIELGCGTAYVSAWLARRGARPTGIDNSPAQLETARRLQREH
                     GLEFPLLLGNAEELPFPDATFDLAISEYGAALWADPYRWVPEAARVLRPGGELVFLTN
                     GLICMLCVPDTEAEGPATDRLRRPYFGMHRNEWPDTDGIELHLGYGDWIRLLRRSGFE
                     VLDLVELRPPPGATTSYEWVTADWARQWPSEEAWKARRVG"
     gene            complement(2582..3430)
                     /locus_tag="VHK28_09905"
     CDS             complement(2582..3430)
                     /locus_tag="VHK28_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2142564.1"
                     /translation="MFDGFVMERRRVGDVELRVRIGGSGRPLLLLHGHPQTHAMWHLV
                     APSLAERHTVVVPDLPGYGESTPIGSGSIDGSKRAMARVMVALMASLGHQRFDLAGHD
                     RGGRVGYRLALDDPERIRRLAVLDIVPTAEMWRRMNTSLAMANWHWLFLAQAAPMPER
                     LLSADPEAYYYRDRRERFHPEALADYLAACRRPEVIHAMCQDYRAGATLDRQLDEADA
                     AAGRRIAAPLLALWSAEGEIANWDPIQVWRAWAEDVTGRELACGHYLAEEAPDVVIAE
                     LRRFLG"
     gene            complement(3434..5167)
                     /locus_tag="VHK28_09910"
     CDS             complement(3434..5167)
                     /locus_tag="VHK28_09910"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2142565.1"
                     /translation="MSKSSPFADFARTADALAATRSKLAKRDLLAAYLRDLPDGDLPV
                     AATFFAGRPLPGADDRLGLGWVQQSQALAAASGADAEALNAAYLRHSDFGDAAFELLE
                     RRAPSGRRLTLADVDAAFRALSGAPGAEARVTEMARLFGRATAAEARYVGRIASRELR
                     IGLREGLLEEAIAAAFERPIADVRRALMLVGEPGEAAVLARQDTLGDAALHLGRPVRF
                     MLATPVPDAAEVMRRVGDEAWIEDKYDGIRAQLHVDVAGGLPPRLFSRDLREVTRSFP
                     DVIGPLAGLGHALVVDGELVPYRAGSVLDFASLQTRLGRVNPSAQLLAEVPVVLVAFD
                     LLHLDGRDLLEVPLRERRAALEGLELPALTGEQVLYSHLATARSADEVETHFDDARER
                     RNEGLMIKDPASVYQPGRRGLGWLKLKKALATLDCVVVGVEWGHGKRRGVLSDYTFAV
                     RASDEPDASLLTIGKAYTGLTDAEIAIMTEHFHSITLRDFGRYRTVVPEVVVEIAFDR
                     IMRSNRHKSGFAMRFPRIVRIREDKTPAEIDTLATVEALFGEQASGRILLATGERGET
                     ERIAATGEVGE"
     gene            5228..5836
                     /locus_tag="VHK28_09915"
     CDS             5228..5836
                     /locus_tag="VHK28_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX2142566.1"
                     /translation="MVFKWQRPSDDPEIAKRIPPGQVLTDKFPVLHFGRVPDYPDLSR
                     WDFRVFGDVETPFTLGWTQFRQLPVTTVTQDVHCVTRWSKLDTTWEGVPFSHLVDVAQ
                     PRPEARFVIFHAEAGFTSNVPLEVAMDSLCVLAWSYAGQPLTPEHGYPLRAIVPGKYF
                     WKSAKWLRGVEFSTEDRPGFWERNGYSNSADPWREERYADGS"
     gene            complement(5843..6100)
                     /locus_tag="VHK28_09920"
     CDS             complement(5843..6100)
                     /locus_tag="VHK28_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142567.1"
                     /translation="MAGEEKQERKQEPMSVRDAGRKGGKATRQRYGPEFFAEIGQKGG
                     RAVSQRYPSEHFQEIGRKGGRRVAELVERAKQLEGESENGK"
     gene            6224..6532
                     /locus_tag="VHK28_09925"
     CDS             6224..6532
                     /locus_tag="VHK28_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142568.1"
                     /translation="MHPLLRNIVIGIVGLLIAGGLTTLAVLGEDSSTSVLAMLGAGLL
                     SVAVGIFLFIQGWIWSQRMWRRGSAAASVAVAIGGGLMIVLAAVALAGTVILLLLFYI
                     "
     gene            6602..6678
                     /locus_tag="VHK28_09930"
     tRNA            6602..6678
                     /locus_tag="VHK28_09930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6636..6638,aa:Glu,seq:ttc)
     gene            complement(6683..6862)
                     /locus_tag="VHK28_09935"
     CDS             complement(6683..6862)
                     /locus_tag="VHK28_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142569.1"
                     /translation="MGRQNVPRDQWLEIGAECPHCGERIGEEMVYSWRPMPEDPKLLL
                     LYCPDCGDRVEINHV"
     gene            complement(6862..7395)
                     /locus_tag="VHK28_09940"
     CDS             complement(6862..7395)
                     /locus_tag="VHK28_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142570.1"
                     /translation="MISSLIRTFRGPSRDPRMEYISRPLPKGLTDEEVVAALESALEA
                     NPDPGFLVETLPGALREVTGREYQVLDRSVRDVTGAFSKTSVMIRDGDVGLWPGYAAR
                     AYPLLKPSAKAEETRRAIAAAEGAEAAEPSPTNPVGKPGWAPPPPVGRAPPPREPIGT
                     AIAGSGENRDEAFEEPA"
     gene            7577..8449
                     /gene="galU"
                     /locus_tag="VHK28_09945"
     CDS             7577..8449
                     /gene="galU"
                     /locus_tag="VHK28_09945"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX2142571.1"
                     /translation="MRVRKAVLPAAGFGTRFLPATKAIPKEVMPLVDRPIIQYAVEEA
                     VASGIEQIVIVIATGRSAIEDHFDANPTLERWLEERGDIEMLRAVRRITEVGPIAFVH
                     QKEQLGLGHAVLMAKELVGEEPFAVLLSDDVMLNPGGEPVLRQLIGQYQAHRGSVVAV
                     QRVPKRDVTRYGIVRPVREEGRLVEIGDLVEKPSETDAPSDLAVLGRYVLTPKIFDVL
                     EATPRGAGGEIQLTDAIRALTGEQPVFGYQFEGVRHDAGTRLGWLEANVAMALESDDA
                     DEFRTYVRGLDLRD"
     gene            8502..10157
                     /gene="pknB"
                     /locus_tag="VHK28_09950"
     CDS             8502..10157
                     /gene="pknB"
                     /locus_tag="VHK28_09950"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEX2142572.1"
                     /translation="MIGRLINDRYRLIAPLGEGGMATLWRAVDQQLDREVAVKILREQ
                     YGSDAGFAARFRQEARSAGSLSHPNIVPVYDYGTDAEVGTQFIVMQLVEGQDLAAVLQ
                     ERGTLSTDDAVRVALAVASALEAAHRRGIVHRDVKPGNILITDDGEVKVTDFGIARAV
                     SEASMTVTGTTLGSVHYFSPEQARGDEVTGRSDVYALGIVLYEMLTGRRPFEGDSAAG
                     VALKRLTEDPPPPSSFRPVPSGLEAIVMRALQREPGDRFPDAGSFAEALRAWQRDPAA
                     GAAAAATGAAAGTSAPPPAPLPPAGEPTMYVPPPVARPSDRAPMNVNAPPPRRPAPRY
                     EESRGQPWWMWLLLLLAVILLGTIGFLGAQILGNLGPGATPSPSANVVEVPNWVGEPI
                     VAVRDEAEELGLDLDEDEEPSDDVEEGRVISTNPAAGEPVAEGGTVVVVVSSGADQVQ
                     VPSLIGQTEEQAAQTLRNAELELGTVTREFSEEPEGRVIRSNPAAGVDWPRGDQVDIV
                     VSRGPEPTPTPSPTPEPTVAPTATPEPPPPPTATPTPTPAATP"
     gene            10610..10924
                     /gene="trxA"
                     /locus_tag="VHK28_09955"
     CDS             10610..10924
                     /gene="trxA"
                     /locus_tag="VHK28_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2142573.1"
                     /translation="MAVDESTFEAQVLSADKPVLVDFWAPWCGPCRLVSPVVESVGEA
                     HADKIGVAKVNTDENQQLAMRYSIFSIPTLILFKDGREAARLVGYMPQEAMEDRLRDF
                     LA"
     gene            11057..11614
                     /locus_tag="VHK28_09960"
     CDS             11057..11614
                     /locus_tag="VHK28_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142574.1"
                     /translation="MIIKTERRSGADRRQTEDPFAQERRGPDRRTMCWEVENCSLTIR
                     YRCPAFILRRPCWELWAVDGVGPARACCHNEIDCEPGRCVIAEKRFATSPEPLTVHVG
                     RRGVPLGYARPRRHSCPNFFLTQGAKGRVPIEAPQLLRTLMKQEGVVSRCQVRPGVHL
                     DQGYVTDLCLTGQFHDCVFYEDESA"
     gene            11637..12983
                     /locus_tag="VHK28_09965"
     CDS             11637..12983
                     /locus_tag="VHK28_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX2142575.1"
                     /translation="MTTALAYAAAVRELRERGRFGIGLGLERVTAILDELGHPERALH
                     GALVAGTNGKGSVVALARSALAAACLRTGTMPKPHLVSYRERIAIDGVPLSEDRFAAA
                     LASVLPAVDRVAARLGPPTEFEVLTAAAYAELARAGVDLALVEVGLGGRLDATNVLDP
                     GVAVITNVQHDHERHLGRTLAAIGAEKAAIIKRGNLAVTGATGRGLRPILDRCAALDV
                     PLRRAGSGQPYAVEVRHVGWDGLVVDARTPSGWTRDLHVRLLGRHQASNAAVALALLD
                     ALRDDGERRGKPLLGLDEAAVREGFERVSWPGRMELLPTTRLGRVLLDGAHNPAGARA
                     LADALDELGLRSFPLVFGAMRGKRVSAVLRALAPLRPNPVFTQVEGGAAIRSRTLQAA
                     WRRITGSGGQSTAPPAAALELAATLRQDAGQPIVVAGSLYLVGDIRGMLRPQEDGA"
     gene            12980..13834
                     /gene="folP"
                     /locus_tag="VHK28_09970"
     CDS             12980..13834
                     /gene="folP"
                     /locus_tag="VHK28_09970"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2142576.1"
                     /translation="MTELRWGERTYVMGIINVTPDSFSGDGLAEPGASELEIVAAALQ
                     QARAFVAAGADILDVGAESSRPAQVYGEHPPIDVEAEIARAVPVVAALSREFAGRTLV
                     SIDTMKGAVAREALAAGAGMVNDVWAGRHDPGTVDAAAAAGAYLVLMHNKEAAEYPGG
                     MFGEVVAWLRSAVDDAVDRGMPRERLLVDPGIGFGKKPAHSVEVLHRLAELKDALGGL
                     PLLVGTSRKRFIGEILGGVPPQERVEGTAATVALAIAAGADVVRVHDVAHMVRTARVS
                     DAIVRLRQ"
     gene            13831..14214
                     /gene="folB"
                     /locus_tag="VHK28_09975"
     CDS             13831..14214
                     /gene="folB"
                     /locus_tag="VHK28_09975"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX2142577.1"
                     /translation="MSVDDRITLRGMRFLGRHGVTLEERMEPQPIEVDLILRRDLSKA
                     VASDELADTTDYSAVFTLVGAVVEGQSFRLLEALAGAIADSVRTEHAVDDVEVRVRKP
                     QAPLPGTLETVEVRVRRAVAAREKG"
     gene            complement(14211..>14702)
                     /locus_tag="VHK28_09980"
     CDS             complement(14211..>14702)
                     /locus_tag="VHK28_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX2142578.1"
                     /translation="NQGNSGGPLIDSAGQVIGINTAVNEDAQGIGFAIPIDVAKPILS
                     QALEGVEIARPWIGVFYQPVTQQLAEDQDLPVDYGVLVESGNPNRPAVFPDSPAAAAG
                     LEAGDVIVAVDGQRVDAEDDLAEHILPHRPGDEIVLRVLRGGGGGSTREVTVTLGTLP
                     EQP"
BASE COUNT         2047 a         5330 c         5226 g         2099 t
ORIGIN      
        1 gtggccgaca gctcgaccga gcgccgcagc gcctccgcgt ccaccatccc cgccacccgg
       61 tgctcgacca cgatcgaggt gtagaccttg gggtgctcat cggacttctg tccgaccacc
      121 gcgatctcgt aggtgcgcac cacctggcgc ttcttgcgaa ggatgctggc cacgtccatg
      181 gcggtgcagg ccgccagcgc cccgagaaga gactccgtgg gagacgggcc ggcacgatcc
      241 tggccctcca ggacgtcgta gatcgccacc gtgccggagt cgaaccgcgc gtccacgcgc
      301 atgccggatt cgggttcgac gaaggtggcc atggccgtga cgctgctcac ttgccgagcg
      361 tagcagcagg ggtggaccga tgcgcgtgat ccggccggcg ccgccggcgc gatcgtggga
      421 cctggcgctc tcgctgggca tgctcctggt ggggctggcc gccatcctcc tgatggcgct
      481 gctgcccggc gagcggccgg gcgtggcggc cctgctgggc ctcgcggccg ccctgggcct
      541 gggagtgggg ctggcgtggc tgatccgaac ggtcagcgac caccgccagc cgccgggcgt
      601 ggacatcatc cgcatgctgg acggcgcctt cgacgacagc tatgtgctgg tcatcagccc
      661 gcggctgccg gacgtgccgc acgacctggc cgcgctgctt gtcggaccgg ccggcgtgcg
      721 ggccctgatc gcgcgccgct ggcgcggccg ctatcgggtg cggggtcgcg gatgggagta
      781 cgacaccaag tcggcggagg gctggatccc ctgccgcacc aacccgtcgt tcgacgccga
      841 cgccgctgca gaggcggtgg ccaggtgggc gcgcacagcc gccgatgagc ccggcctgcc
      901 cctcgccggg gcgatcgcct tcccgcgggc gttcagcaca ctggtcctcg aggagccgga
      961 gagcgagatc gtgaccaccg acaacgcgcc ctggtgggcg cagcgcatcg ggagggtgca
     1021 gcgcctggac ccgcagcgcg ccgcgcgctt cgtgcaggcg gtgctggacg ccgcagatgc
     1081 cagggcggct ccggcccggg agccggtcac caccggctag cggcagggcg cggctccgct
     1141 cagaacgact ccggctcgcg cgactcggcc agccagcgct cggcggtcgc gccgtcgagc
     1201 acctctgcca gcagctcgtc ggggtgctcg tcatcggtca ccaggatcag gtgcgccagc
     1261 cgccgctgca gcgccactgc gtctgccgtg cggcccagct ccgaatgcgc gccgcaggcc
     1321 accgtgacct gggtcattgg cacgaagcgc tcccccacgt tgagcagccc gtccaccggc
     1381 cggctgccct gtggcacgtg caccatgccg gtcagccgga acggcggcag gcccatctgc
     1441 gcgcggaccg ctcgcttggg gatgcgcatc tcgggcctgg cggctccatc ggtctcaccc
     1501 ccgaccacga acagcacgtc gtcgagcgag accaggatct ggagcgcacc cagggtggcg
     1561 gtcgggtccg cgtactcgct gaccgtggcg tgctcgatca caaggtggct ggcggtcacc
     1621 tggttgacga tgtcggcgat gcgattgaag cgggtcgcca gctgcccgga gacgcggtag
     1681 gcggaggtgt agacgtccag cgccacgttc cgcgtggcgg acggggcgaa cgggtccagg
     1741 ccgaactcgg gtgtggtcat ggctggaccg atgctaccgc gcgcatcaac ccacgcggcg
     1801 cgccttccac gcctcctcgc tcggccactg gcgcgcccag tcggcggtga cccactcgta
     1861 cgaagtggtg gcgcccggcg gcgggcgcag ctcgaccagg tcgagcacct cgaacccgga
     1921 gcgccgcagg agccggatcc agtcgccgta gcccaggtgg agctcaatgc cgtcggtgtc
     1981 cggccactcg ttgcggtgca tcccgaagta cgggcggcga aggcggtcgg tggccgggcc
     2041 ctcggcctcg gtgtccggca cgcacagcat gcaaatcagc ccattggtca ggaacaccag
     2101 ctcgccgccc ggcctgagga cgcgcgccgc ctcgggcacc cagcggtagg gatcggccca
     2161 cagcgcggcg ccgtactcgc tgatggcgag gtcgaaggtg gcgtccggga acggcagctc
     2221 ctcggcgttg cccagcagca gcgggaactc caggccgtgc tcgcgctgca gccgacgcgc
     2281 cgtctcgagc tgcgccggcg agttgtcgat cccggtcgga cgcgccccgc gccgcgccag
     2341 ccaggcggag acataggccg tgccgcagcc cagctcgatc gcgtcccgcc cctccagctc
     2401 cgccggcagc agccccacct ccgagtcggg gatgccccag atgccccagc gcgcctcgcc
     2461 ggtggcccag ctctcctccc cgtgggcgac gtactgcggc gccagctcgt tccagtactc
     2521 gcggttgcgc cggacgtgct cgggaagctc ctcggttgtc acgcggccga tgctaccccg
     2581 ctcagcccag gaagcgccgc agctcggcga tcaccacgtc gggcgcctcc tcggccaggt
     2641 agtggccgca ggccagctcg cgcccggtga cgtcctccgc ccacgcccgc cagacctgga
     2701 tcgggtccca gttggcgatc tcgccctcgg cgctccacag cgccagcagc ggtgcggcga
     2761 tacgccggcc ggccgcggca tcggcctcgt ccagctggcg gtcgagggtg gcacccgcgc
     2821 ggtagtcctg gcacatggcg tggatcacct ccgggcggcg gcaggcagcc aggtagtcgg
     2881 ccagcgcctc ggggtggaag cgttcgcggc ggtcccggta gtagtacgcc tccggatcgg
     2941 ccgacagcag ccgttccggc atgggagccg cctgcgccag gaagagccag tgccagttgg
     3001 ccatggccag cgaggtgttc atgcgacgcc acatctcggc ggtcggcacg atgtccagga
     3061 ctgccagccg tcgaatccgc tccgggtcgt cgagcgccag gcggtagccc acccggccgc
     3121 cgcggtcgtg cccggccagg tcgaagcgct ggtggccgag ggatgccatc agcgccacca
     3181 tcacgcgcgc catggcccgt ttcgagccgt cgatcgatcc gctcccgatc ggcgtcgact
     3241 cgccgtagcc cggcaggtcg ggcacgacca cggtgtgccg ctcggcgagc gacggcgcca
     3301 ccaggtgcca catggcgtgc gtctgcggat ggccgtgcag cagcagcagt ggccgaccgg
     3361 agccgccaat tcgcacgcgc agctccacgt cccctacgcg ccgccgctcc atgacgaacc
     3421 cgtcgaacac ggcctactca cccacctcgc cggtggccgc gatgcgctcc gtctcgcccc
     3481 gctcgccggt ggccagcagg atgcgtccgg aggcctgctc cccgaacagg gcctccaccg
     3541 tggccagcgt gtcgatctcg gccggcgtct tgtcttcccg gatcctcacg atccgcggga
     3601 agcgcatggc gaagccggac ttgtggcggt tggagcgcat gatgcggtcg aacgcgatct
     3661 cgaccaccac ctccggcacc accgtccggt agcgcccgaa gtcgcgcagg gtgatcgagt
     3721 ggaagtgctc ggtcatgatc gcgatctccg catcggtcaa tccggtgtag gccttgccaa
     3781 tggtcagcag cgacgcgtcc ggctcgtcgc tggcacgcac cgcgaaggtg tagtcgctca
     3841 gcacgccccg ccgcttgccg tggccccact ccacgccgac gaccacgcag tccagggtgg
     3901 ccagcgcctt cttcagcttc agccagccaa gtccccggcg tcccggctgg tagaccgatg
     3961 ccgggtcctt gatcatcagc ccctcgttgc gccgctccct ggcgtcgtcg aagtgcgtct
     4021 cgacctcatc cgctgagcgg gccgtggcca ggtgcgagta cagcacctgc tcccccgtca
     4081 gcgccggcag ctccagcccc tcgagcgccg cccgccgctc ccgcagtggc acctccagca
     4141 ggtcgcgccc gtccaggtgc agcaggtcga aggcaaccag caccaccggc acctcggcga
     4201 gcagctgggc ggacgggttc acccgcccca ggcgggtctg caggctggcg aagtccagga
     4261 ccgatccggc gcggtacggc accagctcgc cgtccaccac cagggcgtgc cccaaccctg
     4321 ccagtggacc gatgacgtcc ggaaacgacc tggtcacctc gcgcaggtcg cgactgaaca
     4381 ggcgcggcgg caggccgccg gccacgtcca cgtgcagctg ggcccggatg ccgtcgtact
     4441 tgtcctcgat ccaggcctcg tcacccaccc ggcgcatgac ctcggccgcg tcgggcaccg
     4501 gcgtggccag catgaagcgg accgggcgcc ccaggtgcag ggccgcatcg ccaagcgtgt
     4561 cctggcgcgc gaggacggcc gcctcacccg gctcgccgac cagcatcagc gcgcggcgca
     4621 cgtccgcgat cggccgctcg aaggcggccg cgatggcctc ctccagcagc ccctcccgca
     4681 gcccgatgcg cagctcgcgc gaggcgatgc gcccgacgta gcgcgcctcg gcggccgtcg
     4741 cccggccgaa gaggcgggcc atctcggtca cccgcgcctc ggcgccgggt gccccgctca
     4801 gcgcccggaa cgcggcgtcc acgtcggcga gggtcaaccg cctcccagac ggtgcgcgcc
     4861 tttcgagcag ctcgaacgcg gcgtccccga agtcggagtg gcgcaggtag gccgcgttga
     4921 gcgcctctgc atcggcgccg gaggcggcgg ccagggcctg gctctgctgc acccagccca
     4981 gccccaggcg gtcatcggcc ccgggcaggg ggcggccggc gaagaaggtg gcggcgacgg
     5041 gcaggtcgcc atcgggcagg tcgcgcaggt aggcggccag caggtcgcgc ttggccagct
     5101 tggagcgcgt ggcggccagc gcatcggcgg tgcgcgcgaa gtcggcgaag ggggaggact
     5161 tgctcacgac gcgcggcatg gtcgcatgcc ttgacgccca cccatcggtc catcagcatc
     5221 cgagatcatg gtcttcaagt ggcagcgtcc ctctgacgac ccggagattg ccaagcgcat
     5281 cccgcccggc caggtgctga ccgacaagtt cccggtcctg cacttcgggc gggtgccgga
     5341 ctaccccgac ctgtcacgct gggatttccg cgtgttcggc gacgtggaga cgcccttcac
     5401 cctgggctgg acccagtttc ggcagctgcc ggtcaccacc gtgacccagg acgtccactg
     5461 cgtcacgcgc tggtcgaagc tcgacacgac atgggagggc gtgcccttca gccatctcgt
     5521 cgatgtcgcg cagccccgcc cggaggcgcg cttcgtgatc ttccacgccg aggccggctt
     5581 cacctccaac gtgccgcttg aagtggccat ggactccctg tgcgtgctgg cctggtccta
     5641 cgccgggcag cccctcaccc cggagcacgg ctacccgctg cgcgccatcg tgccgggcaa
     5701 gtacttctgg aagagcgcca agtggctccg gggcgtcgag ttcagcacgg aggacaggcc
     5761 ggggttctgg gagcggaacg gatactcgaa ctcggccgac ccgtggcgcg aggagcggta
     5821 cgcggacggc tcctgagcac ggctacttcc cgttctcgct ctcgccctcc agctgcttgg
     5881 cgcgttccac cagctccgca acgcgtcggc cgcccttgcg cccgatctcc tggaagtgct
     5941 ccgaagggta gcgctggctg actgcccgcc cgcccttctg cccgatctcg gcgaagaact
     6001 ccgggccgta gcgctggcgc gtggccttcc cgcccttccg tccggcatcg cggacgctca
     6061 tgggctcctg cttgcgttcc tgcttctctt ctccggccat cacggctcct agctcccaac
     6121 cctggtcgta tggcgtgtca ttgcacgctc ggcctgcgag cagccggagt gcaggatcgg
     6181 tgccgaacgt gcgaccgggg ccggcggcta gaatgcgccg gccatgcacc cgttgctccg
     6241 caacatcgtg atcgggatcg tgggcctgct catagccggc gggctgacga cgctcgccgt
     6301 gctcggagaa gactcgtcca catcggtcct ggccatgctc ggcgccgggc tgctgtcggt
     6361 cgcggtgggc atcttcctgt tcatccaggg ctggatctgg tcgcagcgaa tgtggcgccg
     6421 gggttcggca gctgcctcgg tggcggtggc catcggtggc gggctgatga tcgtgctcgc
     6481 cgccgtggcc ctggccggca cggtcatcct gctgctgctg ttctacatct gaccgggccg
     6541 atggcgccct ccgggcgcct tgctaccatt gagcgcgccg catctcggtc gtgcgccgga
     6601 cgcccccatc gtctagaggc ctaggacgcc accctttcaa ggtggagatc accggttcga
     6661 atccggttgg gggcaccacc cctcagacgt ggttgatctc cacgcggtcg ccgcagtccg
     6721 ggcagtagag gagcagcagc ttggggtcct ccggcatcgg ccgccaggag tagaccatct
     6781 cctccccgat gcgctcgccg cagtgcgggc actcggcgcc gatctccagc cactggtcgc
     6841 gcgggacgtt ctggcggccc atcaggccgg ctcctcgaag gcctcgtcgc ggttctcgcc
     6901 cgagccggcg atggcggtgc cgatcggctc acgcggcggt ggggcgcgac caacgggcgg
     6961 cggcggcgcc cagccgggct tgccgaccgg gtttgtgggc gacggctctg cggcctcggc
     7021 accctcggcg gcggcgatcg cccgccgcgt ctcctcggcc ttggccgacg gcttgagcag
     7081 gggataggcg cgcgccgcgt agccgggcca cagtcccacg tccccgtccc ggatcatgac
     7141 cgacgtcttg ctgaaggccc cggtcacgtc gcgcaccgaa cggtcgagca cctggtactc
     7201 gcgcccggtc acctcgcgca gcgcgcctgg cagcgtctcc accaggaagc cgggatcggg
     7261 gttcgcctcc agtgcgctct ccagcgcggc gacgacctcc tcgtcggtca gccccttggg
     7321 caacgggcgg ctgatgtact ccatgcgcgg gtcgcgcgag gggccgcgga aggtgcgaat
     7381 caaggagctg atcatcgggt caccgattgt agcgccgggt gctccgggga gtgtgcggcc
     7441 cggcggttgc tatacttcac ggcccgtgat ggaccctcgc atccgcgctg cgcgtcccgc
     7501 ctgaccgggg ccgccgcagc tggaccaccc gcccatcaga gcggtccgaa ccagcaacag
     7561 gagacacctc atcaccgtgc gcgtcagaaa ggccgtcttg ccggcggccg gcttcggaac
     7621 tcgcttcctc cccgcgacca aggccatccc caaggaggtg atgcctctgg tcgaccggcc
     7681 catcatccag tacgccgtcg aggaggcggt ggcatccggc atcgagcaga tcgtgatcgt
     7741 gatcgccacc gggcgcagcg ccatcgagga ccacttcgac gccaatccca cgctggagcg
     7801 ctggcttgag gagcgtggcg acatcgagat gctgcgcgcc gtgcgccgca tcaccgaggt
     7861 cggccccatc gccttcgtgc accagaagga gcagctgggg ctgggacacg cggtgctgat
     7921 ggccaaggag ctggtcggcg aggagccatt cgcggtcctg ctctccgacg acgtcatgct
     7981 caaccccggt ggagagccgg tcctccgcca gctcatcggc cagtaccagg ctcaccgcgg
     8041 gagcgtggtg gccgttcagc gcgtgccgaa gcgtgacgtc acgcgctacg ggatcgtcag
     8101 accggtacgc gaggaggggc gcctggtgga gatcggcgac ctggtggaga agccttcgga
     8161 gactgatgcg ccgtcggacc tggcggtcct cggccggtac gtgctgaccc ccaagatctt
     8221 cgacgtgctg gaggccacgc cacgtggcgc cggcggcgag atccagctca ccgacgccat
     8281 ccgggcgctg accggcgagc agccggtctt cggctaccag ttcgagggcg tgcgccacga
     8341 cgccgggaca cgcctgggct ggctggaggc caacgtggcc atggcgctcg agtcggacga
     8401 tgccgacgag ttccggacgt acgtccgggg gctggacctg cgagactgat cccgccgacg
     8461 gcgcacacgg cagcggaaca cggagggcga cgcgtgtgga gatgatcggg cggctgatca
     8521 acgaccgata ccggctgatc gccccccttg gcgagggcgg catggccacc ctgtggcggg
     8581 ccgtcgacca gcagctggac cgcgaggtcg ccgtcaagat cctgcgcgag cagtacggca
     8641 gcgatgccgg gttcgccgcc cgcttccgcc aggaggcccg ctccgccggc tccctgtcgc
     8701 accccaacat cgtgccggtc tacgactacg ggaccgatgc cgaggtcggc acccagttca
     8761 tagtcatgca gctggtggag gggcaggacc tggccgccgt cctccaggag cgcggcacgc
     8821 tgtcgaccga tgacgccgtg cgggtggccc tcgccgtggc atcggccctc gaggcggcgc
     8881 atcggcgggg catcgtccac cgtgacgtga agcccgggaa catcctgatc accgatgacg
     8941 gcgaggtgaa ggtcaccgac ttcggcatcg cgcgcgccgt gtccgaggcc tccatgacgg
     9001 tcaccggcac cacgctcgga tcggtccact acttcagccc ggagcaggcg cgtggggacg
     9061 aggtgaccgg ccgctccgac gtgtatgcgc tgggcatcgt cctgtacgag atgctgaccg
     9121 gccggcgccc gttcgagggc gactcggcgg ccggcgtggc cctcaagcgg ctgaccgagg
     9181 atccgccgcc gccatcgtcc ttccggccgg tcccctcggg cctggaggcg atcgtgatgc
     9241 gcgcgttgca gcgcgagccg ggcgaccgat tcccggatgc cgggtccttc gccgaggcgc
     9301 tgcgcgcctg gcagcgcgac ccggcggcgg gagccgcggc cgcagcgacg ggcgcggcgg
     9361 ccggcacttc ggcccccccg ccggctcccc tgcctcccgc cggcgagccg accatgtacg
     9421 tcccgccgcc ggtggcgcgg ccgtccgacc gggcgcccat gaacgtcaac gcgccacccc
     9481 cgcgccggcc cgcgccgcgc tacgaggaaa gccgcggcca gccgtggtgg atgtggctgc
     9541 tcctgctgct tgcggtgatc ctgctgggaa ccatcggctt cctgggtgcg cagatcctgg
     9601 gcaacctggg cccgggtgcc acgcccagcc cctccgccaa cgtggtcgag gtgcccaact
     9661 gggtcggcga gccgatcgtc gcggtacggg acgaggccga ggagctcggg ctggacctgg
     9721 acgaggacga ggagccgtcc gacgacgtcg aggagggacg cgtcatctcc acgaatcccg
     9781 ccgctggcga gcccgtcgcg gaggggggca ctgtggtcgt ggtggtcagc agcggcgccg
     9841 accaggtgca ggtgccgagc ctgatcggcc agaccgagga gcaggcggcc cagacgctgc
     9901 gcaatgccga gctggagctg gggaccgtca cccgcgagtt cagcgaggag ccggagggcc
     9961 gcgtgattcg cagcaacccc gccgccgggg tcgactggcc gcgcggcgac caggtcgaca
    10021 tcgtggtgtc ccgcggcccg gagccgacgc ccacgccatc gccgacccct gagccgaccg
    10081 tggccccaac cgctacgccc gagccgccac ctcccccgac ggcaaccccg accccgacgc
    10141 ccgccgccac gccgtagccg cccgacacat cgggcaggac ttcacgggaa agaactgcca
    10201 ggtgtcgtgc tgcggatcca tcgggcgggg ctgcgcgtga gaggcgcggg aagcctccgt
    10261 ccgtgctcac cggcgcccga cagatcggca gattgacccg tggaacctgt ttcgtgcgaa
    10321 gaatcgcccg aagcagtgcg cactgcaggt aggtggccct gcccgcccga tctatcgcgg
    10381 cagagagcgc gggaaaaggc cgattgagcg tcacctgacc gatctatgag ggcaccggac
    10441 gcgggaacac ccgatctggc cgccttcagg tgcacgatgc accgatagat gagtcagccc
    10501 gggacgcgcc gtgggcctca tcggtccaac gtgagcgttg gtgctacgat cgattctgat
    10561 catgagcacc cctcatcagg aggttccgac gtcaagcatg tctgcaccga tggcggtcga
    10621 tgaatcgacc ttcgaggcgc aggtcctcag cgctgacaag ccggtcctcg tcgacttctg
    10681 ggcaccgtgg tgcgggccgt gccggctcgt ctcacccgtg gtggagtccg tgggcgaggc
    10741 gcatgccgac aagatcggcg tggccaaggt gaacaccgac gagaaccagc agctggccat
    10801 gcgctacagc atcttcagca tccccaccct gatcctgttc aaggacggac gcgaggcggc
    10861 ccgcctggtg ggctacatgc cccaggaggc catggaggat cgcctgcgcg acttccttgc
    10921 ctgagcgtcc ttccctcgac caggaccgat ggaggccggc tcacagagcc ggccttcggc
    10981 atgcggtgcc ccgacgcacc agtgcgcgcg ggtccagccg ctacactcgt cgctgaacca
    11041 ggagggctgc ccgagcgtga tcatcaagac cgaacgtcgt tccggggccg accgccgcca
    11101 gacggaggac cccttcgccc aggagcgacg cgggcctgac cggcgcacca tgtgctggga
    11161 ggtcgagaac tgttcgctca ccattcgcta ccggtgcccg gccttcatcc tgcgccgccc
    11221 ctgctgggag ctgtgggctg tcgacggggt gggaccggca cgcgcctgct gccacaacga
    11281 gatcgactgc gagccgggcc gctgcgtcat cgccgagaag cgcttcgcga ccagtccaga
    11341 accgctgacc gtccacgtcg ggcgccgcgg cgtgccgctc ggttacgccc gaccccgccg
    11401 ccactcgtgc ccgaacttct tcctgaccca gggcgccaag ggacgggtgc ccatcgaggc
    11461 gccgcagctg ctgcgcacgc tgatgaagca ggagggcgtg gtctcccgct gccaggtccg
    11521 gcccggcgtg cacctcgacc agggctacgt caccgacctg tgcctgaccg gccagttcca
    11581 cgactgcgtc ttctacgagg acgagagcgc ctgacccctc ccgggccggt ggaccgatga
    11641 ccaccgccct ggcctacgcc gccgcggttc gcgagctccg ggagcgtggc cggttcggca
    11701 tcggcctcgg cctggagcgg gtgaccgcca tcctcgatga gctcggccat ccggagcggg
    11761 ccctccacgg cgcgctggtg gccgggacga acggcaaggg cagcgtggtg gccctcgccc
    11821 gctcggcgct cgccgccgcc tgcctgcgaa ccgggaccat gcccaagccg cacctggtca
    11881 gctaccgcga gcggatcgcg atcgatggcg tccccctgtc cgaggaccgc ttcgccgcgg
    11941 ccctggcctc cgtcctgccc gcggtcgacc gcgtcgcggc tcgcctgggt ccccccaccg
    12001 agttcgaggt cctgaccgcc gccgcctacg ccgagctggc gcgggccggg gtggacctgg
    12061 cgctggtcga ggtgggtctt ggcggacggc tggacgccac caacgtgctg gatcccggcg
    12121 tggccgtcat caccaacgtg cagcacgacc acgagcgcca cctcggtcgc acgttggcgg
    12181 ccatcggtgc cgagaaggcg gccatcatca agcgcggcaa cctggcggtc accggggcga
    12241 ccgggcgcgg cctgcgcccc atcctggacc gatgcgccgc gctggacgtc ccgctgcgcc
    12301 gggccggcag cggccagccg tacgcggtgg aggtgcgcca cgtcggctgg gacggcctgg
    12361 tggtcgatgc ccgtacgccg tcgggttgga cgcgtgacct gcacgtccgg ctgctggggc
    12421 gccaccaggc gtccaatgcg gcagtggcgc tcgccctgct cgacgcgctg cgtgacgacg
    12481 gggagcggcg cggcaaaccg ctgctcggcc tggacgaggc ggccgtgcgg gagggatttg
    12541 agcgcgtgag ctggcccggc cggatggagc tgctgccaac cacgcggctc ggccgggtgc
    12601 tgctcgacgg cgcccacaac cccgccggcg cgcgggcgct tgccgacgcg ctcgacgagc
    12661 tggggctgcg ctcgttcccg ctggtgttcg gcgccatgcg cggcaagcgg gtcagcgcgg
    12721 tgctgcgcgc cctggcgccg ctgaggccga acccggtctt cacccaggtg gagggcgggg
    12781 ccgccatccg ctcgcgcacg ctgcaggccg cctggcggcg catcacgggg agcggcgggc
    12841 agagcaccgc gccgccggcg gcggcactcg agttggctgc caccttgcgt caggatgccg
    12901 gccaacctat cgtcgtggcc ggttccctct acctcgtcgg cgacattcgt gggatgctca
    12961 ggccgcagga ggatggcgcg tgaccgagct gcgctggggc gagcggacct acgtgatggg
    13021 catcatcaac gtgacgcccg actccttctc cggcgacggg ctggcggagc ccggcgccag
    13081 cgagctggag atcgtggctg ccgcgctgca gcaggcacgc gccttcgtgg ccgccggcgc
    13141 cgacatcctg gacgtcgggg ccgaatcatc tcggccggcg caggtctacg gcgagcaccc
    13201 gccaattgac gtggaggccg agatcgcgcg ggccgtgccg gtggtcgcgg ccctctcccg
    13261 cgagttcgcc gggcgaacgc tggtcagcat cgacaccatg aagggggcgg tggcgcgcga
    13321 ggcgctggca gcaggcgccg gcatggtcaa cgacgtgtgg gccgggcgcc acgaccccgg
    13381 gacggtggac gcggcggcgg cggctggcgc atacctggtc ctgatgcaca acaaggaggc
    13441 ggccgagtac cccggcggca tgttcgggga ggtcgtggcc tggctccgct cggccgtgga
    13501 cgacgcggtc gatcgcggca tgccacgcga gcggctgctg gtggacccgg ggatcggctt
    13561 cggcaaaaag ccggcgcact cggtggaggt gctccatcgg ctggcggagc tcaaggatgc
    13621 cctcggcggg ctgccgctac tggtgggtac cagccgcaag cggttcatcg gcgagatcct
    13681 cggcggcgtg ccaccgcagg agcgcgtgga gggaaccgcg gccaccgtgg cgctggcgat
    13741 tgccgccggg gcggacgtgg tgcgcgtcca cgacgtggca cacatggtcc gcacggcgcg
    13801 ggtcagcgac gctatcgtgc ggctccgcca gtgagcgtag acgaccgcat cacgctgcgc
    13861 ggcatgcgat tcctgggccg ccacggcgtg acgctcgagg aacgcatgga accccagccg
    13921 atcgaggtcg acctgatcct gcgccgcgac ctgtcgaagg ccgttgccag cgacgagctg
    13981 gcggacacca ccgactactc agcggtgttc accctggtcg gcgccgtggt ggaagggcag
    14041 tccttccggc tgcttgaggc gctggcgggc gccatcgcgg acagcgtccg caccgagcac
    14101 gcagtggacg acgtggaggt ccgcgtgcgc aagccacagg cacccctgcc cgggacgctg
    14161 gagacggtgg aggtccgcgt gcgacgcgcc gtggcggcca gggaaaaggg ctagggctgc
    14221 tcggggagcg tccccagcgt gacggtcacc tcgcgggtgg atccgccgcc gccgccgcgc
    14281 agcacccgca gcacgatctc gtcgccgggg cggtggggca gaatgtgctc ggctaggtcg
    14341 tcctcggcgt ccacccgctg cccgtcgacc gccacgatca cgtcaccggc ctccaggccg
    14401 gcggccgcgg ccgggctgtc ggggaagacg gccggccggt tcgggttgcc ggactcgacc
    14461 agcacgccgt agtccaccgg caggtcctgg tcctccgcca gctgctgggt gaccggctgg
    14521 tagaagacac cgatccaggg ccgcgcgatc tccaccccct ccagcgcctg ggacaggatc
    14581 ggcttggcca cgtcgatcgg gatggcgaag ccgatgccct gcgcgtcctc gttgacggcg
    14641 gtgttgatgc cgatgacctg cccggcgctg tcgatgagcg gcccgcccga gttgccctgg
    14701 tt
//
LOCUS       DATACA010000493         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2236520, whole genome shotgun sequence.
ACCESSION   DATACA010000493 DATACA010000000
VERSION     DATACA010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236520"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..453
                     /locus_tag="VHK28_09985"
     CDS             91..453
                     /locus_tag="VHK28_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142579.1"
                     /translation="MSDELQTVLRLVAEGKLSPDEAAPIIEALTMRARPPAATQREPS
                     AREGRGRKVRIQVTERGRRVVDVRVPLAFAAMAAATVPGIPDSYAALIEQAVDSDNFG
                     TIVDAQDENGDGVLISVE"
     gene            459..1766
                     /locus_tag="VHK28_09990"
     CDS             459..1766
                     /locus_tag="VHK28_09990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142580.1"
                     /translation="MDSTTTSARTAPATPAASVTVAADHGPALFRPLRVRDFRLVFIG
                     ETISLMGSQFHHVAIAWLTLQLTGSGLALGAVMATAAVPRAAFILLGGALSDRLSPRT
                     LMLGSNLLRALVVAALAVLVLAGRAELWHLFVLAAIFGMVDAVFHPALGTIVPMLVSS
                     RLLPAANALTQAMRQLSQLFGPVSAGIVVATISIGAAFVFDAASFAVAAVAIMLIRGG
                     RRPRADGDGSAETLLANVRAGLAYAWSDPAVRSLLLLTAAFNFAFTGPVSVGLPYLAE
                     TRFDGGAVAFGLLVSAFGAGALAGAVVAGSLRRVPRLGLVVLVLAAGLGIGLAMVGNA
                     PTLWMALPAMGGIGLGGGFLNVRVIAWLQARTPEKMLGRVMSLVLLAGVGLAPISLAL
                     AGVVIDLGAVSLLFSVAGGVMVAAVIAGLYGGVPSRMQDGETA"
     gene            1763..>2899
                     /locus_tag="VHK28_09995"
     CDS             1763..>2899
                     /locus_tag="VHK28_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142581.1"
                     /translation="MTTLAVEAIGLVKEFERGRRTAWQRLRREPDHRERFRAVDGVDL
                     RVESGEIFGVLGPNGAGKTTTMKMLATLLEPTEGSARVLGLDVVTQARQVRRRMGAVL
                     SDGRSLYWKLTARENLEYFAALYHVPPGEQKRRISAALAAVKLDDRADDYVERFSTGM
                     RQRLVLARALLPDPELLLLDEPTVGLDPQSARDLRDRVRELQGQGRTVLLTTHYMEEA
                     DQLCDRIAIVDHGRVVALDTPGGLKRTVGANEVVHLEIADADDTRNGNHVGLLARLEH
                     VGRRQRDGPEQEAQEERRVAAQDAPLLPRGGAEGPEVAEQELEGHSRSARPVREMNTS
                     SRVTGTTSTWLMVAPSARAASNAAGTKSRALRQWIVTASTPLVLS"
BASE COUNT          403 a          988 c         1065 g          443 t
ORIGIN      
        1 ccgaagaaga gatggagatc ctccgtgccc tggagcgtgg cgagttggac gtcgaagagg
       61 cgccgcgtcg cctggccgga ggcgagccga atgagtgacg agctgcagac cgtcctgcgc
      121 ctggtggcgg agggaaagct gtcgccggat gaggcagcgc cgatcatcga ggcgctgacg
      181 atgcgcgccc gtccccctgc cgcaacacag cgtgagccgt ccgcgcgcga gggacggggc
      241 cgcaaggtgc gcatccaggt caccgagcgc ggccgacgag tcgtcgacgt gcgcgtgccg
      301 ctggcgttcg ccgccatggc ggccgccacc gtgccgggca tcccggacag ctatgccgcc
      361 ctcatcgagc aggccgtgga cagcgacaac ttcggcacca tcgtggacgc ccaggacgaa
      421 aacggcgacg gcgtcctgat cagcgtggag tagaggcgat ggattcgacc accacctccg
      481 cgcggacggc gccggccacg ccggccgcga gcgtgaccgt ggccgcggac cacggcccgg
      541 cgctgttccg gccgctgcgc gtccgcgact tccggctcgt gttcatcggc gaaacgatct
      601 cgctcatggg cagccagttc caccacgtcg ccattgcctg gctgacgctg cagctgaccg
      661 gtagcggcct ggccctgggg gctgtgatgg cgaccgcagc agtaccccgt gctgccttta
      721 tcctgctggg tggcgcgctc tccgatcgac tctcgccacg tacgctgatg ctcggctcga
      781 acctccttcg cgcgctcgtg gtggcggcgc tcgccgtgct ggtcctcgcg ggcagagcgg
      841 agctgtggca cctcttcgtg ctggccgcca tcttcggaat ggttgatgcc gtcttccatc
      901 cggcgctggg caccatcgtt ccaatgctgg tcagcagccg gctgttgccg gccgccaacg
      961 cgctgaccca ggccatgcgc cagctcagcc agctgttcgg cccggtatcc gccggaatcg
     1021 tggtcgccac catcagcatc ggcgcggcct tcgtattcga cgccgcgtcc tttgccgtcg
     1081 cggcggtcgc gatcatgctg attcgtggcg gccgccggcc cagagccgat ggtgacggct
     1141 cggccgagac gctgctggcc aacgttcgcg cggggctggc ctacgcctgg agcgacccgg
     1201 cggttcgctc gctgctgttg ctcaccgcgg ccttcaactt cgcgttcacc ggtcctgttt
     1261 cggtcggcct cccctatctc gccgaaaccc ggttcgacgg cggcgccgtc gccttcggcc
     1321 tgctggtctc cgccttcggg gcgggggcac tggccggggc ggtggtggca ggatccctgc
     1381 gccgtgtgcc gcggctgggg ctggtcgtgc tcgtgctcgc ggccggtctg ggcatcggct
     1441 tggccatggt cggcaacgcg ccaacgctgt ggatggcgct gccggcgatg ggaggcatcg
     1501 gtctgggagg tggcttcctc aacgtgcggg tgattgcctg gctccaggca cgcacgcccg
     1561 agaagatgct gggccgcgtg atgagcctcg tcctgctggc cggcgtgggc ctggcgccca
     1621 tcagcctcgc gctcgcggga gtggtcatcg acctcggggc cgtctcgctg ctgttctcgg
     1681 ttgcgggtgg cgtgatggtg gcggcggtga tcgcaggact gtacggcggg gtcccgtcgc
     1741 gcatgcagga tggggagaca gcatgaccac gctggcggtc gaggcgattg ggctggtcaa
     1801 ggagttcgag cgcggacgtc gcaccgcctg gcagcggctg cgccgcgagc cggaccaccg
     1861 cgaacggttc cgggcggtgg acggcgtgga cctgcgggtg gagtccggcg agatcttcgg
     1921 cgtgctgggc ccgaacggcg ccggcaagac cacgacaatg aagatgctgg ccaccctgct
     1981 ggagccgacg gagggctcgg cccgggtgct ggggctggac gtcgtgacgc aggcgcggca
     2041 ggtgcggcga cggatgggcg cggtcctgtc ggacgggcgc agcctgtact ggaagctgac
     2101 cgcgcgcgag aacctcgagt acttcgcggc gctctaccac gtcccacccg gagagcagaa
     2161 gcgacggatc agcgccgccc tggccgcggt caagctggac gaccgagccg acgactacgt
     2221 ggagcgcttc agcaccggca tgcgccagcg gctggtgctg gcccgcgcgc tgctccccga
     2281 ccccgagctg ctgctgctcg acgagcccac ggtggggctg gacccgcagt cggcgcgcga
     2341 cctgcgcgac cgggtgcgtg agctgcaggg ccagggccgc acggtgctgc tcaccaccca
     2401 ctacatggaa gaggcggacc agctctgcga ccggattgcc atcgtggacc acggccgggt
     2461 cgtagcgctc gacacgccgg gcggcctgaa gcgaacggtc ggcgccaacg aggtggtcca
     2521 cctcgagatc gccgacgcag acgacacccg aaacggcaac cacgtcggtc tgctggcgcg
     2581 cctggaacac gtaggccgtc ggcaacgcga tgggccagaa caggaagccc aggaagagcg
     2641 acgggtagcg gcgcaggatg cgccactcct tccgcgcggc ggcgccgaag gcccggaggt
     2701 cgccgagcag gagctggagg gtcattcgcg cagcgcccgc ccggtgaggg agatgaacac
     2761 gtcctccagg gtcaccggca ccacctcgac gtggctgatg gtggcaccct ccgcccgcgc
     2821 tgcgtcgaat gcggccggca cgaagtcgcg cgcgctccgg cagtggatcg tgaccgccag
     2881 cactccattg gtcctgtcg
//
LOCUS       DATACA010000494         6647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_468903, whole genome shotgun sequence.
ACCESSION   DATACA010000494 DATACA010000000
VERSION     DATACA010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468903"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="VHK28_10000"
     CDS             <1..2234
                     /locus_tag="VHK28_10000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX2142582.1"
                     /translation="QRFRYILVDEFQDTNYAQFELVKLLAGDPKANVTVVGDDDQSIY
                     RFRGAALSNILGFRSAYPGAASVVLTDNFRSRQPILDAAHRLIRHNDPDRLEAREGLD
                     KRLVARASFSRPVPADGPMELLAFDTVSDEADAVAERIAASMRAGRPAGDHAILVRTN
                     RDADPFLRSLNMARVPWRFSGTAGLYRQPEVRVLVSFLRAANDPEDSIACYDMATSEV
                     FGLDAGDVTRALNAATRRRISLESALRQAATREQNAPFGPRATEVVQRLLSSIDQHRG
                     MSVERTTGELLYHFMTATGWLGRLAREAREQGEERLANVARFFEIVRRQSALLRDDRL
                     PFLVAQLNTLMEAGDDPSTADVEPDEGDAVHVLTYHKAKGLEFGVVHMVGLVDDRFPT
                     RSRGDQLPLPVELVREPMPDGDHHLGEERRLFYVGMTRAREELVLSWARDYGGRAARR
                     LSQFVLEALDLPPATPVETLRPSVAQRLARHDRPASPPPAVSRPALSDKPLSLSYGQI
                     GDYLECPARYRYSHVIRIPTPASHQMAYGRALHAAVQAFHRRQLAGGSMTLDELHVEL
                     DAAWESVGFLTRQHEEARRGAARLALERFWTEQQADPARPVAVEQEFAVQLGRDRVRG
                     RYDRVDHDADGRVVITDYKTADVRDPATANRRARESLQLSIYALAHEAQHGRPPDEVA
                     LHFLDSGLLGRAVPSARRLAKATEQVVEVGEAIRGARFEATPSPMKCGFCPFREICPD
                     ALR"
     gene            2248..2655
                     /locus_tag="VHK28_10005"
     CDS             2248..2655
                     /locus_tag="VHK28_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142583.1"
                     /translation="MSGGNRLAVLVLALLSVLTVLALLLFASAACPAELPGQPCPEAG
                     TNRVVVVALAAAATGLLVTPFAFLAEFAARRRIAYRGAWGRAGRRGLLAAAGVGALAG
                     LRLGGALSVPAGIFVILLVGMVEWFAVRRFDVP"
     gene            2652..3482
                     /locus_tag="VHK28_10010"
     CDS             2652..3482
                     /locus_tag="VHK28_10010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2142584.1"
                     /translation="MSSDIFAAQPADADLLAELYDLEHDAVVEDLAFYREMARRSGGA
                     VLDAGCGSGRLFRSFVEGGAPRVVGVDGSPALLARAQLRVAADPLLRSAAGAGRLELV
                     RGDVRRLPALGLGAKLPGRGAALAVVAGVLPHLDGPEEALQLLGGVARLLAPAGRLVL
                     DDLGPGLLPQHDLPLSVDWQRAVGGRSVVRRSQLSRREAPEGLRVAFSTIVDVGQADG
                     TIARLPAAYRLWYPSHDALAALVREAGLAVELAYGSHDLDPLDEESDRRILVIRHAVG
                     "
     gene            3528..4676
                     /locus_tag="VHK28_10015"
     CDS             3528..4676
                     /locus_tag="VHK28_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2142585.1"
                     /translation="MAAEQIRLLVIEDVPQVASHMRALLQAQSQIKMLEVVTAGDRAV
                     AATSEYKPDVVIVDALLQGRVSGEQVAQGIRQAEPQIGIIMLTVPQNPVAQNAERGID
                     AVLTMPFSGFDLTTTVRKTYEERSVVASRAGSMIITLFSPKGGVGRTTIAYNLAVALA
                     ANHRVCLIDGSLQFSDLRGLLRVPATAPSVVNLPTDRIRETDVAEVVWKDPSGVDILL
                     APPRVEMAEMVTTRDMEKALSIIRQLYEFVVVDTRPALSDDVLVFLDASDLILEVLEY
                     DAMAIRALAMAGEAFDAIGYPPTKLATILNRSDSAGGFDKGDVETALGRRIDYEVVSD
                     GRLVVAANNEGVPFVSGSPDAPISHGIRRIADSLSMHLRERTPALARR"
     gene            4697..5521
                     /gene="murI"
                     /locus_tag="VHK28_10020"
     CDS             4697..5521
                     /gene="murI"
                     /locus_tag="VHK28_10020"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX2142586.1"
                     /translation="MASGSDSRPIGVFDSGVGGLTVLSELWRRLPNEATVYLGDNGRA
                     PYGPRPADEVERFTLEGVDWLLRQDIKLLVLACNTATSRALPLVRGRAPVPVLGVVRP
                     GAVTAAAATRAGHVGVVATVGTVESGAYPAAIMEANGRLSVTQQACPDLVPLVEAGRL
                     DGPETEAAVQAYLGDLLWRDRDIDTLLLGCTHYPLLRPVIERVVGPGVAVVDSAFTTA
                     IATEDLLDALALRAPANARPAHRVATTGDVAAFTGVAERLFGAALPGVEAVELAPV"
     gene            5532..>6647
                     /locus_tag="VHK28_10025"
     CDS             5532..>6647
                     /locus_tag="VHK28_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX2142587.1"
                     /translation="MTPNAGRLGAGIVIGGLLAVGATVAQREIMRRAGTRLIDWHAVR
                     AIARRRLGSDGAPLAAGLRAAAEEHYRRELLRIEPVVAEEIGGTLPRALEVPAVIDRR
                     EWVDLNLATFEVLFERVERVVNASQTGADTPGRAVARIVNRSVGNQQLGFLLAFLARK
                     VLGQYDVSLLAAAPGARGRLHFVEPNIVGTAAAMRLPLDEFRTFIALHEATHAFEFEA
                     YPWVREHFAGLVAEAIEQVAADSGGMLGRLQQALSAKGGHWLERMMTPRQLESFQRTQ
                     ALMSLLEGYSNHVMNAAGERLLPGFSEIHERFERRNEARTAVERMIMRVTGLDLKMEQ
                     YEAGERFVKAVIAARGREFLNRVWEGPAQLPSLEEIRA"
BASE COUNT          874 a         2385 c         2413 g          975 t
ORIGIN      
        1 ggcagcgctt ccgctacatc ctggtggacg agttccagga caccaactac gcccagttcg
       61 agctggtgaa gctgctggcc ggcgacccca aggccaacgt cacggtggtg ggggacgacg
      121 accagagcat ctaccgcttc cgcggcgccg ccctgtccaa catcctgggc ttccgcagcg
      181 cctaccccgg cgccgccagc gtggtgctga ccgacaactt ccgcagccgt cagcccatcc
      241 tggacgccgc ccatcggctc atccgccaca acgatccgga ccgcctggag gcacgcgagg
      301 ggctggacaa gcgcctggtg gcgcgggcca gtttctcccg cccggttcct gcagatggac
      361 cgatggagct gctcgccttc gacaccgtct ctgacgaggc ggatgccgtg gcggagcgta
      421 tcgcagcatc catgcgcgcc ggccgaccgg ccggggacca cgccatcctg gtgcgcacca
      481 accgggacgc ggatcccttc ctgcgatcgc tgaacatggc gcgcgtgcca tggcgcttct
      541 cggggacggc cggcctctac cgccagccgg aggtacgcgt gctggtcagc tttctgcgcg
      601 cggccaacga cccggaggac tcgatcgcct gttacgacat ggccacctcc gaggtgttcg
      661 gcctggatgc cggagacgtg acgcgcgccc tcaacgccgc cacgcggcgc cggatcagcc
      721 tggagtcggc cctgcgccag gcggcgacgc gagagcagaa cgcgccgttc ggcccccgag
      781 ccacggaggt cgttcagcgg ctgctctcct ccatcgacca gcaccgcggc atgagcgtgg
      841 agcgcaccac cggtgagctg ctctaccact tcatgaccgc caccggctgg ctcggcaggt
      901 tggcgcggga ggcgcgcgag cagggcgagg aacggctggc caacgtcgcg cgattcttcg
      961 agatcgttcg gcgccagtcg gcgctgctgc gcgacgaccg cctgccgttt ttggtggcgc
     1021 agctcaacac gctgatggag gctggcgacg acccctccac cgcggacgtc gagccggacg
     1081 agggtgatgc cgtccacgtc ctcacctacc acaaggccaa gggcctcgag ttcggcgtgg
     1141 tgcacatggt cggcctggtc gacgaccgat tccccacccg cagtcgcggc gaccagctgc
     1201 cgctgccggt tgagctcgtg cgggagccga tgccggacgg ggaccaccac ctcggggagg
     1261 aacgccgcct cttctacgtg ggtatgaccc gcgcccgcga ggagcttgtg ctgagctggg
     1321 cgcgcgacta cggcgggcgg gccgcgcggc ggctgagcca gttcgtgctc gaggcgctcg
     1381 atttgccgcc cgccactccg gtcgagacgc tgcggccgtc ggtcgcgcag cgactggccc
     1441 gccacgaccg gccggcgagc ccgcctccgg ccgtgagccg gccggcgctc tcggacaagc
     1501 cgctgtcgct gagctacggc cagatcgggg actacctgga gtgccccgcg cgctaccgct
     1561 actcccacgt cattcgcatc ccgaccccgg cgtcgcacca gatggcgtac gggcgcgccc
     1621 tgcacgccgc cgtgcaggcc ttccaccggc ggcagctggc cggcggcagc atgaccctag
     1681 acgagctgca cgtcgagctg gacgccgcgt gggagtcggt cggcttcctc acccgccagc
     1741 acgaggaggc gcgccggggc gcggcgcgcc tggcactgga gcgcttctgg accgagcagc
     1801 aggcagaccc cgcccgaccg gtggccgtgg agcaggagtt cgccgtgcag ctgggccgcg
     1861 atcgcgtccg ggggcgctac gaccgcgtgg accacgacgc cgacgggcgg gtggtgatca
     1921 ccgactacaa gacggcggac gtccgcgacc ccgccaccgc caaccgccgg gcgagggagt
     1981 ccctgcagct gtcgatctac gccctggcgc atgaggcgca gcacgggcgg ccgccggacg
     2041 aggtggccct tcatttcctg gactcggggc tgctgggccg cgccgtcccc tcggcgcgcc
     2101 gcctggcgaa ggcgaccgag caggtggtcg aggtcgggga ggccatccgc ggcgcccgct
     2161 tcgaggccac gccctcgccc atgaagtgcg gcttctgccc gttccgggag atctgcccgg
     2221 acgcgctgcg ctgaggttcg ggcgccggtg agcggcggca accgcctggc ggtactcgtc
     2281 ctggccctgc tgtcggtact gaccgtcctg gcgctgctgc tcttcgccag tgccgcctgc
     2341 cccgcggagc tgccgggaca gccctgcccg gaggcgggga ccaatcgcgt cgtggtggtg
     2401 gccctcgccg cggcggccac cggcctgctg gtcacgccct tcgccttcct ggccgagttc
     2461 gccgcccggc gacggatcgc gtaccgcggc gcctggggcc gcgcgggacg acgcggcctg
     2521 ctggcggccg ccggggtcgg cgccctggcc gggctgcgac tcggtggcgc gctctcggtg
     2581 ccggccggga tcttcgtcat cctgctggtc ggcatggtcg agtggttcgc cgtacgtcga
     2641 ttcgacgtgc cgtgagctct gatatcttcg ccgcccagcc cgcggacgcc gacctgctgg
     2701 ccgagctgta cgacctcgag cacgacgccg tggtggagga tctcgccttc taccgcgaga
     2761 tggcccggcg ctcgggcggg gcggtgctgg acgcgggctg cggctccggc cgcctgttcc
     2821 gctccttcgt ggagggaggc gctccccgcg tcgtcggagt ggacggctca ccggcgctcc
     2881 tcgcccgcgc gcagctgcgg gtggcggccg atccgctgct tcgctcggcg gccggcgcgg
     2941 gacggctgga gctggtccgc ggcgatgtca ggcggctgcc ggccctgggc ctgggcgcca
     3001 agcttccggg acggggcgcc gcgctggcgg tggtcgccgg ggtcctgccc catttggacg
     3061 ggccggagga ggcgctccag ctgctcggcg gcgtggcgcg gctgctcgcc cccgccggac
     3121 gcctcgtcct ggacgacctc ggtccggggc tgctcccgca gcacgatctg ccgctctcgg
     3181 tggattggca gcgtgccgtg ggcggcagat cagtcgtgcg gcgcagccag ctcagcaggc
     3241 gggaggcgcc ggagggactg cgcgtcgcct tctcgacgat cgtggacgta ggacaggccg
     3301 atggtacgat agcgcggctt ccggcagcct atcggctctg gtatccctca catgacgcgc
     3361 tcgccgcgct tgtccgggag gccggtttgg cggtggagct cgcctacgga tcgcacgacc
     3421 tcgacccact cgacgaggag agcgaccggc ggatcctggt catccgccac gccgtgggct
     3481 gacgggaagg gctcccggcc tgggcccggg agaacaagag ggcggaagtg gcagcagagc
     3541 agatccggct cctggtcatc gaggacgtgc cccaggtggc gtcgcacatg cgcgcgctcc
     3601 tgcaggcaca gtcacagatc aagatgctgg aggtggtgac cgccggcgat cgtgccgtgg
     3661 cggcgacctc ggagtacaag ccggacgtgg tcatcgttga cgcgctgctc cagggccgcg
     3721 tctccgggga gcaggtggcg cagggcatcc gccaggccga gccgcagatc ggcatcatca
     3781 tgctcaccgt gccgcagaac ccggtcgccc agaacgctga gcgcggcatt gacgcggtgc
     3841 tgaccatgcc gttctccggg ttcgacctga cgaccaccgt gcgcaagacc tacgaggagc
     3901 ggagcgtggt cgcctcgcgc gccggctcca tgatcatcac cctcttctcg ccgaagggcg
     3961 gcgtgggacg cacgaccatc gcctacaact tggcggtggc gctggccgcc aaccaccggg
     4021 tgtgcctgat cgacggatcg ctgcagttca gcgacctgcg agggctgctc cgggttccgg
     4081 ccacggcgcc atcggtcgtc aacctgccca ccgatcgcat ccgcgagacc gatgtcgccg
     4141 aggtggtctg gaaggacccc tccggcgtgg acatcctgct ggcgccgccg cgggtcgaga
     4201 tggccgagat ggtcaccacc cgcgacatgg agaaggcgct gagcattatc cgccagctct
     4261 acgagttcgt ggtggtggac acccgtccgg ctctgtcgga tgacgtgctc gtcttccttg
     4321 atgcctccga cctgatcctg gaggtgctgg agtacgacgc catggccatc cgcgcgttgg
     4381 ccatggccgg ggaggcgttc gacgccatcg gctacccgcc caccaagctg gcgaccatcc
     4441 tgaaccgctc cgactcggcc ggcggcttcg acaaggggga cgtggagacc gcgctggggc
     4501 gcaggatcga ctacgaggtc gtctccgacg gtcgactggt ggtggccgcc aacaacgagg
     4561 gcgtgccctt cgtctccggc agcccggacg cccccatttc gcacggcatc cggcgcatcg
     4621 ccgactcgct gtccatgcac ctgcgggagc gcactcccgc cctggcgcgc cgctgagcgc
     4681 cccgctccgc ggtcaggtgg ccagcgggtc cgattcgcgg cccatcggcg tcttcgactc
     4741 gggcgtcggc ggcctcaccg tcctgtccga gctgtggcgc cggctgccga acgaggccac
     4801 cgtctacctg ggtgacaacg ggcgggctcc gtacggccca cgcccggccg acgaggtgga
     4861 gcgcttcacg ctggagggcg tggactggct gctcaggcag gacatcaagc tgctggtgct
     4921 ggcctgcaac acggccacat cccgggcgct gccgctggtc cgcggaaggg cgcccgtccc
     4981 tgtgctcggc gtggtgcggc cgggagcggt caccgccgcc gccgccacac gggccgggca
     5041 cgtcggcgtg gtggccacgg tcgggacggt cgagtcgggc gcctacccgg ccgcgatcat
     5101 ggaggcgaac ggccggctgt cggtgaccca gcaggcctgc ccggacctgg tgccgcttgt
     5161 ggaggccggg cgactcgacg gccctgaaac cgaggcggcc gtccaggcgt acctcggcga
     5221 cctgctgtgg cgagaccggg acatcgacac cctgctgctg ggctgcaccc actacccgct
     5281 gctgcgcccc gtgatcgagc gggtggtcgg ccccggcgtc gcggtggtcg actccgcgtt
     5341 caccacggcc atcgccaccg aggacctgct cgacgcgctg gccctccgcg ccccggcgaa
     5401 cgcccgcccc gcgcaccgcg tggcgaccac cggcgacgtg gcggcattca ccggcgtggc
     5461 cgagcgcctg ttcggcgctg ccctccccgg ggtcgaggcg gtagagctgg cgcccgtcta
     5521 gactcagccg catgacaccc aacgccggcc ggctcggggc cgggatcgtg atcggcgggc
     5581 tgctggccgt gggcgccacc gtggcgcagc gcgagatcat gcgccgggcg ggcacgcggc
     5641 tgatcgattg gcacgccgtt cgggccatcg cccgtcgccg gctcggttcg gacggcgcgc
     5701 cgctggccgc gggcctgcgc gccgccgccg aggagcatta ccggcgcgag ctgctgcgca
     5761 tcgagccggt ggtcgccgag gagatcggtg ggacgctgcc gcgcgccctg gaggtgccgg
     5821 ccgtcatcga ccgccgcgag tgggtggacc tcaacctggc gaccttcgag gtcctcttcg
     5881 agcgcgtcga acgcgtggtg aacgcctcgc agaccggtgc cgatacgccc gggcgtgccg
     5941 tggcgcgcat cgtcaatcgg tcggtcggca atcagcagct cgggttcctg ctcgccttcc
     6001 tggcccgcaa ggtcctgggc cagtacgacg tcagcctgct ggccgccgcg cccggcgctc
     6061 gtggccggct gcacttcgtg gagccgaaca tcgtgggcac cgcggcggcc atgcgcctgc
     6121 cgctcgacga gttccgcaca ttcatcgcgc tgcacgaggc gacgcacgct ttcgagttcg
     6181 aggcgtaccc gtgggttcgc gagcacttcg ccggcctggt ggcggaggcc atcgagcagg
     6241 tggctgccga ctcgggcggc atgctgggac gcctccagca ggccctctct gccaaaggcg
     6301 gccactggtt ggaacggatg atgacgccgc gccagctgga gtcgttccag cggacccagg
     6361 cgctcatgtc gctgctcgag ggctactcca accacgtgat gaacgccgcc ggcgagcggc
     6421 tgctgcccgg cttcagcgag atccacgagc gcttcgagcg acgcaacgag gcccgcaccg
     6481 cggtggagcg catgatcatg cgggtcaccg gcctggatct gaagatggag cagtacgagg
     6541 cgggggaacg gttcgtgaag gcggtgatcg ccgcccgcgg gcgcgagttc ctgaaccggg
     6601 tctgggaggg tccggcgcag ctgcccagcc tggaggagat ccgggca
//
LOCUS       DATACA010000495         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_629919, whole genome shotgun sequence.
ACCESSION   DATACA010000495 DATACA010000000
VERSION     DATACA010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629919"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VHK28_10030"
     CDS             complement(<1..919)
                     /locus_tag="VHK28_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142588.1"
                     /translation="MRRPLVGLVVATFVVVAACEADAPSPSLAPQPPATILPDRHSDD
                     ASLIATLPNDEHSLDFANSDMAFWGDLLVHGSYDGFRVVDISDPNRPETLAEVACRGP
                     QGDISIWEHLVFVSVDRPQSSAGCESEDAQDADPLFLPDEGGWEGIRIFDVMAPSAPR
                     LVETVTADCGSHTHTLVPDVANGRVLLYVSSYALLQDERLAPECINPHGHISVVEVPL
                     DDPGAAAVITQPHLFDTPAWEVAPGAPPNWHSTAGCHDITVFIPLRLAAAACLSEGQI
                     WDLSNPERPMAIAHVDLPEVAFWHSAAWSN"
     gene            complement(916..>2142)
                     /locus_tag="VHK28_10035"
     CDS             complement(916..>2142)
                     /locus_tag="VHK28_10035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142589.1"
                     /translation="LVGATLAGREADRRGPMPPFAVGVACFAVGLVVAGLATSMEWVV
                     AGRVIQGLGSGAISAIAYVAIALGYDPVARPRMIAIISSAWVLPGLVGPALAGWITQE
                     FTWRWIFLGLAPLLPLAALATVVPLARLRREPGGEGAEPASPSAVADSVLLAVGAGVL
                     LAGLSARNLLIAAVALPVAVVLVRRPLARLLPPGTLSAREGRPAAVAVLALINVAFFG
                     VEVFVPLAVSSVRGAGTLVGGLALTAAAVTWAAGSWIQARVAVHGSRRALVVTGLSLI
                     VAGIGLATSVLVSGVPVWMAGVAWAVAGLGMGLAFSTATLSAIEGAPAGAEGSTSGAV
                     QLANILGVAVGTGVSGAVVAVSGPTIGLAPGIAIANLLMLVACAAGIAVVGRLPDTAP
                     AGAATRSMPAEHGPAL"
BASE COUNT          353 a          798 c          757 g          234 t
ORIGIN      
        1 cgttgctcca cgcagcggag tgccagaagg ccacctccgg caggtcgacg tgggcgatgg
       61 ccatcggtcg ttccgggttg gagaggtccc agatctggcc ctcgctcagg caggcggccg
      121 ccgccaggcg cagggggatg aagacggtga tgtcgtggca gccggcggtg gagtgccagt
      181 ttggcggggc gccgggggcg acctcccagg cgggggtatc gaacaggtgt ggctgggtaa
      241 tcaccgcggc ggcgccggga tcatccagtg gcacctccac cacgctgatg tggccgtgcg
      301 ggttgatgca ctccggcgcc agccgctcgt cctgcagcag cgcgtacgag ctgacgtaga
      361 gcagcacgcg gccattcgcc acatccggca cgagcgtgtg ggtgtgcgag ccgcagtcag
      421 ccgtcaccgt ctccaccagc ctcggcgccg atggtgccat gacgtcgaag atgcgaatgc
      481 cctcccagcc gccctcgtcc ggcaggaaca gcgggtcggc gtcctgcgcg tcctccgact
      541 cgcaccccgc gctgctctgc ggccggtcga cgctcacgaa gacgaggtgc tcccagatgg
      601 agatgtcgcc ctgcggcccc cgacacgcaa cctcggccag ggtctcggga cggttcggat
      661 cgctgatgtc caccacccga aacccgtcat agctgccgtg caccagcagg tctccccaga
      721 aggccatgtc cgagttggca aagtccaggg aatgctcgtc gttcggaagc gtggcgatca
      781 ggctcgcgtc gtccgagtgc cggtccggga ggatcgtcgc cggcggctgg ggggcgaggg
      841 acggcgaagg cgcatccgcc tcgcacgcgg caacgacgac gaacgtcgcc accaccagcc
      901 cgaccagcgg cctcctcaca gcgccgggcc gtgctcggcg ggcatggacc gagtggccgc
      961 cccggccggc gccgtgtccg ggaggcgccc gaccacagcg atcccggccg cgcaggcgac
     1021 cagcatcagc aggttggcga tggcgatgcc cggcgccagg ccgatggtcg gccccgagac
     1081 cgccaccacc gccccggata ctccggtccc caccgccacg cccaagatgt tcgccagctg
     1141 gacggcgccc gaggtggacc cttccgcgcc ggccggcgcc ccctcgatgg ctgacagggt
     1201 ggcggtcgag aaggcgaggc ccatccccag gccggcaacc gcccaggcca ctccggccat
     1261 ccagaccggc acgccggaca ccaggaccga tgtcgccagc ccgatgccgg ccacgatcaa
     1321 agacaaaccg gtgacgacga gcgcccgccg agagccgtgc accgcgaccc gtgcctggat
     1381 ccaggagccg gcggcccatg tgaccgccgc ggccgtcagc gccaggccgc cgaccagcgt
     1441 cccggccccg cgcaccgagg agacggccag cgggacgaag acctccacgc cgaagaaggc
     1501 gacgttgatg agcgccagca ccgccacggc cgcgggccgt ccctctcggg cggagagcgt
     1561 cccgggaggc agcaggcgcg ctagcggtcg ccggacgagc accacggcga ccggcagggc
     1621 gacggcggcg atcagcaggt tccgcgcgga gaggcccgcc agcaggacgc ccgcaccgac
     1681 cgccagcagc accgaatccg caacggcgga gggcgacgcc ggctctgcgc cttcaccgcc
     1741 tggctcccgg cgcaggcggg ccagcgggac gacggtggcc aatgcggcca gcggcaggag
     1801 gggcgcaagg ccgaggaaga tccagcgcca ggtgaactcc tgcgtgatcc atcccgccag
     1861 tgccgggccc accagcccgg gcagcaccca ggcgctggag atgatggcga tcatccgtgg
     1921 ccgggccacc gggtcgtacc cgagcgcgat cgccacgtag gcgattgccg agatcgcgcc
     1981 ggagcccagg ccctggatca cccggccggc gaccacccac tccatggatg ttgcaaggcc
     2041 ggccaccacc aggcccaccg caaagcaggc gacaccgacc gcgaagggag gcatcggtcc
     2101 acgccggtcc gcctcgcgtc ccgccagcgt ggcacccacc ag
//
LOCUS       DATACA010000496         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2156290, whole genome shotgun sequence.
ACCESSION   DATACA010000496 DATACA010000000
VERSION     DATACA010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156290"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VHK28_10040"
     CDS             complement(<1..522)
                     /locus_tag="VHK28_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2142590.1"
                     /translation="MTQPTFLDLPSAPPNHAPEGLNVAILGIPHGVAYPDPGPTAGCA
                     EAPAAIRARSARLAQFAAHHDFDLDGPMLPPGSAVRLFDAGDVEGSAEDGPHNQQRAE
                     AAVAELLESGVVPIALGGDDSIPIPVLRAYGGRGPLWVLHVDAHLDFRHEVHGEREGY
                     SSPMRRAAELPEVA"
     gene            complement(519..971)
                     /locus_tag="VHK28_10045"
     CDS             complement(519..971)
                     /locus_tag="VHK28_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX2142591.1"
                     /translation="MTAAPSAPQWSAVRDRFRQRGLRWTPQRRTVVEVIAAVDGHITG
                     AELVERCRARDPQTIPSTVYRTLDVLEQLGLVRHGHGPDGQEEYHVGPDAEHGHLYCA
                     ECGGHWEIASTEADGIVNAFRDSTGFEVDVSHVTVVGRCASCSVGARA"
     gene            1045..1755
                     /locus_tag="VHK28_10050"
     CDS             1045..1755
                     /locus_tag="VHK28_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015783308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEX2142592.1"
                     /translation="MPAAISIPLSDALRSWELHTPDGFLTLPVSLAMWAVTAVVLVLA
                     VRFTNRAIDERMVPLLGVTAAFIFAAQMVNFPVYPGTSGHLLGGVLAAVLLGPWAGTV
                     VMACVVGVQALLFTDGGLVVMGANIFNMGIVGTLGGYAIYRSLARALGGEERGRLPAA
                     AVAAWIAVMGGALGIALQLGLSGATDLAVALPVILGLHALIGLGEAAITVAALAFIGN
                     VRGELLQLRHASGSGPQA"
     gene            1752..2066
                     /locus_tag="VHK28_10055"
     CDS             1752..2066
                     /locus_tag="VHK28_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HEX2142593.1"
                     /translation="MREQGPRRGLPRWWWLAGLAVAAAIVVILAPLASSDPDGLERVA
                     IDQGFDDRASETPFSLMADYLFPGVEGPLATILAGLIGVAVVFAVVWLLGRLLARRER
                     QR"
     gene            2107..2868
                     /gene="cbiQ"
                     /locus_tag="VHK28_10060"
     CDS             2107..2868
                     /gene="cbiQ"
                     /locus_tag="VHK28_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HEX2142594.1"
                     /translation="MHAADARLKFVLTVAAIVAVALVPRGAFAALGIGLLGLTVASAS
                     ANLGPFRLARGAVIALPFLLATVPLVFLGEGPVLATWNLGPLSLTVWEEGLRSFATIA
                     AKSWLSVQAALLLTYTTPFHELVDALRKLRLPEVMVAIIGFMVRYLDVLADEAGRLMR
                     ARASRSAAATGRRPGGTLGWRARVTGGLVGSLFLRSYERSERIYAAMQARGFSGTFRH
                     FHGRTVTSTEWVVAAALAMLLVAFAAAANFWLPRL"
     gene            2880..>3370
                     /locus_tag="VHK28_10065"
     CDS             2880..>3370
                     /locus_tag="VHK28_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX2142595.1"
                     /translation="MPPTNADPATAHVHPHRHDGDDGHRHDHQPETSGVELPADRALE
                     LEHVHYVYPDGTEALRGVDLVLGRGEKVGLIGPNGAGKSTLMLHLNGILLPTHGSVRV
                     TGMVVDRRTVSQVRAAVGLVFQDPDDQLFSPTVFEDVAFGPLHMGVPEAEIHARVEAA
                     LAAV"
BASE COUNT          435 a         1209 c         1211 g          515 t
ORIGIN      
        1 cgccacctcc ggcagctcgg cggcgcggcg catgggcgac gagtaaccct ctcgctcgcc
       61 gtgcacctcg tggcggaagt ccaggtgcgc gtcgacgtgc agcacccaca gcggcccgcg
      121 gccgccgtag gcacgcagca ccggaatcgg gatcgaatcg tcgccgccca gcgcgatggg
      181 taccaccccc gactccagga gctccgcgac cgccgcttcc gcccgctgct ggttgtgcgg
      241 gccgtcttcg gcgctgccct cgacgtcccc ggcatcgaac aggcgcacgg cggagccggg
      301 cggcagcatc ggtccatcaa ggtcgaagtc gtggtgggcg gcgaactgcg caaggcgggc
      361 cgaccgggcg cggatggcag ccggggcctc cgcgcacccc gcggtgggac ccggatccgg
      421 gtaagcgacg ccgtggggaa tgcccaggat ggcgacgttg aggccctcgg gcgcgtggtt
      481 cggcggggcc gagggcaggt ccaggaaggt cggctgcgtc acgcgcgggc tccgaccgag
      541 cacgaggcgc agcggccgac caccgtcacg tgggagacgt ccacctcgaa cccggtggag
      601 tcgcggaagg cgttgacgat gccgtccgcc tcggtgctgg cgatctccca gtgcccgccg
      661 cactcggcgc agtacaggtg cccgtgctcc gcatccggcc cgacgtggta ctcctcctgg
      721 ccgtccgggc catgaccgtg gcgcaccagc ccgagctgct ccaggacgtc gagggtgcgg
      781 tacacggtgg aaggaatggt ctgtgggtca cgcgcccggc agcgctcgac cagctcggcc
      841 ccggtgatgt ggccgtccac cgccgcgatc acctccacca ccgtccggcg ctgcggcgtc
      901 cagcgcagcc cgcgctggcg gaagcggtca cggacggcgg accattgcgg ggcagatggc
      961 gcggcggtca tgcggccgag tatacggaac atctcgctct tgcaacatct tgcaacaaga
     1021 acggcacggg cctagaatcg ccgcatgccg gccgccatct ccattccgct ctccgacgcg
     1081 ctccgttcct gggagctgca cacgcccgac gggttcctga cgcttcccgt ctcgctggcc
     1141 atgtgggccg tcaccgccgt ggtgctggtc ctggcggtgc gctttaccaa ccgcgccatc
     1201 gacgagcgaa tggtgccgct gctgggcgtg accgccgcct tcatcttcgc cgcccagatg
     1261 gtcaacttcc cggtctaccc gggcaccagc ggccaccttc tgggtggcgt gctggccgcc
     1321 gtgctcctcg gcccgtgggc cggcaccgtc gtcatggcct gcgtggtcgg ggtccaggcg
     1381 ctgctcttca ccgacggcgg gctggtggtg atgggcgcga acatcttcaa catgggcatc
     1441 gtcggcacac tgggcgggta cgccatctat cggtccctgg ccagggccct gggcggcgag
     1501 gagcggggcc ggctgccggc ggcggccgtc gcagcctgga tcgcggtgat gggcggcgcg
     1561 ctcggcatcg ccctgcagct cgggctctcg ggcgccacgg acctggcggt ggcgctgccg
     1621 gtcatcctgg ggctgcacgc gctgatcggc ctcggggagg ctgccatcac ggtggcggcg
     1681 ctggccttca tcggcaatgt gcggggcgag ctgctccagc tgcgccacgc ctccgggtca
     1741 ggaccgcagg catgagggag cagggtccgc gccgcggcct tccgcgctgg tggtggctgg
     1801 ccgggctggc cgtggcggcg gcgatcgtgg tgatcctggc accgctggcc tcctcagacc
     1861 cggatggcct cgagcgggtg gcaatcgacc agggcttcga cgatcgggcc tcggaaacgc
     1921 cgttcagcct gatggccgac tacctcttcc ccggcgtcga gggtccgctc gccaccatcc
     1981 tggccggact gatcggggtg gcggtcgtgt tcgcggtcgt ctggctgctc ggccgcctgc
     2041 tcgcccgacg cgaaaggcag cgctgacgag atcgagctcg accgcgagat ccctcgcgac
     2101 tcgcccatcc acgcggccga cgcgcggctg aagttcgtcc tgacggtggc agccatcgtg
     2161 gccgtagcgc tggttccacg cggggcgttc gcggcgctgg gcatcggcct gctggggctg
     2221 acggtggcct ccgcatcggc caacctgggt cccttccgtc ttgctcgcgg agcggtgatc
     2281 gcgctcccct tcctgctggc cacggtcccg ctcgtcttcc tcggagaggg cccggtgctg
     2341 gcaacctgga acctcgggcc actgtcgctc acggtgtggg aggaggggct gcgctcgttc
     2401 gccaccatcg ccgccaagag ctggctctcg gtccaggctg cactgctcct gacctacacc
     2461 acgccgttcc acgagctggt ggacgcgctc cgcaagctgc ggctgccgga ggtgatggtg
     2521 gccatcatcg gcttcatggt ccgctacctg gacgtcctgg ccgatgaggc cggccgcctg
     2581 atgcgagccc gcgccagccg gtccgcggca gcaaccgggc gacgaccggg cgggacgctg
     2641 ggctggcgcg cacgggtgac cggtgggctg gtcgggtcgc tcttcctgcg cagctacgaa
     2701 cgatcggagc gcatctacgc ggccatgcag gcccgcggct tctccggcac cttccgccac
     2761 ttccacggcc ggacggtgac ctccaccgag tgggtggtgg ctgccgcact ggccatgctg
     2821 ctggtcgcct ttgccgcagc cgccaacttc tggctccccc ggctgtagga gccagaccga
     2881 tgcctcccac caacgccgac cccgccaccg cccacgtgca tccgcatcgc cacgacggcg
     2941 atgacgggca ccgccatgac caccagccgg agacgagcgg cgtcgagctg cccgccgacc
     3001 gcgccctgga gctcgagcac gttcactacg tgtacccgga cggaaccgag gcgctgcgcg
     3061 gggtggacct ggtcctgggg cgcggcgaga aggtggggct catcggtcca aacggcgccg
     3121 gcaagagcac gctgatgctg catctcaacg gcatcctgct gccgactcac ggcagcgtgc
     3181 gggtgacggg catggtggtg gaccgcagaa ccgtcagcca ggtgcgggcc gcggtcgggc
     3241 tggtcttcca ggaccccgat gaccagctct tcagccccac cgtcttcgag gacgtggcgt
     3301 tcggcccgct gcacatgggc gtgccggagg ccgagatcca cgcccgggtg gaagcggccc
     3361 tggccgcggt
//
LOCUS       DATACA010000497         3287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1754576, whole genome shotgun sequence.
ACCESSION   DATACA010000497 DATACA010000000
VERSION     DATACA010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1754576"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VHK28_10070"
     CDS             complement(<1..437)
                     /locus_tag="VHK28_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2142596.1"
                     /translation="MKVSFVAGFGPIVRDVGASHEFWGGALGIGLEEAAPQYWTNDDL
                     EGVKAFALWPLAQAAESTFGTDAWPDDIPAPQAWLELDVESPEAVGTAVEELRAAGHR
                     VLRDAHEEPWGQTTCRVLTPEGLLLGVTYTPWMHKGAGEEPGP"
     gene            complement(434..1441)
                     /locus_tag="VHK28_10075"
     CDS             complement(434..1441)
                     /locus_tag="VHK28_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX2142597.1"
                     /translation="MDFGFTLKPDHRFERAVYLARQAEANGFSHGWLFDSHVLWREPY
                     PLLTLMGTNTERMRLGTCVTNPGTRDITVTASALATLNEITGGRMDLGMGRGDSARRV
                     MGKKPLTVAYMEEACRDIRALTAGEAVTIDGTKVQLTWAEHGPVPVWIAGYGPMALAA
                     AGRVADGVILQLADPDIIRWCLGFMRAAAEEAGRDPDAIQVMAAAPVWVSDDLGAARD
                     RVRWFPALVSNHVVDLINRYPKDDLPEALWRYVEHREGYDYQHHAEVGSDNARFVSDE
                     ITDRFCIVGPPSVQRERLEELRDAGVTQFNIYLMNGDEEDCLDVYGKRVIPGLAGVGA
                     G"
     gene            complement(2121..2582)
                     /locus_tag="VHK28_10080"
     CDS             complement(2121..2582)
                     /locus_tag="VHK28_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142598.1"
                     /translation="MRRIALALALVIGSMLLPAGSHGHVTMQWGWATELVSRYDSPSC
                     LSSGAVWHQELGSSGITRLRANFERRGANDPGIPGLAYGSTGWMYTIPFPDDYRNFYA
                     TWFPAFRYPPGGVYHVRAILIGERPSFWQPDRKITTSLGEIACIVEVEAFS"
     gene            complement(2579..>3287)
                     /locus_tag="VHK28_10085"
     CDS             complement(2579..>3287)
                     /locus_tag="VHK28_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142599.1"
                     /translation="PLFMLALLAAACSTTGDGVLEVSRSPELSATGIGEPTGGSPPAG
                     WVWDAAPAEDHTHVTGDVEAEAGDPADQPDSEALGDVAIEPPSAGPLVPDPVNGEGVL
                     DPCALVLIPEWEAWTDEALGAEGLGTPATVLEDGEACGWMAAGDRVRMALGVFRAGGT
                     GRWLSDAEVAAATPIDGLGHDAFWLSAWPVEQSSTLVVQAADLDVVIEMSARDAPDAL
                     LRDGARHFATLVLDRLP"
BASE COUNT          539 a         1177 c         1058 g          513 t
ORIGIN      
        1 atcggcccag gctcctcacc ggcgcccttg tgcatccacg gcgtataggt gacgcccagc
       61 agtaacccct cgggtgtaag cacgcggcag gtggtctggc cccagggctc ctcgtgcgca
      121 tcgcgcagga cgcgatgtcc cgcggccctc agctcctcca ccgcggtgcc cacggcctca
      181 ggtgactcca cgtccagctc cagccaggcc tgcggggcag ggatgtcgtc cggccaggca
      241 tcggtcccaa aggtggactc ggcggcctgc gcgagcggcc agagcgcgaa ggccttgacg
      301 ccctcgaggt cgtcgttggt ccagtactga ggagctgcct cctccagacc gatgcccaac
      361 gccccgcccc agaactcatg tgaagccccg acgtcgcgaa cgatggggcc aaaacctgcc
      421 acgaagctga ccttcatccc gcgccgaccc ccgccagccc cggaatcacc cgcttgccgt
      481 acacgtccag gcaatcctcc tcgtcgccgt tcatcaggta aatgttgaac tgcgtcaccc
      541 cggcgtcgcg cagctcctcg aggcgctcgc gctggaccga cggcgggccg acgatacaga
      601 accggtcggt gatctcgtcg ctcacgaagc gtgcgttgtc cgagccgacc tcggcgtggt
      661 gctggtagtc gtagccctca cgatgctcga cgtagcgcca cagcgcctcg gggaggtcgt
      721 ccttcgggta gcggttgatg aggtcaacga cgtggttgct gaccagcgcc ggaaaccagc
      781 gcacgcggtc gcgcgcggcg cccaggtcgt cgctgaccca gaccggcgcg gcggccatta
      841 cctggatggc gtccgggtcg cgcccggcct cctcggccgc ggcccgcatg aagccgaggc
      901 accagcggat gatgtccggg tcggccagct gcaggatcac cccgtcagcc acccggccgg
      961 ccgccgccaa cgccatgggc ccgtacccgg cgatccaaac cggcaccggc ccgtgctcgg
     1021 cccacgtgag ctgtaccttg gtgccgtcga tggtcaccgc ctcgcccgcc gtcagcgccc
     1081 tgatgtcgcg gcaggcctcc tccatgtagg ccacggtcag cggcttcttg cccatcaccc
     1141 gccgcgcgct gtccccgcga cccatgccca ggtccatgcg gccgccggtg atctcgttga
     1201 gggtcgccag cgccgacgcg gtcaccgtga tgtcgcgcgt cccggggttg gtgacgcagg
     1261 tgccgagccg catgcgctcg gtgttggtgc ccatcagggt cagcagcggg tacggctcgc
     1321 gccagagcac gtggctgtcg aacagccacc cgtggctgaa gccgttggcc tccgcctggc
     1381 gggccaggta caccgcgcgc tcgaaccgat ggtcaggctt cagcgtgaac ccgaaatcca
     1441 ttcgccagtt cgcctccgtt cgtcatgccc gatgaagaag gcacggtaca ccggcgcgca
     1501 aggcgggtgg ggcgaggcgt tccaccgccc tgcgcgggcg aaccgtccga tttcacgtcg
     1561 ggcgtgcacc cgatgagaaa ttcgggcggc gagaggtccg aatccactag tggcgtgaat
     1621 ccgacccctt aaaccgcgct tcaccgcacg tattggtcat gtggcgtgaa gacggaccgt
     1681 tcaagcctac ccgccgcctt ggatggcttc ggccaccgtg ccagcggcca cgaaggcatc
     1741 agccaacatc tcggcacgca tctcgtcggt catggtcccg ccgttgttct ttcgccacat
     1801 tcagacgaag tgcgcgccga cgtccaaatc agagtccgcc accgccggat gccggcctgg
     1861 acatgcaggg tggcagtgcg gcaagcagtt tcgagcgtcc gtgcggggct cgcctgaatc
     1921 tggcgacttc agtgcgccct gcgcgcccgg cgaacggtca ggattcacgc cagggccgca
     1981 ttcaccgcgg aatcgagctc ccgaagggtt tggattcaca cctggcgtga attcagacgc
     2041 ttgaacgctg cttcacagcg cgtattggtc atgtggcgtg aattccgacg gttcgagctc
     2101 ttgtcttcgg cacagaagct tcaggagaac gcctccacct cgacgatgca ggcgatctca
     2161 cccaggctgg tggtgatctt acgatccggc tgccagaagc tcgggcgctc tccgatgaga
     2221 attgcgcgaa cgtggtacac cccgccgggc ggatagcgga acgccggaaa ccaggtggca
     2281 tagaagtttc ggtagtcgtc gggaaatggg atcgtgtaca tccaaccggt cgaaccgtag
     2341 gcgagtccgg gtatcccagg atcgttcgcg ccgcggcgct cgaagttcgc ccgcaggcgg
     2401 gtgatgcccg aagaccccag ctcctggtgc cacaccgcgc ccgagctcag gcacgacggc
     2461 gagtcgtagc ggctcaccag ctcggtcgcc catccccact gcatggtgac gtggccgtgg
     2521 ctgccggcgg gaagcagcat ggagccgatc accagtgcca gggcgagtgc gatgcgcctc
     2581 atggcaggcg gtccaggacc aacgtggcga aatgccgcgc gccatcccgc aggagggcat
     2641 cgggcgcatc gcgcgcggac atctcgatca cgacatcgag gtcagccgcc tgtacgacaa
     2701 gggtcgaaga ctgctccacc ggccacgcgc tcagccagaa cgcgtcatgg cccagtccat
     2761 cgataggcgt ggcagccgcc acctcggcat cgctcagcca gcggccggtg ccaccggcac
     2821 ggaagacccc cagggccatt cgtacgcggt cgccagcagc catccagcca cacgcctcac
     2881 catcctcgag gactgtggcc ggggttccga gcccctccgc cccgagcgct tcatcggtcc
     2941 aggcttccca ttcggggatc agcaccaggg cgcacgggtc cagcaccccc tcgccgttca
     3001 ccgggtcggg cacaagcggc ccggcggatg gcggctcgat ggcgacatcg cccagcgcct
     3061 ccgaatccgg ctgatcagcc ggatcaccgg cctccgcctc cacgtcccct gtcacgtggg
     3121 tgtggtcctc ggcaggcgcg gcatcccaca cccacccggc gggcggcgat ccgccggttg
     3181 gctcgccgat cccggttgcg ctcaattcgg gcgagcggga aacctccaat accccatcgc
     3241 cggtcgtgct gcacgccgcg gcgagcagcg ccagcatgaa gagcggt
//
LOCUS       DATACA010000498         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2077133, whole genome shotgun sequence.
ACCESSION   DATACA010000498 DATACA010000000
VERSION     DATACA010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077133"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VHK28_10090"
     CDS             complement(<1..503)
                     /locus_tag="VHK28_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX2142600.1"
                     /translation="MKRFRPFVALATTVLIVGACTGPGASPDESESEAASQSEAASPS
                     EAAEVPDELVIGSVPSREAGQLIEDIQPIAEFLSEELGIPVRGEVTTDYTALVTAMEA
                     GQAHIGFLPPYGMVQAVDRSNAEIILQSARFGSTTYHTQFFTNEPDKYCSDEPVENTR
                     MADDQELT"
     gene            complement(653..793)
                     /locus_tag="VHK28_10095"
     CDS             complement(653..793)
                     /locus_tag="VHK28_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142601.1"
                     /translation="MSGRRGRRGRPYQPLPQRHVNWGARLLIVFVAFVMVAGFAILTF
                     AR"
     gene            complement(790..1251)
                     /locus_tag="VHK28_10100"
     CDS             complement(790..1251)
                     /locus_tag="VHK28_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142602.1"
                     /translation="MMVHLYDYTVPAADAAESIPNLRGALADLPDEDRKALLGGLFKP
                     ERMPGTMERLTTLVADLDASFADLLIVLDNSARARKFVAIDEAKAILDRYLPESAVAG
                     THDWDVARLHEPYGDGTYHGKLLKRVRKQLRSVSSREALTTPFLLRREWGR"
BASE COUNT          252 a          576 c          544 g          260 t
ORIGIN      
        1 gtgagctcct ggtcatcggc catccgcgtg ttctcgactg gctcgtccga gcagtacttg
       61 tccggctcgt tggtgaagaa ctgggtgtgg taagtcgtgc tcccgaatcg cgcggactgc
      121 aggattatct ccgcgttcga ccggtccacc gcctgaacca tgccgtacgg cggcaggaag
      181 ccgatgtgcg cctggcctgc ctccatggcg gtcaccaggg cggtgtagtc ggtggtcacc
      241 tcgccccgca ctggaatgcc cagctcctcc gacaggaact cggcgattgg ctgaatgtct
      301 tcaatcagct ggccggcctc gcgcgacggg acggagccga tgaccagctc gtcgggaacc
      361 tccgccgcct cggacgggct ggccgcctcc gactgcgatg cggcctcgga ctccgactcg
      421 tccggtgagg cacctggacc cgtgcaggcc ccgacgatca ggaccgtcgt cgcgagcgcg
      481 acgaatggac gaaagcgctt catctgtcct ccaaaggcgc cgtggtgggc tcggcgctcg
      541 agctcggtcc gaccgccaac gggccgtccc ctcaacggcg gcatgatagc ggcagagggc
      601 aactggggta ctcgccggcg gttgcgccag cgcccagccc ggcagcggcc ccttagcgag
      661 cgaaggtcag gatcgcgaac ccggcgacca tcacgaaggc gacgaacacg atcagcagcc
      721 gcgcgcccca gttgacgtgg cgctgcggca gcggctggta gggccgtccg cggcgtccgc
      781 gacgaccgct catcggcccc actccctgcg caggaggaac ggggtggtca gcgcctcgcg
      841 gctggacacg ctgcgcagct gcttgcgcac ccgcttcagc agcttgccgt ggtacgtgcc
      901 gtcgccatag ggctcgtgga ggcgcgccac gtcccagtcg tgcgtgccgg ccaccgctga
      961 ctccgggagg tatcggtcca ggatggcctt ggcctcgtcg atggccacaa acttccgggc
     1021 tcgggcggag ttgtcaagca cgatgagcag gtcggcgaag ctggcgtcga ggtcggccac
     1081 cagcgtcgtc agccgctcca tggtgccggg catgcgctcc ggcttgaaga gaccgcccag
     1141 gagggccttg cgatcctcgt ccggcaggtc ggccagcgcg ccgcgcaggt tcgggatgct
     1201 ctccgccgcg tccgcggccg gcactgtgta gtcgtacagg tggaccatca tgggtccctt
     1261 caatgtagac cgatacgtgg gcggccgctg cggcgtcgct ccgcagcgcg aaccgtcccg
     1321 attcacgcca ggtgcgcgtt atacgccgcc agccccgccc gaaccgtcca agttgacgcc
     1381 cgagtgcgca tttctaccct ccgcgcaccc ttcggggcgc atcacgcgca cctcatgtga
     1441 attcgcgcac tcgcggccac gttgcaggcg tggcttcgcc agttgcagac cccgcgcgca
     1501 cctcccatcc cgctccatgg cccaacggac gcgcgcgtct acaatcggtg cgcgccgaag
     1561 aggccatcgg tccaacccac aggaggtcca cacgcacgtg ctgctggaag gcaagaagct
     1621 gctgatcacc gg
//
LOCUS       DATACA010000499         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2237221, whole genome shotgun sequence.
ACCESSION   DATACA010000499 DATACA010000000
VERSION     DATACA010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2237221"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VHK28_10105"
     CDS             <1..835
                     /locus_tag="VHK28_10105"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2142603.1"
                     /translation="EAIAQAAGLGLSTGTPIPGTGTLTAGWAKAYMDGALGSRTAALF
                     EPYSCGGAADRGLARLSLEEVDELLRAGRMHHVALAVHVIGDRAAATVLDAMERAPRP
                     RAGTPPDRLEHLQLLRAADRARLAAVDVTASVQPAHCAADRAMVEECWAGRQALAYPY
                     RSLADAGARLAFGSDAPIETHNPWVGIFAAVHRRFPADGSADWQPQQALDAVSALAAY
                     TSGPAMAAARTDTGHLRPGARADLAVLNVDRASMLSAGEELADARSLLTLVDGREVHR
                     S"
     gene            921..1184
                     /locus_tag="VHK28_10110"
     CDS             921..1184
                     /locus_tag="VHK28_10110"
                     /inference="COORDINATES: protein motif:HMM:NF042901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142604.1"
                     /translation="MTDVRDEPGAFHVDEPTHRPVGPGPADSTFDPAVLDFGHYAGRS
                     IEELSSIDPDYLRWLERHPSGVRYRAEIRRVLGVTPSSIDWSR"
     gene            1261..2262
                     /locus_tag="VHK28_10115"
     CDS             1261..2262
                     /locus_tag="VHK28_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142605.1"
                     /translation="MSSPPPDPGGIIAMSGVTRRFNGMTAVHDLSLEVPQGHILGLIG
                     PSGSGKTTTIRMLTGTLRPSSGRIRVLEQDPTRFTRPVRQQIAYMPQLFSLYPDLSAR
                     ENVGFVAALYGYSWFGREARIRHALQVVDLWDVRNRLARDLSGGMQRRLELACAIVHD
                     PRVLFIDEPTAGIDPMLRRAIWDELRGMRDDGRTLLVTTQYVGEAEYCDEVALLAEGE
                     LVALDAPEALRRHVYGGDVLEIDTDRAVDPEVVAPVREVMEVRQPAPRRLIVVVPDAG
                     TATPRLMDALQQRGISVVAIEEYQPTFDEVFTDLVNRRRREREEEANGAAPGGNHAA"
     gene            2252..>2707
                     /locus_tag="VHK28_10120"
     CDS             2252..>2707
                     /locus_tag="VHK28_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142606.1"
                     /translation="MLRSLWRSLVSITSFLGKELREVIRRPGVLASLILGPFLIMALF
                     GIGYTGNRSPFKTEIVVPENSQLSRDPEFYADLAPGRLDVVGVTADRAAAEERLRAKE
                     TDLLVVAPEDAAAQLRAGEQTEIGVAWNEIDPVYDSLAHLAVATMVSELN"
BASE COUNT          385 a          964 c          965 g          393 t
ORIGIN      
        1 cgaggccatc gcccaggcag ccggcctggg gctgtccacc ggcacgccga ttcccggtac
       61 cggcacgctg accgcgggct gggcgaaggc ctacatggat ggcgccctcg gatcgcgtac
      121 cgcggcgttg ttcgagccgt actcctgcgg cggcgccgcg gaccgcggcc tggcccggct
      181 gagcctcgag gaggtcgacg agctcctgcg ggccgggcgg atgcaccacg tggcactggc
      241 cgtccacgtc atcggcgacc gggcggcggc cacggtgctg gacgccatgg agcgggcgcc
      301 gcggcctcgc gccggcacgc cgccggaccg cctcgagcac cttcagctgc tgcgcgccgc
      361 ggaccgggca cgtctcgccg ccgtcgacgt gacggccagc gtgcagccgg cgcactgtgc
      421 cgccgaccgg gccatggtcg aggagtgctg ggccggccgc caggcattgg cctacccgta
      481 ccgttcgctg gcagatgccg gcgcgcgcct ggcgttcggg tcggatgctc cgatcgagac
      541 ccacaacccg tgggtcggga tcttcgcggc ggtccaccgc cggttcccgg cggacggcag
      601 cgccgactgg cagccgcagc aggcactgga tgcggtgtcg gccctagccg cgtacacctc
      661 gggtcccgcc atggcggcag cacggaccga tacgggtcat ctccggccgg gcgctcgggc
      721 cgacctggcc gtgctgaacg tcgaccgggc cagcatgctg tccgcggggg aggagctggc
      781 cgacgcgcgg agcctcctga ccttggtgga cgggcgggag gtgcatcggt cctgaccgcg
      841 cgccatttcg ttgcgcgcgc gcaccacgtc ggcgtacgat gcctgccagg cgagcaaccg
      901 aagcctgacg gaggattccc gtgaccgacg ttcgcgacga gccgggggca ttccacgttg
      961 acgagccgac ccatcggccg gttggcccag gcccggccga cagcacgttc gaccccgctg
     1021 tgctcgactt cgggcactac gccggacggt ccatcgagga gctctcgagc atcgacccgg
     1081 actacctgcg ctggctggag cgccaccctt ccggggtccg ctaccgcgcc gagatccgcc
     1141 gagtcctggg ggtgacgccc agctccatcg actggagccg ctgacccccg ctcgctcacc
     1201 cggcgggata ggggtcgctc gccggagagg cgggcacgcg gcttgcatcg gggcggcgtc
     1261 gtgagcagtc caccgcccga ccccggaggc atcatcgcca tgtccggcgt cacccgtcgc
     1321 ttcaacggga tgaccgccgt ccacgacctg tcgctggagg tgccgcaggg ccacatcctg
     1381 ggtctcatcg gcccaagcgg atccgggaag acgaccacca tccgcatgct gaccggcacg
     1441 ctgcggccgt cgagtggacg catccgggtc ctggagcagg acccgacgcg cttcacgcgc
     1501 ccggtacgcc aacagatcgc ctacatgccg cagctcttct cgctgtaccc ggacctcagc
     1561 gccagggaga acgtcggttt cgttgcggcg ctgtacggct attcctggtt cgggcgcgag
     1621 gcgcgcatcc gtcacgcgct gcaggtggtc gacctctggg acgtccgcaa ccgccttgcg
     1681 cgggatctct cgggtggcat gcagcgccgc ctcgagctcg cctgcgccat cgtccacgac
     1741 ccgcgcgtgc tgttcatcga cgagcccacc gcgggcatcg accccatgct gcggcgcgct
     1801 atctgggacg agctgcgggg catgcgcgac gatggccgca cgctgctggt gaccacccag
     1861 tacgtcggcg aggccgagta ctgcgacgag gtggcgctgc tggcagaggg agagctggtc
     1921 gcgctggatg cgccagaggc gctgcgccgc cacgtgtacg ggggcgatgt gctcgagatc
     1981 gataccgacc gggccgtgga cccggaggtc gtggcgcccg tccgagaggt gatggaggtt
     2041 cgccagcctg cgccacgccg cctgatcgtg gtggtgccag atgcgggaac cgcaacgccg
     2101 cgcctgatgg acgccctcca gcagcgcggc atctcggtgg ttgccatcga ggagtaccag
     2161 ccgacctttg acgaggtctt caccgaccta gtcaaccgcc gccgccgcga acgagaggaa
     2221 gaggcgaacg gcgcggctcc tggaggcaac catgctgcgt agcctgtggc gcagcctggt
     2281 cagcatcacc tccttcctgg gcaaggagct gcgcgaggtc atccgccgcc cgggtgtgct
     2341 ggccagcctg atccttggcc cgttcctgat catggcgttg ttcggcatcg gctatacggg
     2401 caaccgttcg ccgttcaaaa ccgagatcgt ggtgcccgag aactcgcagc tctcccgtga
     2461 tccggagttc tacgccgacc tggccccggg ccgcctggac gtggtgggcg tcaccgccga
     2521 ccgcgccgca gcagaggagc ggttgcgggc caaggagacc gatctgctgg tcgtggcacc
     2581 cgaggatgcc gcggcgcagc tgcgcgccgg cgagcagacc gagatcggcg tggcctggaa
     2641 cgagatcgac ccggtctacg acagcctggc ccacctggcg gtggccacca tggtcagcga
     2701 gctgaac
//
LOCUS       DATACA010000500         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_549240, whole genome shotgun sequence.
ACCESSION   DATACA010000500 DATACA010000000
VERSION     DATACA010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549240"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..402
                     /locus_tag="VHK28_10125"
     CDS             28..402
                     /locus_tag="VHK28_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX2142607.1"
                     /translation="MKMRYVMLTYVDPADIAIWEGWTPEEQAADVDRHRAWFAKFRDK
                     VVSGEELDEPKHVKSLRRGRQDEGVVVIDGPYVETKELLGGFVVIEAADMDEAIAIAS
                     EWPSLSSMPNATVQVQPVFVRD"
     gene            complement(407..1321)
                     /locus_tag="VHK28_10130"
     CDS             complement(407..1321)
                     /locus_tag="VHK28_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX2142608.1"
                     /translation="MSITIVVGIVLGSIGGLFLGGVLNGAPGGPGPGMPDGSPTVSPA
                     PDPTATPRPSATPTPTGSPTPSPTPSPTPTVAPTLVPTPQPTARPTLAPTPVPPPPSG
                     PPACRYDDLLTFHRDLGSWRISLLDTIYMLPGSYAPGDLVDSSVVGLNGGYPIRQIVV
                     ADLARMVADARAAGAPLGVASGYRSHAQQTSTFQYWVNQVGYEAALRTSARAGHSEHQ
                     LGTTIDFKSAGGAAPWEYADWATTPAGAWMAANAWRYGFVMSYPRGSFAQTCYDYEPW
                     HYRYVGRELAAQIRASGLTPRQVLWTLQ"
     gene            1551..1685
                     /locus_tag="VHK28_10135"
     CDS             1551..1685
                     /locus_tag="VHK28_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142609.1"
                     /translation="MHQRRGPATLRPGLARLALAVGLVAAATPGTVCRNGIRASHPRP
                     "
BASE COUNT          255 a          585 c          677 g          239 t
ORIGIN      
        1 tgcgcgcccg ccggcgacta gcgaggaatg aagatgcgct acgtgatgct cacctacgtg
       61 gatccggccg acatcgcgat ctgggagggc tggacgcccg aggagcaggc ggccgacgtg
      121 gatcgccacc gggcctggtt cgccaagttc cgcgacaagg tggtttccgg ggaggagctc
      181 gacgagccga agcacgtcaa gagcctgcga cgcggacgcc aggacgaagg cgtggtggtc
      241 atcgacgggc cgtacgtcga gaccaaggag ctgctcggcg gcttcgtggt gatcgaggcc
      301 gccgacatgg acgaggcgat cgccatcgca tccgagtggc cctcgctcag cagcatgccg
      361 aacgccaccg tccaggtgca gccggtcttc gtgcgcgact gaccggctac tgcagcgtcc
      421 agagcacctg ccgcggggtg aggccgctgg cgcggatctg ggcggcgagc tcgcgaccga
      481 cgtagcggta gtgccagggc tcgtagtcgt agcaggtctg cgcgaacgag ccgcgggggt
      541 agctcatcac gaacccgtag cgccaggcgt tggcggccat ccaggcgccg gccggcgtgg
      601 tcgcccagtc ggcgtactcc cacggcgccg cgccgccggc cgacttgaag tcgatggtcg
      661 tgcccagctg gtgctcgctg tgaccggcgc gggcgctggt gcgcagcgcc gcctcgtacc
      721 cgacctggtt gacccagtac tggaaggtgg acgtctgctg cgcgtggctg cggtagccac
      781 tggccacgcc cagcggggcg ccggcggcgc gggcatcggc caccatgcgg gccaggtcgg
      841 cgaccacgat ctggcggatc gggtagccgc cgttgaggcc gaccacgctc gagtcgacca
      901 ggtcgcccgg ggcgtagctg ccgggcagca tgtagatcgt gtccagcagg ctgatacgcc
      961 agctccccag gtcacgatgg aaggtgagca ggtcgtcgta gcggcaggcc ggtgggccag
     1021 agggaggtgg gggcaccggc gtcggcgcca gcgtcggccg agccgtcggc tgcggtgtgg
     1081 gaaccagtgt gggcgcgacg gtcggcgtcg ggctgggcgt cggcgaaggc gtgggggagc
     1141 cggtcggtgt gggcgtggcc gacggccggg gcgtggcggt tggatccgga gcgggcgaga
     1201 cggtgggcga accatccggc atccccggtc cggggcctcc cggggcccca ttcagcacgc
     1261 cgcccaggaa gagtccgccg atcgagccga gcacgatgcc gaccacgatg gtgatgctca
     1321 tcagcaggcg cccagacggg gtccggcgcg gctgcaggga tcgggttgca atagccatgc
     1381 tgcgatgctc ggacgcccgg gttcggaggg catcgcgcga gtgtcgcccc cgaggcggga
     1441 catccggccc gctcgccgag tggccgtttg tctcggggaa cccaaaggga cgccgccacc
     1501 gacccgaatt ggctacccac cagccgccgg cgcccgccta gacttggccg atgcatcagc
     1561 gccgcggtcc agccacgctc cgccccgggc tggcgcgtct ggccctggcc gtcggactgg
     1621 tggcggcggc cacgcccgga acggtgtgca ggaacggcat ccgcgcatct catccgcgcc
     1681 cgtagactgc cgccatgcga gagcggggga tcgcgggccc gagccttcca accgtccgcg
     1741 ccttcatggc gcttgc
//
LOCUS       DATACA010000501         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_150364, whole genome shotgun sequence.
ACCESSION   DATACA010000501 DATACA010000000
VERSION     DATACA010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150364"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VHK28_10140"
     CDS             <1..349
                     /locus_tag="VHK28_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142610.1"
                     /translation="EFFSALLSFVGAHDDIYGWNLWAFVQGLGGTPWDSGAAAILTII
                     VGGAALVGLVLVRWRSLTGAVLGGVIATQLVMFAARWTTYAYFGLVMPVAMALPLLFA
                     WERRGPQANEAVA"
     gene            346..>1611
                     /gene="carB"
                     /locus_tag="VHK28_10145"
     CDS             346..>1611
                     /gene="carB"
                     /locus_tag="VHK28_10145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2142611.1"
                     /translation="MTAPSKVLVIGSGPILIGQAAEFDYAGVQACLALRAEGVETVLV
                     NSNPATVMTDPDVGGTVLIGPLSVDYVERVITEHRPDALLPTLGGQTGLNLAVALDDA
                     GVLERHGLRVLGTPLAAVRAAEDRGGFRNLVTGIGEPVPESAVVESLEDGLAFVRHLA
                     APVVVRPAFTLGGGGGGFAMSEQEARQRIATGLAASPIGQVLVERSLLGWYEIEFEVI
                     RDGADTAIAICGMENFDPMGVHTGDSIVVAPIQTLPDPLVQRLRRCALKIVRALGLEG
                     GCNVQLAVAPDGSDYRVIEVNPRVSRSSALASKATGYPIARVAALVALGKRLHELPNP
                     ATGGNSAAFEPAVDYVVVKIPRWPFDKFPAADRSLGVQMKATGEAMAIDREFGSALLK
                     AVRSLEPRGHGWLWEDAEWGLSHRSPDDLE"
BASE COUNT          211 a          537 c          601 g          262 t
ORIGIN      
        1 cgagttcttc tcggcgctgc tgagcttcgt gggcgcgcac gacgacatct acggctggaa
       61 cctgtgggcc ttcgtgcagg ggctgggtgg cacgccatgg gattcggggg ctgccgcgat
      121 cctgaccatc atcgtgggcg gggcggcgct ggtcgggctg gtcctggttc gctggcgctc
      181 gttgaccggc gccgttctcg gtggcgtgat cgccacgcag ctggtgatgt tcgccgcgcg
      241 ctggacgacc tatgcctact tcgggctggt gatgccggtg gcgatggcgc tgccgctgct
      301 cttcgcctgg gagcgccgtg gcccgcaggc gaatgaggcc gtggcatgac ggcgcccagc
      361 aaggtcctgg tcatcggatc gggaccgatc ctcatcgggc aggcggccga gttcgactac
      421 gccggcgtgc aggcctgcct ggcgctgcga gcggagggcg tggagacggt gctggtcaac
      481 tcgaacccgg ccacggtgat gaccgacccg gacgtgggag gaaccgtgct catcggtccg
      541 ctgtcggtgg actacgtgga acgggtgatc accgagcatc gcccggatgc tttgctgccg
      601 accctcgggg gccagaccgg cctcaacctg gcggtcgccc tggacgacgc gggcgtgctc
      661 gagcgccacg gcctgcgggt gctcggaacg ccgctggccg ccgtgcgtgc ggcggaggat
      721 cgggggggct tccgcaacct ggtgaccggc atcggagagc cagtcccgga gagcgccgtg
      781 gtcgagtctc tggaggacgg cctggcgttc gttcgccacc tggccgcgcc agtggtggtg
      841 cgcccagcct tcaccctggg cggcgggggt ggtgggttcg ccatgagcga gcaggaagcc
      901 aggcagcgga tcgcgaccgg cctggcggcc agtcccatcg gccaggtcct ggtcgagcgc
      961 agcctgctgg gctggtacga gatcgagttc gaggtgatcc gcgatggcgc cgacacggcc
     1021 atcgccatct gcggcatgga gaactttgat ccgatgggcg tgcacaccgg ggactcgatc
     1081 gtggtggccc ccatccagac cctgccggac ccgctcgtcc agcggctgcg acgatgcgcc
     1141 ctgaagatcg tccgcgcgct cgggctggaa ggcggctgca acgtccagct ggcggtggcg
     1201 cctgacggct cggactaccg cgtcatcgag gtcaacccgc gtgtctcccg ctccagcgcc
     1261 ctggcatcca aggccaccgg ctacccgatc gcccgcgtcg ccgccctggt cgcgctcggc
     1321 aaacggctgc acgagctgcc caacccggcc accggtggga acagcgccgc cttcgagccg
     1381 gcggtcgatt acgtggtggt caagatcccg cgctggccct tcgacaagtt ccccgcggcg
     1441 gatcgttcgc ttggcgtcca gatgaaggcc accggcgagg cgatggccat cgaccgcgag
     1501 ttcggctcgg cgctgctcaa ggcggtccgc tccctcgagc cgcgcgggca cggctggttg
     1561 tgggaggacg cggagtgggg cctcagccat cgctccccgg acgaccttga g
//
LOCUS       DATACA010000502         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1033958, whole genome shotgun sequence.
ACCESSION   DATACA010000502 DATACA010000000
VERSION     DATACA010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033958"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..947)
                     /locus_tag="VHK28_10150"
     CDS             complement(join(82..585,588..947))
                     /locus_tag="VHK28_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611378.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEX2142612.1"
                     /translation="MVGRGELTEQAWAQLAPLLPANGGRGGQWHDHRTVINGMLWRLR
                     TGAPWRDLPARYGPWQTCYDRFVRWRRDGPWDRLLTHVQTRSDAVGATDWEVSIDSTV
                     TRAHQQAAGARRCPSRADKKGLQHPPDEALGRSRGGVTTKLHLSCDGQGRPLSLVLTA
                     GQRHESTQLEAVLDGIRVPRPGRGRPRTRPAHLLADRGYSYPRCRRLLRRRGIAHTIP
                     ERRDQRQQRATRPGRSLPFDAERYKGRNIVERCVNRLKQWRCVATRYDKRAVNYRASV
                     VIVSLMLWLNE"
     gene            1092..1697
                     /locus_tag="VHK28_10155"
     CDS             1092..1697
                     /locus_tag="VHK28_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142613.1"
                     /translation="MPKLLFARPPQDEAEERHIRRLARSRHAPGDWIVRARMIAGSWD
                     GLRTTAIAAELGCHPQTVRERLVRFNAAGLEGLGDRPGAGRKPRLTEEERSRIIAVTK
                     SEPPGRLVWDGHRMVAPRPEEPAHWSLDALAEAAQARGIRVSRSQIRRILQAEGVRWR
                     ATRSWAESTDPEFVPKGPRSSPATPTHRPGRRSSASTNWGR"
     gene            1628..2221
                     /locus_tag="VHK28_10160"
     CDS             1628..2221
                     /locus_tag="VHK28_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX2142614.1"
                     /translation="MVACYTDPPAGATVLCVDELGPVTPRAVPPPPGWSVDGHRLKVP
                     IDYGRGPERTWVYGALRVRDGHAQTLTAPSRNTAGYLALLEAIAADNPTGELVLISDN
                     LASHKSPPIQAWLDEHPRVRQVFLPTGACWLNLIEAWWRVFRREAFAGQSFATGEDIA
                     EGTRAATQRLNRRATPWVWGRPRRPHRRLRRRFVYRL"
     gene            2304..>2541
                     /locus_tag="VHK28_10165"
     CDS             2304..>2541
                     /locus_tag="VHK28_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX2142615.1"
                     /translation="METTEPTIDHIAGWRQGLDEVHRRIAPRFARPEVRVRAGRYLDG
                     LLGRAERKNGWQVAEDLGEPTPDGVQRLLNAARWE"
BASE COUNT          386 a          881 c          888 g          386 t
ORIGIN      
        1 gttggtggag gatatatgtt cgttcgtcgc gaattcttgg agcaccttgc attacggcgc
       61 aattagggcg tgtttgacgg atcattcgtt cagccagagc atgagagaaa cgatgaccac
      121 tgatgcgcga tagttcaccg cccgcttgtc atagcgcgtc gccacacacc gccactgttt
      181 cagccggttc acgcaccgct cgacgatatt ccggcctttg taccgctcgg cgtcgaacgg
      241 gagggaccgg cccggtcgcg tggcccgctg ctggcgctgg tcgcggcgct ccggaatggt
      301 gtgggcgatc ccccgtcggc gcagcagccg acggcagcgc gggtagctgt agccccgatc
      361 ggcgagcagg tgcgccgggc gtgttcgtgg ccggccccgc cccggtcgcg gcacgcggat
      421 gccgtccaga acggcttcga gttgggtgct ctcgtgccgt tggccggccg tcagcaccag
      481 cgacaacggc cgcccctgcc cgtcacagga taggtggagc ttggtggtca cgccgccccg
      541 gctgcgcccg agcgcttcgt ccggcgggtg ctggagcccc tttttgcatc ggcccggctg
      601 ggacaccgcc gcgcaccagc ggcctgctga tgggcacggg tgacggtgct gtcgatgctg
      661 acctcccaat cggttgcgcc caccgcgtcc gacctggtct ggacgtgggt cagcagacgg
      721 tcccacggcc cgtcgcggcg ccagcgcacg aagcgatcgt aacaggtctg ccacggcccg
      781 tagcgggcgg gcagatcgcg ccagggagcg ccggtgcgca gccgccagag catgccattg
      841 atcacggttc ggtggtcgtg ccactgcccg ccgcgcccgc cgttggccgg cagcagcgga
      901 gcgagctggg cccacgcctg ctccgtcagt tcgcctcgtc ctaccatggc acctcgtgtc
      961 tagctcgata atcatggccg acatccggcc tgattctacc ggctttatat tcgtcagaca
     1021 cgccctagtg ctccattcct caataaaggt atagaatggg atgatcgagc gaccttcaca
     1081 aggaggcgat catgcccaag ctcttgttcg cccgtccgcc acaggatgag gcggaagaac
     1141 gccacatccg acggttagcc cggagccgcc acgcgccggg tgactggatc gtccgagcgc
     1201 gcatgatcgc cggcagctgg gacgggctgc gcaccaccgc catcgcggcc gagctgggct
     1261 gccaccccca gacggtgcgc gaacggctgg ttcgcttcaa tgctgcgggg ctcgagggcc
     1321 tcggcgaccg ccccggcgcc ggccgcaagc cgcgcctcac cgaggaggag cgcagccgga
     1381 tcatcgccgt gacgaagagc gagccgcccg gccgcctcgt ctgggacggt caccggatgg
     1441 tcgcgccccg gccggaggag ccggcccatt ggtcactcga cgccctggcc gaggcggccc
     1501 aggcgcgcgg catccgtgtc agccgcagcc agatccggcg catcctgcag gccgagggcg
     1561 tgcgttggcg cgcgacccgg tcctgggcag agagcaccga cccggagttc gtcccaaaag
     1621 gaccgagatc gtcgcctgct acaccgaccc accggccggg gcgacggtcc tctgcgtcga
     1681 cgaactgggg ccggtgacac cgcgcgccgt cccgccgcca ccgggctggt cggtggatgg
     1741 gcaccgcctc aaggtgccga tcgactacgg ccgcgggccg gagcgaacct gggtctacgg
     1801 ggcgctgcgg gtgcgggatg ggcacgccca gacgttgacg gcgccgtcgc gcaacaccgc
     1861 cggctatctg gccctgttgg aggcgatcgc ggctgacaac ccgactgggg agctggtgct
     1921 gatcagcgac aacttggcca gccacaagag cccgccgatc caggcgtggc tggacgagca
     1981 cccgcgcgtg cgacaggtgt tcctgccgac gggagcgtgc tggctgaacc tgatcgaggc
     2041 ctggtggcgg gtcttccggc gcgaggcctt cgctgggcag agctttgcga cgggcgagga
     2101 catcgccgaa gggacgcgcg cggcgacgca gcggctcaat cgccgggcca caccgtgggt
     2161 gtggggacgc ccgcgccgtc cgcatcgccg cctccgccgc cgttttgtct accgcctttg
     2221 aggaatggag cactagtact acaaccgcag ttcgtcggct agactcctcc ctgacacgga
     2281 aggagcgtac ccacatggtg cccatggaga cgaccgagcc gaccatcgac cacatcgccg
     2341 gctggcggca gggactggat gaggtccacc gccgcatcgc cccgcgcttc gctcgccccg
     2401 aagtgcgcgt ccgcgctggc cgctacctgg acggcctgct cggccgcgcc gagcgcaaga
     2461 acggctggca ggtggccgag gacctggggg agccgacacc tgatggagtg cagcgcctgc
     2521 tgaacgccgc ccggtgggag g
//
LOCUS       DATACA010000503         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1515023, whole genome shotgun sequence.
ACCESSION   DATACA010000503 DATACA010000000
VERSION     DATACA010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515023"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VHK28_10170"
     CDS             <1..1418
                     /locus_tag="VHK28_10170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019498023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142616.1"
                     /translation="AGIPSFAYPDAAVRMFNYLWRFADNLRLLYETPVLPGDLGGHAS
                     RTAIRSVVEGAARDSRTLLTEVESKQVLAAYGIPVTETRVAATEDEAVAAAQAIGYPV
                     VAKLYSRSITHKTDVGGVKLNLRDADAVRGAHRSIRDAVVDRAGTDAFEGITIQPMIN
                     YTGYELIIGSSVDAQFGPVLLFGLGGTLVEVIRDRALALPPLTTTLARRMIERTRVAK
                     ALAGVRGRRPVGMGELEQLLVRFSRLVVEVPRIAEVDINPLLASAEGLLALDARVVLH
                     PDSVADDELPRPAIRPYPMQYTGEWTAADGTRFTIRPIRPEDEPLMVRFHGELSEESV
                     YARYFTYLKLSQRTAHERLTRICFIDYDREMALVAEARDPSGGEPVIAGVGRLSKAHG
                     RNEAEFAILVADRSQRRGLGTELLRRLVAVGRDEGLDRIFAEMLASNGGMRRTSQAVG
                     FELRPQPDDQQVVLAELSLDR"
     gene            complement(1428..3161)
                     /locus_tag="VHK28_10175"
     CDS             complement(1428..3161)
                     /locus_tag="VHK28_10175"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX2142617.1"
                     /translation="MGEDTTTGGLDDDPGTPLRAPAETVRTDLRGKDLIGNPLLNKGT
                     AFSDDERHAFGLLGLLPPHSTTIEEQVALELEHVRRKPDDLEKYIGLAATQDRNETLF
                     YRLLCEHMEEFLPIVYTPTVGRACQEFSHIFRRPRGVWITPDDVERVDEILRAATSED
                     VRLIVVTDNERILGLGDQGCGGMLIPVGKLSLYTAAAGIHPSWTLPVSLDVGTDRREL
                     RDDPLYLGYRAPRLRGAAYDEVVEAFVEAVRRVFPRALLQWEDFKQHNALRILDRYRD
                     RLPSFNDDVQGTAAVVLAGLLAARRERGGLAGERILSLGAGAAAIGISALVVRQLVSE
                     GLDEREAHERILLMDSKGVLHTNRESLDDNHRPFALDAARLGALGMQSTDLRDPAAVA
                     RAFGPTVLLGTTGHPGAFTRELVRAMADADPPPVIMPLSNPSDRAEATPVQLMEWTDG
                     RAMVATGSPFPEVVTRAGTRVVGQANNVFIFPGVGLGAALAQARRLPEEAFLVAAREL
                     AGLVSAERLAAGAIYPSVADLRPVARAIAVALVRHLRGAGLGAQIPDDAVEAAVEAAM
                     WWPRYPRLEPA"
     gene            3201..4286
                     /gene="queG"
                     /locus_tag="VHK28_10180"
     CDS             3201..4286
                     /gene="queG"
                     /locus_tag="VHK28_10180"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX2142618.1"
                     /translation="MTTKAAARTRLAQLAARHGLQLVAVTDASPLSEDRVRMEDSVAA
                     GRMARMEWMGGQRPATATDPGAHDPEARSVLVVAAPYDGGRDAWDPNPDALRRALAPV
                     LAGRPSQPAGRVARYALGTDYHTALRARLEALAADLRASGLPAGEVAYVDDRPLAERA
                     LAARGGIGWIGKNTNLLTHQKAGSWVFLGALLTSAELPADEPLRTTCGSCTRCLSGCP
                     TGALIGPQTIDARRCISYLTIEHAGVLDAWEATAIGNWIFGCDVCQEVCPVNAEADDP
                     GPLNVPLLPLIEWLLPLGTRAFGRAVGETALTRAGRHRLLRNAIAALANAGPMSPDAR
                     ALLRLAARDRREEVRRQAQMALGEVGV"
     gene            4301..4377
                     /locus_tag="VHK28_10185"
     tRNA            4301..4377
                     /locus_tag="VHK28_10185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4335..4337,aa:Pro,seq:ggg)
BASE COUNT          619 a         1546 c         1595 g          625 t
ORIGIN      
        1 cggccgggat cccctccttc gcctacccgg acgcggcggt ccgcatgttc aactacctgt
       61 ggcgcttcgc cgacaacctg cgcctgctgt acgagacgcc ggtgctgccc ggtgacctgg
      121 gcgggcacgc cagccggacc gcgattcggt ccgtggtcga gggcgctgca cgcgacagcc
      181 gcacgctgct gaccgaggtg gagtccaagc aggtgctggc cgcctacggc atcccggtga
      241 ccgagacccg ggtggcggcg accgaggatg aggcggtggc ggccgcccag gccatcggtt
      301 acccggtggt cgccaagctg tactcgcgca gcatcaccca caagaccgat gtgggcgggg
      361 tcaagctcaa tctgcgggat gcggacgccg tgcgtggggc gcatcggtcc atccgcgacg
      421 ccgtcgtcga ccgcgccggg accgatgcct tcgagggcat caccatccag cccatgatca
      481 actacaccgg gtacgagctg atcatcggct ccagcgtgga cgcccagttc ggcccggtgc
      541 tgctgttcgg cctgggcggc acgctggtgg aggtcatccg cgaccgggcg ctggcgctgc
      601 cgccgctgac cacgacgctg gcccggcgca tgatcgagcg cacccgggtg gcaaaggcgc
      661 tggccggggt ccgcgggcgc cggccggtgg gcatgggtga gctggagcag ctgctggtgc
      721 gcttcagccg gctggttgtg gaggtgccac gcatcgcgga ggtggacatc aacccgctgc
      781 tcgcctccgc cgagggcctg ctggcgctgg acgcgcgggt cgtgctccac ccggacagcg
      841 tcgcggacga cgagctgccg cgaccggcga tccgccccta cccgatgcag tacaccgggg
      901 agtggaccgc cgcggacggg acgcgattca ccatccggcc cattcgtccc gaggacgagc
      961 cgctcatggt ccgcttccac ggcgagctga gcgaggagag cgtctacgcc cgctacttca
     1021 cctacctgaa gctctcgcag cggacggcgc acgagcgcct gacgcggatc tgcttcatcg
     1081 actacgaccg tgagatggcg ctggtggccg aggcgcgcga cccttcaggc ggcgagccgg
     1141 tgatcgccgg cgtggggcgc ctgtcgaagg cccacgggcg caacgaggcg gagtttgcga
     1201 tcctggtggc tgatcgctcg cagcgtcgcg gactggggac cgagctgctg cggcgactgg
     1261 tggcggtggg tcgtgacgag ggcctggatc gcatcttcgc cgagatgctg gccagcaacg
     1321 gcggcatgcg acgcaccagc caggcggtgg gcttcgagct ccggccgcag cccgatgacc
     1381 agcaggtcgt cctggccgag ctcagcctgg accgataggc cccgcgccta cgccggctcg
     1441 agccgagggt atcgcggcca ccacatggcc gcctccacgg ctgcttccac cgcatcatcg
     1501 ggaatttgcg cgccgagccc ggcgccacgc aggtgccgca ccagggccac ggcgatggcg
     1561 cgcgccacag gccgcaggtc ggccaccgag ggatagatgg cgcccgccgc caatcgttcg
     1621 gctgaaacca gtccggcgag ctcgcgggcg gcgaccagga aggcctcctc cgggagccgt
     1681 cgcgcctggg ccagcgcggc gcccaggccc acccccggga agatgaaaac gttgttggcc
     1741 tggcctacca cgcgggtacc ggccctggtc accacctcgg ggaacgggct gccggtggcc
     1801 accatggccc gcccatcggt ccattccatg agctgcaccg gcgtggcctc cgcgcggtcg
     1861 ctgggattcg agaggggcat gatgaccggc ggcggatcag cgtcggccat ggctcgcacc
     1921 agctcgcggg tgaacgctcc cggatggccg gtcgtgccca gcagcacggt tggaccgaag
     1981 gctcgagcca ccgcggcggg gtcccgcagg tcggtggact gcatccccag tgcgccgagg
     2041 cgtgccgcgt ccagggcgaa cggtcgatgg ttgtcgtcca gcgactcccg attggtgtgg
     2101 agcacgccct tgctgtccat cagcaggatg cgctcgtgcg cctcgcgctc gtcgaggccc
     2161 tcgctgacca gctggcggac caccagcgcg gagatgccga tggcggccgc accggcaccc
     2221 agcgacagga tgcgctcccc ggccaggccg ccacgctcac ggcgcgccgc gagcagcccg
     2281 gccagcacca ccgccgcggt gccctggaca tcgtcgttga aggaggggag gcggtcacgg
     2341 tagcggtcca ggatgcgcag ggcgttgtgc tgcttgaagt cctcccactg gagcagggcc
     2401 ctggggaaga cgcggcggac agcctccacg aacgcctcga ccacctcgtc atacgccgcc
     2461 ccgcgcaggc gcggcgcccg gtagccgagg tagagcggat cgtcccgaag ctcgcgccgg
     2521 tccgttccga cgtcgagcga gaccggcagc gtccaggacg ggtggatgcc ggccgcagcc
     2581 gtgtacagcg acagcttgcc gaccgggatg agcatgccgc cgcagccctg gtcaccgagc
     2641 cccaggatgc gctcgttgtc ggtgacgacg atcagccgca cgtcctcaga cgtggcggcg
     2701 cgcaggatct cgtccacgcg ctccacgtcg tccggcgtga tccacacgcc acgaggccgg
     2761 cggaagatgt ggctgaactc ctggcaggcg cggccgaccg tcggcgtgta gacgatcggc
     2821 aggaactcct ccatgtgctc gcacaggagc cggtagaaga gcgtctcgtt gcgatcctgg
     2881 gtggcggcca ggccgatgta cttctcgagg tcgtccggct tgcgccgcac gtgctccagc
     2941 tcgagtgcca cctgctcctc gatggtggtc gagtgcggtg gcagcaagcc gagcaggccg
     3001 aacgcgtggc gctcatcgtc gctgaacgcg gtccccttgt tcagcagggg attgccgatc
     3061 aggtcctttc cgcgcagatc ggttcgcacc gtctcggccg gtgcccgcag cggcgtccct
     3121 ggatcgtcgt cgaggccgcc ggtggtcgtg tcctcgccca ttcgcgcaca gggtacggtg
     3181 ctgggccatc atgaggtgtc atgactacga aggcagccgc ccgcaccagg ctcgcacagc
     3241 tggccgcgcg ccacgggctg cagctggtcg cggtcaccga tgcctcaccc ctgtcggagg
     3301 accgcgtgcg gatggaggac agcgtggccg ccgggcgcat ggcgcgcatg gaatggatgg
     3361 ggggccagcg gccggccacc gccaccgatc cgggtgcgca cgatcccgag gcgcggtcgg
     3421 tcctggtggt cgccgccccg tacgatggcg ggcgcgatgc ctgggatccc aacccggacg
     3481 ccctgcggag ggcgctggct ccggtgctgg ccgggagacc gtcgcagccg gccgggcggg
     3541 tcgcgcgtta cgcgctgggg accgactacc acacggccct gcgcgctcgg ctcgaggccc
     3601 tggccgcgga cctgcgcgca tcgggtctcc cggccggcga agtcgcctac gtcgacgacc
     3661 gtccgctggc ggagcgcgcc ctggcggcgc ggggcggcat cggctggatc gggaagaaca
     3721 ccaacctgct gacccaccag aaggccggct cgtgggtctt cctgggggcg ctgctgacct
     3781 cggccgagct gccggccgac gagccgctgc gcaccacatg cggctcctgc acgcggtgcc
     3841 tgtccggctg tccgaccggc gcgctcatcg gtccgcagac catcgacgcc cgtcggtgca
     3901 tcagctacct caccatcgag cacgcggggg tgctggatgc gtgggaggcc acggccatcg
     3961 gtaactggat cttcggctgc gacgtgtgcc aggaggtctg cccggtcaac gccgaggccg
     4021 acgaccccgg ccctttgaac gtaccgctgc tgcccctcat cgagtggctg ctgccactcg
     4081 gcacccgcgc cttcggccgg gcggtggggg agacagcatt gacccgcgcg ggacgtcacc
     4141 gtttgctgcg caacgccatc gcggcgctag ccaacgccgg accgatgtct cccgatgcgc
     4201 gggctctcct gcggctggcc gcccgggacc ggcgggagga ggtccgccgg caggcgcaga
     4261 tggccctcgg cgaggtcggc gtgtagactt accgcacggt cggggcgtgg cgcagtctgg
     4321 cagcgcactt ggctgggggc caaggggtcg gaggttcgaa tcctctcgcc ccgaccacta
     4381 cgctg
//
LOCUS       DATACA010000504         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1755730, whole genome shotgun sequence.
ACCESSION   DATACA010000504 DATACA010000000
VERSION     DATACA010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755730"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VHK28_10190"
     CDS             complement(<1..549)
                     /locus_tag="VHK28_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2142619.1"
                     /translation="MPEPTTRGVRQRFAPSAVNYHALRILGPRLPRLARGWMPWVGLE
                     RYPPPPLPGPDWVRLRPLLSGVCGTDISLLTGHASAVLSPFASFPAVLGHEVVATVTE
                     SGTAAGVAAGTRVVLDPLLGCTVRGITPPCEWCAAGQPGLCLRTAEGGFAPGPMIGFC
                     SDLPGGWSDEVVAHLSQLHVVPE"
     gene            complement(559..1746)
                     /locus_tag="VHK28_10195"
     CDS             complement(559..1746)
                     /locus_tag="VHK28_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX2142620.1"
                     /translation="MSRPARALPPRDPAGAPVVVHRAAREAPSGVAVVGEATGGLVGW
                     FLEGVTQRLSDRGHPVYRPDGRDAQSIPSDVRVILNAVRSEDPRSFRRRSKDIFVVGL
                     AELTDRPADMLQAGYTLLVRSLSNVFIPMVGDHGVPEAHFITMEQGFHRFGHDGDDGA
                     FFDEVVERLIPLAESRLVIDNEFIPDLPEELWGGDEHTEAIYRAGLRMKELDLLPAPW
                     PIDELLSAEDLRHVKRLFGLGGLSYGNASARHDGDRFWMSASGVDKSHLQQVGTEILL
                     VTGYDPATGVVRLSVPPDVTPRRVSVDAIEHLTIYREHPEVGAILHVHGWVAGAISTE
                     VVYPCGTEELALEVAELVRQAPDPEHAVIGLKNHGLTITGSSMDEILERVGPNIVRRV
                     PMD"
     gene            complement(1743..3110)
                     /locus_tag="VHK28_10200"
     CDS             complement(1743..3110)
                     /locus_tag="VHK28_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2142621.1"
                     /translation="MVPLRRALAVAALLLGMVALAAPAAGAGDHIVRLQLTGVIDQVT
                     ASYMEEGIAAAERSGAAAVLVEIDTPGGELTSTDRIVKAMLGSDIPVITWVSPAGAQA
                     ASAGTFITLAGDVAAMAPSTTMGAAAVVGSGGAELPDTIADKVNNSNAAKLKELARDH
                     GRNVEWAESAVTESASISASEAIELEPPAVDLVAPDVDALLAAIDSGQRADGARLTVN
                     GEQLPALAGLDVRDVDMNIGQQFLHILSDPNIAFILFTIGFYGIISELWHPNFFSGPV
                     GAIALVLAFIGSNSLPLNVGGLLLIALGIGLLALELVVASFGLLTAGGVICILLGAFA
                     LWTGVDPQEDAVQVQVSPWLLVAVIGLSLVYVFGVARALMAMRRQVTPQSPALALVGA
                     GGVAQTLIAPTGVAFAAGEAWSARSRGEEIPPGSPVRVVEVDGLELIVEPGEGNQPEQ
                     GEASS"
     gene            complement(3160..>3563)
                     /locus_tag="VHK28_10205"
     CDS             complement(3160..>3563)
                     /locus_tag="VHK28_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142622.1"
                     /translation="PSTTMGAAAVVGSGGAELPDTIADKVNNSNAAKLKELARDHGPN
                     VEWAEELDADWEPDFRLVGLLNDDSNPVGSVHLGVVFTVEAAGRPVRVRETDKLTGRM
                     PATEDLAAAWDRLETWSQLVAAHLLGLDPPS"
BASE COUNT          546 a         1278 c         1246 g          493 t
ORIGIN      
        1 ctcaggcacg acgtggagct gcgatagatg tgcgactacc tcgtcggacc agccgccggg
       61 gaggtcgctg cagaagccga tcatcggccc gggcgcgaag ccgccttccg ccgtgcgcag
      121 gcagagcccg ggctgcccgg cggcacacca ctcgcacggc ggcgtgatcc cgcgcacggt
      181 gcatcccagc agggggtcga gcacgacccg ggtgccggcc gccacgccgg cagcggtccc
      241 cgattcggta acggtggcga ccacctcgtg gcccagcacg gccgggaacg aggcgaaagg
      301 cgacagcacg gcgctggcgt ggccggtgag cagggagata tcggtcccgc acacgccgga
      361 cagcaggggc cgcagccgga cccagtcagg cccgggaagc ggtggtgggg ggtatcgctc
      421 cagcccgacc catggcatcc agccgcgggc caggcgcggc aggcgaggtc cgaggatgcg
      481 cagcgcgtgg tagttgacgg ccgagggggc gaaccgctgc cggacgcccc gggtggtggg
      541 ctcgggcacg gtgcccggct agtccatggg gacgcggcgg acgatattcg ggccgacccg
      601 ctccaggatc tcgtccatgc tgctgccggt gatggtcagg ccgtggttct tcagcccgat
      661 caccgcgtgc tcgggatccg gcgcctggcg aaccagctcc gcgacctcca aagccagctc
      721 ctcggtcccg caggggtaga cgacctcggt ggagatggcg ccggccaccc agccgtggac
      781 gtggaggatg gcgcctacct ccggatgctc ccggtagatg gtcaggtgct cgatggcgtc
      841 cacagacacg cgtcgcggcg tcacgtcggg cggcaccgac aggcgaacca cgccggtggc
      901 cgggtcgtag ccggtgacca gcaggatctc ggtgcccacc tgctgcaggt ggctcttgtc
      961 caccccgctg gcgctcatcc agaagcggtc gccgtcgtgc cgcgcgctgg cgttgccgta
     1021 ggacagcccg ccgagcccga acaatcgctt gacgtggcgc aggtcctccg cggacagcag
     1081 ctcgtcgatg ggccacgggg ccggcagcag gtccagctcc ttcatgcgca gtccggcgcg
     1141 gtagatggcc tcggtgtgct cgtcgccgcc ccacagctcc tcggggaggt ccgggatgaa
     1201 ctcgttgtcg atcaccagcc gtgactcggc cagcgggatc agccgctcca cgacctcgtc
     1261 gaagaaggcg ccgtcgtcac cgtcgtgacc aaagcggtgg aagccctgct ccatggtgat
     1321 gaagtgcgcc tccggcacgc cgtgatcgcc gaccatgggg atgaacacgt tcgacagcga
     1381 gcggaccagc agggtgtatc cggcctgcag catgtccgca ggccgatccg tcagctcggc
     1441 gaggcccacc acgaagatgt ccttgctgcg acggcggaag ctgcgcgggt cctccgagcg
     1501 caccgcgttg aggatgacgc gcacgtcgga cgggatggac tgcgcgtcgc ggccgtcggg
     1561 ccggtagacc ggatggcccc gatcgctcaa ccgctgggtc acgccctcca ggaaccagcc
     1621 caccaggcca ccggtcgcct cgcccaccac cgccaccccg ctcggtgcct ccctggccgc
     1681 gcggtgcacg accaccgggg cgcctgcggg atcccgcggc ggcagcgcgc gcgcagggcg
     1741 gctcaagacg acgcctcgcc ctgctcgggc tggttgccct cccccggttc cacgatcagc
     1801 tccaaaccat cgacctccac tacgcgcacc ggggaccccg gcggtatctc ttctccccgg
     1861 ctccgggcgg accacgcttc gccggcggcg aaggcgaccc cggtgggtgc gatcaatgtc
     1921 tgtgccacgc ccccagctcc caccagcgcc agcgcgggac tctgtggcgt cacctggcgg
     1981 cgcatggcca tcagggcgcg tgccacgccg aagacgtaca ccagcgaaag accgatgacc
     2041 gcaaccagca gccatgggct gacctgcact tgaaccgcgt cctcctgcgg gtcgacgccg
     2101 gtccacagcg cgaaggcgcc cagcaggatg cagatcaccc caccggcggt cagcaggccg
     2161 aacgaggcga ccaccagctc cagcgccagc agaccgatgc ccagcgcgat cagcagcagc
     2221 ccaccgacgt tcaacggaag gctgttggaa ccgatgaagg ccagcactag cgcgatggcg
     2281 ccgaccggcc ccgagaagaa gttcgggtgc cacagctcgc tgatgatccc gtagaagccg
     2341 atggtgaaca ggatgaaggc gatgttcggg tccgacagga tgtgcaggaa ctgctggccg
     2401 atgttcatgt ccacgtcgcg cacgtccagc ccggcgaggg ccggcagctg ctccccgttg
     2461 acggtgagcc gcgcgccgtc ggcacgctgg ccggagtcga tggcggccag cagcgcgtcc
     2521 acgtcgggcg ccaccaggtc gaccgccggc ggctccagct cgatcgcctc gctggccgag
     2581 atgctggccg actctgtcac cgcgctctcc gcccactcca cgtttcggcc gtgatcccgg
     2641 gcgagctcct tgagcttggc ggcgttggag ttgttgacct tgtcggcgat ggtgtccggc
     2701 agctccgctc cgccgctgcc gaccacggcc gcggcgccca tagtggtgga gggcgccatg
     2761 gccgcgacgt ccccggcgag ggtgatgaac gtccccgcac tggcggcctg cgccccggcc
     2821 ggcgacaccc atgtgatgac cggtatgtcg ctgccgagca tggccttcac gatccggtcg
     2881 gtgctggtca gctcgcctcc tggcgtgtcg atctccacca gcacagcggc ggcgccgctg
     2941 cgctcggcgg cggcgatgcc ctcctccatg taggaagcgg tgacctggtc gatcacgccg
     3001 gtgagctgca ggcgcacgat gtgatcaccc gcacccgcag ccggcgccgc gagggcgacc
     3061 ataccgagga gcagggcggc gaccgcgagc gcgcgccgca acgggaccat gaccggagta
     3121 tacgcaggct tcccccggat gccccgcggc ccgactcggt caggaaggcg ggtccagccc
     3181 caggagatgt gccgctacca gctgcgacca ggtctcgagg cgatcccagg cagccgcgag
     3241 gtcctcggtg gcgggcatgc ggccggtcag cttgtccgtc tcgcgcaccc tcaccggcct
     3301 tccggcggcc tccacggtga acacgacacc gaggtggacc gatccaaccg ggttggagtc
     3361 gtcgttgagg aggcccacca ggcggaagtc aggctcccag tcagcgtcga gctcctccgc
     3421 ccactccacg tttgggccgt gatcccgggc gagctccttg agcttggcgg cgttggagtt
     3481 gttgaccttg tcggcgatgg tgtccggcag ctccgctccg ccgctgccga ccacggccgc
     3541 ggcgcccata gtggtggagg gcg
//
LOCUS       DATACA010000505         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1034232, whole genome shotgun sequence.
ACCESSION   DATACA010000505 DATACA010000000
VERSION     DATACA010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034232"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VHK28_10210"
     CDS             <1..362
                     /locus_tag="VHK28_10210"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX2142623.1"
                     /translation="ELLAALDAGAWQPRTRLLGPFDPLIADRERTEELFGFRYKFELY
                     VPAAKREFGPYTLAVLHGERLLGRVVAAVDRRARRLVVERIVPEADAPPETADLARGA
                     LADLAAWLGVEVELGDR"
     gene            complement(532..987)
                     /locus_tag="VHK28_10215"
     CDS             complement(532..987)
                     /locus_tag="VHK28_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142624.1"
                     /translation="MRPLQLAVLLLLVASCEATSPPLNTHAAHDPQAERVKASLADAF
                     GMRFESAGPHHELGKTDDGVQLDLVGVPVEQVVLSVPDDDAAAGEALMPHIRDLLHGP
                     PRVYEWAEDALACHAAGNGCEQRFEQGNLEARVTSEGGFVVLTVSRGSG"
     gene            complement(993..2477)
                     /locus_tag="VHK28_10220"
     CDS             complement(993..2477)
                     /locus_tag="VHK28_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2142625.1"
                     /translation="MLRGRGLRLTDVPAGHSPAGHTYDAVVVGAGPNGLSAAITLARA
                     GKSVLVLEAKPTVGGGSRSSALTLPGFLHDVCSAVHPLGRGSPAFARWPLDEHGLEWI
                     EPPVAIAHPLDDGSAAVVRRNLEATAAQFGEDAERYRRLAGPVARDWDLIARTMLGPL
                     DLRGPLRHPIALGRFGLNAIQPASWLAGRFRTPRLRALLAGIAAHSFLRLDQPITGSF
                     VLAFLGSAHAVGWPIPRGGSQRIADALASYLQSLGGEIRTGQRVTDLAELPPHRAVLL
                     DLTPRQVLAVAGDRLGGSYRRQLRRYRYGPGVFKVDLALDGPVPWTNPELQEAGTVHL
                     GGTFEEIAASERAVARGRHAERPFTLVAQPSLFDPSRAPEGKHVLWAYCHVPNGSTVD
                     MTEPILAQLERFAPGVRKRIIGRSTMDTAAVEAYNANYVGGDINGGIQDLAQLWTRPA
                     IRLDPYSTPDESIFICSSSTPPGGGVHGLCGWHAARSALDGVLK"
     gene            complement(2450..2722)
                     /locus_tag="VHK28_10225"
     CDS             complement(2450..2722)
                     /locus_tag="VHK28_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX2142626.1"
                     /translation="MTSWGDEVACTHADNLPPVEPSGSGCKECLATGGTWVHLRLCLH
                     CGHVGCCDQSPGRHATAHYREQEHPVVQSFEPNEDWRWCYADEVYG"
     gene            complement(2719..3603)
                     /locus_tag="VHK28_10230"
     CDS             complement(2719..3603)
                     /locus_tag="VHK28_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX2142627.1"
                     /translation="MGPIEQLGTALAGVAETAGRSTVGIGNRWRGGSGVVIENGKVLT
                     NAHNLHGEEVRVFFPDGREAEGRVMGADPEGDLAVIGVDTGESPAVEWGDGATLGIGS
                     PVVAVGNPAGRGVRVTLGFVSGVGRSFRGPRGRRVAGAVEHTAPLLPGSSGGPIVDVD
                     GKLLGINTNRLGSGFYQAIAADASLRERASALGRGEAPARRRLGVGLAPSHVARQLRR
                     AVGLDERDGLLVRDVEEGSPAEKAGIAEGDLIVAAAGRPVATADDLFDAIGSVAADAT
                     LELTVLRGAEERAVSISG"
     gene            3701..>3927
                     /locus_tag="VHK28_10235"
     CDS             3701..>3927
                     /locus_tag="VHK28_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142628.1"
                     /translation="MTSAPQSPLAAALERVGDRWSLLLVEALLQGPRRFGELGESVPG
                     IAPNILADRLRRLEAERVVRSQPYSERPPRFV"
BASE COUNT          550 a         1442 c         1410 g          525 t
ORIGIN      
        1 ccgagctgct ggccgcgctc gatgccggcg cgtggcagcc gcggacccgc ctgctcggac
       61 cgttcgaccc gctgatcgcg gatcgggagc ggaccgagga gctgttcggc ttccgctaca
      121 agttcgagct gtacgtgccg gccgccaaac gcgagttcgg cccgtacacg ctggccgtcc
      181 tccacgggga gcggctgctc gggcgcgtgg tggccgcggt cgaccgccgt gcccgccgcc
      241 tggtcgtgga gcgaatcgtg ccggaggcgg atgccccgcc ggagaccgcc gacctcgcgc
      301 gcggcgccct cgcggatcta gccgcctggc tgggcgtgga ggtcgagctc ggggaccgat
      361 agatcggggc agattgcggg tgagtcactt gccgggtgct cgctgactca tctatcgggg
      421 catccagcac gggaaccggg ccaaaaccgc ctcccgggcg cccagtgcca cgatagatcg
      481 gtcaggcgac cgtccaccgc ccgcgcagtg aatcctgccc cgatagaaag gtcagccgga
      541 gccgcgggac acggtgagga ccacgaagcc gccctcgctc gtcacgcgcg cctccaggtt
      601 tccctgctcg aagcgctgct cgcagccgtt acccgcggcg tggcaggcga gggcgtcctc
      661 ggcccactcg tacacccgtg gcgggccgtg cagcaggtcg cggatgtgcg gcatgagggc
      721 ctcgccggcg gccgcgtcgt catcggggac gctgagcacc acctgctcca ccggaacgcc
      781 gacgaggtcc agctgcacgc cgtcatcggt cttgccgagc tcgtggtggg gaccggctga
      841 ctcgaagcgc atgccgaagg catcggccag tgaagccttc acccgctcgg cctgcgggtc
      901 gtgcgccgcg tgcgtgttca gcggcgggct ggtcgcctca cagctggcca ccagaagcag
      961 caggaccgcg agctgcagcg ggcgcatcgg tctcacttga gcacaccgtc cagcgcggaa
     1021 cgggccgcgt gccagccgca caggccatgg acgccgccgc cggggggcgt cgacgacgag
     1081 cagatgaaga tcgactcgtc cggcgtggag taggggtcca ggcggatggc cgggcgggtc
     1141 cacagctgcg ccaggtcctg gatgccgccg ttgatatcgc cgccgacgta gttcgcgttg
     1201 taggcctcga ccgccgccgt gtccatggtc gagcgaccga tgatccgctt gcgcaccccg
     1261 ggcgcgaagc gttccagctg ggccaggatc ggctcggtca tgtccaccgt cgagccgttc
     1321 ggcacgtggc agtaggccca cagcacgtgc ttgccctcgg gagcgcgcga cgggtcgaag
     1381 agcgacggct gggccaccag ggtgaaaggc cgctcggcgt ggcggccgcg ggccacggcg
     1441 cgctcggaag ccgcgatctc ctcgaaggtg cccccgaggt gcaccgtgcc ggcctcctgc
     1501 agctccggat tggtccacgg aaccgggccg tccagggcga ggtccacctt gaagacgccc
     1561 ggcccgtagc ggtagcgacg cagctggcgc cggtacgaac ccccaagccg gtcaccggcc
     1621 acggccagca cctggcgcgg cgtgaggtcc agcagcaccg cgcgatgcgg cgggagctcg
     1681 gccaggtcgg tcacgcgctg gccggttcgg atctcgccgc cgagcgactg caggtaggag
     1741 gccagggcgt ccgcgatccg ctgcgagcca ccgcgcggaa tcggccatcc gaccgcgtgc
     1801 gcgctgccca ggaaggcgag cacgaacgat ccggtgatcg gctggtcgag gcgcaggaag
     1861 gaatgggcgg cgatgccggc cagcagggcg cgcagccgcg gcgtccggaa gcgacccgcg
     1921 agccagctgg ccggttggat ggcgttcagg ccgaagcggc ccagcgcgat cgggtgacgg
     1981 agcggcccgc gcaggtccag ggggcccagc atggtgcggg ctatcaggtc ccagtcgcga
     2041 gccaccggtc cggccagcct ccggtagcgc tcggcatcct cgccgaactg cgcggcggtg
     2101 gcctccaggt tgcggcgcac caccgcggcc gagccgtcgt ccagcggatg cgcgatggcc
     2161 accggcggct cgatccactc caggccgtgc tcgtccagcg gccaccgcgc gaaggccggt
     2221 gacccgcggc ccagggggtg gacggcggag cacacgtcgt gcaggaagcc gggaagggtc
     2281 agcgccgatg agcgcgagcc acccccgacc gtcggcttgg cctccagcac caggacgctc
     2341 ttgccggcgc gggccagggt gatggccgcc gacagcccgt tcggcccggc gcccaccacc
     2401 accgcgtcat aggtgtggcc cgctgggctg tggccggctg gcacgtcggt cagccgtaga
     2461 cctcgtccgc gtagcaccag cgccagtcct cgttcggctc gaaggactgc acgaccgggt
     2521 gctcctgctc ccggtagtgc gcagtggcgt ggcgcccggg cgactggtcg cagcagccga
     2581 cgtggccaca gtgcaggcag aggcgcaggt ggacccaggt gccgccggtg gccaggcact
     2641 ccttgcatcc ggagccgctt ggttccaccg gcggcaggtt gtcggcgtgg gtgcaggcga
     2701 cctcgtcgcc ccagctcgtc acccgctaat gctaaccgcg cgctcctctg cgccgcgcag
     2761 gaccgtcaac tcgagcgtgg cgtccgcggc tacagaaccg atggcgtcga acaggtcgtc
     2821 cgccgtggcg accggccggc cggcggcggc cacgatcagg tcgccctccg cgatgccggc
     2881 cttctccgcc ggcgagccct cctccacgtc ccgcaccagg agtccgtcgc gctcgtccag
     2941 gccgaccgcg cggcgcagct gccgggccac gtgcgacggg gccaggccca cgcccaggcg
     3001 ccgacgcgcg ggtgcctcgc cgcggcccag tgccgaggcg cgctcgcgca gggaggcgtc
     3061 cgccgcgatg gcctggtaga agccgctccc caaacggttg gtgttgatac ccagcagctt
     3121 gccatcgaca tccacgatcg gcccgccgga cgatcccggc agcaggggag cggtgtgctc
     3181 caccgcgccg gccacccgac ggccccgcgg gccacggaag gagcgtccga cgccggacac
     3241 gaagccgagc gtgacgcgca cgccgcgacc tgccgggttg ccaaccgcga ccaccggcga
     3301 gccgatgccg agcgttgccc cgtcgcccca ctccacagcc ggcgactccc cggtgtcgac
     3361 gccgatcacc gccaggtcgc cctccgggtc ggcccccatg acgcggccct ccgcctcacg
     3421 accgtccggg aagaagacgc gcacctcctc gccgtgcagg ttgtgcgcgt tggtcagcac
     3481 cttcccgttc tcgatcacca cgcccgatcc gccgcgccat cggttcccga tgccgacggt
     3541 cgaccttccc gccgtctctg ccacgcccgc cagggcggtt ccaagctgct cgatgggacc
     3601 catcggtcca tcctcctgtt gcaacgctag aatgtgactt gcaatgtgca agttacatgc
     3661 atctggccgg ctgtcaagcc cgatgacgta cgatccgcgc gtgacctccg cgccacagtc
     3721 accactggcc gccgcgctcg agcgggtggg ggaccgatgg agcctcctgc tggtcgaggc
     3781 gctgctccaa ggcccgcgcc gtttcggcga gctgggggag tcggtgcccg gcatcgcgcc
     3841 gaacatcctg gccgatcggc tgcgccggct ggaggccgag cgggtggtgc gttcgcagcc
     3901 ctactcggag cgtccgccgc gcttcgt
//
LOCUS       DATACA010000506         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_712095, whole genome shotgun sequence.
ACCESSION   DATACA010000506 DATACA010000000
VERSION     DATACA010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712095"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1255)
                     /locus_tag="VHK28_10240"
     CDS             complement(116..1255)
                     /locus_tag="VHK28_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX2142629.1"
                     /translation="MSLVHAAMEAFPVSVYRSRLRRAADEAGRRGLAALLVTPSSDYG
                     YLLGYRPPALERLTCLVLPASGAPSLLVPRLEAPLARHALGELASEIELLEWDETDDP
                     YRLVRGLLEGNTGRVGVQDQMWARFVLRLRAYLEPMELVEGGGTLAALRRVKTPEEVE
                     RLRAAAAAADQAMLAITGERLSGRSEAEVASRIRDLLLQAGHETADFAIVGSGPNSAS
                     PHHEVGSRVIAAGDAIVLDIGGTRGGYCSDTTRTAFVGEPPAEFGELHAVLLSAQVAA
                     CEAVAPGVAAEDVDAAARDVITDAGYGEWFVHRTGHGIGIETHEEPYIVTGNREPLEP
                     GHAFSVEPGIYLEGRWGARIEDIVVCTPEGGERLNQTSRDLYLVE"
     gene            complement(1252..1716)
                     /locus_tag="VHK28_10245"
     CDS             complement(1252..1716)
                     /locus_tag="VHK28_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2142630.1"
                     /translation="MPDVRGGLTGPLRLPAALAEELLAHAQACLPNEACGLLSAARGS
                     GVATRFHATRNVDASPLRFTVHPEDLVRIVFEIEREGRQLLAIFHSHVRSAAVPSAAD
                     RRAAAAYPDACHVLASMTDASAGPHAALRAWRIHDGEATPVALEIMSGTLAP"
     gene            complement(1692..>2088)
                     /locus_tag="VHK28_10250"
     CDS             complement(1692..>2088)
                     /locus_tag="VHK28_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX2142631.1"
                     /translation="GYGLVVDGSDNLETRYALNDAAVRLRKTVVHASAYRWEAQVTTL
                     VPFDGPCYRCLYPSPPPAELAPDCEVAGVPGVLPGLAGMVQATEALKLLLGVGETLVG
                     RLLMIDALRSEFNEMRVARNPACPTCGAG"
BASE COUNT          289 a          776 c          756 g          267 t
ORIGIN      
        1 gagcgcctct ccgccccctt cctggccgtt ggagccgcca cgtggccatg acctcggctt
       61 cctggccgtt ggagccgcca cgtggccatg acccggctgg ccccgcgggc cgcacctact
      121 cgaccaggta caggtcccgg ctggtctggt tcaagcgctc accgccttcc ggggtgcaga
      181 ccacgatgtc ctcgatccgc gccccccagc ggccctcgag gtagatcccc ggctccacac
      241 tgaaggcgtg ccccggctcc agcggctcgc ggttgccggt gacgatatac ggctcctcgt
      301 gggtctcgat cccgatgccg tgcccggtgc ggtgcacgaa ccactccccg tagccggcgt
      361 cggtgatgac gtcgcgggcg gcggcgtcca cgtcctcggc ggccacgccc ggggccacgg
      421 cctcgcaggc cgcgacctgg gcgctcagca gcacggcgtg cagctccccg aactcggctg
      481 gcggctcacc gacgaacgcg gtgcgggtcg tgtccgagca gtagccgccc cgcgtccccc
      541 cgatgtccag cacgatggca tccccggccg cgatcacgcg tgaaccaacc tcgtggtgcg
      601 ggctggccga gttcggcccg gagccgacga tcgcgaagtc ggccgtctcg tgcccggcct
      661 gcagcagcag gtctcggatg cgggaggcta cctccgcctc gctacggccg gagaggcgct
      721 cgccagtgat ggccagcatc gcctggtccg cagctgccgc cgcggcccgc agccgctcga
      781 cctcctcggg cgtcttcacg cgacgaaggg cggccagcgt cccgccgccc tccaccagct
      841 ccatcggctc caggtaggcg cgcagccgga gcacgaagcg cgcccacatc tggtcctgca
      901 cgccgacccg cccggtgttg ccctcgagga ggccgcgcac caggcggtag ggatcatcgg
      961 tctcgtccca ttcgaggagc tcgatctcgg aggccagctc gcccagcgcg tggcgcgcca
     1021 gtggagcctc cagccgtggg acgagcaggc tcggcgcacc ggaggccggc agcacaaggc
     1081 aggtgagccg ctccagggcg ggcgggcggt agccgagcag gtagccgtag tcggaggacg
     1141 gcgtgaccag cagcgccgcc aacccgcggc gaccggcttc gtccgcggcg cgtcgcaacc
     1201 tggatcggta gaccgatacg gggaaggcct ccatcgcggc atgcaccagg ctcatggcgc
     1261 aagggtaccc gacatgatct cgagcgccac cggggtggcc tcgccgtcgt ggatgcgcca
     1321 ggcgcgcagc gcggcgtggg ggccggcgga ggcatcggtc atggaggcca ggacgtggca
     1381 ggcgtccggg tacgcggccg ccgcccggcg gtcggccgca gacggcaccg ccgccgaacg
     1441 cacgtgcgag tggaagatgg cgagcagctg ccgcccctcg cgctcgatct cgaagacgat
     1501 gcgcaccagg tcctcggggt ggaccgtgaa gcgcagcggc gaggcgtcga cattgcgtgt
     1561 tgcgtggaac cgcgtggcca ctcccgagcc gcgtgcggcg gacaggagcc cgcaggcctc
     1621 gttcggcagg caggcctgcg cgtgggccag cagctcctcg gcgagggcgg ccgggagtcg
     1681 aagggggccg gtcagcccgc cccgcacgtc gggcacgcgg ggttgcgcgc cacgcgcatc
     1741 tcattgaact cgctgcgcag cgcgtcgatc atcagcagcc ggccgaccag cgtctctcca
     1801 acgcccagca gcagcttgag cgcctcggtg gcctggacca tgccggccag cccggggaga
     1861 acgccgggga cgccggccac ctcgcagtcc ggcgccagct cggccggcgg gggcgacggg
     1921 tacaggcagc ggtagcaggg cccatcgaac gggaccaggg tggtgacctg cgcctcccag
     1981 cggtaggcgg aggcgtgcac caccgtcttg cgcagccgca cggccgcgtc gttcagcgcg
     2041 tagcgcgtct ccaggttgtc gctgccgtcc acgaccagcc cgtagccg
//
LOCUS       DATACA010000507         1591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_311331, whole genome shotgun sequence.
ACCESSION   DATACA010000507 DATACA010000000
VERSION     DATACA010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311331"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /gene="murJ"
                     /locus_tag="VHK28_10255"
     CDS             <1..1405
                     /gene="murJ"
                     /locus_tag="VHK28_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX2142632.1"
                     /translation="QLVVAGALSAALIPVFSSYRARGQEAEAWRLASSVINLVVIALA
                     GLSLVMAIFAPLLVPIVAPGFDAPTTELTIRMTRVMLLSPVLIGMGAVVSGILNSYER
                     FAVPSLAPLAYNLAIILAAIFLAPFMGVEGLAVGVAVGSLAHLVIQLPELGRVGQRYD
                     LTIGLSHPGVRKVVWLMGPRMLGLAAGQLNFIVSTVLASGLPEGSITAYNYAFQLSQI
                     PVGVLGVSVAIALFPSLSRDAALGRLPDIRRQVAGSLRILVFLAAPLTAALIVLAEPI
                     ASVFFQYGLFGERSAERTAGALVFFAIGLVGHIVVHVLNRAFYAMQDTRTPVTFAMIA
                     VAINLPLMAWLVGPMGVQGLALALSISSLLEVAALLWALRRRIESIEDGEVARSAARS
                     GVAPGAAALLMLGGLTLVQATLPGLLDHGLGRLVAVAALTSAGGAIYLVVASALRAPE
                     IGQLRALLARRRGGAAA"
     gene            1402..>1591
                     /locus_tag="VHK28_10260"
     CDS             1402..>1591
                     /locus_tag="VHK28_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142633.1"
                     /translation="MIRVRPATDADREAWQAFLERTPAGDFLHDWSWGAVAAFDGQPQ
                     RRFMAEEDGTVVAIVAAQL"
BASE COUNT          178 a          569 c          570 g          274 t
ORIGIN      
        1 ccagctggtg gtcgccggtg ccctgtcggc ggcgctgatc ccggtcttca gcagctaccg
       61 cgcccgcggg caggaggcgg aggcctggcg gctggccagc agcgtgatca acctggtggt
      121 gatcgccctg gccggcctct cgctggtgat ggcaatcttc gcgcccctgc tggtgcccat
      181 cgtggcgccc ggcttcgatg cgccgaccac cgagctgacc atccgcatga cgagggtcat
      241 gctcctcagc ccggtgctga tcggcatggg tgccgtggtc agcggcatcc tcaactcgta
      301 cgagcgcttc gccgtcccgt cgctggcgcc cctggcctac aacctggcga tcatcctggc
      361 cgccatcttc ctggcgccct tcatgggcgt cgaggggctg gcagtgggag ttgcggtcgg
      421 ctcgctggcg cacctcgtca tccagctgcc cgagctgggc cgggtggggc agcgctacga
      481 cctgaccatc gggctctcgc atcccggcgt gcgcaaggtg gtgtggctga tgggcccgcg
      541 catgctgggc ctggccgccg gccagctcaa cttcatcgtc tcgaccgtgc tggcctcggg
      601 cctgccggag ggcagcatca ccgcctacaa ctacgcattc cagctcagcc agatcccggt
      661 cggcgtgctc ggcgtgagcg tggccatcgc gctctttccc agcctgagcc gcgacgccgc
      721 cctcggccgg ctgcccgaca tccggcgaca ggtcgccggc agcctgcgca tcctggtctt
      781 cctggccgca ccgctgacgg cggcactgat cgtgctggcg gagccgatcg catcggtctt
      841 cttccagtac gggctcttcg gcgagcgctc tgccgaacgg accgccgggg ccctggtctt
      901 cttcgccatc ggcctggtcg gccatatcgt ggtgcacgtg ctgaatcgcg ccttctacgc
      961 catgcaggac acccgcacgc cggtcacctt cgccatgatc gcggtggcca tcaacctgcc
     1021 gctcatggcc tggctggttg gaccgatggg ggtgcagggg ctggccctgg cgctctccat
     1081 cagctccctc ctggaggtgg ccgccctgct gtgggcgctg cggcggcgga tcgagtcgat
     1141 cgaggacggc gaagtcgcac gctccgcggc gcgctccggg gtggcaccag gcgcggcggc
     1201 gctcctgatg cttggcgggc tgaccctggt gcaggccacg cttcccggct tgctggacca
     1261 cgggctgggc cggctggtgg cggtggcggc gctgacctcc gctggcggcg ccatctacct
     1321 ggtggtggcc tccgccctcc gcgcgccgga gatcgggcag ctgcgggcgc tgctcgcgcg
     1381 gcgacgtggg ggagcggcgg catgattcgc gtccgtcccg ccaccgatgc cgaccgagaa
     1441 gcgtggcagg cattcctgga gcgcaccccc gccggcgact tcctgcacga ctggagctgg
     1501 ggcgcggtgg ccgccttcga cgggcagccg cagcggcgct tcatggcgga ggaggacggg
     1561 acggtggtgg ccatcgtggc cgcccagttg c
//
LOCUS       DATACA010000508         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1516063, whole genome shotgun sequence.
ACCESSION   DATACA010000508 DATACA010000000
VERSION     DATACA010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516063"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..124)
                     /locus_tag="VHK28_10265"
                     /pseudo
     CDS             complement(1..124)
                     /locus_tag="VHK28_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006647790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            173..979
                     /locus_tag="VHK28_10270"
     CDS             173..979
                     /locus_tag="VHK28_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142634.1"
                     /translation="MLTSLAQASAGSASILGVSVGDGEPNRRGLVGYLDQDPRFHGWM
                     RGRELLELVGDLHGMRAPALRTRVDEVLELVGLTDAARRAIGGYSGGMRQRLGLAQAI
                     LNRPPVLLLDEPVSALDPEGRRDLLEVIRRLGGESTVLFSTHILNDVERVCDRVAILD
                     RGRLVTAGPMEELLARYARPVYVVEPEATDQSALELLAASLRDVSGVTDAQAMGTTVR
                     VSVSDDAEAGRRLLAAVATADVVVVRFERQRPTLEDVFLQLVGRAAREAA"
     gene            976..1740
                     /locus_tag="VHK28_10275"
     CDS             976..1740
                     /locus_tag="VHK28_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2142635.1"
                     /translation="MIGLRPLLGKELREQWRTRRLLVVAVVFLVLGIGSPFLARYTPE
                     LIQALGGVPFEIEFPEPTLADAASQWLRNLGQAGILTAILLSMGSVATEKERGTAALL
                     LTKPASRGSFLAAKLLAIGATLAVSLLLGSAGGYLYTLLLFEPPSVSGWTGMTALLLL
                     ALLAYAALTFLGSTLTRSPLAAAGLGVAGMVLIAIVSALPSVAPYTPAGISGQPALAL
                     AVGTDAGGLIGPVVVNAALVLVLAGLSWFAFRRQEL"
     gene            complement(1742..2122)
                     /locus_tag="VHK28_10280"
     CDS             complement(1742..2122)
                     /locus_tag="VHK28_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142636.1"
                     /translation="MGTVAAPHRSPTAPKERDWDWDGTDKLDRLREEGGWASVARAHA
                     WYDTSEDPEHDPPREKGAYKLPHHELIDGDLKVVWRGVVAAMTVVNGARGGVDIPDKD
                     RRKVYDHLAAHYGEFDEEPPDYKA"
     gene            2352..>3708
                     /locus_tag="VHK28_10285"
     CDS             2352..>3708
                     /locus_tag="VHK28_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2142637.1"
                     /translation="MRSPPTLGIQLLGRFAVVLPDGQRAGPFARPTARRLVQLLCLRP
                     DRRIGREELAEILFPELAPARAANGVAKALSMARSAIGAVGGRHVPALEADRTSIWLS
                     DQVAVQIDLDDHRAALHDALAVPPGDVRDAALTAAVANDATLLPDEPYADWAGQARDA
                     LAELRLTARLELARNRTDGHGRADRAAAIDAWNEVAARQPASEEAAIALMRAHLAARQ
                     RDRAIRAYVQCVRALQDELDVGPSPELEAAYAELVGGSRGQAAQPSVDPIPERPLFGR
                     DRVRSRVRRRLARTPAGGGSALLLVGPTGIGKSSLLGALTAELAAAGWTVMSGRSVPD
                     DRRAPYAALRQALAPVIARGGVSQLLGKALDVGTASTVTGEPEPTEGDRLRLAEEVAA
                     LLDWEAASHPLALALDDVQWADPAMLALLRRLASGCDAASQSSSAATSSARRRRSPSV
                     GS"
BASE COUNT          444 a         1366 c         1336 g          562 t
ORIGIN      
        1 cgaccagccc gcgccggttc ggctcgccgt cgcctaccga cacgcccagg atcgaggccg
       61 accccgcgct ggcctgggcc agggaggtga ggatgcgcat cgtggtggtc ttgccggctc
      121 cgtccacggc ttcctgggac ccaacggcgc cggcaagacc accacgctgc gcatcctcac
      181 ctccctggcc caggccagcg ctgggtcggc ctcgatcctg ggcgtgtcgg taggcgacgg
      241 cgagccgaac cggcgcgggc tggtcggcta cctggaccag gacccgcgct tccacggctg
      301 gatgcgcggg cgcgagctgc tcgagctggt gggcgacctg cacggcatgc gggcgccggc
      361 cctccgcacg cgggtggacg aggtgctgga gctggtcgga ctgaccgatg ccgcccggcg
      421 ggcgatcggc gggtactccg gcggcatgcg gcagcgcctg ggcctggctc aggcgatcct
      481 caaccgccca ccggtgctgc tgctcgacga gccggtcagc gccctcgatc cggagggccg
      541 ccgcgacctg ctggaggtga tccggcgcct cggcggcgag tccaccgtgc tgttcagcac
      601 ccacatcctc aatgacgtgg agcgcgtctg cgaccgggtc gcgatcctgg accgcgggcg
      661 gctggtcacc gctgggccga tggaggagct gctggcccgc tacgcccggc cggtgtacgt
      721 cgtcgagccg gaggcgaccg accagtcagc cctggaactg ctggccgcgt cgctgcgaga
      781 tgtctccggc gtgacggatg cgcaggccat gggcacgacg gtccgcgttt cggtcagcga
      841 cgatgccgag gccggcaggc ggctgctggc ggccgtggcg acggccgacg tggtcgtggt
      901 ccgcttcgag cggcagcggc cgacgctgga ggacgtcttc ctgcagctgg tcggccgcgc
      961 tgcccgggag gccgcgtgat cgggctgcgg ccgctgctcg gcaaggagct ccgcgagcag
     1021 tggcgcacca ggcgcctgct ggtggtggcc gtggtgttcc tggtgctggg catcggctcg
     1081 cccttcctgg cgcgctacac gccggagctg atccaggcgc tggggggcgt gcccttcgag
     1141 atcgagttcc cggagccgac cctcgccgac gcggccagcc agtggctgcg caacctcggc
     1201 caggcgggca tcctgaccgc catcctgctg tcgatgggca gcgtcgccac cgagaaggag
     1261 cgcgggacgg cggccctgct gctgaccaag ccggcctcgc gcggctcgtt cctggccgcc
     1321 aagctgctgg ccatcggcgc cacgctggcg gtcagcctgc tccttggttc ggccggcggg
     1381 tacctctaca cgctcctcct gttcgagcca ccgtcggtga gcggctggac gggaatgacg
     1441 gcgctgctcc tgctcgccct cctcgcctac gccgcgctca ccttcctggg cagcacgctg
     1501 acccgctcgc cactggccgc cgccgggctc ggggtggccg gcatggtcct gatcgccatc
     1561 gtctcggcgc tgccctcggt cgcgccctac acgccggccg gcatcagcgg ccagcccgcg
     1621 cttgcgctgg cggtcgggac cgatgccggc gggctcatcg gtcccgtggt cgtcaacgcg
     1681 gccctggtgc tggtgctggc cgggctctcc tggttcgcct tccgcaggca ggagctctag
     1741 ctcaggcctt gtagtccggc ggctcctcgt cgaactcgcc gtagtgggcg gccaggtggt
     1801 cgtagacctt gcggcgatcc ttgtccggga tgtccacgcc accccgcgcg ccgttgacca
     1861 cggtcatggc cgccaccacc ccgcgccaga cgaccttcag gtcaccgtcg atcagctcgt
     1921 ggtgcggaag cttgtaagcg cccttttccc gcggcgggtc atgctccggg tcctcgctcg
     1981 tgtcgtacca ggcgtgggcg cgggcgacgc tcgcccagcc accctcctcg cgcagtcgat
     2041 cgagcttgtc ggtgccgtcc caatcccagt ctcgctcctt cggagcggtg gggctgcgat
     2101 gtggcgctgc aacggttccc atgcgtgcct cctctgcttg ctgctggagg cctgcagcac
     2161 cgatgccaac ctgcgcgcgg cccctgcggt gaccgatcta tgagggcaag gagcacggga
     2221 agcggttgtt ccgcgtcatc tgactcatct atcggggcgc tgagcaccgg atggcacgcg
     2281 aacggcccgt tcctgcggtc cttgacctga tagatcggtc aggaggactc acccgctaga
     2341 ctcgcctttc catgcgctcc ccgcccacgt tgggcatcca gctgctcggc cggtttgcgg
     2401 tcgtgctgcc cgatggccag cgcgccggcc cgtttgcccg tcccacggcc cgccgcctcg
     2461 tgcagctgct ctgcctgcgg ccagaccgca ggatcggccg cgaggagctg gccgagatcc
     2521 tcttcccgga gctggctccc gcccgtgcgg ccaacggcgt ggccaaggcg ctctccatgg
     2581 cccgctcggc gatcggcgcc gtgggtggcc gtcatgtccc ggcgctcgag gcggaccgga
     2641 cctcgatctg gctctcggac caggtggccg tgcagatcga cctcgacgac catcgggcgg
     2701 cgctgcacga cgccctcgcc gtaccgcctg gcgacgtccg ggacgccgcc ctcaccgcgg
     2761 ccgtggccaa cgatgccacc ctcctccccg acgagcccta tgcggactgg gccgggcagg
     2821 ctcgcgacgc gctggcggag cttcggctca cggcgcgctt ggagctggcg cgcaaccgaa
     2881 ccgacggcca cgggcgagcc gaccgtgccg cggcgatcga tgcctggaac gaggtggcgg
     2941 cgcgccaacc tgccagcgag gaagccgcca tcgcgctgat gagggcccat ctcgccgcac
     3001 gccagcgcga tcgagccatc cgggcctacg tccaatgcgt tcgagccctg caggacgagc
     3061 tcgacgtggg cccatcgccc gagctggagg cggcctatgc agagctcgtc ggcggcagcc
     3121 gcgggcaggc ggcgcaacca tccgtcgacc caatccccga gcgaccgctg ttcggccggg
     3181 atcgcgtacg gtcccgcgtt cgccgcaggc tggctcggac tcctgcggga gggggcagcg
     3241 cgctgctgct tgtcggcccg accggcatcg ggaagagcag cctgctcggc gcgctgaccg
     3301 cggagctggc ggccgccggc tggacggtga tgagcgggcg ctccgtgccg gacgaccgcc
     3361 gcgcgccgta cgccgcgctg cgccaggcac tggcgccggt gatcgcgcgc ggcggcgtca
     3421 gccagctgct gggaaaggcg ctggacgtgg gcacggccag caccgtcacc ggcgaacccg
     3481 agccaacgga gggagaccgc ctccgcctgg cggaggaagt cgccgcgctg ctcgactggg
     3541 aggctgcgtc gcatccgctg gccctggcgc tcgacgacgt gcagtgggcc gacccggcca
     3601 tgctcgcgct gttgcggcgc ctggccagcg gatgcgacgc agcctcccag tcgagcagcg
     3661 cggcgacttc ctccgccagg cggaggcggt ctccctccgt tggctcgg
//
LOCUS       DATACA010000509         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_471634, whole genome shotgun sequence.
ACCESSION   DATACA010000509 DATACA010000000
VERSION     DATACA010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471634"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VHK28_10290"
     CDS             complement(<1..269)
                     /locus_tag="VHK28_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2142638.1"
                     /translation="MTDRFDAIVIGLGGMGSATAFHLARRGWRVVGLEQHDLLHELGS
                     SHGLTRIIRLAYHEDPSYVPLLRRAYELWHELEARAGERLLITTG"
     gene            complement(421..633)
                     /locus_tag="VHK28_10295"
     CDS             complement(421..633)
                     /locus_tag="VHK28_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142639.1"
                     /translation="MMYLSLDPLALELHAARRTELLRAQAPSDAVRVVGLRRRIGLAL
                     VGLGTRLAPEEAAVRGPRMRPAAHHH"
     gene            769..1368
                     /locus_tag="VHK28_10300"
     CDS             769..1368
                     /locus_tag="VHK28_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX2142640.1"
                     /translation="MTAHERDATAPTQDVVDRMLKIWKTELPDLDLDIEGIVERIQKL
                     NKHLDRALNETLANFQLDRGEWWLLGALRRSGPPYRRSPGHLAQEMGLSSGAMTKRLD
                     RLETAGLVRRLPDPNDRRSLQVELTEAGWQMWQDSVSVQAQKEQMVASALSREEKEQL
                     NTLLRRLMLEFERQQAASQGAAQPAASAPPSSEPAAARR"
     gene            complement(1314..1946)
                     /locus_tag="VHK28_10305"
     CDS             complement(1314..1946)
                     /locus_tag="VHK28_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase"
                     /protein_id="HEX2142641.1"
                     /translation="MTDAGGSSAPPHAGFDPELRIDWVEADELADGRPGRLGLTFLPG
                     KRGASTRYPGHVYRRDSATDLAEMRRRGIARLILLVDDDELAQWGDLRIIEIGRALGV
                     EVRRFPMADGSPPASAAEMDAILAATREARAATDVAVACMGGVGRSGLVAACALIRAG
                     RDPESAIRRVRQVRHPTAVETPAQEAFARDYAAYLRAAAGSLEGGADAAG"
     gene            complement(1946..2476)
                     /locus_tag="VHK28_10310"
     CDS             complement(1946..2476)
                     /locus_tag="VHK28_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX2142642.1"
                     /translation="MARRLLIFNAPDRFVAGVIGEPGDRTFYLQARQGNAVASVALEK
                     LQVAALAARLDDLLTAVADEAPAPTDVTADEGPLEEPVIDLFRVGAMALAWDSGGGRV
                     VIEAQPLSEDGDYVEVPDDVEEGPDVLRVAISPAHAHEFVRRASALVAAGRPTCPFCG
                     EPLEPGGHFCPKVSLN"
BASE COUNT          402 a          938 c          906 g          365 t
ORIGIN      
        1 ctgccggtgg tgatcagcag ccgctcgccg gcgcgcgcct ccagctcgtg ccacagctca
       61 taggcgcggc gcagcagcgg aacgtaggag gggtcctcgt ggtaggcgag ccggatgatc
      121 cgcgtcagcc catgcgacga gccgagctca tggaggaggt cgtgttgctc caggccgacg
      181 acgcgccagc cacgccgggc gagatggaag gcggtggcgc tccccatgcc gcccaggccg
      241 atcacgatgg cgtcgaatcg gtccgtcacg ccggccagtg tacgagcgcc gggcaatgag
      301 aagggcgccc ggtggtgcct accggacgcc catctcgtcg gcccatcacc tccgctgctc
      361 gggcggcgga gacgggcccc gcgcgaccgg cccaatcatc ggccgcgcgt gtgccagggg
      421 tcagtggtgg tgcgctgccg ggcgcatccg cggtccgcgg acggcggcct cctccggggc
      481 cagccgcgtc ccgaggccga ccagggccag cccgatcctg cggcgcagcc cgaccacgcg
      541 gaccgcgtcg gacggcgcct gcgctcgcag cagctcggtc cgtcgagcag cgtgcagctc
      601 gagcgccaag gggtcgagtg agaggtacat caagggatct ccggggtctc gcgccccgac
      661 gacggtggcg cggcttcatt acaagattct ttacgtcgaa cgatcggacg agaaggtacc
      721 acgcaggatg tcttgacgtc aagaggtttt acgtggagaa tcgcgagcat gaccgcccac
      781 gaacgcgacg cgactgctcc tacccaggac gtggtggacc gcatgctcaa gatctggaag
      841 acggagctgc cggacctcga cctggacatc gagggcatcg tcgagcgcat tcagaagctg
      901 aacaagcacc tggaccgcgc cctcaacgag acgctggcga acttccagct cgatcgcggt
      961 gagtggtggc tgctcggcgc gcttcgccgc tcgggaccgc cgtaccgccg ctcaccggga
     1021 cacctggccc aggagatggg gctctcgtcc ggagccatga ccaagcgttt ggatcggctg
     1081 gagacggctg gtcttgtgcg gcggctcccc gaccctaacg accggcggtc gctgcaggtc
     1141 gagctcaccg aggccggctg gcagatgtgg caggactccg tatcggtcca ggcgcagaag
     1201 gagcagatgg tcgcctcggc actgtcgcgc gaggagaagg aacagctcaa cacgctcctt
     1261 cgccgcctga tgctcgagtt cgagcggcag caggccgctt cccagggcgc cgctcagccg
     1321 gctgcgtcag ctccgccctc cagcgagccg gccgccgccc gaaggtaggc ggcgtagtcc
     1381 cgggcgaacg cctcctgcgc gggcgtctcg accgcggtcg ggtggcgcac ctggcgcacg
     1441 cgccggatgg ccgattcggg atcgcgcccc gcacggatca gggcgcaggc agccaccagt
     1501 ccgctgcgtc ccaccccgcc catacaggca accgcgacat cagttgcagc gcgagcctcc
     1561 cgcgtggcgg ccaggatggc atccatctcc gccgcggagg ccggtggcga cccgtccgcc
     1621 atcggaaagc ggcggacctc cacccccagc gcgcgtccga tctcgatgat gcgcaggtcg
     1681 ccccactgcg ccagctcgtc gtcatccacc agcaggatca gccgtgcgat gccgcgcctg
     1741 cgcatctcgg ccaggtccgt ggcgctgtcg cggcggtaga cgtgccccgg gtagcgggtc
     1801 gaggcgccgc gcttgccggg caggaacgtc aggcccagcc gtcccggccg gccgtcggcc
     1861 agctcgtccg cctccaccca gtcgatgcgc agctccgggt caaatcccgc gtgcggcgga
     1921 gcggaagagc cgcccgcatc ggtcatcagt tcaggctgac cttggggcag aaatggccgc
     1981 caggttcgag cggctccccg cagaacggac aggtcgggcg gccggcggcc accagcgcgc
     2041 tggcgcggcg gacgaactcg tgggcatggg ccggagagat cgccacccgc agtacgtccg
     2101 gtccttcctc cacgtcgtcc gggacctcca cgtagtcgcc gtcctcgctg agcggctggg
     2161 cctcgatgac caccctgcca ccgcccgaat cccaggccag cgccatggca cccacccgga
     2221 acaggtcgat caccggctcc tccagcggcc cctcgtcggc tgtcacgtcg gttggtgccg
     2281 gcgcctcgtc tgccacggcg gtcagcaggt catccaggcg tgcggccagg gccgcgacct
     2341 gcagcttttc gagcgccacg ctggccacgg cgttgccctg ccgggcctgc aggtagaagg
     2401 tccggtcgcc cggctcaccg atgacgccgg ccacgaatcg gtcaggggca ttgaagatca
     2461 ggagtcgacg cgccatatcg gcaatcgtag cccggcacgg ccggtagagt gtggcgcgac
     2521 atggtgcgct cccgctccca tgcgcgtcga ctcctgatct tcaatgcccc tgaccgattc
     2581 gtggccggcg ccatcggtga gccgggcgac c
//
LOCUS       DATACA010000510         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2882621, whole genome shotgun sequence.
ACCESSION   DATACA010000510 DATACA010000000
VERSION     DATACA010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882621"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VHK28_10315"
     CDS             complement(<1..1005)
                     /locus_tag="VHK28_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX2142643.1"
                     /translation="MDGQPLIEVDNLKVYFPILTGVFQRRSGEVKAVDDISFEIRKGE
                     TLGLVGESGCGKSTTGRALIRLREATGGTVKFDGVDLTSVSTGDLRRMRRRMQIIFQD
                     PYSSLNPRMTVGSIISEPVETHNLASGKAKDERVRELLRLVGLNPNYTNRYPHEFSGG
                     QRQRIGVARALAVEPEFIVCDEPISALDVSIQAQVLNLLVELREQFGLTYLFIAHDLS
                     VVRHISDRVGVMYLGKLAELGPPNAIYDTPAHPYTRALLSAVPIPDPKAERRRKRVIL
                     TGDVPSPANPPSGCRFHTRCWLYEQLGRPENCRTEDPPLRTVGSDHRAACHYAEEALK
                     S"
     gene            complement(1023..2033)
                     /locus_tag="VHK28_10320"
     CDS             complement(1023..2033)
                     /locus_tag="VHK28_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142644.1"
                     /translation="MADSRRHARKLLEVKNLSTHFFTADGVVHAVDNVSFELDYGETL
                     GLVGESGCGKSVTALSLTRLVPNPPGKIVSGDILFDGVNVMKLSNEEMRLLRGKRIGF
                     IFQDPMTSLNPTLTIGYQIAESARIHMGLSRKAADDRAAELLSKVGIPRARDRMSDYP
                     HQFSGGMRQRVMIAIALACDPMLLLADEPTTALDVTIQAQILELIRSLSSEFGTAVIL
                     ITHDLGIAAGMCDRVNVMYAGRIVETGGVDDIFEQPRMPYTWGLMDSLPRLDEVKGER
                     LRTIEGLPPDLIDPANECRFSPRCPYARDICREQEPELTPRAEERHLARCHGTEPGGW
                     IQ"
     gene            complement(2143..3096)
                     /locus_tag="VHK28_10325"
     CDS             complement(2143..3096)
                     /locus_tag="VHK28_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142645.1"
                     /translation="MASTTTALEAPVARRQSQGLWRDAFGRLIKNRLAIVGGLLVLLL
                     AFIAIAGPFISGTWLHDFRDQNVALTAQYGGRPVPPLSEPGYILGTDKLGRDLLARIF
                     DGAQISMTVALVVQAVVLLIGIPVGAIAGWFGGRIDNYLMRFTDVVYAFPDLLFIIIL
                     TMAFKETWFGRAMDGLLLVFVAIGIVSWITMARLVRGQLLSLKETEFVEAARAIGVPD
                     RRIVTRHLLPNAVGPVIVAVTLGIPGAILAEATLAFIGIGAQAPRASWGSLIDDGRDF
                     IRGDPHLVLYPALAVAIALLSFTFLGDGLRDALDPKLKGKQ"
     gene            complement(3107..4054)
                     /locus_tag="VHK28_10330"
     CDS             complement(3107..4054)
                     /locus_tag="VHK28_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142646.1"
                     /translation="MVGYIVRRVLWIIPVLLVVSIITFTLMKTTPGGPWDREKELSPR
                     QIAVLNAKYGLDDPFVVQYIRWLVGWPQEDGFQRGLLLGDLGPSYRYPDRSVNDIIAD
                     GIWTTVQLGVMAFLLAVLVGIPLGVIAALRQNRIPDYVATFISVLGITTPSFVAATLL
                     MVVFSVGLGWFPTSGWRGPEYWVLPTLALALAPIASIARYTRASMLEVTRKDYVRTAH
                     SKGLKERSVVVIHMIRNALIPVVTILGPILAFLVTGSFIIELLFSIPGVGKFYVTAIS
                     QRDYGLIMAMTMLYAGAVAIMNMIVDVLYAYIDPRIRYN"
     gene            complement(4208..>4735)
                     /locus_tag="VHK28_10335"
     CDS             complement(4208..>4735)
                     /locus_tag="VHK28_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2142647.1"
                     /translation="SDLGLSDASELTLQLGYNTEADHEPTVEFLQEQWRTAFGVEVEL
                     QGLEWSSYLTRLNEDPFDIFRLGWGADYPHPNNFLTDLFSCTSGNNNMQYCNEDYDAL
                     LAEAAVTPELDAQVELYNQAQEILVEEAPVIFISWGGRFTLVKPWVESLQITPQDFSS
                     GAYFYTSVTIGARDE"
BASE COUNT          782 a         1547 c         1608 g          798 t
ORIGIN      
        1 gctcttgagc gcctcctccg cgtagtggca ggccgcacga tggtcactgc ccacggtgcg
       61 caacggcggg tcctcggtac ggcagttctc cggccgcccg agctgctcgt acagccagca
      121 gcgagtgtgg aagcggcagc ccgagggcgg gttggccggt gacggaacgt cgccggtgag
      181 gatgacgcgc ttgcgacgcc gctcggcctt cgggtcgggg atgggcaccg ccgacagcag
      241 cgctcgggtg tacggatggg ccggcgtgtc gtagatggcg ttgggcgggc ccagctcggc
      301 gagcttgccc aggtacatca cgccgacccg gtcgctgatg tggcgcacca cgctcaggtc
      361 gtgggcgatg aagaggtacg tgagcccgaa ctgctcgcgc agctcgacca gcaggttgag
      421 tacctgggcc tggatgctga cgtccagggc gctgatcggc tcgtcgcaga cgatgaactc
      481 cggctccacc gcgagcgccc gggccacccc gatgcgttgc cgctggccgc cggagaactc
      541 gtgcgggtat cggttggtgt agttggggtt gaggcccacc aggcgcagca gctcgcggac
      601 ccgctcgtcc ttggccttgc cggacgccag gttatgggtc tcgacgggct ccgagatgat
      661 ggatccaacc gtcatccgcg ggttcaggct ggagtagggg tcctggaaga tgatctgcat
      721 ccgacgccgc atgcggcgca ggtcgccggt cgagacgctg gtgaggtcca cgccgtcgaa
      781 cttcaccgtg ccgcccgtcg cctcgcgcag tcggataagg gcgcgaccgg tagtgctctt
      841 gccgcagccg gactcgccca ccaggcccag ggtctcgccc ttgcggatct cgaagctgat
      901 gtcgtcgacg gccttgacct ctccggagcg gcgctggaag acgccggtca ggatcgggaa
      961 gtagaccttc aggttatcga cctcgatcag aggctggccg tccatgctgc cctgcccgtt
     1021 ggtcactgga tccagcctcc tggctcggtg ccgtggcagc gagcgaggtg acgctcttcg
     1081 gcacgcgggg tgagctccgg ctcctgctcg cggcagatgt cgcgggcgta cggacagcgc
     1141 ggagagaatc ggcattcgtt ggccggatcg atcaggtccg ggggcagccc ctcgatggtt
     1201 cgcagccgct cgcccttgac ctcgtcaaga cgcggcagcg agtccatcag gccccaggtg
     1261 tagggcatac gcggctgctc gaagatgtcg tccacgccgc cggtctccac gattcggccg
     1321 gcgtacatga cgttgacccg gtcgcacatt cccgccgcga tgccgaggtc gtgggtgatc
     1381 aggatcaccg ccgttccgaa ctcggatgac agtgagcgga tgagctccag gatctgagcc
     1441 tggatcgtca catccagcgc ggtggtcggc tcatcggcca gcagcagcat cgggtcgcag
     1501 gccagggcaa tggcgatcat cacccgctgc cgcatgccgc ccgagaattg gtgcgggtag
     1561 tcgctcatgc ggtcgcgcgc ccgcgggatg ccgaccttgc tgagcagctc cgcggcccga
     1621 tcgtctgccg ccttgcgtga caggcccatg tggatgcggg ctgactcggc gatctggtag
     1681 ccgatggtca gcgtggggtt caggctggtc attgggtcct ggaagatgaa gccgatgcgc
     1741 ttcccgcgca gcagccgcat ctcctcgttc gacagcttca tgacgttgac gccgtcgaag
     1801 aggatgtcgc cgctgacgat cttgccgggc gggttgggga ccagccgggt cagcgacagg
     1861 gcggtgacgc tcttgccaca gcccgactcg ccgaccaggc cgagcgtctc gccgtagtcg
     1921 agctcgaagc tgacgttgtc gacggcgtgg accacgccat ctgcggtgaa gaagtgggtc
     1981 gacaggttct tgacctcgag cagcttgcgc gcgtgacgtc gcgagtccgc cagctgcgcg
     2041 gcgaggcgcc gaccctcttc ggtttctacg tggatgtctt cgggcagctc ggtcacagcc
     2101 gggctgcccg gtgcccggcc ctggtcgtct gcgacggtca aattactgct tccccttcag
     2161 cttcggatcc agtgcgtctc gcaggccatc ccccaggaac gtgaagctga ggagggctat
     2221 ggccaccgcc agtgccggat acagcaccag gtgtgggtcc ccccgaatga agtcgcgtcc
     2281 gtcgtcgatc aggctccccc acgacgcgcg cggggcctgg gcgccgatgc cgatgaaggc
     2341 cagcgtggcc tcggccagga tggcgcccgg gatgccgagc gtcaccgcca cgatgaccgg
     2401 tccgacggca ttcggcagca ggtgccgcgt gacgatgcgg cggtcgggca cgccgatggc
     2461 ccgtgccgcc tccacgaact cggtctcctt caggctgagc agctgcccgc gcaccaggcg
     2521 agccatggtg atccaggaca cgatgccgat ggccacgaac acgagcagca acccgtccat
     2581 tgcccgtccg aaccaggtct ccttgaaggc catggtcagg atgatgatga acagcaggtc
     2641 cgggaaggcg tacaccacgt ccgtgaagcg catcagatag ttgtcgatgc ggccgccgaa
     2701 ccagccggcg atggcgccga ccgggatgcc gatcagcagg acgacggcct gcaccaccaa
     2761 cgccacggtc atgctgatct gcgctccgtc gaagatgcgc gccagcagat cacggcccag
     2821 cttgtccgtc cccaggatgt agccgggctc cgacagcggc ggcaccggcc ggccgccgta
     2881 ctgggcggtc aacgccacgt tctggtcacg gaagtcgtgc agccaggtac cggagatgaa
     2941 cgggccggcg atggcgatga acgcgagcaa cagcacgagg agcccgccca cgattgccag
     3001 ccggttcttg atcaggcgcc cgaaggcgtc gcgccacagg ccctggctct gccgtcgagc
     3061 gactggagcc tcgagggcgg tggttgtgga tgccatctcg agcctcctag ttgtaccgga
     3121 tccgggggtc gatgtacgcg tacagcacgt ccacgatcat gttcatgatc gcgaccgctc
     3181 cggcgtacag catcgtcatg gccatgatca gcccgtagtc gcgctggctg atggccgtga
     3241 cgtagaactt cccgactccg gggatgctga acagcagctc gatgatgaac gatccggtga
     3301 ccaggaaggc caggatcggc cccaggatgg tgaccaccgg aatcagcgcg ttgcggatca
     3361 tgtggatgac caccacgctg cgctccttga gccccttgct gtgagcggtg cgcacgtaat
     3421 ccttgcgggt cacctccagc atgctggcgc gggtgtagcg cgcgatcgag gcgatgggcg
     3481 ccagcgccag ggccagtgtg ggcaggaccc agtactcagg cccgcgccat ccgctggtcg
     3541 ggaaccaccc caacccgacg ctgaagacca ccatcagcag cgtggccgcc acgaagctgg
     3601 gcgtggtgat gcccagcacg ctgatgaagg tggccacgta gtccggtatg cggttctgtc
     3661 tgagcgcggc gatcaccccc agcgggatgc ccaccagcac ggccagcagg aaggccatga
     3721 cgcccagctg cacggtggtc cagattccgt cggcaatgat gtcgttgacg ctgcggtccg
     3781 ggtaccgata cgacggcccc aggtcgccga gcagcagccc gcgctggaat ccgtcctcct
     3841 gtggccaccc gaccagccag cggatgtact gcaccacgaa cgggtcgtcc aggccatact
     3901 tggcgttcag gaccgcgatc tggcgcggcg agagctcctt ctcccgatcc cacggccctc
     3961 cgggcgtggt cttcatcagg gtgaaggtga tgatcgagac gacgaggagc accgggatga
     4021 tccagagcac ccgtcgcacg atgtagccga ccacggcttc agcctcctta agctgctggc
     4081 tcgtcttgcc gacggccccg aaccccgggc ggcgctgcct cccgagatgc ggggccgtcg
     4141 ggtggaccgg cggaccggtc cacccgagcg gggcctatct caagttgtga gcccctaccc
     4201 tactcgatta ctcgtcccgc gcaccgatgg tgacggacgt gtagaagtag gcacccgagc
     4261 tgaagtcctg cggagtgatc tgcaggctct caacccatgg cttgaccagc gtgaaccggc
     4321 cgccccagct gatgaagatc accggggcct cttcgaccag gatctcctga gcctggttgt
     4381 agagctcgac ctgtgcatcc agctcaggcg taacagcagc ctcggcgagg agcgcgtcgt
     4441 agtcctcgtt gcagtactgc atgttgttgt tgcccgaggt gcagctgaac aggtcagtca
     4501 ggaagttgtt cgggtgcggg tagtccgcgc cccagcccag ccggaagatg tcgaacggat
     4561 cttcgttcag ccgggtcagg tagctggacc actcgaggcc ctgcagctca acttccacgc
     4621 cgaaggcggt ccgccactgc tcctggagga actccaccgt cggctcgtga tcggcctcgg
     4681 tgttgtagcc cagctgcagt gtcagctcgc tggcatccga caggccgaga tcgga
//
LOCUS       DATACA010000511         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2239390, whole genome shotgun sequence.
ACCESSION   DATACA010000511 DATACA010000000
VERSION     DATACA010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239390"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VHK28_10340"
     CDS             complement(<1..397)
                     /locus_tag="VHK28_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142648.1"
                     /translation="MGWLVLLAFGVLLVACSTAHSGSPRSDATDRERTSPTVPPSATP
                     RPTPTPTALPVPAAGTYVALGDSLAVGVGAEQPDEGGYVARLFAELSALGRDGVQAAR
                     LTNVAVSGETSDSIIRDGQLARGARAIGEA"
     gene            complement(430..747)
                     /locus_tag="VHK28_10345"
     CDS             complement(430..747)
                     /locus_tag="VHK28_10345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142649.1"
                     /translation="MTEPQIAVVDNPDRERYEVTVDGELAGYLEYRDRGGRRILVHTQ
                     VDRAHAGRGLGGRLAKAALDDVTERGMGAVARCPFVRAWVGRHPEYAAIARVRPEAAE
                     GDA"
     gene            complement(744..1385)
                     /locus_tag="VHK28_10350"
     CDS             complement(744..1385)
                     /locus_tag="VHK28_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142650.1"
                     /translation="MAIGWRRAALLLIGLAGMAVLGLSFVDGWIVHARHIGGHGLTVL
                     ETVINAWERRSMPVLSAGVLLAATTVALALWRLTRADRSAGTTGLLLIGSVAALGLMI
                     GSAVPLEHNGVVVRVERSAGWPLLVSAGLVGVMTALAALLLAPRPTVLVGACLLAALL
                     VVAGTGGRALGLALAEGGNHHYADGSYWWQTIVDQCANGERAADRTTGIWVRQ"
     gene            1568..2038
                     /locus_tag="VHK28_10355"
     CDS             1568..2038
                     /locus_tag="VHK28_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX2142651.1"
                     /translation="MGRAIAETLVAEGLLAELHPVEVLGALGEFDVAVIGSGVYVGRW
                     LEPARRFVERHAEQLAAMPVWLFSSGPLGEKDPQPAGDPTDVAALVNQVRPRAHRVFP
                     GALRREGLGLMERGVVRMVGAPYGDFRDTGAIHAWAHEIAAELRQPVGAVPGRR"
     gene            2055..3215
                     /locus_tag="VHK28_10360"
     CDS             2055..3215
                     /locus_tag="VHK28_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2142652.1"
                     /translation="MRGTTSRLDGAPRTTWHVAGVLAATAGLAASVYLLVEYLTGQSG
                     ICLTGSGCDSVRGSAYAYPLGIPMPAFGVAYFSLAAWLSVSSVDGTPLRGVERRHALL
                     VLAGIGALGFAVLTAIEAFIIGAFCSWCLVAAFAGWTLLVAAVGAWREGRGSDQGDEG
                     RSSRARQHRAQAIAAARSRVRRALGMSGAASGLLVVSLLLLGMQQAPAPSAADSLAPE
                     GSPRLGSGAVTLVEFSDFQCPACATVSPVLHQLAANDEVTLVYRHFPLESIHANALGA
                     ARAAAAAQRQQAFWPMAEALFARQAEWQALGTAQADTYFVALAGQLGLDQTQWGTDYA
                     SDAVRDQVAADARAASELRLRGTPSVFIDGEPHEGPMSLDALRAAIADARGG"
     gene            3288..>4523
                     /locus_tag="VHK28_10365"
     CDS             3288..>4523
                     /locus_tag="VHK28_10365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX2142653.1"
                     /translation="MRPTLPYPRGLIAPRRRQVRPATSIRRRRRHRSWLARDIYLAYT
                     AALPPIAALVLLASQQHLQLLALGLLSLIFVGAQAVLGTLSPRRRPLGPLAWSSLRLA
                     VSLLFVAGIVELGGGASGPMVALFLPVVVAAAAIGTVQGVMVGALASVFYLAPEFSDM
                     RAPGQLALGGITLAGVTVLLALGTRRLVVAVERTSRQLRSAMRSERRRSRQIAGMEEV
                     SRLLVAAGPTPDTLDSVLAVLVDRFGYNHVSIYLAEGEVLVLGAQRGYEQAISQFDGS
                     AGVVGRAMRSRRVQFVPDVARDPDYISVFDEVRSEICAPLLVGGQLIGILNVEARDDL
                     DHTDRDLVATLADRVATMVALGRDRQALAERAAVFRSLHEFTQAVSGTLDRDRLAVAM
                     VDAARRVVPADVVVLTVLEQ"
BASE COUNT          570 a         1639 c         1635 g          679 t
ORIGIN      
        1 cagcctcgcc gatggcgcgc gcgccgcgcg caagctgccc gtcccggatg atggagtcgc
       61 tcgtctcgcc ggagacggcc acgttggtga gccgggcggc ctgcacgccg tcccttccca
      121 gtgcggaaag ctccgcgaag aggcgcgcca cgtaaccgcc ctcgtcgggc tgctcggcac
      181 ccacgccgac ggccagcgag tcgccgagcg cgacgtacgt gccggcggcc ggcactggca
      241 gcgccgtggg cgtgggagtg gggcgcggag tggcgctggg cggcacggtg ggtgatgttc
      301 gctcacggtc agtggcatcg gagcgcggcg agccggaatg tgcggtcgag caggcgacca
      361 gcaggacgcc gaaggcaagg agcacgagcc agcccacgcc gacacgcggc gcacgaacga
      421 tgccagcgat caggcgtcgc cctcggctgc ctccggcctc acccgcgcga tcgcggcgta
      481 ctccgggtgg cgccctaccc acgcgcgcac gaacgggcag cgcgccacgg cgcccatgcc
      541 gcgctcggtg acgtcgtcca gcgccgcctt ggccaagcgc ccccccaggc cacggccggc
      601 gtgcgcccgg tccacctggg tgtggaccag gatccgccgc ccgccgcggt cccggtactc
      661 caggtagccg gccagctcgc cgtccaccgt cacctcgtag cgctcgcgat cggggttgtc
      721 gacgacggcg atctgcggct cggtcattgg cgcacccaga tccccgtggt ccggtcggct
      781 gcccgctcgc cgttcgcgca ctggtccacg atcgtctgcc accagtacga gccgtcggcg
      841 tagtggtggt tgcctccctc ggccagcgcc aggccaagtg cccgcccgcc ggttccggcc
      901 accaccagga gcgcggccag caggcaggcg cccaccagca ccgttggtcg cggggccagc
      961 agcagcgcgg ccagggccgt catgaccccc accaggccgg cggagaccag cagcggccat
     1021 ccggcgctcc gctccacccg gaccacgacc ccgttgtgct ccagcgggac ggcagagccg
     1081 atcatcagtc ccagcgcggc gacgctaccg atcagcagca ggccggtcgt ccctgcggac
     1141 cgatcggcgc gggtgagcct ccacagcgcc agggcgacag tggtggcggc gagcagcact
     1201 ccggcgctca ggacaggcat cgagcggcgc tcccaggcgt tgatgaccgt ctccagcacg
     1261 gtcaggccgt gcccgccaat gtgccgcgcg tgcacgatcc agccgtccac gaacgacagc
     1321 cccagcacgg ccatcccggc cagaccgatg agcagcagcg cggcacggcg ccatccgatt
     1381 gccacggctg gattctatcg ggggccagag gtcaggcgca gacgggccgc ctggcactcc
     1441 aaccgggcgt ggcgggttgc ctacgctcga cgccacgggg cgtatgcccg gctgtgcccg
     1501 ccccagattg gagtgttcga tgcgcgttct cgttgccgtg gcttccaggc acggctaaac
     1561 cctggagatc ggacgggcca tcgccgaaac gctggtggcg gaagggctgc tcgccgagct
     1621 gcatcccgtc gaggtgctgg gggccctcgg tgagttcgat gtcgccgtca tcggcagcgg
     1681 cgtctacgtc gggcgctggc tggagccggc ccggcgcttc gtggagcgtc atgccgagca
     1741 gctggccgcc atgccggtat ggctcttctc gagcgggccg ctgggcgaga aggacccgca
     1801 gccggccggc gacccgaccg acgttgccgc gctggtgaac caggttcgac cgcgtgccca
     1861 ccgcgtcttt cccggagcgc tgcggcggga ggggctcggc ctcatggagc gtggggtagt
     1921 gcgcatggtc ggcgccccgt acggcgattt tcgcgacacc ggcgccatcc atgcctgggc
     1981 gcacgagatt gccgccgagc tgcgtcagcc agtcggagcc gtgccaggtc gccgctgaca
     2041 cccggtcggg gccgatgcgt ggtaccactt cccggctgga tggcgcgccg cgcaccacgt
     2101 ggcacgtcgc gggagtgctc gcagccacgg ccggcctggc agcctctgtc tacctgctgg
     2161 tcgagtacct gaccgggcag tcggggatct gcctcaccgg ttcgggctgc gacagcgtcc
     2221 gtggcagcgc ctacgcctac ccgctcggca tcccgatgcc ggcattcggg gtcgcgtact
     2281 tttcgctggc cgcgtggctg tcggtgagca gcgtggatgg cactccgctc cggggcgtcg
     2341 agcggcgcca cgccctgctg gtgcttgcgg gtatcggcgc cctggggttc gcggtgctga
     2401 ccgcgatcga ggccttcatt atcggcgcgt tctgcagctg gtgcctggtg gccgcgttcg
     2461 ccgggtggac cctgctcgtc gccgcggtcg gtgcctggcg tgagggacgc ggcagcgacc
     2521 agggtgatga agggcgctcc agccgggcca ggcagcatcg ggcgcaggcc atcgccgccg
     2581 cgcgctctcg cgttcgccgc gcgcttggga tgagcggcgc ggccagcggc ctgctggtcg
     2641 tctcgctgct tctgctcggc atgcagcagg cgccagcacc ctccgccgcc gactcgctgg
     2701 caccggaggg cagcccgcgg ctggggagcg gagcggtgac cctggtcgag ttctcggact
     2761 tccagtgccc ggcctgcgcg acggtcagcc cggtgctgca ccagctggca gccaacgacg
     2821 aggtcacgct ggtgtaccgc cactttccgc tggagagcat ccacgccaac gcgctcggcg
     2881 ccgcccgcgc tgcggcggcc gcccagcggc agcaggcctt ctggcccatg gcagaggcgc
     2941 tgtttgcgcg ccaggcggag tggcaggcgc tgggcaccgc ccaggcggat acctacttcg
     3001 tcgcgctggc gggccagctc ggcctcgacc agacgcaatg ggggaccgac tacgcctcgg
     3061 atgccgtgcg cgaccaggtc gccgcggatg cccgcgcggc atccgagctg cgcctgcgcg
     3121 gaacgccatc ggtcttcatc gatggggagc cccacgaggg gccgatgtcg ctcgacgctc
     3181 tgcgtgccgc gatcgcagat gcgcggggcg gctgagggga catccgccgt acgtcggtac
     3241 tatcggggga tgggtgtggt ctgggtcggt tggctaccgt ccaaccgatg cggcccaccc
     3301 tgccttaccc ccgcggcctg atcgcgcccc gccggcgcca agtacgtcct gcgaccagca
     3361 tccgccgccg gcgtcgtcat cggtcctggc tggcacgcga catctacctg gcctacaccg
     3421 cggccttgcc gccgattgcc gccctggtcc tgctggcctc gcagcagcac ctccagctgc
     3481 tggcgctcgg cctgctgtcg ctgatcttcg taggcgccca ggcggtgctc ggaacgctct
     3541 cgccccggcg ccgtccgctt gggccgctgg cctggtcgtc gctgcgcctg gcggtctcgc
     3601 tgctattcgt ggctggcatc gtggaactcg gcggcggggc ctccgggccg atggtggcgc
     3661 tcttcctgcc ggtcgtggtg gccgcggcag caatcgggac cgtccagggt gtcatggtcg
     3721 gcgcgctggc atcggtcttc tacctggcac ccgagttcag cgacatgcgc gctcccgggc
     3781 aactggcgct gggaggcatc acgctggccg gcgtcaccgt gctgctggcc ttgggcaccc
     3841 gccgcctggt ggttgccgtg gagcgcacaa gccgccagct gcggtcggcc atgcgctccg
     3901 agcgcaggcg gtcgcgccag atcgccggga tggaggaggt gagccgcctg ctggtggccg
     3961 cagggccgac ccccgacacc ctcgacagcg tcctggccgt cctcgtcgac cgcttcggct
     4021 acaaccacgt ttccatttac ctggccgagg gcgaggtgct ggtgctcggt gcgcagcgag
     4081 gctacgagca ggccatctcg cagttcgacg gcagcgccgg cgtggtcggt cgcgccatgc
     4141 gcagccgccg ggtccagttc gtgcccgacg tggcccgcga tcccgactac atcagcgtct
     4201 tcgacgaggt gcgcagtgag atctgcgccc cgctactggt cggtggccag ctgatcggca
     4261 tcctcaacgt cgaggcccgc gacgatctgg accacaccga tcgcgacctg gtggccacgc
     4321 tggccgaccg ggtggccacc atggtggccc tcggtcgtga tcgacaggca ttggccgagc
     4381 gtgcggccgt cttccgcagc ctgcacgagt tcacccaggc ggtcagtggc acgctggacc
     4441 gtgaccggct ggcggtggcc atggtggacg ccgcacgtcg ggtggtgccc gccgacgtcg
     4501 tggtgctcac cgtgctggag cag
//
LOCUS       DATACA010000512         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2239484, whole genome shotgun sequence.
ACCESSION   DATACA010000512 DATACA010000000
VERSION     DATACA010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2239484"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="VHK28_10370"
     CDS             <1..1659
                     /locus_tag="VHK28_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2142654.1"
                     /translation="VGGRYRRDGVLRHLVDLQYQRNDAALARSRFRVRGDVLEFAPVS
                     GDYIVRVVFFGDEVERIVEVDELTGEVLAERREVNIYPATHYVTPRDKLLLAIDDIEA
                     EMEERVGQLAAEGRELEAARLRQRTMFDLEMMREVGFCSGIENYSRHLARREEGSRPW
                     TLIDYFPTDYLLVVDESHISIPQVHGMYKNDRTRKEIMVEFGFRLPSALDNRPLTFEE
                     FEDHMNQVVYMSATPGPYETERAQRVAEQFIRPTGVVDPSITVRPTAGQIDDLLAEIR
                     DTVARGERVLVTTLTKKMAEDLADYLAELGIKVQWLHSEVDTLERVEILRDLRLGVYD
                     VLVGINLLREGLDLPEVTLVAILDADKEGFLRSGGSLIQVIGRAARNIGGRVIMYADR
                     VTDSMRAAIEETDRRREKQIAYNAEHGITPETIIKEIRDIHEGLRRVAEERAELEVAG
                     KEPEELAAMMGRLEAQMKEAARNLEFERAAALRDEILQLRRLREELGAAPGTIPADVY
                     AAAATDGAGDGRRGSEASRVFSTATTPNVVPAGTRRRPPRGRRP"
BASE COUNT          284 a          585 c          660 g          243 t
ORIGIN      
        1 gtcggaggcc gataccgtcg cgacggcgtg ctgcgccacc tggtcgacct gcagtaccag
       61 cgcaacgatg cggcgctggc gcgctcgcgg ttccgcgtgc gcggcgacgt cctggagttc
      121 gcaccggtct ccggcgacta catcgtgcgg gtcgtcttct tcggcgacga ggtggagcgc
      181 atcgtggagg tggacgagct gaccggcgag gtgctggccg agcgacgcga ggtgaacatt
      241 tatccggcca cccactacgt cacgccgcgg gacaagctgc tgctggccat cgacgacatc
      301 gaggccgaga tggaggagcg cgtcggtcag ctggcggccg agggccgcga gctggaggcc
      361 gcccggctac gccagcggac catgttcgac ctggagatga tgcgggaggt cggcttctgt
      421 tcggggatcg agaactactc gcggcacctg gcgcgccgcg aggagggttc gcggccctgg
      481 acgctgatcg actacttccc gaccgactac ctgctggtcg tcgacgagag ccacatctcg
      541 atcccgcagg tgcacggcat gtacaagaac gaccggacgc gtaaggagat catggtcgag
      601 ttcggcttcc ggctgccatc cgccctggac aaccgcccgt tgaccttcga ggagttcgag
      661 gaccacatga accaggtggt ctacatgtcg gcgacgcccg ggccgtacga gacggagcgc
      721 gcccagcggg tggcggagca gttcatccgt cccaccggcg tggtggatcc gtccatcacg
      781 gtgcgtccca ccgccggcca gatcgacgac ctgctggccg agatccgcga cacggtggcg
      841 cgcggcgagc gggtgctggt caccacgctc accaagaaga tggccgagga cctggcggac
      901 tacctcgccg agctgggcat caaagtccag tggctgcaca gcgaggtcga caccctggaa
      961 cgggtcgaga tcctgcgcga cctgcgcctg ggcgtctacg acgtgctggt cggcatcaac
     1021 ctgctgcgcg agggcctcga cctgcccgag gtgaccctgg tcgccatcct ggacgccgac
     1081 aaggagggct tcctgcgcag cggcgggtcg ctgatccagg tcatcggccg tgcggcgcgc
     1141 aacatcggcg gccgggtgat catgtacgcc gaccgggtga ccgattcgat gcgggcggcc
     1201 atcgaggaga ccgatcgccg ccgggagaag cagatcgcct acaacgccga gcacggcatc
     1261 acgccggaga ccatcatcaa ggagatccgg gacatccacg aggggctgcg ccgcgtggcc
     1321 gaggagcgcg ccgagctgga ggtcgccggc aaggagccgg aggagctggc ggccatgatg
     1381 ggccgcctgg aggcgcagat gaaggaggcg gcccgcaacc tggagttcga gcgtgctgcc
     1441 gcgctgcgcg acgagatcct ccagctgcgc cgcctgcgcg aggagctggg cgccgcgccg
     1501 gggacgatcc cggccgacgt ctatgcggcc gccgccacgg atggtgcggg cgacgggcgg
     1561 cgggggagcg aggccagccg ggtgttcagc accgcgacca cgcccaacgt cgtcccggcc
     1621 gggacccggc gcaggccgcc gcgcggtcgc cggccctagc ggaggccgga cctcgtggcc
     1681 gggacgatgc cgcatgcgat cttcgtggag gagaacgcgg atggggctgg cggcttcgcc
     1741 gcctacgcgg ccgagcttcc gggctgcgcc gt
//
LOCUS       DATACA010000513         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2079853, whole genome shotgun sequence.
ACCESSION   DATACA010000513 DATACA010000000
VERSION     DATACA010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079853"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VHK28_10375"
     CDS             complement(<1..474)
                     /locus_tag="VHK28_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PA-phosphatase"
                     /protein_id="HEX2142655.1"
                     /translation="MKTLARPMTAIIAVVIAFSGLVAGAPARLPLAAAAEGNAVTDWS
                     LIAQNAWSVGRPGTSGMYVAALVHAAIYDAVVAIDGGGEPFISSPTGDGANPDAAVAA
                     AARDILVARVPSQAGSVQDQYTAYLAAIQDGPAKANGTAAGPAAAAAVLANREGDG"
     gene            596..1123
                     /locus_tag="VHK28_10380"
     CDS             596..1123
                     /locus_tag="VHK28_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX2142656.1"
                     /translation="MSSPVTVHLAHGASGSAASMARHVDGLRERGLVAEAVQLPRGSA
                     ERALPAYLAAAPPGPDRVIGGHSFGGRVASLAVADHAYAGLVLLSYPLHPPGRPERAA
                     ERTSHWPRIGCPVLLLSGDRDPFARIEVMRDLVEQLPRAELVIYPGLRHGIGPRLEDA
                     LDRIATFVKSLRPDR"
     gene            1159..3399
                     /locus_tag="VHK28_10385"
     CDS             1159..3399
                     /locus_tag="VHK28_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142657.1"
                     /translation="MELVACPSCGEENPSKFRLCGFCGTPLAAALPPQEERKVVTVFF
                     SDLKGSTDLGEALDPESLREVMTRYFDAMTRVLHRHGATIEKFIGDAIMAVFGLPKLH
                     EDDALRAVRAALETREALAELNDELQRTYGVQLINRTGVNTGEVVAGDPTTGQRLVTG
                     DTVNVAARLETAAPPNEILIGALTYRLVRGAVEVEEVEPLELKGKAERVPAYRLIAVS
                     DATEGFERRQDGPMVGRDREMTALSATLERALTERRCRMATVVADAGVGKSRLVREFT
                     DAHADAANVVRGRCLPYGEGITFWPLVEAAREAALIEGDDPPETALAKLQDLTGDDEV
                     TARLASAIGLSGEQYPVAEIFWAARRFLESLGRERPVIVVVDDIHWAEPTFLELIGHL
                     VETVEDASVLLLCTSRPDLLERHPDWATDADSQRLVLSPLTDADAAQVVGGLLGEAGI
                     AGEAQERIVRAAEGNPLFVEQLLSMLIDTGALHFTDGRWEPTGDLADIDVPPSIQALL
                     AARLDLLGREERAVIEPASVIGLTFAQAAVAALAPDAVRPKVPDHLTSMTRKQLVKPN
                     RVATAAAGEMRHLHPRPMMPPTASSTSSSATRPTARSSSGFAPPTTRPSPTGPMSSIA
                     DRAGPRSTRRSWATTWSRRTCIGPSWGRWTTTDVPSALALPRSWLPPADAPWPAATCR
                     PRPTCCAGPQRSFPRMSWDAWHCSPTWERRSPSWASSRRRSASWARRVRAPRAWMTSR
                     WPRVHR"
BASE COUNT          482 a         1227 c         1227 g          502 t
ORIGIN      
        1 cccgtcgccc tcgcggttgg cgagcacggc ggcggccgcg gccgggccgg ccgcggtgcc
       61 gtttgccttg gcaggtccgt cctggatggc ggccaggtat gccgtgtatt ggtcctgtac
      121 cgatccggcc tggctcggga cgcgcgcgac caggatgtcg cgcgctgcgg ccgccacagc
      181 cgcatcggga ttcgccccgt caccggtcgg cgagctgatg aacggctccc ctccgccgtc
      241 gatcgcgacg acggcgtcgt agatggcggc gtgaacgagt gcggcgacgt acatgccgct
      301 cgtgcctggt cggccaaccg accacgcgtt ctgggcaatc aggctccagt cggtgacggc
      361 gttgccctcg gccgcagccg cgagcggcag cctggctgga gcgccggcca ccaggccgct
      421 gaaggctatg acgacagcga tgatggccgt catcgggcgc gcgagggtct tcattccatc
      481 tctcctggat gcgttggcgg ttcgccgcgc cgagtacccg ccgcgcggac cacgcctacc
      541 ctctatgggt ccggaaacgg gccggcggtt caatcaacgc cgctaggctg tcgcggtgag
      601 ctccccggtc accgtccacc tggcgcatgg cgccagcggc agcgccgcct cgatggcccg
      661 gcacgtggac ggcctgcgcg agcgcggact cgttgccgag gccgtgcagt tgccgcgcgg
      721 cagcgccgag cgggcgctgc cggcctacct ggccgccgca ccacccggcc cggaccgggt
      781 catcggcggg cattccttcg gcggccgggt cgcctcgctg gcggtcgccg accatgccta
      841 cgccggcctg gtgctcctct cctacccgtt gcacccgcca ggtcggccgg agcgtgccgc
      901 ggagcggacc tcgcactggc cgcgcatcgg ctgcccggtc ctgctgctct cgggcgaccg
      961 cgacccgttc gcccgcatcg aggtcatgcg cgacctggtt gagcagctgc cccgggctga
     1021 gctcgtcatc tacccggggc tgcgccacgg catcggtcca aggctggagg atgccctgga
     1081 tcgcatcgca accttcgtga agtcgctcag gcccgaccgc tagggctacc cgtcgcgtca
     1141 tacactccgc gggtgacgat ggagctggtc gcctgcccca gctgcggcga ggagaacccg
     1201 agcaagttcc gtctctgcgg gttctgcggt acgccgctgg ccgctgctct gccgccccag
     1261 gaggagcgca aggtcgtcac cgtcttcttc agcgacctca agggctccac cgatttgggc
     1321 gaggcgctcg atcccgagag cctgcgcgag gtgatgaccc gctacttcga cgcgatgacc
     1381 cgcgtcctgc accggcacgg ggcgaccatc gagaagttca tcggcgacgc gatcatggcc
     1441 gtcttcgggt tgcccaagct ccacgaggac gatgccctcc gcgcggtgcg cgccgcactg
     1501 gagaccaggg aggcgctggc cgagctgaac gacgagctac agcgcaccta cggcgtgcag
     1561 ctgatcaatc gcaccggcgt caacaccgga gaggtagttg ccggtgatcc gacaaccggc
     1621 cagcggctgg tcacgggcga cacggtcaac gtggcggccc gcctggagac ggcggctccg
     1681 cccaacgaga tcctgatcgg ggccctcacc taccggctcg tgcgcggcgc ggtcgaagtc
     1741 gaggaggtcg agccgctcga gctgaagggg aaggccgagc gagtccccgc ctaccgcctg
     1801 atcgccgtct cggacgcgac cgagggcttc gagcgccggc aggacggacc gatggtcggg
     1861 cgcgaccgcg agatgacagc actgtcggcc acccttgagc gtgccctgac tgagcggcgc
     1921 tgccggatgg ccaccgtggt ggccgatgcc ggcgtgggca agtcgcggct ggtccgcgag
     1981 ttcaccgacg cccatgctga cgcagcgaac gtggtgcgcg gacgctgcct gccgtacggg
     2041 gagggcatca ccttctggcc gctggtcgag gccgcgcgcg aggcggcgct gatcgagggc
     2101 gacgacccac cggagaccgc gcttgccaag ctgcaggacc tgaccggcga cgacgaggtg
     2161 accgcccgcc tggcatcggc catcggtctc agcggcgagc agtacccagt ggccgagatc
     2221 ttctgggccg ctcgccgctt cctggagagc ctgggccggg agcggccggt gatcgtggtg
     2281 gtcgacgaca tccactgggc cgagccgacc ttcctggagc tcatcggtca cctggtggag
     2341 accgtcgagg acgcatcggt cctgctgctg tgcacgtcgc gtcccgacct gttggagcgt
     2401 catcccgact gggcgaccga cgccgacagc cagcgactgg tgctcagccc gctgaccgat
     2461 gccgacgcgg cccaggtcgt cggcgggctg ctgggcgagg ccggcatcgc cggggaggcg
     2521 caggagcgca tcgtgcgcgc cgcggagggc aatcccctgt tcgtcgagca gctgctgtcc
     2581 atgctgatcg ataccggcgc gttgcacttc accgacggtc gctgggagcc caccggcgac
     2641 ctggccgaca ttgacgtgcc gccgtccatc caggccctgc tggcggcccg gctcgacctg
     2701 ctggggcgcg aggaacgcgc ggtgatcgag ccggccagcg tgatcggcct gaccttcgcg
     2761 caggccgcgg tggctgcctt ggcccccgac gcggtgcggc ccaaggtgcc cgaccacctc
     2821 accagcatga cccgcaagca gctggtcaag ccaaaccgtg tggcgacggc tgcggcgggc
     2881 gagatgcgtc acctgcatcc gaggccgatg atgccgccta ccgcttccag cacctcctca
     2941 tccgcgacgc ggcctacggc gcgctcctca agcggattcg cgccacctac cacgaggcct
     3001 tcgccgactg ggccgatgag ctcaatcgcc gacagggcag gtcccaggag tacgaggaga
     3061 tcctgggcta ccacctggag caggcgtacc tgtatcgggc cgagctgggg acggtggacg
     3121 accacggacg ttccatcggc gctcgcgctg ccgagaagct ggctgccgcc ggccgacgcg
     3181 ccatggcccg cggcgacatg ccggccgcgg ccaacctgct gcgcagggcc gcagcggtcc
     3241 ttccccagga tgagctggga cgcctggcac tgctccccga cctgggagag gcgctcaccg
     3301 agctgggcga gttcgaggag gcgcagcgcg tcctgggcga ggcgcgtgag ggcgccgcgc
     3361 gcctggatga cgagccgctg gccgcgcgtg catcgctgat ccggctgctg gtgaacctct
     3421 acgcctccga ggacgtgg
//
LOCUS       DATACA010000514         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2640960, whole genome shotgun sequence.
ACCESSION   DATACA010000514 DATACA010000000
VERSION     DATACA010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2640960"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /gene="rplI"
                     /locus_tag="VHK28_10390"
     CDS             <1..374
                     /gene="rplI"
                     /locus_tag="VHK28_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX2142658.1"
                     /translation="NFLLPNGAAVVADAGAIKNWERHRSEREERDRSERAAAEATAER
                     LRELKLEIAVKAGEKNRLFGSVTNREIAELLAREGVDIDRHAIHLHEPIKTVGEHRVD
                     VRLMSGVDAQLSVKVIPAPAS"
     gene            589..1935
                     /gene="dnaB"
                     /locus_tag="VHK28_10395"
     CDS             589..1935
                     /gene="dnaB"
                     /locus_tag="VHK28_10395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX2142659.1"
                     /translation="MSIDKLAPQAVEAEQSVLGSILIDPEAILKVADFLRPNDFYRQQ
                     HAEIYEAMLVLHGQREPIDLVTMGDELARRDRLEQVGGPAYLASLMNVVPTAVHVEHY
                     GRIVERKSVLRNLIAAAGKIAAVGYEEASDAEIAIDRAEGILFEVSQRRTDVGFESLS
                     TLLAQAYDRLEYLHEHRGEVLGIPSGLSHLDALLGGFQPSDLIILAARPSVGKTSLAL
                     NIAQHAAVREAKKVAVFSLEMSREQLALRLLSAESGINPRPLQSGFVDETDWSKIAQV
                     MNSMHNAPMWIDDSPILTVMELRTKARRLEAEQHGLDLIIIDYLQLMQGSVQQKDANR
                     VQEVSEISRGLKSLARELKVPVIALSQLSRNVEQRGTQEPRLSDLRESGSLEQDSDVV
                     IFLYREGEQNPESEVELVKAKVAKHRNGPIGEVPLQFRKSNTRFYTMQREAEVQAY"
     gene            2113..2427
                     /locus_tag="VHK28_10400"
     CDS             2113..2427
                     /locus_tag="VHK28_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX2142660.1"
                     /translation="MYADKTIRCRDCGMDFVFSAGEQQFYAEKGLVNEPQRCPSCRAV
                     AKQNRALGIGSGGGGGTGQREMHAAVCAECGGQALVPFLPRNDRPVYCSSCFDKVRAT
                     RA"
     gene            2541..3902
                     /locus_tag="VHK28_10405"
     CDS             2541..3902
                     /locus_tag="VHK28_10405"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX2142661.1"
                     /translation="MSVIAVVGAQWGDEGKGRVVDYLAHEADLVIRYGGGNNAGHTVV
                     NPHGHFKLHLIPSGIFHPGTRNLLGNGVVIHPPALVAELDGLRAAGFDCANLYISDRA
                     HIVLPHHVLMDQLEERERGKAKLGTTSRGIGPAYVDKVARRVIRMVDLVEPDAFRQRL
                     DAVLRRARRMVEGYFGAGGPEDDLREAIAEALDPERVAAAYLSAGERLGPHVVDGQAM
                     VDAALRGAERILLEGQLGTMRDIDWGIYPYVTSSSPIPGGASLGAGLPAVAIDRVLGV
                     AKAYTTAVGSGPLPTELDDDAGEHLRDRGVEYGTSTGRPRRCGWYDAVAVRFSARLAG
                     YSSIALTKLDVLDELASIPVCVAYRDPADGRTWDTVPASASVYERLEPIYEELPGWQA
                     DTTACRSWNGLPLKAQEYVARLEALAGVAISHVSVGPEREQMIERVESGPLRARNLQE
                     QRA"
     gene            3911..>4118
                     /locus_tag="VHK28_10410"
     CDS             3911..>4118
                     /locus_tag="VHK28_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2142662.1"
                     /translation="MIVPQGWTGEYDGWEPPRAWARTVAVARQAEGLGAESIWLFDHF
                     HTVPRPTDEITFESFTSLSALAAET"
BASE COUNT          679 a         1404 c         1448 g          587 t
ORIGIN      
        1 gtaacttcct gctgccgaac ggcgccgccg tggtggcgga cgccggcgcg atcaagaact
       61 gggagcggca ccgttcagag cgcgaggagc gcgaccgctc ggagcgcgcc gccgccgagg
      121 cgacggccga gcgcctgcgc gagctgaagc tcgagattgc cgtcaaggct ggcgagaaga
      181 accgtctgtt cggctcggtc accaaccgcg agatcgccga gctgctggcg cgcgaggggg
      241 tcgacatcga ccgccatgcg atccacctgc acgagccgat caagaccgtc ggagagcatc
      301 gcgtcgacgt gcggctgatg tcgggcgtgg atgcccagct gtccgtgaag gtcatccccg
      361 ccccggcgag ctaggtaacc gggcgcagtc gcgactgtgg cctgacggtc gcgacgccca
      421 agctgtcata ttccggccgc accctgcgcg catgcgcagc atccggcccg ggcaccggcg
      481 gttttccaca aggggcggga ttgtccacaa cccgccccac ttatccacgc ctccagggcc
      541 gtggagggct cgcttcggcc ggcggaacgc cgcacagtca gcgccgatat gagcatcgac
      601 aagctggccc cccaggcggt cgaagccgag cagagcgtgc tcggctcgat cctgatcgat
      661 ccggaggcga tcctcaaggt cgccgacttc ctgcggccga acgacttcta ccgccagcag
      721 cacgccgaga tctacgaggc catgctcgtg ctgcacggtc aacgggagcc gatcgacctg
      781 gtgaccatgg gcgacgagct cgcccggcgg gatcgcctgg agcaggtcgg ggggcccgcc
      841 tacctggcct cgctgatgaa cgtggtgccc acggcggtcc acgtcgagca ctacgggcgg
      901 atcgtcgagc gcaagtcggt gcttcgcaac ctgatcgcgg ccgcgggcaa gatcgccgcg
      961 gtcggctacg aggaggcgag cgacgccgag atcgccatcg atcgcgccga gggcatcctg
     1021 ttcgaggtaa gccagcgccg gacggacgtc ggcttcgagt cgctctcgac gctgctggcg
     1081 caggcctatg accggctcga atacctgcac gagcatcgtg gcgaggtgct cggcattcca
     1141 tccggcctgt cgcacctgga cgcgctgctc ggtggcttcc agccgtctga cctgatcatc
     1201 ctggccgcgc gccccagcgt gggcaagacc agccttgcgc tgaacatcgc ccagcacgcg
     1261 gccgtgcgcg aggccaagaa ggtcgccgtc ttctcgctgg agatgagccg cgagcagctc
     1321 gccctgcggc tgctgtccgc cgaatcgggg atcaacccgc ggcccctgca gagcggcttc
     1381 gtcgacgaga cggactggtc gaagatcgcg caggtcatga actcgatgca taacgcgccg
     1441 atgtggatcg acgactcgcc catcctgacc gtgatggagc tgcgcaccaa ggcgcgccgg
     1501 ctcgaggccg agcagcacgg gctggacctg atcatcatcg actacctgca gctgatgcag
     1561 ggttcggtgc agcagaagga cgccaaccgc gtccaggagg tgagcgagat cagccgcggc
     1621 ctgaagtcgc tggcccgcga gctgaaggtc ccggtcatcg ccctctctca gctgtcgcgg
     1681 aacgtggagc agcgcggaac gcaggagccg cggctctccg accttcgtga gtcgggcagc
     1741 ctcgaacagg actcggacgt cgtgatcttc ctgtaccgcg agggcgagca gaatccggag
     1801 agcgaggtcg agctggtcaa ggccaaggtg gccaagcacc gaaacggccc catcggcgag
     1861 gtcccactcc agttccgaaa gtcgaacacc cgcttctaca ccatgcagcg ggaggccgag
     1921 gtccaggcct attgagcatg ctcaccaccc gggcgagcgt ctatacagtc cttgaactcg
     1981 cgtgtatagt cgggcgctga acgtccggtc caccgctcgc accccaaacc tgtcgcacgc
     2041 attcggccca gggtccgggg acagcaaccg tccaattccg gcccgggcgt gcgaacagga
     2101 ggctagggaa gggtgtacgc cgacaagacc atccgctgtc gagactgcgg gatggacttc
     2161 gtcttttcag ccggcgagca gcagttctac gccgagaagg ggctcgtgaa cgagccgcaa
     2221 cggtgcccga gctgtcgcgc ggtggcaaag cagaaccgcg cgctggggat cgggtccggc
     2281 gggggcgggg gaactggcca gcgtgagatg cacgccgccg tctgcgcgga gtgcggtggc
     2341 caggccctgg tgccattcct gccgcgcaac gaccgaccgg tctactgctc cagctgcttc
     2401 gacaaggtgc gcgccacccg agcctgagcc actggcccag atcaaaccac gctggaccga
     2461 tgcgagcgcc gcgagcgcgg cgctcgttcg ctgtccaccg ctaccatgac cgacgcccgc
     2521 agaggaacat cagcccgcgc atgtccgtga tcgccgtggt cggcgcccag tggggcgacg
     2581 agggaaaggg ccgtgtcgtc gactacctgg cgcacgaggc ggacctcgtg atccgctacg
     2641 gcgggggtaa caacgccggg cacacggtgg tcaaccccca tggccatttc aagctgcacc
     2701 tgatcccctc cggcatcttc catccgggca cccgcaacct gctgggcaac ggcgtggtca
     2761 tccacccgcc ggcgctggtg gccgagctgg acgggctgcg cgccgccggg ttcgactgcg
     2821 ccaacctgta catctccgat cgggcccaca tcgtgctgcc gcaccacgtg ctgatggacc
     2881 agctcgagga gcgcgagcgc ggcaaggcca agctgggaac caccagccga ggcatcggtc
     2941 cggcctatgt cgacaaggtg gcccgccgtg tcatccggat ggtcgacctg gtggagcccg
     3001 atgccttccg gcagcgcctg gacgccgtcc tgcggcgcgc gcggcggatg gtggagggct
     3061 acttcggcgc gggcggcccg gaggacgatc tgcgtgaggc gatcgccgag gccctggacc
     3121 cggagcgcgt cgccgccgcc tacctgtccg ccggggagcg gctcggtccg cacgtggtcg
     3181 atgggcaggc catggtagat gcggcactgc ggggcgccga gcggatcctc ctggaggggc
     3241 agctgggcac catgcgagac atcgactggg gcatctaccc gtacgtcacc agctccagcc
     3301 cgatcccggg cggcgccagc ctgggcgccg ggctgccggc ggtggccatc gaccgcgtgc
     3361 tgggggtggc caaggcctac accaccgcgg ttggctccgg gccgctgccc accgagctgg
     3421 atgacgacgc gggcgagcac ctgcgtgacc gcggcgtgga gtacggcacc tccaccggcc
     3481 gcccgcgccg ttgcggctgg tacgacgcgg tggccgtgcg cttcagcgcc cggctggccg
     3541 ggtacagcag catcgccctc accaagctgg acgtgctgga tgagctggcc agcatcccgg
     3601 tgtgcgtcgc gtaccgggac ccggccgatg ggcggacgtg ggatacggtc ccggcgtcgg
     3661 catcggtcta tgagcgcctg gagccaatct acgaggagct gccaggctgg caggctgaca
     3721 ccaccgcctg ccggtcctgg aatggcctgc cattgaaggc gcaggagtac gtcgcccgcc
     3781 tggaggcgct ggccggcgta gccatcagcc acgtgagcgt ggggccagag cgcgagcaga
     3841 tgatcgagcg ggtcgagagc ggaccgctgc gggcgcggaa cctgcaagag cagcgggcat
     3901 gaagctgggc gtgatcgttc cccagggctg gaccggggag tacgacgggt gggagccgcc
     3961 gcgagcctgg gcgcggaccg tggcggtcgc ccgccaggcc gaggggctgg gcgccgaatc
     4021 gatctggctc ttcgaccact tccacaccgt tccgcgcccg accgacgaga tcaccttcga
     4081 gtccttcacc tcgctcagcg ccctggcggc cgagacca
//
LOCUS       DATACA010000515         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1036110, whole genome shotgun sequence.
ACCESSION   DATACA010000515 DATACA010000000
VERSION     DATACA010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036110"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1128)
                     /locus_tag="VHK28_10415"
     CDS             complement(115..1128)
                     /locus_tag="VHK28_10415"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX2142663.1"
                     /translation="MPDVPDSHVPDSHVKVAVTGAAGQIGYALLFRIASGQMFGPRTT
                     VDLQLLELEAALPALEGVNMELEDCAFPLLRKVVRTSDPNQAFAGANWALLVGAVPRK
                     AGMERKDLLNVNGGIFGAQGRAIAANAAPDVRVLVVGNPCNTNCLIARASAPEIPTDR
                     WFAMTMLDENRGRSLLARRADVPVTEVRHLAVWGNHSATQFPDFYHASIKGIPAPEVL
                     DEGWLQDEFMTTVQQRGAKIIEARGQSSAASAANAVVDTVRNITQPSGEIFSAAIPAP
                     ADGSGYAIPEGIVFGYPLRATEPGRVEIVQGIRHGEWAQAKIDATRDELLEERAAVQE
                     LVA"
     gene            complement(1155..1676)
                     /locus_tag="VHK28_10420"
     CDS             complement(1155..1676)
                     /locus_tag="VHK28_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2142664.1"
                     /translation="MSQVNLCPHCATPSERPISCQRCGWRWYANPMPAAGTLVERIGT
                     DGSPQVLLLRRAVNPGLGAWDLPAGYLEANESPEDAARRETAEEAGFEVELLSLAGVY
                     TSREGNAVSVIYLARPLVPEAATVRPDAESDAHAWVGRGDVERWLPLMAFPAMAAALE
                     DWARGVTGMPREW"
     gene            1797..>3055
                     /locus_tag="VHK28_10425"
     CDS             1797..>3055
                     /locus_tag="VHK28_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX2142665.1"
                     /translation="MESIGPASRLNTAGSPFPPIASYGFLSDCETTALVAPSGNVEWL
                     CLPRMDSPSVFGAILDRDAGSFRLGPADVRVPAHRRYVPGTMVLETSWSTRTGWIIVR
                     DVMLIGPWHSEERRTHTHKRAPTDYDAEHILLRSVRCVNGSVQLVMECEPRFDYGRSF
                     GEWSYTEDGYRQGRCRCPDTDVELTLTGDLNLGFEGPKAIARTLMKEGETRFVALSWG
                     GVKPPRTGDDAYGRLVWTAHHWQHWLARGHFPDHPWRSYLERSALTLKGLTYAPTGAV
                     AAAPTTSLPETPGGERNWDYRYSWIRDSTFALWGLYRLGFDWEATDFIFFIAEVARAD
                     KNLQIMYGIGGERELPERTLDHLAGYEGVRPVRVGNAAYAQRQHDMWGALLDSVYLHT
                     KSRDHLDESIWPIIMEQVETALEHWREP"
BASE COUNT          446 a         1071 c         1060 g          478 t
ORIGIN      
        1 gcagccgtga gatgtcaacg gccccctcat cctccgtgcc atggatgacc ggcatctcca
       61 gctccttgcc gtcgaaggcc agtcgaacgg tgtcggtgcg cggggcggtt tcggtcatgc
      121 caccagctcc tgcacggccg cgcgctcctc cagcagctcg tcccgggtgg cgtcgatctt
      181 cgcctgcgcc cattcgccgt gccggatgcc ctgcacgatc tccacccgtc ccggttcggt
      241 ggcgcgcagc ggatagccga agacgatgcc ctccgggatg gcgtacccgg agccgtccgc
      301 cggagcggga atggccgcgc tgaagatctc gccgctcggt tgcgtgatgt tgcgcaccgt
      361 gtcgaccact gcattagccg cactcgccgc actcgactgt ccgcgtgctt cgatgatctt
      421 cgcgccgcgc tgctgcaccg tggtcatgaa ctcatcctgc agccagccct cgtccagcac
      481 ctccggcgcc ggaatgccct tgatcgacgc gtggtagaag tccgggaact gcgtggccga
      541 gtgattgccc cacacggcca ggtggcgcac ctcggtcacc ggcacgtccg cgcgacgggc
      601 cagaaggctg cggccgcggt tctcgtccag catggtcatg gcgaaccatc ggtcggtggg
      661 gatctcgggc gcgctggcgc gggcgatcag gcagttggtg ttgcagggat tgcccaccac
      721 cagcacgcgc acgtcgggtg ccgcgttggc cgcgatcgcc cgcccctggg cgccgaagat
      781 gccgccgttg acgttcagca ggtccttgcg ctccatgccc gccttccgcg gcaccgcacc
      841 taccagcagg gcccagttgg cgccggcgaa ggcctggttc gggtccgagg tgcgcaccac
      901 tttgcgcagc agggggaagg cgcagtcctc gagctccatg ttcacgccct ccagcgccgg
      961 cagggcggcc tcgagctcga ggagctgcag gtccaccgtg gtgcgcgggc cgaacatctg
     1021 gccggaggca atccggaaca ggagggcgta gccgatctgg cctgcggcgc ctgtcaccgc
     1081 gaccttgacg tggctgtcgg ggacgtggct gtcgggaacg tcgggcatcg gatcgagtgt
     1141 agcgcagccc gtgctcacca ctcccgcggc atgccggtca cgccgcgagc ccagtcctcc
     1201 agggcggcgg ccatggccgg gaaggccatg agcggaagcc acctctccac atccccgcgc
     1261 ccgacccatg cgtgcgcgtc ggattccgcg tccgggcgaa ccgtggcggc ctccggtacc
     1321 agcggccgcg ccaggtagat gacgctcacc gcgttgccct ccctggaggt gtagacgccg
     1381 gccagcgaca gcagctcgac ctcgaagccg gcctcctcgg cggtctcgcg ccgagcggca
     1441 tcctccggcg actcgttggc ctccaggtag ccggcgggca ggtcccacgc cccgaggccg
     1501 gggttgacgg cgcggcgcag cagcaggacc tgcgggcttc cgtcggtacc gatgcgctcc
     1561 accaacgtcc ccgcggccgg catggggttg gcgtaccagc gccagccgca gcgctggcag
     1621 gagatcggcc gctcggaggg cgtggcacag tgcgggcaga ggttgacctg gctcatcggc
     1681 gatcagccta tcggaccggt tggcacgccg cgggctatgg tcaactcgca tcggacgtgg
     1741 taagtgttga tgcttacctg gaggtgagtc ggatgcatga gggcgccaac gggcggatgg
     1801 agtcgatcgg tcccgccagc cggttgaaca ctgcgggctc gccgttcccg cccatcgcga
     1861 gctacggctt cttgtccgat tgcgagacca ccgcgctggt cgccccgtcc ggcaacgtgg
     1921 aatggctatg cctgccgcgc atggactcgc catctgtctt cggcgccatc ctggatcgcg
     1981 acgccggcag cttccggctg gggcctgccg acgtgcgggt gcccgcccac cgccgctacg
     2041 tgccgggcac catggtcctc gagaccagct ggagcactcg gacggggtgg atcatcgtcc
     2101 gcgacgtgat gctcatcggt ccatggcaca gcgaggagcg tcgcacgcac acccacaagc
     2161 gagcgcccac cgactacgac gcggagcaca tcctgctgcg gagcgtgcgc tgcgtgaacg
     2221 gatccgtgca gctggtgatg gagtgcgagc cgcgcttcga ttacgggcgc tccttcggag
     2281 agtggtcgta caccgaggac ggctaccggc aggggcgctg ccgctgcccg gacaccgacg
     2341 tggagctgac cctgaccggc gacctcaacc tcgggttcga gggtcccaag gccatagccc
     2401 gcaccctgat gaaggagggc gagacccgct tcgtggccct ctcctggggt ggcgtcaagc
     2461 cgccgcgcac cggggatgat gcctacggtc gcctggtgtg gaccgcgcac cactggcagc
     2521 actggctggc gcgcggccac ttcccggacc atccgtggcg cagctacctc gagcggtcgg
     2581 ccctgacgct gaagggcctg acctacgccc cgaccggcgc ggtggcggcg gcgcccacga
     2641 cttcccttcc cgagaccccc ggtggcgagc gcaactggga ctaccgctac tcctggatcc
     2701 gcgactccac cttcgcgctg tggggcctgt acaggctggg cttcgactgg gaggcgaccg
     2761 acttcatctt cttcatcgcc gaggtggcgc gggccgacaa gaacctgcag atcatgtacg
     2821 gcatcggtgg tgagcgtgag ctgccggaga ggaccctgga ccacctggcc ggctacgagg
     2881 gagtgcggcc ggtgcgggtc gggaacgcgg cgtacgcgca gcgccagcac gacatgtggg
     2941 gcgcgctcct ggactcggtg tacctgcaca ccaagtcgcg cgaccacctg gacgagtcca
     3001 tctggccgat catcatggag caggtcgaga cggccctgga gcactggcgc gagcc
//
LOCUS       DATACA010000516         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1598425, whole genome shotgun sequence.
ACCESSION   DATACA010000516 DATACA010000000
VERSION     DATACA010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598425"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..653
                     /locus_tag="VHK28_10430"
     CDS             231..653
                     /locus_tag="VHK28_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142666.1"
                     /translation="MTSAPQSPLAAALERVGDRWSLLLVEALLQGPRRFGELGESVPG
                     IAPNILADRLRRLEAERVVRSQPYSERPPRFVYELTAEGAELAGALRLLADWGSRVAR
                     DAEPMRHAACGTPLEARWFCTTCARAVEGTEAEEVRYL"
     gene            complement(650..>1832)
                     /locus_tag="VHK28_10435"
     CDS             complement(650..>1832)
                     /locus_tag="VHK28_10435"
                     /inference="COORDINATES: protein motif:HMM:NF025391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HEX2142667.1"
                     /translation="HPRIPGSDSTDPPGCERPEQPVTLEPDGEIVERWELPFDITSAR
                     VLRAVGSSGSVVRAEVVEARDRNELEFLDIYSTGAPEAAEARRGRAVWVETPAEAVVK
                     REPDAQPAALSLGQLFDRLMEDAELRAWIEAQPADAWRQAALTPTFAALGDVEEQRVE
                     LRLITAEYERAALVSARHDGGGAELQLPGEEWRTRAFERRAATLPPGIRLIDEPDGYT
                     PSDDLLLGELMLPSGRIAVGEFLFDEEELEIVVAAGSYPVHATLARYQGQEHDNVALA
                     TLILSPRQTVRWELAYTFPVDGGTATFVSAEGRDLLSGQLDADESAWRALDQEVFDSM
                     MAHDYLGTEWQVDGGLNLVRVSSGVGDGGYPVYVGFDAGGQPTMVVVDFYLLHLAWPS
                     G"
BASE COUNT          251 a          683 c          624 g          274 t
ORIGIN      
        1 ggtcagcacc tttccgttct cgatcaccac gccggatccg ccgcgccatc ggttcccgat
       61 gccgacggtc gaccttcccg ccgtctctgc cacgcccgcc agggcggttc caagctgctc
      121 gatgggaccc atcggtccat cctcctgtct gcaacgctag aatgtgactt gcaatgtgca
      181 agttacatgc acctggccgg ctgtcaagcc cgatgacgta cgatccgcgc gtgacctccg
      241 cgccacagtc accactggcc gccgcgctcg agcgggtggg ggaccgatgg agcctcctgc
      301 tggtcgaggc gctgctccag ggcccgcgcc gtttcggcga gctgggggag tcggtgcccg
      361 gcatcgcccc gaacatcctg gccgaccggc tgcgccggct ggaggccgag cgggtggtgc
      421 gttcgcagcc ctactcggag cgtccgccgc gcttcgtcta cgagctcacc gcggaagggg
      481 ccgagctggc cggggccctc cggctgctgg ccgactgggg ctcacgcgtg gcccgggacg
      541 ccgagccgat gcgccatgcc gcctgcggca ccccgctcga ggcgcgctgg ttctgcacca
      601 cctgtgcccg ggccgtggaa ggcaccgaag ccgaggaggt ccgctacctc tagccggagg
      661 gccaggcgag gtgcagcagg tagaagtcga cgaccaccat cgtcggctgg ccgcctgcat
      721 cgaacccgac gtacaccggg tagccgccgt cgcccacgcc gctgctgacc cggaccaggt
      781 tcaggccgcc atcgacctgc cactcggtgc ccaggtagtc gtgggccatc atcgaatcga
      841 agacctcctg gtcgagcgcg cgccaggcgg attcgtcagc gtccagctgt cctgacagca
      901 ggtcacgtcc ctccgccgag acgaatgtgg ccgtgccacc gtccaccggg aaggtgtagg
      961 ccagctccca gcgcacggtc tgcctcgggg agaggatgag ggtggccagc gccacattgt
     1021 cgtgctcctg tccctgatag cgggccagcg tggcgtgcac cgggtacgag cctgccgcca
     1081 cgacaatctc cagctcctcc tcgtcgaaca ggaactcgcc caccgcgatg cggccggacg
     1141 gcagcatcag ctcgccgagc aggaggtcgt ccgagggggt gtagccgtcc ggctcgtcga
     1201 tgagccggat cccgggtggg agcgtcgccg cgcgccgctc gaaggcacgc gtccgccact
     1261 cctcaccggg cagctgcagc tccgcgccgc cgccgtcgtg cctggcgctg accagcgccg
     1321 cgcgctcgta ctcggcggtg atcagccgca gctccacgcg ctgctcctcg acatcgccca
     1381 gggcggcgaa ggtcggcgtc agcgcggctt ggcgccaggc atcggccggc tgcgcctcga
     1441 tccaggcgcg cagctccgcg tcctccatga gtcgatcgaa cagctggccc aggctgaggg
     1501 cggcgggctg cgcatccggc tcccgcttga cgaccgcctc ggccggcgtc tcgacccaga
     1561 cggcacgtcc gcggcgcgct tcggctgcct ccggtgcgcc ggtcgagtag atgtccagga
     1621 actcgagctc gtttcggtcc cgcgcctcga ccacctcggc gcgcaccacg ctgccggacg
     1681 agccgaccgc gcgcagcact cgggcgctgg tgatatcgaa cggcagctcc cagcgctcca
     1741 cgatctcgcc gtccggctcc agcgtgaccg gctgctccgg gcgctcgcat ccggggggat
     1801 cggtcgaatc gctgcccggg atgcgcgggt gg
//
LOCUS       DATACA010000517         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1517354, whole genome shotgun sequence.
ACCESSION   DATACA010000517 DATACA010000000
VERSION     DATACA010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517354"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VHK28_10440"
     CDS             <1..201
                     /locus_tag="VHK28_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEX2142668.1"
                     /translation="VAAVRRGTRLATAMESRGFGARQCRTVARPQVVRQSDLVLVAAA
                     AALGLGAVGVSLATGSWRFIFG"
     gene            complement(205..1506)
                     /locus_tag="VHK28_10445"
     CDS             complement(205..1506)
                     /locus_tag="VHK28_10445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2142669.1"
                     /translation="MDLPASAFSPPPEPVPFVPVEGAELPSSAEVAVVGAGMVGAMAA
                     LRLAQRGMRPVVIEANAPASGASGRLAGMALAGLGGHFPRVTRLVREASGRSVADYTQ
                     RSLDLLEELEAELPGGFGWQRSGSVDLLLSEAEERHGRQLAELRSAEGLEVQILGPDE
                     LREVAPSVAPGSVRAARWTPGDGQLHPIRMVYALLAEATRLGASVVTGVRVERVLARS
                     GRVFGVATSHGELPAGAVLLATNAWSGWLVPQVADNLTPIREHVCVTEPLPPLLASGF
                     ETNQCNEYWRQLASGEILIGGFTAADEGSGIGSYSMEVRPQVPPLLAELLGRIHPAAA
                     DARIVRCWAGLLDFASLEVPMVGRLPAADGTPLPGGYLACGLTGHGLPYAPILGVLLS
                     ELIAEGEARTMPLEPIDPARYVGVHHAPTWLEPFSGMSAVS"
BASE COUNT          223 a          589 c          554 g          197 t
ORIGIN      
        1 gtggccgccg tgcggcgtgg aacgcgactg gcgaccgcaa tggagtcccg gggcttcggc
       61 gctcggcagt gccgcacggt ggcccgcccg caggtcgtgc gccagtccga cctggtgctt
      121 gtcgcggccg ccgcggcgct cggtctcgga gcggttggcg tcagcctggc cacgggctcg
      181 tggaggttca tcttcggttg aggctcagct gaccgcggac atgccgctga atggctccag
      241 ccaggtcggt gcgtggtgga cccccacgta gcgggccgga tcgatcggct ccagcggcat
      301 cgtccgcgcc tcgccctccg cgatcagctc ggacagcaac acgcccagga tgggagcgta
      361 tggcagccca tgcccggtca ggccgcaggc caggtacccg cccggcagcg gcgtcccgtc
      421 ggccgcgggc aaccggccga ccatgggcac ttccagcgac gcgaagtcca gcaggccggc
      481 ccagcagcgc acgatgcggg cgtccgccgc cgccggatgg atgcgcccga gcagctcggc
      541 cagcaatggg ggcacctgcg gccggacctc catcgagtag gagccgatcc ccgatccctc
      601 gtcagcggcg gtgaagcctc cgataaggat ctcgccgctc gccagctggc gccagtactc
      661 gttgcactgg ttcgtctcga agccggacgc caggagtggt ggcagcggtt cggtgacgca
      721 cacgtgctcg cggatcggcg tcaggttgtc cgccacctgc gggaccagcc agcctgacca
      781 tgcgttggtt gccagcagca cggcaccggc cggcagctcg ccgtgggagg tggccacgcc
      841 gaaaacgcgg cccgatcggg ccaggacgcg ttcaacccgc acgccggtga cgaccgaggc
      901 gcccaggcgc gtggcctcgg ccaggagggc gtacaccatc ctgatcgggt gcagctgccc
      961 atcaccaggc gtccacctgg cggcccgcac cgaacccggg gcgacggacg gcgccacctc
     1021 gcgcagctca tccggtccca ggatctgcac ctcgaggccc tcggctgacc gcagctcggc
     1081 gagctgccgg ccatgccgct cctcagcctc gctgagcagc aggtcgacgg agccgctgcg
     1141 ctgccagccg aacccgcccg ggagctcggc ttccagctcc tccagcaggt ccagcgagcg
     1201 ctgcgtgtag tcggccacgc ttcgccccga cgcctcgcgc accagccggg tcacgcgtgg
     1261 gaagtggcca cccaagcccg ccagcgccat gccggccagc cggccactgg cccccgacgc
     1321 cggcgcattg gcctcgatga ccaccggacg catcccccgc tgcgccagcc gaagcgcggc
     1381 catggcgccg accatgcccg ccccgacgac cgccacctcg gcactggagg gcagctcggc
     1441 accctcgacc ggcacgaagg ggaccggttc cggcgggggg ctgaaggcgg aggcggggag
     1501 gtccatgcgg tggccgagtg taccctcggg ccatgccacg ctcccaggag ctgcgggtgc
     1561 tgg
//
LOCUS       DATACA010000518         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1277836, whole genome shotgun sequence.
ACCESSION   DATACA010000518 DATACA010000000
VERSION     DATACA010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277836"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..141
                     /locus_tag="VHK28_10450"
     CDS             13..141
                     /locus_tag="VHK28_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2142670.1"
                     /translation="MTDATPVGHMVCFLMSDWAAMTTGEMIHVDGGYHAMGTDITW"
     gene            149..799
                     /locus_tag="VHK28_10455"
     CDS             149..799
                     /locus_tag="VHK28_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX2142671.1"
                     /translation="MPRRSVGDHFDEWASRYDAEIREQVPRYDEIQDTVVSLLSLRPP
                     HRVLDLGTGTGVTALRVLEALPQTRVVGLDVSNEMLSRARQRLRAHRRRVELRPGNIA
                     TPELDGLYDAIVSVLAVHHLWADEKRHLFSRLWEHLSPGGILVVADAFRHASDRLVEL
                     YGQPRPEDPHEAEHDHLDTTADHLDWLQAAGFASVDVAWKYEDVGVLVAWKADRRP"
     gene            complement(893..1843)
                     /locus_tag="VHK28_10460"
     CDS             complement(893..1843)
                     /locus_tag="VHK28_10460"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX2142672.1"
                     /translation="MALPIPEPDFPTGPAPNRFVAVETTGIFCRPGCPAPAPRAGNVR
                     PASSAREALFSGFRPCLRCRPLESAGPMPTPAELRRAERLRPVLAAARRARRVRRGSD
                     AVVLRLLSTPLGPMLAGATDDGIALLEFADRPMLPTQLAVLERRLRRPLVAGRHVLLD
                     RLRAELGEYFGGERGTFDVPLVVAGTPFQERVWDVLRAIPTGSTLAYEELAELAGHPS
                     AMRAAGTANGANRLALLIPCHRVIRKSGETGNYGGGRWRKAWLLAHEARLAAATDLSA
                     PDARAPAGRTVLHTGRGLGANLPIYQAAHTTNRQVLRPSL"
     gene            complement(1912..2328)
                     /locus_tag="VHK28_10465"
     CDS             complement(1912..2328)
                     /locus_tag="VHK28_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142673.1"
                     /translation="MAEPGVELRSLLLCDYAITGQDGKVSAIGLFSQLNVSRLPTTYG
                     RLFIVAVLEADSGQHQLTLQVVSPNGEPLLARPPQMRLEVPPNATTANIVADLKGLQV
                     RELGRHRIELRSGDRVLGSTPFNVSLMWQDRRVAKA"
     gene            complement(2373..2864)
                     /locus_tag="VHK28_10470"
     CDS             complement(2373..2864)
                     /locus_tag="VHK28_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX2142674.1"
                     /translation="MILYFLRHADAEPAGAGGDAERRLSPAGEAALRAAWPLWRRLNL
                     RPDAVVSSPLPRALQTAQLLVDGLGLPRPPEADDRLAPGARWADFAQAMTAHPNARRV
                     LFVGHEPDLSSAIGLLTGAASVRMRKGGLACVEFPGTPEPSGGELAWLIDPDLYAEDA
                     PQP"
     gene            complement(2882..3280)
                     /locus_tag="VHK28_10475"
     CDS             complement(2882..3280)
                     /locus_tag="VHK28_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX2142675.1"
                     /translation="MTDATAIPINMYENDREVMLVTPMPGVTAEDISVEVRDDGTVTL
                     RARMHGEGQERIDYLLREWSYGPYERTVELPRAVDANRANLSFGNGVLAVALPKASAT
                     APAMLAVSQTGHTRGMTAGHSGGRGGAASD"
     gene            3368..3811
                     /locus_tag="VHK28_10480"
     CDS             3368..3811
                     /locus_tag="VHK28_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX2142676.1"
                     /translation="MQRSSRPPDELISSLPPDVQLDMRRLDQEISSVMAGLERVVWEG
                     PMWGGTQQNIVGYGAYRYVNRSGNAVDWFMVGLAAQKNHLSLYVNAVDGKEYLVKSHA
                     SRLGKVKVGSANVTFKRLADVDLDALRQMLARARELMTGDAGASA"
     gene            complement(3808..>4491)
                     /locus_tag="VHK28_10485"
     CDS             complement(3808..>4491)
                     /locus_tag="VHK28_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX2142677.1"
                     /translation="KRYRNEYGQLLEHSPYYHRDIRPPDEMPPVTDADGDHVILVRSR
                     DRITPYHYRHHPFDLVGWDGYLWPFAFNIGDFEPITGRIHQPPPVHLTFQPRNFVVCS
                     FVPRKFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVDIGSFTVHPAGIPHGPH
                     PGTVEASIGKEATEELAVMIDTFHPLYLTRQAVEMEDDRYPYSWIPPEDAGEHARELS
                     ERGPEAFPD"
BASE COUNT          647 a         1483 c         1700 g          661 t
ORIGIN      
        1 ctgggctggg ccatgaccga tgccaccccg gtgggccaca tggtctgctt cctgatgagc
       61 gactgggcgg ccatgaccac cggcgagatg atccacgtgg acggtggcta ccacgccatg
      121 gggaccgata tcacctggtg aacggctgat gccccgacgc agcgtgggcg atcacttcga
      181 cgagtgggcg tcgcgctacg acgccgagat ccgcgagcag gtcccgcgct acgacgagat
      241 ccaggacacc gtggtctcgc tcctctccct gcggccgccg caccgggtgc tggacctggg
      301 gaccggcacc ggggtcacgg cgctgcgggt gctggaggcc ctgccgcaga cccgggtggt
      361 cggtttggac gtgtccaacg agatgctgtc ccgggcgcgg caacggctgc gggcgcaccg
      421 gcggcgggtg gagctgcggc cgggcaacat cgccaccccc gagctggatg gcctgtacga
      481 cgccatcgtg tcggtcctgg cagtgcacca cctgtgggcc gatgagaagc gccacctctt
      541 ctcgcgcctg tgggagcacc tctcgccggg cggcatcctg gtggtggccg acgccttccg
      601 ccacgcctcg gaccgcctgg tggagctcta cggccagccg cgtcccgagg acccgcacga
      661 ggcggagcac gaccacctag acacgaccgc cgaccacctc gactggctgc aggccgccgg
      721 cttcgcctcg gtggacgtgg cctggaagta cgaggacgtt ggcgtgctgg tggcctggaa
      781 ggcggaccgc cggccctgag ccgtgtgcgg cgcgacctga cggcgatgta acgggcagcg
      841 gttcacggga agccgccgtg gtgtgttgat ctgccggtgt atcgggcagc cgtcagagtg
      901 aagggcgaag aacttgtcgg ttcgtggtgt gagctgcttg gtagatcggc agattggcgc
      961 ccagcccacg ccccgtgtga agaactgtcc gacctgccgg cgcacgtgca tctggcgctg
     1021 aaagatcggt cgcggcggcc aggcgcgcct cgtgggccag cagccacgcc ttccgccagc
     1081 ggccgccacc gtagttgccc gtctcgccac tcttgcggat cacgcggtgg caggggatca
     1141 gcagcgccag ccggttggcg ccgttggcgg tgcccgccgc gcgcatggcg gacggatggc
     1201 ctgcaagctc ggccagctcc tcgtaggcca gcgtggaccc ggtcgggatc gcgcgcagca
     1261 catcccacac ccgctcctgg aacggcgtcc cggcgaccac cagcggcacg tcgaacgtgc
     1321 cgcgctcacc gccgaagtac tcgcccagct cggcccgcag gcggtccagc aggacatggc
     1381 gtccggccac cagcggccgg cgcaggcggc gctccagcac ggccagctgg gtgggcagca
     1441 tgggacggtc ggcgaactcg aggagggcga tgccgtcgtc ggtggcaccc gccagcatgg
     1501 gtcccagcgg ggtggacagc aggcggagca cgaccgcgtc cgatccgcgc cgcacgcggc
     1561 gcgctcggcg cgcggcagcc aggaccggcc gcagccgctc tgcgcgtcgc agctcggccg
     1621 gcgtgggcat cggtccggcg gactccagcg ggcgacagcg caggcagggg cggaagccgg
     1681 agaagagcgc ctcgcgcgcg gaggatgctg gccggacatt gccggctcgc ggggcgggtg
     1741 ccggacagcc gggtcggcag aagatgccgg tcgtctcgac tgccacgaag cggttggggg
     1801 cagggccggt cgggaagtcg ggctcaggga tggggagggc catggacacc gatcacctct
     1861 catgcagtgc cggtttaaac gcgcgccccc gccggcgcgt gagatcgggg ctcaggcctt
     1921 ggccacccgg cggtcctgcc acatcaggct cacgttgaac ggcgtgctgc cgagcacgcg
     1981 gtcgccggag cgcagctcga tccgatgccg ccccagctcg cgcacctgca gccccttgag
     2041 gtcggccacg atgttggcgg tggtggcatt gggcggcacc tccaggcgca tctgcggcgg
     2101 ccgggcgagc agcggctccc cgttgggcga gacgacctgc agggtcagct ggtgctgtcc
     2161 cgaatcggcc tccagcacgg cgacgatgaa gagccgtccg taggtcgtcg gcaaccggct
     2221 gacgttgagc tggctgaaga ggccgatggc gctcaccttg ccgtcctggc cggtgatcgc
     2281 gtagtcgcac aggagcaggc tgcggagctc cacgcccggt tcggccatgg gccgagtata
     2341 ggacgccgga ccgatgctac cgccgccccc ggtcagggtt ggggtgcgtc ctcggcgtac
     2401 aggtcggggt cgatgagcca ggccagctcg ccgccgctcg gctccggcgt accggggaac
     2461 tcgacgcagg caaggccgcc ctttcgcatg cggaccgatg ccgccccggt caacagcccg
     2521 atcgcggatg acaggtcggg ctcgtgcccc acgaacagga cgcggcgggc gttggggtgg
     2581 gcggtcatgg cctgcgcgaa gtcagcccag cgggcgccgg gggccaggcg atcgtctgcc
     2641 tctggcgggc gcggcagccc cagtccgtca accagcagct gggcggtctg caacgcgcga
     2701 ggcagcgggg agctgacgac ggcgtcgggc cgcaggttca gccggcgcca gagcggccag
     2761 gcggcgcgaa gggcggcttc cccggcgggg ctcagtcgtc gctcggcgtc gccgccggcg
     2821 ccggccggct cggcatcggc gtggcgcagg aagtagagga tcaccgccgt gcgcgtcgcc
     2881 tctagtcgga cgccgcgccg ccgcgcccgc cggagtgccc ggcggtcatg ccgcgggtgt
     2941 ggccggtctg cgacacggcc agcatggccg gagcggtggc ggacgccttg ggcagcgcca
     3001 ccgccaggac gccattgccg aagctgaggt tggctcggtt ggcatcgact gcgcgcggca
     3061 gctcgaccgt ccgctcgtag gggccgtagc tccattccct gagcaggtag tcgatgcgct
     3121 cctgtccctc gccgtgcatc cgggcgcgca gggtgacggt cccgtcgtcg cgcacttcga
     3181 cgctgatgtc ctcggcggtc acgccgggca tgggcgtcac cagcatcacc tcgcggtcgt
     3241 tctcgtacat gttgatgggg atggcggtcg catcggtcat gcgtcaatga acctcactgg
     3301 gagtggccac attctgagga gtggagcagt agcacgtcgc gggccgggtg gctaggcttc
     3361 ggccatcatg cagcgttcct ccaggcctcc cgatgagctg atctcgtcgc tgcctcccga
     3421 cgtgcagctg gacatgcggc gcctggacca ggagatcagc tcggtgatgg ccggcctgga
     3481 gcgggtggtc tgggaggggc cgatgtgggg cggaacccag cagaacatcg tcggctacgg
     3541 ggcgtatcgg tacgtcaacc gcagcgggaa cgcggtggac tggttcatgg tcggcctggc
     3601 ggcgcagaag aaccacctca gcctctacgt caacgccgtg gacggcaagg agtacctggt
     3661 caagagccac gcctcgcggc tgggcaaggt gaaggtcggc agcgccaacg tcaccttcaa
     3721 gcgcctggcc gatgtcgacc tcgacgccct gcgccagatg ctggcccggg ccagggagct
     3781 gatgaccggc gacgcgggcg catcggccta gtccgggaag gcctccggcc cgcgctccga
     3841 cagctcccgc gcgtgctcgc cggcgtcctc cggcgggatc caggagtagg ggtatcggtc
     3901 gtcctccatc tcgacagcct ggcgggtcag gtacagcgga tggaaggtgt cgatcatcac
     3961 cgccagctcc tcggtggcct ccttgccgat ggaggcctcc accgtgccgg ggtgcggccc
     4021 gtgcgggatg cccgccgggt gcaccgtgaa ggaaccgatg tcgacgccgc gccggctcat
     4081 gaagttgccg gccacgtagt agatgacctc gtccgagttg atgttggagt ggttgtaggg
     4141 cgcggggatg gcgagcgggt ggtagtcgaa cttgcgtggc acgaagctgc acaccacgaa
     4201 gttgcggggc tggaaggtga ggtgcaccgg cggaggctgg tggatgcgcc cggtgatggg
     4261 ctcgaagtcg ccgatgttga aggcgaaggg ccacaggtag ccgtcccagc cgaccaggtc
     4321 gaacgggtgg tggcggtagt ggtagggcgt gatgcggtcg cggctgcgga ccaggatcac
     4381 gtgatcgccg tcggcatcgg tcaccggcgg catctcgtcc ggcgggcgga tgtcgcggtg
     4441 gtagtagggc gagtgctcca ggagctgccc gtactcgttg cggtagcgct t
//
LOCUS       DATACA010000519         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2241280, whole genome shotgun sequence.
ACCESSION   DATACA010000519 DATACA010000000
VERSION     DATACA010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241280"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1455)
                     /locus_tag="VHK28_10490"
     CDS             complement(241..1455)
                     /locus_tag="VHK28_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX2142678.1"
                     /translation="MADAAVAWSPAFARRTQGSGGEITAILSLAGAKDIITFSGGFPA
                     PETFPVEVIGELSARLLAEDAAVALQYSPTEGLASVRDTLAQRLAGHEGRRPAEGELM
                     VTSGGIDALQLVGKSLLDAGDVVVVEDPTYLGAIMAFRGFEADVVGIPMDAEGMDVGV
                     LADRLADGLRPKLLYSIPEHQNPTGLTLSTERRQALVELCRRYGVLVLEDVAYRELGF
                     ADERLPSLWSLGPDVVVQAGTFSKTFCPGVRLGWAAGPAAVIGHMVTAKQNTDQCAGA
                     LGQRLMEEYVRGGHLDRQLVASRALYERRCELTMAALEAHMPAGVSWTRPRGGFFTWV
                     TTPGAVDTVAMCQQAAEAKVAYVPGTPFHPGEAGLGSLRLAFSRVAEDDIDEGIGRIA
                     GLLTAGRSAPAS"
     gene            complement(1545..>4560)
                     /locus_tag="VHK28_10495"
     CDS             complement(1545..>4560)
                     /locus_tag="VHK28_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142679.1"
                     /translation="DSWVERRRAVREASVSAPIDQVLPLVQRVAESDADVDVRAAAVR
                     AVGQLEPDLQRAVAGLVAALADPQGPIRLSGALSLLQRPELPLSSQDNEVLWRALVNE
                     RELAIQAALVKLVLARQRDHALVRAESLLDGYDLPSVRAGALIVLLSEEQPDRPALAL
                     RLSAAAGRLGRPTEAFELDRQRLEWAESLTNALNEVMDDPSISDVRVSLILLWLQQIG
                     SLSFETAGDVSMIAEHLAGGVAAWATDVLASEAPAGYGGYLPPSTLTYAQEEVAEALE
                     GVPWLLAEPPQWPDAAIAFLEQLLPAVPEDTRPGIVDALWLVLPRVPRVAQLLFALQG
                     VEGFFDQLPHRATTLDRAGLGRALLVAEEGPYLEQVREHAAAAGPLMIELLHDPEWRF
                     RQQAADWIGKRAARLDSALIDQAAQELARLRDSDPDTDVQNSARAALIEVTGQRRTLS
                     TLPLVVLLRGGDEEKALEALEALDRLPGADAPRAMVGEWVTWLALGERATLVESAAEK
                     LRRKPTSVLPLLDHLAGSLPLDGDLQRVLTASFTPSDILALIDAVRRGQAIPDLDRIR
                     LRKWLGSFETGIARSTAVVLSSEGVDKEELDACIAHELESRRRQREVEVHRRLSRHLM
                     EMSEARFYEGDEATFASVTAQMRLHAVPLLARRLQSEEDVDIRENIARTLGNLGGRAA
                     VDALARAVAGEERTRASRQDLLSRYYLEPSKERSDEAARILTVAVADARQTLRVLQVL
                     NFLFAGVGLVFLAAGFGLLLFSDDPQLRFAGGAISVVSFFGLVIQVIREPLDRIQDAL
                     NRLVQVETAFTSFIWELNLNGTFIQSQYVANGILSNAEITDTVDRIEGAMRLSMDLVS
                     TYADQGATRVIPAIVSVSPGILHDGDRVRVRGRWLSGGRSTKRPQGGQVQIDHRQAAV
                     GVLGWTDSEVEFRFTRGALGTDVSTGVHWLSLSVDGWETNALPVQVVLEGDAQPSSNG
                     AVSGRVAELLVGEGAEPLTASDLSGGDSRNGG"
BASE COUNT          669 a         1659 c         1593 g          639 t
ORIGIN      
        1 ccggcatggc ggcgagaagg tcgctcatgt ccgccaccat gccgctccgc gccgggggcc
       61 agccgacgcc cgtctagtcc ttccgtacac ggtcgatcac actccgttgc gaagccggaa
      121 gcacaaaggc cccgtctccg gttgcgggtc cggacggggc catgtgcgtc ggggtacggc
      181 ttctccatcg gccggcaccc cgcgcacttg agcgctgccg ccggtttggg gcggcttctc
      241 ctaggaggct ggagcgctgc ggccagcggt caggagcccc gcgatgcgtc cgatgccttc
      301 gtcgatgtcg tcctcggcca cgcggctgaa cgcgagccgc agcgagccga gcccggcctc
      361 gcccgggtgg aagggcgtgc cggggacgta ggcgaccttg gcctcagccg cctgctggca
      421 catggcgacg gtgtcgacgg cgcccggcgt cgtgacccag gtgaagaagc cgccgcgcgg
      481 ccgcgtccag ctgacgcctg cgggcatgtg cgcttcgagg gctgccatcg tcagctcgca
      541 gcgacgctcg tacaaggccc tggaggcgac gagctgccgg tccaggtgac cgccccgcac
      601 gtactcctcc atcaggcgtt gcccgagcgc gccggcgcac tggtccgtgt tctgcttcgc
      661 ggtgaccatg tgcccgatca ccgcagcggg accagccgcc cagcccagcc gcacacctgg
      721 gcagaaggtc ttcgagaacg tgcccgcctg gacgacgacg tccgggccga gggaccacag
      781 gctgggcagc cgctcgtccg cgaagccgag ctcgcggtag gcgacgtcct ccagcaccag
      841 gacgccgtag cgccggcaca gctccacgag ggcctgccgg cgctcggtgg acagggtgag
      901 gccggtggga ttctggtgct cggggatgct gtagagcagt ttggggcgca ggccgtcggc
      961 gaggcggtcg gccagcaccc cgacgtccat gccctcggcg tccatcggga tgccgacgac
     1021 atcggcctcg aagccacgga aggccatgat ggcaccgagg taggtggggt cctcgactac
     1081 gacgacgtcc ccggcatcga ggaggctctt gccgaccagc tggagcgcgt cgatgccgcc
     1141 gctggtgacc atcagctcac cctcggcggg ccgcctgccc tcgtgtcccg cgagccgctg
     1201 ggccagcgtg tcacggacgc tcgctagccc ctccgtcggc gagtactgga gtgccactgc
     1261 ggcgtcctcc gcgaggaggc gcgcgctcag ctcgccgatg acctcgacgg ggaaggtctc
     1321 cggcgcagga aagccccccg agaaggtgat gatgtccttc gcgccggcga gggacaggat
     1381 cgcggtgatc tctcccccgg agccctgggt cctccgggcg aacgcggggc tccacgccac
     1441 cgcggcatca gccatcgtca tcagccaacc accctcgagc atcgccgggc cttgcttgtc
     1501 gctgaggata gacctggcaa tggagctgtg gtcgggtggg gacgtcagcc gccgttccgg
     1561 ctgtcacctc cggagaggtc cgaggcagtc agtggttcgg ccccttcacc gaccaggagc
     1621 tcggcaacac gaccgctcac cgcgccgttc gaggaaggct gggcgtcccc ttcgaggacc
     1681 acctgcaccg gcagggcgtt ggtctcccag ccatccacgg acaggctcag ccagtggacg
     1741 ccggtggaga cgtcggtgcc cagcgccccc cgcgtgaagc ggaactcgac ctcgctgtcg
     1801 gtccagccga gcaccccgac ggccgcctgg cggtggtcga tctgcacctg cccgccctga
     1861 ggccgctttg tggagcgccc gccgctgagc cagcgacctc ggacccgcac ccggtcgccg
     1921 tcgtgcagga tcccgggcga cacggagacg atcgccggga tcacgcgcgt cgcgccctgg
     1981 tccgcgtagg tcgagaccag gtccatcgac aggcgcatgg cgccctcgat ccgatcaacc
     2041 gtgtccgtga tctctgcgtt cgacaggatc ccgttcgcga cgtactggct ctggatgaac
     2101 gtgccgttca ggttcagctc ccagatgaag ctggtgaacg ccgtctcgac ctgtacgagc
     2161 cggttgagcg cgtcctggat gcggtccagc ggctcgcgga tcacctggat caccaggccg
     2221 aagaagctga ccacggagat ggccccgccg gcgaagcgca gctgcggatc gtcgctgaac
     2281 agaagcagcc cgaagccggc ggccaggaag accagaccga cgccggcgaa gaggaagttc
     2341 agcacttgca ggacacgcag cgtctggcgg gcgtcggcca cagcgacggt caggatccgt
     2401 gccgcctcgt cgctgcgctc cttggacggc tcgaggtagt agcgggacag caggtcctgc
     2461 cggctcgcgc gggtgcgctc ctcaccggcc acggcgcgtg ccagcgcgtc caccgccgcc
     2521 cggccgccga ggttgccgag ggtccgcgcg atgttctcgc ggatgtcgac gtcctcctcg
     2581 gactgcagcc ggcgcgccag cagcggcacg gcgtgcaggc gcatctgagc ggtgacgctg
     2641 gcgaaggtcg cctcgtcgcc ctcgtagaag cgggcctcgc tcatctccat gaggtggcgg
     2701 gacagccggc ggtggacctc gacctcgcgc tggcggcgcc tcgactcgag ctcgtgcgcg
     2761 atgcaggcgt cgagctcctc cttgtcgaca ccctcggacg acaggaccac ggccgtcgag
     2821 cgcgctatgc ccgtctcgaa cgacccgagc catttgcgca gccggatgcg gtcgaggtcg
     2881 gggatcgcct ggccgcgcct gaccgcgtcg atcagcgcga ggatgtcgga cggcgtaaag
     2941 ctggccgtga gcacgcgctg gaggtcgccg tccagcggca gggagccggc gaggtggtcg
     3001 agcagcggca gcacactggt gggcttgcgc cgcagcttct cggcagcgct ctccaccagg
     3061 gtggcgcgct ctccgagggc cagccaggtg acccactccc ccaccatggc gcgcggcgca
     3121 tcggcgccgg gcagtcggtc cagcgcctcg agggcctcga gcgccttctc ctcgtccccg
     3181 ccacgcagca ggacgaccag cggcagggtg ctgagcgtgc gccgctgacc tgtgacctcg
     3241 atcagcgccg cgcgggcgct gttctggacg tccgtgtcgg ggtcgctgtc acggaggcgc
     3301 gccaactcct gggccgcctg gtcgatcagg gccgaatcca gcctggccgc gcgcttcccg
     3361 atccagtcgg ccgcctgctg gcggaagcgc cactcaggat cgtgcagcag ctcgatcatc
     3421 aggggccccg cggcggcggc gtgctccctg acctgctcca ggtacggccc ctcttcggcg
     3481 accagcaacg ccctgcccag gccggcccgg tcgagcgtcg tggcacggtg cggcagctgg
     3541 tcgaagaacc cttcgacgcc ctggagcgcg aacagcagct gcgccacccg cgggacccgt
     3601 ggcagcacca gccagagagc gtcgacgatg ccggggcgcg tgtcctccgg gacggcaggc
     3661 agcagctgct cgaggaaggc gatggccgcg tccggccact gcgggggctc ggccagcaac
     3721 cagggcacac cctcgagcgc ctcggcgacc tcctcctggg cgtaggtcag cgtcgacggc
     3781 ggcaggtagc cgccgtaccc ggcgggtgcc tccgaggcca gcacgtcggt cgcccatgcg
     3841 gcgacaccac ccgcgaggtg ctccgcgatc atgctgacgt cgccggcagt ttcgaacgac
     3901 aggcttccga tctgctgcag ccacaggagg atcagcgaca cgcgcacgtc cgagatcgac
     3961 gggtcgtcca taacctcgtt cagcgcgttg gtcagggact ccgcccactc gaggcgctgc
     4021 cgatcgagct cgaaggcctc ggtggggcgg ccgagccggc cggctgcggc cgacaacctc
     4081 aacgccaggg ccggccgatc cggttgctct tcggacagca gcacgatcag ggctccggcc
     4141 cgaaccgacg gcaggtcgta gccgtcgaga agggactcgg ctcgcaccag cgcgtggtcg
     4201 cgctgccggg ctagaaccag cttgaccaat gccgcctgga tggccagctc gcgctcgttg
     4261 accagagccc gccacaagac ctcgttgtcc tggctgctga gcggcagctc cggcctctgc
     4321 aggagactga gcgccccgct cagccggatc gggccctgcg gatcggccag cgccgccacc
     4381 aggccggcca ccgcccgctg caagtccggc tccagctgac cgacagcgcg caccgccgcc
     4441 gcgcgcacgt ccacgtcggc gtcggactcg gccacccgct ggaccagcgg cagcacctga
     4501 tctatcggtg cggacacgct ggcctcccga acggcccgcc gccgctccac ccagctgtcc
//
LOCUS       DATACA010000520         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1999965, whole genome shotgun sequence.
ACCESSION   DATACA010000520 DATACA010000000
VERSION     DATACA010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999965"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VHK28_10500"
     CDS             complement(<1..390)
                     /locus_tag="VHK28_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX2142680.1"
                     /translation="MSTRILDGRTVASRLWRELTDRVAALREGGADEPRLAVVRLGHD
                     GPSAVYAASLGRAARSVGIEPVEVPAPGLGRADLAARIGALNRDPGISGIVVAQPLPA
                     GLDAPSVLDMVDPAKDVDGATALNAGRL"
     gene            complement(387..1043)
                     /locus_tag="VHK28_10505"
     CDS             complement(387..1043)
                     /locus_tag="VHK28_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX2142681.1"
                     /translation="MEGTSNTSLTDLPLRELLARLATRDPVPGGGSAAALAGAMGAAL
                     VSMVTELTIGRPDAADHEDQLLEARSEATSHLAALLDLADEDAAAYHSVVEARRLPRT
                     TDEEQAARLLALNSAMHRAAEAPLRTAQTAARVLDLANGVAPIGNLNAVSDAGVAALL
                     AAASVRGAALNVRINLPYLPAGDPLGTIAPEEVARLEHLADELERSALDIVARRMARP
                     "
     gene            complement(1121..1381)
                     /locus_tag="VHK28_10510"
     CDS             complement(1121..1381)
                     /locus_tag="VHK28_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142682.1"
                     /translation="MALIRIAPAPARVGWDRRHTRPVSVQLADRRLTVTAVEAMRDET
                     AAYPAGRGPRVTYLLATDAGHASLVFDHRQRRWYVEALDTAA"
BASE COUNT          200 a          561 c          583 g          218 t
ORIGIN      
        1 caggcgtccg gcgttgaggg cggttgcgcc gtccacgtcc ttcgccgggt ccaccatgtc
       61 caggaccgat ggagcatcca gtcccgcggg gagcggctgg gcgacgacga tgccggagat
      121 gcccgggtca cggttgaggg cgccgatgcg cgccgcgagg tcggcacggc ccaggccggg
      181 agccggcacc tccacgggct cgatgcccac gctgcgcgcc gcccggccca ggctggctgc
      241 gtacacggcc gacggcccgt cgtggccgag gcgcacgacg gccagtcgag gctcgtccgc
      301 cccgccctcg cgcagcgcgg cgacacgatc ggtcagctct cgccagagtc gcgaagcgac
      361 ggtgcgcccg tccaggatgc gcgtgctcat ggccgcgcca tccttcgggc gacgatatcc
      421 agcgcagagc gctcgagttc gtccgcgagg tgctccagcc gagcgacctc ctcgggggcg
      481 atggtgccca gggggtcgcc ggcgggcagg tacggaaggt tgatgcgcac gttgagcgcc
      541 gccccccgga ccgaggccgc cgccagcagg gcggcgaccc ccgcgtcact caccgcgttc
      601 aggttgccga tgggcgcgac cccgttcgcc aggtcgagga ctcgcgccgc ggtctgcgcc
      661 gttcgcaacg gggcctcggc cgcgcggtgc atggccgagt tcagcgccag cagccgagcc
      721 gcctgctcct cgtcagtggt gcgcggcagc cggcgcgcct cgaccaccga gtgataggcg
      781 gccgcgtcct catcggccag gtccagcaac gccgccaggt ggctggtcgc ctccgacctg
      841 gcctccagca gctggtcctc gtggtcggcc gcgtccggcc ggccgatggt gagctcggtc
      901 accatggaaa ccagcgcggc gcccatggcg ccggccaggg ccgcggcgct gccaccgccg
      961 ggcaccggat ctcgagtcgc cagccgggcg agcagttcgc ggaggggcag gtcggtcagg
     1021 ctggtgttgc tggttccctc cactgggcag gccctcgatg cgcggcgaac cagccgccat
     1081 ccgggctcgc cacctcgatt gtagggggcc tgacgcagga ctaggcggcg gtgtccagcg
     1141 cctccacgta ccagcgtcgc tggcgatggt cgaagaccag cgacgcgtgg ccggcatcgg
     1201 tcgccagcag gtaggtcacg cgcggaccgc ggccggcggg ataggcggcg gtctcgtccc
     1261 gcatggcctc gaccgcggtc acggtcaggc gccggtctgc cagctggacc gatacggggc
     1321 gcgtgtggcg gcgatcccag ccgacgcggg ctggagcggg ggcgatgcgg atgagtgcca
     1381 tggtctggtt ctgactcctt gcacatcgcc tggcagggca ccgaacaggc gttctattga
     1441 tgggtggcca gtgtacgaac atccgttcgg ttctgtcaag acccgtgctg tggcggctcc
     1501 tcatcctccc cacccggagg cggggcgtcg ggccgcatcc cgaagtccgg ccgtcgccgg
     1561 tc
//
LOCUS       DATACA010000521         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_956797, whole genome shotgun sequence.
ACCESSION   DATACA010000521 DATACA010000000
VERSION     DATACA010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956797"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..476)
                     /locus_tag="VHK28_10515"
     CDS             complement(45..476)
                     /locus_tag="VHK28_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142683.1"
                     /translation="MADGAPRRYRLLILLGSLLMIGAGFLPWWRIGGDRVSGVEVPAS
                     SGIGLEGPGLVIFGAAVIALVLLDIGYMRGRWGFLLDAPWAYLLLGLAAGAALGYRFW
                     ELWSVGFLPLPQSSPGLAAAAVGVALVLYGAGIGFTVRQSR"
     gene            complement(484..1038)
                     /gene="hpt"
                     /locus_tag="VHK28_10520"
     CDS             complement(484..1038)
                     /gene="hpt"
                     /locus_tag="VHK28_10520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX2142684.1"
                     /translation="MHDDVEEILLSDDQIRARVAELGAQLAADYADRDPVLVSVLKGS
                     IIFLADLVRATPIPLSVDLMEVSSYGASTESSGQVRILKDLSNAIAGRHVVVVEDIID
                     TGLTLNYLLRYLDDKGPASVRICCLLDKPARRLAEIDIDYRGFTIPDRFVIGYGLDYA
                     ERYRNLPYIGVLKPSVYGAAPPEG"
     gene            complement(1079..1498)
                     /locus_tag="VHK28_10525"
     CDS             complement(1079..1498)
                     /locus_tag="VHK28_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142685.1"
                     /translation="MAEQLVGSPPPRFAHDSEAELARILDYYQVAWRYEPDTFPISWN
                     ADGAVVESFSPDFYLPEVDLYLELTTLRQSLVRKKNRKMRHLRQLYPEVRVKLFYARD
                     FKALMVKYGRLDFLADVQAMNGNGPLDEDDGRLGGED"
     gene            1767..2513
                     /gene="rsmG"
                     /locus_tag="VHK28_10530"
     CDS             1767..2513
                     /gene="rsmG"
                     /locus_tag="VHK28_10530"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX2142686.1"
                     /translation="MSGTDDAARAARTALEPLLADAPAIGSGLPAGALDRLERYVALL
                     LEANARLNLTRVVAPPEVARWHLLDSLAALPLLSETDGPVADLGSGGGLPAVPLAIAR
                     PDLGWTLVESVGKKAHALREIVEALGLEARVVADRAETLGRDPMHRERYAAVTARAVS
                     ALPVLAELALPLLSVGGNLLAWKGPLTDADDEVRRGRVAIGQLGGGPLSLQAALPALG
                     DHTFVVVPKVRPTAARFPRRAGEPARRPLG"
     gene            2786..3508
                     /locus_tag="VHK28_10535"
     CDS             2786..3508
                     /locus_tag="VHK28_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX2142687.1"
                     /translation="MRLAVLSDIHANLAALDAVRADLPAVDETWVLGDIVGYGPQPNE
                     VIRALQEMGARSVMGNHDGAAIGTVDVSWFNPDAARAIDWTSEVVDHNTRAYLASLPE
                     MRREGELTAVHGSPRDPTWEYITGSGVAAENLAAFDTRICLHGHTHVPVIFAADDGQV
                     RIVPATPDLPITVAAGRSLVNPGSVGQPRDGNPAASYMVLDTEAESVQFRRVAYDVEA
                     TQRLMRQHGLPPRLVERLSYGR"
     gene            3615..>3920
                     /locus_tag="VHK28_10540"
     CDS             3615..>3920
                     /locus_tag="VHK28_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEX2142688.1"
                     /translation="MQAEPQEREAAGIAFAFDAESAAELVEQVPADLDFGESALVCVY
                     LGERPTTGWGLTLQSATLVPGEPPELRILARETRPRADARQEVTYPADCAALTRAALP
                     "
BASE COUNT          596 a         1452 c         1339 g          533 t
ORIGIN      
        1 cccagacctg ccgaccggta cggcaggtcg cccagcaccc caactcagcg ggactggcgg
       61 acggtaaagc cgatccccgc gccgtacagc accagcgcca cgcccaccgc ggccgcggcc
      121 aggccaggcg acgactgcgg cagcggcagg aagcccaccg accaaagctc ccagaagcgg
      181 tagccgagcg ccgcgccagc cgccaggccc agcagcaggt acgcccacgg agcgtcgagc
      241 aggaagcccc agcgcccgcg catgtaaccg atgtccagca gcaccagcgc gatcaccgcc
      301 gcgccgaaga tcaccaggcc gggcccttcg agcccgatcc ccgacgaggc gggaacctcg
      361 acgccgctca cccggtcgcc gccgattcgc caccacggca ggaacccggc gccgatcatc
      421 agcaggctgc ccagcaggat cagcagccgg tagcggcgcg gcgcaccgtc ggccatgacg
      481 acgtcagccc tccggcgggg ctgctccgta gacggagggc ttcagcacgc cgatgtacgg
      541 caggttgcgg tagcgctccg cgtagtccag cccgtagccg atcacgaagc ggtccgggat
      601 ggtgaagccg cggtagtcga tgtcgatctc cgccagccgc cgcgccggct tgtccagcag
      661 gcagcagatg cggaccgagg ccgggccctt gtcgtccagg tagcggagca ggtagttcag
      721 ggtcaggccg gtgtcgatga tgtcctcgac caccaccacg tgacgcccgg cgatcgcgtt
      781 cgacaggtcc ttcaggatgc gcacctgccc ggatgactcg gtcgaggccc cgtaggacga
      841 gacctccatg aggtccaccg acagcgggat gggcgtggcc cgcaccaggt cggccaggaa
      901 gatgatcgag cccttcagca cgctgaccaa gaccgggtcg cggtccgcgt agtcggcggc
      961 cagctgcgcg ccgagctcgg cgacgcgcgc acggatctgg tcgtcggaga gcaggatctc
     1021 ctcgacgtcg tcgtggatgg acgtggatgc ggttgccgcg ccggggttca tcggtccatc
     1081 agtcctctcc gccaaggcgc ccgtcgtcct cgtccagcgg gccgttgccg ttcatggcct
     1141 gcacatcggc caggaagtcc aggcgtccgt acttgaccat cagcgccttg aagtcacggg
     1201 cgtagaagag cttcacccgg acctccgggt agagctgtcg gagatgccgc atcttccggt
     1261 tcttcttgcg caccagcgac tgcctgaggg tcgtcagctc caggtagagg tcgacctcgg
     1321 gcagatagaa atccggcgaa aacgactcga cgaccgcgcc gtcggcgttc caggagatgg
     1381 ggaaggtgtc tggttcgtat cgccatgcca cctggtagta gtcgaggatc cgcgccagct
     1441 ccgcctcgct gtcgtgcgcg aagcgcggtg gtgggctgcc aaccagctgt tccgccacct
     1501 gctcctcaga tcctacgcgc ctgggtcgcc ggggtcatcg gaatcgagta tatcccgcgg
     1561 ggacttatcc acatcgtggt ggataacgcg ccgaatcgtg gaaaagtggg ttgcccgtcc
     1621 acaccccgcg acgctatggt tgccgtcctg cttccccggt ggccgagtac ggtaaccggg
     1681 ctggcgcggg gccgatgcga aagccggcca tccctaccgc gcctgacccg ggaagaccga
     1741 aacgccatcc gaggaggcat tttcatttgt ccgggaccga tgacgccgcc cgcgcggcgc
     1801 ggacggcgct cgaacccctc ctggccgacg cccccgccat cggctcgggg ctgcccgccg
     1861 gcgccctgga ccgcctcgag cgctacgtgg cgctcctgct ggaggccaac gcccggctga
     1921 acctgacccg ggtggtggcg ccccccgagg tcgcgcgatg gcacctgctc gactccttgg
     1981 ccgcccttcc cctgctgtcc gagaccgatg gccccgtcgc cgacctcggc tccggcggcg
     2041 ggctgcccgc cgtcccgctg gcgatcgccc gccccgacct gggctggacg ctggtcgagt
     2101 cggtcggcaa gaaggcccat gccctgcgcg agatcgtgga ggcactgggc ctggaagcac
     2161 gagtggtggc ggaccgtgcc gagacgctcg gccgcgaccc gatgcaccgt gagcggtacg
     2221 cggcggtgac cgcccgagcc gtctccgcgc tgcccgtgct ggccgagctt gccctgccgc
     2281 tgctgtcggt tggcggcaac ctgctggcct ggaagggccc gctgaccgat gccgacgacg
     2341 aggtccgccg cggccgcgtg gcgatcgggc agctgggagg cggcccgctg tcgctgcagg
     2401 ccgcgctgcc ggcgctcggc gaccacacct tcgtggtggt ccccaaggtt cggcccacgg
     2461 cggcccgctt cccgcggcgt gccggggagc cagcccgccg cccgctcggc tgacgttcct
     2521 gccacaaacc gccgggcgcc ggcctcatcc acgtacgggg accggcttcc tgccatggac
     2581 agccgcgcgg ccgcctaagc cagattgccc acgctcagcg accgaccagc cacgctcacc
     2641 gaacctggct acgcccgcca cctgccgcac tctgacaagg gaaccagtga cgatgcagca
     2701 cctaagacag cccggcgacg ggttttggca ggaaaccagc ggaacccggg ccgcaaaacc
     2761 ctcatcggta tactcgcaac cgcccatgcg ccttgcggtc ctgtcggaca tccatgccaa
     2821 cctcgctgcg ctcgacgcgg tgcgcgccga cctgcccgcc gtcgacgaga cgtgggtgct
     2881 gggcgacatc gtcggctacg gcccgcagcc caacgaggtg atccgcgcgc tgcaggagat
     2941 gggcgcccgc agcgtgatgg gcaaccacga cggcgcggcc atcggcaccg tcgacgtcag
     3001 ctggttcaac ccggacgcgg cgcgcgccat cgactggacc agcgaggtgg tggaccacaa
     3061 cacgcgcgcc tacctggcca gcctgcccga gatgcggcgc gaaggcgagc tgacggcggt
     3121 gcacggctcg ccccgcgacc cgacctggga gtacatcacc ggctccggcg tggccgccga
     3181 gaacctggcc gccttcgaca cccgcatctg cctccacggc cacacccacg tcccggtcat
     3241 cttcgccgcg gacgatggcc aggtgcgcat cgtgccggcc acgccggacc tgccaatcac
     3301 ggtggccgcc ggccgctccc tggtcaatcc gggcagcgtc ggccagccgc gcgacgggaa
     3361 cccggccgcg tcctacatgg tcctggacac cgaggcggag tcggtgcagt tccggcgcgt
     3421 tgcgtacgac gtggaggcca cccagcgcct catgcgccag cacggcctcc cgccacggct
     3481 ggtggagcgc ctgagttacg ggcgctagac acctggcgcc gcgcgcggcg ctggccgtcc
     3541 tgctgctggt cgctggctgt gggccgatgg cgtcgccgtc tccatcggtc cagccagggc
     3601 tgacgctacg tgacgtgcag gccgagccgc aggagcgcga ggccgcgggg atcgcgttcg
     3661 cattcgacgc agaatccgcc gcggagctgg tggagcaggt gccggccgac ctggacttcg
     3721 gagagagcgc cctggtctgc gtctacctcg gggagcggcc gaccaccggc tggggcctga
     3781 ccctgcagtc cgccaccctg gtcccaggcg agcccccgga gctgcgcatc ctggcccgcg
     3841 agacgcggcc gcgggcggac gcccggcagg aggtcaccta cccggccgac tgcgccgcgc
     3901 tgacccgggc cgcgctgccg
//
LOCUS       DATACA010000522         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2161135, whole genome shotgun sequence.
ACCESSION   DATACA010000522 DATACA010000000
VERSION     DATACA010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161135"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VHK28_10545"
     CDS             <1..753
                     /locus_tag="VHK28_10545"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX2142689.1"
                     /translation="IPATTALDAGRLVLGADADMLARLADPSNRPAKAASRDLGPLLA
                     ARRMASTTVSATMRIAHLAGVRVFATGGIGGVHRGAASSFDVSSDIDELAATPVAVVC
                     SGAKSILDLPATLELLETRRVPVVGIGVDELPAFYAASSGLPLAHRADSPDEAAAMVA
                     AHLALPGSGGVLLVQPPPPEVALGASEVGGWIAAALGDAEGSGIRGGAVTPFVLARVA
                     EASGGRSLVANIGLIINNARMAGEIAVALFRR"
     gene            808..1344
                     /locus_tag="VHK28_10550"
     CDS             808..1344
                     /locus_tag="VHK28_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142690.1"
                     /translation="MRIYEGSPRQDWEEVLRAIGAFADREQLKELLFLELDDGFLLQG
                     LALPAGGADSDSFGALSKRMYELVDEQVAQLMDEAAAQRGSAEVSVPQPGLTNYYEQA
                     LRIVGAWVDREHARDLFFFEQDGSFVLRVLHATAGGAIGHQLAEFTKDEIVAMIEAAP
                     QQRNAEPEGAGAPTGEAS"
     gene            1344..1496
                     /locus_tag="VHK28_10555"
     CDS             1344..1496
                     /locus_tag="VHK28_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142691.1"
                     /translation="MKALKVLLISIAALVAAMIVIPLTILAGLFVWLKLTEEADEDDL
                     EIEDTV"
     gene            complement(1554..2384)
                     /locus_tag="VHK28_10560"
     CDS             complement(1554..2384)
                     /locus_tag="VHK28_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX2142692.1"
                     /translation="MSRSFAPIAWLKLIHPAPAVAVMLLAAVLGAILLSQAGIALGER
                     LALTVAAVAGSQVFTGAVNDLVDRGRDAAAGRLDKPLATGAATPEAAVWVASAGLAVQ
                     LAASLRLGVLPLALGVAATLSALAYNLWLSRTPLSVVPYVVSFSLLPLWIAAGVGVPL
                     ERVLPGVPLVGPFAAAAHLANLGRDWDADAELGSRSLAQVLGRSTAHRMAYGLALAVG
                     GGIGTAFLLGGQITAPTLLLGLLGLAAVAQGYASSRRLWYGMLLAAVCWTAAWAISTG
                     "
     gene            2442..2630
                     /locus_tag="VHK28_10565"
     CDS             2442..2630
                     /locus_tag="VHK28_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142693.1"
                     /translation="MNASNERGMRPDRSIRGLIRALEARLQRQRELLANPRLELAAAR
                     QMARTQRRLAVLRRNQPH"
     gene            2837..>3681
                     /locus_tag="VHK28_10570"
     CDS             2837..>3681
                     /locus_tag="VHK28_10570"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX2142694.1"
                     /translation="MPKYVFVTGGVTSSLGKGITAASIGRILKARGIPVSILKLDPYI
                     NIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLSQASNVTTGRIYQSVIAKE
                     RRGDYLGGTVQVIPHITNEIKERIARVARDGTGSVAASGLAAHGVVIVEVGGTVGDIE
                     SLPFLEAIRQMRKDVGRRNVLYVHVTWLPQIGATGELKTKPTQHSVSTLRGIGIQPDV
                     IVLRSDEPIDDEILDKVALFTDVDVHAVIPLRTAKSIYEVPLLLERAGLGKLITRDLE
                     IPASEP"
BASE COUNT          587 a         1327 c         1257 g          510 t
ORIGIN      
        1 ataccggcca ccacggcgct cgacgccggg cggctggtgc tgggcgcgga cgccgacatg
       61 ctcgcccgcc tggcggaccc gtcgaaccgc ccggccaagg ctgcctcgcg tgacctcgga
      121 ccgctgctcg ccgcgcgtcg catggcgtcg acgaccgtct cggccacgat gcgcatcgct
      181 catctcgccg gggtccgcgt cttcgccact ggtggcatcg gcggcgtgca ccggggagcc
      241 gccagctcct tcgacgtgtc gtccgacatc gacgagctgg ccgccacgcc ggtggcggtg
      301 gtctgctcgg gggccaagtc gatcctcgac ctgcccgcca ccctcgagct gctcgagacg
      361 cgcagggttc cggtggtcgg catcggggtc gatgagctgc ccgccttcta cgccgcctcg
      421 tcaggactgc cgctggcgca ccgcgccgac tcaccggacg aagcggcagc catggtggcc
      481 gcccacctgg cgttgcccgg ttcgggcggc gtcctcctgg tccagccgcc accgccggag
      541 gttgcgctcg gggcgtccga ggtgggcggc tggatcgcgg cggcgctcgg ggacgccgaa
      601 ggctccggca tccgcggcgg tgcggtaacg cctttcgtcc tggcgcgggt ggccgaggcg
      661 tccggcgggc ggagcctggt ggccaacatc gggctcatca tcaacaacgc gcgaatggcg
      721 ggcgagatcg cggtggccct gtttcggcgc tgacgtcggt ggagtatgct gcccgccttc
      781 cccacggcgc ggcggtagga gggtggcatg cggatttacg agggcagccc gcggcaggac
      841 tgggaggaag tcctgcgcgc catcggtgca tttgccgatc gcgagcagct caaggagctt
      901 ctcttcctgg agctggacga tggcttcctg ttgcagggac tggccctccc ggccggcggt
      961 gccgactccg actcgttcgg cgcgctctcc aagcgcatgt acgagctggt tgacgagcag
     1021 gtcgcccagc tcatggacga ggcggccgcc cagcgcggct cggccgaggt ctcggttccg
     1081 cagccgggcc tgaccaacta ctacgagcag gcgctgcgca tcgtcggggc ctgggtcgac
     1141 cgcgagcacg cgcgcgacct gttcttcttc gagcaggacg ggtcgttcgt cctccgcgtc
     1201 ctgcacgcca ccgcgggcgg cgccatcggt caccaattgg ccgagttcac gaaagacgag
     1261 attgtggcta tgatcgaggc cgcacctcag cagcggaacg cggagccgga aggtgccggc
     1321 gcgcccacag gggaggcctc gtgatgaagg cgttgaaagt cctgctcatc agcatcgccg
     1381 cactcgtggc ggcaatgatc gtgatcccgc tcaccatcct ggccgggctc ttcgtgtggc
     1441 tcaagctcac cgaggaggcc gacgaggacg acctggagat cgaggacacg gtctagctgg
     1501 accgatgcgg gcgccggctc ggcgcccgcc ttcgactcac cggccggcat ttgctagccg
     1561 gtgctgatgg cccatgccgc cgtccagcac acggccgcca gcagcattcc gtaccacagg
     1621 cgccgtgagc tcgcgtagcc ctgcgccacg gccgccaacc ccagcagtcc cagcaacagc
     1681 gtcggggcgg tgatctgtcc gccgagcagg aacgccgtgc cgatgccacc accgaccgcc
     1741 agcgccagcc cgtaggccat ccggtgcgca gtgctgcggc cgagcacctg cgccaggctg
     1801 cgtgagccga gctccgcgtc cgcgtcccag tcgcggccga ggttggccag gtgcgctgcc
     1861 gccgcgaagg gaccgaccaa cggcacgccg ggcagcacgc gctctaacgg cacgccgacg
     1921 ccggccgcga tccacagcgg cagcaggctg aagctcacga cgtacggcac caccgacagc
     1981 ggcgtgcgcg aaagccacag gttgtacgcc agcgctgaga gcgttgccgc gacacccagg
     2041 gccaggggca gcactcccag ccgcagcgag gccgcgagct gcacggccag tccggcggat
     2101 gccacccaga ccgccgcctc cggggtcgcg gcgccggtgg ccaggggctt gtccaggcgt
     2161 cctgcggccg cgtcacggcc gcggtcgacc aggtcgttga cggcgcccgt gaacacctgg
     2221 gagccggcca ccgccgccac cgtcagcgcc aaccgctcgc cgagcgcgat cccggcctgg
     2281 gacagcagga tggcccccag caccgcggcc aggagcatga cggccacggc gggtgctgga
     2341 tggatcagct tcagccaggc gatcggcgcg aaggagcggc tcacgcgggc atgctagacc
     2401 gtgcccgccg cgtggcacgc ttgctgcata tcagtcatcc catgaacgca tcgaacgaac
     2461 gaggcatgcg acctgatcgg tccattcgcg ggctgatccg cgcgctcgag gcacgcctgc
     2521 agcggcagcg cgaactgctc gccaatccgc gactggagct tgccgccgcg cgccagatgg
     2581 cccgcacgca gcgccgcctg gcggtcctcc gccgaaacca gccccactag cggcctgttc
     2641 ccgcgccgaa accggcattt gggcctggtc ctgcgccgta tgcactgaca aataggtcac
     2701 gcaacccgtc cggcctccgc cacgcgacct atgagcgcat acatcgatca ggcggaagcg
     2761 cgagcggccc aaggccgcct gtggaggcag ccaattgcgc tagaatcgga aaaaccccgg
     2821 aggatcagag aaaccgatgc cgaagtatgt cttcgtcacg ggcggcgtca ccagctcgct
     2881 cggcaaaggc atcaccgccg cctccatcgg gcgcatcctc aaggcccgcg gcatcccggt
     2941 ctccatcctc aagctggacc cctacatcaa catcgacccc ggcaccatgt cgccgtacca
     3001 gcacggcgag gtgttcgtca ccgatgacgg tgcggagacg gacctggacc tggggcacta
     3061 cgagcgcttc atcgacgaga acctgtccca ggccagcaac gtcaccaccg ggcgcatcta
     3121 ccagagcgtg atcgccaagg agcgccgcgg cgactacctg ggcggcacgg tgcaggtcat
     3181 cccgcacatc accaacgaga tcaaggagcg catcgcgcgc gtggcgcgcg acgggaccgg
     3241 cagcgtggcc gccagtgggc tggcggcgca tggcgtggtc atcgtggagg tgggtggcac
     3301 ggtcggcgac atcgagtcgc tgcccttcct ggaggccatt cgccagatgc gcaaggacgt
     3361 cggacgccgc aacgtgctct acgtgcacgt cacctggctg ccgcagatcg gcgccaccgg
     3421 cgagctgaag accaagccca cccagcacag cgtcagcacg ctgcgcggca tcggcatcca
     3481 gccggacgtg atcgtcctgc gcagcgacga gccgatcgac gacgagatcc tggacaaggt
     3541 cgcgctcttc accgacgtgg acgtccacgc cgtgatcccg ctgcgcactg ccaagagcat
     3601 ctacgaggtg ccgctgctcc tggagcgcgc cggccttggc aagctgatca cccgcgatct
     3661 ggagatcccg gccagcgagc c
//
LOCUS       DATACA010000523         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2241911, whole genome shotgun sequence.
ACCESSION   DATACA010000523 DATACA010000000
VERSION     DATACA010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241911"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VHK28_10575"
     CDS             complement(<1..798)
                     /locus_tag="VHK28_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2142695.1"
                     /translation="MRRDVLWLTGVALVVRVAAAALVDYPPYTDPAYYLLVADRVADG
                     FGFTVPVLWSFLEVGGRMPAEASLPAASNGHWMPLTSLVAVPFIWVLEPAIGAWRAAQ
                     VPFVLLSTALVPFTYLVGWELWRSRRAALGGALLAVLAGPFLVYYPQVNNIAVFGALG
                     AGAVWCAVRAVRADRPGVWLVGSAALAGLATLARVDGLLLIVPVAVAWWVRRESWAWA
                     VAAGVAFVAVMAPWLLRNLAVFGSAFPSADGHTLWITTYNGQFSIAAD"
     gene            complement(795..2327)
                     /locus_tag="VHK28_10580"
     CDS             complement(795..2327)
                     /locus_tag="VHK28_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142696.1"
                     /translation="MSMGATRVDRGVAAATAAVVGFFVAAVLPRATWPLIDGDVWWHI
                     RAGEEVLRSGSVPRTDTWSIAGLGREWTSQDWGANVILALGENAGPWGYTALSFLFGA
                     FTVLAFWILWRAVAVRYPGAGWASRVVWLSIGLTLAGPVMGVRVQVLDLLLATVVIWT
                     CWRYLVDPRRRWLVILPLVAVLWANLHAGWVLLFLLGGAVLVGEAVDRLLRRRLEGPP
                     SLTWVQLRDLGLALVVSAGALVLNPNGIDLYAYPFYTVGITALNRYVMEWFPASLDSL
                     FGWLLLGFVVVGVLPTVVFGRGRLRTADALILVGVTVMAWQAIRFLLIAGPIGAAVAA
                     VVLSPVISSTRIGQRWSPMLARLARPRSGRLGTVNRVLVGLLVVIGLGVAFARVNPAA
                     QAVEIARGLPVEAVRWMDEHDPGDRIFNRYEWGGYIGQHRPHELVFMDGRADVYGDEL
                     LQMYVSVIGVQGDPQEVFDRYEIDHVVIPPDWDLAAWLDDSSAWQRAYTDEVAAIWVR
                     SS"
     gene            2417..>3090
                     /locus_tag="VHK28_10585"
     CDS             2417..>3090
                     /locus_tag="VHK28_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX2142697.1"
                     /translation="MVRSSRARSSFAPSRVALALVLLVYGLLGLALIAFLAPSTFIGA
                     DLAVYQRAGRDLLTEGNPYASAAELPYVFQYRYPPLLAMLMPVLGWPPLWYGLMAAAT
                     LGTFLVGVRVGGAAYLLPVVVLGGAWGQVLLNGNVQPLLMLLLAVVPLFRRAGAVSLA
                     VATMIKLHPVLGVVWYLGRRDWVALGWYAAAMAALTLVQAPWLPHFVDFYLEQELASP
                     FGQQGFG"
BASE COUNT          525 a         1169 c         1019 g          377 t
ORIGIN      
        1 atcggccgcg atcgagaact gcccgttgta ggtcgtgatc cacaacgtgt gaccgtccgc
       61 ggacgggaac gctgacccga acacggccag gttgcgcagg agccagggcg ccatgaccgc
      121 cacgaaggcg acccccgctg ccaccgccca ggcccacgac tcgcggcgca cccaccacgc
      181 aaccgccacc ggcacgatca gcagcagccc gtccacgcgt gccagcgtgg ccaaccccgc
      241 cagcgcagcg gacccgacca gccacacgcc gggccggtcg gcccgcaccg ctcgcaccgc
      301 gcaccacacg gcacccgccc ccagcgcgcc gaacaccgcg atgttgttga cctgcgggta
      361 gtagacgagg aacggcccgg ccagcacggc cagcagggcg ccgcccaagg ccgcgcgccg
      421 ggaccgccac agctcccagc cgaccaggta ggtgaacggc accagcgcgg tcgacagcag
      481 gacgaacggc acctgcgccg cacgccaggc cccgatcgcc ggctccaaca cccagatgaa
      541 cggcaccgcc accagcgagg tcagcggcat ccagtgaccg ttggacgccg ccggcagcga
      601 ggcctccgcc ggcatgcgcc ctcccacctc caggaaggac cacagcacgg gaacggtgaa
      661 gccgaaccca tcggccacgc ggtcggccac caataagtag taggcgggat cggtatacgg
      721 cgggtagtcc accaacgcgg ccgccgccac ccgcacaacc aaggcaaccc cggtcagcca
      781 caagacgtcc cgcctcacga ggagcgcacc cagatggcgg ccacctcatc ggtgtatgcc
      841 cgttgccagg cggaggagtc gtccagccag gccgcaaggt cccagtcggg cgggatcacc
      901 acgtggtcga tctcgtatcg gtcgaacacc tcctgcgggt cgccctgcac cccgatcacg
      961 gagacgtaca tctggagcag ctcgtcgccg tagacatcgg cccggccgtc catgaagacc
     1021 agctcatgtg gccggtgctg accgatgtac ccgccccact catacctgtt gaagatgcgg
     1081 tcaccggggt cgtgctcgtc catccagcgc acggcctcca ccggcaagcc gcgcgcgatc
     1141 tcgactgcct gcgcagccgg gttgacccgc gcgaaggcca cccccagacc gatgaccacc
     1201 agcagcccga ccagcacccg gttcacggtt cccagcctgc ccgagcgggg ccgggccaat
     1261 cgggccagca tcggggacca ccgctgcccg atgcgagtgc tcgagatcac cggcgagagc
     1321 accacggctg ccaccgcggc accgatgggc ccggcgatca gcaggaagcg gatggcctgc
     1381 caggccatga ccgtcacccc aacgaggatc agcgcgtccg ccgtccgcag ccggccgcgg
     1441 ccgaagacca cggtgggcag cacgcccacc accacgaatc ccagcagcag ccagccgaag
     1501 agggagtcga ggctggccgg gaaccactcc atgacgtatc ggttgagggc ggtaatgccc
     1561 accgtgtaga acggataggc gtagaggtcg atgccgttcg ggttgagcac cagcgcgccg
     1621 gcggacacga ccagcgccag ccccaggtca cgcagctgta cccaggtcag cgacggcggc
     1681 ccctccagcc ggcgtcgcag gagccgatcc acggcctcgc ccaccaacac ggcaccgccc
     1741 agcaggaaga ggagcaccca gccggcatgc aggttggccc acagcacggc aaccagcggc
     1801 aggatgacca gccagcgtcg tcgcgggtcg accaggtagc gccagcaggt ccatatcacc
     1861 accgtggcca gcagcaggtc cagcacctgg acgcgcacgc ccatgaccgg tccggccagc
     1921 gtgagaccga tggacagcca gaccacccgg ctggcccatc cggcaccggg gtagcgcacg
     1981 gcgacggcgc gccacaggat ccagaaggcg agcacggtga acgcgccgaa caggaaggag
     2041 agcgccgtgt agccccacgg cccggcgttc tcgcccagcg cgaggatgac gttggcaccc
     2101 caatcctggc tggtccattc ccgaccgagg ccggcgatgc tccaggtgtc ggtgcgcggc
     2161 acgctgccgc tgcgcagcac ctcctcgcca gcgcggatgt gccaccacac gtcgccgtcg
     2221 atcagcggcc aggtggcgcg aggcagcacg gcggccacga agaagccgac caccgcggcg
     2281 gtggcggccg ccacgccacg gtcgacgcgc gttgcaccca tggacatgcg cgggagtcta
     2341 gggccgcatg ggtgcgtccc gtcaccaccc cgaagtaccg ccggtcgggc gtgggacgcg
     2401 tgctagcctc gccgccgtgg tccgatccag ccgcgcccgg tccagcttcg ctccctcgcg
     2461 cgtcgcgctc gcgctcgtgc tgctcgtcta cggtctgctg ggcctggcgc taatcgcgtt
     2521 cctggcgccc agcaccttca tcggtgccga cctggccgtc taccagcgtg cggggcgtga
     2581 cctgctgacc gaaggcaatc cgtacgccag cgccgccgag ctgccgtacg tcttccagta
     2641 ccggtaccca cccctgctgg ccatgctcat gcccgtgctt ggctggccgc cgctctggta
     2701 cggactgatg gcggcagcaa ccctgggcac cttcctggtc ggggtgcggg tgggtggagc
     2761 cgcctacctg ctgccggtgg tcgtgctggg cggggcgtgg ggccaggtgc tgctgaacgg
     2821 caacgtgcag ccgctgctga tgctgctcct ggccgtggtc ccgctcttcc ggcgcgcggg
     2881 cgccgtgtcg ctggcggtcg ccaccatgat caagctgcat cccgtcctgg gcgtggtctg
     2941 gtacctcggc cgccgcgatt gggtggcgct cggctggtac gccgcggcca tggcggcgct
     3001 caccctggtc caggcgccgt ggctgccgca cttcgtggac ttctatctcg agcaggagct
     3061 cgcctcaccg ttcgggcagc agggattcgg
//
LOCUS       DATACA010000524         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1679029, whole genome shotgun sequence.
ACCESSION   DATACA010000524 DATACA010000000
VERSION     DATACA010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679029"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VHK28_10590"
     CDS             <1..718
                     /locus_tag="VHK28_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142698.1"
                     /translation="PLAGPALAALVTLVTPTGHAGAFPLRGCALTVTSLDASGTALDT
                     ARDGEATGNQAISAEPEGGTADDPLRIAWNGALQWAAAGDEEPIESGQLRLAVFGIPL
                     PAVDLASGASMDLGEAVPFRYTGLYYLSGQLRSGERVCAGSGWVLVVGEPAGTVPFLV
                     ALLLAVLGIVLLATAVRGEWLAAALGGPLLGLGGAVLMVLYATLPFGEATPWMVIGVG
                     MLLGAVAGYGGRRRRARPAA"
     gene            complement(734..1063)
                     /locus_tag="VHK28_10595"
     CDS             complement(734..1063)
                     /locus_tag="VHK28_10595"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142699.1"
                     /translation="MYYDLLLDEQRARRPDLAIIRIERLYPFPTEELGDVLARYPKMQ
                     RIAWVQEEPRNMGARKFVLPKIRHLVPFRIPMADISRPERSRPAEGYPAAHAAEQGRI
                     VREALGG"
     gene            complement(1064..4405)
                     /locus_tag="VHK28_10600"
     CDS             complement(1064..4405)
                     /locus_tag="VHK28_10600"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEX2142700.1"
                     /translation="MPPTERDDPSSLAIALDSVNAGYVAERYEQYRRDPESVEPEWRS
                     LFDGGAGGFEPVARPAPATAASSDGNGSGGPADGGTGTAEQPPAEAPEAEAPRAEPPP
                     GASPIRGPAASLARNMAASLEVPTATSFRDVEVRTLEARRRELNGQLAPTKVSFTHLI
                     GWAIVRAASDQPSMTHYFTQVDGAPHRVDPGGVNLGLAVDVERPDGSRFLVVPVIRGA
                     DGMDFAEFLDRYEELVQRSRANRLSPDDFAGATITLTNPGTLGTTASVPRLMPGQGTI
                     VATGAIRDVGERRVMTISSTYDHRIIQGAESGTFLRRIDELLDGQDGFYEEVFTAKGA
                     SLGTDVAAPAAEPAPPPASSAQFAPDRAGLKAIAAAVALVRAYRSFGHLAAQLDPLGS
                     QPPGDPALDPAGLGLTPEVMAAIPADLLRIACPGETLAEALPNLVATYCGSIAYEVEH
                     LASHEERMWLREAIESGMHRRPLSADERRALLMRLTEVEGLERFLHRAYLGQKRFSIE
                     GLDAMVPMIDTVLTDAAAAGTRDVVIGMAHRGRLNVLAHVVGVPYEAILAEFEAGRGG
                     TDARTPKGGSDDVKYHLGAEGAYRTRDGHEVRITVSPNPSHLEAVDPVVEGRARAQQS
                     DRTGREVEVDPDRTLPLLIHGDAAFAAQGVVAETLNLARLPGYSTGGTVHIIANNQVG
                     FTTGPREGRSTAYSSDLAKGYDVPIIHVNADDAEACVAAARLAMAYRERFHGDVVIDL
                     VGYRRYGHNEGDEPAYTQPAMYRAIAEHPSVRDLYHRQLVTDGVLTSEQGDEQAREMA
                     RDLATRQAAVRKQHAEPQLDRGSEALSDGGASEPETAVPADRLARLNAGIYRWPDGFA
                     VHPKLRKQLEGRQAALEGDDARVDWSQAEALAFASLLADGTPVRLTGQDTVRGTFSQR
                     HATLFDAGNGEAYTPLAHLEEARASFELHNSPLSEYACLGFEYGYAVTAPEALVLWEA
                     QYGDFVNGAEIIIDQFLIAGLAKWRQTSRLTLLLPHGYEGSGPEHSSARLERFLALGA
                     EGNIRVVYPTTPAQYFHLLRRQGLHGDLRPLVVMTPKSLLRLPEASSPTAELSRGRFS
                     PVLDDPSTASEEAAAAVRHVILCGG"
     gene            complement(4438..>4836)
                     /locus_tag="VHK28_10605"
     CDS             complement(4438..>4836)
                     /locus_tag="VHK28_10605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142701.1"
                     /translation="GLIAFDGRQPVGWVSVGPRVEFGRILRSPVTRLSPEERSDPSVW
                     SVVCFFIPRTNRGRGIGRFLLDAAVDHARRSGASRVEGYPVDTAGRRVASASVFIGTR
                     DLFERAGFERLAEHWKGRPIMTRVLEQGVR"
BASE COUNT          641 a         1675 c         1799 g          721 t
ORIGIN      
        1 tccgctggcc ggacccgccc tggctgcgct cgtcacgctc gtcacaccga ccgggcacgc
       61 cggcgccttc ccgctcaggg gctgcgccct gacggtgacc tcgctggatg cgtccggaac
      121 cgccctcgac acggctcgtg acggcgaggc gaccggcaat caggccatct ccgccgagcc
      181 ggagggaggg acggcagacg atcctctgcg catcgcctgg aacggtgctc tgcagtgggc
      241 cgccgccggt gacgaggagc cgatcgagag cggccagctg cgcctcgccg tcttcgggat
      301 cccgctgcct gctgtcgacc tggcatccgg cgcgtccatg gacctggggg aggcagtccc
      361 gttccgatac accgggctct actacctcag cggccagctg cgctccggcg agcgcgtctg
      421 cgcggggagc ggctgggtgc tggtggtcgg cgagccggcc ggcacggtgc cgttcctggt
      481 tgcgctgctg ctggctgtgc tggggatcgt tctgctggca acggccgttc gcggggagtg
      541 gctcgcggcg gcgttgggcg ggccgctgct ggggttgggc ggcgcggtcc tgatggtgct
      601 gtacgccacc ctgccgttcg gcgaggccac gccgtggatg gtcatcggcg ttggcatgct
      661 gctgggtgcg gtggcggggt acggtgggcg ccgcaggcga gcgcggccgg ccgcctgagc
      721 gaccggcacg gagtcagcct ccgagtgcct cgcggacgat gcgcccctgc tccgctgcgt
      781 gggccgccgg gtagccctcg gcgggccgcg accgttccgg ccggctgatg tcagccatcg
      841 ggatgcggaa aggcaccagg tggcggatct tgggcagcac gaacttgcgc gcgcccatgt
      901 tccgtggctc ctcctggacc caggcgatgc gctgcatctt cgggtagcgg gccagcacgt
      961 cgcccaactc ctcggtgggg aacgggtaga gccgctcgat gcggatgatc gccaggtccg
     1021 gccgccgggc gcgctgctcg tcgagcagca ggtcgtagta cacctagccg ccgcacagga
     1081 tcacgtggcg gacggcggcg gcagcctcct ccgaggcggt ggacgggtcg tccagcacgg
     1141 gtgagaagcg gcctctgctc agctcggccg tcggcgagga tgcctccggc aggcgcagca
     1201 gcgacttggg ggtcatcacc accagcggcc ggaggtcgcc gtgcaggccc tgccggcgaa
     1261 ggaggtggaa gtactgggcc ggcgtggtcg ggtacacgac ccggatgttt ccctcggcgc
     1321 cgagggccag gaagcgctcc aggcgagcgg acgagtgctc cggtcccgag ccctcgtagc
     1381 catgcggcag gagcagggtc aggcgcgagg tctggcgcca cttggccagc ccggcgatca
     1441 ggaactggtc gatgatgatc tcagcgccgt tgacgaagtc gccgtactgc gcctcccaca
     1501 ggaccagcgc ctcgggtgcg gtgaccgcgt atccgtactc gaagcccagg cacgcgtact
     1561 ccgatagcgg gctgttgtgg agctcgaagc tggcccgcgc ctcctccagg tgggcgagag
     1621 gcgtgtacgc ctcgccgttg ccggcgtcga acagggtcgc gtgccgctgg ctgaaggtgc
     1681 cgcgcacggt gtcctgcccg gtcagccgga ccggcgtccc gtcggccagc agcgaggcga
     1741 aggccagcgc ctcggcctgc gaccagtcga cgcgtgcatc gtcgccctcg agagccgcct
     1801 gccgtccctc gagctgcttg cgcagcttgg gatggacggc aaacccatcc ggccagcggt
     1861 agatcccggc gttcagccgc gccaggcggt cggctggcac ggccgtctcc ggctccgatg
     1921 ccccaccatc ggacagcgcc tcggaaccgc ggtcgagctg gggctcggcg tgctgcttgc
     1981 ggacggccgc ctggcgcgtg gccaggtcgc gagccatctc gcgggcctgc tcgtcgccct
     2041 gctcggaggt gaggacgcca tcggtcacca gctggcggtg gtagagatcg cgaacggacg
     2101 ggtgctcggc gatggcgcgg tacatggccg gctgggtgta tgccggctcg tcgccctcgt
     2161 tgtggccgta gcgtcggtac ccgaccaggt cgatgaccac gtcgccgtgg aagcgctccc
     2221 ggtaggccat cgccaaccga gcggcggcca cgcaggcctc cgcgtcgtcg gcgttgacgt
     2281 ggatgatcgg cacgtcatag cccttggcca ggtcggacga gtaggcggtc gagcggccct
     2341 cgcgcgggcc ggtggtgaag ccgacctggt tgttggcgat gatgtggacc gttccgccgg
     2401 tcgagtagcc gggcagccgc gccagattga gagtctcggc caccacgccc tgggcggcga
     2461 aggcggcgtc gccgtggatc agcagcggca gggtgcgatc gggatcgacc tccacctcgc
     2521 gtccggtgcg atccgactgc tgtgcgcggg cacgcccctc gaccaccgga tcgaccgcct
     2581 ccaggtggct ggggttgggc gagacggtga ttcgcacctc gtgcccgtcg cgggtgcggt
     2641 aggcgccctc cgcgccgagg tggtacttca cgtcatccga gccgcccttc ggcgtgcggg
     2701 catcggtccc gccgcggccg gcctcgaact cggccaggat cgcctcgtac ggcacgccga
     2761 ccacgtgggc cagcacgttg aggcggccgc ggtgcgccat gccgatcacc acgtcccgcg
     2821 tgccggcggc ggcggcatcg gtcaggacgg tgtcgatcat gggcaccatg gcatccagcc
     2881 cctcgatgct gaaccgcttc tggcccaggt aggcccgatg caggaagcgc tcgaggccct
     2941 ccacctcggt caggcgcatg agcagcgcgc gtcgctcgtc agcgctcagc ggccgccggt
     3001 gcatgccgga ctcgatcgcc tcgcgcagcc acatgcgctc ctcgtgggag gccaggtgct
     3061 cgacctcgta ggcgatcgat ccgcagtagg tggctaccag gttggggagt gcctcggcca
     3121 gggtctcgcc ggggcaggcg atgcgcagca ggtcggcggg gatggcggcc atgacctccg
     3181 gggtgagccc cagcccggcc ggatccagcg cagggtcacc cggtggctgg ctgcccagcg
     3241 ggtccaattg ggctgccagg tggccgaagg accgataggc ccgcaccagg gcaaccgccg
     3301 cggcgatcgc cttgaggccg gccctgtccg gcgcgaactg tgcgctcgat gccggcggcg
     3361 gcgcgggttc cgcggcaggg gcggcgacat cggtccccag cgaggcaccc ttggcggtga
     3421 agacctcctc gtagaagccg tcctgcccgt cgagcagctc gtcgatgcgt cgcaggaagg
     3481 tcccggactc ggcaccctgg atgatgcggt gatcgtaggt cgacgagatg gtcatcaccc
     3541 gccgctcgcc cacgtcgcgg atggcgccgg tggccacgat cgtcccctgg cccggcatca
     3601 ggcgcgggac cgatgcggtg gtgcccagcg ttcccgggtt ggtgagggtg atggtggcgc
     3661 cggcaaagtc gtccggcgac agtctgttgg cccgcgaccg ctgtacgagc tcctcgtaac
     3721 gatcgaggaa ctcggcgaag tccatgccgt cggccccgcg gatgacgggc accaccagga
     3781 agcggctgcc gtcggggcgc tccacgtcga ccgccaggcc caggttcacc ccgcccgggt
     3841 cgacgcggtg cggcgcaccg tccacctggg tgaagtagtg ggtcatggac ggctggtcgc
     3901 tggcggcgcg cacgatggcc cagccgatga ggtgcgtaaa gctcaccttc gtgggcgcca
     3961 gctgaccatt cagctcgcga cgcctcgcct ccagcgtgcg gacctccacg tccctgaagc
     4021 tggtggcggt gggtacttcg agcgaggccg ccatgttgcg ggccaggctg gccgcgggcc
     4081 cccgaatcgg cgacgctccc ggcggcggtt cggcccgcgg tgcctcggcc tctggtgcct
     4141 cagccggggg ctgttcggcc gtcccggtgc ccccgtcggc cggtccgccg gagccgttgc
     4201 cgtcggacga agctgcggtt gccggcgcgg gtcgggcgac cggctcgaac ccgccggcgc
     4261 caccgtcgaa caggctccgc cattccggct ccaccgattc ggggtcgcgg cggtactgct
     4321 cgtagcgctc ggcgacgtag ccggcgttca cgctgtccaa cgcgatcgcc agcgaggaag
     4381 ggtcgtctcg ctccgtgggt ggcatctccc taaggatacg ggccggcggg ctgcgcttca
     4441 gcggacgcct tgctccagca cgcgggtcat gatcggacgg cccttccagt gctcggcgag
     4501 ccgctcgaac ccggcgcgct cgaagagatc cctggtaccg atgaagaccg atgcgctggc
     4561 cacgcgtcgc ccggcggtgt ccaccggata gccctccacg cggctggctc cggagcggcg
     4621 ggcatgatcg accgcggcat ccagcaggaa gcgcccgatc ccccggccac ggttcgtccg
     4681 cgggatgaag aagcagacca ccgaccatac cgatggatcc gaacgttcct cgggcgagag
     4741 acgcgtcacc ggcgagcgga ggatgcgacc gaactcaacg cgagggccga cgctgaccca
     4801 accgaccggc tggcggccgt cgaacgcgat caggcc
//
LOCUS       DATACA010000525         4946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1519046, whole genome shotgun sequence.
ACCESSION   DATACA010000525 DATACA010000000
VERSION     DATACA010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519046"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..1787
                     /locus_tag="VHK28_10610"
     CDS             423..1787
                     /locus_tag="VHK28_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX2142702.1"
                     /translation="MTALARPILRELMDRERRRFAEAHPRSAALFERSRVSLLGGVPM
                     NWMSKWPGSFPPFVERAGGGHFSCVDGHDYVDFCLGDTGAMAGHAPEASVQAIRAQLR
                     RGITTMLPTQDAPAVGEELARRFGLPFWQFTLTATDANRFAIRLARAITGRPRILVFN
                     YCYHGTVDETFIMLRDGVASPRDGNIGPAVDPAVTTRVVEWNDVPALEAALSHGDVAA
                     VLAEPVMTNIGIVHPEPGFHEALRNITRRTGTLLILDETHTICAGPGGYTAEHGLEPD
                     LLVVGKPIAGGLPAGAYGFTAQVAEQVAAHLELELTDVGGIGGTLAGNALSLAAMRAT
                     LSSVLTEDAYARTIPLARRWTDGVAGAIERSGLPWHVTRLGNRAEYLFGPNPPRNGGE
                     AAAMDDFELQQLTHLYALNRGILLTPFHNMALIAPQATEADVDHHTAVFEEMAGELSG
                     SGAG"
     gene            complement(1764..2234)
                     /locus_tag="VHK28_10615"
     CDS             complement(1764..2234)
                     /locus_tag="VHK28_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX2142703.1"
                     /translation="MTTGNDDWTRLPKAEFGFPRTDLRRRLVNAVLSGEKTATAGLLA
                     DFERDGDPLPRPGERYAVVDFDDRPVGVIETVEVRVVPMAEVDLQFAIDEGEGFGSVA
                     EWRTAHERFFSSYIDEIRAGVGDPGWELGDETMIVCERFRLVSPEPDSTRAAPA"
     gene            complement(2262..3899)
                     /locus_tag="VHK28_10620"
     CDS             complement(2262..3899)
                     /locus_tag="VHK28_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142704.1"
                     /translation="MRQAIATAIVSLHRVLASRDVRRAELAWVTGWTAEWAWQVALFV
                     HAFAVGGVVAVGALGLVRTLPAAVLAPMLSGLTDRLPRHRVLLTVHAGRATLIGLAVV
                     SILAGWPPAVIFVVAALDGLLAVLHRPTHMSLMPALARGPEELVAANLASSTLEAAGT
                     LVGPMVAGVLVAVANAPITFAVPAAAFAAAAAIVAGVEPTRQLARPSQTASGLRMLAG
                     GLGVLGRYPHAALLLGLFGLQTVTRGLLSVLIVVTAVDLLAIGEEGVGYLNAGLGAGG
                     LVGALLAMGLVGRGRMATPVYVGLVMWGVPILVMGVLPALGVALLAMAAVGAANAVLD
                     VAGFTLLQRSVPNAVRGSVFGTLEAIAMLGVGVGAALAPLLVTTLGPQAALVGSGLLL
                     PVAATLAWRWLRLTDAHAVIPEPELALLRGVPMFEPLPLTILEQLAEDLVAVRFEAGE
                     AIIREGDVGDRFYILRAGQATVEADGSELRRLDAGDSFGEIALLRDVPRTATVTAVTG
                     LDACALTRDAFLAAVTGDRRSMAAAEDTVRERLAASS"
     gene            3961..>4946
                     /locus_tag="VHK28_10625"
     CDS             3961..>4946
                     /locus_tag="VHK28_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX2142705.1"
                     /translation="MADHDPGPELAAERGEAQADAARRPVKVRDAVVRFGTASWTDPT
                     MTVPGVFYPPGTDSPEERLRYYAGQFPIVEVDATYYALPSRRMGEAWLERTPPDFTFD
                     IKAHALMTGQPSEVKRLPKEIRESLPMELLDRKRIYGKDLPSETYGAIWEWFVDALQP
                     LHSAGKLGAVFLQYPRWFFPSNESRDAIVEAHERLAGFGITAAVEFRNGTWFNEKNAE
                     RTVRFLEKYAIPFVMVDEPQTKTSLPPIVAVTSPDLAVVRFHGRRAETWEAKGIPVVE
                     RFRYLYDEQELGEWVPRVRQAAEGARNVHVLMNNCYANYGTTNARELAALIQS"
BASE COUNT          793 a         1807 c         1675 g          671 t
ORIGIN      
        1 gtagcccgcc tctgcacccg ggctgactgc cggcggtagc gccacggcgc tgaccggaac
       61 ccagctggga gtagcggccg agaccacggc cgggctggcc agggcggtca gggtgagaac
      121 catggaaagg cctagtgaga aggaacggat cgatagcctg cgagtcatcg cgtgagtgac
      181 ctctgtgctc ggtgcagccg acgctggggc acgggacgca acctgccgtc gagctgggtg
      241 ccgcaaggat aggcgaccgg cgcctgcgtg actagtactt ttccactatc ccccaccgga
      301 ccggaccgct ccggagagct ccagcgcggt gccccacaag agtgggccgg cggatgcgtt
      361 gactactgtc acctgcccga catcggttcg gtgagcttgg cgaccgtgcc aatatcggcc
      421 agatgacagc cctggcgcga ccgatcctgc gcgagctcat ggaccgcgag cgccggcgct
      481 tcgccgaggc gcaccctcga tcggccgccc tgttcgagcg atcgcgggtt tcactcctgg
      541 gcggcgtgcc catgaactgg atgagcaagt ggcccggctc gttcccgccg ttcgtggagc
      601 gtgccggcgg cggccacttc agctgcgtcg acggccacga ctacgtcgac ttctgcctgg
      661 gcgacaccgg cgccatggcc gggcatgcgc ccgaggcatc ggtccaggcc atccgggcgc
      721 agctgcggcg cggaatcacc accatgctgc ccacccagga tgcccccgcc gtcggcgagg
      781 agctcgccag gcgcttcggc ctgccctttt ggcagttcac cctgacggcg accgatgcca
      841 accggttcgc catccgcctg gcccgtgcca tcaccggccg gccacgcatc ctggtcttca
      901 actactgcta ccacggcacc gtcgacgaga ccttcatcat gctgcgcgac ggagtcgcat
      961 cgccgcgtga cggcaacatc ggtcccgcgg tggacccggc cgtgacgacc cgggtcgtgg
     1021 agtggaacga cgtgccggcc ctggaggcgg cgctgtcgca cggggacgtg gccgccgtgc
     1081 tggccgagcc ggtgatgacc aacatcggca tcgtgcatcc ggagcccggg tttcacgagg
     1141 cgctgcgcaa catcacgcgc cgcaccggca cgctcctgat actggacgag actcacacca
     1201 tctgtgccgg ccccggcggc tacaccgcag agcacgggct ggagccggac ctgctggtgg
     1261 tcggcaagcc gattgccggc ggcctgccgg ccggcgccta tggtttcacg gcgcaggtcg
     1321 ccgagcaggt ggccgctcac ctggagctgg agctgaccga tgtgggcggt atcggcggga
     1381 cgctggccgg caatgccctc tcgctggcag ccatgcgggc caccctctcg tccgtcctga
     1441 ccgaggatgc ctacgcgcgc accatcccgc ttgcccggcg gtggaccgat ggagtggcgg
     1501 gcgccatcga gcgcagcggc cttccctggc acgtcacccg gctcgggaac cgggcggagt
     1561 acctcttcgg cccgaacccg ccgcggaacg gcggcgaggc ggccgccatg gacgatttcg
     1621 agctgcagca gctcacccac ctgtacgcgc tgaaccgcgg gatcctgctc accccattcc
     1681 acaatatggc gctgatcgcg ccgcaggcca ccgaagccga cgtcgaccac cacaccgcgg
     1741 tcttcgagga gatggccggc gagctaagcg ggagcggcgc gggttgagtc gggttccggg
     1801 ctgacgaggc ggaaccgctc gcagacgatc atcgtctcgt cgccgagctc ccagcccggg
     1861 tcgcccacgc cggccctgat ctcgtcgatg taggagctga agaaccgctc gtgggccgta
     1921 cgccactcgg cgacgctgcc gaagccctcc ccttcgtcga tcgcgaactg gagatcgacc
     1981 tcggccatgg gcaccacccg cacctccacc gtttcgatca cgccgaccgg ccgatcgtcg
     2041 aagtcgacga ccgcgtaacg ctcgcccggc cgcggcagcg ggtcaccgtc gcgctcgaag
     2101 tccgccagca gcccggcggt cgcggtcttc tcaccgctca gcacggcatt gacgagacgg
     2161 cgtcgaaggt cggtgcgcgg aaagccgaac tcggccttcg gcaggcgcgt ccagtcgtcg
     2221 ttgccggtgg tcataggccg cgaagcatat ccgccgctgg gtcagctgct ggcagccagc
     2281 cgttcgcgaa cggtgtcctc ggccgccgcc atgctgcgcc ggtcgccggt gaccgccgcc
     2341 agaaaggcat ctcgcgtcag cgcgcacgcg tcgagcccgg tgacggctgt gaccgtcgcc
     2401 gtgcgcggga catcgcgcag cagggcgatc tcgccgaacg agtcgcccgc atcgaggcgc
     2461 cgcagctcgc tgccgtccgc ctcgaccgtc gcctggccag cccgcaggat gtagaagcgg
     2521 tcgcccacgt caccctctcg gatgatggcc tcgcccgcct cgaagcgaac cgccaccagg
     2581 tcctcggcca gctgctccag gatggtcagc ggcagcggct cgaacatcgg cacgccgcgc
     2641 agcagtgcca gctcgggctc ggggatcacc gcgtgggcat cggtcagtcg cagccagcgc
     2701 caggccagcg tggccgccac cggcagcagt agcccgctgc ccaccaatgc cgcctgcggc
     2761 ccgagggtgg tcaccagcag cggtgccagt gccgcgccga cgcccacgcc aagcatggcg
     2821 atcgcttcca gcgtcccgaa cacgctgccg cgcaccgcgt tgggcacact tcgctgcagg
     2881 agggtgaagc cggcgacgtc gagcacggcg ttcgcggcac ccaccgccgc catggccagc
     2941 agggctactc cgagtgccgg cagcacgccc atgacgagga tcggaacccc ccacatcacc
     3001 agcccgacat acaccggcgt cgccatccgg ccgcggccca ccagccccat ggccagcagc
     3061 gccccgacca gaccaccagc gcccaatccg gcgttcaggt acccgacgcc ctcctcgccg
     3121 atggccagca ggtcgacggc ggtgaccacg atcaggaccg agagcaggcc tcgcgtcacg
     3181 gtctgcaggc cgaacaggcc cagcaggagc gccgcatgcg ggtagcgtcc cagcaccccc
     3241 aggccaccgg ccagcatccg cagcccgctg gccgtttggc tcggccgggc cagctgcctg
     3301 gtgggctcca cgccggccac gatcgccgcc gcggctgcga aggcggcggc cggcacggcg
     3361 aaggtgattg gcgcgttggc gactgcgacc aggacgccgg ccaccatggg accgacgagc
     3421 gtgccggcag cctcgagcgt gctgctggcc aggttggccg ccaccagctc ctccgggccg
     3481 cgcgccaggg cgggcatcag cgacatgtgc gttggccgat ggagcacggc cagcaggcca
     3541 tccagtgcgg ctaccacgaa gatgacggcc gggggccagc cggcaaggat gctgaccacc
     3601 gccagcccga tcagcgtcgc tcgcccggcg tgcaccgtca gcaggacacg gtgcctgggc
     3661 aggcgatcgg tgaggccgga gagcatgggt gccaggacgg cggcgggcaa cgtgcgcacc
     3721 agccccaggg ccccgaccgc gaccacgccg cccaccgcga aggcatgcac gaagagggcg
     3781 acctgccagg cccactccgc cgtccagccg gtgacccacg ccagctccgc gcgccgaacg
     3841 tctcggctgg cgaggactcg gtgcagcgag acgatcgcgg tggcaatggc ctgcctcatg
     3901 agccgccgat gctagtcctg aggcgaggca tcggtccacc ttcggttcta gacttccggc
     3961 atggccgacc acgatccagg gccggagctg gcggccgagc gcggcgaggc gcaggccgat
     4021 gccgcccggc gacctgtgaa ggtgcgcgac gccgtcgtcc gcttcggcac cgcgtcctgg
     4081 acggatccga ccatgaccgt cccaggcgtc ttttacccgc cggggaccga tagccccgaa
     4141 gagcggctgc ggtactacgc cggtcagttc ccgatcgtcg aggtggacgc cacgtactac
     4201 gcactgccca gccgccgcat gggcgaggcg tggctggaac gcacgccgcc cgacttcacg
     4261 ttcgacatca aggcccacgc gctgatgacc ggccagccgt cggaggtcaa gcgcctcccg
     4321 aaggagatcc gcgaatcgtt gcccatggag ctgctggacc gcaagcgcat ctacggcaag
     4381 gacctgccca gcgaaaccta cggcgcaatc tgggagtggt tcgtggacgc cctgcaaccg
     4441 ctccactcgg ccgggaagct gggcgccgtc ttcctccagt atccgcgctg gttcttcccc
     4501 tccaacgaga gccgggatgc gatcgtcgag gcccatgaac ggctggccgg gttcgggatc
     4561 accgccgcgg tggagttccg caacggcaca tggttcaacg agaagaacgc ggaacgcacg
     4621 gtgcgcttcc tggagaagta cgcgatcccg ttcgtcatgg tcgacgagcc acagaccaag
     4681 acctcgctgc caccgatcgt ggcagtcacc tcgccggacc tggccgtggt ccggttccac
     4741 ggccggcgcg ccgagacctg ggaggccaag ggcattcccg tggtggagcg tttccgctac
     4801 ctgtatgacg agcaggagct cggcgagtgg gtgccgcggg tccgccaggc ggctgagggg
     4861 gcccgcaacg tccacgtgct catgaacaac tgctacgcca actacggcac caccaacgcc
     4921 cgtgagctgg cggcactgat ccagtc
//
LOCUS       DATACA010000526         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2242067, whole genome shotgun sequence.
ACCESSION   DATACA010000526 DATACA010000000
VERSION     DATACA010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242067"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1577)
                     /locus_tag="VHK28_10630"
                     /pseudo
     CDS             complement(1..1577)
                     /locus_tag="VHK28_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587539.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1878..>2028)
                     /locus_tag="VHK28_10635"
     CDS             complement(1878..>2028)
                     /locus_tag="VHK28_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX2142706.1"
                     /translation="NPVLFTAAIGLMLAWRVAGYYGLDRYLLPALGTPWRAPVSADAA
                     PPRGG"
BASE COUNT          292 a          722 c          728 g          286 t
ORIGIN      
        1 ccgtacaggc cacggtccgg gatggtgccg ccggaggtga tggccaccac ccgggcatcg
       61 ctgcgcgact ggacggtatc cgcctgccgg tcccacacca cgcgcggctt gagctatgcg
      121 aattcgtcgg agggatagcg cccggagagc atgtccagca cgccctccag ctgctcgcgc
      181 gacaggtcgc ggaacgggtg ggcgcgggtg accagggcgt gcaggtcagc caccgtccaa
      241 gcgtccagtg cgcacatggc caccacctgc tgggccagca cgtcgagcgg gttgcgcggg
      301 acgcgcgtct cctcgacggc accatccagc atgcgccgca caacgaccgc cgcctcgagc
      361 aggtcaccgc ggtacttggg aaaaatcttg ccggtggacg gctcacccac ctggtgcccc
      421 gcacggccga tgcgctgcag gccggacgcc accgatggcg gcgcctcgat ctggatgacg
      481 aggtcgatgg cgcccatgtc gatccccagc tcgagcgaac tggtggccac cagcgccggc
      541 aggcggccgg ccttgagcgc ctcctcgatg cccagccgct gctcgcgggc gatgctgccg
      601 tggtgcgcgc gcaccagctc ctccccggcc agctcgttca ggcgagcgga cagccgttcg
      661 gccaggcgcc gggagttgac gaagacgagc gtggaggtat gcgcccggat cagctccagc
      721 aggcgtggat agaccgatgg ccagatcgag tgcctggcct ccgggcctgc ggccgggcca
      781 ccgggctgct catcgagctc catctgctcg cccatgcggg ccatgtcgtc caccgggacg
      841 atgacctcca gctcgaggcg cttccgctca ccggcatcca cgacctccac ctcgcgctcc
      901 gggcctgagc cgcccaggaa ggcggcgatg gtgtccaacg gtcgctgggt ggccgacagt
      961 ccgatacgct gcggctggct tccggtcagg gcgcccagcc gctccaggct gagcgccagg
     1021 tgcgcgccgc gcttggtgcc ggcgatggcg tgcacctcgt ccacgattac gtgctccacg
     1081 ccgcgcagcg cctcgcgcgc ctggctggtg agcagcaggt acaggctctc gggggtggtg
     1141 accaggatgt ccggcgggtc cttggccagc tgccgtcgcg cctccgccgg cgtgtcgccg
     1201 gtccgcgagc cgacccgcac ctcaggcagg tggctcccct cccgctccgc ggtgcgccgg
     1261 atgccggcca gcggcgcccg aaggttgcgc tccacgtcgt aggtaagggc cttcagtggg
     1321 ctgacgtaca gcacccgcac gctgcgcgcg cggccctttg ccggcgccgg ctcagtgaac
     1381 aggcggtcca gcgtccagag gaaagccgcc agcgtcttgc ccgacccggt gggcgcgtgg
     1441 atcagcgtgt ggcggccgcg ggagatggcc tcccagccgc cggcctgggc cgtcgtgggc
     1501 gccgcgaacg cgtcgctgaa ccaggcgcgc gtgggcgccg agaacagggc gagcgggtcg
     1561 gcagtggcgc ggctcatgac gtgccccgca gtctacgtct caggcacgga tagaacaagc
     1621 gttctagcca gagcgagtgg gtggtgcggc tcgcgctatc tggcctgaac ggcccggcgc
     1681 gcagccatgg gatggcccca aaggccaagc agcgcccgaa tcgaggctgt tcttggccgc
     1741 ggtggtcacc ggacggcgcc agcagccatt aaggttcgat ttggctctct tcttggtcgt
     1801 tagagccatt cggtgagcac gagcacccac tcgcggctat ccaccggggc cggcacgtcg
     1861 ggcagccacg ggtggcttca acctccgcga ggcggagccg cgtccgcgct gaccggcgcg
     1921 cgccacggcg tgcccagcgc aggcagcagg tagcggtcca gcccgtagta gccggccacg
     1981 cgccaggcga gcatcaggcc gatggcggcg gtgaacagca ccgggttg
//
LOCUS       DATACA010000527         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2161472, whole genome shotgun sequence.
ACCESSION   DATACA010000527 DATACA010000000
VERSION     DATACA010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161472"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..615)
                     /gene="rpiB"
                     /locus_tag="VHK28_10640"
     CDS             complement(166..615)
                     /gene="rpiB"
                     /locus_tag="VHK28_10640"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX2142707.1"
                     /translation="MRVAIGSDHAGFGLKGELGTLLDELRIEYRDFGTFSEEPVDYPD
                     FIAPVARAVARGDYRFGIVLGGSGTGEAIVANKVRGIRCVQATNPVVARLAREHNDAN
                     CIAFGARIIGTEVAKASLREFLTAEFQGGRHVPRIEKIARLEAGEAG"
     gene            complement(758..1366)
                     /locus_tag="VHK28_10645"
     CDS             complement(758..1366)
                     /locus_tag="VHK28_10645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX2142708.1"
                     /translation="MTERLPPTAVGIAAAAAILREGGLVAFPTDTVYGVGVAASRRDR
                     LDALFALKHRPLDRRIPMLVADLNSLPPGWMVDDRARVLADRFWPGALTLVLPAEEAG
                     GDSQAFRAPNHPVAQDLIREAGPLLATSANISDQPDTLDADEVLVAFATQNVELDAVL
                     DGGPVPGGVASTVVDLTVEPAAILREGPIEATTLADVVALSD"
     gene            complement(1363..2271)
                     /gene="prmC"
                     /locus_tag="VHK28_10650"
     CDS             complement(1363..2271)
                     /gene="prmC"
                     /locus_tag="VHK28_10650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX2142709.1"
                     /translation="METQVVNPDARSLVAAATQRLRASGSDTARLDAEVLVAHAFGRD
                     RTWLAAHPEAELGSTERAALEAWVARRADGEPVAYIRGFKEWLSLRIRTDRRALIPRP
                     ETELLTEAAIAEVAARLAGDDRPIAIWEVATGSGAVAVALALRFRTALALGRVRLAAS
                     DASAEALELAAENLSAHGVAGLVTLGAGDLLEPAVLAAPLQPDVLVANLPYLTSEEAA
                     AGHGSLAWEPRVALDGGADGLDLVRRLLAELPGRLAPGGVALLEIGAGQADTVRELAA
                     ALPMPAAIQTVPDLAGIERVVRVRRT"
     gene            complement(2282..>3054)
                     /gene="prfA"
                     /locus_tag="VHK28_10655"
     CDS             complement(2282..>3054)
                     /gene="prfA"
                     /locus_tag="VHK28_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX2142710.1"
                     /translation="PSDDRDVILEVRAGTGGDEAALFAADLFRMYARYAERNRWKLEL
                     LSSSEGAAGGFKEVIAEVRGPGAYSRLKFESGVHRVQRVPVTEAQGRIHTSTATVSVL
                     PEAEEVEIDIPEGDLRVDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVAIQDEKSQHK
                     NKAKAMAVLRARLLEMEQQRQAEERGEQRRSQVGSGERSEKIRTYNFPDDRVTDHRIG
                     LTVHNLPGLLEGDLDRVVDPLADADQAARLAEVGMDGA"
BASE COUNT          422 a         1142 c         1056 g          434 t
ORIGIN      
        1 ggcaacaagc accagccaga cactccggcc tggccgggag ttgcgccgga ggacaacgcg
       61 ggccacgtgc acgggagggg catcgtcatg gtttgcacgg gggtggcaag ccgcgacagg
      121 acgggccggg taccttgcgg ttgttgccgt ccggtgcaaa cgcggctacc ccgcctcccc
      181 ggcctccagg cgggcgatct tctcgatgcg cggcacgtgc cggccgccct ggaactcggc
      241 tgtcaggaac tcccgcaggc tggccttcgc cacctcggtg ccaatgatcc gcgcgccgaa
      301 cgcgatgcag ttggcgtcgt tgtgctcgcg ggccagccgc gccacgaccg ggttggtggc
      361 ctgcacgcat cggatgcccc tgaccttgtt ggccacgatc gcctcgccgg tcccgctgcc
      421 gcccagcacg atgccaaatc ggtagtcgcc gcgcgccact gcccgggcca ccggagcgat
      481 gaagtccgga tagtccaccg gctcctccga gaaggtgccg aagtcgcggt actcgatgcg
      541 cagttcgtcc agcagggtcc ccaactcacc cttgagcccg aatccggcgt gatctgaacc
      601 gatggcgacg cgcatgcggg cagtctacgc agccgtccag ccgccgggcg gagtgccggt
      661 gcgctgtgga gggccggttg tgcggctgtg ctcctgtgcg gctgtgctcc tgtgcggctg
      721 tgcggcacag acgacccctg acagccgccg cgggggctca gtcggacagt gccaccacgt
      781 cggccagcgt ggtcgcctcg atcggcccct cgcggaggat cgcggccggt tccacggtca
      841 ggtccaccac agtggaggcc acccctcccg gcacgggccc gccgtccagc accgcgtcca
      901 gctccacgtt ctgggtggcg aaggcgacca gcacctcgtc ggcatcgagc gtgtccggtt
      961 ggtccgagat attggcgctg gtggccagca gcggccctgc ctcgcggatg aggtcctggg
     1021 cgaccgggtg gttcggcgcc cggaaggcct gcgagtcgcc gcccgcctcc tccgccggca
     1081 gcaccagggt cagggcgccc ggccaaaagc ggtcggccag cacgcgcgcc cggtcgtcca
     1141 ccatccagcc gggcgggagg gagttgaggt cggccaccag catcgggata cgtcgatcga
     1201 gcggacgatg cttgagtgcg aacagggcgt ccagccgatc gcggcgcgag gcggccacgc
     1261 ccacgccata gacggtatcg gtcgggaagg cgaccagccc gccctcgcgc aggatggcgg
     1321 ccgctgccgc gatgcccacc gccgtaggcg gcagccgctc ggtcacgtgc gccggacccg
     1381 gaccactcgc tcgatgccag ccaggtccgg caccgtctgg atggctgcgg gcatcggcaa
     1441 tgcggcggca agctcgcgca ccgtgtccgc ctgcccggca ccgatctcca gcagcgcgac
     1501 cccgccgggc gccaaacgtc cgggcagctc cgccaggagg cgacgcacga ggtcaagccc
     1561 gtcggcaccg ccgtccaagg cgacccgcgg ttcccaggcc agcgagccat gtccggctgc
     1621 cgcctcttcc gaggtcaggt agggaaggtt ggccaccagc acgtccggct gcaacggagc
     1681 cgccaggacc gccggctcca gcaggtcccc ggcgcccagg gtcaccagtc ccgccacgcc
     1741 gtgggccgac aggttctcgg ccgccagctc gagcgcctcg gcggacgcgt ccgacgccgc
     1801 cagccgcacg cggcccagcg ccagcgcggt gcggaagcgg agcgccaggg caaccgccac
     1861 cgcgccactc cccgtggcca cttcccagat ggcgatcggc cggtcgtcgc cggccaggcg
     1921 agccgcgact tcggcaatgg ccgcctcggt aagcagctcc gtctcgggac gcgggatcag
     1981 cgcccgccgg tcggtccgga tgcggagcga gagccactcc ttgaagccac ggatgtaggc
     2041 caccggctcc ccgtcggccc gccgcgccac ccatgcctcc agcgccgcgc gctccgtgga
     2101 tcccagctcc gcctcggggt gcgccgccag ccaggtgcga tcccggccga aggcatgggc
     2161 caccagcacc tcggcatcga gccgggcggt atccgagccg gacgccctca gccgctgcgt
     2221 cgccgcggcg accagggatc gggcgtcggg attgacgacc tgcgtctcca tcggtccact
     2281 gtcaggcgcc atccatgccc acctcggcca ggcgtgcggc ctggtccgcg tccgccagcg
     2341 ggtcgaccac ccggtccagg tcgccctcca gcaggccggg aaggttgtgg acggtcaggc
     2401 cgatgcgatg atcggtgacc cggtcatccg ggaagttgta ggtccggatc ttctccgagc
     2461 gttcgccgct gcccacctgg gagcgacgct gctcgccacg ttcctcggcc tgccgctgct
     2521 gctccatctc cagcagccgc gccctcagca cggccatggc cttggccttg ttcttgtgct
     2581 ggctcttctc gtcctggatg gcgaccacca ggccggtcgg cttgtgcgtg atgcggacgg
     2641 ccgagtcggt cgtgttcacg ctctgcccgc cgggaccact ggaccgatat acgtcaaccc
     2701 gcaggtcccc ctccgggatg tcgatctcca cctcctccgc ctcggggagg acgctgaccg
     2761 tcgccgtgga ggtgtggatg cggccctgcg cctcggtgac cggcacgcgc tggacgcggt
     2821 gcacgcccga ctcgaatttc agcctcgagt aggcgccggg cccgcgcacc tcggcgatga
     2881 cttccttgaa gccgcctgcc gcgccctcgg acgaagagag gagctccagc ttccagcggt
     2941 tgcgctccgc gtagcgggcg tacatgcgga acaggtcggc cgcgaacagg gctgcctcgt
     3001 cgccgccggt tccggcacgg acctccagga tcacgtcacg gtcgtcgctc gggt
//
LOCUS       DATACA010000528         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2482721, whole genome shotgun sequence.
ACCESSION   DATACA010000528 DATACA010000000
VERSION     DATACA010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482721"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VHK28_10660"
     CDS             complement(<1..196)
                     /locus_tag="VHK28_10660"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX2142711.1"
                     /translation="MRRRYREAGVDLDEARRAVELIGRAAASTATPSVIGGVGGFGSL
                     FRFDPAPYPDPVLVASTDGVG"
     gene            complement(206..1678)
                     /gene="purF"
                     /locus_tag="VHK28_10665"
     CDS             complement(206..1678)
                     /gene="purF"
                     /locus_tag="VHK28_10665"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2142712.1"
                     /translation="MLPVYQSHGPREECGVVGIYAPGAPVARLAYVGLHALQHRGQES
                     AGIATSDGARLVLHKRLGLVSQVFDEETLARLDRGDAELGANGARIAIGHTRYSTTGS
                     NSLPNVQPVYAGSDLGELMLGHNGNLTNATWLRDELAEQGVEFGTASDTEVLARLIAH
                     APGRTWAQRVEHAFHRAVGAYTFTMLTEGALVAARDPIGFRPLALGRITDADGTPLGW
                     AVASESAALDAMGATFVRDVEPGEVLTVDAAGVHSHRPPAPAAAERLCVFEFVYLARP
                     DSVIGGRLLYEARLEMGRRLALEHPVEADVVIPVPDSAIPAAIGYAEQAGLPFREGLI
                     KNRYVGRTFIQPVQRSREASVQMKFNPLPEVLANKRVVVVDDSIVRGTTTGPIVSMLR
                     RAGAREVHVRIHSPQMRFPCYMGVDTGRRSELIAANQGLEEIRRTIGADSLGYLSQEG
                     LLAAIGRERDGERRHCTACFDGRYPVDVPLDVDKFALERG"
     gene            1931..3622
                     /locus_tag="VHK28_10670"
     CDS             1931..3622
                     /locus_tag="VHK28_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX2142713.1"
                     /translation="MLRLARALGFLAPLAVALFVTVGFAVTTRAGDDQQRTTQLILAG
                     LAAAAGMTSMALFAWSYVGWRLNRIARSLEDTLEADSPVLLRETGIPAERRLARAFNA
                     ATGAFLQVEARATHDRLTGVANRETLLTTLVAEVERAVRHHKWLSVAFIDIDRFKPIN
                     DTYGHNSGDAVLRQVAGLVADNIRGSDLFGRYGGEEFMLILPETSPDEAVVLAEKLRT
                     QLMQQPLVIAGNQRLQVTISIGVAGDVGSQLQVDALVDQADAAMYAAKSLGRNRTYLF
                     KAIDENAPVRRAPISAEHRAAATAIGRWASDTATEALASVLAPQPHHRGRPSDMIASL
                     ATGIALELGLPHEEIERVRIASLLHDLGKLAVPSDILDKPTALSDGEWQAIGEHPRIG
                     QVILEGASSLREAIPVVLHHHERFNGGGYPHGLRGNEIPLGARIVAVADAYHAMIHDR
                     PYKKALSHEAALDELRANAGTQFDPDVVEVFCSIYADGVPPDGLEEVYRLHERARGGL
                     EEIDPHAAAAVLAGTELDRAHGHSHAPPAAPRGRRKKREPAADEPRPRPIREAAG"
     gene            3619..>4643
                     /locus_tag="VHK28_10675"
     CDS             3619..>4643
                     /locus_tag="VHK28_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142714.1"
                     /translation="MTRWRLQIIVLAAVAALMVAPSSASAAPPNQLHAPAVTPLDGGP
                     STIFTFSVRYVSTGGNPAFGVTATAGGRTVALTLVSGNATGGTWSGTSTLPPGTWTVR
                     FRATADRGPSPQATGPTVSVTTPPTPEPPGGGGGGGKTTSLPPEPSAPAVAPTQPGGN
                     AAAPSLDAAPVPGSTVPSPSQGAGTTADPPVGDPPGTTGGVTGTGGGGGQSVSGGAGT
                     PAPGSTGAAPASSGGSAPAATAANAPGATAAAGSVSGPSPRDMADRALVWTVLAIGLV
                     GVSAVGLLGVVWVLRGRDEDEDEPVPASVTPAPTKPDDAIAPLLQQRMMRRARMRSSE
                     DPILAAMG"
BASE COUNT          648 a         1739 c         1633 g          623 t
ORIGIN      
        1 tgcccacccc atcggtcgag gcaaccagca ccgggtcggg gtagggcgcc gggtcgaagc
       61 ggaagaggct gccgaagccg cccaccccgc cgatcacgga cggcgtggcg gtcgacgccg
      121 ccgcacggcc gatgagctcg acggcgcgcc gcgcctcgtc caggtccact ccggcctcgc
      181 ggtaacgccg tcgcatctgg ccctcctacc cgcgttccag cgcgaacttg tccacgtcga
      241 gcggcacgtc cacgggatag cgaccatcga agcaggccgt gcagtgccgg cgctccccgt
      301 cccgctcgcg gccgatggcg gccagcagcc cttcctgcga caggtagccg agcgagtcgg
      361 cgccgatggt gcgccggatc tcctccagcc cctggttggc ggcgatcagc tccgaccgtc
      421 gcccggtgtc cacgcccatg tagcacggga accgcatctg cggggagtgg atgcgcacgt
      481 gcacctcgcg ggctcccgcg cggcgcagca tggacacgat cggtccggtg gtcgtgccgc
      541 gcacgatcga atcgtccacc accaccaccc gcttgttggc cagcacctcg ggcagcgggt
      601 tgaatttcat ctggaccgat gcctcacgcg accgctgcac gggctggatg aaggtgcggc
      661 ccacgtatcg gttcttaatc agtccctcgc ggaagggcag gccggcctgc tctgcgtagc
      721 cgatggccgc cgggatggca ctgtccggca ccgggatgac cacgtccgcc tccaccgggt
      781 gctccagcgc caggcggcgg cccatctcca ggcgcgcctc gtacagcagg cggccgccaa
      841 tgaccgaatc cgggcgcgcc aggtacacga actcgaagac gcacagccgc tcggcggcgg
      901 ccggggccgg cggtcgatgc gaatgaacgc ctgcggcgtc cacggtcagt acctcgccgg
      961 gctccacgtc gcgcacgaag gtggcgccca tggcgtccag cgccgcgctc tcggaggcca
     1021 ccgcccagcc gagcggcgtc ccatccgcgt cggtgatgcg gcccagcgcc agcggccgga
     1081 agccgatggg gtcccgcgcg gcgacaaggg cgccctcggt gagcatggtg aaggtgtagg
     1141 cgccaacggc ccgatggaag gcatgctcca cccgctgcgc ccaggtgcgg ccgggggcgt
     1201 gcgcgatcag gcgggcgagc acctcggtgt ccgaggcggt gccgaactcc acgccctgct
     1261 cggccagctc gtcgcgcagc caggtggcgt tggtcaggtt cccgttgtgg ccgagcatca
     1321 gctcgcccag gtcgctgccg gcgtacaccg gctgcacgtt ggggagcgag ttactgccgg
     1381 tggtcgagta gcgcgtgtgg ccgatggcga tacgcgcgcc gttggctccc agctccgcgt
     1441 cgccgcggtc caggcgggcc agggtctcct cgtcgaagac ctggctgacc agccccagac
     1501 gcttgtggag gacgagccgc gctccgtcgc tggtggcgat gccggccgat tcctggccgc
     1561 ggtgctggag ggcatgcagc cccacgtagg cgagccgcgc gaccggcgcg ccgggggcat
     1621 agatgccgac caccccgcac tcctcgcgcg gtccgtgcga ctggtagacg gggagcatcc
     1681 ggtcagctgg ggcggcgggg aggcacgcgc cgatgatagc ccttcggccc gccgcgccgg
     1741 gggcgctcga cgcgcccccg cagtgccgaa cgagggtacc aatggggtac tgcaccgcac
     1801 cgcacagcgg ccgaatcgag gcggcgcgcc ggtgtgccac ctcgcaacgc ctgaccatcg
     1861 ggccatggcc gacggaccct cggcaggcca tcctgcacct cgctagtcac accaccctcg
     1921 tggaggttgc atgctccgtc tggcccgcgc gctgggtttc cttgctccgc tggcggttgc
     1981 cctgttcgtg acggtcggtt tcgccgtcac caccagggcc ggcgacgatc agcagcgaac
     2041 gacccagctg atcctggccg gactggcggc ggcggccggc atgacctcca tggcgctctt
     2101 tgcctggagc tacgtcggct ggcggctgaa ccggattgcg cgctcgctgg aggacaccct
     2161 cgaggcggac agcccggtcc tccttcgcga gaccggcatc ccggccgagc gccgccttgc
     2221 gcgcgccttc aatgccgcca ccggggcctt cctccaggtc gaggcgcgcg ccacccacga
     2281 ccgcctgacc ggggtggcca accgcgagac gctgctcacc accctggtcg ccgaggtcga
     2341 gcgcgcggtg cgccaccaca agtggctgtc ggtggccttc atcgacatcg accgcttcaa
     2401 gcccatcaac gacacctacg gtcacaactc gggtgacgcc gtgctgcgcc aggtggccgg
     2461 gctggtggcc gacaacatcc gtggctcgga cctcttcggg cgttacggcg gggaggagtt
     2521 catgctcatc ctccccgaga cgtcgccgga cgaggcggtg gtgctggccg agaagctgcg
     2581 cacccagctc atgcagcaac cgctggtgat tgccggcaac cagcggctac aggtcaccat
     2641 cagcatcggc gtggcgggtg acgtgggctc gcagctccag gtcgacgcgc tggtcgacca
     2701 ggccgatgcg gccatgtacg ccgccaagtc gctgggacgc aaccggacct acctgttcaa
     2761 ggccatcgac gagaacgccc ccgtccgccg cgcgcccatc tcggccgagc accgcgccgc
     2821 cgccaccgcc atcggtcgct gggcgagcga caccgccacc gaggcgctgg catcggtcct
     2881 ggcaccgcag ccccaccacc gcgggcggcc gtcggacatg atcgcttcgc tggcgaccgg
     2941 catcgccctg gagctggggc tgccgcacga ggagatcgag cgggtgcgga tcgccagcct
     3001 gctccacgat ctcggcaagc tggcggttcc ctccgacatc ctcgacaagc cgaccgccct
     3061 gtcggacggc gagtggcagg ccatcggcga gcacccccgc atcggccagg tcatcctgga
     3121 gggtgcctcc agcctgcggg aggcgatccc ggtcgtcctc caccaccacg agcggttcaa
     3181 cggcggcggc tacccgcacg ggctgcgcgg caacgagatc ccgctgggcg ctcgcatcgt
     3241 ggccgtggcc gatgcctacc acgccatgat ccacgaccgc ccgtacaaga aggcgctcag
     3301 ccacgaggcc gcgctcgatg agctgcgcgc caacgctggt acccagttcg acccggacgt
     3361 ggtcgaagtc ttctgctcca tctacgccga tggcgtgccg cccgacgggc tcgaggaggt
     3421 gtaccgcctg cacgagcggg cgcgcggcgg gctggaggag atcgatcccc acgccgcggc
     3481 agccgtcctg gccggcacgg agctcgaccg tgcgcacggt cacagtcacg cgcctcccgc
     3541 ggcgccgcgc ggacgacgca agaagcgaga gccggccgcg gacgagcctc gcccgcgccc
     3601 cattcgggag gccgccgggt gacgcgctgg cgcctccaga tcatcgtcct tgcagcagtg
     3661 gcggcgctca tggtggcgcc gtcgtcggca tctgcggcgc ctccgaacca gctccacgcg
     3721 ccggccgtca cgccgctgga cggaggcccg tccaccatat tcacgttcag cgtgcgctac
     3781 gtctcgacgg gcgggaaccc ggcattcggc gtcaccgcca ctgccggcgg gcggacggtg
     3841 gcgctcaccc ttgtctccgg caacgcgacg ggcggcacct ggagcggtac ctcgacgctt
     3901 ccgcccggga cgtggaccgt ccgcttcagg gccacggcgg accgcggccc gtcgccgcag
     3961 gccaccgggc caaccgtcag cgtaaccacc ccacccaccc ccgagccgcc gggtggcggt
     4021 ggcggcggtg gcaagaccac ctccctgccg ccggagccct ccgcgccggc ggtggctcca
     4081 acgcagcccg gcggaaacgc ggccgctccc agcctggatg cggcgcccgt cccgggctcg
     4141 actgtaccct cccccagcca gggtgccggc acgaccgccg atccgccggt gggtgaccca
     4201 cccggcacca ccggtggtgt gaccggcacg ggcggaggcg gtggccagtc cgtctcgggt
     4261 ggtgccggca cgccggctcc cgggtcgacc ggggcggcgc cggcctccag tggaggctct
     4321 gctccggcgg ccacggccgc caacgcaccg ggggccacgg ccgcggcagg gtcggtcagc
     4381 gggccatcgc cgcgtgacat ggcggatcgc gcgctggtgt ggacggtcct ggccatcggc
     4441 ctggtcggcg tgtccgcggt cgggctgctc ggggtggtgt gggtgctccg tggacgggat
     4501 gaggatgagg acgaaccggt cccggcgtcc gtcaccccgg cgccgaccaa gccggacgat
     4561 gccatcgctc cgctccttca gcagcgcatg atgcgccgcg cccggatgcg atccagcgag
     4621 gaccccatcc tggccgccat ggg
//
LOCUS       DATACA010000529         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2082161, whole genome shotgun sequence.
ACCESSION   DATACA010000529 DATACA010000000
VERSION     DATACA010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082161"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHK28_10680"
     CDS             complement(<1..593)
                     /locus_tag="VHK28_10680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2142715.1"
                     /translation="MNELPPEIAAILDERSEARQARDWKRADALRDRLHQLGWEPVDS
                     PSGSTARPRPSGAAAGVGYLGDQAGSLLDEPPTLGASVVVVADEQEADVTRFLDGMRR
                     HPPAIDWEMVLVANAPSFEVAAGDELPATAVSTSARVGWADAANLGMRRTRGAVIVLA
                     DTSLEPTGDWLTPLHAAFEDPTVGIAADHAPWTGDAHR"
     gene            complement(600..983)
                     /locus_tag="VHK28_10685"
     CDS             complement(600..983)
                     /locus_tag="VHK28_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX2142716.1"
                     /translation="MPVFDRHVFVCTRGEWCPSVDGDGIGVHAALKQAVRDAGLSATV
                     RVNHAGCFSQCGNGPMAVVYPDGVWYAALTPGDAEEIVREHLVGGRPVERLRYDPAEP
                     GAHKLERAPDGRPIGRSGAWPAGEG"
     gene            1135..1317
                     /locus_tag="VHK28_10690"
     CDS             1135..1317
                     /locus_tag="VHK28_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2142717.1"
                     /translation="MSNEVGKLLTASEVAEMLHLHVNTVKRLGDRGELPFFRVTRRGD
                     RRFRYDDVVEFLRREK"
     gene            1442..2593
                     /locus_tag="VHK28_10695"
     CDS             1442..2593
                     /locus_tag="VHK28_10695"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2142718.1"
                     /translation="MTDDARRRLAEGQRLLDENDPEGAIGLLEPLTSNRAVAEEAWLA
                     LATARYRTDDEAGALDAWQAAADLDGPTAWLGWRSAAEQHVRNGDLDEAIGAYREADR
                     RAPPHERGAIANRIAWLLKETGHDFAARRQFNRARGAYATYPAYVTWAIVIVNLAIFG
                     LDALLTGFGSLQLWGAGGPLVEAGLVSAPAVAAGEWWRLFTSAFLHLGILHIAFNMYA
                     LWLFGPIIEQLYGHVEYVVLYVLCALGGSVLTIVASPDSAAAGASGAIFGLFGLAFVV
                     SRRRHLLLGPQARAILSQAGGLLLINLVITFTVPRISWTGHLGGLAIGVAIGLLLSPR
                     NVPTMGGLWRAPDGAPLAGGTSLAVRAAAYLGVAAVLLAGTWFAVQQVG"
     gene            2603..3358
                     /locus_tag="VHK28_10700"
     CDS             2603..3358
                     /locus_tag="VHK28_10700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX2142719.1"
                     /translation="MCYDADARPPLPPIRGSALDAGEQVLTSADGTQVAAYAARAEGD
                     DRPGIVVLPDVRGLHPFYEELALRLAEAGMHAIAIDYYSRTAGTGRRGEGFDYQPHVE
                     QMGHETLNQDVAAAVGFLRSSAGGEANRVYTVGFCVGGRISYLQAAAGHRLSGVIGFY
                     GSLAKPHRSGLPSPVDVADQFECPVLALWGGADQGIGPEAVEAFTTALREAGVEHRSV
                     VYPGAPHSFFDRHQPDYAEASEDAWRQLLDFVS"
     gene            complement(3368..>3554)
                     /locus_tag="VHK28_10705"
     CDS             complement(3368..>3554)
                     /locus_tag="VHK28_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142720.1"
                     /translation="TGPILIVGQLLRALFGYSSTPEWATLIVWLAYLAVVLPLYLRPV
                     KPVAPKPVTGGQPEAAA"
BASE COUNT          484 a         1287 c         1264 g          519 t
ORIGIN      
        1 cggtgggcat cgccggtcca tggggcgtgg tcagcggcga tgcccaccgt cgggtcctcg
       61 aacgcggcat gcagcggtgt cagccagtcg ccggtcggtt cgagcgaggt atcggccagg
      121 acgatcaccg cgccccgcgt gcgccgcatg ccgaggttgg cggcgtcggc ccagcccacc
      181 cgcgcggagg tcgagacggc ggttgccggt agctcatcgc ccgccgccac ctcgaacgac
      241 ggcgcgttgg ccaccaggac catctcccag tcgatggccg gcggatggcg gcgcatgccg
      301 tccaggaagc gcgtcacgtc cgcctcctgc tcgtcggcca ccacgaccac cgaggcgccc
      361 agggtgggag gctcgtcgag gagtgagccg gcctggtcgc cgagataccc gacgccggcg
      421 gctgcgccgc tgggtcgcgg ccgcgcggtc gagccggagg gggagtccac cggctcccag
      481 cccagctggt gcagccggtc gcgcagcgcg tcggcgcgct tccagtcgcg ggcctggcgc
      541 gcctcggacc gctcgtccag gatggcggcg atctccggcg gcagctcgtt catccggcct
      601 cagccctccc cggccggcca ggctccgctg cggccgatgg ggcggccatc gggcgcgcgc
      661 tcgagcttgt gggcccccgg ctcggccggg tcgtagcgca gccgctccac cggtcgcccg
      721 ccgaccaggt gctcgcggac gatctcctcc gcgtctcccg gggtcagcgc cgcgtaccag
      781 acgccatcgg ggtagacgac ggccatcggt ccattgccgc actggctgaa gcagcccgca
      841 tggttcaccc gaacggtggc tgacaggccg gcatcgcgca cggcctgctt cagcgcggcg
      901 tgtacgccga tcccgtcccc gtccaccgac gggcaccact caccgcgtgt gcagacgaaa
      961 acgtggcggt cgaagactgg catctcgaaa ggtacccctg aggccgagcc gaaagtaccg
     1021 ggtgtccgga gggcacccca accttactat ccggccaaga gccgcgccct ccgcacccaa
     1081 gcagtgcggg cgcagccgga acacaccgct ctggtagagg catcaccacc aatgatgagc
     1141 aacgaggtcg ggaagctgct cacggcgtcc gaggtcgccg agatgctgca cctgcacgtc
     1201 aacaccgtca agcgcctcgg cgaccgcggc gagctgccct tcttccgggt cactcgccgt
     1261 ggggaccgac gcttccggta cgacgacgtg gtcgagttcc tccgccggga gaagtagacc
     1321 acccaatcga tgacgtcccg gtttcgccgg gacggacggt tcccgtcagg ggggctgcgg
     1381 acgaccgccg aggtgccaaa ttctcggcgg tcgctcgctg tctgggataa tgcgccccca
     1441 catgaccgat gacgcccgcc ggcgcctggc cgaaggccag cgcctcctgg acgagaacga
     1501 tcccgaaggg gccatcggcc tgctcgagcc gctgaccagc aaccgcgcgg tggccgagga
     1561 ggcctggctc gcgcttgcga ctgcccgcta tcggaccgat gacgaagccg gtgcgctcga
     1621 tgcctggcag gccgccgccg acctggacgg acccacggcc tggctcggct ggcgctcggc
     1681 cgccgagcag cacgtgcgca acggtgatct tgacgaggcc atcggcgcct accgcgaggc
     1741 ggaccgccgg gcgccgccgc acgagcgcgg ggcgatcgcc aaccgcatcg cctggctact
     1801 gaaggagacc ggccacgact tcgccgcgcg gcgtcaattc aaccgcgcgc ggggcgcgta
     1861 cgccacctat ccggcctacg tcacctgggc gatcgtgatt gtcaacttgg ccatcttcgg
     1921 gctcgacgcg ctgctgaccg gcttcggcag cctgcagctg tggggcgctg gtggcccgct
     1981 ggtcgaagcc ggattggtct cggcgccggc ggtggcggcc ggggagtggt ggcgcctgtt
     2041 caccagcgcc ttcctgcacc tcgggatcct gcacatcgcc ttcaacatgt acgcgctgtg
     2101 gctgttcggc cccatcatcg agcagctgta cggccacgtg gagtacgtcg tgctctacgt
     2161 gctgtgcgcg cttggcggca gcgtgctgac catcgtggcc agcccggact cggccgcggc
     2221 cggagcatcg ggcgccatct tcgggctctt cggcctggcc ttcgtcgtct cgcgccggcg
     2281 tcacctgctg ctcgggccgc aggcgcgagc catcctgtcg caggccggtg ggctgctgct
     2341 catcaacctg gtcatcacct tcaccgtgcc gcgcatcagc tggaccggcc acctgggcgg
     2401 cctggccatt ggcgtggcca tcggcctgct gctgtcgccg cgcaacgtgc cgaccatggg
     2461 cggcctgtgg cgagcgccgg acggcgctcc gctggccgga ggtacctcgc tcgcggtacg
     2521 ggccgccgcc tacctgggcg tggcggccgt gctgctggcg ggcacctggt tcgcggtcca
     2581 gcaggtcgga taggaggagt ccatgtgcta cgacgctgat gctcgacccc cgctgccacc
     2641 catccgcggc tcagccctgg acgccggtga gcaggtcctc acctcggctg acgggacgca
     2701 ggtggcggcc tacgcggcgc gcgccgaggg cgatgaccgc ccggggatcg tggtcctgcc
     2761 agacgtgcgc ggcctgcacc cgttctatga ggagctggcc ttgcggctgg ccgaggccgg
     2821 catgcacgcc atcgccatcg actactacag ccgcacggcc ggtaccggcc gccgcggtga
     2881 ggggttcgac taccagccgc acgtggagca gatgggccac gagaccctca accaggacgt
     2941 cgcggcggcg gtcggcttcc tccgctcgtc cgccggtggc gaagcaaacc gcgtctacac
     3001 ggtcggcttc tgcgtgggcg gtcgcatcag ctacctgcag gccgcggccg gacaccggct
     3061 gtcgggggtc atcggcttct acggctcgct ggccaagccg catcggtccg gcctgccgtc
     3121 accggtggac gtggcggacc agttcgagtg tccggtgctg gcgctgtggg gaggcgccga
     3181 ccagggcatc ggtcccgagg ccgtggaggc cttcacgacg gcgctgcgcg aagcgggcgt
     3241 ggagcaccgg agcgtggtgt accccggtgc gccgcactcc ttcttcgacc ggcaccagcc
     3301 ggactacgcc gaggcgtccg aggacgcctg gcgacagctg ctcgacttcg tctcctagct
     3361 gggccgccta ggcggcggcc tcgggctgcc cgccggttac cggcttgggg gccaccggct
     3421 tgaccgggcg caggtagagc ggcagcacca ccgccaggta ggccagccac acgatcaagg
     3481 tcgcccactc cggcgtgctc gagtagccga acagggcgcg cagcagctgg ccgacgatca
     3541 ggatgggtcc ggtc
//
LOCUS       DATACA010000530         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_395168, whole genome shotgun sequence.
ACCESSION   DATACA010000530 DATACA010000000
VERSION     DATACA010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395168"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VHK28_10710"
     CDS             complement(<1..587)
                     /locus_tag="VHK28_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX2142721.1"
                     /translation="MDAVPYLAALSAIAGLVLAAYFYGIVKKAEPGNARMVQLMEAIQ
                     AGSRAFLRREYTWVAGFVVLMTVLIAAFLDYGRPWGAIAYISGAVLSGLTGFVGMTVA
                     TLANARTANAAQVGGAKVALPLAFRGGAVMGFTVAGLALLGLAVSYLVYVEWLEVDDP
                     FSVLTAFGLGASSIALFSRVGGGIYTKAADVGADLV"
     gene            complement(705..>1605)
                     /locus_tag="VHK28_10715"
     CDS             complement(705..>1605)
                     /locus_tag="VHK28_10715"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX2142722.1"
                     /translation="VVAAVAPQWVPHPEVWLLVGGLVGLYAYAARVIGPKVVPAGVPS
                     VTRSQWRWFGGGIVLLWVAADWPVHDIGEQHLYLVHMAQHTVLTLLIPPMLLMATPEW
                     LARLVLGRGRVDHWVHTLARPIPAAIAFNALALLSHWQVVVNTASQNGLFHYGMHTAI
                     VVSAFLLWIPVCGPLPELRISMPAQMLYLFVTSIVPTVPGAWLVFAEGAVYSVYDIPD
                     RLWGVSVTSDQQVAGAIMKLGAGTYLWVIIAVLFFTWASRHAAAEQVPSVRAAGGTLT
                     WDQVQAELDRLPPGPEEARPSIE"
BASE COUNT          275 a          546 c          597 g          187 t
ORIGIN      
        1 acgaggtcgg cgccgacgtc ggccgccttg gtgtagatgc cgccgccgac acgtgagaac
       61 agggcgatgg agctggcccc gagcccgaac gccgtgagga cgctgaaggg atcgtcgacc
      121 tcgagccact cgacgtacac gaggtagctg accgccagcc ccagcagggc caggccggcc
      181 accgtgaacc ccatgacggc gccgccgcgg aaggcgagcg gcagggccac cttggcgccg
      241 ccgacctgcg cggcgttggc ggtgcgggcg ttggcgagcg tggcgaccgt catgccgacg
      301 aagccggtca ggcccgagag caccgcaccc gagatgtagg cgatggcgcc ccacgggcgg
      361 ccgtagtcga ggaaggcggc gatgagcacc gtcatgagca cgacgaagcc ggcgacccag
      421 gtgtactccc ggcggaggaa ggcgcgggac ccggcctgga tggcctccat gagctgcacc
      481 atgcgtgcgt tgcctggctc cgccttcttg acgatgccgt agaagtaggc ggcgagcacg
      541 aggccggcga tggcgctcag ggcggcgagg tacgggacgg cgtccacggg tggtggtcct
      601 ctcgttgacg tcagggatcg atgcgggcgc ggcggacccg gcccagccgc aggaatgtag
      661 cgatcaggtt cgcgatgact caaaaccgag gtcgcgagga cctgtcactc gatgctcggg
      721 cgcgcctctt cggggcccgg cggcagccgg tcgagctccg cctgcacctg gtcccaggtg
      781 agcgtcccgc cggcagcccg cacggaaggc acctgctccg cggccgcgtg gcggctggcc
      841 caggtgaaga agaggaccgc gatgatcacc cacaggtagg tccccgcacc gagcttcatg
      901 atcgcacccg cgacctgctg gtccgacgtg acggacaccc cccacaggcg gtcggggatg
      961 tcgtacacgg agtagaccgc gccctcggcg aacaccagcc aggcgccggg cacggtgggc
     1021 acgatggagg tcacgaagag gtagagcatc tgggcgggca tggagatccg cagctcgggg
     1081 agcgggccgc acaccgggat ccagagcagg aaggcgctca cgacgatggc cgtgtgcatg
     1141 ccgtagtgga agagcccgtt ctgcgacgcc gtgttgacga cgacctgcca gtgcgacagc
     1201 agcgcgagcg cgttgaaggc gatcgcggcg gggatcgggc gggccagcgt gtgcacccag
     1261 tggtccaccc ggccccgtcc cagcacgagg cgggccagcc actcaggcgt cgccatgagc
     1321 agcatcggcg ggatgagcag cgtcagcacc gtgtgctgcg ccatgtggac caggtagagg
     1381 tgctgctcgc cgatgtcgtg gaccggccag tcggccgcaa cccacagcag cacgatcccg
     1441 ccgccgaacc agcgccactg cgagcgggtg acgctcggca cgccggccgg cacgaccttc
     1501 gggccgatca cccgggcggc gtacgcgtag agcccgacca gcccccccac gagcagccac
     1561 acctcggggt gggggaccca ctgcggcgcg acggcggcca ccacg
//
LOCUS       DATACA010000531         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1842116, whole genome shotgun sequence.
ACCESSION   DATACA010000531 DATACA010000000
VERSION     DATACA010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842116"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VHK28_10720"
     CDS             complement(<1..571)
                     /locus_tag="VHK28_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX2142723.1"
                     /translation="MTQPSVALRAMALAAALVLVACTSPTAEPATSDGVAAPEVSELT
                     VGILPIVDVAPVHIAISEGLFEAEGLTVRTEVVQGGAAAIPALQGGDLDITYGNWPSF
                     LLANQEGIDLVAVADGVAATPGFMEFMALPASGLGGNPEGLAGAIIAVNTLNNVGELA
                     VRSTLRAVNIEFDSVQLVEIPFPDMAATLE"
     gene            677..1156
                     /locus_tag="VHK28_10725"
     CDS             677..1156
                     /locus_tag="VHK28_10725"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2142724.1"
                     /translation="MSSSSFAADVDRRAPVIESAEVSVAADRGTVWAVMSDIEAWPSW
                     SKDISSAELLGPLAPGTEFVWQAGPGTIRSRLVAVEPGSRIGWTGSTMGIRAVHQWRI
                     EEQGDRTRVVTDESWSGLLPWLLRAPMRSRLRTSLREGLTYLQAEAERRATLARGSS"
     gene            1153..1512
                     /locus_tag="VHK28_10730"
     CDS             1153..1512
                     /locus_tag="VHK28_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HEX2142725.1"
                     /translation="MSQNAAVLGLHFMLEVAALAAMAYWGWTRQDGATRWLLATGLPI
                     LAATAWAVFRAPNDGPQALVAIPGFARLALELLVLGGAALLLWQAAQPALAAGFAALI
                     VIDYLLQYDRVGRLLGL"
     gene            complement(1519..>2114)
                     /locus_tag="VHK28_10735"
     CDS             complement(1519..>2114)
                     /locus_tag="VHK28_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX2142726.1"
                     /translation="PEHEALVGDSVGLALLVVLDTLSPPERIAFVLHDVFGMPFVEIA
                     QVVDRSPAAARQLASRARRRVRGAPVPDPDIKAQRAVADAFLAAARKGDFEGLLSLLD
                     PDVVLRSDGGKARPGLVRVVRGAEAVASRAMEFSRFGESATPVLVNGVAGAVAWTPKG
                     EPFAVGSFTVRDGKIVSMEILADPDRIRQLDLTILVD"
BASE COUNT          315 a          731 c          750 g          318 t
ORIGIN      
        1 gctcgagggt ggccgccatg tcgggaaagg gaatctcgac gagctgcacc gagtcgaact
       61 cgatattcac cgcgcgcagc gtggagcgca ccgccagctc gcccacgttg ttcagcgtgt
      121 tgaccgcgat gatggcgcca gccaggcctt cggggtttcc gcccagcccg gaggcgggaa
      181 gggccatgaa ttccatgaag ccgggtgtcg ccgccacccc gtcggcgacg gcgaccaggt
      241 cgattccctc ctggtttgcc agcaggaacg acggccagtt gccgtaggtg atgtccaggt
      301 cgccgccctg cagagcgggg atggccgccg cgccgccctg caccacctcg gtcctgaccg
      361 tgaggccctc ggcctcgaac agcccctcgg agatggcgat gtgtaccggc gccacgtcga
      421 cgatgggcag gatgccaacc gtcagctcgc tgacctctgg agccgcgacg ccgtcggagg
      481 tggcgggttc agcagttggt gacgtgcaag cgaccaggac taaagcggct gccagcgcca
      541 tggcgcgaag ggccacgctg ggttgggtca tggtcgatcc cggcctgcct ggagtggagt
      601 ccaacggtgc ggatgctagt tgaaacctcg ccaggcgcgg gtggatggct cggggcccag
      661 ccctagacta gtgctcatgt cgtcttcgtc gtttgcagca gatgtcgacc gccgcgcccc
      721 ggtcatcgag agcgccgagg tcagcgtcgc cgccgaccgc gggacggtct gggcggtcat
      781 gagcgatatc gaggcctggc catcgtggag caaggacatt tcctcggccg agctgctcgg
      841 tccgctcgcg cccggaaccg agttcgtgtg gcaggctgga cccgggacca tccgctcgag
      901 gctggtcgcg gtcgagccgg gcagccggat cggctggacc ggcagcacca tgggcatccg
      961 cgccgtccac cagtggcgga tcgaggagca gggcgaccgg acacgggtcg ttaccgacga
     1021 gtcctggagc gggctgctgc cctggctcct gcgcgcaccg atgcgcagca ggctccgcac
     1081 ctcgctccgc gaaggcctca cctacctcca ggccgaagcc gagcgacgtg ccaccctcgc
     1141 acgaggatcg tcgtgagcca gaacgcggcg gtccttgggc tccacttcat gctggaggta
     1201 gcggcgctgg ccgcgatggc ctactggggc tggactcgcc aggatggggc cacccgctgg
     1261 ctgctggcca cggggctgcc gatcctggcc gccacggcct gggcggtctt tcgagcgccc
     1321 aatgacggac ctcaggccct ggtggccatc cccgggttcg cgcgtttggc gctcgagctc
     1381 ctcgtgctcg gcggcgcggc cctcctgctg tggcaggcgg cgcagccggc gctggctgcc
     1441 ggcttcgccg ccctgatcgt gatcgactac ctcctgcagt acgaccgggt ggggcggctg
     1501 ctgggcctct aggttggatc agtcgacgag gatcgtgagg tccaactggc ggatacgatc
     1561 ggggtcggcc aggatctcca tggacacgat ctttccgtcg cgaaccgtga agctgccaac
     1621 ggcgaacggc tcgcccttcg gcgtccaggc caccgccccg gccactccgt tgaccagcac
     1681 cggcgtggcg gattcgccga agcggctgaa ctccatcgct ctcgaggcaa cggcctcggc
     1741 gcctctcacc acgcgcacca ggcccgggcg cgccttgccg ccgtcggagc ggagcaccac
     1801 gtcgggatcc agcagggaca gcaggccctc gaagtcgccc ttccgggccg cggccaggaa
     1861 cgcgtccgcc accgcgcgct gggccttgat gtccggatcc gggaccggcg cacctcgaac
     1921 gcggcgcctg gcacggctgg cgagctgacg cgccgcggcg ggggagcggt ctacgacctg
     1981 cgcgatctca acgaagggca tgccgaacac gtcgtgcagc acgaacgcga tgcgctccgg
     2041 cggcgagagg gtatcgagca ccacgagcag cgccaggccc acggagtcgc ccaccagcgc
     2101 ttcgtgctcg gggt
//
LOCUS       DATACA010000532         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_636688, whole genome shotgun sequence.
ACCESSION   DATACA010000532 DATACA010000000
VERSION     DATACA010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636688"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VHK28_10740"
     CDS             complement(<1..388)
                     /locus_tag="VHK28_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142727.1"
                     /translation="MPASLPPWALLLLLAAVLLAAGLLAIRASGARFGLARRLAGAGE
                     VRVGDAFDLDPLPGRPVRLVGRIRCPDPIVTDRDDRLVALHRDVEVRLPGRGWRSIER
                     VRETRGFELWDHDGSLPLDPAAAAEPL"
     gene            532..1566
                     /locus_tag="VHK28_10745"
     CDS             532..1566
                     /locus_tag="VHK28_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142728.1"
                     /translation="MSASVRARARGPSGSRRALRRAAVLLVLSTSLLASLAIGAVSAL
                     PPAPVARLTAEEARAAGVGMSLVERADLAGGLLELRVDAAGAAPELLAVAPGGARAAT
                     APALGLDPTTLSVVHPDGSHLNVNLDGVVGAAFAPDSETLLVVDGGGTLQRIGLAAGT
                     LAVVADGPFLGPLAVDEDGSVLLLAVSSVEAPFRSRLVRLDPTSGEAKQLAEDDLVYG
                     AQELTDGSLAVVAHQRGGGTVVRRVADGVITLLADLGAGAVNVAVARDGRTLAWERQG
                     EGVFVADDPGARPRRLGPGSRPTFAPDGASLLVRDEDGGHVLVTLAGSRTRLSGPAAF
                     GASCTGRCRS"
     gene            1563..>2100
                     /locus_tag="VHK28_10750"
     CDS             1563..>2100
                     /locus_tag="VHK28_10750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2142729.1"
                     /translation="MRRLRFMGWAATLIAAAAVWLAAASVAAATDDYTLPFYDPNVGL
                     SYGMDRDWRLRVQLDWTGKVWWDSVPHWGRVYDNHTGLDYPMALRSTIAAARAGTVID
                     VEGGFGTQQKGQFGNFVLMSHSDGRRTLYYHLASAAEGGIHHGVGKQLAAGASVGLSG
                     CSGICSGPHLHFEVLLVSG"
BASE COUNT          270 a          743 c          787 g          300 t
ORIGIN      
        1 ccagcggctc cgccgccgca gccggatcca ggggcaggga gccgtcgtgg tcccacagct
       61 cgaagccgcg cgtctcgcgc acccgttcga tgctgcgcca gcccctgccc ggaaggcgca
      121 cctccacgtc gcggtggagc gccaccagcc tgtcgtcccg atcggtgacg atcggatccg
      181 ggcagcggat gcggccgacc aggcggactg gccgcccggg caacggatcg aggtcgaacg
      241 cgtctccgac tcggacctca ccggccccgg ccaggcgtct ggcaagcccg aacctggcgc
      301 cggaggcgcg gatcgcgagc aggccggcgg ccagcagcac ggcggcgagc aggaggagca
      361 gggcccaggg cggtagggag gcaggcatgg gcgccgatta tcgcctgata agcgtctgat
      421 aagcggtcgg cctctaggct gcagccccac gtctgacagg agggaggttc gccgccaacg
      481 gagcatccgt cgctcgcaat cgtccccggc ctgctcgcca cggagattcc catgtcggct
      541 tcggtgcgcg cccgtgcgcg cggtccatcc ggcagtcgcc gcgcgctgcg tcgtgccgcg
      601 gtgctgctgg tcctgtccac ctccctgctc gcctcgctcg ccatcggggc cgtgtcggcc
      661 ctgccgcccg cgccggtcgc gcgactgacc gccgaggagg cgcgcgcggc gggcgtcggc
      721 atgtcactgg tcgagcgtgc cgacctggcc ggcggcctgc tcgagctgcg cgtggacgcg
      781 gccggagcgg cgcccgagct gctggcggtg gcccccggcg gcgccagagc ggccaccgcc
      841 cccgccctcg gcctggatcc gaccacgctc agcgtggttc atcccgacgg gtcacacctc
      901 aatgtcaacc tggacggcgt ggtcggggca gctttcgccc cggacagcga gacgctgctg
      961 gtcgtggacg gcggcgggac gctgcagaga atcggcctgg cagccggcac gctcgccgtg
     1021 gtcgcggacg gacccttcct gggcccgctc gcggtcgacg aggacggcag tgtgctgctc
     1081 ctggccgtct cgtcggtcga ggccccgttc cggtcgcgcc tggtccgcct cgacccgacc
     1141 tcgggtgagg ccaagcagct ggcggaagac gacctggtat acggggcaca ggagctgacc
     1201 gatggttcgc tggcggtggt cgcccaccag cgcggcggag ggaccgtcgt ccggcgggtg
     1261 gccgacggcg tgatcacgct gctcgccgac ctgggcgccg gtgccgtgaa cgtagcggtc
     1321 gcgcgcgatg gacgaacgct cgcctgggaa cgccagggcg agggcgtctt cgtggccgat
     1381 gacccgggtg ccaggccgcg ccggctcggt ccgggctcac ggccgacgtt cgcgccggac
     1441 ggggcctcgc tgctcgtccg agacgaggat ggcggccacg tcctggtcac gctggctggg
     1501 tcacgcaccc ggttgtccgg ccccgcggcg ttcggcgcat cgtgcaccgg gaggtgccgg
     1561 tcatgaggcg gctgcgtttc atgggttggg ccgcgacgct catcgccgcg gcggccgtgt
     1621 ggctggcggc agcgtcggta gctgctgcaa cggacgatta caccctcccg ttctacgacc
     1681 cgaacgtcgg cctcagctac ggcatggacc gcgattggcg gctgagggtg cagctggact
     1741 ggaccggcaa ggtgtggtgg gactcggtcc cgcactgggg acgggtgtac gacaaccaca
     1801 ctggcctgga ctatcccatg gcccttcgct cgaccatcgc cgccgccagg gcaggcacgg
     1861 tgatcgacgt ggagggcggg ttcggcacgc agcagaaggg ccagttcggc aacttcgtgc
     1921 tgatgagcca ttctgacggg cggcggaccc tctactacca cctggccagc gctgccgagg
     1981 gcggcatcca ccacggcgtc ggcaagcagc tggcggccgg tgccagcgtg gggctgtccg
     2041 ggtgcagcgg catctgcagc gggccccacc ttcacttcga ggtgctgctg gtcagcggcc
//
LOCUS       DATACA010000533         7287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_958800, whole genome shotgun sequence.
ACCESSION   DATACA010000533 DATACA010000000
VERSION     DATACA010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958800"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1054
                     /gene="rpsB"
                     /locus_tag="VHK28_10755"
     CDS             143..1054
                     /gene="rpsB"
                     /locus_tag="VHK28_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2142730.1"
                     /translation="MAATSMKQLLEAGVHFGHQTRRWNPKMRPFIFAERNGIHIIDLA
                     QTVDRLEAALAFVRATVARGDSVLFVGTKKQAQEAVALEAERSGQHFVNTRWLGGMLT
                     NFQTIKRRIQRLEALEARRDSGEFERLTKKEASKLNEEITKLNAALGGIRKMRRLPGA
                     VFVVDPHRETIAVAEARRLEIPIVAMTDTNCDPDLIDWVIPANDDAIRSVRLVTAKVA
                     DAVLEAMAERQARLDEEFEQAEALPPVDESVLAVDSDITAALAAGEALIFEPEPDEDE
                     EEERRARARRATETAEGSEEEETVESE"
     gene            1114..1743
                     /gene="tsf"
                     /locus_tag="VHK28_10760"
     CDS             1114..1743
                     /gene="tsf"
                     /locus_tag="VHK28_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX2142731.1"
                     /translation="MSTTINPQDVKRLREQTGAGVMDCKRALEESGGDVDAAIAWLRE
                     KGLSTAAKKAGRSVREGVIASYIHHGSRLGVLLELNCETDFVARTDDFQQLARELAMQ
                     VAGLSPLYVSREDVPAAVLEDRQRVFSEEAEREGRPSDRVPQIVEGKLSKWFETVCLL
                     EMPYRDTDKKVSDLITEKIALLGENIRVARFARMAVGETAGEAGAEEAA"
     gene            1745..2491
                     /gene="pyrH"
                     /locus_tag="VHK28_10765"
     CDS             1745..2491
                     /gene="pyrH"
                     /locus_tag="VHK28_10765"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2142732.1"
                     /translation="MSQAAVPDQPRYRRVLLKLSGEALMGTRQYGIDPDVVRGIAAQV
                     HEARLADIEMAVVVGGGNIFRGLSASASGMDRSTADYMGMLATVMNGLAIQDALEQSG
                     TPTRVLSAIAMPEICEPYIRRRAVRHLEKGRVVVLVAGTGNPYFTTDTAATLRAVEMG
                     AEVILKATRVDGVYDADPEKDPAATRYARISYTDLISNRLEALDATAVSLAMDNEMPI
                     VVFDMTQPGNIVRAVRGEPIGTLIAGEVQT"
     gene            2488..3045
                     /gene="frr"
                     /locus_tag="VHK28_10770"
     CDS             2488..3045
                     /gene="frr"
                     /locus_tag="VHK28_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX2142733.1"
                     /translation="MTHEALVAAEPKMQRAVEAMERDFAGVRTGRASTALVERIPVDY
                     YGTPTPLNQIAGISTPDPHLIVIQPWDRSVLSAIEKAITRSDIGLTPNVDGTVVRLNV
                     PPLTEERRRDMVKQVRRRAEEARVEVRNHRREAADQLKRALKDGDLSEDEERRELDSL
                     QRLTDRFVEAIDGRAERKEAEVMEV"
     gene            3042..3737
                     /gene="uppS"
                     /locus_tag="VHK28_10775"
     CDS             3042..3737
                     /gene="uppS"
                     /locus_tag="VHK28_10775"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2142734.1"
                     /translation="MTRAEARPRHVAIICDGNRRWARARGLPVIAGHVQGVEAIRPVV
                     RRARDRGVETLSVYTFSTENWSRPDDEVTGLFGLIDGAIRQHTDELVKEGVRVHVIGR
                     LHEAPSDVQRSIRSAEERTAGGEQLVLNICFNYSGRAEVIDAVRAMVATGIDPAHIDE
                     ERLSGFMYTAGQPDVDLLIRTGGDQRTSNFLIWQATYAELVFSPKMWPDFSPDDFDAA
                     LAEYAGRERRFGA"
     gene            3741..4640
                     /locus_tag="VHK28_10780"
     CDS             3741..4640
                     /locus_tag="VHK28_10780"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX2142735.1"
                     /translation="MLRTRLVSAVVLAPLVLAVVFLGEPWLSLLVGVIAFLALVEVIA
                     LLDAAGFEPPQVLALALGTVLAATVLVAANRALVGGLLAELLRVTTVPGLPIVAFVAA
                     VLLLAVASFARAEPRAGFLTWATTSFGVAYAGLLAPFIVLVAHLGPVGGSAETQVGAL
                     SLRAGSAWALLLILTVWGYDTGAYLTGRWIGRRRLIDHISPSKTVEGLAGGLLAATLA
                     AGLGAWLVGLPAWQPLVIGPLLGLAAQAGDLAESLLKRAAGRKESGFLIPGHGGMLDR
                     IDSFLFAAPTLAGFALLAGGAAL"
     gene            4647..5813
                     /gene="dxr"
                     /locus_tag="VHK28_10785"
     CDS             4647..5813
                     /gene="dxr"
                     /locus_tag="VHK28_10785"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX2142736.1"
                     /translation="MSTGVAILGSTGSIGRQALEVIAAHPGRFRVVGLAAAHPSETFI
                     GQLADWSEATAWCAAGVPEGLDARRWASGGLAEIAMLDGADLVVVATTGMTALPAVLA
                     ALRAGRRVALANKETLVTGGHLVAAALEELAPGTDPLEQLDRLRPIDSEHSAIWQSLR
                     GERLADVRRLVLTASGGPFLRTAPDRLARVTREEALAHPTWRMGPKITIDSATLVNKA
                     FEVIEAKWLYRLPYSTIAAVIHPGSVVHSLVEFVDGSFKAQLGVPDMRLPIQYALTYP
                     QRLPSPARHTPPEAWDALAFEPLDESRYPAYTVTRRAAEAGGNRGSVLNAADEVAVAA
                     FLDGRIGFDKISTVIESAVDRWGTDVEPGLDAIAELDSEVRLVMGAELGMRGDA"
     gene            5813..6934
                     /locus_tag="VHK28_10790"
     CDS             5813..6934
                     /locus_tag="VHK28_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX2142737.1"
                     /translation="MAVIGTVAAFLAVLVVLVLIHELGHFIAAKRAGITVQEFGVGFP
                     PRIASVVWRGTRYSVNWIPLGGFVKMLGEDGELEAEKMRERGLSDAAIDKAMAGAFNR
                     KPIWVRIVVLVAGVAMNFLLAGILFAVALSLPVPEGRGPLEVVAIQEDSPAASADLRV
                     DDVIVAADGRSYEVSRELTEYVRSRAGEPVVLDVLRDGQEHQITVTPRDPDELRPGEG
                     PIGFTYQPSRYVEVPSEVQGPVDALVSGFSEAGYLAYRIPGGLAEAVGGLLGLNPNAG
                     QAVGPIGIAEETGRVLQAPLVSQLVFMGVLSVNLAVLNILPFPPLDGGRVMVVLIEAL
                     RRRRLPAEREALIYLTGFMVLIALVILISIQDVQRLIEG"
BASE COUNT         1094 a         2451 c         2668 g         1074 t
ORIGIN      
        1 ttggtccggc acccgccgga cgtcattcca tcacacacgc tcgcggattc cgcggcccgc
       61 ggtgcccgca cgtgcctgac aggcgcgtcg ggtgggcaga gggaagtcga agcgagcgga
      121 ggaacaaccg ggaggtaccc ttttggctgc cacgagcatg aagcagctgc tcgaggcggg
      181 cgtccacttc ggacatcaga cccgccgctg gaatcccaag atgcgcccgt tcatcttcgc
      241 cgagcgcaac gggatccaca tcatcgatct ggcccagacc gtggaccgcc tggaggcggc
      301 tctcgccttc gtgcgtgcca cggtggcacg cggcgacagc gtcctcttcg tcggcaccaa
      361 gaagcaggcg caggaggcgg tcgccctcga ggcggagcgt tccggccagc acttcgtgaa
      421 cacccgatgg ctgggcggaa tgctgaccaa cttccagacc atcaagcgcc gcatccagcg
      481 cctcgaggcg ctcgaggcgc gacgcgacag cggggagttc gagcgcctca ccaagaagga
      541 ggctagcaag ctcaacgagg agatcaccaa gctgaatgcg gccctcgggg gcattcgcaa
      601 gatgcgccgc ctgccgggcg cggtgttcgt cgtggacccg caccgcgaga cgatcgcggt
      661 agccgaagcc aggcgactgg agatccccat cgtggccatg accgatacca actgcgaccc
      721 ggacctgatc gactgggtca tccccgccaa cgacgacgcc atccgctccg tgcggctggt
      781 gacggccaag gtggccgatg cggtcctgga ggccatggcc gagcgccagg cccgcctgga
      841 cgaggagttc gagcaggccg aggcgctgcc gcccgtcgac gagtcggtgc tcgccgtcga
      901 ctccgacatc accgctgcgc tggccgccgg cgaggccctc atcttcgagc cagagccgga
      961 cgaggacgag gaggaggagc gccgggcgcg cgcgcgccgg gccaccgaaa ccgccgaggg
     1021 cagcgaggaa gaggagaccg tcgagtcgga gtgatggcgc gccgcgtgcg gcgccatcgg
     1081 tccagacagc gaccaaaccc ggaaggaaga cacgtgagta ccaccatcaa cccgcaggac
     1141 gtgaagcggc tccgcgagca gaccggagcg ggcgtcatgg actgcaagcg agccctggag
     1201 gagagcggcg gcgacgtgga tgccgccatc gcctggcttc gcgagaaggg gctgtccacc
     1261 gcagccaaga aggccggtcg cagcgtccgc gagggcgtga tcgccagcta catccatcac
     1321 ggctcgcgcc tgggcgtcct gctggagctc aactgcgaga ccgatttcgt ggctcgcacc
     1381 gacgatttcc agcagctggc ccgcgagctg gccatgcagg tggccggcct ctcaccgctg
     1441 tacgtatcgc gcgaggatgt cccggccgcc gtgctggagg atcggcagcg cgtgttcagc
     1501 gaggaggcag agcgcgaggg gcggccatcg gaccgcgtcc cgcagatcgt cgagggcaag
     1561 ctgagcaagt ggttcgagac cgtctgcctg ctcgagatgc cgtaccgcga cacggacaag
     1621 aaggtcagcg acctgatcac cgagaagatc gcgttgctgg gagagaacat ccgcgtcgcc
     1681 cgcttcgccc ggatggcggt gggggagacc gcgggggagg ccggcgccga ggaggctgcc
     1741 tgacgtgagc caggcggccg tcccagacca accgcgctac cgccgggtgc tgctcaagct
     1801 ctccggggag gcgctgatgg ggacgcgcca gtacgggatc gatcccgacg tggtccgcgg
     1861 gatcgcggcc caggtgcacg aggcgcgcct ggccgacatc gagatggccg tggtggtcgg
     1921 tggcgggaac atcttccgcg gcctgtccgc ctctgccagc gggatggacc gatcgaccgc
     1981 cgactacatg gggatgctgg ccaccgtgat gaacgggctg gccatccagg acgcgctgga
     2041 gcagagcggc acgccgaccc gcgtcctctc ggcgatcgcc atgccggaga tctgcgagcc
     2101 ctacatccgg cggcgggcgg tgcggcacct ggagaagggc cgcgtggtgg tcctggtggc
     2161 cggcaccggc aacccgtact tcaccacgga cactgctgcc acgctgcggg cggtcgagat
     2221 gggcgccgaa gtgatcctca aggcgacccg ggtggacggg gtgtacgatg ccgatccgga
     2281 gaaggaccct gccgccaccc gctacgcgcg gatcagctac acggacctca tctcgaaccg
     2341 gctggaggcc ctggacgcca cagccgtaag cctcgcgatg gacaatgaga tgccgatcgt
     2401 cgtcttcgac atgacccagc cggggaacat cgtgcgcgcg gtgcgcggcg agccgatcgg
     2461 gacgctgatc gccggggagg tgcagacatg acccacgagg cgctggtcgc cgcagagccc
     2521 aagatgcagc gggcggtgga ggccatggaa cgcgatttcg ccggagtgcg caccggccgc
     2581 gcctccaccg ccctggtgga gcgcatcccg gtcgactact acggcacgcc cacgccgctc
     2641 aaccagattg cgggcatcag cacaccggac ccgcacctga tcgtgatcca gccgtgggac
     2701 cgatcggtgc tgtcggccat tgagaaggca atcacccgca gtgacatcgg gctgaccccg
     2761 aacgtggatg gcaccgtggt gcgcctcaac gttccgccgc tgaccgagga gcggcggcgg
     2821 gacatggtga agcaggtccg gcgccgcgcc gaggaggcgc gggtggaggt tcgcaaccat
     2881 cgccgggagg cggcggacca gctcaagcgc gccctgaagg acggcgacct gtccgaggac
     2941 gaagagcgac gcgagctgga ctcgctgcag cgcctgaccg accgtttcgt ggaggccatc
     3001 gatggccgtg cggagcggaa ggaggcggag gtgatggaag tctgacccgg gcggaggcgc
     3061 gcccgcggca cgtcgccatc atctgtgacg gcaaccgtcg ctgggcgcgc gcccgcggcc
     3121 tgccggtcat cgccggacac gtccaggggg tggaagccat ccgtcccgtc gtgcggcgcg
     3181 cgcgcgacag gggggtggag acgctatcgg tctacacctt cagcaccgag aactggagcc
     3241 ggccggacga cgaggtgacc gggctgttcg ggctcatcga cggagccatc cggcagcata
     3301 ccgacgagct ggtgaaggag ggcgtgcggg tgcacgtgat cgggcgcctg cacgaggcgc
     3361 cgagcgacgt ccagcgctcg atccgttccg ccgaggagcg cacggcgggc ggggaacagc
     3421 tcgtgctgaa catctgcttc aactactccg gccgggcgga ggtgatcgat gccgtgcggg
     3481 ccatggtggc gaccgggatc gacccggcgc acatcgacga ggagcggctg tccggcttca
     3541 tgtacacggc cggccagccg gacgtggacc tcctgatccg caccggtggc gaccagcgca
     3601 ccagcaactt cctgatctgg caggccacct acgccgagct cgtcttctcg cccaagatgt
     3661 ggcccgattt cagccccgac gacttcgacg ccgcactggc cgagtacgcc ggccgcgagc
     3721 gtcgcttcgg cgcctgaccg atgctccgaa cgcggctggt ctcggcggtc gtcctggcgc
     3781 ccctcgtcct ggcggtcgtc ttcctgggcg agccgtggct ttcgctgctg gttggggtga
     3841 tcgccttcct ggcgctggtg gaggtcatcg ccctgctcga cgccgctggc ttcgagcccc
     3901 cgcaagtgct ggccctggcg ctgggaacgg tgctcgcggc gaccgtgctg gtggcggcca
     3961 accgtgcgct ggtcggcggg ctgctggccg agctgctgcg ggtcacgacc gtgccgggcc
     4021 tgccgatcgt ggccttcgtg gccgccgtgc tgctgctggc ggtggccagc ttcgcgcgtg
     4081 ccgagccacg cgccggcttc ctcacctggg cgacgacatc attcggcgtg gcctacgcag
     4141 ggctgctggc cccgttcatc gtgctggtgg ctcacctggg gccggtcggc ggctcggccg
     4201 agacgcaggt cggcgcgctg agcctgcggg ccgggtcggc ctgggcgctg ctgctgatcc
     4261 tcaccgtgtg gggctacgac acgggtgcct acctgaccgg tcgctggatc gggcggcggc
     4321 ggctcatcga tcacatcagc ccgtccaaga cggtcgaggg gctggccggc gggctgctgg
     4381 ccgcgacgct ggccgcgggc ctgggtgcct ggctggtggg gctgcccgcc tggcagccgc
     4441 tggtgatcgg gccgctgctc gggctggcgg cccaggccgg cgacctggcg gagtcgctcc
     4501 tcaagcgggc ggccggtcga aaggagtccg ggttcctgat tcccgggcac ggcggcatgc
     4561 tcgatcgcat cgactcgttc ctgttcgcgg cgcccacgct ggccggcttc gccctgctgg
     4621 ccggcggcgc cgcgctgtag ggtgacgtga gcactggcgt tgcgatcctg ggctcgaccg
     4681 gctccatcgg gcgccaggcg ctggaggtga tcgccgccca tcccggacgc ttccgggtcg
     4741 tgggattggc cgccgcccac ccctcggaga ccttcatcgg ccagctggcg gactggtccg
     4801 aggcgacggc atggtgcgca gccggcgtgc cggaggggct ggatgcgagg cgctgggctt
     4861 ccggtgggct ggccgagatc gccatgctgg acggcgccga cctggtggtc gtggccacca
     4921 ccgggatgac tgccctcccg gcggtgctgg ccgcgctgcg ggccggccgg cgagtggcgc
     4981 tggccaacaa ggagacgctc gtgaccggcg gccacctggt ggctgcggcc ctcgaggagc
     5041 tcgcgcctgg gaccgatccg ctcgagcagc tggaccggct gcggcccatc gacagcgagc
     5101 actcggccat ctggcagagc ctgcgcgggg aacgattggc cgacgtgcgg cgtctggtcc
     5161 tcaccgcctc aggcggacca ttcctgcgta ccgcacccga tcggctggca cgggtcaccc
     5221 gcgaggaggc gctggcgcat ccgacctggc gtatgggccc caagatcacg attgattcgg
     5281 cgaccctggt gaacaaggca ttcgaggtca tcgaggccaa atggctgtac cgccttccgt
     5341 acagtacgat tgcggctgtg atccatccgg ggagcgtcgt gcactcgctc gtcgagttcg
     5401 tcgacggctc cttcaaagcc cagctcggtg tccctgacat gcggcttccc atccagtacg
     5461 ccctgaccta tccccagcgc ctgccgtcgc ccgcacgcca tacgccgccg gaggcgtggg
     5521 acgcgctggc tttcgagccg ctcgacgagt cgcgctaccc cgcgtacacc gtcactcgac
     5581 gggccgccga agcgggcggg aaccgcggga gcgtgctcaa cgccgccgac gaggtggcgg
     5641 ttgctgcctt cctggacgga cgcatcggtt tcgacaagat ctcgaccgtg atcgagtcgg
     5701 cagtcgaccg atgggggacc gatgtggagc ccgggctgga cgccatcgcg gagctggaca
     5761 gcgaggtgcg gttggtaatg ggcgcggagc tcggcatgcg gggtgacgcc tgatggccgt
     5821 gatcgggacg gtcgccgcct tcctggccgt cctggtcgtg ctggtcctca tccacgagct
     5881 gggccacttc atcgccgcca agcgagccgg catcaccgtc caggagttcg gcgtcggctt
     5941 cccgccgcgc atcgcctcgg tggtgtggag aggcacgcgc tactcggtca actggatccc
     6001 gctgggtggc ttcgtgaaga tgctcggcga ggacggggag ctggaggccg agaagatgcg
     6061 cgagcgcggc ctctcggacg cggccatcga caaggccatg gccggcgcct tcaaccgcaa
     6121 gccgatctgg gtgcgcatcg tagtgctggt ggccggcgtg gccatgaact tcctgctggc
     6181 cggcatcctc ttcgcggtcg cgctcagcct cccggtgccg gaggggcgcg gcccgctgga
     6241 ggtggtcgcc atccaggagg acagcccggc cgcgagcgcc gatttgcgcg tggacgacgt
     6301 catcgtggcg gccgacggcc gcagctacga ggtctcgcgc gagctgaccg agtacgtccg
     6361 ctcccgcgcc ggcgagccgg tggtgctgga cgtgctgcgc gacgggcagg agcaccagat
     6421 cacggtcacg ccgcgagacc cggacgagct gcgccccggg gagggaccca tcggcttcac
     6481 ctaccagccc tcgcggtacg tggaggtgcc atccgaggtg cagggcccgg tggatgcgct
     6541 ggtgagcggc ttcagcgagg ccggctacct ggcctaccgc atacccggcg ggctggccga
     6601 ggcggtcggc gggctgctgg gcctcaaccc gaatgccggg caggcagtcg ggcccatcgg
     6661 catcgccgag gagacaggtc gcgtgctgca ggcgccgctg gtcagccagc tggtgttcat
     6721 gggcgtgctg tccgtcaacc tggcggtgct caacatcctg cccttcccgc cgctggacgg
     6781 tggccgggtg atggtggtgc tcatcgaggc gctgcggcgt cgccggctgc ccgcggaacg
     6841 cgaagcgctg atttacctga ccggcttcat ggtgctgatc gcgctcgtga tcctgatctc
     6901 catccaggac gtccagcgcc tcatcgaggg ctgatccgag cgcgaatgaa ggcccctggc
     6961 caaccgccag aggccctcgt acccctcgcc gtaccctatt acccgccgag cacggcgtcc
     7021 atgcgttgcg ccagccggtc ctgcatggcg ggcgtccagc ggccgtagat gtcggctgtc
     7081 gtggccaggc tggtgtgacc tagtgcgcgg agctgcacga cgtcctggtg gccagcctgc
     7141 caccggacct ccgcgacctg ctgaccttcg actattcgtg caccgtgcgt agggcgacgg
     7201 tggaggcgat gctggtgcgc gaaggcaacg cgcaaggtgt ccggcaaggg tgcaggacgc
     7261 tagcatgcga ccagatgccg ggccggc
//
LOCUS       DATACA010000534         5233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1119309, whole genome shotgun sequence.
ACCESSION   DATACA010000534 DATACA010000000
VERSION     DATACA010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119309"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VHK28_10795"
     CDS             <1..665
                     /locus_tag="VHK28_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142738.1"
                     /translation="HYFLEMNTRLQVEHGVTELVTGIDIVAWQIRIAAGERLPDEILQ
                     RRLDGHAIEVRLYAEDPYDAFRPVGGAVTTWRLPEGPGVRVDAAVKTGLPLTPEYDPL
                     LAKLMVHAADRPAAVRRLARALDETQVGGLQTDLGFHRWLVDEPGFRDGVYHTGLITE
                     HWGAAGPELTDDERQLAAVAAIHARTTQRTRQSGTAAPVPPAGITQSAWAAAARREAL
                     RR"
     gene            662..1129
                     /locus_tag="VHK28_10800"
     CDS             662..1129
                     /locus_tag="VHK28_10800"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2142739.1"
                     /translation="MNDELEVRVAGEAVEPGEGWTASSLDHATGLVRLTDGDRSRTVL
                     VEGAGSEWVVTHRGRRIPVTLRTWRERVLAEAEVAAQARGGPLDIKATLPGLVVAVVV
                     AEGVAVEEGDPLLTIEAMKMQNEVRAPRAGTVGAVAVAPGQPVPTGALLVRLE"
     gene            1189..2787
                     /locus_tag="VHK28_10805"
     CDS             1189..2787
                     /locus_tag="VHK28_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2142740.1"
                     /translation="MTDPEQTAHPPTRTTSGIEISDSYGPEALEGFDAASRLGAPGEF
                     PFTRGLRGDGYRGRLWTMRQYAGFGSAAQTNQRFRYLMERGQTGLSVAFDLPTQMGYD
                     SDAPQAVGEVGRVGVPIDTIEDMELLFDGIDLGRVSTSMTINATAAILLALYEAVGRR
                     QGVDPAKLRGTIQNDILKEYIARGTYIYPPEPSMRLVTDTFAYCQTELTAWNTISISG
                     YHMREAGATAVQEVAFTIANAIAYADAALRAGLEFDQFAPRLSFFFAAHNDLFEEVAK
                     FRVARRVWASVARDRFGATDSRSTAMRFHVQTGGSTLTAQQPETNVVRTTVQALAAVL
                     GGAQSLHTNALDEALGLPTPETARLALRTQQVLAHESGVASTADPLGGSYFIEWLSER
                     LEEGVWAELAEIDRRGGTLAALAEGYQPGEIADAAYAIQRELEAGERIVVGLNAFTDG
                     GEDQRPAPQEIDADDERRQVERTRAIRESRDQDLAQAALDALVTAARGTENVLPRIRA
                     CVEASVTLGEISDALRGAWGEHRP"
     gene            2784..3209
                     /locus_tag="VHK28_10810"
     CDS             2784..3209
                     /locus_tag="VHK28_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2142741.1"
                     /translation="MTVGGIHHLAVVVRSIEESVERYQDLFGWQPEAEPFTFDPQRVR
                     LCFLAAPDPSGSPRIELVQPTEAESGVGRFLATRGEGLHHVCLLSDDLPADLAALAAA
                     EAELIDREPRPGAHGRVAFVHPRTLNGVLWELLQTEPGR"
     gene            complement(3222..4100)
                     /gene="sucD"
                     /locus_tag="VHK28_10815"
     CDS             complement(3222..4100)
                     /gene="sucD"
                     /locus_tag="VHK28_10815"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX2142742.1"
                     /translation="MSILVNADTRLVVQGITGREGEFHSRAMLEYGTNVVAGVTPGKG
                     GQSALDGRVPVFDTVLDAVGETGANASCIFVPAPFAPDAVLEAADAGVPLVICITEGI
                     PALDMLTVYHVLRAQGVRLIGPNCPGATSPGQAKVGIIPGNIHRPGSVGLVSRSGTLT
                     YEVVQAMTDAGLGQSTCVGIGGDPIVGTSFIDVLDMFNQDPETDAIVMIGEIGGEEEE
                     KAAAFVKREVRKPMAAFIAGRTAPPGRRMGHAGAIISGGAGTAESKRTALVEAGIRVA
                     DSPLQIPALLREAGVS"
     gene            complement(4097..>5233)
                     /gene="sucC"
                     /locus_tag="VHK28_10820"
     CDS             complement(4097..>5233)
                     /gene="sucC"
                     /locus_tag="VHK28_10820"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX2142743.1"
                     /translation="KLQEYRSKEVLARHGVPLLDGEVATTPAEARAAAERIGGPVVVK
                     AQVLVGGRGKAGGVKLAATPEEAEQRAREIIGLDIKGVTVRTVLVAPAADIAKEYYLG
                     LILDRVARAVTVIASAEGGVEIEETARTNPDAILRLPLHPLLGLQPYNVRRTSFFLGI
                     PAELRRDFASVLQGLYEAFMGADADLAEINPLVITDDGRLLALDAKIVLDDSALFRHP
                     DLEAMRDLNEEEPSEIAAREAGINFIKLDGNIGCMVNGAGLAMTTMDLVKLAGGEPAN
                     FLDIGGGTKADRVLAAFRIILDDPNVHAILVNIFGGITRGDEVARGIVEARAGLEREV
                     PMVVRIVGTNAEEAAGILEQASLITAASLDEAAAKAVEAARSAS"
BASE COUNT          767 a         1867 c         1859 g          740 t
ORIGIN      
        1 accactactt cctggagatg aacacccgcc tgcaggtgga gcacggggtc actgagctgg
       61 tcaccggcat cgacatcgtg gcctggcaga tccggatcgc ggcgggggag cggctgccgg
      121 acgagattct ccagcgccgg ctcgacggac acgcgatcga ggtccgcctc tatgccgagg
      181 acccgtacga tgcgttccgc ccggtgggtg gggccgtcac cacctggcgc ctgccggaag
      241 ggccgggcgt tcgggtcgac gccgcggtca agacgggcct cccgctgacg cccgagtacg
      301 acccgctgct cgccaagctg atggtgcacg ccgccgaccg gcctgccgcc gtgcgccgtc
      361 tggcccgagc gctggacgag acacaggtgg gtggcctgca gacggacctg ggctttcacc
      421 gctggctggt cgatgagcca gggttccggg acggggtgta ccacacgggt ttgataaccg
      481 agcactgggg cgccgccggg cccgagctga ccgacgacga gcggcagctg gcggcggtgg
      541 ccgccatcca cgcgcgcacg acacaacgaa cccggcagtc cggcacggca gcacctgtgc
      601 cgccggcagg catcacgcag tcagcctggg cggccgccgc gcgccgcgag gcactccgcc
      661 gatgaatgac gagctcgagg ttcgcgtcgc cggcgaggca gtggaaccgg gcgagggctg
      721 gacggccagc tcgctcgacc acgcgacggg cctcgtccgg ctcaccgatg gcgaccgcag
      781 ccggacggtc ctggtggagg gcgcgggcag cgaatgggtg gtcactcacc gcggccgccg
      841 gatcccggtc acgctgcgca cctggcggga gcgggtgctg gccgaggcgg aggtcgccgc
      901 gcaggccaga ggcgggccgc tggacatcaa ggccactttg ccggggctgg tggtcgcggt
      961 cgtggttgcc gagggcgtcg cggtggagga gggtgacccg ttgctgacca tcgaggccat
     1021 gaagatgcag aacgaggtgc gcgcgccccg cgccggcacc gtcggggcgg tggcggtggc
     1081 gcccggccag ccggtcccca ccggcgcgct cctggtgcgg ctcgagtagc gccggtcggc
     1141 cgccggtttg gccatgggcc ccgggggcat cggtacaatc agccgtccat gaccgatccc
     1201 gagcaaaccg cccacccacc cacgcggacc acctcgggga tcgagatctc ggacagctac
     1261 ggccccgagg ccctggaggg tttcgatgcg gccagccgtc tgggcgcgcc gggcgagttc
     1321 ccgttcaccc gcggcctacg aggcgacggc taccgcggcc gcttgtggac aatgcgccag
     1381 tacgccggct tcggctccgc ggcgcagacc aaccagcgct ttcgctacct gatggagcgc
     1441 ggccagacgg gcctgtcggt ggccttcgac ctgccgaccc agatgggcta cgacagtgac
     1501 gcaccgcagg cggtcggcga ggtgggccgg gtgggcgtgc ccatcgacac catcgaggac
     1561 atggagctgc tcttcgacgg gatcgacctg ggacgggtga gcacctccat gaccatcaac
     1621 gccacggccg ccatcctgct ggcgctctac gaggcggttg gacgccgcca gggagtggac
     1681 ccggcaaagc tgcgcggcac gatccagaac gacatcctca aggagtacat cgcgcgcggg
     1741 acctacatct atccccccga gccctcgatg cgcctggtga ccgatacctt cgcctactgc
     1801 caaaccgagc tcacagcctg gaacacgatc agcatcagcg gctaccacat gcgcgaggcc
     1861 ggcgcgacgg cggtgcagga ggtggccttc accatcgcca atgccatcgc ctatgccgat
     1921 gccgccctgc gggcgggcct cgagttcgac cagttcgccc cgcgactgtc cttcttcttc
     1981 gcggcgcata acgacctgtt cgaggaagtg gccaagttcc gcgtggcccg gcgggtgtgg
     2041 gccagcgtcg cacgcgaccg cttcggcgcc accgactcgc gctcaacggc catgcgcttc
     2101 cacgtccaga ccggaggctc cacgctgacc gcccagcagc cagagaccaa cgttgtgcgg
     2161 accacggtgc aggccctggc cgccgtcctg ggaggtgcac agtcgctgca caccaatgcc
     2221 ctggacgagg cgctgggcct ccccaccccg gagaccgccc gcctggcgct tcgaactcaa
     2281 caagtgttgg ctcacgagtc cggcgtggcg tccacggctg acccgctggg cggctcgtac
     2341 ttcatcgagt ggttgagcga gcgcctggag gagggcgtct gggccgagct ggcggagatc
     2401 gaccggcgcg gcgggacgct ggccgcgctg gcggagggtt accagcccgg cgagatcgcg
     2461 gacgccgcct acgccatcca gcgcgagctc gaggcggggg agcggatcgt ggtggggctc
     2521 aacgccttca ccgacggagg cgaggaccaa cgcccggccc cgcaagagat cgacgccgat
     2581 gacgagcggc ggcaggtgga gcgcacgcgc gccattcgcg agtcacgcga ccaggacctg
     2641 gcgcaggccg cgctcgacgc gctggtgact gcggcgcgcg ggacggagaa cgtgctgcct
     2701 cgcatccgtg cctgcgtgga ggccagcgtc accctgggcg agatcagcga cgcgcttcgt
     2761 ggggcgtggg gcgagcaccg gccgtgaccg tgggtggcat ccatcacctg gcggtcgtgg
     2821 tgcgctccat cgaggaatcg gtcgagcgct accaggacct cttcggctgg caaccggagg
     2881 ccgagccgtt caccttcgac ccgcaacgcg tccggctctg ctttctggcc gccccggacc
     2941 cgtccggctc gccgcgcatc gagctggtgc agcccacgga ggccgaaagc ggcgtcggcc
     3001 gcttcctggc cactcgcggg gaggggctcc accacgtctg cctgctctct gacgacctgc
     3061 cggcagacct cgcggcgctg gcagccgccg aggcggagct gatcgaccgc gagccgcggc
     3121 ccggggcgca cggccgggtg gccttcgtcc atccgcggac gctcaacggc gtcctgtggg
     3181 agctgctgca gaccgagccg ggccgctgag cgagcctgct gtcagctgac gccggcctcg
     3241 cggagcaggg ccgggatctg cagcggggag tcggcgacgc ggatgcccgc ctccaccagg
     3301 gcggtccgct tggactcggc ggtaccggcc ccgccggaga tgatcgcgcc ggcatggccc
     3361 atccggcgcc ccggcggggc ggtgcgaccg gcgatgaagg cagccatcgg cttgcgcacc
     3421 tcgcgcttga cgaaagcggc cgccttctct tcctcctcgc cgccgatctc gccgatcatc
     3481 acgatggcat cggtctcggg gtcctggttg aacatgtcga gcacgtcgat gaagctggtg
     3541 cccacgatcg ggtcgccacc gatgccgacg caggtcgact ggcccaggcc ggcatcggtc
     3601 atggcctgca ccacctcgta ggtcagcgtc ccggaacggc tgaccaggcc gaccgagccg
     3661 ggccgatgga tgttgccggg gatgatgccg accttggcct gtcccgggga ggtggcgccg
     3721 ggacagttgg gcccgatcag gcgcacgccc tgcgcgcgca gcacgtggta cacggtgagc
     3781 atgtcgagcg ccgggatgcc ctcggtgatg cagatcacca gcggcacgcc ggcatcggcc
     3841 gcctccagga cggcgtccgg ggcgaacggc gccggcacga agatgcacga ggcgttggcc
     3901 cccgtctcgc cgaccgcgtc caggacggtg tcgaagaccg gcacccggcc gtccagcgcg
     3961 gactggccgc ccttgcccgg ggtgacaccg gcaaccacgt tggtgccgta ctcgagcatg
     4021 gcgcgggaat ggaactcacc ctcgcgcccg gtgatgccct gcaccaccag ccgcgtgtcg
     4081 gcgttgacca ggatgctcac gatgcgctcc gcgccgcttc gaccgccttg gccgccgcct
     4141 cgtccaggct ggcggccgtg atgaggctgg cctgctccag gatgccggcc gcctcctcgg
     4201 cattggtgcc cacgatgcgc accaccatgg gcacctcacg ctcaaggccg gcccgcgcct
     4261 cgacgatgcc gcgtgccacc tcgtcgccac gagtgatgcc gccgaagatg ttgaccagga
     4321 tggcgtgcac gttggggtcg tccaggatga tgcggaaggc tgccagcacg cggtccgcct
     4381 tggtgccgcc gccgatgtcc aaaaagttcg ccggctcgcc gcccgccagc ttgaccaggt
     4441 ccatggtggt catggccagc cctgcgccgt tgaccatgca gccgatgttg ccgtccagct
     4501 tgatgaagtt gatgcccgcc tcgcgcgccg cgatctcgga gggctcctcc tcgttcaggt
     4561 cgcgcatggc ctccaggtcc gggtgccgga acagcgcgga gtcatcgagc acgatcttgg
     4621 cgtccagggc aagcagccga ccgtcgtcgg tgatcaccag cgggttgatc tcggccaggt
     4681 cggcatccgc ccccatgaag gcctcataca ggccctgcag gaccgatgcg aagtcgcggc
     4741 gcagctcagc ggggatgccc aggaagaagc tcgtccgccg cacgttgtac ggctgcaggc
     4801 cgagcagcgg atggaggggc aggcgcagga tcgcgtcggg attggtgcgc gccgtctcct
     4861 cgatctcgac tccgccctcg gccgaggcga tgaccgtcac cgcccgcgct acccggtcca
     4921 ggatgaggcc gaggtagtac tccttggcga tgtcggccgc cggcgccacc agcacggtcc
     4981 gcaccgtcac gcccttgatg tccaggccga tgatctcgcg tgcgcgctgc tccgcctcct
     5041 ccggcgtggc ggccagcttc acgccgcccg ccttgccgcg cccgccgacc aacacctggg
     5101 ccttgaccac caccggtccg ccgatgcgtt cggccgctgc ccgcgcctcg gctggcgtgg
     5161 tggccacctc accgtccagg agcggcacgc cgtggcgagc caggacctcc ttggaccgat
     5221 actcctggag ctt
//
LOCUS       DATACA010000535         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1441059, whole genome shotgun sequence.
ACCESSION   DATACA010000535 DATACA010000000
VERSION     DATACA010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441059"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VHK28_10825"
     CDS             <1..1254
                     /locus_tag="VHK28_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX2142744.1"
                     /translation="RVAVISDWGSMLADVLSGVGLDQYLDLLVVSAVEGVAKPAPELF
                     RLAYERMGVAAQQAVMIGDSYRADVLGARAAGMDAILLDRDGTASETDVPVARSLTQA
                     VDLATGLAAERERLVAAAETLLVTFLWATSWVLIKVGLNDLDLQPLSFAGLRYALAAL
                     ILAPLGWRAIHAVHRRGEHGLDRALVLRVAVMGVFLYAIAQGAQFAALEVLPAASVSL
                     VLATIPIWVSAVSWLRGGEVASGWQLVGIGVLVAGAALYFGGFDPGTSGLLGLAAAAL
                     CAVASTTGQHLSRDLNRDVQARLGGPIGLTALSMAIGSALLLGVGVALEGWPSLDATG
                     WVIVAWLAVVNTAFAFTLYNRTLRVLTAVESSVIVNLLLVMVAAMAWIFLGETLDLRQ
                     IAGLALATAGVLIVQLAPRLRRRGS"
     gene            complement(1251..2123)
                     /gene="phnE"
                     /locus_tag="VHK28_10830"
     CDS             complement(1251..2123)
                     /gene="phnE"
                     /locus_tag="VHK28_10830"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX2142745.1"
                     /translation="MTLRGATTLDRPAPPSNAKRNLAVLGAAIIVVLSALTVGLEPGE
                     ILDLPGGVGRVFYEMFLRNGVEWDYLPDALRLMLESLQIAWLGTVIGAVISLPLGFFG
                     AKNVSSGWVSNVMRQILNAIRAFPEVVLAIAIMIPIAGIGPVPGALALGVHSIGTLGK
                     LTAEVVEGIDPGPVEAARASGARSLQVQRWGVLPQVLPEIVAFWLYRFEINIRASAIL
                     GVVGAGGIGFVLQQAISFTRFERAGLIIIVIVVATIVVDTISGAVRRRIIEGAEARVT
                     GELIEQPEIADVRP"
     gene            complement(2120..2929)
                     /gene="phnE"
                     /locus_tag="VHK28_10835"
     CDS             complement(2120..2929)
                     /gene="phnE"
                     /locus_tag="VHK28_10835"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX2142746.1"
                     /translation="MTAAVTPSQERPPKPKGSLAVPIGILAFIAITWASASEQFGIGL
                     DIGELIEDLGGGALVLREMANPNWEFVPRTFQAMLETFQMAVLASAIGCGVALPVAFL
                     ASRITAPNSPTYVVDRAVLSVVRAMPDILYALVFVAAVGIGPLAGIAALIFFNIGVVA
                     KLLSETVDGIDAGPIEAGRAAGANRLQTVRWSVLPQVLPNYVAYSLYTFELNIRASTV
                     IGIVGAGGIGRLLQTQYRFFAWENVSVIIIELFVLVFFIELLSIWLRRRLV"
     gene            complement(2926..3714)
                     /gene="phnC"
                     /locus_tag="VHK28_10840"
     CDS             complement(2926..3714)
                     /gene="phnC"
                     /locus_tag="VHK28_10840"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX2142747.1"
                     /translation="MIRFESATVTYPGGVHALRNLTLEIPDGQMMVIVGLSGAGKSTL
                     IRAINGLVPLTSGDVIIDGASVRQAKGKQIRELRSRIGMIFQTFNLVKRTTVLNNVLM
                     GRLHDTGTLRSLLGWYRPEDVEIAMRALERVGIVEKAYVRAANLSGGQQQRVGIARAL
                     AQEPKILLADEPVASLDPPTSHVVMRDLQRINRELGITTIVNLHFLDLAKVYGERIIG
                     LREGELVYDGTGAQADENVFRDIYGRSLTADDVMGEAAATPAAL"
BASE COUNT          582 a         1254 c         1318 g          591 t
ORIGIN      
        1 cgggtcgcgg tgatcagcga ctggggctcg atgctggccg acgtgctgtc cggcgtgggc
       61 ctggaccagt acctcgatct gctggtcgtc tcggcggtcg agggcgtggc caagcctgcg
      121 ccggagctct ttcgcctggc ctacgagcgt atgggggtcg cggcacagca ggcggtcatg
      181 atcggcgaca gctaccgggc cgacgtcctc ggggcgcgcg cggccggaat ggacgccatt
      241 ctgctcgacc gggacggcac ggcctcggag accgatgtgc cggtggcgcg cagcctgacc
      301 caggcggtcg acttggccac gggactggcg gccgagcgcg agcggttggt ggccgcggca
      361 gagaccctgc tggtcacgtt cctgtgggcc acctcctggg tgctgatcaa ggtcggcctc
      421 aacgacctgg acctgcagcc gctctcgttc gccggcctcc gttacgcgct ggcggccctg
      481 atcctggcac cgctcggctg gcgggccatc cacgccgtgc accgccgcgg ggagcacggg
      541 ctggaccgcg cactggtcct gcgcgtggcg gtaatgggcg tcttcctgta cgccatcgcg
      601 cagggcgcgc agttcgcggc gctcgaggtg ctgccggcgg catcggtcag cctggtgctg
      661 gccaccattc ccatctgggt cagcgccgtc tcctggctgc gcggcgggga ggtggcgagc
      721 ggctggcagc tggtggggat cggcgtgctg gtcgccggcg cggcactgta cttcggtggc
      781 tttgacccgg gcacctccgg gctgctgggc ctggctgcgg cggcgctgtg cgccgtggcc
      841 tcgaccaccg gccagcacct ctcccgggac ctgaaccgcg acgtgcaggc ccgcctcggc
      901 gggcccatcg gtctgaccgc gttgtccatg gcgatcggat ctgccctgct gctgggagtt
      961 ggcgtggcgc tcgagggctg gccctcgctg gatgcgacag gctgggtgat cgtggcctgg
     1021 ctggcggtgg tgaacaccgc cttcgccttc accctctaca accgcacgct gcgggtgctg
     1081 accgcggtgg agtccagcgt gatcgtcaac ctgctgctgg tgatggtggc ggccatggcc
     1141 tggatcttcc tgggcgagac gctcgacctt cggcagatcg ccgggctggc ccttgccacg
     1201 gccggggtgc tgatcgtcca gctggccccg cggctgcgac ggcggggaag ctaggggcgc
     1261 acgtccgcga tctccggctg ctcgatcagc tcgccggtga cgcgagcctc ggcgccctcg
     1321 atgatccggc ggcgcaccgc accggagatg gtgtcgacca cgatggtggc aaccacgatc
     1381 acgatgatga tcagtccggc gcgctcgaac cgggtgaacg agatggcctg ctgcaggacg
     1441 aagccgatgc cgccggcacc cacgacgccc aggatcgccg acgcccggat gttgatctcg
     1501 aaccggtaca gccagaaggc cacgatctcc ggaaggacct gcggcagaac gccccagcgc
     1561 tgcacctgca gcgaacgcgc gccgctggcg cgcgccgcct ccaccgggcc cggatcgatg
     1621 ccctccacca cctcggccgt cagcttgccc agggtgccga tggagtggac gccgagcgcc
     1681 agagcgcccg gcaccggccc gatgcccgcg atcgggatca tgatggcgat ggccagcacg
     1741 acttcgggga atgcgcggat ggcgttcagg atctgtcgca tgacgttcga cacccacccg
     1801 ctcgacacgt tcttcgcgcc gaagaacccc agtggcagcg agatgacggc gccgatcacc
     1861 gtgccgagcc aggcgatctg cagcgattcc agcatgaggc gcagcgcatc aggcaggtag
     1921 tcccactcca cgccgttgcg caggaacatc tcgtagaaga cccggccgac accgcccggc
     1981 aagtcgagga tctcgcccgg ctcgaggccg accgtgagcg ccgacaggac gacgatgatt
     2041 gccgccccga gcaccgcgag attgcgcttt gcgttcgatg gtggcgcggg ccggtcaagc
     2101 gtcgtggccc cccggagcgt cacaccagcc tccgacggag ccagatcgac aacagctcga
     2161 tgaagaacac gagcacgaac agctcgatga tgatcaccga gacgttctcc cacgcgaaga
     2221 agcggtactg ggtctgcagc aggcggccga tccctcccgc gccgacgatg ccgatgaccg
     2281 tcgaggcccg gatgttcagc tcgaaggtat agagcgagta ggcgacgtag ttgggcagga
     2341 cctggggcag gacggaccag cggacggtct gaaggcggtt tgcccccgcg gcgcgcccgg
     2401 cctcgatcgg cccggcgtcg atgccgtcaa ccgtctcgga caggagcttg gcgacaacac
     2461 cgatgttgaa gaagatcagg gcggcgatgc cagccagcgg cccgatgccg accgcggcca
     2521 cgaagaccag cgcgtagagg atgtctggca tggctcggac cacgctcagc accgcgcgat
     2581 ccaccacgta ggtaggactg ttcggggcgg tgatgcgcga cgccaggaaa gcgaccggca
     2641 acgccacacc acagccgatg gcggacgcca gcacggccat ctggaaggtc tccagcatgg
     2701 cctggaaggt tcgtggcacg aactcccagt tcgggtttgc catctcccgg agcaccaagg
     2761 ctccaccgcc caggtcctcg atgagctcgc cgatgtccag cccgatgccg aactgctcgc
     2821 tggcggacgc ccaggtgatg gcgatgaatg cgagaatccc gatcgggacg gcgagcgaac
     2881 ccttcggctt cgggggtcgt tcctggctgg gggttacggc ggcggtcaca gcgccgcggg
     2941 cgtcgcggcc gcctcgccca tgacgtcgtc ggcagtcagc gagcggccat agatatcgcg
     3001 gaagacgttc tcatccgcct gggcgccggt gccgtcgtac acgagctcgc cctcgcgcag
     3061 gccaatgatg cgctcgccgt agactttggc caggtccagg aagtgcaggt tgacgatggt
     3121 cgtaatgccc agttcccggt tgatgcgctg caggtcgcgc atgaccacgt ggctggttgg
     3181 cgggtcgagg ctggcgaccg gctcatcggc caggaggatc ttcggctcct gggccagggc
     3241 tcgggcgatg cccacccgct gctgctgacc cccggagagg ttcgcggccc gcacgtatgc
     3301 cttctcgacg atgccgaccc gctcgagggc ccgcatcgcg atctcgacgt cctccgggcg
     3361 gtaccaaccg agcagtgacc gaagcgtccc ggtgtcgtgc aaccgtccca taaggacgtt
     3421 gttcagcacg gtggtgcgct tcaccaggtt gaatgtctgg aagatcatgc cgatccggga
     3481 gcgcagctcg cggatctgct ttcccttggc ctggcggacg ctcgcgccgt cgatgatcac
     3541 gtcaccgctg gtcaacggca caaggccgtt gattgcgcgg atcagcgtgg acttgcccgc
     3601 gccggacagg ccgacgatga ccatcatctg tccgtcggga atctcgagcg tcaggttcct
     3661 gagcgcgtgg acacctccgg gataggtgac cgtcgcgctt tcgaaccgga tcatgcgtga
     3721 aggtcgacga ggggcgagcc tgaga
//
LOCUS       DATACA010000536         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_396244, whole genome shotgun sequence.
ACCESSION   DATACA010000536 DATACA010000000
VERSION     DATACA010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396244"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..761
                     /locus_tag="VHK28_10845"
     CDS             33..761
                     /locus_tag="VHK28_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2142748.1"
                     /translation="MVRLNRPKQLNALNAQVMDELCATLEDLDRDESVRAIVVTGNER
                     AFAAGADIGEMADATPVDMLKGNRIGQWDRIRRIGKPVIAAVNGWALGGGCELAMTLD
                     LIIAGEGAKFGQPEINIGVIPGAGGTQRLTHAIGKSRAMEMILTGEAMDAREAVRHGL
                     VARVVPDELVVEDALALAAKIATKSPIALRLAKEAVNAAYEMSLTDALAHERRLFYLL
                     FASEDQKEGMAAFLEKRTPDFKGR"
     gene            758..1732
                     /locus_tag="VHK28_10850"
     CDS             758..1732
                     /locus_tag="VHK28_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142749.1"
                     /translation="MSLGNPDHPVGGIAVRPPAGVELRPLGRADLPDALALAREQREL
                     PSVTDIELFRDRYDRLINTPDTAAFLALADGAPVGLSALQFRRRLNFATFEGWLSDLY
                     VRPAMRRRGIGRALLEALVAEWRLRGSHRMQAQVPDGAAGGRATLDRAGFEEWMLDFQ
                     LAPVIHPPLDLPEGVSLRPVRADDGEAVTRLIAEFGPHRSPVPDRMEAVLRTFAEHLR
                     AVEAGAATTTIAELEGVAVGVCVMEWREPFWTDAVQAWIHDVVVTEPVRGRGIGRALM
                     ADALGRAVQRGAASVLLESGMQRESAHRLYRSLGFEAVGRTWVLRRDP"
     gene            1698..2321
                     /locus_tag="VHK28_10855"
     CDS             1698..2321
                     /locus_tag="VHK28_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142750.1"
                     /translation="MGAHGCCAGTRERASDYVRRLPTGERLPIGEVINDDLFPFEGEI
                     LVRPLTEPILPEPPRNGEAGGGECHACRGTGEGRIWEDERWWAFMAEEPSGLPAVAVL
                     MPKTHEDLEDLPPDVVGELGGMIQRLARAIGRLESVGRVHVNRWGDGSEHFHIWFLAR
                     PRGMWQLRGAMLAVWDDLLPRVPVEEWLANRRTIAAALAEEGGQALV"
     gene            complement(2325..2636)
                     /locus_tag="VHK28_10860"
     CDS             complement(2325..2636)
                     /locus_tag="VHK28_10860"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX2142751.1"
                     /translation="MSAWLIVLAVAGATATLKAAGPLVLGGRPLPGRVVAVVSLLAPA
                     LLAALIATSTFATGQQLVVDARVLGVGAAVVAALLRAPVLMVVLVAAVVTAMARALGV
                     A"
     gene            complement(2633..>3367)
                     /locus_tag="VHK28_10865"
     CDS             complement(2633..>3367)
                     /locus_tag="VHK28_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX2142752.1"
                     /translation="LRTGRARPRVRRVVGPAAGVSAERNDDIRRGALRAAPFAVAVGL
                     FGIVFGALAAATGPMGALPAVVMSATTFAGSAQFAAVSILAGGGQPLTAVVAAVLLNL
                     RYAPIGVSVAPSLRGAWWRRLLISQLLVDESWAIAADRAGRFKIRVLIGAGVVLWLAW
                     VAGTVLGVVGGETLGDPNRLGLDAAFPALFLALLAPQLRRRRALAAAAIGAGLSLLLV
                     PFTPAGVPIVAASAAALLGLIGARSR"
BASE COUNT          518 a         1170 c         1251 g          428 t
ORIGIN      
        1 tggtcgaacg ccaggacgac cagcgcaccg ccctggtgcg cctcaaccgg ccgaagcagc
       61 tcaacgcgct gaacgcccag gtgatggacg agctgtgtgc caccctcgag gacctggacc
      121 gcgacgaatc ggtccgagcc atcgtggtca ccgggaacga gcgcgccttc gcggccggcg
      181 ccgatatcgg tgagatggcg gacgcgacgc ccgtcgacat gctcaagggc aaccgcatcg
      241 ggcaatggga tcgcatccga cgcatcggca agccggtgat cgccgcggtc aacggttggg
      301 ccctgggtgg tgggtgcgag ctggcaatga ccctggacct gatcatcgcc ggggagggcg
      361 cgaagttcgg gcagccggag atcaacatcg gcgtcattcc gggtgcgggc ggcacgcagc
      421 ggctgaccca cgcgatcggt aagtcccgcg ccatggagat gatcctgacg ggtgaggcga
      481 tggacgcccg cgaggctgta cgccacgggc tggtcgcccg cgtggtcccg gacgagctgg
      541 tggtggagga cgcgctggcc ctggcggcca agatcgccac caagtcgccg atcgcgctgc
      601 gcctggccaa ggaggcagtc aacgccgcgt acgagatgag cctgaccgat gcgctggccc
      661 acgagcggcg cctcttctac ctcctgttcg ccagcgagga ccagaaggag ggcatggcgg
      721 ccttcctgga gaagcggacg cccgacttca agggccgatg agcctgggca accccgacca
      781 tcctgtcggc gggatcgccg tccgtccgcc ggccggggtc gagctgcggc cgctggggcg
      841 cgccgacctg ccggacgcgc tagcactggc ccgggagcaa cgcgagctgc catcggtcac
      901 cgatatcgag ctcttccggg accgatacga ccgcctgatc aacacgccgg acaccgccgc
      961 ctttctggcc ctggccgacg gtgcgccggt tggcctttct gccctgcagt tccgaaggcg
     1021 cttgaacttc gccaccttcg agggctggct atcggacctg tacgtgcggc cagcgatgcg
     1081 tcgacgcggg atcgggcggg cgctgctgga ggcgctcgtc gcggagtggc ggttgcgtgg
     1141 cagccatcgc atgcaggcgc aggtgcccga cggagctgcc ggcggacggg cgacgctgga
     1201 ccgggccggc ttcgaggagt ggatgctcga cttccagctc gccccggtga tccacccgcc
     1261 acttgacctg ccggagggag tcagcctgcg gccggtccgc gctgacgacg gcgaggccgt
     1321 gacccggctc atcgccgagt tcgggccgca ccgctctccc gtgccggatc ggatggaggc
     1381 cgtgctgcgc accttcgccg agcacctccg cgcggtcgag gccggggccg cgacgaccac
     1441 catcgccgag ctcgagggtg tggcggtggg cgtgtgcgtc atggagtggc gcgagccgtt
     1501 ctggaccgac gccgtccagg cctggatcca cgacgtggtg gttaccgagc cggtgcgtgg
     1561 ccgcgggatc gggcgcgccc tcatggcgga tgcgctcggc cgcgcggttc agcgcggagc
     1621 ggcatccgtg ctgctggagt cggggatgca gcgagagtcg gcgcatcgac tgtatcggtc
     1681 tctcggcttc gaggcggttg ggcgcacatg ggtgctgcgc cgggacccgt gagcgagcga
     1741 gcgactacgt tcggcgcctc cccaccgggg agcgcctgcc cataggcgag gtaatcaacg
     1801 acgacctttt tccgttcgag ggtgagatcc tggtcaggcc actgaccgag ccgatcctcc
     1861 ccgagccgcc acgcaacggc gaggcggggg gtggcgagtg ccacgcctgc cgtgggaccg
     1921 gggaaggccg gatctgggag gacgagcgct ggtgggcctt catggctgag gagccctcgg
     1981 ggctgcccgc ggtagccgtg ctcatgccca agacgcacga agacctggag gacctgccgc
     2041 cggatgtggt cggcgagctg ggagggatga tccagcgcct tgcccgcgcg atcgggcgcc
     2101 tggagtcggt gggacgcgtc cacgtcaacc gttggggtga cggcagcgag cacttccaca
     2161 tctggttcct ggcccgaccg cgtgggatgt ggcagctccg tggcgcgatg ctggccgtat
     2221 gggatgacct cctgccccgc gtgcccgtcg aggagtggct ggccaaccgg cggaccatcg
     2281 ccgcggcact agcggaggag ggtgggcagg cgctcgtctg acgactacgc gactccgagc
     2341 gcgcgggcca tggcggtgac caccgctgcc accagcacca ccatcagcac cggggcgcgc
     2401 agcagggcgg ccacgacggc ggcgcccacg cccaggacgc gcgcgtcgac gacgagctgc
     2461 tggcccgtgg cgaaagtgct ggtggcgatc agcgccgcca gcagcgccgg cgccaggagg
     2521 gagacgacgg ccaccacccg gccgggcagc ggacgaccgc ccaggaccag tgggccggcc
     2581 gccttgagcg tggcggtggc tccggccacc gcgagcacga tcagccaggc gctcaccgcg
     2641 aacgcgcccc aatcaggccc agcagcgccg cggcactggc cgccacgatc ggcacaccgg
     2701 cgggtgtgaa cggcaccagc agcagcgaca gcccggcacc gatggcggcg gccgccagcg
     2761 cgcgtcggcg gcgcagctgg ggcgcgagca gggccaggaa cagcgccggg aatgctgcat
     2821 ccagccccag ccggttcgga tcgccaagag tctcgccgcc caccaccccg aggacggtcc
     2881 ctgcgaccca ggccagccac agcacgaccc ctgcgccgat cagcacccgg atcttgaagc
     2941 ggcctgcacg gtcggcggcg atggcccacg actcatcgac caggagctgg ctgatcagca
     3001 gccgccgcca ccaggcgccg cgcagcgatg gcgcgaccga gacgccgatg ggcgcgtagc
     3061 gaagattgag cagcacggcg gcgaccacgg ccgtcagcgg ctggccgcca ccagccagga
     3121 tcgagacggc ggcgaactgc gcggagccgg cgaacgtggt ggccgacatg acgaccgccg
     3181 gcagagcgcc catcggtccg gtggctgctg ccagggcgcc gaagacgatc ccgaacaggc
     3241 cgaccgccac cgcgaacggc gcggcccgca gggcgccgcg ccggatgtcg tcgttccgtt
     3301 ccgcgctcac tccggctgct ggacccacca ctcggcgaac tcgcgggcgc gcccgtccgg
     3361 tgcgaag
//
LOCUS       DATACA010000537         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_237095, whole genome shotgun sequence.
ACCESSION   DATACA010000537 DATACA010000000
VERSION     DATACA010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237095"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VHK28_10870"
     CDS             complement(<1..555)
                     /locus_tag="VHK28_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142753.1"
                     /translation="MVDQAPVPGRSPSPPERTGLRAELRQDKAIYMAALKAARTIGEE
                     TLKALPGGSILLALFDGFEHLAEDAENAELSDRVDRLLDIGRQSQEDIGALTALAALM
                     VSQQALLLERMEETGVAAEPGRLGEAARTAALAAYRGRIAQQFCYVDYRGIAGITREE
                     HAASFEFDHVYVQPRLLPEREVSPL"
     gene            759..1700
                     /locus_tag="VHK28_10875"
     CDS             759..1700
                     /locus_tag="VHK28_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142754.1"
                     /translation="MRGRALLRTLVVALLLAAPTPSSGQDATSGRRVAATLPDLSGLA
                     WLSDDVFVGVHDAKNPKENDRPRVSLLWLPASPEGITWRPVELAWPEPLGPSSDLESA
                     ARIPGTRSLLLVESGEGKAAGEPARRIFLAERVDQDLRIAATAEWPASVKNVEGTAVA
                     RVGNRLIFIYAERAQGQPSTNIRWAPLELQPLGFGAFQEVTFPSPDPPGPDARPVSAI
                     EVDDAGRLYAASAFDPGDDNGPFRSAVWRIGRVEAGDDRGPRVVLDAQPQRLATLDGL
                     KVESLAARQGSGSDTLLYVGTDDENYGGTLRPLPPRP"
BASE COUNT          277 a          711 c          685 g          263 t
ORIGIN      
        1 cagcgggctg acctcccgct cgggcagcag ccgcggctgg acgtagacgt ggtcgaactc
       61 gaacgacgcc gcgtgctcct cgcgggtgat gcccgcgatg ccccggtagt cgacgtagca
      121 gaactgctgc gcgatccggc cgcggtaggc agcgagcgct gcggtcctgg cggcctcgcc
      181 gagccggccc ggctccgccg cgacgccggt ctcctccatc cgctccagga gcagcgcctg
      241 ctgcgagacc atcagcgcgg ccagcgccgt cagcgcgccg atgtcctcct gggactgccg
      301 cccgatgtcg agaagccggt cgacgcgatc gctcagctcg gcgttttccg catcctccgc
      361 caagtgctcg aagccgtcga acagggcgag caggatcgac ccgcccggca gcgccttgag
      421 agtctcctcg ccgatcgtcc tcgcggcctt gagcgcggcc atgtagatgg ccttgtcctg
      481 ccgcagctcg gcgcggaggc cggtgcgctc gggcggagac ggagaccggc cggggaccgg
      541 cgcttgatcg accatgacgc gcaaccacgc cgccgtttga tatcaaaagt tatagacgat
      601 cgaaggccct accgtccatg ccggcctgac cgagccgatc cgcacgcatg cgtgcacgcg
      661 cgcacggcgt ggtacgggtg ccggtcgcgc ccggttcttg catgtattcc cagcatccgc
      721 taaccccaac cgctaggcaa aaattccgag aggaaccgat gagaggccga gccctcctac
      781 gcacgctggt ggtggcgctg ttgctggcgg cgcccacccc gtcctcgggc caggacgcga
      841 cgagcggacg gcgggtggcg gcgacgctgc ccgacctcag cgggctggcc tggctctcgg
      901 acgacgtctt cgtgggcgtc cacgacgcca agaaccccaa ggagaacgac cggccgcgcg
      961 tcagcctgct gtggctgccg gcctcgccgg agggcatcac ctggcggccg gtcgagctgg
     1021 cttggcccga gccgctgggg cccagcagcg acctggagag cgccgcccgc atccccggca
     1081 cgcggtcctt gctcctggtc gagagcggcg agggcaaggc cgcgggcgag ccggcccgcc
     1141 ggatcttcct ggccgagcgc gtcgaccagg atctgaggat cgcggccacc gcggagtggc
     1201 cggcttccgt caagaacgtc gagggcaccg cggtcgcgcg ggtcgggaac cggctgatct
     1261 tcatctacgc cgagcgcgcg cagggccagc cgagcaccaa catccgttgg gcgccgctgg
     1321 agctgcaacc gcttgggttc ggcgcgttcc aggaggtgac gttcccctcc cccgacccgc
     1381 cggggccgga cgcccggccg gtcagcgcga tcgaggtcga cgacgcgggg cggctctacg
     1441 cggcgtcggc gttcgacccc ggcgacgaca acggcccgtt ccgcagcgcc gtctggcgca
     1501 tcggccgggt cgaggcgggc gacgatcgcg ggccccgggt ggtcctggac gcgcagccgc
     1561 agcgtctggc cacgctcgac ggcctgaagg tcgaaagcct cgccgcgcgc caaggctccg
     1621 gcagcgacac cctcctctac gtcggcaccg acgacgagaa ctacggcggc accctacgcc
     1681 cgctgccgcc gcggccctga cggacgaggg ggctccggct caacagcgcc cggatttgcc
     1741 ggcctggtca agccgatcga gcacgcgcgc gccgtaacgc ggggagcggc cgcgcccggt
     1801 ccttgtattt tttcctagca tttgctacac agtagtctct ggggggttgc ccccgaccgc
     1861 ggggcagcgc ccggtgtccc cggcgcgacc ctgggtgcgg gcagcgggcc gacgcgaacg
     1921 gcgcgctcta ccaaac
//
LOCUS       DATACA010000538         4564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2084241, whole genome shotgun sequence.
ACCESSION   DATACA010000538 DATACA010000000
VERSION     DATACA010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084241"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VHK28_10880"
     CDS             <1..396
                     /locus_tag="VHK28_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2142755.1"
                     /translation="EVVLRLTAMLAGGRGQVSLLHVIEVPFERQLDAEDPRAIAFADE
                     VLERGQGFLTDHEVDVRTSIAQARAAGAAIVDDAVEQRADLIIMGLRYKKRFGGGWDA
                     GRTVPYVMRNSTAPVWCLRVETEDLAQTS"
     gene            393..965
                     /locus_tag="VHK28_10885"
     CDS             393..965
                     /locus_tag="VHK28_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX2142756.1"
                     /translation="MRAIIIGCGRVGARTAVELDQRGDHVTVIDTDQRSFSRLPNTFG
                     GVTVRGSGTDEDILRGAGAEMSDLLIALTEGDNRNALAAQLGKHVFGIPRVVAKINDP
                     VRADAFRSLGLETICRTVILADALVKAAAEGAEATDGMVQPPTAEPLRGAPASGTKAA
                     LEAEEATAADGAGDAASGSGTTATPFGGEG"
     gene            971..1630
                     /locus_tag="VHK28_10890"
     CDS             971..1630
                     /locus_tag="VHK28_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX2142757.1"
                     /translation="MYVVVIGGGNVGYYLTKELLAAGHEVVMIEKDPSRARLIADELG
                     SIVIPNDGCEGRYQGEAGMGRADVMCAVTGDDEDNLVACQVAKMRFNVPRAIARVNNP
                     KNEQLFRQLAIDETVSPTRTILGVIEHEIPIHDLLHLTELERGELQIVEAQLDASSPA
                     VGREVRDVTLPEESFVLVIVRNERAIPARPETRLMTGDKVLAITSADRESELRSVLIG
                     E"
     gene            1627..2430
                     /locus_tag="VHK28_10895"
     CDS             1627..2430
                     /locus_tag="VHK28_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142758.1"
                     /translation="MTPASPGRALRLSLLAWGLGDMATGRTTRGAALACVEVLAAVAV
                     AVATVLFVDTTWYLVPFLAGMGFIGLWAVQAVLAYRHAGHSAALEPAGPMDPTPRTGS
                     RAGAVAWLTVPLLVWGTGFWLVAADSATPGAVVDRFVSAWPQVDEEALDASLAVEPAR
                     VGAAAAEALDELDVLCAAGRLPDDCGDARAALLRGVRVRIERPQLEEAVAVAEVVRYE
                     RRPSRFLGIFEGSELVPVPIRSVLTLELGTQAAALGANRWVILNAEAGP"
     gene            2427..3434
                     /locus_tag="VHK28_10900"
     CDS             2427..3434
                     /locus_tag="VHK28_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018187023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2142759.1"
                     /translation="MSRHDMTDTVAAPAAGRPAAKGARFLADVGRAVSVAVVGAEQPL
                     RLLATCLLAEGHALVEDVPGVGKTLLARAFARSLDLSFARVQGTPDLLPTDVTGSSIL
                     DAGEFRFIPGPVFANVLLVDEINRATPRTQSSLLEAMQERQVSAEGQTRPLPDPFLVL
                     ATQNPVELEGTFALPEAQLDRFLLRIAMGYPDEAAERRIASRHREAAEPLDAVPVVAE
                     PAEVDALRRAARTVRVADDVERYLVAVVRATRQHPDVRLGASPRSSVAFYRAAQAWAL
                     LDGRDFVLPDDARAIAHQVLAHRLLLDVDRELRGASVGSVLDEVLEATPVPMVDDPEG
                     R"
     gene            3460..>4564
                     /locus_tag="VHK28_10905"
     CDS             3460..>4564
                     /locus_tag="VHK28_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2142760.1"
                     /translation="MSGWVALAILIAATGAVAGAPGLLLVGAVTLAYGSVTRLWTRYG
                     MRRVTYGRRLSAERAVAGDSLILDVTIWNRKPLPLPWVAAEDLVSEQLPVRERPTMDR
                     DNEIGGRRILHNAWSLAWYERVIRHFHLDDLRRGVYDLGPVRIRVRDILGRDAVEDEQ
                     ELSDTLVVSPRSLPVRGAGAEAAPIGERRARRSLHADPALYGGVRPFQPGDSLRRVHW
                     RASARVGTPVSRRYEPARGRQVVLVLDVQTIEGPHWDMTWDEPAFEGLCITAASLARQ
                     LLHDGASVGIAAASFSGTTQRFAWLAPQAAPAQLTRIGGVLARIGPVSSAPFGLLLTW
                     LTRRVPSGVTLLVLSARDPAANLPALRRLARSGY"
BASE COUNT          590 a         1642 c         1706 g          626 t
ORIGIN      
        1 gaggtcgtgc tgcgcctgac cgccatgctg gccggcggtc gcgggcaggt ctcactgctg
       61 cacgtcatcg aggtcccgtt cgagcggcag ctggacgcgg aggacccgcg cgccatcgcc
      121 ttcgcggacg aggtgctgga gcgcggccag ggcttcctga ccgaccacga ggtggacgtg
      181 cgcaccagca tcgcccaggc gcgggccgcc ggggcggcga tcgtggacga cgccgtggag
      241 cagcgcgccg acctgatcat catgggacta cgctataaga agcgcttcgg tggagggtgg
      301 gacgccgggc ggaccgtacc ctacgtcatg cgcaactcca ccgcgccggt ctggtgcctg
      361 cgcgtcgaga ctgaggacct ggcacagacg tcatgagagc aatcatcatc ggctgcgggc
      421 gggtcggcgc gcgcaccgcg gtcgagctcg accagcgcgg cgaccacgtg acggtcatcg
      481 acaccgacca gcgctcgttc agccgcctgc cgaacacctt cggcggcgtc accgtgcggg
      541 gcagcggcac ggacgaggac atcctgcgcg gcgccggcgc cgagatgagc gacctgctga
      601 tcgcgctgac cgagggcgac aaccgcaacg ccctggccgc ccagctgggg aagcacgtct
      661 tcggcatccc gcgcgtggtg gccaagatca acgatccggt gcgggcggac gccttccgct
      721 cgctcgggct ggagaccatc tgccgcaccg tcatcctggc ggacgcgctg gtgaaggcgg
      781 cggccgaggg ggcggaagcc accgacggca tggtccagcc gcccaccgcc gagccgctgc
      841 gcggcgcgcc ggccagcggc accaaggcgg ccctggaggc agaggaggcg accgcggccg
      901 acggcgcagg cgacgcggcg agcggctccg gcaccacggc gacgccgttt ggcggggagg
      961 gctgaacgac atgtacgtcg tggtcatcgg tggaggaaac gtcggctact acctgaccaa
     1021 ggagctgctg gccgccggtc acgaggtggt catgatcgag aaggacccgt cgcgggccag
     1081 gctgatcgcg gacgagctgg gcagcatcgt gatccccaac gacggctgcg agggccgcta
     1141 ccagggcgag gccggcatgg gtcgcgcgga cgtgatgtgc gccgtcaccg gcgacgacga
     1201 ggacaacctg gtcgcctgcc aggtggccaa gatgcgcttc aacgtgccgc gcgccatcgc
     1261 ccgcgtgaac aacccgaaga acgagcagct cttccggcag ctggccatcg acgagacggt
     1321 gagccccacc cgcaccatcc tgggcgtgat cgagcacgag atcccgatcc acgacctgct
     1381 gcacctgacc gagctggagc gcggagagct gcagatcgtg gaggcccagc tggatgccag
     1441 ttcgccggcc gttggccgtg aggttcgcga cgtcacgctg cccgaggaga gcttcgtgct
     1501 ggtcatcgtg cgcaatgagc gggccatccc ggcgcgtccc gagacgcgcc tgatgaccgg
     1561 cgacaaggtg ctggccatca cctccgccga tcgcgagtcg gagctgcgca gcgtcctgat
     1621 cggcgagtga cgcccgcctc cccgggccgg gcgctgcgcc tctcgctgct ggcctggggg
     1681 ctgggcgaca tggccaccgg gcgcaccacc cgcggcgctg cgctggcatg cgtcgaggtg
     1741 cttgccgccg tggccgtggc ggtggccacc gtgctgttcg tggacaccac ctggtacctg
     1801 gtccccttcc tggccggcat gggcttcatc ggtctatggg ccgtgcaggc cgtgctcgcc
     1861 taccgccacg cgggccattc ggctgcgctt gagcccgcag gaccgatgga tcccactccc
     1921 cgcaccggct cgcgcgccgg cgccgtggcc tggttgaccg tcccgctgct ggtgtggggc
     1981 accggcttct ggctggtggc ggccgattca gccacgcccg gggccgtggt ggacagattc
     2041 gtgtccgcct ggccacaggt cgacgaggag gcgctggacg cctcgctggc ggtggagccg
     2101 gcccgggtcg gcgcggccgc ggcggaggca ctcgacgagc tggatgtgct gtgcgccgcc
     2161 gggcgcctgc cggacgactg cggggacgcg cgggccgcgc tgctgcgcgg cgtccgggtc
     2221 cgcatcgaac gcccgcagct ggaggaggcc gttgcggtgg ccgaggtggt gcgctacgag
     2281 cgacgcccct cgcgcttcct gggcatcttc gaggggagcg agctggtacc ggtccccatc
     2341 cgctccgtcc tgaccctgga gctcggcacc caggccgccg ccctgggcgc gaatcgatgg
     2401 gtcatcctga acgcggaggc cggtccatga gtaggcatga catgaccgat acggtggcgg
     2461 cacccgccgc gggccgcccc gcggccaagg gtgcgcgctt cctggcggac gtgggacggg
     2521 cggtctccgt ggccgtggtg ggtgccgagc agccgctgcg actgctggcc acctgcctgc
     2581 tggccgaggg ccacgcgctc gtggaggacg tgcccggcgt gggcaagacg ctgctggcgc
     2641 gcgccttcgc gcggtcactg gacctcagct tcgcccgcgt gcagggcacg ccggacctgc
     2701 tgccgaccga cgtgaccggc tcgagcatcc tggacgccgg cgagttccgc ttcatccccg
     2761 ggccggtctt cgccaacgtg ctgctcgtcg acgagatcaa ccgcgccacg ccgcgcaccc
     2821 agtcgtcgct gctggaggcc atgcaggagc gccaggtgag cgccgaaggc cagactcgcc
     2881 cccttcccga cccgttcctg gtgctggcca cccagaaccc ggtggagctg gaggggacat
     2941 tcgcgctgcc cgaggcgcag ctcgaccgct tcctgctgcg catcgccatg ggttacccgg
     3001 acgaggccgc cgagcggcgc atcgcctccc gccaccgcga ggcggccgag ccgctggacg
     3061 ccgtgccggt ggtcgcggag ccggccgagg tggatgccct gcgccgggcg gcgcgcacgg
     3121 tgcgggtggc cgacgacgtg gagcgctacc tggtggccgt ggtgcgcgcc acgcggcagc
     3181 accccgacgt gcggctgggg gcctcgcccc gcagcagcgt ggccttctac cgcgcggcgc
     3241 aggcctgggc gctgctggac ggccgcgact tcgtgctgcc ggacgacgcg cgagccatcg
     3301 cgcaccaggt gctggcccac cggctcctgc tggacgtcga ccgcgagctg cgcggcgcat
     3361 cggtcggcag cgtgctggac gaggtgctgg aggccacgcc ggtgcccatg gtggacgacc
     3421 cagagggccg ctgacgcccg gcgtggcggg caccgcatta tgagtggctg ggtcgccctg
     3481 gccatcctga tcgccgccac cggcgcggtg gccggcgccc cggggctgct gctggtgggc
     3541 gccgtgaccc tggcctacgg cagcgtgacc cggctctgga cgcggtatgg catgcggcgc
     3601 gtgacctacg gccggcggct gtcggccgag cgggcggtgg ccggcgactc gctgatcctg
     3661 gacgtcacca tctggaaccg caagccgctg ccgctgccct gggtcgcggc cgaggacctg
     3721 gtcagcgagc agctcccggt gcgcgagcgg ccgaccatgg accgcgacaa cgagatcggg
     3781 ggccggcgca tcctgcacaa cgcctggtcc ctggcctggt acgagcgcgt cattcgccac
     3841 ttccacctgg acgacctgcg gcgcggcgtg tatgacctgg gaccggttcg catccgtgtc
     3901 cgcgacatcc tggggcgcga cgcggtggag gacgagcagg agctttccga caccctggtc
     3961 gtctcgccgc gcagcctgcc ggtgcgcggc gccggggcgg aggccgctcc catcggcgag
     4021 cggcgggcac gccgttcgct ccacgcggac ccggcgctct acggcggagt tcggcccttc
     4081 cagccgggcg actcgctgcg gcgcgtccac tggcgggcca gcgcccgcgt cggaacgccg
     4141 gtcagccgcc gctacgagcc ggcgcgcggg cggcaggtgg tgctggtgct ggacgttcag
     4201 accattgagg gtccgcactg ggacatgacc tgggacgagc cagccttcga gggcctgtgc
     4261 atcaccgccg cgtcgctcgc ccgccagctg ctgcacgacg gcgcctccgt cgggatcgcc
     4321 gccgccagct tcagcgggac gacacagcgc ttcgcctggc ttgcgccgca ggcggctccc
     4381 gcccagctga cgcgcatcgg cggcgtgctg gcccgcatcg gtccggtgag ctcggcgccc
     4441 ttcgggctgc tgctcacctg gctgacccgg cgcgtcccct cgggcgtcac cctgctggtg
     4501 ctgtccgcgc gcgaccccgc cgccaacctg ccggccctgc gacgcctggc acgctcgggc
     4561 tacg
//
LOCUS       DATACA010000539         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2003072, whole genome shotgun sequence.
ACCESSION   DATACA010000539 DATACA010000000
VERSION     DATACA010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003072"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>1795
                     /locus_tag="VHK28_10910"
     CDS             168..>1795
                     /locus_tag="VHK28_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX2142761.1"
                     /translation="MASDRFRGLTRLFSARQVVEQRGTIPSGYAVARRAAERLHARLR
                     ELFAERHSVTTFGPYSPGQAVAMKRLGIEAIYLGGWATSAKGSADEDPGPDLASYPLS
                     QVPDEASGIVRALLTADRNQRFARSRMTDEERDATPEVDFTPLIIADADTGHGGDAHV
                     RNLIRRFVEAGVPGYHIEDQKPGAKKCGHQGGKVLVPVDEQIKRLNAARFQLDVMEVP
                     GVVVARTDAEAANLLDGGADERDQPFILGVTNPDVPSYKAGFLAVLRRLHKAGLADVN
                     GHLLYDLPAEDEAAADAWLDGAGVAREVECIAREMPGAHPEAAVDAAADAFLAAWEPA
                     AGLKTLGEAVADVMAFRTEEGVGLEVSVEEWRTFAASAPWSVARSAARRMGLRVTWEP
                     DRAMSPEGYHQVRGGIGYAIARSLAVAPFADVLWMETKTADLDEAREFAEAVHAAYPD
                     KLLAYNLSPSFNWDTTGMSEEQMRSFPDELGRLGYVFNFITYGGHQVDGLAAEEFATA
                     LREDGMLALARLQRKLRLLESPYRTPQTLVGGPRA"
BASE COUNT          265 a          637 c          651 g          242 t
ORIGIN      
        1 gctgagcgtg gcggagctcg acgagtgccg ctgccctgag ctctgcaacc gcgaccacgc
       61 caacgagtag ggcggccgtg gcagtcactg cgcaacgccc ggcgaccgag gccacgccgc
      121 gcgccgccga cgccttcacc accgaggtgg aggacaccac ccgctggatg gccagcgacc
      181 gctttcgcgg cctgacgcgc ctcttctccg cgcgccaggt cgtcgagcag cgcgggacga
      241 ttccgtccgg ctacgccgtg gcgcgccgtg ccgccgagcg actgcacgcc cgcctgcgcg
      301 agctgttcgc cgagcggcat tcggtgacca cctttggtcc atactcgccg ggccaggccg
      361 tggccatgaa gcgcctgggg atcgaggcca tctacctcgg cggctgggcg acatcggcca
      421 agggctcggc tgacgaggac ccggggccgg atctcgccag ctatcccctc tcccaggttc
      481 ccgacgaggc gtccggcatc gtccgcgcgt tgctgacggc cgaccgcaac cagcgcttcg
      541 cccgctcgcg catgactgat gaagagcgcg acgcgacgcc ggaggtggac ttcacgccgc
      601 tgatcattgc cgatgccgat accggccacg ggggtgacgc ccacgtccgc aacctgatcc
      661 ggcgcttcgt ggaggcgggg gtgcccgggt accacatcga ggaccagaag cccggcgcca
      721 agaagtgcgg gcaccagggc ggaaaggtgc tcgtcccggt ggacgagcag atcaagcggc
      781 tcaacgccgc gcgcttccag ctggacgtca tggaggtccc cggcgtggtg gtggcacgca
      841 ccgatgccga ggccgccaac ctgctcgacg gcggcgccga cgagcgggac cagccattca
      901 tcctgggcgt caccaacccg gacgttccct cgtacaaggc gggattcctg gccgtcctgc
      961 gccggctgca caaagccggc ctggccgacg tcaacggcca cctgctctac gacctgccgg
     1021 ccgaggacga ggcggcggcc gatgcctggc tcgacggcgc tggcgtcgcc cgcgaggtgg
     1081 aatgcatcgc gcgggagatg ccgggagcgc acccggaggc tgccgtcgac gccgcagccg
     1141 atgcgttcct ggccgcctgg gagccggccg cagggctcaa gacgctcggc gaggcggtcg
     1201 ccgacgtgat ggccttccgg acggaggagg gggtgggcct ggaagtgtcg gtcgaggagt
     1261 ggcgcacctt cgcggccagc gccccgtggt cggtcgcccg ctcggcggca cggcgcatgg
     1321 gcctgcgggt gacctgggag ccggatcggg ccatgtcgcc ggagggctac caccaggtgc
     1381 gcggtggcat cgggtatgcg atcgcccgct cccttgccgt ggcgcccttc gccgacgtgc
     1441 tgtggatgga gaccaagacc gccgacctgg acgaggcgcg cgagttcgcc gaggcggtcc
     1501 acgccgccta cccggacaag ctgctggcct acaacctgtc gccatcgttc aactgggaca
     1561 ccaccggtat gagcgaggag cagatgcgct ccttcccgga cgagctgggc aggctggggt
     1621 acgtgttcaa cttcatcacc tacggcggcc accaggtgga cggcctggcg gccgaggagt
     1681 tcgccaccgc gctgcgcgag gacgggatgc tggccctggc ccggctgcag cggaagctca
     1741 ggctgctcga gtcgccgtac cggacgccgc agaccctcgt cggcggaccg cgggc
//
LOCUS       DATACA010000540         6223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1360984, whole genome shotgun sequence.
ACCESSION   DATACA010000540 DATACA010000000
VERSION     DATACA010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360984"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VHK28_10915"
     CDS             complement(<1..607)
                     /locus_tag="VHK28_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2142762.1"
                     /translation="MRDTDRLIPFTSESVTEGHPDKLCDAVADAVLDDVIARDPNARV
                     AVEVAATTGLVLVLGEMTTEAYCDVQAVVRDTVREIGYNDSRLGFDWETCGTLTAIKE
                     QSADIALGVDHALEARQGLDPDELGAGDQGMMFGYAVDETPELMPMPIQLAHQITRRL
                     SEVRKRRELPFLRPDGKSQVTIEYDADGRPARVATVVVSSQH"
     gene            complement(640..1914)
                     /gene="ahcY"
                     /locus_tag="VHK28_10920"
     CDS             complement(640..1914)
                     /gene="ahcY"
                     /locus_tag="VHK28_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX2142763.1"
                     /translation="MATRPEVRGDIADIGLADAGALKIEWADRQMPVLAGIRERFERE
                     RPLEGWRISACLHVTAETANLMRTLKAGGADAVLCSSNPLSTQDDVAASLVAHHGIPT
                     YAIKGEDRDSYYSHIGAALDHQPQVTMDDGADLVNELHTRRTQLLNGVVGGTEETTTG
                     VIRLRAMAKAGKLAYPIVAVNEALTKHLFDNRYGTGQSSIDGILRATNILFAGKTVVV
                     GGYGWVGRGIASRAHGMGANVVVVEVDAVRALEAAMDGYRVMNAADAARAGDVFISAT
                     GNLNVWGGDHFPQMRDGAILANAGHFNDEFELGALEQQAASSREVRPFTREYLMDDGR
                     RIYVLADGRLVNLGAAEGHPAMVMDTSFANQALGLEWLRSNLEGLEPRVYGIPDDIDA
                     EVARLKLESMGIRIDPMTAAQAAYADSWESGT"
     gene            complement(1893..2753)
                     /locus_tag="VHK28_10925"
     CDS             complement(1893..2753)
                     /locus_tag="VHK28_10925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX2142764.1"
                     /translation="MAVAPRTELYARAVSDREEIAAFLRTDRIYAAYAFGDLDGSNRQ
                     RCTWGIAYDDLGTPIALAMHTEGLIPQPLFLMGDPIGCHEVLAGVIRPRDAYLMGNDD
                     IDAAVSDLYELDPPQYLLRMAVTADTFRPFAGPAERLVPADIDELNRLYQAGFRGGFP
                     SSVLADGVYYGVRVRGRLVSAAGTHVINQREGIAVVGNVLTHIDYRGHDFAKMVTSAV
                     TSELLARVPDVTLNVHADNEPAVAAYARLGYGERDRLTERLARRRSGGWGLMRPIREA
                     IRLPWQRDPR"
     gene            complement(2775..3302)
                     /locus_tag="VHK28_10930"
     CDS             complement(2775..3302)
                     /locus_tag="VHK28_10930"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX2142765.1"
                     /translation="MTVSVDDLRAKIREVPDFPKPGILFYDITTLLKEPGSFAGVIDR
                     MAEVVKDEKVDLVVGMESRGFIFAAPLAYKLGAGFVPVRKLGKLPAETIEVEYDLEYG
                     TATLEVHRDAIQAGQRVLIVDDLLATGGTVLGTIELVQRLGGEIAGLSFMVELTALRG
                     REKLSDFAIHTLLTY"
     gene            complement(3382..>6223)
                     /locus_tag="VHK28_10935"
     CDS             complement(3382..>6223)
                     /locus_tag="VHK28_10935"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX2142766.1"
                     /translation="EPAGARDTLRVGDRSYDFFRLDAAGATNLATLPYTVKVLLENAL
                     RQSASGDGLVSAEDVRALASWDPKNPGEYELPFLPARVILQDFTGVPCVVDLAAMRDA
                     VAEMGGDPRRINPLVPADLVIDHSVQVDQFGTAGAFLINVEREYERNGERYALLRWAQ
                     QAFDNFRVVPPGTGIVHQVNLEFLADVVTERDGIVFPDTLVGTDSHTTMINGLGVVGW
                     GVGGIEAEAALLGQPLYQPLPTVVGFRLDGELRSGATATDLVLTVTEMLRAHGVVGKF
                     VEFHGPGLSRLPLADRATISNMSPEFGATATLFPVDDETLRYLRMTGRPEEVVQRVEA
                     YAREQGMFRTDASPEPVFNESLSLDLAEVEPSVAGPKRPQDRVPLAMVRENFRGIFGE
                     GLHSHSDGSSHDHRDTAAPRETVVEEASEDSFPASDPPSYTSRTDDGGPQPGQPGEVT
                     PIHTNHDGSHYKAVEVSSNGESFKLRSGSVVIAAITSCTNTSNPSVMVAAGLLARNAL
                     ARGLRTPPWVKTSLAPGSRAVTDYLGNAGLMEPLEQLGFGLVGYGCTTCIGNSGPLAE
                     PIAEAIETNDLVGVAVLSGNRNFEGRIHPLARASYLASPPLVVAFGLAGRIDLDLTTE
                     PLGTDGDGKPVYLSEIWPSAEEVASVVTEQVRGEIFTRSYASVFEGDEQWKTLPVPPG
                     DRYAWDPESTYVALPPFFSGLSPEPEPPQDIVGARVLAALGDSVTTDHISPAGAIART
                     SPAGQWLMERGVEPRDFNSYGSRRGHHEVMMRGTFANIRLRNLLTPDAEGNVTEHLPS
                     GERMSIFDAAMRYADEGTPLVVLAGKEYGSGSSRDWAAKGPYLQGVRAVIAESYERIH
                     RSNLVGMGILPLQYLPGESASSLGLTGRETFSIEGVAGLRPHQEVGVTARGEDGREIR
                     FRAVARLDGEIDVTYYRNGGVLQTVLRRLASS"
BASE COUNT          893 a         2207 c         2142 g          981 t
ORIGIN      
        1 cgtgctggct gctgaccacc acggtcgcca cccgtgccgg acgcccgtcc gcgtcatact
       61 cgatagtcac ctggctcttg ccgtccgggc gcaggaaagg cagctcgcgg cgcttgcgca
      121 cctcggacag gcgccgcgtg atctggtgcg ccagctggat gggcatgggc atcagttcgg
      181 gcgtctcgtc gaccgcgtag ccgaacatca ttccctggtc accggccccc agctcgtcgg
      241 ggtcgagccc ctgcctggcc tccagggcgt ggtccacgcc cagcgcgatg tccgccgact
      301 gctccttgat cgcggtcagc gttccgcacg tctcccagtc gaagcccagg cggctgtcgt
      361 tatagccgat ctcgcgaacg gtgtcgcgca ccacggcctg gacgtcgcag taggcctccg
      421 tggtcatctc gcccagcacc agcaccaggc cggtggtggc cgccacctcg accgccaccc
      481 gcgcgttcgg atcgcgggcg atcacgtcgt ccagcaccgc atcggccacc gcgtcacaca
      541 gcttgtcggg atgcccctcg gtgaccgatt cggaggtgaa cgggatcagg cggtctgtgt
      601 ctcgcatggc cgagagtgta ccgtcccgcc ggtcgcggcc tacgtgccgg actcccacga
      661 atcggcgtag gcggcctggg cggcggtcat gggatcgata cggatgccca tgctctccag
      721 cttgagcctc gcgacctcgg cgtcgatgtc gtccgggatg ccgtagaccc gcggctcgag
      781 cccttcgagg ttcgaccgca gccactccag ccccagcgcc tggttcgcga agctcgtgtc
      841 catgaccatg gctggatgac cctcggcggc tcccaggtta accagccggc catcggccag
      901 gacgtagatg cgccggccat cgtccatcag gtactcgcgg gtgaagggcc gcacctcccg
      961 cgaggaggcc gcctgctgct ccagcgcgcc gagctcgaac tcgtcgttga aatggccggc
     1021 gttggccagg atggccccgt cgcgcatctg cgggaagtgg tcgccgcccc acacgttcag
     1081 gttgccggtg gcactgatga acacgtcacc ggcacgcgca gcatcggccg cgttcatcac
     1141 gcggtagccg tccatggccg cctccagggc gcgaaccgcg tccacctcga ccaccaccac
     1201 gttggcgccc atgccgtgcg cccgggacgc gatcccgcga ccgacccagc cgtagccgcc
     1261 gaccaccacg gtcttgccgg cgaacaggat gttggtggcg cgcaggatgc cgtcgatcga
     1321 gctctggccg gtcccgtagc ggttgtcgaa caggtgcttg gtcagcgcct cgttgacggc
     1381 cacgatcggg taggccagct tgccggcctt ggccatggcc cgcagccgga tgacgccggt
     1441 ggtcgtctcc tctgtgccgc ccaccacgcc gttcagcagt tgggtgcgcc gcgtgtgcag
     1501 ctcgttcacc aggtccgcgc cgtcatccat ggtgacctgc ggctggtgat ccagtgcggc
     1561 accgatgtgc gagtagtagg aatcgcggtc ctcgcccttg atggcatagg tcgggatgcc
     1621 gtggtgcgcc accagggagg cggccacgtc gtcctgggtg gagagcgggt tggacgagca
     1681 gagcacggca tcggccccgc ccgccttgag ggtccgcatc aggttggccg tctcggcggt
     1741 cacgtgcagg caggcgctga ttcgccagcc ttccagcggc cgctcgcgct cgaagcgttc
     1801 ccggatgccg gccagcaccg gcatctggcg gtctgcccat tcgatcttca gggcgccggc
     1861 atcggccagg ccgatgtccg cgatgtcgcc tcttacctcg ggtcgcgttg ccacggcagt
     1921 cgaatcgcct ctcgtatcgg tctcatcagc ccccagccgc cggaacgacg tcgcgccagg
     1981 cgctccgtca gccggtcgcg ctcaccgtag cccagccggg cgtaggccgc cacggcgggc
     2041 tcgttgtcgg cgtgcacgtt gagcgtcacg tccggcacgc gagccagcag ctccgaggtg
     2101 accgcgctgg tcaccatctt tgcgaagtcg tgtccgcggt agtcgatgtg ggtgagcacg
     2161 ttgccgacca ccgcgatccc ctcccgctgg ttgatgacgt gcgtcccggc cgcgctcacc
     2221 agccggccgc ggacccggac gccgtagtac accccgtcgg ccagcacgct ggacgggaag
     2281 ccgccgcgga agccggcctg gtagaggcgg ttcagctcgt cgatgtccgc cggcaccagc
     2341 cgctcggccg gaccggcgaa gggacggaag gtgtcggcgg tgaccgccat gcgcagcagg
     2401 tactgcggcg ggtcgagctc gtagagatcg ctgaccgcgg catcgatgtc gtcgttgccc
     2461 atcaggtagg cgtcccgcgg gcggatgacg ccggccagga cctcgtggca gccaatcggg
     2521 tcgcccatca ggaacagcgg ctgcgggatc agcccctcgg tgtgcatggc cagcgcgatc
     2581 ggtgtcccca gatcgtcgta ggcgatgccc cacgtgcacc gctggcgatt cgagccgtcc
     2641 aggtcgccga aggcgtaggc ggcgtagatg cggtcggtgc gaaggaaggc cgcgatctcc
     2701 tcgcggtcgg agaccgcccg agcgtaaagc tcggtgcggg gcgccactgc catcgggccc
     2761 gctcctcctg tcgctcagta ggtcaggagg gtatggatgg cgaagtcgga cagcttctcg
     2821 cgtccccgca gggcggtcag ctcgaccatg aagctgagcc ccgcaatctc gccgccaagt
     2881 cgctgcacca gctcgatggt gcccagcacc gtgccgccgg tggccagcag gtcgtccacg
     2941 atgagcacgc gctgcccggc ctggatcgcg tcgcggtgca cctccagggt ggcggtgccg
     3001 tactccaggt cgtactcgac ttcgatcgtc tcggccggca gcttcccgag cttgcgcacc
     3061 gggacgaagc cggcgcccag cttgtacgcc agcggcgcgg cgaagatgaa cccgcgcgac
     3121 tccatgccga ccaccaggtc gaccttctcg tccttgacca cctccgccat gcgatcgatg
     3181 acgccggcga acgatcccgg ctccttgagc agggtggtga tgtcgtagaa caggatcccc
     3241 ggcttcggga agtcgggaac ctcgcggatc ttggcgcgca gatcgtcgac ggagactgtc
     3301 acggccggtg ggcgctccag gtgcgttcga agggcctacg gagtctagac cgatgctgcc
     3361 gccgcgggca acggtccagc ctcagctgct cgccagcctg cgcaggacgg tctgcagcac
     3421 gccgccgttg cggtagtagg tcacgtcgat ctccccgtcc aggcgcgcca cggcccggaa
     3481 tcggatctcg cgtccgtcct cgccgcgcgc cgtgaccccc acctcctggt gcgggcgcag
     3541 cccggccacg ccctcgatgg agaaggtctc acgaccggtc agccccaggc tggacgcgct
     3601 ctctcccggc aggtactgca gcggcaggat gcccatcccc accaggttgg accgatggat
     3661 gcgttcgtac gactcggcga tgaccgcccg cacgccctgg aggtagggcc ccttggcggc
     3721 ccagtcccgc gagctgcccg agccgtactc cttgccggcc agcaccacca gcggcgtgcc
     3781 ctcgtcggcg tagcgcatgg cggcgtcgaa gatgctcatc cgttcccccg acggcaggtg
     3841 ctcggtcacg ttcccctcgg cgtccggcgt gagaagattc cgcagccgga tgttggcgaa
     3901 ggtgccgcgc atcatcactt cgtgatggcc gcgccgcgag ccgtacgagt tgaagtcgcg
     3961 cggctcgacc ccgcgctcca tgagccactg cccggccggc gaggtgcgtg cgattgcccc
     4021 ggccggcgag atgtgatcag tggtcacgct gtcgcccagg gcggccagca cgcgcgcccc
     4081 cacgatgtcc tgcggcggct cgggctccgg cgacagcccg ctgaagaacg gcggcagcgc
     4141 cacgtaagtg ctctccgggt cccaggcata gcggtccccc ggcggcaccg gcagcgtctt
     4201 ccactgctca tcgccctcga agaccgatgc gtagctgcgc gtgaagatct cgccacgcac
     4261 ctgctcggtc accaccgacg cgacctcctc cgcggagggc cagatctcgg agaggtagac
     4321 cggcttgccg tcgccatcgg tccccagcgg ctcggtggtc aggtccaggt cgatgcgacc
     4381 ggccagcccg aaggcaacca ccagtggcgg cgaggccaga tagctggccc gcgccagcgg
     4441 atggatgcgg ccctcgaagt tgcggtttcc ggacagcacg gccacgccca ccaggtcgtt
     4501 ggtctcgatg gcctccgcga tcggctccgc cagcggtccg gagttgccga tgcaggtcgt
     4561 gcagccgtaa ccgacaagcc cgaagcccag ctgctcgagg ggctccatca gcccagcatt
     4621 gccgaggtag tcggtgacgg cccgcgaccc cggcgccagc gaggtcttga cccacggcgg
     4681 ggtgcgcagc ccgcgcgcca gcgcgttgcg cgccagcagg ccggcagcca ccatcacgct
     4741 cgggttgctg gtgttggtgc agctggtgat cgccgcgatc accaccgacc cggagcgcag
     4801 cttgaaggac tccccgtttg aggagacttc caccgccttg tagtggctgc cgtcgtggtt
     4861 ggtgtggatt ggcgtcacct cgcccggctg accgggctgc gggccgccgt catcggtccg
     4921 gctggtgtac gacggcggat cgctggccgg gaacgagtcc tcggatgcct cctccaccac
     4981 cgtctcgcgc ggcgcggccg tgtccctgtg gtcgtgcgag ctgccgtccg aatggctgtg
     5041 gaggccctcg ccgaagatgc cgcggaagtt ctcgcggacc atggccagcg ggacgcggtc
     5101 ctggggtcgc ttcggtccgg cgaccgatgg ctcgacctcg gccaggtcca ggctgagcga
     5161 ctcgttgaag acaggctccg gggaggcatc ggtccggaac atgccctgct ccctggcgta
     5221 ggcctccact cgctggacga cctcttccgg gcggccggtc atgcgcaggt agcgcagcgt
     5281 ctcgtcgtcc accgggaaga gggtggcggt cgcgccgaac tccggcgaca tgttgctgat
     5341 ggtggcgcgg tcggccagcg gcaggcgcga caggcccggc ccgtggaact ccacgaactt
     5401 tcccaccacg ccgtgggcac gcagcatctc ggtcaccgtc agcaccaggt cggtggcggt
     5461 ggcgccggaa cgcagctcgc cgtccaggcg gaagcccacc acggtgggca ggggctggta
     5521 cagcggctgg ccgagcagcg cggcctcggc ctcgataccg cccacgcccc agcccaccac
     5581 gccgaggccg ttgatcatgg tcgtgtgcga gtccgtcccg accagcgtgt ccgggaagac
     5641 gatgccgtcc cgctcggtga ccacgtcggc caggaactcc aggttgacct ggtgcacgat
     5701 ccccgtgccg ggcggcacca cccggaagtt gtcgaaggcc tgctgcgccc agcgcaggag
     5761 cgcgtagcgc tcaccgttgc gctcgtactc gcgctcgacg ttgatcagga aggcgccggc
     5821 ggttccgaac tggtcgacct ggacggagtg gtcgatgacc aggtcggccg ggaccagcgg
     5881 gttgatgcgc ctcgggtcgc cgcccatctc ggccaccgcg tcgcgcatgg cagccaggtc
     5941 cacgacacat gggacgccgg tgaagtcctg gaggatgacc cgggccggga ggaacggcag
     6001 ctcgtactcg ccggggttct tcggatccca cgaggccagg gcgcgcacgt cctccgcgct
     6061 gaccaggcca tcgccggatg ctgactggcg cagggcgttc tccagcagga ccttgacggt
     6121 gtagggcagc gtcgccaggt tggtggcgcc ggcggcgtcc agccggaaga agtcgtagga
     6181 acggtcgccg acgcgcaggg tgtctcgggc accggctggc tcg
//
LOCUS       DATACA010000541         7394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2887772, whole genome shotgun sequence.
ACCESSION   DATACA010000541 DATACA010000000
VERSION     DATACA010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2887772"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..962)
                     /locus_tag="VHK28_10940"
     CDS             complement(171..962)
                     /locus_tag="VHK28_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX2142767.1"
                     /translation="MRPGYVWAVFGKEWRDLLSNRLLLGAVVLPALIFAAIPTGIVAF
                     IEANDLARDQLDQIQQYISAFPDVEPRTAAQAFIVLNFMAYFLLIPAMVPMAIATQSV
                     IGEKVARSLEPQLASPMEVPELLAGKTLAAATPAILATWAVFVGYGLLNGAIADPRLT
                     RFVFSDVWIVAMLTLVPLICMLSVLLGIVVSARVNDPRTAQQIGGFVVIPIIAVAVAQ
                     FFGGQPTFTMQQVLIGDLVVAALIGAAMVLGSWAFERESILSRLA"
     gene            complement(959..1921)
                     /locus_tag="VHK28_10945"
     CDS             complement(959..1921)
                     /locus_tag="VHK28_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142768.1"
                     /translation="MIVVEGLRKEFGRLVAVHDLSFTVADGEIFGLLGPNGAGKTTTL
                     RMLAGLIGPTAGSATVNGHPLGSGSQHIRVTTGILTESPGLHDRLTARQNLAYYGRLY
                     GLRGGTLRAAVDRYLGVVGMAEHADRRVSGFSKGMRQKIAIARALLHEPEVIYLDEPT
                     SGLDPSAAKTVRDFIATLRGLGRSIVVCTHNLDEAERLSDRIGIMNGSLLRVDTPAGL
                     RRHGRNAIVKVELNGARGPQSFIDLLAGLPYVLGAMPHDDDRSLHVELGDPAGDTPDL
                     VATLVGAGARITGVREESATLEEVYLGLVGEAGERDAPRSASAQ"
     gene            complement(1971..2807)
                     /locus_tag="VHK28_10950"
     CDS             complement(1971..2807)
                     /locus_tag="VHK28_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142769.1"
                     /translation="MSLAQPSILEARDLVKVYRRGASEVRAVDGISLTVAAGEFMSVM
                     GRSGSGKTTLLDLLGCLLRPTSGELYVDARRVTGAADAELARVRQSRIGFVFQEFNLI
                     PTLNALENVMLPLRYGPRRRDARTRATSLLALVGLGERLRHRPSELSGGEQQRVAIAR
                     ALVNDPAVVLADEPTGELDSTTSSSLLAMLRQLNHDRGVTFVVVTHDPGVAGATDRIV
                     RMSDGRVVADVRRDDRGWVEALGLGAVGPAVRDGDGGRPVWARLGALLSRTRTTARAG
                     ER"
     gene            complement(2804..3967)
                     /locus_tag="VHK28_10955"
     CDS             complement(2804..3967)
                     /locus_tag="VHK28_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2142770.1"
                     /translation="MSEAIRNILRRKLRTGLTILGIVIGVFALTVMGALAEKLNVLVD
                     GGDEYFRTRIFVFDQSAASGFQASARPITRDLVERIETVDGVQRATPVLQTLLDPDEI
                     GGFDIPELLTGIEPETWYDASVDLRVDAGRLLEEGERGVTVIGADLAERYDRRVGDDF
                     EVRGRTFEVIGVLERTLTFPDKIAYVPLRDAQEIYVETVPQGFDYRTDELATQIEVFP
                     HDLDDADGVAAAIEEEIAQVRTVTPEQVADQIGQASVVFNLIIVGASVIAVIVGGLSV
                     INTMVMTVSERVREIGIKKAVGARTGAILAEFIAEATLLGAIGGLIGLSAGWLLVIIL
                     NAQTAGSGTTVFLVTPALLLRSFVFATVLGALAGIYPALRAARLDPVQALRST"
     gene            4124..4669
                     /locus_tag="VHK28_10960"
     CDS             4124..4669
                     /locus_tag="VHK28_10960"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2142771.1"
                     /translation="MTDSERITSTRRAYEGRLINLRVDEVALPSGRTAQREVVEHPGA
                     VGILAWDGGGLAMVRQWRHAVGEPLLEIPAGTLDADEEPLATARRELAEECSLAAETW
                     ERGPSFYTAPGFCTERLALYLATGLHDADGQSDDDEDLELSWMSLPEALAAVDDGRVA
                     DAKSIAGVLWLARRIGQPSPA"
     gene            4692..5789
                     /locus_tag="VHK28_10965"
     CDS             4692..5789
                     /locus_tag="VHK28_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX2142772.1"
                     /translation="MATRAASPPSNGAESAVEAGLRYVSDAQPGITRRRAGKGFSYRD
                     PDGRRITDRSVLERIRRLAIPPAWTDVWISPIPSGHLQATGRDAKGRKQYRYHARWRA
                     ARDEEKYERMLAFGRALPKIRRRVEEDLSLPGMPRERVLAAVVRLLEKTRARVGNEEY
                     ARDNRSYGLTTLRNHHARVGGSRIRFRFRGKGGKVSDLELTDKRLARIVARAQDLPGQ
                     QLFTYLDENGEPRTVDSADVNDYLRQITGRDFTAKDFRTWAGTVLAAWALSEFEDVDS
                     AAQKKKHVVRAVEDVAERLGNTPAVSRASYVHPTVIDAYLDGDVVRAARESADRELAE
                     SLDDLSPQEAAVLALLRRRLRDEERASRGRG"
     gene            complement(5786..6184)
                     /locus_tag="VHK28_10970"
     CDS             complement(5786..6184)
                     /locus_tag="VHK28_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142773.1"
                     /translation="MPPDAKRSQGPDDERESAYELFQRGSRMLAEHHPGAAAVLLERC
                     LALEPGKASILEALARAYYNQGDHLHAAERFQEMVDANPLADYAHFGLGLSRARLGDR
                     RAAGRHLRMAVFLQPGNEDYQRALRRLDAA"
     gene            complement(6189..7298)
                     /locus_tag="VHK28_10975"
     CDS             complement(6189..7298)
                     /locus_tag="VHK28_10975"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX2142774.1"
                     /translation="MPCRRTGRRPPAGNGDGPGACRSWRSSVDAGRARSAPAADVLSG
                     DTADVYFDRAQRILAAEQMDPVVTMEIFPREDAILCGAEEALAYLREVLGDGRRFEPD
                     PVVESLHDGDAIGRKEVVLRITSRYSTFGLYETAILGILSQSTGWATAARRIVDLAEP
                     IPVIGFGARHVHPSVADQMDYASVVGGCIGASTPAGARLAGLAPTGTMPHALILIFGD
                     TVRAMEAFDRHIDPDVPRIALVDTFKDEAEEAVRVADALGERLWGVRLDTPAERGRVT
                     ADLVKEVRARLDMAGHRHVKIVVSGGLDAERIVYFKEAAAPIDSFAVGSAISDASPID
                     FTGDLKEIDGNPIAKRGRIPGRTSNPRLERVDLRA"
BASE COUNT         1145 a         2646 c         2557 g         1046 t
ORIGIN      
        1 accatccgtg agcgtgaaac gctgtcgcaa ggcacgccga aaggttcgag atgatctcca
       61 acgcgactgg tggtcgcgcc tgaggggggt tagcccatcc gagccggata gcgcgactgg
      121 tggtcgcgcc tgaaaggatc gggctgacgc acaacaggcc agccatcggg ctatgccagg
      181 cggctgagga tcgactcccg ctcgaaggcc cacgagccga gcaccatggc ggcgccgatc
      241 agagcggcca ccaccaggtc gccgatcagc acctgctgca tggtgaacgt cggctgcccc
      301 ccgaagaact gcgcgaccgc caccgcgatg atggggatca ccacgaaccc gccgatctgc
      361 tgggccgtgc gcgggtcgtt gacgcgcgca ctgaccacga tccccagcag aacgctgagc
      421 atgcagatga gcgggaccag ggtcagcatg gccacgatcc acacgtcgct gaacacgaag
      481 cgcgtcagcc gcggatcggc gatggcgccg ttcagcaggc cgtagccgac gaagaccgcc
      541 caggtggcca ggatggccgg cgtggccgcc gccagggttt tgccggccag gagctccggc
      601 acctccatcg ggctggcgag ctgtggctcc agggagcggg ccaccttctc gccgatcacc
      661 gactgggtgg cgatggccat gggcaccatg gccgggatca acaggaagta ggccatgaag
      721 ttaaggacga tgaatgcctg ggcggccgtc cgcggctcga cgtccgggaa ggcgctgatg
      781 tactgctgga tctggtccag ctggtcgcgc gccaggtcgt tggcctcgat gaatgcaacg
      841 atcccggttg ggatggcggc gaagatcagg gccggtaaca ccacggcgcc cagcagcagc
      901 cggttgctca gcaggtcgcg ccactccttg ccgaagaccg cccagacgta acccggcctc
      961 attgggccga tgccgagcgc ggcgcgtcgc gctcccccgc ctcgccgacc agccccaggt
     1021 agacctcctc cagggtggcg gactcctcgc gcacgccggt gatccgcgcc ccggcgccca
     1081 ccagtgtcgc gaccaggtcc ggggtatcac cggcagggtc gccgagctcg acgtggaggg
     1141 accggtcgtc gtcgtgcggc atggcgccca ggacgtacgg cagcccggcc agcaaatcga
     1201 tgaacgactg cggcccgcgc gccccgttga gctccacctt gacgatcgcg ttccttccgt
     1261 gccgccgcag gccggccggc gtgtccacgc gcagcaggct gccattcatg atcccgatgc
     1321 ggtcgcttag gcgctccgcc tcgtccaggt tgtgagtgca caccacgatg ctgcgaccca
     1381 gcccgcgcag ggtggcgatg aagtcgcgca ccgtcttggc cgccgacggg tccaggccgc
     1441 tggtcggctc gtcgaggtag atcacctccg gctcgtggag cagggcccgc gcgatggcga
     1501 tcttctggcg catgcccttg ctgaagccgc tcacgcggcg atccgcgtgc tcggccatgc
     1561 cgacgacgcc caggtagcgg tccacggcgg cgcgcagggt gccaccccgc agcccgtaga
     1621 gccgaccgta gtaggccagg ttctggcgcg cggtcaggcg gtcatgaagg cccgggctct
     1681 cggtgaggat gccggtggtg acccggatgt gctgcgagcc cgagccgagg ggatggccgt
     1741 tcacggtggc gcttcccgcg gtcggcccga tcagcccggc cagcatgcgc agcgtcgtgg
     1801 tcttgccggc cccgttcggg ccgagcagtc cgaagatctc cccgtcggcg accgtgaagc
     1861 tcaggtcgtg gaccgccacc agccggccga actccttgcg cagcccctcg acgacgatca
     1921 ctcggtcagc ctccccagtc gcaccctccc gctccgggcg tcgcccgatc ctagcgctcg
     1981 ccggcgcgtg ccgtggtgcg cgtgcgcgac aggagcgccc cgaggcgcgc ccataccggt
     2041 cgcccgccgt caccgtcccg tactgccggg ccgaccgcgc cgaggccgag cgcctcaacc
     2101 catccccggt cgtcgcggcg cacgtcggcg accacccgcc cgtcgctcat gcgcacgatg
     2161 cggtcggtgg cgccggccac gccggggtcg tgcgtcacca ccacgaaggt cacgccccgg
     2221 tcgtggttga gctggcgaag catggccagc agggaggacg aggttgtgct gtccagctcg
     2281 ccggtcggct cgtcggcgag cacgacggcc gggtcgttga ccagcgcccg ggcgatagcc
     2341 acccgctgct gctccccgcc tgacagctcg gacgggcggt gccgcagccg ctcgccgagg
     2401 ccgaccagtg ccagcagcga ggtggcgcgc gtgcgggcgt ccctgcgccg cggcccgtag
     2461 cgcagcggca gcatgacgtt ctccagcgcg ttcagggtcg ggatcaggtt gaactcctgg
     2521 aagacgaacc cgatgcgcga ctgccgcacc cgcgccagct ccgcgtcggc cgcgccggtc
     2581 acgcggcggg cgtccacgta cagctcaccc gacgtcgggc gcagcaggca gcccagcagg
     2641 tccaggagcg tggtcttgcc gctccccgat ctgcccatga cggacatgaa ctcgccggca
     2701 gcgaccgtca ggctgatccc gtccacggcg cgtacctccg acgccccgcg gcggtagacc
     2761 ttgaccaggt ctcgcgcctc caggatcgac ggctgggcga ggctcacgtg cttctcagcg
     2821 cctggaccgg gtccagacga gcggcacgca gcgccgggta gatgccggcc agcgccccga
     2881 gcacggtcgc gaagacgaac gagcgcagca gcagcgcggg cgtgaccagg aagacggtgg
     2941 tgccgctccc cgcggtctgc gcattcagga tgatgaccag cagccagccg gcgctcaggc
     3001 cgatcaaccc gccgatcgcc cccagcagcg tggcctccgc gatgaactcg gccaggatgg
     3061 cgcccgtcct ggcgccgacc gccttcttga tgccgatctc ccggacccgc tcgctcaccg
     3121 tcatgaccat ggtgttgatc accgagagcc cgcccacgat gacggcgatg acgctggcgc
     3181 ccacgatgat caggttgaac acgacgctgg cctggccgat ctggtcggcc acctgctccg
     3241 gcgtgacggt gcgcacctgg gcgatctcct cctcgatggc tgccgccacc ccatcggcat
     3301 cgtccaggtc gtgggggaag acctcgatct gggtggccag ctcatcggtc ctgtagtcga
     3361 agccctgcgg gaccgtctcg acgtagatct cctgcgcgtc gcgcagcggg acgtacgcga
     3421 tcttgtcggg gaaggtcagc gtgcgctcca gcacgccaat cacttcgaag gtgcggccac
     3481 ggacctcgaa gtcgtccccc acccgccggt cgtagcgctc ggccaggtcg gcaccgatga
     3541 ccgtcacccc tcgctccccc tcctccagga ggcgcccggc gtccacccgc aggtcgacgc
     3601 tggcgtcata ccaggtctcc ggctcgatcc cggtcaacag ctcgggaatg tcgaagccgc
     3661 cgatctcgtc cggatccagc agcgtctgca ggacgggcgt tgcgcgctgc acgccgtcaa
     3721 ccgtctcgat gcgctccacc aggtcgcggg tgatcggtcg cgcactggcc tggaagccgg
     3781 acgccgcgct ctggtcgaag acgaagatcc gggtgcggaa gtactcgtcc ccgccgtcga
     3841 cgagcacgtt cagcttctcg gccagcgcgc ccatcacggt gagcgcgaag acgccgatga
     3901 cgatgcccag gatggtgaga cccgtgcgca gcttgcggcg caggatgtta cggatggctt
     3961 cgctcatgga ccgatgcaga tgctagccgt caatcccgtt tcgcgcccgt ttcgtgccgg
     4021 ccaccgagat gttgatgccg cactgtcaga cctcgttgtg ctgcacagcc gcacagttgc
     4081 acaggtgcac gatcgaacgt gacagcgcgg gtagcatcgg tcgatgaccg acagcgagcg
     4141 cattacgagc accaggcggg cgtacgaggg cagactcatc aacctccgcg tggacgaggt
     4201 ggcactgcca agcggacgca ccgctcagcg cgaggtggtg gagcatcccg gcgcggtcgg
     4261 gatcctggcc tgggatggcg gggggctggc gatggtgcgc cagtggcggc atgccgtcgg
     4321 cgagccgctg ctcgagatcc cggccggcac gctggacgcg gacgaggagc cgctggccac
     4381 cgcccgccgt gagctggccg aggagtgctc gctggcggcc gagacctggg agcgaggccc
     4441 gtccttctac acggcccccg gcttctgcac cgagcgcctg gcgctgtacc tggccaccgg
     4501 cctgcacgac gcggatggcc agtcggatga cgacgaggac cttgagctca gctggatgag
     4561 cctgccagag gcgctggcgg cggtggacga cggccgcgtc gcggatgcca agtcgattgc
     4621 cggcgtcctc tggctcgccc gacggatcgg ccaacccagc ccggcatagc gcttgctcag
     4681 agcgggtgcc gatggccacc cgtgcggctt ctccaccctc caacggcgcc gagtcggcgg
     4741 tcgaggccgg tctgcgctac gtcagcgacg cgcagccggg catcactcgc cggcgcgcag
     4801 gcaagggctt ctcgtaccgc gaccccgatg gcaggcgcat cacggatcga tccgtcctgg
     4861 agcgcatccg tcggctggcc atcccacccg cctggaccga tgtctggatc tcacccatcc
     4921 ccagcggcca cctgcaggcc accggtcgcg acgcgaaggg gcgcaagcag taccgatacc
     4981 acgccaggtg gcgagcggcg cgcgacgagg agaagtacga gcgcatgctg gccttcgggc
     5041 gtgcgctgcc gaagatccgc aggcgggtcg aggaggacct ctcactgccc ggcatgccgc
     5101 gcgaacgggt gctggcggcc gtggtgcggc tgctggagaa gacgcgggca cgcgtcggca
     5161 acgaggagta cgcccgcgac aaccgctcgt acgggctcac aaccctgcgc aaccaccacg
     5221 ccagggtggg aggcagccgc atccgcttcc gcttccgggg caagggaggc aaggtgagcg
     5281 acctggagct gaccgacaag cggctggccc gcatcgttgc ccgcgcccag gacctgcccg
     5341 ggcagcagct gttcacctac ctcgacgaaa atggcgagcc gcgcaccgtc gattcggcgg
     5401 acgtcaacga ctacctgcgc cagatcaccg gccgcgactt caccgcgaag gacttccgga
     5461 cctgggccgg aacagtgctg gccgcctggg cgctgagcga gttcgaggac gtggacagcg
     5521 cggcacagaa gaagaagcac gtggtgcgcg ccgtggagga cgtcgccgaa cggctgggga
     5581 acacccccgc cgtctcacgc gcctcgtacg tgcatccgac cgtcatcgac gcctacctgg
     5641 acggcgacgt cgtgcgggcg gcgcgcgagt cagccgaccg cgagctcgcc gaatcgctgg
     5701 acgacctcag tccgcaggag gccgcggtgc tcgccctgct gcgccgccgt ctgcgcgacg
     5761 aggagcgggc atcccgggga cgcggctagg cagcatccag gcgtcgcaac gcccgctggt
     5821 agtcctcgtt gccgggctgg aggaagaccg ccatgcgcag gtgacggccg gccgcccgtc
     5881 gatcgccgag ccgcgcccgt gacagcccga ggccgaagtg cgcgtaatcg gccagcgggt
     5941 tggcgtccac catctcctga aagcgttccg cggcatggag gtgatcgccc tggttgtagt
     6001 aggcgcgggc cagcgcctcc aggatggagg ccttgcccgg ctccagggcc aggcagcgct
     6061 ccagcagcac ggccgccgcc cccggatggt gctccgccag catgcggctg ccgcgctgga
     6121 acagctcgta ggcgctctca cgctcgtcgt cggggccctg ggatcgtttg gcgtcgggag
     6181 gcatcggtct acgcgcggag gtccacgcgt tccaggcgcg gattcgaggt cctaccggga
     6241 atgcggcccc gcttggcgat ggggttgccg tcgatctcct tgaggtcgcc ggtgaagtcg
     6301 atggggctgg cgtcgctgat ggccgagccc acggcaaacg agtcgatggg tgccgccgcc
     6361 tccttgaagt agacgatccg ctccgcgtcg aggccgccgg agacgacgat cttcacgtgc
     6421 cggtgaccgg ccatgtcgag gcgagcccgg acctccttca ccaggtcggc agtcacccgc
     6481 ccgcgctcgg cgggtgtgtc gagccggacg ccccacaggc gctcgccgag cgcatccgcc
     6541 acgcgcacgg cctcctccgc ctcgtccttg aacgtgtcga ccagggcgat gcgcggcaca
     6601 tcagggtcga tgtgccggtc gaaggcctcc atggcccgga ccgtgtcccc gaagatcagg
     6661 atcagcgcgt gcggcatggt tccggttggc gccaggcccg caagccgcgc accagccggt
     6721 gtcgaggcac cgatgcaacc gcccaccacg ctggcgtagt ccatctggtc cgccaccgag
     6781 gggtgcacgt gccgcgcgcc gaagccgatc accgggatgg gttccgccaa atccacgatg
     6841 cgccgggcgg cggtggccca ccccgtggac tggctcagga tccccaggat cgcggtctcg
     6901 tacaggccga aggtcgagta gcgagaagtg atgcgcagca cgacctcctt gcggccgatc
     6961 gcgtcaccgt catgcagcga ctcgaccacc ggatcgggct cgaatcgccg gccatccccc
     7021 agcacctcgc gcaggtaggc cagggcctcc tccgccccgc acaggatggc gtcctcgcgc
     7081 gggaagatct ccatggtcac caccgggtcc atctgctcgg cggccaggat ccgctgcgcc
     7141 cggtcgaagt agacgtctgc cgtgtcgccc gacaggacgt cggccgccgg cgccgaacgc
     7201 gcccggccag cgtctacact gctgcgccat gaccgacacg cgcccggccc gtccccgttt
     7261 ccagccggcg gccgacgtcc tgtccggcga cacggcagac gtctacttcg accgggcgca
     7321 gcggatcctg gccgccgagc agctggaccc ggtggtgacc atggagatct ttccgcgtga
     7381 ggacgccatc ctgt
//
LOCUS       DATACA010000542         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2163983, whole genome shotgun sequence.
ACCESSION   DATACA010000542 DATACA010000000
VERSION     DATACA010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163983"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VHK28_10980"
     CDS             complement(<1..350)
                     /locus_tag="VHK28_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX2142775.1"
                     /translation="MHPLAWLALAIGTEVVATTALKLSDGFTRLGWVAVVVLGYGVSF
                     YALSVALRTIPLGVVYAVWSGVGTATVVVIGYFLFGEVLDALKLGAIAMIVAGVFLLH
                     VGGGAASGPAGGGAA"
     gene            complement(363..1070)
                     /locus_tag="VHK28_10985"
     CDS             complement(363..1070)
                     /locus_tag="VHK28_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX2142776.1"
                     /translation="MDVSLALAFAAGVLSFVSPCVLALVPVYLAFLGETAAVTASGPR
                     LQGSRGAVLGQAALFVAGFSLLFVVLGVSAGLLGSYLFRADEARVVAGVIVIGLGLLT
                     TGVFGPVLDRWRIGLDPGVLPATRSARSLGLGALVAIGWTPCIGPVLGTILTMGASSQ
                     QAPAAALLLVAYSAGLALPFLAAAVALPRMQPVLATLRRHASVVQVVSGIFIMAVGVL
                     ILTNAFSVLAGWFTFFI"
     gene            complement(1078..>2494)
                     /locus_tag="VHK28_10990"
     CDS             complement(1078..>2494)
                     /locus_tag="VHK28_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142777.1"
                     /translation="GLYLAGAGTAVAASFVVSVIAVRPAGPVPSYPTVATNRALARVA
                     SVLLGVLGLAWWFGAILAGYLLDAVSPLPAILFWIGMWVGLPITAVLLGNPWPSLSPF
                     RTVFGLLERGAGALGARRLDLGLPYPTVLGRWPAVVLLFVALWCELVLFNSDSPRLIA
                     NLLLGYTVLTMAGMLLFGRIAWLRNAELFEVLLGWFGRVGPLGRRAVDPEVCQGCLDR
                     CDPDRCIDCPECSVAAEPRERRAELRPWFVGLTEVRAAGWSDAAFIVLALGGVTYDGF
                     KETAFYGTVAQPAFELALDVLGPADAVLTVQTAGLLVVWLGFLAAFGIAGWVTRALHD
                     PDPRPQPLATTAGAYAATLLPIAGGYLVAHYLTVVIQGVVWVPMLLTNPLNTVAPPLD
                     WIPTGAIWYLSVGAIVLGHIVAVVLAHRLALRDAPGRAVLAGLPLVLLMIGYTVLSLW
                     IIAAPITVDPGTPAPAALRRP"
BASE COUNT          484 a          889 c          893 g          228 t
ORIGIN      
        1 gccgccccgc cccccgcagg tcctgaggcg gcaccgcccc cgacgtgaag caggaacacc
       61 ccggccacga tcatggcgat ggcacccagc ttgagcgcgt ccagcacctc gccgaacagg
      121 aagtagccga tcaccaccac cgtcgccgtg cccaccccgg accacaccgc gtagacaacg
      181 cccagcggga tggtgcgcag ggcgaccgac agggcgtaga agctgacccc gtagccgagc
      241 accacgacgg ccacccaacc gagtcgggtg aagccgtcgg agagcttgag cgcggtggtg
      301 gccacgacct cggtgccgat ggccagcgcc agccaggcga gcggatgcat gcgcgtacct
      361 cgtcagatga agaacgtgaa ccagccggcc agaacgctga aggcgttggt caggatgagc
      421 acccccaccg ccatgatgaa gatcccggag acgacctgga ccacggacgc gtggcgtcgc
      481 agggtggcca gcaccggctg catgcgtggc agggcgacgg ccgcggccag gaagggcagc
      541 gccaggccgg ccgagtaggc caccagcagc agggcagcgg caggcgcctg ctgcgaggag
      601 gcgcccatgg tcaggatggt gcccaggact ggaccgatgc acggcgtcca gccaatggcc
      661 accagcgccc ccagccccag cgaccgtgcg gagcgggttg ccggcaggac gccgggatcc
      721 agaccgatgc gccatcggtc gaggacgggc ccgaacacgc cagtggtcag cagcccgaga
      781 ccgatcacga tcacgccggc caccacccgc gcctcgtccg cgcggaacag gtagctcccc
      841 agcaggccgg cagagacacc gagcaccacg aacagcaggc tgaagccggc cacgaacagg
      901 gcggcctgcc cgaggacagc accccgggat ccctgcagac gtgggccgga ggcggtgacc
      961 gccgccgtct cccccaggaa ggcgaggtag accgggacca gggccaggac gcagggggag
     1021 acgaagctca gcactccagc ggcgaaggcg agggccagag agacgtccat cggtgagtca
     1081 gggacggcgc agggcagccg gcgcgggcgt gccgggatcg acggtgatgg gcgccgcaat
     1141 aatccacagc gacaggacgg tgtagccgat catcagcagc accagcggca gcccggccag
     1201 cacggcgcgg ccgggcgcgt cgcgcagcgc gagccgatgg gccagcacca ccgccacgat
     1261 gtggcccagc acgatggcac ctaccgacaa ataccagata gcgccggtcg ggatccagtc
     1321 cagcggcggc gcgacggtgt tcagcgggtt ggtcagcagc atcggcaccc agaccacgcc
     1381 ctggatcacg accgtgaggt agtgcgccac caggtagccg ccggcgatcg gcagcagggt
     1441 ggcggcgtag gcgccggccg tggtggccaa aggctgcgga cgcggatcag ggtcgtggag
     1501 tgcgcgcgtc acccagccgg cgatgccgaa ggcggccaaa aacccaagcc agacgacgag
     1561 cagccccgcg gtctgcaccg ttagcacggc atccgccggc cccaggacgt cgagcgccag
     1621 ctcgaaggcg ggctgcgcga ccgtgccgta gaaggcggtc tccttgaagc cgtcgtaggt
     1681 caccccgccc agcgccagga cgatgaatgc ggcgtccgac cagccggcgg cccgcacctc
     1741 ggtgagcccg acgaaccagg ggcgcagctc ggctcggcgc tcgcgtggct cagcggccac
     1801 cgagcactcc gggcagtcga tgcagcggtc cgggtcgcat cggtccaggc agccctggca
     1861 gacctcgggg tccactgcgc ggcggcccag agggcccacc cgaccgaacc agcccagcag
     1921 cacctcgaag agctcggcat tgcgcagcca ggcgatgcgc ccgaagagga gcatgccggc
     1981 catggtcagc acggtgtaac cgagcagcag gttggcgatc aggcgcgggc tgtcgctgtt
     2041 gaacaggacg agctcgcacc acagggccac gaacagcagg acgaccgccg gccagcgccc
     2101 gaggacggtc gggtagggca atcccaggtc aagccggcgc gcgccgagcg ctccggcacc
     2161 ccgctccagc agcccgaaca cggtccggaa cgggctgagc gatggccacg ggttgccgag
     2221 cagcaccgcg gtgatcggca gccccaccca catcccgatc cagaacagga tggccggcag
     2281 cggcgacacg gcgtcgagca ggtagccggc caggatggcg ccgaaccacc aggccagccc
     2341 cagcactccc agcaggaccg atgcgacccg tgccagcgcc cgattggtcg cgaccgttgg
     2401 gtaggacgga accggtccgg cggggcggac ggcgatcacc gagaccacga acgaggcggc
     2461 caccgccgtg ccggcgccgg ccaggtagag cccc
//
LOCUS       DATACA010000543         4251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2645398, whole genome shotgun sequence.
ACCESSION   DATACA010000543 DATACA010000000
VERSION     DATACA010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2645398"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VHK28_10995"
     CDS             complement(<1..1291)
                     /locus_tag="VHK28_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2142778.1"
                     /translation="MIPVAFTDPAWLWLLVPAVGVVVAGWLLASRTLPRARRGASLVI
                     RLVLVACLVGSLAGARLTLPSDRLSVVFLVDASASMVDATREELLTFARDATRRMPQG
                     DTAGVVVFGANALVDRLPSDVDQLPPPSSIPVVGATDVAAAVRLAEAIFPAGVQRRLV
                     LLSDGNDTGGGGGQAVAAAAARGVRLDVVLPDRATYPEALVDGVDAPAGARVGEAVEL
                     VVRVRSTIETAATLRLLADGATVGTRELDLEPGARSVSFSVEADAAGFHVFRAVLEPA
                     DDHFVENNAADAYVLVSGEPQVLLATDDSGRAADLLEALEEARHNVTVVPAGGVPSSL
                     ATLAGYDAILLDNVSAAELGPTTMASLQVYVRDLGKGLVMLGGRDSYGAGGYLNTAME
                     EILPVYMTVRDRERSPDVALVAVVDQSGSMADCHCTGD"
     gene            complement(1288..3276)
                     /locus_tag="VHK28_11000"
     CDS             complement(1288..3276)
                     /locus_tag="VHK28_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HEX2142779.1"
                     /translation="MITPLALIGLVSLPIIVAFYMLRLRRRDVPVGSTFLWQQLVRDV
                     EANAPWQRLRFSWLLLVQLLIALLAVGAAVRPFATASSDLAANVVLIIDTSASMGAID
                     PEGERMAQAREAARAVIGEVPDAGRLTVVAADDSAQVLVSETDDRAAALAAVDGIQAT
                     EVPGDLTDAFALASALAARDSDSSVVVVTDANADRLPRVGIGAPIRVERVGSTDDNQA
                     IAALSAIRRSGGSQVDLFVAVSNPSSSEISRRLEVYADGVLVDARDLTLPAEQRTEAL
                     IASVPPSAFLVEARLDGEDALAVDDSALALVPSEEVVPALLVSDGNTYLENALALLPR
                     LELYAVGPAGYEEAIEAGTEDGRPYGMVVFDRFVPDAPPPLPTLWIGPDQDGPFGTVG
                     AAVDGPALAPIAADDPLLRFLDLSTLHVGRARAVELADGMRAAVSSTEGEPLIAAGRV
                     DGRTQALLAFELGETDLPLQVAFPLLVSSLADFLLPPTDGILPPSVDLGEALVVQLNP
                     AISRVRMDTTPVNRDPGSAGQALELDVVGGRVTLPGARRPGVVRLSALDGLAEGADPV
                     LGTTAANLFDPGESRVAPGDPLRISDLGRVGPGTGGEPGTARNEWWWPLALVALLLLL
                     VEWVLFHRPSRRAAARLLGRGGRGAAPSVSRPAGGTTR"
     gene            complement(3456..>4251)
                     /locus_tag="VHK28_11005"
     CDS             complement(3456..>4251)
                     /locus_tag="VHK28_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX2142780.1"
                     /translation="MKGERRSVRRGQSVEFADYRNYTAGDDLRQLDWNVYARLERLFI
                     KLFVEEEDVTVHVLVDASRSMDFGAPNKLWFARRAAGALAYLGLAHLDRVSVGFLGDG
                     RATILRPIRGKARVLEVFRFLSEPRSERLTGLAPAAREYAARLRGRGPMILISDLMDP
                     GYLDALRDLAGTRCQLSVLHVLAPEELDPEVPPDARLVDNESGAGVDVTGDDDLVERY
                     RTRLAEWQAEIGQFVSRRGGSYVTVPSDLDLADLLFDTLRRRRVLE"
BASE COUNT          625 a         1575 c         1539 g          512 t
ORIGIN      
        1 cgtcgccggt gcagtggcag tccgccatgc tgccgctctg gtcgaccacg gccaccaggg
       61 ccacgtccgg ggagcgctcc cggtcgcgga ccgtcatgta gaccgggagg atctcctcca
      121 tggccgtgtt gaggtagccg ccggccccgt agctgtcgcg gccgccgagc atgaccagcc
      181 ccttgccgag gtcgcgcacg tagacctgca ggctggccat ggtggtcggt cccagctcgg
      241 cggcggacac gttgtccagc aggatggcgt cgtagccggc cagcgtcgcc agcgacgacg
      301 gaacgccgcc tgcgggaacc acggtcacgt tgtgccgcgc ctcctccagt gcctcaagca
      361 ggtccgccgc tcgcccggag tcatcggtgg ccagcagcac ctgcggctcg ccgctgacca
      421 gcacgtaggc atcggccgcg ttgttctcca cgaagtggtc atccgcgggc tccaggacgg
      481 cgcggaagac gtggaagccg gccgcatcgg cctcgacgct gaaggagacg ctccgcgcgc
      541 cgggctccag gtccagctcg cgggtgccga ccgtggcgcc atcggccagc aggcggaggg
      601 tggcggccgt ctcgatggtc gagcgcaccc gaaccaccag ctcgacggcc tcgcccacgc
      661 gcgcgccggc gggcgcatcc accccgtcca ccagggcctc cgggtaggtg gcgcggtccg
      721 gcagcacgac gtccagccgc acgccacggg ccgcggccgc cgccaccgcc tgcccgcctc
      781 cgcccccggt gtcgtttccg tccgagagca ggaccaggcg tcgctgcacc ccggccggga
      841 agatggcctc cgccagccgc accgccgcgg cgacgtcggt ggcgcccacc accgggatgg
      901 aggacggcgg cggaagctgg tccacgtcgg agggcagccg gtctaccagt gcgttggcgc
      961 cgaacacgac cacgcccgca gtgtcgccct gtggcatgcg ccgtgtcgcg tcgcgcgcga
     1021 aggtcagcag ctcctcgcga gtggcgtcca ccatggaggc ggaggcgtcg accaggaaga
     1081 cgacggacag gcggtcggag gggagcgtga ggcgggcgcc ggccagggag ccgaccaggc
     1141 acgccaccag gaccagcctg atgaccagcg aggcaccgcg ccgggcgcgt ggcagcgtcc
     1201 tcgaggcgag cagccagccg gccaccacca caccgacggc tggcaccagc agccacagcc
     1261 atgccggatc ggtgaaggcg actgggatca ccgcgtcgtg ccccctgccg gccgcgagac
     1321 cgatggagcc gcgccccgtc cgccacggcc cagcaggcgc gccgctgcgc ggcgcgaagg
     1381 gcggtggaac aggacccact cgaccagcag cagaagcagc gcgaccaggg ccagcggcca
     1441 ccaccactcg ttgcgggcgg tgcccggctc gccgccggtg cccggcccga cccgccccag
     1501 gtcgctgatg cgcagcggat caccgggcgc cacccgcgac tcgcccgggt cgaacaggtt
     1561 cgcggccgtc gtgccgagca ccggatcggc gccctccgcc agcccgtcca gcgcgctgag
     1621 gcgcaccacg ccgggacgcc gcgcgccggg cagcgtcacc cggccaccga ccacatcgag
     1681 ctcaagtgcc tgtcccgcgg aaccgggatc ccggttcacc ggcgtggtgt ccatgcggac
     1741 gcggctgatg gcagggttca gctgcaccac cagcgcctcg cccaggtcca cggagggcgg
     1801 gaggatgccg tcggtcggcg gcagcaggaa gtcggccagg ctgctgacca gcagcgggaa
     1861 cgcgacctgc agcggcaggt ccgtctcgcc gagctcaaag gcgagaagcg cctgcgtgcg
     1921 cccgtcgacg cgcccggcag cgatcagcgg ctcgccctcg gtgctcgaga cggcggcccg
     1981 catgccgtcc gccagctcca cggccctcgc gcggcccacg tgcagggtgg agaggtccag
     2041 gaagcgcagc agcgggtcgt ccgcggcgat cggcgccagc gcaggcccat ccaccgcggc
     2101 gcccacggtg ccgaacgggc cgtcctggtc cggaccgatc cacagcgtgg gcagcggcgg
     2161 cggcgcgtct ggcacgaagc gatcgaagac gaccatcccg tacggccggc cgtcctccgt
     2221 gccggcctct atcgcctcct cgtatccggc cggacccacc gcgtacagct ccagacgggg
     2281 cagcagggcc agggcattct ccaggtacgt attcccgtcg ctgaccagga gcgccggcac
     2341 gacctcctcc gacggcacga gtgccaatgc cgagtcgtcc acggccaggg cgtcctcccc
     2401 gtcgagccgc gcctcgacca ggaaggccga gggcgggacg ctggcgatca gcgcctcggt
     2461 gcgctgttcc gcgggcagcg tcaggtcgcg ggcgtcgacc agcacgccat cggcgtagac
     2521 ctccaggcgg cggctgatct cgctgctgga cgggttgctg acggccacga acaggtcgac
     2581 ctgcgaaccg ccggaacgcc ggatggcgga gagggcggcg atcgcctggt tgtcatcggt
     2641 cgagccgacc cgttcgaccc tgatcggtgc accgatgccg acgcgcggga ggcggtcggc
     2701 gttcgcatcg gtcaccacca ccacgctgga gtccgagtcg cgagcggcca gcgcagaggc
     2761 cagggcgaag gcatcggtca ggtcaccggg cacttccgtg gcctggatgc cgtctacggc
     2821 cgccagggcc gccgcgcgat catcggtctc gctgaccagc acctgggccg agtcgtcggc
     2881 ggcgaccacg gtcagccgac cggcatccgg tacctcgccg atcacggcgc gcgccgcctc
     2941 gcgcgcctgg gccatgcgct cgccctccgg gtcgatggcg cccatggagg ccgaggtgtc
     3001 gatgatgagc accacgttgg ccgccaggtc ggagctggcc gtggcgaagg gccgtaccgc
     3061 ggcacccacc gccagcagcg cgatcagcag ctgcaccagc agcagccagc tgaagcgcag
     3121 ccgctgccac ggggcgttgg cctcgacgtc gcgcaccagc tgctgccaca ggaaggtgct
     3181 gcccaccggc acgtcgcggc gccgaaggcg gagcatgtag aaggcgacga tgatcggcag
     3241 gctgaccaaa ccgatgagcg ccagcggagt aatcactgga ccgctgcctc cggggcgttg
     3301 tggcgggcgg gacccatccc actcgccgct ggcgcaaggc tcgcgggacg ggccccgccc
     3361 gcctggacat cacggcggca gcgggccacc catctcttcg tagccgttcg tcgggaaggt
     3421 cgccttggat ggggtggtgg cgtgttcatc gctgcctact cgaggacgcg gcggcggcgg
     3481 agggtgtcga agagcaggtc ggccaggtcc aggtccgagg ggaccgtgac gtacgacccg
     3541 ccgcgccgcg agacgaactg gccgatctcg gcctgccact ccgccagccg ggtccggtag
     3601 cgctccacca ggtcgtcgtc gccggtcacg tccacgccgg caccggactc gttgtcgacc
     3661 aggcgcgcgt cgggcggtac ctcggggtcc agctcctcgg gagccagcac gtggaggacg
     3721 gagagctggc agcgggtgcc ggccaggtcg cgcagcgcgt ccaggtagcc ggggtccatg
     3781 aggtcgctga tcaggatcat gggtccgcgg ccgcggaggc gagcggcgta ctcccgcgcg
     3841 gccggtgcca gcccggtgag ccgctcggac cgtggctcgg acaggaagcg gaagacctcg
     3901 aggacgcgcg ccttgccgcg gatgggccgc aggatggtcg cccgtccgtc gcccaggaac
     3961 ccgacgctca cccggtccag gtgcgccagg cccaggtacg ccagtgcccc ggccgcgcgg
     4021 cgcgcgaacc acagcttgtt cggcgcgccg aagtccatgg agcggctggc gtcgaccagc
     4081 acgtggacgg tgacgtcctc ctcctccacg aagagcttga tgaacagccg ctccagccgc
     4141 gcgtagacgt tccagtccag ctgccgcagg tcgtcgccgg ccgtgtagtt gcggtagtcg
     4201 gcgaactcca cgctctgccc gcgccgcacg ctgcgccgct cgcccttcat g
//
LOCUS       DATACA010000544         4243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2405847, whole genome shotgun sequence.
ACCESSION   DATACA010000544 DATACA010000000
VERSION     DATACA010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405847"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="dprA"
                     /locus_tag="VHK28_11010"
     CDS             <1..795
                     /gene="dprA"
                     /locus_tag="VHK28_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX2142781.1"
                     /translation="EVSALEAPCVAVVGTRRASGYGRSAANEIADELARAGVTVVSGL
                     ALGVDGEAHAAAVNAGGRSVAVLPSPLDRIYPPRHRSLASRLVSSGGALLTELAPGSV
                     TGRPDFARRNRIIAGLSRAVVVVEAPDRSGALLTAAAAVDLGREVYAVPGPIDAIASR
                     GCNRLIADHVAAIVTSAPGLVASIGLRRGEKPPSVASLSDPEGLVLGTLIKRPGSLEE
                     LMTRTQLGTSALASALTLLEARGLVTNYGGVTFHPTLQARRIGTVR"
     gene            913..2559
                     /locus_tag="VHK28_11015"
     CDS             913..2559
                     /locus_tag="VHK28_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2142782.1"
                     /translation="MHSIQKIRAALTAATTALLPLIRGHRAITRLALAALAIAVAVGA
                     SLQGASAGPQPAPTFPTSILPENVGVGVQTSDPISLTFPQPMDPASVAPVLAVRPAAE
                     VNLRWSHDGRTLQVVPEDRWRTDARYVVSVDGSARTSSGEELGAARQASFTTQTAPTI
                     SDFQLRYVGQSDEQRMRAMELTVAAPEDAADLPPPGDTATGVSAGTSITMGFSAAMNR
                     RDVERRFVISPDVAGDLAWEGNNLIFTPSERLVPDARYAVSVVGARDAQGNRLNGDVS
                     FSFTTQVAAQVVKVSPGKGKKDVTDRAASVWFSRPMDTTATKLTAVDVTNGAKLGGKA
                     SWNAEGTQLAFAFADPLKAGHTIRMSLAPGALDEDRNEVTLSWTFAVKAAPAPAPAPA
                     QSATTRSAPPPPPPGGGAPAPADLQLYALQQINASRAQYGFAPLVLDSAISAVASAHA
                     WDMLNYGYFSHTGRDGSRVAQRLSRAGIAYSASGENICYHAGIGARATLDWCHRVFMA
                     EPYPGHFNHIANILSPRFSRVGIGIAVKGGSVKVVWDFAG"
     gene            2673..>4243
                     /gene="topA"
                     /locus_tag="VHK28_11020"
     CDS             2673..>4243
                     /gene="topA"
                     /locus_tag="VHK28_11020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX2142783.1"
                     /translation="MPPKKRAAGDVVIVESPAKAKTIERYLGPGYTVLASYGHVRDLP
                     QRSLGVDVDNDFEPRYEPLKDRRRELEKLAKEARHADHVWLATDLDREGESIAWHIAE
                     YADLPADRIRRVTFSEITKSAIDEAFANPRAINQDLVNAQQARRVIDRLVGYKLSPLV
                     SSKIRRGLSAGRVQSVAVRLVVDREREIDAFTPEEYWSIEAQLRRRGSGAGFKAELTR
                     IDGEKAKIGSEKEARAHEAALREAEYVVSRVTRSQQRKNAPAPFTTSTLQQEASRKLG
                     FGAKRTMSVAQSLYEGIALPEGQVGLITYMRTDSVVMASGAVAEAREVIARRHGPEFV
                     PDKPNVFRTKARGAQEAHEAIRPSSFARSPDSLRSSLKPDELKLYALIWRRALASQME
                     PARFDQVGVDITADRYLLHAGARKRIFDGYQALYVEGRDDEDDERLTVLPDLADGEPL
                     DLADLASEQHFTQPPPRYTEATLIRALEEHGIGRPSTYAPTLDTIRQRGYITVKERRL
                     FPEESALRVTDLLKE"
BASE COUNT          664 a         1554 c         1441 g          584 t
ORIGIN      
        1 gaggtcagcg cgctcgaggc gccgtgcgtg gctgtggttg gcacgcgacg ggccagcggc
       61 tacgggcgtt ccgcagcgaa cgagatcgcg gacgagctgg cgcgggcggg ggtcacggtg
      121 gtcagcggcc tcgcactggg agtggacggg gaggcacatg cggccgcggt caacgccggt
      181 ggtcgcagcg tggccgtcct gccctcgccg ttggaccgca tctacccacc acggcatcgg
      241 tcgctggcct cgcgcctggt gagctccggc ggagccctgc tgaccgagct cgcgccggga
      301 agcgtcaccg ggcgcccgga cttcgcgcgc cgcaaccgca tcatcgccgg actgtcgcgg
      361 gcggttgtgg tggtcgaggc gccggaccgc tccggtgccc tgctgaccgc ggcggcggcg
      421 gtcgacctcg gacgcgaggt gtacgccgtg cccggcccca tcgacgcaat cgcctcgcgc
      481 ggctgcaacc ggctgatcgc cgaccacgtc gccgccatcg tcacctcggc gcccggcctg
      541 gtggcctcca tcgggctgcg ccgcggcgag aagccgccat cggtcgccag cctgtcggac
      601 ccggagggcc tggtgctcgg tacgctcatc aagcgccccg gatccctgga ggagctcatg
      661 acccgaactc agctcgggac ctcggcactg gccagtgcgc tcacgctcct ggaggctcgc
      721 ggactggtca ccaactacgg tggcgtcacc ttccatccga ccctccaggc caggcgcatc
      781 ggcacggtcc ggtgagccac cgcgacgtgc accatgacgc cgattcgcag atcgtgtcta
      841 tactgcccgc ggtcgccggc aatccctcgg ggtgccggcc gtaccgtgaa agccacagga
      901 gttcccggtc tattgcattc gatccagaag attcgtgcgg ccctgacggc cgcgaccacc
      961 gccctcctcc cgctgatccg gggccatcga gccatcactc ggctcgcgct cgcagcactg
     1021 gccatcgccg tcgctgtcgg ggcctccctg cagggagcgt ccgccggtcc ccagccagct
     1081 ccaacttttc cgacctccat cctgccggag aacgtcggcg tcggggtgca gaccagcgac
     1141 cccatcagcc tcaccttccc gcagcccatg gatcccgctt cggtcgctcc ggtgctcgcc
     1201 gttcgtcccg cagcagaggt gaacctgcgc tggagccacg acgggcgcac cctgcaggtc
     1261 gtcccggagg atcgctggcg gaccgacgcg cgctacgtgg tcagcgtgga cggcagcgcc
     1321 cgtaccagct ccggcgagga gctcggcgcc gcacgccagg ccagcttcac gacgcagacc
     1381 gccccgacca tttccgactt ccagctgcgg tacgtggggc agtcggacga gcagcgcatg
     1441 cgcgcaatgg agctgaccgt ggcggccccc gaggacgcgg ccgacctccc gcccccgggc
     1501 gacacggcga cgggcgtcag cgccggcacc agcatcacca tgggcttctc ggcagccatg
     1561 aaccgtcgcg acgtcgaacg tcgcttcgtc atcagccccg acgttgccgg cgacctggcc
     1621 tgggaaggga acaaccttat cttcacgccg agcgagcggc tggttcccga cgctcgctac
     1681 gcggtcagcg tggtcggcgc gcgcgacgcc cagggcaacc ggctgaacgg cgacgtctcg
     1741 ttctcgttca cgacccaggt cgcggcccag gtggtcaagg tcagtcccgg caaggggaag
     1801 aaggacgtga ccgaccgggc ggcatcggtc tggttcagtc gtccgatgga taccacggcg
     1861 accaagctca cggcagtcga cgtcaccaac ggggcgaagc tcggcggaaa ggcaagctgg
     1921 aacgcggagg gcacccagct ggccttcgcc ttcgccgatc cgctcaaggc gggccacacc
     1981 atccgcatga gcctggcgcc gggtgccctg gacgaggacc gcaacgaggt gacgctgagc
     2041 tggaccttcg ccgtcaaggc ggcacctgcg cccgctccgg cccccgcgca gtcggccacc
     2101 acccgctcgg cgccacctcc gccaccgccc ggtggcggcg cgccggcccc ggccgacctg
     2161 cagctgtatg cgctccagca gatcaatgcc tctcgggcgc agtacggctt cgctccgctg
     2221 gtgctggact cggccatctc ggcggtggcc tctgcccacg cctgggacat gttgaactac
     2281 gggtacttca gccacaccgg gcgcgatggc tcgcgggtgg cgcagcggct gagccgggcc
     2341 gggatcgcct acagcgcgtc cggggagaac atctgctacc acgccggcat cggcgcgcgg
     2401 gccaccctgg actggtgcca ccgggtgttc atggccgagc catacccggg gcacttcaat
     2461 cacatcgcga acatcctcag cccgcgcttc agccgggtgg ggatcggcat cgcggtcaag
     2521 ggtggctcgg tcaaggtcgt ctgggacttc gccggctgac caccggccgc caggccggga
     2581 tgctgctgga ttccgaaggt cctcgcgcgc ccgcgcgagg acccatcggt ccatccggac
     2641 tgcgtcagac ccggtgctac actcgctgac cgatgccccc aaagaaacgt gccgcaggcg
     2701 acgtcgtgat cgtcgagtcg cccgccaagg ccaagaccat cgagcgatac ctgggtcccg
     2761 gttacaccgt gctggcctcc tacggccatg tccgtgacct gccccagcgc agcctgggcg
     2821 tcgacgtcga caatgacttc gagccgcgct acgagccgct gaaggaccgc cgccgggagc
     2881 tggagaagct ggccaaggag gcgcgccacg ccgatcacgt gtggctggcc accgacctcg
     2941 atcgcgaggg ggagtcgatc gcctggcaca tcgccgagta cgcggacctg ccggcggacc
     3001 gcatccgacg cgtcaccttc agcgagatca ccaagtcggc catcgacgag gcgttcgcaa
     3061 acccgcgggc catcaaccag gacctggtga acgcccagca ggcgcggcgc gtcatcgacc
     3121 ggctggtggg ctacaagctc agcccgctgg tgagcagcaa gatccgccgc ggcctgtcgg
     3181 cgggccgtgt tcagagcgtg gccgtgcgcc tggtggtcga ccgcgagcgg gagatcgacg
     3241 ccttcacgcc agaggagtac tggagcatcg aggcgcagct tcggcggcgg ggaagcggtg
     3301 ccgggttcaa ggccgagctc acgcgcatcg acggcgagaa ggcaaagatc ggctcggaga
     3361 aggaggcgcg ggcccacgag gcggcgctgc gcgaggcgga gtacgtcgtc agccgcgtca
     3421 cccgcagtca gcagcgcaag aacgcgccgg cacccttcac caccagcacg ctgcagcagg
     3481 aggcctcgcg caagctcggt ttcggcgcca aacggaccat gagcgtggcg cagtcgctgt
     3541 acgagggcat cgcgctgccc gagggccagg tcggcctgat cacctacatg cggaccgatt
     3601 cggtggtgat ggcctccggc gcggtcgccg aggcacgcga ggtgatcgcc cgccgccacg
     3661 gcccggagtt cgtgcccgac aagccgaacg tcttccgtac caaggcgcgg ggagcgcagg
     3721 aggcgcacga ggcgatccgc ccgtcgagct tcgcacggag cccggactcg ctgcgctcca
     3781 gcctcaagcc agacgagctc aagctgtacg ccctgatctg gcggcgcgcc ctggcatccc
     3841 agatggagcc ggcgcgcttc gaccaggtcg gcgtcgacat caccgctgac cgatacctgc
     3901 tgcacgccgg ggcgcggaag cgcatcttcg acggctacca ggcgctctac gtggagggcc
     3961 gcgacgacga ggacgacgag cgcctgaccg tccttcccga cctggccgat ggcgagccgc
     4021 tggacctggc cgacctggcc tccgagcagc acttcaccca gccaccgccg cgctacaccg
     4081 aggcgaccct gatccgcgcc ctcgaggagc acggcatcgg gcgtccgtcg acctacgccc
     4141 ccaccctgga caccattcgc cagcgtggct acatcaccgt caaggagcgg cgcctcttcc
     4201 ccgaggagtc cgccctgcgc gtcaccgacc tgctcaagga gca
//
LOCUS       DATACA010000545         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2245074, whole genome shotgun sequence.
ACCESSION   DATACA010000545 DATACA010000000
VERSION     DATACA010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245074"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VHK28_11025"
     CDS             complement(<1..994)
                     /locus_tag="VHK28_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX2142784.1"
                     /translation="MEVRIAVTDPSDRPATREGRGRNLAFIAFAFLLTVPWLALNLQG
                     AHPEPLAMAALSGLAILGAAFLLSWAAEVFQMEVSQALALALLALIAVLPEYAVDAVF
                     AYQAGQDPVVAQEGYAVANMTGANRLLIGLGWSSIVLLAWFRHGARSVALERSQAVEM
                     AILLVATMYALVIPFKGELNLLDTAILVTLFGLYTWATSRAPAEEPHLVGPAKIIGST
                     GTNTRRVLTLFLFAFAAYAIFVSAHPFAEGLVDTGRAYGVDEFLLVQWLAPLASESPE
                     FIVALLFVWRGQAAAGMRTLVSSKVNQWTLLIATLPVVFSFGAGHLGGMPLVERQ"
     gene            1305..1583
                     /locus_tag="VHK28_11030"
     CDS             1305..1583
                     /locus_tag="VHK28_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX2142785.1"
                     /translation="MSARPTGPADRSIRRLTPSERGLALLVADGLDDRAIARRVGISA
                     NAVRTWVRRIRWRLGLETRADLVAWVRARRAPDAPEGRLRRGKDAQSD"
BASE COUNT          285 a          632 c          646 g          215 t
ORIGIN      
        1 gctggcgctc cacgaggggc atgccaccca ggtgccccgc gccaaaactg aagacgactg
       61 gcaaggtggc gatcagcagc gtccactggt tcaccttgct cgacaccaac gtgcgcatgc
      121 cggcggccgc ctggccgcgc cacacgaaca gcagcgccac gatgaactcg ggagattccg
      181 acgcgagcgg cgccagccac tgcaccagca ggaactcgtc cacgccgtag gcccggccgg
      241 tgtccaccag cccctcggcg aacgggtggg cggagacgaa gatggcgtac gcggcgaacg
      301 cgaagaggaa cagcgtcagg acccgacggg tgttggtgcc ggtcgacccg atgatcttgg
      361 cggggccgac gaggtggggc tcctcggcgg gtgcccgcga ggtggcccag gtgtagaggc
      421 cgaacagcgt caccaggatc gccgtatcga gcaggttcag ctcgcccttg aacgggatga
      481 cgagcgcgta catcgtggcc accagcagga tcgccatctc caccgcctgg ctgcgctcca
      541 gcgcgacgct gcgcgcgccg tggcggaacc aggccaggag gacgatcgag ctccagccga
      601 ggccgatcag cagccggttg gcgccggtca tgttggcgac cgcgtagccc tcctgcgcga
      661 cgaccggatc ctggcccgcc tggtaggcga agacggcgtc gacggcgtac tccggcagca
      721 ccgcgatcag cgccagcagc gccagcgcca gcgcctgcga gacctccatc tggaacacct
      781 ccgccgccca ggagagcagg aaggccgcgc cgaggatggc caggcccgag agcgccgcca
      841 tcgccagcgg ctccggatgg gcgccctgca gattgagcgc aagccagggg acggtcagca
      901 ggaacgcgaa ggcaatgaag gcgaggttgc ggcccctgcc ttcccgggtg gcggggcggt
      961 cactagggtc agtcacggcg atgcggacct ccacgtgagc cgttcgtcga ggcgggcgtg
     1021 gaaacgcgcg gggcgtgtct gcgcggcacc cggatgaacg gcgaagatgc tcgtggaagg
     1081 tctcgctcgg gcccgcgggg acccgccacg gccgggggca gctccgttcg agcggcctcc
     1141 gtgctgacga ccgggatgcc gcgagccctc accaggcccg ggagctactc cccttccgca
     1201 tccacagctt accacgtcca cacggcgtgt tcggcaaccc tgatatcttc ggcgaccagg
     1261 gcatctcccc ccgccatgcc gttgcgttgg aatcggcatg cgacgtgtcc gcgcgaccga
     1321 ccggccccgc ggaccgctcg atccgccgcc tgacgccgag cgaacgcggg ctggccctcc
     1381 tcgtggccga cgggctcgac gaccgggcga tagcccggcg agtcggcatc tcggcgaacg
     1441 cagtccgcac ctgggtcagg cgcatccgct ggcggctcgg cctggagacc cgcgccgacc
     1501 tggtggcctg ggtgcgggcg cgacgcgcgc cggacgctcc cgaggggcgg ctgcggcggg
     1561 gcaaggacgc gcaatccgac tgacgcagcg ctcgcaccgc gatggcgacg cttaccgcgc
     1621 gtcgcggggc gggaggtcga tgagcccccg gtcgatggcg acgaccagcg cctccagctt
     1681 ggacccggcg ccgagcttcg cgatggtcga tgcgagggcg cggcggacca cctcgggcgg
     1741 cgctcccagg aggtccgcga cttcgacgac gccgcgcc
//
LOCUS       DATACA010000546         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1200417, whole genome shotgun sequence.
ACCESSION   DATACA010000546 DATACA010000000
VERSION     DATACA010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200417"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VHK28_11035"
     CDS             complement(<1..716)
                     /locus_tag="VHK28_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX2142786.1"
                     /translation="MATRQRSEPAMVRAARRLLGDRRLLIATNRGPVTFTVGPDGALR
                     PRRGSGGLVTALSQVGHHVPVTWMAAPLSEGDRRAAADPKLADKALPSEEIRLRFASV
                     ERSAHEAAYNVIANPLLWFLQHQMWDLPTRPVIDAGTMRAWENGYVPVNRSFGEALLR
                     EVGNDPEPRIMLHDYHLYLAADLIRQERPRALLSHFTHIPWPPSSLWQNISPTIRSGI
                     VAGLTANDVVGFQTERYAHN"
     gene            complement(721..>1757)
                     /locus_tag="VHK28_11040"
     CDS             complement(721..>1757)
                     /locus_tag="VHK28_11040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2142787.1"
                     /translation="LLTGFHKTDRFSNEGGLKYFLLGSFASAILLFGIAWAYGLTGST
                     RIGDIGPGLLEGGPAGLVAIGMLTVGATFKAAAIPFHYWTPDAYQGAPTPITGFLSVG
                     PKLGAFALLVRIFVDGLGEMRADWLTVMAVLAVLTMTGGNVVALVQTNVKRMLAYSSI
                     AHTGYIMAGLAAYAAATSPELQQEGIEAILFYVLGYGVMNLAAFAVVGMLQRDPRRYG
                     GLASFAGLGRRSPGIAAAMAILLLSLTGIPPTVGFFAKLYVLLASVNAGLAWLAVIIA
                     LNAALAAFYYLRVIVYMYMRDPEDEPAPIDRHPFGAVALTLSVVGVILLGIFPEPILD
                     LLQTSAASIF"
BASE COUNT          290 a          585 c          639 g          243 t
ORIGIN      
        1 aagttgtgcg cgtagcgctc ggtctggaag ccgaccacgt cgttggccgt cagcccggcc
       61 acgatcccgc tgcggatggt gggcgagatg ttctgccaca gcgaggacgg cggccagggg
      121 atgtgggtga agtggctgag cagcgcccgc gggcgctcct ggcggatgag gtcagcggcc
      181 aggtacaggt ggtagtcgtg gagcatgatc cgcggctccg ggtcgttgcc cacctcgcgc
      241 agcagcgcct cgccgaagct gcggttgacc gggacgtagc cgttctccca cgcgcgcatg
      301 gtgccggcgt cgatcaccgg ccgcgtgggc agatcccaca tctgatgctg caggaaccac
      361 agcagcgggt tggcgatcac gttgtaggcc gcctcgtgcg cggatcgctc gacgctggcg
      421 aagcgcagcc tgatctcctc ggagggaagc gccttgtcgg ccagcttagg gtcggcggcc
      481 gcccgccggt ccccctcgct gagcggagcc gccatccagg tcaccgggac gtggtgcccg
      541 acctggctga gcgcggtgac cagcccgccc gatccgcgcc gcggacggag ggcgccatcc
      601 gggccgacgg tgaacgtgac cggcccgcga ttggtggcga tcagcagccg gcggtcaccg
      661 agcaggcggc gggcagcccg caccatggcc ggctcgctgc gctgccgggt ggccatcggt
      721 ctagaagatg ctggctgctg acgtctggag gagatccagg atcggctccg ggaagatccc
      781 gagcaggatg acccccacca ccgacagcgt cagcgccacc gcgccgaagg gatgccggtc
      841 gatcggcgcc ggctcgtcct ccgggtcgcg catgtacatg tagacgatca cgcgcaggta
      901 gtagaaggcg gccagcgccg cgttgagcgc gatgatcacc gccagccagg ccagcccggc
      961 gttcacgctg gccagcagga cgtagagctt ggcgaagaag cccaccgtgg gaggaatccc
     1021 cgtcagcgac aggagcagga tggccatggc cgcggcaatg ccaggagacc tccgaccgag
     1081 gccggcgaac gaggccagcc cgccgtagcg gcgcgggtcg cgctgcagca tgccgactac
     1141 cgcgaaggcc gccaggttca tgacgccgta gcccagcacg tagaacagga tggcctcgat
     1201 gccctcctgc tgcagctccg ggctggtggc tgccgcgtag gccgccaggc cggccatgat
     1261 gtagccggtg tgcgcaatgc tcgagtaggc cagcatgcgc ttcacgttgg tctgcacaag
     1321 ggccaccacg ttgccgccgg tcatggtcag caccgccagg acggccatca ccgtcagcca
     1381 gtccgcgcgc atctcgccca ggccgtcgac gaagatgcgc accagcagcg cgaaggcgcc
     1441 cagcttgggg ccgacggaca ggaagccggt gatgggcgtg ggggcgccct ggtaggcatc
     1501 cggcgtccag tagtggaagg ggatggccgc ggccttgaag gtcgcgccta cggtcagcat
     1561 gccgatcgcc accaggccgg caggtccacc ctccagcagt ccgggaccga tgtccccgat
     1621 ccgggtgctg ccggtcagcc cgtaggccca ggcgatgccg aacagcagga tggcgctggc
     1681 gaacgagccg agcaggaagt acttgagccc gccctcgttg ctgaagcgat cggtcttgtg
     1741 aaacccggtg agcaggt
//
LOCUS       DATACA010000547         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_718993, whole genome shotgun sequence.
ACCESSION   DATACA010000547 DATACA010000000
VERSION     DATACA010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718993"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VHK28_11045"
     CDS             <1..384
                     /locus_tag="VHK28_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX2142788.1"
                     /translation="PLPPWLARANLVVTNRITRPVAGVLPWFGVLEHVGRRSGMVRRT
                     PLNIFPRGNGVWVVALTYGPGVQWLRNVEAAGRCRVLVRGRWLRMVEPCRFRDPARRP
                     VPRVVRPILAVLRVDWFVELREPLE"
     gene            complement(627..1037)
                     /locus_tag="VHK28_11050"
     CDS             complement(627..1037)
                     /locus_tag="VHK28_11050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX2142789.1"
                     /translation="MPLQLEIVSPERRAYDDEVDMVVVPGIDGQLGILPGHTRLLTAL
                     GTGELKIRRAGTETLMLISGGFVEVRPDRVVIMADLAEHSDEIDEAKAVEARKRAEAE
                     LGEVKDPVDIARVRAALQTALMRERIATRRRSRG"
     gene            complement(1063..>2106)
                     /gene="atpD"
                     /locus_tag="VHK28_11055"
     CDS             complement(1063..>2106)
                     /gene="atpD"
                     /locus_tag="VHK28_11055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX2142790.1"
                     /translation="SFEDMETEAQIFETGIKVIDLIAPFKRGGKVGVFGGAGVGKTVT
                     IQELIRNIAQEHGGYSVFAGVGERSREGNDLIGEMTESGVIEKTVMVFGQMNEPPGAR
                     QRVGLTGLTMAEYFRDVEGRDILLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAT
                     EMGDLQERITSTTKGSITSLQAIFVPADDYTDPAPATTFGHLDSTIRLERAIVEIGIY
                     PAVDPLTSTSTVLDPRIVGDEHFAVAREVQRTLQRYKDLQDIIAILGMDELSEEDKLT
                     VGRARRLQRFMSQPMFVAEQFTGRPGKYVQRMETVRGAKEILDGKHDQLPEQAFLMAG
                     PIEDVVEQAEAMK"
BASE COUNT          290 a          712 c          741 g          363 t
ORIGIN      
        1 ccccttcctc cgtggctggc gcgggccaac ctggtggtga ccaaccggat cacgcggccg
       61 gtggccggcg tgctgccgtg gttcggcgtg ctggagcacg tcgggcggcg gagcggcatg
      121 gttcggcgca cgccgctcaa catcttcccg cgggggaatg gggtctgggt cgtggcgctg
      181 acctacgggc cgggcgtgca gtggctgcgc aacgtggagg ctgccggtcg ctgccgcgtg
      241 ctggtgcgcg gccgctggct gcgcatggtc gagccgtgcc ggttccgcga tcctgcacga
      301 cggccggtcc cgcgggtggt gcggccgatc ctggcggtgc ttcgcgtgga ctggttcgtg
      361 gagctgcgcg agcccttgga gtgagctggc tgttcacatc tgcgactggg agtcgcgtta
      421 caggcgatcg gtcgccattt cactctcagg cgcgactggt gatcgcggta gagacccgca
      481 acggccgacc tgttcacagg tgttctccgt cagtcgcagg tgagagcggc accacgggcg
      541 gctgagcgtg gctatgccga caacccgtga gaacagttct ctaacgcgcc tggtgttcgc
      601 gcatgagagt gccccgcagg cccaacctac ccgcgcgagc ggcggcgagt ggcgatcctc
      661 tcccgcatca gcgccgtctg caacgcggcg cggacgcggg cgatgtccac cgggtccttc
      721 acctcgccga gctctgcctc ggcccgcttg cgtgcctcca cggccttggc ctcgtcgatc
      781 tcgtcggagt gctcggccag gtcggccatg atgaccacgc ggtccgggcg cacctccacg
      841 aagccgccgg agatgagcat cagcgtctcg gtgccggcgc ggcggatctt cagctccccg
      901 gtgcccaggg cggtgagcag gcgggtgtgg ccgggcagga tgcccagctg cccgtcgatg
      961 ccgggcacca cgaccatgtc cacctcgtcg tcgtaggcac ggcgctccgg gctgacgatc
     1021 tccagctgca gcggcatcaa cccacctcgc acgcaggcat cgttacttca tcgcctccgc
     1081 ctgctcgacc acgtcctcga tcggtccggc catcaggaac gcctgctcgg gcagctggtc
     1141 gtgcttgccg tccaggatct ccttggcgcc gcgcacggtc tccatgcgct gcacgtactt
     1201 gccggggcga ccggtgaact gctccgcgac gaacatcggc tgggacatga agcgctgcag
     1261 gcgtcgggcg cggcccacgg tcagcttgtc ctcctccgac agctcgtcca tgcccaggat
     1321 ggcgatgatg tcctgcaggt ccttgtagcg ctgcagggtg cgctgcacct cgcgcgcgac
     1381 cgcgaagtgc tcgtcgccca cgatccgagg gtcgagcacg gtcgaggtgg aggtcagcgg
     1441 gtcgaccgcc gggtagatcc cgatctccac gatcgcgcgc tcgaggcgga tggtggagtc
     1501 caggtggccg aaggtggtgg ccggggcggg atcggtgtag tcgtcggccg ggacgaagat
     1561 ggcctgcagg gaggtgatgg atcccttggt ggtcgaggtg atgcgctcct gcaggtcgcc
     1621 catctcggtg gccagcgtcg gctggtagcc caccgcggag ggcatccgac ccagcagggc
     1681 cgagacctcc gagcccgcct gggtgaagcg gaagatgttg tcgatgaaca gcaggatgtc
     1741 gcggccctcg acgtcgcgga agtactcggc catggtcagg ccggtcaggc cgacccgctg
     1801 gcgagcgccg ggcggctcgt tcatctggcc gaagaccatg accgtcttct cgatcacgcc
     1861 cgactcggtc atctcaccga tgaggtcgtt cccctcgcgc gaccgctcgc ccacccccgc
     1921 gaacacggaa tagccgccgt gctcctgggc gatgttgcgg atcagctcct ggatggtgac
     1981 cgtcttgccc acgccggccc cgccgaagac gccgaccttg ccgccgcgct tgaagggcgc
     2041 aatgagatcg atgaccttga tgcccgtctc gaagatctgg gcctcggtct ccatgtcctc
     2101 gaacga
//
LOCUS       DATACA010000548         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1762575, whole genome shotgun sequence.
ACCESSION   DATACA010000548 DATACA010000000
VERSION     DATACA010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762575"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="yihA"
                     /locus_tag="VHK28_11060"
     CDS             <1..605
                     /gene="yihA"
                     /locus_tag="VHK28_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX2142791.1"
                     /translation="GRKLFAGPCDFMLGVAALHQLPEAGPPEVAFAGRSNVGKSSLIN
                     ALRGRSALARTSKTPGRTQQLNLFDLGGGRLILVDMPGYGYAQAPKPLVDSWNRLIRG
                     YLRGRPTLRRVCLLVDARHGVKDSDRALMDMLGEAAVVFQVVLTKSDAIGERQLAERI
                     GDIGAELAKRTAAHPQVLATSAAKGTSLERLRAELAALAA"
     gene            717..1217
                     /locus_tag="VHK28_11065"
     CDS             717..1217
                     /locus_tag="VHK28_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006102057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HEX2142792.1"
                     /translation="MTTGTRADLIDRPQREVTREEDFVAWARGQAALLRRGELETVDR
                     EGVAEELEDMTKRERRELESRLKILLQHLLKWEFQPQRRSRSWLLTIREQRNQLGKLL
                     RDSPSLKNDVAWAIEENYSTAAGDAAVETRMPRGAFPPLCPYPQADVLDPGFLPGPPA
                     DDEKLL"
     gene            1287..>1501
                     /locus_tag="VHK28_11070"
     CDS             1287..>1501
                     /locus_tag="VHK28_11070"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019950683.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX2142793.1"
                     /translation="MTTEAHHHRVAATLIEALPYMRRFSGATFVIKYGGHAMGSAAVA
                     HDFARDVVLLKQVGINPVVVHGGGPQI"
BASE COUNT          230 a          518 c          559 g          194 t
ORIGIN      
        1 ccggccgcaa gctgttcgcc ggcccctgcg acttcatgct cggcgtggcc gcgctccacc
       61 agctgcccga ggcggggccg ccggaggtcg cgttcgcggg gcgctccaac gtcggcaagt
      121 cgagcctgat caacgcgctc agggggcgga gcgccctggc ccggacctcg aagacgcccg
      181 gccggacgca gcagctcaac ctcttcgacc tcggcggcgg ccggctgatc ctggtcgaca
      241 tgcccggcta cggctacgcc caggcgccga agccgctcgt ggactcctgg aaccggctga
      301 tccgcggcta cctgcgcggc cgcccgacgc tgcgccgggt ctgcctcctg gtcgacgccc
      361 gccacggggt gaaggacagc gaccgggcgc tgatggacat gctcggcgag gcggcggtgg
      421 tgttccaggt ggtgctgacc aagagcgacg cgatcgggga gcgccagctc gcggagcgga
      481 tcggggacat cggcgcggag ctcgccaagc gcacggccgc gcacccgcag gtgctggcca
      541 ccagcgcggc caagggcacc agcctggagc ggctgcgggc cgagctggcg gcgctggcgg
      601 cctgagcgga agcgcgcaca cgggcggtgt gcgccgatgc cctgctgact cggcggccga
      661 tcgacgcgat aatcaggccg gacgggggag tccggaccac acggagagag gcggccatga
      721 ccaccggcac gcgggcggac ctgatcgatc ggccccagcg ggaggtcacg cgcgaggagg
      781 acttcgtggc gtgggcccgc ggtcaggcgg ctctgctgcg tcggggcgag ctggagaccg
      841 tcgaccggga gggagtcgcc gaggagctgg aggacatgac gaagcgggag cggcgtgagc
      901 tggagagccg gctcaagatc ctcctgcagc acctcctgaa gtgggagttc cagccgcagc
      961 ggcggtcgcg aagctggctg ctgacgatcc gtgagcagcg caaccagctc gggaagctcc
     1021 tacgagacag tccgagtctg aagaacgacg tggcctgggc gatcgaggag aattactcga
     1081 cagcggccgg cgacgcggcc gtggagacca ggatgccgcg gggtgcgttt cccccgctgt
     1141 gcccataccc tcaggcggac gtcctagacc ccgggttcct accgggtcca ccggccgacg
     1201 atgagaagct gctctgagtc ccggttcctc ttcggcaccg gcaccgcgcg tgtcctcccc
     1261 ccgatcgtgg tcaggcgagt gatggcgtga ccaccgaggc ccaccaccac cgcgtcgcgg
     1321 cgacgctgat tgaggcgctg ccctacatgc gccggttcag cggcgcgacc ttcgtcatca
     1381 agtacggcgg ccacgccatg ggctcggccg ccgtcgccca cgacttcgcc cgcgacgtcg
     1441 tgctgctgaa gcaggtcggg atcaacccgg tcgtggtcca cggcggcggg ccgcagatca
     1501 a
//
LOCUS       DATACA010000549         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2485704, whole genome shotgun sequence.
ACCESSION   DATACA010000549 DATACA010000000
VERSION     DATACA010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485704"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VHK28_11075"
     CDS             complement(<1..575)
                     /locus_tag="VHK28_11075"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2142794.1"
                     /translation="MSIDRGRTPVVAGNWKMNPTTLEEAVRLATAVRDGTAGLTVRTV
                     VCPPFVFLPAVARAVAPGGTEPGGLGIGAQTMHAVDSGAYTGEIAPIMLAGLAQYVII
                     GHSERRQYFNETDTAVAAKVAAAVTHGLVPIMAVGESAAERAAGLTSEVVDRQMRAAI
                     SGLDRIEGSGLVIAYEPVWAIGTGLAATPDDA"
     gene            complement(572..1438)
                     /gene="whiA"
                     /locus_tag="VHK28_11080"
     CDS             complement(572..1438)
                     /gene="whiA"
                     /locus_tag="VHK28_11080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEX2142795.1"
                     /translation="MLVSSELKGELARIHPARACCRRAELAGLLYVDRQPAGISTLDH
                     ATARVAVQLAASLGIAASGPRPASGRSTPRGGRHHLLVGLEPGVTAGWTWTAAPACDR
                     RAFLRGVLLNASVSVAGGPHVELVFRDAARARELVAALAEVGVQAALTTRRGRPVVYL
                     KSGEQIATLLRLVGANRTLLDFEAGRVARDVRSRLNRMLNAEEANLDRTVRAADRQRR
                     AIEHLASTGELERLAEGLREVARERRRQPEADLDTLATSLGLSRSAVNHRLRRLVALA
                     DASRGAEEAVHA"
     gene            complement(1446..2762)
                     /locus_tag="VHK28_11085"
     CDS             complement(1446..2762)
                     /locus_tag="VHK28_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HEX2142796.1"
                     /translation="MRRIRIPRWMYPGMHLKRWLLLVFVGITILALGSAIFLVDFYRR
                     LPDDSIIFVLTGAGLDRPIRAALVGAAGILVTGIGVWGLMRSIVSPFIARGDSVMEVL
                     YTKRYLARGPRIVAIGGGTGLSNLLRGLKGYSANITAIVAVADDGGSSGRLRQQLGIV
                     PPGDIRNCIAALADAEPLMTQLMQYRFPPGSGLDDHAFGNLFIAAMTAVTGDFEEAVR
                     ESNRVLAVRGQVLPATSVPLNLGAVLASGRRLQGQVAISHAEEPIERVTIEPDDVRAN
                     PEALERILEADLVVIGPGSLFSSVLPNLLISDIRDALSAASGVRIYVCNVATQPGETD
                     SFTAAEHLEALFAHVGDDLVDYALVNANTAARRPEGWLGQPVPVDERGLEELPVTIVE
                     EDLVDVANAHRHDSAKLASALMRLHQEERADRIRQRRVRRPTASAV"
     gene            complement(2759..>3471)
                     /gene="rapZ"
                     /locus_tag="VHK28_11090"
     CDS             complement(2759..>3471)
                     /gene="rapZ"
                     /locus_tag="VHK28_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX2142797.1"
                     /translation="RYARVALVLDIRAGDPAPAIEQARAALTGRGVRMEVVFLEAGDA
                     TLVRRFSETRHRHPLQERSGIQAGIGEERRRLAATRELADHVIDTSTLSIGQLKERLF
                     SIVPRQDGTAELRIDVITFGFKYGIPLEADLVFDVRFLTNPFYVPDLKPLSGLQAPVR
                     DYVLGQPAAGRFLQLVQELLELTVPAYRVEGKARLMVALGCTGGYHRSIVLAEELARR
                     LGELEDVDVAVFHRELER"
BASE COUNT          516 a         1241 c         1242 g          472 t
ORIGIN      
        1 gcatcgtctg gcgtggccgc caagccggtg ccgatggccc acaccggctc gtacgcgatc
       61 accagcccgc tgccctcgat ccggtcgagc ccggagatgg cggcacgcat ctgtcggtcc
      121 accacctcgg acgtaagtcc cgcggcgcgc tcggcggcac tttcccccac ggccatgatt
      181 ggcaccaggc cgtgggtgac cgccgcggcg accttggccg ccacggcggt atcggtctcg
      241 ttgaagtact gccgccgttc cgagtggccg atgatcacgt actgcgccag cccggccagc
      301 atgatcggcg cgatctcgcc ggtgtacgcg cccgagtcca cggcgtgcat ggtctgggca
      361 ccgatgccga gacctccggg ctcggtgccc ccgggcgcga ccgcccgcgc gaccgccggc
      421 aggaacacga agggcgggca gaccacggtg cggaccgtca agccagccgt cccgtcgcgc
      481 actgccgtcg caagccgcac cgcctcctcg agcgtggtcg ggttcatctt ccagttgccg
      541 gcgaccacgg gtgtccggcc acgatcgatg ctcatgcgtg cacggcctct tcggcacccc
      601 gggaggcatc ggccaaagcc accagccggc gcagccgatg gttgaccgcg gagcggctca
      661 ggccaaggga cgtggcgagg gtatcgaggt cggcctccgg ctgccggcga cgctccctgg
      721 ccacctcgcg cagcccttcc gccagccgtt ccagctcccc agtcgaggcc aggtgctcga
      781 tggcgcgccg ctggcggtca gctgcgcgca cggtgcgatc aaggttggcc tcctcggcgt
      841 tgagcatccg gttcagtcgg ctgcgcacgt cccgcgccac gcgcccagcc tcgaagtcga
      901 gcagcgtgcg gttggccccc accagtcgca gcagggtcgc gatctgctca ccgcttttga
      961 ggtagaccac cggccggccg cgacgcgtcg tgagcgcggc ctggacgccg acctcggcga
     1021 gcgcggcgac cagctccctg gcgcgggccg cgtcccggaa gacgagctcc acgtgggggc
     1081 cgccggcgac cgagaccgag gcgttcaaca gcacgccgcg caggaaggca cgacgatcgc
     1141 aggcgggggc cgccgtccag gtccagccgg ccgtgacacc cggctcgagg ccgaccagca
     1201 ggtgatgccg accaccccgg ggtgtgctcc ttcccgaggc agggcgcggg ccactggccg
     1261 cgatgcccag cgaggcagcc agctggacgg ccacccgcgc cgtggcgtga tcgagagtgc
     1321 tgatcccggc tggctggcgg tcgacgtaga gcaggccggc cagctcggcg cggcggcagc
     1381 aggcgcgggc ggggtggatt cgggcgagct cccccttcag ctcgctggag accagcatca
     1441 gggcgtcaga ccgcggacgc cgtgggccgt cgcacgcggc gctggcggat gcggtccgcg
     1501 cgctcctcct ggtgcagtcg catgagcgcc gaggccagct tggccgagtc gtggcggtgg
     1561 gcgttggcca cgtccaccag gtcctcctcc acgatggtga ccggcagctc ctccaggccg
     1621 cgttcgtcga ccggcaccgg ctggccgagc cagccctccg ggcggcgggc agcggtgttg
     1681 gcgttgacca gggcgtagtc gaccaggtca tcgcccacgt gggcgaagag cgcctccagg
     1741 tgctcggctg ccgtgaacga gtcggtctcg cccggctggg tcgccacgtt gcagacgtag
     1801 atgcgcacgc cgctggccgc gctgagcgca tcacggatgt cgctgatcag caggttgggc
     1861 aggacgctgg agaacaggct gccaggaccg atgaccacca ggtccgcctc caggatgcgc
     1921 tccagggcct ctgggttggc gcggacgtca tcgggctcga tggtgactcg ctcgatgggc
     1981 tcctccgcgt ggctgatggc cacctgtccc tgcaggcgcc ggccggacgc cagcacggcg
     2041 cccaggttga gcggcacact ggtggccggc aggacctggc cccgtacggc caggacccgg
     2101 ttcgactcgc gcaccgcctc ctcgaagtcg ccggtcaccg ccgtcatggc cgcgatgaac
     2161 aggttgccga aggcgtgatc gtccaggccg gagccgggtg ggaagcggta ctgcatgagc
     2221 tgcgtcatca gcggctcagc gtcggccagg gcggcgatgc agttgcgaat gtcgccgggc
     2281 ggcacgatac ccagctgttg gcgcaaccgc ccggacgagc cgccgtcatc ggcgacggcc
     2341 acgatggcgg tgatgttggc cgaatagccc ttcaagccgc gcagcaggtt cgagaggccg
     2401 gtaccgccgc cgatggccac gatccgcggc ccgcgcgcca ggtagcgctt ggtgtacagc
     2461 acctccatca ccgaatcgcc gcgggcgatg aagggcgaca cgatgctccg catcaggccc
     2521 cacacgccga tgccggtcac caggatgccg gccgcgccga ccagggcggc gcggatcggc
     2581 cggtccagcc cggcgccggt cagcacgaag atgatcgagt cgtcgggaag ccgccggtag
     2641 aaatcgacca ggaagatggc gctgcccagg gccaggatgg tgatccccac gaacaccagc
     2701 agcagccagc gcttgaggtg catgccgggg tacatccagc gcgggatgcg gatgcgcctc
     2761 atcgctccag ctcccggtgg aagacggcca cgtccacgtc ctccagctcg cccaggcggc
     2821 gcgccagctc ctcggccagc acgatcgagc ggtggtagcc gccggtgcag cccaaagcca
     2881 ccatcaaccg cgccttgccc tcaacccggt aggcgggcac ggtcagctcc agcagctcct
     2941 ggacgagctg caggaagcgg ccggcggccg gctgcccgag cacgtagtcg cgcaccggag
     3001 cctgcaggcc ggatagcggc ttgaggtccg gaacgtagaa ggggttggtc aggaagcgca
     3061 cgtcaaacac caggtccgcc tccagcggga tgccgtactt gaagccgaag gtgatgacgt
     3121 cgatgcgcag ctcggccgtc ccgtcctgcc gcggcacgat cgaaaagagg cgctccttga
     3181 gctggccgat gctgagcgtg gaggtatcga tcacgtggtc cgccagctcc cgggtggctg
     3241 ccagtcgccg ccgttcctcg ccgatgccgg cctggatgcc gctgcgctcc tgcagcgggt
     3301 ggcggtggcg ggtctcactg aagcggcgca ccagggtggc gtcgccggcc tccaggaaca
     3361 ccacctccat gcgtacgccg cgcccggtga gcgcggcgcg cgcctgctcg atggccggcg
     3421 caggatctcc ggcccggatg tcgagcacca gcgcgacgcg ggcatagcgc t
//
LOCUS       DATACA010000550         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2806991, whole genome shotgun sequence.
ACCESSION   DATACA010000550 DATACA010000000
VERSION     DATACA010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806991"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VHK28_11095"
     CDS             <1..1210
                     /locus_tag="VHK28_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2142798.1"
                     /translation="VGILTATYFVAELVLSPLFGVLSDRLGAHRVMQWGPVFGAVAVI
                     MTALTTNLWLLGVTRWLEGAAAGASIPSILGYIALATAHDEPLRGRVVSRFEAATLAG
                     IGGGIVAAGWLYDPNALGLGTTAFFLNAGIYVGSLLIYRYGVSELRHGHPPLGSEAEA
                     APPPLDSAELRPGHFNLARYRELLRSPQVWLLAPTWIALNAVLGAWTTQSVFQLVREP
                     SEGFGDQMLMGGFQPWQVSVGLAVAGVIFFAGLFYWGGRFGTLRRTSIIAVGLAGAVV
                     MVLATFALNHSAGWGAPPQVGLAVLAAIGLFVMAGATPAALGLLADISESHPADRGAI
                     MGLYSVFLGLGQIIGALASGTAADWAGIDGLLLASLVLLGIAVLPIHRLRVSEHLVGV
                     PLSSHSAGGD"
     gene            1274..>2423
                     /gene="proS"
                     /locus_tag="VHK28_11100"
     CDS             1274..>2423
                     /gene="proS"
                     /locus_tag="VHK28_11100"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2142799.1"
                     /translation="MRLSKLFFASLRDDPAEAEMVSHRLLVRAGYVRQLGAGIYSLLP
                     LGFRVQQRIEQVIREEMDAIGGQEMEMPVVHPADLWKETGRYQKIGPEMVRFTDRAGR
                     DMVLAMTHEEVVADLTRDLVRSYRQLPAIVYHFQTKFRDEPRSRGGLIRVREFVMKDS
                     YTLDADTESQDRSYQAHFHAYQRIFERLGLRTVAVGADVGMMGGSLAHEFMVFNEHGE
                     DTLVLCEACGYAANQQIAQVRKPGPPSAEPMPTEEVATPGTDTIASLAEHLGVGTDTT
                     AKAAFFVTGDGRLVTAVVRGDFEVNETKLVNALGAVGGLRPAQAEEIRAAGMEPGYGS
                     PVRAHDTTVVVDDLVAASPNLVAGANRNGYHLLNVNVGRDFTPDLVTDI"
BASE COUNT          324 a          839 c          860 g          400 t
ORIGIN      
        1 ggtcggaatc ctgaccgcca cctatttcgt ggccgagctg gtgctctccc ccctcttcgg
       61 cgtgctctcc gaccggttgg gcgcgcaccg cgtcatgcag tggggcccgg tgttcggtgc
      121 ggtcgcggtg atcatgaccg cgctgaccac caacctgtgg ttgctgggcg tgacccgctg
      181 gctggagggc gctgccgccg gggccagcat tccctccatc ctgggctaca tcgcgctggc
      241 caccgcccac gacgagccgc tgcgcggccg cgtggtcagc cgcttcgagg cggcaaccct
      301 ggccggcatc gggggcggga tcgtcgccgc cggctggctg tacgacccga atgccctggg
      361 cctgggcacg actgccttct tcttgaacgc gggcatctac gtcggctcgc tgctgatcta
      421 ccgctacggg gtatcggagc tgcgccacgg acacccgccg ctcggctcgg aggcggaggc
      481 cgcaccgccg ccgctcgact cagccgagct gcgtcccggg cacttcaacc tggcgcgcta
      541 ccgcgagctg ctgcgctcgc cgcaggtctg gctgctggcg cccacctgga ttgcgctgaa
      601 cgcggtgctg ggagcgtgga ccacccaatc ggtcttccag ctggtgcgtg agccatccga
      661 ggggttcgga gaccagatgc tgatgggtgg cttccagccg tggcaggtga gcgtggggct
      721 ggcggtggcg ggcgtgatct tcttcgccgg cctcttctac tggggcggcc gcttcggaac
      781 cctgcgacga acgagcatca tcgccgtggg actggccggg gcggtggtca tggtgctcgc
      841 cacctttgcc ctcaaccact cggccggctg gggcgcgccg ccccaggttg gcctggccgt
      901 gctggccgcc atcggcctgt tcgtgatggc cggtgccacg ccggccgcgc tgggcctgct
      961 ggccgacatc agtgagagcc acccggccga ccgtggcgcg atcatgggcc tctactcggt
     1021 gttcctgggc ctgggccaga tcatcggtgc gctggccagc ggcacggccg ccgactgggc
     1081 cggcatcgac gggctgctgc tggccagcct ggtcctgttg ggcatcgccg tgctgcccat
     1141 ccatcggctt cgcgtttcgg agcacctggt cggcgtgccg ctgagcagtc attcggccgg
     1201 gggcgactga gcggccgtcc cgttcgcccg gccggtggat gggacccatc ggtccgctat
     1261 ccttcacgcc ctcatgcgcc tctccaagct cttcttcgcc agcctgcgcg acgacccggc
     1321 cgaggccgag atggtcagcc accggctcct ggtacgcgcc gggtacgtgc ggcagctggg
     1381 ggccggcatc tactcactgc tgccgctcgg gttccgcgtg cagcagcgca tcgagcaggt
     1441 catccgcgag gagatggatg ccatcggcgg gcaggagatg gagatgccgg tggtacatcc
     1501 cgcggacctg tggaaggaga ccgggcgcta ccagaagatc ggcccagaga tggtgcgctt
     1561 caccgatcgc gccgggcggg acatggtgct ggccatgacc cacgaggagg tggtcgcgga
     1621 cctgactcgc gatctggtgc gctcctaccg gcagctgccg gccatcgtct accacttcca
     1681 gaccaagttc cgcgacgagc cgcgctcgcg cggcggcctg atccgcgtcc gcgagttcgt
     1741 gatgaaggac tcctacacgc tggacgccga caccgagagc caggaccgct cctaccaggc
     1801 gcacttccac gcgtaccagc gcatcttcga gcggctgggg ctgcgcacgg tggccgtcgg
     1861 cgcggacgtg ggcatgatgg gcggcagcct ggcgcacgag ttcatggttt tcaacgagca
     1921 cggcgaggac accctggtgc tgtgcgaggc gtgtggctac gccgccaacc agcagatcgc
     1981 gcaggttcgc aagccggggc ctccttccgc tgaaccgatg ccgaccgagg aggtcgccac
     2041 ccccgggacc gataccatcg cgtcactggc cgagcacctt ggcgtgggga ccgatacgac
     2101 cgccaaggcg gcgttcttcg tgaccggcga cgggcgcctg gtcacggcgg tggtgcgcgg
     2161 cgacttcgag gtcaacgaga ccaagctggt gaacgcgctg ggggcagtcg gtggcctgcg
     2221 gccggcgcag gcggaggaga tccgcgctgc cggcatggag cccggctacg gttcgcctgt
     2281 gcgcgcccac gacaccacgg ttgtggtgga cgacctggtc gccgcctcgc cgaacctggt
     2341 ggccggcgcc aaccgcaacg gctaccacct gctgaacgtg aacgtcggcc gcgacttcac
     2401 gccggacctg gtgaccgata tca
//
LOCUS       DATACA010000551         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_238681, whole genome shotgun sequence.
ACCESSION   DATACA010000551 DATACA010000000
VERSION     DATACA010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238681"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1359)
                     /locus_tag="VHK28_11105"
     CDS             complement(133..1359)
                     /locus_tag="VHK28_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX2142800.1"
                     /translation="MTEERLPLVELLQKAGDGDFLRAVAEAVLQILMEADVEGLIGAG
                     RHERTAERQTYRNGYRDRALDTRLGTLQLRIPKLRTGPSYFPAFLEPRKTSEKALVAV
                     VQEAWIGGVSTRRVDDLAQAMGLSGINKSQVSKLCKDIDERVNAFLDRPLSGEWPYLW
                     LDATYLKQREGGRIVDVAAIIAVAANGEGRREIVGLHIGPSEAETFWATFLKGLLRRG
                     LRGVKLVVSDAHEGLKAAIRRVMGATWQRCRVHWMRNALAYVPKGQQTMVAAALRQAF
                     LQPDQESARRVWRGMADQFRDRWPKLAKLLDESEHDVLAYMGFPAQHRVKLHSTNPLE
                     RLNKEVKRRADVVGIFPSEPSILRLIGAVLLEANDEWQLQHRYMQVEAMAELMPPLIE
                     ANVTPTASTHSPPQAA"
BASE COUNT          236 a          570 c          548 g          266 t
ORIGIN      
        1 cggctgcgga ctacctcgtc acgcgggaca aggacctgct cgcggcgatg actgtgaccg
       61 ggcggccgca cccgcagacg gtcgcgtctg acaaggtggt ggaaattggc tctgggctga
      121 ggtggccagc ggtcatgcgg cttggggcgg gctgtgggtg gacgcggtcg gtgtaacgtt
      181 ggcctcgatc agcggcggca tcaactcggc catggcctcg acctgcatgt agcggtgctg
      241 aagctgccac tcgtcgttcg cttcgagcag cacggcgccg atcaagcgca ggatggatgg
      301 ctcggagggg aagatgccga cgacgtcggc gcggcgcttg acctccttgt tcagccgctc
      361 caagggattc gtgctgtgca gcttgacccg gtgctgcgcg gggaagccca tgtaggccag
      421 gacgtcgtgc tcgctctcgt ccagcagctt ggcgagcttg ggccagcggt cccggaactg
      481 atccgccatc cctcgccaga cccggcgggc gctctcctgg tccggctgca ggaacgcctg
      541 gcggagcgcc gcggccacca tggtctgctg gcccttgggc acgtacgcca aggcgttgcg
      601 catccaatga acccggcacc gctgccaggt ggcgcccatc acgcgccgga tcgccgcctt
      661 caggccctca tgggcgtcgg agacgaccag cttgacgccg cgcaggccgc gccggagcag
      721 gcccttgagg aacgtggccc agaacgtctc cgcctcgctc gggccgatgt gcaggcccac
      781 gatctcgcgc cggccctcgc cgttggcggc aacagcaatt atggcggcca cgtccacgat
      841 ccgcccgcct tcgcgctgct tcaggtaagt cgcatcgagc cacaggtacg gccactcgcc
      901 gctgagcggc cggtcgagga aggcgttcac ccgctcgtcg atgtccttgc acagcttgct
      961 gacctgggac ttgttgatcc cgctcagccc catggcttgg gccaggtcgt ccacgcgccg
     1021 ggtcgagacg ccgccgatcc aagcctcctg gaccacggcg acgagggcct tctccgaggt
     1081 cttccgcggc tccaggaacg ccggaaagta ggacggaccg gtgcgcagct tggggatgcg
     1141 cagctgcagc gtgccgagcc gggtgtccag cgcccggtcg cgatacccgt tgcggtaggt
     1201 ctggcgctcc gccgtgcgtt cgtgccggcc ggccccgatc aggccctcca cgtcggcctc
     1261 catcaggatc tgcagcacgg cctcggccac ggcgcgcagg aagtcgccat caccggcctt
     1321 ctgcaagagc tcgaccagtg gcagtctttc ctcggtcatc ggacctcctt gttggtcggg
     1381 gtgggagcct cacaactcca ccctagaccg ccggtccggt gaccacctcg ccctgcaccc
     1441 tcatgggcgc cgaaattcca ccacctccgc agacgctacc gcgaggaaag gcccctttcg
     1501 catcacgggt cgtcgccggc cggccccgac gcggtaggcc gggtgtcgcg ttccgcgcgg
     1561 gccgcgcgcc gcaattcgtg acccgaaatg aggaggcgct ggcaatgctc ctgcatcgcg
//
LOCUS       DATACA010000552         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1443152, whole genome shotgun sequence.
ACCESSION   DATACA010000552 DATACA010000000
VERSION     DATACA010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443152"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VHK28_11110"
     CDS             <1..645
                     /locus_tag="VHK28_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142801.1"
                     /translation="GENGGWSPDSTRLLYEDSVLTTDTTGEPARIWLLEVATGEATVI
                     GEGNLTQWLADGEHFSYIPVRTGPVVGDTAPIVVQPLAGGEAVQVALAEDYWWSPDGS
                     ALLLQQADGLYLAEPDGSNARHLVEGGSAVWSPDGSRIAYAYDITSEAEPIIGVTDRD
                     GEVIWSGQVATLPTWSPDGTKLAVQVGVVDLSINILDAKTGEVLWQLEGRDPAW"
     gene            671..1591
                     /locus_tag="VHK28_11115"
     CDS             671..1591
                     /locus_tag="VHK28_11115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX2142802.1"
                     /translation="MSADAERMDPLGATFAALTAVLYGTSYVGTGIALASFGPLTVAS
                     LRGLLGLLALGVFLLLPVGIRWRQGLLPRSSWVRLLVLGIIGGPLFLASMNTAVSLSG
                     ATITSFVAGSYAVLAAALAIPLLRERLSLRIGLGLGLALLGTALLAELHARPDELRGI
                     GWGLAAAVAFAVFLVLSRRWVSPYGLDSATVAITTLALTPLGAGAVALVAGERLPVAP
                     EPAAVAAIAWLAIGPGAAAAMLVVGSMRRLSAARASAFLLLNPPVATLGGALLLGERL
                     SPLQLVGALLVLVAIGTATGAWRLLAGLRP"
     gene            1786..>2189
                     /locus_tag="VHK28_11120"
     CDS             1786..>2189
                     /locus_tag="VHK28_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142803.1"
                     /translation="MRQPATGGTVSGRDRARRLVAILLAALLLQPVANADVASAAPGD
                     AAAAAAWLAAHRGGAASDWQLVYERPAVSPVDGSPIWAGKLVGPAGELATIYRSADGT
                     IGGWDLLEAKAEGAAAALSTLEVKADDALAGAV"
BASE COUNT          271 a          774 c          788 g          356 t
ORIGIN      
        1 ggggagaacg gcgggtggtc gcccgactcg acgcggttgc tctacgagga ctcggtgctg
       61 accaccgaca cgaccggtga gccggcccgc atctggttgc tggaggtcgc aaccggcgag
      121 gcgaccgtca tcggcgaggg aaacctgacc caatggctcg cggacgggga gcacttcagc
      181 tacatcccgg tgcgcaccgg tcctgtcgtc ggcgacaccg ccccgatcgt ggtccaaccg
      241 ctcgccggcg gcgaggccgt ccaggtggcc ttggccgagg actactggtg gtcaccggat
      301 ggttccgcgc tgctgttgca gcaggccgac ggcctctacc tggccgagcc cgatggctcc
      361 aacgcgcgtc acctcgtgga ggggggatcc gcggtgtggt cgccggacgg atcgcgcatt
      421 gcctacgcct atgacatcac ctccgaggca gagccaatca tcggcgtgac cgaccgcgat
      481 ggtgaggtga tctggtccgg acaggtcgcc accctcccga cctggtcccc ggacggcacg
      541 aagctcgcgg tccaggtcgg ggtcgtcgac ctctccatca acatcctgga tgccaagacc
      601 ggcgaagtgc tctggcagct ggagggcagg gatccggcct ggtgactggc tgacccctca
      661 tcatggcggc atgagcgctg acgccgagcg gatggatccg cttggcgcaa cctttgcggc
      721 actgaccgcc gtcctgtacg gcacctccta cgtcggcacc ggcatcgcgc tggcgtcgtt
      781 cggccccctg accgtggcgt ccctgcgcgg cctgctcggg ttgctcgcct tgggcgtctt
      841 cctcctccta ccggtcggca tccgctggcg ccagggtttg ctgccaaggt cgtcctgggt
      901 tcggctgctc gtgctgggga tcatcggcgg gccgctcttc ctggcctcca tgaacaccgc
      961 ggtctcgctg tcgggcgcca ccatcacctc cttcgtggcc ggttcgtacg cggttctcgc
     1021 cgcggcgctg gccatcccgc tgctccggga acggctctcc ctgcgaatcg gactcggcct
     1081 gggtctcgcg ctgctcggca ccgcactcct cgccgagctg cacgcgcggc cggacgagct
     1141 gcgcggcatc ggctgggggc tggccgctgc ggtcgccttc gccgtcttcc tcgtgctgtc
     1201 gcggcgatgg gtctcaccgt acggactgga cagcgccaca gtggccatca ccaccctcgc
     1261 cctcacgccg ttgggagcag gcgccgtcgc cctcgtggcc ggcgagcggt tgcctgtcgc
     1321 gccggagccg gccgccgtgg ctgccatcgc ctggctggcg atcggccccg gcgccgcagc
     1381 cgccatgctc gtggtcggca gcatgcgacg cctgtcggcg gcgcgggcat ccgccttcct
     1441 gctcctcaac ccgccggtgg ccaccctggg cggcgccctg ctgctgggcg aacgactctc
     1501 gccgttgcag ctggtcggtg cgctgctcgt gctggtcgcg atcggcaccg ccaccggggc
     1561 ctggaggctg ctggccggcc tgcgaccctg agcccgcccg gcagccggct tgcaggccgc
     1621 gcgcctcggc cggggacagg acgaatgggc taccgatagg ggatggaccg cggccgccga
     1681 ttggcatgaa ctggcggagc ggcacgctga ggcatccgac ggcggcggtg gcggagtact
     1741 acattgtcat acgagtacat ggtgagggag gcggtcgggc aggtcatgcg tcagcccgca
     1801 accgggggga cagtgtcggg gcgcgatcgt gccaggcggc tggtcgccat cctgctcgcc
     1861 gctctgctgc tgcagccggt ggccaacgcc gacgtggcca gcgcggctcc cggagatgct
     1921 gccgcagccg ccgcctggct ggccgcccat cgcggcggtg ccgcatctga ctggcagctg
     1981 gtctacgagc gtccggccgt ctcccccgtg gacgggagcc caatatgggc tggcaagctg
     2041 gttggtcccg ccggggagct cgcgacgatc taccgttcgg cggacggcac catcggtggc
     2101 tgggacctgc tcgaggccaa ggcggaggga gcggcggcgg cgctcagcac gctggaggtg
     2161 aaggccgacg acgcactggc cggagccgt
//
LOCUS       DATACA010000553         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2407036, whole genome shotgun sequence.
ACCESSION   DATACA010000553 DATACA010000000
VERSION     DATACA010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407036"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..475
                     /locus_tag="VHK28_11125"
     CDS             26..475
                     /locus_tag="VHK28_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX2142804.1"
                     /translation="MRAAADAGLSISVQRFGVETRTASDAARAVGCEVGQIVKSLVFI
                     ADGAAVVALLSGADRLDTDRLAAHLGAADVRRATGDEARQATGYAIGGVPPIGHTNRL
                     PVLMDEGLLEHEVVWAAAGLSDAVFSAEPRALASAAGASIATLAERG"
     gene            complement(487..1044)
                     /locus_tag="VHK28_11130"
     CDS             complement(487..1044)
                     /locus_tag="VHK28_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142805.1"
                     /translation="MNDWRGRSPGRLRTTTTTEVHMDPSSVTRPGTPRRAGWAAARLA
                     VGIVALAACSPSPDSPSPSPSAGLVSDSPRPDASEAVSPSAEPSAGVPGELPAGFPVP
                     PGAGSLAVQPGERIIARWQLNDHGSAAYDHFLRELPAAGFTVEAVAPGGAAASIRFTT
                     PDGEKLQIDLVGTQPVEVELGPPHE"
     gene            complement(1041..>1863)
                     /locus_tag="VHK28_11135"
     CDS             complement(1041..>1863)
                     /locus_tag="VHK28_11135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX2142806.1"
                     /translation="AGKVWWDSVPHWGRVYDNHTGLDYPMALRSTIAAAKAGTVIDVE
                     GGFGTQQKGQFGNFVLMSHSDGRRTLYYHLASAAEGGIHHGVGKQLAAGASVGLSGCS
                     GICGGPHLHFELLLVSGQQEVPTDPMAHRMWTTWPGRVPFLAAYVRENNAGTEVVRRG
                     QTISHWVEFRNTGGRTWRPDIFPGRVVLGTWNPPAHASPFRAADWPLSWMATKVDQAS
                     VPPNSVGRFTFGIRGGPAPGSYVETFNLVAQSVRWFDHARLGGFYVPIVVSNFSP"
BASE COUNT          269 a          632 c          699 g          263 t
ORIGIN      
        1 acctcacccg aacgtcgagc gcgtgatgcg cgccgcggcg gatgccggcc tgtcgatcag
       61 cgtccagcgc ttcggcgtcg agacgcgcac ggcgtccgat gctgcgcgcg ccgtcggctg
      121 cgaggtgggg cagatcgtga agtccctcgt cttcattgcc gatggggcag ccgtggtcgc
      181 cctgctgtcc ggcgcggatc ggctggacac ggatcgactg gcggcgcatc tgggcgccgc
      241 cgacgtgcgg cgggctaccg gtgacgaagc gcggcaggcc acaggctacg caatcggcgg
      301 ggtcccgccc atcgggcata cgaatcgcct gccggtgctc atggacgagg ggcttctgga
      361 gcacgaagtc gtgtgggccg cggccggcct gtcggatgct gtcttcagcg cggagccgcg
      421 ggcgctggca agcgcggccg gggcatccat cgccacgctg gcggagcgcg gctgagcaaa
      481 ttgccgtcac tcgtgtggcg ggccgagctc gacctcgacc ggctgcgtcc cgaccaggtc
      541 gatctgcagc ttctcgccgt cgggggtggt gaaccggatg ctggcagccg cccctccggg
      601 agctactgcc tcgacggtga atccggccgc cggcagctcg cgcaggaagt ggtcgtaggc
      661 ggcgctgcca tggtcgttca gctgccagcg ggcgatgatc cgctctcccg gctgaaccgc
      721 gagtgacccc gcgccgggcg gcacggggaa gccggcgggc agctccccgg gcaccccggc
      781 cgagggttct gccgagggcg ataccgcctc gctggcatcc ggccgagggc tgtcggacac
      841 caggccggcc gagggcgagg gcgagggcga gtcgggagag gggctgcagg cggcaagggc
      901 gacaataccc accgccagcc gggctgctgc ccagccggct cggcgcgggg tcccggggcg
      961 ggtgacggag cttggatcca tgtgcacctc cgtggtggtg gtggtgcgga ggcgtccggg
     1021 cgaacgcccc cgccagtcgt tcacgggctg aagttgctga ccacgatcgg cacgtagaac
     1081 ccacccaggc gggcgtggtc aaaccagcgg accgattggg cgaccaggtt gaacgtctcg
     1141 acatagctcc cgggcgccgg gccgccgcgg atgccgaacg tgaagcgacc gaccgagttg
     1201 ggcgggaccg atgcctggtc gaccttggtc gccatccagc tcagcggcca gtcggcggca
     1261 cggaagggag aggcgtgagc cggcgggttc cacgtcccga gcaccacacg accggggaag
     1321 atgtccgggc gccaggtgcg gccgccggtg ttgcgaaact cgacccagtg gctgatcgtc
     1381 tgcccgcggc gcacgacctc ggtgccggcg ttgttctcgc gcacataggc ggccaggaag
     1441 ggcacgcgcc ccggccaggt ggtccacatg cggtgggcca tgggatcggt gggaacctcc
     1501 tgctggccgc tgaccagcag cagctcgaaa tgaaggtgtg gcccgccgca gatgccgctg
     1561 cacccggaca gccccacgct ggcaccggcc gccagctgct tgccgacgcc atggtggatg
     1621 ccgccctcgg cagcgctggc aaggtggtag tagagggtcc gccgcccgtc agaatggctc
     1681 atcagcacga agttgccgaa ctggcccttc tgctgcgtgc cgaacccgcc ctccacatcg
     1741 atcaccgtgc ccgccttggc ggcggcgatg gtcgagcgca gggccatggg gtagtccagg
     1801 ccagtgtggt tgtcgtacac ccgtccccag tgcgggaccg agtcccacca caccttgccg
     1861 gcc
//
LOCUS       DATACA010000554         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1523558, whole genome shotgun sequence.
ACCESSION   DATACA010000554 DATACA010000000
VERSION     DATACA010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523558"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VHK28_11140"
     CDS             complement(<1..363)
                     /locus_tag="VHK28_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX2142807.1"
                     /translation="MTDEEATMTLMEHLEELRRRLIIIVISVLGAAVFGFVVSRSVLA
                     LLRAPLPDAYDTLFFTSPAGAFAAQLKIAGFVGIAIAMPIILFHVWRFVTPGLTPGER
                     RFVWPVILGGLLLFALGLT"
     gene            complement(492..686)
                     /gene="tatA"
                     /locus_tag="VHK28_11145"
     CDS             complement(492..686)
                     /gene="tatA"
                     /locus_tag="VHK28_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2142808.1"
                     /translation="MPTIGWPELVILLVVVLIVFGPGKLPDIGNAIGRGVKEFRKASN
                     DLEESIRGEPKKPSDRPEAK"
     gene            complement(737..>1627)
                     /gene="pyrF"
                     /locus_tag="VHK28_11150"
     CDS             complement(737..>1627)
                     /gene="pyrF"
                     /locus_tag="VHK28_11150"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX2142809.1"
                     /translation="VGSRRRAGQQPGTGDHMTAVAMTPLERLRERVERLGPLCLGVDP
                     NPDALPEHLTRDVHGIETFARGLLEAAAPSAAAIKINVAFFEAFGSAGWAALERVRAD
                     VPPDHVLILDAKRGDIGSTAERYAEALFGRLAADAVTVSPYLGEDAILPFLAHPDRMV
                     YVLARTSNPSAGTLQDLPVRTGAEELPLHHAVARWVAERWPEARVGLVVGATAPGELR
                     ALRRIVPEPGFLVPGIGPQGGDPATAAKACHGTRAPGLVNVTRGISGAATGSDWRTAA
                     AGEAERWRGLLSGSGATLSS"
BASE COUNT          246 a          563 c          604 g          214 t
ORIGIN      
        1 ggtcaggccc agcgcgaaca gcagcaggcc gcccaggatc accggccaca cgaagcggcg
       61 ctcgccgggg gtcaagcccg gggtcacgaa gcgccagacg tggaacagga tgatgggcat
      121 ggcgatggcg atgcccacga agccggcgat cttgagctgg gccgcgaagg cgcccgcggg
      181 gctggtgaag aagagcgtgt cgtacgcgtc ggggagcggg gcgcgcagca gggccaggac
      241 cgatcgagag acgacgaagc caaacaccgc ggcgcccagg acggagatga cgatgatgat
      301 cagccggcga cggagctcct cgaggtgctc catcagggtc atggtcgcct cttcgtcggt
      361 caccgttgct tcactcgctc agggacgtgg accgcgaacg cgcaccgatc gcgggacacg
      421 ctggctcgat tggcgccacg ctcgggtgtc agccggcccg cgtggtgggc gagtggccgg
      481 accggaatcg gctacttggc ctccggcctg tccgacggct tcttgggctc gccgcgaatc
      541 gactcctcga gatcgttgga ggccttgcgg aactccttga ccccgcgccc gatcgcgttg
      601 ccgatgtccg gcagcttgcc cgggccgaag acgatcagga cgaccacgag caggatgacc
      661 agctcaggcc agccgatggt gggcatgtcg tgcgcaaacc tcgtgtgtcg cgccgggtgg
      721 cgctgggtga tcggggctag gacgaaagtg tagcacccga tccgctgagc aggccccgcc
      781 agcgctccgc ctcgccagcc gccgctgtcc gccagtcaga ccctgtcgcg gctcccgaga
      841 tcccgcgggt gacgttgacc aggcccggcg cccgcgtccc gtggcatgcc ttggcggcag
      901 tcgccggatc cccaccctgc gggccgatgc cgggcaccag gaagcctggc tcgggcacga
      961 tgcggcgcag ggcgcgcagc tcgccaggcg ccgtggcgcc gacaaccagg ccgacccggg
     1021 cctccggcca gcgctcggcg acccagcggg cgaccgcgtg atgcagcgga agctcctccg
     1081 ctccggtgcg cacgggcagg tcctggagcg tgccggcgct cgggttcgaa gtgcgggcca
     1141 ggacgtagac catccgatcc gggtgggcga ggaacggcag gatggcgtcc tccccgaggt
     1201 acggggagac ggtgaccgcg tcggccgcca gccgaccgaa cagcgcctcc gcgtaccgct
     1261 ccgccgtgga gccgatgtct ccccgcttgg cgtccaggat caggacgtgg tccggcggca
     1321 cgtccgcgcg gacccgctcc agtgccgccc acccggcgct gccgaatgcc tcgaagaagg
     1381 cgacgttgat cttgatcgcg gcggcgctgg gcgcggccgc ttcgagcagc ccgcgggcga
     1441 aggtttcgat gccgtgcacg tcacgggtca ggtgctccgg gagcgcgtcg gggttagggt
     1501 ccacgcccag gcacaggggg cccagccgct ccacgcgttc ccgcagccgc tcgaggggag
     1561 tcatggccac ggcggtcatg tggtcgccgg taccgggctg ctgaccggca cggcgacggc
     1621 tcccgac
//
LOCUS       DATACA010000555         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2327386, whole genome shotgun sequence.
ACCESSION   DATACA010000555 DATACA010000000
VERSION     DATACA010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327386"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..432)
                     /locus_tag="VHK28_11155"
     CDS             complement(10..432)
                     /locus_tag="VHK28_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142810.1"
                     /translation="MAHLPPKGGSGNRGYLDGIGALLLGAGALALLFPLVQAESGGLA
                     RLWWLFPIAAAVLATFVAWERRVVGRGAQPVFDPRLVTEVRGFAAGAAVGTAYFVGFG
                     GIWVLFAVFFNRSRLHPTAADAEPSAKGMTDRPDCSGV"
     gene            complement(414..542)
                     /locus_tag="VHK28_11160"
                     /pseudo
     CDS             complement(414..542)
                     /locus_tag="VHK28_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1298..>1959
                     /locus_tag="VHK28_11165"
     CDS             1298..>1959
                     /locus_tag="VHK28_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX2142811.1"
                     /translation="MVGVGMTEPVLGLPMTGLAVTLLRRRLRASDVGEVLAGGALGGA
                     VAMTGSRLFPSRTPSPVRTVRPRPAPQPPRPEGAGVVIVVNPRSGSGRGGRLGEAVRS
                     ALPAADIVTLQPDADVPSAFREAARRAEVLGVCGGDGTVGAAAQVAIEAALPLLVLPG
                     GTFNHFAADLGIDRMADALAALRRGTAVRIDVGLAGERLFLNTSSLGSYPAFVAIRER
                     WEG"
BASE COUNT          263 a          711 c          706 g          279 t
ORIGIN      
        1 cggtctcggc tacaccccgc tgcagtcggg cctgtccgtc atgcccttcg cactcggctc
       61 ggcatcggcg gcagtggggt gtagccgaga ccgattgaag aagacagcga acagcaccca
      121 gatcccgccg aagccgacga agtacgccgt ccccactgct gctccggccg cgaagccacg
      181 aacctcggtg accagccggg ggtcgaacac cggttgggca cccctaccga cgacccggcg
      241 ctcccaggcg acgaaggtgg cgagcacggc cgcggcaatg gggaagagcc accacagcct
      301 ggccaggccg ccggactccg cctgtacgag cgggaacagc agtgcgagcg cgcccgctcc
      361 cagcagcagc gcgccgatgc cgtccaggta cccgcggttc ccggaccctc ccttcggcgg
      421 aagatgcgcc accacgcctc ctgcaggttc agccagcaga cacccttggg gatgaacacc
      481 tgctccaggc gcggatgccc ggccagccac gcgcgggtgg ccaggctgtg atgactggag
      541 aggggacacg gacatctgtc ctcgcacgtc gcagccaccc gccctgacca ccatgtggcg
      601 ccgtccaggt cgtccggcgg ttgcaccttc ttcgtcgcgt gcggggctcg gttgcggacc
      661 gaggtccggc tgctccgcgc gcatggcgtg cagcagccac ttctcgccgg ggcctacggc
      721 aggggagcct gcgtacgaca gttccgctga cgtggtcgac cacccgggac cagggaggag
      781 ccgcccaggt catccggcga atcgcctctg acggggccac ttgcccgtct gaccgcacat
      841 gcaggcaggc ccgagaggac aggagcgtgg tcagggacga tgcgacgagt cgagtggacg
      901 gagagccgcc tgatgagcgc agccggaccg accagggccg ctcccccagc tgggtacagc
      961 agtgacatcc cgttgggatc cgtgcgcact ccgctggcgt cactgtgaga ccggttcgga
     1021 aagcagcgcg gcaggtccgt gcgccggtcc tccacggccg gcttgggccg tcgactccat
     1081 ggtggctggt ctcggccttg ctggcggcca caggtaggcg ccggggccgc cgagcggcga
     1141 tgcgcggcgt ggcctcggtg gtcgccacgg acgcgttgct ccggcggtcc ggctcggacg
     1201 ccgagcgccg gccacgccgg tccggtatgg cactgctctc gcactccgag accgtctcgg
     1261 ggccccgtcc cggcatgctg ccgcggctgc cgccttcgtg gtcggggtcg ggatgaccga
     1321 gccggtgctc gggctgccga tgaccggcct cgcggtgacc ctcctgcggc gccggctgcg
     1381 tgccagcgac gtgggcgagg ttctcgcggg tggcgcactg ggcggcgcgg tagccatgac
     1441 cggcagccga ctcttcccct cccggacacc cagccccgtc cgcacggtcc ggcctcgccc
     1501 ggcgccgcag ccgccccgtc cggagggcgc tggtgtcgtc atcgtcgtga acccacgttc
     1561 cggcagcggc cgtggcggcc ggctgggcga ggccgtgcgc agtgcgctgc ctgctgcaga
     1621 catcgtgacg ctgcagccgg atgccgacgt gcccagcgcg ttccgtgagg ccgctcgacg
     1681 cgccgaggtg ctcggtgtct gcggcgggga cggcaccgtg ggcgccgcgg cgcaggtcgc
     1741 catcgaggcg gccctgcccc ttctggtgct tcccggcggc acgttcaacc acttcgccgc
     1801 cgatctcggg atcgaccgga tggctgacgc gctggcagcg ctgcgccgcg gcacggcggt
     1861 ccggatcgac gtcggcctgg ctggtgagcg gctgttcctc aacacgtcca gcttgggcag
     1921 ctatcccgcg ttcgttgcca tccgggaacg ctgggaggg
//
LOCUS       DATACA010000556         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1363265, whole genome shotgun sequence.
ACCESSION   DATACA010000556 DATACA010000000
VERSION     DATACA010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363265"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="polX"
                     /locus_tag="VHK28_11170"
     CDS             <1..1560
                     /gene="polX"
                     /locus_tag="VHK28_11170"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX2142812.1"
                     /translation="GTPPRIPGAGPALTTKLAELAETGRLEYHERLRAQVPAGLLEIL
                     RVPGVGPRTVRQIHSELGIDSVDALRAAASDGQLRGLKGLSSRTEENVLAGIARMERT
                     SSRLVVHEADRLVAGLLDRLRNVPGVVRIEPAGSLRRRRATIGDLDLLAATTDPAALI
                     AALDGLTDVDRILSAGTDKSSIVLAAGPQVDLMVCPPEAWGTHLVHFTGSKEHNVRLR
                     GMALDRGLSLSEKGFKVIDTGELLTESTEEAVYGRLDLPWIPPELREDGGEIQAGLRG
                     DLPELVALEDVRGDTHVHSDWTDGVDSIEVMARAAQAAGREWMVLTDHSPSLGITRGL
                     VPERVEAQRAEIARLNDELAPFRILHGTEMEIRADATLDYPDELLARFDLVVASVHTA
                     RAQSGEQLTRRTVAAIRNPHVDVIAHPTGRILNRRDPVALDWAEVFRAAAETGTALEI
                     NGSPRLDLDETLARAAAEAGVRLTVASDAHRTEELDQLAYAVSVARRAWIGPQQVLGT
                     RSADAVRGVAK"
     gene            1590..2333
                     /locus_tag="VHK28_11175"
     CDS             1590..2333
                     /locus_tag="VHK28_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142813.1"
                     /translation="MRIRSRVEHPEAILLAVCLAAAAAPGWLGSVFWLAVAIGLQLAV
                     GGLGAVAVLGPVQPGLGFARYAIPAVTGVAVTLLGRQLADVAGLAMLPVAAFVLWAVL
                     RLELAATKSGRAGFLLDLALVAVVFAGAAGIGALLPRDAWPPAVLLVALLAAVPSLRS
                     AEARGRSGVEAVGQGVLHLAAVAQVGTALALLHLQGVVAAAIVALAFHAWGGAAESLD
                     HGASGRAVAVEFGSLAVLGVVVALLLHAG"
BASE COUNT          313 a          870 c          942 g          381 t
ORIGIN      
        1 ggcacgccgc cacggatccc gggcgctggc ccggccctga ccaccaagct ggcggagctg
       61 gccgaaacgg gcaggctgga ataccacgag cggctgagag cgcaggttcc cgccgggctg
      121 ctggagatac tgcgcgttcc cggcgtcggg ccgcgcaccg tgcgccagat ccactccgag
      181 ctgggcatcg actctgtgga tgcgctgcgc gccgccgcgt ccgacggaca gctgcgaggg
      241 ctgaagggcc tctccagccg caccgaggag aacgttctgg ccggcatcgc gcgcatggag
      301 cggacgagca gccgtctcgt cgtgcacgag gcggatcgtc tggtcgccgg gctgctcgac
      361 cggctgcgca acgtgcccgg cgtggtgcgc atcgagccgg ccgggtccct ccgccggcgg
      421 cgggcgacca tcggtgacct tgatctgctg gccgccacca ccgacccggc agccctgatc
      481 gccgcgctcg acgggctgac cgacgtggac cgcatcctgt cggccggcac cgacaagtcg
      541 agcatcgtcc tggcggccgg tccgcaggtc gacctcatgg tctgtcctcc cgaggcgtgg
      601 ggcacgcatc tggtgcactt caccggcagc aaggagcaca acgtccggct gcgcggcatg
      661 gcactggacc gaggcctttc cctctcggag aagggattca aggtgatcga caccggcgag
      721 ctgctgaccg agtccaccga ggaggctgtg tacgggcgcc tggacctgcc ctggatcccg
      781 ccggagctgc gcgaggacgg tggcgagatc caggcggggc tgcggggcga ccttcccgag
      841 ctggtcgccc tggaggacgt gcgcggcgac acgcacgtgc actccgattg gaccgatggc
      901 gtggactcca tcgaggtaat ggctcgcgcg gcccaggcgg ccggccgtga gtggatggtg
      961 ctgaccgacc actcgccctc gctggggatc acccgcggcc tggtgcccga gcgggtggag
     1021 gcgcagcgcg cggagatcgc gcggctcaac gacgagctgg ctccgtttcg catcctgcac
     1081 ggcaccgaga tggagattcg cgccgacgca acgctcgatt acccggacga gctgctggcc
     1141 cgattcgacc tggtcgtggc atccgtgcat acggcacgcg cgcaatccgg cgagcagctc
     1201 acccgccgca cggtggccgc cattcgcaac ccgcacgtgg acgtcatcgc ccacccgacc
     1261 gggcgcatcc tgaatcgccg ggacccggtc gcgctcgact gggcggaggt ctttcgcgcc
     1321 gcggccgaga ctggcacggc gctggagatc aacggcagcc cccggctgga tctggacgag
     1381 accctggcac gggcggcggc ggaggccggc gtgcgcctga cggtggccag tgatgcccat
     1441 cgaaccgagg agctggacca gctcgcgtac gccgtctcgg tcgcccgtcg cgcctggata
     1501 ggtccccagc aggtgctcgg gacgcggtcg gccgacgcag tccggggcgt ggccaaatga
     1561 tcgggcgcat cgtccggcgt gacgctgccg tgcgcattcg ctcccgggtc gagcatccgg
     1621 aggccatcct gctggcggtg tgcctggcgg cggcggccgc acccggctgg ttgggatcgg
     1681 tcttctggct ggcggtggcc atcggcctgc agttggcggt cggaggactg ggcgcagtgg
     1741 ccgtcctggg tcccgtgcag cccggtctcg gcttcgcgcg gtacgccatt ccggcggtga
     1801 ccggggtggc ggtcacgctg ctcgggcgcc agctggccga cgtcgcgggc ctggccatgc
     1861 tgccggttgc cgcattcgtg ctgtgggcgg tgctccgcct cgagctggcc gcgaccaagt
     1921 cagggcgcgc cgggttcctg ctcgacctgg cgctggtggc cgtggtgttc gcgggcgccg
     1981 ccgggatcgg cgccctgctt ccacgcgatg cctggccccc ggccgtcctg ctcgtggccc
     2041 tgctggccgc ggtgccctcc ctgcgctcgg ccgaggcgcg gggccgcagc ggcgtggagg
     2101 cggtcgggca gggtgtgctg cacctggcgg ccgtcgcgca ggtcggcacg gcgctggctc
     2161 tgctgcacct gcagggcgtg gtggccgcgg ccatcgttgc gctggccttt cacgcctggg
     2221 gcggtgcggc cgagtccctc gaccacgggg catcggggcg cgcggtggcc gtggagttcg
     2281 gatcgctcgc agtcctgggc gtggttgtgg cactgctcct gcacgcgggc tgacgctggg
     2341 cggcgctcgt tgacaccccc atcggccggt gctacaacaa ggcgcccttc gcgaccgaag
     2401 cccgttcgcg atgggtttcc ttcctggaat ttgatgtccg caacgcggtt gcggggagac
     2461 ggacccacca tgacactggg tgagacggag acgaccatcc ggcagc
//
LOCUS       DATACA010000557         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2328209, whole genome shotgun sequence.
ACCESSION   DATACA010000557 DATACA010000000
VERSION     DATACA010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328209"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..286)
                     /locus_tag="VHK28_11180"
     CDS             complement(65..286)
                     /locus_tag="VHK28_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX2142814.1"
                     /translation="MCRSIKTLRVLDVPATEDDVRAAALQYVRKVSGYRKPSPANEAA
                     FNAAVEEVTTASRRLLDAVTATHPARRSA"
     gene            complement(296..955)
                     /locus_tag="VHK28_11185"
     CDS             complement(296..955)
                     /locus_tag="VHK28_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX2142815.1"
                     /translation="MTGVGELLPDRYRAVVFDMDGLLLDTEILWHQAETDLFARHGVE
                     FTWDDKMTVIGTSFAFTADYFADRLGVPREQGPALVTEMLELMHDNLRAQVNGRPGAI
                     ELVERLRGRTRLGLASNSPRALVDTALATAGLTDAFEAIVTSDDVDRSKPAPDLYLLA
                     CERLGVMPAEALALEDSASGVAAAKAAGLTCIAVPQFAETDVSAADRVIDSLEDLLAE
                     T"
     gene            complement(952..2169)
                     /locus_tag="VHK28_11190"
     CDS             complement(952..2169)
                     /locus_tag="VHK28_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX2142816.1"
                     /translation="MAADEMRLGGIERIGVLGAGTMGAGIVQVAAEARLTVLVHDPIP
                     GANERAIERIRLHLTRRVEKDELTEQELSEALSCIQPAGSIQDVADADLVVEAIPEDL
                     ELKRDAFRLLDAAADPATILASNTSSLSIARIAAAAQHPQRVIGMHFFNPVPLMALVE
                     VVPGARTDASVTDQVALLTRRLGKTPVVAADTPGFIVNRVARPFYLEALRILGEGTAD
                     IEQVDAAMRGIGFRMGPFELVDVIGLDVNFAVSQSVFEGFFGDPRYRPHPIQRMLVDA
                     GRLGRKTGGGFYDWTDGSRGQAWPGLARRAQGPALPRLGEEQIAARILAAIVNEAASA
                     VAEGVAMPSAIDTAMRLGTNYPSGPLEWGERIGLAHVVHTLDALHAAVPDGRYRVVPL
                     LRSLAERGGSFFA"
     gene            complement(2159..>2517)
                     /locus_tag="VHK28_11195"
     CDS             complement(2159..>2517)
                     /locus_tag="VHK28_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2142817.1"
                     /translation="SGSTRTLVRALGRHRAAALIFSGEPLSAAAAAEVGLVNGVVPAS
                     ELARTARDAAAALAAGPTRALAYAKRLINAAEDAGLDESLALEADLQELAGRTQDHAE
                     GVAAFAEKREPRFVGR"
BASE COUNT          357 a          898 c          915 g          347 t
ORIGIN      
        1 ctggacggag tgcgggtgcg ctgttcgcgt cacgattccg cgtgaaccgc gcgcgagtcg
       61 ccgctcacgc cgagcggcga gcgggatgcg tggcggtgac ggcgtcgagc aggcggcgcg
      121 aggcggtcgt cacctcctcg accgcggcgt tgaaggcggc ctcgttcgcc ggcgagggct
      181 tgcggtagcc gctcaccttg cggacgtact ggagcgcggc ggcgcgcacg tcatcctccg
      241 tcgccggcac gtcgaggacg cggagggtct tgatcgatcg gcacatgccg acagtctagg
      301 tctcggcgag caggtcctcc agcgagtcga tcacgcggtc ggcggccgac acatcggtct
      361 cggcgaactg cgggaccgcg atgcaggtca gcccagccgc cttggcggcg gcgaccccgc
      421 tggcggagtc ctccagcgca agggcctcgg ccggcatcac gcccagccgc tcacaggcga
      481 gcaggtagag gtccggcgcg ggcttggaac gatccacgtc gtcggaggtg acgatggcct
      541 cgaaggcatc ggtcaggccg gcggtggcca gtgccgtgtc gaccagggcg cgcggggagt
      601 tggaggccag ccccagccgc gtgcgcccgc gcagccgctc gaccagctcg atcgccccgg
      661 gacggccatt cacctgcgcg cgcaggttgt cgtgcatgag ctcgagcatc tcggtcacca
      721 gcgcgggacc ttgctcgcgg ggcactccca ggcggtcggc gaagtagtcg gcggtgaaag
      781 cgaagctggt gccgatcacc gtcatcttgt cgtcccaggt gaactccacg ccatgccgcg
      841 caaagaggtc ggtctccgcc tggtgccaca ggatctcggt gtccagcagc aggccgtcca
      901 tgtcgaagac gaccgcccga taccggtcgg gcagcagctc cccgactccc gtcacgcgaa
      961 gaaggacccg ccgcgctcgg ccagggagcg aaggaggggc acgacccggt accgaccgtc
     1021 gggcacggcg gcgtgcagcg cgtccagggt gtgcaccacg tgcgccaggc cgatccgctc
     1081 gccccactcc agcggccccg aggggtagtt ggtccccagc cgcatggcgg tgtcgatggc
     1141 gctgggcatg gcgaccccct cggcaaccgc ggacgccgcc tcgttcacga tggcggccag
     1201 gatgcgtgcg gcgatctgtt cctctcccag cctggggagg gccggtccct gcgcccggcg
     1261 cgcgaggccg ggccaggcct ggccacggga gccatcggtc cagtcgtaga aaccgccgcc
     1321 cgtcttgcgg cccaggcgcc cggcgtccac cagcatgcgc tggatggggt gcggccggta
     1381 gcgcgggtcg ccgaagaagc cctcgaacac gctctggctg acggcgaagt tgacgtccag
     1441 accgatgacg tccaccagct cgaagggccc catccggaag ccgatgccac gcattgcggc
     1501 atccacctgc tcgatgtcgg ccgtaccctc gcccaggatg cgcagcgctt ccaggtagaa
     1561 cgggcgcgcc acccggttga cgatgaagcc gggggtgtcg gcggccacca cgggggtctt
     1621 gcccagccgc cgggtgagca gggcaacctg gtcggtgacc gatgcgtccg tccgcgcccc
     1681 gggcaccacc tccaccagcg ccatcagcgg caccgggttg aagaagtgca tgccgatcac
     1741 ccgctgcgga tgctgcgcgg ccgcggcgat gcgcgcgatc gacaggctgc tggtgttgct
     1801 ggccaggatg gttgccggat cggcggcggc gtcgagcagg cggaacgcgt cgcgcttgag
     1861 ctccaggtcc tccgggatgg cctcgaccac caggtccgcg tcggcaacgt cctggatgga
     1921 gcccgctggc tggatgcagc tcaacgcctc ggagagctcc tgctcggtca gctcgtcctt
     1981 ctcgacgcgg cgcgtcaggt gcaggcgaat gcgctcgatg gctcgctcgt tggcacccgg
     2041 gatggggtcg tggacgagga cggtgaggcg cgcctcggcg gccacctgca cgatgccggc
     2101 gcccatggtg ccggcgccga gcaccccgat ccgctcgatg ccgcccagcc gcatctcgtc
     2161 agcggccaac gaaacgaggc tcccgcttct cggcgaaggc ggccacgccc tccgcgtggt
     2221 cctgggtgcg gccggcgagc tcctggaggt cagcctccag ggcaaggctc tcgtccagac
     2281 ccgcgtcctc cgccgcgttg atcagccgct tggcgtaggc cagggcgcgc gtcggcccgg
     2341 cggccagcgc tgctgcggcg tcacgcgccg tgcgcgccag ctcgctggcc ggaacgaccc
     2401 cgttcaccaa tcccacctca gccgctgccg cggcgctcaa cggctcgccg gagaagatga
     2461 gggcggcggc gcggtgccgc cccagcgcgc ggaccagcgt ccgcgtggag cccgaat
//
LOCUS       DATACA010000558         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_78156, whole genome shotgun sequence.
ACCESSION   DATACA010000558 DATACA010000000
VERSION     DATACA010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78156"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VHK28_11200"
     CDS             complement(<1..181)
                     /locus_tag="VHK28_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142818.1"
                     /translation="MSPWVLITVLLAGINLAAFLVVRGRWGRVVGLLAVASLVGAAAG
                     DAVGAAIRVEILRLGD"
     gene            complement(178..1266)
                     /gene="mnmA"
                     /locus_tag="VHK28_11205"
     CDS             complement(178..1266)
                     /gene="mnmA"
                     /locus_tag="VHK28_11205"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX2142819.1"
                     /translation="MSRVVAAMSGGVDSSVAAALLARDAAETGDEVIGVWMRLHGDSG
                     EGWEQSRSCCSPDAADDARRVAQLAGIPFFTLNLEREFGERVIDAFADGYLAGATPNP
                     CQACNQYIKFDELLRRGLAAYGADRVATGHYARLERRDGRWVLLRAADPDKDQTYFLW
                     VLDQQQLEHTLFPLGELTKPEVRRIAADLGLPTADKPESQEICFVPAGDYRQLLAERR
                     GYDAQPGPIVDADGERLGTHSGYAHYTVGQRHGLGVALGEAVYVREVRPDSNTLVVGR
                     RDEVSAGRFAVDGRRFIAGEPPAERFAASVRIRHRARDVPCEVTLVGDDRFVVETAEP
                     VWAPAPGQAAVLYDGEVCLGGGRIAVGR"
     gene            complement(1263..2435)
                     /locus_tag="VHK28_11210"
     CDS             complement(1263..2435)
                     /locus_tag="VHK28_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2142820.1"
                     /translation="MIYLDQAATTPLRPEVLDAMQPFLTEHFGNPSSIHGVGRRARQG
                     LDEARESVARTLGAKPREIVFTSGGSEADNLAIKGVAWAASGRGRHIVTSSVEHKAVL
                     HACDVLERFGFQVTRLPVDRYGRVDPADVAEAIGEHTVLVSVMYANNEVGSVQPIAEI
                     GALCRERRVLFHVDAVQAAPFLPLDVDAMQADLLSLSAHKLHGPKGVGALFVRQGTAM
                     LPQLSGGSQERQRRAGTENVPGIVGFAKALELAQAERQAEGGRLAGLRDQLIAGLSGI
                     PGVELTGHPIERLANSASFVVDGVEGGDLVAALDLEGVAASTGSACTTGAADPSHVLL
                     AMGFEPDVAHGSLRLTAGRGTTAEEVARTIEITRSVIGRLRDAMVPRPAAASVLAT"
     gene            complement(2612..>2893)
                     /locus_tag="VHK28_11215"
     CDS             complement(2612..>2893)
                     /locus_tag="VHK28_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142821.1"
                     /translation="MRIPELLDIARDSLWTNVAIGDVPNLLALGNRVSSDSIGRYQFW
                     PPEIPQDLTDGGIARIRQMVAGAFPPTAASSGASGSATAKPSPAPADGC"
BASE COUNT          406 a         1094 c         1006 g          387 t
ORIGIN      
        1 agtcgcccag ccgcaggatc tccacccgaa tcgccgctcc gaccgcgtcg ccagccgcgg
       61 cgccgaccag ggatgccacc gccaggagcc ccacgacgcg gccccaccgt ccgcgcacta
      121 cgaggaacgc cgccaggttg atgcccgcca gcaggacggt gatcaggacc cacgggctca
      181 tcggccgacg gcgatgcgcc cgccgcccag gcagacctcg ccgtcgtaca gcacggccgc
      241 ctggcctggg gccggagccc agaccggctc ggcggtctcc accacgaacc tgtcgtcacc
      301 caccagcgtc acctcacagg gaacgtcgcg agctcggtgc cgaatgcgga cgctggccgc
      361 gaaccgctca gcgggtggct ccccggcgat gaagcgccgc ccgtccaccg cgaagcgccc
      421 ggcggaaacc tcgtcgcgac ggccgacgac cagcgtgttg ctgtccgggc gcacctcgcg
      481 cacgtagacc gcctcgccca gggccacgcc caggccgtgg cgctgcccca ccgtgtagtg
      541 cgcgtagccg gagtgggtgc ccagccgctc accgtccgcg tccacgatcg gccccggctg
      601 ggcgtcgtag ccgcgccgct cggcgagcag ctgccggtag tcaccggccg ggacgaagca
      661 gatctcctgc gactccggct tgtcggcggt cggcagcccg aggtcggccg cgatgcggcg
      721 cacctccggc ttggtcagct cgcccagcgg gaagagcgtg tgctccagct gctgctggtc
      781 gaggacccac aggaagtagg tctggtcctt gtccgggtcg gcggcgcgca gcagcaccca
      841 gcggccgtcg cgtcgctcca gccgggcgta atgtccggtg gccacccggt cggcgccgta
      901 ggctgcaagc ccacgccgca ggagctcgtc gaacttgatg tactggttgc aggcctggca
      961 cgggttgggc gtggcgccgg ccaggtaacc gtcggcgaag gcgtcgatca cccgctcacc
     1021 gaactcgcgc tccaggttga gggtgaagaa cgggatgcca gccagctgcg ccacccggcg
     1081 ggcgtcgtcg gcagcgtccg gagagcagca cgaccggctc tgctcccagc cctcgcccga
     1141 gtcaccgtgc aggcgcatcc agacgccgat cacctcgtcc ccggtctcgg ccgcatcccg
     1201 cgccaaaagg gccgcggcca cgctggagtc cacgccgccg ctcatggcgg ccaccacccg
     1261 actcatgtcg ccaggaccga tgcggccgcg gggcgtggca ccatcgcgtc acgcagccgc
     1321 ccgatcactg agcgcgtgat ctcgatggtg cgggccacct cctccgcggt cgtgccgcgg
     1381 ccggcggtca gccgcaggga gccgtgagcc acatccggct cgaagcccat ggccagcagc
     1441 acgtggctcg ggtccgcggc gccggtcgtg caggcgctgc cggtggacgc ggcgacaccc
     1501 tccaggtcga gcgcggccac caggtcgccg ccctcgacgc cgtccacgac gaagctggcg
     1561 ctgttggcca gccgctcaat cgggtgcccg gtcagctcca cgccagggat gccgctcaag
     1621 cccgcgatca gctggtcgcg cagccccgcg agccggccac cctccgcctg gcgctcggcc
     1681 tgggccagct cgagcgcctt ggcgaagccc acgatgcccg gcacgttctc cgtccccgcc
     1741 cgacgctgtc gctcctggga cccgcccgac agctgcggca gcatggcggt cccctgccgc
     1801 acgaacagcg ccccgacacc cttgggccca tgcagcttgt gcgcgctcag gctgagcagg
     1861 tcggcctgca tggcgtccac gtcgagcggc aggaacgggg ccgcctgcac cgcgtcgacg
     1921 tggaacagca cgcggcgctc gcggcacagg gcaccgatct cggcgatggg ttgcacgctg
     1981 cccacctcgt tgttggcgta catgacgctg acaagcaccg tgtgctcccc gatggcctcg
     2041 gccacgtcgg ccgggtccac ccggccgtat cggtccaccg gcaggcgggt gacctggaag
     2101 ccgaaacgct ccagcacatc gcaggcgtgc agcacggcct tgtgctcgac gctgctggtc
     2161 acgatgtgcc ggccgcgccc gctggccgcc caggccacgc ccttgatggc caggttgtcc
     2221 gcctcgctgc cgccggaggt gaagacgatc tcgcgcggct tggcgcccag cgtacgcgcc
     2281 accgactcgc gcgcctcgtc gagcccctgc cgggcgcggc ggccgacgcc gtggatgctc
     2341 gacgggttgc cgaagtgctc ggtcaggaac ggctgcatgg cgtcgagcac ctctggccgc
     2401 agcggggtgg tggcggcttg gtcgaggtag atcatgggag agtcagatta tgccccactc
     2461 ggcctgtgcg gctgtgctgc acaaccgggg tctgacaggg ccgccggtgg ctcactggcc
     2521 cgccaggacc taccggcgcg cgctgtcagg gtcgattgtg cgccggtgcg gctgtgcgcc
     2581 tgtgctgcac gactcgcccc cgccactccc ctcagcagcc gtcggcaggc gccggagaag
     2641 gtttggccgt cgccgagccg gatgcccccg acgaggcggc tgtcggcggg aaagcgccgg
     2701 cgaccatctg gcggatgcgg gcgatgccac catcggtcag gtcctgggga atctcgggcg
     2761 gccagaactg gtagcgcccg atgctgtcgg acgacacccg gttgcccagc gccaggaggt
     2821 tcggcacgtc accgatcgcc acgttggtcc acagcgagtc acgcgcgatg tcgagcagct
     2881 ccgggatgcg cat
//
LOCUS       DATACA010000559         3290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1123253, whole genome shotgun sequence.
ACCESSION   DATACA010000559 DATACA010000000
VERSION     DATACA010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123253"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VHK28_11220"
     CDS             complement(<1..450)
                     /locus_tag="VHK28_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142822.1"
                     /translation="MAADARRELNATGLELLAQAAWWSGRLDVSLDARERAYSQAVKA
                     KDSTTAVVAAIGLGRDNLLRMNTTVAAAWLNRADRLLEGVPENLGHGWLAGSRAFLHA
                     LGGDNDASLAQATTAHEIAVRIGDRDLEIFALGAKASALMARGDVKEG"
     gene            complement(728..2416)
                     /locus_tag="VHK28_11225"
     CDS             complement(728..2416)
                     /locus_tag="VHK28_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2142823.1"
                     /translation="MTSTAAAHDRAPIELPHRTRIEILIAVLLGIFLSALDQTIVGTA
                     LPRIVTDLNGNDVYTWAFTSYLVTATVSGPIYGKLSDIFGRRPIFIFGVGVFLLGSIL
                     CGLSAEMWQFIAFRGLQGLGAGALFPVALAIIGDIFSPSERGKYQGFFGAVFGLSSLI
                     GPALGGLITDNVGWHWIFYVNVPIGAVVLYIIWRTLPAHHEPDADRHIDYLGASLFVA
                     ALVPILIGFTNKADAEWTDPFVGGLIGLGLALMAAFVWAESRAKEPILPLGLFRIRSF
                     TASVIAIFLAAMGFFAVIVFLPRWYQVVAGASATESGYQILPLLFGLIVGAVGSGQIV
                     ARTGRYKALIFGALLLLAAGLFLLTNLRADTPRPLIWLWMAIAGFGIGPSFAVFTLVV
                     QNAVPVRALGSATSSVTLFQQVGGTVGLAVVGSVFGSVFLDEVPRQLVGAGVPQEFAN
                     GFASGGTSTLNEIGGVGDLGAAILAQVPEAFRAQVEPMIPAIVGGIHEAFSIATAATF
                     VMGIVSALLAAFVVLVVLPRGRIGEREGVELEAGAAAAAAGEPHGEPRAATAEQ"
     gene            complement(2540..>3290)
                     /locus_tag="VHK28_11230"
     CDS             complement(2540..>3290)
                     /locus_tag="VHK28_11230"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2142824.1"
                     /translation="VARDVLMGVAPGMRTGRRFFSKVPSDPRCKLCASPFAGPVAPVL
                     GLIGKKPFPGNPKYCTFCFNRMIQKRTGAEVECSILFADVRGSTALAEKMSASEFRDA
                     MDSFFAVASRVLVDHEAFVDKFVGDEVMAFFIPAMTNEQHALRAIEAGRALLAAVAAS
                     ETPLPIGVGVNTGVAFVGTVGTEDKVEFTAVGDPVNVGARLAVAAGPGELLVSVVALQ
                     SSGLAEEGLERRALELKGKTEKTDVVVMAAP"
BASE COUNT          573 a         1162 c         1087 g          468 t
ORIGIN      
        1 tccctccttc acgtcaccac gcgccatcag agcggacgcc ttcgcgccca gggcgaagat
       61 ctccaggtcg cggtcgccga tgcggacggc gatctcgtgt gcggtggttg cctgcgccag
      121 gctcgcgtcg ttgtcgccgc ctagcgcgtg caggaaggcc cggctgccag ccagccagcc
      181 atgacccagg ttctctggca caccttccag cagacgatcc gcacgattga gccacgcagc
      241 cgccacggtg gtattcatgc gcaggaggtt gtcgcggccc agcccaatcg ctgccaccac
      301 ggccgtcgtg ctgtccttgg ccttcaccgc ctgggagtag gcgcgttcac gagcatccag
      361 cgaaacgtcg aggcgaccgc tccaccaggc ggcctgtgcc agcagttcca ggcctgttgc
      421 attgagctcc cgccgcgcgt ccgccgccat caggccatcg aaggcggcac gccactcgtg
      481 ccgttcgagc tgctcccgcg cagcgtcaag gatggggagg tccgtggtgc cgctcatcgg
      541 aagttccatt ctcccatccg ctgtcacgac ggggccgcgc gcacggggcc gccatcccga
      601 cacctgcggc tccagcgctg gccaggtgct gtcaggcccc ctttgtgcgg ctgtgcggct
      661 gtgctccttt gccgcacaaa cgacccttac agcgggtggg cgggcatgcg gccaccaccc
      721 gcaaccccta ctgctcggcg gtagccgccc tcggctctcc gtgcggctca ccagccgccg
      781 cagccgctgc cccggcctcc agctccacgc cctcgcgctc accgatccgg ccgcgcggca
      841 gcaccaccag gaccacaaac gcggcgagca gggcgctgac gatccccatc acgaaggtag
      901 ccgcggtggc gatcgagaag gcctcgtgga tgccgcccac gatggccggg atcatcggct
      961 cgacctgcgc gcggaatgcc tcgggcacct gggcaaggat ggcagcgccc aggtccccca
     1021 ccccgccgat ctcgttcagc gtggacgtgc cacccgacgc gaagccgttg gcgaactcct
     1081 gcggcacgcc ggctccgacc agctggcgcg gcacttcgtc gaggaacacg gatccgaaaa
     1141 cagagcccac gacagccagg ccgaccgtcc cgcccacctg ctggaacagc gtcacgctcg
     1201 aggtcgcgga gcccagcgcc cggaccggca cggcgttctg caccaccagg gtgaagaccg
     1261 cgaaggacgg accgatcccg aagccggcga tcgccatcca caaccagatg agcggccgcg
     1321 gggtatcggc ccgcaggttg gtcagcagga agaggccggc cgccagcagc agcagcgcgc
     1381 cgaagatcag tgccttgtag cgcccggtgc gcgccacgat ctggcctgag ccgacagcgc
     1441 ccacgatcag gccgaacagc agcggcagga tctggtagcc ggactcggtg gccgatgcgc
     1501 cggccaccac ctggtaccag cgcggcagga agacgatcac cgcgaagaag cccatggccg
     1561 ccaggaagat ggcgatcacg ctggccgtga aggaccggat gcggaacagg cccagcggca
     1621 ggatcggctc cttggcccgc gattcggccc acacgaaggc cgccatcagc gccagcccaa
     1681 ggccgatcag cccgcccacg aagggatcgg tccattccgc gtcagccttg ttggtgaagc
     1741 cgatgaggat cggcaccagg gcagcgacga acagggatgc acccaggtaa tcgatgtggc
     1801 ggtccgcgtc gggctcgtgg tgcgccggca gcgtccgcca gatgatgtac agcaccacgg
     1861 cgccgatggg cacgttcacg tagaagatcc agtgccagcc gacgttgtcg gtgatcagtc
     1921 cacccagcgc gggaccgatg aggctcgaca ggccgaacac ggctccgaag aagccctggt
     1981 acttgccacg ctcggatggc gagaagatgt cgccgatgat ggccagcgcc accgggaaga
     2041 gcgcgccggc acccaggccc tgcagccctc ggaaggcgat gaactgccac atctcggccg
     2101 aaagcccgca caggatcgag cccagcagaa acacgccgac gccgaagatg aagatgggcc
     2161 ggcgaccgaa gatgtcggac agcttgccgt agatcggccc gctgacggtg gcggtcacca
     2221 ggtacgaggt gaaggcccag gtgtagacgt cgttgccgtt gaggtcggtc acgatgcggg
     2281 gcagggcggt gcccacgatg gtctggtcca gcgcggacag gaagatgccc agcaggacgg
     2341 cgatcaggat ctcaattcgg gtacggtgcg gcagctcgat cggcgcgcgg tcgtgcgcgg
     2401 cggcggtgga cgtcatgtgg tgcggctctc cgggatgatc ggtgggggcg ggcgcgcagt
     2461 gtaccgatcc ctcggccagc cgacattggg cgcctgccgg gcagcgccta cgtcatttgc
     2521 cgcatggggt gccggttgct cagggcgccg ccatcaccac cacatcggtc ttctcggtct
     2581 ttcccttcag ctccagtgcg cgtcgctcca ggccctcctc tgcgaggccg gatgactgca
     2641 gtgccaccac gctgaccagc agttcgccgg gaccggcggc cacggccagt cgcgccccaa
     2701 cgttgaccgg gtctcccacc gcggtgaact cgaccttgtc ctcggtcccg acggtgccaa
     2761 cgaatgccac gccggtgttc actcccaccc cgatcggcag cggcgtctca gaggctgcca
     2821 ccgctgccaa gagcgcgcga cccgcctcga tcgcccgcag ggcatgctgc tcattggtca
     2881 tagccgggat gaagaaggcc atgacctcgt cgcctacgaa cttgtccacg aaggcttcgt
     2941 gatccacgag cacccgcgag gcaacggcga agaagctgtc catcgcgtcg cggaactcgg
     3001 acgcgctcat cttctcggcc agcgcggtcg agccgcgcac gtcggcgaac aggatgctgc
     3061 actccacctc cgccccggtc cgcttctgga tcatgcggtt gaagcagaac gtgcagtact
     3121 tcggattacc agggaagggt ttcttgccga tcaggcccag gaccggggcg accggtccgg
     3181 cgaacggtga tgcgcacagc ttgcaacgag ggtcggacgg caccttgctg aagaaccgac
     3241 ggccggtgcg catgccgggc gcgactccca tcagcacgtc ccgggctacg
//
LOCUS       DATACA010000560         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1284513, whole genome shotgun sequence.
ACCESSION   DATACA010000560 DATACA010000000
VERSION     DATACA010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284513"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1691)
                     /locus_tag="VHK28_11235"
     CDS             complement(111..1691)
                     /locus_tag="VHK28_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX2142825.1"
                     /translation="MSNRQILQAMSGLMAGMFVAILAGTVVSNALPRIIADLGASQSS
                     YTWVVTAELLAMTATVPLWGKLSDLYNQKLLLQLSLGLFVVGSLLAGFSHDVELLIFS
                     RVAQGIGAGGLTALAQVVMAAIIPPRRLGKYAGIFGAVFAVGTVAGPLIGGVLVDTSW
                     LGWRWCFFIGVPFALLAIALLQRTLHLPTVRREAKIDYLGAFLIVAGVCALLIWVTLA
                     GNEFDWASWQTAALTGGGVVLLVLAVLVEAKVAEPVIPLAIFRNRTVALTTVASLFVG
                     VAMFGGTVFLSQYFQVSLGRSPTIAGLMSLPMILGLLVSSTVAGQIISATGRWKVYLV
                     VGAVTMTVGLGLLSTIGTETHFGLLSVYMAVMGIGVGMLMQNLVLAAQNDVPAAELGA
                     ATSVLSFFRSMGGTIGTSVLGAILANRVTSETTKSLKDAGGAVEGGGQAGHGGVPDMT
                     ALPAPMREIVEHAYGVATAELFLVATPFAALALIAVLFIKEKPLKNTSGMERLSEESA
                     DAGRKVAGGSLPRVDTDA"
BASE COUNT          313 a          612 c          613 g          234 t
ORIGIN      
        1 tcagccacgt cctcgaaggt gacgacgcct cccactacat ccccgccgtg gcctgagcgc
       61 gacagggccg ccggaagggg acgtgcccct gccggcggcc ctggctctcg tcaggcgtcc
      121 gtgtcgacgc gcgggaggga accgcccgcg accttcctgc ccgcgtccgc cgactcctcg
      181 gagagccgct ccatgccgct ggtgttcttc agcggcttct ccttgatgaa cagcacggcg
      241 atcagcgcga gggccgcgaa gggggtggcc acgaggaaga gttcggcggt ggcgaccccg
      301 taggcgtgct ccacgatctc ccgcatcggc gccggcaggg cggtcatgtc gggcactccc
      361 ccgtgcccgg cctgccctcc gccctcgacg gcaccgcccg cgtccttcag gctcttggtc
      421 gtctcggagg tgacacggtt ggccaggatc gcgccgagca cactggtccc gatggtgccc
      481 cccatgctgc ggaagaagga caacaccgag gtcgcggcgc ccagttcggc cgccgggacg
      541 tcgttctgcg cggccagcac caggttctgc atcagcatgc cgacaccgat gcccatgacc
      601 gccatgtaga cgctgaggag accgaagtgg gtctccgtcc cgatcgtgga gagcagtccg
      661 agacccacgg tcatggtgac ggcgcccacg acgaggtaca ccttccaccg gccggtcgcg
      721 ctgatgatct gccccgcgac ggtggaggag accagcaggc ccaggatcat gggcaggctc
      781 atcagacccg cgatcgtcgg ggacctgccg agcgagacct ggaagtactg cgacaggaag
      841 accgtgccgc cgaacatggc cacaccgacg aagaggctgg cgacggtggt cagcgccacg
      901 gtgcggttgc ggaagatggc cagcggaatg accggctcgg cgaccttcgc ctcgacgagg
      961 acggcgagga ccagcagcac cactcccccg ccggtcagcg cggccgtctg ccacgacgcc
     1021 cagtcgaact cgttcccggc gagcgtgacc cagatcagca gcgcgcagac acccgcgacg
     1081 atcaggaagg cgcccaggta gtcgatcttc gcctcgcggc gaacggtggg caggtgcagc
     1141 gtgcgctgga gcagggcgat ggcgagcagc gcgaagggca cgccgatgaa gaagcaccag
     1201 cgccagccga gccacgaggt gtccaccagg acgccgccga tcagcggacc ggccacggtg
     1261 cccacggcga acacggcgcc gaagattccc gcgtacttgc ccagcctgcg cggcgggatg
     1321 atcgccgcca tcacgacctg cgccagtgcc gtgagaccac cggcaccgat gccctgggcc
     1381 acacggctga agatgagcag ctccacatcg tgggagaagc ccgccagcag cgagccgacg
     1441 acgaacaggc ccagggagag ctggagcagc agcttctggt tgtagaggtc ggacagcttg
     1501 ccccacagcg gcacggtcgc cgtcatggcc agcagttcgg cggtgacgac ccaggtgtag
     1561 gaggactggc tggccccgag gtcggcgatg atccggggca gcgcgttgga caccacggtg
     1621 ccggccagga tggcgacgaa catgcccgcc atcagccccg acatggcctg gagtatctgc
     1681 cggttggaca tggaggacgg tgacgccgct acgggcgccg ctgaaggatt cacatcatct
     1741 ctttcacggt gttgacggcg ggcgactcgc gc
//
LOCUS       DATACA010000561         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_399794, whole genome shotgun sequence.
ACCESSION   DATACA010000561 DATACA010000000
VERSION     DATACA010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399794"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VHK28_11240"
     CDS             <1..926
                     /locus_tag="VHK28_11240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2142826.1"
                     /translation="RKMVALGRSMEQNISIAIELGYLDPRDGTLVRKDALQRLRNEEL
                     VVMTTGSQGEPMSGLTRMSNRDHRNITIEPGDTVIVSASPIPGNEEAVAKTIDNLFKE
                     GAMVHYEPLVHCHVSGHGSREELKLMLGLVKPRHFIPVHGEYRMLVQHGLLAEGAGVP
                     VDGIFVLENGQVVEHQDGRARLAGNVAAGAVLVDGIAAIDGIDGVVLRDRRMLASDGV
                     VMVALTVDQATGRPVSGPDLVSRGFLHDPEDPVMIAARDHLFSELSQPPEDDHAAEPG
                     YLKTKIRDTLARFFFERTKRRPMILPIVMEV"
     gene            930..>3103
                     /locus_tag="VHK28_11245"
     CDS             930..>3103
                     /locus_tag="VHK28_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX2142827.1"
                     /translation="MAKGRATTRRRTSPRRRRANTTFSVSGRVVRELLALVLVFLAIV
                     SVIALFAPDAGFIVQPWAELLSSLLGWGIAFAGPLLAGFALMLWMKSMPSERWMAATG
                     AALVALSLLGMFHLVAGGSGELVGSGEGGGAIGFAVSALLVGAIGDAGAWIVLALLFV
                     VGLLLYFNMTIGDLVAAWLQEREERRELAEVEARRSAAAERRSRPETPAPEPEGRAGL
                     LDRVRTALGGSDEHEEPPVILRRARPEPAAAGARQAASVPREAIPVAPPEPVPTPVGG
                     TLSAEELESTEEAHLAEVGEPPPEQALLESTGRSWDLPPLNVLADAPASSAAQLDLTA
                     KGQRIRETLAHFGIGVKVSRIQEGPVVTQYALEVEPGIKLSRIEGLADNLALALSARS
                     IRIESPIPGEPFVGIEIPNSAFDLVTLKEVLSSRNFVDASERSTLAFALGQDVAGQPF
                     SADLAKMPHLLIAGATGSGKSVCVNAIICSLLMRATPAEVKLVLVDPKRVELAQYKGI
                     PHLLTEVIVDTEKAVNALKWTVGEMERRYHEFAQRGVRNIAGYNDALRPGEPRMPYIV
                     VVIDELADLMMVSAFEVEATITRIAQLARATGIHLVVATQRPSVQVITGLIKANIPSR
                     IAFAMTSGIDSRTILDTTGAEDLLGRGDMLYQPIDAPRAVRLQGVLVTDPEIEAVARH
                     WRAQGGAQYRPEITAPRRDGRAGGTPGEEEDDDADALLSQAV"
BASE COUNT          483 a         1040 c         1132 g          448 t
ORIGIN      
        1 ggcgcaagat ggtcgcgctg gggcgcagca tggagcagaa catctcgatc gccatcgagc
       61 tcgggtacct ggacccccgc gacgggactc tggtgcgcaa ggatgcgctg cagcggctcc
      121 ggaacgagga gctggtggtc atgaccacgg gctcacaggg tgagccgatg tccgggctga
      181 cccgcatgag caatcgcgac caccgcaaca tcaccatcga gcccggcgac acggtcatcg
      241 tctcggcgtc gccgatcccc ggcaacgagg aggcggtggc gaagaccatc gacaacctct
      301 tcaaggaggg cgcgatggtg cattacgagc cgctggtgca ctgccacgtc tcgggccacg
      361 gctcgcgcga ggagctgaag ctgatgctcg gcctggtgaa gccgcgccac ttcatcccgg
      421 ttcacggcga gtaccggatg ctcgtgcagc acggcctcct cgccgaggga gctggcgtgc
      481 cggtggatgg gatcttcgtc ctcgagaacg gccaggtggt cgagcaccag gacgggcgtg
      541 ccaggctcgc cggcaacgtc gcggccgggg cggtgctcgt cgacgggatc gccgccatcg
      601 acggtatcga cggcgttgtg ctccgcgatc ggcgcatgct cgcctccgat ggggtggtca
      661 tggtggcgct gaccgtcgac caggcgaccg ggcggcccgt gtcggggccc gacctggtca
      721 gtcgtggctt cctgcacgac ccggaggacc cggtcatgat cgcggcacgt gaccacctgt
      781 tctcggagct cagccagccg cccgaggacg accacgccgc cgaacccggc tacctcaaga
      841 cgaagattcg cgacacgctg gcgcgcttct tcttcgaacg aaccaagcgc cggccgatga
      901 tcctgccgat cgtgatggag gtctagagct tggccaaggg gcgagcgaca actcgacggc
      961 gcaccagccc tcgacggcgg cgcgcgaaca ccaccttcag cgtcagcggc cgcgtcgttc
     1021 gcgagctgct ggcgctggtg ctcgtgttcc tcgccatcgt cagcgtgatc gccctcttcg
     1081 ccccggacgc gggcttcatc gtgcaaccct gggcggagct gctgtcgagc ctgctgggct
     1141 gggggatcgc cttcgccggg ccgctgctgg ccggcttcgc gctcatgctg tggatgaagt
     1201 ccatgccatc cgagcgctgg atggcggcca ccggtgccgc gctggtggcg ctctcgctgc
     1261 tcggcatgtt ccacctggtt gccggcggca gcggagagct ggtgggcagc ggcgagggcg
     1321 ggggagccat cggcttcgcg gtaagcgcgc tgctggtggg cgccatcggt gatgccggcg
     1381 cctggatcgt gctggccctg ctgttcgtgg tgggcctcct gctctacttc aatatgacga
     1441 tcggggacct ggtggcggcc tggctgcagg agcgcgagga gcggcgcgag ctggcggagg
     1501 tcgaggcgag acggtccgcc gccgcggagc gcaggagccg cccggagaca ccggcgccgg
     1561 aaccggaggg acgggcgggg ttgctcgacc gcgtgcgcac ggcgctgggt ggcagcgacg
     1621 agcacgagga accgccggtc atcctgcgtc gcgcccgccc ggagcccgcc gcggccggtg
     1681 cgcgacaggc cgcatcggtt ccgcgggagg cgataccggt ggcccctccg gagccggtgc
     1741 ccacgccggt gggcgggacg ctgtcggccg aggagctgga aagcaccgag gaggcgcacc
     1801 tggccgaggt gggggagccg ccgcccgagc aggcgctgct ggaatccacc ggtcgcagct
     1861 gggacctgcc accgctgaac gtgctcgccg acgcgccggc cagcagcgcc gcgcagctgg
     1921 acttgaccgc caagggccaa cgcatccgcg agacgctggc ccacttcggg ataggcgtca
     1981 aggtgtcgcg catccaggaa gggccggtcg tcacccagta cgccctggag gtggagccgg
     2041 gcatcaagct gagccgcatc gaagggctgg cggacaacct ggccctggcc ctgtcggcac
     2101 gctccatccg catcgagtcg ccgatccccg gcgagccgtt cgtgggcatc gagatcccga
     2161 acagcgcctt cgacctggtg accctcaagg aggtgctcag cagtcgcaac ttcgtggatg
     2221 ccagtgagcg cagcacgctg gccttcgcgc tgggccagga cgtggccgga cagccattca
     2281 gcgccgacct ggccaagatg ccgcacctgc tgatcgccgg cgccaccggg tcaggcaaga
     2341 gcgtatgcgt caacgccatc atctgctcgc tgctgatgcg cgccacgccg gccgaggtga
     2401 agctggtgct ggtcgatccc aagcgggtcg agctggcgca gtacaagggc atcccgcacc
     2461 tgctgaccga ggtgatcgtc gataccgaga aggcggtcaa cgcgctgaag tggacggtcg
     2521 gcgagatgga gcggcgctac cacgagttcg cgcagcgcgg cgtgcgcaac atcgccggct
     2581 acaacgacgc cctgcgtccg ggggagccca ggatgccata catcgtggtc gtcatcgacg
     2641 agctggccga cctgatgatg gtgtccgcct tcgaggtgga ggcaaccatc acgcgcatcg
     2701 cccagctggc gcgggcgacg ggcattcacc tggtggtcgc cacccagcgg ccatcggtcc
     2761 aggtcatcac cgggctgatc aaggccaaca tcccaagccg catcgccttc gccatgacct
     2821 cggggatcga ctcgcgcacc atcctggaca cgaccggtgc cgaggatctg ctgggccgcg
     2881 gcgacatgct ctatcagccc atcgacgcgc cgcgtgccgt ccgcctgcag ggggtgctcg
     2941 tgaccgaccc cgagatcgag gccgtggcgc gtcattggcg ggcgcagggc ggtgcgcagt
     3001 accgcccgga gatcacggcg cccaggcgcg atgggcgggc gggtggcacg cccggcgagg
     3061 aggaggatga cgacgcagac gcactgctga gccaggcggt gga
//
LOCUS       DATACA010000562         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2087752, whole genome shotgun sequence.
ACCESSION   DATACA010000562 DATACA010000000
VERSION     DATACA010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087752"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1183
                     /locus_tag="VHK28_11250"
     CDS             17..1183
                     /locus_tag="VHK28_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2142828.1"
                     /translation="MAEIRLEGVTKLFKQVRAVDDVSLTIAEGEFMVLLGPSGCGKTT
                     LLRSIAGLEQIDNGRILIGDRDVTYLPPRKRNIAMVFQSYAVFPHMKVFDNIAFGLRM
                     RRTPAAEIKRQVQQAAELLHIENLLDRYPSQTSGGQRQRIAVARALAMEPQVLLMDEP
                     LSNLDALLRLEARAELKRLLSEIGSTTIYVTHDQVEAMSMGDRVAVMRTGKLVQVDDP
                     LAIYDRPADRFVGGFIGNPPMNFLPVDVQRRNGSSVAVMADAGELRLADADGLGDRTG
                     RVLMGIRAENIRIEAGAGGSGPRGRVLVVEPLGSHNLVTLQLGAEQLKAITRPDEHLK
                     MNQDVGVVIDPDRVRWLDAEREIALADDTETGPGAGMGRVSPGELDAQAPPETA"
     gene            complement(1233..2030)
                     /locus_tag="VHK28_11255"
     CDS             complement(1233..2030)
                     /locus_tag="VHK28_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2142829.1"
                     /translation="MSVRVAVAQTAPSIGEVDANVAAVERLVGSLAGSTDLLVLPELF
                     TTGYRRDGLDHAALAEPIPQGSSLQRLSRAVEAANLSLVGTVLEAADGQVYDTAVVID
                     PQGEVAATYRKTHLYPAERAHFGAGDRLVTTHVAGLHVGLAICFEHAFPEIFTELALA
                     GAELIAIPSAVPVGFEHLLDLRTRARGQDNQVFVAAANLVGFDGQTQWCGSSMIVGPR
                     GEVLARAGANSAEVIVAEIETAAISDERREEPALANRRPALYPRLHT"
     gene            complement(2027..3148)
                     /locus_tag="VHK28_11260"
     CDS             complement(2027..3148)
                     /locus_tag="VHK28_11260"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX2142830.1"
                     /translation="MPAHRRIALDLAAQIAEGRYLPGSRLPAENDLADSFSVSRGTLR
                     EALRALRADGVIEAVPGRGNFVNRGSPSRPDARRRVVGVVVPSVAQPYVSDLISVMED
                     ELHRHGYALLVGSSGSTREQQAGRVHRILEEGASGLVVYPIDYEPDLPLFDNLVSGGF
                     PLVLVDRHLVGLAVDAVLPDNAGGAYAGVAHLAQLGHRRIAFVSTDNLTTTSVAERLQ
                     GYRQALLDHGLQPDEALVLAELPLTRRWPRDLATGATGHTPLIRRFLERERPTGVFAL
                     HDYLAADVLEAAQQLGLRVPEQLAIVGFDDDPPASQLAVPLTVVRQPRERIGRLAASV
                     VVDRIQGRRTETARIVLPTELVVRASSGGPVNEATETTA"
     gene            3319..>4148
                     /locus_tag="VHK28_11265"
     CDS             3319..>4148
                     /locus_tag="VHK28_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2142831.1"
                     /translation="MIPRLHSRRTALALPIAAALVLAACTADGSPGATNGAGDGASGE
                     PSGSITVVHAWAGAEGEAFQAVVDGFNEDNPGVTVELRQVPFDDLNSQMIQQFSAGTP
                     PDVVSTLPGLIHSLAAQDLLMPLDDVWDGWVDSGEYSEAFRTIASHDGTAYGVFFKGN
                     INGLIWHRTDAMEDLGIEAPSDWDGFIDAVEQVQSGGELEPFAVGASDVWVPTQWSDA
                     FLAKVAGPDAFNGLIDGSVGWDDPAVVDAFTQFSDFVQSYWPDDALDVGFTDANCAWA
                     "
BASE COUNT          662 a         1427 c         1451 g          608 t
ORIGIN      
        1 aggaggtccg ccgcccgtgg ctgagattcg cctggagggt gtcaccaagc tgttcaagca
       61 ggtgcgcgcc gtcgacgacg tcagcctgac catcgccgag ggcgagttca tggtgctgct
      121 cggaccgtcc ggctgcggga aaaccaccct gctgcgatcg atcgcgggcc tcgagcagat
      181 cgacaacggg cgcatcctga tcggtgaccg cgacgtgacg tacctgccgc cccgcaagcg
      241 gaacatcgcc atggtctttc agagctacgc ggtcttcccg cacatgaagg tgttcgacaa
      301 catcgccttc ggtctgcgca tgcggcgcac gcccgctgcg gagatcaagc ggcaggtgca
      361 gcaggccgcc gagctgctcc acatcgagaa cctgctggac cgatacccgt cgcagacctc
      421 cggtggccag cgacaacgga tcgccgtggc tcgagcgctg gccatggagc cgcaggtgct
      481 gctcatggac gagccgctca gcaacctgga cgcgctcctt cggctcgagg cacgtgccga
      541 gctcaagcgc ctgctgagcg agatcggctc gaccacgatc tacgtgaccc acgaccaggt
      601 ggaggccatg agcatgggcg accgggtcgc tgtcatgcgc accggcaagc tggtccaggt
      661 ggacgacccg ctggccatct acgaccggcc cgccgaccga ttcgtgggcg ggttcatcgg
      721 caacccaccc atgaacttcc tgccggtcga cgtgcagcgg cggaacggaa gctcggtggc
      781 tgtcatggcc gacgccggcg agctgcggct ggccgacgcg gacgggctcg gcgatcggac
      841 cgggcgggtg ctgatgggca ttcgcgccga gaacatccgc atcgaggccg gtgccggcgg
      901 gtcggggccg cgtgggcggg tgcttgtcgt ggagccgctc ggctcgcaca acctggtcac
      961 cctccagctg ggtgccgagc agctgaaggc gatcacccgg ccggacgagc acctgaagat
     1021 gaaccaggac gtcggcgtgg tgatcgaccc ggatcgcgtc cgctggctcg acgccgagcg
     1081 cgagatcgcg ctggcggacg acaccgagac cggtcccggc gccggcatgg gccgggtctc
     1141 gcccggcgag ctcgacgccc aggctccacc ggagacggcc tgactcccct ttcgcccgcg
     1201 accgatgacg tgacccggct gcagcggccc gatcaggtgt gcagcctggg gtagagcgcg
     1261 gggcgacggt tggccagggc gggttcctct ctccgctcgt cactgatggc agcggtctcg
     1321 atctccgcca cgatgacctc cgccgaattc gcgccggcgc gagccagcac ctcgcctcgg
     1381 gggccgacga tcatgctgga tccacaccat tgggtctggc cgtcgaaccc gaccaggttc
     1441 gcggcagcca cgaagacctg gttgtcctgg ccgcgggcac gcgttcggag gtcgaggagg
     1501 tgctcgaaac cgacaggcac ggcggacggg atggcgatca gctccgctcc ggccagcgca
     1561 agctccgtga agatctcggg aaaggcatgc tcgaagcaga ttgccaggcc gacgtgcaat
     1621 ccggccacat gggtcgtgac gagacgatcg ccagcgccaa aatgggcccg ctcggccggg
     1681 tacagatgcg tcttgcgata ggtagccgca acctcgccct gcgggtcgat gacgactgcg
     1741 gtgtcgtata cctgcccgtc cgcggcctcg agaacggtgc cgaccaggga gagattcgcg
     1801 gcttcgacgg cgcggctgag ccgctgcagg gatgagccct gcgggatcgg ctcggccagc
     1861 gcggcgtgat cgagtccatc gcgccggtag cccgtggtga agagctcggg cagcaccagc
     1921 aggtcggttg agccggcgag gctgccgacc aggcgctcca ccgcggccac gttcgcatcc
     1981 acctcgccga tgctcggcgc agtctgggcg accgccacgc ggacgctcac gccgtggtct
     2041 ccgtcgcctc gttgaccggc ccaccgctcg aggcgcgaac cacgagctcg gtcggcagga
     2101 cgatgcgagc cgtctccgtg cggcgaccct gaatgcggtc caccacgaca gatgccgcca
     2161 agcgcccgat gcgctcccgc ggctggcgca ccaccgtcag cggaacggcg agctgggacg
     2221 ccggggggtc gtcgtcgaag cccacgatgg ccagctgctc cgggacgcga aggcccagct
     2281 gctgcgccgc ctccagaacg tccgcggcga ggtagtcgtg cagggcgaag acgccagtcg
     2341 gtcgctcccg ctcgaggaag cggcggatca gcggcgtgtg ccctgtggcg ccggtcgcga
     2401 ggtcccgcgg ccagcggcgg gtcagcggca gctcggcgag gaccagggcc tcgtccggct
     2461 ggaggccatg gtccagcagt gcctggcggt aaccctgcag gcgctcggca acggaggtgg
     2521 tggtcaggtt gtcagtgctg acgaaggcga tccgccgatg gccaagctgc gccaggtggg
     2581 caacgcccgc gtacgcccca cccgcattgt ccggcagcac ggcgtcgacc gccaggccga
     2641 ccaggtggcg gtcgaccagc accagcggga acccgccgga aaccaggttg tcgaagagcg
     2701 gcaggtctgg ctcgtagtcg atgggataga ccaccaggcc gctggcgccc tcctcgagga
     2761 tgcggtgcac acgcccggcc tgctgctccc gcgtggatcc gctgcttccc accagcaacg
     2821 catagccatg ccggtgcagc tcatcctcca taacgctgat gaggtccgaa acgtacggct
     2881 gcgcgacgga cgggacgacc acgcccacga cccggcgccg tgcatcgggg cggctgggcg
     2941 agccgcggtt gacgaagttc ccgcggcccg ggacggcctc gatgactccg tccgcgcgga
     3001 gcgcccgcag cgcttcgcga agcgttccgc ggctcacgct gaagctgtca gcaaggtcgt
     3061 tctcagccgg aaggcggctc cccggcagat aacggccctc cgcgatctgg gccgccaggt
     3121 cgagggcgat gcgcctatgg gccggcattc gctcgcctcg ttccgatccg ctcacgcgct
     3181 gcagtatata ccgcgtccgg caactggcaa gaggcggcgt gcacgcccac ggaagcgtgc
     3241 cagtcgacgc ggcgtaaccc tcgacaggcg gtatgagccg gtttatactg ccgcggagtg
     3301 agctaggaga cgctgcgcat gatcccccgc ttgcattcac ggcggaccgc cctcgcactt
     3361 cccatcgccg cggcactggt cctcgccgct tgtaccgcag acggctcgcc cggcgccacg
     3421 aacggcgcgg gtgacggagc cagcggcgag ccgtccggct cgatcacggt cgtccacgcc
     3481 tgggccgggg ccgagggcga ggcgttccag gcggtcgtag acgggttcaa tgaggacaac
     3541 ccgggtgtca ccgtcgagct ccggcaggtc cccttcgacg acctgaactc gcagatgatc
     3601 cagcagttct cggccggcac cccgcccgac gtggtgagca cccttcccgg gctgattcac
     3661 tccctggcgg cccaggacct gctcatgccg ctggacgacg tgtgggatgg ctgggtcgac
     3721 tccggcgagt actcagaggc gttccgaacc atcgcctccc atgacggaac cgcctacggt
     3781 gtgttcttca agggcaacat caacgggctg atctggcacc ggaccgatgc gatggaggat
     3841 ctcggcatcg aggcgccctc cgattgggac ggattcatcg acgccgtgga gcaggtgcag
     3901 tccggcggcg agctcgagcc attcgcggtg ggtgcctccg acgtgtgggt tcccacccaa
     3961 tggagcgacg cgttcctggc caaggtggcc ggaccggacg ccttcaacgg cctgatcgac
     4021 ggctcggtcg gctgggacga cccggcagtc gtggacgcct tcacccagtt cagcgacttc
     4081 gtgcagtcct actggccgga tgacgccctg gacgtcggct tcaccgatgc caactgcgcg
     4141 tgggcgag
//
LOCUS       DATACA010000563         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_160453, whole genome shotgun sequence.
ACCESSION   DATACA010000563 DATACA010000000
VERSION     DATACA010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160453"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1515)
                     /locus_tag="VHK28_11270"
     CDS             complement(250..1515)
                     /locus_tag="VHK28_11270"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2142832.1"
                     /translation="MATTQIAQATNIWAIAQSQFDHAADQLDLDPGLRRVLRVPKREL
                     TVNFPVTMDDGHVEVFTGYRVQHNLSRGPAKGGIRYHQDVTLDEVRALAMWMTWKCAV
                     VNIPYGGGKGGVIVDPKQLSQRELEGLTRRFTTELSPLIGPDRDIPAPDVNTNAQTMA
                     WIMDTYSMHHGYTISGVVTGKPIAVGGSLGRNEATARGAVFTLLRASAALNLPLTGAR
                     VAIQGYGNAGSIAAELLAAEGARIVAVSDSSGGIHNPAGLDPAKVGAWKREHGTVVGF
                     PGSDTVTNQEILELECEILVPAALENQITRANAPRIQAKVIAEAANGPTTPEADAILH
                     DRGVFMIPDILCNAGGVTVSYFEWVQDMQSFFWTEERINESLKEIMDRAFEAVHSMSQ
                     QHDVDMRTAAYMVAVARVAEATTLRGLYP"
     gene            complement(1689..>1998)
                     /locus_tag="VHK28_11275"
     CDS             complement(1689..>1998)
                     /locus_tag="VHK28_11275"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX2142833.1"
                     /translation="APIHGVRRLLDKLGIGLDEFDLIEINEAFAAQVLADGNELGFDW
                     DKVNVNGGAIALGHPIGASGARVLTTLLYELRRRGGRKGLATLCLGGGGAVALAVEMA
                     "
BASE COUNT          292 a          685 c          690 g          331 t
ORIGIN      
        1 gcaatccgcc cctggttccc tgactaaggc cgcgggttga ccgggtatgc cggttgggcc
       61 gtgctcacgg cttcgagcct gccctcagcg cacccggcta aggccgtcat gcggcgggct
      121 ctggcagggc ttcggcaaca aaattggcgg ataagctagc cgagcagccg gatctggcat
      181 gaaaccaccg cactgccgcg gcgctattcc gagggcggcg tccgcatcgg tccctctggc
      241 gccaggcctt tagggataca gccctcggag cgtcgttgcc tccgccacgc gggcgacggc
      301 caccatgtag gcggcggtcc gcatgtccac gtcgtgctgc tggctcatgg agtgcaccgc
      361 ctcgaaggcg cggtccatga tctccttgag gctctcgttg atgcgctcct cggtccagaa
      421 gaagctctgc atgtcctgga cccactcgaa gtaggagacg gtcacgccac cggcgttgca
      481 caggatgtcc gggatcatga agacgccgcg gtcgtggagg atggcatccg cctcgggagt
      541 ggtcggcccg ttggccgcct cggcgatcac cttggcctgg atccgcgggg cgttggcccg
      601 cgtgatctgg ttctcgagcg ccgccggcac caggatctcg cactccagct ccaggatctc
      661 ctgattggtc accgtgtccg agccggggaa gccgacgacc gtcccgtgct cccgcttcca
      721 ggcgcccacc ttggccgggt cgaggccggc ggggttgtgg atgccgccgc tggagtccga
      781 gacggcgacg atccgggcgc cctccgcggc cagcagctcg gcggcgatcg agccggcgtt
      841 gccgtagccc tggatggcca cccgcgcgcc tgtcagcggc aggttcaagg ccgcgctggc
      901 gcgcagcagg gtgaagaccg ccccacgcgc ggtggcctcg ttgcggccca gcgagccgcc
      961 cacggcgatc ggcttgccgg tcaccacacc gctgatggtg tagccgtggt gcatggagta
     1021 ggtgtccatg atccaggcca tggtctgcgc gttggtgttg acgtccggcg ccgggatgtc
     1081 gcggtcggga ccgatgagcg ggctcagctc ggtcgtgaac cggcgggtca ggccctccag
     1141 ctcgcgctgg ctgagctgct tggggtcgac gatcacgccg cccttgcccc cgccgtaggg
     1201 gatgttcacc acggcgcact tccaggtcat ccacatggcc agggcacgca cctcgtcgag
     1261 cgtgacgtcc tggtggtagc gaatgccgcc cttggccggg ccgcggctca ggttgtgctg
     1321 gacgcggtag ccggtaaaga cctccacgtg tccgtcgtcc atggtgaccg ggaagttgac
     1381 ggtcagctca cgcttgggaa cgcggagcac ccgtcgcagg ccgggatcga ggtcgagctg
     1441 gtccgcggcg tggtcgaact gggactgggc aatggcccag atgttcgtcg cctgggcaat
     1501 ctgtgtggtg gccatgggcg ttactcgcat ctgcctccag atctacgcgc cgcggcgcct
     1561 tcgcgaagtc gtggacgcga gagcaggccc agcgcgggct cgccgattat aggagggccg
     1621 cacgcgcggc acccccgacc aaaggtcacg caggcgtcat gccgattggc ggtgcggcga
     1681 ccgagcgtct aggccatctc caccgcgagc gcgaccgcac ccccgccgcc gaggcacagc
     1741 gtggcaagtc ccttccggcc accacgccgc cgcagctcat acaggagcgt ggtcagcacc
     1801 cgtgcgccgg atgcaccgat gggatggccg agcgcaatcg ccccaccgtt cacgttgacc
     1861 ttgtcccagt cgaagccgag ctcgttgcca tcggccagca cctgggcggc gaacgcctcg
     1921 ttgatctcga tcaggtcgaa ctcgtccagc ccgatgccca gcttgtccag caggcgccgc
     1981 accccatgga tgggagcc
//
LOCUS       DATACA010000564         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1123525, whole genome shotgun sequence.
ACCESSION   DATACA010000564 DATACA010000000
VERSION     DATACA010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123525"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..1073
                     /locus_tag="VHK28_11280"
     CDS             387..1073
                     /locus_tag="VHK28_11280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX2142834.1"
                     /translation="MGTEGGTAAELAQIASEVRACTRCPLHKGRTQAVPGEGNPLSDV
                     LLVGEGPGAREDATGRPFVGPAGQLLNELLRSVGWEREDVFIANVVKCRPPGNRDPEP
                     EEVAACGTFLHRQEQALAPAVIVTLGRHSLRRYLPGARISEAHGKLRRSGGRFVFPMY
                     HPAAALHQASLRETLFADVRGLPAALLAAREAMEAERAPSVQEEPMGTSTSEPPSSDV
                     DQLTLFEGTT"
     gene            1070..2737
                     /locus_tag="VHK28_11285"
     CDS             1070..2737
                     /locus_tag="VHK28_11285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX2142835.1"
                     /translation="MTDTLRIIPLGGVGEVGKNITVIEAAGEILVVDCGLAFPEPEML
                     GIDLVIPDATYLEQNRDRVRAIVLTHGHEDHTGALPYVLPRIPGTPIYGTKLTQGLVT
                     GKLKEHKLLSSTRLETLEPGVETRIGNMSVTPFRVNHSIPDGVGLAIRTPVGTVVHTG
                     DFKFDHTPPDGARADLGLIAEFGSRGVDFLLSDSTRAEKEGYTLSETTVGESLHQLIG
                     EASGRVIVATFASNIGRVQQVLDAAWAHGRKMVALGRSMEQNTAIAVELGYLDPRDGT
                     LARKDALQRLKPQELVIMTTGSQGEPMSGLTRMSNRDHRNITIEPGDTVIVSASPIPG
                     NEEAVAKTIDNLFKEGAMVHYEPLVHCHVSGHGSREELKLMLGLVKPRNFIPVHGEYR
                     MLVQHALLAEGAGVPVDSIFVLENGQVVEYDGTTARLAGSVPAGAVLVDGIATIDGIG
                     GVVLRDRRMLASDGVVMVALTVNSATGQAVSGPDLVGRGFLSDPEDPIMAAARDHLAN
                     ELRQGPGSDDHTAEAGYLKTKIRDTLSRFFFERTKRRPMILPIVMEV"
BASE COUNT          454 a          955 c         1005 g          383 t
ORIGIN      
        1 gtcgtcaccg agatcgaccg aatgggccgc gtcaacctgt cgcggcgcgc cgcgatggag
       61 cggcacatgg ccaagaccgg ggcgggtgcc gccagctcgt aacctgcttt cccctgcaac
      121 aggaccgatg gcgccgcctc cgagcggcgc catcggcttg tctcgaccgc gccgcactgc
      181 gggcgtgaac cgtccaaatt cacatcgagt gaccagcttg gcgttcaagc ggcctcgaag
      241 cgtccggatt cacacctcgc gtgaaattcg ggccgttcag cgccccgtag ccagtccagg
      301 tggtcacctg gcgtgaattc ggcggcttcg gagctgtgtc gatcccggcg gagcgtggca
      361 gcacgcgctt tgtacgatag ccaccgatgg gcacggaggg aggcacggcg gcagagctgg
      421 cgcagatcgc gagcgaggtg cgcgcctgca cgcgctgccc gctgcacaag gggcggacgc
      481 aggcggtgcc cggggagggc aacccgctct ccgacgtgct gctggtgggc gagggccccg
      541 gcgcgcgcga ggacgccacc gggcgtccgt tcgtggggcc ggccggacag ttgctcaacg
      601 agctgctgcg ttcagttggg tgggagcgcg aggacgtctt catcgccaac gtggtgaagt
      661 gccggccgcc gggcaaccgg gacccggagc cggaggaggt ggccgcctgc ggaaccttcc
      721 tccaccgcca ggagcaggcg ctcgccccgg ccgtgatcgt gaccctgggc cggcattcgc
      781 tgcgccgcta cctgccgggg gcgcggatca gcgaggcgca cggcaaactg cgccgatcgg
      841 gcgggcgctt cgtcttcccg atgtaccatc ctgctgcggc gctgcaccag gcgagcctgc
      901 gggagacgct gttcgccgat gtccgcggcc tgcccgcggc gctgctggcc gcccgcgagg
      961 cgatggaggc cgagcgggcg ccatcggtcc aggaggagcc gatgggaaca tcgacgtccg
     1021 agccaccatc cagcgatgtc gaccagctga ccctgtttga aggaacgaca tgacagatac
     1081 cctgcgcatc atcccgctcg gcggggtggg cgaggtcggg aagaacatca ccgtcatcga
     1141 ggcggccggc gagatcctgg tggtcgactg cggcctggcc ttcccggagc cggagatgct
     1201 cggcatcgac ctggtgatcc ccgacgcgac gtacctggag cagaaccgcg accgagtgcg
     1261 cgccatcgtg ctgacccacg gccacgagga ccacaccggc gcgctgccgt acgtgctgcc
     1321 gcgcatcccg gggacgccga tctacggaac caagctgacc caggggctgg tcaccgggaa
     1381 gctcaaggag cacaagctgc tgagctccac gcggctggag acgctcgagc cgggggtgga
     1441 gacgcgcatc ggcaacatga gcgtgacccc cttccgggtg aaccactcga tcccggatgg
     1501 ggtgggcctg gcgatcagga ccccggtggg caccgtggta cacaccggcg acttcaagtt
     1561 cgaccacacc ccgcccgatg gcgcgcgcgc cgacctgggg ctgatcgccg agttcggcag
     1621 ccgtggcgtc gacttcctgc tctccgactc gacccgcgcc gagaaggagg gctacacgct
     1681 gtcggagacg acggtggggg agtcgctgca ccagctgatc ggcgaggcgt cgggccgggt
     1741 gatcgtggcg accttcgcct cgaacatcgg tcgggtgcag caggtcctgg acgcggcctg
     1801 ggcgcacggc cggaagatgg tggcgctggg gcgcagcatg gagcagaaca cggccatcgc
     1861 cgtggagctg ggctacctgg acccgcggga cggcaccctc gcccgcaagg acgcgctgca
     1921 gcggctgaag ccgcaggagc tggtgatcat gaccaccggg tcgcagggcg aaccgatgtc
     1981 ggggctgacc cgcatgagca accgcgacca ccgcaacatc accatcgagc ccggggacac
     2041 ggtgatcgtc tccgcctcgc cgatccccgg caacgaggag gcggtggcca agaccatcga
     2101 caacctcttc aaggaggggg cgatggtgca ctacgagccg ctggtccact gccacgtctc
     2161 cggccacggc tcgcgcgagg agctcaagct gatgctgggc ctggtcaagc cgcgcaactt
     2221 catcccggtc cacggcgagt accgcatgct cgtgcagcac gccctgctgg ccgagggcgc
     2281 gggcgtcccg gtggacagca tcttcgtcct ggagaacggc caggtggtgg agtacgacgg
     2341 caccacggcg cggctggcgg gcagcgtgcc ggccggggcg gtgctggtag acggcatcgc
     2401 gaccatcgac ggcatcggcg gtgtggtgct acgtgaccga cgcatgctcg cctcggacgg
     2461 cgtggtcatg gtggccctca ccgtgaacag cgctaccggc caggctgtat ccgggcccga
     2521 cctcgtcggg cgcggcttcc tgtccgaccc ggaggacccg atcatggccg cggcccgcga
     2581 ccacctggcc aacgagctgc gccaggggcc gggttcggac gaccacaccg ccgaggccgg
     2641 ctacctcaag acgaagattc gcgacaccct gtcgcgcttc ttcttcgagc gcaccaagcg
     2701 cagaccgatg atcctgccca tcgtgatgga ggtctagggc accccggtgg cgacacgtcg
     2761 acgcacgagc acgcggcgcc gcacgacccg acgacgg
//
LOCUS       DATACA010000565         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_400171, whole genome shotgun sequence.
ACCESSION   DATACA010000565 DATACA010000000
VERSION     DATACA010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400171"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VHK28_11290"
     CDS             <1..597
                     /locus_tag="VHK28_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003881996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEX2142836.1"
                     /translation="YLPMDEAVLHCTRGIGVWDWASNDTGGEPDVVMACAGDIPTLET
                     LAAVDILRRRLPELKVRVVNVVDLMVLQDEREHPHGLPDTEFDSLFTRNKPIVFAYHG
                     YPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMVMLNDLDRFHLVIDVIDRVPDLV
                     SSAAHLRQEMIDRRLAARAYTRDHGEDDPEIRDWTWSA"
     gene            619..1712
                     /locus_tag="VHK28_11295"
                     /pseudo
     CDS             619..1712
                     /locus_tag="VHK28_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
     gene            1805..>2028
                     /locus_tag="VHK28_11300"
     CDS             1805..>2028
                     /locus_tag="VHK28_11300"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX2142837.1"
                     /translation="MDQNISLVLFSGTDDKLNAAAVMAAGAAAIGKPVHIFLQYWALD
                     AFRNDRIRRDHGLAPEAGPEGQAAVGALEKA"
BASE COUNT          293 a          713 c          719 g          303 t
ORIGIN      
        1 tacctgccca tggatgaagc ggtgctgcac tgcacgcgag gcatcggtgt ctgggactgg
       61 gcgtccaacg acaccggtgg cgagccggat gtggtcatgg cctgcgccgg cgacattccg
      121 acgctggaga cgctggccgc ggtcgacatc ctgcgccggc gcctgcccga gctgaaggtg
      181 cgggtcgtca acgtcgtcga cctcatggtg ctgcaggacg agcgtgagca tccgcacggc
      241 ctgcccgaca cggagttcga ctccctgttc acgcgcaaca agccgatcgt cttcgcctac
      301 cacggctatc cgtggctgat ccatcggctc acctaccggc gcaccaacca tgacaacctg
      361 cacgtgcgcg gttacaagga ggagggcacc accaccaccc cgttcgacat ggtgatgctc
      421 aacgacctgg accgcttcca cctggtcatc gacgtcatcg atcgcgtccc agacctggtc
      481 tcgagcgcgg cgcacctgcg ccaggagatg atcgatcgtc ggctggccgc gcgtgcctac
      541 acccgggacc acggcgagga cgacccggag atccgcgact ggacctggtc cgcctgaccg
      601 tgcacgaccg gtagaccgat gcgggtgctg gtcctcaacg ccggctccag cagcctgaag
      661 agctcgctgg tcgaggagcc gggtgaccgc accctcgtgc gtgacgagct ccagcgaccc
      721 gcaggttcgg gctggaccaa tgcgctgcgc gcggccgtgg ccgatgccgg acgggtggac
      781 gccgtcggcc accgggtggt gcacggcggt gagcgccacg tctcgccgct gctcgtctcc
      841 gacgcggtgc tggccgagct cgatgccttg aacgacctgg ctccgctgca caacggcccg
      901 gcgctggacg tgatcgcggc cgccagggaa gcactgccgc aaatcgccca cgtcgcctgc
      961 ttcgaactgc gtaccacgcc agcctgccgc tcgaggcgaa ggtctacccg ctgccctacg
     1021 cgtggtacga gcagtggggc gtgcggcgct acggcttcca cggcctgtcg gtggcgtggt
     1081 cggtggaacg ggccgcggcg ctgcttggcc ggccgcccgg ggagctgcgc ctcgtggtgg
     1141 cgcacctggg gagtggctgc tcggtcaccg ccgtggacgg cggacgctcg gtggacacct
     1201 cgatgggatt cacgccgctc gagggcctga tgatgggcac ccgcgcgggc agcatcgatc
     1261 cgggcatcat cctggagctg ctcgactccg ggcggctgga cgtccccggc ctgcgatcgg
     1321 ccctgcacca cgaatccggc ctgtggggcg tctccggcgt gagcgccgac ctccggaggg
     1381 tgcaggccgc cgcggccaac ggtgatccgc gcgccgcgct ggccctggag atgttcgtgc
     1441 gccgcgcagc tgcggggatc gcggcggcgt ccaccgcgct gccgaccctg gacgcgctgg
     1501 tcttcaccgg cggcatcggc gagcacgccg ccgagatgcg cgcggacatc tgcgggcgcc
     1561 tgggcgggct tggcgtgcca tcggtcctga atccaccggg ggaggaggac gacgtggacc
     1621 tcagcctgcc cggctcgcgc gtccgcgtgc tgcgggtggt ggcacgcgag gacctggtca
     1681 tcggccgggc agtggcccac ctgctcgact gacgcgcggc ggttgggccg atcggctcac
     1741 ccggacgcga cctccggcac tgatgcgcag atgggcatgg tccgacacta gggtcgtgag
     1801 tgacatggac cagaacatct cgctagtcct cttctcgggc accgacgaca agctcaacgc
     1861 cgcggccgtc atggctgccg gcgcggccgc catcggaaag cccgtccaca tcttcctgca
     1921 gtactgggcc ctcgacgcct tccggaacga ccgaatcagg cgcgaccacg gcctggctcc
     1981 cgaggcaggc cccgaggggc aggcagcggt tggggccctg gagaaggc
//
LOCUS       DATACA010000566         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_78765, whole genome shotgun sequence.
ACCESSION   DATACA010000566 DATACA010000000
VERSION     DATACA010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78765"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1194
                     /locus_tag="VHK28_11305"
     CDS             166..1194
                     /locus_tag="VHK28_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142838.1"
                     /translation="MGRVLGLLAVIGLLTTILPATVVAAPPTRASGWQSGILCELPSD
                     IGFVSVYVEVTEAGSFASMAIWTPEQDPFEDLPRIMSEQGSADFNGSILTAGFDLVFV
                     EEPTDPEQPPLTEPAGTARVLAALTPSGPEQDFGSRDIRDGNRWLRIDETLQLYSVDG
                     DLTIELLDGSGAVLGLESCGSSLYTVDVFGTDPNAWTYGGEQVFISCEWVTDLGTIQL
                     LAVSEEGNQQGEVIVVLKDTVLVGLTSPELSETSFTAYGELVDVSQGMPVGTVTAAAR
                     LTPTLDRISDHTWSDPHRFTVVGSQLTVNGSLTLDLFGATTVLDINDPSCDAGSVRYQ
                     VLEKMPHR"
     gene            1291..2388
                     /locus_tag="VHK28_11310"
     CDS             1291..2388
                     /locus_tag="VHK28_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX2142839.1"
                     /translation="MTDDSRAYSPGLEGVLAGETALSLVDGANGRRLYRGYPIAELVE
                     RGTFVQVAELLWTGEWPSVAHLACAPLPPQVEATIRALPRETPPMDALRTAVSAWGAV
                     SRPGWPPTVEEARAVTALSPTALAAFARLRDGEEPIPPDPELQLAAGFLYQLTGERPD
                     PAAARALDAYFVVGAEHGFNASTFVARVVTSTRSDLASAVAAAIGALKGPLHGGAPSE
                     VVNQLHDMGSVEQAEAWMRAALERGERLMGFGHRVYRAYDPRAAALRSVSEGLAGSAD
                     WLRMAVRVEEIALRLLAEHKPHRPLKTNVEFYAAAVLQGIGLTPSLFPATFALARHVG
                     WTAHALEQGGANRLIRPDVRYIGPGERALPA"
     gene            complement(2405..2881)
                     /locus_tag="VHK28_11315"
     CDS             complement(2405..2881)
                     /locus_tag="VHK28_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142840.1"
                     /translation="MTDREPSESRPRRRRARGLLPLPAALGLLAAVFVGALLAPIPTM
                     PAASDARQVAERVGQRLPGWQVDRMLSSWEGAWTVVASCGNRQVGFQFVPGHGLRPGD
                     AWLHPQDEFTRTRLRFVSDDDNYLIWYGDEQRPRSLACNSELARRADEDRRSGRLD"
BASE COUNT          437 a         1050 c         1021 g          437 t
ORIGIN      
        1 gtctagccgc ccgccaggca agcccctacg gcgtcacgcc ggtcgcccta cgtccaacgt
       61 cgcggccgcg gctgcgtcgt tcgcggcgcg ccgcggaaac gtccctggtt gtctcatgcg
      121 aggcagggag cgacgacgct ccacagcacc aacttgaggt gaatcatggg gcgcgtactc
      181 gggctgctgg ccgtcatcgg cctgctcacc accatccttc cggccaccgt ggtggccgct
      241 cctccaacca gggcctcggg ctggcagtcc gggatcctct gcgagctccc gtctgacatc
      301 ggcttcgtga gcgtctacgt cgaggtcacc gaggcgggca gcttcgcctc gatggcgatc
      361 tggacgcccg agcaggatcc cttcgaggac cttccccgca tcatgagcga gcagggcagt
      421 gcggacttca acggcagcat cctgaccgcc ggcttcgacc tggtcttcgt ggaggagccc
      481 accgacccgg agcagccgcc gctgaccgag cccgccggca cggctcgcgt gctggccgcg
      541 ctgactccct ccggcccgga gcaggacttc ggctcacgcg acatccgtga cggaaaccgc
      601 tggctgcgca tcgacgagac gctccagctg tactccgtgg acggggacct gaccatcgag
      661 ctgctggacg gcagcggcgc cgtcctgggc ctggaaagct gcggctcgag cctgtacacg
      721 gtggacgtct tcggcaccga cccgaacgcc tggacctacg gcggcgagca ggtcttcatc
      781 tcctgcgagt gggtgaccga cctgggcacc atccagctgc tggcagtcag cgaggaaggc
      841 aaccagcagg gcgaggtgat cgtggtcctc aaggacaccg tcctggtggg gctcacaagc
      901 ccggaactga gcgaaacctc gttcacggcc tacggcgaac tggtggacgt tagccagggc
      961 atgccggtgg gcaccgtaac ggccgctgcc cggctgacgc ccacactgga tcggatcagc
     1021 gaccacacct ggtcggaccc gcaccggttc acggtggtcg gcagtcagct gactgtgaac
     1081 ggcagcctga cgctggacct ctttggggcg accacggtcc tggacatcaa cgatcccagc
     1141 tgcgatgccg gttcggtccg ctaccaggtg ctggagaaga tgccgcaccg ctgaggaagc
     1201 ttccgtcgag cagcaggggc ccggtcagca cgaccgggcc cttcggcctg cccgcagaac
     1261 cgatgcatcg gtctagactc agctctatcc atgaccgatg actcccgggc gtactcgccc
     1321 ggcctcgagg gcgtgctggc cggcgagacc gccctgtcgc tcgtcgacgg cgccaacggc
     1381 cgccggctgt accgcggcta cccgatcgcc gagctggtgg agcgcggcac cttcgtgcag
     1441 gttgccgagc tgctgtggac gggggagtgg ccgtccgtcg cgcacctcgc ctgcgcgccg
     1501 ctcccgccgc aggtcgaggc gaccattcgc gcgctgccgc gcgagacgcc tcccatggat
     1561 gcgttgcgca cagccgtatc ggcttgggga gcggtcagcc ggccgggctg gccgccgacg
     1621 gtggaggagg cacgcgccgt aaccgccctc tcgcccaccg cactggccgc attcgcacgc
     1681 ctgcgagacg gcgaggaacc catcccaccc gaccccgagc ttcagctggc cgccggcttc
     1741 ctctaccagc tcaccggcga gcgacccgac ccggcggcgg ctcgggcact ggacgcgtac
     1801 ttcgtggtcg gcgcggagca cggcttcaac gcctccacct tcgtggcccg ggtcgtgacc
     1861 tcgacccgct cggacctcgc ctcggcggtg gccgctgcga tcggggcgct caaggggccg
     1921 ctgcacggcg gggcaccgtc ggaggtcgtg aaccagcttc acgacatggg atcggtcgag
     1981 caggcggagg cgtggatgcg cgctgcgctg gagcgtgggg agcggctgat gggcttcggc
     2041 caccgtgtct accgcgccta cgatccacgt gccgccgcgc tccgctcggt gtcggaaggc
     2101 ctggccgggt ccgcggactg gctgcgcatg gcggttcggg tcgaggagat cgcgctgcga
     2161 ctcctggccg agcacaagcc gcaccggccg ctcaagacga atgtggagtt ctacgcggcc
     2221 gcagtgctgc agggcatcgg cctgacgcca agcctgttcc cggccacctt cgccctggcc
     2281 cgccatgtcg ggtggacggc gcacgcgctg gagcagggcg gcgccaaccg gttgatccgg
     2341 ccggacgtgc ggtacatcgg tcccggggag cgagccctac cggcctagct ttaggggccg
     2401 gtcctcagtc gagccggccg ctgcgccgat cttcgtcggc ccgccgggca agctcgctgt
     2461 tgcaggccag cgatcgcggc cgctgctcgt cgccgtacca gatcaggtag ttgtcatcgt
     2521 ccgagacgaa ccgcagccgc gtccgcgtga actcgtcctg cggatgcagc caggcgtcgc
     2581 ccgggcgcaa tccgtggccc ggaacgaact ggaagcccac ctgccggttc ccgcacgagg
     2641 cgacgacggt ccaggcaccc tcccacgagc tgagcatccg gtcgacctgc cagcccggca
     2701 gccgctgtcc gacacgctcc gccacctggc gcgcgtccga cgccgctggc atggtgggga
     2761 tgggagccag cagcgcgccg acgaatacgg cggccagcag gcccaaggca gcgggcagcg
     2821 gcaggaggcc gcgtgcgcgt cggcggcgcg gccgagactc ggatggctcg cggtcggtca
     2881 tcgttggctc aaccatctgg acgcggtgga tctaccgatt tcggtcaggc agcatagagc
     2941 aggat
//
LOCUS       DATACA010000567         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1203792, whole genome shotgun sequence.
ACCESSION   DATACA010000567 DATACA010000000
VERSION     DATACA010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203792"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VHK28_11320"
     CDS             complement(<1..192)
                     /locus_tag="VHK28_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase"
                     /protein_id="HEX2142841.1"
                     /translation="MGPERFVHAWLLKLDPAWRRLPSTERRDDLDAFCAAMDRVRDRV
                     VQHEYSTLGLRAEGDLLLWR"
     gene            complement(192..1223)
                     /gene="hemB"
                     /locus_tag="VHK28_11325"
     CDS             complement(192..1223)
                     /gene="hemB"
                     /locus_tag="VHK28_11325"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX2142842.1"
                     /translation="MTGTIQRAAPAAERGVALRGRRLRATGPMRALVRETRLHPDMLV
                     QPLFVHAGSGSQPVGAMPGQSRIGLADGLPEVAYQLAASGVRSALLFGLPNEKDPIGS
                     GAWAEDGVMQTSIRLLRHASPDLLVIADVCLCEYTSHGHCGVLVGEGEASIVDNEATV
                     QLLARTAVSLADAGANVVAPSAMMDGQVAAIRQALDAAGHEDTAILAYAAKHASAFYG
                     PFREAAASAPAFGDRRAYQMDPANAREALREVEADVAEGADMVMIKPALPALDLVARA
                     RERVTVPLAAYQVSGEYSAICAAAQNGWLDRRASALETLTAIARAGADFIVTYFAAEA
                     ARWLREDAG"
     gene            complement(1220..1969)
                     /locus_tag="VHK28_11330"
     CDS             complement(1220..1969)
                     /locus_tag="VHK28_11330"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX2142843.1"
                     /translation="MKTLLITRPAAQAHELAAHLRERGIGSVCVPTVEIQPDVESLRT
                     SLTMLDQAAWLVITSTNGAEALARATSGSNVRVPSHLRLAAVGPATAGALRRLGFTVD
                     YVPHRYLTSAIADGLGDVRGRLVVLARADAASPALSRELGQRGAIVHDVVAYRTVEAP
                     AGSRDMAADALEQPLDGITFTSGSTARGLVSLLPPEQRTRLTALPAYCIGPVTSAVAR
                     SLGFTTPVVASTHTAAGLAEAIYTHLKEESR"
     gene            complement(1966..>2691)
                     /gene="hemC"
                     /locus_tag="VHK28_11335"
     CDS             complement(1966..>2691)
                     /gene="hemC"
                     /locus_tag="VHK28_11335"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2142844.1"
                     /translation="VGDGQFTAELDQALVNGTIDIAVHSLKDLPTLVAPGLCTAAILP
                     RGDARDALLSRHPGGLHELPFGARVGTGSPRRGAQLAAVRSDLVARAIRGNVDTRMRR
                     LEAGEYDAIVLAVAGLERLGIDVSPEQVLPPEVMLPAPGQGAIALQVRAQDGSLIRRL
                     ARVDHEPTRVAVEAERNLLRAVGGGCLAPLGALGEVQDGRLRLRAVYETKDGMVTADV
                     AGPVDRSAALVAEAAVCLLEATQ"
BASE COUNT          411 a          966 c          946 g          368 t
ORIGIN      
        1 gcgccacagg aggaggtcgc cctcggcccg cagcccgaga gtcgagtact cgtgctgcac
       61 cacgcggtct cgtactcggt ccatcgcggc gcaaaaggca tccaggtcgt cgcggcgctc
      121 ggtcgacggg agccgcctcc aggcgggatc gagcttgagc agccaggcgt ggacgaagcg
      181 ctccggaccc atcagccggc atcctcgcgc agccagcgtg cggcctcggc ggcgaagtag
      241 gtgacgatga agtcggcgcc ggcgcgtgcg atggccgtca gcgtctcaag tgccgatgca
      301 cgcctgtcca gccagccatt ctgggcagcg gcacagatcg ccgagtactc accgctgacc
      361 tggtaggcgg ccagcgggac ggtgacgcgt tctcgtgcgc gggcgaccag gtccagggcg
      421 ggaagggcgg gcttgatcat gaccatgtcg gcaccttcgg cgacgtccgc ctccacctcg
      481 cggagcgctt cgcgcgcgtt ggccggatcc atttggtacg cgcgacgatc cccgaacgcc
      541 ggagcggacg cggccgcctc gcggaagggg ccgtagaagg cggacgcatg cttggccgcg
      601 tacgccagga tggccgtgtc ctcatggccg gcggcatcca gcgcctggcg gatcgcggcg
      661 acctggccgt ccatcatcgc gctgggcgcc acgacgtttg ccccggcgtc cgccagggac
      721 acggcggtcc gggcgagcag ctggacggtg gcctcgttgt cgacgatcga tgcctcacct
      781 tcgccgacga ggacgccgca gtggccgtgg ctcgtgtatt cgcacaggca gacgtcagcg
      841 atcaccagga ggtccgggct ggcgtggcgc agcaaccgga tggacgtttg catgacgccg
      901 tcctcggccc acgcgccgga gccgatcgga tccttttcat tcggaaggcc gaagagcagc
      961 gcgctgcgca cgccagatgc cgcaagctgg taggcgacct ccggaaggcc atcggccagc
     1021 ccgatgcggg actgaccggg catcgccccg accggctggc tgccgctgcc cgcgtggacg
     1081 aacagcggct gtaccagcat gtcgggatgg agccgcgtct cacgcacgag ggctcgcatc
     1141 gggcctgtcg cgcgcagccg gcgtcctcga agcgcgacgc cgcgctcggc ggcaggcgcg
     1201 gcccgctgaa tggtgccagt catctcgact cctctttcag gtgggtatag atcgcctcag
     1261 ccaggccggc ggccgtgtgc gtactggcca ccaccggcgt ggtgaagccg aggctccgcg
     1321 cgacagccga cgtgacgggc ccgatgcaat aggcaggcag ggcggtcagg cgcgtccgct
     1381 gctccggagg cagcagcgac accaggccgc gcgcagtcga gccgcttgtg aaggtgatgc
     1441 cgtccaacgg ctgctccagc gcgtcggccg ccatgtcacg cgagccggcg ggcgcctcaa
     1501 cggtgcggta ggcgacgacg tcgtggacga tggctccgcg ctgtcccagt tcccgactga
     1561 gggcgggtga ggcagcatcg gcacgggcca ggaccaccaa ccggccgcgc acgtccccca
     1621 ggccgtcagc gatcgccgag gtcaggtagc gatggggcac gtagtcgacg gtgaagccca
     1681 ggcggcggag cgccccggcg gtggccgggc caacggccgc gagccgaagg tgcgacggaa
     1741 cccgcacgtt cgaaccgctc gtcgctcggg cgagagcctc cgcaccattt gtgctggtga
     1801 tcaccagcca cgccgcctga tccagcatgg tgagcgatgt ccgcaggctc tccacgtcgg
     1861 gctggatctc gacggtggga acgcacacgg agccgatgcc ccgctcgcgc agatgcgcgg
     1921 ccagctcgtg ggcctgcgcg gccggccgcg tgatcaggag cgtcttcact gcgtcgcctc
     1981 gaggagacac accgcagcct cggccaccag cgccgccgac cgatccacgg gccctgccac
     2041 gtccgcagtg accatcccat ccttcgtctc gtacacggct cgcagtcgca gccggccgtc
     2101 ctgcacctcc cccagggcgc ccagcggagc aagacagcct ccgccgaccg cgcgcagcag
     2161 gttgcgctcg gcttccacag ccacgcgggt cggctcgtga tcaaccctgg cgaggcgcct
     2221 gatcagcgac ccgtcctggg cgcgaacctg aagggcgatt gctccctgcc ccggcgccgg
     2281 cagcatgacc tccgggggaa gcacctgctc cggggacacg tcgatcccga gccgttccag
     2341 cccggccacc gccagcacga tggcgtcgta ctccccagcc tccaggcgcc gcatcctcgt
     2401 gtcgacgttg ccgcggatgg ccctggccac caggtcggac cgcacggccg ccagctgtgc
     2461 gccgcgcctg gggctgcccg tgcccacccg agccccgaag ggaagttcat gcagaccgcc
     2521 gggatgtcgg ctcagcaggg catcccgggc atcgcctcgc ggcaggatgg cggcagtaca
     2581 caggcccggg gccacgagag tcggcaggtc cttcagtgag tggaccgcga tatcgatcgt
     2641 gccgttcacg agcgcctggt ccagctcagc ggtgaactgt ccatccccca c
//
LOCUS       DATACA010000568         4505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_78797, whole genome shotgun sequence.
ACCESSION   DATACA010000568 DATACA010000000
VERSION     DATACA010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78797"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /locus_tag="VHK28_11340"
     CDS             <1..1958
                     /locus_tag="VHK28_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX2142845.1"
                     /translation="VALTTSSVEVLFSEEVERESAEAAFQIAPPVEGAYSWSGSTLVF
                     TPTQRLPLETEFSVSVGPGVRDLAGNEMPEASVPFEFATVGNPTVVAATPEDGESDVA
                     LDTQVVLEFSTLMDTASVESALTTVPIFEAEASWSGETLTLTPVEPLEENTRYTITIG
                     TAASDGAGNSLEEPYRLNFRTAVSALEPLVLVPADGVEGAAVTSSIAVILDTEVDADT
                     LTDDALTLEPSVAGSLSVTSQPGAAGLRQPADRMLIFNPSAPLAPNTTYEVTLAGDLA
                     GTDGSRLAGPVSWRFTTGSPLTTLSNQIVFLSERGGISNLWAMNPDGSAARQVSAELS
                     PVSSYAVAPDGRTVILGDGAILVRQRADGSGREVLTPDGVLEVDPAWAPNGQRFAFGR
                     IDAPTGTGLGLWTRDADGGDASELELPAELRATPQPTGEDDEREPVLRAPRYSPDGSA
                     LAFVTDSGRVGVLELPGGRVTTAGFTATSPPAWLTDSSAVLVAGLEGELAMGVEAGEP
                     LPELDPAALELSGVQLGSQVLVQLDRGDSSVRDLQRDGSPARPVAGVDGGYLYIALQP
                     GAPLAGGELRLVDADSDRERAPLDDSGPAATSATPGLEPDSLVVARAARTNEPGGASR
                     AGIWLVDARDGRSEQLSSDGWSPRWLP"
     gene            2140..4374
                     /locus_tag="VHK28_11345"
     CDS             2140..4374
                     /locus_tag="VHK28_11345"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019176822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX2142846.1"
                     /translation="MQLQQLTWLVPVAGLAAVAFAMYLAWDVMRRDKGPQAMQDVGDT
                     IREGADAFVRRQYTTIGVLALVGAVVIGVVIALVETREVADVPDLAGGPIGIMTAVAF
                     LVGAACSMASGIIGMFVSVRSNVRTAAAARRSLVEAVQVAMRGGAVSGFLVVALSLLG
                     VWAIFTVYETLIGDVTIAEAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVGK
                     VEAGIPEDDPRNAGVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVAAFTIAEA
                     AGWPNPEGWIFFPLVVRAFGLLATIVAMFFIRGSETEDPMNILNRGYWVTTLLSVVGL
                     FITTNLMMNTGDAVGALGWPAWVWFFGAGLVGLATSVVFVYITQYYTAGTFRPVREIA
                     EASKTGPATNIISGTAVGFETTFVTAVSIGIALIASHWLGSQAGLTNAEGNDVGGIFG
                     TAVATMGMLMTTAYILAMDTFGPITDNAGGVAEFSRSEASAREITDRLDAVGNTTKAL
                     TKGYAIASASLAAFLLFSAYIDKVNLILGNQIDAGTRAAGTLLTSVNLADVGVFVAAL
                     IGAMLVYFFSSLAIRAVGVTAQSIIIEVRRQFREMPGIMDYSQRPDYARVVDITTRAA
                     LRQMVAPGLVAVATPIIVGLLLGHEAVAGMLMVGTIAGVLLATVLNNGGGAWDNAKKF
                     IEAGHLRGEDGSVLGKGTPAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAP
                     LFIR"
     gene            4387..>4505
                     /locus_tag="VHK28_11350"
     CDS             4387..>4505
                     /locus_tag="VHK28_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX2142847.1"
                     /translation="MDVVIQAAIVGLLQGLTEFIPISSSAHLELLPWMAGWESG"
BASE COUNT          616 a         1579 c         1609 g          701 t
ORIGIN      
        1 atgtcgcgct gaccaccagc agcgtggagg tcctcttcag cgaggaggtg gaacgggagt
       61 ccgcggaggc ggccttccag atcgcgccgc cggtggaggg cgcttactcg tggagcggct
      121 cgaccctggt cttcacgccc acccaacggc tgccgcttga gaccgagttc agcgtatcgg
      181 tggggcccgg ggtccgcgac ctggctggca acgagatgcc ggaggcatcg gtcccgttcg
      241 agttcgccac cgtcggcaac ccgaccgtgg tggccgccac gcccgaggac ggggagagcg
      301 acgtggcgct ggatacccag gtcgtgctcg agttctcgac actgatggac accgcctcgg
      361 tggagtcagc gctgaccacg gtgcccatct tcgaggcgga ggcgtcgtgg agcggcgaga
      421 cgctgaccct cacgccggtc gagccgctgg aggagaacac ccgctacacg atcaccatcg
      481 gcacggcggc cagcgacggc gccggcaatt cgctggagga gccctaccgc ctcaacttcc
      541 gcaccgccgt ctcggccctg gagccgctgg tcctggttcc ggccgacggc gtcgagggcg
      601 ccgcggtgac ctcgtccatc gccgtcatcc tggacacgga ggtggatgcg gacacgctga
      661 cggacgacgc cctgaccctg gagccatccg tggccggatc gctgtccgtg acgagtcagc
      721 cgggggccgc cggcctgcgc cagcccgctg accgcatgct gattttcaac ccgtccgccc
      781 cgctggcacc caacacgacc tacgaggtga ccctggccgg tgacctggca gggaccgatg
      841 gttcccggtt ggcgggcccc gtctcctggc gcttcacgac cggatcgccg ctcaccacgc
      901 tgagcaacca aatcgtcttc ctctcggagc gcggcgggat atcgaacctg tgggccatga
      961 acccggacgg cagcgccgca cgccaggtct cggccgaact gtcgccggtg tcatcgtacg
     1021 cggttgcgcc ggacgggagg acggtcatcc tgggcgacgg tgccatcctg gtcaggcagc
     1081 gggcggatgg cagcggccgg gaggtgctca cgcccgatgg cgtcctggag gtcgatcccg
     1141 cctgggcacc caacgggcag cgcttcgcct tcggccggat tgacgcgccg accggcacgg
     1201 ggctcggcct gtggacgcgt gacgccgacg gcggcgacgc cagcgagctg gagctcccgg
     1261 ccgagctccg cgccacaccg caacccaccg gcgaggacga tgagcgtgag ccggtgctgc
     1321 gcgcgccgcg ctactcgccg gacggcagcg ccctggcctt cgtgaccgat tccggccgcg
     1381 tcggcgtgct ggagctgcct ggcggccggg tcaccacggc tggtttcacc gccacctcgc
     1441 caccggcctg gctgaccgat tcgtccgccg tcctggtcgc cgggctggag ggcgagctgg
     1501 ccatgggcgt ggaggccggc gagccactcc ccgagctgga tcccgcggcg ctggagctga
     1561 gcggcgttca gctcggctcg caggtgctcg tccagctcga ccgcggcgac tcgtcggtca
     1621 gggacctgca gcgcgacgga agcccggcgc gcccggtggc cggcgtggac ggtggatacc
     1681 tgtacatcgc gctgcagcct ggcgcgccgc tggccggcgg cgagctgcgc ctggtggacg
     1741 cggacagcga ccgcgagcgg gcgccgctcg acgattcggg accggcagcg acgtcggcga
     1801 ctcccggact cgagccggac tcgctggtgg tcgcgcgcgc ggctcgcacc aacgagccgg
     1861 gcggagcgtc acgggccggc atctggctcg tggacgccag ggacggccgc agcgagcagc
     1921 tgagctccga cggctggtcg ccccgctggc tcccgtagcg cgcgtgcgct gaaagttgac
     1981 ccgccgcacg gggccgggct acgataccgc ggcctcgctg gcgggtgctc ggcgcatctc
     2041 cgccggcgtt ttccgtgtgt tcagaccggt tcgggttgag ccgggtgcgc tctggagctt
     2101 ccggaagctg caccggaccc gaccacaatc ggaggtttca tgcagcttca gcaactcacc
     2161 tggctggtcc cggttgccgg cctggcagcc gtcgcgttcg ccatgtacct ggcgtgggac
     2221 gtcatgcggc gcgacaaggg cccgcaggcc atgcaggacg tgggcgacac gatccgcgaa
     2281 ggtgcggacg ccttcgtgcg gcggcagtac acgaccatcg gcgtcctggc cctggtcggg
     2341 gcggtggtga tcggcgtggt gatcgccctg gtcgagacgc gcgaggtggc cgacgtgccg
     2401 gacctggcgg gtggacccat cgggatcatg actgcggtcg ccttcctggt gggcgccgcg
     2461 tgctccatgg catcgggcat catcggcatg ttcgtgagcg tccgctccaa cgtgcggacc
     2521 gccgccgccg cgcgccgcag cctggtggag gcggtgcagg tcgccatgcg cggcggcgct
     2581 gtctcgggct tcctggtggt cgcgctcagc ctgctcggcg tgtgggccat cttcaccgtc
     2641 tacgagaccc tgatcggtga cgtgaccatc gccgaggcgc cgttcctgat cgtcggcttc
     2701 ggcttcggcg cctcgttcgt ggcgctcttc gcgcagctcg gcggcgggat ctacaccaag
     2761 gccgcggacg tgggctccga cctggtcggc aaggtggagg ccggcatccc ggaggacgac
     2821 ccgcgtaacg ccggcgtcat cgccgacctg gtgggcgaca acgtgggtga ctgcgccggt
     2881 cgtggcgccg acctgttcga gtcgaccgcc gccgagaaca tcggggccat gatcctgggc
     2941 gtcgccgcct tcaccattgc cgaggccgcc ggctggccca accccgaggg ctggatcttc
     3001 ttcccgctgg tggtgcgcgc cttcggcctg ctggccacca tcgtggccat gttcttcatc
     3061 cgcggcagcg agaccgagga tccaatgaac atcctcaacc gcggctactg ggtcaccacc
     3121 ctgctgtcgg tggtggggct gttcatcacc accaacctga tgatgaacac cggcgacgcg
     3181 gtcggtgccc tcggctggcc ggcctgggtc tggttcttcg gcgccggcct cgttggtttg
     3241 gccaccagcg tggtcttcgt ctacatcacc cagtactaca cggcgggcac cttccggccc
     3301 gtgcgggaga tcgccgaggc ctcgaagacc ggcccggcca ccaacatcat cagcggcacg
     3361 gcggtcggct tcgagacgac cttcgtgacc gccgtgtcca tcggcatcgc gctgatcgcg
     3421 tcgcactggc tgggcagcca ggcgggcctg accaacgccg agggcaacga cgtcggcggc
     3481 atcttcggca ccgcggtggc caccatgggc atgctgatga ccacggccta catcctggcc
     3541 atggacacct tcgggcccat caccgacaac gctggtggcg tggccgagtt cagccgctcc
     3601 gaggccagcg cccgcgagat caccgaccgc ctggacgcgg ttggcaacac gaccaaggcg
     3661 ctgaccaagg gctacgccat cgccagcgcc agcctcgcgg ccttcctgct gttcagcgcc
     3721 tacatcgaca aggtcaacct gatccttgga aaccagatcg acgccgggac gcgcgcggcg
     3781 ggcaccctgc tgacctcggt caacctggcg gacgtggggg tcttcgtggc ggccctgatc
     3841 ggcgccatgc tggtctactt cttcagctcg ctggccatcc gggcggtggg cgtcaccgcc
     3901 cagtcgatca tcatcgaggt gcgccgccag ttccgcgaga tgcccggcat catggattac
     3961 agccagcggc cggactatgc gcgggtggtg gacatcacga cccgggcggc gctgcggcag
     4021 atggtcgccc ccgggctggt ggcggtggcc acgcccatca tcgtcggcct gctgctgggc
     4081 cacgaggcgg tggccggcat gctgatggtg ggcaccatcg ccggcgtcct gctggccacg
     4141 gtgctgaaca acggtggtgg cgcctgggac aacgccaaga agttcatcga ggccgggcac
     4201 ctgcgcggcg aggacggcag cgtcctgggc aaggggacgc cggcccacgc ggccgccgtg
     4261 gtgggcgaca ccgtcggcga tcccttcaag gacaccgcgg gcccgtcgct gcacgtcctg
     4321 gtgaagctgc tcgccacgat caccctggtg ctcgccccgc tgttcatccg gtaaggcggc
     4381 gccgcgctgg acgtcgtcat ccaggccgcg atcgtcgggc tgctgcaggg gctgaccgaa
     4441 ttcatcccga tcagctcctc ggcgcacctc gagctgctgc cgtggatggc ggggtgggag
     4501 tccgg
//
LOCUS       DATACA010000569         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_642044, whole genome shotgun sequence.
ACCESSION   DATACA010000569 DATACA010000000
VERSION     DATACA010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642044"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VHK28_11355"
     CDS             <1..338
                     /locus_tag="VHK28_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142848.1"
                     /translation="SVAWGRWLPEREVATRGIVGVEVEPGAVRRLVAVADEHRMRVEP
                     MDLAPGEPMAAVSGVECLLEVRLQDGATFRISGPGAGGPVSVGATYADLGRLLAGERP
                     VLFAPRVAS"
     gene            335..1366
                     /locus_tag="VHK28_11360"
     CDS             335..1366
                     /locus_tag="VHK28_11360"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX2142849.1"
                     /translation="MTEGWPTVGVAGARGVVGGVFLSILADAGLPPACLRAFGSEPGT
                     VAYGDARVPVTVLADTTARDLDVLFLATDGESSRTIVAGPGRNVPLIVDNSSTFRLDP
                     AVPLVVPEANADALRGYDRNLVANPNCTTAAAVVALAPIRDLAGLEAVDLASYQAVSG
                     AGRAGLEAYEAERAAGGTDRAEGSPFHGRIADNVVPQIDVLDEEGWTGEERKIMAEVR
                     KILRLPDLDISATTVRVPVHTGHSVALHVRTQRDVDLREVMLALSNAPGVEYRHATGS
                     NRHPMPLDVVGRDPVLVGRLRAVPGRPRALALFASSDNLRKGAALNAIQIAAAADPDR
                     FGRLVPAPR"
     gene            complement(1556..>1882)
                     /locus_tag="VHK28_11365"
     CDS             complement(1556..>1882)
                     /locus_tag="VHK28_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142850.1"
                     /translation="WHGLVASLPAVTSVSVAWALPNSFFQLLTYAITAPFALGALLSG
                     LLPVDGTAPKWAAPLVAVVVGTAALLTTPFLALAGLVTGVTWWRLPARRQPRTAALHR
                     CERESR"
BASE COUNT          264 a          698 c          652 g          268 t
ORIGIN      
        1 cctcggtggc gtggggccgc tggctgccgg agcgagaggt tgccacccgc ggtatcgtcg
       61 gcgttgaggt cgagccaggc gccgtccgcc gcctggtggc cgtagccgat gaacacagga
      121 tgagggtgga acccatggat ctggctcccg gtgaaccgat ggcagccgtg tcgggcgtcg
      181 agtgcctgct cgaggtccgg ctgcaggacg gtgccacctt ccgcatctcc gggcccgggg
      241 ccggcggccc ggtcagcgtg ggtgccacct atgccgacct cggccgtctc ctggccggcg
      301 agcgtccggt gctcttcgca ccccgggtgg cctcgtgacc gagggctggc cgaccgtcgg
      361 cgtcgccggc gcccgcggcg tggtgggcgg cgtcttcctt tccatcctgg ccgatgcggg
      421 ccttccgccc gcctgcctcc gcgccttcgg ctccgagccg ggcacggtcg cctacggcga
      481 cgcgcgggtg ccggtcaccg tgctggccga tacgaccgcg cgcgatctgg acgtcctctt
      541 cctggcgacc gatggagagt cctcccgcac gatcgtggcg ggtccaggac gaaacgtccc
      601 gctcatcgtc gataactcga gcaccttccg gctcgatccg gccgtgccgc tggtcgttcc
      661 cgaggcgaac gccgacgcgt tgcgaggcta cgaccgcaac ctggtcgcca acccgaactg
      721 caccacggcc gcggccgtgg tcgcgctggc tcccatccgg gacctggccg gcctcgaggc
      781 ggtcgacctg gcctcgtacc aggcggtcag tggcgccggc cgcgccggcc tggaggccta
      841 cgaggcagag cgcgccgccg gcggcaccga ccgtgccgag ggctcgccgt tccatggacg
      901 gatcgcggac aacgtcgtgc cacagatcga cgtcctggac gaggagggct ggaccggcga
      961 ggagcgcaag atcatggccg aggtgcgcaa gatcctgcgt ctgccggacc tggacatctc
     1021 cgcgaccacc gtccgcgtcc cggtgcacac cggccactcg gtcgccctcc acgtccgcac
     1081 ccagcgggac gtggacctgc gcgaggtcat gctggcactg tcgaacgctc ccggcgtgga
     1141 gtaccgacat gccaccggct ccaaccgcca cccgatgccg ctcgacgtcg tcgggcgcga
     1201 cccggtgctg gtcggccgcc tgcgcgccgt cccggggcgg ccgcgggcgc tggccctgtt
     1261 cgcgtcctcc gacaacctgc gcaagggagc ggccctgaac gccatccaga tcgcagccgc
     1321 ggccgaccct gatcgattcg gccgcctggt gcccgccccc cgctgacccc gcgtctgttc
     1381 acgggtgcga atgtccgtca cgttatcgcg ccgaaccgct gccttcgccc ccagacgcga
     1441 ctggcggtcg cgttagagaa cagacagccc ccaaattggc caatttcgcg ccctccatcc
     1501 gcgccgcacg ctcaccggag tggctcgaca gtgagaacag gtgtgaacag cttctctagc
     1561 gcgattcccg ttcgcatctg tgaagagctg ccgtcctcgg ctggcgacgg gcagggagcc
     1621 gccaccaggt gacgccggtg accaggccgg ccagcgccag aaacggcgtg gtcagcaggg
     1681 cagcagtgcc caccaccacc gcgaccagcg gggccgccca tttgggcgcc gtgccgtcga
     1741 ccggcagcag gccggaaagg agggcgccga gcgcgaatgg cgcggtgatg gcgtacgtca
     1801 gcagctggaa gaaggaattc ggcagcgccc acgccacgga caccgacgtc accgctggca
     1861 agctcgccac cagcccgtgc ca
//
LOCUS       DATACA010000570         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2810371, whole genome shotgun sequence.
ACCESSION   DATACA010000570 DATACA010000000
VERSION     DATACA010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810371"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VHK28_11370"
     CDS             <1..243
                     /locus_tag="VHK28_11370"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEX2142851.1"
                     /translation="GVPMDGGALGGADALYSIVQMPPGVPVAAVAIGSPGARNAGHLA
                     ARILALADRDLAQRVADFREEQAGGAEVGIDPHAGS"
     gene            615..1928
                     /gene="purB"
                     /locus_tag="VHK28_11375"
     CDS             615..1928
                     /gene="purB"
                     /locus_tag="VHK28_11375"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX2142852.1"
                     /translation="MIDRYALPELRELFGERRRLELWLRIELLAVEALHDAGIVPTQD
                     WERIRQAATDVDPDRAREIEKESQHDVIAFLRSVTERLGPEGRWLHYGLTSSDVLDTA
                     TAVVLRDATGVVEGELQRLTQVVRRLALQHRSTPMVGRTHGIHAEPITFGFKVAGWYA
                     ELVRDAERLSRAREGISVGTISGAVGTHANVSAGVEEHVCAALGLAADPAATQVVGRD
                     RHAELLNALAVLGGTLERVAVEVRHLQRTEVGEAFEPFGSGQQGSSAMPHKRNPVLAE
                     RVSGLARLLRGDALIGLENMALWHERDISHSSAERFVFERSLGVAAYATRTLADVLDG
                     LEVDTERMAANLDRLGGMVYSEALLLAMVAKGADRQEAYRLVQGAAKRSWGGGASSFR
                     DEMLADEGVAEWLNAAEIERAMDLEHHLAGVEHTFRALGLDDGEA"
     gene            1937..>2245
                     /locus_tag="VHK28_11380"
     CDS             1937..>2245
                     /locus_tag="VHK28_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142853.1"
                     /translation="MLAWLRRGAYAAQLAGDRADLWPAGTLAWLAYLGWAPLLVVVSE
                     PDPRELQSIGVRLVTSSAFPANVIAAAGVLVGVVVLLAILAAAAEVALQRAAAAPSAS
                     R"
BASE COUNT          305 a          781 c          846 g          313 t
ORIGIN      
        1 ggcgtgccca tggacggtgg cgccctgggc ggcgcggacg ccctgtactc gatcgtgcag
       61 atgccgcccg gggtcccggt ggccgcggtg gcgatcgggt cgcccggggc ccggaatgcc
      121 ggacacctgg cggcgcgcat cctcgccctg gccgataggg acctggctca gcgagtggcc
      181 gattttcgcg aagagcaggc cggcggggcc gaggtgggga tcgacccgca cgcagggagc
      241 tgaggcgggc cagccctggg cccccggcgg cgccgccgtg gaggtgctcc ccaacttacg
      301 tcgcggcgct cgccgtgcat cagcggcatg gcaggaactc cacgatcgtg gcggcgaagt
      361 ggaatccggg aggctttcgt gcggaaatgc agcacgggtg cggcataagc gccacgacca
      421 ggcgtaacga gcgggacatg tcagcgttga ggcgtaccgg tacgacattt cccaaggaaa
      481 cgtcccccac aggaggccac gaacgaggtt cgtgaacgtt ctgcctcacg ggtgaggcac
      541 cggtcgagct gggacatccg cacggttcca cagcggcagc cgcgcgtccg ccgccgtcgc
      601 cctatccttc cggggtgatc gatcgctacg ccctgcccga gctgcgggag ctgttcggcg
      661 agcggcgccg gctggagctc tggctgcgga tcgagctgct ggccgtcgag gcgttgcacg
      721 acgcgggcat cgtccccacc caggactggg agcgcatccg acaggcggcc accgacgtcg
      781 accccgaccg cgccagggag atcgagaagg agtcgcagca cgacgtcatc gctttcctgc
      841 gatcggtgac cgagcggctc ggaccggagg gccgctggct gcactatggc ctgaccagct
      901 ccgacgtcct ggataccgcc accgccgtcg tcctgcgcga cgcgaccggc gtcgtggagg
      961 gtgagctgca acggctgacg caggtggtac ggcggctggc gctgcagcat cggtccacgc
     1021 cgatggtcgg gcgcacgcac ggcatccacg ccgaaccgat caccttcggc ttcaaggtcg
     1081 ccggctggta cgccgagctg gtgcgtgacg ccgagcggct gtcgcgcgct cgcgagggaa
     1141 tcagcgtcgg gaccatctcg ggcgcggtcg gcacccacgc caacgtcagc gcgggggtgg
     1201 aggagcatgt ctgtgccgcg ctaggcctgg ccgcggaccc ggctgccacc caggtcgtcg
     1261 gtcgcgatcg gcacgccgag ctgctgaacg cgctggccgt cctgggcggc acgctggaac
     1321 gggtcgcggt cgaggtgcgc cacctgcagc ggacggaggt cggggaggcc ttcgagccgt
     1381 tcggcagcgg gcagcagggc agcagcgcca tgccccacaa gcgcaacccg gtgctggccg
     1441 agcgagtgtc cggcctggcg cggctgctgc ggggtgacgc cctgatcggg ctcgagaaca
     1501 tggcgctctg gcacgagcgc gacatcagcc actcgtccgc cgagcgcttc gtcttcgagc
     1561 gctccctggg agtggctgcc tacgccaccc gcaccctcgc cgacgtcctg gacggcctgg
     1621 aggtcgacac cgagcgcatg gcggccaacc tggaccgcct gggcgggatg gtctactccg
     1681 aggctttgct gctggccatg gtggccaagg gggccgaccg ccaagaggcg taccgcctgg
     1741 tgcagggggc cgccaaacgc tcctggggcg ggggcgccag ctccttccgc gacgagatgc
     1801 tggccgacga gggcgtggcc gagtggctga acgccgccga gatcgagcgc gccatggatc
     1861 tcgagcacca cctcgccggc gtggagcaca ccttccgggc gctcggcctg gacgacggcg
     1921 aggcatagcc cggctcgtgc tcgcctggct gcgccgcggc gcctacgccg cccagctcgc
     1981 cggcgatcgt gccgacctct ggccggcggg cacgctggcc tggttggcct acctgggctg
     2041 ggcaccgctg ctggtcgtcg tgtccgagcc ggatccacgg gagctccagt cgatcggcgt
     2101 gcggctggtg acctcgagcg ccttcccggc caacgtcatc gccgcggccg gcgtcctggt
     2161 gggcgtcgtc gtcctgctgg ccatcctggc ggccgcggcc gaggtggcgc tgcagcgcgc
     2221 ggcggccgcg ccgtcggcct cgcgg
//
LOCUS       DATACA010000571         8316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_1766740, whole genome shotgun sequence.
ACCESSION   DATACA010000571 DATACA010000000
VERSION     DATACA010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766740"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1863)
                     /locus_tag="VHK28_11385"
     CDS             complement(157..1863)
                     /locus_tag="VHK28_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX2142854.1"
                     /translation="MGAERLKPALERAPERRHRFITQSGVEIDRVYTPADLYDPTNDP
                     DAGRPRYRSRATAEQAAGWDEIDDLGLPGEPPFTRGIHPTGHRGRLWTMRMFAGFGTA
                     EDTNGRFKKLIEAGGTGLSIAYDMPTLYGYDHDEPQAAGEFGTCGVAVSSLADMEILL
                     DGLPVGEISTSMTINSPAAMIWAMYIVAAEKMGVPRAQLSGTLQNDILKEFIAQKEYI
                     FPPAPSLKLVTDTVEWGTRELPRWNTISISGYHIREAGSTAVQELAFTIADGIAYVED
                     ALSRGLRFDDFAPRLSFFFNSHNDFFEEIAKFRAARRIWYKLSTERFGAENERSTWMR
                     FHTQTAGVSLTAQQPLNNLTRVAVQALAGVLGGTQSLHTDAYDEAWAVPSEEAALVAL
                     RQQQILAEESGVANTVDPLAGSYFVETLTNRTEQAAWRYIAQIDEMGGMVKAIEAGYP
                     QAEIADAAYEQSREVEAHEREIVGVSRHVNPNEELRIPLLEISREQERRHLDRLARVR
                     AERDADAHARAMTRLREEAAQGDTNLMPAIVEAVQAYATIGEMCNALRAVYGEYREPL
                     AV"
     gene            complement(1949..2887)
                     /locus_tag="VHK28_11390"
     CDS             complement(1949..2887)
                     /locus_tag="VHK28_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX2142855.1"
                     /translation="MTTPAFAAPKDLLTELEPVAERLLNRHLQMTKEWFPHDFIPYSL
                     GRDYDKEPWTPDQPRISGVAQIAFEVNLLTEDNLPSYHREIHRMFGRGDGAWINWVNR
                     WTAEEGRHGIVLRDYLVVTRNIDPYSLERSRMAQLQTAYSHDDGGNVLRGIAYVAFQE
                     LATRISHRNTGRYSSDPVADRIMARIAADENLHMVFYRDLLTEALEIDPSAAVHAIAD
                     EIIDFQMPGAGMENFTRKAAQMAKAGIYDLRVHHDEVVWPLLNHWGVFELDNLDASAR
                     LRREELRRFLTDLDAQASRFEAKRALLFEREAARAG"
     gene            3003..3575
                     /locus_tag="VHK28_11395"
     CDS             3003..3575
                     /locus_tag="VHK28_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142856.1"
                     /translation="MQRNGFTLRAIGIGCALLLGLVACDPESTPGEGGEPTPTPQVEA
                     TLIPAQEAVAAIEALEGGFTCDEPRPRGGDDFWWLCSGSRGTLDAEIRVYARQVDDPL
                     HAVTMYTSVPQEEEAEQAADATSELILAVVGVTVPEEWRGSVSEWVEGVMPDGGRTLD
                     VPDSGVTAGIQPLTANEWYVEFYELDGTPG"
     gene            complement(3572..4843)
                     /locus_tag="VHK28_11400"
     CDS             complement(3572..4843)
                     /locus_tag="VHK28_11400"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2142857.1"
                     /translation="MDDRTTTQSPRDDVWGAAQRAVDLAAERLDLDPGMHQVLRVPKR
                     ELTVHFPVSMDGGGVEVFTGYRVQHNLNRGPATGGIRFTEDLTLDLVRANAMANTWKA
                     ALVQIPYGGSMGGVVVNPRRLSEHERKGLTRRFATEISVLIGPDRDIPTPDVNTGSQT
                     MAWIMDTFSMHRGHTVPAVVTGKPQAVGGTRGRREATSRGAFRCILAAARARNLRLDG
                     ATVALQGFGRVGSVLAEMLVGAGARVIAIADDQHAVSNPKGIGVGQAIEWARAHDSVE
                     GVPETEPMPRAELFALDCDILVSAGLQYQITAEVAAGVRAGILVEAGNSPTTPEADVT
                     LADRDIVVLPDILCTAGGLVLAYFEWVQDMQAFFWSEQEVVAELERIMDDAVGSVMAM
                     SQARSVDLRGAAMMVAVQRVAEATTLRGLYP"
     gene            complement(4844..6793)
                     /locus_tag="VHK28_11405"
     CDS             complement(4844..6793)
                     /locus_tag="VHK28_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX2142858.1"
                     /translation="MPDLFNLVGAGPLLVIVAVIIVALLASYVASRYRVANANEALIV
                     AGSRGAKVRDERGEVAAAAERADKGIKVVVGGGTFVLPLIHKVGRLKLTARQIGVALP
                     DAVTSQGIKVAVQGVATFKIGRDVESIRNAAERFLDAKDEQVDSIVKNVLEGSLRSIV
                     GTLTIEELIMDRQKLLQQVQDSAKGDLATSGLQIDAFTIQSINDESGYVDLLGQQKLA
                     IVERDARVAKAGADQEAAVREAEAQQIKINAGRDVALREAEVQTQTAAAQARAAQSGP
                     LAQAEAQQEVTRRQTELADLEAARKEKELLASTVRPAEAEAEAVVRRADGDRQAKIAA
                     AQAEAERAKLAGQAEADVDVAKGEAQARVVRVNAEAEALRVTAEGNAEAEVTLTKGEA
                     EAKALALRAEAFRLFNDAAIIQTVLGQLPDIVRAAAEPMASIDSLTVLSSDGASDVVR
                     TTTRTVAEASAAVKGLTGLDVPDLIGRAMSAQVAGEGAPVPGTVTTAAGPDVPPTTTP
                     RRGRRPGSAARSTPPSPATPPTNGEGEPAPRMPAAAAAPTLEEPMEAMRRAATSIPAV
                     GTPAQVDLEIPDSAQADEVLARLGEALAAVPGIGRYRDSRLEDLAERGPRLARGLWNR
                     FGARVDPAYRELTVGDIIDRAGSAG"
     gene            complement(6786..7337)
                     /locus_tag="VHK28_11410"
     CDS             complement(6786..7337)
                     /locus_tag="VHK28_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX2142859.1"
                     /translation="MFTIDVANTIFVVCLLAGGILLLVTVLLGDLLGGLLDSFNLDFD
                     LGGVSLMPLLLAFVAMFGVGGLIGTEALDMDAGRASLVGAGFGALGAGIVFVLFNLLK
                     RAEAPPAFSLEDLVGRSGRVSVSIPAGRNGSVLLSYGGASHDLTATADREISAGTLVS
                     VTDVVGGTLIVAPLTTSGGEIDA"
     gene            7518..>8316
                     /locus_tag="VHK28_11415"
     CDS             7518..>8316
                     /locus_tag="VHK28_11415"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142860.1"
                     /translation="MAPRVMTVGGPIPPERIGFTLIHEHTSSSPEVARTRVELLDYDT
                     DFELMVSELRDFRRRGGSCLVDATTVGIGRDPARLRSLSTSSNLYLVMGAGWYRESMY
                     PASADIDGRTADDLAAEIEAEFREGVAGTGVRPGIIGEIGTDRARVSAREERVHRAVA
                     RAARLTGLAILTHSLHSRVGLEQLRIFEEEGADPSRVVIGHADSLPDLDYHLAILERG
                     ASLGFDLLGRSGDYAAARETQLVQIILELLERGFADRILLSHDTCTNV"
BASE COUNT         1151 a         2924 c         2949 g         1292 t
ORIGIN      
        1 cattcaccgg ccgcccgtcc ggcgcgcgct gcgccggacg cgagtagtag gcgcggaaga
       61 gctcgaaggt ggcgtccacc aggtgcagct tcatcgctgt cgaggataga cgtcgaccgc
      121 tcatgcggcg tcccgcctga ccgatggagc agctcgtcag acggcgagcg gctcccggta
      181 ctcgccgtag acggcgcgca gggcgttgca catctcgccg atggtcgcgt aggcctgcac
      241 ggcctccacg atggccggca tcaggttggt gtcgccctgc gccgcctcct cgcgcaggcg
      301 ggtcatggcg cgagcgtggg cgtccgcgtc gcgctccgcg cggacgcggg ccaaccggtc
      361 caggtgccgc cgctcctgct cgcggctgat ctcgagcagc gggatgcgca gctcctcgtt
      421 cgggttgacg tggcggctga cgcccacgat ctcgcgctca tgcgcctcca cctcgcgcga
      481 ctgctcgtag gcggcatcgg cgatctcggc ctgcgggtag ccggcctcga tggccttgac
      541 catgccgccc atctcgtcga tctgggcgat gtagcgccag gcggcctgct cggtccggtt
      601 ggtcagcgtc tcgacgaagt aggagccggc aagcgggtcg acggtgttgg ccaccccgga
      661 ctcctcggcc aggatctgct gctgccgcag ggcgaccagg gcggcctcct cggagggaac
      721 tgcccacgcc tcgtcgtacg catcggtatg gaggctctgg gtcccgccca gcaccccggc
      781 cagcgcctgc accgccaccc gggtcaggtt gttgagcggc tgctgcgcgg tcagcgacac
      841 gccggcggtc tgggtgtgga agcgcatcca ggtgctgcgc tcattctcgg cgccgaagcg
      901 ctcggtgctg agcttgtacc agatgcggcg cgcggcacgg aacttggcga tctcctcgaa
      961 gaagtcgttg tggctgttga agaagaagct gaggcgcggc gcgaagtcgt cgaagcgcag
     1021 gccgcgtgac agcgcgtcct ctacgtaggc gatgccgtcg gcgatggtga aggccagctc
     1081 ctggacggcg gtcgagcccg cttcccggat gtggtaccca ctgatgctga tcgtgttcca
     1141 gcggggcagt tcgcgggtgc cccactccac ggtatcggtc accagcttca acgagggtgc
     1201 cggcgggaag atgtactcct tctgggcgat gaactccttg aggatgtcgt tctgcagcgt
     1261 cccggacagc tgcgcgcgcg gcacgcccat cttctcggcg gccacgatgt acatggccca
     1321 gatcatggcc gccggcgagt tgatggtcat cgaggtgctg atctcgccga ccggcaggcc
     1381 gtcgagcagg atctccatgt cggccaggct ggacaccgcc accccgcagg tgccgaactc
     1441 gccggccgcc tgcggctcgt cgtggtcgta gccgtagagg gttggcatgt cgtaggcgat
     1501 cgacagcccc gtaccgccgg cctcgatcag cttcttgaag cgcccgttgg tgtcctccgc
     1561 ggttccgaag ccggcgaaca tgcgcatggt ccacagacgc ccgcggtggc cggtggggtg
     1621 gatgccgcgc gtgaagggcg gctcaccggg caggcccagg tcgtcgatct cgtcccagcc
     1681 ggcggcctgc tcggccgtgg cgcgcgaccg gtagcgtggc cgccccgcgt cgggatcgtt
     1741 ggtggggtcg tagaggtccg ccggcgtgta gacccggtcg atctccaccc ccgactgggt
     1801 gatgaaccga tggcgccgct ccggcgcccg ctccagcgcg ggcttcagcc gttcggcccc
     1861 catcggtcct ggtgctcccg ccagcgcttc atgtcagtca ccccgcaagt gtgcgggcaa
     1921 gaccgatgcc cccgcaaccg gccgtggact agccggcgcg cgccgcctcg cgctcgaaga
     1981 ggagggcgcg cttcgcctcg aatcggctgg cctgcgcgtc gagatcggtc aggaagcggc
     2041 gaagctcctc gcggcgcagc cgggcggatg cgtccaggtt gtcgagctcg aagacgcccc
     2101 agtggttgag gagcggccag acgacctcgt cgtggtgaac gcgcaggtcg tagatgccgg
     2161 ccttcgccat ctgcgccgcc ttgcgggtga agttctccat cccggcgccg ggcatctgga
     2221 agtcgatgat ctcgtcggcg atggcgtgca ccgcggcgga aggatcgatc tccagcgcct
     2281 ccgtgagcag gtcgcggtag aagaccatgt gcaggttctc gtcggccgcg atgcgcgcca
     2341 tgatgcggtc ggcgaccgga tccgaggagt agcggccggt gttgcggtgg ctgatccggg
     2401 tggccagctc ctggaaggcg acgtaggcga tgccgcggag cacgttgccg ccgtcgtcat
     2461 ggctgtacgc ggtctgcagc tgggccatgc gtgagcgttc cagggagtag ggatcgatgt
     2521 tgcgcgtgac caccaggtag tcgcgcagca cgatgccgtg gcgcccctcc tccgcggtcc
     2581 atcggttcac ccagttgatc caggcgccgt cgccccggcc gaacatgcga tggatctcgc
     2641 ggtggtagct cggcaggttg tcctcggtga gcaggttcac ctcgaacgcg atctgcgcca
     2701 cgccggagat gcgcggctgg tccggggtcc acggctcctt gtcgtaatcg cgtcccagcg
     2761 agtacgggat gaagtcgtgc gggaaccact ccttggtcat ctgcaggtgg cggttcagca
     2821 ggcgctcggc cacgggctcg agctcggtga gcaggtcctt gggagccgca aaggcggggg
     2881 tcgtcatggg gtcgtcctcg gtgaagcgca ccggcttgac acacgacgcg cgagatgtga
     2941 aggctcatgg tcgcacgaat tgggtccgag cgggtggcac gacccttgcg gccgggcccg
     3001 gcatgcagcg caacggcttc acactccgcg cgatcgggat cggatgcgca ttgctgctgg
     3061 ggctggttgc ctgcgacccg gagagcacgc ctggcgaggg cggcgaacct actcccacgc
     3121 cgcaggtcga ggctaccctg attccggccc aggaggcggt ggccgcaatc gaggcgctcg
     3181 agggaggctt cacctgcgac gagccgcgtc cccgcggcgg cgacgacttc tggtggctgt
     3241 gcagcggcag ccgcggcacg ctggacgccg agatccgggt ctacgcgagg caggtggatg
     3301 acccgctcca cgccgtgacc atgtacacgt ccgtcccgca ggaggaggag gctgagcagg
     3361 ccgccgacgc cacgtcggag ctgatcctgg cggtcgtggg cgtgaccgtt ccggaggagt
     3421 ggcgtggctc cgtgtcggag tgggtggagg gtgtcatgcc ggacggcgga cgcaccctgg
     3481 acgtgccaga cagtggcgtg accgcgggca tccagccgct gaccgccaac gagtggtacg
     3541 tcgagttcta cgagctcgac gggacgccgg gttaggggta cagcccgcgc agggtggttg
     3601 cctctgccac gcgctggacg gccaccatca tcgccgcccc gcgcaggtcc accgagcgcg
     3661 cctggctcat ggccatcacg ctgccgaccg cgtcgtccat gatgcgctcc agctcggcga
     3721 ccacctcctg ctcgctccag aagaatgcct gcatgtcctg cacccactcg aagtaggcga
     3781 ggaccaggcc cccagcggtg cacaggatgt caggcagcac cacgatgtcc cggtcggcaa
     3841 gcgtcacgtc cgcctcgggg gtcgtgggcg agttgcccgc ttcgaccagg atcccggcgc
     3901 gcactccggc ggccacctcg gcggtgatct ggtactgcag gccggccgac acgaggatgt
     3961 cgcagtcgag cgcgaacagc tcggcacgag gcatcggctc cgtctccggc acgccctcca
     4021 cgctgtcgtg cgcgcgggcc cattcgatcg cctggccgac cccgatgccc ttggggttgc
     4081 tgaccgcatg ctggtcgtcg gcgatggcga tcacccgcgc gccggcgccc accagcatct
     4141 cggccagcac gctcccaacc cggccgaagc cctgcaacgc caccgtggcc ccatccagcc
     4201 gcaggttgcg ggcgcgggcg gcggccagga tgcagcggaa ggcgccgcgg ctcgtcgcct
     4261 cgcgccggcc gcgggtgccc cccaccgcct gcggcttgcc ggtgaccacg gccggcacgg
     4321 tgtgcccgcg atgcatgctg aaggtgtcca tgatccaggc catggtctgc gacccggtgt
     4381 tgacgtccgg ggtcgggatg tcccggtctg gaccgatgag aacgctgatc tcggtggcaa
     4441 agcggcgggt caggcccttg cgctcgtgct cgctgagacg acgcggattc accaccacgc
     4501 cgcccatgga gccgccatag gggatctgca ccagcgccgc cttccaggtg ttggccatgg
     4561 cgttggcgcg caccaggtcc agggtcaggt cctcggtgaa gcgaatgcca ccggtcgccg
     4621 ggccccggtt gaggttgtgc tggacgcggt agccggtgaa cacctcgacg ccgccgccgt
     4681 ccatgctgac cgggaagtga acggtcagct cccgcttcgg cacgcgcagc acctggtgca
     4741 tgccgggatc gaggtcgagc cgctcggcgg cgaggtccac cgcgcgctgg gcggcgcccc
     4801 acacgtcgtc gcgtggtgac tgggtggtgg tgcgatcgtc catctagccc gcgctcccgg
     4861 cacggtcgat gatgtcgccg accgtcagct cgcgatacgc cgggtcgacc cgcgcgccga
     4921 accggttcca cagcccgcgt gccaggcgcg ggccgcgctc cgcgaggtcc tccagccgcg
     4981 agtcccggta gcggccgatg cccggcacgg cggcgagcgc ctcgcccagg cgtgcgagca
     5041 cctcgtccgc ctgggcagaa tcggggatct cgaggtccac ctgcgccggg gtgccaacgg
     5101 cggggatgct cgtggccgcg cggcgcattg cctccatcgg ctcctcgagc gtgggggccg
     5161 ccgctgcggc gggcatgcgc ggagccggct caccctctcc gttggtgggg ggtgttgcag
     5221 gggacggtgg agtcgaccga gcggccgagc cgggacgacg tcctcgacgc ggcgtcgtcg
     5281 tggggggcac atccgggcca gcggccgtgg tgacggtgcc cggcaccggt gcgccctcgc
     5341 cggccacctg cgcgctcatg gccctgccga tgagatccgg cacgtccagg ccggtgaggc
     5401 ccttcaccgc agcgctcgct tcggccaccg tgcgggtggt ggtgcgcacc acgtcggacg
     5461 ccccgtcgct ggagagcacg gtgagcgagt cgatgctggc catcggctcc gcggccgcac
     5521 gaacgatgtc cggcagctgg ccgaggacgg tctggatgat ggccgcgtcg ttgaagaggc
     5581 ggaacgcctc ggcccgcagg gccagcgcct tggcctcggc ctcgcccttg gtgagggtca
     5641 cctccgcctc cgcgttgccc tccgccgtga cccgcagcgc ctcggcctcc gcattgaccc
     5701 gcaccacgcg ggcctgcgcc tcgcccttgg cgacgtccac gtccgcctcc gcctgtccgg
     5761 cgagcttggc gcgctccgcc tcggcctggg ccgcggcgat ctttgcctgc cggtcgccgt
     5821 ccgcgcgtcg aacgaccgcc tcggcctccg cctcggcggg ccggacggtg gaggccagca
     5881 gctccttctc cttgcgtgcc gcctcgagat cggcgagctc ggtctggcgg cgagtgacct
     5941 cctgctgcgc ctcggcctgg gccagcggcc cggactgggc ggcgcgtgcc tgggcggccg
     6001 ccgtctgggt ctggacctcc gcctcgcgca gggcgacgtc ccgcccggcg ttgatcttga
     6061 tctgctgcgc ctccgcctcg cgaaccgcgg cctcctggtc ggcaccggcc ttggccacgc
     6121 gcgcatcgcg ctccacgatc gccagcttct gctggccgag caggtcgacg tagccggact
     6181 cgtcgttgat cgactgaatg gtgaaagcgt cgatctgcag cccggaggtg gccaggtcgc
     6241 ccttggccga gtcctggacc tgctggagga gcttctggcg gtccatgatc agctcctcga
     6301 tcgtcagcgt gccgacgatc gagcgcagcg atccctccag cacgttcttg acgatggagt
     6361 cgacctgctc gtccttggcg tccaggaagc gttcggccgc gttgcggatg gattccacgt
     6421 cgcggccgat cttgaaggtg gcgacgccct gcaccgccac cttgatgccc tggctcgtga
     6481 ccgcgtccgg cagtgccacg ccgatctggc gagctgtcag cttcaggcgc ccgaccttgt
     6541 ggatgagcgg cagcacgaac gtgccgccgc ccaccacgac cttgatgccc ttgtcggcgc
     6601 gctcggcggc ggcggccacc tcgccgcgct cgtcgcgcac cttggcgccg cgcgagccgg
     6661 ccacgatgag ggcctcgttg gcgttggcga cccggtagcg gctggcaacg tagctggcca
     6721 gcagggccac gatgatgacg gcgacgatca cgagcagcgg gccggcgccc accaggttga
     6781 acaggtcagg catcgatctc ccctcctgag gtggtgagcg gcgccacgat gagcgtgccg
     6841 ccgacgacat cggtcaccga gaccagggtg ccggccgaga tctcgcggtc agcggtcgca
     6901 gtgaggtcat gtgaagctcc gccgtagctg aggagcacgc tcccgttacg gccggccgga
     6961 atgctgacgc tgacccggcc cgaacgcccg accaggtcct ccaggctgaa cgccggcggc
     7021 gcctcggccc gcttcagcag gttgaagagc acgaacacga tcccggcgcc cagcgcgccg
     7081 aagccggcgc ccaccagcga ggcgcgaccg gcatccatgt ccagcgcttc ggtgccgatc
     7141 agcccgccga cgccgaacat ggccacgaag gccagcagca gcggcatcag cgacacgccg
     7201 ccgaggtcga agtcgaggtt gaacgagtcc agcaggccgc ccagcaggtc gcccagcagc
     7261 accgtcacca gcagcaggat gccgcccgcc agcaggcaca ccacgaagat ggtgttggcg
     7321 acgtcgatgg tgaacacccg atccatccct ccgtccgcct gccggctggg gcgggcgggc
     7381 gacgaggata cccttgcccg ctcgcccccg acaaggactt cggccgggat ggcacacttg
     7441 cacggcctac ctcaccctcg acgccggcgg ctccaaccgc tcctgaccgg cgccgacccg
     7501 cacatccgga ggctgccatg gcgccacgcg tgatgaccgt cggtggaccg ataccccccg
     7561 agcggatcgg gttcaccctc atccatgagc acacctcgtc gagccccgag gtggcgcgca
     7621 ccagggtgga gctgctcgac tacgacaccg acttcgagct gatggtatcc gagctgcgtg
     7681 acttccgtcg ccgcggcggc agctgcctgg tggacgccac cacggtcggc atcggtcggg
     7741 atccggcccg cctccgcagc ctgtccacca gctccaacct ctacctggtc atgggtgccg
     7801 gctggtaccg cgagagcatg taccccgcca gcgccgacat cgatggacgg acggccgatg
     7861 acctggccgc cgagatcgag gccgagtttc gcgagggcgt ggccgggacc ggcgtgcggc
     7921 ccggcatcat cggcgagatc gggaccgatc gggcccgggt cagcgcccgc gaggagcgcg
     7981 tgcaccgggc tgtggcccgc gccgcccgcc tcaccggcct ggcgatcctt acccactcgc
     8041 tgcacagccg ggttgggctg gagcagctgc gcatcttcga ggaggagggc gccgacccct
     8101 cgcgggtggt catcggtcat gccgactcgt tgccggacct cgactaccac ctggccatcc
     8161 tggaacgggg agcgagcctg ggcttcgatt tgctgggccg ctccggcgac tacgcggcgg
     8221 cgcgcgagac gcagctggtg cagatcatcc tggagctgtt ggagcgcggc ttcgccgatc
     8281 ggatcctgct cagccacgac acctgcacca acgtcc
//
LOCUS       DATACA010000572         3302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindria bacterium isolate SMAG_U6712
            k141_2811065, whole genome shotgun sequence.
ACCESSION   DATACA010000572 DATACA010000000
VERSION     DATACA010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955334
            Sequence Read Archive: SRR12676706
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindria bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindria bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 01:57:03
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,289
            CDSs (total)                      :: 2,254
            Genes (coding)                    :: 2,222
            CDSs (with protein)               :: 2,222
            Genes (RNA)                       :: 35
            tRNAs                             :: 33
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 32
            CDSs (without protein)            :: 32
            Pseudo Genes (ambiguous residues) :: 0 of 32
            Pseudo Genes (frameshifted)       :: 6 of 32
            Pseudo Genes (incomplete)         :: 24 of 32
            Pseudo Genes (internal stop)      :: 3 of 32
            Pseudo Genes (multiple problems)  :: 1 of 32
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Limnocylindria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811065"
                     /isolate="SMAG_U6712"
                     /isolation_source="soil"
                     /db_xref="taxon:3047546"
                     /environmental_sample
                     /country="USA: Arizona"
                     /lat_lon="34.05 N 114.3 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..132
                     /locus_tag="VHK28_11420"
     tRNA            45..132
                     /locus_tag="VHK28_11420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Leu,seq:taa)
     gene            complement(148..1383)
                     /locus_tag="VHK28_11425"
     CDS             complement(148..1383)
                     /locus_tag="VHK28_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2142861.1"
                     /translation="MPNPILICAPTDELRAELESLLGNVGYATHSVTTPADAVATLRD
                     APIDLVVAEGLAVTGAIGRIRTAAPVPTPILVVAPAGDVEARIAFLEAGADDVITTGF
                     ARHELEARVEALLIRAGRLRPETGRGGMPAGEIVTFLSPKGGVGTTTLAINTAILLAG
                     GSTIGGSTGGRADTSSPSQPTARVLLVDLDLQFGQVTTHLNVTPRFDIAGLAGDDQAL
                     NDPELALAYLTPHTSGVQVLAAPTHPDADFRVTAEQLDQAIAILRPKFDYVVVDCGSR
                     LDPRTLTILEQASIHVFIIFPEIAALRATSLLLGFLAETATLRARSLFVVNHIFPKEL
                     LKTRDIENLLRSKPAAEIPYTEVEMIRAVNEGVPLVLGRPGSPAAMAIHRVAQAVVGI
                     PTEAASASEGAKRRGLFRR"
     gene            1580..2164
                     /gene="rpsF"
                     /locus_tag="VHK28_11430"
     CDS             1580..2164
                     /gene="rpsF"
                     /locus_tag="VHK28_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX2142862.1"
                     /translation="MRRYELMLLIRPDLEDDKLQSAVEKVTRAIVNGGGALTKVSPWG
                     KRRMAYPIQTFRDASYFLIHFDIAPSAVREIERGLLISEEILRHLVVVIEDRGPLAEE
                     DLAPAVPAASSEEADGREPEAAAASAQQPEPAGEASDSPAEESAPGAEETEPAAGLPM
                     EADASEPAEPASEGDAGDAGEGGGEPEEAEAARA"
     gene            2192..2656
                     /gene="ssb"
                     /locus_tag="VHK28_11435"
     CDS             2192..2656
                     /gene="ssb"
                     /locus_tag="VHK28_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX2142863.1"
                     /translation="MTLNKMMIIGNLGADPELRYTPSGKAVAELRVAVNDRSRGPDGE
                     WQEETQWFRVELWEQAAERAAERLRKGHKVFAEGALRAREWEGKDGQKRTSLEIRFAR
                     VQSLERRDPSEGGFSGGSFGESGPSDAPVARPRGASQRPASEETIDVDDIPF"
     gene            2677..2946
                     /gene="rpsR"
                     /locus_tag="VHK28_11440"
     CDS             2677..2946
                     /gene="rpsR"
                     /locus_tag="VHK28_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX2142864.1"
                     /translation="MPSARSRRETADYYRDRRRRKVCNFCVDKAESIDYKEVNRLRRY
                     LSERAKIEPRRKTGTCARHQRMLTVALKRARHVALLPYAPQHLRP"
     gene            3025..>3302
                     /locus_tag="VHK28_11445"
     CDS             3025..>3302
                     /locus_tag="VHK28_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX2142865.1"
                     /translation="MDVVLLIGSLAIILVAAELFTNGIEWFGHKLNLAEGAVGSVLAA
                     VATAMPETLIPVIAILGPLIFGGDAEASHAIGVGAILGAPFMLSTLAM"
BASE COUNT          514 a         1108 c         1192 g          488 t
ORIGIN      
        1 gctagggagg cgttcctccg ctaccatcgt ggccgcagcc tgcggccgga gtggcgaaac
       61 ggcagacgcg gccggcttaa acccggcggc cccgaaaggg gcgtgggggt tcgaatccct
      121 cctccggcac caatctcagg cggtccgtca gcggcggaac aggccccggc gcttggcgcc
      181 ttcactggcc gacgcagcct cggtcgggat gccgaccacg gcttgggcca cgcgatggat
      241 tgccatcgcc gcgggcgatc ccggacggcc gagcaccagc ggcacgccct cgttgacggc
      301 ccggatcatc tccacttccg tataggggat ctcggccgcc ggcttggagc gcagcaggtt
      361 ctcgatgtcg cgcgtcttca gcagctcctt ggggaagatg tggttcacca cgaagaggct
      421 gcgcgcgcgg agggtggcag tctcggccag gaagccgagc agcaggctgg tggctcgcag
      481 cgccgcgatc tctggaaaga tgatgaagac gtggatgctg gcctgctcca ggatcgtgag
      541 ggtccggggg tcgagtcgcg acccgcagtc gaccacgacg tagtcgaact tgggccgcaa
      601 gatggcgatc gcctggtcca gctgctccgc ggtgacccgg aagtcggcat cgggatgggt
      661 gggcgcggcc agcacctgga cgccggaggt atgtggcgtc aggtaggcca gcgccagctc
      721 aggatcgttg agtgcctgat cgtcgccggc cagcccggcg atatcgaatc ggggcgtcac
      781 gttgaggtgt gtcgtcacct ggccgaactg caggtcgagg tccaccagga gcacgcgggc
      841 cgttggctgc gacggcgatg aggtatctgc acgcccgccg gtggatccgc cgatggtcga
      901 tccgccggcc agcaggatgg cggtgttgat cgccagagtg gtggtaccca cgcccccctt
      961 cggcgacagg aaggtcacga tctcgccggc cggcatgccc ccgcggcccg tctcagggcg
     1021 caggcggccc gcgcggatga gcagcgcctc gacccgtgcc tccagctcgt ggcgggcgaa
     1081 gccggtggtg atcacgtcgt ccgcgccggc ctccaggaag gcgatgcggg cctccacgtc
     1141 accggcgggt gcgaccacca ggatgggcgt aggcaccggc gccgcggttc ggatgcgtcc
     1201 gatggcgccg gtcaccgcaa ggccctcggc cacgaccagg tcgatcggcg cgtcgcgcag
     1261 ggtcgcgacc gcatcggcgg gcgtggtcac gctgtgcgtg gcgtacccaa cgttcccgag
     1321 gagtgattcc agctcggccc ggagctcgtc ggtcggtgcg cagatcagga tcgggttcgg
     1381 catcgtctcc agggccgtgg gctgggttgg tggccgagag tatacgaggt ggcgattgcc
     1441 agtccgtttc catcggtcta tcattcttgg gcgcctccgt ctgcggaggt gcgtttcatg
     1501 tcatttgggc tcctttctgc cgccttccgg agcgccagct tcggagcgcc gcaagcccat
     1561 agagaggagg agtcggctca tgcgccgata cgagctcatg ctcctgatcc gccccgacct
     1621 ggaggacgac aagctccagt ccgcggtgga gaaggtcacg cgcgccatcg tcaacggtgg
     1681 cggcgcactc accaaggtct cgccatgggg caagcgccgc atggcgtatc ccatccagac
     1741 gttccgcgat gcgagctact tcctgatcca cttcgacatc gcgccgtcgg ccgtgcgcga
     1801 gatcgagcgt ggcctgctga tcagcgagga gatcctgcgc catttggtgg tggtcatcga
     1861 ggaccgcggg cccctggccg aggaggacct cgcgccggcc gtgccggctg cgtcgtccga
     1921 ggaggcagac ggccgcgagc cggaggccgc cgccgcatcg gcgcagcaac cggagccggc
     1981 gggcgaggcc agcgattcac ctgccgagga gtccgcgccc ggcgcggagg agacggagcc
     2041 cgctgccggg ctgccaatgg aggcggacgc gtccgagccc gccgagcctg cgtccgaagg
     2101 agacgcgggt gatgccggtg agggcggcgg agaacctgag gaggccgagg cggcgcgcgc
     2161 ctgagcgcgg catcgagagg agagctgagc catgaccctc aacaagatga tgatcatcgg
     2221 caacctggga gctgaccccg agctgcgcta cacgcccagc ggcaaggcgg tcgccgagct
     2281 tcgggtggcg gtcaacgatc ggagccgcgg cccggacggc gagtggcagg aggagaccca
     2341 gtggttccgg gtggagctct gggagcaggc cgccgaacgc gcagccgagc gcctgcgcaa
     2401 gggccacaag gtcttcgccg agggcgccct gcgcgcccgc gagtgggagg gcaaggacgg
     2461 ccagaagcgc acctcgctcg agatccgctt cgcccgcgtc cagtccctgg agcgccggga
     2521 cccgtcggag ggcggcttca gcggcggcag ctttggcgag agcggaccat cggatgcgcc
     2581 ggttgctcgg ccgcggggcg ccagccagcg cccggcctcc gaggagacca tcgacgtcga
     2641 cgacatcccc ttctgaaacc gaggaacgag aaccgaatgc caagcgcacg atcccgccgc
     2701 gagacggcgg actactaccg agaccggcgt cgacgcaagg tctgcaactt ctgcgtcgac
     2761 aaggccgagt cgatcgacta caaggaggtc aaccgcctcc gacgctacct ctccgagcgg
     2821 gcgaagatcg agccgcgccg caagaccggc acctgcgccc gccaccagcg catgctgacc
     2881 gtggccctca agcgggcacg gcacgtggcc ctgcttccct acgcgcccca gcacctgcgc
     2941 ccgtaggcgc caccctctag acttccgccc tgataaccca cgccgcggcc cgttcgggcc
     3001 tcggccattg ctgaggctga gtccgtggac gtcgtcctgc tcatcggctc gttggcgatc
     3061 atcctggtcg ccgccgagct gttcaccaac ggcatcgagt ggttcggcca caagctgaac
     3121 ctggcggagg gggcggtggg cagcgtgctg gccgcggtgg cgacggcgat gccggagacc
     3181 ctgatcccgg tcatcgccat cctcggtccg ctgatcttcg gcggcgatgc ggaggcttcg
     3241 cacgccatcg gtgtgggcgc catcctgggc gcaccgttca tgctctcgac cctggccatg
     3301 tt
//
