LOCUS       DATAFW010000001         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_704926, whole genome shotgun sequence.
ACCESSION   DATAFW010000001 DATAFW010000000
VERSION     DATAFW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704926"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..686
                     /locus_tag="VHJ57_00005"
     CDS             519..686
                     /locus_tag="VHJ57_00005"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HEX2486950.1"
                     /translation="MPITDATKKQIAQARRLFYKICFKCGGRNPISSERCRKCHSQKM
                     RLKNRNIGAKK"
     gene            complement(731..2455)
                     /locus_tag="VHJ57_00010"
     CDS             complement(731..2455)
                     /locus_tag="VHJ57_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2486951.1"
                     /translation="MKSPKVVLSADRTLMSPYRGISLASFFGCAPALDFNRTKDSFWY
                     YILKNQVTPRVLFDFICNPIGNENGIANFAPYGLRKLEASLLRDGYNPNDVVVAHPDY
                     IEKFIGPETEVVGTYEMDPLGMGPVTMTFTFGRKTMSYDELYNRELHLRINTAKAKNG
                     SKAKVIVGASGTWQYNYDPAKIEEYGIYGIVEGDLGGIGPELDGVGARFFNAVINGDL
                     ETSNPFKKSEFKVEIKEFAKNDRKYHGRFFHYSDEPSIDEIPNIVNPSMHGMIEVMRG
                     CGRGCKFCDVTLRPLRYYPVEKVQKEIEINMKYGGLKNAWIQSDDIFVYGLNPRTTKN
                     MQPNREAIEELFRGIMDTGIEHTNPTHGTLAGAIADERLVPNISKIIKSGPDNFIGIQ
                     CGLETGSIRLIGKYADRKLAPFKPAEWHWVVKEGVKTLNEHYWVPAFTLIMGLDNNES
                     IDDSWETIRLIHELETEQPDSKFTVTPLTFVPIGLLEKSSFFDIGNTMDPGKLGVMYK
                     TWQHNFKHGIQKFMSKVGRHNTIKNTFFSGLAKTLGGIPLTSMEKFARSKGPEHEKAI
                     EKIKANYW"
     gene            2611..2802
                     /locus_tag="VHJ57_00015"
     CDS             2611..2802
                     /locus_tag="VHJ57_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486952.1"
                     /translation="MEEKIYCNICDVSIYVNEIENHITNSIHKKSKNEYIQQLLSFVE
                     RDDKITNKSTYCVWKSTTN"
     gene            complement(2807..3574)
                     /locus_tag="VHJ57_00020"
     CDS             complement(2807..3574)
                     /locus_tag="VHJ57_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2486953.1"
                     /translation="MIELFIIMVIIGFGSGVIGSLIGVGGGIFMTPVLTYMGFSPAVI
                     ASSSLIAILATSISSTLTYIRKKYINYSLGLKLALPAIPGSIIGGFFSNFISLEHFKI
                     YFAILLTSVGLYIFFKNKIIKKTLDRTPKLLFYLILISGTFCAGVISSLFGIGGGIIF
                     VPILVIMYKMKMINVSPTAQFTLLITTITGLLTHIILEHPDYSYGIALAFGAFFGAQV
                     GSRSIHLISENILSKILSFSLISIAINFIIDYIKSLK"
     gene            3794..>4749
                     /locus_tag="VHJ57_00025"
     CDS             3794..>4749
                     /locus_tag="VHJ57_00025"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX2486954.1"
                     /translation="MRIILIGYGSVGQSFTKLLLSKLVDLYNLYGMKPRIVACVDNKG
                     SAIDKSGLDIQRLLEIKKNKGTVGEYYKNKISRLEPLEIIENIDAEIVMELTPTNLKD
                     GEPGLSHIISAMKYGKNVITVNKGPLSVAFPSLIELANYNGIMFKFSGTVGGGTPILE
                     FAKRCLKGDRIVSFKGILNGTTNFILSKMEEGLTFKNALKDAQIKGYAETVPSLDIDG
                     LDAAAKLVIMANWLMGMKVALEDVHRSGITKVTTQDVSNALKKGNAIKLIASCENKKL
                     VVKPMEVSKVDPICVNGTLNSVTFSLEYAGNQTIIGRGAGGI"
BASE COUNT         1669 a          754 c          729 g         1597 t
ORIGIN      
        1 ttgtttgata tataattcac ctaattattt taatggaatg taagcatatt atcaactttt
       61 tcttcctagt aatattataa tttaaatggt aattatatcc aaaaaactag cgatcacata
      121 atcttgtagt attaagcttc acttattgaa atagaaatta tgtaatctca atttttttga
      181 tttttttgac tcattgatta ttacataatt tttacacaat tcacaatgta ttatttcgat
      241 agtaaacatt agtaagaaaa gagaagaatc taaaattcaa aaaacgtcac gaacatagta
      301 ggtgatctct ttcattggct taatttagta aatcataaat agatgtgcaa aattagatat
      361 agagtcaaca ataaggaact atcataaaga gttaattcga aaaaatgatt aatatccaag
      421 aactgttttc ttaaatcatt ataggttatt gatcactaat gatgaatcta gtagtgaaaa
      481 agaaaaatac aaataactta attattctat ttgaatttat gccaataact gatgctacta
      541 aaaaacaaat tgcacaagcg cgaagattat tctataaaat atgttttaaa tgtggaggta
      601 gaaatcccat ttcttcagaa agatgcagaa aatgtcacag tcagaaaatg aggttaaaga
      661 atagaaacat tggagccaaa aaataatttt tatggttatt ttagactatt tttaatgcaa
      721 tatttttaat ctaccaataa ttcgctttga tcttttcaat agctttttca tgttcaggtc
      781 ctttacttct tgcaaatttt tccatagatg ttaacggtat tccacctagt gtttttgcca
      841 aaccagaaaa gaacgtattt ttaatagtat tatgtctacc tacttttgac ataaattttt
      901 gtataccatg tttaaaattg tgttgccatg ttttatacat tactcctaat tttccaggat
      961 ccattgtatt tccaatgtca aaaaaacttg atttttctag taatccaata ggaacaaaag
     1021 taaggggtgt gacagtaaac ttagaatctg gttgttctgt ttctagttcg tgtataagcc
     1081 taattgtctc ccagctatcg tcgatacttt cgttattatc taagcccatt ataagagtaa
     1141 atgcaggaac ccaataatgt tcatttaagg ttttaacccc ttccttaacc acccaatgcc
     1201 attcagcggg tttaaaagga gctaattttc tgtcagcata ttttcctatt aacctaatac
     1261 taccggtttc taatccacat tgaatgccta tgaaattatc aggaccagat ttaattattt
     1321 ttgaaatatt cggtacaagt ctctcatccg caatcgcacc cgctaacgtc ccatgcgtag
     1381 gattagtatg ttctatgcct gtatccatga ttcctctaaa aagttcctca attgcctctc
     1441 tatttggctg catatttttt gtagttctag gattaagacc ataaacaaaa atatcgtcac
     1501 tttgaatcca ggcgttttta agacccccgt atttcatgtt tatttctatt tctttttgta
     1561 ctttttcgac tggataatat cttaatggac gtaaagtaac atcacagaac ttgcaaccac
     1621 gtccacatcc gcgcataacc tcaatcattc catgcatact gggattaacg atattgggaa
     1681 tttcatctat agagggttca tcagaatagt gaaagaatct accatgatat tttctatcgt
     1741 ttttagcaaa ttctttaatt tccaccttga attcactttt tttgaatgga ttagaggttt
     1801 ctaaatctcc attaattact gcattaaaaa atcgtgctcc tactccatct aattcaggtc
     1861 ctattcctcc aagatctcct tcaacaatac catagattcc atactcttct attttagcag
     1921 gatcgtagtt atattgccag gtaccagaag cacctactat tacctttgct ttactgccat
     1981 tcttagcttt agcagtgttt atacgcaaat gtaattctct gttatataat tcatcataag
     2041 acatagtttt ccttccaaat gtaaaagtca tagttactgg acccattccc aagggatcca
     2101 tttcatatgt acctactact tctgtttcgg gaccgatgaa tttctctata taatcaggat
     2161 gggcaactac aacgtcgttt ggattataac catctcttaa tagagacgct tctaattttc
     2221 tcaatccata gggagcaaag tttgctatac cattttcatt tccaatagga ttacaaatga
     2281 aatcaaatag gaccctagga gttacttgat ttttcaaaat ataataccag aatgaatctt
     2341 tagttctatt gaaatccaaa gcaggagcac aaccaaaaaa agaagctaga gatatgcccc
     2401 gataaggaga cattaaagta cgatcagcag ataatacaac ttttggagat ttcattttat
     2461 ttaatcctaa tcattgaatt tgacataggg atgaatttta acttttccca actttcacct
     2521 agatttattt aaggatttgt tatctgaagc agtatataca attactctta atacaaaatg
     2581 atatattgat atgataaaag gtttatttgt atggaagaaa aaatttattg taatatttgt
     2641 gatgtatcta tttacgttaa tgaaatcgag aatcatatta ctaattctat tcataaaaag
     2701 tcaaaaaacg agtatataca acaactacta tcttttgttg aacgagatga caaaataact
     2761 aacaaatcaa cgtactgcgt atggaagagt accactaatt aaaatgtcat ttaaggcttt
     2821 ttatataatc tattataaaa ttaattgcga tagatattaa agaaaaagat aagattttac
     2881 tcaatatatt ttcgctaatg agatgaatag atcttgaacc tacttgagca ccgaaaaaag
     2941 caccaaaagc aagagcaatg ccataactat agtcagggtg ttctagaata atatgcgtca
     3001 acagacctgt tattgtagtt atcaaaagtg taaactgagc tgtcggactc acatttatca
     3061 ttttcatttt atacattata actaaaatcg gaacgaaaat aattcctcct cctatgccaa
     3121 aaagacttga tataactcct gcacaaaacg ttccagaaat taaaattaaa taaaaaagga
     3181 gctttggagt tctatctaga gttttcttga taattttatt tttaaaaaat atatagagcc
     3241 ctacagatgt taacaatata gcgaaataaa tcttaaaatg ctctaacgat ataaaattag
     3301 aaaaaaatcc tccaattatt gatccaggta tcgccggtaa agctagtttt aacccaagcg
     3361 aataattgat atattttttt ctaatatatg ttaatgtaga tgatatacta gttgccaata
     3421 ttgcaattag acttgaactc gcaatgacag caggtgaaaa tcccatatat gtcaataccg
     3481 gcgtcatgaa tatacctccc cctactccta ttagggagcc tatgacaccc gacccaaatc
     3541 caattatgac cataataata aatagttcaa tcaaattact cttcctatac cttgattttc
     3601 ataatgtaga caagttaaaa tttattctct ttagttctga ctataggatg gagtgatgtt
     3661 caacaatgaa caagtaccat ccaatcagaa ttataacttt taagttgttg gtcacaaccc
     3721 aatgagaaat attttaaaac attttgatta taaaatataa gtatatcatt atttatctta
     3781 ttgttattta gaattgagaa taattttaat aggttatgga agtgtaggtc agagttttac
     3841 taaattattg ctttcaaaat tagtagattt atataatctt tatggaatga aacctagaat
     3901 tgtagcgtgt gttgacaata aaggttctgc aattgataaa tctggcttag atatacaaag
     3961 gcttttagaa ataaaaaaaa ataaaggaac agtgggtgaa tattataaaa ataaaatatc
     4021 acggttagag cctttagaaa taattgaaaa tattgatgca gaaattgtga tggaactgac
     4081 tcctactaat cttaaagatg gcgaacctgg gttatcccac ataatttcag ctatgaagta
     4141 tggtaaaaac gttattaccg ttaataaagg accattatca gtagcttttc catctcttat
     4201 agagttagct aactataacg gaataatgtt taagtttagt ggaacagtgg gtggaggaac
     4261 tcccattctt gaatttgcta agagatgctt aaaaggagat agaattgttt cgtttaaagg
     4321 tatacttaat ggaacaacca attttatttt gagtaaaatg gaagagggat tgaccttcaa
     4381 aaatgctcta aaggatgctc aaataaaagg ttatgccgaa acggttccgt ctttagacat
     4441 agacgggttg gatgctgctg cgaaattggt aataatggct aactggctaa tgggaatgaa
     4501 agtagcactt gaggatgttc atagaagtgg cataacaaaa gtgacaactc aagacgtttc
     4561 taatgcatta aaaaaaggaa atgcgattaa acttattgct agttgtgaaa acaaaaaact
     4621 tgtagttaag cctatggaag tatcaaaggt agatcctatc tgtgtaaatg gaactttaaa
     4681 ttcagtcact ttttcattgg agtatgctgg gaatcagaca ataataggac gaggagcagg
     4741 aggtataga
//
LOCUS       DATAFW010000002         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_704937, whole genome shotgun sequence.
ACCESSION   DATAFW010000002 DATAFW010000000
VERSION     DATAFW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704937"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..680)
                     /locus_tag="VHJ57_00030"
     CDS             complement(330..680)
                     /locus_tag="VHJ57_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEX2486955.1"
                     /translation="MSQDIQQTRRQIFDELSKIVDPEIGVSIMELELIDKVDIEEKDV
                     NVDLHLTSPFCPAVFGFKIAQDVRDNIYKIEGVENVKINVSNHFMAEAINKQVNESKP
                     PSKEEENHENKQQK"
     gene            805..2346
                     /gene="argH"
                     /locus_tag="VHJ57_00035"
     CDS             805..2346
                     /gene="argH"
                     /locus_tag="VHJ57_00035"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX2486956.1"
                     /translation="MIDILYRSRQKENLDKNVLNFLSSLNEDANILSYDILGTQAHCL
                     MLHKIGMLTANELSHILSSLRKIKFDSKNRGKIQNILSNELYLSEDIHELIESIVIED
                     IGLDIGGKMHTGRSRNDQVILDIRMKLRDDIIDICELIINVIKSLVRKSKEHTNTIIS
                     LYTHLQQAQIGTVSHYLLSYSFTLLRDLERVSSSFEKNNLSPLGSCAIGGTGISIDRN
                     YTASLLGFKGLLENSIDATSSRDSLIEYLSFLSILMSTLSRIAEDFILWSTSEFNYIE
                     LSDKYSSTSSVMPQKKNPDPLEIIRSRTSIVMGMLMSSLSLIKNLPSGYHRDLQDLKP
                     LLSKSTTIVKESLSIMKGVIDTFIVNEKNTCNSANKSYGVSLDIAEQIVIKYNISFRK
                     VHKLMGALVQHAIKNENISLSEIAKEDVNKILKFTKIKIDVDELIYIIKDTTPEKSLL
                     LRNSYGSPNPMQQKEMVISIEKSLLVYGKVISQRKKLLDAAFDNLEKEVTLNTIFDNK
                     KKIKP"
     gene            complement(2421..2520)
                     /locus_tag="VHJ57_00040"
     tRNA            complement(join(2421..2483,2511..2520))
                     /locus_tag="VHJ57_00040"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2457..2459),aa:Gln,seq:ttg)
BASE COUNT          988 a          378 c          382 g          943 t
ORIGIN      
        1 tttctaagag tgaaaaaaaa attgcacaac ttcaagatag atctactaat tcttacagag
       61 aagaagacgg agaaaaacaa atagaaaaag aagatttaaa ggaataattc tataatccaa
      121 attctaaaaa aaatagaagt ataattatgt tatagttttt tactcctttc aaacgacaac
      181 aatgagtatt attattacta ttaatagtct tagtttttta atctgttgat tgcatatgca
      241 gatcttcctt agagtagata aaaatataga ataaaaatag ttataataaa aatattaagg
      301 tagtacctga ctattaatat acacgaaatt tacttttgtt gtttattttc gtgattttct
      361 tcctccttag aaggaggttt gctttcattt acctgcttat ttatagcttc agccataaaa
      421 tgattgctca cattaatttt tacgttttcg actccttcta ttttataaat attatctctt
      481 acatcttgag caattttaaa accaaaaact gcaggacaaa atggacttgt taaatgtaaa
      541 tcaacattta catctttttc ttcaatatct accttatcaa tcaattctaa ttccataatg
      601 gataccccta tttcaggatc tacaattttt gataattcat caaaaatttg cctacgggtt
      661 tgttgtatat cttgagacat tatataaagt agtcaaaaac catatttaac tatttattga
      721 atttccttct tataatttgt cataatccat tgtaaggtta taccagaaga ataaatgaat
      781 atattatatc tttttcaatt tagtatgatt gatattttgt atcgctcgag acaaaaagaa
      841 aatttggata aaaatgtatt aaattttctt tcttctctta atgaagatgc taatatactt
      901 agctatgata ttttaggcac tcaggctcat tgtctaatgt tacacaaaat tggaatgtta
      961 acggctaatg aattatctca cattctttca tctttaagga aaataaaatt tgattcaaaa
     1021 aataggggga agatacaaaa tatactttca aatgaactat atctttcgga agatatacat
     1081 gaactaatcg aatctatagt tattgaagat attggattag atataggagg aaaaatgcac
     1141 acaggacgat caagaaatga tcaagtcatt ttagatataa gaatgaaatt aagagatgat
     1201 ataatagata tttgtgaatt aattattaat gttattaaat ctcttgttag aaaatccaaa
     1261 gaacacacaa atacaataat atcactatat acccatttac aacaagcgca aataggaacg
     1321 gtatctcatt atcttttatc atattctttt actcttttaa gagatcttga gagagtaagt
     1381 tcctcatttg agaaaaataa tttatcacca cttggatcct gtgccattgg tggaacagga
     1441 atatccatag atagaaacta tacggcttct cttttaggat ttaaaggctt attggaaaat
     1501 tctatcgatg cgactagctc aagagattca cttatagaat atttgtcttt tctttcaatc
     1561 ttgatgagta cgcttagtag aattgctgaa gattttattt tatggtctac ttctgaattt
     1621 aattatattg aactatcaga caaatatagc tctacatcca gtgttatgcc acaaaagaaa
     1681 aatcctgatc cccttgagat aattagatct cgtacttcaa ttgttatggg aatgctaatg
     1741 tcttcacttt ctctaataaa gaatcttcca tctggatatc atagagactt acaggacctg
     1801 aaaccacttt tgtccaaatc aacaacaata gttaaagagt ctttatctat aatgaaaggt
     1861 gttattgata cttttatcgt gaacgaaaaa aatacttgta attcagcaaa caaaagctat
     1921 ggcgtatctc ttgatatagc cgagcagatt gtaattaaat acaatatttc atttagaaaa
     1981 gtacacaaat taatgggtgc attagtacaa catgctataa aaaatgagaa tatttcctta
     2041 agcgagattg ctaaggagga tgtaaataaa atattaaagt tcacaaaaat taaaatagat
     2101 gttgatgaat taatttatat cattaaggac acaactcctg aaaaatcact attactcaga
     2161 aactcgtatg gatcaccaaa cccaatgcaa caaaaagaga tggtaatttc aatcgaaaaa
     2221 tcactattag tttatggtaa agtaatatca caaagaaaaa aattgttaga tgcagcattc
     2281 gataacttag agaaagaggt tactttaaat actatttttg ataataaaaa aaaaattaaa
     2341 ccttaataat ttttatcttc tcgttagttg ttatttgttt ctataaatag gaaattagaa
     2401 atttattaat aaatatttta tagcccggcc cagattcgaa ctggggtcaa aggctccaaa
     2461 ggcctttatg cttggccgct acaatcggcg gaaaataaat aatatttcct ccaccgggct
     2521 tccgggcata atgtcgtgta aatcactagg attataatgt tatggattaa agagaaattt
     2581 tttgaatatt tttataggat cttccatttc tgctataatt tttaaagata tttcctgtat
     2641 ggtattagga tcagatttta tattgggaat ttttttattt tttgttgttc c
//
LOCUS       DATAFW010000003         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5990554, whole genome shotgun sequence.
ACCESSION   DATAFW010000003 DATAFW010000000
VERSION     DATAFW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5990554"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..773
                     /locus_tag="VHJ57_00045"
     CDS             414..773
                     /locus_tag="VHJ57_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486957.1"
                     /translation="MKREFIASRIEATQDGSPYVYVTFSDAHDYKPGSDKRMNPFGPN
                     VMAFTSPEDLMKNLPKAMGDISKMISGGMGGVAGGMGGDNPTFKITMKDYEDMNIKVG
                     DKVTIEIKKSDSGSIYT"
     gene            952..1401
                     /locus_tag="VHJ57_00050"
     CDS             952..1401
                     /locus_tag="VHJ57_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486958.1"
                     /translation="MVNIDTTASLESFRKFIIVSTCKSFIPESYFRDYEVFPEREDGP
                     GAIYIEAVDKVTLKRIREMTFVNAKDILGIIYSSKSGNTQLKWRQIRKKSGKVNGTAS
                     PNALVNLVESGVLTQEWVDDYLASAKNLNKSTNQSNETSKSNSSPAV"
     gene            1437..>2492
                     /locus_tag="VHJ57_00055"
     CDS             1437..>2492
                     /locus_tag="VHJ57_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein Pelota"
                     /protein_id="HEX2486959.1"
                     /translation="MKSKEVSNSSGKAMLIIPEDSDDLFTLRRIIKTDNIVVSDTSRV
                     IKYEKEYSRPDRERVKIRVSVKVEKIALDESIDRLRIAGTIIESNNSLVPKGTYHSII
                     VQIGNNIVIDKGKKWEELDLKLLKSNFSGTFVIVAIDTKEAGIAKITGTHLEIIPNIY
                     SGQSGKYYTQGAKNNPNIEVFFEEVYLALHNIVNEDGNYKIIIFGPGETKRRFSNFII
                     GKKSSYKDILTVIDGIDIAGEDGVMVFLRSPLLKEVMSSSKISFVSAILDNIMLQVSR
                     GEEKYVMGFREVGEAINFKSVESLVFSDSIFKDLKEEQIINMLNTAETYGANVYAVDS
                     STDIGLRVTALGGIVALL"
BASE COUNT          948 a          305 c          444 g          795 t
ORIGIN      
        1 gtgaaacatg gtggaaagat aaagaaacag gcaagtattt acagcaaata atgtcttcag
       61 gtgcagagat tgatctttca gtattttcaa agcttgatag tgatatatat ttaaaagaaa
      121 tcacatctat ataataggta aatatctaaa ttatagttgg aatctatttc atgaaattct
      181 caaattacta tttttagatg ctgtgaggtt ttctatatta gcgtctataa gaactgctat
      241 aatacttcta atatattagg tatgggagac tgctttatgc tattataaca ggataaattc
      301 tatggatctg ataatttgta gaaaatgatg attatctatt tcataatcca attaatagag
      361 atcactaaaa tatccaaaat tataaattga aataactatc attttaattt agaatgaaac
      421 gagaatttat tgcctctaga attgaggcca cacaagatgg cagtccttat gtttatgtaa
      481 cattctccga tgctcacgac tataaaccgg gaagtgataa aagaatgaat ccttttggtc
      541 ctaatgttat ggcatttact tctccggaag atttaatgaa aaatttacca aaggcaatgg
      601 gtgatatatc aaagatgatt tctggaggta tgggtggagt agcaggagga atgggaggag
      661 ataaccctac ctttaagata acgatgaaag attatgagga tatgaatatt aaagtcggag
      721 ataaagttac tatagagata aagaagtctg attcaggcag tatttacaca taattactca
      781 tcttaaatca ttttattttt cgtatttata atatacttgt agataatatt gtaaactgcg
      841 tacccaccca gtagattata ataacaatac aagaatatac cagacagatt ttctaaaagg
      901 tattttctgt ttcataaact tgataaatag cacgttgttt ttaagaatat attggttaat
      961 atagatacta ctgcatcact tgaaagtttt agaaagttta ttattgtaag tacatgtaaa
     1021 tcttttattc ctgaaagtta ttttcgtgat tatgaggtct ttccggaacg agaagatgga
     1081 ccaggagcca tttatataga ggctgttgat aaggtaactt taaaacgaat tagagaaatg
     1141 acttttgtaa atgcaaaaga tattctagga ataatatatt cttctaaaag tggcaatact
     1201 caattaaaat ggagacaaat taggaaaaaa tcaggcaaag ttaatggaac ggcatcacct
     1261 aatgcattgg ttaatcttgt tgaatcagga gtcttaacgc aagaatgggt ggatgactat
     1321 cttgcatctg ccaaaaattt aaataaatca actaaccaat caaatgaaac ctccaaatca
     1381 aattcaagtc cagctgtttg atttgaaaaa tcaacttacc tgctaattat ataaatatga
     1441 aaagtaaaga ggtatcaaat tcttctggca aggctatgtt aattattcca gaagattctg
     1501 atgatctttt tactttaaga aggatcatca aaacagataa tatagtagtg tctgatacct
     1561 ctcgagtaat aaaatatgaa aaagaatatt ctagaccaga tagagaaaga gtaaaaattc
     1621 gcgtatcagt taaagtagag aaaatagcac tggatgaatc gatagataga ttgagaatag
     1681 ctgggactat aattgaatca aataatagtc ttgtaccaaa gggaacatat cattcaatta
     1741 tagttcaaat tggaaataat attgttattg ataaaggtaa aaaatgggaa gagctagatt
     1801 taaaactttt gaaatcaaat ttctccggta cttttgttat tgtagccatt gatacaaagg
     1861 aagctggaat tgcgaaaata actggaactc atttagaaat cattccaaat atttactcag
     1921 gacaaagcgg aaaatattat actcagggag ccaagaataa tcccaatata gaagtgttct
     1981 ttgaagaagt ataccttgca ttacataata tagtaaatga agatggaaac tataaaataa
     2041 taatatttgg tccaggagaa acaaaaagaa gattttccaa ttttattata ggtaaaaaat
     2101 cttcttataa agatatatta actgttattg atgggattga tatagcagga gaagatggag
     2161 taatggtatt tctcagatct ccattactca aagaagttat gagttctagt aaaatatcat
     2221 ttgtatcagc aatattagac aatatcatgt tacaagttag tagaggtgaa gaaaaatatg
     2281 ttatgggatt cagagaagtg ggtgaggcaa ttaattttaa atctgttgaa tctcttgttt
     2341 tttctgattc tatatttaaa gatttaaagg aagaacaaat aataaacatg ctaaatacag
     2401 cagaaacata tggagctaat gtatatgctg ttgactcttc aacagatatt ggattaagag
     2461 taactgcatt aggtggaata gttgcattgc tt
//
LOCUS       DATAFW010000004         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_176503, whole genome shotgun sequence.
ACCESSION   DATAFW010000004 DATAFW010000000
VERSION     DATAFW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176503"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..543
                     /locus_tag="VHJ57_00060"
     CDS             43..543
                     /locus_tag="VHJ57_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486960.1"
                     /translation="MSIQSDKKIEKAHKLDKIDYKIICQLLLGSDSKSISSILHIALS
                     TIQRRTRAIIFANIINREYTPNCQILGISKVLLHIYLSDGKLRETAEKISEMEGIISV
                     SLHIGNSDILAEFVYVNADSIVDIISSIKKIEGIERIVWSQEIENIIISKDKIMKTYQ
                     KLIEKL"
     gene            1203..1682
                     /locus_tag="VHJ57_00065"
     CDS             1203..1682
                     /locus_tag="VHJ57_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486961.1"
                     /translation="MLLKIGDSKMPRVEHYTGLQTNLDNLYNSIKKEIEEEKNLRIVS
                     EIKGEMNGKPLRSITAVNTSIVVLAGALREITVSIIGDTDEFAIEVASGSWFESLLIP
                     GVTGFIVGGPLGAVGGTTVGIVMAYQFERKIWKKIQEALQRESKTQPTVQNTEHYSK"
     gene            complement(1959..2201)
                     /locus_tag="VHJ57_00070"
     CDS             complement(1959..2201)
                     /locus_tag="VHJ57_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486962.1"
                     /translation="MLIFLIIVFINFNLNPLFTYGTSTVLEICDNKLDDDNDDIVDEP
                     LCVNESTVLEICDNKLDDDNDDIVDEPLCVIQNIVS"
     gene            complement(2430..>3918)
                     /locus_tag="VHJ57_00075"
     CDS             complement(2430..>3918)
                     /locus_tag="VHJ57_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2486963.1"
                     /translation="DTDSVIKSINKVKIDYYLMKPWDPAEVHLYPVLNEILDDWWASC
                     HPPFEGIKVIGLRWSPRSHEIKDFLARNGIPYQWLDIEGDEEARRLVSIINSTWSDIS
                     TTNNNNSNTFSFNSDASSISSSSSSSSSSIHLPLVIFPDGSYISEATNSQIAEKVGLK
                     TRAQMAFYDLVIIGGGPAGLAAAVYGSSEGLHTLLVERQAPGGQAGLSSRIENYLGFP
                     SGLTGGDLARRAVTQAVRFGTEILDPQEVVDIRVEDPFRIAKLADGTEIRCHVMLIAC
                     GVTYRKLDDVKGIDKLVGAGVYYGASMVEALHYKDQDVYIVGGANSAGQAAIHFSKYA
                     KQVTLLVRDDSLNKKMSQYLVHQINETDNIMVWLNTKVTEVRGEKRLEDITVTNTKTG
                     EQQVVKAAGLFIYIGAEPHTKWLPRIIQCDIHGFILTGSDFIQNELQRPQGWSMDRQP
                     FLLETSIPGIFAAGDVRHGSIKRIAAGVGEGSTAIQLIHQYLTKV"
BASE COUNT         1420 a          590 c          604 g         1304 t
ORIGIN      
        1 atattacaat gatattactc aatcatttgt ggacctatta aaatgtcaat acaatcagac
       61 aaaaaaattg aaaaagccca taaattagat aaaattgatt ataaaataat ttgccagtta
      121 ctcttgggtt ctgatagtaa aagtatatct tcaatattac acatagcatt aagtacaatt
      181 caaagaagaa cgagagctat aatatttgca aatataataa atagagaata tactccaaat
      241 tgtcaaattt taggaatttc taaagtatta ttgcatattt atctaagtga tggtaagtta
      301 agagagacag cagaaaagat atcagaaatg gagggaataa tatcagtaag cctccacatt
      361 ggtaattcag atatactagc agaatttgta tacgtaaatg ctgattctat agtcgacatt
      421 atatcatcaa ttaagaaaat tgaaggtatt gagagaatag tgtggtctca agaaattgag
      481 aatataatta tctctaagga caagataatg aaaacatatc aaaaattaat agaaaaatta
      541 taacgatggt aaaaataaat ctatagtata gtgaaatgca taaatctata tatccatatc
      601 cagtgtgtat attttctaac ctatttgtat taaataatat tgttaataac tattagttgt
      661 attaataagg tcatatttta tactattaaa ggaacttatt tttaattagc aatacaaaat
      721 gagtattaaa tgtaattgta tatatatagt ggataactta tttaatcaat taatctaaaa
      781 ttgattgttt tatagttaaa actttaattt tctttaaagt attttatttt atctaaaaag
      841 tttttaattt ttcaaaataa aagataattt gaatttcttc aacttcatca cctatttaaa
      901 ttaccatgat tacaaattat aacttattaa aaatgttgct tctatgggaa tagattcttt
      961 tttaagtcta atttatcata tacaaaagca tttttgaaac atatattttg ctaaacctta
     1021 aaatgaaatc tatttcaata tgaatacgta aatagtttgt agaataataa ttgttattga
     1081 aaatgtacaa gttgaatttg aggaatatac aagctgatga ttttttccga agattattta
     1141 gtcaagaaag tagtagacta acaatatcag caggaagcaa tggaggagaa cagtagtgat
     1201 ctttgctttt aaaaattgga gatagtaaaa tgccaagagt agaacattat acaggccttc
     1261 aaaccaatct tgataatctc tataatagta taaaaaaaga aatagaagaa gaaaaaaatc
     1321 taagaattgt ttcagaaatt aaaggagaga tgaatggtaa gccattaaga agtataacag
     1381 cagtaaatac ttcaattgta gttttagcag gtgctttgcg agaaattacc gtatctataa
     1441 taggggatac agatgagttt gccattgaag ttgcttccgg gtcatggttt gaaagtttac
     1501 taattccagg tgttactgga tttattgttg gaggaccatt gggagccgta ggaggtacaa
     1561 cagtaggcat agtaatggca taccaatttg aaagaaaaat atggaaaaag atacaagagg
     1621 cacttcaaag agaaagtaaa actcaaccta cagtacaaaa tactgagcat tatagcaaat
     1681 aatagttagt taactcaatg ggaatagatt gtatgtgtga tgatataata ttaatataaa
     1741 aacgaaaatg atgaaaatgg attaaacaag aagaaaaagt tggttataac ctcaaacgat
     1801 taagcaatac aaaaatgttt cagattttct tattcttaat ttttcaaaag ttaattcacc
     1861 taatttagta aactttgaaa attaaaaata aataataaat tttgtaaaat tctactatca
     1921 aactataaca aattaacagc taagcctatt tcggatgatt aagagacaat attttgaata
     1981 acacaaagtg gttcatctac aatatcatca ttgtcatcat ccaacttgtt gtcacatatt
     2041 tctaatactg tgctttcatt aacacaaagt ggttcatcta caatatcatc attgtcatca
     2101 tccaatttgt tgtcacatat ttctaatact gtgctagttc cataggtaaa aagaggatta
     2161 aggttaaaat ttataaatac tataattaaa aagattaaca atttggagaa tttcacaaat
     2221 tatacttatt taggtttttt ataaagattt ttgatgttga tggtattagt tatttatgtg
     2281 atttcatata atattatata catttaccat ttatttatta tctaattgta acacaaagtt
     2341 aaatatattt atatttcata tccgctgagt agtagactaa aagcaaacct tgttatatac
     2401 ttcatttcga tacacatatt agtattcttt tatactttgg ttaaatattg atgtattagt
     2461 tgaattgctg tagagccttc tccaacccct gcggcgatcc tcttgattga accatgacgt
     2521 acatctccag cagcaaatat accaggaata ctagtttcaa gtaaaaaagg ttgacgatcc
     2581 attgaccaac cttgaggtcg ttgaagttca ttttgaataa aatccgatcc agtcagaata
     2641 aaaccatgta tatcacactg tattattcta gggagccatt ttgtatgtgg ctcagcacct
     2701 atatatataa aaagtcctgc agctttaaca acttgctgtt ctcctgtttt tgtattggtt
     2761 acagtgatat cctcaagcct cttttcacct cttacctcag tcacttttgt atttaaccat
     2821 accatgatgt tatctgtttc atttatttga tgtacaagat attgggacat tttcttgttt
     2881 agagaatcat cacggactag gagagttact tgtttagcat attttgagaa atgaattgcc
     2941 gcttgtcctg cagaatttgc tccaccaaca atataaacgt cttggtcttt atagtgtaag
     3001 gcttctacca ttgaggcccc ataataaaca cccgctccaa ccaatttatc aattcccttt
     3061 acatcatcaa gcttacgata tgtcactcca caagcaataa gcattacatg acatcttatc
     3121 tctgttccat ctgccaattt ggctatacga aatggatcct caactcgtat atctacaact
     3181 tcctgtggat ctaaaatttc agtaccaaat cgaacagcct gagtaacagc acgtctagct
     3241 aaatcaccac cagtcaaacc ggaaggaaat ccaagataat tttctattct agagctcaat
     3301 cctgcctgtc ctccgggagc ttgtctctca actaacaatg tatgtagtcc ttctgatgat
     3361 ccatatacag ctgcagccaa ccctgctggg cctcctccta taattaccaa atcataaaaa
     3421 gccatctgtg cacgagtctt gagaccaact ttttctgcaa tttgggagtt ggttgcttcc
     3481 gatatgtatg agccatcagg aaaaataaca agcggtaggt gtatagaaga agaagatgat
     3541 gaggatgatg atgagattga tgaggcatct gaattaaacg aaaatgtatt tgaattatta
     3601 ttattggtag tagaaatatc tgaccaagtt gaattaatga tggaaactaa acgacgtgct
     3661 tcttcatctc cttcaatatc taaccattga taaggtatgc catttctagc cagaaaatct
     3721 tttatctcat gtgaccttgg tgaccatcta agacctataa cctttatacc ttcaaaagga
     3781 gggtggcatg aagcccacca atcatctagt atttcgttga gaactggata aagatgaact
     3841 tccgctggat cccacggttt catcaagtaa taatcaattt ttactttgtt aatagacttt
     3901 attacagaat ctgtatct
//
LOCUS       DATAFW010000005         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_176807, whole genome shotgun sequence.
ACCESSION   DATAFW010000005 DATAFW010000000
VERSION     DATAFW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176807"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VHJ57_00080"
     CDS             complement(<1..1302)
                     /locus_tag="VHJ57_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEX2486964.1"
                     /translation="MLDNLRTGLRSALKKIVGASDINEELIDSLSKDIQRSLLQSDVN
                     VRQVLSITKNLKYRALNEPPPKGLTKKDHIITILYNEISSLLGYTGNQIIHIDKTPSM
                     PEDSFNFQPGKKNIVLMLGIQGSGKTTCTVKIARWLSRHGYKVAIIGADTWRPGALTQ
                     LKMNSIKIKVEVYGEEENKNPISIVKNGLEYYKSQDYDVIVIDTAGRHKEESGLLEEM
                     NEIHKVTNPDLVLLVIDGTIGQQAYNQAKAFHDSAPIGGIIITKLDGTAKGGGALAAS
                     AVTGAKIFFIGTGERIDDLELFSPTRFVGRLLGMGDIKALLDMAKRLEIEADETQAKR
                     LLGGKMTIEDFYSQMENVNKMGFRNVIENLPGLSGMVKDDQLEVLQGKIEKWRFIIQS
                     MTKDEKKDPNIINDARRKRISRGSGITEHEVKDMIKQYNNSK"
     gene            complement(1303..1989)
                     /locus_tag="VHJ57_00085"
     CDS             complement(1303..1989)
                     /locus_tag="VHJ57_00085"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017178 - diphthine-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEX2486965.1"
                     /translation="MRVACLFSGGKDSTYSLYLAKQWGLSVDCLLTLIPHSEESSLFH
                     CPNIWTTQLQSQALDIPIITKKINSISIDEEYNKLDELISQANNNYKIEGIVHGGIRS
                     NFQKNKFDKICKKYNLKLISPLWHIDDYYYMNILLKNCFVIQITSVSSMGLDANFLGI
                     ILNDSTLEDLYRRSRKYGFNLSFEGGEAETLVLDCPIFKKKLEILTSNIHWDGQRGIV
                     EISDISLLNK"
     gene            complement(2054..2335)
                     /locus_tag="VHJ57_00090"
     CDS             complement(2054..2335)
                     /locus_tag="VHJ57_00090"
                     /inference="COORDINATES: protein motif:HMM:NF026297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2486966.1"
                     /translation="MKNRAEIEVIAAILRSAKDNWEYQTTIMNNACISHSQVIRYISV
                     AMNRGLIEYSKISGLYKTTNMGLEFLEKHAKLLTLFPEIAEPSSQFPYN"
     gene            2663..>3330
                     /locus_tag="VHJ57_00095"
     CDS             2663..>3330
                     /locus_tag="VHJ57_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2486967.1"
                     /translation="MICARIVKSNNPLVIEDIPVPKPKNFEVLVRIKAAGICHSDLHL
                     YEGGYMGAGGKFMTVEDRGVKFPLTPGHEVSGVIEDIGESVKHYQKGDKVLIYPWIGD
                     EFCHSCKDGNEHLCESPKTLGIYQDGGYADLILVPNYKYLIKLEDIDFNSSAALACSG
                     LTAYTSIKKSLAMPGETLVLIGAGGLGLMAVQIAKAITNSNIIIMDVDDEKLKEAKKL
                     GADFT"
BASE COUNT         1053 a          557 c          494 g         1226 t
ORIGIN      
        1 tttagaatta ttatattgtt ttatcatatc ttttacctca tgttctgtaa ttccagaacc
       61 acgagaaatt ctttttcttc tagcatcatt gataatatta ggatcttttt tctcatcttt
      121 tgtcattgac tgtattataa acctccattt ttcaatttta ccttgtaaga cttccaattg
      181 atcatctttt accatccctg ataagccggg taaattttct ataacgtttc taaatcccat
      241 cttattaaca ttttccattt gagaatagaa atcctctatt gtcatttttc ctcctaaaag
      301 cctcttagcc tgagtttcat ctgcttcaat ctcgagtctc ttagccatat ctaggagggc
      361 ctttatgtca cccataccaa gtaatcgtcc cacaaatcta gttggagaaa ataattctaa
      421 atcgtctata cgctcacctg tacctataaa aaagattttt gctcctgtaa ctgcagatgc
      481 agccaaggca cctccaccct ttgctgttcc atctagtttt gttataatta ttcctcctat
      541 aggtgcggaa tcatgaaaag cttttgcctg attataagct tgttgtccaa ttgttccatc
      601 tataactaaa agaactaagt caggattggt caccttgtgt atttcattca tctcttcaag
      661 tagtccagat tcttccttat gtcttcctgc cgtatcaata acaataacat cataatcttg
      721 agatttataa tattctaatc cattttttac tatggaaatt gggtttttgt tttcctcttc
      781 gccatacact tcaactttaa tttttatact attcattttt aactgtgtaa gagcgccagg
      841 tctccaagta tcagcaccga ttattgctac cttatagcca tgtcgtgaaa gccatctagc
      901 aatttttacc gtacatgtag ttttgccact tccttgaatg cccaacatga gtactatatt
      961 tttttttcct ggttgaaaat taaaagaatc ttctggcatg gatggtgttt tgtctatatg
     1021 gataatttgg ttaccggtat agccaagaag agaagaaatt tcattatata agattgtgat
     1081 tatatgatct tttttggtta atccttttgg tggtggttca ttcaatgctc gatacttcag
     1141 atttttggtt atcgaaagaa cttgtcttac attaacatct gattgtaata atgatctttg
     1201 aatatcctta gaaagagaat ctataagttc ttcgttaata tcggatgcac caactatttt
     1261 ctttaatgcg cttcttaatc ctgtcctgag attatctaac atttacttat ttaatagaga
     1321 tatatccgat atttctacta tacctctttg tccatcccag tgtatatttg aggttaaaat
     1381 ttctaatttt tttttgaaaa taggacaatc tagaactagt gtttctgctt ccccaccttc
     1441 aaaagagagg ttaaatccat atttcctact ccgtctatag aggtcttcca atgttgaatc
     1501 atttagtata attccaagaa aattagcatc aagacccatg gaagatacac ttgtaatttg
     1561 tattacaaaa caatttttta gcagtatatt catataataa taatcatcaa tatgccataa
     1621 aggtgaaatg agctttaagt tatatttttt acatatctta tcaaatttat ttttctgaaa
     1681 attggaacgt attccaccat gaacaattcc ctcaatttta taattattat tcgcctgact
     1741 aattaattca tctaatttat tatattcctc atctatagaa attgaattta ttttttttgt
     1801 gataatagga atatctaatg cttgagactg taactgggta gtccaaatat tgggacaatg
     1861 aaataaagaa ctttcctctg aatgaggtat taaagttaag agacaatcaa ccgataaacc
     1921 ccactgttta gccaaatata aagaatatgt actatccttt cctcctgaaa ataaacatgc
     1981 tactctcaaa tacaaaattt aaaatgaatt actagtattt attaaaatct aaaataaagt
     2041 gaaggttcat ttcttaattg taaggaaatt gagaagaagg ttctgcaatt tcaggaaata
     2101 aggttaataa tttagcatgt ttttctagga attctaatcc catgttagtt gttttatata
     2161 atccactaat tttagaatat tcaattaggc ctctattcat ggccactgat atgtaacgaa
     2221 taacttgaga gtgagatata catgcattat tcattatagt tgtttgatat tcccaattat
     2281 cttttgcact acgaagaatt gcggcaatta cttcaatctc tgcacgattt ttcatttagg
     2341 tactctttcc tccatttgaa tctgtattac aacacttatt tatcaagaat aaaaaaacaa
     2401 ttgacaatta atcaagacta atttgtttga aaatttataa caatttaatt tcttttcttt
     2461 acaactggta acacctcagt aactcataat cttcttcata tacaagtgta tcagacggct
     2521 cttcctatac ttcattggtg tttgtatgag ttattgtttc tatactatat attaataaaa
     2581 acattgtgta ataatccctc ctatctataa aaaaagtatt ttacatattt catttttatt
     2641 atccatagct tttacataat tgatgatatg tgctagaatt gtaaaatcaa acaatcctct
     2701 tgtcattgaa gatatacctg ttccaaagcc aaagaatttc gaagttttag ttaggataaa
     2761 agctgctgga atttgtcata gtgatttgca tctatacgaa ggaggttata tgggggcagg
     2821 agggaaattt atgacagtag aagatcgtgg ggtaaaattt cctttaaccc ccggtcatga
     2881 ggtttcagga gtaattgaag atataggaga atcagtaaaa cattatcaaa aaggggataa
     2941 ggtgttaata tatccttgga ttggcgatga attttgtcat tcttgcaagg acgggaacga
     3001 acatttatgt gaatcgccta aaactcttgg aatatatcaa gatggaggtt atgcagattt
     3061 aattttagtt ccaaattata agtatcttat taaattagag gatatagatt ttaactcatc
     3121 tgcggctctt gcatgttcag gattgacagc atatacttca ataaaaaaat ctttagcaat
     3181 gcctggagaa actcttgtat taattggtgc tggtgggtta ggacttatgg cagttcagat
     3241 tgccaaagca ataacgaatt ccaatatcat aattatggat gtcgatgatg aaaaattaaa
     3301 agaggcaaaa aaattaggag cagattttac
//
LOCUS       DATAFW010000006         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_529273, whole genome shotgun sequence.
ACCESSION   DATAFW010000006 DATAFW010000000
VERSION     DATAFW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529273"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VHJ57_00100"
     CDS             <1..625
                     /locus_tag="VHJ57_00100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2486968.1"
                     /translation="KLETLKNEVQKYGGEILVKKLDVTKKEDCDSFINLVTKTWNHID
                     VLINNAGIMPLSFFKNLKVSEWEQMIDVNLKGVLYCTAAVIPYMITKKSGHIVNISSV
                     AGRLVFPAGSIYCATKHAVTAFSEGLRLELSQRYNIKVTCIEPGVVSTELPNTITDKS
                     LESFVESVKEMESIKAEDIANAIIYSIDSPPYLNINEILLRPLSQEK"
     gene            1121..1256
                     /locus_tag="VHJ57_00105"
     tRNA            join(1121..1130,1162..1173,1192..1207,1222..1256)
                     /locus_tag="VHJ57_00105"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1204..1206,aa:Gln,seq:ctg)
     gene            complement(1254..>1758)
                     /locus_tag="VHJ57_00110"
     CDS             complement(1254..>1758)
                     /locus_tag="VHJ57_00110"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX2486969.1"
                     /translation="AMYHFMSGYTSKLAGTERGITLPKETFSQCFGAPFMPLHATKYA
                     EMLGNKITLHNTKVYLINTGWYGGPYGIGKRIDLKYTRSMVTAVINGSLEKSSFKTHH
                     IFNLDIPLECPGVPNQLLEPVMTWDNQEEYIQAATKLANLFIQNFEKFGDVSKRIIEA
                     GPKKINL"
BASE COUNT          622 a          242 c          281 g          613 t
ORIGIN      
        1 gaaactagaa actttaaaaa atgaagttca aaaatatggt ggtgaaatat tagttaaaaa
       61 gcttgatgtt acaaaaaaag aggattgtga ttcatttatt aacttagtaa ctaaaacatg
      121 gaatcatatt gatgttctca tcaataatgc tggtattatg ccattaagtt tttttaaaaa
      181 tcttaaagtt agtgaatggg aacaaatgat tgatgtaaat ctaaaaggag tattatattg
      241 tactgcagct gttattccat atatgattac aaaaaaatcg ggtcacattg ttaatatttc
      301 ttcagtagca ggaagacttg tttttccagc tggaagtata tactgtgcta ctaaacatgc
      361 tgttacagca tttagcgagg gattaagact agaattaagt caacgttaca atatcaaagt
      421 aacctgcatt gagcctggag ttgtctctac agaattacca aatacaatta ccgataaatc
      481 tttagaatcg ttcgtagaat ctgtaaaaga aatggaatcc ataaaggcag aagacattgc
      541 taatgcaatt atatattcta ttgattctcc accatatctc aatattaatg aaatattatt
      601 aaggcctttg tctcaggaaa aataactttt tttattattt atattattca taaaaatgat
      661 aaaaacagag gttgtataaa acgaatttta ggtcaaagat ctaagaaatt aagttttatc
      721 actagtccta ttagtgacca gaatttttca ttgtagtctg gaatgcacaa attctttaaa
      781 ttctttagtt ttttagatta ttctatggaa gaaaattgca atattgtaat aaataacttt
      841 ttaattaata taagatgtgg gtatacaata acaataaaat atctgttaat gttttttcat
      901 cttttaaggt aaaattttta taaatcaaat gctccctagt tacaatgatt tcatgaaatt
      961 atacttgaat aaatatttta tatttaatta aaaatttctt taaatgttgt aataaagtta
     1021 gcaaaagctg ataatactat taagtattgg gcaaggaaat gaaaaaaatt atacgataaa
     1081 atcaaatatc ggaataagta ttgatcttta tagctcgaga agcccggtcg aatcagtttt
     1141 tattagttaa ctgatgccga gtctagcggc taaactcctt ggtagggcta gggatttcag
     1201 gctctggatc tctgaaaaga aacctggaga cagcagttcg aatctgctcc gggctataaa
     1261 tttatttttt taggtccagc ttcaatgatt ctcttagata catccccaaa tttttcaaaa
     1321 ttttggataa ataagttggc tagttttgtt gctgcttgaa tatactcttc ttgattatcc
     1381 cacgtcatta ctggttctaa taactgattt ggtactccag gacattctaa aggtatatct
     1441 aaattaaaaa tatgatgggt tttaaaggaa cttttttcta gagatccatt aataactgca
     1501 gtaaccatag atctcgtata tttcaaatca attcgtttgc ctattccata aggtccacca
     1561 taccaaccgg tatttataag ataaaccttt gtattatgaa gagttatttt gtttcctaac
     1621 atttctgcat attttgtagc atgaagaggc atgaaaggag caccaaaaca ttgtgaaaaa
     1681 gtttctttgg gtagggtaat tccacgttct gtaccagcta atttgctagt atatcctgac
     1741 ataaaatgat acatagca
//
LOCUS       DATAFW010000007         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6167520, whole genome shotgun sequence.
ACCESSION   DATAFW010000007 DATAFW010000000
VERSION     DATAFW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6167520"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..2421
                     /locus_tag="VHJ57_00115"
     CDS             127..2421
                     /locus_tag="VHJ57_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX2486970.1"
                     /translation="MSYDKKDNKKSFNNKGNGKATLKVAEAEQRDIGRKIVRIDPDAA
                     QSLNVMTGDALELSSFGNKSVLLSWPGRDKDRGTGLVRIDGYTRNKLDVGIGDTIEVK
                     KVESKDAKSITFAPTEPLRIIGAEQYLAESLNGQLMTKGDIIPLNVMGQRIDLVVIST
                     DPAGPIIINDATKVTVSEDSAKAVQATQEGKVPSITYEDIGGIRNEIEKVREMIELPL
                     RHPELFKRLGVEAPKGVLLHGPPGTGKTLLAKAVANETNANFYSIGGPEIMSKYYGES
                     EERLRNVFDQAEKNAPSIIFIDEIDSIAPKREEVSGEVERRIVAQLLSLMDGLKSRGK
                     VVVIGATNRVNAIDPALRRPGRFDRELEIGVPDREGRLEILQIHTRGMPLAEDVNLEK
                     LADISHGFVGADLQSLSKEAGIRSLRRILPNIDLSSESIPSDILRKIIVTMNDFMDVI
                     KEMEPSAMREVFVEVPDISWDDIGGLESIKQEMQEAVEWPLKYQEIFTFADAMPPKGI
                     LLYGPPGTGKTLMAKAAANESEANFISIKGPELLSKWVGESEKGVREIFRKARQAAPC
                     IIFFDEIDAIAPKRGGDFGDSHVTERVISQLLTELDGLEVLTNVVVIGATNRPDIIDA
                     ALLRPGRFDRLLYVPPPDRDSRIKIMNIHLKKKPLADDVNIEKLADQAEGYTGADIAS
                     LSSAAVMLALREHVSKYKDPKEAEEHKKELKIHMTHFESAMKKIRPLSTQELNMYRII
                     AEQFGKPDIESKRKGPELQKDFIS"
BASE COUNT          979 a          398 c          533 g          758 t
ORIGIN      
        1 gaacatatcg ctaaaactat ttatcccttt tacaacaatt aaattattct aataaaaaaa
       61 gtaataattt acaagtagag ccatacaata aataaataat agtaaattat tataatatta
      121 ataaaaatgt cttatgataa aaaggataac aaaaaatctt ttaacaataa aggaaatggt
      181 aaagcaaccc taaaagttgc tgaagctgaa caacgggata taggaagaaa aattgttcgt
      241 attgatccag atgcagctca aagtctaaat gttatgaccg gagatgcact agaattatct
      301 tcatttggaa ataaatcagt tctattgagt tggccaggac gggataagga caggggtact
      361 ggcttagttc gtattgatgg ttatacaagg aataaactcg atgtaggtat tggtgatact
      421 atagaagtta aaaaagttga atcaaaggat gctaaaagta taaccttcgc tccaacagag
      481 ccacttcgaa ttataggtgc tgaacaatat cttgccgagt ctttaaatgg acaactaatg
      541 accaaaggtg atataatccc attaaatgta atgggtcaaa gaattgatct agtagtaata
      601 tcaaccgatc cagctgggcc aataattatt aatgatgcta ctaaagtcac agtttctgaa
      661 gatagtgcaa aggcagttca ggccactcaa gaaggaaaag ttccatcaat tacatatgaa
      721 gatattggtg gaataagaaa tgagatagag aaggttagag aaatgataga gctccctctt
      781 agacatccag agttattcaa aagattagga gttgaagctc caaaaggtgt tttattacat
      841 ggtcctcctg gtacaggtaa aactttactt gcaaaagcag ttgctaatga gacaaacgct
      901 aatttctatt ctattggcgg acctgaaata atgagtaaat actatggaga atccgaagaa
      961 agattaagaa atgtattcga ccaagcagaa aagaatgctc cgtctataat ttttatagat
     1021 gagattgact ctatagcacc aaagcgagaa gaagtatctg gagaggtaga aaggagaata
     1081 gttgctcagc ttttatcttt gatggatgga ttaaaatcca gaggtaaagt agtagtaata
     1141 ggcgctacca atagagttaa tgctattgac ccagccctaa gacgtccagg tagatttgat
     1201 agggaattag aaatcggtgt cccagataga gaaggtagac tcgaaatatt acaaatacat
     1261 acaagaggaa tgccccttgc cgaagatgtt aatttagaaa aactagctga tatttctcat
     1321 ggatttgttg gagctgatct acaatctctt tcaaaagaag caggaatcag atcccttaga
     1381 cgaatacttc caaatattga cctgtcatct gaaagtatac ctagtgatat tctccgtaag
     1441 ataatagtaa ctatgaatga ctttatggat gttattaaag aaatggagcc ttctgcaatg
     1501 agggaggtat ttgttgaggt acctgatatc agttgggatg atattggagg attggaatca
     1561 ataaagcaag aaatgcaaga agcggtagaa tggccgctta aatatcagga aatatttaca
     1621 ttcgcagatg cgatgcctcc aaaaggaatt ttgctatatg gaccaccagg aacaggcaaa
     1681 accttaatgg caaaagctgc agcaaatgaa agtgaagcca actttattag cataaaaggg
     1741 cctgagctct tatcaaaatg ggttggagag tctgaaaaag gagtcagaga aatatttaga
     1801 aaagcgagac aagcagcacc atgtatcata ttttttgatg aaatagatgc tatagctcca
     1861 aagcgaggtg gggattttgg agactctcat gttactgaga gagtaattag tcaattactt
     1921 actgaacttg atggattaga agtattaaca aatgttgtgg ttattggtgc aacgaacaga
     1981 cccgatatta tagatgctgc actgttaagg ccgggaagat ttgatagatt actttacgtt
     2041 cctcctccag atcgggattc aagaataaaa attatgaata ttcatctaaa aaagaaacct
     2101 ttggctgacg atgtcaatat agaaaaacta gcagaccaag cagaaggtta tacaggagca
     2161 gatatcgcgt ctctatcctc agctgctgtt atgcttgctt tacgagagca tgtatcaaag
     2221 tataaagatc caaaggaagc cgaagaacac aaaaaagaat tgaaaataca tatgacacac
     2281 tttgagagtg ctatgaagaa gattaggcca ctatctaccc aagaattgaa tatgtatagg
     2341 ataatagcag aacaatttgg aaagcccgat attgaatcaa aaagaaaagg accagaatta
     2401 caaaaagatt ttatatcttg aggatatttt ctttagatat ttctttattt aattaataat
     2461 aattttttca gccatccatt cattattata ggatttacaa tattttttat cgtagtacct
     2521 cgcctgatac agaattgttt taaagatgaa aatctctttc cattttagtt ttatagcttt
     2581 tcctactgta cggccatatg aaatgtaatt taacttttaa tttttagcag ttcacttgac
     2641 aactagagtc tttatttcta atatagga
//
LOCUS       DATAFW010000008         3633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3525206, whole genome shotgun sequence.
ACCESSION   DATAFW010000008 DATAFW010000000
VERSION     DATAFW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525206"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="VHJ57_00120"
     CDS             complement(<1..1757)
                     /locus_tag="VHJ57_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486971.1"
                     /translation="MERNNIKVHTQRVEKMKDATIHIVPHMHYDAVWILNKEDYYFVN
                     IEYILKQAINLSKKFDYKFMLEQTFLLEKIEKDYPHIFSDISDLIKENKIEIAGGQYL
                     LADNMVPNGEVLIREIIEGKKFVKEKLNKDIVVSYGADEFGFNAQWPQILRNSGYKYF
                     LFRRGINISTPSEFFWEGLDGSQILTHWMPLGYRAGLFLDQLPLSLKQLKKYASTNQI
                     LMLSGSGSTPPQPELVDVINQWNKKINGKRVTKSKNEYNMIMSSPLQYFSNLEKQNVK
                     FKVRKGEMYSGKYSAVYPDCTSSRMWLKQGMKEFENSILLLERLDALSWLINIHDENI
                     SDQLHKYWKKILFVAMHDVLPGTGVDEIYNEVREIFENERKSIQRSIFEYLRKISLMT
                     NYENDIFIFNPLSWQVTNWTEIILEFNEGEIKGISCLKSGDQIIEIEILDYSLYQDNT
                     IKKISIGCIISVPPLGYKTLEIVKSDTIDGDTIIAHDTSFKNKGYEIEVNRENAIITV
                     FKSIKNNKKIHYCTGNEIILDEEIGDLYYHRENLKLFKSETGDGIKYGIFKPEKYEVI
                     HRKLKSHIKFKSKYFALRWP"
     gene            complement(1718..2569)
                     /locus_tag="VHJ57_00125"
     CDS             complement(1718..2569)
                     /locus_tag="VHJ57_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX2486972.1"
                     /translation="MSRYLIFSDIDNTLLQSYIIKEGEIYHTEEYGEIKEIVQRLRKN
                     HIPLVLCSSKTRVEQEKIRKYLEIKDPFIVENGGAIYIPENYFPIDLEELGLSINRID
                     KNLVIELGKSYNRIIETLQEIREISSIEFIAVHDLSLPELARKVGITINDAKLMASRE
                     YSETVLEININELDNLKKILNHRGLRILQGTRYNTISSLHDKGSAISILKKLYAMKFE
                     KEYIESIGIGDALNDIPMFENVDKPFLVRNIQNSYTPMKINNLTRVIGIGQTGWKEII
                     LKYILKE"
     gene            2697..>3633
                     /locus_tag="VHJ57_00130"
     CDS             2697..>3633
                     /locus_tag="VHJ57_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486973.1"
                     /translation="MLYSVMQLLEDEEDKKKSSVSLQILKDSIYSNYTQKVYVNSIKM
                     YSRYFDIKDYDNLLTFDKNQTVDMIRKFILYLRNERQLSHGRMNVLFAALKLYYEQND
                     YPFELSWSKLARFKGKNNGKKNQDRTYTREEIQLMLQHSDLRMKCIILVMLSSGIRTG
                     GLAGLRLKDLTYIEQYKLYRIIVYSDSTNDQYYTFTTPECSNYIKLYLEYRKNQGENL
                     VPDSPLIRQKFDTAFDPNDITVTSEDIQERMRYLLKKVGTIKQNKPVTTRKGNDYIYT
                     EQEKIEIRKKRYEVMRCHGFRKFFNTICIESDMNIV"
BASE COUNT         1207 a          588 c          467 g         1371 t
ORIGIN      
        1 ggccatctta atgcaaaata tttactttta aactttatat gggatttgag ttttctatgt
       61 ataacttcat atttttctgg tttgaatatt ccatatttaa ttccatcacc agtttctgat
      121 ttaaataatt ttagattttc ccgatgataa taaagatctc ctatctcttc atctaatatt
      181 atttcatttc ctgtacagta gtgtattttt ttattatttt ttatagattt aaaaactgta
      241 ataattgcat tttctctatt tacttcaatt tcgtatcctt tatttttaaa acttgtatca
      301 tgagcaatta ttgtatcgcc gtctattgta tctgacttta ctatttctaa agttttgtat
      361 cctagtggag gaacggaaat aatacatcct atacttattt ttttaatagt attatcttgg
      421 tataatgaat aatcaaggat ttctatctct ataatctgat ccccactttt taagcaactt
      481 atacctttta tttcaccttc attaaattct aaaattattt ctgtccagtt tgtaacttgc
      541 catgaaagag gattaaaaat aaaaatatca ttctcataat tcgtcattaa tgaaattttt
      601 ctcaaatatt caaaaattga tctttgtata cttttcctct cattttcaaa tatttccctt
      661 acttcattgt atatttcatc aactcctgta cctggtaaaa catcatgcat ggcaacaaag
      721 agtattttct tccaatactt gtgaagctga tctgaaatat tttcgtcatg tatattaatt
      781 aaccaagata aagcatctaa tctttctagg agtaaaatac tattctcaaa ttctttcatt
      841 ccttgtttta gccacatacg tgatgatgta caatcaggat agactgcaga atactttcca
      901 gaatacatct ctccttttcg tactttgaac ttgacgtttt gtttttctaa attagaaaaa
      961 tattgaagcg gagatgacat aatcatatta tattcatttt tagatttagt aactcttttt
     1021 ccatttattt ttttattcca ttgatttata acatcaacta attcaggttg tggaggagta
     1081 gagccacttc cagataacat taaaatttga ttagttgaag catatttttt taattgtttc
     1141 aaggataaag gtaactgatc taaaaaaagt cctgcacgat atcctagtgg catccagtga
     1201 gtaagaattt gggacccgtc aagtccttcc caaaaaaatt ctgagggagt agaaatattt
     1261 attcctcttc taaaaaggaa atatttatag ccagaatttc ttaaaatttg aggccactgt
     1321 gcattaaaac caaattcatc tgccccatat gaaacaacta tatctttatt taatttttct
     1381 tttacaaatt tttttccttc gattatttct cgtattagta cctctccatt tggaaccata
     1441 ttatcagcca acaaatactg tccccctgct atctcaattt tattctcctt tataaggtca
     1501 cttatatctg aaaaaatatg aggataatcc ttttctattt tttctaataa aaaagtttgc
     1561 tctagcatga atttataatc aaatttttta ctaagattta tagcttgttt tagaatatat
     1621 tcaatgttta caaagtagta atcttccttg ttaagaatcc atactgcatc atagtgcata
     1681 tggggaacga tgtgaatagt tgcatccttc attttctcta ctctttgagt atgtacttta
     1741 atattatttc tttccatcct gtttgtccaa ttccaattac tctggttaga ttatttattt
     1801 tcatgggggt gtaactgttt tgaatatttc gtactagaaa tggtttgtca acattttcaa
     1861 acattggaat gtcattaagt gcatccccta ttcctataga ctcaatgtac tctttttcaa
     1921 atttcattgc atataatttc tttaatatag aaattgcaga gcctttatca tgtaaagaag
     1981 aaattgtatt ataccttgtt ccctgtaaaa tacgaagacc tctatgattc aatatttttt
     2041 ttaaattatc aagttcattg atattaattt caagcacggt ttcgctatat tcccttgacg
     2101 ccattagttt tgcatcattt attgttatgc ctacctttcg agctaattct ggtaatgaaa
     2161 ggtcatgtac agcaatgaat tcaatactag atatttctct tatttcttga agagtttcta
     2221 ttattctatt gtacgatttt cctaattcta taactaaatt tttatcaata cggttgatcg
     2281 ataaacctaa ttcttcaaga tctatcggaa aataattctc aggaatataa attgcacctc
     2341 cgttttcaac tataaatgga tctttgatct ctagatattt tcttattttt tcctgttcta
     2401 ctcgagtttt agaggaacat aatactaaag gaatatggtt tttacgtaat ctttgaacaa
     2461 tttccttaat ttctccatat tcttcagtat gataaatttc tccttctttt attatatatg
     2521 attggagaag tgtattatct atatcagaaa atatcaaata ccgtgacatt gttttatttt
     2581 acttttttaa aaatatgaaa tattttatac tttaacgtaa gaaattatat aaaaattttc
     2641 tatatcatgg aaagatttgt tttccccaca aatgtatcgt taaaatatga ctaattatgc
     2701 tatatagcgt aatgcagtta ctagaagacg aagaagataa gaaaaaaagt tctgtcagcc
     2761 ttcaaattct taaagattct atatattcga attatactca aaaggtatat gttaattcta
     2821 tcaaaatgta ttctagatat ttcgatataa aagattatga caaccttctt acatttgaca
     2881 aaaaccaaac ggttgacatg attcgtaaat tcattctata tctaagaaat gaacgccaac
     2941 tatcacatgg aagaatgaac gtcctttttg ctgctctcaa actctactat gagcaaaacg
     3001 attatccatt tgagttgtca tggtccaaac tagcacgttt caaaggaaaa aataatggaa
     3061 agaaaaacca agataggaca tacacaagag aagagattca attaatgtta caacattccg
     3121 atttgagaat gaaatgtata atacttgtaa tgctttcatc tggtattagg acgggtggtc
     3181 ttgctggtct tcgattaaaa gatctcactt acattgaaca atacaaactt taccgtataa
     3241 tagtttactc ggattctaca aacgatcaat attatacctt taccactcca gaatgctcaa
     3301 attacattaa attgtatcta gaatatagaa aaaatcaagg tgagaaccta gtacctgatt
     3361 caccactcat tagacaaaaa tttgatactg cttttgatcc aaatgatatt acggtaactt
     3421 ccgaagacat acaagagaga atgaggtatt tgcttaaaaa agtaggaact atcaaacaaa
     3481 ataaaccagt aacaacaaga aaaggtaacg actatattta cacagaacag gaaaaaatcg
     3541 aaatacgaaa aaaacgatat gaagtgatgc gctgtcatgg attccgtaaa tttttcaata
     3601 ctatatgcat tgaatcagac atgaacattg tat
//
LOCUS       DATAFW010000009         7399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1763589, whole genome shotgun sequence.
ACCESSION   DATAFW010000009 DATAFW010000000
VERSION     DATAFW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763589"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="VHJ57_00135"
     CDS             <1..2074
                     /locus_tag="VHJ57_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX2486974.1"
                     /translation="QLAQFEWFHPISANSEYSDPNLDDLHLRSQKLFQSIDEVVRDLG
                     IKTEIGIMDALMKGKPKNKKELQISEIEQLILKLEEDSKTLVNETSKIINERDSLNRQ
                     LEEYKTLKDTLSVVSNLKIDISRLNDIKLFYQNMFIIKTKDFPEIQRSLSDISLFALK
                     LNDTHSALFIFAKNEDSDRIGKTIRSFDISQIVIPNTFSQNPSEAFQHLSTKIKELTT
                     KKKDIDSNILKISKENNSKILYLRESSQVIREILETLRKPAGLKNFAVIQGYIPTVMA
                     NNFKKLHEKWITIFEHKKPNDFHQIEKDHEHDSEQSKENQEPILFANSRYFRSFEPIT
                     LTQGPPRPNEIDPTPMIALIFPIFYGLMFGDAGQGALLALLGATFRLRGTGNLKKWGT
                     LILASGISAVIAGLIVGEAFGFHIVELPGGEYLRSLGFIGILNAADFTEETVLTILTF
                     SINIGITHLVSAFAFSIYKGIKQGKKYEVLTNRLPTLIMYLAIISLMLAAVGANYKVL
                     EMYTNQNPAPFFSNIAGDWVTVEIVTKISFLILMITMVLQILAVPLGVKLGKVKVHGN
                     VNEELFMTVIEVMLIRLVELFANTISYTRIGIMLLVHVALMGTANNGVEFFLSTGNFA
                     VGIGVAILGNLGVMMIEGLLVYIQALRLHLYEWFTKFYEGTGIPFKKLVPEMLYTSLI
                     WEKKPQQK"
     gene            complement(2633..3301)
                     /gene="tpiA"
                     /locus_tag="VHJ57_00140"
     CDS             complement(2633..3301)
                     /gene="tpiA"
                     /locus_tag="VHJ57_00140"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX2486975.1"
                     /translation="MKGIIIINCKNYLEVAGEKILQLSEIARDISKSNQVQIMIAPPQ
                     NSLFYLSQCVKLPLICQHIDDEKIGATTGFIIPELAKSYGAVGSLINHSEHRIEHKHI
                     QKLVERLGQLNMTSIVCAATSKEVGKLARLNPNMIAIEPPELIGTGKAVSKVNPSIIT
                     KSVKEAARHSENIKVICGAGIVDKTDVKSAINLGSKGILLASGLIKANAWQDKLIELC
                     EGFE"
     gene            complement(3327..4463)
                     /locus_tag="VHJ57_00145"
     CDS             complement(3327..4463)
                     /locus_tag="VHJ57_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HEX2486976.1"
                     /translation="MRITVSAIKADIGAIGGHTRPSDELLDVVKKYIEKTGTKLLIDH
                     YIGYSGDDIHIIMTHKLGTDNEKIHKLAWDAFSEGTKTAKNQGLYGAGQDLLKDSFSG
                     NVRGMGPGVAEIEMEERQSEVFCIFAADKTEPGAFNFPFWRMFVDAGSNTGLLINEKL
                     AGGVTFRIMDVMTGNIADLKMWEDKPELEAALMFPGRYVVHSVYSSEGEQILSASTDR
                     LHNIAGTYVGKDDPIAIVRTQKNFPATEEVGSAFNNPHYVSGNTRGSHHLPLMPVKLN
                     SPASLNYSIPIVECLVFSMHEGRLTGPLDGFGTADWDLQRTWASERAMIMRNQGFIHP
                     ATLVPDELEYNKGYEARMDKLNQKFTSSISNKETQKFSKKGNKK"
     gene            4592..6715
                     /gene="topA"
                     /locus_tag="VHJ57_00150"
     CDS             4592..6715
                     /gene="topA"
                     /locus_tag="VHJ57_00150"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX2486977.1"
                     /translation="MKEIKIHNLKFNSKDKKYSLVICEKPNVANRIAIALGTSQVKKV
                     KIKGNIVFDVLTKDNARYVILSSKGHLYGLKDPNKRGKIFPIFDIYWAPISTSHNKNS
                     KNLIELIRRFAKDTDNFIHACDYDQEGELIGYNILELVCEKAYSNSHRAKLPTLTSDD
                     INKSFITLERTNRNMAYAGMFRHLLDYIYGINFSMAVSRAIKIKNRYSYLTVGRVQSP
                     TLKFIVDRDQQINYFIPTPFWRIKAHFRKDDEVFVFFFEKKRINTLKQVNYIIDKCRG
                     KNGIINNINIYKEYVKPLHPFNIGDLQKESFRLFKYSPVYTLSIAENLYLKALISYPR
                     TNSQILPVSINYRKILNDLEKISDPYKRYVTILLNQNKLSPNNGIKYDPAHPAIYPTG
                     TKPVNLNSSEIKVYDLIVRRFFATFGEPAIFKKTKIDIIVDDNFLFNKEGKNILLKGW
                     IEFYSSFYNYTENSVEYSDLQIGDTLTNIDISKDSELTEPPIYFNYSSLLSKMELEEM
                     GTKSTRGNIIELLIRRNYVSVNNNKIKSTDLGIYLIDFMEKNLPKIISTSLTRTLEHY
                     LADIERGITDRIFILNSIITDLIDSMHYIQKIESVCPIEHQPVRRKNLILIGACPVCK
                     KGKFQIIRSKKTKKRFISCTDYFSDGCTASAPLPQQGLIKNTSKICKECNWPILNVFY
                     PTKRYSKNFCTNINCPTKLIQDIKL"
     gene            complement(6697..>7399)
                     /locus_tag="VHJ57_00155"
     CDS             complement(6697..>7399)
                     /locus_tag="VHJ57_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX2486978.1"
                     /translation="FPEFQMAFSPSTQTLDELYNTAELLANSKFIKRICQYSKKYKIG
                     VVGTFFERNPYNTDKKVFDTAFSTDKNGKIVSIYRKLHLYDAFGFKESLKFSKGDKIP
                     KLITTTIGKMGLMICYDIRFPELSRILTINGSDIIIIPSGWVQGIMKEEHWITMLKAR
                     AMENGVYVIAPNQVGNIFCGRSMVVDPLGSTLSDLGDKRGIEIVEIKKERIDQVRKTL
                     PLLKNRRTDIYNLIS"
BASE COUNT         2557 a         1198 c         1094 g         2550 t
ORIGIN      
        1 tcagctggca cagtttgaat ggtttcatcc aatcagtgct aattctgaat attctgatcc
       61 taatctagat gacctacatt tacgttcaca aaaactcttt caaagtatcg atgaagttgt
      121 acgtgattta gggattaaaa cagaaattgg aataatggat gctcttatga agggtaaacc
      181 aaagaataaa aaagaattac aaatttcaga aattgaacag ttaatcttga aactggagga
      241 agattcaaag actttggtaa atgaaacctc aaaaatcatt aatgaaagag attctttaaa
      301 taggcaactt gaagaatata aaacattaaa agatactcta agtgtagtat ccaatctaaa
      361 gatcgatata tccagactta acgatattaa gttattttat caaaatatgt ttattattaa
      421 aactaaagat ttcccagaga ttcaacgttc gctttcagat atatctcttt ttgccttgaa
      481 attaaatgat acccattctg cattattcat cttcgctaaa aatgaggatt ctgatagaat
      541 aggaaaaaca atacgtagtt ttgacatatc acaaattgtt atacctaata ctttttctca
      601 aaatccaagt gaagcttttc aacatttatc tacaaaaata aaggaattaa ctacaaagaa
      661 aaaagatatt gattctaata ttttgaagat aagcaaagaa aataattcaa aaattctgta
      721 tttgagagaa tcatcccaag ttatacgaga gattttagaa actttaagaa aacctgcagg
      781 attaaagaat tttgctgtta ttcaaggata cattccaact gttatggcta ataatttcaa
      841 gaaattacat gaaaaatgga taacgatatt cgagcataaa aaacctaatg atttccatca
      901 aatagagaag gaccatgaac atgattctga acagtcaaaa gaaaatcaag aaccaatttt
      961 atttgccaat agtcgttatt ttagatcttt tgaaccaata acactgaccc aaggtccacc
     1021 aagacccaat gagatagatc ccactccaat gattgcatta atatttccta ttttttacgg
     1081 attgatgttt ggagatgcag gacaaggtgc attattggct ttgcttggtg ctacatttag
     1141 acttagagga acaggaaatt taaaaaaatg gggtacgttg attcttgcat caggaatatc
     1201 tgcagtaatc gcagggttga tagtaggaga ggctttcgga tttcatattg ttgaactccc
     1261 aggtggggaa tatctgagat cattaggatt cataggaata ttaaatgcag cagactttac
     1321 agaagagact gtcctgacta ttcttacttt ttcaatcaat attggtatta cacacctagt
     1381 aagtgctttt gcattttcta tatacaaggg aatcaaacaa ggcaagaaat acgaagtact
     1441 tactaaccgt cttccgaccc tgataatgta tttggcaatt atttctctca tgttagctgc
     1501 agtaggtgct aattacaagg ttcttgaaat gtataccaat caaaaccctg ctcccttctt
     1561 ttcaaatata gcgggtgatt gggttactgt agaaatagtt acaaagattt cgttcttgat
     1621 tcttatgata actatggtgc tacaaattct tgcagttcct ttgggcgtta aacttgggaa
     1681 agtgaaagtt catggaaatg taaatgaaga attatttatg acagtcattg aagttatgtt
     1741 gattagatta gtagaattat ttgctaatac aattagttat actagaattg gaattatgtt
     1801 acttgtacat gtagctttaa tgggtacagc caataatgga gtagaattct ttttatccac
     1861 cggtaacttt gcagttggaa taggagtagc aatactagga aatctaggtg taatgatgat
     1921 agaaggatta ctagtctata ttcaggcact taggcttcat ttatacgagt ggtttacaaa
     1981 attctacgag ggtacaggaa ttccatttaa aaaattggta ccagaaatgc tatacacaag
     2041 cctgatatgg gaaaagaaac cacaacaaaa ataacataac aagaaccgat ttaattttta
     2101 aaataatatg acctaggaca tctgctcaat atcctaggtc aaggaaattg ttccccttcg
     2161 gtttggtcga tgtatctttt ttatggttaa taggaatatt taaaggtggt ggattgctaa
     2221 aatcatgtat tgaattctat tatcaaaata atttataaat aacatgcggc tataaattaa
     2281 atgatgagtg attttaataa taaaaatttc atattaaacc aagcgctatg gctaggcata
     2341 tctctagcta ttagcattgc tatatctctt atagtaccat tcccctactc attacccatt
     2401 ataattggag ttttccttct tctaagcttt tatatcagac agcgacagtt taagaaacta
     2461 ggattatcaa gtgctactat gtttggtaac agttctgtaa actactattg tatgaattgt
     2521 ggagctaaac ataaccaagt atcatgtcct cgctgcggct caaaaatgaa aagagtggga
     2581 gcataaattt ttatactttg gaatttattt taataaaatt ctgatatcac tattattcaa
     2641 aaccttcaca aagttctatc aatttatctt gccatgcatt tgcctttatt aatccacttg
     2701 ccaaaagtat cccttttgat ccaagattta ttgcactttt aacatcggtc ttatctacta
     2761 ttccagctcc acaaattact tttatgtttt cagaatgtct ggctgcttct ttaacagatt
     2821 tagtaattat cgatgggttg actttgctaa ctgccttccc agttcctatt agttctggag
     2881 gctcaatagc aatcatattt ggatttaatc tagccaattt cccaacttcc ttggatgttg
     2941 ccgcacaaac tattgaagtc atatttaatt gtcccaatcg ttctacaagc ttttgtatat
     3001 gtttatgttc aattctgtgt tcgctatggt ttatcaatga accaactgct ccataagatt
     3061 ttgctaattc aggaataata aagccagtag tagctcctat tttttcgtca tctatatgtt
     3121 gacaaataag aggaagttta acacattgtg ataagtaaaa taaagaattt tgtggaggag
     3181 ctatcattat ttgtacttgg ttactctttg aaatgtctct tgcaatttct gagagttgta
     3241 agattttctc tccagctact tctaggtaat tcttacagtt aattataata attcctttca
     3301 aattattatt ccttttgtac taaaaattat tttttgttcc cttttttaga gaatttttgt
     3361 gtttctttat tgcttatgct tgatgtaaat ttctgattca atttatccat tcttgcttca
     3421 tatcctttat tatattctaa ttcatcaggt actaaagttg caggatgaat aaatccttga
     3481 ttcctcataa tcattgctct ttcagatgcc catgtcctct gtaagtccca atcggctgtt
     3541 ccaaatccat caagagggcc agttagtctg ccttcgtgca tactaaatac taaacattct
     3601 acaattggta ttgaataatt aaggctagct ggtgaattca gttttactgg cattaaaggc
     3661 aaatgatgac tacctcttgt attacctgat acataatgag gattattaaa agcactacca
     3721 acttcttctg ttgctggaaa gttcttttgt gttctaacta ttgctattgg atcatcctta
     3781 ccaacatagg ttccagctat gttatgtaac ctatcagttg atgcagaaag aatttgctcc
     3841 ccctctgaag aataaactga atgaacaacg tatctgccag gaaacatcaa agcagcttct
     3901 aactctggtt tatcctccca catttttaaa tctgcaatat tacccgtcat gacatccatg
     3961 atcctaaaag taaccccacc tgccaatttt tcatttatta agagccctgt attacttcca
     4021 gcatctacaa acattctcca aaatggaaaa ttaaatgcac caggctctgt cttatcagca
     4081 gcaaaaatac agaatacttc actttgtctt tcttccattt ctatttccgc aactccagga
     4141 cccatacctc taacatttcc agaaaaagaa tcctttaaca aatcttgtcc agctccatac
     4201 aatccttggt ttttagctgt tttagttcct tcactaaatg catcccatgc tagtttatga
     4261 attttttcat tatctgttcc taacttatga gtcatgataa tgtgaatatc atcgccgcta
     4321 tatccaatat aatggtctat gagaagtttg gttcccgttt tctcaatata ttttttaaca
     4381 acatcaagta attcatcact aggcctggta tgtccaccaa ttgctcctat atctgctttt
     4441 attgctgaaa ctgttatacg cataatttga ttggattaaa tattgtttat atcttttctg
     4501 ctatgtattt tatcttaatc atctaaaaag ctacctcctt ttattgcatg aaactatatt
     4561 ttgattataa aagttaaata taagaaagtg aatgaaagaa atcaaaatcc ataatttgaa
     4621 gttcaattca aaagataaaa aatattctct tgtaatttgt gaaaaaccaa atgttgctaa
     4681 tagaattgct attgctttag gaacatctca ggttaaaaaa gtaaaaataa aagggaacat
     4741 agtctttgat gtacttacta aagataacgc acgttatgta attctatcct ctaaagggca
     4801 tctgtatggc ctaaaagatc ctaataagag aggtaaaatt tttcctattt ttgatatata
     4861 ttgggcaccg atatccacat ctcacaataa aaattccaaa aaccttatag aattaatacg
     4921 tcgatttgct aaggatactg ataattttat ccatgcatgc gattatgatc aagaaggtga
     4981 attaattgga tataatatat tagaattagt gtgcgaaaaa gcttatagta attctcatag
     5041 agcaaaattg ccaactttga cttcagatga tataaataaa tcttttatta cattagaaag
     5101 aacgaataga aatatggctt atgctggtat gtttagacac ttattagatt atatctatgg
     5161 aataaatttt tctatggctg tttcacgcgc tataaaaata aaaaatagat attcttatct
     5221 aactgttgga agggttcaga gtcctactct gaaatttata gtagatagag atcagcaaat
     5281 aaattacttt ataccaaccc ctttttggag aataaaagct cattttagaa aagatgatga
     5341 agtttttgtt tttttctttg aaaaaaaaag aataaataca ttaaaacaag taaattatat
     5401 tatagataaa tgtaggggta aaaatggtat tataaataat attaatatct acaaagaata
     5461 tgtaaaacca ttacatccct ttaacatagg agaccttcaa aaggaatcat ttagattatt
     5521 taaatattct ccagtttata ctctttcgat tgctgaaaat ctttatttaa aagcattgat
     5581 ttcctatcca aggactaaca gtcaaattct tccagtttca attaactata ggaaaatatt
     5641 aaatgattta gaaaaaattt cagatccata taaaagatat gttaccatat tgttaaatca
     5701 gaacaagctt tctccaaata atggtataaa atatgatcct gctcatccag ctatttatcc
     5761 tactggaaca aaaccagtaa atctaaattc ttcagaaata aaagtttatg atctcatcgt
     5821 aagaaggttt tttgctacat ttggtgaacc tgcaattttt aaaaaaacta aaattgatat
     5881 tatagtagat gataattttt tatttaataa agaaggaaaa aatatcctct taaaagggtg
     5941 gattgaattt tatagttcat tttataatta tactgagaac tctgttgaat actcagacct
     6001 tcaaatagga gatacattga ctaatataga tattagtaaa gattcagagt tgactgaacc
     6061 tccaatttat ttcaattatt catctttatt atcaaaaatg gaattagaag aaatgggcac
     6121 aaaatctacc agaggaaata ttatagaatt actaatacga agaaattatg tatctgtaaa
     6181 taataataag atcaaatcaa ctgatcttgg aatatattta atagatttta tggaaaaaaa
     6241 tctcccaaag ataatttcta ccagtttgac caggacttta gaacactatt tagcagatat
     6301 tgaaagagga attacagata gaatatttat actaaatagt ataattactg atctaattga
     6361 ttctatgcat tatattcaaa aaatagaatc tgtatgtcca atagaacatc aaccggttag
     6421 acgcaagaat ttgatattga taggagcttg tccagtttgt aaaaaaggaa agtttcaaat
     6481 tataagatct aaaaaaacca aaaaacgatt tatctcatgc accgattact tttccgatgg
     6541 ttgtactgct tctgccccac ttccacaaca aggtttaatt aagaatacga gtaaaatctg
     6601 caaagaatgc aattggccaa ttctaaatgt gttttatcca actaagagat acagtaaaaa
     6661 cttttgtaca aatattaatt gccctacaaa attaattcaa gatattaaat tataaatatc
     6721 agtccttcga ttctttaata aaggtagtgt cttcctgact tggtcgattc tttctttttt
     6781 aatctctaca atttcaattc ctctcttatc gcctaaatca ctaagagtag atcccaaagg
     6841 atctaccacc atacttcttc cacaaaatat atttccaact tgatttggtg ctattacata
     6901 tactccattt tccatagctc ttgctttgag catggtaatc caatgttctt ccttcattat
     6961 accctgaacc catcccgatg gtattattat tatatctgaa ccattaatag taagtattct
     7021 agagagttct ggaaatctta tatcataaca aatcatcaaa cccattttac ctattgtagt
     7081 tgttattagt tttggaattt tatctccttt actaaacttt aaagactctt taaaaccaaa
     7141 ggcatcataa agatgcaatt ttctataaat cgaaacgatt tttccatttt tatcagtact
     7201 aaaagcagta tcaaaaactt ttttatctgt gttataagga tttctttcaa agaatgtacc
     7261 tactacccct attttatatt ttttagaata ctgacatatt ctctttataa atttactatt
     7321 tgctagaagt tctgcagtat tatagagttc atcaagtgtt tgagtagatg gagaaaaagc
     7381 catctgaaat tccggaaaa
//
LOCUS       DATAFW010000010         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1763960, whole genome shotgun sequence.
ACCESSION   DATAFW010000010 DATAFW010000000
VERSION     DATAFW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763960"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VHJ57_00160"
     CDS             complement(<1..669)
                     /locus_tag="VHJ57_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX2486979.1"
                     /translation="MGIPEKIKEIEDEIHKTQINKATEFHIGILKAKIAKLKRELEEN
                     RHGKTVSSGKGSSGYDVRKEGDATVVLIGFPSVGKSTLLNCLTNANSKIASYQFTTLT
                     AIPGMMEYRGAKIQVIDLPGIIKGAASGKGMGKKVLSVARNADLVLIIVDVFQPKHFS
                     ILVKELSEIGIKVNKNPPNVHIHKTEKGGISANIHQDSQISESMVKEMCRIYGIHNGR
                     ITIRD"
     gene            complement(719..1270)
                     /locus_tag="VHJ57_00165"
     CDS             complement(719..1270)
                     /locus_tag="VHJ57_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486980.1"
                     /translation="MSEFLTRYPKKMNFLKGLQESIKSEELQFDHLSLLVKIKKDEII
                     RTLILEGFSKVKFENKKPGQIGNGFSKKLKKPWEIHVRLLDMQQGLIAIQGEVEISRR
                     YIQHIRSVRAPVIYEIESILKKHRIEYKIWNDKVRDYITNIIENHHIILNSPKLPAMP
                     WLLMIIFGSIIGSLHGLKLFGIF"
     gene            complement(1325..2086)
                     /gene="cofE"
                     /locus_tag="VHJ57_00170"
     CDS             complement(1325..2086)
                     /gene="cofE"
                     /locus_tag="VHJ57_00170"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2486981.1"
                     /translation="MIIVKGIKIKTKKKMFNLFETLISAKYEFKDGDILVLSSKFVSM
                     SEGSIVDLRKIRPSSKAKNLAKNYQMNPSFVEVVIRESDVIFGGLPGFLLTIKNGILA
                     PNAGIDKSNIPSNYVICLPIDPFISAENLRMEFLIRSGIKVGIVISDSRLMPTRIGTT
                     GVAIGCSGIEPVEDQRGKKDLFGHIMKFTLKATADALATIGTFVMGESNEAVPLVVIR
                     GSNISFTDRKLCWKDFAIDYQTDLYFRGIQNFAIK"
     gene            2169..3446
                     /locus_tag="VHJ57_00175"
     CDS             2169..3446
                     /locus_tag="VHJ57_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX2486982.1"
                     /translation="MGMTITEKILARASDKKSVQPDDVVIATIDKVMVHDVSGPGVIK
                     VFSEWEKKGIILDHIWDPDRVWISEDHFVPASDRISANNITLLTNWTKKYNIKKHFKY
                     GLGQYGICHTLSHEEGLVLPGEIYVGGDSHTNTTGAMGSLAVGLGHTDIAYVLMNGKI
                     WFKVPETILFKINGHKPEYIMAKDIILKIIGDIGTDGANYKAMQFTGEAVDKMSMEER
                     LTLTNMSTEAGAKNGIIEPDEITFDYLRTRTDKEFLPVKGDFDAIYSDTFTYDTKNLE
                     PVIAKPFSPENICAVGDIEGRAIDKAYIGSCTGAKLEDLRMAAKILKGKHVKIRTEVL
                     PAAQSIYLKAIKEGLVTIFLEAGVVVGPPTCGACCGAHMGVLAKDEICISTTNRNFPG
                     RMGDVESQTYLASPLVVAAAAVNGKITDPRNEM"
     gene            3446..3937
                     /gene="leuD"
                     /locus_tag="VHJ57_00180"
     CDS             3446..3937
                     /gene="leuD"
                     /locus_tag="VHJ57_00180"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019913.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2486983.1"
                     /translation="MNRLKGKIHKYNRDNIDTDVIIPGPYLKIHDHKELGKHAMEGLD
                     PNFSEKICEGDFLLTGKNFGCGSSREHAPIALHQVGIRAILSPSFARIFYRNAVDGGY
                     LLPIEIDPALIEKISDKDEIEIDLNTNTIINITSSEKFNIKPFPEIISKIVKAGGLLY
                     FKE"
BASE COUNT         1306 a          636 c          663 g         1333 t
ORIGIN      
        1 atctctgatt gtaattctac cattatggat tccatagatt ctacacattt ctttaaccat
       61 tgattctgaa atttgtgaat cttggtgaat attggctgaa atccctcctt tttcagtttt
      121 gtgtatatga acattagggg ggtttttgtt aactttaatt cctatctccg ataattcttt
      181 tactaatata gaaaaatgtt ttggttggaa tacatcaaca ataattaata ccaaatctgc
      241 atttctagca actgagagaa cttttttacc catccctttt ccggaagccg caccttttat
      301 gattcctggt aagtcgatga cttgtatttt tgctcctctg tactccatca tacctggaat
      361 agctgttagt gtagtaaact gatacgatgc aatctttgaa ttagcattag ttaaacaatt
      421 taaaagtgta gattttccaa cacttggaaa tccaatcaga actacagtgg catcaccttc
      481 ttttctaaca tcatatccag aactaccttt tccgcttgag acagtctttc catgcctatt
      541 ttcttcgagt tcccgtttaa gtttggcaat ttttgctttt aaaattccaa tgtggaattc
      601 cgtagcttta tttatttgtg ttttgtgaat ttcatcttct atttctttga ttttctctgg
      661 aattcccatt gtttgattgt aaatactatc aaatgaagat cataaaagaa tttcttcttt
      721 aaaaaatacc aaagagtttt agaccatgta aagaaccgat tatggaacca aagattatca
      781 ttaataacca aggcatagct ggtaattttg gagaattaag aattatgtgg tgattttcaa
      841 taatattagt tatataatca cgtactttat cattccagat tttatattca attctatgtt
      901 tctttagaat agattcaatt tcataaatta ctggagcgcg gactgatcta atatgttgaa
      961 tatatcttcg tgaaatttct acttcgcctt gaattgcaat caatccctgt tgcatatcca
     1021 ggagtcgaac atgaatttcc caaggttttt ttaatttttt tgaaaatcca ttacctattt
     1081 gtccaggttt cttattttca aactttactt ttgaaaatcc ctccaaaata agcgttctaa
     1141 taatttcatc tttctttatt ttaactaaaa gacttaggtg atcaaactgt agttcttcag
     1201 actttatcga ttcctgcaat ccttttaaaa agttcatttt ttttgggtat ctcgtaagga
     1261 attcagacat ttggttattg aatttttgat gttatagtta tattaaaacg ctatgaaaat
     1321 aaaattattt aatggcaaaa ttttggatac cacgaaaata aagatctgtt tgataatcaa
     1381 tagcaaaatc tttccaacat aactttctat cggtaaagga aatatttgaa cctcgtatta
     1441 ccactagtgg aactgcctca ttgctttcac ccattacaaa ggtacctatt gttgctaaag
     1501 catcagcggt tgctttaagt gtaaatttca taatatgtcc aaaaagatct ttttttcccc
     1561 tttgatcttc tactggttct atgccagaac aacctatagc taccccagta gtaccaatcc
     1621 gtgtaggcat taatctgcta tctgaaataa ctattccaac ctttatgcct gatcttataa
     1681 gaaattccat tcgtagattt tcagcagata tgaatggatc tatgggaaga catataacat
     1741 aattcgaagg tatattggat ttatcaatgc ctgcatttgg ggctaaaata ccatttttta
     1801 ttgttaataa gaatccaggt aaccctccaa atataacatc agattctcta attaccactt
     1861 cgacaaagct tggattcatc tgataatttt tagcaagatt tttggctttg gagctaggtc
     1921 taattttccg aagatctact atcgatcctt cactcataga aacgaattta cttgataaaa
     1981 caagaatgtc cccatcttta aactcatact ttgcggatat aagagtttca aacagattaa
     2041 acattttttt tttagtttta attttaatcc cttttacaat tatcaatatt actctaccag
     2101 aataaagagt tattagcttt ttagaaggtt tttattcgcg aattggtaac tttatttagt
     2161 ataaagaaat gggaatgacc attaccgaaa aaattttagc tagagcctct gataaaaaaa
     2221 gcgttcaacc agatgatgta gtcatagcta ctatagataa agttatggtt catgatgtat
     2281 ctggtcctgg tgtaattaaa gttttttctg aatgggaaaa aaagggtata atacttgatc
     2341 acatctggga ccctgatcga gtatggataa gcgaagacca ttttgttcct gcctctgata
     2401 ggatttcagc aaataatatt accttattaa ctaattggac taagaaatac aatataaaaa
     2461 aacattttaa atatggttta ggtcagtatg gaatttgtca tactttatct catgaagaag
     2521 ggttagtttt acctggggag atttacgtcg gtggtgactc tcatacaaat actacaggtg
     2581 caatgggttc ccttgctgtc ggattaggtc atacagatat cgcatatgta ctaatgaatg
     2641 gaaaaatatg gtttaaagta cctgaaacaa tccttttcaa aataaatggc cataaacctg
     2701 aatacataat ggctaaagat ataatattga agataatagg tgatatcggt accgatggtg
     2761 ctaattataa ggctatgcaa tttactggag aagcagtaga taaaatgtct atggaagaaa
     2821 gattaacact tactaatatg agtacggaag caggagctaa aaacggaatt atagagcctg
     2881 atgagattac atttgactat cttaggacac gtactgataa ggaatttctt cccgtaaaag
     2941 gagatttcga tgcaatatat agtgatacat ttacgtacga tactaaaaac ctggaacctg
     3001 ttatagctaa gccattttca cctgaaaaca tatgtgctgt aggtgatata gagggtagag
     3061 caatagataa agcctatatc ggatcttgta caggggctaa attagaagat ttaaggatgg
     3121 cagcaaagat ccttaaagga aaacacgtta aaattagaac agaggtttta cctgctgctc
     3181 agtcgatcta cctaaaagca ataaaagaag gattggtaac tatatttttg gaagcaggtg
     3241 ttgtagttgg tcctcctact tgtggggctt gttgcggtgc acacatgggt gtacttgcaa
     3301 aagatgaaat ttgtataagt accactaaca gaaatttccc tggtagaatg ggagatgttg
     3361 aatcacaaac atatttagca tcgcctttag tagttgccgc agcagctgta aatggaaaaa
     3421 taaccgaccc aaggaatgaa atgtaatgaa tagacttaag ggaaaaattc acaaatacaa
     3481 tagagataat atagatacag acgtaataat cccaggacct tatctaaaaa tacacgatca
     3541 taaagaattg ggaaaacatg caatggaagg actagatcct aatttttccg aaaaaatatg
     3601 tgaaggagat tttttattga ctggtaagaa ttttggttgt ggatcaagcc gagagcatgc
     3661 tcctatagca ttacatcaag taggtatcag ggcaatactt tctccttcct ttgccagaat
     3721 tttttataga aatgcagtag atggaggtta tcttttacct atagaaatag atcctgctct
     3781 tattgaaaaa atatcagata aagatgagat agaaattgat cttaatacta atactatcat
     3841 taatataaca tccagtgaaa aatttaacat caaacccttt ccagaaataa tctcaaaaat
     3901 agtaaaggca ggaggattac tttactttaa ggaatagg
//
LOCUS       DATAFW010000011         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4759869, whole genome shotgun sequence.
ACCESSION   DATAFW010000011 DATAFW010000000
VERSION     DATAFW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4759869"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..767
                     /locus_tag="VHJ57_00185"
     CDS             354..767
                     /locus_tag="VHJ57_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486984.1"
                     /translation="MRKKYTILLYTAAATTLIAGILHINKVIDTISSSEAINNTDILF
                     LVGGAAQVFWVIPIIRKWGKIWYSIGIAGTAVFMLLWVITRIPENPITGKAGPISGEA
                     ITIQVLQIAFIILSIILLIKSTKAKNSRIKNIQDT"
     gene            822..>2178
                     /locus_tag="VHJ57_00190"
     CDS             822..>2178
                     /locus_tag="VHJ57_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2486985.1"
                     /translation="MKVLRNSKEKKFHDTSLNLVKIDFSLKREVLYVVVGAIIGAITM
                     ILPGTIYEFITGLPYYTSWIIFGHVIGVYSSYAATAGFIIHLITAVSIGIVVGIFLYK
                     TNILNISKPINGLIYGIFAGTIIFVVWAIPVNEFILIPENIRTTADIDPILTEQEVSQ
                     KVQSNKLNVFLNSLFLNLLFGITVGLVSSFLSIKFGARYKCNICNVSFSRINVIKKHL
                     QSVHRRDPIQQKKIAILGGGFAGVEVLTKLQKEFQNDIRVDITLISKDNFFLFTPMLH
                     EVSSGMIETRHVASPLRAFCKRARFIESEVKSIDLENKRIIFTNPILDARIRNSIREE
                     QVMKLDTYLLSNNDHLASKSPLRNSTNNTKNYGDYTKSLDYDYLVVALGSNTNFFGNK
                     NLEKASFTMKSLYDAFSIRSHIIDTLEQTDMLLFEEKKDVVIDLKIKNSTSKIFHSIY
                     RD"
BASE COUNT          861 a          264 c          324 g          729 t
ORIGIN      
        1 ccaatattaa ctacgcaatt atactttgaa ggagatcctt atcttactga cagagaagat
       61 aaatcattga taatgaaagt tacagatgat aagaatggaa ctaaaaaagc caattttgat
      121 tttgtaattg aatattatga cgaatacgaa aaataaaaca taaagatata catttttaaa
      181 atacttaagg atgctatctc ttgtctagta actatcatgt ttaaaattat ttaatatata
      241 attgaaaaaa atttaaagtt ataaattagt aatatactat agttacaaat aaatattttt
      301 gtataatcat tatataaaat aaattacaac gtgattatga ggtttttttg ttaatgcgaa
      361 aaaaatatac tatattacta tatacagctg ctgcaactac tttgattgca gggatactgc
      421 atataaacaa ggttatagac actatcagta gtagtgaagc tattaataac acagatattt
      481 tatttttagt tggaggagca gctcaagtat tttgggttat tcctatcatt agaaaatggg
      541 gtaaaatatg gtactccata ggcatagcag gaacagcagt ctttatgtta ttatgggtaa
      601 ttactagaat acctgaaaat ccaataacag gaaaggccgg cccgataagt ggagaagcaa
      661 taacaataca agtattacaa attgcgttta ttatcttgag tataatcttg ttaattaaaa
      721 gtacaaaagc taagaatagc agaattaaga atattcaaga cacataatat tatatagaga
      781 atttatgttt tatattaaaa aactaagaca aatgaattaa aatgaaagta ttaagaaatt
      841 ccaaagaaaa aaaatttcat gatactagtc ttaatttagt aaaaattgat ttttctctaa
      901 agagagaagt attgtatgtt gtagtaggtg ccataattgg tgctataaca atgattcttc
      961 ctggaactat ctatgaattc ataaccggtt taccatacta tacatcctgg attatatttg
     1021 gtcatgtaat aggtgtttac tcttcgtatg ctgctactgc aggatttata attcatttaa
     1081 ttacagcagt ttcaattgga atagtagtag gtatattttt atacaaaaca aatattttga
     1141 atataagtaa acctataaac ggtcttattt atggaatttt tgcaggtaca ataatatttg
     1201 ttgtatgggc tattccagta aatgaattta ttttaattcc agaaaatatt agaactactg
     1261 cagatatcga tccaatatta acagaacaag aagtatctca aaaagtgcaa agcaataaat
     1321 taaatgtttt tctgaattct ttatttctca atctcttatt tggaattaca gtcggccttg
     1381 tatcttcttt tttatctata aaatttggtg cacgatataa atgcaatatc tgcaatgtat
     1441 ccttttccag gataaatgtt ataaaaaaac atttacaatc agttcatcgt agagatccaa
     1501 tacaacaaaa aaaaattgca atacttggag gaggttttgc tggagttgaa gttctcacaa
     1561 aactacaaaa agaatttcaa aacgatataa gagtagatat tactctaata agcaaagata
     1621 atttctttct tttcactccc atgttacatg aagtatcttc tggaatgatt gagacaagac
     1681 atgtagcatc accattgagg gcattttgta agagagctag atttatagaa tctgaagtta
     1741 aatcaataga tttagaaaat aaaagaatta tattcactaa tccaatttta gatgcaagaa
     1801 ttcgtaatag tataagagaa gaacaagtta tgaaattaga cacttatttg ctatctaata
     1861 atgatcattt agcttcaaaa agtccgttaa gaaattcgac aaataataca aaaaattatg
     1921 gagattatac caaatcatta gattatgact atctagttgt agcattgggc agtaacacaa
     1981 atttctttgg caataaaaac ttggaaaaag catcatttac aatgaaaagt ctttatgatg
     2041 cattttctat tagaagtcat attattgata ctttagaaca gacagatatg ttactatttg
     2101 aagaaaaaaa agatgtagta atagatctaa aaataaaaaa tagtacaagt aaaatttttc
     2161 attcaatata tcgagata
//
LOCUS       DATAFW010000012         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_179268, whole genome shotgun sequence.
ACCESSION   DATAFW010000012 DATAFW010000000
VERSION     DATAFW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179268"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="VHJ57_00195"
     CDS             complement(<1..1465)
                     /locus_tag="VHJ57_00195"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2486986.1"
                     /translation="MPSIFLTIIALVSVNSHLWVASAIHHFYIELFGTLLGGILAFYY
                     ISRAQTLNDKFSLFIGIGFLVTALIDFLHVLVSYVYMDNSIFLKYFIPQTWFAGRLFL
                     SAMLAIAMIKYSFFSSITPVTKQQISLKLNIEDKISKPLLFYLIIVTLFASSVAVSSL
                     FVVFPFSVIDNIPLHRPYEIFPLVLFSLSLYYFYKNKIYKNKDVFYTSIIVSIVFDIF
                     GQIIMSYSGHHFDTAHNMAHVLKDASYFINIIGLALSGIQYNTRLKETNEILRIQYQK
                     VKESEKMKDEFINIAAHELRTPIQPILGLTDIMYSKIKDEEQRELLDVIIRNAKRLKR
                     LTDNVLDVTKIESQSLVFNKEKLNLNSVISDVLKEYVNKNISEEMPKIVYDFKPKNGI
                     MVEADKDRLSQVIHNLLDNALKFTLYNQMIFVNVALNKDKEQVTVSVKDTGEGISKEI
                     LPNLFSKFKTHSNTGTGLGLYICKNIIESHGGRIWGEN"
BASE COUNT          684 a          278 c          268 g          704 t
ORIGIN      
        1 tattttctcc ccatattctg ccaccatgtg attctataat attcttacaa atatagagtc
       61 ccaatccagt gccagtatta gaatgagttt taaattttga aaacaaattg ggcaatatct
      121 cttttgaaat gccttcgcca gtatccttca cactgactgt aacttgttct ttgtctttgt
      181 tcaatgctac attaacaaat atcatttggt tgtataaagt aaattttaag gcattatcta
      241 aaaggttgtg gatgacttga gataatctgt ctttatctgc ttcgaccata ataccatttt
      301 tcggtttaaa atcgtatact atttttggca tttcctctga aatatttttg tttacatatt
      361 cttttagaac atcagatata acactattca gattcaattt ttctttatta aaaactaaag
      421 attgactttc gatttttgta acatccaaga cattatcagt aagccgtttt aatctttttg
      481 catttcttat tataacgtct aataattctc tttgctcctc atctttaatt ttagaataca
      541 ttatatctgt caacccaagt ataggttgta taggagttct taattcgtgg gcagctatat
      601 taataaattc atccttcatt ttttctgatt ccttcacttt ttgatattgg atacgcagta
      661 tttcattggt ttcttttaac ctagtattat attgaatacc tgataatgcc aatcctataa
      721 tattgataaa atagcttgca tcttttaaga catgcgccat attatgggca gtatcaaaat
      781 gatgtccgga ataagacatt attatttgtc caaaaatatc aaatacaata gaaacaatta
      841 tactagtata gaaaacatct ttatttttat agattttatt tttatagaaa taatagagtg
      901 acaatgaaaa taatactagt ggaaaaattt catatggtct atgtaaagga atattgtcta
      961 ttactgaaaa tggaaacaca acaaagagtg aactgacggc tacactagaa gcaaataaag
     1021 taactatgat caagtaaaat aaaaggggtt ttgatatttt atcttcgata tttaatttta
     1081 aagatatttg ttgttttgta actggagtaa tagatgaaaa aaaagaatac tttatcattg
     1141 ctatagccaa catagcactt aaaaataacc gccctgcgaa ccaagtttgg ggtatgaaat
     1201 acttgagaaa tatactatta tccatatata catatgaaac tagaacatgc aaaaaatcga
     1261 tcaaagcagt tacgagaaag ccaataccaa taaatagact gaatttgtca tttaatgttt
     1321 gagcacgaga tatataatag aatgctagta ttcctccaag taatgtacca aataattcga
     1381 tataaaaatg atgaatagcg gatgctaccc ataaatgaga attgactgaa actagagcaa
     1441 ttatggttag aaaaattgaa ggcaatatga cgtatttaaa tagatgactc tccctattat
     1501 gaccccaata agaaaagaga gatgtcaaat tggacttaat cttctctttt tttctttatt
     1561 taaaaataaa aaaactacaa ataacttgaa aaaaccatag atattttttt gtattaattt
     1621 gttatggtta tacatgtagg acaaactaga aaacatcctt gagcttataa gaatatctat
     1681 cctttgtgcg aaattatgac gttctcagtt atttgttatt taagatataa gatatctatt
     1741 ttaaacttta aaaatgatat acattcaaaa tatgactaga taaatcactg gggacaaggt
     1801 tcgatgctct ctatcaggat atttaatgta atagttgttt aatttattaa tttttttaca
     1861 gtagttgtaa ataccttttt atcaactggt tttctcataa tttggtcttt tgatactcca
     1921 ggaactatac ttaa
//
LOCUS       DATAFW010000013         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_179299, whole genome shotgun sequence.
ACCESSION   DATAFW010000013 DATAFW010000000
VERSION     DATAFW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179299"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1270
                     /locus_tag="VHJ57_00200"
     CDS             143..1270
                     /locus_tag="VHJ57_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HEX2486987.1"
                     /translation="MIEKYKDITVVNGKNSIEILRSNNPIFVCVIAYTETSEIPGITV
                     AGANKDLIKYTPSADAEFLYYGKCKCIDGVPATPDGKPTPALITKTMLDISNIPFIVV
                     DSGVKVKPDIPLFSFNLQSGKNILKDIAMELEEVEKAYRYGLMLGKQLSKSNDFVIVG
                     ESIPGGTTTALGVMLSIGIDAENKMSSSMPQNPHTLKNMIVRKSMKKNNITFGSLKDK
                     PFAAISLIGDPMIPSVIGVAQGVIDNGGRVMLAGGTQMCTILSILKSMNVKTDKIFIG
                     TTKYIFNDTNADFTNLVLSIDNNVGIFVLDLHLIESTKPGLKSYANGFVKEGVGAGGL
                     SIATLINANGKLNGETLLKYIENEYEKNIERNILNEINTSL"
     gene            complement(1298..>2477)
                     /locus_tag="VHJ57_00205"
     CDS             complement(1298..>2477)
                     /locus_tag="VHJ57_00205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isomerizing glutamine--fructose-6-phosphate
                     transaminase"
                     /protein_id="HEX2486988.1"
                     /translation="LEYTDKAIFLDNHDIVILDHNRVNLFDFNGNNVYRPITQVAWEL
                     GDIDKGKYAHYTLKEIHQQKNSIPLAFQIDQISLQKAVDLISSSKNIFITGSGTSYHS
                     ALLAKYLFYKFAKIRTEIIMSSEFEYMSAPIDENSILIAISQSGETADVLNSVKIANE
                     HKAKILSIVNISTSSLSRLSDHSVSINCGPEMGVAATKSFTSQLATLYSIIDTLCVSE
                     KSLDISTSMLGNALNEILINDSYLEKIADDIKSVQDIYILGKSVHYPIALEGSLKIKE
                     LAYIHAEGVAAGEIKHGPLAMIEPNTIVIVLNPEDETYYDTLHSIHEMKSRGAKIIGI
                     SNKRNDIYDYFIKLPLVNENLYPLIEIIPIQILSYYLALKKNANPDYPRNLAKSVTVK
                     "
BASE COUNT          897 a          336 c          414 g          830 t
ORIGIN      
        1 aataatatga ttgtgtatcc aggtcataat tatggataca aaccaacttc tacgatagaa
       61 gaagaaaaga aaaataatta tgttctaatt cctagatcaa agaatgaatt tataaagttt
      121 attactcatg gggaataaca aattgataga aaaatataaa gatattacgg tagtaaacgg
      181 caaaaatagt attgaaatac tacgatccaa taatcctatt ttcgtttgtg taatagcata
      241 tactgaaaca agtgaaattc caggtattac tgttgctggg gctaataaag atttaataaa
      301 atatacacct tcagctgatg ccgagtttct ttattatggt aaatgtaaat gtatagatgg
      361 tgtacctgca actccggatg gaaaaccaac ccccgctctg ataacaaaaa ccatgttaga
      421 tattagtaat attcctttta tcgtagtaga ttcaggagta aaagtaaaac cagatatacc
      481 attattttca tttaatttac aatcagggaa aaatatcttg aaggatatag caatggagtt
      541 agaagaagta gaaaaagcct atagatatgg attaatgctc ggaaaacaac tttctaaaag
      601 taatgatttt gtaattgttg gagaaagcat tcctggggga actaccactg cacttggggt
      661 tatgctttct ataggtatag atgcagaaaa caaaatgagt agtagtatgc cacaaaatcc
      721 acatactctc aaaaatatga tagtaagaaa aagtatgaaa aaaaataata taacttttgg
      781 atcattaaaa gataaacctt ttgctgctat ctctttgata ggagatccaa tgatcccttc
      841 agtgataggt gttgctcaag gggttattga taatggtgga agagtaatgt tagcaggagg
      901 aactcaaatg tgtactattt tatccatttt aaaaagtatg aatgttaaaa ctgataaaat
      961 ctttatcggt accacaaaat atatttttaa tgatactaac gcagatttta caaacctagt
     1021 cttatctata gataacaatg tagggatttt tgttttggat ttacatttga ttgaatcaac
     1081 aaaaccagga ttaaaatcat atgcaaatgg ctttgtaaaa gaaggagttg gtgctggcgg
     1141 gctatcaata gcaacactga taaatgcaaa tggaaaactt aacggagaaa ctttgttgaa
     1201 atatattgaa aatgaatatg agaaaaatat tgaaagaaac atattaaacg aaattaatac
     1261 ctcattatga aaaaataaac taaataaaaa attaaattta ttttacagtt actgatttag
     1321 ctaagtttct aggatagtca ggattagcat tcttttttaa tgctagataa taggataata
     1381 tttggattgg gataatttca attaggggat acaaattctc attaactaat ggtaatttga
     1441 taaaatagtc ataaatatca tttcttttat ttgaaattcc aataatcttt gcacctctcg
     1501 atttcatttc atgtatacta tgtaatgtat cataatatgt ttcatcttct ggattaagaa
     1561 ctatgacgat tgtatttggt tcaatcattg ccaaagggcc atgtttgatt tcgccagcag
     1621 ctacaccttc tgcatgaata taggcaagtt ctttaatttt aagagatccc tctaatgcaa
     1681 ttggataatg aacggatttt cctagaatat atatatcttg gacagatttt atgtcatcgg
     1741 ctattttttc taaatatgaa tcatttatta gaatctcatt tagagcatta ccaagcatac
     1801 ttgttgatat atctaatgat ttttcactta cacataaagt gtctataata gaatataatg
     1861 tcgccaattg tgaggtaaag ctttttgttg cagcaacgcc catttctggt ccacagttta
     1921 tggaaacaga atgatcgctt aatcgagata aagaagaagt agatatattg acgatggaaa
     1981 gaatttttgc tttatgctcg ttagctatct taacagaatt taaaacatct gctgtttcac
     2041 cactttgaga aatagcgatt agtattgagt tttcatcaat aggtgcagac atatattcaa
     2101 actcacttga cattattatt tccgttctaa tcttcgcaaa tttatagaag agatattttg
     2161 ctaataatgc cgaatgatag cttgttccac tacctgtaat aaaaatattt ttagaggaag
     2221 aaataagatc tacggctttt tgaagagata tttgatcaat ctgaaaagcc aaaggtatag
     2281 aatttttttg ttgatgtatt tctttgagtg tatagtgtgc atacttacct ttatctatat
     2341 ctcctaattc ccaagcgacc tgagttatag gtctatatac attattaccg ttaaaatcga
     2401 ataagtttac acgattatga tctagaataa ctatgtcatg attgtcaaga aagatagctt
     2461 tatcggtata ttctaag
//
LOCUS       DATAFW010000014         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5993432, whole genome shotgun sequence.
ACCESSION   DATAFW010000014 DATAFW010000000
VERSION     DATAFW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5993432"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VHJ57_00210"
     CDS             complement(<1..677)
                     /locus_tag="VHJ57_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX2486989.1"
                     /translation="MYYNLASLNNNNNNNNINNFKQEIEVKGHLIDSMILTNIFDNIM
                     DLKGEFRVLEFTVGKNKTDPSYARLIVEGKDKNHLNQILESIYRAGAQSITTQSVLLE
                     PAPKDMVMPDLFYSTTNKPTQIFYDGIWMNVKNIMMDKCIVVDTYNKIAECKMIRDIK
                     KDDMIVIKERGIRIIPDERPRQGVDIFQFMSSTTSSERPTQHIIKKVAYDIYEVAARQ
                     KGKIIVVG"
     gene            complement(707..1033)
                     /locus_tag="VHJ57_00215"
     CDS             complement(707..1033)
                     /locus_tag="VHJ57_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486990.1"
                     /translation="MQYFTALKLGEKRVKQSQDLLNLYTGNAAMPALALKDTKTNVWV
                     PVGEENLIAVFKEASGYVIVICDKNGIAKTIAQWFSEEDKNNIVSRIKKDHSLSEYTG
                     KLSLPI"
     gene            complement(1046..1600)
                     /locus_tag="VHJ57_00220"
     CDS             complement(1046..1600)
                     /locus_tag="VHJ57_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2486991.1"
                     /translation="MNDYKFSNLIDYILKLKTIKRTGWISKAKILKAESVADHSYSLT
                     ALSMVFSDLLGLDTEKVMKLCIIHDLAESIIGDYMPEEISVLEKHTKEDNAMKVIISS
                     FPSKIAILYSELWKEYTLNQTKEVHLVKQLDKIEMFLQANQYFRNGYSFEFLSQFLKV
                     SGNIEGSDFHPTIANILKHLDKNS"
     gene            complement(1908..2483)
                     /locus_tag="VHJ57_00225"
     CDS             complement(1908..2483)
                     /locus_tag="VHJ57_00225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2486992.1"
                     /translation="MKIYTKTGDKGLTGLIDGKRIPKSDIRIIAYGSIDELNSFIGLS
                     ISLLSQNKTNNNNNNQSFSDIIITLNRIQNELFIIGSDLADPDLSKSTSLRVQSTMIT
                     VLENDIDNYEKELSPITYFILPGGSVESSTLHIARSITRRAETNVSKLLLKDTINNLV
                     LIYLNRLSDLLFVLSRTVNKRLQILDIAWKS"
     gene            complement(2542..2712)
                     /locus_tag="VHJ57_00230"
     CDS             complement(2542..2712)
                     /locus_tag="VHJ57_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486993.1"
                     /translation="MNNSPNPEPNQVIETAEERKQRFLLEFDQPFKCDKCNERFKKKK
                     YLREHKAEVHSY"
     gene            2888..2993
                     /locus_tag="VHJ57_00235"
     tRNA            join(2888..2918,2951..2993)
                     /locus_tag="VHJ57_00235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2954..2956,aa:Phe,seq:gaa)
     gene            3035..3199
                     /locus_tag="VHJ57_00240"
     CDS             3035..3199
                     /locus_tag="VHJ57_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486994.1"
                     /translation="MESIIQILRRIFCSHDDSFSFRVYNDFDFSEYNKCTKCGKTLGY
                     FKGFDEDLRG"
     gene            3209..3412
                     /locus_tag="VHJ57_00245"
     CDS             3209..3412
                     /locus_tag="VHJ57_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486995.1"
                     /translation="MLRINLQILIISWWLGAATALLSFLLPIYTHHIIVGGIGWSIIF
                     ISTILIIYEIKKIKKEDKRTKEL"
     gene            complement(3420..3674)
                     /locus_tag="VHJ57_00250"
     CDS             complement(3420..3674)
                     /locus_tag="VHJ57_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2486996.1"
                     /translation="MPTAYILVNCTLGSEEKIISELTNLPDVKEVRGTYGVHDVFVKV
                     RSNDTESMNNTITNKIRKVPGITSTVTLVVIEEQGGKESL"
     gene            3829..>4667
                     /locus_tag="VHJ57_00255"
     CDS             3829..>4667
                     /locus_tag="VHJ57_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2486997.1"
                     /translation="MRLPLKDSDYFVIRVNETIKTIDEEFFNFLKPKLQMKKVDVMLG
                     DGTITNAQTFDLTEITNEFRRMLQSLKDWAFTDISDSKTEDLYRLYFQITCNVDKFYI
                     YGYFGIQFHALHYYKLDKEVINIQKELIKLNHDVSDSFISLANKGNAIIEEELRRQGL
                     DNLSFEELFTKIYENSNLMNNLEHKVLKLEKRFPVIQDSENKREQLISKLNDMIIKLY
                     HIISVKIDYNRLMQGEEGVISYFEIETIKNKKTGKREPYLDTKKFNKEITEIVTSKLN
                     EIAS"
BASE COUNT         1638 a          650 c          654 g         1725 t
ORIGIN      
        1 cccacaacta ttattttccc tttttgtctt gctgctactt cataaatatc atacgcaact
       61 tttttaatga tatgttgagt aggtctttcg cttgaagtag tgctgctcat gaattgaaat
      121 atgtcaactc cctgacgagg acgttcatca ggtataatgc gtatacctct ctctttgatt
      181 acaatcatat catctttttt gatatctcta atcattttac actccgctat tttattatat
      241 gtatcaacaa ctatacactt atccatcata atatttttga cattcatcca aataccatca
      301 taaaaaatct gtgttggttt attggttgta ctataaaata agtcaggcat taccatgtcc
      361 tttggggcag gttctaacaa tacactttgt gtagtaattg attgagctcc agctctgtaa
      421 atagattcta atatttgatt aagatgattt ttatctttac cttctacaat aagtctagcg
      481 taactaggat cagttttatt cttaccaaca gtgaattcca gtactctaaa ttctcctttt
      541 aaatccatta tattgtcaaa gatattagtt aaaatcattg agtctataag atgtcctttg
      601 acttcaattt cctgcttaaa attattaata ttattattat tattattatt atttaatgac
      661 gccaaattgt aatacaaata gtatatttta agtaattaag tattaatcaa attggaagag
      721 aaagtttccc ggtatattcg gatagactat ggtctttctt gatccgtgat acaatattat
      781 ttttatcttc ttctgaaaac cattgtgcta tagttttagc tattccattt ttatcgcaaa
      841 ttacaataac ataaccactg gcttctttga atacagcaat taagttttct tctccaacag
      901 gaacccatac atttgttttt gtatccttca gagctagagc aggcattgct gcatttccag
      961 tatataaatt taacaaatct tgcgattgtt taacacgttt ctctcctaat ttgagggccg
     1021 tgaaatattg cataacaaca gtatattatg aattcttatc taaatgtttt agtatatttg
     1081 caatggtagg atgaaaatct gatccttcaa tattaccact gaccttcaaa aactgagata
     1141 aaaattcaaa tgaataacca tttctaaaat attgattagc ttgtaagaac atttctatct
     1201 tatctagctg ctttacaaga tgtacttctt ttgtttgatt tagtgtatat tctttccata
     1261 actctgagta aagtattgct attttacttg gaaaagaaga tattattact ttcattgcat
     1321 tatcttcttt ggtatgtttt tctaaaacag aaatttcttc gggcatataa tctcctatta
     1381 tggattctgc tagatcatgt attatacata gcttcattac tttttccgta tcaagaccta
     1441 ataaatcaga aaaaaccatt gacaaagcag ttaatgaata gctgtggtct gcaaccgatt
     1501 cagcttttag aattttggct ttggatatcc atcctgtcct tttaatagtt tttaacttaa
     1561 gaatatagtc tataagatta ctaaatttat aatcattcat tttacattca tataattata
     1621 tatcgattaa attaaaatta ttattcatat cttttattat tattagtatt attatagtat
     1681 aataataaaa attagaggta agacaaaaac aagattatag aaaagatttc taaatttagt
     1741 aaatggaaat atgccatgat aataaatgga tttagataca aatcaaatag atatgctaag
     1801 cctgatgtaa caatagctaa cattatagtt ataatgatta ttattatgac attttgtatt
     1861 tgtcccattt aaagacacta ctttcccttt tttattcaat gaattattta agatttccat
     1921 gctatatcca atatttgaag tcttttattt actgttctac ttagtacaaa aagcagatcc
     1981 gaaagtctat tcaaatatat gagaaccaga ttgttaatag tatctttcaa caataatttt
     2041 gaaacattgg tttctgctct tcgtgtgatg cttctagcaa tatggagagt agaggattca
     2101 acagaaccac ctggtagaat aaaataagta ataggagata attctttttc atagttatcg
     2161 atatcatttt ccaatactgt aatcatagta gattgaactc ttaaagaagt ggactttgaa
     2221 aggtctggat cagctaaatc agagcctata atgaataact cattttgaat cctattaagc
     2281 gtaattataa tatcggaaaa agattgatta ttgttattat tattagtttt attctgtgac
     2341 aaaagagata ttgataaacc gatgaatgaa ttcagttcat ctattgaacc ataggcaatt
     2401 attctaatat cactctttgg aattcttttt ccatcaataa gtcctgttaa acccttgtct
     2461 ccggttttag tatatatttt catataacta attctggatt cagtgaaata atatcctata
     2521 ctttaaccaa ctaactaata attagtaaga atgtacttca gctttatgct ctcttagata
     2581 cttcttcttc ttgaaccttt cattgcactt gtcacattta aaaggttgat caaattctaa
     2641 gagaaatcgt tgtttcctct cttcagcagt ttcgatgacc tgattgggtt caggattagg
     2701 ggaattattc attatgattt cttctaggaa attattttta atctttattt agttttagaa
     2761 aaatagttat gttagattga ttatgtaaaa aattacatat tttttttatg aatatttttt
     2821 attaattagc aagagcaata cctacaaaat agaatatata ttgagtcaat tttaatgaat
     2881 attcaatgcc gtcgtagctc agcctgggag agcactcgac ttgattgatt atctattatt
     2941 caagggtgaa gctgaagacc gagctgtcgt gagctcaaat ctcaccgacg gcattaattt
     3001 atattatatc agagaattta gaaatattgt attattggaa agtataatac aaatattaag
     3061 aagaatattt tgttcacatg acgattcctt ttcctttaga gtgtataatg attttgattt
     3121 tagcgaatat aacaaatgca caaaatgtgg taaaacactt ggctatttca agggatttga
     3181 tgaggatttg agaggatgat ttgaattggt gctaaggatc aatcttcaaa tcttaataat
     3241 tagttggtgg ttgggtgcag ctacagcatt gctctcattt ttacttccta tatatactca
     3301 tcatattata gttggaggaa ttggatggtc aataattttt ataagtacta tacttataat
     3361 ctatgaaata aagaagataa aaaaagaaga taaaagaact aaagaactat aaaacaaaat
     3421 taaaggcttt cttttccgcc ttgttcctct attactacaa gagtaacagt agatgtaata
     3481 ccaggcactt ttcttatttt atttgttata gtattattca tagattctgt atcattagat
     3541 ctaaccttta cgaaaacatc atgaactcca taagttcctc ttacctcttt gacatcgggc
     3601 aaatttgtta attcactgat aattttttcc tcagagccta atgtacaatt aactaaaata
     3661 tatgctgtgg gcatagctta atttagtttt tattaatatt ttagttttgt tttttttagc
     3721 aatttataat agatagaata ataaattttt agttaaatta ccaataaaat ttataattat
     3781 tacacaatat tacaaatcta tatgtattga aaatgattat ttgatgacat gaggttacct
     3841 ctgaaagata gcgattattt tgttataaga gttaatgaaa ctattaaaac catcgatgaa
     3901 gaattcttta attttttaaa accgaaattg caaatgaaaa aagttgatgt gatgttagga
     3961 gatgggacaa taacaaatgc acaaactttt gatctcacag aaattacaaa tgaattccga
     4021 cgaatgttac aaagtttaaa agattgggca tttacggata tttcggattc taaaaccgaa
     4081 gatttgtata gattgtattt tcaaataaca tgtaatgttg ataaatttta catatatgga
     4141 tattttggaa tacaatttca tgctttacat tattacaaat tagataaaga agttataaac
     4201 attcagaaag aattgataaa attaaatcac gatgtatcag attcttttat ttcacttgcg
     4261 aacaaaggaa atgccataat agaagaggaa ttacgaaggc agggattaga taacctatca
     4321 tttgaagaat tatttactaa aatatatgaa aatagtaatt taatgaacaa tcttgaacat
     4381 aaagttttaa aactcgaaaa acgatttcct gtaattcaag atagtgaaaa taaaagagaa
     4441 caattaattt caaaattaaa tgatatgatc ataaaattat atcacataat atctgttaaa
     4501 atcgactaca ataggctaat gcaaggagag gaaggagtta tatcatattt tgaaattgaa
     4561 actattaaga ataaaaaaac cggaaaacga gagccatatc ttgatactaa aaaatttaac
     4621 aaagagataa ctgaaattgt aactagtaaa ttaaatgaaa tagcatc
//
LOCUS       DATAFW010000015         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1766203, whole genome shotgun sequence.
ACCESSION   DATAFW010000015 DATAFW010000000
VERSION     DATAFW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766203"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /gene="rpsI"
                     /locus_tag="VHJ57_00260"
     CDS             <1..84
                     /gene="rpsI"
                     /locus_tag="VHJ57_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2486998.1"
                     /translation="LAGDPRQTESKKFGGPGARRKKQKSYR"
     gene            170..859
                     /locus_tag="VHJ57_00265"
     CDS             170..859
                     /locus_tag="VHJ57_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HEX2486999.1"
                     /translation="MTDNKFSIVRLILNADKFEILVKPDPALDYKLGKKVDISNILVS
                     DEIYSDANKGTRVPNEKLIKNFKTKDQIEIAKQILEHGDVNLTTDQRRRMVEEKKKQI
                     IQYISRNFIDPKTHLPHPPLRIENAMEQIRLVIDPFKRSEEQAKKIIDSLRKILPLKS
                     ENLQLIVTVPSQFSAQSYSIIKGIGDLKDEKWLQDGSLQVIIEINAGIRGTFIERINS
                     ITKGSAQIQEQ"
     gene            993..>1642
                     /gene="rrp4"
                     /locus_tag="VHJ57_00270"
     CDS             993..>1642
                     /gene="rrp4"
                     /locus_tag="VHJ57_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Rrp4"
                     /protein_id="HEX2487000.1"
                     /translation="MLEIKKRYVIPGDVIIEGNYRPLMNVIKKDNVIIATRIGIAESS
                     KDGIKVIPLSGVYIPRINDIVIGKIIDNSSLSWEVDINACFSAHLPAQDVFGRDFSPA
                     KDDMKKRLAPGDLVTARIITFDRTRDPMLTIQEKDLGKISNGEFLKISSTRVPRLIGK
                     RGSMIQTIEQATQTRILIGQNGILVVTGKNDEGIALAFKAIKLVEEEAHTSNLTQKV"
BASE COUNT          655 a          210 c          272 g          505 t
ORIGIN      
        1 ctcgcaggag atcccagaca aactgaatca aagaaattcg gtgggccagg tgctcgaaga
       61 aaaaaacaga aatcataccg ttaattatta tttttattta cattgaaata tttcaattag
      121 tttaaatctc tatgcatata tattacaaaa tctcattttt aataattata tgactgataa
      181 taaattctca atagtccggt taatacttaa tgcagataaa ttcgaaattt tagttaaacc
      241 tgacccagca ctagattaca agctaggaaa aaaggtagat atttctaata ttttggtttc
      301 agatgaaatt tattctgatg ctaataaagg tacacgtgtt cctaatgaaa aattaattaa
      361 aaattttaaa actaaagatc aaatagaaat tgcaaaacaa atacttgaac atggtgatgt
      421 taatcttaca actgatcagc gtaggcgaat ggttgaagag aagaaaaaac agattattca
      481 atatattagt agaaatttta ttgatccaaa aacgcatctt ccccatccgc cattaagaat
      541 agaaaatgca atggaacaaa ttcgtttagt aatagatccc tttaagagat cggaggaaca
      601 agcaaaaaaa attattgatt ctcttagaaa aattttacca ttaaaatcag aaaatcttca
      661 attaattgtt actgttccat cacaattttc tgctcagtct tatagtatta taaaaggcat
      721 aggagatctc aaagatgaaa aatggcttca ggatggatct ttacaagtaa ttatagaaat
      781 aaatgctgga atacgcggaa ctttcattga aagaataaat tctattacaa aaggctctgc
      841 tcaaattcaa gaacaataat aacaatgaat aaataccatt tattaaaata ggaaattgga
      901 acttgagaaa agagataaaa ttgttatata gtgataaaaa caagatactt ttctttatta
      961 atgttactaa taaggagatg ttattataat tcatgttaga aataaagaag agatatgtta
     1021 ttccaggtga tgtgattata gaaggaaatt atagaccatt aatgaatgta ataaaaaaag
     1081 ataatgttat aattgctact agaataggga ttgccgaatc aagtaaagat ggcataaaag
     1141 ttattccctt atcaggtgtt tatattccga gaataaatga tatagtcatt ggaaaaatta
     1201 tagataattc ttcattatct tgggaagtag atattaatgc atgtttttca gctcatcttc
     1261 cggctcagga tgtgtttggg agagattttt ctcctgcaaa agatgatatg aaaaaaagac
     1321 ttgcgccagg tgatttggta acagcgagaa taataacttt tgacagaaca agagatccaa
     1381 tgttaaccat tcaggaaaag gatttaggta agatttctaa tggtgaattt ttaaaaatat
     1441 cctcaacacg tgttcctcgt ttaataggaa aaagaggttc gatgatacaa acaattgaac
     1501 aagctacaca aactagaatt ttaattgggc aaaatgggat acttgttgta actggaaaaa
     1561 atgatgaagg tattgctctt gcttttaaag ctataaaatt agttgaggaa gaagcacata
     1621 cctcaaattt aacacaaaaa gt
//
LOCUS       DATAFW010000016         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1766210, whole genome shotgun sequence.
ACCESSION   DATAFW010000016 DATAFW010000000
VERSION     DATAFW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766210"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..411
                     /locus_tag="VHJ57_00275"
     CDS             37..411
                     /locus_tag="VHJ57_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HEX2487001.1"
                     /translation="MEDDEVKTMFAHTLNDNIKWTLRIAYKKTKTPLWKALEKKIESS
                     RSNKSEINIGKLENLTKDGETIIIPGKVLGNGVLTHKLNVAAFSISIPAINKILDAGG
                     KVITINDFVNQYSDGKGVRIIG"
     gene            404..886
                     /locus_tag="VHJ57_00280"
     CDS             404..886
                     /locus_tag="VHJ57_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX2487002.1"
                     /translation="MDNKSNNNNTTVSNINKQIIVDASNCIAGRICSNVSKLLIQGNR
                     VAIINSERAMLSGNRYKTIEDYKKHLEVGSVTNPIHGPYHPRRPDRILTKMVRGMISR
                     RKSSGILAFKRLRVYIGMPTELQSSKEIKIFDDAKITKSESYYITIGDIAKQIGWKGE
                     "
     gene            893..1345
                     /gene="rpsI"
                     /locus_tag="VHJ57_00285"
     CDS             893..1345
                     /gene="rpsI"
                     /locus_tag="VHJ57_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX2487003.1"
                     /translation="MTKTTTIDLYPGQRKTSRAVATISKGNGKVRINNIPAEIIQPDI
                     SKELILTPMNIIGDIRDKIDITVKVEGGGFMGQAFASAVAISRALSGEAKGGRDPKDH
                     PLSKSIRQEIRKKITEFDRHLLAGDPRQTESKKFGGPGARRKKQKSYR"
     gene            1431..2120
                     /locus_tag="VHJ57_00290"
     CDS             1431..2120
                     /locus_tag="VHJ57_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HEX2487004.1"
                     /translation="MTDNKFSIVRLILNADKFEILVKPDPALDYKLGKKVDISNILVS
                     DEIYSDANKGTRVPNEKLIKNFKTTDPIEIAKQILEHGDVNLTTDQRRRMVEEKKKQI
                     IQYISRNFIDPKTHFPHPPLRIENAMEQIRLVIDPFKRPEEQAKKIIESLRKILPLKS
                     ENLHLIVTVPSQFSAQSYSIIKSIGDLKDEKWLQDGSLQVIIEINAGIRGTFIERINS
                     ITKGSAQIQEQ"
BASE COUNT          928 a          296 c          363 g          669 t
ORIGIN      
        1 tctaaaacta ggtagattta tttggggatt attaatatgg aagatgatga ggttaaaacc
       61 atgtttgctc atactttaaa tgataatatt aaatggactt tgcgtattgc atacaaaaaa
      121 actaaaactc ctttatggaa agcactagaa aaaaagatcg aaagttctcg atctaataaa
      181 tctgaaatta atattggtaa attagaaaac cttactaaag atggagaaac tattataatt
      241 ccaggaaagg tattaggtaa tggggttctt acacataaat tgaatgtagc tgcattctcc
      301 atttcaattc ctgcaattaa taagatactt gatgcaggag gaaaagttat taccatcaat
      361 gattttgtaa atcaatattc agatggaaaa ggtgtaagaa taattggata ataaatctaa
      421 caataataat actactgttt caaatatcaa taaacaaata atagttgatg cttccaattg
      481 tattgccggt cgaatatgtt ctaatgtatc gaaactttta attcaaggca atagagtagc
      541 aataatcaat tctgaaagag caatgttatc aggaaataga tataaaacaa ttgaagatta
      601 taaaaaacat cttgaagtag gttctgttac taatcccatc catggacctt atcatccaag
      661 aagaccagat agaatattga ctaaaatggt tagaggaatg atttcaagga gaaagtcaag
      721 cggtatccta gcctttaaac gtttgagagt ttatatagga atgccaacag aattacaaag
      781 ttcaaaggaa ataaaaattt ttgatgatgc taaaataaca aaatctgaat catattacat
      841 tacaattgga gatattgcaa aacaaatagg ttggaaagga gaataataag agatgactaa
      901 aactactact atagatctat atccaggtca gagaaaaacg tcaagagccg ttgctactat
      961 ttccaaagga aatggtaaag ttcgaattaa taatattcca gcagaaatca tacaaccaga
     1021 tatctcaaaa gagttgatat tgactcctat gaatataatt ggagatataa gagataaaat
     1081 tgatattacc gtaaaagttg aaggtggtgg atttatggga caagcttttg caagtgcagt
     1141 agcaatatca cgagccttat caggagaagc taaaggagga cgtgatccaa aggatcatcc
     1201 attatcaaaa agtataagac aagaaatccg aaaaaaaata actgaatttg atagacattt
     1261 actcgcagga gatcccagac aaactgaatc aaagaaattc ggtgggccag gtgctcgaag
     1321 aaaaaaacag aaatcatacc gttaattatt atttttattt acattaaaat atttcaatta
     1381 gtttaaatct ctatgcatat atattacaaa atctcatttt taataattat atgactgata
     1441 ataaattctc aatagttcgg ttaatactta atgcagataa atttgaaatt ttagttaaac
     1501 ctgacccagc actagattat aagctaggaa aaaaagtaga tatttctaat attttagttt
     1561 cagatgaaat ttattcagat gctaataaag gtacacgtgt tcctaatgag aaattaatta
     1621 aaaattttaa aactacagat ccaatagaaa ttgcaaaaca aatacttgaa catggtgatg
     1681 ttaatcttac aactgatcag cgtaggcgaa tggttgaaga aaagaaaaaa cagattattc
     1741 aatacattag tagaaatttt attgatccaa aaacacactt tcctcaccca ccattaagaa
     1801 tagaaaatgc gatggaacaa attcgtttag taatagatcc cttcaagaga ccagaggaac
     1861 aagcaaaaaa aattattgag tctcttagaa aaattttacc attaaaatca gaaaatcttc
     1921 atttaattgt tactgtccca tcacaatttt ctgctcaatc ttatagtatt ataaaaagca
     1981 taggagatct caaagatgag aaatggctgc aagatggatc tttacaagta attatagaaa
     2041 taaatgctgg aatacgtgga actttcattg aaagaataaa ttctattaca aaaggatctg
     2101 ctcaaattca agaacaataa taacaatgaa taaataccat ttattaaaat aggaaattgg
     2161 aacttgagaa aagatataaa attgttatat agtgatacaa ataagatact tttctttgtt
     2221 aatgttacta ataaggagat gttattataa ttcatg
//
LOCUS       DATAFW010000017         8221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_709171, whole genome shotgun sequence.
ACCESSION   DATAFW010000017 DATAFW010000000
VERSION     DATAFW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709171"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="VHJ57_00295"
     CDS             <1..1823
                     /locus_tag="VHJ57_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2487005.1"
                     /translation="KDYKVKGVLLEKMVPKGIELIVGLQNDPQFGPVMMLGLGGIYTE
                     LFKDISFRVLPITKEDVLDMIDDLKAKVILKGFRGAEPINLEMLCDAILKIGNLGMDI
                     AKYIDSIDFNPIIVYPNNYYVVDAKILLKSVSDENIISQSIPDSSYMNTFFNTNSIAL
                     IGASPEEGKIGNSVLKSLSKYDYKGKIYPVNAKGYNEILGLKAYKNLGEISENIELVI
                     VTVDLKFVPAILETCGTKGIHNMVIISGGGKELGGERAEIEQKVKELSSQLKVRIIGP
                     NCIGMFNGENRLDCAFQGHDRMIRPKKGDVAFLSQSGTIGIAFMETSDSFGMSKMISY
                     GNRSDVDEADMIWYLSEDPKTNVIGLYVEGLGDGRKFMNTAKRVIKEKKKPIVVFKNG
                     RTQRGAKQAASHTGSLGGSYSVVKGAFDQAGIISVDSYEELTGSLKALTWQPTPHGNN
                     VALLTNGAGPIIAAIDHFERLELKIAELTDETKQKFIQHYPPTYVIGNPLDVTGSANA
                     DDYKFAIQTYMDDPTIDIIMPWFVFQDDPLEESIVNILADFQKQKKKPILIGALGGPF
                     TIDISKRLEENNVPVYHSVISWVTAAGVLAKWAKLQNIKS"
     gene            1793..2227
                     /gene="erpA"
                     /locus_tag="VHJ57_00300"
     CDS             1793..2227
                     /gene="erpA"
                     /locus_tag="VHJ57_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX2487006.1"
                     /translation="MGKASKYQVLVSLQRRGKLIFFLKPNYNMESTQQSQIVTVTPKA
                     AEKVSEFMKQEGKDSLFLRVYVTGGGCSGLSYGMGFEENSEEDDLVIEQNGVKILVDN
                     YSQRYLKGANIDYIDSLMGSGFKINNPNVTKSCSCGHSFSTE"
     gene            complement(2304..3572)
                     /locus_tag="VHJ57_00305"
     CDS             complement(2304..3572)
                     /locus_tag="VHJ57_00305"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX2487007.1"
                     /translation="MSSSNLNEKINPFEVALKQLEEASKILNLDKGMLEILSNPKRIL
                     TVSIPTRMDDGSIKVFTGFRSQHNDARGPHKGGIRYHPDVTLDEVKALSMWMTWKCAI
                     ANIPYGGGKGGIICNPKQMSENELERMTRRFAYLIADIIGPYRDIPAPDVYTGGKEMA
                     WIMDTYSALKGNYVQPEVITGKAIAIGGSLGRTEATGRGLAFTVREAAKKRGIDMKQA
                     TFVVQGFGNAGQFSSKLVEEQGAKMIAASDSEGAIINTNGISADALMKFKSETGSVVG
                     FGNAKSISNDELLETECTILIPAALENQITKNNADKIKTQIIAEAANGPTTPEADEIL
                     YKNNILVIPDVLANGGGVTVSYFEWLQNLRREYWTEEEVNNRLDRNITKSFFDVYDAH
                     EKYKVHMRKAATLLAVERVVEAINIRGLWP"
     gene            complement(3620..3976)
                     /locus_tag="VHJ57_00310"
     CDS             complement(3620..3976)
                     /locus_tag="VHJ57_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2487008.1"
                     /translation="MGIGGSEWVIIGLITIFLLFGSRKLPDIARHIGKAIGEYNNTKQ
                     TLLGEINKVKFNNNSFDHQPNEKNLYLGKIIDGPVSTEREKLERIAMSLNIEYLDMTD
                     DQLRLIILQKMKNDNL"
     gene            4175..5446
                     /gene="dnaG"
                     /locus_tag="VHJ57_00315"
     CDS             4175..5446
                     /gene="dnaG"
                     /locus_tag="VHJ57_00315"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF003108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HEX2487009.1"
                     /translation="MELPNTGIVKYHVKLSFDVDGLVEKADIIGAIFGQTEGLLGPEM
                     NLNELQKVSKVGRIEVNVDTKSNIAKGDALIPMSTDISTAALIAAAIESIDKVGPFQA
                     RFELAGIDDIRAIKKKVIVDRAKKIVQEWATKTISEGEEMLKDVYDASKPGKLTTFGK
                     GQLACGTGVLNADWIILVEGRADVINLLRAGFDNSIAIEGAKIDESIIHLTEGKKVIA
                     FLDGDRAGDLILKELHGLVKIDKVLRAPPGREVEECTPLEITEILNNAVDYIKEESLS
                     SIKKQQTVLQNHKKYNKNDREKTSYPQTQPQFNQLETYPITKEENGLQTNNESKSVLR
                     NIYSQINETLEAILLDGSMNTVMKVPVSEIVKKLSDSSGGKILVLDGIVTQRLIDASD
                     KAGIEQIIGHRLADIKRTPSIKVGTFSELGI"
     gene            5458..6153
                     /locus_tag="VHJ57_00320"
     CDS             5458..6153
                     /locus_tag="VHJ57_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HEX2487010.1"
                     /translation="MSNITFQHVVILSELLLCGAKNNFVELTTNEIAKKIKRSQQLAS
                     KELLDLEFFGCIKRNKKSKKYTIKVTEKGYQQVYDLFLFLKTAVESFKHVIDFEGHII
                     TGMGEGAYYMSLNGYKKQFKEKLGYEPYPGTLNIKLNSMSFIDAKKEMLKYPSIYIDG
                     FSDQSRTFGWVKCYPAYINDSININASILILERTHYDDSIIEIIAPFSIKEQFSLKNG
                     DYIKLKVNTNNPI"
     gene            complement(6164..6772)
                     /locus_tag="VHJ57_00325"
     CDS             complement(6164..6772)
                     /locus_tag="VHJ57_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX2487011.1"
                     /translation="MKAIYISNLDPSKSFLRYLQEKYQLDTSFFESRIRGLVDLGLIG
                     KINHERYKLNSLGRETMKVVLVGGVFDILHPGHISTLKAAKLHGDILVVVIATETTAT
                     KIKKNRVIFHNENLRREMVSSLNFVDMALIGKEGTLFDTVEHVKPDIIALGYDQSHTE
                     KFIAENCKKRNINVRILRLNTPIPNMKSSELKRDLGDSFYNI"
     gene            7029..7205
                     /locus_tag="VHJ57_00330"
     CDS             7029..7205
                     /locus_tag="VHJ57_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487012.1"
                     /translation="MDSSTRNFICKSCGLYASRDKIEELKDKSQISSSDSKQRTHDDY
                     LDWWQSSKKEKQRN"
     gene            complement(7216..7716)
                     /locus_tag="VHJ57_00335"
     CDS             complement(7216..7716)
                     /locus_tag="VHJ57_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2487013.1"
                     /translation="MNIRPNLLDYINCRILDILSKNSSLPFVELAKQIGISDATIHTR
                     VKKLLTLGVIKKFTIFVDNDLLGYDHLAFINLKCERGRTEETTKNLMDIDEILEIHEI
                     YDKFDLLIKIRSKNLDNLRDIIVNKILSINYVKETELMTVLRTRKEEQMISLQKEVSE
                     KTEEFF"
     gene            7898..>8221
                     /locus_tag="VHJ57_00340"
     CDS             7898..>8221
                     /locus_tag="VHJ57_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2487014.1"
                     /translation="MQRSLCSKCQKIECVYFRQYSGEYLCRSCFIISIEEKTYKTISK
                     FSMINYGDKIAIGVSGGKDSLSLLLVLNRIFKRKKTNDIIAITIDEGIAGYRNESLRI
                     VKDFCS"
BASE COUNT         2871 a         1238 c         1299 g         2813 t
ORIGIN      
        1 ctaaagacta taaagttaaa ggagtcctct tagaaaagat ggttcctaaa ggaattgaat
       61 taatagtggg tttgcaaaat gatcctcaat ttggtccagt catgatgctt ggcttaggag
      121 gaatttacac cgaattattc aaagatatat cattcagagt ccttccaata actaaggaag
      181 atgtattaga tatgatagac gatctaaaag ctaaagtgat cttaaaaggg ttcagaggag
      241 cagagccaat aaatcttgaa atgctttgtg atgcgattct caaaataggt aatttgggaa
      301 tggatatagc taaatatatt gatagcatag attttaatcc cattatagtt tatccaaata
      361 attattatgt cgttgatgca aaaattcttc taaaatccgt ttccgacgaa aatatcatct
      421 ctcaatcaat acctgattcc agttatatga atacattctt taatacgaat tcaatagcat
      481 taataggagc atctcccgaa gaaggtaaga taggcaattc tgtattgaaa agtttatcta
      541 aatatgatta taaaggtaaa atatatccag taaatgcaaa gggttataat gaaattttag
      601 gattaaaggc atataaaaat ttaggagaga ttagtgaaaa catagagcta gttatagtca
      661 cagttgattt aaaatttgtt ccagctatat tggaaacatg tggaacgaaa ggaatacata
      721 atatggtaat catttctgga ggaggaaaag aactcggtgg agaaagagca gaaatagaac
      781 aaaaagtgaa agaattatct tctcaattaa aagttagaat aatcggtcca aattgtatag
      841 gaatgtttaa tggtgaaaat cgattagatt gtgcttttca aggacacgat agaatgatta
      901 gaccaaaaaa gggagatgtt gctttcttat ctcaaagtgg aactattgga attgctttta
      961 tggaaacttc agactctttt ggaatgagca aaatgatttc ttatggtaat cgctcagatg
     1021 tagatgaggc agatatgatt tggtatttat cagaagatcc aaaaacaaat gtaattggtc
     1081 tctatgttga aggtcttggt gatggtagaa agtttatgaa tactgccaag agagttataa
     1141 aagaaaaaaa gaaacctata gtagttttta aaaatgggcg aacacaaaga ggagctaaac
     1201 aagcagcatc tcatacagga tctttgggag gatcatattc tgtagtcaaa ggagcatttg
     1261 atcaagctgg tattatatct gttgatagct atgaggaatt aacgggatca ttaaaagctt
     1321 taacttggca acctacccca cacggtaata atgttgcttt acttaccaat ggggcaggac
     1381 caataattgc tgcaatagat cattttgaaa gactagaatt aaaaattgct gagttaacag
     1441 atgaaactaa acaaaagttt attcaacatt atccaccaac atacgttata ggcaatcctc
     1501 ttgatgtaac gggttctgca aatgctgatg attacaaatt cgccattcaa acatatatgg
     1561 atgatcctac tattgatatc ataatgccct ggtttgtttt tcaagatgat ccgcttgaag
     1621 aaagtattgt aaatatatta gcagattttc aaaaacaaaa gaaaaaacca attttgatag
     1681 gcgcattagg tggaccattt accattgata tctcaaaaag attagaagaa aataatgtac
     1741 cagtgtatca ttcagtaata tcatgggtca cagcagctgg agtacttgca aaatgggcaa
     1801 agcttcaaaa tatcaagtcc tagtgtcttt gcaaagacgg ggaaaactta tattcttttt
     1861 aaaacctaat tacaatatgg aaagtacaca acaatcgcaa atagtaactg ttactccaaa
     1921 agccgcagaa aaagttagtg aatttatgaa acaagaagga aaagatagtt tattcttgcg
     1981 ggtatatgtc actggtggag gctgttctgg cttatcttat ggtatgggtt tcgaagaaaa
     2041 ttctgaagaa gatgatcttg ttatagagca aaacggggtc aaaattttgg tagataatta
     2101 tagtcaaaga tatttaaaag gtgctaatat tgattatatt gatagcctta tgggatctgg
     2161 attcaaaatt aataatccaa atgtaaccaa aagctgttca tgtggtcatt cattttctac
     2221 tgagtaaatc actttttcaa ttattttata atttattgat gctaaaattt tgagtctagt
     2281 agaaattaaa aataaaaatg ttattatggc cataaccctc tgatattaat agcttcaaca
     2341 actctctcta ctgctagtaa ggtcgcagcc tttcgcatat gtaccttgta tttttcatga
     2401 gcatcataaa catcaaagaa tgatttcgtg atgtttctat ctaatctatt attaacttct
     2461 tcttcggtcc agtattctct tcttaaattc tgtaaccatt caaaataaga aacagttaca
     2521 cctcctccat ttgcaagcac atcaggaatc accaagatat tatttttata caatatttca
     2581 tctgcttcag gagttgtagg cccgttagct gcttctgcta tgatttgtgt ttttatttta
     2641 tctgcattat ttttagtaat ctgattttcc aacgctgctg gaatgagaat agtacattct
     2701 gtttccaaaa gttcatcatt agaaattgat ttcgcattcc caaaacctac tacgctccct
     2761 gtttcacttt taaatttcat taatgcatca gcagagatcc catttgtatt aattatagca
     2821 ccttcagaat ctgaggctgc tatcatctta gcaccttgct cttctactag tttggaggaa
     2881 aactgtcctg cattcccaaa accttgaact acaaatgtag cttgtttcat atcaatacct
     2941 ctttttttag cagcttctct gacggtaaat gccaaacctc tacctgtggc ctcagttcta
     3001 cctaatgaac caccaattgc tattgctttt ccagttatta cctctggttg aacataattt
     3061 cctttcaatg cagaataagt atccataatc catgccatct ctttacctcc tgtatataca
     3121 tctggtgcag gaatatccct atatggacct ataatatcag ctataaggta tgcaaacctt
     3181 ctagtcattc tttctaattc attttctgac atttgttttg gattgcaaat tattccacct
     3241 tttccaccgc cataaggaat attcgctatt gcacatttcc atgtcatcca cattgataaa
     3301 gccttaactt cgtccaatgt aacatcagga tgatatctta ttcctccttt gtgaggccct
     3361 ctggcatcat tatgttggga tcgaaacccc gtaaacacct taatcgaacc atcgtccatt
     3421 cttgttggaa tagaaacagt tagaattctt ttgggattgg ataatatctc cagcattcct
     3481 ttatctaggt ttagaatttt tgatgcttct tctagttgtt ttagtgctac ttcaaatgga
     3541 tttatttttt catttaaatt agatgaagac atatatttta ttcataatgt gttgtttata
     3601 ttagataata aatatatctc tacaaattat catttttcat cttttgcaaa ataattaaac
     3661 gcagttggtc atcagtcata tccagatatt ctatattgag tgacattgca attctttcta
     3721 gtttttctct ttctgttgat acaggtccat ctattatctt tcccaaatac aaattttttt
     3781 cgttaggctg atgatcaaat gagttattat tgaatttaac tttatttatt tctccaagaa
     3841 gggtttgttt tgtattatta tactcaccaa ttgctttgcc tatatgtcgt gctatatctg
     3901 gtaattttct tgaaccaaaa agtaaaaaga tagttattaa accaattatt acccattctg
     3961 atccaccaat tcccatataa tgtataagaa ctaatgataa tttatttatt aattatgcat
     4021 ttatcaatta tataggctta tcaagaatat taaaattact tttaaaaaaa gaatcacaaa
     4081 tcttatactt gtcacattta ggtattggta ttggtcaaag cacttatatt cgtgccaatc
     4141 acccataatt ttactttaca ataaacatga ggttatggaa ttgcctaata ctggtatagt
     4201 aaaatatcac gttaaattaa gtttcgacgt agatggactc gtagaaaaag ctgatataat
     4261 tggtgctatt tttggccaaa ctgagggact cctaggaccg gaaatgaatt tgaatgaatt
     4321 acaaaaggtc tccaaagtcg gtcgaatcga agtcaatgtg gatacaaaat cgaatatagc
     4381 aaagggtgat gctcttattc ctatgagcac tgatatctct actgctgctc ttatagctgc
     4441 tgcaatagaa agtatagata aagttggacc atttcaagca agatttgaat tggctggtat
     4501 tgacgatatt cgtgccataa agaaaaaagt tatagtagat agagcgaaaa aaattgtcca
     4561 agaatgggct actaaaacca ttagcgaagg tgaagaaatg ctcaaagatg tatatgatgc
     4621 aagtaaacct ggaaaactta ctacatttgg aaaaggccaa cttgcatgtg gcacaggtgt
     4681 attaaatgct gattggatta ttttagtcga aggtagagct gatgttatta atctcttgag
     4741 agcaggattc gataattcta tagctataga aggtgctaag attgatgaaa gtattatcca
     4801 tctaactgaa ggaaaaaaag ttatagcttt cttagatggt gatagagcgg gtgatttaat
     4861 tttaaaagaa ctccacggct tagttaaaat cgataaagta ttgagagccc ctccaggaag
     4921 agaagttgag gaatgtacac ctttagaaat aaccgaaata ttgaataatg cagtggacta
     4981 catcaaagaa gaatcactct cgtctataaa aaaacaacaa actgtattac aaaatcataa
     5041 aaaatataac aaaaacgata gagaaaaaac ttcttatcca cagacacaac cacagtttaa
     5101 tcaattggaa acatatccaa ttaccaaaga agaaaatgga ttacaaacta ataatgaatc
     5161 caaatcagtt ttaagaaaca tatattccca aatcaatgaa accttggaag caattcttct
     5221 ggatgggtca atgaatactg ttatgaaagt tcccgtttct gagatagtta aaaaactttc
     5281 agattcatca ggcggtaaaa tattggtttt agatggaata gtaactcaaa gattgattga
     5341 tgcttctgat aaggctggca ttgaacaaat cataggccat agattagctg atatcaaacg
     5401 tactccatct attaaagtag gtacattcag tgaattggga atataataat taattttatg
     5461 agtaatatta cctttcaaca tgtagttatt ctctcagaat tgttgttgtg tggagcaaag
     5521 aataactttg ttgagctaac aactaacgag atagcaaaaa aaataaaacg ctcacaacaa
     5581 ttagcttcta aagaattact cgatcttgaa ttttttggat gtataaaaag aaacaaaaaa
     5641 agtaaaaaat atactataaa ggttacagaa aaaggatatc aacaggtata tgatcttttt
     5701 ttatttctaa agactgctgt agagtctttt aaacacgtta ttgattttga gggtcatatt
     5761 ataactggaa tgggagaagg agcatattac atgtcattaa atggatataa aaagcaattt
     5821 aaagaaaaat taggttatga accttatcca ggaacgctaa atataaaatt aaattctatg
     5881 tcctttatag acgcaaaaaa ggagatgtta aaatatccgt cgatatatat tgatggcttt
     5941 agtgatcaat ctagaacttt tggatgggta aaatgttatc ctgcttatat taacgatagt
     6001 ataaatataa acgcctcaat tttgatattg gaaagaactc attatgatga ttctataatt
     6061 gagataatag ctccattttc cattaaagaa caattttcat tgaaaaacgg cgattatatc
     6121 aagttgaaag ttaataccaa taatcctata taatgaatta acatcatata ttgtaaaatg
     6181 aatctcctaa atctcgtttt aattcagagc tcttcatatt tggaatagga gtatttaatc
     6241 ttaaaatcct aacgttgata tttcttttct tgcaattctc tgcaataaat ttctccgtat
     6301 gtgattgatc ataacctaat gctataatgt caggttttac atgctcaact gtatcaaata
     6361 atgttccttc ttttccaatt aatgccatat ctacaaaatt taatgaagaa accatttctc
     6421 ttcttaagtt ttcattatga aatatgactc tatttttttt tattttagta gctgtagttt
     6481 ccgttgcaat tactactact aaaatatcac catgtaattt tgctgccttg agagtagata
     6541 tgtgaccagg atgtagtata tcaaaaacac ctccaacaag gactactttc atcgtctctc
     6601 ttcctaatga atttaatttg tatcgttcat ggtttatttt tcctattaat cctaaatcta
     6661 ctagtcctct aattcttgat tcaaaaaatg atgtatctaa ttgatatttc tcctgtaaat
     6721 atcgtaaaaa tgattttgat ggatctaaat tacttatata tatggctttc aaaataagtt
     6781 tatctagtga tttcaattct atcccgagat ttttaaatat gataattcat aaataatata
     6841 tattcgtttg atgacaaaaa tatttttcct atcatttcat catttatgaa tcttcatttg
     6901 tcttgttatt tattattgaa ctatcaaaat gctttgtgta atatcttctc ataatacttt
     6961 tattaggttt gcatttattt aaaattgact tggtcaaatt ttgtcctgaa tgtggtggag
     7021 atttaaaaat ggattcatct acaagaaatt ttatttgtaa aagttgtggc ctctatgctt
     7081 caagagacaa aattgaggaa ttaaaagaca aatcacaaat tagttcttct gattctaaac
     7141 aaagaactca tgatgattac ttggattggt ggcaatcctc aaaaaaagaa aaacaaagaa
     7201 attagttttt aaatattaaa agaattcttc ggttttttca ctcacttctt tttgtaatga
     7261 gatcatctgc tcttcttttc tagttcttag aactgtcatt aactctgttt cctttacgta
     7321 attaattgac aatattttat ttacaattat atctctaagg ttgtccaaat tttttgatcg
     7381 aattttaatt agcaaatcaa atttgtcata gatctcatgt atttctaaaa tttcatcgat
     7441 gtccattaga ttttttgtcg tttcctctgt tcttcccctt tcacatttta aattaataaa
     7501 tgctaaatgg tcgtacccaa gcaaatcatt atctacaaaa atagtaaatt ttttaataac
     7561 acccaaagtc aatagtttct tgactctcgt atgaatagtt gcatctgaaa taccaatttg
     7621 cttggctaat tctacaaatg gtaatgatga atttttactt agaatatcca aaatcctaca
     7681 gttaatataa tctaataaat ttggtctgat attcataatt cttcagatat attactaaaa
     7741 tatattaata ctgatttaat ccatgtttaa agatattaag aatctgtatg aattaacatt
     7801 ataaaatgaa aaatatacga gtatcaataa acaatagttt tttgcaagtc tagtttaaat
     7861 taaataccta ttaaatacta aaaaatttat catataaatg caacgttcac tatgttcaaa
     7921 atgtcaaaag atagagtgtg tttattttcg acaatattca ggcgaatatc tttgtagatc
     7981 ttgttttata atttcaattg aagaaaaaac ttataaaact atttcaaaat tttctatgat
     8041 caactatgga gataaaattg caataggtgt ttcaggaggg aaagatagtc tttccttact
     8101 attagttttg aatcgcatat ttaaaagaaa gaaaactaat gatattattg caattacaat
     8161 agatgaggga attgcaggat atagaaatga atctttaaga atagttaaag acttttgttc
     8221 t
//
LOCUS       DATAFW010000018         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3528723, whole genome shotgun sequence.
ACCESSION   DATAFW010000018 DATAFW010000000
VERSION     DATAFW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3528723"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..272)
                     /locus_tag="VHJ57_00345"
     CDS             complement(132..272)
                     /locus_tag="VHJ57_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487015.1"
                     /translation="MATEQIVDPFMVMIAISGFFSATILLSSLLTIRKKKIQTDKIEN
                     NI"
     gene            391..984
                     /gene="dps"
                     /locus_tag="VHJ57_00350"
     CDS             391..984
                     /gene="dps"
                     /locus_tag="VHJ57_00350"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HEX2487016.1"
                     /translation="MSNDQNRSVPKIVALEVLEKNGVDVQRLIELIRKGVGAEFTTYY
                     YYTILRMHCTGLEGEGIKEIVEDARIEDRNHFEAMTPRLYELGGSLPRDIREFADQAG
                     CPDAYLPDNWQDINSILKVLLEAEQCAIRSWGEVCDMTAGKDPRTYDIAQKIMQEEVE
                     HEAWFIELLSKRPSGHFRRKFVGQSPHVSEQGSLIHL"
     gene            1083..1226
                     /locus_tag="VHJ57_00355"
     CDS             1083..1226
                     /locus_tag="VHJ57_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487017.1"
                     /translation="MRRRTAEMIMIGSGLAIAASAGAMNITYMIYGGLAILGLGVISI
                     FWR"
     gene            1293..2357
                     /locus_tag="VHJ57_00360"
     CDS             1293..2357
                     /locus_tag="VHJ57_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2487018.1"
                     /translation="MDPVTIFDKIKDAQCNNKSITVLHDFFIDRIIKIENIEEFFVRI
                     KEKTKYGGGSIRGIATTQFEGGNAVNIAYCLGKLGFDVDLFTITDDSSNMNLKYFFGD
                     LPKVKLHLSYGKPGYTTSLEIRNSIDFNDSNIMISDVGGNSDFGPEKLGPHNDKEILN
                     NSDAVILVNWASNWKGNELLKYVFQNSPKALHFVDPADIQTRSNEFCSLLRDPNSMID
                     ILSINENECNSLLFELELKNFSLCNKYSRKNIQLAIKGLAKKLGIFIDLHTRYGCAYS
                     NGDEVYFAYNIKNIVVRNLTGAGDSWDAADIIGHLIELPPQERLFFSNAFASFYITIE
                     NRQLISLEKFFQYYYNLYNK"
BASE COUNT          944 a          322 c          438 g          867 t
ORIGIN      
        1 gcataatcga ctaacaatac agatactttt tttttgaaag cacaatttac cagtatagct
       61 gaagatagac catcaacatc ttctttatga gaaatacata ttgtaatagt cattttagct
      121 ataattaaat atcaaatatt attttcaatt ttatcagttt ggattttttt ctttctaatt
      181 gttaaaagtg atgataataa tatagttgca gaaaaaaatc ctgaaatagc gatcatgacc
      241 ataaaaggat ccacaatttg ttcagttgcc atatagaata tccaaaaatc attatatata
      301 tatattaaga tggcaaatta taattaatcc gattattttc ttacgtcatg atattaaaac
      361 ctttaaatat tattattcaa atcgaataat atgtcaaatg atcaaaacag atctgttcca
      421 aaaattgtag cattggaagt tttggaaaaa aatggtgtag atgtccaaag attaatagaa
      481 ttaatcagaa aaggtgtagg cgcggagttt acaacatatt attattatac tatcttaaga
      541 atgcattgta caggtttaga gggtgaagga attaaggaaa tagtcgagga tgcaagaata
      601 gaagatagaa atcactttga agcaatgact cctaggttat atgaattagg tgggagtctc
      661 ccaagagata ttagagaatt tgctgatcag gctggctgtc cagatgcata tttaccggat
      721 aactggcaag atatcaattc aatattaaag gtattgctag aggcagaaca atgtgcaata
      781 agatcatggg gagaggtatg cgatatgact gcaggtaagg atccacgaac atatgatata
      841 gcacaaaaaa taatgcaaga agaagttgaa cacgaggcat ggtttattga gcttttgtct
      901 aaaagacctt ctggccactt tagaagaaag tttgttggac aatccccaca tgttagtgag
      961 caaggtagtt taatccactt gtaatttctt taatttttta tatttttcat tttttatagc
     1021 atataacctt taccgcagaa ctacaatact tattaggcca cttttttata attattgaga
     1081 gaatgagacg aagaacagca gagatgatta tgataggttc agggttagca atagcagcaa
     1141 gtgcaggagc tatgaatatt acttatatga tttatggagg attagcaatc ttaggtttag
     1201 gtgtgatttc aatattttgg agataatttt cataaaaaaa caataacaac aatgatgtat
     1261 tataaagaaa tgaaataggt gtttctataa atttggatcc cgttacaatt tttgataaaa
     1321 taaaagacgc acaatgcaat aacaaatcaa ttacagtatt gcatgatttt tttattgaca
     1381 gaataatcaa aattgaaaat atagaggagt tttttgtacg tataaaagaa aaaaccaagt
     1441 atggtggtgg aagcatcaga ggaatagcta ctacacaatt tgaaggagga aatgcggtga
     1501 atattgctta ctgtcttgga aaactcggat ttgatgtaga tctatttact ataactgatg
     1561 attcaagtaa catgaattta aaatattttt ttggtgattt acctaaagta aaattgcact
     1621 tatcctatgg aaaaccagga tacacaacgt cattagaaat ccgtaactct atagacttta
     1681 atgattctaa tataatgatt agtgatgtag gagggaattc agattttggc ccagaaaaac
     1741 taggtcctca caatgataaa gaaatattaa ataattctga tgccgttatt cttgtaaatt
     1801 gggcaagcaa ctggaaaggt aatgaacttt taaaatacgt atttcaaaat tcacctaaag
     1861 cattacattt tgttgatcct gcagatattc aaacccggag taatgaattt tgctcattat
     1921 taagagatcc aaatagtatg atagatattc taagtattaa tgagaatgaa tgtaattctt
     1981 tactctttga attagagctc aaaaattttt ccttatgtaa caaatatagc cgaaagaata
     2041 tacaactagc tattaaaggg ttagcaaaaa aattaggtat atttatagac ctgcacacca
     2101 gatatggttg tgcttattct aatggtgatg aggtttattt tgcatataac ataaaaaata
     2161 ttgttgtacg aaatttaact ggtgctgggg attcttggga tgcggctgat attataggac
     2221 atcttattga attacctcca caagagagat tatttttttc taatgctttt gcttcattct
     2281 atataactat agaaaataga caattaattt cattagaaaa attttttcaa tattactata
     2341 atttgtataa taaataagac atcaaaaaaa atacagcata gacaagattt tactacttta
     2401 tattttattt agtaggtcaa tatttaataa aagtagataa aataaaatat attattttgt
     2461 tagtgccgtt tttgttacta attcttcaat ttttttgttt atgtctaaag ttactttaag
     2521 caagtctggt gtccatcctc tttttctgat aaatgtagac aaggctttag t
//
LOCUS       DATAFW010000019         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2119550, whole genome shotgun sequence.
ACCESSION   DATAFW010000019 DATAFW010000000
VERSION     DATAFW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119550"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VHJ57_00365"
     CDS             complement(<1..194)
                     /locus_tag="VHJ57_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487019.1"
                     /translation="MDREEILRSLFTKAIEDTIETLPYTIQVFQDPLSDLKKTVTNDL
                     DFLLGAFFAQVLLFYSVYCS"
     gene            complement(556..2427)
                     /gene="polX"
                     /locus_tag="VHJ57_00370"
     CDS             complement(556..2427)
                     /gene="polX"
                     /locus_tag="VHJ57_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX2487020.1"
                     /translation="MHLQCFVKAFGLSLVVLYRENVTMSTNSEAAKIFREIAFILQIL
                     EDKKSEPNAIFKIRSYNRAADEIQNLSSNIGEIYKEEKLKGLLKIPSIGKAIATKLEE
                     YITTGKIHYYDELKEKLPIDINQFFGLEGIGPKTIKTLYDNLGIKNISDLEKVALEGK
                     IRNIPGFSEKKEELILKKIQFFRKGGGRRLIGEVYPLVRRIEKRLSGNNGVKQAVVVG
                     SFRRMKETIGDIDYLVSVISEKDGNNIIDYFVNMAEVKEVSGRGPSKAFVKLNNGIDA
                     DLLVVPEESFGAAMQYFTGSKEHGIAMRKIAISKGLRLNEWGVFDKQNQRVAGKTEEG
                     VYKILGLEWIPPEMRENRGEIELAKIGKNGMEKEEEKVRLPQLIAYDDLKGDLQVHSN
                     NTDGTMSIEDMALNARDKFGLSYIAISDHTKSLRLTNGLDENQLLNQANTIAELNDKI
                     KMNNNNKHFRILSSAEVNILKDGSLDISNNVLDKLDIVGAAIHSHFSLPIELQTARLV
                     SAAQNPSIDIIFHPTGRIINRREGYPINISKLIEVANESNTILEIDAHYDRLDLKDEF
                     IRMAVENNVKLVIDSDAHHPLHYAFLIFGIGQARRGWAKKGDILNTLSAKGLLDSLK"
BASE COUNT          904 a          530 c          398 g         1130 t
ORIGIN      
        1 tttgaacaat aaacagaata aaacaaaagt acttgtgcaa agaatgctcc taaaagaaaa
       61 tccagatcgt tggttactgt tttctttaaa tcacttaatg ggtcctgaaa tacttgaatt
      121 gtataaggta aagtttctat cgtatcttca atggccttag taaataaact tctaagaatt
      181 tcttctctat ccaacaatat caataatata aacttgttaa ataatttatt ttcttagatc
      241 gtgtactgta ttcaataaaa ttattattta aatttataaa gaaaccattc tataaccttt
      301 caaatattta gtagttatat agagaaatat tttgtttatc tttgttaaat tcctgttgat
      361 tacttttatc atctaaaaat acagaaccaa ttatgaaata ttttaaaacc gaatatacag
      421 gggatgaact atgtactata atttctcatt atacaagttt aatgatagac gattttatgg
      481 tcggtatgaa cgttaaagat tataggagtc tgtttctatt ttgctcacac attaatcatg
      541 aatgctccgg tctgactact ttaggctatc aagtaatcct ttagctgata gagtattaag
      601 gatatcaccc ttcttggccc atcctctcct ggcttgtcct ataccgaaaa ttagaaaagc
      661 ataatgtaga ggatgatgag catcagaatc aattaccaat ttaacgttat tttcaacagc
      721 cattcgtatg aattcatctt ttaaatctaa ccggtcataa tgagcgtcaa tttcaaggat
      781 ggtattggat tcatttgcta cttcaatcaa tttggatatg ttgataggat atccttctct
      841 tctattaata attctgcctg tgggatgaaa tataatatca atactaggat tttgagctgc
      901 acttacgagt ctagctgtct gcaactctat tggtaaagaa aagtgagaat gaatagcagc
      961 acctactatg tctagtttgt ccaagacatt atttgaaata tcaagtgaac catcctttag
     1021 aatatttact tcagcagaag ataatatacg aaaatgcttg ttattattat tcatcttaat
     1081 tttgtcattt agttctgcaa ttgtatttgc ttgatttaac aattggtttt catctagtcc
     1141 attagtaagc ctcaagcttt ttgtatgatc ggagattgcg atataactca atccaaattt
     1201 gtctcttgca tttaatgcca tatcttctat agacattgtg ccatcagtgt tattgctatg
     1261 aacttgtaag tctcctttca aatcatcata tgctattaat tgtggcagcc ttactttctc
     1321 ttcttctttt tccattccat ttttccctat ttttgctagc tctatttctc ctctattttc
     1381 tcgcatctct ggaggaatcc attctaagcc caatatttta tatactcctt cttcagtctt
     1441 gcctgctact ctctgattct gtttgtcaaa aaccccccac tcatttagac gaagaccttt
     1501 agatatcgct atttttctca tagctattcc atgttcttta ctgccggtaa agtattgcat
     1561 cgctgcacca aaactttctt caggtacaac aagtaaatct gcatcaatcc cgttgtttag
     1621 tttgacaaat gctttagatg gtcctcttcc gcttacttct ttaacttctg ccatatttac
     1681 aaaataatct attatattat ttccatcttt ttcagatata actgacacca aataatcaat
     1741 atctcctata gtttccttca ttcgtctaaa agaaccgaca actactgctt gttttactcc
     1801 attatttcct gaaagacgtt tctctattcg cctcactaaa ggatagactt caccaattag
     1861 tcgacgtcct ccaccctttc taaagaactg aatcttcttt aggattaatt cttccttctt
     1921 ttcagaaaat ccaggaatat tcctaatctt accttctaat gcaacttttt ccaaatcaga
     1981 gatatttttg attcctagat tatcatacaa cgtctttatt gtttttggac caataccttc
     2041 caaaccgaaa aattggttga tatctattgg taacttctct tttagttcat cataataatg
     2101 tattttacct gtcgtgatat actcttcaag ttttgtagca atagcctttc caatcgaggg
     2161 gatttttagc aatcctttta gtttttcttc tttgtatatt tcgcctatat tggaagataa
     2221 attttgtatt tcatctgcag ccctattata tgatctaatt ttaaagattg cattaggttc
     2281 gcttttcttg tcttccaata tctgcaagat aaatgctatt tctcgaaaaa ttttggcagc
     2341 ttcagaattc gtgctcattg ttacattttc tctatataat actactagac ttaaaccaaa
     2401 cgctttgacg aaacattgaa ggtgcaatta aatcgtacaa taaatggcca gctcaaaaag
     2461 taaagtattt tattctttaa gttaatcatt ccatgttgtt atccagcgtt tatctggatc
     2521 atcactattt tttctttaat ttttgtaacc aaaaatgaag taatttggtt ttgtttttta
     2581 gctgataaaa agaaattgat attccaagaa atttaccata atctaaatag atttcaagtt
     2641 attgttttga tagttttctg atgtaccaat ccttttcata aactcataga attgcaataa
     2701 taattgctta ttctctgaat tgagaatatc tcttccaatg ttattagttg ttgtagatag
     2761 cttacctgtt gttttcttga acctcctatc tatgtatgtt aaaagacatg tatataaata
     2821 gaatatataa tggtcccatc gggagttgaa ttgacacggt tgcaatgttc aacatgctta
     2881 aatatttaat agttgataca atttatcata ataattacta gaaagtatac tctactacaa
     2941 tggagcaaac cagtaagtaa aa
//
LOCUS       DATAFW010000020         5427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5996041, whole genome shotgun sequence.
ACCESSION   DATAFW010000020 DATAFW010000000
VERSION     DATAFW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5996041"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..711)
                     /locus_tag="VHJ57_00375"
     CDS             complement(97..711)
                     /locus_tag="VHJ57_00375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF011463.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex kinase/ATPase Bud32"
                     /protein_id="HEX2487021.1"
                     /translation="MHYGIIKRGAEADVFLISWYKKVAISKLRNPKKYRNITLDNLIR
                     RRRTIHEANMLHDVKKTGIKTPFLYFVDPKRAEIIMEYIDGENVKDILSKEIAFIIGK
                     YVSTLHNKNIIHGDLTTSNFIKRKEDLYLIDFGLSFYSERIEDMATDIRIIKEILSSA
                     HIHIFEETYESFLEGYALVTNKNFNRILKVVKDIEARGRYARVV"
     gene            744..1070
                     /locus_tag="VHJ57_00380"
     CDS             744..1070
                     /locus_tag="VHJ57_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487022.1"
                     /translation="MSSIQPRKGEKLILQEDCSEDQLKNGLLTLTNQRLLFEKTEGTL
                     ATLSKRTEELLLDIPLVEIKASKPEGFVIKKIVVITIQNDIYKFGVFNNSKWAKEIEK
                     SKSNIQ"
     gene            complement(1101..2132)
                     /gene="kae1"
                     /locus_tag="VHJ57_00385"
     CDS             complement(1101..2132)
                     /gene="kae1"
                     /locus_tag="VHJ57_00385"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061711 - N(6)-L-threonylcarbamoyladenine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex N(6)-L-threonylcarbamoyladenine
                     synthase Kae1"
                     /protein_id="HEX2487023.1"
                     /translation="MKNNIKEDNIFCLGIESTAHTFGCSVIQFDLLKKKGKIISESRT
                     VYKPPDGAGIHPREASRHHLDKSPNILREVLIQANLKPKEIDLISYSAGPGLGPCLRV
                     GAVIARALSSFYNIPIIPVNHALGHIELGMMLTECRDPLVLLVSGGHTMVLAFSNKKW
                     RVFGETQDITIGQLLDQFGRFLGFASPCGNKIEKYANQSSKKYYHLPYIVKGNDVSFS
                     GILTAAKKLIEKKYNKFDICFSLQETAFAMLTEVVERALAFTEKKEVLIVGGVSANNR
                     LSTMLKIASKRQKATLYTCPLHFSGDNGAQIAWTAICEKVASGRAVKVQNALINQSWR
                     LDKVEVNWR"
     gene            complement(2136..3338)
                     /locus_tag="VHJ57_00390"
     CDS             complement(2136..3338)
                     /locus_tag="VHJ57_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX2487024.1"
                     /translation="MIIGVIGKANVGKSTFFNSATDLSVPSANYPFTTIHPNLGISYV
                     REKCVCREFGVQDNPQHSICIDGNRFVPIKLIDIAGLVPGAHLGKGLGNKFLDDARQA
                     DALIHVVDASGSTDPEGKVVEKSQGDPLFDIKFVEEEFDQWLLSIVNRDWNKTSREMT
                     NQKLKLEQILTKRLSGLSVSSDTISNALTTIGLINKNPDEWTQDDLLEFVRLIRKNSK
                     PLLILANKADVSSSQENIKKIKQQNNIVIPCASEGELVLRKGAKKGLWYYLPGDSNFE
                     LKDKDLLNEQQKKALLIIRSVLDKYRSTGVQEAINTVCFNLLNLIIVYPVEDEIKLSD
                     KKGNVLPNALLLRKGSTAKDLAINIHDDLAKGFMFAIDVRNKQRIGADYKLKHNDVIK
                     IVSTTSRG"
     gene            3494..4873
                     /locus_tag="VHJ57_00395"
     CDS             3494..4873
                     /locus_tag="VHJ57_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487025.1"
                     /translation="MAGIVAIYDFDAKRESTGEVLFYLVSSMRMVQHRGKAYWKMMVG
                     ERVLENKGSSPSNEVLSRLIKRKKLLGSNAIGYLSKRAPLFPSMDSIKIALDGFLVDT
                     EKLYLHPYIGSAKDADPLFQIYSIFIKLIDEKKDNSRAAEFLDRHLRGNLILKVKDSI
                     FVFRNSTGFKPLIMALAPDKALVIFSSENSLKISFSNFTYSDVKMGQLLKITSQNHIQ
                     ELTILERNKMLMDPFEFIRESHVTSTFNNKSIYTIRRNIGKIQANYLSKYMDLDTVYA
                     EPDYTRPMALGIGIELQKSILNFEISEGVIKDRYDDSDHMIDFSEEVSKNKLLTTGKS
                     LKFILENITKDKKVASIQGTIQTGSTAKETIYYLNRSGVKKIDVIVSYVPTIDGRQVG
                     LYTSNRDFIANKYMGKVTSIEKLNAYIADELVCEKTFYNSPYLLAKGIGVSENNLWFP
                     EWIRFLDYE"
     gene            4860..5204
                     /locus_tag="VHJ57_00400"
     CDS             4860..5204
                     /locus_tag="VHJ57_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487026.1"
                     /translation="MTTNNKRITIDVELNSLLQTLPKKSKIILSNITYDNNYFIKKIE
                     LLLYNTNNHKILGDYSILVIIIETKKGTVIMKYDEGYRGNDAFDNAIEMLTQFNGVSS
                     LINRALIELNSI"
     gene            complement(5229..>5427)
                     /locus_tag="VHJ57_00405"
     CDS             complement(5229..>5427)
                     /locus_tag="VHJ57_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487027.1"
                     /translation="DDLICVFDSIPKSIYPFDILIDDAPQNIVDIKFPKKGILFDQPW
                     NRRFNWPIRINRLSDLFHYLP"
BASE COUNT         1876 a          856 c          772 g         1923 t
ORIGIN      
        1 aaaaaatatt gcattcattt gaattttatt atcgtaaatc ttatttaata gattacagac
       61 aacaaaatga ttatctatct tctgtacctt attttattaa acaactcttg catatcttcc
      121 tcgtgcttcg atatctttga ctacttttaa aattcggtta aaatttttat tagtaactaa
      181 tgcatatcct tccaaaaagc tttcgtatgt ttcctcaaaa atatgtatat gtgcactact
      241 taaaatttct tttattatcc taatatcagt agccatgtcc tcgattcgct ccgagtaaaa
      301 agaaagacca aaatcgatta aataaagatc ctccttacgc tttataaaat ttgaagtagt
      361 caaatctcca tgtattatat ttttattatg aagagtagaa acatatttac ctattataaa
      421 tgcgatttcc ttcgaaagta tatcttttac attctcccca tcaatatatt ccattataat
      481 ttcagcacgt tttggatcaa caaaatatag aaaaggagtt tttattcctg ttttctttac
      541 atcatgtaac atattagctt catgtatagt cctccgtctt cttattaaat tgtccaatgt
      601 tatatttcta tattttttag gatttcttaa ttttgaaatt gccacttttt tataccatga
      661 tattaaaaag acatccgctt ctgctcctct ttttataatt ccataatgca atgataatat
      721 atcgatctaa taagaataaa aatgtgagtt ctatacaacc aagaaagggt gaaaaactaa
      781 ttcttcagga ggattgttct gaagatcaat taaagaatgg attattaaca ttgacaaatc
      841 aaagattact ttttgaaaaa actgaaggga ctctagcaac tctttccaaa cgaacagagg
      901 agttattact tgatattcct ttagtagaaa ttaaggcatc aaaaccagaa ggatttgtta
      961 taaaaaaaat agtggttata acaatacaaa atgatatcta taaattcggt gtgttcaata
     1021 attctaaatg ggcaaaagag atagaaaaat ctaaatccaa catacaatga ttctaactat
     1081 attgtaagaa acttttttct ctatctccag tttacttcta ctttgtctaa tctccatgac
     1141 tgatttatca atgcattttg aactttaact gctctacctg aggcaacctt ctcacatata
     1201 gctgtccatg caatttgagc tccattatca cctgaaaagt gtagaggaca ggtatacaat
     1261 gttgcttttt gccttttaga ggctattttt aacatcgtag aaagacggtt atttgcagaa
     1321 actcctccta ctatcaaaac ttcttttttt tctgtaaaag ctaatgctct ttcaacaact
     1381 tcagttaaca ttgcaaatgc agtttcctga agtgaaaaac aaatgtcaaa tttattatat
     1441 tttttctcta tcaatttttt agccgcagtt aagataccag aaaatgaaac atcattacct
     1501 tttacaatat agggtaaatg ataatatttt tttgaagact ggtttgcata tttttcaatt
     1561 ttatttccac aaggggaagc aaaacctaaa aatcttccaa attgatctaa taattgtcca
     1621 attgtgatat cttgggtttc gccaaatact ctccattttt tatttgaaaa tgctaatacc
     1681 atggtatggc ccccagacac taataaaacg agaggatccc gacattctgt aagcatcata
     1741 cctaattcga tatgccctaa agcatgattt actggtatta ttgggatatt ataaaatgat
     1801 gataacgctc tggcaataac cgcgccaact ctaagacaag gtcccaaccc aggacctgcc
     1861 gaatatgaaa ttaagtctat ttctttaggc tttaaattag cttggattaa tacttccctc
     1921 aaaatattag gagacttatc caaatgatgt ctagaagcct cccgtgggtg tatacctgct
     1981 ccatcaggag gtttatacac agttctcgac tctgatatta ttttaccttt tttctttaac
     2041 agatcaaatt gaattactga acatccaaaa gtatgagcag tactttcgat tcccagacaa
     2101 aaaatattat cctcttttat attatttttc ataatttatc ctctactagt ggtagaaact
     2161 atttttatta catcattatg tttcaatttg taatctgccc caattctttg tttatttctt
     2221 acatcaatag cgaacataaa tccttttgct aaatcgtcat gaatatttat ggccaaatct
     2281 tttgctgttg atccttttcg taataaaagt gcattcggaa ggacatttcc ctttttatca
     2341 gataatttta tttcgtcttc aactggataa actatgatta gatttagtaa gttgaaacat
     2401 actgtgttta tagcttcctg aactccagta gatctatatt tgtctaatac agatcttatt
     2461 attaataatg cttttttttg ttgttcattt aagagatctt tatcttttaa ctcaaaatta
     2521 gagtctcctg gaagataata ccataaacct ttttttgccc ctttcctaag aactaattca
     2581 ccttctgagg cacatggaat tactatatta ttctgctgtt ttattttttt tatattctct
     2641 tgggaagaag aaacatccgc tttattagct aatattagta aaggctttga attttttctt
     2701 attaatctta caaactctaa taaatcatct tgagtccatt catcaggatt tttattaatc
     2761 aatcctatag tagtcaatgc atttgaaatt gtatcactac tgacagataa tccagagagt
     2821 ctttttgtta gtatttgctc aagtttcaat ttttgatttg tcatttccct ggaagtttta
     2881 ttccaatctc tgttaacaat tgaaagaagc cattgatcaa attcttcttc gacaaattta
     2941 atatcaaaga gaggatcacc ctgagacttt tcaacaactt taccctcagg gtcagttgaa
     3001 cctgatgcgt cgactacatg gattaatgca tctgcttgtc ttgcatcatc tagaaattta
     3061 tttcccaaac cttttcctaa atgagcacca ggaactagtc ctgcaatgtc tattaatttt
     3121 ataggtacaa acctatttcc atctatacat attgaatgtt gtggattatc ttgaactcca
     3181 aattccctac atacacattt ttctctgaca taacttatac ctaggttagg gtgaatagta
     3241 gtaaaaggat agtttgcgga aggaacggat agatcagtag cagaattgaa aaaagtagat
     3301 ttgccaacat tagcttttcc gataactcct ataatcataa taatattaag tcataatagc
     3361 actattagta ttttttattt tttattataa tttaagaatc actgccgtct gcgaataaat
     3421 tttaattctt aaacttttat cttataaaat ttttatatat ttaatacaac ctctttataa
     3481 aagttatagg accttggctg ggatagtggc aatttatgat tttgatgcta aaagagaatc
     3541 tacaggtgag gtcctttttt atcttgtaag ctcaatgcgg atggttcaac atagaggaaa
     3601 ggcatattgg aagatgatgg tgggggaaag agttttagaa aataaaggat ccagtccatc
     3661 aaatgaagtt ttatcaagat taataaaaag aaagaaatta cttggttcca atgctattgg
     3721 ttatctttcc aaacgtgctc cattatttcc cagtatggat tctattaaaa ttgcgttaga
     3781 cggttttctg gttgatactg aaaagttata tcttcatcca tatataggat ctgcaaaaga
     3841 tgcagacccc ttatttcaga tatattccat atttattaaa ttaatagatg agaaaaaaga
     3901 taatagtcgt gccgcagaat ttttggatag acacctgaga ggaaatctca ttctaaaagt
     3961 aaaagattca atttttgttt ttcgaaatag taccggattt aaaccattaa taatggcatt
     4021 ggcaccagat aaggctttag taattttttc atcagaaaat tccttgaaaa tttctttttc
     4081 taactttaca tatagcgatg taaaaatggg gcaattgctt aaaattacta gccaaaatca
     4141 catacaagaa ttaacaattc tcgaaagaaa taaaatgtta atggaccctt ttgaatttat
     4201 tagagaatct cacgtaacaa gcacctttaa caataagagt atctatacaa taagaagaaa
     4261 tattggaaaa attcaagcca attatctatc aaaatatatg gatctagata ccgtatatgc
     4321 tgaacctgat tatactagac ccatggctct tggaatagga atagaattac aaaaatctat
     4381 tttaaatttt gaaatctctg aaggagtaat caaagataga tatgatgact cagatcatat
     4441 gattgatttt tcagaggagg taagtaaaaa taaattgctt actaccggaa aatccctgaa
     4501 atttatttta gaaaatataa ctaaggataa aaaagtggct tcaattcaag gaactattca
     4561 aacaggtagt actgccaaag aaacaattta ttatttaaat agaagcggag taaaaaaaat
     4621 agatgtaata gttagttatg ttcctactat tgatggacga caagttggtt tatatacttc
     4681 taatagagac tttattgcta ataaatatat gggaaaagtt acctctatag agaaacttaa
     4741 tgcttacatt gctgatgaac ttgtatgtga aaaaacgttt tataattcgc cctatttatt
     4801 ggcgaaagga ataggcgttt cagaaaataa tttatggttt ccagaatgga tcaggttcct
     4861 tgactacgaa taataaaaga attactattg acgtagaact taacagttta ttacaaacac
     4921 tccctaaaaa gtccaaaatt attttaagta atattacata tgataataat tattttataa
     4981 aaaaaataga attattgtta tataatacaa ataatcataa aatactcgga gattattcaa
     5041 ttttagttat tatcatcgaa acaaagaagg gaactgtgat aatgaaatat gatgaaggat
     5101 atcgtgggaa tgatgctttt gataacgcaa tagaaatgtt aacacaattt aatggggtgt
     5161 catccttaat taatagagca ttaattgaat taaatagcat ttaatttttt tatgttattc
     5221 ctctaaaatt acggtagata atgaaataaa tctgataacc ggtttattct aattggccaa
     5281 ttaaaccttc tattccaggg ttgatcaaat aaaatccctt tctttggaaa ttttatatca
     5341 actatatttt gaggtgcatc atcaattaaa atatcaaagg gataaatact ctttggaata
     5401 gaatcaaaaa cacatattag atcatcg
//
LOCUS       DATAFW010000021         5012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4234629, whole genome shotgun sequence.
ACCESSION   DATAFW010000021 DATAFW010000000
VERSION     DATAFW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234629"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHJ57_00410"
     CDS             <1..333
                     /locus_tag="VHJ57_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HEX2487028.1"
                     /translation="QLIINQLSGKYKVKSEKLKIYYEKTKILIKKFENIQIEWIAREN
                     NNEADALANKAYINFIDNNYARLHNKLKPYFATDQQITLLKHLKIKPSKYLSRIEANR
                     YLKKYNQK"
     gene            396..833
                     /locus_tag="VHJ57_00415"
     CDS             396..833
                     /locus_tag="VHJ57_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487029.1"
                     /translation="MPRTKSSEKSSEKVGIPQWIEEEIKSIQFTESEIINRTGYILEI
                     YLDDYKLDIQLYEALPDGRTIVEGINVPSSQDTSKFMKGFVYEFKIKMSRGKLSNKTI
                     EFLKSQFLLEMDSIYQFELIELHLMDVESEESSQQSLDDTEDL"
     gene            complement(883..1917)
                     /locus_tag="VHJ57_00420"
     CDS             complement(883..1917)
                     /locus_tag="VHJ57_00420"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX2487030.1"
                     /translation="MNWRLIAVLVSFLPVILLFIFSPISITDIFSVGIAPFITAVLFS
                     LAKVFLQAYRFKYFVDQFLGYKIASSVKLISVRIGSEFVTNTTPSYIGGEIVRIAWLT
                     KNKVPAGIAAWITTMEIIADVFVGTILALFAGIIALANGAILIGTIIILISSFIFGFW
                     LIVVILSAYYNIQLPKVITKLVRQFLSKARSDKIINYTNSAISDLCAMSRENFNSTRT
                     IKIFFVGMVLTFIAFACMGASFLVLGGLIESDLGLYDSILATAASTELGSLPITVGGS
                     GLTEIGLWAYVSGLNKLPSLNEMMNDSHLDVIISWRIASYHIPLIIMWLVLMRLTGIK
                     KLTSRSNQRS"
     gene            2040..2798
                     /locus_tag="VHJ57_00425"
     CDS             2040..2798
                     /locus_tag="VHJ57_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEX2487031.1"
                     /translation="MKAIYITGTAGSGKSLLTSNLLQWYIDHDNFPIALNLDPAVSEL
                     PYFPDVDIRDHIDINNIIDTYGLGPNGSIIMSYDLIGTKIQDIQLEVNELNPDYILID
                     TPGQIELFAYRSSGNYFISNFQADDKASIFIMDGVLVSSPINFVSLSLLSASINLRLK
                     TSQINVLSKRDLILDNLENILDWAHSDTALLKALDKENNPEFSLLSKDIIKIISQNGL
                     NQELLAISNLTLNGLIELTSVLSRILNQGEEIEY"
     gene            2810..3772
                     /locus_tag="VHJ57_00430"
     CDS             2810..3772
                     /locus_tag="VHJ57_00430"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX2487032.1"
                     /translation="MNSFRALAPSSTANLGPGFDIFGLALDLFVDEIIITRKQEQNGR
                     ITISTIKQNDSMKIPYDNENNSAALVVKKMMKDYSIKDNIDIQINKGVPPGYGLGSSA
                     ASSVATAYAFDKLFHLETNLIDLISYSAEGEKASAGAKHYDNVASSMLGGFIIVRNFP
                     SLEFIKFEPPEDLYLVVGIPNIPVPNKKTEVARSILPVSSPLDNVITNISNASTIVAG
                     FACKDVNMIAKGLDDKIVEPVRKRTIPGYDKVKENAINSGALALTISGAGPSVISILN
                     TERHAHKVMEAVKGGFAAANIESKVYLCKPSKGAKNQDKISSSY"
     gene            complement(3774..4634)
                     /locus_tag="VHJ57_00435"
     CDS             complement(3774..4634)
                     /locus_tag="VHJ57_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX2487033.1"
                     /translation="MSSMKYVLLEPIGNIAIIKINRPEALNAMNVDVIWELSKTIDIV
                     GVDDSIKAVIITGVGDRSFCAGADISYMVNITPSDAEKYASSAQSVLNKIEKLEKPVI
                     AAINGFALGGGCELALVCDIRLCSTNAKIGQPEVTIGIPPGWGGTQRLLRIVGPAKAK
                     EMIFTGKMINAEEAYKIGLVNDVINLTQEELPEAPPEGEEVKRKEYQKELAKLLNKKL
                     IEKSVSMAQEITNNSYNAIKASKYLINKGMDMDIDSGLRMEIYGWALCFAHEDRKNLM
                     SAFLSKGKKK"
     gene            complement(4763..>5012)
                     /locus_tag="VHJ57_00440"
     CDS             complement(4763..>5012)
                     /locus_tag="VHJ57_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2487034.1"
                     /translation="PFCAFNVIPEWYRDRIQKKYSIPVEEWEKAHGQTLEAGLYRGLM
                     RRGKPEAQMGCAVSEMHKAAAESEDHRAVDLRTSMAGA"
BASE COUNT         1815 a          827 c          741 g         1629 t
ORIGIN      
        1 caattaatta ttaaccaact ctcaggtaaa tataaagtaa agtcagaaaa attaaagatc
       61 tactatgaga aaacaaaaat attaataaaa aagtttgaaa atatacaaat agaatggata
      121 gctagagaaa ataataatga agccgacgcc ttagcaaata aagcatatat caattttata
      181 gataataatt atgcaagatt acacaacaaa ttaaaaccat attttgcgac ggatcaacaa
      241 attacccttt taaaacattt aaaaatcaag ccctctaaat acttgagtag aattgaggca
      301 aatagatatc tcaagaaata taatcaaaaa tgatactttt tattcaattc aatttatgca
      361 aagcttttat ctagtttgtt tattgtatat tttgaatgcc ccgaacaaag agttcagaaa
      421 aatcttcaga aaaggtaggg attccacaat ggatagaaga agagattaaa tcaatacagt
      481 ttacagaatc tgaaataata aatcgaacag gctatatttt ggaaatttat ttggatgact
      541 acaaactaga catacaactt tatgaagcat tacctgatgg aaggactata gtagaaggca
      601 taaacgtacc atcttcacaa gacacatcaa aatttatgaa gggtttcgtt tatgaattca
      661 aaattaaaat gtcgcgagga aagcttagca ataaaactat agaatttctc aaatcacaat
      721 tcttgttaga aatggattcc atatatcaat ttgagttaat tgaattgcac ttgatggatg
      781 tagaatctga agaaagttca caacaatcac ttgacgatac agaagacctg taataacata
      841 gacattggaa aatgtatttt tcttataaga atgaattatt ggtcaagacc tttgattaga
      901 tctagatgtc aattttttaa tccccgtaag tctcattaac acaagccaca ttataataag
      961 aggaatatga taagatgcaa ttcgccaaga aataattaca tctaaatgtg agtcattcat
     1021 catctcatta agtgaaggca atttattaag accagagacg tatgcccaca acccaatttc
     1081 agttaagcct gatcctccga ctgttatagg tagactacct aattcagttg aagctgcggt
     1141 agccaagata gagtcatata atcccagatc agattctata aggcctccaa gtacaagaaa
     1201 ggatgctccc atacaggcaa atgcaataaa tgtaagaacc attccaacaa agaatatttt
     1261 gattgttcgt gttgaattga agttttccct actcatggca cataaatcac taattgcgga
     1321 attggtgtag ttaataattt tgtcagaacg tgcctttgac aaaaattgtc ttactaattt
     1381 tgttataact ttaggaagct gaatattata atacgctgaa agaataacaa ctatcaacca
     1441 aaacccaaaa ataaatgaag aaataagaat aattatagta ccaataagaa ttgccccatt
     1501 agcaagtgca attattcctg caaataatgc aagaattgtt cctacaaaaa catctgcaat
     1561 aatttccatt gttgtaatcc atgctgcaat tccagctgga actttgtttt tagttaacca
     1621 ggcaattcga acaatttctc ctccaatata agagggagta gtattggtaa caaattcact
     1681 tcctattctt acggaaatta atttaacgga cgatgctatt ttgtacccta aaaattgatc
     1741 tacaaaatat ttaaaacgat atgcctgaag aaagactttc gcaagactaa ataaaacagc
     1801 tgtaataaac ggagcaattc caacactaaa gatatcagta atagatattg gagagaaaat
     1861 aaataaaaga ataactggta aaaaacttac taatactgca atgagtctcc aattcaattt
     1921 atctgattac taaactaata cagttatata ataatcaatc aatttaaaat taataaaacc
     1981 gttacattgc tattataaag tataaaatat taataattat tatttaaata caaaaataaa
     2041 tgaaagccat ttatataaca ggaaccgctg gttcaggaaa atccttactt acttctaacc
     2101 ttttacaatg gtatattgac catgataatt ttcctattgc ccttaatttg gaccccgctg
     2161 tatctgaatt accttatttc cctgatgtag atatacgaga ccatatagac attaataata
     2221 ttatagacac ttatggttta gggccgaatg gttctataat aatgtcgtat gatttaattg
     2281 gcacaaagat tcaagatatt caattagaag ttaatgaatt aaatccagat tatatcttaa
     2341 ttgatactcc cggacaaatt gaattgtttg cttatagatc tagtggtaat tattttattt
     2401 caaattttca agcagatgat aaagctagta tttttataat ggatggtgta ctagtttcaa
     2461 gcccaattaa ctttgtttcc ttatcattac tttctgcttc gattaatctt agattaaaaa
     2521 cctcacagat aaacgtatta tcaaaacggg atttaatact agataattta gaaaatatct
     2581 tagattgggc tcattctgat acagctttat tgaaagcttt agataaagaa aataatccgg
     2641 aattttcttt attaagtaaa gatatcataa aaattatttc gcaaaatgga ctaaatcaag
     2701 aattgctagc tatatctaat ctgactctga atggtttaat agaattaact tcagttttat
     2761 caagaatact taatcaagga gaagaaattg aatattaaat agataagaaa tgaattcatt
     2821 tagagcactt gcgccttcat ccacagctaa tttaggacct ggtttcgata tatttggtct
     2881 tgctttagat ctctttgtag atgaaataat aataactagg aaacaagaac aaaacggaag
     2941 aatcactata tctacaataa aacaaaacga ctctatgaaa attccttatg ataacgaaaa
     3001 caattctgca gcattggtag ttaagaagat gatgaaagat tatagtataa aagataacat
     3061 tgatattcaa atcaataaag gagttcctcc aggttatggt ctaggaagta gcgcggcttc
     3121 atcagtcgcc acagcgtatg cttttgacaa gttatttcac ctagaaacaa atttaattga
     3181 tttaattagt tattctgcag aaggcgaaaa agcttcagca ggagctaaac attatgataa
     3241 cgttgctagt tctatgttgg gaggatttat aatagtgaga aatttcccat cacttgaatt
     3301 catcaaattt gagccacctg aagatcttta tcttgtagtt ggaattccca acataccagt
     3361 tccaaataag aaaacagaag ttgctagaag tatactacct gtgagttcac ctttggataa
     3421 cgtcataact aatatttcta atgcaagtac cattgtggca gggtttgcct gtaaagatgt
     3481 gaatatgata gcaaagggac ttgatgataa aattgtagaa cctgttagga aaagaacaat
     3541 tccaggctat gataaggtaa aggaaaatgc tattaattca ggtgcattag ccctcactat
     3601 tagtggtgct ggtccttcag ttatttcaat cttgaataca gaaagacatg ctcacaaagt
     3661 tatggaagca gttaaaggag gatttgcagc tgcaaatatt gaaagcaagg tttacttatg
     3721 taaaccaagt aaaggggcta aaaatcaaga taaaatatct tcatcttatt aactcatttc
     3781 tttttacctt tactaagaaa tgcagacatt agattttttc tatcttcatg agcaaagcaa
     3841 agagcccatc catatatttc cattctgaga ccggaatcta tatccatgtc catgccttta
     3901 ttaatcaaat atttacttgc tttaatagca ttataactat tgtttgtaat ttcttgtgcc
     3961 atagaaacag acttttcaat taacttttta tttaaaagtt ttgccaattc cttctggtat
     4021 tctttacgtt ttacttcttc tccctcagga ggtgcctcag gtaattcttc ttgtgttaaa
     4081 ttaataacgt cgttaactaa tcctatttta tatgcttctt ctgcatttat catttttcca
     4141 gtaaaaatca tttcttttgc tttagctggg cctacaatac gtagcaatct ttgtgttcct
     4201 ccccatcctg gtgggattcc tattgtaact tctggttgac caatttttgc atttgttgaa
     4261 catagtctaa tgtcacatac tagtgccagt tcacatcctc cacctaatgc aaaaccatta
     4321 attgcagcta ttactggttt ttccaatttt tcaattttat ttaagactga ttgagcagag
     4381 gaagcatatt tttccgcatc actaggtgtg atgttaacca tataagatat atctgcacct
     4441 gcacaaaatg atctatctcc aacgccagtt attataacag cttttatact atcatctact
     4501 cccacaatat caattgtttt agataactcc caaattacat ctacattcat tgcattcaac
     4561 gcctcaggtc tgttgatctt aataattgct atattaccta taggttctaa gagaacatat
     4621 ttcattgaag acatattctt attccaataa ctaacatcaa ttaaacttta ttaaaaaaat
     4681 agtataaata ttgttaaaaa aaataatttt tgtagaaata cttaaactaa ttaatattac
     4741 taatctgaaa taaagttgaa gattaagctc cagccatgct agttcttaaa tctactgctc
     4801 gatgatcttc gctttcagcg gctgctttat gcatctctga aacagcacaa cccatttgtg
     4861 cttctggttt tccacgtctc attaatccac ggtataatcc agcctctaaa gtctgtccat
     4921 gtgctttttc ccattcttct actggaatac tatatttctt ttgtattcta tcacgatacc
     4981 attcaggtat aacattaaaa gcacagaatg ga
//
LOCUS       DATAFW010000022         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6241, whole genome shotgun sequence.
ACCESSION   DATAFW010000022 DATAFW010000000
VERSION     DATAFW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6241"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VHJ57_00445"
     CDS             <1..749
                     /locus_tag="VHJ57_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487035.1"
                     /translation="ITTSFLVPEKSIDYWKDRLNSNDICFEGPKKRFENEQVITLYDP
                     DGLQLELVAHSSARDNDERFWKDGPISYENGIRGFYSVSLSEEGYELTSEILNELGYK
                     MIENEGNRFRFQNTDSKSTGAKVLDVLCLPYTHPGLIGIGTVHHIAFSTSSDENQILV
                     RKSIIKKGLNPTPVIDRTYFHSVYFREPGEILFEIATDPPGFTIDQKIEDLGRKLMLP
                     TWLEPLRESLEKVLPKIELSKNKDSLRISD"
     gene            755..1411
                     /locus_tag="VHJ57_00450"
     CDS             755..1411
                     /locus_tag="VHJ57_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2487036.1"
                     /translation="MIKSNNFGFNHIFINSSHDKEYEEGNKKLTLVLLHGTGGNEEDL
                     IFLGKKIEPNASILSPRGKVLENNMPRFFRRLSEGVFDIEDLHVRSHELADFIQKCSF
                     HYKFNLEKTIAIGFSNGANIAVSMLFLRPEVLQGAILFRAMVPFIPESLPNLLNKKIL
                     LSAGVQDPIVSRNETENLYSLFQKTNAITTLKWQPSSHNLIQKDILVAKSWISNNFPV
                     "
     gene            1770..2195
                     /locus_tag="VHJ57_00455"
     CDS             1770..2195
                     /locus_tag="VHJ57_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2487037.1"
                     /translation="MLTEPVVKIIKDKNFAFLGTIMKDGRPQVSPIWIDIDDNNNIIL
                     FNTAQGRIKHKNISRDPRISLSLINKNNPYSMVTIQGTVIEQTTIGADEHIDKLAKKY
                     LNTDRYPGHSPSVKRVICKIKPEKMYYLPPRYTEFIGKS"
     gene            2503..3408
                     /locus_tag="VHJ57_00460"
     CDS             2503..3408
                     /locus_tag="VHJ57_00460"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX2487038.1"
                     /translation="MTTNLWPALPLGEWKDTYTTLHMWTQIVGKIRLALTPFRNHWWN
                     TTLYVTPRGLTTSAMFYNDRLLQIDFDFIDHLLVIQTDEGSTKRIPLRPRSVAEFYKE
                     TMAALSSLGMPVTIWTTPVEVPDRTPFDEDFKHIDYDPEYAQRVWRILAQGSRILSLF
                     SSRFIGKVSPVHFFWGAFDLAVTRFSGRKAPSHPGAPNMARFVAVEAYSHEVSSCGFW
                     PGGGSIDEPVFYAYAYPEPQGFKDYSIQPADAFYHEGMGEFLLPYDIVRRSDSPDKIL
                     LSFLQSSYEAAAICANWDRHILERQ"
BASE COUNT         1230 a          534 c          578 g         1090 t
ORIGIN      
        1 ctataacaac atctttttta gtacctgaaa aatctattga ttattggaag gatcgtctaa
       61 atagtaacga tatttgtttt gaaggtccta aaaaacgttt tgagaatgaa caggtaataa
      121 cgctatacga tcctgatggc cttcaattgg aacttgttgc tcactcatca gcaagagata
      181 atgacgaaag attttggaaa gatggtccaa tctcttacga aaatggtata agaggatttt
      241 attctgtaag tttatctgaa gaaggatatg agcttacctc agaaatttta aatgagttag
      301 gatacaaaat gatagaaaat gaaggtaacc gtttccgctt tcaaaataca gattcaaaat
      361 ctactggagc taaagtgtta gatgttcttt gtttgccata tactcatcct ggactgatcg
      421 gaataggaac tgttcatcat atagcattta gtacttcttc agatgaaaac caaatattgg
      481 ttaggaaaag tattataaaa aaaggcttaa atcctacacc tgtaattgac agaacctatt
      541 ttcattcagt atatttcaga gaaccaggtg agattttatt tgaaattgct acagatcctc
      601 caggatttac tatagatcaa aaaatagaag atttaggaag aaagctaatg ttacccacgt
      661 ggcttgaacc attaagagaa agtttagaaa aagtattgcc aaagatagaa ctatctaaaa
      721 ataaagatag tttaaggata agtgactagc aataatgatt aaatccaata attttggttt
      781 taatcatata tttattaatt cttctcatga caaagaatat gaagagggaa ataagaaatt
      841 aacacttgta ttattacatg gtacaggtgg aaatgaagaa gatcttattt ttcttggaaa
      901 gaaaattgaa cctaatgcat ctatattaag tcctagagga aaagtattag aaaataatat
      961 gcctaggttt tttagacgat tatctgaggg agtctttgat atagaagatc ttcatgttag
     1021 atcacacgaa cttgcggatt ttatccaaaa atgttccttt cactataaat ttaatttaga
     1081 aaagacaatt gcaattggat tttcaaatgg agcaaacata gctgttagta tgctctttct
     1141 tcgtccagaa gtacttcaag gagcaattct tttcagggca atggttcctt ttattcctga
     1201 atccctacca aatcttttaa acaaaaaaat actattatct gcaggggtac aagatccaat
     1261 agtttcgaga aatgagacag aaaatcttta tagtttgttt caaaagacta atgcaatcac
     1321 aacattgaag tggcaacctt ccagtcacaa tctaattcaa aaggatatac tagttgcaaa
     1381 atcatggata tcaaataatt ttccagtttg aataaaatag aaaaggatat taatttattt
     1441 taaaaattac tattttataa tctgattctt ctgacatttg ttctaatatt gatttgtttt
     1501 ttccactaga gcagataaat attattattt ttataataat ataatttcta tattttgata
     1561 tttcttatac agattagaat aatgtttctt atcattatat atattctaaa ttattatctg
     1621 aaaaaaacaa aacatcatta ttgacatata atctaaatat agtatcctat tatcactagt
     1681 aagttaaagt aattgaacac ctataaggaa caatctttga taataattta aaacatgaat
     1741 attattgatg aggacaaaga aaaaaaagga ttctcactga accagtagta aaaataataa
     1801 aagataaaaa tttcgctttt ttgggaacaa taatgaaaga tggtagacca caggtatctc
     1861 ctatttggat cgatattgat gacaataata atattatttt attcaataca gcccagggaa
     1921 gaataaaaca taaaaatatt tcgagagatc ctagaatatc tctatcactt atcaataaaa
     1981 ataatccata tagtatggta acaattcaag gaactgtcat tgaacagaca acaataggag
     2041 ctgatgaaca tatagacaaa cttgcaaaaa aatatcttaa tactgataga tacccgggtc
     2101 attcaccttc agtcaaaagg gtgatatgta aaattaaacc cgagaaaatg tattatttac
     2161 ctccaagata tacagagttt attggaaagt cttaggtcta ttttattaat actattaaaa
     2221 accatatagt agcaggttga attgaacatt taatttaatt atatccaata agttagatgt
     2281 ttacaattaa aaattataat tataataata ataacaatct tattaataga tataaatgaa
     2341 cgataatagc aattttatac atagattact taaatccatg agagcacatt tacaagctaa
     2401 gatcatactc atgaaggtta ataatcgaat ttgcagtacc cataagcttt atcttatctt
     2461 aagtgatccg tctcagagtt ggtgccgaaa gggagtaaat caatgactac caacttatgg
     2521 ccagcattac cgttggggga gtggaaagac acctatacca cattgcatat gtggacacaa
     2581 atagtaggca agatccgact tgcacttact ccattcagga atcactggtg gaatactaca
     2641 ttgtatgtta cacctagagg acttacaacc tcagctatgt tctacaatga tagactactt
     2701 cagatagact ttgactttat tgatcacctg cttgttatcc aaacagatga aggttctacc
     2761 aagagaatcc ctcttagacc acgatcagta gctgagtttt ataaagaaac gatggcagcc
     2821 ttgagttcac tagggatgcc tgtaaccatc tggacaacac ctgtcgaagt accggatcgt
     2881 actccatttg atgaggattt taaacatata gactatgatc ctgaatatgc acagcgtgtc
     2941 tggcgtattc tggctcaagg aagtcgtata ctctctttgt ttagttcccg atttattggt
     3001 aaagttagcc cagtacattt tttctgggga gcttttgatc tggcagtaac ccgattttca
     3061 ggacgaaaag cgccttcgca cccaggtgca ccaaatatgg ctcgttttgt ggcagtagaa
     3121 gcatattcac acgaagtgag tagttgcggt ttctggccag gtggaggttc tatagatgaa
     3181 ccagttttct atgcctatgc ttatcctgaa cctcaaggat ttaaggacta ttctatccaa
     3241 ccagcagacg cgttctacca tgaaggaatg ggagaattcc ttcttccata tgatattgtt
     3301 cgaagatccg attcacctga caaaatatta ttgtctttct tacagagcag ttatgaagca
     3361 gcagcaatat gtgctaactg ggatcgtcat atattggaac gtcagtagat agtaaaatta
     3421 attccttgtg tc
//
LOCUS       DATAFW010000023         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1417459, whole genome shotgun sequence.
ACCESSION   DATAFW010000023 DATAFW010000000
VERSION     DATAFW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417459"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VHJ57_00465"
     CDS             complement(<1..574)
                     /locus_tag="VHJ57_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2487039.1"
                     /translation="MEIDSGMFLVLLGPSGCGKTTIIKSIAGLIDPTEGQIYIDNTLI
                     NKLPPKNRDVAMVFQSYALYPHMSVYDNIAFPLKMRKLPKHEIKDKVANTASLLELTQ
                     FLERKPKELSGGQMQRVALARALVRKPKLFLMDEPLSNLDAKLRMQMRTEIKKLQKKI
                     RITTIYITHDQIEAMSMADQIIVMNKGVIQQ"
     gene            918..>2114
                     /locus_tag="VHJ57_00470"
     CDS             918..>2114
                     /locus_tag="VHJ57_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX2487040.1"
                     /translation="MNFIPVIAQEEITLTAIFAEPKERWDMLLENATKILNERHPDKT
                     IKIDYRVLPYSDTRTQILTAMAGKTPIDLISLDQIWLGEFAEGGFLSDLTANTQKWNK
                     SSDWYLQNWEGGLYNDQVKGIWAWTDVRALWYWKDLLGKAEINPDELKNWNGYLSASK
                     KLHQVLLKDGTQGMHLVGAAHSPDMWYPYLWMLGGEILEKREGHPTKQNYWYPTYNSD
                     LGIKALQFLKDQVNAGIKPQMNHYWGQEFADKKFAVMLEGSWLLGQFDKNEWNTLKEK
                     IGMIPMFPVPNTLNTNTSTLMGGWLLSIPETSQNKEISWELLTIMLEPNVLIPMLLQQ
                     GYLPTQKSIGEGQYSEELKSTIPYYEELISLIELGKGRPNIPEYPIVAEHIRNAIEQV
                     YNGMDPK"
BASE COUNT          734 a          338 c          355 g          687 t
ORIGIN      
        1 tctgctgaat tactcctttg ttcataacga taatttgatc ggccatactc atagcttcaa
       61 tttgatcgtg agtgatatag atggttgtaa ttcttatttt cttttgtaat ttcttaatct
      121 ccgttctcat ttgcatacgt aacttagcat ccaaattact cagtggttca tccattagaa
      181 ataactttgg ttttctaaca agtgctcttg ctaaagcaac tctttgcatt tgaccgccgc
      241 tcaattcttt tggctttcgt tcaagaaact gagtaagttc taatagacta gcagtattcg
      301 ccaccttatc ttttatttca tgttttggta attttctcat ttttaaagga aatgcgatat
      361 tatcatatac gctcatatgt ggatataatg cataactttg aaaaaccata gcaacatcac
      421 gatttttggg tggaagttta tttattaaag tattatcaat gtaaatttgt ccttctgtag
      481 ggtcgataag cccagctata gattttatta tagtagtttt accacagccc gaaggaccta
      541 atagtactaa aaacattcct gaatcaattt ccacagaaaa tttcgttact gcatttacac
      601 ttccatacct tttactaaga tttttgattg ttattctaga cagaaaatac tcactaagtc
      661 tactaaaaag taatatttaa tattaatttg taacaataaa caactcaata aattattata
      721 ctagacaaca gaaaatacgc attctataat agaatcgtat attgtattaa ataaacaacc
      781 ttgaaaataa tcatatttat tagtaaataa ttaataatag tagtagttgt aacttctgtt
      841 ataattctaa aatggccaag ttaaatcttt taattacagt atcttttgca attttatctt
      901 tattttttat ttcacaaatg aattttatcc ctgttattgc tcaagaggag ataactctta
      961 ctgcaatttt tgctgaacct aaggagcgat gggatatgct cttggagaat gctacaaaga
     1021 tattaaatga gagacatccc gataaaacta ttaaaattga ttatagagta ttgccatatt
     1081 ctgatacacg aacacaaatc cttacagcaa tggcaggaaa aactcctatt gatcttattt
     1141 ctttagacca aatatggctc ggtgaatttg cagaaggagg atttttatca gatcttacag
     1201 ctaatacaca aaaatggaac aagtcctccg actggtatct acaaaattgg gagggaggat
     1261 tgtataatga tcaagtcaaa ggcatttggg catggacaga tgtcagagca ttatggtatt
     1321 ggaaggatct attgggtaaa gcagaaatta atcctgacga gctaaagaat tggaatggat
     1381 atttatctgc atctaaaaaa ttacaccagg ttttactaaa agatggaact cagggaatgc
     1441 atttagtagg tgctgcccac tcacctgata tgtggtaccc ttacctatgg atgctcggag
     1501 gggaaatatt agaaaaaaga gaaggacacc caacaaaaca aaattattgg tatcccacat
     1561 ataacagtga tttaggtatt aaagctcttc aatttttaaa agatcaagtt aatgctggaa
     1621 ttaaacctca aatgaatcat tactggggtc aagaattcgc agacaagaaa tttgctgtaa
     1681 tgcttgaagg atcttggtta ttgggacaat ttgataagaa cgaatggaat acattaaaag
     1741 aaaaaatagg aatgattcca atgtttccgg tacctaatac tctaaataca aacacttcta
     1801 cattaatggg agggtggctc ttaagtatac ctgaaacgtc acaaaataaa gaaatatctt
     1861 gggaacttct tactataatg ttggagccga atgttttgat tccgatgttg ttacaacagg
     1921 gctatcttcc aactcaaaaa agtataggag aaggacaata ctctgaggag ttgaaatcaa
     1981 caattcctta ttatgaagaa ctcatttctc taattgaatt agggaaagga agacccaata
     2041 ttcctgagta tcctatagtt gccgagcata taagaaatgc aattgaacaa gtatacaatg
     2101 gaatggatcc aaaa
//
LOCUS       DATAFW010000024         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_360547, whole genome shotgun sequence.
ACCESSION   DATAFW010000024 DATAFW010000000
VERSION     DATAFW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360547"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VHJ57_00475"
     CDS             <1..333
                     /locus_tag="VHJ57_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487041.1"
                     /translation="LYKREIDKLTSTNKEIDQSTPTKVNPEKDTKESKNPDKKGSIFQ
                     NGSNSDIKPIIFGLFALIGFIIIVYSIFMGVVGEPLASIADTVFIAALIGSFTLVSTI
                     VGLIFLKK"
     gene            544..864
                     /locus_tag="VHJ57_00480"
     CDS             544..864
                     /locus_tag="VHJ57_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487042.1"
                     /translation="MEDIIEHFAQRLEISSDMARQGISITSKFFLQNSEPVVATGLLS
                     MLPTSLTNMFSNDEKQEFKTSQKNISYDEIIKKISNECFNGDLQKAKKVYEEAINVIK
                     RQIW"
     gene            complement(1171..1551)
                     /locus_tag="VHJ57_00485"
     CDS             complement(1171..1551)
                     /locus_tag="VHJ57_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX2487043.1"
                     /translation="MKQPYADLLAFGKKTIELRKWNTKFRGEFLIHASKNIDIQACER
                     LDIDIDKLTLGAIIGSAFLYDFKEYSNQEEFNKDKQKHFSIVTKYFDGYKYGFLIRNA
                     RMFKKSIPYPGKLRFFEVDNSIKF"
BASE COUNT          751 a          213 c          229 g          670 t
ORIGIN      
        1 ctttataaaa gagaaataga taaactaact tctactaata aagaaatcga tcagtctacc
       61 cctacaaaag ttaatcctga aaaagacact aaagaatcaa aaaatccaga taaaaaagga
      121 tcgatatttc aaaatggtag taacagtgat attaaaccaa taatttttgg cctatttgct
      181 ttgattgggt tcataattat tgtatattca atatttatgg gagtcgtagg tgaaccatta
      241 gcatcaatag cagatacagt atttattgct gcattaattg gatcctttac tttagtcagt
      301 acaatagttg gtcttatatt cttaaagaaa tgaaaaattt ctaaaatcta gttattgtat
      361 ctatagttat ctatagtgat tattttggtt ttgacaaata tgatcgttaa cattttttga
      421 atatcttagt gaattctatg attattccaa tattagaaaa ataatggcat ggaaaaaaat
      481 tcagcatcat tatggataaa aatattacca attgattaaa tatttaatta tcatttattc
      541 gttatggaag atattattga acattttgcc caaagattgg aaatctcatc tgatatggca
      601 agacaaggaa tttcaataac atcaaaattt tttcttcaaa attcagaacc tgtagtagca
      661 accggtcttt tatcaatgct accaacaagt ttaacaaata tgttttcaaa cgatgaaaaa
      721 caagaattta agactagtca aaaaaatatc tcatacgatg aaattattaa aaaaatatct
      781 aatgaatgtt ttaatggtga tctacaaaaa gcaaaaaaag tatatgaaga agcaataaat
      841 gtgataaaga gacagatctg gtaaaaataa agacaagtaa tagttgatat gggtgacaat
      901 caagatatac atttttaaat aaatatatat agtatcagag aatgttatca atttccataa
      961 tcaaaacata actattaaac aaaaaaaata tagattatat agaatattat ataaattatt
     1021 tataaatcat taatttttta tattactata tgaagaaaac aaaaatataa caattattca
     1081 tgaattgatt attggtatat gattatcaga aaaaatatca attggctaaa tagaaatgaa
     1141 agattaattc ataaataata gtctttataa ttaaaatttt attgaattat ctacttcgaa
     1201 gaatcttaat ttgcctggat aaggaattga ttttttaaac attctagcat ttctaattaa
     1261 gaaaccgtat ttgtagccat caaaatattt agtaacaata gaaaagtgtt tttgtttatc
     1321 tttgttaaac tcttcctgat tgctgtattc tttaaaatca tataaaaatg cagagccaat
     1381 gatagcgcct aatgtaagct tgtcaatatc aatatccaac ctctcacaag cttgtatatc
     1441 aatatttttt gaagcatgaa ttagaaattc tcctcgaaat ttagtattcc attttcttaa
     1501 ttctattgtc tttttaccaa aagctagtaa gtctgcatat ggttgcttca aagataaaca
     1561 tttcatttca taaatatatg atataattaa aaaaatttga gattatattt attatgtttt
     1621 atattatata atactaagaa acaacccagt atttcttctt tcaaagaaat aatgatacat
     1681 aagttgtata aattattatt attataatta tttaggaata acaagaaaca aaattattaa
     1741 cagacaataa aaaattagtg gttgtaataa gatttttcta tcttatttac aaagttaaag
     1801 taattttgaa ctgattcatt atagtatttt tgtgcaattt ctattgattt gttaaaggtc
     1861 tct
//
LOCUS       DATAFW010000025         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3708718, whole genome shotgun sequence.
ACCESSION   DATAFW010000025 DATAFW010000000
VERSION     DATAFW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3708718"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2025
                     /locus_tag="VHJ57_00490"
     CDS             <1..>2025
                     /locus_tag="VHJ57_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487044.1"
                     /translation="TGPTDFWDIETVVPEWLDKGKYWHIMLRLQIKNKEIILKVVKDN
                     DLPFFSGRRNVATTSRENLKTNRTFKKKYHYVVCVPQWLVMNTEPSAFSRSELNVNDS
                     DLGLLMNAINKQLGLASNDITQHEKQRIKYSWKFSSLPIYLIKSLPIQIIYKEIFTKT
                     AKYIPTQKKHIIEETNINLAYLSNDELIESLLILARLKNIHSKSIEKINILLQFLKNE
                     YEKLGLGDGSKRYHNLHHSLEVAYVSLQMLPEELHEYHFSEEDYEYILIASLLHDYDR
                     YQYNYKTSIGLYRFEGPKVENTIRELLKIRIIDAYFSMNEIEFKNYFRQYQYHLLPPV
                     DYVTVHPEYVKPNKPLKSLIIEALIWRTDYPFTKKPQAQRNYYELIDKISHLIGSSEK
                     YILLSEVLSLADLSVTYMSSDPINAWYRVTSLYEELNLPRSEAISKTDQFFSEIMTIE
                     LFQELLNNRNFPSVFKQRWDLVYQFYHEGNPSTQINRTIENAQISYSKINLEIGVQTG
                     DILYHIALEHKDEYFIGISTDEEAVLRIKEKFANLMIQNVYCFLGDAKKLLPNLNRRS
                     IDNILFIFNNFPTYENPNGQDPELLLLFKGVSLSLKSHGTFQILTNISKNSDKEKEII
                     EIASKYGFQLLKSNNEHKIYLPQSYIPNEFFQNDSVSLLVFHLYINSTIVG"
BASE COUNT          782 a          278 c          289 g          676 t
ORIGIN      
        1 gacaggaccc acagattttt gggacataga aactgtggta cctgaatggc tagataaagg
       61 aaaatattgg catattatgc ttagactgca aattaaaaac aaagaaatca ttcttaaagt
      121 tgttaaagat aatgatcttc cttttttttc tggtagaaga aatgttgcaa caacttcaag
      181 agaaaactta aaaactaatc gcacgtttaa aaaaaaatat cattatgtcg tttgtgttcc
      241 acaatggcta gtaatgaata ctgagccgag tgctttttct agaagtgaac taaatgtaaa
      301 tgattctgat ttaggtttat taatgaatgc tattaataaa caactcggat tagcgtccaa
      361 tgacattaca caacatgaaa aacaaagaat taagtattct tggaaattta gttctttacc
      421 aatttactta ataaagagtt taccaatcca aattatttat aaggaaattt ttactaaaac
      481 agctaaatac atacctacac aaaaaaaaca tattattgaa gaaactaata ttaatttggc
      541 atatttaagt aatgatgaat taattgaatc attactaatt cttgcaagac ttaagaatat
      601 acattcgaaa agtatagaaa aaattaatat tcttttacaa tttcttaaaa acgaatatga
      661 aaaattaggg ttaggtgatg gttctaaaag atatcataac cttcaccata gtctagaggt
      721 agcatatgtt agcttgcaga tgttacctga agagttacat gaatatcatt tttcagaaga
      781 agattatgaa tatatcttga tagcaagctt attacatgat tatgatcgtt atcaatataa
      841 ttataaaacc agtataggat tatatcgatt tgaaggtcct aaagttgaaa atacaatcag
      901 agaactcttg aaaataagga ttatagatgc ttattttagt atgaatgaaa tagaattcaa
      961 aaattatttt cgtcaatatc aatatcattt attaccacca gttgattatg ttactgttca
     1021 tcctgagtat gtgaaaccta acaaacctct caaaagttta ataattgaag ccttgatctg
     1081 gagaacagat tacccattta caaaaaaacc tcaagctcaa agaaattact acgaattaat
     1141 tgataaaatt tctcatttaa ttggatcatc cgaaaaatac atattattat ctgaagttct
     1201 atccttagca gatttatcag taacatatat gagctctgac cctattaatg cttggtacag
     1261 agtaacaagt ctatacgaag aattaaacct tcctcgatct gaggctattt ctaaaactga
     1321 tcaatttttc tctgaaatca tgacaataga gttgtttcaa gaattattaa ataatcgtaa
     1381 ttttccttca gttttcaagc aacgatggga cttagtttat cagttttatc atgagggtaa
     1441 cccatcaacg caaataaaca ggacaataga aaatgctcaa atttcatatt caaaaattaa
     1501 cttagaaatt ggtgtccaaa ctggggatat tttgtaccat atagcattag agcataaaga
     1561 tgaatatttt ataggaataa gtactgatga agaggctgta ctccgtatta aagagaaatt
     1621 tgccaatttg atgatacaaa atgtatattg ttttttaggg gatgcaaaaa aactattacc
     1681 taatcttaac agaaggtcta ttgataatat tttatttata tttaataatt ttcctactta
     1741 tgaaaatcct aatggacaag acccagaatt attgttatta tttaaaggag tttcactatc
     1801 tcttaaatcc catggtacgt ttcaaattct tactaatata agtaaaaata gcgataagga
     1861 aaaagaaata atagaaattg catcaaagta tggctttcaa ctattgaaaa gtaataatga
     1921 acataaaata tatttgcctc aatcatatat cccaaacgaa ttttttcaaa atgattcagt
     1981 ttcattgtta gtatttcatc tttatattaa ttctactatt gtagg
//
LOCUS       DATAFW010000026         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5118314, whole genome shotgun sequence.
ACCESSION   DATAFW010000026 DATAFW010000000
VERSION     DATAFW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5118314"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VHJ57_00495"
     CDS             <1..204
                     /locus_tag="VHJ57_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2487045.1"
                     /translation="ALTTKKIAGAALDVFEVEPPTNKKLLQLSNIICTPHIGSQTKES
                     QELASNVIAEKIIQKLIERSNSG"
     gene            complement(214..307)
                     /locus_tag="VHJ57_00500"
     tRNA            complement(join(214..250,271..307))
                     /locus_tag="VHJ57_00500"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(271..273),aa:Ala,seq:tgc)
     gene            1129..2139
                     /locus_tag="VHJ57_00505"
     CDS             1129..2139
                     /locus_tag="VHJ57_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2487046.1"
                     /translation="MLSEISKRGVYPLHGYKLGIFRLPVNLTEESTINNIFNELKKTG
                     FKLDRYADRIYVTYTWKEQETATFLSKILSIPLDITIEIVTGEVIDLIYQIKPIEKFG
                     DTYRIKNYRQRADLNAKMVIDTILRNTSIGDKLISYYQKTEKLSLENAIDKLTQLTPL
                     AVNPITRNVTPPPPPAGASITSQEISSTSVGSTRSAPNIPDTPSAGTMTSGTSATSIS
                     LTGSGTDYSKVEGPIDTSFKSKRQKVGKFKGINVWGPVDPPGQLGIWGDSVCVDFDIC
                     IGDGACIEACPVNVYEWLETPGHPGSEKKPFMAREKDCIFCLACENVCPPQAIKIYEK
                     KS"
     gene            2188..>2288
                     /locus_tag="VHJ57_00510"
     CDS             2188..>2288
                     /locus_tag="VHJ57_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HEX2487047.1"
                     /translation="MEKLNLVATGGTFDYIHIGHIKLLSTAFDISHHV"
BASE COUNT          831 a          353 c          347 g          757 t
ORIGIN      
        1 gctctaacaa caaaaaaaat tgctggagct gcattggatg tttttgaagt agaaccacct
       61 actaataaaa aattgttgca attatcaaat atcatctgta ctccccatat aggctctcaa
      121 actaaagaat ctcaagaatt agcttcaaat gttattgcag agaaaattat tcaaaaatta
      181 atagaaagat ctaattcagg ataaataatt gtatggaccg ggtgggattt gaacccacga
      241 ccacctacgt ttcctctgcg tcaatggcat gcaaggcagg tattctacca ggctaaacta
      301 ccggcccttt aatgacaacc tgaatttctg ataatttaag tgtttaatta ctgtaagtta
      361 atcttatgat ttaaacatgg gttattttta tacaaatagg caagattgtt ataatatcaa
      421 acattcattt ttttaaataa cttcatttgt aagttatcac actaatttag tgatggattc
      481 aataaaacct tattatgtta gttaaaaaaa acctcaaaat acttaatatt ctaaacataa
      541 taatagaatg gtttgagaga gtataaatga ccaatttaat ataatatttt aattgaacaa
      601 agattaaatt tttttaatca taaattttga ttaaacattt aaccttctag taatatactt
      661 gttatgaaag ttgagtttat cagggttgat tttatatcta ttctaatttt atggacactt
      721 ataataaatt gctgactata agaaatagaa atatctaggg ttatagaaaa tttctattat
      781 tgttaaatta gattagatta tagctaaatt aattcttata aaatcaatta ctataatagt
      841 cctaactatt tttacaaatt gaagaattct gccaaatgaa gaaaggataa tctttaattt
      901 aaagacaaac tcctactttt gactgaattg aaatatctcg aaatatagag catcaaaaat
      961 ttaataacaa ttgtagctaa aaaaccatat tcagccaggt agatgtattt aaagaatatt
     1021 tcaagaaatt cgagagataa cttattagag gattaaaatc gaaacccttt taggtttttc
     1081 aatttaaata aacatatgtc tgttttctta aaggataggg tttggatcat gctttctgaa
     1141 atttctaaac gcggagtata tccccttcat ggttataaat tgggtatttt tcgattgcca
     1201 gtaaatttaa cagaggaatc aacaatcaac aatatcttca atgagttaaa gaaaacaggt
     1261 tttaagttag atagatatgc tgaccgcata tatgtaacct acacctggaa agaacaagaa
     1321 acagctacct tcctatctaa aatattatcc atacctcttg atataacaat agaaattgta
     1381 acaggagaag tgattgattt aatctatcaa attaaaccta ttgaaaaatt cggtgatacg
     1441 tatcgaatta aaaattatag acaaagagct gatctcaatg ctaaaatggt aattgatact
     1501 atattacgta atacttcaat tggagataag ctaatttctt attaccaaaa aacagagaaa
     1561 ttatcccttg agaatgctat tgataaactt acacaattaa ctcctttagc agttaatcca
     1621 ataactcgaa atgtaactcc tccacctcct ccagctggtg catcaattac ctctcaagaa
     1681 atctcttcta cctcagtagg ctcaacaaga tctgctccaa atattccaga tactccatct
     1741 gcaggcacta tgacatctgg aacttctgct acatctatct cgcttacagg aagcggaacc
     1801 gattattcca aagttgaggg ccctattgat acctctttta aatctaagcg acaaaaagtt
     1861 ggcaaattta aagggattaa tgtttggggc cctgttgatc ctcctggaca gttaggaata
     1921 tggggcgata gtgtctgtgt agattttgat atttgcatag gtgatggtgc atgtatagaa
     1981 gcttgtccag ttaatgtcta tgaatggtta gaaacccctg gtcatccagg atctgaaaaa
     2041 aaacctttta tggctagaga aaaagattgt atattttgtt tagcatgcga aaatgtgtgt
     2101 ccaccacagg ctatcaaaat ttacgaaaaa aagagctaat aatattacta tttttctttt
     2161 attgataaat tatttagata aagacgattg gaaaaattaa atcttgtcgc aacgggtgga
     2221 acttttgact atattcatat tggtcatata aaacttcttt caactgcttt tgatatcagc
     2281 caccatgt
//
LOCUS       DATAFW010000027         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3179877, whole genome shotgun sequence.
ACCESSION   DATAFW010000027 DATAFW010000000
VERSION     DATAFW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3179877"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VHJ57_00515"
     CDS             <1..335
                     /locus_tag="VHJ57_00515"
                     /inference="COORDINATES: protein motif:HMM:NF003084.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HEX2487048.1"
                     /translation="YKEGLDLGKRKVLNEAALKELVMPHEGEMFGRVIKLVGGDNVIV
                     KCNDGKVRTCRIRGKIKRRMWIRENDLVLLAPWDFQSDKADIIWRYIAAHADKLEQEG
                     HLKGLINN"
     gene            complement(430..1254)
                     /gene="hisF"
                     /locus_tag="VHJ57_00520"
     CDS             complement(430..1254)
                     /gene="hisF"
                     /locus_tag="VHJ57_00520"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX2487049.1"
                     /translation="MTLAKRIIPCLDVDNGRVVKGINFKEVKDAGDPVEFAKNYSQQG
                     ADELVFLDITASEQKRDIIKNVVRNVARVIDIPFTVGGGIKKLEDARDILLSGADKVS
                     INTSAIKNPELITELMSIFGKQCIVVAIDVKKNYYEIDPKETLKDNYEHKLKQNKPYW
                     FEVMIYGGKERTGIDAIKWARKVEKLGAGEVLLTSIDADGTEEGYDLELNKIICRKLN
                     IPVIASGGCGKPIHMLEVFQKTKVDAALAASIFHYKKSTIKRVKQFLKRNNIYVRI"
     gene            complement(1257..1976)
                     /locus_tag="VHJ57_00525"
     CDS             complement(1257..1976)
                     /locus_tag="VHJ57_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HEX2487050.1"
                     /translation="MNIIPAIDILEGKVVRLVKGDLQNKIVYSDNPIEIARKMEAQGV
                     NIIHVVDLDATLTTGWNNNQNNTEIIFKIIDSIKIPVQVAGGIRSIDAINRMFEKKAG
                     KIVIGTLAYRQPHMLQQLKDKVEKIVISIDQNKGIVMIDGWRQPSGFKVVEAIKLFMA
                     KGVKEFLLTSVDRDGTLNGPDLDTLSYASNINGAKIIASGGISSIEDIIRVKNIDCSA
                     VILGKSLYDGKIDVKKALTIV"
     gene            complement(1973..2593)
                     /gene="hisH"
                     /locus_tag="VHJ57_00530"
     CDS             complement(1973..2593)
                     /gene="hisH"
                     /locus_tag="VHJ57_00530"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX2487051.1"
                     /translation="MNDIAIFDYGAGNLYSLKCSLERNGATKVSVIKSLKNIHKHDGL
                     ILPGVGNFDPAIRSIQPHISDFKNLLEKNFPILGICLGMEMLFSNSEEGKLEGLKILN
                     GRVIMLPIKKVKIPHMGWNNLEITEKACKILHGLNEGCWVYFVHSYYVVSKDKKIIVA
                     NSQYGGTIPVVVEKNNLFGTQFHPEKSGINGAKIIKNFLTICGGKY"
     gene            complement(2596..>2791)
                     /locus_tag="VHJ57_00535"
     CDS             complement(2596..>2791)
                     /locus_tag="VHJ57_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX2487052.1"
                     /translation="REDLEHFIESLLQNLNACTHINIQYGENDHHKIEAAIKSFAIAF
                     RMASNIDYQRNDIPSTKGVM"
BASE COUNT          859 a          487 c          355 g         1090 t
ORIGIN      
        1 tatataagga aggattagat ttaggcaaaa gaaaagtttt gaatgaggcg gcattaaaag
       61 agcttgttat gcctcatgaa ggagaaatgt ttggaagagt tataaaactc gtaggaggcg
      121 ataatgttat tgtcaaatgt aatgatggta aagtacgtac atgccgtatt cgaggtaaga
      181 taaaaagacg aatgtggata agagaaaatg atctagtact tcttgcacct tgggattttc
      241 aatctgacaa ggcagatata atttggagat atattgcggc tcatgctgat aagttagaac
      301 aagaaggaca tttaaaaggt ttaataaata attaataaat gcgagttaat ttaattttta
      361 attaatcttc tttaatatta gatctgataa tataaataga taatttacaa taacaaaatc
      421 gattttatat catattctta cgtatatatt gtttcttttt aaaaattgtt ttactctttt
      481 aatagtagac ttcttataat gaaatataga tgctgctaaa gctgcatcta cctttgtttt
      541 ttggaaaact tccaacatat gtattggttt gccacatcct ccagaggcta tgactggtat
      601 atttaatttt ctacaaataa ttttgttcaa ttctaaatca tatccttctt cagttccatc
      661 tgcatcaata cttgttaaaa gaacctctcc tgcccctagt ttttctacct ttctagccca
      721 ttttatggca tctatgcctg ttctttcttt tcctccatat atcataactt caaaccaata
      781 aggtttattt tgtttcagtt tatgctcata attatctttt agagtttcct taggatcaat
      841 ctcataataa ttttttttaa catctattgc tactactata cattgttttc caaaaataga
      901 cattaattct gtgattaatt ctggattttt tatagcagat gtatttattg aaactttatc
      961 agctccactt aataaaatat ctctagcatc ttctaatttt ttgatacctc cacctacagt
     1021 aaagggtatg tctataactc tagcaacatt tcgaactaca tttttaataa tgtctcgttt
     1081 ctgttctgaa gcagttatat ctagaaaaac aagctcatct gcaccttgtt gactgtaatt
     1141 ttttgcaaat tcaactggat ctcctgcatc ttttacttct ttaaaattaa tacctttaac
     1201 tactctacca ttgtctacat cgagacaagg aataattctt tttgctaaag tcatttttaa
     1261 actattgtta gtgctttttt tacatctatc tttccatcat agagagattt accaagtata
     1321 actgccgaac aatcaatatt tttgactcgt attatatctt caatactaga tattccacca
     1381 ctagcaatta tttttgctcc atttatgttt gaagcatagg agagtgtatc taaatctggg
     1441 ccgttcaaag ttccatctct atccactgag gtgagtaaaa attctttaac tcccttggcc
     1501 ataaataatt ttatagcttc aactacctta aatccagatg gttgcctcca tccatcaatc
     1561 atgacaatac ctttattttg atctattgaa attactattt tctctacttt atcctttaat
     1621 tgttgtaaca tgtgaggttg tctgtatgcc aatgttccta taacaatttt tcctgctttt
     1681 ttttcaaaca tcctgttaat agcatcaata gatcttattc ctccagctac ctgcacagga
     1741 atttttattg aatctattat tttaaaaata atttctgtgt tattttgatt attattccat
     1801 ccagtagtca aagtagcatc aagatctact acatgaatta tattcacacc ttgggcttcc
     1861 atttttcttg caatttctat aggattatca ctataaacaa ttttattttg aagatctcct
     1921 ttaactaatc ttactacctt tccttctagg atatcaattg caggtattat attcaatatt
     1981 taccaccaca tatagtcaag aaattcttta ttattttcgc tccgtttata cctgattttt
     2041 caggatgaaa ttgcgttcca aaaagattat ttttctctac aactactggt atagtacctc
     2101 cgtattgaga attagcaact attatttttt tatcttttga cacaacatag tatgaatgaa
     2161 caaaatagac ccaacatccc tcatttaatc catgtaggat tttacatgct ttttctgtta
     2221 tttctaaatt attccatccc atatgtggga tttttacctt ttttattgga agcataatta
     2281 ctcttccatt taaaattttc aaaccttcta actttccctc ttcgctatta ctaaataaca
     2341 tttccattcc tagacaaatg ccaagtatag gaaaattttt ttcaagcaga ttcttaaaat
     2401 cgcttatatg tggttgtatt gatcgaatag cagggtcgaa gttcccaact cctggaagaa
     2461 ttaatccatc atgcttatgg atgtttttta aagatttaat tacagaaact tttgttgctc
     2521 catttctttc caaagagcat tttaaactat aaagattacc tgcaccataa tcaaaaattg
     2581 caatatcatt caatctcaca ttactccttt tgtacttgga atatcgtttc tttgataatc
     2641 tatatttgaa gccattctaa atgctattgc aaatgacttt attgctgcct ctattttatg
     2701 gtggtcattt tcgccatatt gtatattaat atgagtacat gcattaagat tttgtaataa
     2761 actttctata aaatgttcca aatcttctct t
//
LOCUS       DATAFW010000028         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5294950, whole genome shotgun sequence.
ACCESSION   DATAFW010000028 DATAFW010000000
VERSION     DATAFW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5294950"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VHJ57_00540"
     CDS             complement(<1..429)
                     /locus_tag="VHJ57_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487053.1"
                     /translation="MKIESKNFKPFDNISIPYEGYLGEGFFIIKGKNSMGKTSIIESI
                     LWGLLGEHLMAEKDRKSIIKTGESKCEVAITFEISDILYRVIRTIEVIRKRKSFEDPQ
                     NDLVKSSALLFKKEKNENKFIQLCRGPNIVNQEIESLLGVY"
     gene            complement(446..1723)
                     /locus_tag="VHJ57_00545"
     CDS             complement(446..1723)
                     /locus_tag="VHJ57_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2487054.1"
                     /translation="MRIIHISDTHLGKRPKRTRASIINQEIKPIEDDFYNSWRRFIEE
                     IINLDKDEKPDIIIHSGDFFDTPSGTDPSPPPEFARKVAAETFKKLHENNIPIVIIDG
                     NHGRYMQYRISPITEYTVLFDNVYLFTYFDIRDSIRNQQPLFKDFPTLNLRIHAHPSL
                     ESNDLPQLLLKYEDWVHIQNNNINPGMINVFLAHGMIENHTLNSNFLMGDYDYIGLGD
                     NHKMQQISDNAWYAGSIELWSFNEETYQKGYLVIDIDDSSSSSSKSKIKVIQKLLPQQ
                     RKIVSNHIEIYPDDNNSKIIDRIKKVFEKNELNTPYDYQTSARVKITIQGNRSYGSFY
                     NIHEISSYLNKITLNSNEYNIVEFILETPKYYDDTVIKQIKQDDTFIEYLIEDPEKEF
                     KKYITSTRKEDLKKNNLDANMLAKLFFESLKNQ"
     gene            complement(1787..3682)
                     /locus_tag="VHJ57_00550"
     CDS             complement(1787..3682)
                     /locus_tag="VHJ57_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2487055.1"
                     /translation="MSSNNINNNDNNNKIKNNDSYLNCDIKLNQLLSNTEPIGTIGSP
                     SSTSEISLNILQSAVTKKLIGELVIFRYIQESKSNYSLGQITEIELKNVLLEDPTIKS
                     VARQRGLVNPISGIQDTHVGKLLCSAVFSNNDEENFFDTSLLGTVPATGTYIHVTNDN
                     ILNSLLEKHLDKIFYLGYVYGSQTKLPMWFKHFGDGIEGAGEAYHLGIFGTTGSGKST
                     LAKMILMAYAKYPEMGLFVFDPVGEFSKDAIFDNASGLNYQSKNNGFNLNIKEILNSL
                     GKKFEIINIRNLILDRWELFEEILYESSFFPNLSVKGENKRDAVTILINELKNRLHIS
                     LTNLIELESFQQVLNILRNPNIQLLIYSTAEPRARLNIVLSEIDENYLYERFWKPICQ
                     LFHYRPGAQTVDSLLKRFTSDEKPLVIVDLSLQSAHHLKLDYWNETIQALILKRILYG
                     LINLGERSWQQNTSLNTLVILDEAHRFARREKYENSDLENLRLTLLDAVRTTRKYGLG
                     WMFISTSISSVHKDILQQLRIMFCGFGLSIGSDLVTLRELVSDHNALKLYQSFTDPAT
                     SFSTKSRKYSFMSQGPVSPLSFAGTPLFLNVFNSSETFVEENKLLSYTKKSIPNSLTY
                     TQDNYDF"
     gene            complement(3688..>4698)
                     /locus_tag="VHJ57_00555"
     CDS             complement(3688..>4698)
                     /locus_tag="VHJ57_00555"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEX2487056.1"
                     /translation="DRSEILGKDTEINSHKILNSCGIDGAFAIERLLGTDLIAIAAVG
                     VEGLMSPYQKGKWDSPNHNVFINPERHDPENMIAARGLMIEMEINLAANAPYDLIFLD
                     GSLTTGLIHMYKAIDFIEKRKSVTANQIRDNYKNFLISYKKILESKDNNKRQWIGIPK
                     YTSRNDIGDIYDWPTDYDDRAILTLILNEGEYTIPKPFALKENWHKKLPTIDNGKDDY
                     ELEDLMEGVIDEIKGLHVIYYKPYKWSPAIRIEISSSVANDSNRFYALLQSIKSQCSI
                     PSIMEPYPLYLADRMVKHIAHAIPAYKQVLTRNMTEFEKNDESISDIIFIMHSYRTES
                     GM"
BASE COUNT         1512 a          712 c          613 g         1861 t
ORIGIN      
        1 gtatactccc aaaagtgatt caatttcttg attaacgata tttggccctc tacatagctg
       61 aatgaattta ttttcatttt tttctttttt aaatagcaat gcagaagatt ttactaaatc
      121 attttgagga tcctcaaatg actttctttt tctaatgacc tcaattgttc ttattactct
      181 atatagaatg tcacttattt caaatgttat agctacttca catttagatt ctccagtttt
      241 aattatcgat tttctatctt tttctgccat caaatgttca ccaagtagac cccatagaat
      301 agattctatt atacttgttt tacccattga attttttcct ttaataataa agaatccttc
      361 tccaagatac ccttcatatg gtatggaaat attatcaaat ggtttgaaat tttttgattc
      421 tattttcaaa attttcataa tatcactact gattctttaa tgattcgaag aaaagttttg
      481 caagcatgtt agcatctaaa ttattttttt ttagatcttc ttttctagta gatgtaatat
      541 attttttaaa ttctttctct ggatcttcta ttaaatattc tataaatgta tcatcttgtt
      601 taatttgttt aataacagta tcatcataat attttggtgt ttctagaata aattctacta
      661 tgttatactc attgctattt aatgttattt tatttaaata agatgatatt tcatggatat
      721 tataaaatga accatagctt ctattacctt gtatagttat tttaactcta gcagaggttt
      781 gataatcata aggtgtattg agttcatttt tctcaaatac ctttttaata cgatcgataa
      841 ttttagaatt attatcatct ggataaattt ctatatgatt acttactatt tttctctgtt
      901 gaggtaaaag tttttgaatt actttaattt tagatttaga agaagaagaa gaagaatcat
      961 caatatctat aaccaaataa cctttttgat aagtttcttc attaaaactc cacaattcta
     1021 tcgagccagc ataccatgca ttatcactga tttgttgcat cttatgatta tctccaagtc
     1081 caatataatc ataatctccc atcaaaaaat tgctgtttag tgtatgattt tctatcattc
     1141 catgagctaa aaatacgttt atcataccag gattaatatt attgttttgt atgtggaccc
     1201 aatcttcata ttttaataat aattgtggta agtcattgga ttctaatgat ggatgtgcgt
     1261 gaattcttaa attcaaagtt gggaagtctt tgaataaagg ttgttgattt cttatactat
     1321 ctcttatatc aaaatacgta aaaagatata cattatcaaa caaaactgtg tattcagtta
     1381 ttggcgaaat tctatattgc atataacgac catgatttcc atcaattata acaatgggaa
     1441 tattattttc atgtaatttt ttgaatgtct ctgcagctac ttttcttgca aattctggtg
     1501 gtggtgaagg atcagttcct gatggagtgt caaaaaaatc tccgctatga attatgatat
     1561 caggtttttc atctttatca agatttatta tttcttctat aaatcttctc caagagttat
     1621 aaaaatcgtc ttctattggt tttatctctt gattaataat tgaagctctg gttctttttg
     1681 gcctctttcc aagatgggta tcagaaatat gaattattct catttttagc aaaatttaat
     1741 tttaaataat aaaatattaa aattacaaat gaaaataata taacaattaa aaatcataat
     1801 tatcctgagt ataagttaaa ctatttggta tactcttttt tgtatatgaa agtaatttat
     1861 tttcttctac aaatgtctct gatgaattaa agacatttaa aaaaagtgga gttccagcaa
     1921 aagaaagagg agatactggt ccttggctca taaaagaata ttttctggat ttagtactaa
     1981 atgaagttgc aggatcagta aatgattgat atagtttaag tgcattatga tcagatacta
     2041 attctcttag agtaactaaa tcacttccta ttgataaacc aaaaccacaa aacattattc
     2101 ttaattgttg aagaatatcc ttatgtacac ttgaaattga ggtacttata aacatccatc
     2161 ctaacccata ttttctagtt gttctcactg cgtccaagag agtaagtctt agattttcaa
     2221 ggtcggagtt ttcatatttt tctcttcttg caaatctatg tgcctcatca agtataacta
     2281 aagtatttag tgaagtattt tgttgccaac ttctttctcc taaatttatc aaaccgtaca
     2341 aaattctttt tagaattaat gcttgtatag tttcattcca ataatccagt tttagatgat
     2401 gagcactttg taatgataga tctactataa ctaaaggttt ttcatcactt gtaaatcttt
     2461 tgagtagaga gtctacagtt tgtgctcccg gtctataatg aaatagttga caaatcggtt
     2521 tccaaaatct ttcatataaa taattttcat cgatttctga aagaactata tttagtcttg
     2581 ctcttggttc agcagtagaa tatattaata gttgaatatt aggattacgc aagatattaa
     2641 gtacttgttg gaatgattcc aattcaataa gattggttag acttatgtgt aacctatttt
     2701 ttagttcatt tattaaaata gtaactgcat cgcgtttatt ttctccttta acagacagat
     2761 ttgggaaaaa tgaagattca tataagattt cttcgaataa ttcccatcta tctaagatta
     2821 aatttcttat atttattatt tcaaatttct ttcctaatga atttaatatt tctttaatat
     2881 ttaaattaaa accattattt tttgattgat aattcaatcc actagcatta tcaaatattg
     2941 catctttaga aaattcacct actggatcaa atacaaaaag acccatttca ggatacttgg
     3001 catatgccat tagaatcatc tttgctaaag ttgacttacc agaacctgtt gtaccaaata
     3061 tacctaaatg atatgcttcc cctgcacctt caattccatc tccaaaatgt ttaaaccaca
     3121 tcggtaattt agtttgtgat ccatatacat atcctaaata aaagattttg tctaaatgtt
     3181 tttcgagtaa tgaatttagt atattatcat ttgttacatg tatatatgtt cctgtagcag
     3241 gtacagtccc taataaactg gtatcaaaaa aattctcttc atcgttattg ctaaagactg
     3301 cactacataa cagttttcct acgtgcgtat cttgaattcc acttatagga tttactaaac
     3361 ctctttgtct agcaacagat tttatagtag gatcttctaa tagtacattt ttgagttcta
     3421 tctctgtaat ttgtcctaaa gaataattgg atttagattc ttgtatatat ctaaaaatta
     3481 caagctctcc tattaatttt ttagttacag cactttgtaa tatatttaaa cttatttcac
     3541 tggtcgatga gggagatccg atagtaccta ttggttcagt attagataaa agttgattta
     3601 attttatatc acaattcaaa tatgaatcat tatttttaat tttattatta ttatcgttat
     3661 tgtttatatt atttgagctc atatctatca cattccactt tctgttctat atgaatgcat
     3721 aataaaaatg atatcagata tactttcatc atttttttca aattcggtca tatttcttgt
     3781 taatacctgt ttgtatgctg gaatagcatg tgcaatatgt ttaaccatgc gatcggcgag
     3841 ataaagagga taaggctcca taatagaggg aattgaacat tgacttttga ttgattgaag
     3901 taatgcataa aatctattac tatcatttgc tactgaagat gaaatttcaa ttctaatagc
     3961 aggggaccat ttgtaaggct tataatagat aacatgtaat cctttaatct catcaataac
     4021 tccctccatt aaatcttcca attcataatc atctttacca ttatcaatag tagggagttt
     4081 cttatgccaa ttttctttta atgcaaatgg ttttggaata gtatattctc cttcatttaa
     4141 aattaaagtt agaatagctc tatcatcata atctgtaggc caatcataaa tatctccaat
     4201 atcatttctg gaagtatatt ttggaattcc tatccattgt cttttattat tatcttttga
     4261 ttctaaaatt tttttataag atattaagaa attcttatag ttatcacgta tttgatttgc
     4321 tgtaacgctt tttcgttttt cgataaaatc tatagcttta tacatatgaa taagtcctgt
     4381 agtaagagat ccgtctaaaa agattaaatc gtatggtgca tttgctgcaa ggtttatttc
     4441 catttctatc attaatcctc tagctgcaat catattttca gggtcatgcc gttcaggatt
     4501 tatgaagaca ttatgattag gagaatccca ttttcctttt tgataaggag acattaaacc
     4561 ttctacccca acagcggcta tagctatgag atctgttcct aagagacgtt caattgcaaa
     4621 cgctccatct ataccacaag aatttaagat cttgtgagag tttatttcag tatcttttcc
     4681 taaaatctcg gatctatc
//
LOCUS       DATAFW010000029        13611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1242606, whole genome shotgun sequence.
ACCESSION   DATAFW010000029 DATAFW010000000
VERSION     DATAFW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242606"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3165
                     /locus_tag="VHJ57_00560"
     CDS             <1..3165
                     /locus_tag="VHJ57_00560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HEX2487057.1"
                     /translation="VTVRPSIILETGIRSEDDLTHKLVDIIRVNQRLKESKEAGTPPL
                     IVQDLVDLLQYHVTTYFDNEVSGIPQAHHRSGRPLKTLTQRLKGKEGRFRGSLSGKRV
                     DFSSRTVISPDPNIAISEVGIPYEVAKKLTIPETVSNWNIDKLKKLVFTGPNNYPGAN
                     YIIRPDGVKIRLDYVNDRQMVADSIVPGYIIERHLSDSDIVIFNRQPSLHRMSIMAHY
                     VRVLPYRTFRLHPAVCPPYNADFDGDEMNLHVPQSEEARSEAALLMSVQDQLISPRYG
                     GPIIGGIRDFITGAYLLTSDESFLSKEEFFNLALLGGYAGVLPEPKGEKDGKKLYTGK
                     QLFSLFLPKDFNFIITSKWNKSIKGEGKDVVIKNGELISGVIDKASIGAEEPDSVLHR
                     IAKDYGNDVAQQFLNSILIMLKTFITHRGFTYGYSDLWLSEDTHKEITEVITKAYDKI
                     GELIQQYKEGTLPLTRGLSPEEALELYLVNELSRARDRAGRIADRSFPNNNAGVIMAS
                     TGARGSTLNIGQMTAVLGQQSIRGKRIHKGYHNRSLPHFKINDTNPDAKGFVKSNYRD
                     GLTPLEFFFHAMGGREGLVDTAVRTQQSGYMQRRLINALEHLKLEYDSTVRDPHGNII
                     QYLYGEDGIDPAKSDHGEAVNISRIIESESVVDEGTRANEEEIRHILNQNISNFNLKL
                     KNNIETILTQNNLSKQGIEKVIKKIIDLTEKAMVEPGEAVGVVTAQSIGEPGTQMTLR
                     TFHYAGVKERNVTLGLPRLIELVDARKKPITPTMDIYLDEEHRISREKALTVAKEIIH
                     TKVIDVVEKTDIDYSGIIKLTFSENKLNDRGCILTDIFEILKGSKKKYDTTLDENEKS
                     IVLKMNEELDAQTLLLLKNKLLNTKIKGVPDIERVTIVKQNEEWVIQTAGSNLSKIIY
                     VDGIDLSRVTTNNVYEIWQTLGIEAARTALVREVTNTLEEQGLEVDIRHIMLVADLMT
                     SKGYLQQIGRHGIAGTKTSVLARAAFEITVPTIAKASMEGQTEALKGVTENVIVGATV
                     PVGTGMVDLYMKVNEQNEKSGENN"
     gene            3140..3439
                     /locus_tag="VHJ57_00565"
     CDS             3140..3439
                     /locus_tag="VHJ57_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEX2487058.1"
                     /translation="MKNLEKIIKDAKSSNKFKEGLKEVRSNIKGSKLIIVSNSLLNSD
                     KQFIEKSCKELKVPLYYYGDNSIKLGRLFNKPYRISSVALKSVEDDDVTALLATQ"
     gene            3518..3829
                     /locus_tag="VHJ57_00570"
     CDS             3518..3829
                     /locus_tag="VHJ57_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487059.1"
                     /translation="MDYQYNTDEENYLSTKKLFVILGLSVFGVFIVLALFVFPINNLI
                     RETVSEQAIIISKSENICVVETLDHPRQIDNCVYAVGDLVYVTFDAGTQRIKEHKLLH
                     E"
     gene            3995..4474
                     /locus_tag="VHJ57_00575"
     CDS             3995..4474
                     /locus_tag="VHJ57_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HEX2487060.1"
                     /translation="MTEKIKLTSDELRLISLFQSITSATARDCIVDEKMDRVIFVVNK
                     GQMGLAIGKGGTTIKQLQNVIPRRIELVEYSDNEDEFLKNILNSNMINEIKINKRIDG
                     TKQAIVVVDSKKKGVVVGKEGRNAEKARLLAKRYFHISNVLILSPEQTTHDNNGVNV"
     gene            4475..4912
                     /locus_tag="VHJ57_00580"
     CDS             4475..4912
                     /locus_tag="VHJ57_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX2487061.1"
                     /translation="MAKSPLGLFAGRVLQSKRKRSRWANKYYKRRVLLLDKKTNPMEG
                     APQARGIVLEKVGIESKQPNSAVRKCVRIQLIKNGKSITAFLPRDGALNFVDEHDEVI
                     VEGIGGSMGGAMGDIPGVRWQVFKVNGVSLKELVYGRKEKPRR"
     gene            4915..5523
                     /locus_tag="VHJ57_00585"
     CDS             4915..5523
                     /locus_tag="VHJ57_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX2487062.1"
                     /translation="MSGKENIKILLFNKWDTANIEINDPGLTRVISLNPSMVIPVSFG
                     RHEHQRLKKAEVNLIERLSNKLMRFGKKYAKNTGRMGGKKAKSLNVVKTAFEIIHLET
                     GKNPVELLVRAIENSSPNEDTTRIVYGGVVYHVSVDVSPLRRLDLALRFIAEGVREST
                     YSAPQMIEEALAKEILLASNNDMSSHAIKKKNEQERIAMASR"
     gene            5628..6764
                     /locus_tag="VHJ57_00590"
     CDS             5628..6764
                     /locus_tag="VHJ57_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487063.1"
                     /translation="MSSKDDFVFSKGALMSTKAGKEILKQAILRERGYKQYNKYKLKY
                     ETEFEGFTKRFLLSLHRRIISDTSPNSTVAQFAEEVGSHDMELDSSKVEDIKTRLSRP
                     EILADRVQRILDSNFVKMTFPVFNALFDGSVSYLKEDISNELRTSIIDGHIIAIDLSE
                     PMDRIMDKDEDLEYLDDYRLMNPYILDIAREKISQGGDSVLKAFEDGFKDARIGQLID
                     HKLKMKPESITDELMIGCYKKYRSIMGTAGRNMALNQTPLSEIYHLGMSKASESVGCG
                     NEMQDAINEGSIKIPSWPLYYSLIANDVRKGFELTLKKSEIYLDEAKLALEFLPEEFE
                     LKPFLIFLFLTVSHYNQYWYHDITKRDLFKYFERTIHPLTRKFI"
     gene            6787..8028
                     /locus_tag="VHJ57_00595"
     CDS             6787..8028
                     /locus_tag="VHJ57_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487064.1"
                     /translation="MYWAETYRPKNFEHIIGNEEIRLNILKWLVKWLPGTKPLLLIGP
                     PGVGKTTIVKILSSLLNYDLIELNASDTRSGTQLEKLITPLYNNTSIFGKKILMFFDE
                     VDGIYGREDSGGLEILINIVKAANFPIIFVANSNNIKLKNLSKLCKIFKFQHIPANLL
                     MMFLNYVLFKQGYSLTTEDKKWIVEKSNGDVRTMLNLVQSKCTGYDKFVHETFKVDIS
                     EGLNEFFHSKQKGDAKKILLTLDGKYPDPRYGLSSEERRKDIVNSLFTSFVSSNLDID
                     DLSLLLDRLSQIDLHVARINENRNWNLLRYMNNVIIQLLYENIQNKMISYNQYGISWP
                     VMGQVFSRGVPLRSLISKLSLSFHVSTSTFGMIFFPYILQIIHESKIDIHDLVSNLML
                     DEKFVEIIQKEILRSKRYERT"
     gene            8015..8395
                     /locus_tag="VHJ57_00600"
     CDS             8015..8395
                     /locus_tag="VHJ57_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487065.1"
                     /translation="MSEPDWIKLLVKFKGRCQSCENSISPGEYALWSKSSKQIKHLDC
                     KINDNKKTKKEKVEKYSELQLQCFLCKTVENIPISLLKEYLYHETQENSIFLCRSCLE
                     NHSSYLIYHEIMYEKVKKIVKLKF"
     gene            8415..8627
                     /locus_tag="VHJ57_00605"
     CDS             8415..8627
                     /locus_tag="VHJ57_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487066.1"
                     /translation="MSSEYERTIAKLLFEKKQKLDLLLENPQENKYQIQLLSTEIETL
                     QPIFENYNLGMNVFRRAQGGRSHLRE"
     gene            8756..10297
                     /locus_tag="VHJ57_00610"
     CDS             8756..10297
                     /locus_tag="VHJ57_00610"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX2487067.1"
                     /translation="MHEDKLKKLYELKNLAESGGGKERIDAQHSKGKLTARERIDLLL
                     DEGSFIELDKYVTHRSDDPETQKFYGDGVITGFGSIKGRKIFVYAYDFTVLGGSLGEM
                     ASKKIVKIMDRAIKVGAPLIGIIDSGGARIQEGVMSLDGYGDIFYRNCTASGIIPQIT
                     ASIGPCAGGAVYSPAMTDFVIMVENLGQMFVTGPEVVKEVLSEDVSFEELGGTSTHAT
                     KSGVAHMVAANEYECMEKIKKLISFLPQNNLQEPPSLETNDDPNRLDPSLIDMLPENP
                     YQPYDIKSIITSIIDDKEFFEIHETFASNIVVGLARMGGKTVGIVSNQPMYLAGVLDI
                     DSSNKAARFIRFCDSFNIPIITLVDTPGYLPGTDQEQNGIIRHGSKLLYAYCEASVPK
                     ITCIIGKAYGGAYIAMGSKNLKTDINFAWPSAEIAVLGPEAAVTIIHRRELKSSPDAK
                     TIKKKLAEEFRKKFANPYIAAEKGIIDQVIDPMETRTMIIGALDSLSGKREIGPWKKH
                     GNINL"
     gene            10310..11812
                     /locus_tag="VHJ57_00615"
     CDS             10310..11812
                     /locus_tag="VHJ57_00615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX2487068.1"
                     /translation="MPKISSILIANRGEIALRVIRACKELGIKSISVYSDDDNRSIHV
                     KRADEAYRLGPSQASESYLRIDKILEIAKSSGADAIHPGYGFLSENEVFSDECKKNGI
                     IFIGPTARAMELAGDKMKCKEIMKKAEVPIAPGSDGVVDDIELAVEIAKEARYPVMLK
                     SAFGGGGRGIRLVNNEKELRQEFELASNESQAAFGKSALFIEKYLTNIRHIEFQLIRD
                     SSGNGRHLFERECSIQRRHQKLIEMSPSPVVNKERREKVGEIAVRASTALDYLNAGTA
                     EFLADAEGNFYFIEINARLQVEHPITEIVTGKDLVKLQIHVAQGEELPFKQEDLELKG
                     SAIECRINSEDPFYDFAPSVGYVPNCNIPAGPGVRVDTYLYPGCNVSGYYDSLIAKLI
                     TYGGHFNEARIRMKNALSEFTIEGINTTIPIHKTIIEESNFINGNISTDYIEKYNIIE
                     KMKHKRTQEYKEKAYAAISGILLQSEFVKKSYLAHRIDRGQKSMDAGGFF"
     gene            11812..12324
                     /locus_tag="VHJ57_00620"
     CDS             11812..12324
                     /locus_tag="VHJ57_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX2487069.1"
                     /translation="MEFQIEGNANKINAELVYNKGSGDLLFVIDDQQYNLKLLKSTAN
                     EFEFILDNTYHYVKILPSTETKYKIYIDGIEVEIKKHSKITEIIEKTLKAKGTAYQAN
                     SVASQIPGRVVKVSVSNGDKVKEGEPIMVLESMKMQIAIKSHKEGIIKEIKVKEGSTV
                     SRNEIVAIIE"
     gene            complement(12847..>13611)
                     /locus_tag="VHJ57_00625"
     CDS             complement(12847..>13611)
                     /locus_tag="VHJ57_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2487070.1"
                     /translation="HLTEKTALVSIMLANNEIGVIQPIKEFSEIVHNFDKRIIFHSDA
                     VQAVGKIPVNVKELDLDSLSLSSHKINGPKGVGALYVREKVNFEPLIQGGGQELTFRS
                     GTENVPGIVGFGKASEISMLNLKENFHYLYYLRDYFINRINEEISGSMLNGSLESRLP
                     NNVNFTFLGINGEDLLIKLDEDGILASTGSACSANLQKESHVLKAIGLNHEEISGSIR
                     FTLGIQNTKKEIDKTIRSLSRRIKELRKFSPTQYRD"
BASE COUNT         4929 a         1939 c         2500 g         4243 t
ORIGIN      
        1 gtcactgtta ggccctctat tattcttgaa acagggattc gttctgaaga tgatttaact
       61 cataaacttg tagatataat ccgtgtaaat caacgactaa aagaaagtaa agaggcagga
      121 actccaccat taattgtcca agatttggta gatttattgc agtatcatgt tacgacttat
      181 tttgataatg aagtgtcggg tattccacag gctcatcatc gttcgggaag gccattaaaa
      241 actctaactc aaagattaaa aggtaaagaa ggtagattta ggggttcttt atccggaaaa
      301 cgagttgact tttcaagtcg aactgttatc tcaccagacc caaatattgc tatttcagag
      361 gtaggtatac cctatgaagt tgcaaaaaaa ttgaccattc ctgaaacagt gtcaaattgg
      421 aatatagata aactaaaaaa attagttttt accggaccaa acaactatcc gggggctaat
      481 tatattatta gaccagacgg tgtcaaaata agacttgatt atgtaaatga cagacaaatg
      541 gttgctgatt ccatcgtacc tggctatatt attgaacgac atttatcgga ttctgatata
      601 gttattttca atagacaacc atcattacat agaatgtcaa ttatggcaca ttatgtcaga
      661 gttctccctt atagaacctt tagacttcat ccggctgtat gcccacccta taatgcagat
      721 tttgatggtg acgaaatgaa tcttcatgtt ccacaaagtg aagaggcaag atcggaagct
      781 gctttattaa tgagtgtgca agatcagtta atatctccaa ggtatggagg acctataatt
      841 gggggtattc gtgacttcat tactggagct tatcttttaa ccagcgacga aagctttctc
      901 tctaaagagg aattctttaa tttagcatta ttaggaggct atgcaggagt gcttcccgaa
      961 cctaaaggag agaaagatgg taaaaaatta tatactggta aacaactttt ttcgttattt
     1021 cttccaaaag acttcaattt tattattact tcaaaatgga ataagtcgat taagggagaa
     1081 ggaaaagatg ttgtaattaa aaatggagaa ttgattagtg gtgtcataga taaagcatca
     1141 atcggtgctg aagaacctga tagtgtttta caccgaatag caaaggatta tggaaatgat
     1201 gtcgcacagc aattccttaa ttctatttta ataatgttaa aaacctttat tactcataga
     1261 ggtttcacat atggttattc agacttatgg ttatcagaag atacacataa agaaattact
     1321 gaagtaatta cgaaagcata cgataagatc ggtgaattaa ttcaacaata caaagaaggt
     1381 acgttacctt tgaccagagg attatctcca gaagaggcgt tagaattata ccttgtgaat
     1441 gaactttcca gagctagaga tagagctggc agaattgctg acagatcatt tccgaataat
     1501 aatgcaggag tcatcatggc ttctaccggt gcacgaggtt ccacattaaa tattggacaa
     1561 atgacagcag ttttaggtca acaatccatt cggggaaaaa ggattcataa aggttatcat
     1621 aatagatcat taccacactt caaaataaat gatacaaatc ctgatgcaaa aggttttgtt
     1681 aaatcaaatt accgtgatgg gcttactcca cttgaattct ttttccatgc aatgggagga
     1741 agagaaggct tagtcgacac tgctgtcaga acccagcaat caggatacat gcaaagaaga
     1801 ttgatcaatg ctcttgaaca tttgaaactt gaatatgata gtacggttag agatcctcat
     1861 ggtaatatta ttcaatatct ttacggagag gatggaattg accctgcaaa aagtgaccat
     1921 ggtgaagcag ttaatatttc aagaataatt gagagtgaat ccgttgttga cgagggaaca
     1981 agagcaaatg aagaagaaat tagacatata ttaaatcaga atatttctaa ttttaatcta
     2041 aaattaaaaa ataatattga aactatatta acacaaaaca atcttagcaa acaaggaata
     2101 gaaaaggtta ttaaaaaaat tattgatttg acagaaaaag ccatggtaga gccaggagag
     2161 gcagtaggag tagttactgc tcaatctatt ggggaacctg gtacacagat gacattgaga
     2221 acatttcatt acgcaggagt taaagaacgg aatgtaacac taggtcttcc ccgtttaatt
     2281 gaattagtag atgcgagaaa aaagcctata actcctacaa tggatattta cctcgatgaa
     2341 gaacatcgta tttcaagaga aaaagcctta acggttgcaa aagagataat acatactaag
     2401 gttattgatg tagtcgaaaa aacagatatt gattatagtg gaatcataaa attgaccttt
     2461 tctgagaata aattaaatga cagaggttgc attttaacag atatttttga aattttaaag
     2521 ggctctaaaa agaaatatga taccaccctt gatgaaaacg aaaagagtat agttttaaaa
     2581 atgaatgagg aattagatgc acaaacctta ttacttttga aaaataagct cctcaatacc
     2641 aaaataaaag gtgtacccga tattgaaaga gttacaatag ttaaacagaa tgaggaatgg
     2701 gtaatacaaa ctgctggatc taatttatct aaaataattt atgtagatgg aatagatctt
     2761 tcacgagtta caacaaataa tgtttatgaa atatggcaaa ctttaggaat agaagcagcg
     2821 cgaacagctc tagtacgaga agttactaat actctggaag aacaaggtct tgaagtagat
     2881 atccggcata ttatgttggt agctgattta atgacgtcaa aaggctacct tcagcaaatc
     2941 ggaaggcatg ggatcgccgg aactaaaacg tctgtattgg ctcgtgctgc ttttgaaatt
     3001 actgttccaa ctattgcaaa agcctctatg gaagggcaaa cggaagcatt aaaaggagtt
     3061 accgaaaatg taattgtggg agcaactgta cctgttggaa caggaatggt tgacttgtat
     3121 atgaaagtga atgaacaaaa tgaaaaatct ggagaaaata attaaagacg caaaatcgtc
     3181 taacaagttt aaggaaggat taaaagaagt ccgatcgaat attaaaggat caaaattaat
     3241 tatagtttct aactctctcc tcaattctga taaacaattt atcgaaaaga gctgcaaaga
     3301 attgaaagtt cctctttatt attatggtga caattcgata aaattagggc gcctttttaa
     3361 taagccttat agaatttctt cagtagcact caaatcagta gaagacgacg atgtaactgc
     3421 attgttggca acacagtaat ttttttattt ttaaattttt gaccactaga agatagaaca
     3481 tcataatatt ttaataattt gatttctaat ttggaatatg gactatcaat ataatacaga
     3541 cgaagaaaat tatctttcaa caaagaaatt gtttgtaatt ttaggcttat ctgtctttgg
     3601 agtttttatt gttttagctt tgtttgtatt tccaatcaat aatttaataa gagaaacagt
     3661 ctctgaacaa gcaattataa tctctaaaag cgagaatata tgtgtagttg aaacattgga
     3721 tcatccaaga caaatcgata attgtgttta tgcagtagga gatttagtat acgttacctt
     3781 tgacgcagga actcagagaa tcaaagaaca caaactttta catgaataaa atttatttac
     3841 cttgatctca gttaatcata atctcttgtc tctaaaatat tttctccagg acttcctagg
     3901 atcttcatta aaatttaaat tatacttgat tacacagcat gttattattt gatgtataac
     3961 aattcaaaat tggatttaga ggtattgtgg tataatgact gaaaaaataa aattaacatc
     4021 tgatgaactt aggcttatct ctttatttca gagcattact agtgctaccg caagagattg
     4081 tatagtagat gaaaaaatgg atagagtgat atttgttgta aataagggtc aaatgggctt
     4141 agctattggt aaaggaggaa ccacaataaa acaattacaa aatgttattc cacgaagaat
     4201 agaattagtt gaatattctg ataatgaaga tgaattttta aaaaatattc tcaattccaa
     4261 tatgattaat gaaataaaga ttaataaaag gatagatggt acaaaacagg caattgttgt
     4321 tgttgattca aaaaaaaagg gtgttgtagt tggaaaggaa ggtagaaatg ctgagaaagc
     4381 taggttactt gcaaagcgtt atttccacat atcaaatgtt ttaatattga gtcctgaaca
     4441 aacaactcat gataataatg gtgtgaatgt gtaaatggct aaatctcctt tgggtttgtt
     4501 cgctggacgt gtacttcaat caaaaagaaa acgttctaga tgggcaaaca aatattataa
     4561 aagacgagta ttgttacttg acaagaaaac taatcctatg gagggagctc ctcaggctag
     4621 aggaattgtt ttagaaaaag tcggtataga atcgaaacag cctaattctg ctgtaagaaa
     4681 atgtgtcaga atccaattaa taaaaaatgg aaaatcaata actgcatttc ttcctagaga
     4741 tggggctttg aattttgtgg atgaacatga tgaggtcata gtagagggta ttggaggttc
     4801 aatgggtggt gcaatgggtg atatacccgg agtacgatgg caggtattta aagtaaatgg
     4861 tgtatcatta aaggaattag tttacgggag aaaggaaaaa cctaggcgat aagtatgagc
     4921 ggcaaagaaa atattaaaat attgttgttt aacaaatggg atacagctaa tattgaaatt
     4981 aatgatcctg gtttaacacg ggttatttct ctaaatccat caatggtaat tcctgtatct
     5041 tttggcagac atgaacacca aagactcaaa aaagctgagg taaaccttat tgaaagatta
     5101 tcaaataaat taatgcgttt cggaaaaaaa tatgcaaaga atactggtcg aatgggtgga
     5161 aaaaaagcaa aatcactaaa tgttgtcaaa accgcttttg aaataattca tctagaaacc
     5221 ggaaaaaatc cagtagaatt attagtaaga gctatagaga actcttctcc aaatgaggat
     5281 actacgagaa ttgtatatgg tggtgttgtc tatcatgttt cagtagatgt ttctcctctc
     5341 cgacgactcg atttagcact tcgattcata gctgaaggtg taagagaatc tacctattct
     5401 gctccacaaa tgatcgaaga agcgttagcg aaagaaattc ttcttgcttc aaacaatgat
     5461 atgtcaagtc atgctataaa gaagaaaaat gaacaggaac gtattgcaat ggcttcaaga
     5521 taaacatttt ttaatttttc tatttttaag cttaattatt cttattacaa ttttatcaaa
     5581 acatgcaagt taataaataa tggcaattat tactccagtc attataaatg agtagcaaag
     5641 acgattttgt tttttctaaa ggtgctttaa tgagtacaaa agctggtaag gagatactta
     5701 aacaggccat cttaagagag aggggctata aacaatataa taaatacaaa cttaagtatg
     5761 aaactgaatt tgagggtttc accaagcgct ttcttctttc gttacataga cgcataattt
     5821 ctgacacatc tccaaattca acagtagctc aatttgcaga agaagttggt tcccacgata
     5881 tggaacttga ctcctcaaaa gttgaagata ttaaaactcg tctttctaga ccggaaattc
     5941 ttgcagatag agtccaaaga atattggact ctaattttgt taaaatgact tttcctgtgt
     6001 ttaatgcttt atttgatgga tctgtgtctt atcttaaaga agatatttcc aatgaactac
     6061 gaacttctat aatagatggt catatcatag ctatagactt gagtgaacct atggatagaa
     6121 ttatggataa agatgaagat ctcgaatatt tagatgacta tcgattaatg aatccatata
     6181 ttttggatat agcaagggaa aaaatttctc aaggaggtga ttctgtttta aaggcttttg
     6241 aagatggatt taaggacgct cgaataggtc aattaattga ccataaacta aagatgaaac
     6301 ctgaatcgat tactgatgaa cttatgatcg gttgttataa aaaataccgc tccatcatgg
     6361 gtactgctgg cagaaatatg gccttaaatc agacaccatt aagtgaaatt tatcatttag
     6421 gaatgtcgaa agctagtgaa tcagtaggct gtgggaatga gatgcaagat gcaatcaatg
     6481 aaggctcaat caagatacct tcttggcctt tgtactattc acttattgct aatgatgtta
     6541 ggaaagggtt tgaacttaca ctcaaaaaaa gcgagatata tttggatgaa gcaaagcttg
     6601 cactggaatt tcttcctgaa gaatttgaac ttaaaccatt tctaatattt ttatttctaa
     6661 cagtaagcca ttataatcag tactggtatc atgacatcac taaaagggat ctctttaaat
     6721 attttgaaag aactattcat cccctaacaa gaaaattcat ttagtttata ttttgatata
     6781 tatattatgt attgggcaga aacataccga cctaaaaatt ttgaacatat tattggtaat
     6841 gaagaaatta ggcttaatat actaaaatgg ttagtaaaat ggttacctgg aaccaaacct
     6901 ttacttttaa taggaccgcc aggagttggg aaaacaacaa ttgttaaaat attatcttca
     6961 cttttgaact atgatctaat agaattaaat gccagtgata ctagaagtgg gacacaatta
     7021 gaaaaattaa taactccact ttataataac acgagtattt ttggaaaaaa aatacttatg
     7081 ttttttgatg aagtagatgg tatatatgga agagaagata gcggcggact cgaaattctt
     7141 atcaatattg taaaagcagc aaactttcca ataatatttg tggctaattc taataatatt
     7201 aaattaaaga atttatctaa actatgcaaa atatttaaat ttcaacatat accagcaaac
     7261 ctcttgatga tgtttttaaa ttatgtatta tttaaacaag gttattctct cactactgag
     7321 gataaaaaat ggatagttga aaaaagtaat ggtgatgtaa gaactatgct taatctggtt
     7381 cagtctaagt gcactggcta cgacaagttt gtgcatgaaa catttaaagt agatatttct
     7441 gaaggtctaa atgaattttt ccatagtaaa cagaagggtg atgctaaaaa aatcttgtta
     7501 actttagatg gcaaataccc tgatcctcga tatggtttat cttctgaaga acggagaaaa
     7561 gatattgtca attcgttatt tacaagcttt gtatcgtcta acctagatat tgatgattta
     7621 tctctactct tagatagact ttctcaaatt gatttacatg tcgcacggat aaacgaaaat
     7681 agaaactgga atttattaag atatatgaat aatgtaataa tccaactctt gtatgaaaat
     7741 atacaaaata aaatgatatc ttataatcaa tatggaatta gttggcctgt aatgggtcaa
     7801 gtattttcca gaggtgtacc gttacgtagt ttgatttcta agttatcatt gtcgtttcac
     7861 gtttctacta gtacttttgg aatgatattt tttccctata tactccaaat tattcatgag
     7921 tctaaaatag atatccatga cttggtttcg aatctcatgt tagatgaaaa atttgtagaa
     7981 attattcaaa aagaaatttt gaggtctaaa agatatgagc gaacctgatt ggataaaatt
     8041 attggtaaaa tttaaaggaa ggtgtcaaag ctgtgaaaac tcaatttcgc ctggcgaata
     8101 tgctttatgg tcaaaatcat caaaacaaat caagcatttg gattgtaaga tcaatgacaa
     8161 caagaagacc aagaaggaaa aagtggagaa atattcagaa ttacaattac agtgtttttt
     8221 gtgcaaaact gttgaaaata tacctatttc tttactaaag gaatatttat atcatgaaac
     8281 tcaagaaaat tctatattct tgtgtagatc ttgtttggaa aatcatagct cttatttaat
     8341 atatcatgag ataatgtatg aaaaagtaaa aaagatagta aaattaaaat tttgataaat
     8401 gcgagatcaa tacaatgtca agtgaatatg aacgcactat tgcaaaattg ctatttgaaa
     8461 aaaagcaaaa attggatctt ttattagaaa atccacagga aaataaatat caaatacaat
     8521 tattgtctac tgaaattgag acattacaac caatattcga aaactataat cttggtatga
     8581 atgtttttag aagagctcaa ggtggacgct ctcacttacg tgagtaagtg ttattcttca
     8641 agataagact agtacttaaa taaattagaa tttgattctt catccactaa agggtttttt
     8701 atcagtattt tttgttatac atatatacta gataatcggg ataaatgctg aagccatgca
     8761 cgaagataag ttaaagaaac tatatgaatt gaaaaattta gctgaatctg ggggtggtaa
     8821 agaaagaatt gatgctcaac attctaaagg aaagcttacg gcgagagaaa gaatcgatct
     8881 tcttcttgat gaagggagtt ttatcgaatt ggacaaatat gtgactcata gaagtgatga
     8941 tcctgaaact caaaaattct atggagacgg agtaataact ggatttggtt caattaaagg
     9001 tagaaaaatc tttgtatatg catatgattt tacggttttg ggtggttcat tgggagaaat
     9061 ggcatctaaa aaaattgtaa aaatcatgga tagagcaata aaagtaggtg cacctcttat
     9121 aggaattatt gattcaggtg gagctagaat tcaggaagga gtaatgagtt tagatggcta
     9181 cggtgatata ttttacagga attgcaccgc ttctggaata attccgcaga taactgctag
     9241 cattggtcct tgtgctggtg gtgctgtata ttctccagca atgacggatt ttgttataat
     9301 ggtagaaaat ctaggtcaga tgtttgtgac tggaccagaa gtggttaagg aagttttaag
     9361 tgaagatgtc tcatttgaag aactcggagg aacttctaca cacgcaacaa aatctggggt
     9421 agctcatatg gttgcagcta atgagtatga atgtatggag aaaattaaaa aattaatatc
     9481 gtttttacct caaaacaatt tacaagaacc accttcctta gagacaaatg atgatccaaa
     9541 tagactggat ccatcattaa ttgatatgct acctgaaaat ccgtatcagc catatgatat
     9601 taaatcaatc ataacaagta taatcgatga taaagaattt tttgaaattc acgaaacttt
     9661 tgcaagcaat attgttgtag gcttggcaag aatgggtggt aaaaccgttg gtattgtatc
     9721 caaccagcct atgtatttag caggtgtttt agatattgat tcctctaata aagcagcaag
     9781 attcattaga ttttgcgatt cttttaatat tcctattatt acattagttg atacacctgg
     9841 ttatttacct ggaactgatc aagagcaaaa tggtattatt agacatggta gtaagttatt
     9901 gtatgcttat tgtgaggctt cagtacctaa aattacatgt atcattggaa aggcgtatgg
     9961 tggagcatat attgccatgg ggagtaagaa cctaaaaaca gatattaatt ttgcatggcc
    10021 ctctgctgaa attgctgttt taggtccaga agcagcagtt actataattc atagaagaga
    10081 acttaagagt tctccagatg caaaaacaat taaaaagaaa ttagcagaag aatttaggaa
    10141 aaaatttgct aatccatata ttgcagcaga gaaaggaatc attgatcagg tcattgatcc
    10201 aatggaaact agaactatga taataggtgc ccttgactca ttatccggaa aaagagagat
    10261 aggtccttgg aaaaaacatg ggaacataaa tttataggtg aacttgagaa tgccaaagat
    10321 ttctagcatc ctgatagcaa atcgtggaga gattgccctc cgtgttataa gagcatgcaa
    10381 agagttggga ataaaatcaa tttcagttta ttcagatgac gataatagat caatccatgt
    10441 taaacgagct gatgaagcat atagacttgg accttcccaa gcttcggaaa gttaccttcg
    10501 tatagacaaa atacttgaaa ttgctaaatc ttctggggcc gatgctatac acccaggata
    10561 tggatttcta tccgagaacg aagttttttc cgatgaatgt aagaaaaatg gtattatttt
    10621 tataggacca acagcaagag cgatggagtt agcaggtgac aaaatgaaat gtaaagaaat
    10681 catgaaaaaa gcggaagttc ctattgcacc aggaagtgat ggagtagttg atgatataga
    10741 attggctgtg gaaatagcta aggaagcacg ttatccggta atgctaaaat cagcttttgg
    10801 aggaggagga cggggaatta gactcgtaaa taatgaaaaa gaacttaggc aagaatttga
    10861 actagcgtcg aatgaatcac aagcagcctt tggtaaatct gcactattta tagaaaaata
    10921 cctaacaaac attagacaca ttgaatttca attgataaga gattctagtg gaaatggtag
    10981 acatcttttc gaaagagaat gttctattca aagaagacac caaaaactta ttgaaatgtc
    11041 tccttcccca gttgtaaata aggagaggag agagaaagta ggcgaaattg ctgttcgtgc
    11101 atctactgca cttgactatt taaatgctgg tacagccgaa tttttggctg atgcggaagg
    11161 aaatttttat tttattgaaa ttaatgctag attgcaagta gaacatccaa tcactgaaat
    11221 tgttaccggc aaggatttgg ttaagttaca aattcatgtc gctcaaggtg aagaactacc
    11281 atttaaacaa gaggatcttg aattaaaagg ttctgcaata gaatgtcgta tcaattcaga
    11341 agaccctttt tatgatttcg ctccttctgt aggatatgtt ccaaattgta atattccggc
    11401 tggccctggc gtgagagttg atacttatct ctaccctggt tgtaatgttt ctgggtatta
    11461 tgattcatta attgctaaat taattactta tggtggacac tttaatgaag cacgcatacg
    11521 aatgaagaat gctttatctg aatttaccat tgaaggaata aatactacga taccaattca
    11581 caaaacaatt atagaagaat caaattttat taatggcaat atttctactg attatatcga
    11641 aaaatataat ataatcgaaa agatgaaaca taaaagaact caggaataca aagaaaaagc
    11701 atatgcagcc atttctggaa ttctattaca gtctgaattt gtaaaaaaat cttatttagc
    11761 tcacagaata gatagaggtc aaaagtcaat ggatgcagga ggattttttt aatggaattt
    11821 caaatagaag gtaatgctaa taaaattaat gctgagttag tttacaataa aggatcgggt
    11881 gatcttttat ttgttataga tgaccagcaa tataatctaa aacttttaaa atctacagcc
    11941 aatgaatttg aatttatttt agacaataca tatcattatg ttaaaatact tccttctacg
    12001 gaaacaaaat ataaaattta tatcgatgga atagaagttg aaattaagaa acattcaaaa
    12061 ataacagaaa taatagaaaa aacattaaag gctaaaggta ctgcttatca agctaatagc
    12121 gtagctagcc aaataccagg tagagtggtt aaggtctctg tttcaaatgg tgataaagta
    12181 aaggaagggg aacccattat ggtccttgaa tctatgaaaa tgcaaattgc tataaaatct
    12241 cataaagagg ggattataaa ggaaattaaa gtaaaagaag gatcaacggt ctcacgaaat
    12301 gaaattgttg caataataga gtaggcatta tatatttcaa aatatttatt atacataaaa
    12361 ttatctttac aaggtcaaaa gaccttggtt aataagttgg tgatctgaag tccaacttat
    12421 attatcttat tttctattgc gcttatataa aaaaggattt tcaatgcttt gtatattttt
    12481 aagtgattta gatctttcta attccattat gttcctcttt catgatctat tgtagtcact
    12541 taagaatgat gcagtatgct tcaagtcttc tttgaaaaat tgaataaaag gatcattaac
    12601 agatagttga attaaattaa tatgagttaa tcatttagtc aattttaaat taaggcattt
    12661 catcattata accttaaaat tttactgttg tgaaaaaagg aagtacaatt tgttatcatt
    12721 taaatcgctt aatttatttt tcatttagta ttaactaagt caaagataga gacatatcct
    12781 aggttttaaa aaattcttcc cagttgacac tttcaaacct tgataaatgt ataatcgcgt
    12841 ctagtattaa tctctatatt gcgtaggaga aaattttcta agctctttga ttctacgact
    12901 caaacttcta atagttttat ctatttcttt cttggtattt tgtattccta aagtaaacct
    12961 aattgaacct gaaatctcct catgattcaa accaatagct ttcaaaacat gagactcttt
    13021 ttgcaggttg gcagagcaag cagagcctgt agatgccaat attccatctt catctaattt
    13081 aattagtaaa tcttcaccat ttatccctaa aaaagtaaaa ttgacattat tgggtaatct
    13141 actttcaagg gagccattta acatactacc agagatttcc tcatttattc tattaataaa
    13201 gtaatctctt aaataatata agtagtgaaa attttctttt agattcagca tgcttatttc
    13261 tgatgctttt ccaaaaccta caattccagg aacattttcg gttcctgatc taaaggttaa
    13321 ctcttgtccg cctccttgaa ttaaaggttc aaaatttacc ttttcacgca cgtataaggc
    13381 acctactcct tttggcccat taattttatg cgaagacaag gataatgaat caagatccaa
    13441 ttctttaaca tttaccggta tttttcctac cgcttgaacg gcatcagaat gaaatattat
    13501 ccttttgtca aagttatgaa caatttctga gaattctttg attggttgaa taacaccgat
    13561 ttcattatta gctagcatta tactgactaa cgctgttttt tccgtaagat g
//
LOCUS       DATAFW010000030        11092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2828204, whole genome shotgun sequence.
ACCESSION   DATAFW010000030 DATAFW010000000
VERSION     DATAFW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2828204"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1451
                     /gene="hemA"
                     /locus_tag="VHJ57_00630"
     CDS             156..1451
                     /gene="hemA"
                     /locus_tag="VHJ57_00630"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX2487071.1"
                     /translation="MTLVNKSFELLNFRITYKEAPIHMLEKFSFKDVNSAHETFLEHL
                     QMKECLILQTCNRVEIFAVIEEELNLQKIISYWADHSKLSTEEFSNIIEIVKGEEVIR
                     HLLKLVAGLDSLVIGEDQILGQVKRSYEFSRSNNYLGSFLSIIFEKSLRVGSKVRSLT
                     GINKGRVSVGSVAVSLAEESIGRLNDKKILLIGSGEGASLIAKALKQRNVSFMNTSRT
                     YERAKSFAESLSGIPIMFEDALISLHSMDIIFVSTTAPYYLLTHDRIKKTMDTKNTSL
                     LIFDLSNPRTVEDSISQIDNVFLFNLDQIGGIVQRNLLWRKEEIRSADKIIEEEMKNM
                     DLLLKRRKADPVVVSVFRNVDKIRKRELNKALSILGPKIGPQEGKIIEQFSHALLEGI
                     MSTPMNNLRRQLQNDKDDEQELMRLAAKLFNYENEANLQ"
     gene            1426..2448
                     /gene="hemB"
                     /locus_tag="VHJ57_00635"
     CDS             1426..2448
                     /gene="hemB"
                     /locus_tag="VHJ57_00635"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX2487072.1"
                     /translation="MKMKQTSNNSLANGPYFPNVRLRRLRKSKALRDLLQETRISSDD
                     LIFPLFVEEGLKNPKNVASMPDIQRIPPSKIVDVVSEILDLGIKGVIVFGIPSKKDNT
                     AQAAYNEKGVVQKAIKTIKKNFGDRIVVISDVCMCQYTTSGHCGIIRENVVDNDSTIE
                     MLSKIAESHAKCGADIVAPSAMMDGQVFAIRKQLNDAGLADTSIMAYSAKMASPLYTP
                     FRDLADSTPKFGDRKTYQMPGTNSFESLREIELDIKEGADMIMIKPALPYLDLIYKTR
                     ELFNLPLCAYSVSGEYALIKAASEKGWINETDVMIEFLSSIKRAGSDLIITYFAKKMA
                     KVLLEK"
     gene            2482..3762
                     /locus_tag="VHJ57_00640"
     CDS             2482..3762
                     /locus_tag="VHJ57_00640"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX2487073.1"
                     /translation="MINDRSEKLFQRAIKVIPGGVNSPVRFFRPYPFFVSSGLGSKIV
                     TADHQTYLDYCMGYGSLIFGHAYLPILESVKTQLDNGTLFCIPSEKEIYLAELLTKII
                     PCAERVRLVNTGAEATMTAIRLSRAFTRRKKILKFKGGYHGAYDYVLVKAGSGAATIP
                     SSDGSLQEISEETLLVEYNDFGDLERIIKKESENISCLIMEPVLANYGLLLPEKNYLT
                     EIRKITKKYGILLIFDEVITGFRLAVGGCSEFFGITPDIATFSKSFGNGFPIAAICGK
                     KEIMDQLSPSGNVYQASTYAGNPISVTAAITTVESLLKNKNKIYPTIARLCDTLGMGI
                     KDGVKEFAEHYTINSIGSLFQIFFTADAVSTPLDILKCDTKMFENLFWDLLKKNIFIP
                     PSMYETCFISYSHSDEDIEKTIECYSDSLNKYTK"
     gene            3764..4717
                     /gene="hemC"
                     /locus_tag="VHJ57_00645"
     CDS             3764..4717
                     /gene="hemC"
                     /locus_tag="VHJ57_00645"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX2487074.1"
                     /translation="MTRKLRIGTRGSKLALAQTQIVIKALSHQNETIEYEITPIKTRG
                     DLDNSKPLFYLDKKGIFEKEINESVVKKDVDFAIHSVKDVPATIHEDLVIASIPQRES
                     VIDVLISKSRKRLDQLPIGSIIGTSSLRRAIQIISFRNDFKVIPLRGNIDTRIEKALK
                     GEYDAIILAEAGLKRLGKEQYISERFDPKKFLPAAGQGALALVCRKTDRKLVKFLHKI
                     EHVPTRISINAERSFISKIEGGCRFPIAAYTNLSPKNHKINFYAKIFSSDGLEHISIK
                     KIGDYDQPEIVGNMVASYLIANGALRLAEGWDKSLNEWNKK"
     gene            4698..5459
                     /gene="cobA"
                     /locus_tag="VHJ57_00650"
     CDS             4698..5459
                     /gene="cobA"
                     /locus_tag="VHJ57_00650"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX2487075.1"
                     /translation="MNGIKNKKYGKVYICGAGPGDPSLITVKCWDLIKNSDIILYDRL
                     VSSGILKLIPEDTPKVYVGRSSGDPTINQFKTNQIMLQYALTGKQVLRLKGGDPFIFG
                     RGGEEAEFLNENAIDFEIIPGISSAIGAAIYSGIPLTHRKFSSSVAIVTGHEDPTKEE
                     KSVRWDKIATAVDTIVILMGVEKLNSILKKLKKHGLPDETKIAVIEKGTLKEQKIIIG
                     NFNNIKKKMKNASIQPPAVIIIGEVVSLSERLRWI"
     gene            5450..6274
                     /locus_tag="VHJ57_00655"
     CDS             5450..6274
                     /locus_tag="VHJ57_00655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX2487076.1"
                     /translation="MDLIYYGSIDHNFKIIAITKSEGSISKKFIDKIEKNSSRLIVVK
                     TSNIVRSDSKVFIQIFRLIANWSSGYIIFLSSNAVDVFFEFAETLNIISDIVYDLNSK
                     FNVVAIGPSTRTSLKKHRILVNSIPHDHSSLGIIDFFSQLKNSKYESKIIIPRSKKAD
                     KFLKEKLVKLGFVVNEFYIYNVTPAKVDPVWIEFIDLLKKEKIDSLIFTSPSNVRYFI
                     NIIKKHSSDLLPLVCKIKVIVSIGPLTSKELTKYNIPFSESTNHSLEGVYENLYMK"
     gene            6363..7772
                     /gene="sufB"
                     /locus_tag="VHJ57_00660"
     CDS             6363..7772
                     /gene="sufB"
                     /locus_tag="VHJ57_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX2487077.1"
                     /translation="MKYMTTEELNMDYSKYDFKDSTDLYVYTSKKGLSRGTIEEISRI
                     KNEPQWMLDFRLRSYDIFMNKPMPVWGGDLSKINFQNIFYYTKASEKQSKSWDDVPDS
                     VRKTFDKLGIPEAEKKFLAGVGAQYESETVYHNLREDLSKQGVIFSDTDTAVKEHPEI
                     LKKYFGKVIPPEDNKFAALNSAVWSGGSFIYVPPNVKIDLPLQAYFRINAENIGQFER
                     TLIIADEGSEVHYIEGCTAPVYTTESLHSAVVELIAKKDAKIRYTTIQNWSKDVYNLV
                     TKRAYAYENARVEWIDGNIGSKLTMKYPGVYMLGRNAHAEVISIAFAGSDQHQDAGAK
                     AVHLAPDTTSRITSKSVSKQSGRTTYRGLLHVAKGATGVKSNVRCDALLLDETSRTDT
                     YPYVEVNEDDATISHEASVGKIGEDQIFYLMSRGFSESDALSLIVGGFIEPFTKELPM
                     EYAVELNRLVKLEMEDSVG"
     gene            7839..9245
                     /locus_tag="VHJ57_00665"
     CDS             7839..9245
                     /locus_tag="VHJ57_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HEX2487078.1"
                     /translation="MLEKNFDNFIDNFREPPWMKQSRYEAFKSYTSLPLESSALYSKY
                     SNANKLIAEKVFFNDTNLQGESSNDLDIFSDRMDEFNKTTSVLCINSKIHKSFVTDEL
                     EKKGIVILNTSQALRDYSDIIQKYLNKNKLNYNEDKFLALSSAAFHDGLFILIPKNIV
                     LEDPIRIVNYLSEDGLSSISRNIVVCEEFSKATLVQELYSQRSKHPQYQQTHFELLES
                     HILPNAQCEIVTLQSMDYDAVSFSNRKAYVERDGKMSWSLALFGSQLSRYKVDSIMQG
                     QGALAEDVEIIFGVGNQSFDITSNLIHVAPNTRGKVLVKSVMKDTSQSLFKGMIKIHT
                     NAKATESYLAGHAILLDKGAKSDAIPGLEIESNEVKATHSASVAQIDENQIFYLMSRG
                     LSEELAKREIVGGFLEPISRKMGPTIRAWINYLIENKWLGKPLKLKIDEAMEQILEIE
                     KSRYRETEDIFEKHYKYR"
     gene            9279..9593
                     /locus_tag="VHJ57_00670"
     CDS             9279..9593
                     /locus_tag="VHJ57_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX2487079.1"
                     /translation="MSDWVRVCDLDSLKPGELMDFDIDDKKILLVNIKNTIYATDRIC
                     THAYADLSMGFLNEEEKSVTCPLHMSAFNLNDGRPLNLPAEEPLTVYKTKIDNNSVFI
                     LV"
     gene            9844..10722
                     /locus_tag="VHJ57_00675"
     CDS             9844..10722
                     /locus_tag="VHJ57_00675"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX2487080.1"
                     /translation="MKIGIIGLGLMGSNLCLRLIRKGFQVNVHNRNVSKSLAIEKKGA
                     KVFNTPKEVADYSDFIITCVTNFEAVNKIYFYKNGVSETKNKNVIVSDFSTLSPKDSV
                     YCYQKFKRKNITMMSIPVMGGPNAALTGTLIPIVAGEEISFKKIKYVLQELGDPIFYI
                     GNKAGSASAIKLALNLNIGIIAMALSEGLLLAERYGIDPNLYLKILNCTNFKTGLSEN
                     KGPKMIKGDYSPSFYLKNMKKDLALIMEAAKEQDLSLPITGLVFQFYNYASKSIFADL
                     DYSVIYKFVRNLQSLE"
BASE COUNT         4060 a         1562 c         1949 g         3521 t
ORIGIN      
        1 gcgagaccca aaattaaaac cgttatagaa cgtaaaagat ttctttattc aataataaaa
       61 gataaaaata tccaaaattt aattaatgag aacaaactag atgaagcgaa ggttgccgct
      121 attgagcgct taaatcattg gagtgatttg aaaaaatgac cttagttaat aaatcttttg
      181 aattactgaa ttttaggata acttataaag aagctcctat acatatgctt gaaaaatttt
      241 cctttaaaga tgttaatagt gctcatgaaa cttttcttga acatttgcaa atgaaagaat
      301 gtttaatttt acaaacttgt aatcgagtag aaatttttgc tgtgatagag gaagaactaa
      361 atttacagaa aattattagt tattgggcag atcattcaaa attatcaaca gaagaatttt
      421 caaacataat agagattgtg aaaggtgaag aggtaattcg acatctacta aaactagttg
      481 caggtttaga ttctttggta attggtgaag atcaaattct aggccaagta aaaaggtcat
      541 atgaattttc gcggtctaat aattaccttg gatcttttct ttcgatcatt tttgaaaaat
      601 cattacgggt aggaagtaaa gtgcggtcac tgacaggtat caataaagga cgggtatcgg
      661 tgggatctgt agctgtcagt ctagcagaag aatcaattgg aagacttaat gataagaaaa
      721 tactgcttat aggctcagga gaaggtgcaa gtctcatagc caaagcacta aaacagagaa
      781 atgtatcttt tatgaatact agtagaactt acgagagagc taaatcattt gcagaatccc
      841 tttcgggtat acccataatg tttgaagatg ctttgatttc tttacattct atggatatta
      901 tattcgtaag tactacagct ccatattatt tacttacaca tgatagaata aaaaaaacaa
      961 tggacacaaa aaatacatca ttattaattt ttgatttatc taaccctaga acagtagaag
     1021 acagtatttc tcaaatagat aatgtctttc tatttaatct agatcaaatc ggaggtattg
     1081 ttcaaagaaa tcttctatgg agaaaagaag aaattcggtc tgcagataag attattgaag
     1141 aagaaatgaa aaatatggat ttgttattaa aacgaagaaa agcagatcca gttgttgttt
     1201 ccgtttttcg gaatgtagat aaaattagga aaagagaact caataaagcc ctttctatcc
     1261 ttggaccaaa aatagggcct caagaaggaa agatcattga gcaattttct catgcacttt
     1321 tagaaggaat aatgtctact cctatgaata atcttcgtcg gcagctccaa aatgataaag
     1381 atgatgagca ggaactaatg cgtttagctg ctaaactgtt taattatgaa aatgaagcaa
     1441 acctccaata attctctagc taatggacca tattttccga atgttagact aaggcgtctt
     1501 agaaaaagta aggctcttcg tgacctttta caagaaacgc gtatatcatc agatgattta
     1561 atttttccat tatttgttga agaaggatta aaaaatccaa aaaatgtagc ttccatgccc
     1621 gatatacaaa gaattccccc ttcaaaaatt gtggatgtag tttcggaaat tttagatcta
     1681 ggaattaaag gagtaatagt ttttggaatt ccctctaaaa aagataatac tgcacaagca
     1741 gcatataatg aaaaaggagt agttcaaaaa gcaattaaaa ctataaagaa aaattttgga
     1801 gatagaattg tagtaatctc agatgtatgc atgtgtcagt atacaacttc aggtcattgt
     1861 gggataatta gagaaaatgt ggtagataat gattccacta tagaaatgct ctctaaaatt
     1921 gctgagagtc atgccaaatg tggtgcagat attgttgctc catccgctat gatggatgga
     1981 caagtttttg caattagaaa acagctaaat gatgctggtt tagccgatac atccattatg
     2041 gcctattcag caaaaatggc ttccccatta tataccccct tccgcgacct tgcggattct
     2101 actcctaaat ttggcgatag aaaaacatat caaatgccag gtactaattc ctttgaatca
     2161 ttacgagaaa ttgaattaga tatcaaagaa ggggctgata tgattatgat aaaacctgct
     2221 cttccctatt tggatttaat ttataaaaca agggagcttt tcaatcttcc actatgtgct
     2281 tacagtgttt caggtgaata tgctttaata aaagcggcct ctgaaaaagg atggataaat
     2341 gaaacagatg tcatgataga atttctttcc tcaataaaaa gagccggatc tgatcttata
     2401 ataacttact ttgccaaaaa aatggcaaag gtcttattag aaaaataaga ttcttataaa
     2461 tagattataa taattacata cttgataaac gatcgctctg aaaaattatt tcagcgagca
     2521 attaaggtga ttcctggagg agtgaacagt cctgttaggt tctttaggcc ttacccattt
     2581 ttcgtctctt cgggtttagg aagtaaaata gtgactgctg atcaccaaac ctatctagat
     2641 tattgtatgg gatatggatc cttaatcttt ggacatgcgt atttacccat tttagaatct
     2701 gtaaaaactc aactcgataa cgggactttg ttttgtatcc cgtctgaaaa agaaatttat
     2761 ctcgctgaac ttctgacaaa aataatacct tgtgcagaaa gggtaagatt agttaatact
     2821 ggtgctgaag caacaatgac cgctatacga ttaagtcgag cctttaccag acggaagaaa
     2881 atacttaagt ttaaaggagg ataccatgga gcatatgatt atgtcttagt aaaagctgga
     2941 tcgggagcag caacaattcc atcatctgac ggctcactgc aagaaatttc agaagaaact
     3001 ctgctagtgg aatataatga ttttggagat ttagaaagaa taattaaaaa agaatcggaa
     3061 aatattagct gtttaattat ggaaccagtc ttggctaatt atggtctttt acttccggaa
     3121 aaaaattacc taacagaaat tagaaaaatt acaaaaaaat atggtatctt gctaattttt
     3181 gatgaagtca taactggatt taggttagct gtaggaggtt gcagcgaatt ttttggcata
     3241 acacctgata ttgctacctt ctctaaatct tttggaaatg gatttcctat agctgctata
     3301 tgtgggaaga aagaaataat ggatcaacta tctccttctg gtaatgttta tcaagcaagc
     3361 acatatgcag gaaatcctat ttctgtaaca gcagcaataa caaccgtaga gagccttttg
     3421 aagaataaaa ataaaattta tcctactata gcgaggctat gcgatacgct aggtatggga
     3481 attaaagacg gtgtaaagga atttgctgaa cattatacta taaattctat tggatctcta
     3541 ttccaaatat tttttaccgc agacgcagta tctactcctc ttgacatttt aaaatgtgat
     3601 accaagatgt tcgaaaatct attctgggat ttactaaaaa aaaatatttt tattcctcct
     3661 tctatgtatg agacatgttt tatttcttat tctcattctg atgaagatat agaaaaaacc
     3721 atagaatgtt attctgattc tttaaataaa tatactaaat gaaatgaccc gaaaattacg
     3781 tataggtact agaggaagta aattagcctt agcacagaca caaattgtaa ttaaagctct
     3841 tagtcaccaa aatgaaacta tcgagtatga aattactcca atcaagacca gaggagattt
     3901 agacaactcg aaaccattat tttatctaga taagaagggt atctttgaaa aagaaataaa
     3961 tgaatcagta gttaagaagg atgttgattt tgctattcat agtgttaaag atgttccagc
     4021 tacgattcat gaggacttag tcatagcgtc tattcctcaa agagaatcgg taattgatgt
     4081 tctcataagt aaaagtagaa aaagattaga tcaacttccc atcggttcga tcataggaac
     4141 aagtagctta cgaagagcaa tacaaattat ttcgtttaga aatgatttca aggtaatacc
     4201 attaagaggt aatatcgata ctagaattga aaaagcctta aaaggagaat atgatgcaat
     4261 tattctagct gaagcaggat tgaaaagact gggaaaagaa caatatatca gtgaaagatt
     4321 cgatcctaaa aaatttttac cagctgccgg acaaggggct ctagccttag tatgtaggaa
     4381 aacagatcga aaactagtca aattccttca caaaattgag catgttccaa ccagaatttc
     4441 gattaatgct gaaagatctt ttatatcgaa aattgaaggt ggctgtagat ttccaattgc
     4501 agcatacact aatttatctc ccaaaaatca taaaattaat ttttatgcta aaatattctc
     4561 ttctgatggt ttggaacata tttctataaa aaaaattggt gattatgatc aaccggaaat
     4621 tgtcggtaat atggttgcta gttatctaat agcgaatgga gccttaaggt tagcggaagg
     4681 ttgggataaa tctctcaatg aatggaataa aaaataaaaa atatggtaaa gtatacatat
     4741 gtggagcagg accaggcgac ccatcactaa ttacggtaaa atgctgggat ttaataaaaa
     4801 attctgatat aattctatat gaccgcttag ttagtagtgg cattcttaaa ttaattcctg
     4861 aggatacacc aaaagtatat gtaggacgtt cgtctggtga tcctaccatt aatcaattca
     4921 aaactaatca aattatgtta caatatgcat taacaggaaa acaagtttta agactcaaag
     4981 gaggagatcc ttttattttt ggacgaggag gagaagaagc agaattttta aacgaaaatg
     5041 ctattgattt tgaaattatc cctggaatat cttctgcaat tggtgcagcc atttattccg
     5101 gtattcctct tacacataga aaattttcat catccgttgc aatagttaca ggtcatgagg
     5161 atcctacaaa agaagagaaa agtgttagat gggataaaat agcaacagct gttgatacca
     5221 tagtgattct tatgggagta gaaaagttga actctattct gaaaaagtta aaaaagcatg
     5281 gtcttccaga cgaaactaaa attgctgtaa ttgaaaaggg aaccctaaaa gaacaaaaaa
     5341 ttataattgg taatttcaat aatataaaaa agaaaatgaa aaatgcctct atacaacctc
     5401 cagccgtgat tattataggt gaagttgtat ctttatcaga gcgactaaga tggatttgat
     5461 ttattacgga tctatagatc ataactttaa gattattgca ataacaaaat ctgaaggttc
     5521 tatctctaag aaatttattg ataaaattga aaaaaatagc tctcgcctaa ttgtagtaaa
     5581 aacttcaaac atagtaagat ctgattctaa agtttttatc caaatttttc gcctgattgc
     5641 aaattggtct tctggatata ttattttttt gagctctaac gcagttgatg tattctttga
     5701 atttgctgag actttaaata taattagtga catagtttat gatttgaatt caaaatttaa
     5761 tgttgtggct attggcccat ctactagaac ttctcttaaa aaacatagaa tattagtgaa
     5821 ttcgatccct catgatcact cttctctagg aattatagat ttcttctccc aattgaaaaa
     5881 tagtaaatat gaatctaaga taataattcc gaggagtaag aaagcagata aatttctcaa
     5941 agagaaactc gtaaagctag gttttgtcgt taatgaattc tatatttata atgtaacacc
     6001 tgcaaaggtt gatccagtct ggatcgaatt tattgatctt cttaaaaaag aaaaaattga
     6061 ttctttaatt ttcactagtc catccaatgt taggtatttt ataaatatta taaagaaaca
     6121 ctcttccgat ttattacctt tagtttgtaa aataaaagtg atagtatcaa ttggaccgct
     6181 gactagtaaa gagctcacta aatataatat cccattttct gaatctacaa atcattcatt
     6241 agaaggagtt tatgaaaatc tttatatgaa ataataaaaa ttcaaataaa atttataaaa
     6301 ttttcatatt aatgtttttt ttaatagtat cgattgtaat atttatataa cataacgccg
     6361 ttatgaaata catgactaca gaagaactta acatggatta tagtaaatat gattttaagg
     6421 actctactga cttatatgta tatacaagta agaaaggtct ttcccgcggg actattgaag
     6481 aaataagtag aattaaaaat gaaccacaat ggatgcttga ttttagatta aggtcttatg
     6541 acatttttat gaataaacct atgcctgtat gggggggcga tttatcaaaa atcaattttc
     6601 aaaatatatt ttattataca aaagcatcag aaaagcaaag taaaagctgg gatgatgtgc
     6661 ctgactcagt tagaaaaaca tttgataaac ttggaatacc agaagccgaa aaaaaattcc
     6721 tagctggtgt tggcgctcaa tatgaatcgg aaactgtata tcataactta cgtgaagatt
     6781 tatctaaaca aggagtcata ttttcggata cagatacagc agttaaagaa catccagaga
     6841 ttttaaaaaa gtattttgga aaggttatac caccagaaga caataaattc gctgcattaa
     6901 atagtgcggt atggtcaggc ggttctttta tttacgttcc acctaatgta aaaattgacc
     6961 ttccattaca agcttatttt agaattaatg ctgaaaatat cggacaattt gaacgtactt
     7021 taattattgc tgacgaagga tcagaagtac actatattga aggttgtact gctcctgttt
     7081 atacaactga atccttgcat tctgctgttg ttgaattaat tgcaaagaaa gatgcaaaaa
     7141 taagatacac gactattcaa aattggagta aagatgtata taatttggta acaaaaagag
     7201 cgtatgccta tgaaaatgct agagtagaat ggattgacgg aaatattgga agtaaattga
     7261 ctatgaagta tcctggagtt tatatgcttg gaagaaatgc tcatgcagaa gttatatcga
     7321 tcgcctttgc tgggtcggat caacatcaag atgcaggagc aaaagcagtt catttagctc
     7381 ctgatactac atctagaatt acaagcaagt ctgttagtaa acaatccggt aggactacat
     7441 atcgtggttt attgcatgtt gcaaagggtg ctactggtgt caagtcaaac gttcgatgtg
     7501 atgcactttt acttgatgag acatcaagaa cagataccta tccgtatgtc gaagtgaacg
     7561 aagatgatgc aacgatttct catgaagcta gtgttggaaa aattggcgaa gatcaaattt
     7621 tctatcttat gagtagggga ttctcagagt ctgatgcctt gtcacttata gtgggcggat
     7681 ttatagaacc ctttacaaag gaattaccta tggaatatgc agtagaatta aatcgacttg
     7741 tcaaattaga aatggaagat tcagtaggtt agattcagta gtttagtaaa tacttacctt
     7801 tttttgatag tgataatctt aaaatgtacc aactatcttt gctagaaaaa aattttgaca
     7861 attttataga taactttaga gaaccaccat ggatgaaaca atccagatat gaggccttta
     7921 aaagttatac aagtttgcca ttagaatcct cggcacttta tagtaaatat tccaatgcaa
     7981 ataaattaat tgctgagaag gtttttttta atgatactaa cttacaaggt gaatcttcca
     8041 acgatctgga tattttttcg gatagaatgg atgagtttaa taaaactaca agtgtgttat
     8101 gtataaattc aaaaatccat aaatcatttg taacagatga gttagaaaaa aagggcatag
     8161 ttattttaaa tacttctcag gcgctaagag attactctga tataatacaa aaatatctga
     8221 ataaaaacaa actaaattac aacgaggaca aatttttggc cttatcttca gcagcatttc
     8281 atgatggact ttttatcttg attcctaaga atattgtatt ggaagatcct attagaatag
     8341 tgaattatct gtcagaggat ggtttatcat caatatctag aaacatagta gtatgtgaag
     8401 aattttcgaa agctacttta gttcaagaat tatattcaca acggtcaaag catccccaat
     8461 atcaacaaac ccatttcgaa ttgcttgaat cccatattct tcccaatgct caatgcgaga
     8521 tagttacatt acaatcaatg gattatgatg cggttagttt ttccaataga aaagcttatg
     8581 tagaaagaga tggaaagatg tcctggtcct tagctttatt tgggtcacaa ttgtcgaggt
     8641 ataaagttga tagcattatg caagggcaag gagctttagc agaagatgtc gaaattatat
     8701 ttggagtcgg taatcaatcc tttgacatta cgtccaattt aattcatgta gctcctaata
     8761 cacgaggtaa ggttttagtg aaatcagtaa tgaaagatac atcacagtct ctttttaaag
     8821 gaatgataaa aattcatact aatgcgaagg ctactgaatc gtatttagca ggacatgcta
     8881 tactattaga taaaggtgca aaatctgatg caattccagg cttagaaata gagtctaatg
     8941 aagttaaggc tacacattct gcttctgtag ctcagataga tgaaaaccaa atcttttacc
     9001 ttatgtctcg tggtctttca gaagaattag ctaaaagaga gatagtgggg ggatttttag
     9061 aacctatctc aagaaaaatg ggtccaacta tacgtgcttg gattaattat ttaattgaaa
     9121 ataaatggtt gggtaaaccg ttaaagttaa aaatagatga ggcaatggaa caaatccttg
     9181 aaattgaaaa atctagatat cgagaaacgg aagatatttt tgaaaaacac tataaatata
     9241 ggtaacatat attactctta tttttttgta gataaataat gtcagattgg gtaagagttt
     9301 gcgatctgga ttctctcaaa ccaggagagt tgatggattt tgatattgat gacaaaaaaa
     9361 ttcttcttgt taatataaag aataccatat atgctactga tagaatttgt acccatgctt
     9421 atgcagatct atctatgggt ttcttaaatg aagaagaaaa atctgtaaca tgtccacttc
     9481 atatgtctgc atttaatctt aatgatggaa gaccacttaa cttaccagcc gaagaaccat
     9541 taacggttta taaaactaag atagataaca attcagtctt tatacttgtc tgatttgtat
     9601 atttagagaa cgatgttgaa tgttttacta gaattcttaa tataatactt gtgcatccaa
     9661 agcaaacaga tggagataaa ttgtaaatag taatagtcat aataacaacc attatctcta
     9721 gtacctgata tcattagata tccattagat atcaataaga taaccaaatt ttttgaaaat
     9781 ttttatttaa atatttcatg aaaataaaaa tatttttcta tcgtaggtag taatgtcatt
     9841 ctattgaaaa ttggaattat tggattagga ttaatgggta gtaatctttg cttacgttta
     9901 attagaaaag gatttcaagt gaatgttcat aatcgcaatg tttctaaatc tctagcaata
     9961 gaaaaaaaag gtgctaaggt ttttaataca cccaaggagg tcgcagatta ttccgatttt
    10021 attataactt gtgtaacaaa ttttgaagca gtaaataaaa tatattttta taaaaatggt
    10081 gtaagtgaaa caaaaaataa aaatgtaatt gtttccgact ttagtaccct ttccccaaaa
    10141 gattcagttt attgttatca gaaattcaaa agaaaaaata taacaatgat gagtattcca
    10201 gtaatgggtg gccctaatgc cgcgttaacg ggtacactta tcccaattgt agcgggagaa
    10261 gaaatctcat ttaagaaaat aaagtatgta ttacaagaat taggtgatcc tattttttat
    10321 attggaaata aagcaggctc agcaagtgct atcaagttgg cgcttaatct caatattggc
    10381 ataatagcga tggctttatc tgaaggatta ttattagctg aaagatatgg aattgatcct
    10441 aacctttact tgaagatatt aaattgtact aattttaaaa ctggattgag tgaaaataaa
    10501 gggcctaaaa tgataaaagg tgattattct ccttcatttt atttgaaaaa tatgaaaaag
    10561 gatttagctc ttattatgga agcagcgaaa gaacaagacc tttctttacc tattacaggt
    10621 cttgtctttc aattctacaa ttatgcttca aaatcaatat ttgcagatct agattatagc
    10681 gtaatttaca aattcgtgag aaacttgcaa agtctagaat agattagtat gcagaatgta
    10741 aatattaatg cgaaatattt catcttatgt gctatgtaat cttagaatca ctaatttagt
    10801 ttaataagat tttaataaat ttaacaattt ttatttagta aaagatggat agtttagtag
    10861 atatatgtag atgttcaaga tgaaaaatat gaaatgagga aaatttatca ttgaataaat
    10921 taatcttaaa atattagtaa tagaataaaa aatttgatag taagtaaaaa tatttaacga
    10981 ggatatacac gatatttaac attgaattcc tgtaattaat atattcttaa gaaaacttat
    11041 ttagtataga aagatgagta cataaatagc ccttagatca gatttaattt ag
//
LOCUS       DATAFW010000031         5504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1419142, whole genome shotgun sequence.
ACCESSION   DATAFW010000031 DATAFW010000000
VERSION     DATAFW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419142"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VHJ57_00680"
     CDS             complement(<1..749)
                     /locus_tag="VHJ57_00680"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HEX2487081.1"
                     /translation="MPKIFADVSEKEISRSIAAMFMETMNEYTDSDVIIIGAGPAGLS
                     AGRDLGKLGIRTLIVEQNNYIGGGYWVGGYMMNPVTVRAPAQKIWDELGVPYRVIREG
                     LYATWGPHACSKLIAATCDAGVRFLQLTKFDDLVIKNKRVAGVVVNWMPVSALPRNIT
                     CVDPIAFESKIVIDASGHDSVAVKRLMDRGYVKWKGMDPMWVEGGEDAVVNYTGEVFP
                     GLIATGMSVTETHGLPRMGPTFGSMLLSGKKA"
     gene            complement(823..1959)
                     /locus_tag="VHJ57_00685"
     CDS             complement(823..1959)
                     /locus_tag="VHJ57_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX2487082.1"
                     /translation="MENFFGKKVTDKFNFQGQDKKPSELTHDNNINDIQKFDLYNKGI
                     NHMANEKLTDAIRCFELSLRIDPQFVNAWIKKGYTHFHLAEYSIAISCYDNALDLDIN
                     NFEAWNLKGLAYYRMNNLLKAIECCEKSIDINHNNGMAWYNLSCYLVLDGSVDDGMEA
                     LKRAIEIDISYAKRAVRDRDFENAKAEEGFRRIIEVVVLEAIRQGYDYTGKIIWITGM
                     DKVDVDDALLRLSMKGLVVRKEKKNFFNKEEYFELSKEISLKVGSFKRSGFMGKSREV
                     SPPVQQLKDISEILSKMRDSVEQGDVQATIGYFDKLINPSIHGSTMIEKFFNEHRDLR
                     LYQNRLKDKGQEYLNSHKSDFIKLMIEIDSKVRGTHFSNNVIDN"
     gene            2094..2519
                     /locus_tag="VHJ57_00690"
     CDS             2094..2519
                     /locus_tag="VHJ57_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX2487083.1"
                     /translation="MSDYYKILGVPKDSNQIQIRKAFRILALRYHPDKNKNSEESKQK
                     FIEIVKAYEVLSDEKVRQRYDSNTVRDENFQQFNWTPPADFVNFYSYEKLKREYKENQ
                     IIGGMWEISEKANAGLWKATFILLGSLGLISVFIILRIY"
     gene            2562..2903
                     /locus_tag="VHJ57_00695"
     CDS             2562..2903
                     /locus_tag="VHJ57_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX2487084.1"
                     /translation="MKKVFKGFKFTQNKKLKVLAIGNRIFITSFQMAGIEGIDVQTPK
                     EALSRINQTHSRNEVGLILLSDDIGKEIRLDIAKIRSVKPIPLIFELPSPGSKKEKVD
                     YRALLKQILGV"
     gene            2967..4307
                     /gene="thiD"
                     /locus_tag="VHJ57_00700"
     CDS             2967..4307
                     /gene="thiD"
                     /locus_tag="VHJ57_00700"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX2487085.1"
                     /translation="MNFRSVLSIAGSDSSSGAGIQADLKTFSSLGTYGCFAITAITVQ
                     NTKNIFDVFPIPSEIVIKQINSILHDIKIDAIKIGMVYDGEIINKIFQVLKNINIPII
                     VDPVFTSTSGTNLIKADAIKALTKKIIPIATIITPNINEAKQLSGVEIKTKEDLVRAI
                     NKIKKLGPKFVILKGGHLLSEHCIDTLIDDKLNIIEFSNPRLPIPENHGSGCNFSAAL
                     SAYISQGYEIPIACKFANEFINGILKNLLYLGKGYPVTDASITTSSSAFKFHVLEELS
                     RAIKELESLSDVGKLIPETQSNFAYAIPNATSIKEVAGIKGRIVRIDQKVKASSCIEF
                     GASQHIASAIISYMAINPLMRAGFNIKYDKKIIEVLQSFMKVSEYDRHLEPGKLKEKE
                     GFTIQWGIKKAIIKDKSVRGIYHKGDIGKEPMIIMFGHKPAEIIQYLTKIIDNY"
     gene            4346..5053
                     /locus_tag="VHJ57_00705"
     CDS             4346..5053
                     /locus_tag="VHJ57_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2487086.1"
                     /translation="MQAVILAGGLGTRLQPYTFFIPKPMLPLGNKPLLEYLIDWIKKL
                     KNIDEVIICVSYLHRTIEDYFEDGGRFGMKITYAHSERPLATGGQLKTAEKMLNDRFI
                     CLYGDSVYDFNLEKMVKGHEKSKAFLSMALMKYKTRLKYGFIDTNDKGNIVTAWREKP
                     EVEGLINIGCYVMEKEFLRMIPKSSTIGMDKIVKNVITQKKKVNAYTIASGFIDIGDK
                     RSYLDAYKKYMKKLGSI"
     gene            5149..>5504
                     /locus_tag="VHJ57_00710"
     CDS             5149..>5504
                     /locus_tag="VHJ57_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 family protein"
                     /protein_id="HEX2487087.1"
                     /translation="MFDINADKNLSADPSSDSNLKFYVRKMHYQGSLMINICDEELLG
                     TNIVSESLNINITNDFFNELANEKEISNLLKRCSIANLIGRRVVEKTLSLGLANRNSI
                     KIVSDIPFLMIFKFYN"
BASE COUNT         1977 a          901 c          840 g         1786 t
ORIGIN      
        1 gctttttttc cagataatag cattgaacca aaggtagggc ccattcttgg gagtccatga
       61 gtttcagtta ctgacatacc tgttgcgatg agaccaggga atacttcacc agtataattg
      121 actactgcgt cttctccacc ctctacccac ataggatcca ttcctttcca ctttacatag
      181 cctctatcca tcaatctctt tactgcaaca gaatcatgac ctgatgcatc aataacaatc
      241 ttgctttcga atgcaattgg atctacacaa gtgatatttc ttggtaaagc agaaacgggc
      301 atccagttaa ctaccacgcc tgcgacacgc ttatttttta ttaccaagtc atcaaattta
      361 gttaattgca aaaatctaac tcctgcatcg caagttgcgg ctatcaactt cgaacaagca
      421 tgaggacccc atgtagcgta tagcccttcc cttattactc tatatggaac tcccaattca
      481 tcccaaatct tttgtgctgg ggctcttaca gtaacaggat tcatcatata acctcctacc
      541 caataacctc ctccaatata gttattttgt tcaacaatta aggttctaat tcctaatttc
      601 cctaaatccc tacctgcaga taaaccagca ggaccagcac caataataat aacatcagag
      661 tctgtatatt cattcattgt ttccataaac attgcagcaa ttgaacgact aatttctttc
      721 tcgctgacat cagcaaaaat ttttggcata ttattatcac cataaacaca ttatatatta
      781 ttaatgcatt taatctctca agtcacataa acaaagataa ttttaattat ctattacgtt
      841 attagaaaaa tgggttcctc taaccttact gtcaatttct atcatcaact ttataaaatc
      901 agatttatga gaatttaaat actcttgtcc tttgtcttta agacgatttt gataaagtct
      961 taaatctcta tgttcattaa agaatttttc gatcattgta cttccatgaa tagaaggatt
     1021 gatcaattta tcaaaatatc ctatagttgc ttgtacatca ccttgttcta cagaatctct
     1081 cattttagat aaaatttcac tgatgtcttt taattgttga acaggaggtg acacttccct
     1141 agatttaccc atgaagcctg agcgtttgaa tgatcccacc tttaaagata tttctttaga
     1201 aagttcaaaa tattcttctt tattaaaaaa gtttttcttt tctttcctta caactaaacc
     1261 tttcatcgaa agtcttaaca aagcatcatc cacatcaact ttatccatcc cagtgatcca
     1321 aatgattttg cctgtataat cgtaaccctg tcttattgcc tctaacacta caacctcaat
     1381 tattctccta aatccttctt ctgcttttgc attttcaaaa tctctatctc tcacagcacg
     1441 tttagcataa gaaatatcaa tttcaatggc tctcttcagt gcctccattc catcatctac
     1501 actgccatca agaacgagat aacatgatag attataccat gccattccat tattgtgatt
     1561 aatatcgatt gatttttcac aacattctat tgcttttagc aagttgttca ttctataata
     1621 agcaagcccc tttaggttcc aagcctcaaa attgtttata tctaaatcta aggcgttatc
     1681 ataacaagaa atggctatac tgtattcggc aagatgaaaa tgtgtatacc ctttttttat
     1741 ccatgcatta acaaattgag gatcaattct taaagataat tcaaaacatc taatagcatc
     1801 ggtgagtttt tcattagcca tatgattaat tcccttatta taaagatcaa atttttgaat
     1861 atcattaata ttgttatcat gagttaattc agaaggtttt ttatcttgcc cttggaaatt
     1921 aaatttatca gttaccttct ttccaaagaa attttccata taataagaga tccattcacg
     1981 aagataaata gattatgatt tatttgttat agtgactaat atatctgatt gacttttaaa
     2041 aatataaaat tcaagaaaaa attattattt tagctgcccc attatactat taagtgtcag
     2101 attactataa aatattagga gtaccaaagg attctaatca aatccagata agaaaagcct
     2161 tccgcatctt agcgctacga taccatcctg ataagaataa aaattcggaa gaatcaaaac
     2221 aaaaattcat tgaaattgtt aaagcatatg aagttctctc agatgagaaa gtgagacaac
     2281 gatatgattc taatactgtc agggatgaaa actttcagca atttaattgg acacctcctg
     2341 cagattttgt taatttttat agttatgaaa agctcaaaag agaatacaag gagaatcaaa
     2401 taattggtgg tatgtgggag ataagtgaaa aggcaaatgc aggattatgg aaagcaacat
     2461 ttatcttgtt aggatctttg ggtctaatat ccgtctttat tatattaaga atttattaag
     2521 attgagacaa aattaattat ttcatacagt ttagaactta aatgaaaaaa gtttttaaag
     2581 gatttaaatt tactcaaaat aagaagttga aagttttagc aataggaaat cgtatcttta
     2641 taacaagttt ccaaatggct ggaatcgaag gaattgatgt tcaaactccg aaagaagccc
     2701 tttctcgcat aaaccaaacc cattctagaa atgaagtagg cttgatttta ttaagtgatg
     2761 acattgggaa agaaattaga ttagacattg ctaaaatacg ttccgtaaaa cctattcctt
     2821 taatatttga acttccttct ccaggcagca aaaaagaaaa ggttgattat agagcattgt
     2881 taaagcaaat acttggagtt taattatatt tttctttctc gcattaatta agttttagaa
     2941 cctgagtata gtatattcga tatagtatga atttccgttc agtgttaagt attgctggaa
     3001 gtgattcctc ctctggagcc ggcatacagg cagatctcaa aacattttcc tctcttggaa
     3061 cttatggatg ttttgccatt actgcaataa ctgttcaaaa tacaaagaat attttcgatg
     3121 tgtttcctat cccaagtgaa atagtaataa aacaaataaa ttccattcta catgacataa
     3181 aaatagatgc tattaaaatt ggcatggtct atgatggaga aataattaat aaaatatttc
     3241 aggttttaaa aaatataaat attccaatta tagtagatcc ggtatttact tctaccagcg
     3301 ggaccaattt aatcaaggcc gatgcaataa aagctttaac gaaaaaaata attcctattg
     3361 ctacaataat tactcccaat ataaatgagg ctaaacaact ttctggagta gaaattaaga
     3421 ctaaagaaga tttagtcagg gctataaata agattaagaa attagggccc aaatttgtaa
     3481 tactaaaggg ggggcatctc ctctcagaac attgtattga cacgttaatt gatgataaac
     3541 ttaatataat tgaattctcc aatcctcggc tacctatccc agaaaaccat ggttctgggt
     3601 gcaatttctc tgcagcctta tcagcataca tttcgcaagg gtatgaaata cctatagcat
     3661 gtaaatttgc taatgaattt ataaatggta ttttgaaaaa tcttttgtat ctcggaaaag
     3721 gatacccggt aactgatgct agtataacca cctcttcttc agcctttaaa tttcatgtat
     3781 tagaagaact atcgagggct attaaagagt tagaatctct atcagacgta ggcaagttaa
     3841 tccccgaaac acagtctaat tttgcatatg caataccaaa tgccactagt atcaaggaag
     3901 ttgctggaat aaaaggaaga atagtaagga tagaccaaaa agtgaaagca agttcgtgta
     3961 ttgaatttgg tgcatcacaa catattgcat cagcaatcat atcatacatg gctatcaatc
     4021 ctttaatgag agctggattt aatataaaat acgataaaaa aataatagaa gtattacaat
     4081 cctttatgaa agtatctgaa tatgatagac atttggaacc tggaaaatta aaggaaaagg
     4141 aaggttttac aatacaatgg ggaattaaga aagcaataat aaaagataag tcggtaaggg
     4201 gaatttacca taaaggagac ataggtaaag agcctatgat aataatgttt ggacacaagc
     4261 cagcagaaat tattcaatat ttgacaaaaa taatagataa ttactaatct aaaagtatat
     4321 tacattattc acttaaacat tgtagatgca agcagttata ctagctggag gccttggcac
     4381 cagactacaa ccttatactt tctttatacc aaaacctatg ttaccattag gaaataagcc
     4441 attacttgaa tatttaatag attggataaa aaaattgaaa aatatagatg aagtaattat
     4501 ttgtgtaagt tatctgcatc gaaccatcga ggattatttt gaagatggtg gaagattcgg
     4561 aatgaaaatt acttatgccc attctgaacg acctttagcc actggagggc aattgaaaac
     4621 tgcagaaaaa atgttaaatg ataggtttat ttgtctttac ggtgattcgg tgtatgattt
     4681 taatttagaa aaaatggtca agggacatga aaaatcaaag gcttttcttt ctatggccct
     4741 aatgaaatac aaaacaagat taaagtacgg ttttatagat acaaatgata aaggaaatat
     4801 tgttacagct tggagagaaa aacctgaagt cgaaggttta ataaatattg gatgttatgt
     4861 aatggaaaaa gaatttttaa gaatgatacc aaaatctagt actatcggaa tggacaaaat
     4921 tgtaaaaaat gtaattacac aaaaaaaaaa agttaacgct tatacaatag catctggatt
     4981 tattgatatt ggagataaaa gatcctattt agacgcttat aaaaaataca tgaaaaaatt
     5041 aggtagtatt tagcaacatt tattataagc gtaagtgcta tgttttaaat taattactaa
     5101 ttagtagaac gtgatctttt tatgcttacc caataaaagt gttcatatat gtttgatatc
     5161 aatgctgata aaaacctctc agctgatcca tcaagtgatt ctaatctgaa gttctacgtt
     5221 agaaaaatgc attatcaagg atcactaatg ataaatattt gtgatgagga gttattaggt
     5281 actaatatag tgagtgaatc attaaacatt aacataacaa atgatttttt caacgagctt
     5341 gcaaatgaga aagagatatc taacctatta aaaagatgtt cgatagctaa tttaattgga
     5401 agaagagttg tagaaaaaac cttgagtttg ggtcttgcaa acagaaatag tataaaaatt
     5461 gtttcagata ttccttttct aatgatattt aaattttata acaa
//
LOCUS       DATAFW010000032         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4943301, whole genome shotgun sequence.
ACCESSION   DATAFW010000032 DATAFW010000000
VERSION     DATAFW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943301"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="VHJ57_00715"
     CDS             complement(<1..1414)
                     /locus_tag="VHJ57_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX2487088.1"
                     /translation="MTNKNSSKIKLIKIIIKLLPIIINFKRDRKEWVKREGKNVDENK
                     YRKHALQALKIFIQLGPSYIKLGQWLSSRSDILPQPYLEVFATLQDDVPVASFEKVKK
                     IIEEELGNLEEKFDYFDKEAFSGASLGQVYHARYNGREVVVKISRPGIERIVEEDISI
                     IKKLIPLSTRFIDPNLRFSVEGMFSQFTETIYEEMDYIKEAENLLAIKKNLAKEKKII
                     IPNVILDRTSKHIITMEYEPGIKITDVETLDKKGIDRQKLVILIHHIFFKMLLRNNIF
                     HSDPHPGNIAVKDDGSIILYDFGMVGRLDNETRLLLIRLYLGLIDKDPVRTVDVLYQL
                     GTLDYTTNRNIVEKAIELSIRSMHGKHVDKMEVKSLMDLSNKTLSKFPFRLPKNLALY
                     MRMTSIVEGIYHQHKIRFQFVKVLSNLFEQEGLINDAYIEEIKYSFQKFIKNIEASVN
                     LSQTIKTYLDNQETNIKKTDK"
     gene            complement(1451..1876)
                     /locus_tag="VHJ57_00720"
     CDS             complement(1451..1876)
                     /locus_tag="VHJ57_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487089.1"
                     /translation="MNYIDGDGNILIPKSVRPIIDYTETNLGSKKGAKKQYRYENLHI
                     REYEDYYSVHLDKVDPRIDPLGHLLKDAPEYLTCWMIASTIGLKVGTTFYKKRKEKDN
                     GNQLKALPLGIITGILAGTTTYMTTYHLHNFIRKAKLRK"
     gene            2019..2555
                     /locus_tag="VHJ57_00725"
     CDS             2019..2555
                     /locus_tag="VHJ57_00725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2487090.1"
                     /translation="MNKVKLLHSKRVYTGKISIRKDEFILQDKKIQKEIVEHSPSVGL
                     VPIIDYSNILLITQYRHPAKRTLLEIPAGKIEDGETKEQAARRELYEETGYAGTLSFL
                     TKWYLAPSYDTELMYIFLVTNLKKMNYKQGKDDDENIKLKKLSLKTALKKCINGEIID
                     CKTVAALLAYQEYSKPVI"
     gene            complement(2646..2984)
                     /locus_tag="VHJ57_00730"
     CDS             complement(2646..2984)
                     /locus_tag="VHJ57_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HEX2487091.1"
                     /translation="MMRGGNREMRRMLGKMGLDMNEMEGIEEVIIKTETKEMYLIKPQ
                     VVEMKGKDNTIFQIIATDIEEKQRETPSLKEEDIILVMQQANVSKEKAIQALTDSKGD
                     IAQAILTLTT"
     gene            complement(3005..>3490)
                     /locus_tag="VHJ57_00735"
     CDS             complement(3005..>3490)
                     /locus_tag="VHJ57_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HEX2487092.1"
                     /translation="WKLKPLPLNPRNKLINHISVLFDTNVNEEFPDIQCEFSKRTGRI
                     KNFSIESNLFATLRKDGGLALTIFGAKMMIKYTQFIENCIIADDDVIPFISEGRSLFC
                     KHVKWCGKNVNSGSDVVVIDSKHEVLAVGKAICGNKIMKKFDFGVAVKIREGIKSRNN
                     Q"
BASE COUNT         1141 a          551 c          428 g         1370 t
ORIGIN      
        1 gcttatctgt ttttttgata tttgtttctt gattatctaa atatgttttt atagtttgag
       61 ataaatttac tgatgcttct atatttttta taaatttctg aaaagaatat tttatttctt
      121 caatgtatgc atcattgatt aatccttcct gttcgaataa attcgataga acttttacaa
      181 attgaaatct tattttatgt tgatgataaa tcccttccac aattgaggtc atcctcatat
      241 ataatgctaa atttttgggt agtctaaatg gaaatttact taaggtttta tttgataaat
      301 ccattaaaga tttcacttcc attttatcta cgtgttttcc atgcatcgat ctaatactta
      361 attcaatagc tttctcaaca atatttctat tagtggtata atctaatgtt ccgagttgat
      421 ataatacatc aaccgttctt acaggatctt tatcaatcaa acctagatac agtctaatca
      481 acagtaatct agtttcatta tcaagtcttc caaccattcc aaaatcgtag agaattattg
      541 aaccatcatc tttgacagct atatttccag gatgaggatc agaatgaaaa atattattac
      601 gtaatagcat cttgaaaaat atatggtgta ttaatattac taatttttgt ctatcaatac
      661 cttttttatc taatgtttct acatctgtta tctttattcc gggttcatat tccatagtta
      721 taatatgttt agaagttcta tcaagtatta cattagggat tataattttt ttctctttag
      781 ctaggttttt cttgattgct aatagattct ctgcttcttt tatgtaatcc atttcttcat
      841 aaatagtttc agtaaattga gaaaacattc cttcaactga aaatcttaaa tttggatcta
      901 taaatctagt agataacggg atcaattttt ttattattga aatatcttct tctacaattc
      961 tttcaattcc tggtcttgaa atctttacta ctacctctct tccattatat cttgcatgat
     1021 atacttgtcc taaactagcg ccagaaaaag cctctttatc aaaataatca aacttttctt
     1081 ccaaatttcc taactcctcc tcgattattt tttttacttt ttcaaaagat gctactggaa
     1141 catcatcttg taaagtagca aatacttcta aatagggttg tggtaatata tcagaacgag
     1201 aagatagcca ttgacctaat tttatatacg atggtcccaa ttgaataaaa atttttaagg
     1261 cttgtagtgc atgttttcta tatttatttt catcgacatt ttttccttca cgttttaccc
     1321 attcttttct gtctctctta aaattgatta ttattggtag taacttaatt atgattttta
     1381 ttaatttgat ttttgaggaa ttcttgtttg tcatttcaga aatatatatt taaacaataa
     1441 tcaaaatttt ttattttctt aattttgctt ttctaataaa attatgcaaa tgatatgtag
     1501 tcatatatgt tgtagttcca gctaaaatac ccgtaataat tcctaatggc aatgctttta
     1561 attgattacc attatctttt tcttttcgtt ttttataaaa tgttgtacct acttttagac
     1621 caatagtact agcgatcatc cagcatgtaa ggtattccgg agcatctttt aagagatgtc
     1681 caaggggatc gattcttgga tcgactttat ccagatgtac gctataataa tcctcatatt
     1741 ctctgatatg taaattctca tatctatatt gttttttcgc tcctttttta cttcctaaat
     1801 tcgtttcagt ataatcaatt ataggcctga cggatttagg aataagaata ttgccatcac
     1861 cgtcaatata gttcatctaa tattagaatt aaacaaatat catataaaat taactttata
     1921 ttttatttat tgaatatagg ttaatataaa accctgatag tataggaaag tatattttac
     1981 tttgaagaaa aaatattctc aatttatagt tgaatcaaat gaataaagtt aaattattac
     2041 atagtaagag agtctataca ggaaagatat caattagaaa agatgaattt attttacagg
     2101 acaaaaaaat tcagaaggag attgttgaac attctccatc agtaggactt gttcctataa
     2161 tcgattattc aaatattctt ctaattactc agtatcgaca tccagcaaaa agaactctat
     2221 tagaaatacc tgctggaaaa atagaagatg gagaaactaa agagcaggct gctagaagag
     2281 aattatatga agagactggt tacgcaggaa cattatcttt tttgactaaa tggtatttag
     2341 caccaagcta cgatacagaa ctaatgtaca tttttttagt aacgaattta aaaaaaatga
     2401 actataaaca aggtaaggat gatgatgaaa atataaaatt aaaaaaacta agtctaaaaa
     2461 ctgcattaaa aaaatgcatc aatggtgaaa ttatagattg taaaacggtt gcagcattac
     2521 ttgcttatca agaatatagc aaaccagtga tataaaaata atacctataa aatgttatat
     2581 aatagtacaa attcaaataa ttctataata tattgatgaa taaaataaaa tataattatt
     2641 tataatcatg tagttagagt taagatagct tgtgctatgt cacctttaga atcagtcaag
     2701 gcttgaatag ctttttcttt tgatacatta gcttgctgca tcactaatat tatgtcctct
     2761 tcttttaaag atggagtctc tctttgtttt tcttctatgt ctgtagcaat aatttggaaa
     2821 attgtattat cttttccttt catttcaacg acttgcggtt tgattagata catttcttta
     2881 gtctcagttt ttattattac ctcctcaatt ccttccatct cattcatatc tagacccatt
     2941 tttccaagca ttcttctcat ttctctattt cccccacgca tcattatcta tattacctct
     3001 aagatcattg attgtttcta ctttttattc cttctctaat ttttacggct acaccaaaat
     3061 caaatttttt cattatcttg tttccacata tcgcttttcc cacagccaat acttcatgtt
     3121 tactatcaat gactactacg tctgatccac tattgacatt tttcccacac cacttcacat
     3181 gtttacaaaa taatgatctt ccttcactaa tgaatggaat tacgtcatca tctgcaataa
     3241 tacaattctc tatgaattgt gtatatttta tcatcatttt tgcgccaaat atagttaaag
     3301 caagtcctcc atctttacgt aaagttgcaa ataaatttga ttctatagaa aaatttttaa
     3361 ttctgcccgt tctctttgaa aattcgcatt gtatatctgg gaattcttca tttacatttg
     3421 tatcaaaaag aacagatata tgattaatca gtttatttct agggtttaga ggtaaaggtt
     3481 tcaatttcca
//
LOCUS       DATAFW010000033         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_715309, whole genome shotgun sequence.
ACCESSION   DATAFW010000033 DATAFW010000000
VERSION     DATAFW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715309"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..249
                     /locus_tag="VHJ57_00740"
     CDS             34..249
                     /locus_tag="VHJ57_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HEX2487093.1"
                     /translation="MSKTIEEKVVPAEVIQIVGRTGIAGEVIQVRVRVLEGKDKGRIL
                     TRNVKGSVRMNDVLMLRETEREARKIK"
     gene            296..511
                     /locus_tag="VHJ57_00745"
     CDS             296..511
                     /locus_tag="VHJ57_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HEX2487094.1"
                     /translation="MSKTATSLRNCFFCGQRISSASGIMLVKNDGSIQWTCSNKCKKN
                     LRKLKRDPRKMKWTNKYVKGGIKVSKS"
     gene            528..929
                     /gene="ndk"
                     /locus_tag="VHJ57_00750"
     CDS             528..929
                     /gene="ndk"
                     /locus_tag="VHJ57_00750"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX2487095.1"
                     /translation="MSEQTLIIIKPDAFERKLIGIILSRFENKGFLIKELKSYIFAKE
                     KAEEFYSDHKNKPFFSELVSFISSSTVVACILEGDNAINIVRQMIGSTRSFEAQPGTI
                     RGDYGLGITNNIIHASDSDESYIKESKVIFT"
     gene            937..2766
                     /gene="infB"
                     /locus_tag="VHJ57_00755"
     CDS             937..2766
                     /gene="infB"
                     /locus_tag="VHJ57_00755"
                     /inference="COORDINATES: protein motif:HMM:NF003078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX2487096.1"
                     /translation="MNLRQPVVVVLGHVDSGKTSLLDKIRGTGVQAREAGGITQHIGA
                     SFFPIETIKTITGPLYERLSKTDEQIPGLLIIDTPGHEVFANLRSRGGSAADIAIVVA
                     DVNKGFEPQTIESIDILKKRKVPFVVALNKVDMVSGWKGKKKGETSQSRKLNTINLGF
                     ITEEIKKQDSNVLTLLDEKIYSVVGSLSRMGFNSEAFWRIKEFDKELAIVPISAVSGI
                     GIPELMAVLVGLAQQYMKKRLERRYDYVRGIILEINEELGLGPSANMILLDGTIKQGD
                     TIMVAKRDEIVVTKVKALLLPKPLDEMRDPRDKFRSVIEVISAAGLRIVSPDLEGVLA
                     GSPLYVVRHKEEEKEIRHFIELEVKDTFINNTNSLGVILRCDTIGSLEAIIDMLKKEN
                     IPIQSADIGHITRRDVLAAHAVKDKDRHLGVILGFNVKILDEAKKEAKEQEVQIFNEK
                     VIYNLVRNYTDWVKYQKEHADSILFEEIPPICKIQFLKGFVFRRNDPAVFGAEILIGK
                     LRQKNHVINEDGSKVGIIHQIQDKGKNIEEAKKGMQVAVSIRGPTIGRQINEGDFFYS
                     DLNSRQAKLLIEKFGHRLNEEEKEIFEFILNMKRKSDPAFGYI"
     gene            complement(3000..3347)
                     /gene="trxA"
                     /locus_tag="VHJ57_00760"
     CDS             complement(3000..3347)
                     /gene="trxA"
                     /locus_tag="VHJ57_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX2487097.1"
                     /translation="MHTNTENNDGDLIIELSDENFEKSINGKKAILVDFWATWCGPCK
                     FMLPVFNKLAKKYASKIDFGRLNVDDSQSIAVKYDVYAIPTFIVFYKGKIVERAVGAV
                     GEKGLENIIGQLP"
BASE COUNT         1300 a          483 c          678 g         1039 t
ORIGIN      
        1 aataataata ataatagtca ggtgttataa ttaatgagta aaacaataga agagaaagtt
       61 gtacctgctg aagtaattca aatcgttggc agaacaggta ttgcaggaga agtaatccag
      121 gtacgagtca gagttttaga aggaaaagat aaaggcagaa ttttaaccag aaatgtaaag
      181 ggttctgttc gaatgaatga cgtattgatg ctacgagaga ctgaaagaga agcgagaaaa
      241 ataaagtaat aataaataat atataaaaag aaaaaaatat gaggtttaat taaaaatgag
      301 taaaacagct acatctttac gcaattgttt cttttgtgga caacgtattt cttctgctag
      361 tggaattatg ttggtcaaga atgatggatc tattcaatgg acttgttcca acaaatgcaa
      421 aaaaaatctt cgcaaattaa aaagagatcc gcgaaagatg aaatggacta acaaatacgt
      481 taagggtgga atcaaagtct ctaaatcata aaatagaatt gatatagatg agtgagcaaa
      541 cattaataat tataaaacca gatgcctttg aacgcaaact catcggaata attttgtcca
      601 gatttgagaa caagggattt ctgattaaag aactcaaatc ctatattttt gcaaaagaaa
      661 aagcagaaga gttttattcc gaccacaaaa ataaaccatt tttcagtgaa cttgtgtcgt
      721 ttatctcctc ttctaccgtt gttgcctgta ttttagaagg agataatgca ataaatattg
      781 taagacagat gattggctcg actaggtctt ttgaagccca gccaggcaca attagaggcg
      841 attatggtct tggcattaca aataatatta ttcatgcctc cgactcagat gaaagttata
      901 taaaagaatc taaagtaatt tttacttagt atcaacgtga atctccgaca gcctgtagtt
      961 gttgtattgg gtcatgtcga ctcggggaaa acttccttat tagataagat tagaggcaca
     1021 ggagtgcaag cacgtgaagc tggaggtatt acccaacata ttggggcaag cttttttccg
     1081 atagagacaa ttaaaaccat aacaggacct ttgtatgaaa gactcagcaa aacagatgag
     1141 caaattcctg ggttattaat tattgatact cctgggcatg aagtatttgc gaatcttaga
     1201 agtcgaggag gatctgcagc agatatagca attgtagttg ccgatgtaaa caaaggtttt
     1261 gaaccacaaa cgatagaaag tatagatata ctaaagaaga gaaaggtccc ttttgttgta
     1321 gcattaaata aagtggatat ggtttcaggt tggaaaggta aaaaaaaggg tgaaacaagt
     1381 caaagtcgaa agctcaatac gattaattta ggctttataa ctgaagaaat taaaaaacag
     1441 gattctaatg ttcttactct tctagatgaa aaaatttata gtgttgttgg atcactgtca
     1501 cgaatgggct ttaattctga agccttttgg aggattaaag aatttgataa agagttagca
     1561 atcgtaccaa ttagtgctgt ttctggaatt gggataccgg aattgatggc tgtccttgta
     1621 gggttagctc aacagtatat gaaaaaacgg ttagagagac gttatgacta cgttagaggt
     1681 ataattctgg aaatcaatga agaacttggc ctaggtcctt ctgctaatat gatactatta
     1741 gatggtacta ttaagcaagg agatacaatc atggttgcaa agcgagatga aatagtagtg
     1801 actaaagtta aagccttact tcttccaaag cctttagacg aaatgcgtga tccacgtgat
     1861 aaatttaggt cagtaattga agtaatctca gcagcgggtt taagaatagt ctctcctgat
     1921 ttagaaggtg tcttggcagg aagcccattg tatgttgtaa gacataaaga agaagaaaaa
     1981 gaaattcggc attttattga attagaggtc aaagatactt ttatcaataa tactaattcg
     2041 ttaggagtga ttcttaggtg cgatacaatt ggttccctag aagctataat tgatatgttg
     2101 aaaaaagaaa atatacccat tcaaagtgct gatattggcc atataaccag aagagatgtt
     2161 ctggcagcac atgccgttaa agataaagat agacatcttg gagtgatatt aggttttaat
     2221 gttaaaattc tagatgaagc gaaaaaggaa gcaaaagaac aagaagttca aatatttaat
     2281 gaaaaagtaa tttataatct tgtaagaaac tatactgact gggtaaaata tcaaaaggaa
     2341 catgctgact ctattctatt tgaagaaatt ccacctattt gtaaaataca gtttttaaaa
     2401 ggatttgtat ttagaaggaa tgacccagcc gttttcggag cagaaatact tataggaaaa
     2461 ctcagacaaa aaaatcatgt tataaatgaa gatggatcta aagttggaat cattcatcaa
     2521 attcaagata aaggaaaaaa tattgaagaa gcaaaaaaag gtatgcaggt tgctgtatct
     2581 ataagaggtc caactatagg gagacaaatc aatgagggag atttctttta ttctgatcta
     2641 aatagtaggc aggcaaagct attaattgaa aaatttgggc ataggcttaa tgaagaagaa
     2701 aaagaaatat ttgaatttat attaaatatg aaaagaaaat ctgatccagc atttggttat
     2761 atctgatctg taggtaggaa aataaacgat ttggacgatg aatttcaaat aaaaaacaat
     2821 tataaaaata aaaataatca tatacataac aaaatattat cccacttcga aatgaaaatg
     2881 aattaataca gattattatt cttgcctaga taattcttca gcagcagcag cagcagtagt
     2941 agtagtagtc agtactggca ttaggtaaat ttgtttaact atacattaaa acctaagatt
     3001 tagggaagtt gtccaatgat attttctaat cccttttctc caacagcacc tactgcccgc
     3061 tctacgattt ttcctttata gaatacaata aaggttggga ttgcatagac atcgtatttt
     3121 acagcaatag attgcgagtc atctacattc agccttccaa aatcaatttt tgacgcatat
     3181 ttttttgcca atttgttgaa aacaggtaac ataaatttac atggaccaca ccatgtggcc
     3241 caaaaatcaa caaggatagc tttcttacca tttattgatt tttcaaagtt ttcatcagaa
     3301 agttcaatta tcagatcgcc atcattattt tctgtattgg tgtgcatgga gcgttcatca
     3361 taccttaaga tgataaaatt atataaaatt attggagaaa gatagataaa attagtatca
     3421 tagatctact aagaatcttg cagtataata accggtttgg gttcgtttta tttccatttc
     3481 gtgatacgtt atagacttta
//
LOCUS       DATAFW010000034        10399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2477845, whole genome shotgun sequence.
ACCESSION   DATAFW010000034 DATAFW010000000
VERSION     DATAFW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2477845"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VHJ57_00765"
     CDS             complement(<1..382)
                     /locus_tag="VHJ57_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487098.1"
                     /translation="MTTATMDSKLSSIVITSILAFAIIAGVPALFENVLAQQDGSGQS
                     GGDAGMDQSQSGGDAGMDQSQSGGDAGMDQSQSAVDAGMAQTQGNDTGFAQSQPGSDM
                     VGTTQDGDEEDGGGDGDGDGDGDGD"
     gene            882..1139
                     /locus_tag="VHJ57_00770"
     CDS             882..1139
                     /locus_tag="VHJ57_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487099.1"
                     /translation="MIDYKRFRDIRNSPSNSMNFGFETYSLADKVIQQQHDLLNDIGM
                     SSLTFPFNQSKIGEPESMVKEDINRKIRSLSKNSDDFYNID"
     gene            2096..2290
                     /locus_tag="VHJ57_00775"
     CDS             2096..2290
                     /locus_tag="VHJ57_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487100.1"
                     /translation="MYRIVGMPYRIRISNGIEEIEMESDQISDIDTMIEKVEQLKQLF
                     REKPSIDSSPTKEKSYLGKS"
     gene            complement(2405..2920)
                     /gene="nuoB"
                     /locus_tag="VHJ57_00780"
     CDS             complement(2405..2920)
                     /gene="nuoB"
                     /locus_tag="VHJ57_00780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX2487101.1"
                     /translation="MIKDLINPANFNLLVSQVSDVLVGALDQPLGYAINWGRIWSLWP
                     VHLETACCSVEFGATSSPRYDCERFGIIEAFGSLRQCDLIVVQGTVTRKMAPRLRMVY
                     DQMADPKYVIAMGACSITGGLYIDSYNVLPGIDGIIPVDVYVPGCPPRPETLIQGCML
                     LQEKIKRSKIK"
     gene            complement(2990..3364)
                     /locus_tag="VHJ57_00785"
     CDS             complement(2990..3364)
                     /locus_tag="VHJ57_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX2487102.1"
                     /translation="MAGELASEFTPILYAFGIGIMAGIPSLILARLLSPRKRYNPVKF
                     LPMECGQVPTGEGRSHFMMQYYAYILMFVVFDVMSIFLYAWGSSLLSIPKEATLPMMA
                     FLAIMFAAMAYALYLSGRKGIW"
     gene            complement(3400..4608)
                     /locus_tag="VHJ57_00790"
     CDS             complement(3400..4608)
                     /locus_tag="VHJ57_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2487103.1"
                     /translation="MEMYNYDIAIVGGGPAGLSAAYTAAKSGARVILFEREKSIGQYV
                     RTSGVSWIGEMKKLEIPEEIYNPIKNYRFVSPNNEITITGTEYRSCVLDVRSLYQYLS
                     FLAIKAGAEISINSNVIDVLCIKNNNKISGLIVNTPKGKVELRTSLVIDASGFNSFIG
                     RKLGYVSTWKRYGVGAEYECYCDQIDHETWTLMVGSIYSPAGYAWLFPLSPNRVRIGV
                     GIGKPESMLDPLKLLNQIIEKKTKPLMELGNIQPIEYHYGYIPNEGARKCSVFDGLIL
                     VGDSAGQSNPLVLEGIRHAIEFGRLAGQIGSESLISSCSKNSLIHYENEWKKQLSSKI
                     ESALRVQSRWIGLTDDEWDHEISILRHMSVDEFIDFISSEFSPRKMIKLALNHPKLIA
                     RQLFNLIIKK"
     gene            4712..5191
                     /gene="bcp"
                     /locus_tag="VHJ57_00795"
     CDS             4712..5191
                     /gene="bcp"
                     /locus_tag="VHJ57_00795"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX2487104.1"
                     /translation="MSQKFHLREGNMAPDFIFKDSDNNDLRLSNFLGKRIVIYFYPND
                     FTPGCSTQASEFSIHFNKYLENNIIILGISPNDETTHQKFKEKMRIPFLLISDSNNKI
                     SQKYGVYTLKKFMGKEYMGVMRTTFLIDKHGKIVKIFYKVKPKGHSAEVLNYFLRDN"
     gene            complement(5218..5691)
                     /locus_tag="VHJ57_00800"
     CDS             complement(5218..5691)
                     /locus_tag="VHJ57_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487105.1"
                     /translation="MVKDGITSPYFEIETEPGFFTKTKYDIISLKITFQQIGESMSIL
                     KNFDYYQYLMCTMLPHIADTNPYKKVFQKLRFMIICSFTKFISLLHIRDYETIKKWNI
                     LSKKTLELVAEIVNGYRQGVEFREISNSINGDNEFCIFFNLDIEKMDEELNNYYL"
     gene            complement(5803..6231)
                     /locus_tag="VHJ57_00805"
     CDS             complement(5803..6231)
                     /locus_tag="VHJ57_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487106.1"
                     /translation="MVQDRGKMEKQQRLYELLKQQHEAEKDEILHYSTLLKEIENNLI
                     RNYINTLLSDGIKHIQYITQAMAKIEGASGSIHLTEEGINHSIEEEEDSKNILLECIG
                     LAQDKDTKELLQSVVVDEEHHIKILEHIRKLVQSYSKKIK"
     gene            complement(6484..6568)
                     /locus_tag="VHJ57_00810"
     tRNA            complement(6484..6568)
                     /locus_tag="VHJ57_00810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6530..6532),aa:Leu,seq:gag)
     gene            6673..6906
                     /locus_tag="VHJ57_00815"
     CDS             6673..6906
                     /locus_tag="VHJ57_00815"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HEX2487107.1"
                     /translation="MLIPVRCFTCGGLIADKYFEYKERVKSGEGDAEVLDSLGIKRYC
                     CRRMFISSVETIYQVIPYYEVLRRRMSEMQSES"
     gene            6919..8166
                     /locus_tag="VHJ57_00820"
     CDS             6919..8166
                     /locus_tag="VHJ57_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HEX2487108.1"
                     /translation="MPIITDIISRLIFNSRGSKTIEVDIISDDKFLGRASSPSGASVG
                     KHEAQSFKENSPEKSLEELNNNLSKFIGIESHDTRAVYDVLKNIDNSENYGKIGGSVA
                     YAISLASVESASKALNVPLFRLLKPVGDYKFPYPLGNILGGGSHAGPGTPDIQEYLIC
                     PIGASNIIEAIEINIRVHKQLKTILEKLDNNFTYGRGDEGAWAPNLNNDQAIEVVEQA
                     ILDSGYNSGKEVAIGIDFASSSFWNETTQSYDYLRQGLVRSTNEQIDFVNDLIDKYHL
                     IYVEDPVHEEDFEGMSIINKNHPECLITGDDMLVTNKKRVKESIKYNSCSGAILKVNQ
                     AGSLYEALEFVNECNKNDIKLITSHRSGESIDSHIAEIAVATSSKLLKTGVIGGERVA
                     KLNQLVRLAEYDLIKGMSDVFTS"
     gene            8189..8986
                     /gene="rpsB"
                     /locus_tag="VHJ57_00825"
     CDS             8189..8986
                     /gene="rpsB"
                     /locus_tag="VHJ57_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX2487109.1"
                     /translation="MNSDLITSDTSNAETNEDKELPEAENLPEAENLPEAENQSTNSL
                     NEVENSLVEKHDDEINIENSDSENLEKMILSTGIRVGTPVKTKSMSRYIVRANPEGLY
                     ILDISKTLYRIDVAAKFIGRTNIAKVAITSAREYGKKPVEKFCEATGATPILGRFMPG
                     TFTNPSLPKYMEPEIVIVTDPQADQQAVIEATRAGVPVIAISNSDNVISKVDLVIPAN
                     NRGRKALATVYWLLAREVMKKQGKIKSDKEMKVTIDDFETKLVEEIV"
     gene            8983..9828
                     /gene="amrB"
                     /locus_tag="VHJ57_00830"
     CDS             8983..9828
                     /gene="amrB"
                     /locus_tag="VHJ57_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEX2487110.1"
                     /translation="MRDLICRSPNYAGSYYPKDKGDLIKLLHFCFNESIFAPKKKQTL
                     NTKIFGILVPHGAYIYSGAISANAFYEIASQNIDTFILIGPDHKGIGKRISVTSEGYW
                     QTPLGDVLLDYTIINDLIKSCDFIEEDKITHQIEHSLEIQVPFIQYSCGNSFTIVPLL
                     LRDQDIETSVLLGNLIAKFSARKNAIIIASSCLTHYESNSNVQKKDCQMIKCIENLDI
                     ESFYQLIMNSNTSVCGYGAIASLMRAVKISGSQKGVLLRYATSGDVTSDIKNTVVGYA
                     SIAFV"
     gene            9840..>10399
                     /locus_tag="VHJ57_00835"
     CDS             9840..>10399
                     /locus_tag="VHJ57_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487111.1"
                     /translation="MTSTLASAPAKIILFGEHFVVYNQPIILASINKRMKIECKIEED
                     KNNTVNIETNHFGKKTFPLSIIESNNHSIVNDFFYPIIYILRKILRYYSEPYGITIKI
                     DSEIPYGVGLGSSAALSVAGVAAISGLNNIVKEKNEILNIAIETERIIHKNSSGADCV
                     VSTYGGLLYYQRTTSINMRSLNLNNEL"
BASE COUNT         3570 a         1638 c         1663 g         3528 t
ORIGIN      
        1 cgtcgccgtc gccgtcgccg tcgccgtcgc cgtcgccgcc accgtcttct tcatctccat
       61 cttgtgtcgt tcctaccata tctgatcctg gttgactttg tgcaaatccg gtgtcatttc
      121 cttgagtttg ggccattcca gcatcaactg ctgattgact ttggtccatt cctgcgtcac
      181 cgcctgattg actttggtcc attcctgcgt caccgcctga ttgactttgg tccattcctg
      241 cgtcaccgcc tgattgacca ctaccgtcct gttgtgctaa tacattttcg aataaagctg
      301 gcactcctgc aattatagca aatgcaagaa tggatgtaat tactatggaa gaaagttttg
      361 aatccatagt ggctgttgtc aattatagta tttatatatt caacaaatta tcttctataa
      421 catttttatc catttcatag tgagttattt acaatatttt cgtcaaataa ttttaactaa
      481 attacatctt gtataaatgg tttataaaag tacataactg aacgaactat ctaatttatt
      541 tttctttaat ttggattaga atttcttgac taaattttgt cgtacttttt aataacgaga
      601 atggagagta attttttcta tgtagataat aatttttctg ttagaaatca aaaaaataat
      661 ggaaagggaa cgaattttta ttactggacc ttatttataa tatgatatag ctttactgtt
      721 taacctatcc tgtttaatca taaatctcag tttataattg tttaatgtag actatacaaa
      781 tctaataatt tttgttatag tcttgatcta tatacatcta taattccttt aggacgctgt
      841 ttgcttacca tttaaattgc ccccatatta ttaggttatt aatgattgat tataaaagat
      901 ttcgagatat aagaaattca ccttcaaact cgatgaactt tggttttgaa acttactctc
      961 ttgcggataa agtaatccaa caacagcatg atttacttaa tgatattggt atgtcttctt
     1021 tgactttccc cttcaatcaa agtaaaatag gtgagcctga atcaatggta aaagaagata
     1081 taaatcgcaa aatacgttcc ctatcaaaaa actctgacga tttctataat atagattaat
     1141 tgtacctttt tcatttttta aaagcatttt aagtaacata ttagaatcgt ggatattaat
     1201 tctgtcatat attttacatt ctatgatcga gtctctagtt aattgttaag gctttgacac
     1261 gttgtttgct tgtttcctaa tacgaaatag ctaatttgtg tcaatgagat ttagtttgac
     1321 gattttcttt aaatttaatc tggtatttgt taaatattat catattttaa taatattgaa
     1381 cgtatttttc attaaaatgt cattattagg ttacttttta tcattctttt gatgatagca
     1441 gtctaaattc caattaaagt tttaaggagc ataataatta actctagaat ttgtaaacat
     1501 gataataggt aaaagacctt tcaaaaaacc tttaattttg ttgctactat cctatccgcg
     1561 gagtcggact atccttcagc tatgtcttga ataatataat aggttgtagc ttgttggatc
     1621 gccaggatag ttaaataagt gtttatatct tacttaattt acttaaagtt aactacttgt
     1681 ttcatcttta tttttaaaat acaattctta aactttgtta tttagaaaaa gaccgttatt
     1741 tcgttgccat ctgaaatata tcagtaacaa tttaagatca cagttgaaaa taattcgtgt
     1801 gcaattatgg gaatagaatt tcatgtaaca atattctata gtaaatataa aatgtatttc
     1861 ttgttataat aaattttgta tcttaattta acacttatct agtacttttt tataagatac
     1921 ttagtcggtt gaatgcagtc ctctggttat ttattttatc tgtgaactgc aaaagtaaca
     1981 attttttgat ttaaattttt atctctatta caaagaataa tatcttttaa tggttctgtt
     2041 atttggtacc agccccaatt tttttctata aataatacta aagatacaat aactaatgta
     2101 tcgaatagta ggtatgcctt atagaattag gatttcaaat ggaatagaag aaattgaaat
     2161 ggaatcggat caaatttccg atatagatac catgattgag aaagtagaac aattaaaaca
     2221 attgtttaga gaaaaaccaa gtattgattc ctctccaacg aaagaaaaat cttaccttgg
     2281 aaaatcataa ttaaatttcc aacttcccta aattttaatt ttttatttac aaatattatt
     2341 agtaataaaa aaaattgtca agatattata aaaaaatata tggttttact ttaatggtta
     2401 tctgttattt tatttttgac ctctttattt tttcttgcaa taacatacat ccctgaataa
     2461 gtgtctctgg tcttggcgga catcctggta catagacatc tacaggaata ataccatcta
     2521 ttccaggtaa aacattataa gaatcaatat agagtcctcc agtaatagaa catgctccca
     2581 ttgcaataac atatttggga tctgccattt gatcatatac cattctcaat ctcggagcca
     2641 tctttctagt aacagtacct tgaacaacta ttaaatcaca ctgtcttagg gaaccaaaag
     2701 cttcgataat gccgaatctt tcacaatcat aacgaggact agatgtagca ccaaattcta
     2761 cactacaaca tgcagtttca aggtgtacag gccatagaga ccagattctt ccccaattaa
     2821 ttgcataacc taatggttga tctaaagcgc ctactaaaac atcgctaact tgacttacca
     2881 acaagttaaa gttagctgga ttaataagat ccttaatcat taacatcgac ttcctttatt
     2941 aataaatata ttaaaagata atttaatctt atcaaattag taattttcat taccatattc
     3001 cttttctacc agaaaggtat aaagcatatg ccatggctgc aaacattata gcaagaaagg
     3061 ccatcatagg taaagtagct tcttttggga tagataaaag cgatgaaccc caagcataca
     3121 aaaatattga catcacatca aaaacaacga acatcaatat gtaagcataa tattgcatca
     3181 taaaatgcga acgtccttca cccgttggca cttgtccaca ttccattgga agaaatttca
     3241 ccggattata cctttttcgg ggagaaagta atcgtgccag aattaaggat ggtattccag
     3301 ccataatccc gattccgaag gcatacaaaa ttggagtaaa ttctgatgcc aattcgccag
     3361 ccaaattagt cttgctgcct tatctaggta taaaaatctt tattttttaa taataagatt
     3421 gaaaagttgc cgagcgatta gtttcggatg atttagtgct aactttatca ttttacgagg
     3481 agaaaactct gaactaataa aatctatgaa ttcatctact gacatatgtc ttagtatgga
     3541 gatttcatga tcccattcat catctgttaa gccaatccat cttgattgta cccttaaagc
     3601 agattctatt ttagaactaa gttgtttttt ccattcgttc tcataatgga ttaatgaatt
     3661 ttttgaacaa ctgctaatta gagattcaga gccgatttgc cctgctaatc ttccaaattc
     3721 aatggcatgt ctaatccctt caagcaccag tggattagat tgacctgccg aatctcctac
     3781 taaaattaga ccgtcgaaaa ctgaacactt tcgcgctcct tcattaggaa tatatccata
     3841 gtgatattct attggttgga tatttcctaa ttccataagt ggtttggttt ttttttcaat
     3901 tatttgattc aacaatttaa ggggatctag catagattct ggttttccaa ttcctacacc
     3961 tatgcgtaca cggttaggtg ataaaggaaa taaccatgca tatccagcag gagaatagat
     4021 gctaccaacc atcaaagtcc aggtttcgtg atcgatttga tcacaataac attcgtattc
     4081 agcaccgact ccatatcttt tccatgttga aacatatcct agttttctgc caataaaact
     4141 attaaatcca cttgcatcta taacaaggct tgtccttaac tcgaccttgc ctttaggggt
     4201 attgactatt aatccagaaa tcttattgtt atttttgata cacaatacat cgataacatt
     4261 agagtttatg ctgatttcag cgccagcctt aatggcgaga aaagaaagat actgatataa
     4321 tgatctgaca tctaaaacgc atgatcgata ttcggtccct gtaatagtta tttcgttgtt
     4381 aggagaaaca aaacgataat tctttattgg gttatatatt tcctcaggaa tttctaattt
     4441 tttcatttca ccaatccaag aaacaccact agttcttaca tactgcccga tagatttctc
     4501 acgttcaaat agaataactc tagctcctga cttagctgca gtataagccg cagaaagccc
     4561 agctggtcct ccaccaacta tggcgatatc ataattatac atttccatta cattttctca
     4621 atcgaatttt tattaagttt ttcataaaga taaaggaaac tgagggttat aaaggggaaa
     4681 gttttttatc ggtaaaaaat gtaaaaaact attgagtcaa aagtttcatt taagagaggg
     4741 taatatggct ccagatttca tatttaaaga ttcagataat aacgatttga ggttatcaaa
     4801 ttttttagga aaacgtatcg taatttactt ttatcctaac gatttcactc caggttgtag
     4861 tactcaagca tccgaatttt ctattcactt caataaatat ttggaaaata atataattat
     4921 attaggtatt agtcctaatg acgagacgac tcatcaaaag ttcaaagaaa agatgaggat
     4981 tccatttttg ttaatttccg attctaataa taaaatatct caaaaatatg gtgtatacac
     5041 gcttaaaaaa tttatgggta aagagtatat gggtgtcatg agaactacat ttttaataga
     5101 taaacatggc aaaattgtta aaatctttta taaagtaaaa ccaaagggac atagcgcaga
     5161 agtgttaaat tacttcttga gggataacta gttgaaggta atttcgttat atctatttta
     5221 taaataatag ttgtttaact cttcatccat tttttctata tctaaattaa aaaaaatgca
     5281 aaattcgtta tctccattaa tactattaga aatctctcga aattctactc cttgacggta
     5341 accgttgaca atttctgcta ctaactccaa ggttttcttg gacaaaatat tccatttttt
     5401 aatcgtttca taatctctaa tatgaagtaa ggagataaat ttggtgaatg aacagattat
     5461 cataaatcgc agtttttgaa atactttttt ataaggatta gtatcggcaa tgtgaggaag
     5521 cattgtacac ataagatact gataataatc gaaattcttt aatatgctca ttgattctcc
     5581 aatttgttga aatgtaattt ttaaagagat tatatcatat ttagttttgg tgaaaaaacc
     5641 tggctcagtc tcgatttcaa aataaggaga tgttatccca tccttaacca agaacttttt
     5701 acaataatga gaagaattaa gatagtaggt aaattgatta gatgaatatg acaattttta
     5761 aataatattt aacaaaataa tcactcttaa attttcctag tattatttga tttttttaga
     5821 gtaagattgc accagttttc taatatgttc taatatcttt atatgatgtt cttcgtctac
     5881 aacaacactt tgtaataatt ccttagtatc tttatcttga gccagaccta tacattcaag
     5941 taagatattt ttagaatctt cttcttcctc aatggagtga tttattcctt cctcggttag
     6001 atgaatgctc cctgatgctc cttctatttt cgccatagcc tgagtgatgt attgtatatg
     6061 ttttataccg tccgaaagta gggtatttat ataatttcta attaagttat tttcaatttc
     6121 ttttaggaga gtactataat gtaatatttc atctttttct gcttcatgtt gttgtttcaa
     6181 taattcataa agtctttgct gtttttccat ttttccacga tcttgaacca atattctgat
     6241 taatgttatc acaaataaaa gagtgatggt agcatatttt ctttaccaat gaaaactcct
     6301 agagtgaaaa agattataat cgcagcaaag ttataaaaaa gggtgtcaca taataatgct
     6361 agttgtcaat gtatttgtca tcaacgttat ggtgataagt cattttgtag aaaatgttta
     6421 gataaacata aagcttcacc ttattataat attatgaaaa aatttgactg atttcttaca
     6481 atatgcggga gatgggattt gaacccacga acccctaagg gataagagcc tcagtcttac
     6541 gcctttgacc aggctgggcg acccccgctc agaatcaaga cgtcagagac tctaatttga
     6601 atattacgag tatgaaataa agtgacattc caattataat ggtttttata taattatcag
     6661 ttctttagta gtgtgttaat tccagttcga tgttttactt gtggtggttt aattgcagac
     6721 aaatattttg aatataaaga aagggtcaaa tctggagagg gagatgcaga ggttcttgat
     6781 tcacttggaa taaaaagata ttgttgtaga agaatgttta tttctagtgt agaaacaatt
     6841 tatcaagtaa ttccttatta tgaagttctc cgaagacgaa tgtctgaaat gcaatctgag
     6901 agttaatttt aacatatcat gccgatcatt accgatatca ttagtaggtt gatatttaac
     6961 agtagaggta gtaaaaccat agaggtagac ataatctctg acgataaatt cctcggccga
     7021 gcatcttctc cttcaggtgc aagtgtagga aagcatgaag ctcaaagttt taaagaaaat
     7081 agtccagaga aatcattaga agaactcaat aataatttgt ctaaatttat tggaatagaa
     7141 tctcatgata ctagagcagt atatgatgtt ctaaaaaata ttgataatag tgaaaattat
     7201 ggtaaaatag gtggctctgt tgcttatgca ataagtctag cttccgtgga atctgcttca
     7261 aaagctctca acgtacccct ctttagacta ttgaaaccag taggcgatta taaattccct
     7321 tatcctttag gaaatatttt gggaggaggc tctcatgcag gaccaggaac tccagatatt
     7381 caagaatatc ttatttgtcc aattggagcc tccaatataa tagaggctat agaaattaat
     7441 attagagttc acaaacaatt aaaaacaata ttagaaaaat tagacaataa ttttacctat
     7501 ggtagaggag atgagggggc atgggctcct aatcttaata atgatcaagc tatagaagtg
     7561 gtagaacaag ccattttaga tagtggttat aattcaggca aagaagtagc aataggaatt
     7621 gattttgcaa gttcctcatt ttggaatgaa actacccaaa gttatgatta tcttagacaa
     7681 gggctagttc gatcgacaaa tgaacagatt gatttcgtta acgatcttat agataaatat
     7741 catttaattt atgtagaaga ccctgttcac gaagaagatt ttgagggaat gtctatcatc
     7801 aataaaaatc atccagagtg tctcattacc ggtgatgata tgttggtaac aaataaaaaa
     7861 cgagtaaaag aatctattaa atacaattcc tgttcaggtg ccattttaaa agtcaatcaa
     7921 gccggttctc tttacgaagc attggaattt gtcaatgaat gtaacaaaaa cgatataaaa
     7981 cttataactt cacatagatc tggagaatca atagattctc atatcgcgga aatagcagta
     8041 gctacttctt ctaaattgtt aaaaacgggt gtcattgggg gagaaagagt agctaaatta
     8101 aatcaattag taaggttagc cgagtatgat ttaataaaag ggatgtctga tgttttcaca
     8161 tcttaatatg agcgagaaga cggaaatcat gaacagtgat ttaattacta gtgatacctc
     8221 taatgctgaa accaatgaag ataaagaatt acctgaagct gaaaatttac ctgaagctga
     8281 aaatttacct gaagctgaaa atcaatctac taattcttta aatgaagtcg aaaattcttt
     8341 agtagaaaaa catgatgatg agattaatat agaaaattct gactccgaaa atttagaaaa
     8401 aatgatttta tctactggaa taagagttgg cactccagta aagaccaaat caatgtcacg
     8461 atatatagtc agagcaaatc ctgaaggact atatatttta gacattagta aaacattgta
     8521 taggatagat gttgcagcta aattcatcgg cagaacaaat atcgccaaag ttgctatcac
     8581 ctctgcccgg gagtatggta aaaagccggt agaaaaattt tgtgaagcga ctggtgcaac
     8641 tcctatttta ggaagattta tgcctggaac atttactaat ccttccttac caaaatatat
     8701 ggaacctgaa atagtcatag taacagaccc acaggccgat caacaagctg tcattgaagc
     8761 tactcgagca ggcgttcctg tgattgcaat atcaaatagt gataatgtta tttctaaagt
     8821 tgatttggtt attcctgcta ataacagagg aagaaaagca ttagctaccg tttattggtt
     8881 actagccaga gaagtaatga aaaaacaagg aaaaataaaa tcagataaag aaatgaaagt
     8941 aacaatagat gattttgaaa ctaaacttgt agaagagata gtttgagaga tttaatttgc
     9001 agatctccta attatgcagg ttcctattat cccaaagata aaggtgattt gataaaatta
     9061 cttcattttt gttttaatga aagtatattt gcgccaaaaa aaaaacagac acttaatact
     9121 aaaatattcg gaatcctcgt tcctcatggt gcttatatat attctggagc aatatctgca
     9181 aatgcattct atgaaatcgc atctcaaaat atcgatactt tcatcttgat aggaccagac
     9241 cataagggta ttggaaaaag aatttctgtt acaagtgaag gatattggca aactccctta
     9301 ggagatgtac tactcgatta tacaataatt aatgatttaa taaaatcatg tgattttatt
     9361 gaagaagata aaataaccca ccagattgaa cattctttag aaattcaagt tccattcatt
     9421 cagtattcat gtggtaattc tttcaccatc gtcccccttt tattgcgaga tcaggatatc
     9481 gaaacatcag ttttacttgg aaacttaatt gcaaaattta gtgcaaggaa aaatgcgatc
     9541 attatagcat catcttgttt aactcattat gaatctaatt cgaatgtgca gaagaaggat
     9601 tgccagatga tcaaatgtat tgaaaacttg gatatagaat ccttctatca attgataatg
     9661 aattcgaata cttccgtttg tgggtatggt gcaattgcat cattaatgag agcagtaaaa
     9721 atatcaggat cacaaaaagg ggtattatta agatatgcta ctagcggaga tgtaacgtca
     9781 gacataaaga atacagtagt agggtatgcg tcaatagctt ttgtttaaac ttaatgataa
     9841 tgactagtac cttagcttct gccccagcaa aaattatctt atttggagaa cattttgtag
     9901 tatataatca accaattata ttggcttcta taaataaaag gatgaaaata gagtgtaaaa
     9961 tagaggaaga taagaataat acagttaata tagaaacaaa tcattttgga aaaaaaacat
    10021 ttcctttatc aataatagaa agtaataatc attcgattgt aaacgatttt ttttatccta
    10081 ttatttacat tttaaggaaa atattacgtt attattcaga accttatgga atcactatta
    10141 aaatcgactc agagatacca tatggagtag gcttaggatc ttctgcagct ttaagtgttg
    10201 ctggagtagc tgctatttca ggacttaata atatagttaa ggaaaagaat gaaatcttaa
    10261 acatagcaat tgaaaccgaa aggattatac ataaaaattc ttctggagca gattgtgttg
    10321 tatcaacgta tggtggatta ttgtattatc aaagaaccac aagtataaat atgaggtctc
    10381 tcaacttgaa caatgagct
//
LOCUS       DATAFW010000035         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_11865, whole genome shotgun sequence.
ACCESSION   DATAFW010000035 DATAFW010000000
VERSION     DATAFW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11865"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VHJ57_00840"
     CDS             <1..231
                     /locus_tag="VHJ57_00840"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX2487112.1"
                     /translation="KNRIEMHLISKVKQKVKVNKLNQVFDFEKGETILTENSYKYSLK
                     SIEQLAKKSNLRVERNFVDKNEWFNLALLTPF"
     gene            complement(239..1585)
                     /gene="egtB"
                     /locus_tag="VHJ57_00845"
     CDS             complement(239..1585)
                     /gene="egtB"
                     /locus_tag="VHJ57_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX2487113.1"
                     /translation="MTKVINKVVEDLISEFTEVRKLTDDLFKTLSIEDAVIQSDPFGS
                     PPNWHIAHVTWFFHKILLKYGQKLNLEKINLDYLNSYYQKFEKILPKNDRGRFPRPTV
                     SESLQYRKYLDKKVLLFLEKNDEVSNSLSKEIQYDLQLANQHEMQHQELMIYDFQNYF
                     ERFPDSNDNYCPFKINDNFDKQAQNKKHPKEMVKIPGGIFDIGFYGDGFSYDNELPEH
                     KVYLYPYEIDSTPVSNGEFIKFIEEDGYKRYEYWLADGWDVVQEFKWNSPLYWFKIDD
                     VWMKKDFFGTSAINPYEPVINVSYYEADAYARWAKKRLPTEAEWEKAASWNEDLERKT
                     RYPWGNEIRPTQEHANLLESFLWKPALCGSYEKGKSFYGCYQMIGDVWEWTSSEYSLY
                     PGFKSRFSEYTDKWAINQKVLRGGSFATPRRQIRNSYRNYFKPHERIQFSGFRCAK"
     gene            1726..>2426
                     /locus_tag="VHJ57_00850"
     CDS             1726..>2426
                     /locus_tag="VHJ57_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HEX2487114.1"
                     /translation="MIRRLVKNSFKSIDEGLYNIKDIDYSHEPLNLKKNILDIKTKNK
                     IPLITEIKFSSPSKGVIMNRDNVDLLRISREMTEGGAAGLSVLTQKYLFNGSLTNFIM
                     IRQDTSIPMLMKDIIVSEIQLDCAKKIGADYILLIMSIFDKNLAEGDLDRFVKYANKK
                     GLNILFEVHHEHEFNEILKFNLGKADLIGINNRNLENLKIDLQITEKLLKKFNKAKNV
                     IISESGITNSNQIKQL"
BASE COUNT          867 a          351 c          347 g          861 t
ORIGIN      
        1 aaaaatagaa ttgaaatgca tttaatttca aaagtgaaac aaaaagttaa agttaataaa
       61 ctaaatcaag tttttgattt cgaaaaagga gaaaccattc ttacagaaaa ttcttacaaa
      121 tactcattaa aatcaataga acaattagct aaaaaaagta atttaagagt agaaagaaat
      181 tttgtagata aaaatgaatg gtttaatcta gcattattaa caccatttta aaagaattct
      241 actttgcaca tctaaatcct gaaaattgta ttcgttcatg aggcttgaag tagtttctat
      301 aactatttct aatctgtctt ctaggtgtag caaacgatcc accacgtaat actttttgat
      361 ttattgccca tttatcggta tattcagaga atctagattt aaaaccagga tataatgaat
      421 attcagaact tgtccactcc caaacatcac cgatcatttg ataacagcca taaaaactct
      481 ttcctttttc ataggaacca cataaagcag gtttccacag aaaagattct aacaagttcg
      541 catgttcctg agtggggcga atttcattgc cccatggata ccttgttttt ctttctaaat
      601 cttcattcca actagcggct ttttcccatt ctgcttctgt aggtaatctt ttctttgccc
      661 atcttgcata ggcatcagct tcataatagc taacgttaat tactggttca tatggattaa
      721 tagctgatgt tccaaagaaa tcttttttca tccaaacatc atctatttta aaccaataga
      781 gtggagaatt ccatttaaat tcttgtacaa catcccatcc atctgccaac cagtattcat
      841 acctcttgta accatcttct tctataaatt ttataaactc accgttagat acaggagttg
      901 agtctatttc ataaggataa agatatacct tatgttctgg taattcatta tcatatgaaa
      961 atccatctcc ataaaatcct atatcaaaaa ttcccccggg aatttttacc atttctttag
     1021 gatgtttttt attttgggct tgtttatcaa aattatcatt tattttaaag ggacaataat
     1081 tatcattaga atcaggaaat ctttcaaaat aattttgaaa atcatatatc atcagttctt
     1141 gatgttgcat ttcatgttga tttgcaagct gtaaatcata ctggatttct ttacttaaag
     1201 aattagatac ttcatcattt ttttcaagaa ataacaagac ttttttatct aaatattttc
     1261 tgtactgaag actctctgaa acagtaggtc ttggaaatct acctctgtca tttttaggca
     1321 aaattttttc aaacttttga taataggaat ttaaataatc taggttaatt ttttctaagt
     1381 ttagtttttg gccatacttt aatagtattt tgtgaaaaaa ccatgttacg tgtgctatgt
     1441 gccaatttgg aggactgcca aaggggtcag attgaataac ggcatcttct atacttagtg
     1501 ttttaaacag atcatcggtt agctttctga cttcggtaaa ttccgagatg agatcttcta
     1561 ctactttatt tattacctta gtcacaggtt ttataatact attggatgca ataaatttta
     1621 cttttcttta caaatgatta tacataatag atataatagc aagttactga tggttattta
     1681 ggtggagttc aaacgagtaa atttttttct tctaatagcg aaaatatgat caggaggtta
     1741 gttaaaaatt cttttaagag tatagatgaa ggattataca atataaaaga tattgattat
     1801 tctcatgaac ctcttaattt aaagaaaaat attttagata taaaaacaaa aaacaaaata
     1861 cctcttatca ctgaaataaa gttttcttca ccttcaaaag gggtaattat gaatagagat
     1921 aatgtagact tgttaagaat atcaagagaa atgacagaag gaggtgcagc tggtctttca
     1981 gtcttgaccc agaagtattt gtttaatgga tctttaacta actttattat gattagacag
     2041 gatacgtcca ttccaatgtt aatgaaagat attattgtaa gtgaaataca attagattgc
     2101 gccaaaaaaa ttggagcaga ttatatttta ctaattatga gtatttttga taaaaatctt
     2161 gctgaaggtg atttagatag atttgttaaa tatgctaaca aaaagggcct aaatatttta
     2221 tttgaagttc accatgaaca tgaatttaac gaaattctaa aatttaattt gggtaaagca
     2281 gatttaatag gaattaataa tcgtaattta gagaatttaa agattgattt acaaattaca
     2341 gaaaaattat taaagaaatt taataaggca aaaaacgtga ttattagtga aagtggaata
     2401 actaattcta atcaaataaa gcaact
//
LOCUS       DATAFW010000036         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4593685, whole genome shotgun sequence.
ACCESSION   DATAFW010000036 DATAFW010000000
VERSION     DATAFW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593685"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VHJ57_00855"
     CDS             complement(<1..193)
                     /locus_tag="VHJ57_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487115.1"
                     /translation="MEHSQTKADYELLLDRLHNKLGTTAKKEVSRLELPVPQIIWVGQ
                     RTIFRNFMDFPKALRRDPEK"
     gene            complement(494..1090)
                     /locus_tag="VHJ57_00860"
     CDS             complement(494..1090)
                     /locus_tag="VHJ57_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487116.1"
                     /translation="MVVVTDSFMGIFTPIDIPSRIIQFIEKKIEFPFIKEDEIVGIFY
                     LFGKDNKVTSDLEILSVRNIAKRTMEQQARNIKIFDEFKENKLDSKFIRVEYNKRILQ
                     ISIEEQISPSSSAHLRYRIAGDPTIQSNCFEQHIAYYKQDYFYELFKQPFKKIEVPSH
                     LISKLEKRMLMLCYNVSDSSKLPYTNTVHPFFQWLNDQ"
     gene            complement(1219..>1638)
                     /locus_tag="VHJ57_00865"
     CDS             complement(1219..>1638)
                     /locus_tag="VHJ57_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEX2487117.1"
                     /translation="NEEVKAAYAARGEEQVPIGVSGTMVAVDWDSCVADGACIEACPV
                     QVFQWYRTEKDVPAKDAINETYEGTGSSVKEERKDYTDKADPIREHDCIWCMACVSVC
                     PPQAIKVDQAALEFHEKAQGTYNESISTGGAPPPHAH"
BASE COUNT          541 a          244 c          229 g          624 t
ORIGIN      
        1 atttttctgg atcgcgacgt aatgcttttg gaaaatccat aaagtttcta aatattgttc
       61 tctgaccaac ccaaattatt tgtggaacag gtaattccaa tctagagact tcttttttag
      121 ctgttgttcc taatttatta tgaagtctat caagtaataa ttcgtaatct gctttagttt
      181 gagaatgttc cataattaat ttacattttt cttattaatg tacgttgtgg tagctatata
      241 aaaagaggat tttaagacga ttgaaataat aagaataaga ataataaatg attaatacaa
      301 tatgtacata taattagatg aattttattg cttttaaata aaatagttaa ataactagca
      361 tcagaacatt gtagaaaaat ctctttttat taattatcaa gatgtaattt aattgttaaa
      421 tctaaaaaat ttttttgttt aagtgaatca tagatataaa agaaaaaagt tctctagcag
      481 aaataaattt tatttattgg tcatttaacc attgaaaaaa tggatgaaca gtattggtat
      541 aaggtagttt tgaggaatca gacacattat aacataacat aagcattctc ttttccagtt
      601 tactgattag atgagatgga acttctattt ttttaaaggg ttgtttaaat agttcataaa
      661 aataatcttg tttataatat gcgatatgct gttcaaaaca gttggattga attgttggat
      721 ctcctgctat tctatacctt agatgggcag aggaagatgg ggatatttgt tcttctatgc
      781 ttatttgtaa tattcttttg ttatattcta ctcttataaa cttagaatca agtttatttt
      841 ctttaaattc atcaaagatc ttaatgtttc tagcttgttg ttccatagtc ctttttgcta
      901 tatttctaac agatagtatt tctaaatcac tagttacttt attatctttt ccaaataaat
      961 aaaatattcc aactatctca tcttccttaa taaatggaaa ttcaattttt ttttcaataa
     1021 attgtattat tctagaaggt atatctattg gagtaaatat tcccataaag gaatcagtta
     1081 ctacaaccat ttattatcgt ctaagataat actttataca aaatagagtt attaacaact
     1141 aattaataat aatctgatat attacctaaa agacctaaat gacatagagt aattcattat
     1201 ataaataaaa aaaaagattt agtgagcatg tggtggtgga gcgcctcctg tcgaaattga
     1261 ctcattatat gttccttgtg ctttttcatg aaattccaat gcagcttgat ctactttgat
     1321 tgcttgtggt ggacaaactg aaacgcacgc catacaccat atacaatcat gttctctgat
     1381 aggatctgct ttatctgtat aatcttttct ctcttccttt actgaagagc cagttccttc
     1441 atatgtttca ttaatagcat ctttggcagg aacatccttt tctgttctat accattgaaa
     1501 tacttgaact ggacaggctt caatacatgc accgtcagct acacaagaat cccaatcaac
     1561 tgctaccatg gtgccactta cacctattgg aacttgttct tcgcctcttg cagcataggc
     1621 tgcttttact tcttcatt
//
LOCUS       DATAFW010000037         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2832308, whole genome shotgun sequence.
ACCESSION   DATAFW010000037 DATAFW010000000
VERSION     DATAFW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832308"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VHJ57_00870"
     CDS             complement(<1..349)
                     /locus_tag="VHJ57_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX2487118.1"
                     /translation="MAASVIVESLKKMEYRGYDSVGIATLEKDRILVRKDVGKVLDVD
                     SSLQLNNMPGQIGIGHTRWATHGGITKLNAHPHSDCTNNVVVVHNGIIENYQQLKETL
                     IKQGHIFQSQTDSE"
     gene            552..1508
                     /locus_tag="VHJ57_00875"
     CDS             552..1508
                     /locus_tag="VHJ57_00875"
                     /inference="COORDINATES: protein motif:HMM:NF016980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487119.1"
                     /translation="MRKKTIISDGNGNPLSKKILIFENINDMKILSNNIAWKIVELLS
                     SSAMYPAQIAKELKLYDQTVYYYIRKLARIGAIEQVGTCLIRGGTARLYSTSSPSFGL
                     EIEGNEEKLESSNYIKDEKRKNIPFILKEFYENNSFSGLIVVGAPDPHGPYKSSSRDG
                     HYAVHLSFYLGTLAESYTSGFIVKLDVDAKAEKDIDNRNLILIGGPGTNIVTSEFNRY
                     LQIKFNEDNYWSGLTDQSGRIFNMDNHGLIAKINNPYNKDKKILILAGVRSIGTKASV
                     IALTDYGNQISNNSLSDDQLALVVQGFDMNADGKIDHVDIVS"
     gene            complement(1512..2018)
                     /locus_tag="VHJ57_00880"
     CDS             complement(1512..2018)
                     /locus_tag="VHJ57_00880"
                     /inference="COORDINATES: protein motif:HMM:NF003139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX2487120.1"
                     /translation="MGDPKKSRKTFRRPRQIWTTDQLSSELYLIGSYGLRSKSELWKA
                     QTKVADIRNQARELLALTVEARQAQETKLLSHLQRLGLIKDSATLDDILNLKLEDILE
                     RRLQTIIMKKDNAKSPHQSRQLVVHGHVSIGERTINIPGYIVKKDEEAKIALRAQSMN
                     PSQNMPGS"
     gene            complement(2018..2500)
                     /locus_tag="VHJ57_00885"
     CDS             complement(2018..2500)
                     /locus_tag="VHJ57_00885"
                     /inference="COORDINATES: protein motif:HMM:NF003140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX2487121.1"
                     /translation="MSTADYKHIVRIMGKDVEGDKKITVALSKVKGVGYNFAQVLLQK
                     LNINPYIRVGLLTDKELTDIESVLKDPNKIGIPYFYFNRRKDMDTGSNIHLLTSDLDF
                     IVSNDIDREKSIMSWRGYRHMFGLRVRGQRTRTTGRRASAVGVRKTAQAPLKTEKSGE
                     "
     gene            2847..>3185
                     /locus_tag="VHJ57_00890"
     CDS             2847..>3185
                     /locus_tag="VHJ57_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487122.1"
                     /translation="MNFKDIEKMVLDSYNSNPTYNEIFPGVPHNYKEIKTLKDLLEIN
                     YRHANVDTQIRGNLITLLKKGINPYRGIIGYEDDVIPAINRGILAGHDLLLVGQIGQA
                     KTKLSEAISQN"
BASE COUNT         1096 a          508 c          473 g         1108 t
ORIGIN      
        1 cttcactatc agtttgactc tgaaaaatat gtccttgttt tataagagtc tcctttagtt
       61 gttgataatt ttcaattatt ccattatgaa ctacaactac attattggta caatctgaat
      121 gaggatgtgc atttaattta gttatacctc catgagtagc ccatcgtgtg tgtcctattc
      181 ctatttgtcc aggcatattg tttaattgta aagagctatc aacatccaga acttttccta
      241 catctttacg aactaaaatt ctgtcttttt ctagggtagc gatcccaaca ctatcatacc
      301 ccctatattc cattttcttc aaactttcaa ctataaccga cgcagccata cacttaccaa
      361 tatagcctat tatagaacac ataaataata tcaaatagaa ttagtttaaa tagtatattg
      421 gtattcattt tataccccta tacaaataat tataatcaca aatctatact ttaaatcaat
      481 ttatagaaaa accactatga taattggtga ccggtataaa acattttata ggaaatatac
      541 aataacctat tatgagaaaa aagactataa tctcggacgg gaacggaaat cctctaagta
      601 aaaaaatttt gatatttgaa aatataaatg atatgaaaat tttatcaaat aatattgctt
      661 ggaaaatagt agagctatta tcatccagtg ctatgtaccc cgcacaaata gcaaaggaat
      721 taaagcttta cgatcaaaca gtgtattatt atattagaaa attggctaga ataggagcaa
      781 tagaacaagt aggaacatgt ttgatcagag gaggtacagc tagactttat tcgacttcta
      841 gtccctcatt tggattagaa attgaaggaa acgaagagaa acttgaatct tcaaattaca
      901 taaaagatga aaagcgaaaa aatattcctt ttatattaaa agagttttat gaaaataact
      961 ctttttctgg tttaatagtt gtgggagcac cagatcctca tggtccttac aaatcttcct
     1021 cacgggatgg gcattatgct gttcatttga gtttttattt gggaacttta gctgagtcat
     1081 atacctctgg attcatagtt aaattggatg tagatgcgaa agcagagaag gatattgata
     1141 atagaaattt aatattgatt ggtggacctg ggacaaatat tgttacttca gaatttaaca
     1201 gatatcttca gattaaattt aatgaagata attattggtc aggattaacc gaccaatcag
     1261 gaagaatatt taatatggac aatcatgggc taattgctaa aataaataat ccttataaca
     1321 aggataaaaa aatcctcatt ctagcaggtg ttagaagtat aggaacaaaa gcatcagtta
     1381 tcgccttaac cgattatgga aatcaaatat cgaataattc tttaagtgat gatcaattgg
     1441 cattagttgt tcaaggtttc gatatgaatg cagatggaaa aatagatcat gttgatatcg
     1501 tttcttaatt attaactgcc tggcatattt tgagatggat tcatggattg agctcttaaa
     1561 gcaattttcg cttcttcatc tttcttaaca atatacccag gaatgttaat agtcctttca
     1621 ccgattgata catgaccatg cactactaat tgcctagatt gatgcggaga tttggcatta
     1681 tcctttttca taatgatggt ttgcaatctt cgttctagaa tatcttccaa ttttaaatta
     1741 agaatatcat ctagtgttgc agaatctttt attaatccaa gtctttgtaa atggcttaat
     1801 agtttagttt cttgtgcctg tcttgcctca acagttaatg ctaataattc acgtgcttga
     1861 tttcttatat ctgcgacctt agtttgtgcc ttccataatt cacttttgct tctaagacca
     1921 taagatccta taagatacaa ctctgaactt aactgatcag tagtccatat ctgacggggt
     1981 ctacgaaaag tttttctaga tttcttagga tctcccatta ttcaccactc ttttctgttt
     2041 ttagtggagc ctgagctgtt ttacgtacgc cgactgcgct agctcttcta ccagtagtac
     2101 gtgtgcgttg tccccttaca cgcaatccaa acatatgtct atatcccctc caactcataa
     2161 tacttttctc tctatctata tcatttgaaa caataaaatc caaatctgat gtaagtaaat
     2221 gaatattgga acctgtatcc atatcttttc tcctgttgaa atagaaatat ggaataccta
     2281 ttttattagg atctttgaga acgctctcga tatcagttaa ttctttatca gttaaaagac
     2341 cgactctgat atatggatta atatttaatt tttgaagtaa tacttgagcg aaattataac
     2401 ctacaccttt aactttactt aaagctacag ttattttctt atctccctca acatctttac
     2461 ccataattct aacaatgtgt ttatagtctg ctgttgacaa ctcttaaaat tagagtataa
     2521 acctaaataa aaatcatact aggagttaat ttaacgtaat taattattta taatctcatg
     2581 tatcctcgtt ttaaataaat taagttaaat gatatattta taaaatttgc atggtattta
     2641 tttttgtcaa aataatatac cttttaaaac tatttaaatc aaaaagatta taaaaacaga
     2701 ataaaactta attgttactt ttcagagtaa gaagtaaaat gaccaaatag aatccttagt
     2761 ttttattagc atgaatatta ttaatgattg ctattagcta attctttaac aatgtttaaa
     2821 ttttgtttca gttagcaaga aatctattga attttaaaga tattgagaaa atggtattag
     2881 actcttataa ttctaatcca acatataatg aaatatttcc tggtgttccc cataattata
     2941 aagaaattaa gacattaaaa gatcttcttg agataaacta taggcatgcc aatgtcgata
     3001 cacagattag agggaattta ataacgcttc ttaaaaaagg tattaatcct taccggggaa
     3061 tcattggata tgaggatgac gtaataccag caataaatcg aggtattctt gctggtcatg
     3121 atctgttatt agtaggacaa ataggtcaag cgaaaacgaa attatctgaa gctatatctc
     3181 aaaat
//
LOCUS       DATAFW010000038         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_894481, whole genome shotgun sequence.
ACCESSION   DATAFW010000038 DATAFW010000000
VERSION     DATAFW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894481"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VHJ57_00895"
     CDS             <1..1068
                     /locus_tag="VHJ57_00895"
                     /inference="COORDINATES: protein motif:HMM:NF045300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487123.1"
                     /translation="KELVRLNVVPCYKVEQGQWKSSADRSQYHTEYMMKILTLEQKNQ
                     IRILKAFLKGIGIYGAELSIAGLSGYVTEVLILKYGNFKNVIKNIAMVEKKGKVIAID
                     NIPNNITEKFQSPIIIIDPVDSNRNLGSAISPESLSRFIFGARVFLKKPSINFFSINS
                     KKDLINFDKDLQKLSELLQYILVLEFKFKSRSSDIIWGQLKKLTLSLARHLNDKEFEA
                     IKSECYIYNNNSAKVLFLLKFNKLPHFVKKTGPSIFMEKEMFKFVEKNKEKSALIWIG
                     DDMRLLCLKKIEITDAIKFLERSIRLDKRKLGIPKGLLNDLNKGFKIYKINKNTLKNL
                     HNQRIINDFLFKDLSLFHATD"
     gene            1133..>1635
                     /locus_tag="VHJ57_00900"
     CDS             1133..>1635
                     /locus_tag="VHJ57_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487124.1"
                     /translation="MMDLYTMGVEHAVLEGPTKLCELNILGKGHSGIVLKVNTYSKKT
                     LALKIRRLDSRRKDTLTEVYNQKLANQEEIGPKIIDNTEDLVLMEFISGKGIGDWLQD
                     PTYFHLLSSKNIISIVNEILEQCYRLDVLNLDHGELTRIDNHVIISETNQISIIDFES
                     ASTKRKP"
BASE COUNT          665 a          200 c          266 g          504 t
ORIGIN      
        1 aaagaacttg taaggttaaa tgtagtacca tgttacaaag tagaacaagg tcaatggaaa
       61 agctctgctg atagatcaca ataccataca gaatatatga tgaaaatact tacccttgaa
      121 caaaaaaatc aaatcagaat cttaaaagcc tttttaaaag gtattggaat ctatggagct
      181 gaactatcaa ttgctggatt aagtggttat gttactgagg ttttaatact aaaatatgga
      241 aatttcaaaa atgttattaa aaatattgca atggtggaaa agaaaggtaa agtaattgct
      301 atagataata ttcccaataa tattactgaa aaatttcaaa gtccaataat aataatcgat
      361 cctgtagatt caaatcgtaa cttgggatcg gcaatatctc ctgaaagtct ctcgagattt
      421 atttttggcg cgagagtttt tttaaaaaaa ccctcaatta atttttttag tattaatagc
      481 aagaaagatt tgataaattt tgataaggat ttacagaagt tatcggaact attacaatat
      541 attttagttt tagaatttaa atttaaaagt agaagttctg atataatttg gggccagtta
      601 aaaaaattaa ccttatcact tgcccgacat ttaaatgata aagagtttga agccataaag
      661 agtgaatgct atatttataa taataattcg gcaaaggtat tatttttatt aaaatttaac
      721 aagcttccac attttgttaa aaaaactggt ccaagtatat ttatggaaaa ggagatgttt
      781 aaatttgttg aaaagaataa agaaaaatca gctcttatat ggataggaga cgatatgaga
      841 cttttgtgtc taaaaaaaat agagatcact gatgcaatca aatttttaga acgatcaatt
      901 agattagaca aacgtaaatt gggaatccct aaaggtttgc tcaatgattt aaacaaagga
      961 tttaaaatat ataaaataaa caaaaacaca ctaaaaaatc tacataacca gagaattatt
     1021 aacgattttc tatttaaaga tttgagttta tttcatgcta cagattaact caccaataat
     1081 ctcaaagtta atttcatatc caaaattaga cgtaagggaa tatcataaga gaatgatgga
     1141 tttatacaca atgggagtag aacatgctgt attagaaggt cctactaagt tatgtgagtt
     1201 aaatattttg ggaaaagggc attcaggtat agtattaaaa gtaaatacat attctaagaa
     1261 aactctagca ttaaaaataa gaagactaga ctctcgtaga aaagacactt tgactgaggt
     1321 ttacaatcaa aaattagcta accaagaaga gataggtcca aaaattatcg ataatacaga
     1381 agatttggta ttaatggaat ttataagtgg aaaaggaatt ggtgattggc ttcaagaccc
     1441 aacctacttc catcttctca gcagcaaaaa tatcataagt atagttaatg aaatattaga
     1501 acagtgttat aggctagatg ttctcaattt agatcatggc gaattaactc ggatcgataa
     1561 tcatgtaata atttctgaga ctaatcaaat atcaataata gattttgaaa gcgcatctac
     1621 taaaagaaag ccaag
//
LOCUS       DATAFW010000039         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4947300, whole genome shotgun sequence.
ACCESSION   DATAFW010000039 DATAFW010000000
VERSION     DATAFW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4947300"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VHJ57_00905"
     CDS             complement(<1..569)
                     /locus_tag="VHJ57_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX2487125.1"
                     /translation="MTVTEGKLIIHNFESSVLKSNPLKDPYNREIIVYVPHDYSPSRS
                     KGFPAAVGLSGFSGNGRTFFNLDPFSENIQQRMNRLISEKKCGSMILVIPNCFTKFGG
                     NQYINSTATGRYEDYLVDEIIPFIQNNYNISSFGVWGKSSGGYGSLVLGMRYPELFKA
                     IACHSGDAAFEYCYLPDFPNAYQSFKQTGG"
     gene            complement(667..1593)
                     /locus_tag="VHJ57_00910"
     CDS             complement(667..1593)
                     /locus_tag="VHJ57_00910"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2487126.1"
                     /translation="MNLYKSGISLEVIAFQTDLDVQEVQNIVSREEQKNSDEKIYTAP
                     SLSEFYLDAIVDIDELIKNAQARTWSALQAKEFNISTEDSRQILENLLESKTRLVIIH
                     VDLVGFTKLCMSLPANRLADIIRAFTQEVSILISSYGGYILKFVGDAVLGFFLVKSSE
                     KNDDKLPCINAVNCALSMISVIRHGINPILSQYDYPEIHVRIGVDVGENVVVQYGWET
                     NILKHGTEKILTKKPIIDILGYTISIAVKMTSLAESDQIVIGQMVYDSVDKTLKERFK
                     EFPIHTEVWNYISSTTGGIYRLYGSIRDHTYY"
     gene            complement(1740..2030)
                     /locus_tag="VHJ57_00915"
     CDS             complement(1740..2030)
                     /locus_tag="VHJ57_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487127.1"
                     /translation="MTAINANKKIYVIVHGIRQNYDKVKSMLIEKQLNEEKIAMASLE
                     KAGEIGEYVAMTWPPMAAKEVIVSEIVGTNSEGKGMGAWGSIDQKELFRFPF"
     gene            complement(2107..2376)
                     /locus_tag="VHJ57_00920"
     CDS             complement(2107..2376)
                     /locus_tag="VHJ57_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487128.1"
                     /translation="MIEQRFHGLFLPLVVSGIIISTFGIFLNFLGQGIKHGEGFEIIG
                     IRFLIAGIATIALGYFAQYYTKIRNSLLIFKYFLENINKKTKSSR"
     gene            2458..3390
                     /locus_tag="VHJ57_00925"
     CDS             2458..3390
                     /locus_tag="VHJ57_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX2487129.1"
                     /translation="MNIAVLGSNEFLHEFGKRGTTSDIVLYDRKLNDRIFTFIAPISF
                     PDKIQTLPQVLNMTDFVVLNITQLDKYLAEQIIAINSLGIRNGFLLHSYDIDEQKLDQ
                     MIKGTTIENFKKIESLDILKNEINNLHSNSREGDLLMPIDHSFDVKGVGTVLLGGIKQ
                     GKIKLYDKLRILPLNKEVLIKSIQMHDDPVDESSSPSRVGLAVKGVLPRDIERGDIIC
                     TGDNLKTADTEVLAIDFSKNKYYKGEITDTQTYLLSVGLQIKPLKIAINQSKILLSLE
                     KPIAFYEKQKFLILKPDSKTTRIIAEGSFPPINQ"
BASE COUNT         1300 a          578 c          482 g         1343 t
ORIGIN      
        1 cctccggttt gcttaaaact ttgataagcg tttggaaaat caggtaaata acaatattca
       61 aatgctgcat ctccagaatg gcaagcaatc gccttgaata attcagggta acgcattcct
      121 aaaacaagtg aaccatatcc acccgaagat tttccccata caccaaaaga cgaaatattg
      181 taattatttt ggatgaatgg gattatttca tcaacaagat agtcttcata tcttcctgta
      241 gcggtagaat ttatatactg atttcctcca aattttgtaa aacaatttgg aataactaaa
      301 atcatagaac cacatttttt ttcagaaatt aatctattca ttctttgctg aatgttttca
      361 ctaaaaggat ctaaattgaa aaaagttcta ccattaccac taaatcctga aagtccaaca
      421 gctgctggga atcctttaga tcgactaggt gaataatcat gaggaacata tactatgatt
      481 tctctattat acggatcttt caaaggatta gattttaaca ctgaactttc aaaattatga
      541 ataattaatt ttccctctgt aacagtcatt gttgatacta taatctgtct taatatataa
      601 tgtgcaaatt aaattaaatt aaattaataa aactgaattg gaaataaagt aaaactagat
      661 taaattttaa tagtaggtat gatctcttat acttccatat aatctatata ttccacctgt
      721 agtactagat atataattcc atacttccgt atgtatagga aattctttaa atctttcttt
      781 tagagtcttg tcaacagaat catataccat ttgtcctatc acaatctgat cagattcagc
      841 aagtgatgtc attttaactg caatacttat tgtatatcct aaaatatcaa taataggttt
      901 ttttgttaat attttttcag tcccatgttt taagatattt gtttcccatc catattgaac
      961 tacgacattt tctccgacat caactcctat cctaacatga atttcaggat aatcgtattg
     1021 actcaaaata ggattaattc catgtctaat tacagagatc attgagagcg cacaattaac
     1081 tgcattaata caagggagtt tatcgtcatt tttttcagaa gatttaacta aaaaaaatcc
     1141 taaaaccgcg tccccaacaa attttaatat gtacccacca tatgaggata ttaaaataga
     1201 tacctcttga gtgaaagctc gtattatgtc tgctaatcgg ttggcgggaa ggctcataca
     1261 aagcttggta aatcccacta aatctacatg tataataact agtcttgttt tagattcaag
     1321 caaattttct aaaatttgtc tagaatcttc tgttgaaata ttaaactcct ttgcttgtaa
     1381 tgctgaccat gttcttgctt gagcattctt aattaattca tcaatatcta ctatagcatc
     1441 aagataaaat tctgataaag aaggtgcagt ataaattttt tcatctgagt ttttttgttc
     1501 ttccctactt actatatttt ggacctcttg gacatctaaa tctgtttgaa atgcaattac
     1561 ttcaagtgaa attcctgatt tgtaaaggtt caaaacagtc tgtttttgtt caattgatat
     1621 aacatatcac cttttataat ttataataga aggatgataa aataacatat tattgtattg
     1681 acactgtaaa ttaattactt aattaatata tttgatatct taattttatc atatcatcat
     1741 cagaatggaa acctaaataa ttctttctgg tcaattgatc cccacgctcc cattcctttt
     1801 ccttcactat tagtccctac aatctcactt actattactt ctttagctgc cataggaggc
     1861 catgtcatag ctacatattc tccaatttct ccagcttttt ctaaggatgc catcgcaatt
     1921 ttctcttcat ttaattgttt ttctattaac atacttttca ctttgtcata attttgtcta
     1981 attccatgaa caatcacata gattttttta ttagcattga ttgctgtcat aatttctcta
     2041 cttaaataat atataaaagc ttacctttcc gacaaaggct attcatagtt taagaattca
     2101 aagattttat ctagaggact tagttttttt atttatattt tctaaaaaat atttaaaaat
     2161 aagtaaagaa tttcttattt ttgtatagta ttgtgcaaaa tatcccaggg ctattgtcgc
     2221 aatcccagca attaggaatc taattcctat tatttcaaaa ccttccccat gtttaatacc
     2281 ttgacctaaa aaatttaaaa atattccaaa agttgatata attataccag aaactactaa
     2341 aggtaaaaac aacccatgaa acctttgttc aatcatttca attatgcatt gaaataaact
     2401 caacaaattt ataaacaatt ttattgatta tttattattt ttttgctatt ggaaagtttg
     2461 aatatagctg ttcttggaag taatgaattc cttcatgagt ttggaaaacg aggtactaca
     2521 tctgatatag tgctttatga cagaaaatta aatgatcgaa tatttacctt tatagctcca
     2581 atttcttttc cagataaaat ccagacgtta cctcaagtct tgaatatgac cgattttgta
     2641 gttttaaata ttacacaatt ggataaatat cttgcagaac aaataatagc tataaatagc
     2701 ttaggtatta gaaatggttt tttgctccat tcttatgata tagacgaaca aaaacttgat
     2761 cagatgatta aaggtactac aattgaaaat tttaaaaaaa tagaaagtct ggatatttta
     2821 aaaaatgaga taaataatct tcactcaaat tcaagagaag gagatctttt aatgccaatt
     2881 gatcatagtt ttgacgtcaa aggtgtaggg acagttttac ttgggggaat taaacaaggg
     2941 aaaattaaat tatatgataa actgaggatt ttgcctttaa acaaagaggt tttgattaaa
     3001 tctattcaaa tgcatgatga cccagtcgac gagtcttcaa gtccatcacg ggtagggtta
     3061 gcagttaaag gagttttacc tagagacata gaaagagggg atattatttg cacaggagat
     3121 aacttaaaga cagcagatac agaagtatta gcaatagatt tttcaaaaaa taaatattat
     3181 aaaggagaga ttacagacac acaaacatat ttactttcag taggtttaca aattaagcca
     3241 ctaaaaattg caattaatca atctaaaata ttattatctt tagaaaaacc gatcgccttt
     3301 tatgaaaaac aaaaattttt aatattaaaa ccagatagta aaactacaag aataatagct
     3361 gaaggatcat tccctcccat aaatcaataa gtttgatatg taattaaacc tggtctacta
     3421 tattcagtag ttggttgttt aatactctgt aatgtgacta aatatagata tatagtaatt
     3481 tataatatta ccattttttt ataatgaaat aagccctcat tctattgaag actttaattt
     3541 ttttctagtt tctcgattag attaatttta aattttttgt attgttattc attttaaaag
     3601 aatctttaag gttattattt taaatggacc tatctgaata ataattgtgt cattttctat
     3661 ctttttgatt tcttgaatct cattttccat aaagtctatt aac
//
LOCUS       DATAFW010000040         5219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1071589, whole genome shotgun sequence.
ACCESSION   DATAFW010000040 DATAFW010000000
VERSION     DATAFW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071589"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VHJ57_00930"
     CDS             complement(<1..422)
                     /locus_tag="VHJ57_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487130.1"
                     /translation="MLSSEIQSPKIESIKSFDIADIAIMSQLAYSFKDEFQKYFKVDS
                     FTSPDPFNNEDGNIYLVIVDRENIDRIISMIIIRNDSYNSIPLEKIMNDRLKIIQISK
                     EKALSLKNEMMPKDTNNFYQFRKEDGRVIGYIMFAFQI"
     gene            complement(490..1497)
                     /locus_tag="VHJ57_00935"
     CDS             complement(490..1497)
                     /locus_tag="VHJ57_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX2487131.1"
                     /translation="MKTSIQVNQLDLLDRNILNEIQWCFPFKERPFLEIANHHNIPEN
                     EVMKRIHNLKQMGLIRQINAIFDTRRLGYKSALIAFAVKPEKLDYIANEINKYSGVSH
                     NYERNHEFNIWFTLAVPPEKDMKEILDKMSSLDGVIKYRLLPTLKMYKIGVKLDMVNN
                     DSSNPKPTETVKELNPEKSKLTDLDKEFIKELQKDIEIIPEPFKKSAEKLGLTQQELF
                     KKAKEFEENGIMRRFAAILRHRDAGFTANGMIVWKVPEEVINKVGYKLAEFPQVSHCY
                     RRPTYPDWKFNIFSMIHARSIEAAEKIAKEISCIIKIYDYKILFSSREFKKERVKYFE
                     E"
     gene            1775..2434
                     /locus_tag="VHJ57_00940"
     CDS             1775..2434
                     /locus_tag="VHJ57_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HEX2487132.1"
                     /translation="MKNKKDIDDNLNDVIEFDNDNYDKDTNELESNNTDTLSKDEKTK
                     NAKKVNSTTINSQSDLSIKNNHNVDIQDSDSKFFAIRITGGQENVVANILQNKINSKR
                     ISLRSILILENFKGYVIVEAPDSNIAYDALYGVRHVRGQIRGDLPFSDIEKYLVKKPV
                     VTELSIDDTVEVIAGPFKSMKAKIIRVDYEKQEATVVLLDSPYQIPVTVDANYLKKAS
                     G"
     gene            2515..2997
                     /locus_tag="VHJ57_00945"
     CDS             2515..2997
                     /locus_tag="VHJ57_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX2487133.1"
                     /translation="MSDKKTVSALVTGGEASAGPPLGPALGPLGINVLQVVNTINDKT
                     KDFSGMKVPVKVEVDSETKKFTVEVGIPPTTALIAKEAGITKGSGTSGKDYVGNISME
                     NIVKIARMKIDVSYALDLKNASKEIIGSCISSGVRIENKPAKEIYADIEKGQFDSLFN
                     "
     gene            3131..4792
                     /gene="thsB"
                     /locus_tag="VHJ57_00950"
     CDS             3131..4792
                     /gene="thsB"
                     /locus_tag="VHJ57_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX2487134.1"
                     /translation="MSIQQGSAGGVPVLILKEGSTQTKGRDAQKNNITAAKLIAEIVR
                     SSLGPRGMDKMLVDTLGDVTITNDGATILKEIDVQHPAAKMMVEISKATDNEVGDGTT
                     SVVVLAGALIEKAEDLITNDVHPTIIVDGYRKSAQKAIEILNKIAQKIDAKDKSDLLK
                     IAKTAMQTKLVSKEAPELAELVVNAIFQVSDKSDSKYKVDIDDVKVEKKTGGSLRDTT
                     LIQGIVLDKEVVHGGMPKRVENAKIALLNCALEIEKTEFDAKINISSPDQMKMFLEEE
                     NRILKDMVDKIKNTGANVVICQKGIDDIAQHYLAKSGILTVRRVKESDMTRLARATGA
                     RVVNNIDDLVPGDLGNTDLVEERKVETDKWVFVEGCKHPKSVTILIRGGSQRVIDEAE
                     RSIHDALMVTKDVLEKPFIVAGGGSPEAYVAGKLREWTSSLSGREQLAAEKFADALEV
                     IPITLAENAGMDPIDTTTDLRAKQSKSSKWMGIDVKTAKIADISKLEIFEPLSVKEQI
                     IKSATEVACMLLRIDDVIASSKSAGGGMPPGGGMPPGGMGGMGEM"
     gene            complement(4798..5016)
                     /locus_tag="VHJ57_00955"
     CDS             complement(4798..5016)
                     /locus_tag="VHJ57_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487135.1"
                     /translation="MRCIICEKERIETSEYCIYHTRARKYLQASHKKWNEAYSTNLTW
                     DQYLDKLSKLESVGIWIKQVIDIELKKS"
BASE COUNT         1858 a          735 c          841 g         1785 t
ORIGIN      
        1 catatctgaa atgcgaacat aatatatcca ataaccctac cgtcttcttt tctaaactga
       61 taaaaattgt ttgtatcttt tggcatcatt tcatttttca atgacaatgc tttctcttta
      121 gagatttgta ttatcttcag tctatcattc attatctttt ctagtggtat tgaattatag
      181 gaatcattac gtatgataat cattgaaatt attctatcta tattttctct atcgactatt
      241 actaaataaa tattgccatc ttcattatta aagggatcag gagaagtaaa ggaatcaact
      301 ttaaaatatt tttgaaactc atccttgaag gaatatgcaa gttgactcat aattgctata
      361 tctgctatat caaaagactt gatggattct atttttgggg attggatttc agaagataac
      421 acaattatct tttaattatt tgatatatat tttattttat taaacgatta ttcaaaatta
      481 ttaaattgat tactcctcaa aatattttac tctttctttc ttaaattctc ttgaactgaa
      541 tagaatctta tagtcataga ttttaattat acatgaaatt tctttggcaa ttttttcagc
      601 agcttctata cttctcgcat gtatcatact aaatatatta aatttccaat ctggataagt
      661 tggtcttcta taacaatgac ttacttgggg aaattctgcc aacttgtatc ctactttatt
      721 tattacttct tctggtacct tccagactat cataccgttt gcggtaaatc ccgcatctct
      781 atgtctcaag atagcagcaa aacgtctcat tatcccgttt tcttcaaact cttttgcttt
      841 tttaaataat tcttgctgtg ttagtccaag tttctctgca cttttcttga aaggctctgg
      901 aattatttct atatcttttt gtagttcttt tataaactct ttatctaggt cagtcaattt
      961 gcttttttca ggattaagtt ctttaaccgt ctcagtaggt ttaggatttg aagaatcatt
     1021 atttaccatg tcaagtttaa cgcctatctt gtacattttc aatgttggca aaagcctata
     1081 ttttattact ccatcaaggg aagacatttt atctaaaatt tctttcatgt ctttttccgg
     1141 aggtactgct aaagtgaacc atatgttgaa ttcgtgattc ctttcataat tatgactaac
     1201 accagaatat ttgtttattt catttgcaat ataatctaat ttttcaggtt ttaccgcaaa
     1261 agctattagt gcactcttgt atcctaatct tctagtatca aagattgcat taatttgtct
     1321 aatcaatccc atctgtttga ggttatgtat tcttttcata acctcatttt ccggtatgtt
     1381 atgatgatta gctatctcta aaaacggtct ttctttaaaa ggaaaacacc actgaatttc
     1441 atttaaaata tttctatcga gtaaatccaa ttgatttact tgtatagatg ttttcaattt
     1501 tggttgaata atgtataata tctattaaat acttatccat ttattctaac cttattattt
     1561 aaatataata atagtataat tataaaaaaa tttgtagttt aattataata cctttattac
     1621 atctcaaatc taatgtattt ataattaatt gttaatagca aaactatcta ttaacaataa
     1681 aagtaatttt gtttcttttc acacatatac taacaattaa aaatataaaa taaatttata
     1741 atatactgta atcgtaaaat attgtattat attaatgaaa aacaaaaaag atatagatga
     1801 taatctaaat gatgttatag aatttgataa tgataattat gataaagata ctaatgaact
     1861 agaatctaat aatacagata ctttatctaa agatgagaaa actaaaaatg caaaaaaagt
     1921 taattctact actataaata gtcagtcgga tctatcaatt aaaaataacc ataacgtaga
     1981 tattcaagat tctgattcaa aattttttgc aattagaata acaggtggtc aagaaaatgt
     2041 cgttgcaaat attttacaaa ataaaattaa ttcaaaaaga ataagtttac gttctattct
     2101 tatcttggag aatttcaagg gttatgttat tgttgaagca cctgattcaa atattgcata
     2161 tgatgcatta tacggagtta gacatgttcg tggacaaata cgaggagatc ttccttttag
     2221 tgatattgaa aagtatcttg taaagaaacc agtagtaaca gagttaagca tagatgatac
     2281 agtagaagtt atagctggac cttttaaatc tatgaaagca aagattatac gtgttgatta
     2341 tgaaaaacaa gaagctactg tagtattact tgattccccg tatcaaattc ctgttactgt
     2401 agatgcaaat tacttaaaaa aagctagtgg atagtctata caaaagtgta acctaaacat
     2461 aattttaatt acatacgatg acgatttata tttcggaaaa atattatttt tgacatgagt
     2521 gataaaaaaa ctgtctccgc tttggtaact ggaggagaag ctagtgcagg accacctctt
     2581 ggaccagcat tgggaccact tggaattaat gtcttgcaag ttgtaaatac tattaacgat
     2641 aaaaccaaag atttctcagg aatgaaggtt cctgttaaag tagaggttga ttctgagaca
     2701 aaaaaattta ctgtagaagt gggtatacct ccaactaccg cattaatagc caaagaagct
     2761 ggtatcacta agggatctgg aacttcagga aaagattatg tcggaaacat tagcatggaa
     2821 aatatagtta aaattgcaag aatgaagata gatgtatctt acgcacttga cttgaaaaat
     2881 gcctctaaag aaataattgg atcctgtatc agtagtggtg taaggattga aaataaacct
     2941 gctaaagaaa tttatgcgga tatagagaag ggtcaatttg attccttatt taattaatat
     3001 ttgtattatt tttactaaat atttactgca attgatgaat aacctctata ataaaaattg
     3061 tattgcagaa aattaaaata tccccagttc tgtcacattg ttataaataa ttttaggtga
     3121 gaatgataga atgtcaattc aacaaggttc agctggtggt gtaccagttt taatattaaa
     3181 agaaggatcc actcaaacaa agggaaggga tgcacaaaag aataacataa cagctgctaa
     3241 actaatagca gaaattgtcc gttcaagtct tggtccccgt ggtatggata aaatgttagt
     3301 agacacatta ggtgatgtta ccatcacaaa tgatggcgct actattctta aggaaataga
     3361 tgtacaacat cctgctgcta aaatgatggt agaaatcagc aaggcaacgg ataatgaagt
     3421 tggtgatgga actacttctg tagtagtatt agcaggtgca ttgattgaaa aagctgagga
     3481 tcttataaca aatgatgttc atccaactat tatagtagat ggatatagaa aaagtgctca
     3541 aaaagctatt gaaattctta ataaaatagc acaaaaaata gatgcaaagg ataagagtga
     3601 tttacttaaa atagccaaga cagcaatgca gactaaactt gtctcaaagg aagctccaga
     3661 attagctgaa cttgtagtaa atgcaatttt ccaagtatca gacaagtctg attcaaaata
     3721 caaagttgat attgatgatg ttaaggtaga aaagaaaaca ggtggatcat taagagatac
     3781 tacattaatt caaggaatag tactagacaa ggaagtagtt catggtggca tgcctaaaag
     3841 agttgaaaat gccaagattg cattattaaa ttgtgctttg gaaattgaaa agactgaatt
     3901 tgatgcaaaa ataaacatta gctctccaga tcagatgaaa atgtttcttg aagaagaaaa
     3961 cagaatatta aaagatatgg ttgataagat taaaaataca ggtgctaatg tggtaatttg
     4021 tcaaaaaggg atcgatgata tagctcaaca ctatttagca aaatctggta ttctcacagt
     4081 tcgtagagtt aaagaaagtg atatgaccag attagcaaga gctactgggg ctcgtgtagt
     4141 gaacaatata gatgatctag tgcctggtga tttaggaaat actgacctag ttgaagaacg
     4201 aaaagttgaa actgataaat gggtatttgt tgaaggttgt aaacatccaa aatccgtaac
     4261 cattttaata agaggaggat ctcagagagt catagatgaa gcagaacgct ctattcatga
     4321 tgcactgatg gtaacaaaag atgttttaga aaaaccattc attgttgctg ggggtggatc
     4381 tccagaagct tatgttgctg gaaaacttag agaatggaca agttcattat ctggacgaga
     4441 acaattggct gctgaaaaat ttgcagatgc tcttgaggta atacctatca cattagcaga
     4501 aaatgcagga atggatccta tagatacaac aactgatctt agagcaaaac agagcaaaag
     4561 ttccaaatgg atgggcatcg atgttaaaac tgccaagatt gctgatattt caaaattaga
     4621 gatatttgag ccattaagtg taaaagaaca gataattaaa tctgcaactg aagtagcttg
     4681 catgctttta cgaattgatg atgtgattgc atccagtaaa tccgcaggtg gaggaatgcc
     4741 acctggtgga ggaatgccac caggtggcat gggtggaatg ggagaaatgt aaaatctcta
     4801 actttttttt aattctatat caatcacttg cttaatccaa ataccaacac tttctaattt
     4861 tgacaattta tctagatatt gatcccatgt taaatttgta gaataagctt cattccattt
     4921 tttatgactt gcttgaagat attttctggc tcgagtatga taaatacaat attcagaagt
     4981 ttcaattctt tccttttcac atataataca tctcattata tttaattata tatatataat
     5041 atattaaaaa agaattaaat taatctcaat aatattttaa aaaaggtatt tatatttatt
     5101 ttaaatgcta tcttttattg aactatagca aatcaaaact cattattgta atatctgtaa
     5161 tttatctggt tttctttttt gcattatcaa ctggattaat aaatgctctt cttgaaggt
//
LOCUS       DATAFW010000041         5489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_367455, whole genome shotgun sequence.
ACCESSION   DATAFW010000041 DATAFW010000000
VERSION     DATAFW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367455"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1568)
                     /locus_tag="VHJ57_00960"
     CDS             complement(258..1568)
                     /locus_tag="VHJ57_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX2487136.1"
                     /translation="MNTSRKFPSFDGIENFEQKTARYRINSEKTVPVTGPPGTGKSTV
                     ISHCCFDLLNDDYFPILVTAPTNVMIDSILSKIDSLLQREKIQLPSGFVIRYGNVANL
                     NRTYNHLIKYAFDNIVEDYCSSGYSNCADNNNFYNKITIAKDFFQSARIILSTDYSAK
                     DLGNIVKVGAVIVDEAGLVGLDRMGMLFSSLRDNNGKIIVIGDDKQLPPVSHDYVTES
                     LFGSVLEHFSTTLLKREYRFNKDILDLINPYYEYKLEADSSVMNISTADIANKDYDGI
                     NNNLYRVLKHDKNVVFIDTGGEYKEEKHFTNKGEVAIIKEIVDGCISMGIKDIIVTTP
                     YKQQERLCNFYLQRGIRIGTIDEFQGQEAEVTIISMVRSNNKTNYQEAIGFVNIPRSC
                     VAFSRSKRKTIVVGDRNTLMKNNFLSHSIDTITQKDGFLLWRKF"
     gene            complement(1574..3253)
                     /locus_tag="VHJ57_00965"
     CDS             complement(1574..3253)
                     /locus_tag="VHJ57_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487137.1"
                     /translation="MINNHSKWDFNPNNILNTNNIKNVDSDYQVRNFNSSNKNVPKLN
                     SFQKDIDNDYTLTSKDVIRKINPLVLPRISAIANIGLCERAAYNISFFGMESDNNFTA
                     EGVIGNAIHRITLRSIMEITESLKNGSNNTINSSTNIATKKSKAKEIFLRNAERDIKI
                     NWKRFMLSNIENPLPSILDDLEVRAERLVHQLFAKEEEEENKQIIFRPEFTIRNIKIP
                     LEGRLDLVKIKLSNTQREKGSKNYNGIEHLNSYVTAEDLVNLEKESVEIVQIKTGNYR
                     PRTTIWNLQADAEALLLMQTLNLKEPPKYTWQFADKDGNRKKFDFTKVYKVIDKYIQI
                     WKSEVSPQITGFCPKCPLREGCLNWAFVGNNALTEDERIKRNIEFRLSKRIREEVSLD
                     DRWKAYVVLQSAEKRQREGYAITNLYIDISSIDAEKQKITLLGDEYFGQFLDFSVGEH
                     VTISDGTPNLGSNPNAVIRAIDIDRKSITLEFYRDDLYYLLYDNRDKSPLTIDRFNFS
                     SGLISMKFLDSFFRLSSYADDLVLKKRSVRQEEQNIIDMNRKGLLNMNRLL"
     gene            complement(3250..4293)
                     /locus_tag="VHJ57_00970"
     CDS             complement(3250..4293)
                     /locus_tag="VHJ57_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487138.1"
                     /translation="MRTFNDQEDEIEFSSIFPFRISHISPSSASYIKHINKNNIYNYD
                     SSNFDSSFKIFTERLLKEPFHFEPNNYQSGYAIPSILNQVVVVPYYEIIDGNIIQNVT
                     GKEKLGTVTKQTTTIKAQKLKYIIDASTFTNSKIILVCKVKTVDQARDFLADYLKIIG
                     FVEINKNWDFVYNESDSIMQQLLLSLYDYWPTNESQTTSIKIGGKTISANYSGRGKYD
                     LRLEEVNDEIKNRIINGDHIENITLKPPIGIAGIKFRKSVPKISINDQGVISCRANIN
                     YGNFVIIKWFIEETINIIKQLYKVKQPKASYIKIEDFITQEIAHVTDSKLPIYDNSLK
                     INNNEKILRGRND"
     gene            complement(4297..>5489)
                     /locus_tag="VHJ57_00975"
     CDS             complement(4297..>5489)
                     /locus_tag="VHJ57_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2487139.1"
                     /translation="VKEAFNRGVIRGQSQSNFFEFFDFIGNPFDSSFLITNQRQIVKS
                     TRVIINKLAERIGACHKNERHLILVGPECSGRSTILKIIENFLNKGFNDNFSLYVNAQ
                     DSWSGFTTEEDGNDDHFDRIDNFQQWVKEVDFQNSRIILVDDADSFISHAVQYCNAIK
                     LDGFKVPTFIYCITNVTYSYVINNERLSKIFGDVFWLGIREEDDIKKIILESPKNTLK
                     SNTKHLKFFDDYSLNQIVNYSFGLPGLTSFFILSCLKTAYQASSNKIERKLIKRVADI
                     EGFTLAKKIIDRNLNLDGTKYKILVEILRQYYIDGERAERKKIISQFSNMARSTLAYH
                     FKDLVNENVIQQDRIGLRVYYTIQKPIRCALELIDLGLAEIETRNESDINEIVNIDQT
                     IGDV"
BASE COUNT         1755 a          910 c          698 g         2126 t
ORIGIN      
        1 tagacaaaga caaattctat attcctaaaa atttactaga gcgttttgat ggcgatacag
       61 tctatttaag aataacagag gaagaagcaa aacaatacaa aaaagactag cttcttacaa
      121 acttaatttt taagaattat ctttatgtaa tacattttat ttaataaatt ttttctttat
      181 tttattataa ttaatctctc cataccaatc acaacaagta acttcaataa ataaacagca
      241 ccgctacatt atgattatta aaattttctc cataaaagaa atccatcttt ttgagttatt
      301 gtgtctatcg aatgagataa aaagttattt ttcataagag tatttctatc tcctactact
      361 attgtttttc tcttactcct tgagaatgca acacatgaac gtggaatatt aacaaatcct
      421 atagcttctt gataattggt cttattgtta gatcgaacca ttgatattat tgtaacttct
      481 gcttcttgac cttgaaattc atcaattgtt ccaattctta ttcccctttg tagataaaaa
      541 ttacatagtc tttcttgttg tttatatggc gttgtaacaa taatatcctt aattcccata
      601 ctgatacatc catcaacgat ctctttgata attgcgactt ctcctttatt tgtaaaatgc
      661 ttttcttctt tatactctcc accagtatca ataaatacaa catttttatc atgtttaagg
      721 actctatata agttattatt gattccatca taatctttat tagcgatatc tgcagtagat
      781 atattcataa cagaagaatc agcttcaagt ttgtactcat aataaggatt gatcaaatct
      841 aaaatatctt tgttaaatct gtattctctt ttaagtaatg tagtactaaa atgttctaaa
      901 acagacccaa ataggctttc agttacataa tcatgggata ctggtggcag ttgtttatca
      961 tctccaatga caataatttt accattatta tctcttagcg aggaaaataa cattcccatc
     1021 ctatcaagac caactaatcc tgcctcatct actataactg ctccaacttt aactatatta
     1081 ccaagatctt tagcactata atctgtactt aatattattc tagcgctttg aaaaaaatct
     1141 ttggctatgg tgatcttgtt atagaaatta ttgttatcag cacaattaga atagcctgaa
     1201 ctacaataat cctctacaat attgtcaaag gcatatttaa taagatgatt atatgttcta
     1261 tttagattag caacgttacc atatctaata acaaatccac taggcagttg aattttttcc
     1321 cgctgtagaa gagagtctat ttttgataaa atgctatcaa tcatcacatt cgttggagca
     1381 gtaactaaaa ttggaaagta atcatcattt aacagatcaa aacaacaatg tgagataaca
     1441 gttgattttc ccgtaccagg gggtccggtg acaggaactg ttttttctga atttattcta
     1501 tatcttgctg tcttttgctc aaaattttca attccatcaa aggatggaaa ctttcgacta
     1561 gtattcattt tgtttataac aatctattca tattaagtaa tccctttctg ttcatatcta
     1621 taatattctg ctcttcttgc ctaacacttc ttttctttaa tactaaatca tcagcatagg
     1681 aagatagtct aaagaaacta tcaagaaatt tcattgatat taatccacta ctaaaattaa
     1741 atctatcaat agtaagaggt gatttatctc tgttatcgta caaaagataa tacaaatcat
     1801 ctctgtaaaa ttctagtgtg attgattttc tatcgatatc aattgctctt ataactgcat
     1861 taggattact tcctaaattt ggagtaccat cagaaatagt cacatgttca cctacactaa
     1921 aatctaaaaa ttgaccaaaa tattcatcac ctagcaaagt tattttttgt ttctccgcat
     1981 caattgagga gatatcaata tacaaatttg taatggcata tccttccctt tgtcttttct
     2041 ctgccgattg taaaactaca tatgccttcc atcgatcatc aagtgaaacc tcttccctta
     2101 ttcttttcga aagacgaaat tctatgtttc ttttaattct ttcatcttct gttaatgcat
     2161 tattaccaac aaatgcccaa tttaagcatc cttctcgcaa tgggcattta ggacaaaaac
     2221 cagtaatttg tggagagacc tcagacttcc aaatttgtat atatttgtct attactttgt
     2281 atacttttgt aaaatcaaat ttttttctat taccatcttt atcagcaaat tgccatgtat
     2341 atttaggagg ttctttgaga tttaatgttt gcatgagtaa aagagcctca gcatctgcct
     2401 gaagattcca aatagtagtt cttggtcgat aattgcctgt ttttatttgt actatttcta
     2461 ctgattcttt ttctaaatta actaaatctt ctgcagttac atatgaattt aaatgttcaa
     2521 ttccattata gttcttactt cccttttccc tttgtgtatt tgaaagctta attttaacta
     2581 aatcgagtct cccttcgagt ggaattttga tattacgaat tgtaaattcg ggtctaaaaa
     2641 tgatttgctt gttctcttcc tcttcctctt ttgcaaataa ctgatgcact aatctttcag
     2701 ctctaacttc caaatcatct aggatagaag gtaaaggatt ttctatatta gaaagcataa
     2761 aacgtttcca attgattttg atatctcttt ccgcattcct aagaaaaatt tctttagctt
     2821 tagatttttt tgtggcaata tttgtagaag aattgatggt attattacta ccatttttta
     2881 atgattctgt aatctccatt atagatctta aagtgattct atgaattgca ttacctatta
     2941 ctccttcagc agtaaaatta ttatctgatt ccatgccaaa gaaagagata ttataagctg
     3001 cccgctcaca gaggcctata ttagctatgg cacttatcct aggaagtact aatggattta
     3061 tttttcttat aacatctttg gaagttaaag tataatcatt gtcaatatct ttttgaaagc
     3121 tatttagctt gggaacattt ttatttgaag aattaaaatt acgaacctga taatctgaat
     3181 caacattctt gatattattt gtattaagta tattattagg attgaaatcc cattttgaat
     3241 gattattgat caatcattcc ttcctctgag aatcttttca ttattattaa tttttaatga
     3301 attatcataa attgggagtt tcgaatctgt gacatgagct atttcttggg tgataaagtc
     3361 ttcaattttg atatatgatg cttttggttg tttgacttta tatagttgtt ttattatatt
     3421 gatagtttct tcaataaacc actttattat gacaaaattt ccatagttaa tatttgctct
     3481 gcaactgata actccttgat catttatact aatctttggt acagactttc tgaatttaat
     3541 tcctgctatg cctattggtg gtttaagtgt aatattttct atgtggtcac cattaataat
     3601 acggtttttt atttcatcat taacctcttc taaccttaaa tcgtactttc ctcttcctga
     3661 ataattagct gagattgttt tacctcctat ttttatagaa gttgtctgac tttcgtttgt
     3721 aggccaataa tcataaagtg agagaagtaa ttgctgcata atagaatctg actcattata
     3781 tacaaaatcc caattcttat taatttccac aaatcctata atttttaaat agtctgcaag
     3841 gaagtcacga gcttggtcta cagttttaac tttacaaact aaaattattt tactatttgt
     3901 aaatgtactt gcatcaataa tatactttaa cttttgagct ttaatggttg tagtctgttt
     3961 agtgactgtg cctaattttt ctttaccagt tacattctga attatgtttc catcaataat
     4021 ttcataataa ggaactacca caacttgatt taaaatagaa ggaatagcat aacctgattg
     4081 atagttatta ggttcaaagt gaaaaggttc ttttaaaagc cgttctgtaa agattttaaa
     4141 tgaagaatca aaattagagc tatcgtaatt atagatgtta tttttattta tatgtttaat
     4201 ataactagca ctagaaggtg agatatgact aattctaaat ggaaaaatag acgaaaattc
     4261 tatttcatct tcttgatcat taaaagtccg caattcttat acatctccaa tggtttgatc
     4321 aatatttaca atttcattaa tatcagattc atttcttgtt tctatttctg ctaaacctaa
     4381 atctattagt tctaatgcac atctaatagg tttttgtata gtataatata ccctaagacc
     4441 tatgcggtct tgctgtatta cattttcatt taccaaatcc ttgaaatgat aagcaagagt
     4501 agatcttgcc atatttgaga attgagatat gatttttttt cgctcagctc tttctccatc
     4561 aatataatac tgtcttagaa tttcaactag aatcttatat ttagttccat ctaaatttaa
     4621 gtttcgatct attatttttt tggctaaagt aaatccttca atatctgcta cccttttaat
     4681 taattttctt tctattttgt tgctactggc ttgataggca gtctttaagc atgatagaat
     4741 aaagaaactt gttaaccctg gtaatccaaa cgagtaatta actatttggt tcaaggaata
     4801 atcatcaaag aattttaaat gtttagtatt tgattttaat gtattttttg gagattctaa
     4861 aataattttt tttatatcat cttcttccct tataccaagc cagaaaacat ctccaaatat
     4921 tttgcttaat ctttcattat ttatcacata agaatatgtt acatttgtaa tacaatatat
     4981 aaaagtagga actttaaatc catctaattt tattgcatta cagtactgta ccgcatgcga
     5041 aatgaaggaa tcagcatcat caacaagaat tatcctacta ttttgaaaat caacttcttt
     5101 tacccactgt tgaaaattat caattctatc aaaatgatcg tcatttccat cttcctcggt
     5161 tgtaaatcca gaccagctat cttgtgcatt aacatataga gagaaattat cattaaaccc
     5221 tttatttagg aaattttcaa ttatttttag gatggttgat ctacctgagc attccggacc
     5281 tactaaaata agatgtcttt catttttatg acatgcgcct attctttccg ctaatttatt
     5341 tatgattact ctggtacttt taacgatctg tctttgattt gttattaaaa aacttgaatc
     5401 aaatgggtta ccaatgaagt caaaaaattc gaaaaagttt gactgtgatt gtcctcttat
     5461 tacaccacga ttaaatgctt ctttgaccg
//
LOCUS       DATAFW010000042         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_368264, whole genome shotgun sequence.
ACCESSION   DATAFW010000042 DATAFW010000000
VERSION     DATAFW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368264"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VHJ57_00980"
     CDS             <1..594
                     /locus_tag="VHJ57_00980"
                     /inference="COORDINATES: protein motif:HMM:NF028207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX2487140.1"
                     /translation="PPNVHIHKTEKGGISANIHQDSQISESMVKEMCRIYGIHNGRIT
                     IRDPNLSTDDLIDALAGNRVYPHCLIVLNKIDLIDANTLNDMIPFLGEDIEPVSANSN
                     LNIEVLKESIFNKLEFMRIYMRPKGKETDYTEPLIVRKGTKVKDICTKLHRSMLRDFR
                     FALVSGKSAKFDGQKVGLDHELFDQDVLTIIKKINAI"
     gene            complement(602..1144)
                     /locus_tag="VHJ57_00985"
     CDS             complement(602..1144)
                     /locus_tag="VHJ57_00985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX2487141.1"
                     /translation="MLENKDKKYSIIKDIAIIVIGVVLIWFAVKVVFNVDNPFYVVSS
                     GSMIPVLNVGDILIVKDGNTFNNLNVGDIIVFNRPQGGDRVIVHRIIEISDRFTEKVI
                     VTKGDANDGIIPGTDFPIREKDYIGTVAYTVPKVGLVLKYLNPPVNYIIIAGIIGILI
                     YTNIKKKNEGKGKIDGKDPV"
     gene            complement(1291..1686)
                     /locus_tag="VHJ57_00990"
     CDS             complement(1291..1686)
                     /locus_tag="VHJ57_00990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX2487142.1"
                     /translation="MLNVHIDGASRGNPGPSGIGIIIFRNNFLILNYGEFIGFHTNLL
                     SEYLALKRALQFALLIDREIKIHTDSMTLVEQRQSKERIRRKELKILFREINNLEKKF
                     KKISYNFIPREKNTVADLYAKKAIDYYIN"
     gene            2097..2669
                     /locus_tag="VHJ57_00995"
     CDS             2097..2669
                     /locus_tag="VHJ57_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487143.1"
                     /translation="MPKRRFKPTKPAKKIGKKQLLLPPVMIGVATLAGIIIMQLSPPP
                     QVLGICLKAHNIETFNIYPVVQIFENDKQKFLPEGVGKEIKDGKECIHIIHTDEVGDR
                     IHIEYIRPIRLTIDDFMQLYSVDNKTISVINNETGSLNKEILTLDNYDIQYSYYSENN
                     EFTKITNSTLMPPFTKDMVVKIELKTKMWK"
     gene            2732..3757
                     /locus_tag="VHJ57_01000"
     CDS             2732..3757
                     /locus_tag="VHJ57_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX2487144.1"
                     /translation="MGEKKAVVIKGDGTGPELVNCMIDVLKACNSTIELIPVEAGSEF
                     WKKNKTNSYITDEVWKLLSESDACFKGPTTTVPDPDAPRSVAVSIRQKFALYANIRPI
                     KTYKTSQLNLNFVCVREATEGLYAGIEFKTSDDSAVAIRKITRKGCERVVKKAFQLST
                     ELDFKKIFVITKRNILKETDGIFVSAVEKIKKDFDTIATEEYYIDNVTQQLVKNPQRF
                     NNSILLSTNLFMDIISECASGHVGSIGNVYSGNYGDKYAMFEPAHGSAPKYAGQNKVN
                     PVATICSAAWMEEYLGEKDISKAIFKATEDVINENKYVTYDLGGNASLSKMAQQISQR
                     ASKILKK"
     gene            complement(3790..4317)
                     /locus_tag="VHJ57_01005"
     CDS             complement(3790..4317)
                     /locus_tag="VHJ57_01005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HEX2487145.1"
                     /translation="MYIPNVDSFLLIDSIKDYSGQSALEIGTGTGIVSKFLLKNFKNV
                     ISTDIDYNSLLYCKKQSNSEITLICCDATTALSSKFDLIVSNPPYLPNDQFDDKTIHG
                     GISGIEKTIHFIQSAIPNVKKYGHIILIVSSLADISKLDRLIFEKKMNIKIINKKKIF
                     YETLYVYELSSISLS"
     gene            complement(4340..5053)
                     /locus_tag="VHJ57_01010"
     CDS             complement(4340..5053)
                     /locus_tag="VHJ57_01010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HEX2487146.1"
                     /translation="MNWRKYYGQNFLIDSSVVRKIIEISEIKAFEDVCEMGTGKGILV
                     PYLCKNARFVKSFEVDYKLYEKIKLLQSKFKNLKIINEDLLNYIKPLNFDVFISNIPY
                     SKSKEIFLWLATKKFTRAIIMVQDEFSQKIQSNPGDKNYRALSAIIQYCFDIQPLFKV
                     DRKSFYPQPTVDSEVIKLISKNNILSKDTISKVFTIFSFKNKKISKLNNKLKINFNEI
                     DLRVKEMEPEKIIALSKLM"
     gene            complement(5050..5655)
                     /locus_tag="VHJ57_01015"
     CDS             complement(5050..5655)
                     /locus_tag="VHJ57_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HEX2487147.1"
                     /translation="MSLPDHYQDHKGNAFGSFHVTRKYEEYAHIIEFTIRGKSSTVKK
                     REGIIIQAIGEERLTLLELLGDPSQNFEIGERAYIGKEGRNKIISVLGRLNYNDLSVS
                     AKNEIPIAIEKIVTIKEKRFVDYFNNAQPITPRVHGLELIPGIGKIYMKSIITEREKK
                     KFESFNEIQERVGIKDLQKLITKRIVEEISGETRMNLFVRK"
     gene            complement(5679..6002)
                     /locus_tag="VHJ57_01020"
     CDS             complement(5679..6002)
                     /locus_tag="VHJ57_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4"
                     /protein_id="HEX2487148.1"
                     /translation="METLKKEVISLSEVKDILEKVGVEEMDQIQRWTYDYVSKFAKSP
                     SHVSRTIINQLVSSCDLKEDEAVEIANAMPNSIEELRAFTFGWKKLILTDTLNKILDI
                     INKEH"
BASE COUNT         2081 a          937 c          871 g         2256 t
ORIGIN      
        1 ccccctaatg ttcatataca caaaactgaa aaaggaggga tttcagccaa tattcaccaa
       61 gattcacaaa tttcagaatc aatggttaaa gaaatgtgta gaatctatgg aatccataat
      121 ggtagaatta caatcagaga tcctaatttg tctaccgatg atctaattga tgcgttagct
      181 ggaaatagag tatatcctca ttgtctgata gttttaaata aaatagatct aatagatgct
      241 aatacattaa atgatatgat acccttccta ggtgaagaca ttgaacctgt ttctgcaaat
      301 tcgaacctga atattgaagt actaaaggaa tcgattttta ataaattaga attcatgagg
      361 atatacatga gaccgaaagg caaagaaacg gattatacag agcctttaat tgtaagaaaa
      421 ggaacaaagg ttaaagatat ttgtactaaa ttgcacagaa gtatgctccg tgattttaga
      481 tttgcattag tttctggaaa aagtgcaaaa tttgacggac aaaaagtagg tctggatcat
      541 gaattgttcg atcaagatgt tttaacaatt atcaaaaaaa taaatgctat atgaattccc
      601 cttaaactgg gtcctttcca tctattttcc ctttcccttc attcttcttt tttatattcg
      661 tataaattag aattccgatt attccggcta ttattatata atttactggg ggattgagat
      721 atttcaaaac taatccaacc ttgggaactg tataggctac agtcccaatg taatcttttt
      781 cccgaatggg aaaatctgtt ccgggtatta taccgtcgtt tgcatcaccc tttgttacta
      841 tgactttctc cgtaaatcga tcactgatct ctataattct gtgaacaata accctgtcac
      901 ctccttgggg tctattaaaa acaataatat cgcctacatt taagttatta aaggtatttc
      961 catcttttac tattaatatg tctccgacgt tcaaaacagg aatcatactt ccactagaga
     1021 ctacataaaa tggattatct acattaaata ctactttgac agcaaaccat atcaaaacta
     1081 ctccaataac tattatagcg atatctttta taatactata cttcttgtcc ttattttcaa
     1141 gcaattctaa ttaaatttca agttatttgt tacataaact tttagattaa atgttatata
     1201 tggcagctca gacaaatgcc ttcattacat gtgttattca atattaactc tgattcttca
     1261 tagctgccta attaataaat ctgattctat ttaattaata taatagtcta ttgccttttt
     1321 tgcatataga tctgcgactg tatttttttc tcttggtata aagttgtaag aaattttttt
     1381 aaattttttt tctaaattat taatttctct gaaaagaatt ttcaattctt ttcttctaat
     1441 tctttctttt gattgcctct gttcaactaa tgtcatacta tctgtgtgaa tcttaatttc
     1501 tctatcaatt agtaatgcaa attgtagggc tctttttagg gctagatatt ctgataatag
     1561 atttgtatga aatcctataa attcaccata atttaatatt aagaaattat ttcggaatat
     1621 tattatccct ataccagaag gtccaggatt tcctctggat gcaccatcaa tatggacatt
     1681 aagcaagaga gaaataataa ttcaacaact aatatattat ttttcatatg gattttaatt
     1741 gtttttacta gttttcactc aaaatccgag aatgttagtt tgtttccatt caaggaattt
     1801 attgtcgtct ataattttta aatcatgttt cattatggtt ttataaatag aaataagtga
     1861 tatcaagatg aaaattataa tatcatatta ttggattaag aaggtgatat gcattgaatg
     1921 ttttaatgtg tttacattcc aacattgaat atctattgag caacaaaaaa atatacatac
     1981 aatacgtaag caattaagaa attgtgctat atttataatg ttaattatta gcagaattta
     2041 accaaaatag agggtagaaa gactctaata ttgaattttt acgatcctta tttacaatgc
     2101 ccaaacgccg gttcaagccc acgaaacctg ccaaaaaaat agggaaaaaa caattattat
     2161 taccccctgt tatgatagga gtagcaactc tagcaggtat tatcataatg caattatcgc
     2221 ctcctccaca agtattgggt atttgcttaa aagctcataa tatagaaact tttaatattt
     2281 atccagtagt gcaaattttt gaaaatgata agcaaaaatt ccttcctgaa ggcgtaggaa
     2341 aggaaataaa agatggaaaa gaatgcattc atatcattca tacagatgaa gttggggaca
     2401 gaatacatat tgaatatatt agacctatta ggcttacaat tgacgatttt atgcagctct
     2461 actcagtgga taataaaacg atatcggtaa ttaataacga aacgggatca ttaaataaag
     2521 aaatactcac gttagataat tacgatatcc aatattctta ttactccgaa aataatgaat
     2581 ttactaaaat tactaattct acattaatgc cacctttcac taaggatatg gtagtaaaaa
     2641 ttgaactaaa aaccaaaatg tggaagtgag aatggactca agatccacat aaaatataaa
     2701 tcgataagtt taagtcaacc tttgattatt tttgggagaa aaaaaagccg ttgttataaa
     2761 gggagatggg actggtccag aattggtaaa ctgtatgata gatgttttga aagcctgtaa
     2821 cagcaccata gaacttatac ctgtagaagc tggatccgaa ttttggaaaa aaaataaaac
     2881 aaattcatat atcactgacg aagtttggaa attactatct gaaagtgatg cttgttttaa
     2941 aggtccgact actaccgttc ccgatcctga tgcccctagg agtgtagctg ttagcataag
     3001 acagaaattt gcactttatg caaatattcg gccaataaaa acatataaaa cttcacagtt
     3061 aaatctcaat ttcgtatgtg ttagagaggc aaccgaaggt ttgtatgctg gaatagaatt
     3121 taaaaccagt gacgattctg ctgtggccat tagaaaaata acaagaaaag gatgtgaaag
     3181 ggtagttaaa aaggcatttc aattgagtac ggaattagat tttaagaaaa tatttgttat
     3241 tactaaaaga aacattttaa aagaaaccga tggaatattt gtatctgcag tagagaaaat
     3301 taaaaaggac tttgatacta tagcaacgga agaatactat attgataatg ttactcaaca
     3361 attggtcaaa aatcctcaaa gattcaataa tagtatatta ctaagtacta acttatttat
     3421 ggacataata tctgagtgtg cttcggggca tgttggatca attgggaatg tgtattctgg
     3481 aaattatgga gataaatatg ctatgttcga gcctgctcat gggagtgctc caaaatatgc
     3541 aggtcagaat aaagtaaatc cagtagctac tatttgctcc gctgcatgga tggaagaata
     3601 tttaggtgaa aaagatatct ccaaagctat ttttaaggcc acagaagacg tgatcaatga
     3661 gaataagtat gttacttatg acttaggtgg taatgcatca ctctctaaaa tggctcaaca
     3721 aatttctcaa agagcatcga aaatattaaa aaaatgatac tcattaaaat gactacattc
     3781 agtaaatatt taggaaagac ttatactact taattcatat acataaagag tttcataaaa
     3841 tattttcttc ttattaatta tttttatatt catttttttt tcaaaaatta atctatctaa
     3901 ctttgatata tctgctaaag atgacacaat taatattata tgtccatatt ttttaacatt
     3961 tggtattgca gattgaataa aatgaatagt tttttctatt ccacttatcc ctccatgaat
     4021 agttttatca tcaaattggt cattcggtaa atacggtgga ttacttacaa tcaaatcgaa
     4081 cttactagat agtgctgttg tagcatcgca acatataaga gttatttccg aattactttg
     4141 ttttttacag tataacagag aattgtagtc tatatcagta cttattacat ttttaaaatt
     4201 ttttaagaga aatttagaaa ctattcctgt accggttcca atttcgagtg ctgattgtcc
     4261 agaataatct ttaattgaat ctattaataa aaaactatca acattaggaa tgtacatttt
     4321 caattaattc aattactgct cacatgagtt tagaaagagc tattattttt tctggttcca
     4381 tttcttttac ccttaaatct atttcattaa aattgatttt aagtttatta ttcaatttac
     4441 ttattttttt atttttaaaa gaaaaaatag taaagacctt agaaattgta tccttcgaca
     4501 gtatgttatt ttttgatatt aattttatca cctctgaatc aacggttggt tgaggataaa
     4561 atgattttct atctactttg aatagtggtt gaatgtcaaa acaatattgt attatagctg
     4621 atagtgctct ataattttta tctcctggat tcgattgaat tttttgacta aattcatctt
     4681 gaaccattat aattgcacga gtaaattttt ttgtggctaa ccataaaaat atttctttac
     4741 ttttagaata tgggatattt gaaataaaaa cgtcaaagtt caaaggttta atataattta
     4801 aaagatcttc atttataatt tttagatttt taaattttga ttgaagtaat ttaatttttt
     4861 cgtataactt gtaatctact tcaaaggact ttacgaatct tgcattttta cataaatatg
     4921 gtacaagtat cccttttcct gtacccattt cacatacgtc ttcaaaagct tttatttccg
     4981 atatctctat tatttttctc actacacttg aatcaataag aaaattctgg ccataatatt
     5041 tcctccaatt cattttctga caaagagatt cattcttgtt tctccagaaa tttcttctac
     5101 tattcttttt gtaattaatt tttgtagatc ttttatacca acacgttctt gaatctcatt
     5161 aaaactttca aattttttct tctctctttc tgttataata cttttcatgt atattttacc
     5221 aattcctgga attaattcta atccatggac acgaggagta atcggttgag cattattaaa
     5281 atagtccaca aacctttttt ctttgatagt aactattttc tctattgcta ttggaatttc
     5341 attttttgca gatactgaaa ggtcattata atttaatctt cctaaaacac taattatttt
     5401 atttcttcct tcctttccaa tgtaggcacg ttctccaatt tcaaaatttt gagatggatc
     5461 tccaagtaat tctagtaatg ttaatctctc ttctccaatt gcttgtatta ttatcccttc
     5521 gcgttttttt acggttgagg actttcctct tattgtaaat tcgataatat gagcgtactc
     5581 ttcatatttt cgagtaacgt gaaagctacc aaatgcattt cctttatgat cttgataatg
     5641 atccggtagt gacatcaata ttcatccttt agaatgctct aatgttcttt atttatgata
     5701 tctaaaattt tattcaatgt atctgttaaa atcaattttt tccatccaaa tgtaaaagcc
     5761 cgtaattctt caatagaatt aggcatagca tttgctattt ctactgcttc atcttccttt
     5821 aaatcgcaag atgatacaag ttggttaatt atggtcctgg aaacgtggga aggggattta
     5881 gcaaattttg acacataatc atatgtccat ctctgaattt gatccatttc ttctactcct
     5941 actttttcta aaatatcttt tacttctgaa agggagataa cttctttttt taatgtttcc
     6001 atataagtcc ttcctaatta gttagaggtt ttaaatgatt tcttttagtc tgtaaaattt
     6061 tttctttgtt tcctatagga acggatactt taataattct ttttccaatt tccttaatta
     6121 ttcctatttt tccttgatat cttct
//
LOCUS       DATAFW010000043         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1777512, whole genome shotgun sequence.
ACCESSION   DATAFW010000043 DATAFW010000000
VERSION     DATAFW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777512"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VHJ57_01025"
     CDS             <1..190
                     /locus_tag="VHJ57_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487149.1"
                     /translation="IHLRHMASDYDNHAILSFYPDDMVIFYHSKTGYVIERRKHKPYE
                     LNEIKNAWIHGKITITGA"
     gene            complement(564..637)
                     /locus_tag="VHJ57_01030"
     tRNA            complement(564..637)
                     /locus_tag="VHJ57_01030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Thr,seq:cgt)
     gene            723..2060
                     /locus_tag="VHJ57_01035"
     CDS             723..2060
                     /locus_tag="VHJ57_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487150.1"
                     /translation="MKISISGARGIYGKDLDLHEIYRLSCLFGSYLKKSKDEKIKCLV
                     ACDTRASGDIIKKIVKSSLMEQNIDVYDLNVAPTPIVFRESRKFDGSIIITASHNPFE
                     WNGLKFIIDGRGIFEKELCELLSTQTMFPITSYGVSRNFLSNYENDILELIQKSKELN
                     SYHNKRIGIDAGGGAASGYANNILKNIGHVVYDVNGNKGIFSRGPDPTVDDLLQLKSI
                     VKSNDLDYGFAFDLDGDRLVVVNSNGEKLNSDITLLLCIANMLYNFQGKKFVTSIDTS
                     ISIQNFVKNHGCYLTYSKVGEANVVQKMIEKNANAGGEGSSAGFIMPNFNMCRDAILA
                     SVLISTINKKILKECFEISDNYSQIRTKIQIGSKLQKTILEKLYDEFKKDSTEINILD
                     GIKIIIDENSWILFRISNTEHVLRISVESTKSKINTLYRNVNKRIIKLHDQIK"
     gene            2044..>2727
                     /locus_tag="VHJ57_01040"
     CDS             2044..>2727
                     /locus_tag="VHJ57_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX2487151.1"
                     /translation="MTKLNEKEIIDLIYSNLLEKNKTQFQIRDDVSVLSFKDLKKNKV
                     RPSNSSNIIIKCDMLVESTDVVKQMKIWQIARKSIVSAVSDMSAKGIKPPYFSLLSLA
                     IPSSWTKNKIKNLILGFEKASKEFGIVFLGGDTNESKELIIDCTLIGFLDSTSNSIPL
                     RNNAKAGDIVITSGEFGYTAAGLKILLFNSKAIPSFKKQAISRILLPKPNQKFGTLLG
                     KYFSSSIDSS"
BASE COUNT         1053 a          326 c          418 g          930 t
ORIGIN      
        1 tatacattta agacatatgg catcagatta tgataatcat gcaatactat ctttttatcc
       61 tgatgatatg gttatttttt atcattctaa aactggatat gttattgaac gaaggaaaca
      121 taaaccttat gaactaaatg aaattaaaaa tgcatggatt catggaaaaa taacaatcac
      181 aggtgcataa attcttctct aatgatatat atgatatatt taaacatgat attacttgtt
      241 agttttgatg gactaaacta tttctttcaa atcttataat atattagaaa cttaatccat
      301 atatgtaact tttactactt tttaagtatc ttaatttcaa ttgtaattaa tagtttacat
      361 aaaaaatgta ttatagcaat tattacttgg ttttgaatac gatattatta caattgcaaa
      421 tcctatgtaa gttccaaaat ttgaaatcga ctataatagg tttgaaagtt gctcaataaa
      481 aaaataaaaa gcatattcct gtattcgtat aacaatttta aatttattaa agaataatac
      541 tcaaggtgag aaatcaaaat taaagcctca ggtgggattt gaacccacga cctaaggttt
      601 acgaaacctt cgctctaccg agctgagcta cagaggcagt attaacaaac gaaatttatt
      661 tttactgata tataatttag attctagtaa cacaattaaa tcaaaacttt ctaatcttat
      721 tgttgaaaat atcaatttct ggagcgagag gtatttatgg caaagatttg gatcttcatg
      781 aaatttatag attatcttgt ctttttggat cttatcttaa aaaatctaaa gatgaaaaga
      841 taaaatgtct agttgcttgt gatactcgag cttcaggtga catcataaaa aaaattgtaa
      901 aatcaagttt aatggaacag aatattgatg tttatgattt aaatgtagca cctaccccaa
      961 tagtatttag agagtcccga aaatttgatg ggtctatcat tataactgca tctcataacc
     1021 cttttgagtg gaatggactt aaattcataa ttgatgggcg aggaatattt gaaaaagaat
     1081 tgtgtgaatt attatcaact caaacaatgt ttccaattac gtcgtatgga gtttctagga
     1141 atttcttatc taattatgaa aatgatatac tagaattaat tcaaaagtca aaagaactga
     1201 attcatacca taacaaacga attggtatag atgctggagg tggtgctgca tctggatatg
     1261 ctaacaatat actaaagaat attggacatg ttgtttatga tgttaatgga aataaaggta
     1321 tattctctag aggccccgat cctacagtag atgatttatt acaattgaaa tctattgtaa
     1381 aatcaaatga tcttgattat ggatttgcat ttgatctaga tggagatcgt ttagttgttg
     1441 ttaatagtaa tggtgaaaag ttaaattcag atattacact tttactgtgt atagcaaata
     1501 tgctatataa tttccaagga aaaaaatttg taacaagcat agatacaagt atatctattc
     1561 aaaattttgt taaaaatcat ggatgttatt taacttattc taaggtaggt gaagcaaatg
     1621 ttgttcaaaa aatgatagaa aagaatgcta atgcaggagg agagggaagt agtgcaggat
     1681 ttattatgcc taattttaat atgtgtagag atgctatatt agctagtgtt ctaatcagta
     1741 caattaataa aaaaatatta aaagaatgtt ttgaaatttc cgataattat tctcaaatta
     1801 gaactaaaat tcaaattggg tctaaattac aaaaaactat tttggaaaag ttatatgacg
     1861 aatttaaaaa agattcaaca gaaattaata tcctagatgg gattaaaata attatagatg
     1921 agaattcttg gattttgttt aggatatcta acactgaaca tgttttaaga atatcagtag
     1981 aatctactaa gagtaaaatt aatactcttt atagaaatgt aaataaaaga ataattaaat
     2041 tgcatgacca aattaaatga gaaagaaatt atagatttaa tttattctaa ccttctagaa
     2101 aaaaataaaa cacaattcca aataagagat gatgtatcag tattatcttt caaagattta
     2161 aaaaaaaata aagtaagacc ttccaattcc tctaatataa ttataaagtg tgatatgtta
     2221 gtagaaagta ccgatgtagt taaacaaatg aagatatggc aaatagcaag aaaaagtatt
     2281 gtttctgctg ttagcgatat gtctgcaaaa ggaataaaac caccatattt ttcattacta
     2341 tcattagcga ttcctagctc ttggacaaaa aataaaataa aaaacctaat tttgggattc
     2401 gaaaaagcct ctaaggaatt tggtatagtt tttcttggag gtgataccaa tgaatctaag
     2461 gaactaataa ttgattgtac tttaattggt tttttggatt cgacatctaa cagtattcct
     2521 ttgagaaata atgctaaagc tggagatatt gtaataacgt caggagaatt tggttataca
     2581 gcggctggat taaaaatatt gcttttcaat tcaaaagcaa taccttcttt taaaaaacaa
     2641 gctatatcac gtattctact tccaaaacct aatcaaaaat ttggaacatt attgggaaag
     2701 tatttttcat catcaattga ttctagt
//
LOCUS       DATAFW010000044         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_16456, whole genome shotgun sequence.
ACCESSION   DATAFW010000044 DATAFW010000000
VERSION     DATAFW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16456"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(745..1308)
                     /locus_tag="VHJ57_01045"
     CDS             complement(745..1308)
                     /locus_tag="VHJ57_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HEX2487152.1"
                     /translation="MPQTKPIVSIENVVASATINQTVNLNQITQIFPDVEYHPDQFPG
                     LVFRLKSPKTATLIFSSGKMVCTGAKSEDQAIKAVKSVVQKLKKGSIQIENEPIIEIQ
                     NIVASASLGGKIHLELAARVLPRSMYEPEQFPGLIHRMLDPKTVILLFASGKLVCTGA
                     KKEAEVYRAVHNLHNLLEEKNLMIYEG"
     gene            1447..2832
                     /locus_tag="VHJ57_01050"
     CDS             1447..2832
                     /locus_tag="VHJ57_01050"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX2487153.1"
                     /translation="MSFRIDKDSLGEFKVPKNAYYGPFTARALEQYKVTGRKSHLNLI
                     KAYVMIKRSAALTNYELQVLDERIAKVIIQACDETLSGQFLDQFVIDAINSGAGTAVN
                     MNTNEIIANRALEILRKSKGDYSIISPNDHVNMSQSSNDTFPTAMHIAILMNVNLLIK
                     SLEVLIKSLNIKSIDFKNIIKIGRTHLMDAIPVTLGSEFEVYAYALERAKEEIINSQR
                     KLEYIGIGATAVGTGINTPKGYREKIVNHLSHISGLNLKMSANLHFFLQSKFDIANFS
                     SELRNLAIELIKMANDLRLMASGPIAGLAEILIPAVHAGSSIMPGKVNPSLAECLNMI
                     CFDIIGKDVSVTMAAQAGQFELNVMLPGMLNNVLDATDMLINFLPIFATNFINGIQAN
                     SERLKQQVEKSPILITLLNPYIGYLTAAEIYKESLRTGIGIRELILGKKLMSEKELVD
                     ALSYERIIGLV"
BASE COUNT         1220 a          452 c          492 g         1150 t
ORIGIN      
        1 tgagaatttc ctttatattt tcatcagata acaaatttaa ctctggataa caatgcagta
       61 aattttcttg tttaatatct aatactacat ctgtggaacc ataaccaagc catatctctg
      121 gcatggatat gattataaag aatgaaaata aatctttttc atagtaagat aaaggaacaa
      181 tatttataca actaacttta tagtggttcc taacataatc taattcttta aagttttgga
      241 atttgactag cgagttgtaa agaaagtaag atttatatta tttcaaggtt aatgcatttt
      301 cctaaaatat ttcatcaaac aaaaataaca ttccaaaata tattcaaact gaaaatctcc
      361 aatttgatat tcatacctga ctcttggtta atttttatat ctacaccata gaaaatgttt
      421 aaatcactaa ttatattaaa aaggaaaatt tagtaactga atcaaaaatt tagttgaaga
      481 aaagttgaaa aaatctttat gcttttaatt tatgaaataa ttactacata tcaatcctta
      541 aaactgcgct attgaaaata ttagaaataa tggagtaata ataaaaaaaa tcaatattat
      601 attgtatgag atacaataga acaatataaa atttttaaca caaatagaat aactttatat
      661 tttattaatt attaacgaga tggcaaatat tatctataaa aatgattgta taaatattga
      721 aaaaaagtaa tagtacaaaa ataattatcc ttcatatatc ataagattct tttcttctaa
      781 taaattatga agattatgca cagcacgata tacttctgct tcttttttag cccctgtaca
      841 aactaactta ccacttgcaa acagaagaat aactgttttg ggatctaaca ttctatgaat
      901 taatccagga aattgttcag gttcatacat actgcgagga agaactctag cagctaattc
      961 taagtgaatt ttacctccta aacttgcaga tgcaactata ttttgtattt caatgatggg
     1021 ttcattttca atttggatac ttcctttttt caacttttgt actacactct tgactgcttt
     1081 aattgcctga tcttctgact tggctcctgt acataccatt tttccagagc taaaaattaa
     1141 agtagcggtt tttggagatt taagtctaaa aacaaggcct ggaaattgat ctggatgata
     1201 ttcaacatct ggaaatattt gtgtgatttg atttaaattg actgtttgat ttatagttgc
     1261 tgaagcgaca acattctcta tacttacaat gggtttagtt tgcggcatac tggtatgcgg
     1321 tttgttgact atataaatat attatataaa cttttagaaa aatgattttt gaaatcagta
     1381 tccagtaaaa ccatttgttt tcaagttaac taatattata taaacaagaa ttttatagtc
     1441 attataatgt catttagaat agataaagat tcattaggag aatttaaagt tcctaaaaat
     1501 gcatattacg gtccatttac agctcgtgca ttagaacagt acaaagtaac aggacgtaaa
     1561 tcccatttaa atcttataaa agcgtatgtt atgattaaga gatcagcagc gttaactaat
     1621 tatgaattac aagtcttaga tgagagaatt gccaaagtta ttatccaagc ttgtgatgaa
     1681 actttatctg gacaattctt agatcaattt gttatagacg cgataaattc tggagctggt
     1741 acagcggtta atatgaatac taatgaaata attgcaaatc gtgccttaga aattctgaga
     1801 aaaagtaaag gtgattatag cattattagt cctaatgacc acgttaatat gtctcaatca
     1861 agtaatgata cgtttccaac cgcaatgcat attgcgatat taatgaacgt taatttgctt
     1921 ataaaatctc ttgaggtatt gattaaatct ttgaatatca aatctattga ttttaaaaat
     1981 attataaaaa ttggtcggac tcatcttatg gacgctattc ctgtaacatt gggctctgaa
     2041 tttgaagtat atgcatatgc cctagagaga gctaaagaag aaattatcaa tagtcaaaga
     2101 aaattagagt atattggaat aggtgcaaca gcagttggaa ctggtatcaa tacacctaaa
     2161 ggctatagag aaaaaattgt taatcatctt tctcacattt ctggcctaaa tttaaaaatg
     2221 tctgcaaacc ttcacttttt ccttcaaagc aaattcgata tagctaattt ttcttcagaa
     2281 ttaagaaact tggcaattga actaataaaa atggccaacg atcttagact tatggcttct
     2341 ggccccatcg ctggcttagc ggaaatactc attccagcag ttcacgcagg ttcttctatc
     2401 atgccaggaa aggtaaatcc ttctttggct gaatgtttaa acatgatttg ttttgatatt
     2461 ataggaaagg atgtttctgt aaccatggct gcacaagcgg gacagtttga attaaatgtg
     2521 atgctgccag gaatgctaaa taatgtatta gatgccactg acatgttaat aaatttcttg
     2581 ccaatttttg caactaattt tattaatgga attcaagcga attctgaaag attaaaacaa
     2641 caagtagaaa aaagtcctat tcttataacc ttattaaatc catatattgg atacttgaca
     2701 gcagcagaaa tatataaaga atcgttaaga actggtatag gaatacgaga actaatttta
     2761 ggaaaaaaat tgatgagtga aaaagaatta gtagatgcat tatcttatga aagaattatc
     2821 ggattagttt aaatcttata tttggataga acaacctctg aatcttttgc acatgtgcag
     2881 attgtttctg attctaattt ccctcaaagt agtaggaaaa tagtttggac agtgcttaaa
     2941 ttgtaatcac aaatagaata aataatgaca agatcgaata tttagagata acactgacaa
     3001 aggtaaaata acatttaaac atttattatg tattatggta tacagctgta tacattagaa
     3061 tcattcatac atttggatat cttgattcca attgttttaa ttataataat gagatatatg
     3121 attttaccta aagattatac tctataaata aaatataagt ttgaaatagc acttagtttc
     3181 tataaattgt atattagata ttatctaatt tcttatattg cagaaattta tttaatagat
     3241 atgcatcctg aggacttaaa taaaatttgg ttttgtgaag attgccaaag tagttttttt
     3301 ttcaattcag atgc
//
LOCUS       DATAFW010000045         4822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1426099, whole genome shotgun sequence.
ACCESSION   DATAFW010000045 DATAFW010000000
VERSION     DATAFW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426099"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..376
                     /locus_tag="VHJ57_01055"
     CDS             236..376
                     /locus_tag="VHJ57_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487154.1"
                     /translation="MRKFGGGVENFSMFNSNKINYYCMVCGTKHDNLMCPKCGSKMKR
                     IG"
     gene            507..911
                     /locus_tag="VHJ57_01060"
     CDS             507..911
                     /locus_tag="VHJ57_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487155.1"
                     /translation="MFRKEIYLPFILSISLFLIIGYSSYNHIVLGEEEDSNDGNDNDN
                     NVREEEKEEKEDINDGNDNDNNVREEEKEEKEEDFEDASLDELFDDEDGNDGNDNDNN
                     VREEEKEEKEEDGKEEEDEVPFILPFTAVPFP"
     gene            1019..1133
                     /locus_tag="VHJ57_01065"
     tRNA            join(1019..1044,1060..1073,1089..1133)
                     /locus_tag="VHJ57_01065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1070..1072,aa:Leu,seq:taa)
     gene            complement(1145..1774)
                     /gene="pdxT"
                     /locus_tag="VHJ57_01070"
     CDS             complement(1145..1774)
                     /gene="pdxT"
                     /locus_tag="VHJ57_01070"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2487156.1"
                     /translation="MSTNIKVGVLGIQGDIEENILSTAEALKKLNLRGTVESVRYSES
                     IAELDGLILPGGESTVIGSLLSIQGKVFQTIEKKIKEGMPVFGTCAGLIMLSKRTYDK
                     VVGETKQKLFGILDVVIERNAFGRQHESFEVDLEIPVLGKEPFRGIFIRSPVVNNIGE
                     SVTILTKLNDKIVAVKQNNIIGTSFHPELSADRRLHELFIKDILEFRKD"
     gene            complement(1771..2742)
                     /gene="pdxS"
                     /locus_tag="VHJ57_01075"
     CDS             complement(1771..2742)
                     /gene="pdxS"
                     /locus_tag="VHJ57_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2487157.1"
                     /translation="MTISLVATEEESNGIKFKNLHDEQLVQGTTLVKRGFAHMQKHGV
                     IMDVTTVEQAQIAEDAGAVAVMVLDKLPYDIRKAGGVARTASIKIIEEIMDEITIPLM
                     AKCRIGHISEAKILEVIGVDMIDESEVLTPADEENHIWKWDYTTPFVNGAKNLGEALR
                     RIEEGSSMIRTKGEPGTGNIAEAVRHIKQVNNDIRKLRSFYFDKDEQELIKFSRELKI
                     SYSLIKETAKLARLPVVNFAAGGISTPADAALVMNLGCDGVFVGSGIFKAEDPEQRAK
                     SIVLATTYYDDPTIIMEAQKLVDEKKSLLGLDTKSLELRMQERGPII"
     gene            complement(2844..3473)
                     /locus_tag="VHJ57_01080"
     CDS             complement(2844..3473)
                     /locus_tag="VHJ57_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487158.1"
                     /translation="MSNNLGDNSHTSFSLNGSIFSLMFIGDQIHVNDNNNFANFLNFG
                     KEKEEDVNFVDFLTNSLIDIISNEGDSNFSLEQVLSNSNITTSNNPNNLKKLLLLNGS
                     WNLDVRNGNIVSFDAKISTINKDGMSPHIYEILNLVVLNSNMPIKLSKNDSTIIEGRA
                     DIITPNNIKNIGLYLILQENSISIILDPKILGNDYNGQPIYGLTNYMQN"
     gene            complement(3644..4003)
                     /locus_tag="VHJ57_01085"
     CDS             complement(3644..4003)
                     /locus_tag="VHJ57_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487159.1"
                     /translation="MISEGSEYNKIYNAICSVITDLKEEGIDIKTKFDKESGFIGIYT
                     SNTSILKRAKNGLNEILELSYSTAEHHPYWNIIYNTSEIAKTTLDSWNENFSLDQIQE
                     MLWRNDEIKNTLNRLEK"
     gene            4308..4463
                     /locus_tag="VHJ57_01090"
     CDS             4308..4463
                     /locus_tag="VHJ57_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487160.1"
                     /translation="MNTTAKKPIEDNETPCDYCGNPIEICMCVCPYCGETNSCECCLY
                     DAVTGGG"
     gene            4514..>4822
                     /locus_tag="VHJ57_01095"
     CDS             4514..>4822
                     /locus_tag="VHJ57_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2487161.1"
                     /translation="MKDKIVNEITWLIGGAQGSGVDSAANIFSKTCAEDGLYIFGKRE
                     YYSNIKGEHSYFTVRVSNKPIRSHKESIDILVSFDAETLFRHAKDVSTDGIIIFNSEL
                     I"
BASE COUNT         1690 a          704 c          675 g         1753 t
ORIGIN      
        1 atgagctatt atatcaaaaa atgtttcaat aattaaaagt agatcaagat ttatttagat
       61 gaaatatata atatagttat tgaataataa aaattttatt ttacaacaat taaaatggat
      121 atcaatatat tttgcaatta gtattgtaat ttcttttata attccatttc cctattcaat
      181 aattgtaata attccagtag tattattttt aagcttttat ataagaagaa gagaaatgag
      241 gaaatttgga ggaggggtag agaatttttc catgtttaat tctaacaaaa ttaactatta
      301 ttgtatggta tgtggaacta agcatgataa tttaatgtgt ccaaaatgcg gttcaaaaat
      361 gaaaagaata ggatagcaat atcacaaaca atctaatgag agtttcctat agaaattatt
      421 tctattaaat ataccagtat tattatctag taatacgtta cttaaagcta atataattta
      481 gaataacatg agtaatggta ctatagttgt ttagaaaaga aatctatctt ccatttatac
      541 taagtatttc actattttta attataggtt attcctccta taatcatatt gtactaggag
      601 aggaagaaga tagtaatgat ggaaatgaca atgacaataa tgtaagagag gaagaaaagg
      661 aagaaaaaga agatattaat gatggaaatg acaatgacaa taatgtaaga gaggaagaaa
      721 aggaagaaaa agaagaggat tttgaagatg cttctctaga tgaattgttt gacgatgagg
      781 atggtaatga tggaaatgat aatgacaata atgtaagaga ggaagaaaag gaagaaaaag
      841 aagaggatgg aaaagaagag gaagatgaag tgccatttat acttccgttt actgcagtac
      901 catttcctta attgttgtca aattaaaatt atctactaaa agatgtaaga agttaggtat
      961 aaaaatcacc tttaaccttg taaagtttaa tatcagggtt atcaaatttg cattataggc
     1021 gggggtcgta gagcccggcc aaataacaaa aatttggaga cgcgggactt aagatctaaa
     1081 aatgggtgat cccgtccttt agaggttcgt gggttcaaat cccacccccc gcatgtgaga
     1141 taaattaatc tttcctaaat tctaaaatat ctttgataaa taattcatgt aaccttctat
     1201 ctgctgaaag ctcaggatga aatgaagttc caataatgtt attttgtttt acggcaacaa
     1261 ttttatcatt gagtttagtt aggatagtta cactctctcc aatattattt actacaggtg
     1321 accttatgaa aattccccta aaaggttcct tacctaacac tggaatttca agatctactt
     1381 cgaaagattc atgttgtctt ccgaatgcat ttctttcaat tacaacatct aatattccaa
     1441 ataatttttg ttttgtctct ccaacaactt tatcatatgt tcgttttgat aacattatca
     1501 aaccagcaca agtaccaaat acaggcatac cttctttgat tttcttctct atagtttgaa
     1561 agactttgcc ttgaatagag agcaaacttc caattacagt actttcacca cctggtaata
     1621 ttaaaccatc taattctgct atggattcag aataacgaac cgactccact gttcctcgta
     1681 agtttagctt ctttaatgct tcagctgttg ataatatatt ttcttctata tctccttgaa
     1741 tccctagtac tcctacctta atattggtgc tcaaattata ggacctcttt cttgcatacg
     1801 taattcaaga cttttagtat ctagacctaa taatgatttt ttctcatcta ctaatttttg
     1861 agcttccata attatcgtag gatcatcata atatgttgta gctaacacta ttgatttagc
     1921 tctttgttct ggatcttcag ctttaaagat accagagcca acgaaaacac catcgcatcc
     1981 taaattcata actaatgccg catcggcagg tgttgatatc ccacccgcag caaaatttac
     2041 tactggtaat ctagctaatt tagcagtttc cttaattaat gaataagaaa ttttgagttc
     2101 acgagaaaat ttaattaatt cttgttcgtc cttgtcaaaa taaaaagatc gaagtttcct
     2161 gatatcatta ttcacttgtt taatatgtcg tacagcttct gcaatatttc cagttcctgg
     2221 ctcaccttta gttctaatca tagaacttcc ttcttctatt cttcgtagtg cctcacctag
     2281 attcttagca ccatttacaa aaggagtagt atagtcccat ttccatatat gattctcctc
     2341 atcggcaggt gtaaggactt cactttcatc aatcatatca acacctatta cttcaagtat
     2401 ttttgcctct gatatatgcc caattctaca ttttgccata agtggtattg taatttcatc
     2461 cattatttct tcaataattt taatacttgc ggtcctagcc actcctcccg cctttctaat
     2521 atcataaggt aatttatcta aaaccatgac tgctacagca cctgcatctt ctgcgatttg
     2581 tgcctgttct acagtagtaa catccataat aacaccatgt ttttgcatat gagcaaaacc
     2641 tctctttact aatgtagttc cttgaactaa ttgttcatca tgtaaattct taaacttgat
     2701 gccgttactc tcttcctctg ttgcaacaag agaaattgtc attatttaaa ttattaaaaa
     2761 gcatctatta taggtttggt tagtatgaaa taatatgtaa agttaaacag taaccatatt
     2821 ggacaaataa ttggttaaat atactaattt tgcatataat tggttaatcc ataaataggt
     2881 tgtccattgt agtcatttcc taatattttg ggatctaaaa taatacttat tgaattttct
     2941 tgcaaaatta aataaagacc gatattttta atattattag gagttataat atctgctcta
     3001 ccttctataa tagtactatc atttttagat aattttatag gcatattact atttagaact
     3061 actagattga gaatttcata gatatgtgga gacattccat ctttatttat tgtagatatt
     3121 ttagcatcaa atgatacaat attcccattt cttacatcta agttccaact accattaaga
     3181 aggagtagtt tttttaaatt attaggatta ttagatgtag taatatttga attcgaaagt
     3241 acttgttcta aagaaaaatt tgaatcgcct tcattagaga taatatcaat taaactattt
     3301 gttagaaaat ctacaaaatt tacatcttct tctttttctt tgccgaaatt taaaaaattt
     3361 gcaaaattgt tattatcatt cacatgaatt tggtctccaa tgaacatcaa agaaaatata
     3421 gaaccattta gagaaaagga agtatgagaa ttatctccta aattattaga caaatttaaa
     3481 attggaatag attctccaaa atagagaaat ttgaatatta ctaaaaaaat taaagtaatt
     3541 acaaataaaa ttataagcca tttcattaaa cgactcttta tattttttaa tactattaaa
     3601 tttatatgat tattgctacc aataacaaac taaaagatga atcttatttt tctaatctat
     3661 ttagagtatt tttaatttca tcatttctcc aaagcatttc ttgtatctgg tcaagactaa
     3721 aattttcatt ccaactatcg agtgtagttt ttgctatttc tgaagtatta tatattatat
     3781 tccaataagg atgatgctct gctgtagagt atgataactc taatatttca tttaaaccat
     3841 ttttagctct ctttaaaata gaggtattag atgtatatat tccaataaat ccagattcct
     3901 tatcaaattt agtttttata tctattcctt cttcttttag atctgttata acagaacaaa
     3961 tagcattgta aattttatta tattctgaac cttcagaaat cacatttaat attatattgt
     4021 gttacttatt tatagattag aaattatgag aaatagttat tatattatac aattttaata
     4081 tcaaatagca ttgtaaattt ttatattatt cctcatccaa attatctatg ctttcaaatt
     4141 catcgtaggt ttctttcaag tcctcttcaa attcggtttc aagttcttca ttagacatta
     4201 tatttactta ttataattaa aaaataaatc ttttcatgat atattaaata gtgaaattaa
     4261 ttcatataaa taattattta taagaaggta tatgattata ttaaaaaatg aataccacag
     4321 ccaaaaagcc tattgaggat aatgaaactc catgtgatta ttgtggaaat cccatagaaa
     4381 tatgtatgtg tgtatgccca tattgtggag aaactaattc ttgtgaatgc tgtttatatg
     4441 atgctgttac tggtggtgga taaattctat ttttaagata tttattagat aaaatcacca
     4501 tataaattat agaatgaaag ataaaattgt taatgagatc acttggttaa taggtggagc
     4561 tcaaggaagt ggtgttgatt ctgctgctaa tatcttttct aaaacttgtg cagaagatgg
     4621 attatatatt tttggaaaaa gagaatacta ctcaaatata aaaggagagc atagctattt
     4681 tactgttaga gtatcaaata aaccaatacg atcacataaa gaatctatag atattctagt
     4741 atcttttgat gccgagactt tatttagaca tgcaaaagat gtgtctactg atggaatcat
     4801 tatatttaat tctgaattga tt
//
LOCUS       DATAFW010000046         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3365568, whole genome shotgun sequence.
ACCESSION   DATAFW010000046 DATAFW010000000
VERSION     DATAFW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3365568"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..344
                     /locus_tag="VHJ57_01100"
     CDS             195..344
                     /locus_tag="VHJ57_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487162.1"
                     /translation="MDFKTCGYCGKQFYDIGWPHIEGDSNRGVLKIEHFCCEEHKDSF
                     LKSKE"
     gene            complement(378..716)
                     /locus_tag="VHJ57_01105"
     CDS             complement(378..716)
                     /locus_tag="VHJ57_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487163.1"
                     /translation="MIEKVNSKEWVIGSSMFNANSGKSSNNKKKFPKINLGIRQTVLE
                     RARYRCQVCSIKFNTQETPFFAHINGSLKDNSPANLKALCKECYVKTGENKIKNNIVG
                     KIRSLIRRVI"
     gene            complement(735..1121)
                     /locus_tag="VHJ57_01110"
     CDS             complement(735..1121)
                     /locus_tag="VHJ57_01110"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX2487164.1"
                     /translation="MLNNLIAIVVISFIIVGSLWGIGNLLNPEKENDLDSEGILLIAE
                     NNFFNNTNPTLHINRNIPERITIVNKDFVRHDFIVDGLNVNTGYLYSGQDFTTGIASR
                     ELGTFEYYCSLHPSTMRGEILVADNK"
     gene            complement(1196..2035)
                     /locus_tag="VHJ57_01115"
     CDS             complement(1196..2035)
                     /locus_tag="VHJ57_01115"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX2487165.1"
                     /translation="MKKKISIIGCGAIGSELAQHVDSNMTKNVTLLSLLDIQSENAQT
                     LKSKLSNSSPLIFNNFADFVESESFKDIELVVEAASQNAVTSYLNQLILFKKDVLVMS
                     VGAFANSEFFSEVIKNVENNNINIYVPSGAIAGIDALKSVKNSISYVTLTTTKNPKSL
                     KDSPFFKQNNLTVDSIKKRTLIFEGSASEAVKNFPANVNVAALLGLAGIGVDKTKVNV
                     IADPSIRINKHEIKVIGKFGELIVRVKNIPSSTNPKTSYLAILSVIECLRSITTKGLK
                     YGT"
     gene            complement(2215..>3010)
                     /gene="nadA"
                     /locus_tag="VHJ57_01120"
     CDS             complement(2215..>3010)
                     /gene="nadA"
                     /locus_tag="VHJ57_01120"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX2487166.1"
                     /translation="TNAKNIIFCGVHFMAETASIICPDKDVFIPDLNAGCSLASSINC
                     SELIKWKKDHPDAIVVSYVNTSAEVKALSDYCCTSSNAVKVVNSISPDNEILFLPDMF
                     LGSYVSEITKRKNMFIWPGECHVHAGIRAEDINKMMKDYRNSEFLVHPECSCTSSTMY
                     HMSKGDLLNEGHILSTEGMMARAKNSSKTDFIVATETGILYRMQKENPTKNFIPIRKD
                     AVCQYMKKITIDKVYNSLVNNVYQVRVPKSTADKARYAIERMLAIG"
BASE COUNT         1017 a          473 c          419 g         1101 t
ORIGIN      
        1 aatttttcgg cttcttcatt agttattggg aaacgctcaa gattgagatt cttttcaccc
       61 attttttcta aaagtacttt cttacattga tcacaaatta gctgaagtag tataggcata
      121 agaaataata tataatatca attatattaa ttattaaaaa aagatattaa tttaatgaac
      181 aggaaatact ttaaatggat tttaagactt gcggctattg tggaaaacaa ttctacgata
      241 taggatggcc ccatattgaa ggagattcta atagaggtgt tcttaaaata gaacattttt
      301 gctgtgaaga acataaagat tcatttctta aatcaaaaga ataaatatta ataatattat
      361 ttcagtaata aaatttgtta gataactctt ctaattaatg accgaatttt acctactata
      421 ttatttttaa ttttattttc tccggttttt acataacact ctttgcaaag agctttaagg
      481 ttggcaggac tattatcttt taatgatccg ttaatatggg caaaaaatgg agtctcttgg
      541 gtgttaaatt taatggagca aacttgacag cgatacctag ctctttctag tactgtttgt
      601 ctgattccta gattaatttt aggaaatttt tttttattat tgctactttt tccagagttg
      661 gcattaaaca ttgaagatcc tattacccat tccttagaat taactttttc gatcaattca
      721 ctaaagaaag ataattattt gttatcagca acaagtattt ctccacgcat tgtagatgga
      781 tgcaacgaac aataatattc aaaagttcct aattctcttg aagcaatacc tgtggtaaaa
      841 tcttgacctg agtaaagata acctgtatta acgtttaaac catctacaat aaaatcgtgt
      901 cttacaaaat ctttatttac aatagtaatt ctttctggta tatttctatt aatatgaagt
      961 gtaggatttg tattgttgaa aaaattattt tctgcaatta ataaaatccc ttctgaatct
     1021 aaatcattct ctttttctgg attgaggaga ttaccaattc cccataacga acctacaata
     1081 ataaaagaaa ttacaactat tgcaattaaa ttatttaaca tatttcttat cttttactat
     1141 ccaaaattta cgttattgag tccataaaaa cagtgaattg gtatattaac gacttttatg
     1201 ttccgtattt taatcctttt gtagtaattg agcgaagaca ttcgataaca gataatatcg
     1261 ccaagtaact tgttttagga ttagtcgaac taggaatatt tttgactctt actattaatt
     1321 cgccaaattt tccaatcact ttaatttcat gtttattaat acgaatagat ggatcagcaa
     1381 ttacattaac cttggttttg tcaacaccta tcccagccag acctaacaag gctgctacat
     1441 ttacattagc tggaaaattc ttaactgcct cacttgctga cccttcaaaa attaaggttc
     1501 tttttttgat agaatctact gtcaaattat tctgcttaaa aaagggagaa tctttaagtg
     1561 attttggatt cttagtggtt gtaagagtta cataacttat tgaatttttt acactcttga
     1621 gagcatctat tccagcaata gccccagaag gaacataaat atttatatta ttattctcta
     1681 cattttttat cacttcagaa aagaactcgg aattagcaaa agcacctaca ctcataacaa
     1741 gtacatcttt cttaaataaa atgagttgat taagataact tgttacagca ttttgtgaag
     1801 cagcctctac taccaactct atatctttaa atgattcaga ctcgacaaag tcagcaaaat
     1861 tattaaagat tagaggactg ctatttgata attttgattt aagggtttgt gcattctcag
     1921 attggatatc aagaagagaa agtaaagtta catttttggt catattacta tctacatgtt
     1981 gggcaagctc acttcctata gcaccacaac ctatgattga tatctttttt ttcataaaaa
     2041 gtaattaaag actttattgt atgagttaaa atagtttcct aaagtaaatt ttaatatagt
     2101 tatatttaaa aaataataga tcaattaaat tgctctgtgt taaaataatt tagttgtaag
     2161 ataatagtca agagagtaac ttatatttta aaagagaatc aaaatacaaa actatcatcc
     2221 tattgctaac attcgctcta ttgcatatct ggctttatca gctgttgatt tgggtactct
     2281 tacttgatat acattattta ctaaagaatt gtatacttta tcgatggtaa tttttttcat
     2341 atattgacat actgcatctt ttctaatagg aataaaattt tttgtagggt tttctttttg
     2401 catcctatat aatattcctg tttctgtagc tacaatgaaa tcagttttag atgaattttt
     2461 cgctcgtgcc atcatacctt ccgtagataa aatatgacct tcgtttaaca gatctccttt
     2521 tgacatatgg tacatcgttg aagatgtaca actgcattct ggatggacca gaaattctga
     2581 atttctgtaa tccttcatca ttttattaat gtcttcagca cgtattccag catgtacatg
     2641 acattcgcct ggccatatga acatgttctt tcttttggta atttcagata catatgaacc
     2701 taagaacata tctggaagaa agagaatttc attatctgga gatatgctat ttactacttt
     2761 tacagcatta gacgaagtac aacaatagtc tgataacgct ttcacctctg cagaagtgtt
     2821 cacataacta acaacaatcg catctggatg atctttcttc cactttatca attctgaaca
     2881 atttatagag gaagcaagag aacatcctgc atttagatcc ggtataaata catctttatc
     2941 tggacaaata atagatgcag tttccgccat gaaatgaaca ccacaaaaaa taatattttt
     3001 agcattagtt
//
LOCUS       DATAFW010000047         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1253373, whole genome shotgun sequence.
ACCESSION   DATAFW010000047 DATAFW010000000
VERSION     DATAFW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253373"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VHJ57_01125"
     CDS             complement(<1..274)
                     /locus_tag="VHJ57_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487167.1"
                     /translation="MDIFSEKVHDYLVTPNFKLVMLVNMLCIVSGLNLISDYDYSFAQ
                     INASSPSSLSNNSQQIQESLGSTNRDAILSYEQGNKFLNAKNYDQAI"
     gene            complement(376..1923)
                     /locus_tag="VHJ57_01130"
     CDS             complement(376..1923)
                     /locus_tag="VHJ57_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2487168.1"
                     /translation="MSNKQADNNNNNSGSNADNIEFPTSKKNLILNNNTSDKSIHNIN
                     NNLTFISEFIRRKSGIAGVIILLILVSMTIYAFFGIPSASFKEWNNPNYWIDNPRLAS
                     PIWSDFFGYLGRDTPEHLILSSSVENNDANRGANKVTISTTNENGINVVTHSYHINYN
                     SDIPPNDFMITYSLHKEKIPPAIEIELIRPNNNKFDIYFDSITPSISGSNQSITLGRI
                     FSTDSLIHQKLLDYLKFYNYKQETSKPEVMLFSNHENRSILKGNYTFNVKFYLFNNED
                     EILNSKLILGGEKFGLMGTDELRRDLTVGLVWGAPVALFIGLSVSTISIVIGMIYGII
                     AGYKGKRTDESLMRINDIFYSLPTLPLLIIMSLFLGRSIFLIVLFLIFFGWMGTAKIS
                     RSLALQIKNFQYVEAAKLIGQSDIKIIFKHIIPQLLPLTFASIAISVPGAILAEASLS
                     FIGLGDPSIPTWGQILHEAHTAAAASRGLWWWLIPPGMLIALTGLAFVLIGNTIDSLL
                     NPKMKRM"
     gene            complement(1916..2866)
                     /locus_tag="VHJ57_01135"
     CDS             complement(1916..2866)
                     /locus_tag="VHJ57_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2487169.1"
                     /translation="MDNVIKDSVKYNVIDGLSNQKVEFKDITERQRYIDKQILLQIKN
                     LGLEEPWYSPNRFFNTLFKVMVLDLGKSNFFVSDSGSSSVKDIIIEKIPKTLLLFTTS
                     TIVIALMGVYLGVFLASRADSKLDRINSIFAIFSNSIPVWWFGMIMIFIFAFSLNIFP
                     ARSTPSTPPNDPFYFFNLLYHMTLPLITMVILGFGSVAFIVRHIVSGILEEDFIKAKF
                     AMGIPKKKILYSHALKNAAPPIITMIALSLAGSFGGAFTVEVVFDWPGMGKLYFDAIG
                     LMDVPIIIGLTYVSTLIFLVTVFFIDIIYAYFDPRVKLNE"
BASE COUNT         1110 a          465 c          466 g         1063 t
ORIGIN      
        1 taatagcttg atcatagttt ttggcattca aaaatttatt tccttgttca tacgacaata
       61 tagcgtctct atttgttgat cctagagatt cttgtatttg ttgactatta ttggatagag
      121 aggaaggaga agacgcattt atttgtgcaa atgaataatc ataatcacta attaaattta
      181 atccagaaac tatacataac atattaacta acataacaag tttaaaattt ggcgttacta
      241 aataatcatg gaccttttca ctaaatatat ccattcatta ttgtaagatt ttcacacata
      301 aatttttttt tatgattgtt atgcttaaaa acagcaacat aatgataatt aaaagaagat
      361 aaatatattt tgtaattaca ttcttttcat cttgggattc aagagagagt ctattgtatt
      421 tccaatcaga acaaaggcta gccctgttaa agcaattaac atgccaggag ggattaacca
      481 ccaccatagg cctcttgatg cagctgctgc ggtatgagct tcatgaagaa tttgacccca
      541 tgttggaata gatggatcgc ctaaaccaat gaagcttaga gaagcctctg cgagaattgc
      601 tcctggaacg gagatcgcaa tgcttgcaaa agttaaagga agtaattgag gaattatatg
      661 tttaaagatt attttaatgt cagattgtcc aataagcttt gctgcttcaa catattgaaa
      721 attctttatc tgtaatgcta gacttcgtga aatcttagca gttcccatcc atccaaaaaa
      781 tattaaaaat aaaacaataa gaaatatact tcttcctaaa aacaaggaca taataatcaa
      841 aagcggtaga gttggtaaag aatagaaaat atcattaatt ctcattagac tttcatctgt
      901 tctttttcct ttgtagccag caataatacc atatatcatc cctattacaa tggaaattgt
      961 ggaaacagat aaacctataa ataaagcaac aggtgctccc cacaccaatc caacagttaa
     1021 atctctacgt aattcgtctg tgcccattaa tccaaatttt tctcctccaa gaattaattt
     1081 agaatttaga atttcatctt cattattgaa gagataaaat ttaacattga aagtgtaatt
     1141 tcctttcaaa attgatcgat tttcatgatt tgaaaatagc attacttccg gttttgaagt
     1201 ttcttgttta taattataaa attttaagta atcaagtaat ttttgatgta ttagactatc
     1261 tgtggaaaat attcttccca atgttatgct ttgatttgaa ccagatattg atggagtaat
     1321 tgaatcgaaa taaatatcaa atttattgtt gtttggtctt attaattcta tttcaatggc
     1381 aggaggaatt ttttctttat gtagtgaata ggttatcata aaatcatttg gtggaatatc
     1441 agaattataa ttaatatgat atgaatgtgt aacgacatta atcccatttt catttgtggt
     1501 agatattgtt accttattcg cgcctctatt cgcgtcatta ttttctacgg aagaagaaag
     1561 aatcagatgt tctggggtgt ctcttccgag atatccaaaa aaatcactcc atataggtga
     1621 tgcaagccta ggattatcaa tccaataatt ggggttgttc cattctttaa aagatgcaga
     1681 tggtatacca aagaaagcat aaatggtcat agataccaaa attaaaagaa taattactcc
     1741 tgcaataccg cttttccttc gtataaattc agatataaaa gttaaattgt tatttatgtt
     1801 gtgaatagat ttatcactcg tgttattatt tagtattaaa ttcttcttcg aagtaggaaa
     1861 ttctatattg tcggcattag aaccggaatt attgttattg ttatcagctt gtttattact
     1921 catttaattt caccctagga tcaaagtacg cataaattat atctataaaa aaaactgtta
     1981 caagaaatat caaggtagag acataagtaa ggccgataat aataggaaca tccatcaatc
     2041 caatagcatc aaaatataat tttcccattc ctggccaatc aaagaccact tcaacagtaa
     2101 aagctccacc aaatgatcca gctagactta gagctatcat tgtaattata ggaggagctg
     2161 catttttgag agcatgcgaa taaagtattt tctttttagg aatacccata gcaaattttg
     2221 ctttgataaa gtcttcttct aatattccag atactatatg tctaacaata aatgcaacag
     2281 atccgaatcc aagaattacc attgtgataa gtggaagggt catatgatat agtaaattaa
     2341 aaaaatagaa tggatcattt ggaggagttg atggtgttga acgagccggg aatatattaa
     2401 gcgagaacgc aaaaataaat atcattatca ttccgaacca ccaaacggga atactattac
     2461 tgaaaatagc aaagatcgaa ttgatcctat ccaatttgga gtcagcacga ctagctaaaa
     2521 aaactcccaa ataaacaccc attaaggcaa taactattgt tgatgttgta aaaagtaaaa
     2581 gagttttagg aattttttca attattatat cttttacaga tgatgatcct gaatcactta
     2641 cgaagaaatt cgattttcca aggtctaaca ccataacttt gaataaagta ttaaaaaatc
     2701 gatttggaga ataccatggt tcttctaacc ctaaattttt tatctgcaag agaatctgtt
     2761 tgtcgatata tctttggcgt tctgtaatat ccttaaactc tactttttga ttactcagac
     2821 catctataac attgtatttc acggaatcct taattacatt atccattgtt gggcccaaaa
     2881 gagatatagt tagaactaga attgaaaata aaactataaa catattgatg attcttttaa
     2941 ataaaaaaaa caagaatcct gatgtcattt atattaaaag tagtgatatt aaatatatta
     3001 atagtattgt ttaatcaata tttattttat atgtagtttg tataaacagt tatttgttta
     3061 taaaaattct atttactaat tatttttgga attattaatt tgta
//
LOCUS       DATAFW010000048         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1780757, whole genome shotgun sequence.
ACCESSION   DATAFW010000048 DATAFW010000000
VERSION     DATAFW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780757"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VHJ57_01140"
     CDS             complement(<1..1254)
                     /locus_tag="VHJ57_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX2487170.1"
                     /translation="MLDEYNKKRNFDLTPEPPGNKKQLTDHKSSSKFVVQKHDASRLH
                     YDFRLEDTKQRVLKSWAVPKGISLNPKIKRLAVLTEDHPLDYLLFEGVIPQGSYGAGT
                     VIVWDTGTYTSEQEISDQFQKGKITFTLFGHKLKGKFTLVRTHLGREKEGKQWLLIKL
                     ADEFESNEELTITRPNSVLTSRINKDLEKENRPYNKKDKTKVSFNKPTKMSDNNQPKY
                     TNVFSNQQQGEEVVEEFPTTIKPMLSTLVDEPFNSKDWLFEVKWDGVRSILFFNKLKK
                     TLELQSRNGRFITHRYPELVTAFKLAIKCKESAILDGEIVVLDKKTGIPNFQNHQRRM
                     NIDSSREIEILSKDLPATYYFFDILYLDKKNLQDLPFLERRKILSEVIIENTRIKNSD
                     FMEEIGKKIFDSTKRMGLEGLIAKHK"
     gene            complement(1490..1981)
                     /locus_tag="VHJ57_01145"
     CDS             complement(1490..1981)
                     /locus_tag="VHJ57_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487171.1"
                     /translation="MLFQTEKTIATATKPEVESGRKILNECLRNSMNFRNQNVNLAKI
                     ELNSPTKKDDLIYSYQWMTDAEMQLIVNRFIKFSSLLCLASSSTPSSSTNESTDKEIQ
                     QKQQQQPHLIIDVFGQETEPICTYYRCRHKFSLHGLRTHGCRCKHPMNKTLGVFMRYD
                     EEK"
     gene            complement(1999..2841)
                     /locus_tag="VHJ57_01150"
     CDS             complement(1999..2841)
                     /locus_tag="VHJ57_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX2487172.1"
                     /translation="MAARTIWKGSISFGLVNIPVQVFVATQKDEFSSFNQLDEKGHKI
                     KYKKWCPIEEREIPWSEIKKGYEITKDNYVVLEKEDLENIKLKTTHTIEINEFIDSEE
                     FDPLFIEKTYYIGPDTGMTKKKKNTSPPTSKAYSLFVKILNETNKVAIGKVVLRDKEH
                     LVALRSYQRGLIMHQLKYLDEIKPMDEVEGISSVQQPKVEDKELSLGKTLVDNLTNEQ
                     FDIGKYSDTYSKEVKKLIEAKSKGQKVTIGKEEDKAEDTTNLLEALKASIDLKSKPHR
                     QKKN"
     gene            3125..3334
                     /locus_tag="VHJ57_01155"
     CDS             3125..3334
                     /locus_tag="VHJ57_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487173.1"
                     /translation="MNLNNTNQLSSYIRGFESDSPESLGEKITTFLTENPELELKDIK
                     YSSYGFIHNLNRETSYSALVISIRK"
BASE COUNT         1175 a          582 c          533 g         1505 t
ORIGIN      
        1 tttatgtttg gctattaatc cttctaatcc cattcgttta gttgaatcga aaattttttt
       61 acctatttct tccataaaat cggaattttt tatccttgta ttttctataa taacctctga
      121 aagaattttt cgtctttcta aaaatggtaa atcctgcaaa ttttttttat ctagataaag
      181 aatatcaaaa aagtaatagg ttgctggtag atctttagat aaaatttcga tttctctact
      241 ggaatcaata ttcattctcc tttgatgatt ctgaaaattc ggaatccccg tttttttgtc
      301 caagacaaca atctcaccat ccaaaattgc tgattcctta cattttatag ctaacttgaa
      361 tgccgttact agttccggat atctgtgagt gataaatctg ccatttctag attgtaattc
      421 tagtgttttc tttaacttat taaagaatag tatagaacgt actccatccc atttgacttc
      481 aaagagccag tctttgctgt taaagggctc atctactaat gttgacagca ttggttttat
      541 tgtggttgga aactcttcta ctacttcttc tccttgttgt tggttagaaa aaacattagt
      601 atattttggc tgattattat cactcatttt ggttggttta ttaaaagaga ctttggtctt
      661 gtcttttttg ttgtaaggtc tattttcttt ttctaaatcc ttatttatcc tgctggttaa
      721 aacagaattt ggtctagtta tagttagttc ttcattagat tcgaattcgt cagctaattt
      781 aatcaagagc cattgctttc cttctttttc tctacctaaa tgagtcctaa ctaaggtaaa
      841 ctttcccttt agtttgtgac caaataaagt aaaagtaatt tttccttttt gaaattgatc
      901 tgatatttcc tgctcggatg tgtatgtacc tgtatcccat acaattacag tacctgcacc
      961 gtaacttcct tgaggaatta caccctcaaa gagcaaataa tctaatggat ggtcttctgt
     1021 tagaactgca agtcttttta tttttggatt aagtgagatt ccttttggta cagcccaaga
     1081 ttttaatact ctttgttttg tgtcttctag cctaaaatca taatgtaatc tgctagcatc
     1141 gtgtttttgt actacaaatt tgctgctgct tttatggtct gtgagctgtt ttttgtttcc
     1201 tggaggctcg ggtgtgagat caaagtttct ttttttatta tattcatcta acattttgtg
     1261 ccactaattt acaatataga agaaaaaacg agaagtagta ttaattatat acgtagtcaa
     1321 caatatcata ccaaaaggta acaaatttct gatagatttt ctatatgttt cttcaatctt
     1381 cttttagcga ttgattctat tactccaatt tctatgtctc attctcaaat gcttaactgc
     1441 aacattttct cttactgaat agttcatgca aatcccttat aataaattat tatttttctt
     1501 catcatacct catgaatact ccaagtgttt tgttcatagg atgtttacat ctacaaccat
     1561 gcgtgcgtag accatgtagt gaaaacttgt gacgacatct atagtaagta catattggtt
     1621 cagtctcttg accgaataca tcaattatta ggtgtggttg ttgttgttgt ttttgctgta
     1681 tttctttgtc tgttgattca tttgttgaag atgatggtgt tgacgatgaa gctaaacata
     1741 ataatgaaga aaacttaata aacctattca caattagctg catttctgca tcagtcatcc
     1801 attgatagct ataaattaaa tcgtcttttt ttgtaggcga atttagctct attttcgcaa
     1861 gatttacatt ctgatttcta aaattcattg aatttcttaa acattcattt agaatctttc
     1921 ttcctgattc tacttctggt tttgttgcgg ttgctattgt tttttctgtc tggaaaagca
     1981 ttaactatct accatcctct aatttttctt ttgtctatgg ggtttgcttt ttagatcaat
     2041 gctagcttta agagcctcaa gtaagtttgt agtatcttca gctttatctt cttctttccc
     2101 gatagtaact ttctgtcctt ttgattttgc ttctattagt ttttttactt ctttagagta
     2161 agtatctgaa tatttaccta tatcaaattg ttcattagtt aagttatcaa ctaatgtctt
     2221 tcctaaagat aactccttat cctcgacctt aggctgctga acgctagaaa taccttcaac
     2281 ttcatccatg ggttttatct cgtctaaata ttttaactga tgcattatga ggcctctttg
     2341 atatgatctt aaagcaacca aatgttcttt atctcttaag accaccttac ctattgctac
     2401 tttattagtc tcattgagaa tcttgacaaa tagagaatag gctttgctag tgggagggga
     2461 tgtatttttc ttcttctttg tcattccagt gtcaggacca atataatagg tcttctcgat
     2521 aaatagagga tcaaattctt ctgaatctat gaattcatta atctctattg tatgtgtagt
     2581 ctttaatttg atattctcta aatcttcttt ttctaaaaca acataattgt cttttgtaat
     2641 ttcgtagcct ttctttattt cagaccaagg aatttccctt tcttctattg ggcaccattt
     2701 tttatattta attttgtgac ctttttcatc taattgatta aaagatgaaa actcatcttt
     2761 ttgtgtagct acaaaaacct gaacaggtat gttgactagt ccaaaagata tactgccttt
     2821 ccatatagtg cgagcagcca tactaatagt ataccctaca atcctttaaa caactatgga
     2881 ataaagatat gtatctttag ttggatgatt attctgaata atcatctaga aaaattttat
     2941 ctatgtaata aatgggaaaa ggcaaaatga agagcctata catcattctc atgattcatt
     3001 aataatataa agaatattat ctcgatattt acatctgcta atctaaattc atagtatatt
     3061 ttaactgctt tttcaggtat tttatagtta atttataagt caacagattc tatttaacat
     3121 taaaatgaat ctaaataata caaatcagtt aagttcgtat attagaggat ttgaaagtga
     3181 ctcacctgaa agtcttggag aaaaaataac tactttttta acagaaaatc cagaattaga
     3241 acttaaagat attaaatata gttcatatgg atttatacac aatcttaata gagaaactag
     3301 ctatagtgca ttagtgattt ctattaggaa ataaaaaact ttattttttt aaattattta
     3361 ataaatttac aatacaaggt attatataat tgataattgt agaatgtgtt gtaatcattc
     3421 attatcattt taatgtaata tttcaaaatg atgaaaatgt atagtaaatt ttttgaatat
     3481 caaatattga gaattgaatc tatttaatat tccattttta aatttattct tattttatta
     3541 aagtaattgt tataaaacta ttaataatgc attaaagtat tcctattcaa attggtaatt
     3601 actaaaaaaa gtaatgaaag atcattgtta caggacacat aaaatatatt tatatctagt
     3661 atttctataa atataaaagg gaaaggaaat gggaataata ataagcaaaa atgatgaatt
     3721 tactacattc ttaaaaaata aacttaagga aaggtacgag caaagaagaa ggttagataa
     3781 taaagtacat gtatc
//
LOCUS       DATAFW010000049         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3014237, whole genome shotgun sequence.
ACCESSION   DATAFW010000049 DATAFW010000000
VERSION     DATAFW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014237"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..294)
                     /locus_tag="VHJ57_01160"
     CDS             complement(10..294)
                     /locus_tag="VHJ57_01160"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX2487174.1"
                     /translation="MNNFKKLSDNEIEIKLKELRNWEIQQGKLCKSFEFKNFTQAFGF
                     ITKIALESEKLDHHPELFNVFNKVKIFLSTHKINGLSEYDFILAKKIDDL"
     gene            445..>1716
                     /gene="tuf"
                     /locus_tag="VHJ57_01165"
     CDS             445..>1716
                     /gene="tuf"
                     /locus_tag="VHJ57_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HEX2487175.1"
                     /translation="MSSSKKPHMNLVVTGHVDNGKSTIVGHLMVDLGVIDQRTIEQFA
                     KESEATGKGDTFKYAWVLDSIKDERDRGITIDLAFQKFETSKFFFTLIDAPGHRDFIK
                     NMITGASEADVAILVISTKPGETEAATEPGGQAREHAFLCRTLGVGQVVVALNKMDDS
                     NYSEARYNEVKGIAEKMLKMVGYNTSKVNFVPVSGWKGDNLAKKSENMGWYKGLTLLE
                     ALDSFESPEKPIGKPLRIPIQDVYSITGVGTVPVGRVETGVVKANDKVIVMPAGVSGE
                     VKSIETHHTQMNSAEAGDNIGFNLRGIDKKSIHRGDVIGHPDKPPSVAKEFEAQIIVI
                     HHPTAMAPGYTPVLHAHTAQVAATLSDFVAKIDPKTGGVLEEKPKFLKTGDAAIVRIR
                     PVRPLAIETFKEFPEIGRFALRDMGTTIAAGV"
BASE COUNT          602 a          255 c          320 g          539 t
ORIGIN      
        1 tatgtattct taaaggtcat caattttctt tgctaatatg aagtcatatt cacttaaccc
       61 attaattttg tgtgtagata aaaaaatttt cactttatta aaaacattaa ataattctgg
      121 atggtgatcc aatttttctg attctaatgc tatcttggta ataaaaccaa aggcttgagt
      181 aaaattttta aattcaaaag atttgcataa cttaccttgt tggatctccc agtttcgtag
      241 ttcttttaac tttatttcaa tttcattatc agaaagtttt ttaaaattat tcaactaaaa
      301 ataattactt gttgaccatt aatgtatttt actataattg ttaaagcaaa ataatttaat
      361 tttttatgtt ctcttgtctt ctcagaagag attcattttt tgataatcga aatataaata
      421 aagcagatga atactcttcc gtgtatgagc tcaagcaaaa aaccacatat gaatctcgtg
      481 gttacaggac atgtagataa tggtaaatct actattgtag gccatttaat ggttgattta
      541 ggagttattg atcaaaggac tatagaacag tttgcaaaag aatccgaagc tactggtaag
      601 ggtgatactt tcaaatatgc atgggtttta gatagtatta aagatgaaag agatcgagga
      661 attaccatag atttagcttt tcagaaattt gaaacttcta agttcttctt tacattaatt
      721 gatgcccctg gacataggga cttcattaaa aatatgataa caggtgcctc agaagctgat
      781 gtggctatat tagttatttc cacaaaacca ggggaaacag aagccgcaac ggaaccggga
      841 ggccaagcta gagaacatgc atttctctgc agaacattag gagtaggaca agtagttgtt
      901 gcattaaata aaatggatga ttccaactat tcagaagcaa gatataatga agtcaaaggt
      961 attgcagaaa aaatgctcaa aatggtaggt tataatacta gtaaagtaaa ttttgtccct
     1021 gtttctggat ggaaaggtga taatttagca aaaaaatctg aaaatatggg ttggtataag
     1081 ggattgactt tattagaagc acttgattct ttcgagtccc cagaaaaacc aattgggaaa
     1141 ccattaagaa ttccaattca agatgtatat tcaattactg gtgtaggtac tgttccagta
     1201 gggagagtag aaacaggtgt tgtcaaagct aatgataagg taatagtcat gcctgcagga
     1261 gtatcgggag aagttaaatc tatagaaact catcataccc agatgaattc agccgaagca
     1321 ggtgataata taggatttaa tttaagagga attgacaaaa aatctattca tagaggagac
     1381 gttataggac atccagataa acctccaagt gttgctaaag aatttgaagc tcaaataata
     1441 gtaatccatc atccaactgc tatggcgcca ggttacactc cagttttaca tgctcataca
     1501 gcacaagtcg ccgcgacttt atcagatttt gttgccaaaa ttgatcctaa aactggcgga
     1561 gttttggaag aaaaaccaaa atttcttaag acaggtgatg cagcaattgt aagaattaga
     1621 ccagtcagac ctcttgctat agaaactttc aaagaattcc ctgaaatcgg tagatttgca
     1681 ttaagagata tgggtactac gattgctgct ggagtt
//
LOCUS       DATAFW010000050         5281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1429425, whole genome shotgun sequence.
ACCESSION   DATAFW010000050 DATAFW010000000
VERSION     DATAFW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429425"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VHJ57_01170"
     CDS             <1..502
                     /locus_tag="VHJ57_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487176.1"
                     /translation="LLFNSKAIPSFKKQAISRILLPKPNQKFGTLLGKYFSSSIDSSD
                     GLGTSLYELAKQSKVDFYVDDIPFPRGLIEFAKSNSLDIYDLIFKGGEEYEILATINP
                     SNLQKVNFLAKKFHLPMFVIGKAMIGSGNVFVKYDNKLELFHGRKIEKGKDYFLIENK
                     GFLHFS"
     gene            549..956
                     /locus_tag="VHJ57_01175"
     CDS             549..956
                     /locus_tag="VHJ57_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX2487177.1"
                     /translation="MSETEDITQYKWGVAHIFSSYNNTLVHITDISGAETISFSSGGR
                     HVTADRYESSPYAAMKSAVSAADTAKSKGINALHIRVRAVGGVGPRIPGPGAQAAIRA
                     LARAGFRIGRIDDVTPVPHDTTRKPGGRRGRRV"
     gene            complement(1151..1555)
                     /locus_tag="VHJ57_01180"
     CDS             complement(1151..1555)
                     /locus_tag="VHJ57_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX2487178.1"
                     /translation="MEEDLDIEILKAKKMQEIMQRMIKQKKENKEKDIKKREPSEREI
                     LRSYLYDRGDEVLKLAESQFPSETRAIINRIVELINSGEINQKISGGELLSLFRTIGI
                     NVKINTTIKIEEHGKYMSFAEKLKQNKEIDDE"
     gene            complement(1556..3028)
                     /locus_tag="VHJ57_01185"
     CDS             complement(1556..3028)
                     /locus_tag="VHJ57_01185"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX2487179.1"
                     /translation="MNKIITKRGNHVIEKLNEFEFKINVDNSKKMNVPVTIYADDSLI
                     LKMSEDRTLDQAVNVSTLPGVRKHVVVLPDGHEGYGFPVGGVAATDMEEGVISPGGVG
                     YDINCGVRLLRSNLHVNQVKPHLKDLVNELFNSIPSGVGSEGSIKLTRSELDELLIEG
                     VDWAIKHGYGYKQDSEVCEEKGRMIGANPESVSDTARKRGAPQLGSLGSGNHFLEIQQ
                     IQEIFDENAANMMGLEKGQITVLIHCGSRGFGHQVCSDYLRYSEKSLKKYNIELIDRE
                     LACVPNNSIEGENYRKAMYSALNFAWSNRQIITHFTRKTFEKIFSSSETGLDMKLVYD
                     VAHNIAKVERHKVDGEGMRDLVIHRKGATRAFPAGMEQVPEKYRQIGQPVIIPGSMGT
                     ASWILVGNKKSLDLSFGSTAHGAGRTMSRGAAKRSYTIEKVKAELDSKGIYIKALTKN
                     GIVEETPLAYKDVDSVVNVSDKLGIATKVAKLVPIGVIKG"
     gene            3808..4425
                     /locus_tag="VHJ57_01190"
     CDS             3808..4425
                     /locus_tag="VHJ57_01190"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX2487180.1"
                     /translation="MFTGIIESLCEIKSINEIKKGNSKYPAEIKFSIDIGNLSKGLKI
                     GQSIAVNGVCLTITKLNNNIAEFSVIGETVRKTCLDSIKKGEKVNIERSMRVSDRFDG
                     HFVQGHVDCTGIIYDKIVFPTETKLIIKIFGELKESFRYVVRKGSITIDGISLTVVDI
                     DNKNLVSISLIPHTLKKTTLGIKSIGDKVNIEFDIIGKYISRLLP"
     gene            4487..5167
                     /gene="ribB"
                     /locus_tag="VHJ57_01195"
     CDS             4487..5167
                     /gene="ribB"
                     /locus_tag="VHJ57_01195"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX2487181.1"
                     /translation="MSTVKDAIDALSQGKFVLIHDDDSRENETDMIIAAENITPYHIA
                     TIRNDAGGLLCLAIDSYISTKLGLSYIHDILNCVSNDHPLFTAMTNGRAPYGDKPSFS
                     ISVNHRDTYTGITDYDRALTISKLASVCKNIDNGGVSEFIKCFRTPGHVSLLIAAKGL
                     LKERFGHTELAVYLAKIAGLTPAVAICEMLDSHTFRSLSIDEAYKYAKLKDIPLLEAD
                     QLKTYSVI"
BASE COUNT         1718 a          849 c          734 g         1980 t
ORIGIN      
        1 attgcttttc aattcaaaag caataccttc ttttaaaaaa caagctatat cacgtattct
       61 acttccaaaa cctaatcaaa aatttggaac attattggga aagtattttt catcatcaat
      121 tgattctagt gatggattag ggacctctct ttatgaattg gctaaacaaa gtaaggttga
      181 tttttatgtt gatgatattc catttcctag aggtctaatt gaatttgcaa aaagtaattc
      241 tctagacatt tatgacctga tatttaaggg tggtgaagaa tacgaaatac tagctacgat
      301 caatccctcg aatttgcaaa aagtaaattt tcttgcaaag aaatttcatt taccaatgtt
      361 tgtaatagga aaagcaatga ttggaagtgg taatgttttt gttaaatatg ataataaact
      421 tgaattattt catggaagaa aaattgaaaa aggtaaagat tattttctaa tagaaaataa
      481 aggattttta catttttctt aatatattaa caaagtttta atatccaaat taagtgaatt
      541 aaaagattat gtctgaaact gaagatatta ctcagtataa atggggagta gctcatattt
      601 ttagtagtta taataataca ttagttcata ttaccgatat tagtggtgct gaaacaatat
      661 cgtttagttc tggaggtaga catgtaactg ctgataggta tgaatcatca ccttacgcag
      721 caatgaaatc tgcagtatct gcagcagata cagcaaaatc caaaggaata aatgcacttc
      781 atattagagt gcgcgcagtt ggaggagtag gacctagaat acccggtcca ggagctcaag
      841 ctgcaataag agcattagct agagcaggtt ttagaatcgg aagaatcgat gatgttactc
      901 cagttcccca cgatacaacc agaaagccag gtggccgaag gggaagaaga gtataacatt
      961 acaatacttt ttaatttttc ttttaagtag aaaacaccca agctttaaaa attatgaacg
     1021 taatattgag agagattcag ttacaataat aaattaatcg atattactat catttttgaa
     1081 tgatataaaa ataaattaaa aaaataagta tttgatgaaa tgcatttatt tattgacatt
     1141 atcttttatt ttattcatcg tcaatctctt tattctgctt aagtttctct gcaaatgaca
     1201 tatattttcc atgttcttca attttgatag tagtatttat tttaacatta atccctattg
     1261 ttcgaaataa cgatagtaat tctcctccag aaattttttg gtttatttct ccactattaa
     1321 taagttctac tattctattt attattgctc tagtttctga tggaaattga gattctgcta
     1381 attttaatac ttcatctcct ctatcataca aatatgatcg taagatttcc ctttcggaag
     1441 gctcacgttt tttaatatct ttttctttat tttctttttt ctgttttatc atacgttgca
     1501 ttatttcttg cattttctta gctttaagaa tttcaatatc tagatcttct tccatttaac
     1561 ctttaatcac tcctatcgga actaactttg ctactttagt tgcaatcccc aatttatctg
     1621 atacattcac tactgagtct acatctttat aagctaaagg tgtttcctct actattccat
     1681 tttttgttaa tgcttttata tatattccct ttgaatcaag ttcagctttt actttttcaa
     1741 tggtataact tcttttagca gcacctcttg acatagttct tcccgctcca tgtgcagttg
     1801 atccaaaact taaatcaaga gattttttat ttccgactaa tatccaactt gctgttccca
     1861 ttgaccctgg tataattaca ggttgaccta tttgtcgata tttctctggg acctgttcca
     1921 tacctgccgg aaaagcccta gtagcacctt ttctatgaat tactaagtct ctcattcctt
     1981 ctccatcaac cttatgacgt tctactttgg ctatattatg tgctacatca taaaccaatt
     2041 tcatatctaa tcctgtttct gaactcgaaa atattttttc aaatgttttt ctagtaaaat
     2101 gggtaattat ttgcctattt gaccatgcaa aattcaaagc tgaatacatc gcttttctat
     2161 aattttctcc ttcaattgaa ttatttggaa cacatgcgag ttctctatca attaattcaa
     2221 tattatattt tttcaaagat ttttctgaat aacgtaaata atcactacac acttgatgac
     2281 caaatcctcg tgatccgcaa tgtattagta ctgttatttg acctttttct aaccccatca
     2341 tatttgctgc attttcatca aaaatttctt ggatttgttg aatttccaga aaatgatttc
     2401 ctgagcctaa gcttcctaac tgaggagctc ctctttttct ggcagtatca gaaacacttt
     2461 ctggattagc tcctatcatt ctacctttct cttcgcagac ttcagaatct tgtttatatc
     2521 cataaccatg tttaatagcc cagtcaactc cttcgatcaa taattcatct aattctgatc
     2581 ttgtgagttt aatagatccc tcgctaccta cccctgatgg tattgaatta aataattcat
     2641 ttactaaatc ttttaaatga ggtttaactt gatttacgtg aagatttgat ctgagtagtc
     2701 ttactccaca gttaatatca tatcctacac ctccaggact aataactccc tcttccatgt
     2761 cagtagctgc tactcctcca accggaaaac catatccttc atgaccatct ggcaatacca
     2821 ctacatgttt tcttacacca gggagtgttg aaacattgac agcttgatct aatgttctat
     2881 cttcactcat ttttaaaatc aagctatcat ctgcataaat cgtaactggt acattcattt
     2941 ttttagaatt atcaacatta attttaaatt caaattcgtt taatttctca ataacatgat
     3001 ttccacgctt ggttattatt ttattcattg tatttggtta tttttattat tattataagc
     3061 cttgcttgac tattaaagat attttattaa agctttaata tatgattcaa atcttttaaa
     3121 cgactttcta tatgccctaa tactattact tcatcctttt ctatttcttc atcgattttt
     3181 atatattaat ttattatgat aactacagac atacacatac tctttattat ttctgttaat
     3241 gtttgtaaaa ttaaattttt tttcttctaa tttttccatc tttattctaa taatcatttt
     3301 ggctagagat cttcaatttt tgatttcttg tttactgtta ttccaagatc agaaaaataa
     3361 tcaccattct aatctactat ttttttcaaa gatcctttgc attttattaa aataatctta
     3421 tcttctctaa tcttgaaatt gttaaataat acaataaatg tcttaggtat gagttaattt
     3481 tattatttta tgttttccag ctttcgacat atatcaatct ctcaattcaa ttaaacctca
     3541 ttcttcatta ttatatatta tttgtaacta ctctcatcgg atattaatta ctctgagcat
     3601 ttcaatactc accatttttt gtaataataa atttaaactc tcgttttaaa gagatttgat
     3661 ttccactctc ggggggaggg cgctctaaag agacagtata tgtataaaat atccttttct
     3721 aaattttttt atttttatta tatgaatgta attactagtt gtgtatttat attatcttca
     3781 ataacaataa ataaatgaat ttctataatg ttcactggta taatagaatc tctgtgtgaa
     3841 ataaaatcta ttaacgagat aaaaaaaggc aattcgaaat atcctgctga aattaaattt
     3901 tctatagata ttgggaattt aagtaagggt ttaaagattg gtcaaagtat agcagtaaat
     3961 ggagtttgtt taaccataac caaattaaat aataatattg ccgaattttc agtaattgga
     4021 gaaacagtaa gaaaaacatg tctagattca ataaagaaag gagagaaggt taatattgaa
     4081 agaagtatga gagtatccga caggtttgat ggtcattttg ttcaaggtca tgtagattgt
     4141 acaggtatta tctacgacaa aatagttttc cccacagaaa caaaacttat tatcaaaatc
     4201 tttggggaac taaaagaatc tttccgatat gtagtacgaa agggttcaat tacaattgat
     4261 ggaattagct taaccgttgt agatattgac aataaaaact tagtatccat atctttgatt
     4321 cctcatacat taaaaaaaac tactttaggg ataaaatcaa ttggagataa agttaatatc
     4381 gaatttgata ttattgggaa atatatttca agacttttac cataaaattg gtaataacaa
     4441 cttttgtagt aaaatatata atgactgctg tttttataat ttgtgtttgt caactgtaaa
     4501 agatgctata gatgcactca gtcaaggaaa atttgttctt attcatgatg atgacagtag
     4561 agaaaatgaa actgatatga taattgctgc agaaaatata acaccatatc atatagcaac
     4621 tataagaaat gatgcagggg gcctattgtg cctagctata gatagttata tttctacgaa
     4681 gttgggatta tcatacatac atgatatttt aaactgtgtt tctaatgatc atcctttatt
     4741 taccgctatg acaaatggtc gtgctccata tggagataaa ccttcattct caatttctgt
     4801 taatcataga gatacttaca caggaattac tgattatgat cgtgcgttaa caatttccaa
     4861 acttgcatca gtttgcaaaa atattgataa tggtggagta tcagaattta tcaagtgttt
     4921 tagaactcca ggtcatgttt ccctattgat tgctgcaaaa gggctattaa aagaacgatt
     4981 tggtcataca gaacttgctg tttatttggc aaagattgca ggtctaaccc cagctgttgc
     5041 aatatgtgag atgcttgatt ctcatacttt tagatctctt tctattgatg aagcatataa
     5101 atatgcaaaa ttaaaagaca ttcctttatt agaagctgat caattaaaga catattcggt
     5161 gatttaaatt tcaattgatc aaagttcact tagcaatagt tgtttctgaa tttaatcatg
     5221 aaattactga tgtaatgtta gaagaagcaa gagttcatgc atcaaagtta aactcgaatg
     5281 t
//
LOCUS       DATAFW010000051         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4776544, whole genome shotgun sequence.
ACCESSION   DATAFW010000051 DATAFW010000000
VERSION     DATAFW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4776544"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VHJ57_01200"
     CDS             <1..228
                     /locus_tag="VHJ57_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487182.1"
                     /translation="LSNKKINAVAIDSPSIDSAIEPEFPVHKILLKNDIIIIENICNL
                     AQIDKTKFKLIAIPLKLLGASGSPVRALAII"
     gene            373..1011
                     /locus_tag="VHJ57_01205"
     CDS             373..1011
                     /locus_tag="VHJ57_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX2487183.1"
                     /translation="MPTNWLTWARGNDKLILGALEKQGSILVEATSLLIELLSDYEKS
                     LSENKDKIKDLEVKGDSITHELFTTLSQTFVTPLDREDLSGLTSTIDQVLDYVDGTAD
                     RFVLFKIKKPMVYMLDLAKILHLCSQEIHYVLKKLQSTKKNGNFIEHCNKIKKYEREA
                     DHLYRKAIAELFETNDAVQILKNYEIYQNLESSIDRCMDVADIVEDIVLKYG"
     gene            960..2039
                     /locus_tag="VHJ57_01210"
     CDS             960..2039
                     /locus_tag="VHJ57_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX2487184.1"
                     /translation="MYGCGRYSGRYSSQIWIIVLCRNDVKVEPIALIITGIIAALFFD
                     FVNGFHDAANAIATVVGTRVLKPIQAVGMAALANFIGPFVFGTAIAATVGKGIIQPNF
                     STAEVILAGLVGAIVWDLLTWYFGLPSSSSHALIGGLIGSALIFGGFEAIVFGGVQKV
                     LTFLVVSPILGFVIALTFGILIIYFLKKYQPGKINRVFGKLQIGSSAFFSLTHGANDG
                     QKTMGVITALLISGGLLNAENFEVPIWVIFSASIAMAFGTFLGGWRIVKTMAFRITEL
                     KPYQGFCAETGGGVILTAMAWLGIPVSTTHAITGAIMGVGTTRRLSAVRWGIGKKIIY
                     AWIITIPASAAIGALVLLLLKLLGL"
     gene            complement(2379..2627)
                     /locus_tag="VHJ57_01215"
     CDS             complement(2379..2627)
                     /locus_tag="VHJ57_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487185.1"
                     /translation="MILNWIRRKTWKPKGKIYSLKCNQCGDGFISNHSMEAPICGSCL
                     QKVKSIDLTVDTEKKCIICGRNGAVYSSNKCNDCYFKK"
     gene            complement(2939..3466)
                     /locus_tag="VHJ57_01220"
     CDS             complement(2939..3466)
                     /locus_tag="VHJ57_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487186.1"
                     /translation="MKIKVVISLFVLVLIVTNNTFFVYGQNSNNPNVFENIFNNLKNK
                     IIDLTKSVEYTIDIKADQIFLNDTIKNSVLNKNEPTEYLIPILEYNLLGFDIFATDIK
                     VRTESSTIDEEKKNKLRIEFPIMQAKNVNVKNEIFDLNYEDIDLSSTYTIYDSELDKF
                     TIHIPVYVAARYLPK"
     gene            3600..>5069
                     /locus_tag="VHJ57_01225"
     CDS             3600..>5069
                     /locus_tag="VHJ57_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2487187.1"
                     /translation="MNEGVPSNPIDKNTKWNKIPQIAWFTLFILGSVDLISTAGETMI
                     IPAIPDIINEFNITYSDSSWILSSYLIAGAVMTPVASKLSIIFGKKEILLTLMILYVI
                     GTLLAGFAMNFSFLIVARVIQGLGLAMFPLAFSIIQTVFPKESLAIGQGIIIALFSSG
                     SVIGLVVGGYVIDNFGWQIIFFVFVPISILTIILINKKINIPNEEILIKENTESITTS
                     TNKKLSKLNLIDFKGTITLAVLISSFLIVLSNLKEFDPQQFQLTIIFTIITLISLILF
                     IFFSKRNKNPVISLEIFKHKILLFTNIILMISGLTTLMIYQMLPILIRNPFPVGFEGD
                     AIKVAEIQLPFMIVLFLVSVFSGFLVSKLGNIKPTLLGSIISLLGFFSIFFFHFSEFV
                     IVVNLVIIGIGISFLQVGGFNIITASTPKKLTEISVGVTSLLFIIGMSIGPVISSLFL
                     QNFKTLIYNNNNFLPSSEAYNLIFLTAASISLVPAILILY"
BASE COUNT         1782 a          650 c          725 g         1912 t
ORIGIN      
        1 ctatctaata aaaaaattaa tgctgtagca attgatagtc ctagtataga ttctgcaata
       61 gaaccagaat ttcctgtcca taaaatactt ttaaaaaatg atataataat aatagaaaat
      121 atatgtaatt tagctcaaat agataaaaca aaatttaagt taatagcaat accactcaaa
      181 ttacttggag catcgggatc tcctgttaga gctctcgcta taatctaatg tttataataa
      241 aaataattac ataagtcata acacttatta ttattattat tattattatt ataatcatca
      301 gaatcatcag gtaaaaaata tctaatgctt tataaaatat taaattttat tgtgttaagg
      361 tgatatggag atttgcctac aaattggctt acttgggctc gtggtaatga taaattaatc
      421 cttggagctt tagaaaaaca aggatctatt ttagttgaag caacttcctt actgatagaa
      481 cttttatccg actatgaaaa atctctttct gaaaataaag ataagataaa agatcttgaa
      541 gttaaaggag atagtattac tcatgagtta tttacaactt tatcacagac atttgttact
      601 ccattagatc gagaagatct atcggggttg actagtacaa tagatcaagt tttagattat
      661 gtagatggta cggcggatag gtttgtacta tttaaaatta aaaaacctat ggtctatatg
      721 ttagatcttg caaagatttt acatttatgt tctcaagaaa tacattacgt gttaaaaaaa
      781 ttacagtcaa caaaaaaaaa tggaaacttc atagaacact gtaacaaaat taaaaaatat
      841 gaacgtgaag cggatcatct ttatagaaaa gctatagcag aattatttga aactaatgac
      901 gctgtacaaa tacttaagaa ttatgaaatt tatcaaaatc tagaatcttc catagataga
      961 tgtatggatg tggcagatat agtggaagat atagttctca aatatggata attgttcttt
     1021 gtaggaatga tgttaaagtg gaacccatag cgttaattat aacaggaata atagcagctc
     1081 ttttttttga tttcgtaaat ggttttcatg atgcagctaa tgcaatagca actgttgtag
     1141 gaactagagt tttaaaacca attcaagcag taggtatggc agctctagct aattttattg
     1201 gaccttttgt ttttggtact gctatcgcag ctactgtagg caagggaatt atacaaccta
     1261 atttttcaac tgcggaagtt attttagctg gactagtggg agcaatcgta tgggatttac
     1321 ttacttggta ttttggttta ccgtcgtcta gtagtcatgc acttattggt ggattaattg
     1381 ggtctgcatt aatttttggt ggttttgaag caatagtttt tggaggtgtt caaaaagtat
     1441 taacatttct tgtagtttct cctattcttg gatttgttat tgctttgaca tttggaatat
     1501 tgataatata ttttctgaaa aaatatcaac caggaaaaat aaatcgagta tttggtaaat
     1561 tacagatagg atcatctgcc tttttctctc ttacacatgg agcaaatgat ggacaaaaaa
     1621 ctatgggagt aatcactgct ttactaatat ctggtggttt gttaaatgca gaaaatttcg
     1681 aagttccaat ttgggtaatt ttttcagctt ctatagccat ggcttttgga acatttttgg
     1741 gcggatggcg aatagtaaaa acaatggctt ttcgtattac cgaattgaaa ccatatcaag
     1801 gtttttgtgc agaaactggc ggtggagtaa tattaactgc tatggcatgg ttgggaattc
     1861 cggttagtac aactcatgcc ataacagggg ctataatggg tgtaggtact acaagaaggc
     1921 tttctgcggt acggtgggga ataggaaaaa aaataattta tgcttggatt attacaatac
     1981 ctgctagtgc ggcaatcggt gcgttggttt tgttattatt aaagttatta ggactttaac
     2041 ggattttact ctccttatta tcattattat gtcaatgatc atactaatga atttacgaaa
     2101 aaatcttgat attgttgttt gtggtttatg tttaaatata tacatatatt tttaaataat
     2161 atgtagtttg ctacatctat aaatattata aaaggaaatt acatataaaa agtagagtag
     2221 atatatatca atatacggta acaaaaaaat aatactaatg ttataatata tattaaatct
     2281 aataaaaaaa taaaaaagaa aatattataa attagagata ttaattgaat tatctcaata
     2341 atatataaaa aaatctttaa taagatatag ttaataactt attttttgaa ataacaatca
     2401 ttacatttat tagaagaata aacagctcca tttcttccac atataataca tttcttttcc
     2461 gtatcaactg ttaagtcaat cgatttaact ttctgtaagc aactgccaca gattggtgct
     2521 tccattgaat gattagatat aaatccgtcc ccacattggt tacatttcaa actatagatt
     2581 tttccttttg gcttccatgt ctttcttctg atccaattta aaatcaaatt ctttacatac
     2641 tttatttaga attttaagat aattaaattt ttgcctctaa aataatttaa ataatatatt
     2701 aaaagcttat aatatttata aaaaaaagtt atattaaaaa agaaatatga tttaaacaaa
     2761 tttaattata tatttatgcc atgttcatat tagaattttc aaaattattg attaattatt
     2821 aattaattat tgataatgac tattttagaa ataatcaaat agtgctaatt atgtcatatg
     2881 agtcaaattt aaaagaatat cctatgataa ttctcgaact tgattataat gtttatgttt
     2941 attttggtaa atatctagca gctacatata ctggaatatg aatagtaaat ttatctaatt
     3001 ctgaatcata aattgtataa gtagagctta aatcaatatc ctcataattt agatcaaaaa
     3061 tttcattctt tacattcaca ttctttgctt gcataatagg aaactctatt ctcaatttat
     3121 tttttttctc ttcatcgatc gtacttgatt cggttcttac ttttatatct gtagcaaata
     3181 tatcaaaacc taataaatta tattctaaaa tgggaattaa atattctgtt ggctcatttt
     3241 tatttaatac agaattcttg atagtatcat ttagaaagat ttgatcggcc ttaatatcta
     3301 tggtatattc cacacttttg gttaaatcga taattttatt ttttaaatta ttaaatatgt
     3361 tttcaaaaac gttaggatta ttagagtttt gaccatatac aaaaaatgta ttatttgtaa
     3421 ctattaatac taaaacaaat aaagaaatta caaccttaat tttcatattg ataaatatta
     3481 atttaaagat ttattatctc atatattcct tgattttggt ttaactaaat aaaatcttgt
     3541 atttgcacct attttaatat cctttttatt tatataagga aataattcca taaaattaag
     3601 tgaatgaagg cgttccatct aatcccatag ataaaaatac taaatggaat aaaattccac
     3661 aaatagcatg gtttacgctt tttattcttg gttctgtgga cttgatttct acagctggtg
     3721 aaacaatgat aatacctgca attcctgata ttattaatga attcaatatt acatattctg
     3781 attcatcttg gatattgagt tcatatttaa tcgccggagc agtaatgaca cctgttgcat
     3841 caaaattgtc tatcattttt ggaaaaaaag aaattctttt gactttgatg atattatatg
     3901 taattgggac attactagca ggattcgcta tgaatttttc ctttttgatt gttgcaagag
     3961 taattcaagg cttaggactt gcaatgttcc ccttagcctt cagtatcata caaactgttt
     4021 ttccaaaaga atcgcttgcc ataggtcaag gaataatcat tgctcttttt tcctcagggt
     4081 ctgtaattgg tctagtagtt ggaggatatg tgatagataa ttttggatgg cagattatat
     4141 tttttgtatt tgttcccatt tctattttaa ctataattct cattaataaa aaaataaata
     4201 ttccaaatga agaaatctta atcaaagaaa atactgagag tattactaca tcaactaata
     4261 aaaaattgag taaattaaat ctaattgatt ttaaaggaac aataaccttg gcagtcttga
     4321 tatcatcttt tttaatagta ttatcaaatt taaaagaatt cgatcctcaa caatttcagc
     4381 tcacaattat tttcactata attacactaa ttagtcttat attgtttata tttttttcaa
     4441 aaagaaataa aaatccggtc atttctcttg aaatctttaa acataaaatt cttcttttta
     4501 caaatatcat tttaatgatc tcaggtttaa caacccttat gatttatcaa atgttaccaa
     4561 tattaatacg taatcctttt ccggtaggtt ttgaaggcga tgctattaag gtagctgaga
     4621 ttcaacttcc atttatgatc gtactatttt tagtttctgt tttttctgga tttctagttt
     4681 ccaaattagg aaacattaaa ccaactttat taggaagcat aataagttta ttaggatttt
     4741 ttagtatatt cttctttcat ttttcagaat ttgttattgt agttaattta gttattattg
     4801 gaataggaat atctttttta caagtaggtg gatttaatat aataacagca tcaactccaa
     4861 agaaattaac agaaatctca gtaggagtga cctcattatt gtttattatt ggaatgtcaa
     4921 taggaccagt tatctcgtct ctatttttac agaattttaa aactttgata tataataata
     4981 ataatttttt gccttcttca gaagcctata atttaatttt tttaactgca gcaagtattt
     5041 cattagttcc agctatttta attttatat
//
LOCUS       DATAFW010000052         3806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_373528, whole genome shotgun sequence.
ACCESSION   DATAFW010000052 DATAFW010000000
VERSION     DATAFW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373528"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..735
                     /locus_tag="VHJ57_01230"
     CDS             172..735
                     /locus_tag="VHJ57_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487188.1"
                     /translation="MIRIGITGNPGVGKHTITDLLSKKIKNSKIIDINKIIISNQAFK
                     NGTFEVDLKKTRLFLLKELVNDNVIVVGHLLPYLIKRNELDFIAILRKSPYSLIDVYE
                     KRKYSNKKLQENILCEILGICFYDTLKVFGKLKISEIDTTCNDPEESVKKIIYNYDDK
                     SKRQIGLVDWLDIIYKNGDNRKFLYIN"
     gene            821..1231
                     /locus_tag="VHJ57_01235"
     CDS             821..1231
                     /locus_tag="VHJ57_01235"
                     /inference="COORDINATES: protein motif:HMM:NF026244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsm family RNA-binding protein"
                     /protein_id="HEX2487189.1"
                     /translation="MSVAFRKFGEESIQFLGKKVSIETSDKKTYDGTLIGINERLDII
                     LEDLETSGIIKIIINGSFIKEIRLMEKPFDLKALAERFSRVFPGLVRVREDVGAIIVM
                     DKIKVTQNGIEEGTGLAADRIKAIYDEFIRETKK"
     gene            1234..2826
                     /gene="tgtA"
                     /locus_tag="VHJ57_01240"
     CDS             1234..2826
                     /gene="tgtA"
                     /locus_tag="VHJ57_01240"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HEX2487190.1"
                     /translation="MSFEVRHSDLGGRIGKLNTAHGIIDTPAFIPVVHPINQLVDVKL
                     LKKMGFRTIITNAYITLKHYGDHARKKGIHRIIDFDGIVMTDSGGYQVLEYGNIDIGP
                     REMAKFELDIKTDIAVPLDKPTGFGLEYSKAINYVNQTISNVKETVEVIKKNNNNNCN
                     TNEINTIWAAPVQGAEHLDLVEQSAKLFDDMNFKLMALGSPVELMETYEFAKLSEIIC
                     TLKKTIPSKPIHLFGAGHPLTIPLAVALGCDMFDSASYILYAKDNRYMYSNGTTRLEE
                     ITYFPCSCSICNSYTPHELLEATEIERIIQLSLHNLYVLKKEVNSVKQSILDGRLWEY
                     IMSKAHNHPKLMDAVRLFKNFDYLEDGTPISKDKAIYFNEPYDQYRPEAKRFRRMVTK
                     YIINNKNKLILYPETKIHPFYSSKNFVKIKELFSDGEVFSYNPFLGVIPAEISDIFPA
                     SHNIISVENDSQSFAANYSTFIDAMKFFLKANQGKSIIIIADEFMKQIIYYFYTQENI
                     IEIFNLDIKIVDYKENIIFEIS"
     gene            complement(2852..3469)
                     /gene="leuD"
                     /locus_tag="VHJ57_01245"
     CDS             complement(2852..3469)
                     /gene="leuD"
                     /locus_tag="VHJ57_01245"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX2487191.1"
                     /translation="MEPFQNFESKATPFPYVNVDTDQIIPKQFLKLTQKTGFGKYLFY
                     DWRYNKNGEPKKDFVLNNPRYSEYPILLTLDNFGSGSSREHAVWAIMDYGFRVIISPS
                     FADIFYSNCIKNGILPIILPKKEIENLFDNPDYIIKVDLNTQQIELKPISRQLKFQID
                     PSKKRMLLEGLDEIGMTFQLESHISDYENKVVHSDFSINVLPKKE"
     gene            complement(3500..>3806)
                     /locus_tag="VHJ57_01250"
     CDS             complement(3500..>3806)
                     /locus_tag="VHJ57_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX2487192.1"
                     /translation="KVSPKVHAMVVPGSQSVKLKAEKLGLDKIFIESGFEWRESGCSM
                     CLGMNPDILSSGERCASTSNRNFEGRQGVGGRTHLVSPVMAAAAAIEGHFVDVREWI"
BASE COUNT         1446 a          503 c          579 g         1278 t
ORIGIN      
        1 cattacacaa aaacggtgta tttgacaagg gctgggaaga aggttaaaaa acacaataca
       61 ctacaattaa atagaaaatt tcaactcatt ttttatattt ttgttgtagg cttttttcta
      121 tttgtctaga tttagattta ggttgttaga ttttatattt tactatatag tatgatacga
      181 ataggaatta caggtaatcc tggtgttggt aaacatacaa taactgattt gctttctaaa
      241 aaaataaaaa atagtaaaat aattgatata aataaaataa ttatatcaaa tcaagctttc
      301 aagaatggaa cattcgaagt tgatttaaaa aaaactagat tattcttatt aaaagaacta
      361 gttaatgata atgtaatagt agttggacat cttttaccat atttaataaa aagaaatgaa
      421 ttagatttta tagccatact aaggaaatct ccatattctt tgatagacgt atatgagaaa
      481 agaaaatatt ccaataaaaa attacaagaa aatatactct gtgagatttt aggtatatgt
      541 ttttatgata ctttaaaggt atttggtaaa ctgaaaattt cagaaataga tacaacttgc
      601 aatgatccgg aagaatcagt gaagaaaatt atttataatt atgatgacaa gtcaaagaga
      661 caaataggtc tagttgattg gttagatata atatataaaa atggagataa cagaaaattt
      721 ttatacatta actaaatata aaaatgctaa attaggataa caaaaaaaga tagatattga
      781 aattatatat agcttctatt ataaccacaa tagaattagc ttgtctgtag catttagaaa
      841 atttggagag gaatcaattc agtttttagg aaagaaggta tctatagaaa catcagataa
      901 aaaaacatat gatgggacat taattggaat aaatgaaaga ttagatataa tattagaaga
      961 tttagaaact agtggaataa taaaaataat cataaatggt tcatttataa aggaaattcg
     1021 attaatggaa aagccatttg atttaaaagc tcttgcagaa cgattttcta gggtttttcc
     1081 aggtttagta agagttagag aagatgtggg agctattata gttatggata aaatcaaagt
     1141 tacacagaat ggcattgaag aaggtacagg acttgcagcc gatagaataa aagcaattta
     1201 tgatgaattt atacgggaga caaagaagta acattgtcgt tcgaggtcag acatagtgac
     1261 ctaggcggac gtataggaaa attaaataca gcacatggaa tcatagatac gccagcattt
     1321 attcccgttg ttcatcccat aaatcaacta gtagatgtaa aattgctcaa aaaaatggga
     1381 tttagaacca ttattactaa tgcatatatc actctaaaac actatggaga tcatgctcgg
     1441 aaaaaaggaa ttcatagaat aattgatttc gatggtattg tgatgacaga ttctggaggg
     1501 tatcaagtgc tagaatatgg aaatattgat ataggaccta gggaaatggc aaaatttgaa
     1561 ttagacataa aaactgacat agcggttcct ttagataaac caacaggttt cggacttgaa
     1621 tatagtaaag caattaatta tgttaatcaa acaattagta atgttaaaga aactgttgaa
     1681 gtaattaaaa aaaataataa taataattgt aataccaatg aaataaatac gatatgggca
     1741 gctcctgttc aaggtgcaga acacttagat ttagtagaac agtctgcaaa actctttgat
     1801 gatatgaatt ttaaattaat ggctttagga agcccagttg agttgatgga aacctatgaa
     1861 tttgctaaac tttctgaaat aatttgtact ttaaaaaaaa cgattccgtc taagcctatt
     1921 catctttttg gagcggggca tccattaacc attccattag cagttgcatt aggatgtgat
     1981 atgtttgatt ctgcatcata tattttatat gctaaagata atagatatat gtattcaaat
     2041 ggaactacaa gattagaaga aattacatat ttcccttgct cttgctctat ttgtaattct
     2101 tatactccac atgagttatt agaagccact gaaattgaac gaattattca attatcctta
     2161 cataatttat atgtcttaaa aaaagaagta aattcggtaa agcaatcaat actggatgga
     2221 agactctggg agtatataat gagcaaagct cacaatcatc caaaattaat ggatgctgtt
     2281 agattattta aaaattttga ttatctggaa gatggtacac caatctctaa agataaagca
     2341 atttatttta atgaaccata tgatcaatat agaccagaag caaaaagatt taggagaatg
     2401 gtaacaaaat atattattaa caataaaaat aaactaattt tatatcctga aacaaaaata
     2461 catccatttt attcctcgaa aaattttgtc aaaatcaaag aactattctc tgatggagaa
     2521 gttttttcat ataacccatt tttaggagtt attcctgctg agatatcaga tatatttcca
     2581 gcttcacaca atatcatatc ggtagagaat gattctcaaa gttttgcagc aaattattcg
     2641 acattcattg atgcaatgaa gtttttttta aaagctaatc agggtaaaag tataataatt
     2701 atagcagatg aatttatgaa acaaattatt tattattttt atactcaaga aaatattatt
     2761 gaaattttta atttagatat taaaatagta gattacaaag aaaatatcat atttgaaata
     2821 tcctagatac aataagatct tttaaataaa tttactcttt ctttggtaga acatttatac
     2881 taaaatctga atgtactact ttattttcat aatctgaaat atgtgattct agttgaaatg
     2941 tcattccaat ttcatccaaa ccttctaata acatcctttt cttagatggg tctatttgaa
     3001 attttaattg tcttgaaatt ggttttaatt ctatctgttg tgtatttaaa tctactttaa
     3061 taatataatc aggattatca aaaagatttt ctatctcttt ttttggcaat attattggta
     3121 atataccatt ctttatacag ttactataga aaatatcagc aaatgaagga gaaataataa
     3181 ctctaaaacc ataatccatt attgcccata ctgcatgttc tctagaactt ccacttccaa
     3241 aattatctaa tgtaagaaga ataggatact ctgaatatcg ggggttgttt aatacgaagt
     3301 cctttttagg ttcaccattc ttattgtaac gccaatcata aaataagtat tttccaaatc
     3361 cggttttttg tgttaattta agaaattgtt ttgggattat ctgatctgta tcaacattaa
     3421 catatggaaa aggtgttgct ttactttcaa aattttgaaa tggttccata ataattgatt
     3481 tagaggatat attgaatatt tatatccatt ctcgaacatc aacaaaatgt ccttctatcg
     3541 ctgctgctgc tgccataact ggacttacca aatgagttct acctccaact ccttgccttc
     3601 cttcaaagtt tcgatttgat gtactagcac acctttcacc cgaggaaaga atatccggat
     3661 tcatccccaa acacatactg catccagatt ctctccattc aaatccactc tctataaaga
     3721 ttttatctaa tcctaatttt tctgctttta acttgaccga ttgagatcct ggtacaacca
     3781 tagcatgaac ttttggtgaa actttt
//
LOCUS       DATAFW010000053         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_549548, whole genome shotgun sequence.
ACCESSION   DATAFW010000053 DATAFW010000000
VERSION     DATAFW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549548"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VHJ57_01255"
     CDS             <1..957
                     /locus_tag="VHJ57_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX2487193.1"
                     /translation="VTSVLRTNLPIKSYIEKKIDRNDLESTGRYIYNFKVNGEDKTYF
                     DPDYCIIHLDQKLAPGGYAWVFPKGDDKVNIGLGVQQLAFNLYNQTHGTTKNLKNLID
                     EYVKLNPVISEPEIPNTEEDQNNTWGTWQVSVRRQNDCLVGNGYMLVGDSAWMPKPLD
                     AGGIGPAIIAATIAGKNAVESIQASDVSESGLWKYNTDFIKEYGYKTAGLEVFRRMLQ
                     SLSNEQIDYGMKNFLSKFDIEKISQGEHPDFSTVDKLGMLIRGALNKNLAGDLKYTSQ
                     MNKKLVEHYNNYPQNPEGFTNWQYQLDGYLKEAYSYFKETKM"
     gene            complement(1159..1947)
                     /locus_tag="VHJ57_01260"
     CDS             complement(1159..1947)
                     /locus_tag="VHJ57_01260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2487194.1"
                     /translation="MPLINIWLKICYTILSKNKYSYLIYLDKLNLHVLGLGASYWKQV
                     ISTLRDIIPVYDKVNRVISLGKDDLYRYEGISGNIKSGDLVLDAGSGFGNMSLTTFKI
                     TVDKVRIVMYDPIYEMLQSSRLRLVVELKSSGIFEYMPFKDNTFDVVMCGYSLRDAIN
                     LESAISEIHRILKKNGRFLIVDLGKPDNKIIRLGVSIYLKYIIKIFAYLIARQTGLKF
                     QTLYGTFVKWPENHTLNSLLKKQFSQVYFSTKLMGGSIIVSSIK"
     gene            1963..>2478
                     /locus_tag="VHJ57_01265"
     CDS             1963..>2478
                     /locus_tag="VHJ57_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2487195.1"
                     /translation="MAREFICKECGSLLTHSNELTLESMKEIHSQLCIIKRQKLIAAL
                     SIQEIAAKSAPMIQSTVQVTHGGSNVPSDMTPPTSKPVTGETTLSLTGSGTDYSKVEG
                     PIDTSFKSKRQKVGKFKGINVWGPVDPPGQLGIWGDSVCVDFDICIGDGACIEACPVN
                     VYEWLETPGHPG"
BASE COUNT          872 a          386 c          403 g          817 t
ORIGIN      
        1 gtaacatctg ttcttcggac aaatttacca atcaaatcat atattgaaaa aaagatcgat
       61 agaaatgatt tagaatcaac tggtcgttac atatataatt tcaaggtaaa tggtgaagat
      121 aaaacctact ttgatccaga ctattgtata attcatttag atcaaaaatt agccccagga
      181 ggttatgcat gggtttttcc taaaggggat gataaggtca atataggatt aggagtacaa
      241 caattagctt ttaatttgta taatcaaact catggtacta ctaagaattt aaaaaatctc
      301 atcgatgaat atgttaaatt aaatccagtc atttctgaac cagaaattcc taataccgaa
      361 gaggatcaaa ataatacttg gggaacatgg caagtttcgg taagaagaca aaacgattgt
      421 ttggtgggga atggctatat gttagtagga gactcagcat ggatgccaaa gcctttagat
      481 gcaggcggaa ttggaccagc cataattgct gcaactatcg ctggaaaaaa tgctgtagaa
      541 tcaattcaag cgtcagatgt atcagagagc ggtctttgga aatacaatac tgattttatt
      601 aaagaatacg gatataaaac tgctggatta gaagttttca gaagaatgtt acaaagttta
      661 tcaaatgagc aaatagatta tggaatgaaa aattttcttt caaaatttga tattgaaaaa
      721 atcagtcaag gtgaacatcc tgattttagt acagtagata agctaggaat gttaataaga
      781 ggtgctttaa ataaaaatct ggcaggagat ttaaaatata catctcaaat gaataaaaag
      841 ttagtagaac actataacaa ctatcctcaa aatccagaag gctttaccaa ttggcaatat
      901 caattagatg gatatctaaa agaagcctac tcatatttca aagaaacaaa aatgtaacgt
      961 gataatgagt tgatgtagta atgggagtct ttgagccttc tatattcgta tcaatcttcc
     1021 cttatctgtt tatatcttta tttccaatta tcggtattat aattgtgaca agatccctat
     1081 taaaaattaa gtaatttatg ctattatata tgataatatg tcttataaat tataaagctt
     1141 aacaaattca tatcaaattt attttattga cgatacaata atagatccac ccattaattt
     1201 ggtactaaaa taaacttgtg aaaattgctt ttttaacaat gaatttaatg tgtgattttc
     1261 tggccacttg acaaaagttc catatagtgt ctgaaattta agtcctgttt gtcttgctat
     1321 tagatatgca aaaattttaa taatatattt taaatatata gaaactccta atcgtattat
     1381 cttattatct ggttttccta aatcgactat tagaaatctc ccattttttt ttaatattcg
     1441 atgaatttct gaaatagcac tttctaaatt aattgcgtct cttaaagaat aaccacacat
     1501 tacaacatcg aatgtattat ctttaaaagg catatattca aaaataccag atgatttgag
     1561 ttctacaact aatcgaagtc tagaactttg taacatttca tatatcggat cgtacataac
     1621 tatccttacc ttatctacag tgattttaaa tgttgtcaga gacatattac caaaaccaga
     1681 gcctgcgtcc aaaacaagat ctcctgattt tatatttccg cttatccctt cataacgata
     1741 taagtcatct tttccaagcg atattactct attaacttta tcatatactg gtataatatc
     1801 ccgtaaggtt gagataactt gtttccaata tgaagctcct aatcctagca catgtaaatt
     1861 taatttgtct agatatataa gataagaata tttatttttg cttaaaatag tgtagcatat
     1921 cttcaaccaa atattgatta atggcatgct ctaaaataga ttatggcccg tgaatttatc
     1981 tgcaaagaat gtggctctct tttaactcat tccaatgaat taactttaga aagcatgaaa
     2041 gaaatacatt cacagctttg tattattaaa cgacagaaat tgatagctgc tctatctatt
     2101 caagaaattg cggctaagtc tgctcctatg atacaatcaa ccgttcaagt tacacatggt
     2161 ggctccaatg taccctcaga tatgacacct cctactagta agcctgttac cggtgaaaca
     2221 actctctctc taacaggaag cggaactgat tattccaaag ttgagggccc tattgatacc
     2281 tcttttaaat ctaagcgaca aaaagttggc aaatttaaag ggattaatgt ttggggtcct
     2341 gttgatcctc ctggacagtt aggaatatgg ggcgatagtg tctgtgtaga ttttgatatt
     2401 tgcataggtg atggtgcatg tatagaagct tgtccagtta atgtctatga atggttagaa
     2461 acacctggtc atccaggt
//
LOCUS       DATAFW010000054         6139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_726337, whole genome shotgun sequence.
ACCESSION   DATAFW010000054 DATAFW010000000
VERSION     DATAFW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726337"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VHJ57_01270"
     CDS             complement(<1..295)
                     /locus_tag="VHJ57_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487196.1"
                     /translation="MIQKHSISFVIIGFFLTISFLGSAFVVDSFGVMDQNLFQSTTGG
                     ALDVNITSSPEVISKNKEVKFKINFFEPGTDKIQVHVDYDFQVLKNGTEIFSAA"
     gene            complement(879..1313)
                     /locus_tag="VHJ57_01275"
     CDS             complement(879..1313)
                     /locus_tag="VHJ57_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEX2487197.1"
                     /translation="MNDIFSSNDEYIPVEISNIGYADHLGLEGFLILKSIEGKEFPIN
                     AFSGEVAKHILRFREGDKNTIPTIYNLVEEIAVMQDLLLMEVRIYMSGSVLRANLYFR
                     GRKDTLILRNYRASDAIALASYYDVPIKVRKNLFEDELQTTE"
     gene            1416..2231
                     /locus_tag="VHJ57_01280"
     CDS             1416..2231
                     /locus_tag="VHJ57_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487198.1"
                     /translation="MRIKENILLKKGLRIIIFSVSSLIFSYVFYSKFTNPNYNDIIMF
                     TPVLFNISIGLLIITFISSIAICFGLWNLYVSNEPAKKNLKYFISFPFKEKKYILIMI
                     LSGIFYGIIFSFLSQIFVFTDNGSNSFEDNKFNPSIKIIPCCNTIGYVPITYVYLTEY
                     FFIFLIPLNIVLVFIVSFLVGFNISINAFMIKRLKDKNKLRVIGGIGATCGLFIGCPT
                     CAGSIITAIFGFSAGTTTISILASFQTVFIVISIPALMIAPFILAKTFKNRII"
     gene            2409..2579
                     /locus_tag="VHJ57_01285"
     CDS             2409..2579
                     /locus_tag="VHJ57_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487199.1"
                     /translation="MEDESLEKLLKSFIDEKSLIKLLRILEDENTGKNALKIDNKFRK
                     IVQTDMQNYFYN"
     gene            2663..3850
                     /gene="mpgS"
                     /locus_tag="VHJ57_01290"
     CDS             2663..3850
                     /gene="mpgS"
                     /locus_tag="VHJ57_01290"
                     /EC_number="2.4.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050504 - mannosyl-3-phosphoglycerate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate synthase"
                     /protein_id="HEX2487200.1"
                     /translation="MKLDLPRFTERFGSISIHGVQRVFELDSGSDKGKKVSENITGVG
                     NEQISEIESKMAIVIPIKNERLKLLEGVLSGIPHDCMTIIVSNSPRQPVDRYKLEKES
                     LQQFNRFANKNALIIHQRDEGLAETLREVNYNELIGSDQLIRNGKAEGMVLGMLLAKM
                     TGKEYVGFIDGDNYVPGAVNEYVKIFACGIAMSDTPYKMIRVSWIYKPKISESGIYFS
                     KWGRVSEITNHYLNSLISYYTGFETEVMRTGNSGEHCMSLKLAELLNFSSAFSVETYE
                     IVNLLEQFGGILPTENQDAMDKGVEVMQVETRNPHFHEDKGEAHLKEMINGSLGCIYH
                     SRICHPKLQEKILEELKNKSTLDNNEVPPVLEKINPFKEVDIKTFDKKLHDKATSLIH
                     WNY"
     gene            complement(3916..>6139)
                     /locus_tag="VHJ57_01295"
     CDS             complement(3916..>6139)
                     /locus_tag="VHJ57_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HEX2487201.1"
                     /translation="YLIFFFYMPKFKSTQDIIRIIDTKDQIRNFGVIAHVDHGKTTMS
                     DSLLAASGIISPSVAGQALALDSMKLEQNRQMTIRGANVTLFYEHEGKEYVINMIDTP
                     GHIDFTGRVTRALRAIDGVVVVSDSVEGIMTQTETVTRQALEERVRPVLYINKIDRLV
                     KELRLDPPAMQKWLSNIIAEFNKLVDIYAEPALKEKWKVSIQANNVAFGSAKDRWGFN
                     FKVAQKKGFSFKDVYDAYTSDDPSRIKALSEKAPLHEAVLGMVVQHHPPPHIAQKYRI
                     PKIWPGDLNSDIGKSLVNCDEKGPAVMMVTTINVDPQAGRIATGRLFSGTIKDGDEVY
                     LIDAKRPGKVQSVNIYMGNTREVVSVIPAGNIPALLGLDYAVAGETISSVKNIVPFES
                     IKYVSEPVVTIAVETKHPKDLPKLVEALRKITVEDPNLIIKINEESGETLMAGMGVLH
                     LEIATSLLQEAGLSITTSQPLINYRETIRTKGGPIMSKSPNRHNKIFMRVESLKEDII
                     EMIKNGHIKEDMDKKELAKILREKGWNADEARNVSAIDPSGNMLIDETKGVQFIQESM
                     DSIKSGFDDVVHSGPLAHETLRGVKFVLNHFVPHEDPAHRGLAQLMPATRRSMLGSML
                     IADPVLLEPILGIEVKCPQEQIGTVAGILSAKRGKLLNVDQKAVIAIMQGEVPASETF
                     DLSEIMRGGTAGKALWSTYFKSWQAVPQSVIRTLIADVRKRKGLNPEPPSPDEFIDKE
                     "
BASE COUNT         2131 a          926 c          911 g         2171 t
ORIGIN      
        1 ttgctgctga aaatatttct gttccatttt ttaagacctg aaaatcataa tcaacatgaa
       61 cctgaatttt atcagtacca ggttcaaaaa aattgatctt aaattttact tctttatttt
      121 tactaataac ttcaggagaa gaagttatat ttacgtccaa agcgccaccg gtagtcgatt
      181 gaaacaagtt ttgatccata acaccaaaag aatctacaac aaaagcactc cctaaaaagg
      241 aaatagttaa aaaaaatcct attattacaa aagaaattga atgtttttgt atcatgataa
      301 gtctaagagt tactgaatat atgtatttat ttattaaact attgaaaaca aatacctatg
      361 taattactat attattatta ttattattgc ttctattttc aactgctaaa aagacaataa
      421 ttatttttaa ttaatacaat atttattaaa aattgaaatg aaaaataact atttataaat
      481 gtccatggta atggttatat taatcaaaaa taaaaaaagt aaattatcta cttaaatcaa
      541 tatgctaata agtaattcac aaaattatga ataaaaatat ggaaaagtct actctccttt
      601 gttataaatt aaaggtttac acctatttta attgattttt tggtagacgt ttatattttt
      661 ataattaagt aaaatgttta gaagagtata ataaaataaa atatcaaaat tatattttta
      721 tagatagaga aaaaaaatta tattataatg atctttgtct agttatagaa atattagtaa
      781 atattttgtc ttagtttctg tttccaattc tgctttttaa atttttctta attataaaat
      841 gaacaatata ataaaacaat cataagtatc ttgttgggtt attctgtagt ttgtaattca
      901 tcttcaaata aattttttct cacttttatg ggaacatcat aatatgatgc tagcgcaatg
      961 gcatcagagg ccctataatt tcgtagaata agcgtgtcct ttcttcccct aaagtataag
     1021 ttagcacgta gtactgatcc gctcatatat attctgactt ccattaataa tagatcttgc
     1081 attacagcta tttcttcaac tagattgtat atcgtaggaa tggtattctt gtctccttct
     1141 ctgaacctga gtatatgttt tgcaacctca cctgaaaatg cattaatagg aaattccttt
     1201 ccttctattg atttcaaaat taaaaaacct tccaatccca aatgatcggc gtaaccaata
     1261 ttactaattt ctacgggaat gtattcatca tttgatgaaa aaatatcatt catacctatt
     1321 aatattagga taaactacga gataaatttt tcatatattc atataataat aaaatttatt
     1381 tttggtggtt tagtttagta gagtaattca caactatgag gataaaagaa aatattttat
     1441 tgaagaaagg tttaagaata ataatattta gtgtttcatc attaatattt tcttatgttt
     1501 tttattcaaa atttactaac ccaaattata atgatataat aatgtttaca cctgtcttat
     1561 ttaatatatc aataggatta ctaataataa cgtttatttc atcaatagct atttgtttcg
     1621 gtttgtggaa tctttatgtt tcaaatgaac cagcaaaaaa aaatttaaaa tattttattt
     1681 ccttcccatt taaagaaaaa aaatacattc taataatgat attatctgga attttttatg
     1741 gtattatttt tagttttctt tctcagatat ttgtctttac tgataatgga tcaaattctt
     1801 ttgaagataa taaatttaat ccctcaataa aaataattcc atgttgtaac acaatagggt
     1861 atgtacctat aacatatgtc tatttaactg aatatttttt tatatttcta attccactta
     1921 atatagtatt agtatttatt gtttcattct tagtaggctt taatatttct ataaatgctt
     1981 ttatgataaa aagattaaaa gataaaaata aattaagagt aataggaggt attggtgcaa
     2041 cttgtgggtt atttattgga tgtcctactt gtgcaggtag tataataaca gcaatttttg
     2101 gatttagtgc tggaaccaca actatatcaa tattggcatc gtttcaaact gtatttatag
     2161 ttataagtat tcctgcatta atgattgcac catttatact tgctaagact tttaaaaatc
     2221 gaataattta aaaatatttg aaattgttaa ttaggtttta tattaataca aatttcattt
     2281 tataaattta ttcaaaattt ttgtttactg aaaaaaattg agaaatttta tttaaaaaaa
     2341 aaagggaaaa ttttaatatt atcagattga atatcagtta aataaaatag tatcacttct
     2401 tttacaaatt ggaggacgaa tcattagaga agttattaaa atcattcatt gatgaaaaat
     2461 cgttgattaa attacttaga atacttgagg atgaaaatac aggaaaaaat gctctaaaaa
     2521 ttgataacaa gtttagaaaa atagtacaaa cagacatgca aaattatttc tataattaaa
     2581 atttattatt atcatttatc tgatttaaat atatgtaggt ctcgttttac attggttttt
     2641 actaaaaaag gagaaataat atatgaaatt agatttacct cgatttactg aaagatttgg
     2701 atcaataagt attcatggtg tacagcgagt ttttgagctg gattctggtt cagataaagg
     2761 aaaaaaagta tcggaaaata ttacaggagt tggtaatgag caaatttcag agatagaaag
     2821 taagatggcc atagttattc ccataaaaaa cgaaagattg aagctattag agggtgtttt
     2881 aagtggaatt cctcatgatt gtatgactat tatagtttca aatagtcctc gccaaccagt
     2941 cgatagatac aaattagaaa aagagtcact tcaacaattt aatcgatttg ctaacaaaaa
     3001 tgcattaatt attcatcagc gtgatgaagg tcttgctgaa accttaagag aggtgaatta
     3061 caatgagtta ataggttctg atcaactcat tagaaacggc aaggcggaag gaatggtatt
     3121 aggaatgctt cttgctaaaa tgactggtaa agaatatgtc ggtttcattg atggagataa
     3181 ttatgtacct ggtgctgtca atgaatatgt aaaaattttt gcctgcggca tagcaatgtc
     3241 tgatacacct tataagatga taagagtttc ctggatttat aaacccaaga tttccgaaag
     3301 cggaatttat ttttcaaaat ggggtagagt atctgaaatt actaatcact atttaaattc
     3361 tcttatttca tattatactg gatttgaaac tgaagtaatg cgaactggaa attcaggtga
     3421 acattgtatg tctctcaaac ttgcagaatt acttaatttt tcttcagcat tttctgtaga
     3481 aacttatgaa attgtaaatt tacttgaaca atttggcgga atattaccta cagaaaatca
     3541 agatgcaatg gataaaggtg tagaggtaat gcaagttgaa accagaaatc cccactttca
     3601 tgaggataaa ggagaagcac atttgaaaga gatgattaat ggatcactag gatgtatata
     3661 tcatagtaga atatgtcatc caaagttaca agaaaagata cttgaagaat taaaaaataa
     3721 atcaacacta gataacaacg aagttccacc ggtgttagaa aaaataaatc cctttaaaga
     3781 ggtagatata aaaacttttg ataaaaaatt acatgataaa gcaaccagtt tgatacattg
     3841 gaattactaa agtgctgtca aattcgttta ataccaaaat ataaaaataa attagagaat
     3901 ctgtaagttg ctctatcatt ccttgtcaat aaattcatca ggacttggag gttctggatt
     3961 aagccctttt cttttgcgta cgtcagcaat aagggttctt ataacagact gagggactgc
     4021 ttgccaactt ttaaagtaag tactccagag tgctttacct gctgtcccgc cacgcataat
     4081 ctctgataaa tcaaaagttt cagaagctgg aacttcacct tgcataatag ctataacagc
     4141 tttttggtca acatttaata atttacctcg ttttgcggat aaaattcctg ctacagtacc
     4201 tatttgctct tgaggacatt ttacttcgat tcctagtata ggttccaaaa gtactggatc
     4261 tgcgattaac atagaaccca acatcgatct tcttgttgcc ggcatcaatt gtgctaatcc
     4321 tctatgagca gggtcctcgt gtgggacaaa atgattaaga acaaatttaa cacctctaag
     4381 ggtctcatgt gctaggggtc ctgaatgcac tacgtcatca aatcctgatt ttatcgagtc
     4441 cattgattct tgaataaatt gaactccttt agtttcgtcg atcaacatat ttcctgatgg
     4501 atcaatagca ctaacattcc tggcttcatc tgcattccag cccttttctc taaggatttt
     4561 agctaattct tttttatcca tatcttcctt tatgtgtcca tttttaatca tttctataat
     4621 atcttcttta agtgattcta ctctcatgaa tatcttatta tgtctattag gagatttact
     4681 cataataggt ccacctttgg tacgaatggt ctctctataa ttgattaaag gttgagaggt
     4741 agttatagat agacctgctt cttgtaacag tgaggtagcg atttcgagat gtaatacacc
     4801 cattccagcc attaatgttt ctccgctttc ttcatttatt ttgataataa gatttggatc
     4861 ttcaactgtt attttacgaa gagcttctac gagcttggga agatcttttg gatgtttagt
     4921 ttctacagca attgttacaa caggttcgga aacatattta atggattcaa aaggaacaat
     4981 atttttaacg ctagaaattg tttcaccagc aacggcataa tctaaaccta aaagagcagg
     5041 aatattgcca gcaggtataa cactaacaac ttctctagta tttcccatat agatgtttac
     5101 ggattgaact tttccaggcc gtttggcatc tattaaataa acctcatctc catctttaat
     5161 agtaccagaa aataatcttc ctgtagctat tctgcccgct tgtggatcca cattaattgt
     5221 agtcaccatc ataactgctg gacctttttc atcacaattt actaacgact tcccgatatc
     5281 agaatttaga tctcctggcc atatctttgg gattctatat ttttgagcta tatgaggcgg
     5341 aggatgatgc tgaacaacca tacctagaac tgcttcatga aggggtgctt tctcagaaag
     5401 agctttaatc ctggatggat catcagaagt atatgcatca taaacatcct tgaaactaaa
     5461 gccctttttt tgggctacct taaaattaaa tccccatcta tctttagcag atccaaatgc
     5521 tacattattt gcctgtatac tcaccttcca tttctctttt aatgctggtt ctgcataaat
     5581 atctactaat ttattgaact ctgcgataat atttgatagc catttttgca ttgcaggtgg
     5641 atctaaacgt aattccttta ccaagcgatc tattttattt atataaagaa caggtctaac
     5701 tctttcctct agagcttgtc tggttacagt ttcggtctga gtcataatac cttctacaga
     5761 gtcagaaaca actactaccc catcaattgc acgtaaagct cttgttactc taccagtaaa
     5821 atcaatgtgc ccaggtgtat cgatcatatt tataacatat tccttacctt cgtgctcata
     5881 aaatagagtt acattagctc ctctgatagt catctgccta ttttgttcaa gtttcattga
     5941 atcgagagct aacgcttgtc ctgcgacaga aggggaaata attccggatg cagccaaaag
     6001 actatcactc atggtagttt taccatgatc tacatgagca ataacaccaa aattacgtat
     6061 ttgatcttta gtatcaataa ttctaataat atcttgagtt gatttgaatt taggcatata
     6121 gaaaaaaaaa attaaatag
//
LOCUS       DATAFW010000055         5104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6187727, whole genome shotgun sequence.
ACCESSION   DATAFW010000055 DATAFW010000000
VERSION     DATAFW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6187727"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VHJ57_01300"
     CDS             <1..1194
                     /locus_tag="VHJ57_01300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2487202.1"
                     /translation="FPIQEKAIPIILEGNDVIGQAHTGTGKTAAYSLPILMKMKNARN
                     VQALVLVPTRELSIQVAREINKLAKYTETKIAAIYGGQNMRNQIIRLERGIDIIVATP
                     GRLIDHIGQKTVYLDTIDFVILDEADRMLDMGFIEDINYILSKVKHKKQLCLFSATIF
                     PEIIEISKKYMKNPIHITVNQNDITLKTIEQMYLITNEKEKYNHLCYLIKQSNNNQMV
                     IFTSTKQKAEELAMNLRKKELLKVTALHGDLSQRQRNYAMHKFRNEQEQILVATDIAA
                     RGIDIPSIKYVINYDTPRDPLTYFHRIGRTARANSDGKAISLVAIEKISDFEKILRYT
                     KYKVRQLNEEIGIPIPKIQKEFKKHNYLNKNKPFNKWSKHNYKNHKKKNYIYKKSSNR
                     NFRKN"
     gene            1342..1860
                     /locus_tag="VHJ57_01305"
     CDS             1342..1860
                     /locus_tag="VHJ57_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HEX2487203.1"
                     /translation="MPSIDNEESFAKISNLIGGEEYQKVAQALLNTQDATDEEIAGAT
                     GMRINVIRKVLYDMFGKALITGIRVKDEKKGWFVYRWRAKSDQIDNFIDNQKKKILDR
                     LQKRLEYEESSEFYHCGNNECQRIKFDIAIESFFKCPTCNENLAMVDNNYTKEALRYK
                     IDQILSEISTEK"
     gene            complement(1871..2539)
                     /locus_tag="VHJ57_01310"
     CDS             complement(1871..2539)
                     /locus_tag="VHJ57_01310"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HEX2487204.1"
                     /translation="MKFPTDLKVIINSAMTLDGKLSSYTGNSRISSKKDLVRVHKLRS
                     KVDAIVVGINTVLIDNPMLTVRFIRNNRNCPTRIIIDSYGRIPLDSKILKTAATIKTI
                     IVVTKQASKDTIEKIKELGAHVIIVGSKLVNLKKLFAMLYKMGYRKILIEGGGELNWS
                     CLHDGIVNELIITITPIVLGGRNAITLVEGRGYSTISQGIKLKLTKIIQNKNDNEITL
                     YYNN"
     gene            complement(2523..3839)
                     /locus_tag="VHJ57_01315"
     CDS             complement(2523..3839)
                     /locus_tag="VHJ57_01315"
                     /inference="COORDINATES: protein motif:HMM:NF005552.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX2487205.1"
                     /translation="MTFIIRNISLLYGKDLVYINKGFICINKNGIITEAGSEDTYFDK
                     YKFNDTKVYDGTGFLVIPGFINSHTHIADSIGKDISFSSTFNEMIHPVYGIKKKILNK
                     STKEHLKYFIRTSAISMMIKGTISFVDFREGGPEGIELLKDSILDLPIKSVILGRIEQ
                     YFDLEKILEIKKQQTKGNSDDKLVVDKLQKLSLESLSRVLELSDGLGLSGANENTDDA
                     LKQYIHKVKEFNKKNNYLHYRKKIIAIHAAESQSTIKFSKLLTGKTEVERIINFLKPD
                     ILVHMTHATLEDLTLALHNNIGIVLCPRSNGILGNGIPDIVTMIKLGCKISIGTDNIM
                     INSPDLLQELDYLWKVIKSHSSKYSNYIDPKELLKMITVNPGEIFDLNSGSIDIGKYA
                     DILFIDKYHIDLQPIHNIYSSIIHRLSYGAIKAIMINGRFINEIPY"
     gene            complement(3836..4609)
                     /locus_tag="VHJ57_01320"
     CDS             complement(3836..4609)
                     /locus_tag="VHJ57_01320"
                     /EC_number="3.5.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase IIa"
                     /protein_id="HEX2487206.1"
                     /translation="MNIQLTIIKIEGYGPWTLVLGSDREAKLQMLQAKIYHDLQYLFS
                     KYNCLVFSNRFDEYFVITNTLSLEDHVSVRKEISIKHPNLCISFTIGIGKTPLDANIQ
                     AYQIRRNEKFLVKNFQIYGEINNISESPSLNDNKVKILHIDINDSSGFGNHLSPYETT
                     IKVMKSYVLLMDKFLKKDSLTFFLGGDNFMIIAADLLEKDEISKLLNSISDELDIKFN
                     CGIGFGRTSRESAKNATEALDSIRKLRKKGQIIPIYEIK"
     gene            complement(4606..5070)
                     /gene="ribH"
                     /locus_tag="VHJ57_01325"
     CDS             complement(4606..5070)
                     /gene="ribH"
                     /locus_tag="VHJ57_01325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX2487207.1"
                     /translation="MIKVHLAIVVSEFNHEITDVMLEEARVHASKLNSNVSHICYVPG
                     TFDMPLIISHLLQNNTIDAVITLGAVIKGDTNHDIIIAETTARLISDLSLEYKKPVAL
                     GISGPNMTIEQAWERAKIVPIRAVNAAVNMVTRIKKLNESQSSQNKTTIIIK"
BASE COUNT         1851 a          728 c          704 g         1821 t
ORIGIN      
        1 tttcctattc aagaaaaagc aattcctatt attttggagg gaaacgatgt aattggacaa
       61 gcacatactg gaacgggaaa aactgcagca tattcattac ctattttaat gaaaatgaaa
      121 aatgcaagaa atgtacaagc tttagtatta gttcctacaa gggaattatc aattcaagta
      181 gcgagggaaa ttaataaact tgcaaaatat acagaaacaa aaatagctgc tatttatgga
      241 ggacaaaata tgagaaatca aattattaga ctcgaaagag gaattgatat tattgttgcc
      301 actcctggaa gattaattga ccatatagga caaaaaacag tatacttgga tacaatagat
      361 tttgtaatat tagatgaggc tgatcgaatg ttagatatgg gatttataga agatattaat
      421 tatatattat caaaggtaaa acataaaaaa caattatgtc ttttttcagc aacaatattt
      481 ccagagataa tagaaattag taaaaaatat atgaaaaacc cgatacatat tacagtaaat
      541 caaaatgata ttacattaaa aaccatagaa caaatgtatt taattacaaa tgaaaaagaa
      601 aaatacaatc atttgtgtta tttaattaaa cagagtaata ataatcagat ggtaattttt
      661 acttctacaa aacaaaaggc tgaagaacta gctatgaatt taagaaaaaa agaactatta
      721 aaagtaactg cattacatgg agatctgtct caaagacaaa gaaattacgc aatgcataag
      781 tttagaaatg agcaagagca aattctagtg gctacagata ttgcagcaag aggaatagat
      841 atcccttcca taaaatacgt tattaattat gataccccta gggacccttt aacctatttt
      901 catagaatcg gtagaactgc aagagcaaat tctgatggca aggcaatatc tctagttgcg
      961 attgaaaaaa tttcagattt tgaaaagatt ttgagatata ctaaatataa ggttcgccaa
     1021 ttaaatgagg agattggcat tccaatacct aaaattcaaa aagagtttaa aaaacacaat
     1081 tatttaaaca aaaataagcc ctttaataag tggtcaaaac ataattataa aaaccataag
     1141 aaaaaaaact acatctataa aaaatcttca aatcgaaatt tcagaaagaa ttaatttcca
     1201 atttttgaca cataattaag attgttatat aaactcaatt ttcttttaat aactgtttaa
     1261 taaattatag ttaatttaaa atactaaagg gtagaacttc ggtaaatata taatacctga
     1321 atcacaacat atactattgt tatgccatct attgataatg aagaatcttt tgccaaaata
     1381 tccaatttaa taggtggaga agaatatcag aaagttgctc aagcactttt aaatacacaa
     1441 gatgcaactg atgaagaaat tgctggtgca acaggaatga gaatcaatgt tattagaaaa
     1501 gtattatatg atatgtttgg aaaagcgtta attactggaa ttagagttaa agatgagaaa
     1561 aaaggttggt ttgtttatcg ttggcgtgca aaaagtgatc aaattgacaa tttcatagat
     1621 aatcagaaaa aaaaaatact ggatagattg caaaaaagat tagaatatga agaatcatca
     1681 gaattttatc attgtggaaa taatgaatgt caaagaataa aatttgatat agctatagag
     1741 tcttttttta aatgccctac ttgtaatgag aaccttgcta tggtagacaa caactataca
     1801 aaagaagcac tgagatataa aattgatcaa atactatctg aaatatccac agaaaaataa
     1861 taattagtat ttagttatta taataaagcg ttatttcatt atcattttta ttttgaataa
     1921 ttttcgtcag ctttaatttt atcccttgtg atatagtaga atatcctctt ccctcaacaa
     1981 gagtaatagc atttcttcca cctaaaacta ttggcgtaat agttataatt aattcattta
     2041 caattccatc atgtaaacaa gaccaattta attctcctcc tccttcgata aggatttttc
     2101 tataacccat tttgtatagc atagcgaaaa gtttttttaa atttactaac tttgatccaa
     2161 caattatgac atgtgctcct aactccttta ttttttctat agtatcttta cttgcctgtt
     2221 ttgtaacaac aattatagtt ttaattgtag cagcagtttt tagaattttg gaatctaatg
     2281 gtattctacc ataactgtct ataataatac gagtgggaca atttctatta tttcttataa
     2341 atctaacagt taacatagga ttgtctataa gaactgtatt tataccaact acaattgcat
     2401 caactttaga gcgtaattta tgaacgcgta ctaaatcttt cttcgaagag atacgtgagt
     2461 tgccagtata agaagagagt ttaccatcta aagtcatagc agaattaata attactttta
     2521 aatcagtagg gaatttcatt tataaacctg ccattaatca tgatagcttt tattgcccca
     2581 taagaaagtc tatgaataat agaagaataa atattgtgaa ttggttgtaa atctatatga
     2641 tatttatcaa taaataaaat atcagcatat tttccaatat cgattgaccc agaatttaaa
     2701 tcaaatattt ctcctgggtt aacagtaatc atttttaata attcttttgg gtcaatgtaa
     2761 ttagaatatt tagatgaatg tgatttaatt actttccata aataatctag ttcttgtaaa
     2821 agatccggag aattaatcat tatattatca gtacctatcg atatcttaca tcctaatttt
     2881 atcatagtca caatatcagg aattccattt ccaagaattc cgttagatct tggacataat
     2941 actatgccta tgttattatg gagggcaaga gtgagatctt ccaaagtagc atgtgtcata
     3001 tggaccagga tatctggttt aagaaaatta ataatcctct cgacttctgt ttttcctgta
     3061 agtaattttg agaatttgat agtgctttgg gattcagctg catgaatagc aattattttt
     3121 tttctataat gtaaataatt attcttttta ttgaattcct taactttatg tatatattgt
     3181 ttaagagcat catcagtatt ttcgtttgcc ccgcttagtc ctaatccatc acttaattct
     3241 aatactcttg aaagagactc caaagacagt ttttgtaatt tatctactac taatttatca
     3301 tcactatttc ctttagtttg ttgtttttta atttccaata ttttttctaa atcaaaatat
     3361 tgttctattc tccctaaaat tactgatttt ataggcaggt ccagtataga gtcttttaat
     3421 aattcaattc cttctggacc gccttcacga aaatctacaa aagatattgt accttttatc
     3481 atcatggaaa tagcagaagt tcgtataaag tattttagat gttctttggt acttttattc
     3541 aatattttct ttttaattcc ataaacagga tgaatcattt cgttaaaagt agaactaaat
     3601 gatatatcct ttcctattga atctgctatg tgagtatgag aatttataaa accaggtata
     3661 acaagaaatc ctgttccatc ataaactttt gtatcattaa acttgtactt atcaaaatat
     3721 gtgtcttcgc ttccagcttc tgttattatt ccatttttat taatgcatat aaatccttta
     3781 tttatgtaaa ctaagtcttt tccatataat aaactgatat ttctaataat aaaagtcatt
     3841 tgatctcata gataggaata atttgtcctt ttttacgcaa ttttctaatg gaatcgagag
     3901 cctctgtggc attcttggct gattctctag aggttcttcc aaatcctatg ccacaattaa
     3961 atttaatatc caattcatca gagatactat ttaataactt agaaatttca tctttttcta
     4021 atagatcagc agcaattatc ataaagttat ctcctcctag aaaaaaagtt aatgaatctt
     4081 tcttgagaaa tttatccata agaagaacat acgatttcat taccttaata gtagtttcat
     4141 aaggagatag atgatttcca aaacctgatg aatcatttat atcaatatga agtattttta
     4201 ctttgttgtc atttaaagag ggagattctg aaatattgtt tatttcacca taaatttgaa
     4261 aatttttaac aagaaatttt tcatttcttc ttatctgata ggcttggata tttgcatcta
     4321 atggggtctt tccaattcct attgtaaaag atatacacaa gtttggatgt ttaatagaaa
     4381 tctcttttct aactgagaca tgatcttcta atgataatgt atttgtaatt acaaaatatt
     4441 catcaaatct attggagaat acaagacaat tatatttgga gaataaatat tgcaaatcat
     4501 gataaatctt ggcttgtagc atttgtaatt ttgcttctct atcactaccc aagaccaatg
     4561 tccatggtcc ataaccttct attttgatta ttgttagctg gatattcatt ttataataat
     4621 agtagtttta ttttgactag attgactttc atttaatttt tttattctag ttaccatatt
     4681 tacagctgca tttactgcac gtatgggaac tatttttgct ctttcccatg cttgttcaat
     4741 agtcatgtta ggtcctgata tacctagtgc aactggtttt ttatattcta atgataaatc
     4801 cgatatcagt ctcgctgtag tttcagcaat tattatatcg tgatttgtat cgccttttat
     4861 aactgctcct aatgtaatta cagcatcaat agtattattt tgcaataaat gacttattat
     4921 tagaggcata tcaaacgttc caggtacgta acaaatatga ctaacattcg agtttaactt
     4981 tgatgcatga actcttgctt cttctaacat tacatcagta atttcatgat taaattcaga
     5041 aacaactatt gctaagtgaa ctttgatcaa ttgaaattta aatcaccgaa tatgtcttta
     5101 attg
//
LOCUS       DATAFW010000056         3864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1256792, whole genome shotgun sequence.
ACCESSION   DATAFW010000056 DATAFW010000000
VERSION     DATAFW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256792"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VHJ57_01330"
     CDS             <1..213
                     /locus_tag="VHJ57_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX2487208.1"
                     /translation="ENVAVIAVVGSGMRGIKGVAAKTFGAVAKRNINVIMITQGSSEL
                     NLAFVIDDKNCEEAVNVLHQEFINVK"
     gene            285..1043
                     /gene="cobJ"
                     /locus_tag="VHJ57_01335"
     CDS             285..1043
                     /gene="cobJ"
                     /locus_tag="VHJ57_01335"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HEX2487209.1"
                     /translation="MVGVGPGNHDHMTFRAKHVIEESEIIVGYDTYVSLVEDLIEGKE
                     IYRYAMTQEVDRANQAIEFAEDGKIVSLVSSGDPGIYGMIGLIYEILAEKGWNKESGM
                     YVECVPGVSSLNSCGALVGSPLMTDFAVVSMSDLLVPWEMIVKRVEAAAIGDYVTVIY
                     NPASKKRIHQLKDTRDILLKYRKPNTPVAIIKGAYRDSQEIVLTNLDDMLNHSEIIGM
                     ITTIIIGNSSTYNYNNMMINPRGYRSKYQLVKEK"
     gene            complement(1091..2134)
                     /locus_tag="VHJ57_01340"
     CDS             complement(1091..2134)
                     /locus_tag="VHJ57_01340"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX2487210.1"
                     /translation="MDLESKLSLITSEPTEEIVTMNELKNLLQTISKPKHYIGLEISG
                     KLHLGSLILPGFKINDLIKANFNTTVFLADWHTFINNKLEGNWNKINEIAKYYSEAFK
                     FFCPGVNIILGSQLYNEYEEYWKNFMQFCKQITLARTMRSLTIMGRNTNENLDFSQFL
                     YPAMQSTDIHALNLDLVHSGTDQRKIHMLMREVFPKLKWKVPVSLHHHLLPGLTEPVK
                     LGLTEDSTEDLRISSKMSKSKPMSGIFIHDDDKTIETKIKKSFCPLGFSENNPILEII
                     RYVILHSFKEFIIERPSKYGGTISYDNYSQLKEDYDKQKIHPQDIKQSTSLYICKLIS
                     PIRNHFKNNEPLV"
BASE COUNT         1378 a          521 c          606 g         1359 t
ORIGIN      
        1 gaaaacgttg cagttattgc agtagttgga tcaggtatga gaggaattaa aggagtcgca
       61 gctaaaacct ttggagcagt agcgaaaaga aatattaatg taataatgat aacacaagga
      121 tcttcggaac ttaatcttgc atttgtcatt gatgacaaaa attgtgagga agcagttaat
      181 gtgcttcatc aggaatttat aaatgttaaa tgatatgata tagcagcaaa aagataaatt
      241 ttgtctttac cagtatatac aagttgtcaa agggaaaatt gtatgtggta ggtgttggac
      301 caggtaatca tgatcatatg acatttagag caaagcatgt aattgaagag agtgaaatca
      361 tagtgggata tgacacttat gtttcgcttg tagaggattt aattgaagga aaggagatat
      421 atcgatacgc gatgacacaa gaggtagaca gagctaatca agcaatagaa tttgctgaag
      481 atggaaaaat agtttcacta gtttcctctg gtgatccagg gatctatgga atgataggtt
      541 taatttatga aattttagca gagaaaggat ggaataaaga aagtggaatg tatgttgaat
      601 gtgtacctgg tgtttcttca ttaaattcgt gtggggcctt ggttggttct ccattaatga
      661 ctgattttgc agtagttagt atgagtgatc tattggtacc gtgggaaatg atagttaaaa
      721 gagtggaagc agcggcaatt ggggattatg ttactgtaat ttataatcca gcaagcaaaa
      781 aaagaattca tcaactaaag gatacgagag atattttatt aaaatatagg aaaccaaata
      841 ctcctgttgc aataataaaa ggagcctatc gcgatagcca agagatagta ttaacaaatt
      901 tagatgatat gttaaaccat agtgaaatta ttggaatgat aactactata attataggaa
      961 actcatctac atataactac aataatatga tgatcaatcc aagaggatat agatcgaaat
     1021 accaattagt aaaagagaag taaaaataga gataatttag cctcctactt aattagattc
     1081 gtaaaatatt ttacactaat ggttcattgt tcttaaaatg atttctaatt ggtgagatta
     1141 gtttgcatat atataatgaa gtggattgtt taatgtcttg aggatgaatt ttttgtttat
     1201 cataatcttc ttttaactgg gaataattat cataagagat agttcctcca tattttgaag
     1261 gacgctcaat tataaattct ttgaaactat gcagtataac atatctgata atttcaagaa
     1321 tgggattatt ttctgagaaa cctaaaggac aaaatgattt cttaattttc gtttctattg
     1381 ttttgtcatc atcatgaata aagattccac tcattggctt acttttactc attttgctgg
     1441 aaattcttaa atcttctgta ctatcttctg ttaatcctaa cttgacaggt tcagtgaggc
     1501 caggaagtaa gtgatgatgt aaactgactg gaactttcca tttcaatttt gggaaaactt
     1561 ctctcataag catatggatt tttctttgat ctgtacccga atgtacaagg tctaaattaa
     1621 gggcatggat atctgttgac tgcatagcag gatatagaaa ttgagaaaag tcaaggtttt
     1681 cgtttgtatt tctccccata attgtcaagg atctcatagt tcttgctaaa gtaatttgtt
     1741 tgcaaaattg cataaaattt ttccaatatt cttcatattc attataaagt tgacttccaa
     1801 gaatgatatt tacgccagga caaaaaaatt tgaaggcttc agaataatat ttagctattt
     1861 catttatttt attccaattt ccctctaatt tattattgat aaaagtatgc caatcagcca
     1921 aaaaaactgt agtattaaaa ttcgctttta ttaaatcatt tatcttaaag cctggtaaaa
     1981 taagactacc caggtggagt ttacctgaaa tttctaaacc gatatagtgt ttaggttttg
     2041 aaatagtctg taatagattc tttaattcat tcattgtaac aatttcttct gttggttcag
     2101 aagttataag tgataacttt gattctaaat ccatataaac tttcgatata ttaacagtaa
     2161 ttaaatttta catcattgat aattaattaa ggaaataata ttaaattctt gaatacttat
     2221 caaaaaatgt tattaaatga agtagtacta aatttaagta attcttcttt atttgaatcc
     2281 agtctttttt cgttagatcg gataaagtaa tgacacatta aacttctttg aaattcatat
     2341 taaacaaaag ttatgtatat tgtgtcaaat tatattttcc tccattttaa aactgtataa
     2401 attttaggct ctcgagttaa gggattaatg cctttcttta actcaaaatc attattatct
     2461 gttatagtat taatattcat tgaaacaaaa gaattgtatc cagcttcgta tacacattaa
     2521 gttgcattca attttcttcc ttttaaatat aaatagatac taatcatcga aactagcgag
     2581 tcttattcga ccatgtaata gtcatacccc taacgtgtgt aaaactagat atagaaatgg
     2641 caccaacctg tcagtatatt atatattata ctttgtatta tccatcaaat tattatccaa
     2701 actttaaata tttcggtata cctgttttgt tactactgtt ggtatacatt ttagttgctc
     2761 ttaatttaag agtaattgat ctcctctttt agcagaaaca gaattttggt tattgtaacg
     2821 gtactattat atgcataaaa gtacagtcta aggtaattgt taaatgatct aaatcacaat
     2881 tcttattttt gttactacaa atttagttat cttcaaagtt ctttctattc ttaaagtata
     2941 acattccctt ttctattaaa ctttttttat catagtgaat acaatctatg tttcaaatct
     3001 agaaaattaa atgcttgagg atactacgta ccatcatcaa tctatacgat atgttttcca
     3061 tatttttgca ctactgatcg tgaaattatc tcatacaaaa tgtttccttg ttcagaactg
     3121 tagagtattt acagatttgt gagcctatcc aattgtaatc cataatcaat aatgctgatg
     3181 tccaatttga atgacggttt catctattat tgatgcagct actcttgtct ttatgtaaat
     3241 ttgataagaa ctaattctct atagccatat acaaacagaa agataccttc tttcacaatg
     3301 gatgtattta ttaaactcaa accaaaccat gactattcaa atacaaagaa tacataacta
     3361 caattgtgga tatggtattt ttgatagatt tgactaacat tccttattaa gttaagacct
     3421 tcacaaatag atatttccta agtaaacagc ccatgatttt ataatgttcg ttgttttttt
     3481 aatagttcaa aataaattga ctatggctcc tttacatttg tgtaaattat aagaaaagta
     3541 atttataaaa atccctcaca tcccaaaatt tatgaatatg agtaaacatc aaaaatggaa
     3601 aaatacaaga tagggttcta aaaaatattc aaaatttacc gttcgtaata caatatccca
     3661 caagattacc tagaacatta aagataattg atgaaagcac aagaatatta gaattggtag
     3721 taggtaaaaa ataagaatat aaaggctcgt aaaaatattt tccttgtaaa aaatgaaatt
     3781 attagaatat caaggaaagg aactttttaa tcattatggg atcaacgtgc caaaatcata
     3841 tttggcaaaa aatcttgaag aagc
//
LOCUS       DATAFW010000057         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3900250, whole genome shotgun sequence.
ACCESSION   DATAFW010000057 DATAFW010000000
VERSION     DATAFW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900250"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..903)
                     /locus_tag="VHJ57_01345"
     CDS             complement(361..903)
                     /locus_tag="VHJ57_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2487211.1"
                     /translation="MMESELTLPINSNRDHLLGPQKAPVTLLEYGDYECPYCRQANLI
                     VAKTVQQFGNKMRFAFRNFPLSQIHPHAQHAAEAAECAGAQNRFWEMHDILYENQQAL
                     EDVNLLEYAKVLELDMSRFQDDFYNHTFALRVREDFLSGIRSGVNGTPTFYINGMRYN
                     DSWDQETLTKTLKDIINKNV"
     gene            complement(903..1430)
                     /locus_tag="VHJ57_01350"
     CDS             complement(903..1430)
                     /locus_tag="VHJ57_01350"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2487212.1"
                     /translation="MSTQTEQQKQISNALQPGTSAPNFTLRTTPDQSVSLDQFRGNPV
                     IILFYPADFSPVCGDEVSLYNEILPEFQRFNAEVIGISVDGVWCHLAFSKHKKLRFPL
                     LSDFEPKGNIAQKYGVYRKNDGVTERALFLVDKDGIIRYSYVSPIGVNPGADGILKAL
                     ESLPQSTEQKGSTPT"
     gene            complement(1490..2170)
                     /gene="hsp20"
                     /locus_tag="VHJ57_01355"
     CDS             complement(1490..2170)
                     /gene="hsp20"
                     /locus_tag="VHJ57_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp20"
                     /protein_id="HEX2487213.1"
                     /translation="MSFPNNLDPYNFFRQLFGGSSSSGERKGREGRGDFFGGSGRWNF
                     DDMFREFDEMRREMSRTFSEQFKNIENKVPKDLIKEYETPEGAKVREVGPIVYGYSMT
                     IGPDGKPNIQEFGNVKSFSGRGSFEQPSLSAEREPLIDISSTDKEVKIVAEMPGISKE
                     KIKIDAYDKYVEIKSEDPNRKYHKKIEVPEDIDINSARSNYNNGVLEINFKKKEQQKP
                     KGRQINVE"
     gene            2846..5125
                     /locus_tag="VHJ57_01360"
     CDS             2846..5125
                     /locus_tag="VHJ57_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX2487214.1"
                     /translation="MTSNLDNGKKPNEGATLKVAEAEQRDVGRKIARVDPEIVERLHI
                     TTGDALELSSLGKKTTVLSWPAKESDRGKGIIRIDGLIRNRLDVGINDLIEIRGVESK
                     DAKSITFAPTEPLRITGGEQYLAEYLNGTLMTKGDTIPINVMGRRIDLVVISTNPSGP
                     VIINDSTEIVVSEESSKAIQISKEGVAASITYEDIGGLGDAVARVREMIELPLRHPEL
                     FKRLGVEAPKGVLLHGPPGTGKTLLAKAVANETNSTFYTIGGPEIMSKYYGESEEKLR
                     SIFEQAEKNAPSIIFIDEVDSIAPKRDEVSGEVERRIVAQLLSLMDGMTSRGKVVVIA
                     ATNRVNAIDPALRRPGRFDREIEIGVPNRDGRLEVLQIHTRGMPMEKDVNLEKLADIS
                     HGFVGADLQALAKEAAIRALRRVLPEINLSSESIPLDILRKIVVRMQDFMDVIKEMEP
                     SAMREVFVEVPDIKWEDIGGLSSIKQELQEAVEWPLKYNQIFTYSDAIPPKGILLYGP
                     PGTGKTLMAKASANESEANFISVKGPELLSKWVGESEKGIREIFRKARQAAPCIIFFD
                     ELDAITPARGGSFGDSHVTERVISQFLTELDGLEILTNVVVIGATNRPDIIDPALLRP
                     GRFDRILYVPPPDRDSRLQIIKIHTKKKHLAEDVNIDQLADDTDGYTGADIASLSSAA
                     VMLALREHVSKYKDPKEAEEHKEEVKIHRTHFEEAMKKIRPLSMQELNMYKTVSEQFG
                     KPQMSRAETADKKSKDVYV"
     gene            5122..>6296
                     /locus_tag="VHJ57_01365"
     CDS             5122..>6296
                     /locus_tag="VHJ57_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2487215.1"
                     /translation="MKPVLLTVDDDPLVLVALERDLKQQYSNRFLLLQADSGLKGLEL
                     IKQLKLRSESVALFLVDQRMPQMTGVEFLEHALNTYPEAKSILLTDYGDTDSVIKSIN
                     KVRIDYYLTKPWDPVEVHLYPVLNELLDDWWASCHPPFEGIKVIGLRWSPRSHEIKDF
                     LARNGIPYQWLDIEGDEEARRLVSIINSTWSDISTTNNNNNSDTFSFNSDASSSSSSS
                     SISSSIHLPLVIFPDGSYISEPTNSQIAEKVGLKTRAQMAFYDLVIIGGGPAGLAAAV
                     YGSSEGLHTLLVERQAPGGQAGLSSRIENYLGFPSGLTGGDLARRAVTQAVRFGTEIL
                     DPQEVVDIRVEDPFRIVKLADGTEIRCHVMLIACGVTYRKLDDVKGIDKLVGAGVYYG
                     "
BASE COUNT         2136 a          986 c         1102 g         2072 t
ORIGIN      
        1 ctattacaga ttatttctcc cgattcaaca tcagtaatca ttctggattc actaggatca
       61 catatttcac actgaattac ggattgcata ttttttttgt atttattata ataatagaca
      121 atcaaatata taaatattat acctcttttt ataagattta cataagtaaa tcttagtatt
      181 aatttaacaa catgaattat taaaaatata gcatgtatta ttatactgta atatttctta
      241 ctgtttctcc ttcaaatcaa agataagtgt ttaatagaga aatttgtata tcatgagcag
      301 tttgaatgtg atatcatatt aatggcagct atgactaatc agtattaata attaatataa
      361 ttatacgttt ttattaataa tatctttaag tgttttagtc agagtttctt gatcccatga
      421 atcattgtaa cgcattccat taatataaaa tgttggagta ccatttacac cacttcttat
      481 cccactaaga aaatcttcac gtaccctcaa tgcaaaagta tggttataga aatcatcttg
      541 aaatctagac atatctaatt ctaatacctt ggcatattct aataaattta catcttctag
      601 ggcttgttga ttttcatata atatatcgtg catttcccaa aacctattct gagcacctgc
      661 acattcagca gcctcagcag catgttgagc atgaggatgt atttgactca atggaaaatt
      721 gcggaatgca aagcgcatct tatttccaaa ttgttgaacc gtcttggcta ctattagatt
      781 agcttgtcta caatagggac attcataatc tccatattca agtaaagtta caggtgcctt
      841 ttgtggtccc agtaaatgat ctcgattact gtttataggt aaagtcagtt ctgattccat
      901 cattatgttg gtgtgcttcc tttttgttct gtagattgag ggagtgattc taaagccttc
      961 aatatgccat cagcgccggg attgacccca attggtgaaa catagctata tctaattata
     1021 ccatctttgt caactaagaa taaagctctt tctgtgactc catcattttt gcgataaaca
     1081 ccatattttt gtgctatatt tcctttaggt tcaaaatctg aaagcaacgg aaagcgtaat
     1141 tttttgtgct ttgaaaaagc aagatgacac caaacaccat caacagatat tccaatgact
     1201 tctgcattga agcgttggaa ctcaggcaga atttcattat ataaagagac ttcatctcca
     1261 catacaggag aaaagtcggc tggataaaat aaaataatta ccggatttcc cctaaattga
     1321 tccaaagata cagattgatc tggagtagta cgtaaagtaa agtttggtgc actagtacca
     1381 ggttgtagtg cattggatat ttgtttttgt tgttctgttt gagtagacat attgttaagt
     1441 agtattcata taatatatgc atttaaacaa attagtataa tattcctact tattcaacat
     1501 taatttgtct gccttttggt ttttgctgtt ctttcttttt gaagtttatt tcaagaactc
     1561 cattgttata atttgatctt gcactattta tatcaatatc ttctggcact tctatttttt
     1621 tgtgatattt tctattagga tcctcacttt ttatttctac atatttatca tatgcatcta
     1681 tttttatttt ttctttactt attccaggca tctctgctac aattttaacc tctttatctg
     1741 tagatgagat atctattaat ggttctctct ctgctgaaag tgatggttgt tcaaaggatc
     1801 ctctcccaga aaaagattta acgttaccaa attcttgtat atttggcttt ccgtcaggcc
     1861 ctatggtcat agaatagcca tatactattg gaccaacttc tctaactttt gcaccttctg
     1921 gtgtttcgta ttctttaatt agatcttttg gcactttatt ctcaatattt ttaaattgtt
     1981 cagaaaatgt tctactcatt tctcttctca tttcatcaaa ttctctaaac atatcatcaa
     2041 agttccatct tccgctacct ccaaaaaaat ctcctcttcc ttcacgtcct ttacgttcgc
     2101 cactactact gctaccgcca aacaattgtc taaagaaatt gtatggatct aaattatttg
     2161 gaaaactcat aaattataat gaattacttt ctatattaaa gttatagaaa atgttctgtt
     2221 ttaagttaca ataaaaaata ctttaaagaa aattaaagct ttaactgtaa aacaattaat
     2281 tttagattaa ttgactaaat aagttgtcca ctatatatgc acaattacat ttaatactca
     2341 ttttgtattg ctaattaaaa ataagttcct ttaatagtat aaaatatgac cttattaata
     2401 caactaacag ttattcacaa tattatttaa tacaaatagg ttagaaaata tacacactgg
     2461 atctggatat atagatttat gcatttcact atactataga tttattttta ccatcgttat
     2521 aatttttcta ttaaatttgt attgagaatg attaattata ctgtcatact gactaatatg
     2581 aaaaataaaa tttttaatct ttgaaattta ttatttgtac tcttaaaagt ttcaataaaa
     2641 tatacctatt tatttaaatg accattatta tactatattt gagatttttt accgtatctt
     2701 tataaatact ataacaataa aaattgttac tataagaaga tattctattg tttttaaatt
     2761 aacaaaaaaa cgttaacaat aattaaaatc ctaaataata cagctaacct ttaagttgtt
     2821 tagtgtaaat catattttat aagatatgac atcaaacctt gataatggta aaaaaccaaa
     2881 tgaaggtgca actctaaagg ttgctgaagc agaacaacgt gatgttggaa gaaaaatagc
     2941 cagagttgat cctgaaatag ttgaacgtct acacattaca acgggtgatg cattagaatt
     3001 atcctcactt ggtaaaaaaa caacagttct cagttggcct gcaaaagaaa gcgatagagg
     3061 caagggtatt attcgtatag atggactaat tagaaataga ttagatgttg gtattaatga
     3121 tttaatagaa atcagaggag tcgaatcaaa agatgctaaa agcataacct ttgcccctac
     3181 cgaaccattg agaataactg gtggagagca gtatcttgct gaatatttaa atggtacact
     3241 aatgaccaaa ggcgatacaa taccaattaa tgttatgggt agaagaatag atttagttgt
     3301 tatttctacc aatccttctg gtccagttat tattaatgat tctacggaaa tcgtagtatc
     3361 tgaagaaagc tcaaaagcaa tacagatatc aaaagaagga gtagctgcat ctattactta
     3421 cgaagatatt ggaggattag gtgatgcagt tgcacgagta agagaaatga ttgaacttcc
     3481 attaagacat cctgaacttt ttaagagatt aggagtagaa gctccaaaag gtgtattgtt
     3541 acatggtcct ccgggtacag gtaaaacatt gctggcaaaa gcagtggcta acgaaacaaa
     3601 ctccaccttt tatactatag gtggtcctga aataatgagt aaatattatg gagaatcaga
     3661 agaaaaacta agaagtatct ttgagcaagc agaaaaaaac gcacctagta taatatttat
     3721 cgatgaagtt gattctatag cacctaaaag ggatgaagta tcaggagaag tagaaaggcg
     3781 cattgttgct caactgttat cattaatgga tggtatgact tcaagaggga aagttgtagt
     3841 aatagcagct acaaataggg ttaatgctat tgatcctgct ttaagaaggc ctggcagatt
     3901 tgatagagaa atagaaatag gtgttccaaa cagagatgga agattagaag tgctgcagat
     3961 acatacaaga ggaatgccta tggaaaagga tgtgaattta gaaaaactgg cagatatctc
     4021 tcatggtttt gttggagctg acttacaagc attggcaaaa gaagctgcca ttagagcatt
     4081 gagaagagta ttacctgaaa tcaatctttc tagtgaaagt attccattag atattttaag
     4141 aaaaatagtt gttagaatgc aagattttat ggatgttatt aaagaaatgg aaccttccgc
     4201 gatgagagaa gtatttgtag aagttcctga tataaagtgg gaggatattg gcggcctttc
     4261 ttcaataaag caagaattac aagaagcagt tgaatggcca ctgaaatata accaaatatt
     4321 tacatattcg gatgcaatac ctccaaaagg tatcttactg tatggaccgc caggaacagg
     4381 taaaaccttg atggctaaag cttctgcaaa cgaaagtgaa gctaatttta tcagtgtaaa
     4441 aggtcctgag ctattatcaa aatgggtagg agaatccgaa aaaggaataa gagaaatatt
     4501 tagaaaggca agacaagcag ccccttgtat tatattcttt gatgaactgg atgcaataac
     4561 gcctgctcga ggtggaagtt tcggtgattc acatgttact gaaagagtaa taagtcaatt
     4621 tttaacagaa cttgacggat tagaaatttt aaccaatgta gtcgttattg gtgcaacaaa
     4681 tagaccagat attattgatc ctgcattatt gaggcctggt agatttgata ggattcttta
     4741 tgtcccacca cccgatcgtg attctaggct acaaataata aaaattcata ctaaaaagaa
     4801 acatttagca gaagatgtta atattgatca attggcagac gatacggatg ggtatactgg
     4861 agctgatatt gcttcgctat catctgcagc tgttatgctt gctttacgag agcatgtatc
     4921 aaagtataaa gatccaaagg aagcagaaga acacaaagaa gaagtaaaaa tacataggac
     4981 acattttgag gaggcaatga aaaaaatacg acctttatca atgcaagaat tgaatatgta
     5041 caagacagta tctgaacaat ttggaaaacc tcaaatgtca agagccgaaa cagcagataa
     5101 aaaaagtaaa gatgtatacg tatgaagcct gtattgctga ccgtggatga tgatccacta
     5161 gtattggtag ctcttgaacg agatttaaaa cagcaataca gtaatcgttt tctcctactc
     5221 caagccgatt ctggtctcaa gggtctagaa ttaataaagc aattaaaatt acgcagtgaa
     5281 tctgttgcat tatttttagt tgaccaaaga atgcctcaaa tgactggggt tgaatttctg
     5341 gaacacgctt tgaacacata tccagaagca aagagtattt tacttacgga ttatggagat
     5401 acagattctg taataaaatc tattaacaaa gtaagaattg attattactt gacgaaacct
     5461 tgggatccag tggaagttca tctttatcca gttctcaacg aattactaga tgattggtgg
     5521 gcttcatgcc accctccttt tgaaggtata aaggttatag gtcttagatg gtcaccaagg
     5581 tcacatgaga taaaagattt tctggctaga aatggaatac cttatcaatg gttagatatt
     5641 gaaggagatg aagaagcacg tcgtttagtt tccatcatta attcaacttg gtcagatatt
     5701 tctactacca ataataataa taattcagat acattttctt ttaattcaga tgcatcatca
     5761 tcatcgtcat cctcatcaat ttcttcttct atacacttac cgcttgttat ttttcctgat
     5821 ggctcataca tatcggaacc aaccaactcc caaattgcag aaaaagttgg tctcaagact
     5881 cgtgcacaga tggcttttta tgatttggta attataggag gaggcccagc agggttagct
     5941 gcagctgtat atggatcatc agaaggacta catacattgt tagttgagag acaagctcca
     6001 ggaggacagg caggattgag ctctagaata gaaaattatc ttggatttcc ttccggtttg
     6061 actggtggtg atctagctag acgtgctgtt actcaggctg ttcgatttgg tactgaaatt
     6121 ttagatccac aggaagttgt agatatacga gttgaggatc catttcgtat agtcaaattg
     6181 gcagatggaa cagagataag atgtcatgta atgcttattg cttgtggagt gacatatcgt
     6241 aagcttgatg atgtaaaggg aattgataaa ttggttggag cgggtgttta ttatgg
//
LOCUS       DATAFW010000058         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4780259, whole genome shotgun sequence.
ACCESSION   DATAFW010000058 DATAFW010000000
VERSION     DATAFW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4780259"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VHJ57_01370"
     CDS             <1..408
                     /locus_tag="VHJ57_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2487216.1"
                     /translation="AVYAFSTEAKKVKCWLVKPPKIPWSVLNARRLAQIRASGGTPLA
                     EIYSILLPFIKSFKPEIMITLTDGEPSNYDAVKEMVLIYRNLSIHMAALGLGKDLNDL
                     INIGHNLKYLNYNKTLASRVEDIPKKVIKLLKV"
     gene            complement(438..1106)
                     /gene="fsa"
                     /locus_tag="VHJ57_01375"
     CDS             complement(438..1106)
                     /gene="fsa"
                     /locus_tag="VHJ57_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX2487217.1"
                     /translation="MKIFLDTANIASIRHYSEMGMVDGITTNPTLLSKEGGDPEEIIR
                     EIIKIVQGPVSLEVISTEINQIMEEAYILQRYGSNVVIKVPMIPDGLKAVKKLKEEGI
                     ETNVTLVFSANQALLAAKAGASYVSPFIGRLDDIGNEGMSLIRDINQIFKNYQYNTKI
                     LVASIRHPIHVIEAAKLGADVVTLPPDILGKMFLHPLTDKGLKVFISDWENLKKTNPS
                     IKIL"
     gene            complement(1103..2080)
                     /locus_tag="VHJ57_01380"
     CDS             complement(1103..2080)
                     /locus_tag="VHJ57_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEX2487218.1"
                     /translation="MNLTDFELRKGDMRSAYGSALVDLGTINKDIVCVGADTTDSLKT
                     KKFGDQFPERFFNIGIAEANLVSIAAGLAIAGKTAFASTYAAFLPGRCLDQIRNAICY
                     PSLDVKLVVSHAGLTVGPDGASHQQIEDLAIMRSIPNIRVLAPADSVSVFKIIKNIAE
                     IPGPFYVRLARPSSAIIYDDSDSIFQIGKANILQDGSDITIIGCGLMVYKALKTAKIL
                     KERYNISARIIDSYSLKPLDNDMVLKAGRETGAIITVEEHNIIGGLGSAVAEICSEYH
                     PCYVKRVGVKDTFGESARDSELDILLECYGLTEKEIERAVLSTFDKLKK"
     gene            complement(2061..3698)
                     /gene="rpe"
                     /locus_tag="VHJ57_01385"
     CDS             complement(2061..3698)
                     /gene="rpe"
                     /locus_tag="VHJ57_01385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX2487219.1"
                     /translation="MIFHMRYLDLKRKIQEIRINAIEGIAEAGSGHPGASFSAAEIMG
                     VLYFRELRHDPSNPTWAGRDFFINSKGHSAPGYYATLAVAGYFPKNEMKDLRKFGSRL
                     QGHPVRYSKLLEHHSLPGVEYSGGSEGIGLSVSIGIALANVLDNKENNVYVLLGDGET
                     NEGQVWEAAMTAPKFKLDNLVAILDRNKIQQDGFTEDIMPLDPVRDKWVAFNWNVLEI
                     NGHKVEQIMDALNKASEIKNKPTIIIANTVKGNGIKHMANNPQWHGKAPPNMHIPVLI
                     EELEGEYMIAPSIIAGEKENYEEKIKKVERGGADLIHLDVMDGIFVPNITFLADTIKK
                     LRPITNIPFDAHLMIEKPYDHIEEYINAGCDIITIHAETCDNEKFCYIRDKVMSAGIS
                     LGIAINPQTDLPAWAFSYLNDIDVIIVMSVNPGFSGQKFIPDITSKISNLNMTLKMEG
                     YKGYIEADGGIDASTVKRVYDAGARIMVAGSAVFSNSDINTAILQLKHKTNVTLERNL
                     LKKADENSIRKDWIIARKHILIPVANELGIEDELNRL"
     gene            3845..>5122
                     /locus_tag="VHJ57_01390"
     CDS             3845..>5122
                     /locus_tag="VHJ57_01390"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX2487220.1"
                     /translation="MLKIIADETKISEKRTELLVIGCFFNEDYSDLLNKFDDEISVAG
                     KVIISHIQKFGVLNELYTFGKIPARKMLFAGLGNKNEFNTEKSRIISGKIIQYAKEIK
                     IKELSIIFNFSKDMIESFVEGILLSSYTFENFKTSKNNNTIDKVSILIESNSKEIQKK
                     INETSVICQSVNLGREISNLPPNECSPDYLAKIALDLKHQGIKPKILSLDSMKDIGLN
                     GIVSVGGGSINTPKLIVLKYEGSKKEENPILLVGKAVTFDSGGISIKPSEKMEEMKFD
                     KCGGVNVIAIMKSLSALNIKHNVIGIIPAVENMPSNSSYRPSDIIKMYNGKSVEVINT
                     DAEGRLILADALAFGIKQYKPKLIIDMATLTGACIIALGSNIAGIIGNDEKLTKKVLD
                     ISKVTDEKMWELPLLEDHHEQIKSKNADIKNIGG"
BASE COUNT         1760 a          866 c          776 g         1720 t
ORIGIN      
        1 gcagtctatg catttagtac tgaagcaaaa aaagtaaaat gttggctagt gaaaccaccg
       61 aaaattccat ggtctgtatt gaatgcaaga agattagctc aaataagagc aagtggtggt
      121 acaccattag ctgaaatata cagtattctt ttacctttta tcaaatcatt caagcctgag
      181 ataatgatta ccctaactga tggagagcct tcaaattatg atgccgtgaa agaaatggtt
      241 ttaatataca gaaatttgtc tatccatatg gcagccctag gattaggaaa agatttaaat
      301 gatcttatta atattggtca caatttaaaa tatcttaatt ataataaaac tctagctagt
      361 agagtagaag atataccaaa aaaagtaatt aagttgctca aagtgtaatt gttcacaaaa
      421 taaggataaa taaaaagtta taaaatctta atggagggat ttgttttctt aagattttcc
      481 caatctgaga taaatacctt taagccttta tctgtaagag gatgtaaaaa catttttcca
      541 agaatatcag gtggtaaagt tactacatct gcaccaagtt tagcagcctc aatcacgtga
      601 attggatgac gtatacttgc cacaagaatt ttagtattat attgataatt tttgaaaatt
      661 tggtttatgt cacggataag gctcatgccc tcattcccta tatcatcaag tcttccaata
      721 aatgggctta catatgatgc tcctgccttt gcagctaata atgcttgatt agctgaaaaa
      781 acaagagtaa catttgtttc tattccttct tcttttaatt ttttaactgc ttttaatcca
      841 tcaggaatca tgggaacttt aattacaaca tttgaaccat atcgctgtag aatataagcc
      901 tcttccatta tttgattgat ttcagtagat ataacctcta ggctaactgg tccttggaca
      961 atctttatga tttctctaat gatttcttcc ggatcaccac cttcttttga aagaagcgta
     1021 ggattagttg ttattccatc aaccattccc atttcagaat aatgacgaat ggaagcaata
     1081 ttagcagtat caagaaatat tttcattttt ttaatttatc aaatgtagat agcacagccc
     1141 gttctatttc tttttcggtt agaccataac actcaagcaa aatatctaat tcagaatctc
     1201 ttgcagattc tccaaatgta tctttaactc caactcgctt tacataacaa ggatgatatt
     1261 cagaacatat ctccgctact gcactgccta atcctcctat tatattatgt tcttcaactg
     1321 ttataattgc accagtttct cgtcctgctt tcaacaccat atcattatct aatggcttta
     1381 gtgaataaga atcaattata cgagcagata tattgtatct ttctttcaat attttagctg
     1441 ttttaagagc tttatatacc atcaatccgc aaccaataat agttatgtct gaaccatctt
     1501 gcaaaatatt tgctttacct atttggaata tactatcact gtcatcgtaa attattgctg
     1561 acgagggacg agcaagtctt acataaaatg gacctggaat ctctgcgata tttttaatta
     1621 ttttgaaaac agatacagaa tcagcaggtg cgagaactct tatgttaggt attgatcgca
     1681 taatagctag atcttcaatt tgctgatgtg aggctccatc tggaccaact gttaaaccag
     1741 catgtgatac tactaattta acatctagac taggatagca tattgcatta cgaatctgat
     1801 ctaaacatcg tccaggtaaa aatgcagcat aggtgcttgc aaatgctgtt tttccagcaa
     1861 ttgctaaacc tgctgcaata gaaacaagat ttgcctcagc tattcctata ttaaaaaaac
     1921 gttcgggaaa ttgatcccca aatttttttg tttttaatga atcagtagta tctgctccca
     1981 cacaaacaat atctttatta attgtaccta aatcaaccag agcactccca tatgcagatc
     2041 gcatatcccc ttttcttaat tcaaagtcgg ttaagttcat cttctattcc caactcatta
     2101 gcaacaggta tcaaaatatg tttacgggca ataatccaat cttttcgtat actattttca
     2161 tcagcttttt ttaatagatt tctttctaaa gtgacattag ttttatgttt taactgtaga
     2221 attgctgtat ttatatctga attagaaaaa actgcgctac ctgctaccat aattcgtgcg
     2281 ccagcatcat atactcgttt aacagtagaa gcatcaattc ctccgtctgc ttcaatatat
     2341 cctttgtatc cctccatttt taaagtcata ttcaagttgc taatcttaga ggttatatca
     2401 ggaataaatt tttgtccaga aaagccagga tttacagaca tgactataat gacgtcaata
     2461 tcattcaaat aagaaaatgc ccatgctgga agatcagttt gtggattaat agctatacct
     2521 aaacttattc ctgcagacat taccttatct cgaatatagc agaatttctc attatcacaa
     2581 gtttcagcat gaattgtaat aatatcacaa ccagcattga tatattcctc aatatgatcg
     2641 tatggttttt caatcattag gtgcgcatca aagggtatat tcgttattgg tcttaatttt
     2701 ttaattgtat ctgctaaaaa agtaatatta ggaacgaata ttccatccat cacatctaaa
     2761 tgtataaggt ctgctccacc tctttcaact ttttttattt tttcttcata attttctttt
     2821 tcccctgcga ttatcgatgg tgctatcata tattctcctt ctaattcttc gattaatact
     2881 ggaatatgca tattaggagg tgctttacca tgccattgag gattattagc catatgttta
     2941 atcccatttc cttttacggt atttgcaatt ataatagtag gtttattctt tatttcacta
     3001 gctttgttca atgcatccat tatttgttca actttgtgtc cattaatttc taatacattc
     3061 cagttaaaag ccacccattt atcacgtact ggatcgagag gcattatatc ttcggtaaat
     3121 ccgtcctgtt gaattttgtt tcgatccaaa attgctacaa gattatctaa tttaaacttt
     3181 ggagcagtca tagcagcttc ccatacttga ccttcatttg tctcaccatc acctaataaa
     3241 acatacacat tgttctcttt attatccaga acatttgcca aagcaatacc aattgaaaca
     3301 gaaagaccta taccttcgga gcctccagaa tattctactc ctggtagaga atgatgttct
     3361 aaaagttttg aatatcttac aggatgacct tgcaatcttg atccaaattt ccttaagtct
     3421 ttcatttcat tttttggaaa atatccagct actgccaatg ttgcataata acctggagct
     3481 gagtgtcctt ttgaattaat aaaaaaatcc cttcctgccc atgtgggatt tgaaggatca
     3541 tgacgtaact ccctaaaata taataccccc attatttcag ctgcagaaaa agaggcgcct
     3601 ggatgtccgg atcctgcctc agcaattcct tcgatagcat taattctaat ctcttgaatt
     3661 tttcttttta gatctagata acgcatatga aatatcatgt atcaatacct tcaaaccaca
     3721 aatgtggcat agagaaacaa aatataaaga aagataaaaa tctagctgga gaaataataa
     3781 attattcgca ctaatttaaa ctcaattcaa taatttaaaa tattattgct gaatttcata
     3841 atgtatgtta aaaattatcg cagatgaaac aaagataagt gaaaagagaa cagaactttt
     3901 ggttattgga tgttttttca atgaagatta ttcagattta cttaataaat ttgatgatga
     3961 aataagtgtt gcaggaaaag taattatttc ccatattcaa aaatttggtg tattaaatga
     4021 attatacaca tttggaaaaa tcccagctag aaaaatgtta tttgcaggat taggtaacaa
     4081 aaatgaattt aatacagaaa aatcaagaat tatttctgga aaaataattc aatatgctaa
     4141 agaaattaaa ataaaagaac tttccattat atttaatttc tctaaagata tgattgaatc
     4201 ctttgtagaa ggaattttac tttcatctta tacatttgaa aattttaaaa cttccaagaa
     4261 caataatact atcgataaag tttctatatt gatagaatca aattctaaag aaattcaaaa
     4321 aaaaatcaat gaaacatctg tgatatgtca atctgtaaat ttaggtagag aaatcagtaa
     4381 tttaccacct aatgaatgtt ctccagacta tcttgcaaaa atagcattgg atctaaaaca
     4441 tcaaggaatt aaaccgaaga ttttaagtct cgattctatg aaagatatag gattaaatgg
     4501 gatagtttct gtaggaggtg gtagtatcaa taccccaaaa ctaatagtat tgaaatatga
     4561 aggaagtaaa aaagaagaga atccaatttt acttgtagga aaagcagtaa catttgattc
     4621 tggtggaata tcgattaaac caagtgagaa aatggaggaa atgaagtttg ataaatgcgg
     4681 tggtgttaac gttattgcaa tcatgaaatc attatccgct ttaaatataa aacataacgt
     4741 gattggaata attcctgcag ttgaaaatat gccttctaat tcttcttata gacctagtga
     4801 cattatcaag atgtataatg gaaaaagtgt ggaggtgatt aacacagatg cagaaggtag
     4861 attaatactt gcagacgcat tagcatttgg aattaagcaa tataaaccca aactaataat
     4921 tgatatggcc acattgactg gagcttgtat tatagcatta ggtagtaata tagcaggtat
     4981 aataggtaac gatgaaaaac tgaccaaaaa agttcttgac atatctaaag ttaccgatga
     5041 aaaaatgtgg gaattgccat tattagagga tcatcatgaa cagataaaaa gtaaaaatgc
     5101 agatattaaa aatatcggag ga
//
LOCUS       DATAFW010000059         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3724467, whole genome shotgun sequence.
ACCESSION   DATAFW010000059 DATAFW010000000
VERSION     DATAFW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724467"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VHJ57_01395"
     CDS             complement(<1..656)
                     /locus_tag="VHJ57_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487221.1"
                     /translation="MMKNNGSFLLILFLILPLLVISISQFVYAQTVENEIIENTLEIE
                     KSTGKNALEIDKMIPSNLKITESAANNTTPQEFQNEDIVTDMGNATKGTESAIAGLVE
                     ESATGTESALTNATAYAREKIEEARSDRESAITETPETTTPAQPADTQNKRVLTLFEA
                     DEFNKMKKTAPPAQPETAQETAPPAQPETAQETAPPAQPETAQETAPPAQPETAQETA
                     P"
     gene            974..1897
                     /locus_tag="VHJ57_01400"
     CDS             974..1897
                     /locus_tag="VHJ57_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX2487222.1"
                     /translation="MINPNLKIGHVHLKVSNLEKSLQFYRSILGFKIVSQKEKTVFLS
                     SSINNDSSISLVVLTEIKNIATPYKFQHKPRIAGLYHFAILLPERRYLAYFLRHIQKN
                     LDSEYYEGMADHAVSESIYLHDPDLNGIEVYRDRDPTEWKWKENTVHMITEPLKVQDL
                     LKESSDVNNEWNELPPGTSIGHVHLHVSNLAKAKRFYKDILELNHTATYPGAYFFAAN
                     RYHHHVATNIWLGENIPPAYNDGNNKPGLDHYAIKLHNKNDLFLLKDRLLEFKIDVDE
                     KMAEKEDNQFFSSFYAYDFDGIKIQFLYNNH"
     gene            1956..2494
                     /locus_tag="VHJ57_01405"
                     /pseudo
     CDS             1956..2494
                     /locus_tag="VHJ57_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(2590..3678)
                     /locus_tag="VHJ57_01410"
     CDS             complement(2590..3678)
                     /locus_tag="VHJ57_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2487223.1"
                     /translation="MDQFVIIPSSDENQSEIIYFEPRIVKILSGMSITWMNRDTKTHR
                     LTSGNVDSLLPTEFFHTDDIVGGKSTTVRIESNQPAIPYYCSLHPAERGLIAILSKDE
                     DEMTNTQRLKILDSIAPTLFDTENREILTRLQRQVDPAVVEYLSNPNAVLLQNKVLTI
                     VFWDISGFSILCEKLIDHPELVAEFLREYLGVATKIIHEHMGIVDKFIGDGILAYFGF
                     KEKDYDNNGYTGAENAILAALKLKRPFENVKNNWLQIWNKVMDFDIHIDIKCGINTGP
                     VLVGLLRSGERDQFTVIGTHVNLASRLEGRAEEGEIIISTFTMAKVRGKFNVEAIDIS
                     NNDDIKIKSFKGVDKYYKVLDISSSNSS"
BASE COUNT         1335 a          583 c          594 g         1406 t
ORIGIN      
        1 ggtgctgtct cttgagcagt ttcaggttga gcaggaggtg ctgtctcttg agcagtttca
       61 ggttgagcag gaggtgctgt ctcttgagca gtttcaggtt gagcaggagg tgctgtctct
      121 tgagcagttt caggttgagc aggaggtgct gtctttttca ttttattgaa ttcatcagct
      181 tcaaacaacg taagcactct tttattttga gtgtctgcag gttgagcagg agttgttgtt
      241 tctggtgttt cagttattgc actttctcta tcagatcttg cttcttctat cttttctctt
      301 gcatatgctg tagcattagt tagggcactt tctgtaccag tggctgattc ttcaactaaa
      361 ccagctattg cactttctgt accttttgta gcattaccca tatcggttac aatatcttca
      421 ttttgaaatt cttgtggagt ggtattatta gcggctgatt ctgttatttt taaattgcta
      481 ggtatcatct tatctatctc caaagcattt ttgccagtag acttttctat ttctaaagta
      541 ttttctataa tttcattctc tactgtctga gcatatacaa attgagaaat tgatattact
      601 aataggggaa ggatcaaaaa tagaattaaa agaaaagatc cattgttttt catcatagca
      661 gttttgaata aataaatatt atttatctct ttccagatgc ttttataaat ttattaatct
      721 ataaaaatta attcttaata attaatatta gaaaatttta atttgctata tcaaaaagtt
      781 aaaataattg tttatatgat tttttgatat cttttctata tcaagagtta tattattatt
      841 atttgattct tcttgtccaa atattggata tgatgaaaag aatgtcttat tgtgatgtga
      901 tataacagag ctaaattaac cgtagtattc catcgcttta tcttaaatac ctaaaactat
      961 tattatccta atattgatta atcccaatct aaagataggc catgtacatc ttaaggtatc
     1021 aaatttagaa aaatctcttc agttttatag atctattctt ggcttcaaga tagtaagtca
     1081 aaaagaaaag actgtatttc tttcatcatc aattaataat gattcatcta tttctttagt
     1141 tgtattaact gaaataaaaa atattgctac tccttataaa tttcaacata aaccaagaat
     1201 agcaggacta tatcattttg ctattttatt accagaaaga agatatttag catattttct
     1261 tagacatata caaaaaaatc ttgattctga atattatgag ggtatggcag atcatgcagt
     1321 atcagaatca atatatctac atgatcctga tttgaatgga attgaagttt atagggatag
     1381 agatcctaca gaatggaaat ggaaagaaaa tacagtacat atgattacag agccactaaa
     1441 ggtacaagat ttattgaaag aatctagtga tgtaaataat gagtggaatg agcttcctcc
     1501 aggtacatca ataggtcatg tccatttgca tgtatcaaat cttgccaaag cgaaaagatt
     1561 ctataaagat attttggaac taaatcatac ggctacttat cctggagcat atttctttgc
     1621 tgcaaataga tatcatcacc atgttgctac caatatatgg cttggagaaa atattcctcc
     1681 tgcatataat gatggtaata ataagccagg attagatcat tatgcaataa aattacataa
     1741 taaaaacgat ttatttttgt taaaggatcg tcttttagaa tttaaaatag atgttgatga
     1801 aaaaatggct gaaaaggagg ataatcaatt cttttcatca ttttatgcat atgattttga
     1861 tggtataaaa atacaatttt tgtataataa tcactaaaaa tatgatagtt taaagtaata
     1921 ctatgacact ctaaaggtat gatactaagt taaaaattct tggttttgca ggcagtctca
     1981 gaacagcttc ttataataac tctcttttac gtgcagcagc aaatttaatg ccagaagata
     2041 caaatctaga gatatttgat attgatggca ttcctgcatt taatcaagat actgagaata
     2101 acatgtcaga gaaggtaaaa agactttaaa taaaaaaaag agaagcagat gcaattctaa
     2161 ttgcaactcc agaatataat tattcggtac caggtgtact aaaaatgcaa ttgattcggc
     2221 cacacggcca tatggagata atccatttaa tgaaaaacca gtagctataa tgagtgcatc
     2281 tgttggtatg ttgggtggag caagagcaaa gtatcatctt agacagatat ttgtgtatct
     2341 aaatatgtat cctattaacg gaccagaagt catagttaca tttgcacaag ataaatttga
     2401 tgcaaatgga aatttagtag atgaaaatac tcaaatgttt gtaagacaat tacttcaaaa
     2461 tcttgtaaat tggactaaaa aattgaaaca atagaataat tttaggtacg ataaataaag
     2521 tattagcatt tattctccat ggtattttta tagaaaaata acatacttca atttttaata
     2581 gtagaaattt taagaagagt tagaggaaga tatgtctaat actttataat atttatcaac
     2641 acctttaaaa gattttattt tgatatcatc attattagat atatcaatag cttcgacatt
     2701 aaattttcct ctaacttttg ccatcgtaaa tgtcgagata ataatttcgc cttcttcagc
     2761 tcttccttct aatctacttg ctaaatttac atgagttcct attactgtga attgatctct
     2821 ttctccactt cgcaagagtc caacaagaac tggtccagta ttaataccac attttatatc
     2881 aatatgtata tcaaaatcca tgactttatt ccatatttgt aaccaattat ttttgacatt
     2941 ttcaaatggt cttttcaatt ttagggcagc aagtattgca ttttctgctc ctgtgtatcc
     3001 attattatca taatcttttt ccttaaatcc aaaatatgca agtattccat ctccaataaa
     3061 tttatcaact attcccatat gttcatgtat tatttttgtt gcaacaccaa gatattctct
     3121 aagaaattca gcaactaatt ccggatgatc tattagtttt tcacataaaa ttgaaaaacc
     3181 actgatatcc caaaatacta tagtcaatac tttattctgt aacaaaactg cattaggatt
     3241 actaagatat tcgactactg ctggatcgac ctgtctctgg agacgagtta gtatttctcg
     3301 attctcagta tcaaaaagtg ttggagcaat tgagtccaaa atttttaatc tttgagtatt
     3361 ggtcatttca tcttcgtcct tggaaagaat tgcaattaat cctctttcag ccggatgaag
     3421 agaacaatag taaggaattg ctggttgatt gctttcaatt ctgactgttg ttgattttcc
     3481 cccaacaata tcatcagtat ggaaaaactc agttggcaat aacgagtcaa cattaccaga
     3541 tgttaatcta tgagtttttg tatctctatt catccaagtt atagacatac cacttaggat
     3601 tttgacaatt cttggttcaa agtatataat ttcagattga ttttcatctg aactaggaat
     3661 aattacaaat tgatccaatg taacaaatac aacaagtaat agatataatt agtttatatc
     3721 gtattgaaaa tagtgtaatg aatataccat taagaaatgg ttttttactc tttccatcct
     3781 ataatgagag gtaaactatt caaaatggct aaggtataat attaaacaac tcctgaatta
     3841 atcattatct tttctatcaa taaaaattta aattgaataa ttaatgaaac tcatttgtag
     3901 ctacatcaat aaattttg
//
LOCUS       DATAFW010000060         8688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6191395, whole genome shotgun sequence.
ACCESSION   DATAFW010000060 DATAFW010000000
VERSION     DATAFW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6191395"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VHJ57_01415"
     CDS             <1..253
                     /locus_tag="VHJ57_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX2487224.1"
                     /translation="VKVNEKWKQNKKSIMPQIDYGKCVFCGLCVDACPFYALYMTDDF
                     ELSSFTKEHLIYTPAQLAVKPKYDGTVEVKIDERGASHG"
     gene            228..842
                     /locus_tag="VHJ57_01420"
     CDS             228..842
                     /locus_tag="VHJ57_01420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX2487225.1"
                     /translation="MKEELLMADVAFIGLSILTVGSAIFALESREIIYGAIALAISML
                     GIAGFFILLDSPFVAMFQISVYVGAVAVLVIFIVMLVRTESLFSGDEDNRGRQIAGLI
                     FSLLAMAGMGVLLFSSLFSNAELKPSLENIVNKIGNGMLTYYSPVFILLGLTLAGSII
                     GALSLARREESDQPRSETSPASPQEEKIYLDKKSEGKGAGISND"
     gene            835..1137
                     /gene="nuoK"
                     /locus_tag="VHJ57_01425"
     CDS             835..1137
                     /gene="nuoK"
                     /locus_tag="VHJ57_01425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX2487226.1"
                     /translation="MTELINFVSISLILVGIGMYGLIVKRNAIRMIFAIEIIANAANL
                     NLIAFSRYLGDIQGQIFALFAIAIAAAEVAVGLAIIIIAYRLYKNIDVSEYKYLRG"
     gene            1190..3010
                     /locus_tag="VHJ57_01430"
     CDS             1190..3010
                     /locus_tag="VHJ57_01430"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX2487227.1"
                     /translation="MASESGILMASIFLPLVLAPLSYFLGKKRGTSFVTWFSFGILVI
                     STILLIIPAISISVDNPVYEESYAWSQFGNFGLKLDGFSIPFAVTIYVLSAVIAVFSK
                     EYMVRKIAGHFDKLEMSNPQYLNKDNSNNSTEEKLNKNNDLSLGGRGQIESVYSIEPS
                     YKQHIDTQMGLYFALFLTFSMGMVGTVLSTNLIEFYVFFELMLVPAFFLIALFGYSTR
                     KRVSLMFFFWTHVGAVLLLLGVLAMGLFAGGFDFETIKSNTSKIPSQWMTLIVFAVVI
                     GIAVKLGAFILHIWIPATYAESPTPISALIAGPMSAIGVYALLRIWIDLLAITYSDYS
                     IYINFWGVITMIYGGAMALMQDDVKKLLAYSSISHIGYIIFGLGSQSMLGITGSVLMW
                     IPHALGETILFLMAGSIILRTGTRSMSNLGGIARKMPYTATFAMIGVLTLIGVPPTSG
                     FMAEWIMFTGALQTGTENMDSFRVVLFSLAILSTILTSSYLLVMYKKIFFGKISPRFE
                     KLRDVNRYAIIPMAFIAGLSLFIGVYPDPVINPIIAYSQAIFSDSPEIASIPTVNNLN
                     TNIIGSVVILQNEYASIIDEDNRNFQQLRDVKSIEIKEDG"
     gene            3022..5166
                     /locus_tag="VHJ57_01435"
     CDS             3022..5166
                     /locus_tag="VHJ57_01435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX2487228.1"
                     /translation="MVESIGFEGIAINVWLVWILPFVGAALIPALAKKNIRITNIGAV
                     GFAIISALLAATLIPLAISGSEIHSQIQWISALNIKAGILADPLSIIMTNLVAWVSSL
                     IMIYSVGYMHGDRDLTRYWFFMLFFIGSMQLIVLSDNLLMVFFGWEGVGFASYALIGF
                     WYGDRKKDYVGREGHIVKGIAMWVSPTHAGIKAFLMTRAGDVMMLSGMFLIFMYAGTF
                     GFKELLADQTWAHTMMAQNLLVPAAVLLFGGAIGKSAQFPLNEWLIEAMTGPTPVSAL
                     IHAATMVKAGVFLVARIGPLFFALAAFDAAAFFEVVAWIGAITAFLLATQAIVNPEIK
                     KVLAYSTGSQIGYMMMALGIAGLSTQFVDGYTAGFFHLMSHAMFKASLFMAAGAILHI
                     VGSRFMTDMGGLRKNMNKTFIFMLLSALSLAGAPLITSGFWSKDAIFAALLQSGTSTG
                     GALFFIAFIVAIMTAFYTFRMVGMVFFGTKSKHIQTIEQTSYNNSQSVSSLSSQPSDE
                     HNHQQDTKHPIHEVSPIMWIPFAILAISTVFIGLVGFFFEQEIHSIMASYLSSSFGIG
                     EANSAHSTNNLGQSAGQETEALNPIALVSSFVAFGIGAGLGILFYIIRKKDPEIISRY
                     PVLKNIWLFLYNRWYINSLLYWGFVKIPLRLYQLIWLYFENMIAQGINPAIEYSMSFL
                     SKWVKYTQTGVTQTYLFVFAAGIIIVLIMLYL"
     gene            5187..6731
                     /locus_tag="VHJ57_01440"
     CDS             5187..6731
                     /locus_tag="VHJ57_01440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX2487229.1"
                     /translation="MIDISSTPIMIIILLGSVGLAIPIVDAYKKEKGKNKNKLFSAIT
                     FGTLILSLLIIISRVIGGEPLPGMNFGNDVLTDDMFGSFFAVSLLIVSIMVSASSWNF
                     MRGISNYSSYYSLLLLSTIGMILIAYSTDFVMLFIAWELMSIPTYALAAFSKKDPISN
                     EAAIKYFLFGAFSSSILVFAIGFVYAVTGTTNIGDSITLLAELSAANQTPDKLGGVIP
                     ISYLAIGLFVAGFGFKMGLVPFHMWLPDAYEGAPTTIGALLAAGTKKAGFAAAIRVIV
                     IGMFAIGLDWSIVLAILAVITMTVGNFGALIQKSVPRMMAYSSVAQAGYIMIGLALAP
                     YASSAMDGSLFHILNHAVMKSASFLAIAAVAIALASYSLEKYTGLGRKMPITAIALSI
                     SLLALAGVPPLNGFWSKLVLFQSAIDSGTVISWGPYLAIAGLLNSALSLGYYLWIIKK
                     MYLDESSDMSRVKEPKAILGVLVFAIIFMVGFGIWHAPLLEFASISVPTHIDQIVPPF
                     GNITQN"
     gene            complement(7248..7667)
                     /locus_tag="VHJ57_01445"
     CDS             complement(7248..7667)
                     /locus_tag="VHJ57_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX2487230.1"
                     /translation="MRIASFIPATEVRTYLSDRSYGGGYSGGGRRSGGGYGDRNRPRR
                     FRHSPVKVGEQYDVTIEAISNRGDSGVAKIEGFVIFVPGTKVDEKIKVKITKVGNGYA
                     TAELSQQTSDQTASENSEQLQEGTKEGEEEGEEEEKS"
     gene            7863..8282
                     /locus_tag="VHJ57_01450"
     CDS             7863..8282
                     /locus_tag="VHJ57_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2487231.1"
                     /translation="MIIIKIKYYKINKILSRVMGDMLHKICGINMIKDNSMKVFHLRP
                     DSKVINILIGKINGKLFACDNYCPHRGASLSKSAISYSKQRIVCYLHEFEYNLNTGKL
                     ENIPPKWLNQSPEWKKSNDLVIYKIVEKENNIYVDIP"
     gene            complement(8300..8617)
                     /locus_tag="VHJ57_01455"
     CDS             complement(8300..8617)
                     /locus_tag="VHJ57_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487232.1"
                     /translation="MLAKIIDYNKYMVRVTQLDIDDYGSIIPVGDVELLFSPNNLNVY
                     ELLKNSTYAAIFTEKDIYNEGSVDLKLDVNLNNIILVIPMTLDELNDEKKKVIESAGR
                     YKE"
BASE COUNT         2661 a         1384 c         1565 g         3078 t
ORIGIN      
        1 ggtaaaagtt aatgaaaaat ggaaacaaaa taaaaagagt ataatgccac aaattgatta
       61 tggaaaatgc gtattctgtg gactttgtgt tgatgcatgt ccattctatg cattgtatat
      121 gactgatgat tttgaattat cttcatttac caaagaacat ttgatatata caccagcaca
      181 attagcagtc aaaccaaagt atgatgggac agtcgaagta aagattgatg aaagaggagc
      241 ttctcatggc tgatgttgct ttcataggtt tatctatttt aacagtagga tctgcaatat
      301 ttgctttaga aagtcgagaa attatttatg gtgcaattgc tttggcaatt tcaatgcttg
      361 gtatagcagg tttttttatt ttactagatt ctccttttgt tgcgatgttt cagatctcag
      421 tgtatgttgg cgcagttgct gttttggtta tatttattgt aatgctagtc agaactgaat
      481 ctctcttttc tggtgatgag gataataggg gcaggcaaat cgctggttta atattttcat
      541 tattagcgat ggcagggatg ggagttcttc ttttcagttc gctgttttca aacgctgagt
      601 taaaaccatc actagaaaat atagtaaata aaattggtaa tggaatgcta acctattact
      661 ctcctgtatt tatattacta ggtctaacac tagcaggatc tattatagga gccttatcat
      721 tggcaagaag ggaggagagt gatcagccgc gctcagagac ttctcctgca tctccccaag
      781 aagaaaaaat atatcttgat aaaaaaagtg aagggaaagg agcaggtata agtaatgact
      841 gaattaatta attttgtatc aatttcattg attctcgttg gaataggtat gtatggtctt
      901 attgtaaagc gaaatgctat aagaatgatt tttgcaatag aaattattgc aaatgctgca
      961 aatctaaatt taattgcctt ttcacgatat ttaggagata tacaaggcca gatatttgcc
     1021 ctatttgcca tagcaatcgc agcagcagaa gtggctgttg gcttagctat aataattatt
     1081 gcttatagac tttataaaaa tattgacgtt tcagaataca aatatctacg gggttaacaa
     1141 tatgggatta tttaatgagt ttttagacat cgaggtagaa aaaaggtaga tggcttctga
     1201 atctggaata ttaatggcat ctatatttct acctttagta ctcgctccat tatcttattt
     1261 tttaggaaaa aaaagaggaa ctagtttcgt tacttggttt tcctttggta ttttggttat
     1321 atccacaatt ttattgataa tcccagcaat ttctatatca gtagacaatc ctgtttatga
     1381 agaatcgtat gcctggagcc agtttggtaa ttttggattg aaactggatg gtttcagcat
     1441 tccatttgct gtaaccatat acgtcttatc agcagtaata gccgtatttt caaaagaata
     1501 tatggttaga aaaattgctg gacattttga taaactcgag atgtcgaatc ctcaatacct
     1561 caataaagat aatagtaata attctactga agaaaaatta aataaaaata atgatttatc
     1621 actaggtggt aggggacaaa ttgaaagcgt ttacagcata gagccgtcat ataaacaaca
     1681 tatagatact caaatgggac tttattttgc cctttttctc acattctcta tggggatggt
     1741 aggaacagtt ctatcaacaa atctgatcga attttatgtt ttttttgaat taatgcttgt
     1801 tcctgctttc tttttaatag ctctctttgg atattctacg agaaagagag tatctcttat
     1861 gttctttttt tggacacatg taggtgcagt tcttttatta ttgggtgtgc ttgcaatggg
     1921 tttattcgca ggaggatttg attttgaaac aatcaaatca aatacgtcaa aaattccttc
     1981 acaatggatg acacttattg tgtttgctgt tgttatagga attgcagtaa aattaggcgc
     2041 ttttattctc catatatgga tcccagctac ctatgcggaa tctcctacac ccatttccgc
     2101 cctaattgct gggcctatga gtgcaatagg cgtttatgca ctattaagaa tttggataga
     2161 tctccttgct ataacctatt ctgattatag tatttacata aacttttggg gagtaataac
     2221 tatgatttat ggaggtgcca tggctttgat gcaagatgat gttaaaaagt tattggcata
     2281 ttcgagtatc agtcacattg gttacataat tttcggatta ggaagtcaaa gtatgttagg
     2341 tataacaggt agtgtattaa tgtggattcc tcatgccctg ggtgagacaa ttcttttctt
     2401 gatggcaggt tctataatcc tccgaacagg cacaagaagt atgtcaaatc tgggaggcat
     2461 tgcaagaaaa atgccttata cagctacctt tgcaatgatc ggtgtactaa cactgatagg
     2521 tgtacctccc acaagtggct ttatggccga atggataatg tttactggtg ccctacaaac
     2581 tggaacagaa aatatggatt cttttagagt tgtcttgttt tcccttgcta ttctttctac
     2641 tattctgacc tcttcttacc tcttagtgat gtataaaaaa atattctttg gaaaaatttc
     2701 acctcgcttt gaaaaattac gagatgtaaa tagatatgca ataattccga tggcctttat
     2761 agcgggttta tcattgttta taggtgtgta tcctgatcct gttattaatc ctataattgc
     2821 atattcacaa gcaatttttt cagattctcc cgaaattgct tctattccaa cagttaataa
     2881 tttaaatact aacattatag gatccgtagt tattctacaa aacgaatatg caagcattat
     2941 agacgaagac aatcggaatt ttcagcagtt aagagatgtt aaaagtatag agattaagga
     3001 ggatgggtga aaagaaaaaa aatggttgaa tcaataggtt ttgaaggaat tgctataaat
     3061 gtctggcttg tttggatact gccatttgta ggtgcagcat taattcccgc tttagccaaa
     3121 aaaaacatta gaattacaaa tatcggtgct gttggatttg caattatcag tgcgcttctt
     3181 gccgctactc tcattccact agctatctct ggaagtgaaa tccatagtca gatacaatgg
     3241 atctctgcat taaatatcaa agccggaata ctggcagatc cactgagtat tattatgaca
     3301 aatcttgttg catgggtatc atcattaatt atgatttact ctgttggtta catgcatgga
     3361 gatagggatc taacacgata ctggttcttt atgttgtttt ttataggatc aatgcaattg
     3421 attgttctct cggataatct cttgatggta ttttttggat gggaaggcgt aggctttgca
     3481 tcatatgcat tgataggatt ttggtatgga gatagaaaaa aagattatgt tggcagagaa
     3541 ggtcatattg taaaaggtat agcaatgtgg gtatccccta cgcatgcagg aataaaggca
     3601 ttcttgatga cacgcgcagg agatgttatg atgctttctg gcatgttttt aatttttatg
     3661 tatgcgggga cttttggttt taaggaactg ctagctgatc aaacttgggc tcatactatg
     3721 atggcacaaa accttttggt accagcagct gttcttcttt ttggaggagc gataggaaag
     3781 tcagctcaat ttcctttaaa tgaatggtta atagaagcga tgactggccc tacacctgtt
     3841 tctgcattga tacatgcggc tacaatggtt aaagctggtg tttttcttgt agctaggatt
     3901 ggaccattat tttttgcctt ggccgcattt gatgctgcag ctttcttcga ggtagttgca
     3961 tggataggtg caataactgc atttctgctt gcgacccaag ccattgtgaa tcctgaaatc
     4021 aaaaaggttt tggcatattc cacaggctca caaattggtt atatgatgat ggcactagga
     4081 atagcaggcc tttctactca attcgttgat ggttatactg ctggattttt tcatctaatg
     4141 agtcatgcga tgtttaaagc atccttgttt atggcagctg gtgcgatatt acatatagtg
     4201 ggttctagat tcatgacgga tatgggagga ctacggaaaa atatgaataa aacgttcatt
     4261 tttatgttac tttcagctct ctcattagca ggtgctcctt taataacttc tggattttgg
     4321 agtaaggatg cgatttttgc tgcactctta caatcaggaa catctactgg aggagcgctt
     4381 ttcttcattg catttatagt tgctataatg actgcatttt atacatttag gatggtagga
     4441 atggtctttt tcggtactaa gagtaaacat atacaaacca tcgaacaaac ctcttataac
     4501 aattctcaat ctgtgtcttc tttatcttct caaccttcag atgaacataa ccatcaacaa
     4561 gatacaaaac atccgattca cgaggtttca ccaataatgt ggattccatt tgctatctta
     4621 gcaatttcta ctgtatttat tggattagtt ggatttttct ttgaacaaga gatccattcc
     4681 atcatggcat cttatctttc cagctcattc ggtattggag aagcaaacag cgctcattct
     4741 acaaacaatt tagggcagag tgcaggccaa gaaacagaag cattaaaccc aattgcatta
     4801 gtgtcatcat ttgtagcttt tggtataggg gctggattag gaatattatt ttatattatt
     4861 cgaaaaaaag atccagaaat tatttcacgc taccctgttt taaagaatat atggcttttc
     4921 ttgtataata gatggtacat taatagttta ttgtattggg gatttgtaaa aataccgctc
     4981 cgtctatacc agctcatttg gttatatttt gaaaatatga ttgcacaagg aattaatcct
     5041 gctatagagt attctatgag ctttttgtca aaatgggtaa aatacactca gactggagta
     5101 actcaaacat atctttttgt cttcgcggct ggaattataa tcgttctaat tatgttatac
     5161 ttataggaga aatgaagaga taagatatga ttgatatttc gtctacgcca attatgataa
     5221 ttatacttct tggctcagta ggtcttgcaa tacctatagt agatgcctat aaaaaagaaa
     5281 aaggaaaaaa taaaaataaa ctgtttagtg ccataacttt tgggacttta attctttcct
     5341 tacttataat aatctctcga gtgataggtg gagaacccct gcctgggatg aattttggaa
     5401 atgatgtgct gacagatgat atgttcggtt ccttctttgc ggtatctctt ttaatcgtct
     5461 ctataatggt ctcagcttct tcatggaact ttatgagagg aatttccaac tattcttcat
     5521 actattcgtt acttttacta tcaacaatag gaatgatatt gatagcctat tcaaccgatt
     5581 ttgtcatgtt gtttattgct tgggaactta tgtcgatacc aacatacgct cttgctgcat
     5641 tttccaagaa agatccaatc tcaaatgaag ctgctataaa atattttctg tttggggcat
     5701 tctcatcctc cattctagtc tttgccatag gatttgtcta tgcagttaca ggcactacaa
     5761 atattggtga ttctatcaca cttcttgctg agctttcagc agcaaatcag acgccagaca
     5821 aactaggagg tgttattcct atttcttatc ttgcaatagg tttgtttgtt gctggctttg
     5881 gatttaaaat gggattggtt ccatttcata tgtggttgcc tgacgcatat gaaggtgcac
     5941 ccacaacaat aggtgcctta ttggccgcag gaacaaaaaa agcaggattt gcagcagcca
     6001 ttagagttat cgtaattgga atgttcgcaa ttggacttga ttggagtatt gttcttgcta
     6061 ttttagcagt aattactatg acggtaggta attttggtgc tcttatacaa aaaagcgttc
     6121 ctcgaatgat ggcttattct agtgtagctc aggcagggta tataatgatt ggactagcac
     6181 tagcccccta cgcaagttct gctatggatg gttcactttt tcacattctt aatcatgctg
     6241 taatgaaatc ggcctcattt ttagctattg ctgctgttgc aatagcatta gccagttaca
     6301 gtttagaaaa atacactgga cttggaagaa aaatgccaat cacagcaatt gcattatcaa
     6361 tatcattatt agcattagct ggggttcctc cattgaatgg tttttggagt aagttggtac
     6421 tttttcaatc tgcgattgat agtggaaccg taatatcctg gggcccttac ttagcgattg
     6481 ctggattatt aaatagtgcc ttgtctctcg gttattattt atggattatt aaaaaaatgt
     6541 acctcgatga aagtagtgat atgtcaaggg taaaagaacc taaggccatt ctaggtgtgt
     6601 tagtatttgc tatcattttt atggttggat ttggtatatg gcatgcgcca ttactggaat
     6661 tcgcctcaat atctgtccct acgcatattg atcaaatagt tcctccattt ggaaatataa
     6721 ctcaaaatta aatattctct tgttttgatt ttcaaatcta atagataaac aatttttatc
     6781 aggtcaataa atacctggct tggaatatca ataataatat ttagaagatt ctctacaaag
     6841 ttattattaa atgtatatta ttcacagttg gtagtttagt ttagagagaa caaatgaaca
     6901 ttttatatat gaaagaaaca ttccaagtgc ttattaatca tattttttgt ctttatttag
     6961 gaatttaatt ttagtgatca tatacatgtt tataatatta ttcaatagag gaagatagaa
     7021 agattaaatt atttaaccaa ctaataacaa tttaaaaata cattacatga tattcaataa
     7081 ctacaattac atcatgttaa agcttatttt ttattatttt gatcttaata ttttacataa
     7141 attgtaaaca ttatcaaaaa taaaaatcta gtttagagat aaatcggcga ttgctttaaa
     7201 aataaaagct atcctgtcca caattctcgg atttttaaaa aatcttgtta tgacttttct
     7261 tcttcttctc cttcttcttc tccctcttta gtaccttctt gtagttgttc agagttttcg
     7321 gaagcagttt gatcgcttgt ttgttgagat aattctgcag tagcatagcc atttccaact
     7381 ttagttattt tcactttaat cttttcgtca accttagttc caggaacaaa tattacaaat
     7441 ccttctatct tagcaactcc cgaatctcct ctgttactaa tagcttctat tgttacatcg
     7501 tattgttcgc ctaccttaac tggtgaatga cgaaaacgtc taggtctatt tctatctcca
     7561 tatcctccac cacttcttct tcctccacca ctatatcctc caccatagga cctatctgaa
     7621 agatatgttc taacctcagt tgctggtata aaactagcta ttctcaatgt tttcatagat
     7681 tctatatttt cttagatatt tcaactttag tatttttaac ttaaacaaaa atgatcttat
     7741 ggctatgttt agttgatttc aataaaaaag gtaatatctt gaataataac aattctatgt
     7801 tttagttgta ctataactcc caacgtcatc gtctctctaa attttaaaaa tatttagaag
     7861 aattgattat tataaaaata aaatactata aaataaataa gatactttct cgagtaatgg
     7921 gagacatgct tcacaagata tgtggtatca atatgataaa agataatagt atgaaagtct
     7981 ttcatttacg acctgatagt aaagtaatta atattttaat cggtaaaatt aacggtaaat
     8041 tatttgcctg tgataattat tgtccacatc gaggtgcctc tttatccaag agtgctatat
     8101 catatagcaa gcagcgaata gtatgctatt tgcatgaatt tgagtataat cttaatacag
     8161 gaaaacttga aaatattcct cccaaatggt tgaatcaaag tccagaatgg aaaaaatcaa
     8221 atgacttagt tatttataaa atagttgaga aagaaaataa tatctatgta gatattcctt
     8281 aagattttat ttatttattt tattctttgt atctcccagc tgactcaatt actttctttt
     8341 tttcatcatt aagttcgtct agggtcattg gaattaccaa aatgatatta tttaagttga
     8401 catctaattt caagtctacc gatccttcat tataaatgtc tttttctgta aatattgctg
     8461 catacgttga attttttaat aattcataaa cattgagatt atttggggag aataataatt
     8521 caacatctcc aactggtatt atagagccat aatcatcaat atccagttga gtaaccctta
     8581 ccatatattt attatagtca ataatctttg caagcatatt aatatataat atatataata
     8641 aacgtaaatt aataactttt cttagacaaa taattaatta ttttgttg
//
LOCUS       DATAFW010000061         7271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_553637, whole genome shotgun sequence.
ACCESSION   DATAFW010000061 DATAFW010000000
VERSION     DATAFW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553637"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1636)
                     /locus_tag="VHJ57_01460"
     CDS             complement(233..1636)
                     /locus_tag="VHJ57_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX2487233.1"
                     /translation="MTNSPELITINPTTEKILKKYTIMTKENVSSIVKKARDIYKEWE
                     KDLDKRIDSLYHVAQELRKNREDLARTATNEMGKAIKEARAEIDKCAWAIEYFADNGK
                     NFIHDESFNTDARKSIIKFEALGVIGSIMPWNFPYWQALRFAAPSLIVGNTIVLKPAS
                     ATMQCGIEIEKTFDKAGLSEGIFNTVIGDSSVAEILVDSDVNAVTFTGSVPVGAKVAQ
                     RATSQLKKTVLELGGSDPFIVCADADIEKASTGAVKGRFINCGQSCIASKRFIVIKNI
                     AKEFIEKFIQKTENLKVGDPSLDDTDIGPLVNAKALQTIDSQVEDSIKEGAEVLTGGE
                     QIVGSKGYFYKPTVLGNITPTMRIVNEEVFGPVAPILIADNEKEAIKLANGSEFGLGA
                     SIWTEDLENAELLSSNVKSGIVAVNNVVVSDPRVPFGGIKKSGFGRELSRYGMLEFVN
                     IKSIRLYDQLIHEHYVE"
     gene            complement(1633..2688)
                     /locus_tag="VHJ57_01465"
     CDS             complement(1633..2688)
                     /locus_tag="VHJ57_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX2487234.1"
                     /translation="MKSAKIIEIKRPLQIQENKTPKPKGSQVLVKVQSSGVCHSDIHL
                     WEGGYEGPQGQFLKTTDRGVKYPLTPGHEIAGVIDSLGDQTEGFAKNEKVLVFPWIGE
                     GLCPACRTGEENLCDKPRSLGIYTDGGYADYVLVPSYKYLVKLGDEIDTDTSAPLSCS
                     ALTAYGAVKNGNLKPNDNVVIVGTGGLGLMAIQLAKAITGSRIIALDRDDKKLKAAKE
                     NGADEIINSKKEDAVKAVMELTGRMGADAVIDFVNASSTVETDMNFLRRRAKVVLVGL
                     FGGELKLNLISMPTRSYKLIGSYTGSINDLIELVSLAKRDIIKPIISNKFKLEEATKA
                     LKMLNDGKILARGVINP"
     gene            complement(3084..4589)
                     /locus_tag="VHJ57_01470"
     CDS             complement(3084..4589)
                     /locus_tag="VHJ57_01470"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX2487235.1"
                     /translation="MPFKESHSIDDSLKITHGLASGDVTKNSTIIWSKSNTQSIMNVS
                     YNKSNDLLNNFSSIVLKTLVNSSTDFAGHVKLINLKSNTTYYYKVWFADLNNSSLKSD
                     YKTGKFTTSPNENELTQIDFVIAGDLGGGTLCRQQEIGYSIFSIMKLTAPDFFIFNGD
                     QIYADGTCNDLTDLVKNNYTYWQNIEGNFKRVNDIDWNDQDELNKTFLQHWEYNRYDS
                     HIQNFLNDTSMYSQLDDHEVIDNYGNWSFYEVSKDPNERNKSGYPNLVKTGVKYFFEF
                     SPIEKNKERPDQIYRMFQWGKNLDLFLLDTHSYRSINTLQDTIQNNKTLYGTQQLDWL
                     KNKLLSSNATWKIISNPIPITIPDCIGYMQACNNWATNNASNTLTFTNERNQFLKFLD
                     ERNIKNVIFLTTDVHYASTVKVSQDFDRDGDIFTFYEMTNGPLSAYTSDKPYPLDPTI
                     NATYIYSESALFNFGHVKIEKEKDGKYHFIYDVIDSNNRIRPNSTINLIPK"
     gene            complement(4977..6128)
                     /locus_tag="VHJ57_01475"
     CDS             complement(4977..6128)
                     /locus_tag="VHJ57_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HEX2487236.1"
                     /translation="MSMNHKKDSSQNDTSIRELPAEEPLGVLEPVAYFYGAMPTGVSV
                     SHQGRIFVNFPKWGDDVQFTVAEMKNGKAVAYPDESFNQTDPDDPAKALVSVQSVVVD
                     PVDRLWILDTGSPFFQPTEYGGPKMICVDLTNNKVVKKILFPQDVALPTTYLNDVRFD
                     LRRGNEGMAFITDSSQNGANGIIVVDLASGESWRHLHDHPSTKAEDLQTFLPIVEGRP
                     FLQHQPDGTVKQGASMGTDGIAINADGSRLYYCPLGSRKLYSVDIDALADRSHETKKV
                     ADTVADEGDKGGASDGLESDATGYIYSTNYEHNSILRRNPDNRQWETIVHDPRLLWPD
                     TLSVALDGYLYVTANQLHRQARYQKGQDLRRKPYTLFRVHIGTSPVLLR"
     gene            complement(6493..6639)
                     /locus_tag="VHJ57_01480"
     CDS             complement(6493..6639)
                     /locus_tag="VHJ57_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487237.1"
                     /translation="MPRGIKKGVNTKKFDRCVEKVKGKSGKKVNPYAVCNASLSGKSK
                     KKKD"
     gene            7026..>7271
                     /locus_tag="VHJ57_01485"
     CDS             7026..>7271
                     /locus_tag="VHJ57_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX2487238.1"
                     /translation="MQLNNHCLGKEAELYIKLPNGRIKCTACARYCEIPEGKDGLCGI
                     RGVENGRLQLYAYGRIIAGHIDPIEKKPVTHYMPGSNI"
BASE COUNT         2289 a         1274 c         1036 g         2672 t
ORIGIN      
        1 ttcttacaat tgtattttca gctgctttac caatattttc aattgttgtt gttgatcttg
       61 tttttgttat atattgtgaa gccgtacatc aaatttgtat tctatatttt aagatagttt
      121 tttacttgca attattctat atagctagca ctaaagctta atcttaaaga tcattatcta
      181 tttattaaaa aataatgcat tagtatatct agtaatagca tctttaagat tatcattcta
      241 catagtgttc atgtattaac tggtcataaa gtctaattga tttaatattt acaaattcta
      301 gcataccata tcttgaaagt tctcttccaa atccactttt tttaataccg ccaaatggaa
      361 ccctaggatc tgaaactaca acattattga cagctactat acctgacttt acgttactag
      421 ataataattc tgcattttca aggtcttctg tccaaatact agctcccaga ccgaattcag
      481 agccatttgc aagttttata gcttcttttt cattgtcagc aataagaata ggagcaactg
      541 ggccaaaaac ttcctcattt actatacgca tagttggagt aatattgcca agaacagtcg
      601 gcttgtaaaa ataaccttta cttcctacta tttgttctcc acccgtaagg acttccgctc
      661 cttccttaat cgagtcttca acttgtgaat ctattgtctg taatgccttt gcatttacaa
      721 gtggtcctat atcagtatca tccaaagaag gatcgccaac cttaaggttc tcagtttttt
      781 gaataaactt ttcaataaac tcctttgcaa tattctttat tacaataaat ctttttgacg
      841 caatacaact ttgaccacaa ttaataaaac gtccttttac tgctcctgtt gacgctttct
      901 ctatatccgc gtctgcacat acaataaaag gatcgcttcc acctagttca agtacagttt
      961 tctttagttg tgaggttgct ctctgtgcaa ctttagcacc gacaggaaca cttccagtaa
     1021 atgtaacagc atttacatct gaatctacaa gtatttctgc aacactagag tctcctatta
     1081 ctgtgttaaa aatgccttca gataatccag ctttatcaaa ggttttttca atttctattc
     1141 cacattgcat tgtagcacta gctggtttta atacaattgt atttcctact attaacgaag
     1201 gcgctgcaaa tcttaatgcc tgccaatatg gaaaattcca tggcattata cttcctataa
     1261 cacccaatgc ttcaaatttt ataatactct ttcttgcatc agtattgaaa gattcatcgt
     1321 gaataaagtt ctttccgtta tctgcaaaat attcaatagc ccatgcacat ttatcaatct
     1381 cagctcttgc ttcttttatt gctttaccca tttcatttgt agctgttcta gcaagatcct
     1441 ctctgttttt tcttaattct tgagcaacat gataaagaga atcaatccgt ttgtccaagt
     1501 ctttttccca ttctttatag atatctcggg ctttctttac aatcgaactt acattttctt
     1561 tggtcattat agtatatttt ttgagaattt tctctgtggt aggatttatg gttattaatt
     1621 caggagagtt tgtcaagggt ttattacacc tcgagcaaga attttaccat catttaacat
     1681 ttttaatgct tttgtagcct cctcaagttt gaatttatta gagataatcg gttttattat
     1741 gtctcttttt gccaatgaaa caagttctat taaatcattg atacttccag tataagatcc
     1801 aattaatttg tacgatctag tgggcattga aattagattc aattttaatt caccaccaaa
     1861 aagtccaact aataccactt ttgctcttct tctaagaaaa ttcatatcag tttctactgt
     1921 tgaggaagca ttaacaaaat caattactgc atcagctccc attctcccag ttaattccat
     1981 aactgctttc acagcatctt ccttttttga attgataatc tcgtctgcac cattttcctt
     2041 tgctgcttta agctttttat catctctatc taacgcaatt attcttgaac ctgttattgc
     2101 ctttgctaat tgtatggcca tcaatccaag tcctcccgtc ccaacaatta ctacattatc
     2161 attaggtttc agattaccat tctttacagc accatatgct gtaagtgcag aacatgataa
     2221 aggagcactt gtatcagtat ctatttcatc tccaagttta actaagtatt tgtaacttgg
     2281 tactaaaaca taatctgcat atcctccgtc agtataaatt cctaaggacc ttggtttatc
     2341 acaaagattt tcttctcctg ttctgcatgc aggacatagt ccttccccaa tccatggaaa
     2401 cacaagaact ttttcattct ttgcaaaccc ttctgtctgg tcgcctaggc tatctattac
     2461 cccagcaatt tcatgtcctg gcgttaaagg gtattttact cctctatcgg tagttttcaa
     2521 aaattgtcct tgcggacctt catatcctcc ctcccataag tgaatatcac tatggcaaac
     2581 tccagatgat tgaactttta ccaacacttg agagcctttg ggttttggag ttttattttc
     2641 ttgtatttga agaggtcgct taatctcaat aattttagca gatttcattt cttattttat
     2701 ccaccattaa caatttttta tttgacttca acaaaatatt agattaatac aaaatatatt
     2761 aagattatct atttaattct caattactat tattataaaa tatatactat tcttgtatct
     2821 ctattaaata taagaaatat ctaaataatt agataatttt ctaattttag ttacatccat
     2881 ttagacatct tttgataagt atttgattgt attattctcc tttagaattc ttgatgatat
     2941 atattcaaaa aatcagtatt gaaatagata tttcttgtta tataattcgt ttcctttttt
     3001 tcttttctcc ttagcgcttc taatacttaa ttctaatttt atatttaatt tttttataaa
     3061 actattatgt attgagatgt atcttattta ggaataagat ttatagtcga atttggtcgt
     3121 attctattat tagaatcaat aacatcataa ataaaatgat atttaccatc tttttctttt
     3181 tctattttaa cgtgaccaaa attaaaaagg gcactttcac tgtatatgta cgtggcatta
     3241 attgtaggat ctagaggata tggtttatca cttgtatatg cacttaatgg gccattagtc
     3301 atttcgtaaa atgtaaatat atctccatct ctatcaaagt cttgagatac ttttactgtt
     3361 gaagcataat gtacatcagt tgtaagaaag ataacatttt taatgtttct ttcatctaga
     3421 aactttagaa attgatttct ttcattagta aatgttagag tatttgaagc attattagtt
     3481 gcccaattat tgcaagcctg catataccca atacaatcag gaatagtaat agggatagga
     3541 ttagatatta ttttccaagt agcattagat gacaaaagct tgttttttaa ccaatcaagt
     3601 tgttgtgttc cgtataatgt tttattgttc tggatagtgt cttgtagtgt atttatacta
     3661 cgatatgaat gtgtatctaa taagaataaa tcaagatttt ttccccattg aaacattctg
     3721 tatatttggt ctggtctttc tttatttttt tcaatgggag aaaattcaaa gaagtattta
     3781 actccagtct taactaaatt aggataacca cttttgtttc tttcatttgg atctttagat
     3841 acctcataaa aagaccaatt tccataattg tcaatcactt catgatcatc taattgtgaa
     3901 tacattgatg tatcgttaag aaaattctgt atgtgtgaat catatctgtt gtattcccaa
     3961 tgttgcaaaa atgttttatt taattcatct tgatcattcc agtctatatc atttactcgt
     4021 ttaaaatttc cctctatatt ttgccaatat gtataattat ttttaactaa atctgttaaa
     4081 tcattacaag ttccatctgc ataaatttga tctccattaa aaataaaaaa atcaggtgct
     4141 gttaatttca ttattgaaaa aatagaatat ccaatttctt gttgtctgca taaagtccct
     4201 ccacctaaat cccctgcaat cacaaaatct atttgtgtta gctcgttttc attaggagat
     4261 gttgtaaatt taccagtttt ataatcagat ttcagtgatg aattattgag atctgcaaac
     4321 catactttgt aataataagt tgtattggat tttaaattaa tcaatttcac atgtcctgca
     4381 aaatctgttg aggaattaac caaagttttt aaaacaatac tagaaaaatt atttaaaaga
     4441 tcattggatt tattatatga cacgttcata atggattgtg tatttgattt tgaccagatt
     4501 atagtactat tttttgttac atctccacta gcaagaccgt gtgtaatctt taaactatca
     4561 tctatgctat gagattcttt aaatggcatt aaaaaaatag tagatataaa aaatgttata
     4621 ataactatag tcaacttact acgaaattta gtaaaattat gattatttag cattactatt
     4681 attatttttc aaacatcatg caaagtataa aaaatttttc taaaaataac ttttgacagg
     4741 gttatttgct tgaatcttat aatggagtgt aaaaagtcac atttattttt actaaaaggg
     4801 aatgttttac cgtatcaagc agttgactat tcaccaccct tagttcgctc actaaggtta
     4861 ttagttttag attattaaat ttataatttt tttatttact aaattaatgc atattaagaa
     4921 ttaggaatta ataattataa ttttctttat cttaatatag taatatacct atggtcttag
     4981 cgcaagagga ctggtgaagt gccaatatgg acccggaaca aagtgtaggg tttacggcga
     5041 aggtcctgtc ctttttggta tcgtgcttga cgatgtaact ggttggccgt aacatatagg
     5101 tatccatcca aggctaccga aagtgtatca ggccataaga gacgcgggtc atgcactatc
     5161 gtttcccatt gcctattatc aggattacga cgtagaatag aattgtgctc atagttggta
     5221 gaataaatat aacctgttgc atccgattct agtccatcag aagctcctcc cttatctccc
     5281 tcatcagcca ctgtatcggc gacttttttc gtttcatgag atcgatctgc tagcgcatcg
     5341 atgtccacac tgtaaagctt acgacttcca agcggacaat aatatagacg agaaccatca
     5401 gcgtttattg caatcccatc agtgcccatg ctagcaccct gctttactgt tccatccggc
     5461 tgatgctgca gaaaaggcct tccttcaaca atcggaagaa aggtttgcaa atcctctgct
     5521 ttagtcgagg gatggtcatg gaggtgacgc caactctcgc cggatgccaa gtccacaacg
     5581 ataattccat ttgctccatt ttgggatgag tcggtgataa acgccatacc ttcgttacca
     5641 cgtcttaagt caaaacgtac atcatttagg taagtcgtgg gcaaagccac gtcttgaggg
     5701 aagagaatct tcttgacgac cttattattg gttaaatcga cacagatcat cttaggacct
     5761 ccatattcag ttggttggaa aaagggacta ccggtatcaa gaatccatag acgatcaaca
     5821 ggatcgacaa ctactgattg tactgatacc agtgccttag caggatcatc tggatctgtt
     5881 tgattaaatg attcatcagg ataggcgacc gccttaccat tcttcatctc agctaccgta
     5941 aattgtacat catcacccca ctttggaaag ttgacgaaaa tacgtccctg gtgtgacaca
     6001 ctcacgccag ttggcatagc tccgtaaaag taggcgacag gttctaatac acctagaggt
     6061 tcctctgctg gcaattcccg aattgaagta tcattttgtg atgaatcttt tttatgattc
     6121 attgacatgg ttatttaacc ttctttttct atgatggccc cattaatttc gattttacaa
     6181 atattatgat tatatattaa ccgtgaaatc aataaagagt gaaaaacaaa gagtattaaa
     6241 tctagtagta gcatagtatg tcctatttct ttgtaataaa aaggattatt cttaatcatt
     6301 gtgttactaa gtataggtaa tatattatat aatggttatt tgttgtagtt acatcagaaa
     6361 tctcataatt agtttaatct aacataaatt acattaagaa attattaaaa ttatattttg
     6421 agcctttctg caataactga actaataaat atttttgaaa aatcaatttt ataacataat
     6481 taaactcttt aactagtctt ttttcttttt tgattttcca ctcaaactag cattacatac
     6541 tgcatatgga ttgactttct ttcctgattt tcctttcact ttttcaacac atctatcaaa
     6601 tttttttgtg tttactcctt tcttgatgcc tcttggcata ttatatctat atcaatatat
     6661 ttaattaaga ttatagaaaa aagagtaaaa aaatatttat aaaattactt caaaacaaag
     6721 gaatgataat agctaaatta gatttatttt tatcatgcat tgtttagcag tattaatata
     6781 aaatttgaaa atatctagat agccgaaata gaaactagaa cagtccacat ggttattctg
     6841 caaaggttca gaaacatcta aaaaaatgtc agaactttat tgacaaaaca agatttaaat
     6901 cctgatttaa aggattcatc tatttttaca ataaacacaa attcaagtaa gaatttcaaa
     6961 acatttcata actgttatta aaaatttaag tactcttata atgttgaaat gtaaatataa
     7021 tattattgca attaaataat cattgcttag gaaaagaagc agaattatac ataaaactac
     7081 caaatggtag aataaaatgt acagcttgtg cccgatactg tgagatacca gaaggcaaag
     7141 atggtctttg tggtattaga ggagtagaaa atggaaggct ccaattgtat gcatatggta
     7201 ggataattgc aggacatatt gatcccatag aaaagaagcc agttacacat tatatgcctg
     7261 gaagtaatat a
//
LOCUS       DATAFW010000062         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3902564, whole genome shotgun sequence.
ACCESSION   DATAFW010000062 DATAFW010000000
VERSION     DATAFW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3902564"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..>2057
                     /locus_tag="VHJ57_01490"
     CDS             466..>2057
                     /locus_tag="VHJ57_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX2487239.1"
                     /translation="MMDKGDFFSRKRKEIGIISVVAIIISSFSLFFYQQNITEQNIKD
                     SIFNQYKNRQIESTQIISERIESDLNLIMSILQGISDSVPLQEGVLYGDRIQKLMKEK
                     FDQINNITKVDGLFIADENNIITYNIVSEGKRSFVNIDISFTDYIQETRNTLKPVFSD
                     GFNGIDNIFSIALTVPIVNSDSGKYIGTIGIQIPTAPFFAHSGNINNVNSQFLVAYDS
                     KKNYLVTPRTQFIGKNFFDNDVQNFFNVTDIQNNYYRKVFSGKLLGGSAVYDFGSGER
                     LNTGYPILLQEKPKYFIFIITPTTQIYSHINDILHTERIKVISLIFGHSFAVLILILF
                     LIKWNYTLNTEVRKRTKQLRESNRQLSLSNQEIVQANEELKVHDKMQKEFINVASHEI
                     KTPLQAIISASQLLHMYPERQKKFASAIQRNAIRLQRLSNDILDVAKIESRSLKLKKE
                     LLDLNELISNIIEDYKTIIIKDNQNVKLYFKPSHDPLFVEADKERIAGVISNLLNNAI
                     KFTKEGEIFVSTEKEDNSNNPYA"
BASE COUNT          834 a          258 c          292 g          673 t
ORIGIN      
        1 atgcatacca aataattgaa ttattattac agatgattat aataaatata tactaaataa
       61 tccaagtcca ctcactcaaa caatactatg atgtttcaag tatggtataa ttaaacctaa
      121 acttgattgg cacaattctt atcaagaaaa gctacttcac attgtgatct ttttgtattg
      181 aaaagaaatg tatatgatca aacaaaaaaa ccaaccaatt attaacttta gtaaacaaac
      241 tttcaaaata taattagaga ctttgaggct ttatagcata agaaacctta taattaagca
      301 ctagaaaata aaatttgtta aaataaaaaa ttaaactcaa tcattttaga gaataaaaat
      361 tagaaaagac catgatacaa aaagatctga tatagttaat gtttctaaat ataaagagat
      421 ttaatacata attaatttgt attttattga taggaggaag gaataatgat ggataagggc
      481 gacttcttct ctagaaagag aaaagagatt ggtattattt cagtagtcgc aataattatt
      541 tcttcattta gtttattttt ttatcaacag aatataacag aacaaaatat taaggatagt
      601 atatttaacc agtataaaaa ccgtcagata gaatctacac aaattatttc agaacgtatt
      661 gaatctgatc tgaatttgat tatgtcaatc cttcagggaa tctcagattc tgttcctcta
      721 caagaaggtg tactctatgg agatagaatt caaaagctta tgaaagaaaa atttgatcaa
      781 attaataata ttaccaaagt tgatggactg tttattgctg atgaaaataa tataataaca
      841 tataatatag tatctgaagg aaaaagaagt tttgttaata ttgatatttc atttacagat
      901 tatatacaag agacaagaaa tactctaaaa ccagtttttt cagatggatt taatggaatt
      961 gataatatat ttagtattgc attaactgtt ccaatagtaa atagtgatag tggaaaatat
     1021 ataggaacaa ttggtataca aatacctact gcgccttttt ttgcacattc tggaaatatt
     1081 aacaatgtta attcacagtt tttagtagca tatgatagta aaaaaaatta tcttgttact
     1141 cctaggacac aattcattgg aaagaatttt tttgataatg atgttcaaaa tttttttaat
     1201 gtgacagata ttcaaaataa ttattaccgt aaagtttttt ctgggaaatt attaggagga
     1261 tctgcagtat atgattttgg aagtggtgag agactaaata cgggataccc aattttgttg
     1321 caagaaaaac ctaaatattt tatattcatc ataacaccta ctacgcaaat atattcacat
     1381 atcaatgata ttcttcatac ggaaagaata aaagttattt cgttgatttt tggccattca
     1441 tttgcagttc tgattttaat tctatttctt ataaagtgga actatacgtt aaatactgaa
     1501 gtaagaaaaa gaacaaaaca attacgagaa tcaaatagac aactttcatt atcaaatcaa
     1561 gaaattgtac aggctaatga agaattaaaa gttcatgata aaatgcaaaa ggaatttatc
     1621 aacgtcgcaa gccatgagat aaagacaccc cttcaagcta ttatttcagc ttcccaacta
     1681 ttacatatgt atccagaaag acaaaagaaa ttcgcgtcag cgatacaaag aaatgcgatt
     1741 aggctgcaaa gactttctaa tgatattttg gatgtagcta aaattgaaag caggtcatta
     1801 aaattaaaaa aagaattatt agatctaaat gaattaattt cgaatattat agaagattat
     1861 aagactatta ttataaaaga taatcaaaat gtcaaattat attttaaacc atcccacgat
     1921 cctttattcg tagaagctga taaggaaaga attgctggag taatttctaa ccttctcaat
     1981 aatgctatta aatttaccaa agaaggcgaa atatttgttt ctactgagaa agaagataat
     2041 agtaataacc cttatgc
//
LOCUS       DATAFW010000063         5763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_379288, whole genome shotgun sequence.
ACCESSION   DATAFW010000063 DATAFW010000000
VERSION     DATAFW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379288"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..679)
                     /locus_tag="VHJ57_01495"
     CDS             complement(365..679)
                     /locus_tag="VHJ57_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487240.1"
                     /translation="MVYHDKNREKLLIITSKIAKKTQNLRNKPVLYFSIDDENFPYKG
                     VKGKGLANIVEDPNRTVPEGDKISMKYLGTLDHPIAKMITEHSKKGENVLIEISPKFF
                     ST"
     gene            complement(685..810)
                     /locus_tag="VHJ57_01500"
     CDS             complement(685..810)
                     /locus_tag="VHJ57_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487241.1"
                     /translation="MKIINATKEAPGLTKEEIDKFLASKLMLQMSTIDEQGEPNI"
     gene            complement(987..1892)
                     /locus_tag="VHJ57_01505"
     CDS             complement(987..1892)
                     /locus_tag="VHJ57_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX2487242.1"
                     /translation="MKQLKLWIIIITLSLTVGSSFIAIKIVLDSIPPLFAFSIRFMIT
                     GVVLILVSYIFDRKSQEIKKITFWRNALLIGAFLILGGHGLIAYGAQYLSAGIASLLN
                     STIPLWVALFMFLIFHNKITNLTKIGVTLGFSGMIILVGPSIGGGSQGLSIVGIASLL
                     ISSLLWAIGSIYSSKVSLPQNFLLSAGMVTLIGGSLLLIPSILTGELNLLPSYSDIDF
                     SLLTSYLFLIFIGTVIPLAEFYWLLKVSTAAVANTFAYIAPIVAVFLGWAILNESVTY
                     LTIIATIVILIGVALIVRTSNNNNK"
     gene            complement(2307..2867)
                     /locus_tag="VHJ57_01510"
     CDS             complement(2307..2867)
                     /locus_tag="VHJ57_01510"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX2487243.1"
                     /translation="MELPWNDPRSNKFATTIGLITSDGPLRPNIMACEWTHHLSYDPG
                     LIAISLGFTKATVENIRSSKEFGVNLCAIDQSLLSSVAGGYSGSKYDKINAIRELGFE
                     FYEGSKIKSLMVKGASLNVECKLFQEVTCGDHIMFIGEVVDAINNPEKQSLIYHDGKY
                     WSLQSIVKPNQEERDRIRKVLESYKK"
     gene            3293..3697
                     /locus_tag="VHJ57_01515"
     CDS             3293..3697
                     /locus_tag="VHJ57_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HEX2487244.1"
                     /translation="MSRPNILENEQEIGSHAQIFIYRIPRKNHDAIVQLNKQIKDTLS
                     KHGPLHSEFFYLSSTETVMDFVNITETVSAKQDEEEVWVEIQSYRDRNHRDEVMVNME
                     KDKNCESLYQQYLDLITPGSRTIIGEFSRLKV"
     gene            3831..4796
                     /locus_tag="VHJ57_01520"
     CDS             3831..4796
                     /locus_tag="VHJ57_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX2487245.1"
                     /translation="MIYYLYLKSFLAVIICCSVFSLGFSSQSTIQNITDNSEKKIDYT
                     HYKITVNGIQLHYVIGGDGQGDPIVLLHGWPQTWYEWRYIIPQLIANNYTVIAPDMRG
                     LGDSEKPQTGYDKKTVAEDIYQLVKKLGYNKIYIVAHDMGGPVAYSYAAAHPQDVRKM
                     VILDTLLPGFGVEEAGNFSPNGIWHLSFHAVRDLPEKLIDGQENVYLNWFYDHYSYNQ
                     SAITPEDREEYIKQYSKPGAMRAGFEYYRTIFEDAEQNKEYAKQKLDIPILTIGGEAA
                     IGNFTTISFQKVANNVTGITLPNTGHFIPEERANFLTKQILDFFK"
     gene            complement(5432..5506)
                     /locus_tag="VHJ57_01525"
     tRNA            complement(5432..5506)
                     /locus_tag="VHJ57_01525"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5469..5471),aa:Val,seq:tac)
BASE COUNT         2048 a          884 c          870 g         1961 t
ORIGIN      
        1 ttcatagaac aggcatacaa aagaaatatc aacaagaact tcaactagat gggtccatta
       61 acaatcaata ataatttatt aaatagtatc ttaacatcat tgttagtata ataatattca
      121 tgatttctga ttagctagag cggcaactgt tatatcaaac tcaaaaaagt agtaaatagt
      181 aaaaacaaga tagaataaga cagtataatc gtatttattg ttgataatgg gtaagacatt
      241 taatctcatt aaatcatata atatttataa agatgattgt atttttgtgg tcatcaacct
      301 gagggaaata agaggtaatg aaaaacaact tagatatgag tcataactgc tctttaccaa
      361 aatcctatgt agaaaagaac tttggactga tttcaataag tacattttct cccttctttg
      421 aatgttctgt tatcattttt gctattgggt gatcaagtgt acctagatat ttcatactta
      481 ttttatctcc ttctggaacg gttctatttg gatcttcaac tatattagcc aagcctttgc
      541 ctttcactcc cttgtatgga aaattctcat cgtctatgga aaaatataaa actggtttat
      601 ttcggagatt ttgagttttc ttggctattt tagatgttat tatcaagagt ttttctctat
      661 tcttatcatg gtaaaccata caggttagat attgggttcg ccttgctcat cgatggttga
      721 catctgcaac atcaatttac ttgcaaggaa tttgtcgatc tcttctttgg tcaagccagg
      781 agcttcttta gttgcattaa tgattttcat aatacacttt atcggaatac aggcagaaaa
      841 gagtttcttc ctttgcttag ttgtatttga ataagtatac aaaaaagagg caccatacca
      901 tctatatttt cttttgataa cgataagttt atcctaaaat cacatattca tcgttttacc
      961 aaaattgttt tacgaaagat ttacaattac ttattattat tatttgatgt tctaactatc
     1021 aatgctacac ctatcaaaat aacaattgta gcgataatag ttagataggt cacagattca
     1081 ttcagaatag cccatcccaa aaagactgct acaataggag ctatataagc aaaagtatta
     1141 gctaccgctg ctgtactaac tttgagtaac caataaaatt cagctaatgg tataacagtt
     1201 cctataaata ttaaaaatag gtaggatgtg agtaagctaa agtctatatc tgaataagaa
     1261 ggaagcaaat ttaattctcc tgttaaaata ctaggaataa gtagtaacga cccgccaatt
     1321 aatgtaacca ttcctgcaga taataaaaaa ttttgaggca gggatacttt actagaatag
     1381 atagagccta ttgcccaaag tagtgaactt attaacaatg atgcaattcc tactatactt
     1441 aacccttgag atcctcctcc aatcgatggt ccaacaagaa ttatcatgcc gctaaaacca
     1501 agagtaacac ctatctttgt taaatttgtt attttattgt gaaatatcag gaacataaat
     1561 aatgctaccc ataaaggaat tgttgaattt agtaaagagg caattcctgc agagaggtat
     1621 tgtgctccat aggctatcaa tccatgtcca ccaagtatca gaaacgcccc aataagcaaa
     1681 gcatttctcc aaaatgtaat ttttttaatc tcttgacttt ttctatcaaa aatataagaa
     1741 acaagtatta aaacgacacc tgtaatcata aatctaatac taaaagcaaa taaaggtggt
     1801 atagaatcaa gaacaatttt gatagctatg aaacttgaac ccacagttaa agacaaagta
     1861 ataattatga tccataactt taactgtttc atagatttag gatgatctta taatattaaa
     1921 gttatataat gcattatcta cctaactagg aagtgaaata ctgcaacttt tgaaaaaatt
     1981 cacatattaa ccaatctagg taaaaatatt gtccataata tagttgaata atatatataa
     2041 tcttttatac tttgtaaaca taatatttag agatttataa ataatcacag ttaataccaa
     2101 atttaaagca tcatccccat catagaactt aaactctaga aggcgccttc tagaactaga
     2161 atatattcaa aaaatataaa ctttgtagta taattatagt cataaatctt gtagatagtt
     2221 ttgttctgtg gtcgaggtac tattagtaaa gtaaataatt tataacgttc atatggcgta
     2281 agttgagaat atcaaattct atattttcat tttttgtagc tttccaaaac cttacgtatt
     2341 ctatcacgtt cttcttggtt tggttttact attgattgta aagaccaata ctttccatca
     2401 tgatatatca atgattgttt ttctggatta tttatggcat ccactacttc tccaataaac
     2461 attatatgat ctccacatgt aacttcttga aatagtttgc attcaacatt aagcgaggct
     2521 ccttttacca ttaatgactt tatcttacta ccctcataaa attcaaatcc taattctctt
     2581 attgcattaa ttttgtcata tttactacca ctatatccac cagccacact agataatagt
     2641 gattgatcaa tagcacacaa gttaacacca aattctttag aacttctgat attctcaact
     2701 gtggcttttg taaaacctag agaaatagct attaatccag ggtcatacga aagatgatgt
     2761 gtccattcac aagccataat atttggacgt aatggtccat ctgacgttat cagtccaatt
     2821 gttgttgcaa acttattaga tctaggatca ttccaaggta attccataca gcataattga
     2881 ttaaaggatc ataaaaaatt tctgaattca taaaaattgt accatactat tttatttttt
     2941 agattatgaa atagagtctg gaaaataaat ctttgttctt ttcaactata taaaagtagc
     3001 ctattttaga tctgatatat attttatgta gtaacaaatt tatcattcaa actcttggtt
     3061 catactaaaa ccgatcttat agtagattac agaaattatt agaatcacag actacagact
     3121 agctaaagat aagaagggat ttgaactctt acttctgttc atggaattac ttttttgatt
     3181 atctttaatt atatttcagc tatttatctc attgaccttc tttgatgaat tgcaactaac
     3241 tatactttta ctcccttact tatttttata gtatattttg tatagtataa taatgagtag
     3301 accaaacata ctggaaaatg aacaagaaat aggaagtcat gcccagatct tcatttatag
     3361 aatacctaga aaaaatcatg acgctatagt acagctcaat aaacaaatta aagacacatt
     3421 atcaaaacat ggaccccttc actcggaatt cttttatctc agcagtacag agactgtaat
     3481 ggactttgta aacataacag aaactgtatc ggctaaacag gatgaggaag aggtttgggt
     3541 agaaatacaa tcttacagag accgcaatca tcgagatgaa gtaatggtaa atatggaaaa
     3601 agataagaat tgtgaatcac tttaccaaca gtatctagat cttatcactc ccggatctcg
     3661 tactatcata ggagaattca gtcgccttaa agtctagatt ttatagatta tagcaatata
     3721 tgatgaatga tataaagtgg aaaatcgata tgaactggaa tctaaagtcg aaaacagaaa
     3781 tagttgtatg taattatgtg actaacatat cataggagat cgatattcta ttgatatatt
     3841 acctatacct taaaagtttt ctggcagtca tcatttgttg ttcggtattt tctttaggat
     3901 ttagttctca atctacaata caaaacatta ctgataatag tgagaaaaaa atagactata
     3961 cacattataa aattacagtt aatggaattc aacttcatta tgtgataggt ggtgatggtc
     4021 aaggagaccc gatagttttg ctgcatggtt ggccacaaac atggtatgaa tggcgataca
     4081 ttattcccca attaatagcc aataactata cggtaatagc tcctgacatg agaggtttag
     4141 gagattcaga aaaaccacaa acaggatacg acaagaaaac tgttgcagaa gatatttatc
     4201 aattagtaaa gaagttagga tataataaaa tatatatcgt agctcatgat atgggaggac
     4261 cagttgcata ttcttatgct gcagcacatc ctcaagatgt acgtaaaatg gttatactag
     4321 atactctatt acctggattt ggagttgaag aagcaggaaa cttttcacca aatggaatat
     4381 ggcatctttc atttcatgca gtaagagatc tgccagagaa gttgattgat ggacaagaaa
     4441 atgtatatct taattggttt tatgatcatt attcttacaa tcaatctgca ataacaccag
     4501 aagatagaga agagtacata aaacaatatt ctaaacccgg tgcaatgcgt gcaggttttg
     4561 aatactatag gacaattttt gaagatgcag aacaaaacaa agagtatgct aaacaaaaac
     4621 tagatatacc aatattgaca ataggtggag aagcagctat aggcaacttt actaccatct
     4681 catttcaaaa agttgcaaat aatgttacag gaataacgtt accaaataca ggacatttca
     4741 tcccagaaga aagagcaaat tttctgacaa aacagatact tgattttttc aaataaaacc
     4801 ttttttatat ttgtagtttt ggtattattg gaacttacct agttcactta ttgatataat
     4861 gtattagttg gatcaaaatt taatcaggat tatcgaatct ggttcagagg tatgtgaatt
     4921 gattttgaca tgagttttgt tattcttaag ggataatcat caatagcatc tttattcgca
     4981 gctagatatt tgattcataa ctgaatcaaa gagacgaact ttttttgcat ttaggtttac
     5041 actagtcttt gataccaatc actgtctaca aatattattc aatatttttc tatagctaat
     5101 ttttgttatt ttttcaaaag ctttgtaatg aatgtaaaca tatggctttg atatgagtta
     5161 aaaaattttt ttgaagttta acattttgat aattctgttc tgttattaga aattcataag
     5221 agtttttgca ttttgtaaat ttttaaaaaa ctttttttta aaaagtgaat tgtaatgaga
     5281 tgaagctcct ttacatagga attaatcttt ttttctaaat ctagaatgat agtatgggca
     5341 tcggtattta cacaactttg agccattact ttaacgtcga tagtttgctt ttccttcttt
     5401 ataaaaagta tatataatca catgaacagc atggagggga tgggatttga acccatgacc
     5461 acctgcgtgt aaggcaggta tcctaaccag tctagacaac ccctccgtta ttgatctttg
     5521 attcattgtt ataactatta tattaattta tgattgattt ttcatagatt ttggaatcat
     5581 attcaatatt gtttttaatt acaacatttt taaattagga aggaaatcca aaaactatac
     5641 taatgagtgc aaaagaatcc tataataaaa tctttgagtt aatgaaaagc catcatgatt
     5701 ggtttgctaa ttctattcca ttaattgcta gtgagaatat tcctagtaca gcagtaagag
     5761 agg
//
LOCUS       DATAFW010000064         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6017634, whole genome shotgun sequence.
ACCESSION   DATAFW010000064 DATAFW010000000
VERSION     DATAFW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6017634"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VHJ57_01530"
     CDS             complement(<1..143)
                     /locus_tag="VHJ57_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX2487246.1"
                     /translation="MGKYELPPVPYSYDALEPYIDARTMEIHHTKHHQAYTDKLNAAL
                     EKCS"
     gene            267..722
                     /locus_tag="VHJ57_01535"
     CDS             267..722
                     /locus_tag="VHJ57_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HEX2487247.1"
                     /translation="MKIKILFKRLLMVINKELLEKRKKISEHRPNFVRQESWRYDRLA
                     ENWRKPKGKDNKMRRQVSGVPQLVKVGYRGPKAARGLHPSGYVDNLVYNTNDLLSLDS
                     KKDAVRIGHIVGKLKKKQILTKAAELGFKVLNPGKDSKSQNKSQIKENG"
     gene            725..1171
                     /locus_tag="VHJ57_01540"
     CDS             725..1171
                     /locus_tag="VHJ57_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HEX2487248.1"
                     /translation="MVVNLKKKRELVARMLGVGSNRVKFEPDKLDDIADSITREDIRS
                     LVKNGVIWTSKPKGTSRGKAKAKLDIKKKHGTGAGSKKGRKTARTGKKELYVKKIRAM
                     RYHLKVLKDRNDVNREIYWSLYKRINGGQIRSLSHLRDTVKDWKSQ"
     gene            complement(1201..1398)
                     /locus_tag="VHJ57_01545"
     CDS             complement(1201..1398)
                     /locus_tag="VHJ57_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487249.1"
                     /translation="MSNWEENNQDDRDDLNILTLPNIKYIVEGLNVLLQTNLDGEKRS
                     QVINLREKLINRSSSIINDFN"
     gene            1502..>2104
                     /locus_tag="VHJ57_01550"
     CDS             1502..>2104
                     /locus_tag="VHJ57_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX2487250.1"
                     /translation="MFTLITSEIDPASRTMIKYLQEDKKFKEIKNYYFTSPLYDNISM
                     FVSPRQLLFFDSMDVECNDSADVLIFLSRHQSKSKIPSLTCHFPGNYFTNSYGGNNFE
                     LGIAYPSLQKNYLLKIFQLREQVPLCDITIETTHHGPTSIKKPVMFIEIGSSEDQWIN
                     LENASFVCNSLLAILTKKISPAKDIAIGLGGNHYGSKFNRL"
BASE COUNT          768 a          283 c          357 g          696 t
ORIGIN      
        1 gaacatttct caagtgctgc attgagttta tctgtataag cttgatggtg ttttgtatga
       61 tgtatttcca tagttcttgc gtcaatatat ggttctagtg catcataact ataaggtaca
      121 ggcggtaatt catattttcc catacatgta tttttttttg taattattaa cctttgtgtt
      181 atctaattta attttctaac attgagttga ttttatacct actttctttt tatacctact
      241 ttctttctct gagggaaaca tatttattga aaattaaaat tctatttaaa cgcttactca
      301 tggttattaa taaggaactg ctagaaaaac ggaaaaaaat atcagaacat cgaccgaatt
      361 ttgtaagaca agaaagctgg agatatgaca ggcttgcaga aaactggagg aagcctaaag
      421 ggaaagataa taaaatgaga cgacaagttt ctggcgtccc tcaattagta aaagttggat
      481 atagaggtcc aaaggccgct agaggacttc atccatcagg atatgttgat aatttagtct
      541 acaatactaa tgatctgtta tcattagatt caaaaaaaga tgctgtaaga ataggacata
      601 tagttggaaa attaaagaaa aaacaaattt tgactaaagc agctgaattg ggttttaaag
      661 ttttgaaccc aggaaaagat tctaaatcac aaaataaatc acaaataaag gagaatggat
      721 agatatggtt gtaaatctta aaaagaaacg tgaattagtc gctagaatgc ttggtgttgg
      781 ttctaatcga gttaaattcg aacctgataa gcttgatgat atagcggatt ctataacgag
      841 agaagatatt agatctttag ttaaaaatgg agttatatgg acatcaaagc ctaaaggaac
      901 ctctcgaggt aaagctaagg caaaattaga tataaaaaag aaacatggaa ccggagctgg
      961 atctaaaaaa ggtcgtaaaa cagcaagaac aggaaaaaaa gagttgtacg taaaaaaaat
     1021 aagagcaatg agataccatt tgaaagtatt aaaagataga aatgatgtta accgtgaaat
     1081 atattggtct ttgtataaaa gaattaatgg tggtcaaatt cgatccttga gtcacttaag
     1141 agataccgtg aaagattgga aatcacaata aagggttcaa aagaaaaaat aatatttgaa
     1201 tcaattaaaa tcgtttataa ttgaactgga cctattgatt agtttttctc ttaaattaat
     1261 aacttgggat ctcttttctc catccaagtt agtttgaagt aatacattta atccttcgac
     1321 tatgtatttt atattgggta aagtaagaat attgagatca tcccgatcgt cttgattatt
     1381 ttcttcccaa ttactcatac tttaagatgt tttgcgttaa tgttaaatgt tttggatttc
     1441 aagttaataa attaattgat gtagttacta aaatattata gttttagatg atgatcatat
     1501 gttgtttaca ctaattactt cggaaattga tccagccagt agaacaatga taaaatattt
     1561 acaagaagac aaaaaattta aagaaataaa aaattactat tttacttctc ctttgtacga
     1621 taatatatcc atgtttgtat ctccacgcca gttactattt tttgatagta tggatgtcga
     1681 atgcaacgac tctgctgatg ttttgatttt tctttctaga catcaatcaa aaagtaagat
     1741 tccatctttg acctgtcatt ttccaggaaa ttattttaca aattcttatg gcggaaacaa
     1801 ttttgagtta ggaattgcat atccttcatt gcaaaagaat tatcttttaa aaatattcca
     1861 attaagagaa caggttcctt tgtgtgatat aactatagaa actacacatc atggtcccac
     1921 tagtataaaa aaacccgtca tgtttattga aataggttcc tcagaagacc aatggattaa
     1981 tcttgagaat gcatcatttg tatgtaattc tttattagct atattaacaa aaaagatttc
     2041 tcctgcaaaa gatattgcaa taggattagg aggaaatcat tatggttcca aattcaatcg
     2101 ccta
//
LOCUS       DATAFW010000065         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_381763, whole genome shotgun sequence.
ACCESSION   DATAFW010000065 DATAFW010000000
VERSION     DATAFW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381763"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1584
                     /locus_tag="VHJ57_01555"
     CDS             208..1584
                     /locus_tag="VHJ57_01555"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX2487251.1"
                     /translation="MSINTKVNLYYDKGSIVISGINQIPYSLIDPRTNQLRAQALHYQ
                     DIITYLQNSGIGYNNNVMDLIPLPNLKIENIQLSLRSYQKKAIENWITAGKKGCIILP
                     TGSGKTIIAIKLIEIVNSSTLIVVPTLDLMEQWTEFLSKYFQKIEIGNIGGGTSNITG
                     ITVSTYDSAYIRSSFIGNKFAFVIFDELHHLAAPGYRTIAEQFIAPYRLGLTATYERE
                     DNLHLDFPRLVGGIVYQSFVNELAKDKHIASFIVEKRYVKLLPEEEIEYHKNYNQYLS
                     YLRELGIRYGQIGFQKLIMISGKNQYARNALLARNKALDIALNSESKIEELRKILIEN
                     RHTKTIIFTQHNKLVYLISNKFLIPFITHKSSKQEREDALNGFRDGRYRVLVTSKVLD
                     EGVDVPDAELGIIVSGTGSKREFIQRLGRLLRTKPNNKNAKLIEIISSGTSEINTSIK
                     RKQALKNI"
     gene            1833..4100
                     /locus_tag="VHJ57_01560"
     CDS             1833..4100
                     /locus_tag="VHJ57_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2487252.1"
                     /translation="MSDFRNKKEERIHEMQRFIVHPLIKNNSIESRLYQNIIFNSCKD
                     KNSLIVLPTSLGKTVISALICAEILFNYKQNKILIMAPTRPLVLQHLLSFSSFLKVLD
                     DQKILVTGKISPSTRKMVWDNKTIKLIFATPEVIRNDLNESRLNLKDFGLIVFDEAHR
                     AIKDYAYTLISKKYMEQSPNPLIVAMTASPGSEKNRIEQLCKNLYIEHIEYRNEEDVD
                     VKPYVNPIELKWEFTELSEEYRQIIVLFKSMLQEKLRWLIFRGLIKKNNIDYVFKRDL
                     LELGEILQRNLKFTLNEEKGPLYLALLNQSAALSLMYCIELIESQGPFSLKTFIKRME
                     SSEGKAHSLILKDPRMKDIFKLLENVTEHPKLIYILDLCKKEKLDTYANKNSNVLLND
                     KSPQILIFSHYRDTATHIVDELNKTGIKSFRFVGQSTRNNDIGMNQTEQSAILDSFRK
                     KEFNVLVATSIAEEGLDIPEVKVVIFYEPVASEIRHIQRKGRTGRKSSGNVIILAAKD
                     TVDMRILFANKRRIEKMKTIFTSIKTILNPISRLPIPYNRMTELELADLDKNLNIEQK
                     KLNGKELKYEDSERKVIEKIKVKNAIIDQIPKLQIGTITKTENNLIEKAYRHIYNFLI
                     DERNNKNSSLSSSSFDLSYLNEKFTFDTHIILQALKKLEKQQIIKIKNNKLITLNRLP
                     PNKSTGREYNIYVEKILPGKAYVLVNEKWYALLNYYDYEGPRNLLKKGSEFKAIAELY
                     TNENNFCIRIKEIIF"
BASE COUNT         1713 a          519 c          600 g         1342 t
ORIGIN      
        1 ccatttcctt atttttatgc tgttggaatt ccagtagata atgtaataat gcaacctgtt
       61 cttattgcca ctgccatact cttaattcca tttattttta tgatgtttgc atggagaaaa
      121 agccctacat attaaattat gatatataaa ttcaagatat atattgaatg cgatttcttt
      181 ttaagagata attgtttaat atattaattg tctataaata ctaaagtaaa cctttactat
      241 gataagggtt ctattgttat ttctggaatt aatcaaattc cctattcatt aatagatcca
      301 agaacaaatc aattacgagc acaagcatta cattatcaag atattattac atatttgcaa
      361 aatagtggta ttggatataa caataatgtt atggatttaa ttcctcttcc aaatttgaaa
      421 attgaaaata tacaattatc attaagatcc tatcaaaaaa aagcaattga aaattggatt
      481 actgcaggaa agaaagggtg tataatatta cctacaggtt caggaaagac tataatagca
      541 atcaaattaa tcgaaatagt aaattcttct acactgattg ttgttccaac cttggatctt
      601 atggagcaat ggacagaatt tttatcaaaa tattttcaaa aaatagaaat tggaaatatt
      661 ggtgggggta catctaacat cacgggtatc actgtatcta catatgattc agcttatatt
      721 cgctcatcat ttataggaaa caaatttgca tttgtgatat ttgatgaatt acatcatttg
      781 gctgcaccag gatacagaac tattgcagaa caatttatag ctccatatag attaggtttg
      841 actgctacat atgaacgtga agataatcta catttagatt ttcctagatt agttggagga
      901 atagtatatc agtcatttgt taatgagtta gccaaggata aacatattgc atcttttata
      961 gtagagaaaa gatatgttaa attattacct gaagaggaaa tagaatatca caaaaattac
     1021 aatcaatatt tgtcttattt acgggaacta ggaattagat atggtcaaat tggatttcaa
     1081 aaattaatta tgatatcagg aaaaaatcag tatgctcgaa atgcattact ggcaagaaat
     1141 aaagctttag atattgcatt aaatagcgaa tctaaaattg aagaattgcg taaaattctt
     1201 attgaaaatc gacatacgaa aacaattata tttacacaac ataataaatt agtttattta
     1261 atttctaata aatttctaat tccttttata actcacaagt ctagtaaaca agaaagagaa
     1321 gatgcattaa atggttttag agatggcaga tatagggttt tagtaacttc aaaagtttta
     1381 gatgaaggag ttgatgttcc tgatgctgaa ttaggaataa tagttagtgg tactggaagc
     1441 aaaagagaat ttattcaaag acttggacgt ttactaagaa caaaaccgaa taataaaaat
     1501 gcaaaactta tagaaattat atcttcagga acaagcgaaa ttaatacaag tattaaacga
     1561 aaacaggctt tgaaaaatat ataattatat actttaaaaa ttattaatac aataataatt
     1621 cttatgttcc tattatatat tactaataat aaatattatt ttatgtttta tatttcggat
     1681 aaatagagat cattttagta atatttttta taatcataaa aaatattaaa tatatttcat
     1741 atttatcaat aataaagtat tataaaaaat aaaaacaaaa agaattaaat tatttagtac
     1801 aatatattgt ttgtaataaa tacatttgtc atatgtcaga ctttagaaat aaaaaagaag
     1861 aacgcataca tgaaatgcag agattcatag tacacccact tataaaaaat aattctattg
     1921 aatctagatt gtatcagaat attattttta attcatgcaa agataaaaat tcgttaattg
     1981 ttctccctac ctctctaggt aaaactgtta tttctgcact gatctgtgct gaaattttgt
     2041 ttaattataa acaaaataaa atcttaataa tggcaccaac tcgccccttg gtattacaac
     2101 acttgttatc tttttcttca tttcttaaag tattggatga tcaaaaaata ttggtaacag
     2161 gtaaaatttc tccttcaaca cgaaaaatgg tatgggataa taaaacaata aaattgattt
     2221 ttgctacacc cgaagtaata agaaatgatt tgaatgaatc acgtctaaac ttgaaggatt
     2281 ttggattgat agttttcgat gaagctcata gagctataaa agattatgct tacactctta
     2341 tttcgaaaaa atacatggaa caatcaccaa atcctcttat tgtagctatg actgcaagtc
     2401 ctggctctga gaaaaacaga atagaacaat tatgtaagaa tctttacata gaacacattg
     2461 aatatcgaaa tgaagaagat gttgatgtca aaccatatgt gaaccctata gaattaaaat
     2521 gggaatttac agaattatca gaagaatata gacaaattat agtattattc aaatctatgt
     2581 tacaagaaaa attacgatgg ttaattttta gaggattaat aaaaaaaaat aatatagatt
     2641 atgtatttaa aagagattta ttagaacttg gagagatttt acaaagaaat ttaaagttta
     2701 ctttaaatga agaaaaagga ccgttatatc tagctctgtt gaatcaatct gctgctctat
     2761 ctttgatgta ctgtatagaa ctaatagaaa gtcaaggacc attttctcta aaaaccttta
     2821 taaaaagaat ggaatcaagt gaaggaaaag cacattcatt aattctgaaa gatcctcgaa
     2881 tgaaggatat tttcaaacta ttagaaaatg ttactgaaca tcctaaactc atttatattc
     2941 ttgatctatg taaaaaagaa aaattagata cttatgcaaa taaaaactcc aatgtattat
     3001 taaatgataa gagtcctcaa attttgatat tttcacatta tagagacact gcaactcata
     3061 ttgtagatga attgaacaaa acgggaatca aatcatttag atttgttggt caatctacta
     3121 gaaacaatga cattggaatg aatcaaactg agcaatctgc tattttggat tcatttagga
     3181 aaaaagaatt taatgtactt gttgcaacat caatagcaga agaaggatta gacattcctg
     3241 aagttaaagt agttatattt tatgaaccag tagctagtga aattagacac atacaaagaa
     3301 aaggtaggac tggaagaaaa tcaagtggaa atgtaataat cctagcagca aaagatactg
     3361 ttgatatgcg tattttattt gcaaataaga gacgaataga aaaaatgaaa acaattttta
     3421 cctcaataaa aacaattcta aatccaattt cacgactccc gataccatat aatcgtatga
     3481 cggaattgga actagcagat cttgataaga acttaaacat agaacaaaaa aaattaaatg
     3541 gaaaagaatt aaaatatgaa gatagtgaaa ggaaagtaat tgaaaaaatt aaggttaaaa
     3601 atgcaataat agatcaaatt ccaaagcttc aaattggaac gataacaaaa actgagaata
     3661 atttaataga aaaggcatac agacacattt ataatttttt aatagatgaa agaaataata
     3721 aaaattcatc attatcatca tcatcatttg acttatccta tcttaatgag aaatttacat
     3781 ttgatacaca tattatatta caagccctaa agaaattaga gaagcaacag ataattaaaa
     3841 taaaaaataa taaattaata actttaaatc gattaccacc aaataaatca acaggaagag
     3901 aatataatat ctacgtggaa aaaattttac ctggcaaagc ctatgttcta gtaaatgaaa
     3961 aatggtatgc cttgctaaac tattatgatt atgagggacc tagaaacctt ttaaaaaagg
     4021 gatcagaatt taaagctatt gcagaattat atactaatga aaataacttt tgcattagaa
     4081 taaaggaaat aatattttaa taatgttcta gaagaagaaa tatgggattg gagataccgc
     4141 agacacttca gtcaacaaat atctccttat tcgg
//
LOCUS       DATAFW010000066        10793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4081476, whole genome shotgun sequence.
ACCESSION   DATAFW010000066 DATAFW010000000
VERSION     DATAFW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4081476"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   472..776
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=472..502
                     /rpt_unit_seq="agtttcaaacctattttagtcaaattcaaac"
     gene            1077..1280
                     /locus_tag="VHJ57_01565"
     CDS             1077..1280
                     /locus_tag="VHJ57_01565"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEX2487253.1"
                     /translation="MIFSCRETGLDCDYTIKGESEEELLKIGAEHVINVHGMKVEDIF
                     FKNIPCNFLCHTLSKINEKDVKD"
     gene            complement(1744..3438)
                     /locus_tag="VHJ57_01570"
     CDS             complement(1744..3438)
                     /locus_tag="VHJ57_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX2487254.1"
                     /translation="MSKRHSSHLFSFSAGLVVGLVSISISIIFKVFAGGLFLPELASQ
                     TLFSLTPGEFESQAIETLGPLAKYSTFIASIIVTTIIFGIFGIILDKLFYKFNLLNQY
                     ILKAISSFTLTYGTLVIISLILVTIIQLRSGSQTISIPFVILSLIPSQIAAGLIFPLF
                     LRKSKDKGERQPYPPQQQKITENTKEEHYNRRDFIRLVLASVVSIPIIYFGLNRLFSP
                     LQDIEQQQQQQQIQSNISNLLRSQSQQQRSIPAEFKDPALAPLLGSEITPTYLFYRID
                     INPIVPEVAVNSWNLQVKGMVDNPLKLSYEEIRSMPAIEEFVTLECISNKIGGDLTGT
                     ALWKGVPLKDILQKAKIASSVKYIVFRCSDGYDVGIPLDKGLMEETILAYEMNLAPLT
                     SKHGFPLRAIVPGLYGMMNPKWITEIELVDKVYEGYWQRNGWTNIAEYNTGSSIVIPG
                     QAPIRHRFRGLDEIPSSYSSNGRVPIAGIAFGGNRGISKVEVSTDGGKTWKTAKIKEP
                     LSQYTWVLWTAGFIPKERVGNYKIVVRATDKKGQVQISELRKPFPDGATGYHTISV"
     gene            complement(3609..3986)
                     /locus_tag="VHJ57_01575"
     CDS             complement(3609..3986)
                     /locus_tag="VHJ57_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487255.1"
                     /translation="MMKEKEFTFIQMISLIIFGLALNLINSVSFKALALTEDPITTTQ
                     YDNELVNGNNSMMLPGSNMTFGSSLDNAKMHLMEAIMDLEDNNIDGALMQLNMTSKDI
                     KMHEQEMADMMLMINKMKEVDKK"
     gene            4276..5574
                     /locus_tag="VHJ57_01580"
     CDS             4276..5574
                     /locus_tag="VHJ57_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX2487256.1"
                     /translation="MHRRGSNSYLSIQTAFIKLRKSIIIQPMSEELDTKEALGRVLYN
                     NIISSINIPMYDSSHMDGFAVLHEDLKGASNLKPITLKVIDNIKLGNTKIKPLQSGHA
                     SRIPTGGYLPKNSDTVVPIEYVQFNLFNKSVKIFSEFPKRSFISPAGMDISKNKLLLK
                     KGHLIRTQDIALLNLLSIKKLNVFKKPRVAIIPTGNELTNNIDEVIQYGKVLNTNGQI
                     ISSLIEASGGIPIDLGIASDNIKDIKKKMEYAISQNDIIVTIGGSSVGHKDLIAESID
                     SMGEPGIFAEGIKLDRGRVTKIAILKSKPIIILPGPIQGAVNAFIVLVMPLIRSLIGL
                     SHNNQAIINAQISDNWNARKKFQHFVKILYVHLSISKANHKINAEPIIGETANMTVMT
                     KANGYVMIPEKITSIEKGNEIQVNLLSGFSFASRNPIDLI"
     gene            complement(5579..6397)
                     /locus_tag="VHJ57_01585"
     CDS             complement(5579..6397)
                     /locus_tag="VHJ57_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX2487257.1"
                     /translation="MHLICWEGKVINEILLLVLAPIFFVISFFYSSVGFAGGSSYIAI
                     LVLVGVNLYSVPPISLALNIIVSSAAFFNYLRAGHLSLRLSIPLFISVPFAFLGGSIV
                     LPEKILTLIFVISLFFASASLLLSGRRRRRRITHQYEKMKKMNLNIIKTTLITCPLGA
                     LLGSISGLVGIGGGIWLSPILIMSGLANPKEAAASASLFILTNSISGFLAHSITNSKD
                     VNFDLIIPLSIVVLTGGIIGSMLGAFKFDHTKIIIIIGILVAVAAINLSTRLFK"
     gene            complement(6363..7319)
                     /gene="moaCB"
                     /locus_tag="VHJ57_01590"
     CDS             complement(6363..7319)
                     /gene="moaCB"
                     /locus_tag="VHJ57_01590"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEX2487258.1"
                     /translation="MNKTTINNYNKGMFDVSNKADTLRFAIAQAIIKLSSETVDLIKK
                     QKMPKGDIIEAAKISAALGAKKTWELIPYCHPIPIDHISTQLSLYESIIEIKVSVKSV
                     WKTGVEMEALTGCSIAALAVYDMLKPIDEHLSIESIKLLDKIGGKRDFIEKNLDKKIT
                     VAIFVISDSTFNGTRVDKSGKMISDRLKNTYATNTEIIGYEVLPDNIDKIKTYIIKYS
                     DEKKVDLIITSGGTGFSKRDVTPEATKMTIDKETNGISEAIRSYGQRRTPLSMLSRGI
                     SGTRGKTLIINLPGSTKAVSESLDLLLPSIFHAFNMLGGKGH"
     gene            complement(7312..7734)
                     /locus_tag="VHJ57_01595"
     CDS             complement(7312..7734)
                     /locus_tag="VHJ57_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX2487259.1"
                     /translation="MISIIKDTIDQLKILEELKDNSSGAVVFFTGIVRDHNSKEKVEG
                     IFYEAYEEMVNEILKKIENEVFKNWNIKKFIAIHRIGYLKVGEISVIVGVSSEHRKEA
                     FEACSYGIDNIKKRCPIWKKEKMESGDSEWVEGVMINE"
     gene            complement(7755..8024)
                     /locus_tag="VHJ57_01600"
     CDS             complement(7755..8024)
                     /locus_tag="VHJ57_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX2487260.1"
                     /translation="MNNSCEDRTLKLKVFGILREKLKSNTLSIPINNNSISLEELKNC
                     LKELDPHLSMNEINFVFAVNKVICNENVKVSSSDEIALIPPISGG"
     gene            complement(8034..9074)
                     /gene="moaA"
                     /locus_tag="VHJ57_01605"
     CDS             complement(8034..9074)
                     /gene="moaA"
                     /locus_tag="VHJ57_01605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX2487261.1"
                     /translation="MVKKFNLISTNPPKNLKDSYNRIARKLRISVTDKCNMKCIYCMP
                     SGNIKWIENKEILNFKEIIRIVSILTQLGITKIRLTGGEPLLRPSLENLISDLIKIEN
                     IETISMTTNGFLLEENINKLKSAGLNSLNISLDTFREERFRVLNGISNLNKVLRGIQK
                     ARELGVKIKINTVIIRNWNDDEIINFVKFARDMDITVRFIEFMPLDGSGIWNYNLVMT
                     KKEIIKIIETNFHNVISLDKNNSYTSDPATIYNFDDKKGKIGFIPSITDPFCSNCDRI
                     RLTSDGRFLTCLFEKEGYDLKKMIRNAKITDQEIREYIVNCYKMKPEGIVKLIRINQL
                     RPTLNLMHTIGG"
     gene            complement(9087..9737)
                     /locus_tag="VHJ57_01610"
     CDS             complement(9087..9737)
                     /locus_tag="VHJ57_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX2487262.1"
                     /translation="MKVVSVNVGLPRIVKFGKETVTTGIFKNPVDKRINLKKLNLEGD
                     RQADLTVHGGPDKAVYSYPFEYYDFWKKEFPGISFNWGMFGENLTTEGLFESKVNVGD
                     IFQIGSSQIVVTQPRMPCYKLGIKFGRMDVIKKFLESEKSGIYFKVIKEGEIGTNDTI
                     KLIKKDDNNVTIKNIVELVTKEDKENTILMEKAIKVQDLPQGWKQYFLEKLSHPKK"
     gene            9847..9996
                     /locus_tag="VHJ57_01615"
     CDS             9847..9996
                     /locus_tag="VHJ57_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487263.1"
                     /translation="MTIRHSSSHRYGLWILAIGIVTMVFSISLFSSSSIAPSVVVNSR
                     KVLIG"
     gene            10005..10148
                     /locus_tag="VHJ57_01620"
     CDS             10005..10148
                     /locus_tag="VHJ57_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX2487264.1"
                     /translation="MCLICGHIGCCDSSPGIHATKHHETTGHPIMIALPDRSWKWYYI
                     HKQ"
     gene            complement(10468..>10793)
                     /locus_tag="VHJ57_01625"
     CDS             complement(10468..>10793)
                     /locus_tag="VHJ57_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487265.1"
                     /translation="FMKSEFIVSKIEASQDEKTPFVYVVFNDTKTPRRGNQQQPSFGG
                     TGMAFTSPEDLMKNLPKMFSGSGVDVGNSPTFKLSIREYDETGLRVGDKVTIEIKKSD
                     SRGGI"
BASE COUNT         3682 a         1645 c         1455 g         4011 t
ORIGIN      
        1 tagtaaaaat tttccaaatt gcaaaggttt atatactcta gtgtaaaatc ttgttttttg
       61 tgtatttata tctacatatt tcaatgaaat agaatgttat ttagttaaat ttgacttact
      121 atcgtgttgt aacctatcaa aataataaaa aaatgataaa atttatgtgt cctaggtact
      181 cgaaaacccc ccgtataatt tttcttaatt gcgtctatta acaaaaagtt tgcctagttg
      241 agtagatttt gaatttgtgc gtatattggc tggctcaata tggattaatt aaaggaaaat
      301 tattgtactt cggaacgata tcaaaacaat aacgccggaa gtggggactc gaaccctcca
      361 actacatggg tttatgttat tagaatatac aaaaagatta aatgaaattt tttggattat
      421 gctaaagcat tttcttaaaa ttattgttac agaattctta ggcatgttct aagtttcaaa
      481 cctattttag tcaaattcaa acgactgtta gaggacgatg aagaggttat ttcaaatatg
      541 atagtttcaa acctatttta gtcaaattca aaccaaacct ccaataccta caagtaatgc
      601 aacaatgcct agtttcaaac ctattttagt caaattcaaa ccggacaatt actagttgat
      661 ggtgctaatg tcaatttcag tttcaaacct attttagtca aattcaaact tttatcattg
      721 aagtatattc ctcttttgtc gacttggttt caaacctatt ttagtcaaat tcaaacttga
      781 cagtattata cgatgaattc atcaagttct cgtttcagat taactcttag ttttaagtga
      841 aagaattcag gaaatagaaa aaaaataatc aaataagatt taaattgatc tgcaaaaata
      901 tgatgtgtgg aatttgaacc cttgataaag tgggaccagt taaattaagt cttttcatag
      961 ttttagaaat attctgtatg ttatcgttta aatttcaata aatgcttaaa atctctgtta
     1021 ttactataat cagattaact tattaccttc taaattataa atatatctag gcagatatga
     1081 tattttcatg tcgtgaaaca ggattagatt gtgattatac aattaaaggc gaaagtgaag
     1141 aagaactttt aaagataggg gcagaacatg taataaatgt tcatggtatg aaagttgaag
     1201 atatattttt caaaaatata ccatgtaatt ttttgtgtca tacattaagt aagataaatg
     1261 aaaaagatgt gaaagattaa tttgcagatc ttttagataa tcaaaattta gataatacga
     1321 tttatatatt aagataatac gatttatata ttagaatatg ttattagata tatatttatt
     1381 gattcttctt attcaagcta gattacttga caataattaa gatttaaaat tatgtatgcc
     1441 ctaataataa aaaatgtttt tacataaatc acgatagtta tagtgtaaaa agttctaatt
     1501 gtaattggga agaattcacg tttggatatt gctaaagcta tctcattcat agatcatagt
     1561 gattatgata caaaggtcta ttgattattt ggatattaca ataattgaga taaactaaat
     1621 agaatatata atcaaatttt cgttgctgtc tactaaaatt taatatttaa ttagatattt
     1681 gtaaataaat tttagtagta ttaatatgta tttttatcaa atgattttag tataaatata
     1741 tacctataca ctaattgtat gatatccagt tgctccatct ggaaatggtt ttcttagctc
     1801 tgaaatttga acttgtcctt ttttgtctgt agctctcact actattttat aattacctac
     1861 tctttctttt ggaatgaatc ctgcagtcca caatacccat gtatattgag agagaggttc
     1921 tttgatttta gccgttttcc atgttttacc gccatcagtg ctaacttcga cttttgagat
     1981 acctctgttg cctccaaatg ctattcctgc aattggtact ctgccatttg atgagtaaga
     2041 agatggaatt tcgtctaaac ctctgaatct atgccttatt ggagcttgac caggtataac
     2101 tatggatgaa ccagtattat actcagcaat atttgtccat ccatttcttt gccaatatcc
     2161 ttcatatact ttatcaacta attctatttc ggtgatccac tttggattca tcattccata
     2221 taagcctggc actatagctc taagaggaaa tccatgttta gatgttaatg gagctaaatt
     2281 catttcatat gccaatattg tctcttccat tagtccttta tctaatggaa tacctacatc
     2341 ataaccatca gaacatcgaa aaactatata ctttacacta gatgcaattt ttgctttttg
     2401 aagaatgtct ttgagtggta ctcctttcca taatgctgta cctgtaaggt ctccacctat
     2461 tttattactt atacattcca aggttacaaa ttcttcgatt gcaggcatag atcttatttc
     2521 ttcatagctt agttttaatg gattatctac cattccttta acctgcaaat tccaagaatt
     2581 tacagctact tcaggaacaa taggatttat atctattcta taaaatagat aggtaggtgt
     2641 tatttctgaa ccaagtaatg gtgcaagtgc tgggtcttta aattcagccg gtattgatct
     2701 ttgttgttgt gattgtgatc gcaataaatt tgaaatatta gattgaattt gttgttgttg
     2761 ttgttgttgt tcaatatctt gtagtggtga aaatagtcga tttaatccaa aataaattat
     2821 tggaatagaa acaacagagg caagaacaag acgaataaag tctcttctat tataatgttc
     2881 ctctttagtg ttttctgtta ttttttgttg ttgtggtgga taaggctgcc gttctccttt
     2941 atcttttgat tttcttaaaa acaatggaaa aatcaatcca gctgctattt gtgaaggaat
     3001 aagagaaaga atgacgaatg gaattgaaat tgtttgagat ccacttctaa gttgaattat
     3061 tgttactaat ataagggaaa taataactaa ggttccataa gttaatgtaa aagaagatat
     3121 agcttttaat atgtattgat ttaacaaatt aaatttataa aataatttat ctaaaataat
     3181 cccaaatata ccaaagatta ttgtggtaac tattatcgat gctataaaag tagaatattt
     3241 agcaagaggt cccaaagttt ctattgcttg agattcaaac tctccaggag ttagtgaaaa
     3301 taatgtttgt gatgcaagtt caggcagaaa taatccacca gcaaaaactt tgaagataat
     3361 tgatatagat atagatacaa gtcctacaac aagtcctgct gagaaagaaa ataaatgtga
     3421 actatgtctc tttgacacat aatttattat agaataaatg atttaagttt tctttttttt
     3481 gggtatttta caaaaataaa ttgatggatt agaaataagc gctattaacc agaataaaag
     3541 agttctgtgt tctattggaa ctcgacttat aattttttta tatttgaaga aacgttctca
     3601 ttattatttt attttttatc aacttctttc atcttattta tcattagcat catatctgcc
     3661 atctcttgtt cgtgcatctt gatatcctta ctagtcatat tcaattgcat tagtgctcca
     3721 tcaatattat tatcctcaag atccataatg gcttccatta aatgcatttt agcattatcc
     3781 aaggaggaac caaaagtcat gttgcttccg ggtaacatca tactattatt tccattcact
     3841 aactcattgt catactgagt agtagttata ggatcttcag ttaaagctaa tgctttgaaa
     3901 gaaactgaat ttattaaatt taatgctaaa ccaaagatta ttaatgatat catttgaata
     3961 aatgtaaatt ctttttcttt catcatttaa gtacttttaa aaatattatt taaagaatac
     4021 tattaatttt aatatgcaat agcgatacat atcttaatta atacaataat ttattttttg
     4081 aatatgttat aaactatatt ataaacatta agattcactt atttgattac taaagtcttt
     4141 taaattaaaa attgttatta cacaatattg atactttaat ttatttaatt taattatttg
     4201 ataggttcaa aaagttgcat attttaaact aaaagttaaa agaacatatt atattacttt
     4261 tactttgata atattatgca tagaagagga tcaaattcat atttatccat tcaaacagca
     4321 tttatcaaat taaggaaaag tattattatt cagccaatgt ctgaagaatt agatactaaa
     4381 gaggcattag ggagagttct atataacaat attatttcat caatcaatat tccgatgtat
     4441 gattcttcac acatggatgg ttttgctgtt cttcacgaag acctaaaagg agcttcaaat
     4501 ttgaaaccaa taacactgaa agtaatagac aatatcaaac taggaaatac taaaataaag
     4561 cctttacaat cagggcatgc ttccagaatc cctactggag gatatttacc taaaaattct
     4621 gatactgttg ttccaattga atatgtacaa tttaatttat tcaataaatc ggttaaaatt
     4681 ttttctgaat ttcccaaacg atcatttata tctcctgccg gaatggatat ttctaaaaat
     4741 aaattattac ttaaaaaggg gcatttaatt cgtacccaag atatagcttt gttaaactta
     4801 cttagtatta aaaaactaaa cgtatttaaa aaaccgagag ttgccataat tcctactggt
     4861 aatgaattaa ccaataatat agatgaggtt attcaatatg gaaaagttct caatactaat
     4921 ggtcaaatta tttcctcatt aatagaagca tctggaggaa ttcctattga tttaggaata
     4981 gcatcggaca atattaaaga tatcaaaaaa aagatggaat atgccatatc tcaaaatgat
     5041 attattgtta caataggtgg atcctctgta ggacataaag accttattgc agaatctatc
     5101 gattctatgg gagaacctgg aattttcgca gaggggatta aactggatag gggaagagta
     5161 actaaaattg ctatattaaa atcaaaacct attatcatat tacctggacc tatacaagga
     5221 gctgttaatg ctttcattgt attggttatg ccattaatta ggtccttaat tggattatcg
     5281 cataataacc aagctattat caatgctcaa atttctgata attggaatgc aagaaagaaa
     5341 tttcaacatt ttgtaaaaat attgtatgtg catttatcaa tttcaaaagc taaccataag
     5401 ataaatgctg aaccaataat tggagaaact gccaatatga cagttatgac taaggcaaat
     5461 ggttatgtaa tgataccaga aaaaataaca agtattgaga agggaaatga aatacaagta
     5521 aatttacttt ctggattttc ttttgcgtct agaaatccca tagacctcat ttgatcaatt
     5581 atttgaataa tcttgtagaa agattaatag ctgctactgc aactaaaatt cctattatta
     5641 ttataatttt agtatgatca aatttgaatg ctcctagcat agatccaatt ataccaccag
     5701 taagtacaac aatagataag ggtattatta agtcaaaatt aacatctttg ctgttggtta
     5761 tagaatgagc aagaaatccg cttatagaat ttgttaatat gaataagcta gcagaagcag
     5821 ctgcctcttt gggattagct aaacctgaca ttatcaagat tggtgaaagc catattcctc
     5881 cacctattcc tacaagacca gaaatactgc ctagaagagc acctaaagga caagttatta
     5941 gtgtagtctt gataatattc aaattcattt ttttcatttt ttcatattga tgtgttattc
     6001 ttcttcttct tcttcttcca gacaatagca atgaggcaga agcaaaaaaa agagatatta
     6061 caaaaattag tgttaatatt ttttcgggta aaactataga accacctaga aatgcaaaag
     6121 gtacagaaat aaaaagtggg atggataatc ttaacgaaag atgacctgct ctcaaataat
     6181 taaaaaaagc tgctgaagat acaataatgt ttaatgccaa agaaattgga ggaacagaat
     6241 acaaattaac tccaacaaga actagtatag caatataact actccctcca gcaaatccga
     6301 cagaagaata aaaaaaactt attacaaaaa agataggagc aagtactaat agtagaatct
     6361 cattaatgac ctttccctcc caacatatta aatgcatgaa atatactagg taaaagaaga
     6421 tctaatgatt ctgatactgc tttagtgctt ccaggtaaat ttataattaa agttttacct
     6481 cttgttccag atattcccct tgagagcatt gataatggtg ttctcctttg accatatgat
     6541 cggatagctt cagaaattcc atttgtttct ttatcaatag tcattttagt agcctcagga
     6601 gttacatctc ttttactaaa accagtacct ccagaagtta ttattagatc tacttttttc
     6661 tcatcagaat atttgataat gtatgtctta attttgtcta tattatcagg taatacttca
     6721 tagcctataa tttcagtatt agtagcataa gtatttttta acctatcact gatcattttg
     6781 cctgatttgt caactcttgt accattaaaa gtagagtcac ttataacaaa aatagcaaca
     6841 gtaatttttt tatcaagatt tttttcaatg aaatctcttt taccaccaat tttatctaac
     6901 aattttattg attctattga aagatgttca tctattggtt tgagcatatc atatacagca
     6961 agtgcagcaa tactgcaacc agttaatgct tccatttcta ctcctgtttt ccatacactt
     7021 ttaacactga ctttaatttc tatgattgat tcgtataatg ataattgtgt agaaatatga
     7081 tctataggaa taggatgaca ataaggtatc aattcccatg ttttttttgc tccaagtgca
     7141 gctgaaattt ttgctgcctc aataatatct ccctttggca ttttttgttt ttttattaga
     7201 tctacagttt cagaacttag tttaattatt gcttgtgcaa tagcaaacct taaagtgtct
     7261 gctttattag atacatcaaa cattccttta ttgtagttat taattgttgt tttattcatt
     7321 tatcataact ccttccaccc attcagaatc cccagattcc attttttcct ttttccaaat
     7381 tggacatctt tttttaatat tgtcaattcc ataactacac gcttcaaatg cctcttttct
     7441 atgttctgat gaaactccaa ctataacact tatttctcca actttaagat atccaattct
     7501 atgtattgca ataaattttt taatattcca atttttaaat acctcatttt caattttttt
     7561 caatatctca ttgaccattt cctcatatgc ttcataaaag atgccctcga ctttttcctt
     7621 ggaattatga tccctaacaa ttccagtaaa aaaaacaact gcgccagaag aattatcttt
     7681 aagttcttct aaaattttta attgatcaat tgtatctttt attattgaaa tcatattatt
     7741 cctctttact atttttatcc tccacttatt ggtggtatta gagcaatttc atcagatgag
     7801 cttactttga cattttcgtt acatattact ttattaacag caaatacaaa gtttatctca
     7861 ttcatagaaa gatgaggatc caattcttta agacaatttt tgagttcttc taatgaaatt
     7921 gaattattat taataggaat actaagagta ttacttttca atttctctct taatattcca
     7981 aatactttta actttaatgt tctatcttca caggagttgt tcactttagt tattcaacca
     8041 cctattgtat gcatgagatt tagggttggt ctaagttgat taattcgaat tagtttgact
     8101 attccttctg gtttcatttt ataacaattt acaatatatt ctctaatttc ttgatcagta
     8161 atctttgcat ttctaatcat tttctttaag tcatatcctt ctttttcgaa aagacaagta
     8221 agaaatcttc catcagaagt caatcttatc ctatcgcaat ttgaacaaaa tggatctgta
     8281 attgatggga taaatccaat ttttcctttt ttgtcatcaa aattataaat agtagcagga
     8341 tcagatgtat aagaattatt tttatctaaa gaaataacat tatgaaaatt agtttctatt
     8401 atttttatta tttctttttt agtcataact agattataat tccagatacc ggatccgtca
     8461 aggggcataa attctataaa acgtacagtt atgtccatat ccctagcaaa ctttacaaag
     8521 ttaattattt catcatcatt ccaattacga attattacag tattaatttt tatttttact
     8581 cctaattctc ttgctttttg aatacctcga agaactttat ttaaattaga aatgccgttt
     8641 aaaactctaa atcgttcttc tctaaaagta tcaaggctaa tatttagaga attcaatcct
     8701 gctgatttta atttgtttat attctcttct aacaaaaaac cattagtagt catactaatg
     8761 gtttctatat tttctatctt tatcaaatcg ctaattaaat tctctaatga tggtctaagt
     8821 aatggttctc cgccagtaag tctaattttt gttattccta attgtgtaag aatggatact
     8881 attctaatta tttctttaaa attaagaatt tctttattct caatccattt aatgttacca
     8941 cttggcatac aataaatgca tttcatatta catttatcag ttacagaaat tcttaatttt
     9001 ctggctattc tattataact atctttcaaa tttttaggag gattagttga aattaaattg
     9061 aattttttca ccatataaat cgattattat ttctttggat gtgataattt ttcaagaaaa
     9121 tattgtttcc acccttgtgg aagatcttga actttaatgg ctttttccat aagtattgtg
     9181 ttctctttat cctcttttgt aacaagctct actatatttt ttattgtgac attattatcg
     9241 tctttcttaa ttagtttaat agtatcatta gtgcctattt ctccttcctt gataacttta
     9301 aagtaaattc cagacttctc actctctaaa aatttcttaa ttacatccat tctaccaaac
     9361 ttgataccaa gcttgtagca tggcatacgt ggttgagtta caactatttg tgaagaacct
     9421 atttgaaata tatctccaac atttacttta gattcaaaaa gaccttctgt tgttaaattt
     9481 tctccaaaca ttccccagtt aaatgagata cctggaaact cttttttcca aaaatcataa
     9541 tattcaaatg gataagaata tactgcctta tctggaccac catgtacagt cagatcagct
     9601 tgtctatcac cttcgagatt taatttcttt aaatttatcc ttttatcaac aggatttttg
     9661 aaaattccag tagtgactgt ttcttttcca aattttacta ttcttggtaa tccaacattt
     9721 acagaaacta ctttcactgt taattatatc aagtcctatc aaataagttt atattaatta
     9781 attagggtat aaattggact caatctggga aaatagtttt ttaattcaag ctacgaataa
     9841 cttctaatga caatcagaca ttcgtcaagt cacagatatg ggttatggat tttagcaata
     9901 ggaatagtta caatggtttt ttcaatctct ttattctcct cttcttctat tgctccctca
     9961 gtagtagtaa attcaaggaa ggttctaatt gggtagcatt acgaatgtgc ctcatatgtg
    10021 gacatatagg atgttgtgat tcttcacctg gaattcatgc aactaaacac cacgaaacta
    10081 ctggacatcc cattatgatt gcactgcctg atagatcatg gaaatggtat tatatacata
    10141 aacaataacg gataataacc cagttcaatt tgagaaagta ttgtagacca taagatctaa
    10201 ctacgaaagg tattcttata taatatattt tatataagaa aaatacttgg attaaatata
    10261 atatgggtaa tgacgtatta gtgaatcaca cctttacgca aattatttaa tttaattgtc
    10321 caattattta ttattataat agatataaaa aactttatta ttggaaaatt agagaaatta
    10381 catatagata aaagatgatt taatacaatt aagccagtta ctttttttta atatagtaaa
    10441 taataataat tgtaattcat aattatatca tattccgccc ctactatctg actttttaat
    10501 ctcaattgta actttgtctc ctactcttaa gccagtttca tcatattctc gtatacttaa
    10561 tttgaatgta ggtgaattac ccacatctac accactacca ctaaacatct ttggtaagtt
    10621 tttcattaaa tcttctggcg aggtaaaagc cataccagta cctccaaatg atggttgctg
    10681 ttgatttcct cttctaggag ttttagtatc attgaagaca acataaacaa aaggggtttt
    10741 ttcatcttga gacgcctcga tttttgatac tataaattca ctcttcataa ata
//
LOCUS       DATAFW010000067         4109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4787412, whole genome shotgun sequence.
ACCESSION   DATAFW010000067 DATAFW010000000
VERSION     DATAFW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4787412"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VHJ57_01630"
     CDS             complement(<1..464)
                     /locus_tag="VHJ57_01630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX2487266.1"
                     /translation="MKTIQKKTQNQAKNKNQRKKNIKSNNSRSKGKFSKVNNYVSKTA
                     NIGKNVKIWHFAYVGDNTIIGDNVMIGSLSHIDYNVKIGNNTRIEGAVYIPPLTVIGN
                     NVFIAPGATFTNDPYPMSPKMIGVTVEDGAIIAARAVIKPGVTIGKNAVVGMG"
     gene            complement(497..898)
                     /locus_tag="VHJ57_01635"
     CDS             complement(497..898)
                     /locus_tag="VHJ57_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX2487267.1"
                     /translation="MKKNMLDILACPIDKNYPLVLIELNVEELQKNKQTENSRTLNSD
                     GEHNNIVKQNNKDDGGNKVNEINHNEKVIVIIDGILYCKKCSRFYPIIDEIPIMLPDE
                     LREKEKDLQFLKKWKDSIPENILTNSNPWHL"
     gene            complement(905..1975)
                     /locus_tag="VHJ57_01640"
     CDS             complement(905..1975)
                     /locus_tag="VHJ57_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX2487268.1"
                     /translation="MVEDILSPAKDVSGVDNVIELGKYQDINAKSNLHKIGSKNGNVV
                     KIGIIGVGGWGKNHSRVLHDFGVLTSICDMDKTRAKDLATRFNVNYYGSMDEMINSES
                     LDACLVCTPTKTHYPVSKKLMEKGINVFVEKPLSYLSKECEEMIQIAKKNKVSLTCGY
                     IERFNPAVQEVKKILSEKIYGELLMMEFHRENRMPLHIKDVGIIYDTAVHDIDTAIYL
                     FGTNPHVVFARAGKIFHSFEDFSNIMLGFGTDRAAVISSNWITPKKVRNFSAVCTDAI
                     INGDFIKQEITIEDSLSTTTPRRINLVEPLSLELRNFLEVIEGKRDRPEVLPEEATNV
                     TKIAEAALLSSNTGAPIYLNLG"
     gene            complement(1969..2892)
                     /locus_tag="VHJ57_01645"
     CDS             complement(1969..2892)
                     /locus_tag="VHJ57_01645"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX2487269.1"
                     /translation="MKNKGAEYIWFDGKFIEWEKALIPINTHALHYGTSIFEGIRAYK
                     SNNNLHIFKLKEHMERLFRSASIYSIKINHTIDEMVDGTVQLLRKVNMRESCYIRPLS
                     FVGVHGIDLNVTYESPTHTFIIVFPFARYFQTEGIRVGVSSWRRIHDSSMPPIAKSGA
                     NYLNSILATQECKRNGYDEAIMLDKNGNVSEAPGENIFVIKNKTIFTPDMASSILEGV
                     TRDTVIELAKDLGYTIIQRPILRSELYIAEEIFLTGTAAEIIGVVSVDGRNIGNGKEG
                     QITKKIRNTYNEIVTCNNNNEKYKGWTTEVW"
     gene            complement(2943..3569)
                     /locus_tag="VHJ57_01650"
     CDS             complement(2943..3569)
                     /locus_tag="VHJ57_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487270.1"
                     /translation="MEKLAALYLAHLNPFTKAHQEIISSLQKKYTVYVYPVRFLKNGK
                     ELNTRSFPFPYEVRKLMIESVFPNEKNVIVSPNYTFFAPFIKYLPPIFSPYSWKIREQ
                     VLKSVREKKFISYTGDRVERYALMIYRFNPIKAKRLPISATQVKEDIYEQALSKKRDL
                     INSQWKDKIPKPVIKIIEDNWDIVEKFANKNDETLKVVGIKFPKKGFF"
     gene            complement(3708..4034)
                     /locus_tag="VHJ57_01655"
     CDS             complement(3708..4034)
                     /locus_tag="VHJ57_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEX2487271.1"
                     /translation="MSEETQQQNQITPQPNDFQIRDAIYIGKKPLMAYVTSTLIQLAN
                     QPTVTIKARGLSIGRAVDVSQIILKRMENAGYTIGDVKIGSETVQSEDGRTRNVSTIE
                     IVIKRS"
BASE COUNT         1236 a          713 c          567 g         1593 t
ORIGIN      
        1 cccataccaa caactgcatt ctttccaata gttacaccag gttttattac tgctctagcc
       61 gctattattg ctccatcctc tacagtaact cctatcattt ttggactcat gggataagga
      121 tcattggtga acgttgcacc aggtgcaatg aatacattgt ttcctattac tgttaatggt
      181 ggaatatata cagccccctc aatacgggta ttatttccaa ttttaacatt ataatcaata
      241 tgtgaaagag atccaatcat aacattgtca cctattatgg tattatctcc gacatatgca
      301 aaatgccata tctttacatt ttttccaata tttgctgtct ttgatacata attgtttact
      361 tttgaaaatt tacctttgct gcgactatta ttacttttaa tatttttttt acgttggttt
      421 ttatttttag cctgattttg cgtttttttt tgaatagttt tcatttttat ttagggatat
      481 atttgttatg tataacctat aaatgccacg gattagaatt tgttaaaata ttctcaggga
      541 tactatcttt ccattttttt aaaaattgca gatctttttc tttttctcgt agttcatcag
      601 gtaacatgat gggaatttca tcaataatag gataaaaacg agaacatttt ttacaatata
      661 gtattccatc aattataaca ataactttct cattgtgatt aatttcattt actttgtttc
      721 ctccatcatc tttgttgttt tgtttgacaa tattattgtg ctcaccatca ctattcagtg
      781 tacggctatt ctctgtttgc ttgttttttt gtaattcttc cacatttaac tctattaaaa
      841 ccaaaggata gtttttatct atgggacaag ccagtatgtc cagcatattt tttttcattt
      901 tatatcatcc taaattaaga taaatgggag ctcccgtatt actcgataaa agtgctgctt
      961 ctgcaatctt ggttacgtta gtagcttctt ctggtaatac ttctgggcga tctcttttcc
     1021 cttcaataac ttctagaaaa tttcttaatt ccagtgaaag aggttcaact aaatttattc
     1081 ttctaggagt agttgtagaa agagaatctt ctatagttat ttcctgtttg ataaaatcac
     1141 cattgataat tgcatcagta caaactgcgc taaagtttct aacctttttt ggtgtaatcc
     1201 aatttgacga aattactgct gctctatctg ttccaaagcc tagcatgata ttagaaaaat
     1261 cttcaaagga atgaaaaatc tttcctgctc ttgcaaatac aacatgaggg ttagtcccaa
     1321 aaagatatat cgcagtatct atatcatgaa ctgccgtatc atagataatt cccacatcct
     1381 tgatatgtaa cggcattcta ttttctcgat gaaattccat catcagtaac tctccataaa
     1441 ttttttcaga tagtattttt tttacctctt gaacagctgg attaaacctt tcaatatatc
     1501 cacatgtcag tgaaacttta tttttttttg ctatttgtat catttcttcg cattccttag
     1561 ataagtagga taaaggtttt tcaacaaata cattaattcc cttttccatc agctttttgc
     1621 taactggata atgtgtcttt gttggagtac aaactaggca tgcatctaaa ctttcggaat
     1681 tgatcatttc atccattgaa ccataataat ttacgttaaa tctagttgcc agatctttag
     1741 cacgagtttt atccatatcg catattgatg ttaatacacc aaaatcatga agaacccttg
     1801 aatgattttt tccccatccg ccaacaccta tgataccaat ttttacaaca ttaccattct
     1861 ttgatccaat tttatgtaga tttgatttgg cattgatatc ttgatatttt cctaattcta
     1921 ttacattatc gactccactt acatctttag caggagagag aatatcttct accatacctc
     1981 agtagtccat cctttatatt tctcattatt gttgttacag gtaactattt cattgtatgt
     2041 attccttatt tttttagtta tttgaccttc ttttccattt ccaatatttc taccatcaac
     2101 actaacaacg ccgatgatct cagcagcagt tcctgtcaaa aaaatctctt cagcaatata
     2161 aagttctgaa cgtaatattg gtcgttgaat aatagtgtaa ccaagatctt ttgcaagttc
     2221 aattactgtg tctcttgtaa ctccttctaa aattgatgaa gccatatcag gagtaaatat
     2281 agttttattc ttaataacaa aaatattttc cccaggagcc tcacttacat ttccgttttt
     2341 gtctagcatg atagcttcat cataaccatt tcttttacat tcttgtgttg ccaaaatgga
     2401 attaagataa ttagctccag acttagcaat gggaggcata gaagaatcat gaattcttct
     2461 ccatgaggaa acacctactc taataccttc tgtttgaaag tatcttgcaa aaggaaatac
     2521 aataataaac gtatgagttg gagattcata agttacattt agatctatac cgtgaactcc
     2581 tacaaatgaa agtggcctaa tataacagga ttctctcatg tttacttttc ttaatagctg
     2641 tactgttcca tctaccattt catctatggt atgattaatt ttaatagaat atatggacgc
     2701 ggagcgaaat agtctttcca tgtgttcttt gagtttaaaa atatgtagat tgttgtttga
     2761 tttatacgct ctaattcctt cgaagatcga agtcccataa tgtagtgcat gtgtatttat
     2821 cggaattaac gctttctccc attcaataaa tttaccgtca aaccaaatat attctgcgcc
     2881 cttgttcttc attttaagaa acgtatttta agaattaata aaacttttct atagatattt
     2941 aattaaaaaa atcctttttt agggaatttt attccaacta cttttaaagt ttcatcattt
     3001 ttatttgcaa atttttcaac tatatcccaa ttatcttcta taattttgat taccggcttt
     3061 ggaattttat ctttccattg tgaatttatt aaatctcttt ttttacttag agcctgttca
     3121 taaatatctt ctttaacctg agtggctgaa atgggtagtc ttttggcttt aattggatta
     3181 aatctataaa tcattagagc ataccgttct actcggtcac ctgtatagga tataaatttt
     3241 ttttctctaa ctgactttaa gacctgttcc ctaattttcc aggaataagg agaaaaaatt
     3301 ggcggtaggt acttgataaa aggagcaaaa aatgtataat taggtgaaac aataacgttt
     3361 ttttcatttg gaaaaacaga ttctatcatt aattttctaa cctcatatgg aaatggaaag
     3421 ctcctggtat tcagctcctt tccatttttt aaaaaccgaa ctggatagac atatacggta
     3481 tattttttct gaagactaga tattatctct tgatgtgctt ttgtaaatgg attaagatgt
     3541 gctaaataaa gtgcagcaag tttttccata agccaactat tactaaatct tgattttggt
     3601 ctttctgatg taaatttaag attttagtaa ttattataat aatagtagta ataatataaa
     3661 aataacaaca aaaattaatg ttagttttca aaaaaataac taactaacta gcttcttttg
     3721 attactattt cgattgtaga gacgtttctt gttcttccat cttctgattg tacagtctct
     3781 gaaccgatct ttacatcacc aatagtgtag cctgcattct ccattctttt caatataatt
     3841 tgtgaaacat caacggctct gccgatactt aatcctctcg ctttaatggt tacagtgggc
     3901 tgatttgcta attgaattaa agttgaggta acatatgcca tcaatggttt ttttccaata
     3961 tatattgcat ctcgtatttg aaaatcgttt ggttgtggtg taatctgatt ttgttgttgt
     4021 gtttcttcag acatactact gtttcttgct aaggataatt taaatctaaa agttcattat
     4081 tgaataaaga atcaatcaaa atattaaat
//
LOCUS       DATAFW010000068         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6198204, whole genome shotgun sequence.
ACCESSION   DATAFW010000068 DATAFW010000000
VERSION     DATAFW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6198204"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..2184
                     /locus_tag="VHJ57_01660"
     CDS             52..2184
                     /locus_tag="VHJ57_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX2487272.1"
                     /translation="MMINKDNKPNKLIQESSPYLLQHAYNPVEWFPWGDEALHKAKKE
                     NKPIFLSIGYSACHWCHVMAHESFEDENIARILNAKFINIKVDREERPDIDDIYQKAC
                     QLAMGNGGWPLSVFLTPDQKPFYVGTYFPKQSRYGLPGFSTILETLSQAYHDKKEDID
                     KATHELMESLLESSFTLTKNTNTEIEKTILDESAINLLQIADPLNGGFGQAPKFPNTT
                     NLLLLLRYFDYSKNSQFLNFVEFTVKKMAWGGIHDHIGGGFSRYSTDEKWLIPHFEKM
                     LYDNALLIQLFSELYQATGKNQYLTLIQTTIQYLLRDMNLLKEKNEYTAFYSSQDADS
                     EGIEGKFYTWTKDEIKENIKDTQKFELFCDYYGITQGGNFEGTNILNITKSKETLSKQ
                     CNIAIDKVDDILNETLEILYKVRKKRIAPGKDEKILTSWNALMISALVSAYKVTDNKD
                     YLDFAKKTINFIETNLSYDHNRLKHTYKDGFSKINGYLDDYSYYINALLDVFEISSNP
                     FYLQRALDYMEALVSHFWDDNAKSFFFTSDDQEQLIIRTKNFYDLAIPSGNSMAVYAL
                     LRIQYMTQNQDYLDKAESIIKSCYKLALGNPFGFGQLLISIYLYFKKPVEIIILQNKK
                     NDYNKREMKKWIMKQYIPNGITFTLEYPSNELQQLMSSNIFSLLKGKKINIADSEKFR
                     ECVYICKNFTCSLPMYSVAELKKYLNSN"
     gene            complement(2331..2489)
                     /locus_tag="VHJ57_01665"
     CDS             complement(2331..2489)
                     /locus_tag="VHJ57_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487273.1"
                     /translation="MCAKEEFKNWFEKKNIVKKKNPNIKQSKKVILIGVAVYGKMHRD
                     AEISGLLS"
     gene            2976..>3301
                     /locus_tag="VHJ57_01670"
     CDS             2976..>3301
                     /locus_tag="VHJ57_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487274.1"
                     /translation="MNDSPRIPFDCGVTIEDQLYLAYEKKIDALFITNHNTLNGYKQM
                     LAYRDNHEKLKSIKIYPAEEITINTKGHVLAYGIYETIKPGMDIDETLDAIKKQNAIS
                     CAAHPFA"
BASE COUNT         1247 a          452 c          488 g         1114 t
ORIGIN      
        1 gtagaaatca taataaatac aattaaaaga tcgtaatata atatttaaca aatgatgata
       61 aataaagata ataaacctaa taaattaatt caggagtcta gtccttatct tttacaacat
      121 gcatataatc ctgttgaatg gtttccatgg ggagatgagg cattacataa agctaaaaaa
      181 gaaaataaac ctatctttct tagtattgga tacagtgctt gccactggtg tcatgttatg
      241 gcacatgaat cttttgaaga tgaaaatatt gccagaattc tgaatgcaaa atttattaat
      301 attaaagtgg atagagaaga aagaccagac attgatgata tttaccagaa agcatgtcaa
      361 ctagctatgg gcaatggtgg ttggcctttg tccgtatttc tgactcctga tcagaagcca
      421 ttctatgtag gaacttattt ccctaaacaa tctagatatg gacttccagg attttcgaca
      481 attttagaaa cattatctca agcttatcat gataaaaagg aagatattga taaagcaaca
      541 catgaactta tggaatccct tctagaatca tcatttactc ttactaagaa tactaatact
      601 gaaatagaaa aaacaatttt agatgaatcc gcgataaacc ttctacaaat tgcagatcct
      661 ttaaatggag gatttggcca agctcctaaa ttcccgaata ctactaatct tttgttgtta
      721 ctcagatatt ttgactactc caaaaatagc caatttctta acttcgtaga atttacagtt
      781 aaaaaaatgg cttggggcgg aattcatgat catatagggg gaggtttttc aagatattct
      841 acagacgaaa aatggcttat tcctcatttt gaaaaaatgc tctatgataa tgcgttgtta
      901 attcaattgt tctctgagct ttatcaagct acagggaaga accagtattt gacattaatc
      961 caaacaacaa ttcaatattt gctaagagac atgaacctct tgaaggaaaa aaatgaatat
     1021 acagcattct attcatcaca agatgcagat tctgaaggaa ttgaaggaaa attttatact
     1081 tggacaaagg acgaaattaa agaaaatata aaagatactc agaaatttga attgttctgt
     1141 gattattatg ggataactca aggcggtaat tttgaaggaa caaatatttt aaatattaca
     1201 aagtcaaaag aaactttatc aaaacaatgc aatatcgcaa tagataaggt cgacgatata
     1261 ctgaatgaaa ccttggagat tttatataag gttagaaaaa aaaggattgc tccaggcaaa
     1321 gatgaaaaga ttttaacttc atggaatgcg ttgatgattt ctgctttggt ttcagcatat
     1381 aaagttactg ataataaaga ttatttagat tttgcaaaaa aaacaataaa ttttattgaa
     1441 acaaatttat catatgatca taatagatta aaacatacct ataaagacgg attttcaaaa
     1501 attaatggat atttggatga ttattcttat tatattaatg cacttttgga tgtttttgaa
     1561 ataagctcta atccatttta tcttcaaaga gcccttgatt atatggaagc actagtaagc
     1621 catttttggg atgacaatgc aaaaagtttc ttttttacat cagacgatca agaacaattg
     1681 atcataagaa caaaaaattt ttatgattta gcaataccta gtggaaattc tatggcagtt
     1741 tatgcattat tgcgtattca atatatgact caaaaccaag actacctaga taaggccgaa
     1801 tcaattatca aatcttgtta taaactagcg ttaggaaatc cttttggatt tggtcaatta
     1861 ttgatttcaa tttatttgta ttttaaaaaa ccagtagaga taattatatt acaaaataaa
     1921 aaaaatgact acaacaaaag agaaatgaaa aaatggatta tgaaacaata tattccaaat
     1981 ggtataacat tcactttaga atatccatct aatgaattac agcagttaat gtcatcaaat
     2041 attttttctc ttttaaaagg aaaaaaaata aacattgcag atagtgagaa atttagagaa
     2101 tgtgtctata tctgtaaaaa ctttacatgt tcattaccga tgtattcagt ggccgaatta
     2161 aaaaaatatc taaatagtaa ttgagattta tcatgtaatt acatatctaa ctaaatcata
     2221 caatatttta tcaatatttt gttgcaagta caggactaat tgcgtatgtt actatatctg
     2281 atactgggtt tgtaatagtc attttttaat tccctatttt ctttttaacc ctaagatagt
     2341 aacccagata tttcagcatc gcgatgcatc ttcccataca ccgctacccc tattaatata
     2401 actttcttag attgtttgat atttggattc ttttttttaa ctatattttt tttctcaaac
     2461 caatttttaa actcttcttt tgcacacaat gattacatca atttgtctaa gaaaaacaag
     2521 ctatggtaaa ttaatatatt catattggga atgagaacta ctacgtagaa aagattattt
     2581 ctctaaaatt tatcagtcat agtactagca atgttgacat tatgaaatga tgattttgat
     2641 acatctatat tgatcagtat ttttaaaaat atcattatta actatataaa taaagatgta
     2701 ctaatttaaa taaatctatt attttaaatc ataagatcaa aaataataat agtatcttat
     2761 gatattaatc tagcttgcag tctttgggtt tattgtgtta gttaggagag aaaaatcttg
     2821 ttaatattga ataaacctcc gcgttattaa gctactattc gatatagatt tatagatagt
     2881 catatataag agaaattacc catttaattg ggtttataga tacggctcaa ttattaaata
     2941 tggaattaca ctgtcataat gttttttcta attatatgaa tgactctcct aggattcctt
     3001 ttgattgtgg tgttaccatt gaagatcagc tgtatttagc atatgaaaaa aagattgatg
     3061 ctttgtttat taccaatcat aatacattga acggatataa gcaaatgtta gcatatagag
     3121 ataatcatga aaaattaaaa agtattaaaa tatatcctgc agaagagata accatcaata
     3181 caaaaggtca tgttttagcc tatggaatat atgaaactat caaaccaggt atggatatag
     3241 atgaaacgtt agacgctata aaaaaacaaa atgctatttc ttgtgcagcc catccttttg
     3301 c
//
LOCUS       DATAFW010000069         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4436696, whole genome shotgun sequence.
ACCESSION   DATAFW010000069 DATAFW010000000
VERSION     DATAFW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4436696"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..712
                     /locus_tag="VHJ57_01675"
     CDS             86..712
                     /locus_tag="VHJ57_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX2487275.1"
                     /translation="MSNPTDILNKLFKETKPQDRLYPIPETPIQDFDSEDILNTNIYN
                     VMLNRRSQRKFEIKEVEDWKLDIIFAAADTAPTAGGFQGFEIFHIKNPDIKIKLVVAA
                     NNQPYVNAPVVLVFCMNPRRVKMQFSSEILRKFSIQDATLAAAYAQLAAHSLGLSSIW
                     IGMIDDRKVMETIGTDLLPSSILCIGYPKKVLQPKPKRALSELIHTIE"
     gene            complement(800..1219)
                     /locus_tag="VHJ57_01680"
     CDS             complement(800..1219)
                     /locus_tag="VHJ57_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HEX2487276.1"
                     /translation="MTENIRKTTFPHLQIQIEVYCKVNPTESEDVISKAINLILEGIT
                     LEFKYGDRIVGQSGNLKVLSTIYNQVRDRSTTSVLRRILLSHLDQSSTWFYINKQVST
                     MGIIVLVDDEFESPLGPIRIYLKSNDIDRVIEWLCPE"
     gene            complement(1223..1798)
                     /locus_tag="VHJ57_01685"
     CDS             complement(1223..1798)
                     /locus_tag="VHJ57_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487277.1"
                     /translation="MKQPFIICLTGMPGAGKSTVADAIKDEGFILITMGDCIRMEANR
                     KNLQLTDKNLGTLMLDLRNMYGPGAIAQLVINQIQKKYYDPKYNKFIIDGIRSIHEVE
                     LMKTIGIVKILAIHGSVITRFLHLKNRSRSDAPSSLEDCIMRDRRELSVGISEAIAFA
                     DEMISNNEITVEQLKKKAKSIAKKWIYSLES"
BASE COUNT          824 a          362 c          319 g          832 t
ORIGIN      
        1 gtaaaattaa attgtaatat taggaaggat aaaatataga taaatttccg aaaaagtttt
       61 ctaatgatta aaagcttgta atattatgtc aaatccaaca gatatactaa ataagttatt
      121 taaggaaact aaaccccaag atagactata tcctataccc gaaaccccca tccaagactt
      181 tgattctgaa gatattttaa acactaacat ttataatgtg atgttaaata gacgttctca
      241 aagaaaattt gagattaaag aggtggaaga ctggaaacta gatataattt ttgctgctgc
      301 agacactgct cctactgctg gaggttttca aggatttgag atttttcata ttaaaaatcc
      361 tgacataaaa ataaaactag ttgtcgcagc aaataatcag ccctatgtaa atgctcctgt
      421 agtattagta ttctgtatga acccacgtag agtgaaaatg caattctcat ccgaaatttt
      481 aagaaaattt tctatacaag atgcaacact tgctgctgct tatgctcagt tagctgctca
      541 ttcgctcggt cttagttcta tatggattgg gatgattgac gacaggaaag tcatggaaac
      601 cataggtaca gatcttcttc catcttctat tttatgtatt ggttatccaa aaaaggtttt
      661 acaacccaaa ccaaaaagag cattatcaga actaatacat acaatagaat aatttgttga
      721 aataatgata atatccatat gtagatattt ttgttaaaat aacagataag gagattaaaa
      781 ttatgaatat gaattattct tattctgggc ataaccattc tataactcta tcaatatcat
      841 tacttttgag atatattcga attggtccta aaggtgattc gaattcatca tcaaccaata
      901 caataattcc catagttgaa acttgcttgt taatatagaa ccatgtagaa gattggtcta
      961 aatgagaaag taatatccta cgaagcacag aggttgttga cctgtcacga acttggttat
     1021 agatagtgct tagcactttt aagttaccac tttgacctac tatccgatct ccatatttaa
     1081 attctaaagt tataccttcc aaaataagat taattgcctt gcttattaca tcctcagatt
     1141 ctgtaggatt aactttacaa tatacctcta tttggatttg taaatgagga aatgttgttt
     1201 ttctaatatt ttctgtaatg acttaactct ctaatgaata tatccatttt tttgctatgg
     1261 atttagcttt tttctttaac tgctctactg ttatttcgtt attagatatc atttcatctg
     1321 cgaacgctat tgcttcactg attccaaccg ataattcccg tctatctctc attatacaat
     1381 cttcaagaga agaaggagca tcagatctag aacgattttt taaatgtaaa aatctagtaa
     1441 tgactgatcc atgtattgct aaaattttca ctattcctat tgtcttcatt aattcgactt
     1501 catggatact ccttatcccg tcaattataa atttattata tttgggatca taatattttt
     1561 tctgtatttg attaattact agttgagcaa ttgcacctgg accatacata tttcgtaaat
     1621 ctaacattag agttcccaag tttttatcag ttaattgtaa attttttcta ttggcttcca
     1681 ttctaataca atctcccatt gttatcagaa taaaaccttc atcttttatt gcatcagcta
     1741 cggtagattt ccctgcacct ggcattccag ttaaacagat tataaagggt tgtttgatat
     1801 ataattcacc taattatttt aatggaatgt aagcatatta tcaacttttt cttcctagta
     1861 atattataat ttaaatggtg attatatcca aaaactagca atcacaaaat cttgtagtat
     1921 taagcttcac ttattaaaat cgaaattatg taatctcaat ttttttgttt tttttgactc
     1981 attgattatt acataatttt tacccaattc acaatatatt atttctatag taaaaaatgg
     2041 taagaaaaga gaagaatata acattcaaaa aagtcaggaa catagtgggt gatctatttc
     2101 attggcttaa tttattaaat cataaataga tgtgcaaaat tagagtcaac tataaagaat
     2161 aatcataaag agttaattca gaaaaaaatg attaatatct aagaactgtg ttcttaaatc
     2221 aatataggtt attgatcact aatgatgaat ccagtagtga aaaagaaaaa tacaaataac
     2281 ttaattattc tatttgaatt tatgccaata actgatgcta ctaaaaaaca aattgca
//
LOCUS       DATAFW010000070         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_560652, whole genome shotgun sequence.
ACCESSION   DATAFW010000070 DATAFW010000000
VERSION     DATAFW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560652"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1221)
                     /gene="dph2"
                     /locus_tag="VHJ57_01690"
     CDS             complement(217..1221)
                     /gene="dph2"
                     /locus_tag="VHJ57_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HEX2487278.1"
                     /translation="MISIDEVRIFEVIEKRKPLSVALNGPEALFPKMQETGNKIMKLF
                     DIPAYIIGDTCWGSCDINTHAADMLGADLLFNIGHTISMETIDKKVIMINAYDDVNFD
                     IVVEKYANDRANQKYKILGIITDSQHLHQLQPSRKILENHGFKTVIGDGKGQLNDGQV
                     FGCEFYPAYDIQNRVDAYIFLGQSMFHSVSIAMSTEKPTFMLDPYFNEFQQVNDKARV
                     MEKKTILSIYKAQEATKIGIIIGLKEGQFSKIKALKFKKSLEVLGKDIQLIALTEITE
                     EKLRNFKGIDAYIQVACPRIAIDNHFTKPVLSVPQAEALIKVLKNEPMDDFLKLRHWL
                     "
     gene            1337..2191
                     /gene="folP"
                     /locus_tag="VHJ57_01695"
     CDS             1337..2191
                     /gene="folP"
                     /locus_tag="VHJ57_01695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX2487279.1"
                     /translation="MTKIGDVSINKNSVKIMGIINVSPESFYKNSIKTNVNSISTYVK
                     KLENDGADLIDIGAMSTAPYLNTLISSKIEISRLKKAIKIIRHSCKLPISVDTPRARV
                     AEEALKMGADAINDVCGLKYDPEMGEIVSKYNIPVILGAFEKKTNKILTAKSLSTKRI
                     LYDSITIAQKAGIRKTNIIIDPSIGFFRKEGNNPFFTRMTKTPWYCRDIEIVYNLKEL
                     TKLPFPICISLSNKSFLGKLMNLRINERLIPSLVMEIIAVINGATVIRTHNVKETSLA
                     LRTLQLVN"
     gene            2319..>2449
                     /locus_tag="VHJ57_01700"
     CDS             2319..>2449
                     /locus_tag="VHJ57_01700"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX2487280.1"
                     /translation="MNIREGLTFDDVLLIPKRSPIISRSQTNLKTRLSRNINLNIPV"
BASE COUNT          829 a          391 c          372 g          857 t
ORIGIN      
        1 ggtattgact gttaattctc gaaatgggtg aggtccggga gggagcaacc ctaaggtgac
       61 ataaccgtgc tggtccgtct acgtggtgga tcatatgaga tgcgagattg ggatttttat
      121 caaaggtggt actagtaggc tgccttcgca tttatgatat tgctctcgaa tactgaataa
      181 taatccttgc attatatagg tagcaatttg ataccttcat aaccaatgac gtagctttaa
      241 aaaatcatcc ataggttcgt tttttaaaac cttaattaat gcttcagctt gggggacaga
      301 aagtactggt ttagtaaaat gattgtctat agctattctg ggacaagcta cttgaatata
      361 agcatctatt cctttaaaat ttcttaattt ttcttcagta atttctgtta aagctattaa
      421 ttgtatatct tttcccaaga cttccagaga cttcttaaat tttaaggctt ttatctttga
      481 gaattggcct tcttttaatc ctattattat tccaattttg gttgcttctt gtgctttata
      541 aattgataaa atcgttttct tttccatgac tctagcctta tcattcactt gttgaaattc
      601 attaaagtat ggatccaaca tgaaagttgg cttttctgtt gacattgcaa tacttacaga
      661 gtgaaacata ctttggccta agaaaatata tgcatctact ctattttgta tatcataagc
      721 tggataaaat tcacagccaa aaacctgacc atcatttagc tgtcctttac catcaccaat
      781 tactgtttta aatccatgat tttctaatat ttttctagac ggttgtaatt gatgtaagtg
      841 ttgactatct gtaattatac ctaatatttt atatttttga ttggccctat cattagcata
      901 tttttctact acaatatcga agtttacatc atcgtatgca ttgatcataa ttactttttt
      961 atcaatagtt tccatagaaa ttgtatgtcc aatattaaac aacagatctg cacctagcat
     1021 atctgcagcg tgtgtattaa tatcgcaaga tccccaacaa gtatcaccta taatataggc
     1081 tggaatatca aacagtttca taatcttatt tccagtttct tgcatcttgg ggaataatgc
     1141 ttccggacca ttcagcgcta cggataaagg ttttctcttt tctattactt caaaaattct
     1201 tacttcatca attgaaatca atcaataatc ccattaaaat taatatatct ctcccatatt
     1261 atttttcata tcactatcaa aatgttagtc tattttttta gaaggaaata atatagagga
     1321 tacttatatt tgagtcttga caaaaattgg ggatgtttct ataaataaga attctgtcaa
     1381 aataatgggc ataattaatg ttagtcctga atctttttat aaaaattcaa taaaaactaa
     1441 tgtaaatagt atttctactt atgttaaaaa gttagaaaat gatggagcag atttaataga
     1501 tataggggca atgtcaacgg ctccatacct aaacacactg atttcttcaa aaattgaaat
     1561 ttcgagatta aaaaaagcta tcaagataat acggcacagt tgtaaacttc ccatatctgt
     1621 agatactcct agagccagag tagctgaaga ggcactcaag atgggagcag atgcgataaa
     1681 tgatgtatgt ggattaaaat atgatccgga aatgggcgaa attgtatcaa aatataacat
     1741 tccagtgatt ctaggtgcat ttgaaaaaaa aaccaataaa attctaacag ctaaatcttt
     1801 atcaacgaaa aggattcttt atgatagtat aacgattgca caaaaagctg gtattaggaa
     1861 aaccaatatt ataattgatc catccatagg tttttttaga aaagaaggga ataatccatt
     1921 ctttacaaga atgacaaaaa ctccatggta ttgtcgagat atagaaattg tctataatct
     1981 caaagaactc acaaagctcc cttttccaat ttgtatttct ctttcaaaca aatctttttt
     2041 aggaaaactt atgaatctca ggataaacga aagacttatc ccatctttag taatggagat
     2101 tattgctgtt attaacggtg caacggttat tcgtactcac aacgttaaag aaacatcatt
     2161 agcattacgt actttacaat tggtaaatta gtatttgtta tttaaattgc tttttgggtc
     2221 atgaaaagaa aatttgttca aatttaaaac ttcggctaat aaagatgaga gtaaggcaaa
     2281 acttataatt cttccttaac ttaataagaa aatgaatatt gaatattaga gaaggtctaa
     2341 cttttgacga tgtattatta atcccaaaac gttctccaat tatttctcga tctcaaacta
     2401 atttgaaaac cagattatca agaaacatca atttgaatat tcctgttat
//
LOCUS       DATAFW010000071         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_209056, whole genome shotgun sequence.
ACCESSION   DATAFW010000071 DATAFW010000000
VERSION     DATAFW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209056"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VHJ57_01705"
     CDS             <1..847
                     /locus_tag="VHJ57_01705"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEX2487281.1"
                     /translation="SGFLGISQQVFNRLYNDGLGAIVSKSISVSPLDGYKGPTVVSLG
                     EKGYLNAVGLANPGSDAFAHEIMKNQIPLIVSIVGSSQSDFPKLIRKFDKLNILGYEI
                     NLSCPHVAKMGMEVGDDPDLVTKIIKTLKSKTKKPVIIKVGIGNIDVLKLAMIIKESG
                     ADAITAINTIRAMAINVETGMPILSNKVGGLSGIPLKSIGVRCVYEISKKIDIPVIGC
                     GGIFTWEDAIEYFLAGATAIELGSVIGYTGLKAFNQIKLGITEYLQKKDCKNIKEIVG
                     LAHKY"
     gene            831..1652
                     /locus_tag="VHJ57_01710"
     CDS             831..1652
                     /locus_tag="VHJ57_01710"
                     /inference="COORDINATES: protein motif:HMM:NF000796.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEX2487282.1"
                     /translation="MHINIDKIRMVVIEEVIEETPSVKTFVFDDMLSSNAKPGQFLMV
                     WIPRAEELPMSLMISKKKNHASITIRKHGFGSTSLFEKKQNDLIGVRGPYGNYFSLSK
                     NVKRPLLVGGGTGLVPLMRLTTLFSKMQEKCTIIMGAQTHKEVLFKGLAEDILSKIRT
                     NIIVTTEDGSYGIKGVVTDALKYILKKEKFDMIYTCGPELMMVGIYNLSVAHSIPIQA
                     SLERYMKCGIGICASCCINDKLVCKDGTVFNENQLSEMSEFGKVFRDKSGRPSYY"
     gene            complement(1700..>1933)
                     /locus_tag="VHJ57_01715"
     CDS             complement(1700..>1933)
                     /locus_tag="VHJ57_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487283.1"
                     /translation="HLEPEVIGKVGKKGKPLLNIKDMDIDKYILRRSKISKKIRSFRA
                     KGFENKEKDLRKLSTSTPSFSSSSSASSSLHLL"
BASE COUNT          675 a          246 c          385 g          627 t
ORIGIN      
        1 atcaggattt ttaggcattt ctcagcaagt ttttaacaga ttatacaatg atggtttagg
       61 ggctatagtc agcaaatcaa taagtgttag tcctctagat ggatataaag gtcccacagt
      121 tgtttctctt ggagaaaaag gttatttaaa tgctgtaggg cttgctaatc ctggatctga
      181 tgcatttgct catgaaatta tgaagaatca gattcctctt atcgttagca ttgttggatc
      241 tagtcagtca gattttccaa aactaataag gaaatttgat aaattgaata tcttaggata
      301 tgagataaat ctttcttgtc cacatgttgc aaagatgggc atggaagttg gtgatgaccc
      361 tgatttggta actaaaataa ttaagacttt aaagtcaaaa actaaaaaac ctgtaattat
      421 caaagttgga ataggaaata tcgatgtttt aaaacttgcg atgataataa aagaatcagg
      481 agctgatgcc attactgcaa taaatactat tagagcaatg gcgataaatg tagaaactgg
      541 aatgcccatt ctgagcaata aagtgggtgg attgtctggt attcctttaa aatccatagg
      601 agtaagatgt gtttatgaaa tatctaaaaa aatcgatatt cctgtaattg gatgtggagg
      661 aatattcaca tgggaagatg caatagaata ctttttggca ggtgcaacag ctatagaatt
      721 aggatctgtc atcggatata caggtttgaa agcattcaat caaataaaat taggaattac
      781 agaatatttg cagaagaaag attgtaagaa cataaaggag attgttggtc ttgcacataa
      841 atattgataa aatacgaatg gttgttattg aagaggtaat agaggaaact ccttcggtaa
      901 agacttttgt ttttgatgat atgttatctt caaatgcaaa acctggacaa tttttaatgg
      961 tgtggatacc aagggcagaa gaacttccaa tgagtctgat gatctcaaaa aagaaaaatc
     1021 atgcttctat cacaatacgt aaacatggat ttggatccac ttctcttttt gaaaaaaaac
     1081 aaaatgatct aattggagta cgtgggcctt atggcaatta ttttagttta agtaagaatg
     1141 taaaacgtcc tttgttagta ggaggtggaa ctggtttggt tcccttgatg cgtctcacta
     1201 ctttatttag taaaatgcaa gagaaatgta caataattat gggtgcacaa acacacaaag
     1261 aggtactttt caaaggtctc gcagaagata tattaagtaa aataagaact aatattattg
     1321 ttaccacgga agacggtagt tatggaataa aaggagtggt aacagatgct ttaaaatata
     1381 ttttgaagaa agaaaaattt gatatgatct atacctgtgg tcccgaactt atgatggtgg
     1441 gcatttataa tttatcagtt gctcattcca ttccaattca agctagcctg gaaagataca
     1501 tgaaatgtgg aatagggatt tgtgcaagtt gttgtataaa tgacaaactt gtttgcaaag
     1561 atggtactgt atttaacgag aatcaattat ctgaaatgtc agaatttgga aaagttttca
     1621 gagataaatc tggacgtcca tcgtattatt aattaaatta ataattaata agttaaacaa
     1681 gttttttcag aatttattgc tataataagt gaagggagga ggaggcagaa gaagaagaag
     1741 aaaaagaagg agtggatgtt gataactttc tgaggtcttt ttctttattt tcaaatcctt
     1801 tagctctaaa cgacctaatt tttttactaa tttttgatct tctaaggatg tatttatcaa
     1861 tatccatgtc tttgatattc aaaagtggtt tgcctttttt tccaactttt ccaataactt
     1921 ctggttcaag atg
//
LOCUS       DATAFW010000072         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1266804, whole genome shotgun sequence.
ACCESSION   DATAFW010000072 DATAFW010000000
VERSION     DATAFW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266804"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..323)
                     /locus_tag="VHJ57_01720"
     CDS             complement(171..323)
                     /locus_tag="VHJ57_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487284.1"
                     /translation="MYRDKNIGHCRVLKGGRIRISKEVREKLSVKDGDFVDFLLKTLV
                     IFLPYS"
     gene            complement(393..>1685)
                     /locus_tag="VHJ57_01725"
     CDS             complement(393..>1685)
                     /locus_tag="VHJ57_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487285.1"
                     /translation="FISRNISLNNLHSRFPWTKKVLKTYNIVSINTNPDFILSEASVI
                     LNTIVNTLSNRIQKIEDPFIPLKEKPRPFIILEIWDNITKELDNNDIIRTQKILSSLI
                     DKNGGFIIFLTEDMNNTDIDNLIDGIITLDQSFYKSYRLREMQIYKLKGTGINRSKIA
                     FTLANGMFSTFSSLSHKINFDKKNNFIFLPHKEKIFSTGNQRLDNKLQGGFKKGSIIS
                     LEIEDEVDRFAFVPIFVPLVLNFVSQNYGALVISAWDQHTLAVTRYITPFVNKKFLNK
                     YFKIIGRTNEIKKAKKHNEQIPPYIISGIESDFINTFNMWEREYEELRTRTDGCLVTV
                     DFSFVELQYQNELIDIQKAIINFSSYIRSSNDLLVMVSRPNYKSLEVMKSVSDIHLKL
                     LEFNGAVMLAAIKPQFFLYNIQTDQNAGYPRSHFIESS"
BASE COUNT          568 a          245 c          219 g          653 t
ORIGIN      
        1 tcgttcttcc attgttttat catgttatag gttaatagtt acgtaaatac atagattgac
       61 aattatagta aactaccatt ggtcattttg gaataataaa taaagaattc tatatttaga
      121 aataaatcaa aaacaagaaa agcagtagat gatttgactt gtaactacaa ttaggaatat
      181 ggtagaaaga taactaatgt tttaagtaaa aaatctacaa aatccccatc ttttacgctc
      241 aatttttctc taacttcctt tgatattcta attcttccac ctttcaatac tctgcaatgt
      301 ccaatatttt tatccctata catgtaaata attatctttt actactaaaa accgttgagt
      361 taacagagcc tttcaaagaa tatttatatt tatcaagaac tttctatgaa atgtgacctg
      421 ggatatcctg cattttggtc tgtttgtata ttgtataaaa aaaattgagg tttaatagcc
      481 gccaacatta cagcaccatt aaattctaat agtttcaaat gaatatcact aacacttttc
      541 attacctcta acgatttata gtttggtctg gataccataa caagtaagtc attggaagaa
      601 cgtatataac ttgaaaagtt tataatggct ttttgaatat ctataagctc attttgatat
      661 tgtaattcta caaaactaaa gtcaaccgtg acaagacatc catcagttct agttcttaac
      721 tcttcgtatt ccctttccca catattgaaa gtattgataa aatcactttc tattcctgat
      781 atgatatatg gagggatttg ctcattatgt ttctttgcct tttttatttc attagttctt
      841 cctattattt tgaaatattt atttaagaat tttttattta caaatggagt aatataacgg
      901 gtgactgcta aggtatgttg atcccatgct gagattacca gtgctccata attttgtgaa
      961 acaaaattta atacaagtgg aacaaaaata ggtacaaacg caaatctatc tacttcatct
     1021 tctatttcta acgatattat acttcctttt ttaaaacctc cttgtaattt attgtctaat
     1081 ctttgatttc cggtagagaa tattttctct ttatggggta agaaaataaa attatttttt
     1141 ttatcgaaat taattttatg agaaagagac gaaaatgtac taaacattcc attagctaac
     1201 gtaaatgcta ttttcgatct atttatacca gttcctttga gtttataaat ttgcatttca
     1261 cgtagacgat atgatttata aaaagattga tcaagagtta tgattccatc gattaaatta
     1321 tcaatatcgg tattattcat atcttcagtc aaaaaaataa taaaacctcc atttttatct
     1381 attaaggaag ataatatttt ttgtgttctt ataatatcgt tattgtcaag ttcttttgtt
     1441 atgttatccc atatttctaa tattataaat ggtcttggtt tttcttttaa aggtataaac
     1501 ggatcttcaa ttttttgaat cctgttagat aatgtattca ctatagtatt taatatgact
     1561 gaagcttcag ataatataaa atctggattg gtgttgatag atactatgtt atatgttttc
     1621 aaaaccttct tcgtccaagg aaatctacta tgaaggttgt tcagcgaaat atttctagaa
     1681 atgaa
//
LOCUS       DATAFW010000073         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3910423, whole genome shotgun sequence.
ACCESSION   DATAFW010000073 DATAFW010000000
VERSION     DATAFW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910423"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="sufC"
                     /locus_tag="VHJ57_01730"
     CDS             complement(<1..654)
                     /gene="sufC"
                     /locus_tag="VHJ57_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX2487286.1"
                     /translation="MVFLQVKDLNVSIDGKKILNGLNLEIDKNEVHAIMGVNGSGKST
                     FANVLLGHPKYSVTSGEIIVKGKSILELSTDERAKQGIFLGFQYPIEISGVGYSHFLR
                     NAYNTLNKSLTEDQKDREVFLTVKEFHEYVKKNLNYVGLDQSFLGRYLNEGFSGGEKK
                     RSEVMQMLVLRPNIAILDEPDSGLDIDAVKAVAEAINKLINTGAGVVVITHYARILRY
                     "
     gene            complement(791..1294)
                     /locus_tag="VHJ57_01735"
     CDS             complement(791..1294)
                     /locus_tag="VHJ57_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX2487287.1"
                     /translation="MGLNEKIIFLGILGIGAIVGVISFTLFSDLPPTPGNTKSIYRQD
                     LPPPPPTAQASSSTESKVDPATVKADATLTILEGAATQGNPDYDPKELSVKKGNVIKV
                     ENADSLPHTVTNGEGPNDPNSAKIFDTSLIMGGEAGILQTEEIDAGEYTYYCTVHPYM
                     TGSLKVE"
     gene            1868..>2073
                     /locus_tag="VHJ57_01740"
     CDS             1868..>2073
                     /locus_tag="VHJ57_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487288.1"
                     /translation="MDKNLELLLDLSINILEKTDEKIILKFNNIPRQYLNAIRRIAIS
                     EVPTLAIDDVVILENSSVMHDEAIA"
BASE COUNT          722 a          311 c          281 g          759 t
ORIGIN      
        1 ataccgtaaa attctagcat agtgtgtaat tacaacaact ccagctcctg tattgatcaa
       61 tttatttatt gcctctgcaa ctgcttttac tgcatcaata tctaaacctg agtctggttc
      121 atctaatata gctatatttg gccttaatac caacatttgc ataacttcag atcttttctt
      181 ttctcctcca gagaaacctt catttaaata tcttccgaga aatgattgat ctaagcccac
      241 ataatttaga ttttttttaa catattcatg aaactcttta acggtaagga aaacttctct
      301 atctttttga tcttctgtta aagacttgtt taaagtatta taagcatttc ttaaaaaatg
      361 actgtaaccc acacctgaaa tctcaatggg gtactgaaat cctaaaaata ttccttgttt
      421 agctctttcg tcagtagata attctaagat gctttttcct ttaacaataa tttcaccaga
      481 agtaacagaa tattttggat gtcctaagag tacatttgca aaggtgcttt tacctgaccc
      541 atttacaccc ataatagcat gtacttcatt tttatcaatt tctagattta aaccatttag
      601 tatctttttt ccgtcgatac ttacattcag atctttaacc tgtaaaaaaa ccatagtcta
      661 ataatcttat taattacata taaagcattc attatctgaa aatttggtag attatctagt
      721 aaaacatgga taaccttgaa aatttcaata aatgtcttat ataaaaataa agtggttggt
      781 tatttatcag ttactcaacc tttaagctac ctgtcatata gggatgaact gtacaataat
      841 atgtgtattc acctgcatca atttcttcag tttgtaaaat tcctgcttct cctcccataa
      901 ttaaactcgt atcgaatatt tttgctgaat ttggatcatt cggtccttca ccatttgtaa
      961 ctgtatgagg aagagaatca gcattttcga ctttaattac attgcctttt ttaactgaaa
     1021 gttcttttgg atcatagtcc ggattacctt gtgttgcagc accttcaaga attgtcaatg
     1081 tcgcgtcagc tttaacagtt gcaggatcta cttttgattc tgtacttgaa ctagcttgtg
     1141 ctgtcggtgg aggaggagga agatcttgtc tatagattga ttttgtattc ccgggtgtag
     1201 gtggaagatc agaaaataga gtaaatgaaa ttactccaac aattgcacct attcccaata
     1261 ttcctaaaaa aataatcttt tcattgagtc ccatttaact gatcatggat tcgaataagg
     1321 tcttaattaa ttttttcaaa taaatactca agtttgagca aattttaatt atttttatta
     1381 cttaatgtaa atcaaagttg ttatatattt aaattgaaaa taaagataat aaaatattat
     1441 aaaattagaa tatagtgatt aagaagttct gcatttataa taatagattt aaaaacgata
     1501 caattattat aatatttata attctaaaaa taatgaaaga aatgtctgct tatacaaaat
     1561 gttctatctg taacaatcaa atttctttat ttagatataa agcaatgaaa caatggaata
     1621 ttcatggata tttatgtaat gattgttatt ctaaaaagtt aacagaacac tatatttata
     1681 gaaaagaaaa tagtaattat aataaaaaaa ctgatttata atttactaat taatctttta
     1741 tgattagaat attgaatcat attagaattt acatagatca attcaattca atttattctt
     1801 acgttagaat ctattgatat cataacctcg tttatattgt agtagtcggt agatttttaa
     1861 gagtgcattg gacaaaaatc ttgaattact attggactta tctataaata ttttggaaaa
     1921 gacagatgaa aaaataatac taaaatttaa taatattcca agacagtatt tgaatgctat
     1981 cagaaggatc gcgatcagcg aagttccaac attagcaatt gacgatgtag ttattctaga
     2041 aaattcatcg gttatgcatg atgaggcaat agc
//
LOCUS       DATAFW010000074         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5496153, whole genome shotgun sequence.
ACCESSION   DATAFW010000074 DATAFW010000000
VERSION     DATAFW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5496153"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VHJ57_01745"
     CDS             <1..303
                     /locus_tag="VHJ57_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX2487289.1"
                     /translation="NISQKAIICFNPAVAPSGITFASSNKLGYQNDLIITTLKGSHLR
                     QIDVESGEQNNILVGYGRLRDIIESSDGTVYVLTSNTDGRGIIQDNDDKILKILKP"
     gene            310..1644
                     /locus_tag="VHJ57_01750"
     CDS             310..1644
                     /locus_tag="VHJ57_01750"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEX2487290.1"
                     /translation="MDKINKKDNNKNIKKFYELDHQERLQYLKKEARLTEQDSEIFQD
                     FPKNFAFKKINNMIENAIGFVPIPLGIATNFVINGRKYLIPMAIEEPSVIAAASKAAK
                     IASIKGGFLAEADNSIMIGQIQLVSRDKRNNKDKFFYKKERDKIKKIILKNKEKLLEI
                     SNSKSRSVHAIDLQLREMSDESNNKMGHMIIIELIIDTRDAMGANVINTMCELVGSEL
                     EKITKCTVILKILSNYSTKRMARCKAIFSKELIGGSNGVNRILYAYAFAYSDIYRAVT
                     HNKGLMNGIDGVAMATGQDIRAIEAAAHAYASNDGKYRSLTKWYRSEEGDLVGEIELP
                     LAIGIVGGISSVHPYVKVALKILNIKKAKELASIMVAVGLAQNFAAIRALADEGIQKG
                     HMRLHARNIAITAGATDEQINTVASRLIKENNVTVNKAEEIIELINKNENLY"
     gene            1688..2668
                     /gene="hisG"
                     /locus_tag="VHJ57_01755"
     CDS             1688..2668
                     /gene="hisG"
                     /locus_tag="VHJ57_01755"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX2487291.1"
                     /translation="MAHIVKFVVPKGTIEEATLKILEEAWQKISGRARTYRIKLSDPE
                     IEVKLLRPQEIPTYVQEGLYDVGITGRDWIKETNADVKILLDLEYGRVKQVIAIPESF
                     QFNNLDEMIIDHVRREKPLRISAEYLTTTADYIKSKIAYKKYFGNSNPMIITPWLRVG
                     ENKNVQIFLSFGATEAKPPEDVDAICDITETGTTLAQNNLKIIDTIMESTSVLIANKN
                     SLKDNKKREKIADMIALLRGVVEARKKLHIFVNVRKENLDELLSTLPALKRPTVSNLS
                     EEGWYGVNTIIDKNEFIKIVPIMRKLAQGLVVTEPRQILPLEEINIDKEE"
     gene            2669..3994
                     /gene="hisD"
                     /locus_tag="VHJ57_01760"
     CDS             2669..3994
                     /gene="hisD"
                     /locus_tag="VHJ57_01760"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX2487292.1"
                     /translation="MINRITIKNAKLDVESVRYRISSNFSDDAIKKTQEIIKNVRNFG
                     DDAIKRYTSTYDKVELDSFQVNREEIEEAYNCISEDHIKTIKLIRNRLIKNETILLEH
                     LKKISSLYIRNKNIQRIIQPISSVGCYVPGGSARYPSTLIMCVTPAKLAGVKRIVAIT
                     PTKINGKIDPLTLVSADICGVNEIYKIGGAQGIAGLAFGTNSIKRVDKIVGPGGMFVT
                     IAKLLVSSIVSIDMLAGPTELVIYADDKSNPKYVALDLISQSEHSKDTLCGLVTNSYE
                     FANTVEDEINNILESNISRQEIVTNSLKENGFIAISENEDLAIEFINEIAPEHLQIMA
                     KNELKIVKKITSAGLTLIGENTPSAASDYCLGSNHVLPTMGFGKSRTSLSILDFVRIS
                     NIVKASKKELKEIEPYVKIITEEEGLVNHYKAIKERVIDIPKQKIEDEK"
     gene            3997..>4294
                     /locus_tag="VHJ57_01765"
     CDS             3997..>4294
                     /locus_tag="VHJ57_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487293.1"
                     /translation="MDKWFESKLSELEKLDYYKTPEKSKGLKLDANENLALKKDFIQK
                     IAIKAAERQDLREYPLQQVDDFYLQLSKYTKIDKKYLAIGNGSDQIIDLILSVFG"
BASE COUNT         1755 a          529 c          765 g         1245 t
ORIGIN      
        1 aatatttctc agaaagctat tatttgtttt aatcctgcag ttgcaccttc tggtataacg
       61 tttgcttcat caaataaact aggttatcaa aatgatctaa tcataacaac tctcaaaggt
      121 tctcatttaa gacaaataga cgtagaatca ggagaacaaa ataatatatt agtaggttat
      181 ggtcggctta gagatattat tgaatctagt gacggtactg tatatgtact aactagcaat
      241 accgatggaa gaggaattat ccaagataac gatgataaaa ttttaaaaat acttaaacca
      301 taaactcttg tggacaaaat taataagaag gataacaaca aaaatatcaa aaaattctat
      361 gaattagatc atcaagaacg tttgcaatat ttgaaaaaag aagctcgctt aactgagcaa
      421 gattcagaaa tcttccagga ctttccaaaa aattttgctt ttaagaaaat taacaacatg
      481 attgaaaatg caatcggatt tgtacccatt ccgttaggaa ttgctactaa ttttgttata
      541 aatggaagaa aatatttaat tccaatggct atagaagaac catctgttat cgctgctgca
      601 agtaaagctg ccaaaattgc aagtataaaa ggaggatttt tagctgaggc agataattcc
      661 atcatgattg gacagatcca attagtttca agggataaaa gaaataataa agataaattt
      721 ttttacaaaa aagaaagaga taaaataaaa aaaataatat taaaaaataa agaaaagtta
      781 ttggaaatat caaactcaaa aagtaggtcc gtacatgcta ttgatttaca attaagggaa
      841 atgtctgatg aaagcaacaa caaaatgggc cacatgataa taatcgagtt aattatagat
      901 acaagagatg caatgggagc aaatgtaatt aatacaatgt gtgaattagt tggttcagaa
      961 ttagaaaaaa taactaaatg tacagttatt ttgaaaattc tttcgaatta ttcaacaaaa
     1021 agaatggcta gatgtaaagc tattttttca aaagaattga ttggtggctc gaatggtgtg
     1081 aatagaattt tatacgccta tgcatttgca tattctgaca tatatagagc tgttacacat
     1141 aacaaaggtt tgatgaatgg aattgatgga gtagctatgg ctacaggaca agatattaga
     1201 gctatagaag cagctgcaca tgcatatgct tctaatgatg ggaaatatag atctttgact
     1261 aaatggtata gatcagaaga aggagattta gttggagaaa tcgagcttcc attggctata
     1321 ggaatagtag gagggataag ctcagtacat ccatatgtta aagtagcttt gaaaatatta
     1381 aatataaaga aagccaaaga gcttgcttca ataatggtag cggtaggact agctcaaaat
     1441 tttgctgcaa ttcgtgcttt agcggatgaa ggaattcaga aaggacatat gagattacat
     1501 gcgcgaaata tagccataac tgcgggagca accgatgaac aaatcaatac cgttgcaagc
     1561 agacttatca aagaaaataa tgttactgta aataaggcag aagaaattat agaattaatt
     1621 aataaaaatg aaaatttata ctaagtagat atatatccga tgaaaagaaa aattttatgt
     1681 tatagttatg gctcacatag taaaatttgt tgtacctaag ggaaccattg aagaggcaac
     1741 tttaaagatt cttgaagaag catggcaaaa aataagtggt agagcacgaa cttatagaat
     1801 taagttaagt gatccagaaa tagaagttaa acttctgagg ccacaggaaa ttcctacgta
     1861 tgtacaagaa ggactatacg atgttggaat tacagggaga gattggatta aagaaacaaa
     1921 tgcagatgta aaaatattgc ttgatttaga atatggtaga gtaaaacaag taatagctat
     1981 accagaaagt tttcaattta ataatttgga tgaaatgatt atagatcatg taagaagaga
     2041 aaaaccactg agaatatctg cagaatatct tacgactaca gctgattata taaaatctaa
     2101 aattgcctac aagaaatatt ttggaaatag taatcctatg attattaccc cttggcttag
     2161 agttggcgaa aataagaatg ttcaaatatt tctctctttt ggggctacag aagctaagcc
     2221 accagaagat gtagatgcca tatgtgatat tactgaaact ggaacaactt tagctcaaaa
     2281 caatttaaaa attatagata caattatgga atctacttct gttctaattg caaataaaaa
     2341 ttctcttaaa gacaataaaa agagagagaa gatagcagat atgattgcat tattaagagg
     2401 agtagtagaa gcacgtaaaa aactgcatat ttttgttaat gttagaaaag aaaatttaga
     2461 tgaattactt tctactttgc cagcattaaa gagacctaca gttagcaatt taagtgaaga
     2521 aggatggtat ggagtgaata ccataatcga taagaacgaa tttataaaaa ttgttccgat
     2581 aatgagaaaa ttagcacagg gtttagttgt aacagaacca agacaaatat taccattgga
     2641 agagatcaac atagataaag aagaataatt gattaataga ataacaatta aaaatgctaa
     2701 attagatgta gagagtgtac gttatagaat atcgagtaat ttttctgatg atgcaattaa
     2761 aaaaacacaa gaaattataa aaaatgttcg aaattttggt gatgatgcta taaaaagata
     2821 cacttctaca tatgataaag tagaactaga ttcattccaa gtcaataggg aagaaataga
     2881 agaagcgtac aattgtattt ctgaagacca tattaaaact attaaactta ttagaaatag
     2941 attgataaaa aatgaaacta ttcttctaga acatcttaaa aaaatatcat cattatatat
     3001 ccgaaataaa aatatacaac gcataatcca acctatttct agcgttggtt gttatgttcc
     3061 tggtggatct gccagatatc caagtacact aataatgtgt gtaacaccag caaaattagc
     3121 aggagttaaa agaatagtag caataacacc aactaagata aatggaaaaa tagatcccct
     3181 tacgttagtt tctgctgaca tttgcggagt taatgaaata tacaaaattg gaggagcaca
     3241 aggtattgca ggattagcgt ttggaactaa tagtattaag agagtggata agattgtagg
     3301 tccaggagga atgtttgtaa caattgcaaa attgttggtt tcaagtatag tatcaattga
     3361 tatgttagca ggtcctacgg aattagtaat ttatgcagat gataaatcta atcctaaata
     3421 tgttgcttta gacttgattt cccaatcaga acacagcaaa gatacgttat gtggattagt
     3481 aactaattca tatgaatttg caaatacagt tgaagatgaa attaataata ttttagaaag
     3541 taatatttca cgccaggaaa tagttacaaa tagtttaaaa gagaatggat ttattgctat
     3601 atcagaaaat gaagatttag caatagaatt tattaatgaa atagctcctg aacatttaca
     3661 gattatggct aagaatgaat taaaaatagt taaaaaaatt acatctgcag gacttacttt
     3721 aattggagaa aatactccat ctgcagctag tgactattgt ttaggctcaa atcacgttct
     3781 tcctactatg ggatttggca aatcaagaac atctttatcg atattagatt ttgttcgtat
     3841 ttcaaatata gttaaggctt caaaaaaaga acttaaggaa attgagcctt atgtcaagat
     3901 aataactgaa gaagaaggat tggttaatca ttataaagcc attaaagaaa gagtaataga
     3961 cattccaaaa caaaaaatag aagatgagaa ataaagttgg ataaatggtt tgaatcaaaa
     4021 ttaagtgaat tagaaaaact agattattac aaaacacctg aaaaaagtaa aggattaaaa
     4081 ctggatgcaa atgaaaatct tgctttaaaa aaggacttta tccaaaaaat cgctatcaaa
     4141 gctgcagaaa gacaggattt aagagaatat ccattacaac aagttgatga cttttattta
     4201 cagctttcaa aatatactaa aatagataaa aaatatttag ctattggaaa tggctctgat
     4261 caaataatag atttaatcct gtcagtcttt ggaa
//
LOCUS       DATAFW010000075         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_738819, whole genome shotgun sequence.
ACCESSION   DATAFW010000075 DATAFW010000000
VERSION     DATAFW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738819"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1787
                     /gene="carB"
                     /locus_tag="VHJ57_01770"
     CDS             <1..1787
                     /gene="carB"
                     /locus_tag="VHJ57_01770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX2487294.1"
                     /translation="KQLGYYFHLDELQVRHIRKTFRIEPVIKQIDTLAAEWPAQTNYL
                     YLTYGGDYDDVRIKNKGKNAIVLGAGPYRIGSSVEFDWGTVNMVIGLKNNGISEVSII
                     NCNPETVSTDFDVSDRLYFEEITLERVLDIYDKEKSDGIITCVGGQTANNLTQSLAKN
                     GAKIIGTSSYDVDRAEDREKFSKLLDSLKITQPPWRKFTNLEEAKTFSESVLYPVLVR
                     PSYVLSGAAMKVVWDDKQLEKFIDNATIVSPDHPIVISKFFQDASEVEVDGIGSKDKI
                     IIGSIIEHIDNAGIHSGDAMMCLPPWRLTRNIMDTITDYSNKIVRALNVSGPFNIQFL
                     VKNEEVLVIEANIRASRSMPFVSKYIGVNLIDLAARAMLNKPLPNIHEQLWLKTSGFA
                     IKVPQFSFMQLEGSDIVLGVEMHSTGEVACFGTSFYDALSKSYIAAGYSLPKKGTSLI
                     TIGGQKNKEKLLPVISLMTSMDYKIMATEHTADFLEMNGIKDIQRVYKINEPERKPNI
                     IDLLYQHKIDFIINIPSTSTMEKYLNMLNDEYYIRRKAVEMGIPVMTTLEASSAFIKT
                     LQWLQTNSPTIEPLKKYLKINDSITNLE"
     gene            complement(1779..>2357)
                     /locus_tag="VHJ57_01775"
     CDS             complement(1779..>2357)
                     /locus_tag="VHJ57_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX2487295.1"
                     /translation="HSFNAIIILGDLKSSITHITKNEWETIPIFLKELSLDMDIFLIP
                     GNHDANINLLVPDSVIVCNPSGLIIDDILLIHGHTIPQKTQFNINKIIMGHIHPIFIN
                     SNSVLHGKRLWIYLKVKKESIFGLKTKGIIEIIIIPTFNKYFYSLGYHKESRSISPLI
                     RQIIKNDAILSGSLFTLEGSLIGETQEIKNLF"
BASE COUNT          856 a          336 c          407 g          758 t
ORIGIN      
        1 ataaacaatt aggttattat ttccatttag atgaactgca agtcaggcat attcggaaaa
       61 catttagaat tgaaccagta ataaaacaaa ttgatactct ggccgcagaa tggccagccc
      121 agacaaatta tctatatctt acatatggtg gagattatga tgatgtcaga atcaaaaaca
      181 aaggtaaaaa tgccatagtt ttgggtgcag gtccatatag aattggaagt agtgttgaat
      241 ttgattgggg aacagtaaat atggttatcg gtttaaaaaa caatggtatc agtgaggttt
      301 caataattaa ttgtaatccc gagaccgttt caacagactt tgatgtttca gatcgattat
      361 acttcgaaga gattacgttg gaaagggttc ttgatatcta tgacaaagag aaatctgatg
      421 gtataataac atgcgtagga ggtcaaactg ccaacaattt gacccagagt ttggcaaaga
      481 acggtgcaaa aataataggc acttcaagtt atgatgttga tagagcagaa gatagagaaa
      541 agttttccaa acttttagat tccttgaaga ttacacaacc tccttggaga aaatttacga
      601 atcttgagga ggcaaagact ttttctgaat cagtacttta tccagtattg gtaagacctt
      661 cctacgttct tagtggtgca gccatgaaag tagtctggga tgataagcag ttagaaaaat
      721 ttatagataa tgcaactatt gtaagtccag atcatccaat agtaataagt aaatttttcc
      781 aagatgcttc tgaagtagag gtagatggta taggttcaaa agataagata attattggat
      841 ctataataga acatatagat aatgctggta ttcactcagg agatgcaatg atgtgtttac
      901 caccatggcg attaacaaga aatattatgg atacaataac agattattct aataagatcg
      961 taagagcatt gaatgtttca ggacctttta atattcagtt tctagttaag aatgaggaag
     1021 ttttggtgat agaagccaat ataagagcat ctagatctat gccttttgtt tcaaaatata
     1081 taggcgtaaa tcttattgac ttagctgcaa gagcaatgtt gaataaacct ttacctaata
     1141 ttcacgaaca actctggctg aaaacaagcg gattcgctat aaaagttcct caattttcct
     1201 tcatgcagtt agaaggatct gacatagttc ttggtgttga aatgcattct actggcgaag
     1261 ttgcatgttt tggaacaagt ttttatgatg cactaagtaa atcttatata gctgcaggtt
     1321 attctttacc aaaaaaagga acttcattaa ttacaatagg aggacagaaa aataaggaaa
     1381 aattactacc agttatatct cttatgacat caatggatta taaaatcatg gctaccgaac
     1441 acactgctga tttcttagaa atgaacggaa ttaaagatat tcaaagagtt tataaaataa
     1501 acgaacccga aagaaagcca aatattattg atcttttgta tcaacataaa atagatttta
     1561 ttatcaatat tccgtctact tccacgatgg aaaaatacct gaatatgtta aatgatgaat
     1621 actatataag aagaaaagct gtagaaatgg gtattcctgt aatgaccaca ttggaggcct
     1681 cttcggcatt tataaaaact ctccaatggc tacagacaaa ttcacctact attgaaccgc
     1741 ttaaaaaata tttaaaaata aatgattcaa taaccaactt agaataaatt ttttatttct
     1801 tgagtttctc ctataaggga tccctctaac gtgaacaagg atcctgaaag gatcgcatca
     1861 tttttgatta tttgtctaat tagtggtgat atagatctag actctttatg ataccctaaa
     1921 gaataaaaat atttattaaa agtaggaata attattattt caattatacc tttcgttttt
     1981 aatccaaaaa tagattcttt ttttaccttt agatatatcc ataatctttt accatgaaga
     2041 acgctattgg aattaataaa aataggatga atatgaccca taataatttt gtttatatta
     2101 aattgagttt tttgagggat tgtgtgtcca tgaattaata atatatcatc aataattaat
     2161 ccactaggat tacatactat taccgaatct ggtaccaata aatttatatt cgcatcatgg
     2221 tttcctggaa ttaaaaatat atccatatct aaagataatt ctttaagaaa aataggtatt
     2281 gtttcccatt cattttttgt aatatgggtg atggagcttt ttaaatctcc caaaataata
     2341 atggcattaa aggaatg
//
LOCUS       DATAFW010000076         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6202014, whole genome shotgun sequence.
ACCESSION   DATAFW010000076 DATAFW010000000
VERSION     DATAFW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6202014"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VHJ57_01780"
     CDS             complement(<1..714)
                     /locus_tag="VHJ57_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX2487296.1"
                     /translation="MNSIISTRIMILAPQELENSASKYAAEAIRLDSQGSRLKAIQFY
                     QRAIEALNKLIQIYPEYKLNKIYLERSNAYLNRIKSLQMSNVLEDEEGKNGDENIIGN
                     DADNSDRKIGSEMRKPKEPLGEEFDNLIMKEKPNISWNDVIGLDDAKRAIRESIVYPA
                     KRSDLFPLGWPRGILLYGPPGCGKTLLAAAAAAEIDGYFVNVDAAAMMSKWLGEAEKN
                     VSKLFRMANKLYESENIPIL"
     gene            complement(875..2155)
                     /locus_tag="VHJ57_01785"
     CDS             complement(875..2155)
                     /locus_tag="VHJ57_01785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF037510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2487297.1"
                     /translation="MSEILLLKFPFELKKDQRDAVDEWINKKGKGSIIYSTGTGKTEI
                     AFECARKMASLIRQQNPTKVLKILFLVPRIVLIEQNIKRLIKYGIKKEKIGVYYGEKK
                     DIKEITFSTYQSIIKNFDLLRQSDMIILDEMHMVSKTAKRLSKIFDILHDNYDKLILG
                     LTATIEENDPRYSEIMNLIPPIKKYMIKEAVNDGRLSEPQVILKSVKLNLEERRIYEE
                     TTSTIKDISFKLKASNPLIVSRLLKTGGQRARLAKVWFASVHKRKKLLNETNSKLNET
                     VNIIKNHPNEKIMIFSETIESINKISEILKKNKIPSEVIHNKVKTKLRQEILDSWGKN
                     YFVLLSVHTLEIGFDIPSVSIAIIVSNTKNMHQLIQRIGRVIRKTDEKSQSLIYVVYV
                     DDTKDKNILNLVRASLQTNSIKKPIKQDKLSSYF"
     gene            2290..2796
                     /locus_tag="VHJ57_01790"
     CDS             2290..2796
                     /locus_tag="VHJ57_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HEX2487298.1"
                     /translation="MKVFVLELKQDDPNKCTAAKLVKFNLIQSTKFIPKDTIVLNPLS
                     KIALSKTDNSIAKSICAIDCSWNYFNETIKKFKILKKRINRRLPLLLAGNPVNYAKLG
                     KLTTAEAIAGSLFILGFRSKAEELLNKFKWGHTFLDLNIELLDLYKNQEDPDAILKIE
                     EDYFPNLY"
BASE COUNT          925 a          483 c          377 g         1164 t
ORIGIN      
        1 taatatagga atattttcac tttcgtataa cttattagcc attctaaaaa gttttgaaac
       61 atttttctca gcttcaccaa gccatttact catcattgca gctgcatcaa catttacaaa
      121 ataaccatca atttccgcag ctgcggctgc agcaagtaag gttttcccac atcctggagg
      181 tccatatagt aatattcccc taggccatcc taacgggaat aaatcagatc tcttagcagg
      241 gtaaactata gactccctta ttgctctttt agcatcgtca agaccaatta cgtcattcca
      301 agaaatatta ggtttttctt tcataattaa attatcaaac tcttcgccaa gtggttcctt
      361 tggcttcctc atctcacttc ctatttttct atcagaattg tcagcatcat tcccaattat
      421 attttcatca ccattctttc cttcttcatc ttccaaaaca tttgacattt gtaacgattt
      481 gatcctgttt aaataagcgt tagatctttc taagtatatt ttatttaatt tgtattcggg
      541 ataaatttgt attaatttat tcaaagcctc aatagctcgt tgataaaatt gaatagcctt
      601 taatcttgaa ccttgagagt ccagccttat cgcttcagca gcatatttac ttgcactatt
      661 ttctaattcc tgtggagcta aaatcataat tctagtcgat attattgagt tcataattga
      721 tttttagtac tatgatgtaa atcttatttg ttctaaaaac taaaaatagt ataccaataa
      781 attttaacat tagcaatatt ttggattcgt tttcttttac actgattatt gttaaatcaa
      841 gaaatacttt gataaaatcc attcctataa ttgactaaaa gtaggatgag agtttatctt
      901 gtttaatggg tttttttata ctattggttt gtaatgatgc tcttaccaag ttaagtatgt
      961 tcttatcttt tgtatcatct acatacacca catatattag agactgactt ttttcatctg
     1021 tttttcttat tactcgtcct attctctgta taagttgatg catatttttc gtatttgata
     1081 ctattattgc tatacttaca gacggtatat caaatccaat ctccaaagta tgtactgata
     1141 gcaatacaaa ataatttttc ccccatgaat ctaaaatttc ttgtcttagt tttgtcttta
     1201 ctttattatg tataacttct gagggaattt tatttttctt taaaatttca cttattttat
     1261 ttatggattc tattgtttca ctaaatatca tgattttttc atttggatga tttttaatta
     1321 tatttacagt ctcatttaat tttgagttag tttcattaag taattttttt cttttatgaa
     1381 cacttgcaaa ccataccttc gctaatcggg ctctttgtcc acctgttttt aataaccttg
     1441 aaactatcaa tggattagat gctttaagtt tgaatgaaat atctttaatt gtgctggttg
     1501 tttcttcata tattcttcgc tcttctaaat ttaatttcac cgattttagt ataacctgag
     1561 gttcacttaa tcttccatca ttcacagctt cttttatcat atattttttt attggaggta
     1621 ttaaattcat tatctctgaa tatcttggat cattttcctc aattgttgct gttaaaccta
     1681 ggattaattt atcataattg tcatggagta tatcaaaaat tttgcttaat cttttggcag
     1741 ttttacttac catgtgcatt tcatcaagaa taatcatgtc tgattgtctt agcagatcaa
     1801 agttttttat aatgctctga taggtactaa aggtaatttc tttaatatct ttcttttctc
     1861 catagtaaac tccgatcttt tcctttttta taccatattt tattagtctc tttatatttt
     1921 gttctattag tacaattcga ggaaccaaaa aaagaatctt aaggaccttt gttggattct
     1981 gttgtctaat tagagaagcc attttccttg cacattcaaa agctatttcc gtttttcctg
     2041 taccagtact ataaataata gaacctttgc cttttttatt tatccattca tccactgcat
     2101 ctctctgatc tttttttaat tcaaaaggaa attttagaag caatatttcg gacaagaatg
     2161 ataaagtatc aaagaagtta ttaataatta cttagtttat tttgattttt ttttatacgc
     2221 gagttcagaa tgtttgtatt aatactttta acttcaaata aataattgga tattttaata
     2281 actatagtaa tgaaagtttt cgttttagaa ctaaaacaag atgatccaaa taaatgtact
     2341 gcagccaaat tagttaagtt taatttaatt caatcaacta aatttatacc gaaagacact
     2401 atagttttaa acccattatc taaaattgca ttatctaaaa cggataatag tatagctaaa
     2461 tccatctgtg ctatagattg ttcttggaat tattttaatg agactataaa aaaattcaaa
     2521 attcttaaaa aaagaattaa tcgacgtctt ccactacttc ttgcaggaaa tcctgtaaat
     2581 tatgcaaagt taggaaaatt aactactgct gaagctatag cgggttcttt atttatacta
     2641 ggttttcggt caaaggcaga agaattatta aataaattca aatggggtca tactttttta
     2701 gacttaaata ttgaactatt agacttatac aaaaatcaag aagaccctga tgcaattctt
     2761 aaaatcgaag aagattattt tccaaatcta tattagttaa tattgtacaa taatattgtg
     2821 agctagcgta cgagtgactc gaaagagagg aaactcctcc ctcatagcag gcactcaaat
     2881 tagcgataat tagctggaga cagctggcaa cgtaacagaa aagtatctag aagacttata
     2941 ataactcta
//
LOCUS       DATAFW010000077         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_211926, whole genome shotgun sequence.
ACCESSION   DATAFW010000077 DATAFW010000000
VERSION     DATAFW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211926"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rplF"
                     /locus_tag="VHJ57_01795"
     CDS             complement(<1..305)
                     /gene="rplF"
                     /locus_tag="VHJ57_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2487299.1"
                     /translation="MSTDKDGNQSGEVTIPENIKVTKEENTVIVEGKNGKIAKDFTKI
                     PITLVFENNKIIIHPPGQRRRDLALINTVKSIISNMFKGVETGFTYKLKIIFSHFPIS
                     "
     gene            complement(295..687)
                     /locus_tag="VHJ57_01800"
     CDS             complement(295..687)
                     /locus_tag="VHJ57_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX2487300.1"
                     /translation="MPSLNVVSNLFNTLYNNEMRRKKECLVIPTSKFASEILRVMQKY
                     RYIGEFELIDDNRMGKFRIQLLAKINKCAIITPRYGVKKNEFLNWERQFLPAYNLGIL
                     LVSTSKGIMSHTDAQSQGLGGVLIGYVY"
     gene            complement(782..961)
                     /locus_tag="VHJ57_01805"
     CDS             complement(782..961)
                     /locus_tag="VHJ57_01805"
                     /inference="COORDINATES: protein motif:HMM:NF004424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX2487301.1"
                     /translation="MPKPREYSETGRKKVPHGKGTRWCKRCGAFNGLIQKYDIMLCRQ
                     CFRDVALNIGFKKYE"
     gene            complement(961..1488)
                     /locus_tag="VHJ57_01810"
     CDS             complement(961..1488)
                     /locus_tag="VHJ57_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX2487302.1"
                     /translation="MSENKINSSEVMKKIDIEKIVINIGVGKSGDPFERAKMALTELT
                     GQKPSPRGAKKSVRDFGIHKGEPIGAMVTLRSQNALDFLKRIFLAKSNSFKRSSIDNN
                     GNLSIGIKEHIDIPGIKYNPDIGIFGMDVCISLKRPGYRISKKRHPDKIGKKHRITKE
                     ESINFFQQNFGVEVI"
     gene            complement(1488..2216)
                     /locus_tag="VHJ57_01815"
     CDS             complement(1488..2216)
                     /locus_tag="VHJ57_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HEX2487303.1"
                     /translation="MANKGGRTTAKRQLAPVFWDIKRKQGRFILRTHPGTHSKKYAYS
                     LGVILRDILKVTTTMHEAKKIVDAGKVKVDNVIRYNVNFPIGLMDTIELVSSQKVYRL
                     VPKNSLLLYPIEINDNEKLLKLVKIKTKNIIKGNKIQYGFHDGKTVISEGIYSVGDTC
                     LVKVPELEIIDTLKLQSDSLAMLTRGDNAGSMGKIEEIKEGTFSLPKRAIMRVDSRLL
                     EIPIDMIMVVSEPNDNNPKIKVNE"
     gene            complement(2254..2766)
                     /gene="rplX"
                     /locus_tag="VHJ57_01820"
     CDS             complement(2254..2766)
                     /gene="rplX"
                     /locus_tag="VHJ57_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX2487304.1"
                     /translation="MINKNITLNIKKHNLDKHIRSPLSENLRNEYNKKNARVVKGDTV
                     KVLRGEYKNVEGKVEKVKTGRSSLFIEGIQREASKGGKVKVQIHSSNVIITSLNLHDK
                     NRSNVIRNVKSSKVDEKKGVNINPNLRKTRTRRKLNVKKILKPQQNRITNSKSSQDNK
                     GQDDYDGVRK"
     gene            complement(2782..>3118)
                     /locus_tag="VHJ57_01825"
     CDS             complement(2782..>3118)
                     /locus_tag="VHJ57_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HEX2487305.1"
                     /translation="DNTGAKILRIVQVTKYKGRHSRMPSGAVGDLVSVTVKKGPAQLR
                     KQIFGAVIIRQKYPLRRLSGLRLTFEDNAAVLVTPEGEIKGTDIKGPVAAEAAEKWPR
                     IANLASSIV"
BASE COUNT          877 a          581 c          408 g         1252 t
ORIGIN      
        1 gatattggaa agtgagaaaa gattattttg agtttataag taaatccagt ttctactcct
       61 ttaaacatat tagaaataat gctttttaca gtgtttatta aagccaaatc tcttcttctt
      121 tgaccaggtg gatgaataat gatcttatta ttttcaaaaa ctaatgttat aggtattttg
      181 gtaaaatctt tagcaatttt gccattttta ccctcaacaa ttactgtatt ttcctctttg
      241 gttactttga tattttctgg aattgttact tctccagact gattgccatc tttgtcagta
      301 gacatagcct ataagtacgc ctcctaatcc ttgactttga gcatcagtat gagacattat
      361 gcctttactt gtggaaacta atagtattcc taggttgtag gccggtaaaa actgtctttc
      421 ccaattgaga aattcattct tttttacacc atacctcgga gtgataattg cgcacttgtt
      481 tattttagct aataattgaa ttctaaattt tcccattcta ttgtcatcga tcaattcaaa
      541 ttctcctata taccggtatt tttgcataac tctgagaatt tcactagcaa atttagatgt
      601 aggtataact agacattctt tttttcttct catttcgtta ttgtataaag tattaaacaa
      661 atttgaaaca acatttaatg atggcattta aagaatattc tccaacactg tattaatttt
      721 ttccaacctt agcatggttt ttttatctaa tttctttatt ttttcgatat caatatatat
      781 tttattcata tttcttgaat cctatattaa gtgctacatc cctaaaacat tgtctacaga
      841 gcattatatc atatttttga attaatccat taaatgctcc acatctttta caccatctcg
      901 ttcctttacc atgtggaacc ttttttctac ccgtttctga gtactcacga ggcttgggca
      961 ttatatcact tcaactccaa aattttgctg aaagaaattt attgattctt cttttgttat
     1021 tctatgcttc ttaccgattt tatcaggatg tcttttcttg gagattctat aaccaggtct
     1081 tttcaaagaa atacagacat ccattccgaa gattccaata tctggattgt attttattcc
     1141 tggtatgtct atatgttctt taattccaat ggaaaggttg ccattattat cgatagacga
     1201 tcgtttgaaa gaattcgatt ttgctagaaa aattctcttc aagaagtcca gtgcgttttg
     1261 gcttcttaaa gtaaccattg caccaattgg ctcacctttg tgaataccaa aatcccttac
     1321 cgactttttg gctcctctag gactaggttt ttgtccggtc aattctgtca aggccatctt
     1381 agctctttcg aaaggatctc ctgattttcc cacccctata tttattacaa ttttttcaat
     1441 atctatcttt ttcattactt cacttgaatt gattttattt tcagacatca ttcattcact
     1501 tttatctttg gattattatc attaggttca ctaacaacca taatcatatc aataggaatt
     1561 tccaataatc tagaatctac tctcattatt gcacgtttag gaagagaaaa tgtcccttct
     1621 tttatctctt ctattttgcc catgctacca gcattatcac ctcgtgtcaa cattgcaaga
     1681 gaatctgatt gtaattttag cgtatcaata atttctaatt ctggtacttt aacaagacat
     1741 gtatcaccta ctgaatatat accttcagaa ataacagttt taccgtcatg aaaaccatac
     1801 tgaattttat ttcctttaat aatattttta gttttaatct ttacaagttt aagtaacttt
     1861 tcattatcat taatttctat tggatataac aatagtgaat tctttggtac taatcgataa
     1921 accttttgag atgaaactaa ttcaatagta tccattaaac ctatcggaaa gttaacatta
     1981 tatctaatga cattatctac tttgacttta cctgcatcaa caattttttt tgcttcatgc
     2041 atagttgtgg taacctttaa tatatctcgt aatataacgc ctagagaata ggcatatttt
     2101 tttgaatgtg tacctggatg ggttcttaga ataaaacgtc cctgttttct ttttatatcc
     2161 caaaacactg gagctaattg tcgttttgca gtggttcgtc ctcctttatt tgccattctt
     2221 tattttctcc ctatataata cattgatatc attttatttt ctaacaccat cataatcatc
     2281 ttgcccctta ttatcttgac tagattttga atttgtgatt cgattttgtt gtggtttaag
     2341 aatcttttta acattcaact ttcttcttgt tcgagttttt cttaaattgg gattaatatt
     2401 tactcctttc ttttcatcaa cctttgatga tttaacgttt ctaataacat tagatcgatt
     2461 cttgtcatgc aagttaaggg atgtaataat tacgttagaa gaatgtattt gaaccttcac
     2521 tttacctcct tttgaagctt ctctttggat tccttcaata aagagagatg atcttccagt
     2581 cttcactttc tctactttac cttcaacgtt tttgtattct cctcttaata cctttacagt
     2641 atctccttta accactcgtg cgtttttttt attatattca ttacgtaaat tttcagaaag
     2701 aggtgaacga atgtgtttgt caagattgtg tttcttaata tttaaagtaa tgtttttatt
     2761 tatcatagaa atcactataa tttaaacaat tgatgaggcc agatttgcta ttctaggcca
     2821 tttttcagct gcttcggcag caacaggtcc ttttatatcc gttcctttaa tttcaccctc
     2881 tggagttact aaaacagctg cattgtcttc aaatgtaagt cttaaaccac tcagtctcct
     2941 aaggggatat ttttgtctta tgataacagc tccaaaaatt tgtttcctca actgtgctgg
     3001 gcctttcttt acagtaacac tcacaagatc acctacagcc cctgaaggca ttcgtgaatg
     3061 acgaccttta tattttgtta cctgaacaat cctaagaatt ttggctccag tattatca
//
LOCUS       DATAFW010000078        15688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_741091, whole genome shotgun sequence.
ACCESSION   DATAFW010000078 DATAFW010000000
VERSION     DATAFW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 15688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15688
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741091"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..819)
                     /locus_tag="VHJ57_01830"
     CDS             complement(142..819)
                     /locus_tag="VHJ57_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX2487306.1"
                     /translation="MDGINDILTFFDFGYEEYGNVGRYFGILIITFFASIILFIPVPY
                     FPVLFTAGINQNLDPHLVSLFSAVGAVIAKLIIFYVSYYGRNILSSKTKKRMLPLQKL
                     LSKYGWPGAFAAALTPIPDDLVYIPLGLAKYNPAKFAIATFSGKFILNEFIVFGSVIL
                     GRPVVEVLTSENTDPIYLYIGLGLSISILILIIFLYLRIDWGKIIGKWFPWTLNNDDN
                     TDNSKTK"
     gene            complement(940..2388)
                     /gene="proS"
                     /locus_tag="VHJ57_01835"
     CDS             complement(940..2388)
                     /gene="proS"
                     /locus_tag="VHJ57_01835"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX2487307.1"
                     /translation="MSELKEIGITTKKEVDFSEWYSQVVTKAGLIDYSPVKGFIVLRP
                     YGYRIWEIIREFLDKKLKESGHQNGFLPILIPEHLLAKEKDHFKGFTPEVFWVTKAGD
                     TDLPEKLAVRPTSETLAYQIFSKWIMSYRDLPLKINFWNSALRAEIKSTKPFIRNSEF
                     LWQEGHTVHENQKDAEDEVLLIFNYYHYLLETILAIPTLKGRKTDKEKFVGALYTNTL
                     ESMMPDGKALQMATSHNLGQNFSKPFEIRFLGKDSKQYFAWQTSWGMSWRVIGALIMI
                     HGDNKGLILPPPIAPTQVVIVPIFKQKERDRVIEESKKLLVELNNLGFRAIVDDREGY
                     TAGWKFNDWEVKGVPIRINIGLRDINNKNVEIVRRDTGEKILIKRIDCSKALNFYLQQ
                     IQTSLLEKAKKMHDANIIKISNYAEMKKILNEKGGFIVANWCEKEECENSVKNETGAD
                     IRLVPFEDYSSDNKCIYCNKHSKKTVIFGKAY"
     gene            complement(2416..2718)
                     /locus_tag="VHJ57_01840"
     CDS             complement(2416..2718)
                     /locus_tag="VHJ57_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487308.1"
                     /translation="MSEPNSISDTNIKFIVPIKLSDKSMRLSDEVKNELKSIGMMDEK
                     GKLKLKGVSPKMLKRMKMESVDCPVLQREISFIPCYVCKNFHSRITGQVYCRGEPL"
     gene            2808..4013
                     /gene="cofG"
                     /locus_tag="VHJ57_01845"
     CDS             2808..4013
                     /gene="cofG"
                     /locus_tag="VHJ57_01845"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HEX2487309.1"
                     /translation="MNSEISFSMKEGSIFEILEGYQNGTTISKDKALDLLESNDILSL
                     MNLAACLKTRFKKDIITYSRKIFINITNLCRDFCSYCTYRKNPFESSSIMMHPTEVLN
                     LAKIGQIYHCTEALIVAGERPEDRYPEAKRWLRQLGYSSLVEYVIDISEKIVKETGLL
                     PHSNLGNITEEEMKLLKTYNVSLGVMLESSSEKLLEKGMPHEFAPSKNPIKRIEVIKN
                     AGRMKIPLTTGLLIGIGETKEELINDLFLLKSINNEYGNIQEIILQNFHPKKDTRMQD
                     CPSPSYDYFLKIVSIARIIMPDMNIQIPPNLSPRTFSSFIDAGINDWGGISPVTIDYV
                     NPEFLWPKIKELKCITESKHHRLRGRLPVYPEFIDKPDFISSDLRNYVENFIDLDGLV
                     KEEYLVEFR"
     gene            4000..5244
                     /gene="cofH"
                     /locus_tag="VHJ57_01850"
     CDS             4000..5244
                     /gene="cofH"
                     /locus_tag="VHJ57_01850"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEX2487310.1"
                     /translation="MNLDSIIKNIDPIISDILNRVIDGNQILADEALELFNANGIELN
                     MVVIVANALCKRKKREIVTYVINRNINFTNVCIKRCGFCAFSRDFREEEGYYLPLEEI
                     VRRAKEAEKLGATEVCIQAGLPPRMDGMLYADICSAIKRELPNIHIHAFSPEEILYGA
                     KRANTSIEEYLKILKDAGLGSIPGTAAEILDQEIRDKISPGRIGVEDWIKVVKCAHSM
                     GINSTSTIMYGHIESNTHITKHLNILRDIQKETRGFTEFVPLSFVHNEAPMYNKNLID
                     GIRNGPTGVEVLKMHAIARIFLNDQIDNIQVSWVKEGPRMGQILLQAGANDFGGTLIN
                     ESISTSAGASFGQLLKPKVIRNIIHNVGKIPAQRKSNYQIIKTFSNDDYFDDEKLDTS
                     NPLDFGSYKELIKMDRFRFKKC"
     gene            5350..7029
                     /locus_tag="VHJ57_01855"
     CDS             5350..7029
                     /locus_tag="VHJ57_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEX2487311.1"
                     /translation="MSNTIEKTFKWKTLEHNGVAFPSNHILKGISIKINNERLDLNTE
                     QEEIVYAWAKKKDTHYIKDTLFQHNFLNDFKKILPSKFQNTNDINQIDFSDAFNLVDT
                     ELKLKIIEKEKMQKLSREEKKKLINKKKQEREKLKNFYGKAYVDGFEVEVANWLVEPP
                     GLFMGRGLHPLRGRWKPRVTPQDVILNLGDDAPVPEGTWKNIVHDHSSTWLATWTEKL
                     TDKRKYVWLHDSAFLRQNTDKEKYDKARNLEKYANKIHIEIINKMINSKDLNQRKIAT
                     VCYLILKLAMRVGDEKDKDEADTVGASTLRIEHIKFPEKDNKKFIEFNFLGKDSVPWQ
                     KTLEINSEDTKGLYHNILLFMKDKEHNSEIFDNINSSKVNAFLRKIDPKNVPGLTAKV
                     FRTYLATRIVKESLKNPYIDVNTHSSEPKKIYVAKIANLKAAIACNHKKGIDPNNPAS
                     KKALEKFEESITKKEDAIEKIRKDILSNKWKTEKQKERLRYRMEKIQYQLKLQKETRD
                     YNLGTSLRNYIDPRIMKSWLDDVELDWKKVYPSTLQRKFKWVESFKKRSTS"
     gene            7184..7258
                     /locus_tag="VHJ57_01860"
     tRNA            7184..7258
                     /locus_tag="VHJ57_01860"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7219..7221,aa:Met,seq:cat)
     gene            8537..9217
                     /locus_tag="VHJ57_01865"
     CDS             8537..9217
                     /locus_tag="VHJ57_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX2487312.1"
                     /translation="MMSERMEPKSKIFVLLILLFVVLSFLVTVNATNNIDNFITDFFY
                     HLGGDPSLDIFMIIVSSSGDLFTMVIVGMILTIIRRTRKIGLIFLISIVVLSISIMYL
                     KPLINRPSPLEQYVPQYNIPDNYTIENDSMSPLSRDLSFPSNHVARDTAFIIIIGYWF
                     KFFKKNTWIGTLLWIFPVLIGISRLYLLQNYFIDILGGALYGIIIAIFLINVLKLNES
                     FLTKKFNK"
     gene            complement(9241..9555)
                     /locus_tag="VHJ57_01870"
     CDS             complement(9241..9555)
                     /locus_tag="VHJ57_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487313.1"
                     /translation="MSSASYWRDLPVYKTLLLEKLEQVKSVCDCMILDYMHFEQTNGF
                     SYRILLPRNKENNQLAKKLGLSLQLEFTSQLRKRKLKTKMKEIRYIHDTKHFGWIITN
                     CE"
     gene            complement(9604..10032)
                     /locus_tag="VHJ57_01875"
     CDS             complement(9604..10032)
                     /locus_tag="VHJ57_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX2487314.1"
                     /translation="MSDDIYREIILDHYRNPRNKGKIDNADVVIHDSNPLCGDQIDIY
                     LKVVQGQIKDIKFDGKGCAISQASASMLTEMVMDKPLTTAKDISKDEVLENIGLTNLG
                     PARIKCALLSLKVLKMGMVKYYSDKEPTTAEKMEKDSNLL"
     gene            complement(10029..11258)
                     /locus_tag="VHJ57_01880"
     CDS             complement(10029..11258)
                     /locus_tag="VHJ57_01880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX2487315.1"
                     /translation="METNKIRNDFPILNRKINGKPFVYLDNASTTQKPSSVIDTIVKY
                     YSFYNSNIHRAVYDIAEEATHAYELTRQKVCKFINANSPQEIIFTRNATEAINLVAYA
                     WGRKNIQNGDIIILSEQEHHSNIVPWQILAKEKNAKIKYLKIDETGNINLNELESYLD
                     EDNGIKLISLCHMSNALGNIIPIDKIIDMAHAYSIPVFVDGAQSVPHLPTDVTSLDCD
                     FLAFSAHKMLGPTGVGILYGKKNILQEMDPFNCGGDMIKEVHKENTLFNDLPYKFEAG
                     TPNIADVIGFSSALDYLNEIGMDNIRKHEIELTKYAIEKMSEFKQLKVYGDINLQNRG
                     GIISFNLGDIHPHDLATILNEHSIAIRSGHHCAQVLMEKLEVSATSRVSFYIYNTKDE
                     INIFINALGKAIKLFQL"
     gene            complement(11365..11808)
                     /locus_tag="VHJ57_01885"
     CDS             complement(11365..11808)
                     /locus_tag="VHJ57_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX2487316.1"
                     /translation="MKIYKNPIPTVDAIIHRNSSILLVKRRRDPYKNCFALPGGFVNE
                     GETIEEAIIREVYEETSLEVYPIDILGVYSDPKRDPRGHMLTVVFIVLIVKGNPSAGD
                     DAQKISWIPINKINEIEIAFDHKLLIEDFLKWKKKGGTYWTNKRR"
     gene            complement(12240..13046)
                     /locus_tag="VHJ57_01890"
     CDS             complement(12240..13046)
                     /locus_tag="VHJ57_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2487317.1"
                     /translation="MLKNKLLINGISVSYFVHNKSIKAVDNVFIELKKGESLGIAGES
                     GCGKTTLVSAIMRNITPPGKITAGSIFLDGEEITKLSDKDFDSRIRWKKISLVFQGAM
                     NSLDPVYTIKNQINDIFETHGQSYDKEKVITILKNVGLDKSVLGQYPHELSGGMKQRI
                     VIALALLLQPDFIIADEPTTALDVLVQAQIIKLLKNLKENGISIILISHDLALISELV
                     DKIGIMYAGKIVEFNLTKEIIFSPKNEYTKKLILSTPQLGQTFKKINQSK"
     gene            13147..13653
                     /locus_tag="VHJ57_01895"
     CDS             13147..13653
                     /locus_tag="VHJ57_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487318.1"
                     /translation="MKTPICSFDAKTGILCSKCESKLKSGHLSIGDIEAAMKLTKLAD
                     KNNIIDKFTLVGAHKIDEELILILPTSDISILRSNMDLLDYIKKEFNNSVWFIESGAT
                     ERRFIENLIFPVKIVSINLVWLPDGNKLTKIIISKKKNINANIDTKLITKIAEEIRKI
                     ELLVEYID"
     gene            13707..14999
                     /gene="aspS"
                     /locus_tag="VHJ57_01900"
     CDS             13707..14999
                     /gene="aspS"
                     /locus_tag="VHJ57_01900"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX2487319.1"
                     /translation="MKEKIKRTHFANELKENILGEKVKVGGWIEDVRDIGKIIFLVIK
                     DITGSIQVIVDPEEFPQIREIPRQSSILVSGKIQKSKAKEMTVELKLEEVVAIYLSKH
                     PLPIDPTGRLESSLDKRLDARALDLRNENVSKLFVIRSIALQIVRKTLLEMAFLEVNT
                     PKIIGSASEGGANLFEFDYFKNKAYLAQSPQLYKEQLVLALDRVFEIAPYFRAEKSNT
                     VRHLTEFYSVDIEAAFLDHYDIMEIMERVIKNVIIGIKDSALLDFTTNEFLNKAIETK
                     FPRLTYQTCIEELKENGECVTYGDDLSDSSLRKLGRLHPGFYFITEWPLKLKPFYILE
                     NKRDPFLSDSFDLQIGYLELISGGSRQHDPTKIRNRLAEQGLNPDKFSEHLKVFDWGM
                     PPHAGCGLGLDRLIMSLTGSMNVREVVLYPRDPYRISP"
     gene            complement(14989..15408)
                     /locus_tag="VHJ57_01905"
     CDS             complement(14989..15408)
                     /locus_tag="VHJ57_01905"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX2487320.1"
                     /translation="MYNPNISIHHKHLNALAKLAISTLPNESCAILLGKRSSIKPVVH
                     YILPMNNSSNSSTEFNIEADDLYYAYNKARLMNLDIIAIFHSHPSQPIPSDMDKTFML
                     INPIVWIIYSTLSHTFKAFILDQTDQLQEISIHLIKD"
BASE COUNT         5652 a         2285 c         2434 g         5317 t
ORIGIN      
        1 ttttcatcca caaataatgg aaaaatcata tcctttttat ataaactaca tctttttgaa
       61 aattcataat aattatattc atagtctacc atgtttttct tccttttaac tatattttat
      121 ttttcattcc ataggatcga atcatttagt ttttgaatta tccgtgttgt catcattgtt
      181 taatgtccaa ggaaaccatt taccaataat ctttccccaa tcaattcgaa gatacaaaaa
      241 aataattagt atcaatatac taatactcaa tcccagacct atatagagat aaataggatc
      301 tgtgttttct gaggtcaaca cttctacaac aggtctgcct aaaatgacac taccaaatac
      361 aataaattca ttcagaatga attttcctga aaaagtagct atcgcaaatt tggctggatt
      421 atattttgct aagccaagtg gaatgtaaac aagatcatca ggaattggag tcaatgctgc
      481 agcaaacgcg cccggccaac catattttga taaaagtttc tggagaggaa gcatcctttt
      541 ttttgtttta gagcttaata tatttcgacc ataatagctg acataaaaaa ttattaattt
      601 agctatgact gctcctactg cactaaaaag agaaactaaa tgaggatcaa gattctggtt
      661 aataccagca gtaaataata ctgggaaata aggaacaggg ataaataaaa ttatactagc
      721 gaaaaaagta atgattaaga ttccaaaata tcttcctacg tttccatatt cctcatatcc
      781 gaagtcaaaa aaagtaagta tatcattaat gccatccaaa tattgagact cctatctgtt
      841 tatattttgt gtaatttaag tcaataatta tccagaaata tccaggaagt ggaattataa
      901 tttacaggaa tcatattaga gttttaacta gtaaattagt taatatgctt taccaaaaat
      961 tactgtcttt ttagaatgct tattacaata tatacatttg ttatctgaac tataatcctc
     1021 aaatggaaca agacgaatat cagcacctgt ctcatttttt acgctatttt cacattcttc
     1081 tttttcacac caatttgcta caataaaacc gcctttttca tttaatatct tcttcatttc
     1141 ggcataatta gaaatcttta ttatattagc gtcatgcatt ttcttagcct tttcaagtaa
     1201 ggatgtttgg atttgttgta gataaaaatt tagagcctta ctacaatcta ttcttttaat
     1261 tagaattttt tctcccgtat ctcttcttac aatctctaca tttttattat taatgtctct
     1321 aagtccaata ttaattcgaa tcggtactcc tttaacttcc caatcgttga atttccatcc
     1381 tgctgtatat ccttctctgt catcaacaat agccctgaac cccaaattat ttagttcaac
     1441 caatagcttt ttagattctt caataactct atctcgttct ttttgcttga atattgggac
     1501 tatcaccact tgagtaggag caattggagg aggtaaaatt aagcctttat tgtcaccatg
     1561 aatcataatc aaggctccta ttactctcca tgacattccc caggatgttt gccaagcaaa
     1621 atactgtttt gagtctttac ccagaaaccg aatttcaaat ggcttggaaa aattttgacc
     1681 tagattatga gaagtagcca tttgtaatgc ttttccatct ggcatcattg attcgagtgt
     1741 gttagtatag agggctccaa caaatttttc tttatctgtt ttgcgtcctt tgagagttgg
     1801 gatagccaaa attgtttcta aaaggtaatg gtagtaatta aagatcaata atacttcatc
     1861 ttcagcatct ttttgattct catgaacagt atgtccttct tgccaaagaa attccgagtt
     1921 tctaataaaa ggtttagtag atttaatttc agcacgtaat gctgaattcc aaaaattgat
     1981 tttcaatgga aggtccctat aactcattat ccatttagaa aatatttgat aggcgagagt
     2041 ctctgaagtg ggtcgtaccg ctaacttttc aggtaagtca gtatctcctg ccttcgtcac
     2101 ccaaaagacc tcaggagtaa atcctttaaa atggtccttc tctttagcta agaggtgttc
     2161 tgggataaga attggtagaa acccattttg atgtccggat tcttttaatt ttttatctaa
     2221 aaattccctt ataatttccc agatcctata tccatagggt ctaagtacaa tgaatccttt
     2281 tacgggcgaa taatctatta gcccagcttt ggtaactact tgagaatacc attcactaaa
     2341 atcgacctct tttttagttg ttatcccaat ctcttttaat tctgacaaaa aaatccctaa
     2401 ttttaaaggt aatgattata atggctcacc acgacaatat acttgtccag ttatcctact
     2461 atgaaaattc ttacaaacgt aacatggaat aaacgatatt tctctttgaa gaacaggaca
     2521 atctaccgac tccattttca ttctttttag cattttagga ctaacacctt ttagtttaag
     2581 ttttcctttt tcatccatca ttccaatact ctttaattca tttttgacct catcgctaag
     2641 tcgcatcgat ttatctgata acttaattgg tacaataaat tttatattgg tatcagatat
     2701 agaattaggt tcactcatgt atttagattc taatgatata atatatgatg tttcctatat
     2761 cataaaaaaa ttaaagataa attactttac taattgatat aatagtgatg aattcagaaa
     2821 tttcattttc aatgaaagaa ggctcaatat ttgaaattct ggaaggatat caaaatggaa
     2881 ctactatttc taaagataag gccctcgatc ttttggagtc taatgacatt ctatctctaa
     2941 tgaatcttgc agcatgttta aaaactaggt ttaaaaaaga tattattact tattctagga
     3001 aaatctttat taatataact aatttatgta gggatttttg ttcatattgt acttatagaa
     3061 aaaatccatt tgaatcatct tcaattatga tgcacccgac cgaagtactt aatttagcaa
     3121 aaattggaca aatatatcac tgtactgaag ctttaattgt tgcaggggaa agaccagaag
     3181 atcgatatcc agaagcaaaa agatggctaa ggcaactggg ctattcttct ctagtagaat
     3241 atgtaattga tatttctgaa aaaattgtaa aggagactgg cttactccct cattccaatc
     3301 tgggaaatat taccgaagag gaaatgaaat tattaaagac atataatgta agcctaggtg
     3361 taatgctcga atcgtcaagt gagaaattgc tagaaaaagg tatgcctcac gaatttgcac
     3421 ctagtaaaaa cccaattaag agaattgagg taataaagaa tgctggcaga atgaaaatac
     3481 ctttaaccac agggctatta attgggattg gggaaacaaa agaagaacta ataaatgatt
     3541 tgtttctttt aaagagtatt aataacgaat atggaaacat tcaggaaata attttacaga
     3601 attttcatcc taaaaaagat actagaatgc aagattgtcc ttctcctagt tatgattatt
     3661 tcttaaaaat agtgtctatt gccagaataa taatgcccga tatgaatatt caaattcctc
     3721 caaacctatc tcccagaact tttagttctt tcatagatgc agggataaat gattggggag
     3781 gaatttcgcc tgtaacaatc gattatgtaa atcctgaatt tctctggcca aagataaaag
     3841 aactaaaatg tattactgaa tcaaaacatc accggcttcg gggaaggtta ccagtctatc
     3901 ctgagtttat agacaaacct gactttattt cttctgattt acgaaattat gtagaaaact
     3961 ttattgactt agatggatta gtgaaagagg aatatttagt tgaatttaga tagtataata
     4021 aaaaatattg atccgattat ttctgatata ttgaatagag taattgacgg aaatcagata
     4081 ttagcagatg aagctttaga actgttcaat gctaacggaa tagaattaaa tatggttgta
     4141 attgttgcta acgctctttg taaaaggaaa aaaagagaaa tcgtaacata tgtcataaat
     4201 cgaaatataa attttacaaa tgtttgtatc aaaagatgtg gtttttgtgc atttagtaga
     4261 gactttaggg aggaggaggg gtactattta cctcttgaag aaatagtaag aagagcaaaa
     4321 gaggctgaaa aattgggtgc gactgaagta tgtattcaag caggccttcc cccacgaatg
     4381 gacggcatgt tatatgccga tatatgtagt gcaataaaaa gagagcttcc aaatatccat
     4441 attcatgctt tttcacctga ggaaattcta tacggtgcaa agagagccaa tacctcaata
     4501 gaagaatatt taaaaatact taaagatgca ggtttaggaa gtattcctgg aactgctgct
     4561 gaaatattag atcaggaaat aagagataaa atttctccgg gaagaatagg cgtagaagat
     4621 tggattaaag ttgttaaatg tgctcattca atgggaatta attctacttc tactattatg
     4681 tatggtcata tagaaagcaa tacccatatt actaaacatt tgaatatact gagagatatt
     4741 caaaaagaaa ctagaggctt taccgaattt gtgcctttga gttttgtaca taacgaggcc
     4801 ccaatgtata ataaaaatct tattgacggt attaggaatg gacctactgg agtcgaagta
     4861 ttaaaaatgc atgcaattgc aagaattttt ttaaatgacc aaatcgacaa tattcaagtt
     4921 tcatgggtaa aagaaggacc acgaatggga cagattttat tacaagcagg tgctaacgat
     4981 tttggtggaa cactaatcaa cgaaagcatt tctacgtctg ctggagcctc ttttggacag
     5041 ttattaaaac caaaggttat tagaaatata atacataatg tgggcaaaat ccccgctcaa
     5101 aggaaatcaa attatcaaat tatcaaaaca ttttctaatg atgattattt tgacgatgaa
     5161 aagcttgata catctaatcc actggatttt ggatcatata aagaattaat aaaaatggat
     5221 cgctttaggt tcaaaaaatg ttgaacgata ttcattttct tgtaaatttt aggtatacac
     5281 cttttttaaa ttgttttatt ggtaagattt atcaatagta aaacatgacc attaaatttg
     5341 tatctattaa tgtcaaatac cattgagaag acgtttaaat ggaaaacatt ggaacacaat
     5401 ggagtagctt ttccttctaa tcatatttta aaaggcataa gtattaaaat aaataatgaa
     5461 agattagatc ttaacactga acaagaagaa atagtatatg catgggcaaa aaagaaagat
     5521 actcactata ttaaagatac tcttttccaa cataatttcc tgaatgattt taaaaaaata
     5581 ttaccaagta aattccagaa tacaaatgac ataaatcaaa ttgatttttc agacgcattt
     5641 aaccttgttg atacggaact taaactcaaa ataatcgaaa aagagaaaat gcaaaagtta
     5701 agtagagaag agaaaaaaaa gttaataaat aaaaaaaaac aagaaagaga aaagctaaag
     5761 aatttttatg gaaaagcata tgttgatggg tttgaagttg aagttgccaa ttggttagtt
     5821 gagcctccag gtttattcat gggtagaggc ttacatccac ttagagggag atggaaacca
     5881 agagtaacac ctcaagatgt tatattgaat ttgggagatg atgcacctgt tcctgaagga
     5941 acttggaaaa atattgttca cgatcattca tcaacttggt tagcaacatg gacggaaaaa
     6001 ttaactgata agagaaaata tgtttggtta catgattcag catttctcag acaaaatact
     6061 gataaagaaa aatacgacaa agcgagaaat ctcgaaaaat atgccaataa gattcacatc
     6121 gaaattataa ataagatgat aaattctaag gatttaaacc aacggaaaat cgcaactgtt
     6181 tgttatttaa tacttaaatt agcaatgaga gtaggcgatg aaaaagataa agatgaggca
     6241 gatacagttg gtgcgtctac attaagaata gaacacataa aatttcctga aaaagataac
     6301 aagaaattca tagaatttaa ttttctggga aaagatagtg tcccatggca aaaaacgtta
     6361 gaaataaatt ctgaagatac aaaaggtttg tatcataata ttctattatt tatgaaagat
     6421 aaagaacata atagtgaaat ttttgataat ataaattctt caaaggtaaa tgcatttcta
     6481 agaaaaatag acccaaaaaa tgtcccagga ctaactgcaa aggtatttag gacatatctt
     6541 gcaacaagaa tagtgaaaga atcattaaaa aatccctata ttgatgttaa tacccattca
     6601 tcagaaccta aaaaaattta tgtggccaaa attgcaaatt taaaagcagc aattgcatgt
     6661 aaccataaaa aaggaattga tcccaataat cctgccagta aaaaggcatt agaaaaattt
     6721 gaagaatcaa taaccaaaaa ggaagatgct attgaaaaaa taagaaagga tatattgtct
     6781 aataaatgga aaacagaaaa acagaaagaa agactaagat atagaatgga gaagattcag
     6841 tatcaattaa aactacaaaa agaaacacgc gattataatt taggtacttc tttaagaaat
     6901 tatattgatc cgagaattat gaaaagctgg ttagatgatg tagaattaga ttggaaaaag
     6961 gtgtatccgt cgacgttgca aagaaaattc aaatgggttg aatcatttaa gaaaagatca
     7021 acttcttaga atcgtcaaat ttttaaaaaa aataattaaa atataaatac aaaaattgtt
     7081 atgctttttt ataccatata ttatcgaaag ttaaaggaga aattcattat taatagaata
     7141 tatatcacaa ctgaaataac aggaatgaaa cgctgctgag catgccggag tggctcagct
     7201 tggttagagc gttcgactca taatcgaaag gtcgcgggct cgaatcccgc ctccggcatt
     7261 aaattctctt aatttcaatc tccccaaaaa aaatatgaaa aagtataaac acacagaaat
     7321 ttaaaatctg aagagggtga tagaattgga atgactccca cgaattacaa aattacattt
     7381 taaaataaac tacgaactat agataaaaag agtaataaat agtaaataca ggtcagacag
     7441 taggaactgg atattaaata cgtaggtagt ctacaatatg gtatgatcaa atggaaaaag
     7501 atatgaaaga ttttgaagta gggtttaatt atgtagaaat tagatgagct gacagttcaa
     7561 tacctaagcc atttatagag atgttgaaca atcccagatt ccattaaatt cataatttaa
     7621 cataagaatg agagatgaga aggaggtagt attaatctac aaaaaatcta tagataatta
     7681 aaatcttaca aagaataatt atctgatgat gtattatctg cacaatttgc atctatttta
     7741 aaaaatgtaa gatactaatt atatagaaca agaaaattct ttatttagaa tattctcggc
     7801 gctcgcaaaa aaaaattcca ttacaaatgc ataaaatggg ttttcggtgg tttcggtatt
     7861 gtttttatta gtatgttatt gatcttgcta atccatgatg tcctgactgg tgaatcttcg
     7921 aagacaacat aatattgtac caagtatggt tttttagcat cctctgattg ttggtgatat
     7981 tgaattggtg taatttgccg gctaacatct agtgtgttaa ctgcttcaag aaacacagtt
     8041 ttatgtaaat attaaaaata taatatagaa atactgtaga ttctttcata actttgaggt
     8101 ctgaaaactg ttatccacgg aatttttcat aaaaaaccag gtatttttga aagaaacatt
     8161 ccgaagagaa tgtcctaatg gatggtaata cacataagca aaaatagcta taacgtacta
     8221 cgactaggca gtgtgtgata gcagtattcg gactattgct ggcaggatac tacatattta
     8281 gaatacatta ataacttaat tcttattcgt accattaaga gataactttc cactcttact
     8341 aatagcgtaa gattagcaag aaattatatt tatccttaaa ataacaatta attttcatat
     8401 ttacaaattt tgtaaatact attattttct ttacttggta aatagcttaa ttcttatcga
     8461 aagtcaaaga tcaagtaact tagagaaatt gaaaggttat attatttata taactggata
     8521 agtggatacc gaaaatatga tgagtgaaag gatggaacca aaaagtaaaa tatttgtatt
     8581 attaatcttg ctatttgttg ttttgtcttt tttagttacc gttaatgcta caaataacat
     8641 tgacaatttt ataactgatt ttttttatca cttaggtggg gatcctagcc tggatatatt
     8701 tatgattatt gtttcttcat ccggtgactt gtttactatg gtgattgttg gaatgatttt
     8761 gactataata agaagaacga gaaagatagg attaattttt ctaatttcaa ttgtagttct
     8821 atctatatca ataatgtatc tgaaaccgct tataaatcga ccatctccac ttgaacagta
     8881 tgttccacag tataatattc ctgataacta tacgattgaa aatgatagta tgtcacctct
     8941 atcaagagat ttatcttttc catctaacca tgtggcacgt gatactgctt tcataataat
     9001 cataggatat tggtttaagt tctttaaaaa aaatacatgg attggaactt tattatggat
     9061 ttttcctgta ttgataggca ttagtagact atatctactt caaaattatt ttattgacat
     9121 attgggaggg gcactatatg gtataataat tgccatattt ctcataaatg tattgaaatt
     9181 aaatgaatca tttcttacaa aaaaatttaa caaataggat tgctcatatg tggtttttgt
     9241 tcactcacag ttagttataa tccatccaaa gtgttttgta tcatgtatat acctaatttc
     9301 tttcatctta gtttttaatt tccgttttct taattgagaa gtaaattcta attgaagact
     9361 aagacccagt tttttggcta attgattatt ttctttattt cttggtaaaa gaattctata
     9421 actaaagcca tttgtctgtt caaagtgcat ataatccagg atcatgcagt cacaaacaga
     9481 tttgacttgt tctaattttt ctaaaagaag agttttatat actggtaaat ctctccaata
     9541 agaagcactt gacatatagg tttactatat ttcaaaaata ttattattta aattaaaact
     9601 aaatcataaa aggtttgaat ccttttccat tttttcagct gttgttggtt ctttatcaga
     9661 ataatatttt accataccca tctttaagac cttaagagat agtaatgcac atttaatcct
     9721 tgcagggcct aaattggtca accctatatt ttctaatact tcatctttgg agatatcttt
     9781 agcagtagtt aaaggtttat ccatcaccat ctctgttagc atagatgcac tagcttggct
     9841 aattgcacac cctttgccat cgaatttaat atctttgatt tgcccttgta ctactttgag
     9901 atatatatca atttgatcac cacataaagg attgctatca tgaatgacaa cgtctgcatt
     9961 gtcaatcttt cccttatttc taggatttct ataatgatct agaataattt ctctataaat
    10021 atcatcactc aaagttgaaa cagtttaata gctttaccta acgcattaat aaagatattg
    10081 atctcatctt ttgtattata gatatagaaa ctaaccctag atgtagcgga aacctcaagc
    10141 ttttccatta atacttgagc acagtggtga ccagagcgaa tagctataga gtgctcattt
    10201 aaaatagttg ccaaatcatg aggatgaata tcacccaaat taaacgagat aattccacct
    10261 cgattttgta aattaatatc tccatatact tttaattgtt taaattctga cattttttca
    10321 attgcgtatt ttgttaactc gatttcatgc tttctgatat tatccatccc tatttcgttt
    10381 agataatcca aagcagaact aaaaccaata acatcagcga tattaggagt accagcttca
    10441 aatttataag gtaaatcgtt aaagagtgtg ttttccttat gtacttcttt aatcatatct
    10501 ccaccacaat taaatgggtc catttcctgt aggatatttt ttttcccata caagattcct
    10561 actcctgttg gtcctaacat tttatgagca gaaaaagcaa gaaagtcaca atcaagagaa
    10621 gtaacatcag ttggtaaatg aggaacagac tgggcaccat caacaaatac tggtatacta
    10681 tatgcatgtg ccatatctat aattttgtct attggtataa tattaccaag tgcattagac
    10741 atatgacata atgaaattaa ttttatacca ttatcttcat caagataaga ttctaattca
    10801 tttaaattta tgtttcctgt ttcatcaatt tttaaatatt ttatttttgc gtttttttct
    10861 ttagctaata tttgccaagg aacgatatta ctatgatgtt cttgttcaga taaaattatt
    10921 atatctccat tttgaatatt ttttcgaccc caagcgtatg ccactaaatt aattgcttct
    10981 gtagcattac gagtaaaaat tatttcttga ggagaatttg cattaataaa cttacaaacc
    11041 ttttgtctcg ttaattcata agcatgagta gcttcttcgg ctatatcata aacggctcta
    11101 tgaatattag agttgtaaaa tgagtaatat tttacaattg tatctatcac tgatgaaggt
    11161 ttttgggtag ttgaggcatt atcaagatag acgaaaggct taccattaat tttacgattt
    11221 agaattggaa aatcattacg tattttattt gtttccaatt cggagaattg cataattaat
    11281 tattactaat tagtaaatat aaatggattg aagggaaatg aaaatttttc agatttcttt
    11341 gtaaatacaa atgatctttg atcattacct tctcttgttc gtccaataag ttccgccttt
    11401 ttttttccat ttcaaaaaat cttctataag aagtttatga tcaaatgcaa tttctatttc
    11461 attgatttta tttattggaa tccatgaaat tttctgggca tcatcaccag cactaggatt
    11521 acctttaaca attaaaacaa taaatacaac agttagcatg tgtccccttg gatctctttt
    11581 aggatcagaa tagactccca aaatatctat agggtagacc tctaatgatg tctcttcata
    11641 tacttctctt ataatagcct cctctatagt ctctccttca tttacaaaac ctcctggtaa
    11701 tgcaaaacaa tttttatacg gatctctcct acgtttgact aataatatcg aagaattcct
    11761 atgaataata gcatcaacag tagggatagg atttttgtat attttcaagt gaatctttca
    11821 ttgacaattg aaaataatat atatgtttaa tttacttctt aatttatgca gttgttatgg
    11881 agggaacctt ggttaaaacc tctaatagat tacgatattg attctgaaag ttgtagcttt
    11941 tgtaaaaaaa aatttaatag tttatcattt gttacatcat gtgaattttg tcaaatagaa
    12001 attatgcatg acaaatgtgc taattcacat atattagaac gccatatgtc tgaattaaaa
    12061 acaaaaattg gtcaacataa agaaaaagcc ttacatgatt tccaataaaa tatcttttta
    12121 tttgtaatat attaagcatc tacaattttt tatctggcaa tacttttaga atataattct
    12181 atggaattaa ttcaaaatac atttactttc atattaaatt cgaaacaaaa agaaattttt
    12241 tatttacttt gatttatttt tttaaaagtt tgtcctaatt gaggagtaga taatatgagt
    12301 tttttagtat actcattttt gggagaaaat ataatttctt tggttaaatt aaattcaaca
    12361 atttttcctg catacataat tcctattttg tctaccaact cagaaattaa agcgagatca
    12421 tgagaaatta aaataatact gattccattt tctttaaggt tttttaataa cttaattatt
    12481 tgtgcctgaa caagcacatc taaagctgta gttggttcat cagcaatgat aaaatcaggt
    12541 tgtagtaata aagctaaagc tataactatt cgttgtttca tacctccact taactcatga
    12601 ggatattgtc ctagaacaga tttgtctagg cctacattct ttagaattgt tattactttt
    12661 tctttatcat aagattgacc gtgagtttca aatatatcat taatttggtt ttttattgta
    12721 tatacaggat ctaatgaatt catggctcct tgaaaaacta aagaaatttt tttccatcta
    12781 atcctcgaat caaagtcttt atcggataac tttgttattt cttctccatc tagaaaaata
    12841 cttcctgctg taatttttcc aggaggtgtt atatttctca ttattgcaga aaccaatgtg
    12901 gttttaccac aacctgattc tccagcaatg ccaagtgatt cacctttttt taattcgata
    12961 aatacattgt caactgcctt tatactttta ttatgtacaa agtaagagac acttataccg
    13021 tttataagta atttattttt taacaaatct atttattatc taacctaaat aaatatattt
    13081 ttcatccttt tatcatttta gctctctata tatatatgga tcagtagcat taatgagtga
    13141 gaattattga aaactcctat ttgttcattt gatgctaaaa caggaatttt atgttcaaaa
    13201 tgtgaatcta aattaaaaag tggtcatctg tcaatcggag atattgaagc tgctatgaaa
    13261 ttaactaaat tagctgataa aaataatata attgataaat ttacactagt tggagctcac
    13321 aaaattgatg aagaattgat attgatactg ccaacgtcgg atattagtat attacgttcg
    13381 aatatggact tattagatta tataaaaaag gaatttaata atagtgtctg gttcattgaa
    13441 agcggtgcca cagaaaggag atttattgaa aatttaatat ttccagtgaa gattgtttca
    13501 atcaatttag tttggctgcc tgatggaaat aaacttacaa aaataataat atccaaaaag
    13561 aaaaatatca atgctaacat agataccaaa ctcattacta aaattgcaga agaaattaga
    13621 aagatagaat tgcttgtcga gtatatagat taaatatata agaatatgga aaaaattgaa
    13681 ataaaccata taagatatta attcaattga aagaaaaaat taagagaacg cattttgcta
    13741 atgaattaaa agaaaatata cttggagaga aagtaaaagt cggtggctgg attgaagatg
    13801 tacgtgacat tggaaaaata atatttttag taattaaaga tataactggt tcaattcaag
    13861 tcatagttga tccagaggag tttccacaaa taagagaaat acctagacaa agctctatct
    13921 tagtttctgg caagatacaa aaaagtaaag caaaagaaat gacagtagag ttaaaattag
    13981 aagaggttgt cgccatttat ctttctaagc atcccctccc aattgatccg actggccgat
    14041 tagaatcatc tcttgataag agactcgatg caagagcatt agacttaaga aatgaaaatg
    14101 tttcaaaatt atttgttata aggtctattg ctttacaaat tgttagaaaa actttactag
    14161 agatggcttt tctcgaggta aatactccga agattatagg aagtgctagc gaaggaggtg
    14221 caaatttatt tgaatttgat tactttaaaa ataaagcata tcttgcgcaa agccctcagc
    14281 tttataaaga acagcttgta ttggcattgg atcgtgtatt tgaaatagca ccatatttta
    14341 gagcagagaa atctaatact gtaaggcatt taacagaatt ttatagtgtt gatattgaag
    14401 ccgctttttt agaccattac gatataatgg aaataatgga acgagtaata aaaaatgtta
    14461 tcataggtat aaaagattca gctttgctag attttacaac aaatgaattt ctaaataagg
    14521 caattgaaac aaaatttcca agacttacat atcagacatg tatcgaagaa cttaaagaga
    14581 atggtgagtg tgtgacgtat ggagatgatt tatcagattc gtccttaaga aaattgggtc
    14641 gattacatcc tggtttttat tttataacag aatggccttt aaagttaaag cccttctata
    14701 ttctagaaaa taaacgggat cctttccttt ctgattcctt tgatttacaa attggttatt
    14761 tggagttaat atcaggagga tcacgacaac atgatcccac taaaattaga aatagattag
    14821 cagaacaggg tctgaatcca gataaatttt cagaacactt aaaagttttt gattggggca
    14881 tgccccctca cgctggttgt ggtttaggtc tagatagatt aattatgtct ttaacaggat
    14941 cgatgaatgt aagagaagtt gtattgtatc caagagatcc atatagaatt agtccttaat
    15001 taagtgtata cttatttcct gtaattgatc ggtttgatcc aatataaatg ctttaaaggt
    15061 atgagaaaga gtagaataaa ttatccaaac aataggatta atcaacataa aagttttgtc
    15121 catatctgag ggaataggtt gagaaggatg tgagtggaaa atcgcaataa tatctaaatt
    15181 cattaaccta gctttattat aggcataata tagatcgtcg gcttcaatat taaactcagt
    15241 agacgaattc gaagaattgt tcataggtaa aatataatga acaaccggct tgatacttga
    15301 tcttttacct aacaatattg cgcaggattc attgggtaat gtgctaattg ctaatttcgc
    15361 tagtgcattt aaatgcttat gatggatgga tatatttggg ttatacataa atttagaaag
    15421 aaagtatcct ttatattcat acattaaaaa gaacaaaata atgttattcg ttaatactga
    15481 aataaatatg gataatgtca tatatgttat acaattttgg tatagaaaaa attcaactct
    15541 atttagaaat aactgaataa aattattcta tttgaaattt ggctttcata tatggatgta
    15601 ttgtacaaaa atagtcatat cctttttctt taagatcaga aagcaaaaga gtataattag
    15661 catttcccat aattatagaa gaatcaaa
//
LOCUS       DATAFW010000079         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6204481, whole genome shotgun sequence.
ACCESSION   DATAFW010000079 DATAFW010000000
VERSION     DATAFW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6204481"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VHJ57_01910"
     CDS             <1..291
                     /locus_tag="VHJ57_01910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the B subunit is part of the
                     catalytic core of the ATP synthase complex; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEX2487321.1"
                     /translation="ADQLYATYAQGKDARALSAIVGEEALSETDKKFLKIANNFERKF
                     VNQGTDENRSIEQTLDIGWELLSHLPESEMKRIKSEFILKYRKRVDKESEGA"
     gene            305..970
                     /locus_tag="VHJ57_01915"
     CDS             305..970
                     /locus_tag="VHJ57_01915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEX2487322.1"
                     /translation="MALATNIRPTRLEYIRTKRRIVIARKGLKLLKLKRQALILEFFN
                     TSKTAAALRGTLQSELLKGYETIRLAEMLAGSIRLENEAMKMSQIANLQVMSKSIMGV
                     HIPKIVGGQRELLAEHILELPTSINESIRTFQKIHNTVLEIAEKETTLRKLLYEIERT
                     KRKSNAIENVFIPKLVSALKFITFRLDEMERDTFIMLKTVKRKMGEREHEITKETEVE
                     INV"
     gene            1121..1447
                     /locus_tag="VHJ57_01920"
     CDS             1121..1447
                     /locus_tag="VHJ57_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487323.1"
                     /translation="MSNTQEEYLHSLKRIKEVEDKVRVEIETHRKLIEEKINSLQKDL
                     ENDIAASKVQGERLVEKSIEEALINASKEAEKIMEDSEKKSQTISIHIDQQTIKKIID
                     IFLSGM"
     gene            1464..>3194
                     /locus_tag="VHJ57_01925"
     CDS             1464..>3194
                     /locus_tag="VHJ57_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEX2487324.1"
                     /translation="MSTMLKPAAMAKIAVIGLKKYRPQIISIIHEMNVIQLEHLSKEV
                     DSLLLKEHESDLHKQISDQLLRIRGLLNSLPPSPILEKSKFSSIEELIKTLKSMDIDK
                     IIASLERQKEGILTTIKETENNLKLLEEFSFFPEDLKLLHLSFARSYFGRITLEKFPE
                     FKKTLESNGGPIILYSQTQNNFMYFVLVIPPNFPSNILASAVSLYGVHLETVPKLEGK
                     PRDIIHSQKNLHADLHSQLKLINNQLSEISKANYTFLKGAEEELEIENQKLEVIEDLG
                     VTNDAFALEGWIPRSKIEDLKIAFESYSKGTLLYELETKDNPPTLLVNPKRFKVFESF
                     VRFYSLPSGNEFDPTLIFGLIFPIFYGLMIGDVGYGLVILLVSLWVIRRVQHRKRNLT
                     IMPKFLRNFAKTILRPSQMVKLAKAMIPGCIIAIILGFCFDLYFGFHLNAYLFSFLNN
                     FGLNLPADGALLNPIGTFGLRKLLLISGYIGLGMVSFGLVLGILNSMRERNIKHIISK
                     VGWLLFGWGIALVGLAMINHVNINPLQNIQGAGYFALLFGGIGLMFYGEGIRALMELP
                     SIISHILSYTR"
BASE COUNT         1161 a          464 c          584 g          985 t
ORIGIN      
        1 gctgatcagc tctatgcaac gtatgcccaa ggtaaagatg caagagcatt gtcggcaatt
       61 gtaggagagg aggcattaag tgaaactgat aaaaagtttc ttaaaatcgc caataatttt
      121 gagcgaaaat tcgtcaatca aggcactgat gaaaatagat caatagaaca aactttagat
      181 attgggtggg aattgttaag tcatctccct gaatcagaaa tgaagcgtat aaaatcagaa
      241 tttatcctaa aatatagaaa acgagtcgat aaagaatcgg aaggagcgtg actgtctaga
      301 aaatatggca ttagcaacca atatccgacc aactcgcctt gagtatattc gcaccaagcg
      361 taggattgtt attgcaagga aaggattaaa gcttttaaag ctaaaacgtc aagccttaat
      421 tcttgaattt tttaatacca gcaagactgc tgcggccctt agaggaacct tacaatctga
      481 attgttgaaa gggtatgaaa ctattaggtt ggcggagatg ctcgctggtt caatacgact
      541 agagaatgaa gcaatgaaaa tgtctcaaat tgcaaatttg caggtgatgt caaagagtat
      601 tatgggagtg cacattccaa agatcgtagg aggtcaaaga gaattactgg cagagcatat
      661 actagagctt cctacctcaa taaacgagtc tataaggaca tttcaaaaaa ttcataatac
      721 tgttttagag attgcagaga aagagacaac attaagaaag cttttgtatg agattgagag
      781 gacaaagcgt aaatctaatg caatagaaaa tgtctttatt cctaaattgg tgtctgcatt
      841 gaaatttatt acatttcgcc tcgatgaaat ggaacgggat actttcataa tgctaaagac
      901 cgtaaagaga aagatgggag aaagggaaca tgaaattact aaggagactg aagtagagat
      961 taatgtataa taataataat aataataata aaagagattt tgtaaatgcc cagcaaaaaa
     1021 aattttattt aatagcaaga gcatttaagt tcggaaacat acttgcgaca attatactaa
     1081 tttcatttat aggaaaatat tttctaggat gatttaatat atgtctaata cgcaagaaga
     1141 gtatcttcat tctctaaaac gaattaaaga agttgaggat aaagttcgag tagaaattga
     1201 aacacatcga aaattaattg aagaaaaaat taatagtttg cagaaggatt tagaaaacga
     1261 tattgctgca tcaaaagtcc agggagaaag attagtagaa aaaagtattg aagaggccct
     1321 gattaatgct tccaaagaag ctgaaaagat tatggaagac tcagagaaaa agtcccaaac
     1381 tatatctatt catatagatc aacagacaat taaaaaaata atcgatattt ttctttctgg
     1441 aatgtaatgg atgcgatatt aagatgagta caatgttgaa accagctgca atggctaaaa
     1501 tagcagttat cggactaaaa aaatacagac ctcaaattat atcgatcata catgaaatga
     1561 atgtcattca attagaacat ctttcaaaag aagtagattc attactattg aaggaacatg
     1621 aaagtgattt gcataaacag atttccgatc aattattgcg aattagagga ttacttaatt
     1681 cccttcctcc ttcaccaata ctcgaaaaat ctaaattttc ttcaattgaa gaacttatta
     1741 aaactctaaa gtcgatggat attgataaaa ttattgcttc actggaaagg caaaaagaag
     1801 gaatcctaac aacaattaaa gagacagaaa acaatctaaa gcttttagaa gaattttcat
     1861 tttttcctga agatctaaaa cttttacact tatcttttgc ccgttcatat tttggtcgaa
     1921 ttactttaga aaagtttcca gagtttaaaa aaacattgga atcaaatggc ggacctatta
     1981 tcctatattc acagactcaa aataacttta tgtattttgt gcttgttatt cctccaaatt
     2041 ttccttccaa tattctcgct tcagcagtga gcttatatgg tgtacatttg gaaacagtcc
     2101 caaaactgga aggaaaacca cgtgatatta tccacagtca aaaaaatctt catgctgatc
     2161 ttcatagtca attaaaacta atcaataatc aattatctga aatatcaaag gcaaattata
     2221 cctttttgaa aggtgcagaa gaagaattag aaatagaaaa tcaaaagctt gaagtaattg
     2281 aagatcttgg agttactaat gatgcatttg cactagaagg atggatacca agatcaaaaa
     2341 ttgaagattt aaaaatagca tttgaaagtt atagcaaggg aactctcctt tatgaattag
     2401 aaactaaaga caatccacca actcttcttg taaatcctaa aagattcaaa gtttttgaat
     2461 cttttgtaag attctactct ttaccctctg gtaacgagtt tgaccctact ctaatttttg
     2521 gtttaatttt tcctatattt tacggtttaa tgataggtga tgtcggatat ggcttggtaa
     2581 tactcttggt ttctttatgg gtaattagaa gagtgcagca tagaaaacgt aatttgacca
     2641 ttatgccaaa atttttacga aatttcgcca aaacaatttt acgtccttcc cagatggtca
     2701 agctagcaaa agctatgatt ccaggttgta ttattgctat tatattggga ttttgttttg
     2761 acttatattt cggatttcat ctaaatgcat atctcttttc atttttgaat aattttgggt
     2821 taaacttgcc agctgatggt gcgttattga atcctattgg aacttttggt ttgcgtaaac
     2881 ttttgttaat cagtggatat attggactag gtatggtttc atttggtctt gtacttggaa
     2941 tcctcaacag tatgagagag cgtaatataa aacatataat tagcaaagtt ggatggctat
     3001 tatttggttg gggaattgca ctggttggtt tagcaatgat aaatcatgta aacatcaacc
     3061 cattgcagaa tattcaagga gcaggttatt ttgcattatt atttggaggt ataggactaa
     3121 tgttttatgg tgagggtatc agagcactta tggaattacc atccattatt agtcatatac
     3181 tttcttatac tagg
//
LOCUS       DATAFW010000080         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1799296, whole genome shotgun sequence.
ACCESSION   DATAFW010000080 DATAFW010000000
VERSION     DATAFW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799296"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VHJ57_01930"
     CDS             <1..527
                     /locus_tag="VHJ57_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487325.1"
                     /translation="IVSPGTLVTWFNGDADHDHKITLTNEVNPENIIFDSDDFAFNEA
                     SNPIVLNDTGTFNYYETDVNEEDTHFVMNGTIEVVYQQDLNANTPAASSSNSTSTLGN
                     GDTAGVLMVPTEDIDTYVQDLKSKGFAIDSTHNFDDIRAGDQQTLLLWTTSGMNLDEI
                     ISTFQEITPGLPYS"
     gene            742..993
                     /locus_tag="VHJ57_01935"
     CDS             742..993
                     /locus_tag="VHJ57_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487326.1"
                     /translation="MQSNQEYLSLFTNTRAHNLIIHYIIIVFLADRELSLVISYIGVG
                     FIADLSSSSITIQLVARITMASGVLVYLMMKKTRKMTFN"
     gene            complement(1219..2307)
                     /locus_tag="VHJ57_01940"
     CDS             complement(1219..2307)
                     /locus_tag="VHJ57_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487327.1"
                     /translation="MKSKTVKADQKLIQGKKDTGYVFPPALEHKIENIIDGLRQGFLK
                     LLHDISPENATIIAEYILAMKTEINLSDHYRQDNIIALCELSKYFDNKPFKSITNRDR
                     IIAFLDRFRKSESFDPLHKWIGTYNTYRIYLSRFFKWLYYPNIESDKRPKPEVIQNIP
                     ILKRKEKSVYKPTDLWTAEDDLLFLKYCPSKRMKCYHAVTRDSACRPHEILKLRIKEV
                     QFKTAINGQYAEVFVNGKTGTRHLPLIDSIPFVKDYLDHEHPQPTNPEAAFIAGTKKS
                     LTKKLQPASIHKIYENFKKDYFSKLLESANVPHKDKQKIKDLLKKPWNPYIRRHSALT
                     SLSSILKESDLRVLAGWISGSEMLLEKI"
     gene            2552..3079
                     /locus_tag="VHJ57_01945"
     CDS             2552..3079
                     /locus_tag="VHJ57_01945"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX2487328.1"
                     /translation="MSIGLIGTICGLVIVFLFLLVFNNIAYAQSSNIQYVSSSNSTLS
                     VIGNAETMVKPDKVTLTLSVETTNKTANAALIANSETMNNVLEALKSSGVKENETSTS
                     FFNISPNYNITQEEDFRPIQSRDIIGYTVTNSITVDSPNLLNISQWIDTAVQAGANVG
                     EQKVTSSVNITFLIN"
     gene            complement(3162..3284)
                     /locus_tag="VHJ57_01950"
     CDS             complement(3162..3284)
                     /locus_tag="VHJ57_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX2487329.1"
                     /translation="MIEVPSEADIETARSTYKNGILEIVLNKKAKPKGKQINVE"
BASE COUNT         1062 a          548 c          582 g         1103 t
ORIGIN      
        1 cgatagtcag tccaggtaca ttggttacat ggtttaatgg cgacgctgat catgatcata
       61 aaataactct tacgaatgaa gtaaatccag agaatataat atttgatagc gatgattttg
      121 catttaacga agcctcaaat ccaattgttt tgaatgacac tggcacattc aactattatg
      181 aaactgatgt aaatgaagaa gatactcact ttgtgatgaa tggaacaata gaagtcgtct
      241 accaacaaga ccttaatgcg aacacacccg cagcctcaag ttctaattct actagcactc
      301 tgggtaatgg ggatactgct ggagtattaa tggtaccgac tgaggatatt gacacgtatg
      361 ttcaggattt gaaaagtaag ggcttcgcta tagatagcac acataacttt gatgatatac
      421 gtgcaggtga tcagcaaaca ttgctattat ggactacttc tggaatgaat ttagacgaaa
      481 taatatccac gtttcaggaa atcacaccag gattgcctta tagttaataa cgctaaataa
      541 ttgaaatccg attcttttat ttcctttcca cttgcattca cttcaatcta gggataccaa
      601 gagcactgat gggtataagc gaaccaatcc ctgtatacta acctattacc acttggcgaa
      661 ctggaaaagt caatgaagaa aagaatatca tgagcacgaa aatgtcatca atattggtgg
      721 cggcaaaggc agcaacgcct attgcaatca aaccaagaat atctatcatt gtttacaaat
      781 actagagccc ataatcttat aatacattat ataattattg tttttctggc ggatagagaa
      841 ttatctctag ttattagtta tataggtgta gggttcattg cagatttatc aagcagtagc
      901 attacaattc agttagtagc aaggattaca atggcatcag gtgttttagt ttatttgatg
      961 atgaagaaga caagaaagat gactttcaac taaagactgt atattttaat accatactac
     1021 aaatagaact atacaacagg tttactaatt tagtattagt gtgtgcggaa tgtcaatgtg
     1081 caccggaaga atgtgctaaa gatattagca attttactca atgtaaaaac tgcacaaaac
     1141 gtatttgttg ctgcatagac cttcaccagg atttatcaaa taatactggc cattctgata
     1201 atcgcattgc atttcattct agatcttctc caatagcatt tctgaaccgg atatccaccc
     1261 agcaagaaca cgtagatcag attctttaag aatacttgat aagctagtta gtgcgctatg
     1321 tctcctaata tatggattcc agggtttttt taacaaatct tttattttct gtttgtcttt
     1381 atgtggaaca tttgcagatt ctagcagttt ggaaaaataa tcttttttaa aattttcata
     1441 gatcttgtga atagaagctg gttgtaactt ttttgtaaga ctttttttag ttccagctat
     1501 gaatgccgct tctgggttgg ttggctgtgg atgttcgtgg tccagataat ctttcacaaa
     1561 aggaatcgaa tcaattaatg gcaaatgacg tgttccagtc ttcccgttaa caaagacttc
     1621 cgcatattga ccatttatag cagtcttgaa ttgcacttct ttgattctta attttaatat
     1681 ctcatgtggt cgacaagctg aatccctagt tacggcatga tagcatttca ttcgctttga
     1741 aggacaatat tttaagaata gtaaatcgtc ctcagcagtc cataaatcag tgggcttgta
     1801 tactgatttt tcttttcttt ttagaatggg aatattttgg atgacttctg gttttggtct
     1861 tttatccgat tcaatatttg gataataaag ccacttgaaa aatcgtgaca gataaattct
     1921 gtatgtattg taagtcccaa tccatttatg cagggggtca aaactttcag attttctaaa
     1981 gcggtcaaga aacgctataa ttctatccct atttgtaatt gatttgaatg gtttattatc
     2041 aaaatacttt gaaagttcac ataaagcaat aatgttgtct tgtctgtagt gatctgacag
     2101 attgatttct gttttcattg ccagaatata ttcggctatt atggtcgcgt tttcggggga
     2161 tatatcatgt aataatttca gaaaaccctg ccgaagacca tctattatgt tttctatctt
     2221 gtgttccaaa gctggtggaa acacataccc ggtgtctttt ttcccttgta ttaatttttg
     2281 atctgctttt accgtctttg acttcactat tccactgtga aaagccctta aagtcacgca
     2341 taaaattaag ataagttgat tatataagtt agtattatat acgcacttat aaattaataa
     2401 aaaagtttga gtactatttt gtataattag agaggaagaa aatagaagat aaagtgaata
     2461 gaaaaagaaa accttttgac agctactgtg ttaactcaat ttcgtaataa acacaagaat
     2521 gttgataaat ctcgagcgat tataaagaca tatgagtatt ggacttattg gtaccatttg
     2581 tggcctagta attgtattct tatttttgtt agtttttaat aacattgcat acgcccagag
     2641 ttcaaatatt caatatgtaa gtagcagcaa cagtacgtta tctgttatag gaaacgctga
     2701 aacaatggta aaaccggata aagtaacact tacattatct gttgagacaa caaataaaac
     2761 agcgaatgca gccttaattg caaattcgga gaccatgaat aatgtgcttg aagctctaaa
     2821 atcgtcaggt gttaaggaaa acgagactag tacttcattt tttaatatct ctccaaatta
     2881 taacattaca caggaagagg attttcgacc tatacagagt agagatatca tcggctatac
     2941 tgtaactaat tccattacag tagattctcc caatctcttg aacatatcac aatggattga
     3001 tactgcagta caagcagggg ccaatgtggg agaacaaaaa gtaacctcta gcgtaaatat
     3061 tacatttctt atcaattaat tgcgtaatca agcctagatg gcatcgatat cttctttata
     3121 cataaaataa taaaagaaat aaagaagagg cttcctcttt attattctac gtttatttgt
     3181 ttgccttttg gttttgcttt tttgtttagt actatttcga gtatcccatt cttatatgtc
     3241 gatctagcag tttcaatatc ggcttctgat ggaacttcaa ttatcttcct gtatt
//
LOCUS       DATAFW010000081         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1272815, whole genome shotgun sequence.
ACCESSION   DATAFW010000081 DATAFW010000000
VERSION     DATAFW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272815"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VHJ57_01955"
     CDS             <1..402
                     /locus_tag="VHJ57_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487330.1"
                     /translation="DYLDMKKENSTKIIKIPNDGLWLKPGETYLGRTIEYTVTKNLVP
                     IIKTRSSIARLHINIINSAGFGDIGYSGHWTIPISVIKKTKVYPFVTICQIYYNDITG
                     EIDKEYNVNNKYQNSRDIMASKLYKDFDPNI"
     gene            839..2167
                     /locus_tag="VHJ57_01960"
     CDS             839..2167
                     /locus_tag="VHJ57_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487331.1"
                     /translation="MILKFHMEVDLLVNMENDAMLIEKQAEELKFRARKDSARYDPLF
                     MVGTEVEGCLLDDKGNSVNASQLIEELQNSKFFQNSGCMIDYEYGSGQFEFKTSPISI
                     TNLLDLNLLYEEFIIEHLEKTIQKVYKEKIVIPVFLGANPSPNILQDNIVTDKDRYKK
                     LFEWQSKFADIEFEGKTFKAAHIAAAIQGFHFHLQGRNPTYAVLMFNHILNLISSSII
                     LGASSKLFAGKVFSFHEPRIYLYDQSEQQNSGFPAITTYLKNIDNYIDYIKSRKPIIA
                     RDYFDLEKERHDDVRIRLNSDFYRVETRILSVQPTPKALMAMIEFFVGYIYRAIHEES
                     YGEKLLRSLPILREERQASVQSGFNATSHFPMVDTIKVQLDYARKGLSDLNIKPEFLN
                     ILDSRVKNRISPSEYVASLWDKKFNGSVTETIFEVISHIWERTKKNEPIM"
     gene            2273..>2571
                     /locus_tag="VHJ57_01965"
     CDS             2273..>2571
                     /locus_tag="VHJ57_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487332.1"
                     /translation="MNKIDYLIDSHMEDLISDLQILIRQPSVSARNNGVLECSLLVKN
                     LMEKAGIKTELLFPQQKKDIVTDNNFNIEPKIDRHNLSPPIIYGEVQSKRNPQGKT"
BASE COUNT         1008 a          347 c          376 g          840 t
ORIGIN      
        1 gattatttag atatgaaaaa agagaattca actaaaatta taaaaattcc aaatgatggt
       61 ctatggctta aacctggaga aacatatctt ggcagaacaa tcgagtatac agtaactaaa
      121 aatttggtac ctattatcaa aacacgttct tctatagcaa gacttcatat taatataatt
      181 aatagtgcag gatttggaga tataggttat tctggtcatt ggacaatccc tatttctgta
      241 ataaaaaaga caaaggttta tccttttgtt actatctgtc agatctatta taatgatatt
      301 actggagaaa ttgataagga atataatgtt aataacaagt atcaaaatag tagagatata
      361 atggcctcaa aactatataa agactttgac ccgaatattt aataagtaac tatagaacta
      421 aatagacttt actaaaaggt atacataact cggaatgtta aatttgaaat ttataatatc
      481 cttttatatt tgaaaattta aagatccccc tattaaacaa gaaccaaatt gttaattaaa
      541 ttctgtaagc caagattatc tcagctatac aggaaattaa ttaattcaat attactgttt
      601 gctgttttaa tgtaatagat atattcatta taattataaa atagatagat ctagaataga
      661 acttatatgt tctagacaat aaataaaata actattcttt aaattattac caagctttaa
      721 aaataatcaa ttagtaataa gcgaatttta attaaaataa ataaataata tatactatac
      781 atatttctat atatgaaatt acaaatttac aatactccaa aggcttgcat taatatacat
      841 gatccttaaa tttcatatgg aggtagatct cttagttaat atggaaaatg atgccatgct
      901 tatagaaaag caggcagaag aattaaagtt tagagcacga aaagatagtg ctaggtatga
      961 cccactattc atggtaggta cagaagtaga gggatgtttg ttagatgata aaggaaactc
     1021 tgtcaatgct tctcaactaa tcgaagaatt acaaaactct aaatttttcc aaaattctgg
     1081 ttgtatgata gattatgagt atggatcggg tcaattcgaa tttaaaactt ctccaatatc
     1141 gattacaaat ttgttagatc taaatttatt atatgaagaa tttataattg aacacttaga
     1201 aaaaacgatt caaaaagttt acaaggaaaa aatagttatt ccagtcttcc ttggtgcgaa
     1261 tccgtcacca aatattcttc aagataatat agtaacagat aaagatagat ataaaaaatt
     1321 atttgaatgg caaagtaaat ttgctgatat agaatttgaa gggaaaacgt ttaaagctgc
     1381 acatatagca gctgcaattc agggatttca ttttcattta caaggacgaa atcctacgta
     1441 tgcagtctta atgtttaacc atattttaaa tcttatttca tcttcaatca tacttggtgc
     1501 tagtagcaaa ctttttgctg gaaaagtatt ttcctttcat gaaccaagga tatatctata
     1561 tgatcaatct gaacaacaaa attcaggatt tcctgcaatt acaacatatc taaaaaatat
     1621 agataattat atagattata taaaatcaag aaaacccata attgccagag attactttga
     1681 ccttgaaaaa gaaaggcacg atgatgtacg catacgtttg aattctgatt tttatcgtgt
     1741 tgaaacaagg atattatcag tacagccaac accaaaagct ttgatggcta tgattgaatt
     1801 ttttgttggc tatatataca gagctatcca tgaagagtcc tacggtgaga aactgctaag
     1861 aagtctccca atattgagag aagaaagaca agcatcagta caatcaggtt ttaatgctac
     1921 atctcatttt ccaatggtag atacaattaa agttcaactt gattatgcca ggaaaggatt
     1981 atctgatcta aatattaagc ctgaattttt aaatatttta gatagccgag tgaaaaatag
     2041 aatctctcca agcgaatatg ttgcctcatt atgggacaag aaatttaatg gttctgttac
     2101 tgaaactata tttgaggtaa tttctcatat atgggaaaga acaaaaaaaa atgaacccat
     2161 aatgtagtat ggtaattgtt tataataaat tactattcat tatttctttt ttatttttac
     2221 tgaaatttta aaatttaact atatattatt aatattcata agaatattca gtttgaataa
     2281 aattgactat ctaatagatt cacatatgga agatctcata tcagatcttc aaatcttaat
     2341 aaggcaacca agtgtttctg ctaggaacaa cggggtcctt gaatgttctc ttttagtaaa
     2401 aaacctaatg gaaaaggctg gcataaagac ggaattactt tttcctcaac aaaaaaagga
     2461 tatagttacg gataataatt tcaatataga gcctaaaatt gatagacata atttatcacc
     2521 tcccataata tatggagaag tccaatcaaa aagaaatcct caaggtaaaa c
//
LOCUS       DATAFW010000082         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2152358, whole genome shotgun sequence.
ACCESSION   DATAFW010000082 DATAFW010000000
VERSION     DATAFW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2152358"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VHJ57_01970"
     CDS             <1..627
                     /locus_tag="VHJ57_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX2487333.1"
                     /translation="QIVKLLQNLGIDDEMPVVVYDDTFGALAARVAWTFQYVGHENTS
                     LLEMTYTNWKNLGLEIETRINKFPETTHSLKLNQSIYADATYVESIQNNPDKVLVDSR
                     ERLNFLSEHIPSAKNIPYTMLGNDNHILRSVDELKRFVENRGIPQESEAITYCGSVGT
                     LSGLAFYALKLAGIKNVKLYSKSFKDWKSLGKPKIEFKDANYWDLSAE"
     gene            complement(717..1106)
                     /gene="hsp14"
                     /locus_tag="VHJ57_01975"
     CDS             complement(717..1106)
                     /gene="hsp14"
                     /locus_tag="VHJ57_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal heat shock protein Hsp14"
                     /protein_id="HEX2487334.1"
                     /translation="MGIATYMAKEVMKEINNKSKEFYEFILPPVDMVEDSNDLLVIID
                     LPGFNKNDITLRINKNVLSIKATKPDNEYRGTVYLKHRPTKIDKKVILPFLVDEDEEI
                     TGTANYSDGIITLRIPGKNLGNRIPIS"
     gene            1193..2467
                     /gene="truD"
                     /locus_tag="VHJ57_01980"
     CDS             1193..2467
                     /gene="truD"
                     /locus_tag="VHJ57_01980"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX2487335.1"
                     /translation="MGNFVIKSVPEIDFHAGMECYCTNFAGINGTIKETNEEFQVSEI
                     IDYTFLKPPYFSSVQNNEHKFPLYLLEKHNIDSNHAVIEIKKQSGLKLRIMGIKDAKA
                     ETSQYASCEQTKNLPKCIITSKTKLTLLGFLRTPLSKSALIGNNFKIKINQPSDNNIH
                     PFSLELNKIANFYGLQRFGSERMVTHLVGKALLERNYNKTIEYLLSYTSKYDSKFSKE
                     IREKSRDPKNYQKLIKILPKGMDLERLILYTLVKGKDSIAVIRSIPINIRRLFVQAYQ
                     SYIFNKCLSNALLCNEDIQRCKDGDLCFEIEKPLVFGKIRRFREGIDSNNDVIPAIRL
                     VGYNYQPGKNRFDNITKRILSDENISPTAFYLKDLQELSLQMGFRQTSLCCSEFSHFG
                     MLDLSFKLPKGSYATTLLRELIKPIDPIQAGF"
     gene            2535..2897
                     /locus_tag="VHJ57_01985"
     CDS             2535..2897
                     /locus_tag="VHJ57_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487336.1"
                     /translation="MESNVSMVEGIINTLSEFETNVENINSSIEELKKRMLSHSNSEI
                     EQLREKIIKLANEEAQGILDDAKNESEKESLSIQEEGEKNYNLIKNNIEKNHDKAIDK
                     AINMILGNIDNEPNFDRT"
     gene            2979..4028
                     /locus_tag="VHJ57_01990"
     CDS             2979..4028
                     /locus_tag="VHJ57_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX2487337.1"
                     /translation="MGKANKPQRIFGTVRAFSERGTMLSKHDLESFSDSKTLDELVTR
                     LKNTSYVDSLSKIQQPITSEKIEIALREDLFNFHSSMAKIAKGDLVNAYYKKYILWNL
                     KIILKSKALGKTYDDIFPHINMQAEELIGRRDVIVKALIAKDLDETVSALQTSEFGDD
                     ISSAAQLYKENNNIQIFELYLDKIYYKGVISAFANIGKPADLQPLVSLDLDYYNVLST
                     LRAKYWELPEEHIKSLRIATTSRVNEDVLNKMIHADSVKQAIAEFSKTVYKDIVPNQG
                     NESDITIISQIESSFEELIHKKSMALYGRMFSYAPMTAAIKLKMTEIKNLVAIAFGIE
                     QKMDPKNIIDRLLLI"
     gene            complement(4099..5343)
                     /gene="ilvA"
                     /locus_tag="VHJ57_01995"
     CDS             complement(4099..5343)
                     /gene="ilvA"
                     /locus_tag="VHJ57_01995"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX2487338.1"
                     /translation="MNNELNLETKNLPTYDEIIKGRELLKKNFIGKTPLYKSNTFSNL
                     IGTNIFFKNEAFQKTGSFKVRGALYKINSLSEEEIKYGIIAASAGNHAQGVAYAATIR
                     KIPCTIVMPISASPAKIAATRSYNAQVILKGADYDEAWIAAKEIARNENKAIIHAFDD
                     PEVIAGQGSIGLELLEDLPDLDEIYLPVGGGGLAAGVAIAIKSKKRNIKIIGVESKSF
                     PAMKESIEKGSLQTKKGEFTIADGISVKSPGNLTFEIAKRYLDDIVLIDDDSIVKTMF
                     LLMERSKLVVEPAGAASLAYLLINGHKNKNKKIVSILSGGNVDMYLLGQVVAKGLLQM
                     NRLLKIFIQLPDKPGALKTVVDGISDLSINIVEVVHDRLSANISPGTAGVYLSLELEN
                     AKHCELLISLMKKKGIDFQIIT"
     gene            complement(5427..>5637)
                     /locus_tag="VHJ57_02000"
     CDS             complement(5427..>5637)
                     /locus_tag="VHJ57_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487339.1"
                     /translation="EEGVISYFEIETIKNKKTGKREPYLDTKKFNKEITEIVTSKLNE
                     IASSLKELRTNTNMKVDEDIKINTR"
BASE COUNT         2006 a          816 c          804 g         2011 t
ORIGIN      
        1 cagattgtca aattgttaca aaatcttggt atcgatgatg aaatgcctgt agtagtatat
       61 gatgatactt ttggagcttt ggcagcacga gttgcttgga catttcaata tgttggtcat
      121 gagaatactt cacttctgga aatgacctat acaaattgga aaaatcttgg tttggaaatt
      181 gaaactcgca tcaataaatt cccagaaaca actcattctc ttaaattaaa tcaatctatt
      241 tatgcagatg ctacatatgt tgaatcgata caaaataatc ctgataaggt attagttgat
      301 tctagagaac gacttaattt tctatctgaa catattccta gcgctaaaaa tattccatac
      361 actatgttag gtaatgataa ccatattttg cgatctgttg acgaattaaa acgttttgtt
      421 gaaaatagag gtattcctca agaatcagaa gcaattactt attgtggtag tgtaggaaca
      481 ttatctggtc ttgcatttta tgcattaaaa ttagcaggta tcaaaaatgt gaaattatat
      541 tctaaatcat ttaaagattg gaaatcatta ggaaaaccaa aaattgaatt taaagatgca
      601 aattattggg atttatcagc agaataataa ataaatagat aaaacaacta atcaaattat
      661 gaagaatttg taaaattata attttatatc aaaggattca gttctctatg tatttattag
      721 gaaattggta ttcgatttcc aagatttttg ccaggaattc tcaacgttat aattccatca
      781 gaataattag cagttccagt gatttcttca tcctcatcaa ctaaaaaagg caaaataact
      841 tttttatcta ttttggtagg acgatgcttt aaataaactg tacctcggta ttcgttatca
      901 ggcttagtgg ccttgataga gagcacattt ttatttattc taagagttat gtcattttta
      961 ttaaatcctg gtaaatctat aattacaagc aaatcattac tatcttctac catatctaca
     1021 ggaggtaaaa taaattcata aaactccttc gatttgttat taatttcttt cataacttct
     1081 ttagccatat atgttgcgat tcccataatt aataatatgc tatttgaata taaatccttt
     1141 atttattgtt gattgtataa ataattgttg tattgtttat atttaatttg atatgggtaa
     1201 ttttgtgata aaatctgtac ctgagataga tttccatgct ggtatggaat gttattgtac
     1261 aaattttgca ggtataaatg gaaccataaa ggaaactaat gaagaatttc aggtatctga
     1321 aataatagat tatacatttt taaaaccacc atatttttct tcagttcaaa ataacgaaca
     1381 taaatttcca ctttatttat tggaaaaaca taacattgat tctaatcacg cagtaattga
     1441 aattaaaaaa caatcaggtt taaaactaag aataatggga ataaaggatg caaaagcaga
     1501 aactagccaa tatgctagtt gtgaacaaac aaagaattta cctaaatgta taataacatc
     1561 taaaacaaaa ttgactttat taggtttcct acgtactcca ttatcaaaat ctgcacttat
     1621 tggaaataac tttaaaataa aaattaatca accttccgat aataatatac accctttttc
     1681 attagaatta aataaaattg ctaatttcta tggcttacaa agatttggaa gtgaaagaat
     1741 ggtaactcat ttagttggta aggcattact tgagagaaat tataataaaa ctattgaata
     1801 tcttctctct tatacttcca aatatgattc caagttctca aaggaaataa gagaaaaaag
     1861 tagagatcct aagaattatc aaaaattgat taaaatcttg cctaaaggaa tggatttaga
     1921 aagacttatt ttatatacac ttgtgaaagg taaagattct attgcagtta ttagatctat
     1981 accaataaat attagaagac tctttgttca agcttaccag tcttacattt ttaataaatg
     2041 tctcagtaat gctcttttgt gcaatgaaga tattcaaagg tgtaaagatg gagatttatg
     2101 ttttgagata gagaaacctc tagtttttgg taaaattaga aggtttagag aaggaataga
     2161 ttcaaataat gatgttattc cagcaattag attagtaggt tataattatc aaccaggaaa
     2221 aaatcgtttt gataatatta ctaaaagaat tttatcagat gaaaatattt cgccaactgc
     2281 tttttattta aaagatttgc aagaattaag cttacagatg ggattcagac agacttcgct
     2341 atgttgtagt gaatttagcc attttggtat gcttgatctg tcatttaaat taccaaaagg
     2401 atcttatgcc acaactttgt tacgtgaatt aataaaacca atagatccta ttcaagcagg
     2461 attttaaaag attgaaaata atttttacta attaaaagta aggtttaata tccttgaagt
     2521 gcaattttga tgaattggaa tctaatgttt ccatggtgga aggaataatc aatactcttt
     2581 cagaatttga aactaatgtt gagaatatta attctagtat tgaagaatta aagaaacgta
     2641 tgttgtctca ttctaatagc gaaatagaac aattgcgaga aaagattatt aaattagcaa
     2701 atgaagaagc acaaggaatt ttagacgatg ctaagaacga gtctgaaaag gaatctctct
     2761 caatccaaga agagggtgaa aaaaattata atctgataaa gaataatatt gaaaaaaatc
     2821 atgataaggc aatcgataag gcaattaata tgattctagg aaatatagat aatgaaccta
     2881 atttcgacag aacctagaca ttatagctat tgtttcttgt atcatcaaaa atatttaatt
     2941 aataaggtag tatcataaaa gaatgtggat gactaaaatt gggaaaagct aataaacctc
     3001 aaagaatttt tggaacagtt agagcttttt ctgaacgtgg tacaatgctc tctaaacatg
     3061 atttagaatc attttctgat tccaaaactt tagatgaatt agtaactcga ttaaaaaata
     3121 catcctatgt tgattctttg agtaaaattc aacaacctat aacatccgaa aaaattgaaa
     3181 tcgcactaag agaagatctt ttcaattttc attcttctat ggccaaaata gctaaaggag
     3241 atcttgtaaa tgcttattat aaaaaataca tcctatggaa tctcaaaata attttaaaaa
     3301 gtaaagcttt aggaaagacc tatgatgata tttttccgca tataaatatg caagcagaag
     3361 aactaatagg aagaagagat gttattgtta aagctctaat tgcaaaagat ctcgatgaaa
     3421 ctgtctctgc cttacaaacc tctgaattcg gtgatgatat ttcttcagct gctcaactct
     3481 acaaagaaaa taataatatc caaatttttg agctctatct tgataaaatt tactacaaag
     3541 gcgtgattag tgcttttgct aatataggga agcctgctga tcttcaacct ttagtttctc
     3601 ttgatttaga ctattataat gttttaagca ctcttagggc aaagtattgg gaacttccag
     3661 aagaacatat taaatcatta aggatagcaa caacctctcg agtgaatgaa gatgttctaa
     3721 ataaaatgat acatgcagat agtgtaaaac aagcaatagc agaattttcc aaaactgttt
     3781 ataaagatat agttcctaac caaggaaatg aaagtgatat cactataatc tcacaaattg
     3841 aatcgagttt tgaagaatta atacataaaa aatcaatggc attatatgga agaatgttta
     3901 gctatgcgcc tatgactgca gctattaaac taaaaatgac agaaattaaa aacctagtag
     3961 ctattgcttt tggaattgaa caaaaaatgg atcccaaaaa tattatagat cggctattat
     4021 taatttaata gatcttctga ttatttatat ttgataataa attatttaaa ttattattta
     4081 tttttatttt tttatttgtt atgtaattat ttgaaaatca ataccctttt tcttcatcaa
     4141 gctaattaaa agttcacaat gtttcgcgtt ttccaattct aaacttagat aaacacctgc
     4201 agttcctgga gatatattgg cactaagcct atcgtgaact acctctacaa tatttatact
     4261 taaatctgaa ataccatcta ctactgtctt taatgctcct ggtttatctg gtaattgaat
     4321 aaatattttt aataatctgt tcatctgtaa aagacctttt gctaccactt gtccaagcaa
     4381 atacatatct acattaccac ctgataagat tgatactatt ttcttatttt tatttttatg
     4441 tccattaatt agtaaatatg caagacttgc agcacctgca ggttctacta ctagtttaga
     4501 tcgttccatt aataagaaca tagttttaac aattgaatca tcatcaatca atacaatatc
     4561 atccaaatat ctttttgcta tctcaaaggt taaatttcct ggtgatttca cggatattcc
     4621 atctgcaatt gtaaattctc ctttttttgt ttgaagcgag cctttttcaa ttgattcttt
     4681 cattgctgga aaagatttag attctactcc aatgatcttg atattccttt ttttggattt
     4741 tatagcaatt gcaactcctg cagctaatcc accgccaccc accggaagat aaatttcatc
     4801 taaatctggt aggtcctcta ataattctag tcctattgaa ccttgtcctg ctattacctc
     4861 tgggtcatca aatgcatgaa taattgcctt attttcattt cgagctattt ctttagctgc
     4921 tatccatgcc tcgtcgtaat cagcaccttt gaggataact tgtgcattat aagatctcgt
     4981 agccgcaatc tttgctggag atgcacttat tggcattact attgtacatg gaatctttct
     5041 aatagttgct gcataagcta caccttgtgc atgattccct gcagaagcag ctattattcc
     5101 atattttatt tcctcttcag agagagagtt tattttgtaa agagctcctc ttaccttaaa
     5161 cgatccagtt ttttgaaaag cctcattttt aaagaaaata tttgtaccaa ttaaattaga
     5221 aaaagtattt gatttataaa gaggagtctt acctataaaa tttttcttta ataattctct
     5281 acctttaatt atttcatcat atgtaggtaa gttttttgtt tctaaattta attcattatt
     5341 cattattata ccttagatta catatatcaa ttgtaattat aatttactat ttctttatac
     5401 tatttgttat ctactattag taaatcttat ctagtattaa ttttgatatc ttcatcaact
     5461 ttcatattag tattagttct taattccttc aatgaggatg ctatttcatt taatttacta
     5521 gttacaattt cagttatctc tttgttaaat tttttagtat caagatatgg ctctcgtttt
     5581 ccggtttttt tattcttaat agtttcaatt tcaaaatatg atataactcc ttcctct
//
LOCUS       DATAFW010000083         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3385854, whole genome shotgun sequence.
ACCESSION   DATAFW010000083 DATAFW010000000
VERSION     DATAFW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3385854"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VHJ57_02005"
     CDS             <1..1062
                     /locus_tag="VHJ57_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2487340.1"
                     /translation="LGKWSANRFIGKKRPLIAVFSLTHYCNFYCPMCPFGDSDKINQF
                     KFAKKNDLSTSEWKSIFDKVSKYCIWAIIEGGEPTSRPDFMELVTYLHTLNMPITLIT
                     NCSLLDTIDLKELKKYIQFITCSIDSVYEKSYCKIRGVSRDTYDKVMKNLLLLNENNI
                     PHYFNSVITKYNTEEFINQSYFDRAKELGSNAVSFTFVEDRSDVDYSLLPDRKTMIAV
                     CESIIDYGKRHTHPKIMIPLEYFQQIIDHGRGLYDECGVWKSIFINGNGTVLVPCWKY
                     NSPENTYNLLEQSIDEIWESPKWDIAKTCHDCQVLGCIWYSSQPVTTFAKNYMRGLSN
                     IISQQKPDILNKENNMCDN"
     gene            1567..2355
                     /locus_tag="VHJ57_02010"
     CDS             1567..2355
                     /locus_tag="VHJ57_02010"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX2487341.1"
                     /translation="MYIQNEYVDRKVSLDLKNVSKQYLQPHTERIHTVLENINIEILE
                     GEIVTILGKSGCGKSTLLNLIAGFENTYGGKILLHGKPVNDISPERIMMFQESALFPW
                     LTAYQNIEIALKMAKIPKKQREEIVMHYLEIVGLSDYSHSYIHQLSGGMKQRVALARA
                     LSLNPKVLLMDEPFAALDIAIKKSLYHELLALHNKHRKTILFVTHNINEAVLLGDRVI
                     VMSPQLYGIKNEYVIDIPKEQRSDNNPIIKNIINQITSDLYENN"
BASE COUNT         1078 a          385 c          412 g         1040 t
ORIGIN      
        1 ttaggcaaat ggtcagctaa tcgttttata ggtaaaaaac gccctctaat tgctgtgttt
       61 agcttaaccc attattgcaa tttttattgt cctatgtgcc cttttggtga ttctgataaa
      121 attaatcaat ttaaatttgc aaagaaaaat gatctttcga cttcggaatg gaaaagtatt
      181 tttgacaaag tatcaaaata ctgtatctgg gctattatag aaggaggaga gcctactagt
      241 agacctgact tcatggaatt agttacctat ttacatactt taaatatgcc tataactttg
      301 attacaaatt gttcattatt agatactatc gatctaaaag aattaaaaaa atatattcaa
      361 ttcattacct gtagtataga ttctgtttat gaaaaatcct attgtaaaat acgaggagtt
      421 tcaagagata cttatgataa agtaatgaaa aatcttttac tcttaaatga aaacaatatt
      481 cctcattatt ttaactctgt aataacaaaa tataatactg aggaatttat taatcaaagc
      541 tactttgata gggcaaaaga attaggatct aatgcagtat cttttacatt tgtagaagat
      601 agatctgatg ttgactattc tttattacca gataggaaaa ctatgattgc tgtatgtgaa
      661 agtataattg attatggcaa aagacatact catcctaaaa taatgatacc actagaatat
      721 tttcaacaaa ttattgacca tggaagggga ctatatgatg aatgtggcgt ttggaaaagt
      781 atattcataa atggaaatgg aaccgtactt gttccttgtt ggaaatataa ttctccagaa
      841 aatacatata acctattgga acagagcata gatgaaatat gggaatctcc caaatgggat
      901 attgctaaaa catgtcatga ttgtcaggta cttggttgta tatggtattc ttctcaacct
      961 gttaccacct ttgccaaaaa ctatatgaga ggtttgtcaa atataatttc tcaacaaaaa
     1021 ccagatattc taaataaaga aaataatatg tgcgataact aattaaattt gaaatgggat
     1081 ggcccaataa atggcctatc ccattgtgat gattcggctg ggatttattc atactggtga
     1141 gcttatgaat aatgtcatag atttttaata tgggataaaa ttatttaaga attttttgtc
     1201 attctaaaat tcattagtaa atgaactttt caattcaaat ttaataattt agaggagcct
     1261 aaaaattatt catatatgag aatatctcat tagtttaaca gttatgtagt catactctta
     1321 ctgctatcca taggattgat agttaaacta gatataataa ttctcttcaa tatgctaaat
     1381 ttacaatttc taggatgttt tttccaataa gtgactagaa attttaatta taatttattg
     1441 tatattttta ttttcaaaaa cagttattgt atttataata ttatgtgtgc atattataca
     1501 tatactttta ctattaagca tatttaaaat ccattaaata ttaacactat tatagatata
     1561 tcataaatgt atattcaaaa tgaatatgtt gatcgaaaag tttctcttga tctaaaaaat
     1621 gtctctaaac aatacttaca acctcatact gagagaatac atactgtact tgaaaatatt
     1681 aatattgaaa ttttagaagg agaaattgtt acaatattag gaaaatccgg atgtggtaaa
     1741 tctactcttc ttaatttaat tgcgggattt gaaaatactt acggagggaa aattttactt
     1801 catggaaagc ctgtaaatga catcagtcct gaacgaatta tgatgtttca agaatcagct
     1861 ctttttccat ggcttacagc ttatcaaaat attgagattg cattaaaaat ggcgaaaatt
     1921 ccaaaaaaac aacgtgaaga gatagtaatg cattatcttg aaattgtagg tctgtctgat
     1981 tattctcatt catatattca tcagctatca ggaggaatga agcaaagagt agctcttgct
     2041 cgagccttat cattaaatcc taaagtttta cttatggatg aaccatttgc agctttagat
     2101 attgcaataa aaaaatcttt ataccacgaa cttttggctc tacataataa acatagaaaa
     2161 acaatacttt ttgttactca caatataaat gaggctgtat tattaggaga cagagtcatt
     2221 gtaatgtctc cacaattata tggtataaaa aatgaatacg ttattgatat tccaaaagaa
     2281 caaagaagcg ataataatcc cattataaag aatatcataa atcaaattac ttctgatctt
     2341 tatgagaata attaattaat ttttattaat aaataaagtc acattcttaa gtttagttat
     2401 ttttaattat ttaaattaaa gattaactta agaagatatt gatataataa tataaatctt
     2461 gcaattataa taaagtttag ttcatatctg cttgttccca tttatgagaa gggttcactt
     2521 ttgatataat gctatagtaa cttttcaaag tataatacca aattaaaatt gtctgtatct
     2581 atcgagatat aaatataaaa tattggtcaa tttctgaaag accatgaaca cctaaatttt
     2641 gttattttct cggattaact agtatctcaa tagtctatag attattgcta atcatgattg
     2701 aaatttattt ttacactaca aaaaaagata tttatattat atcgtcagaa ttacctaact
     2761 taataaacca aaagaaatga atactcaact tttagataat aaagtaatac gttatacaac
     2821 aggtctatgt acgtttctgg ccggtgtata tatatcgcac ttattccccc tgttttttac
     2881 tcttctgaca atagatgtta aagtgttctt tagtt
//
LOCUS       DATAFW010000084         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_39213, whole genome shotgun sequence.
ACCESSION   DATAFW010000084 DATAFW010000000
VERSION     DATAFW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39213"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VHJ57_02015"
     CDS             <1..754
                     /locus_tag="VHJ57_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEX2487342.1"
                     /translation="IEGNFLQVYSSLRNLNPSPYMYLFNIDKRCIIGSSPEMLLRVTG
                     KVIETFPIAGTRPIGKNEQETIILGENLLKDEKELAEHTMLVDLARNDVGRVSDYGTV
                     KVKSLMAIKRFSHVQHIVSHIVGDLSDNFDVFDALKALFPAGTVSGAPKVRAMEIINE
                     LEKERREPYAGALGYFSFNGCCDFAITIRSIFIHGKQGYVQSGAGIVMDSIPINEWEE
                     TERKAEAMISVLKNKKNRKLERKSFTNSTRRV"
     gene            complement(808..2151)
                     /gene="thrC"
                     /locus_tag="VHJ57_02020"
     CDS             complement(808..2151)
                     /gene="thrC"
                     /locus_tag="VHJ57_02020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2487343.1"
                     /translation="MNRALKKCINPSCGLTYEIRDNNYQCSCGSFLDVLYPERPPKEL
                     IEIFYQRRNPNGNIYNESGVWRFRELLNFPEIDTENISECSRVLVSLDGAEGRLSKPY
                     NMSKVADFVDLPHENLFLQPEGYNPSGSFKDNGMSSAVTHAKMLNIHTLICASTGNTS
                     ASAAMYASNENMKCDVFIPKGEIAPGKLGQAFQFGAQVIQVDGNFDDALQTSLEYSKT
                     SNGYTVNSINPFRIEGQKTIIYRALEHLEWNPPDWIIYPGGALGNTSSCGKALIELYE
                     WGWIRKIPRIAVVNAQGANTLYELVNGKFENTNLHWNGGNPDINQIQRYYHSKKEKNM
                     LPKTRATAIQIGKPSNIIKGLRALWYTNGLVIQVNDDEMMDGMSIVGLNGFDCELASG
                     AVPAGIKKLREEDIIKKDDIVIGILTGRQKDASISMEYHMDTKNRFSNPPKNISQ"
     gene            complement(2334..2597)
                     /locus_tag="VHJ57_02025"
     CDS             complement(2334..2597)
                     /locus_tag="VHJ57_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487344.1"
                     /translation="MEEKKIKVNNREYNISLNDQTQLNAMKLKRLYQQSYSDMDSFDE
                     VSTEISSTINNLLKRSVSPEVLEEDMDGAIQQILKMYEKKGKK"
     gene            2918..>3330
                     /locus_tag="VHJ57_02030"
     CDS             2918..>3330
                     /locus_tag="VHJ57_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2487345.1"
                     /translation="MKVNSQSEISYCNKLSYMLNEIITKENARGLLLSGGLDSSIIAS
                     FQKPEYTFTVTLDGSNASDSHYARMIANQYSSNHIAIKIKTKELLTIEEKIIELFRTF
                     DPIFIRNSSVIFAAVQKANELGVSTLLTGDGGDEL"
BASE COUNT         1095 a          552 c          506 g         1177 t
ORIGIN      
        1 tattgaagga aacttccttc aagtttattc atcattacga aacctaaatc cttctcctta
       61 tatgtatctc tttaatattg acaaaaggtg tataatagga tctagtccag aaatgcttct
      121 tcgtgttaca ggaaaagtaa ttgagacatt tcctatagct ggaacgaggc ctatcggtaa
      181 aaacgaacaa gaaactatca ttttaggaga aaatttatta aaagatgaaa aggaattagc
      241 tgaacataca atgttggtcg accttgcaag gaatgatgtc ggacgtgttt ctgattatgg
      301 aacagtaaag gtcaaatcgc tgatggcaat taaacgtttt agccatgtgc aacacattgt
      361 atcacatatc gtaggagact tgtcagataa cttcgatgtt tttgatgcct taaaagcact
      421 atttcctgct ggaacggttt caggagcgcc caaagttagg gcaatggaaa ttataaatga
      481 attggaaaaa gaaagacgag aaccttatgc tggggctcta ggttacttct cattcaacgg
      541 atgttgtgat tttgctataa cgatacgatc aatttttata catgggaagc aaggatatgt
      601 tcaatctggt gctggaatag taatggattc cattcctata aatgagtggg aagaaacaga
      661 aaggaaagca gaggctatga tatcagtttt aaaaaataaa aaaaatcgta agcttgaacg
      721 aaaatctttt acaaatagta caagaagggt ttaaataaaa aattattgct tatcatttat
      781 ttacttcata atataattaa tgatggatta ttgagaaata ttcttcggtg ggtttgaaaa
      841 tctgttttta gtatccatat gatattccat agatattgat gcatcttttt gtctacccgt
      901 caaaatccca ataacaatgt catccttttt aataatatct tcttctctca atttctttat
      961 tcctgcagga actgctccgg aagcaagttc acagtcgaat ccgttgagtc ctacgattga
     1021 cattccatcc atcatttcgt catcattcac ctgaattact aatccattag tataccatag
     1081 agctcttaac cctttaatga tatttgaagg cttacctatt tgaatagctg tggctctagt
     1141 tttaggaagc atattctttt cctttttaga atgataatat ctttgaattt gattaatatc
     1201 tggattacct ccattccagt gtagatttgt attttcaaac tttccattaa caagttcata
     1261 aagagtattt gcaccttgtg catttacaac tgcaattctg gggatttttc gtatccaccc
     1321 ccactcatat aattctatca atgcttttcc acaactggat gtatttccta gtgcaccacc
     1381 aggataaatg atccaatcag gaggattcca ttctaaatgc tctaaagctc tgtaaattat
     1441 agttttctgg ccttctattc taaacggatt gatagagttg acagtataac catttgaagt
     1501 tttagaatat tctaaagagg tttgtagtgc atcatcaaaa tttccatcta cttgaataac
     1561 ttgagccccg aattgaaagg cttgtcctaa tttgcctgga gctatttctc ctttaggaat
     1621 aaaaacatca catttcatgt tttcattgga tgcatacatt gccgctgatg ccgatgtgtt
     1681 tccagtggat gcgcaaatta gtgtatgtat attgagcatc tttgcatgag ttactgcgct
     1741 tgacatacca ttgtctttga aagatccact cggattgtat ccctcgggtt gcagaaaaag
     1801 attttcatgt ggaagatcca caaaatctgc aacttttgac atattatatg gtttagacaa
     1861 tcttccttct gcaccatcga gagaaactaa tacccgtgaa cattcactga tattctcagt
     1921 atctatttca ggaaaattta ataattctct aaacctccat acaccacttt cattataaat
     1981 atttccatta ggatttctcc tttgataaaa tatttcgatt aattccttag gcggtcgctc
     2041 tggataaaga acatctagaa atgaaccaca agaacattga taattattat ctcttatttc
     2101 ataagtcaaa ccacaagacg ggttgataca cttttttaac gcacgattca ttcgaatgaa
     2161 tcattattat tatcttattt atattattgg tgtatgtttt ttactccttc taactttcaa
     2221 ttacttttca ttcaattatt tagttattaa aatgaggagg ttatatttac tcaattgtat
     2281 cataactacg tattataatg ttcaaacata attattatgt ttttaaagga ggtttacttt
     2341 tttccctttt tttcatacat tttgagaatt tgttgaatgg ctccatccat atcctcctca
     2401 agaacctcag gagaaacaga tcgctttaag aggttattaa tggtactcga tatttcagta
     2461 ctgacctcat caaaactgtc catatcacta tagctttgct gatataatcg tttaagtttc
     2521 atagcattta gttgagtttg atcattaagt gaaatattat actctctatt atttactttt
     2581 atcttttttt cttccataag atcataccct atattaagga tttaaaaaac tttctatgta
     2641 aaatttatct ttttagttca taattcataa taataacaat aataatttga taaaaatgga
     2701 taacaattat ttaaaaaaaa ccatccatta aaattttcaa ggttttatgt attcttcaat
     2761 ttaaaatata agtaacatat ttttcctcaa gtaataattt agacctataa atttatactg
     2821 atttaaggat tattcttaat tatccaacaa ttacgaatta gtcaaatgtt ttaagaacat
     2881 tataacattt atcttccttt gacatctaga tttataaatg aaagtaaatt cacagtctga
     2941 aatttcatat tgtaataaac tttcttatat gttaaatgaa attataacaa aagagaatgc
     3001 tcgcggcctc ctactttcag gaggtttgga tagtagcata atagctagtt ttcaaaaacc
     3061 tgaatataca tttacggtta ctctcgatgg ttccaatgca tcagactctc actatgccag
     3121 aatgattgca aatcaatata gctcaaatca tatcgcaata aaaataaaaa ctaaagaatt
     3181 attaactata gaagaaaaaa taatagaatt atttcgaaca tttgatccca tttttattcg
     3241 taactcaagt gttatttttg ctgcagtaca aaaagctaat gaattaggag tgtcgacctt
     3301 attgaccgga gatggagggg atgaattatt
//
LOCUS       DATAFW010000085         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_569148, whole genome shotgun sequence.
ACCESSION   DATAFW010000085 DATAFW010000000
VERSION     DATAFW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569148"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VHJ57_02035"
     CDS             <1..1263
                     /locus_tag="VHJ57_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487346.1"
                     /translation="LDGLVGVSQNGNKTVNRKNIEQLISGLDNRIFLMNNTHEDGLEM
                     LQTRWALSYLRGPLVKDQIKQLMAHMKKSKDFIETKTKSEDLDEPIVSNNDPNNDHTN
                     INSKSKNEIKEQPILSPEITQYFIPLSFLGTKKPNEFFYKPVILGAVSLNFIDSKLAV
                     DLIKDLTFITPITDGSIPVDWANSQQIEIDIKNLQKNPENANFMDLPPEASKPANYES
                     WKKDFIDWLYRNQKLNLFKVKNLKEISKVEESERDFKIRIDILIREDRDNMTQKLRQK
                     YASKMRSLQEKIQRTENILRLQQEQAQQQKIQTAFSLGSTLIGAFMGRKIARYSARRA
                     SDVMRGVTRTAKEKNDVNKALQTLQSYQRQFLDLETEFKAEVVEIENKIRRNLNDLET
                     ITLKLDKKDISIKLLSLTWVPHPTIPLN"
BASE COUNT          736 a          242 c          244 g          508 t
ORIGIN      
        1 ttagatggac ttgttggagt atctcaaaat ggtaataaga cagttaatag aaaaaatata
       61 gaacaattaa tttcaggttt agataacagg atctttttaa tgaataatac tcatgaagac
      121 ggtttagaaa tgttacaaac cagatgggca ctctcatatt tacgaggacc acttgtaaaa
      181 gatcaaataa aacaattaat ggctcatatg aaaaaatcaa aagactttat tgaaactaaa
      241 accaaatcag aagatttaga cgaacctatt gtttctaata atgatcctaa taatgaccat
      301 accaatatta acagtaaaag caaaaacgaa ataaaagaac aacctatact atctcctgaa
      361 ataactcagt attttattcc tttatctttc ctaggaacga aaaaaccaaa cgaatttttt
      421 tataaacctg tgatacttgg agcagtttcc ctaaatttta ttgattcaaa attagctgta
      481 gatttaataa aggatctaac ttttattacg ccaataactg acggatcaat tcctgttgat
      541 tgggcgaatt ctcagcaaat agaaattgat ataaaaaacc tacagaaaaa tcctgagaat
      601 gcaaatttta tggatttacc acccgaagca tcgaaacctg caaattacga gtcatggaag
      661 aaagatttta ttgattggct atatagaaat caaaaactaa atttatttaa agtaaagaat
      721 ttaaaagaaa tttcaaaggt tgaagaatca gaacgtgatt ttaaaattag aattgatata
      781 ttaattcgag aggatagaga taacatgact caaaaattac gccaaaaata tgcgtctaaa
      841 atgaggtcac ttcaagagaa aattcaaaga actgaaaaca ttttaagatt acaacaagaa
      901 caggcacaac aacaaaaaat ccaaactgct ttttccttag gaagtacatt aatcggagca
      961 ttcatgggta gaaagatagc tagatattct gcacgaagag cgtctgatgt tatgagagga
     1021 gtaacaagaa cagcaaaaga aaaaaatgat gtgaataaag ctttacaaac cttgcaatca
     1081 tatcaacgac aatttttaga cctagaaact gaattcaaag cagaagttgt agaaattgag
     1141 aataaaataa gaaggaattt aaatgattta gaaacaataa cccttaagct cgataaaaaa
     1201 gatatttcca tcaaattatt atctttaacg tgggtaccac atccaacaat accacttaat
     1261 tagaaaagaa tttaaaaaat gataacgtat tttactccat aaaaaaatta aatatttcaa
     1321 tgaaatcata caaaaagcta taagtatagt gaataactga tacccaattt tatattgtac
     1381 aaggtaaaat ctatttcaaa agatgaaaga tcgttcatct tcatatactt cagttacata
     1441 tgttacaaat taaaagaact tgtaaaaaat tttcattcac tattgttttc atttattatt
     1501 aaaaataaaa ttttataata gaagtaaatt aggctaataa caaatttgtt attcaaacct
     1561 ctgagcccga agtaaatggt tcagtaatcc ttataaataa attcaatgta gaacctgata
     1621 aggttggaca atttttaata gattgggaaa aggacgctac taattttaaa caacaactat
     1681 catttcaatg tctttataat attagaagat ataaccatgt taactaaagc
//
LOCUS       DATAFW010000086         7616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_569196, whole genome shotgun sequence.
ACCESSION   DATAFW010000086 DATAFW010000000
VERSION     DATAFW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569196"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /gene="larC"
                     /locus_tag="VHJ57_02040"
     CDS             complement(<1..417)
                     /gene="larC"
                     /locus_tag="VHJ57_02040"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEX2487347.1"
                     /translation="MSKIAIIDAQIAGLSGDMMLSALIDLGANKKKVIDSIYRCEDII
                     QGSKIKDVRFEKVNSNGFTATRFYFEYQDKIHERKGIEIIKNISKFTDYLDLKPRSKS
                     FIMDSIKTLIEAEAKIHGESLDQVYLHEASSIDTLAD"
     gene            572..1489
                     /locus_tag="VHJ57_02045"
     CDS             572..1489
                     /locus_tag="VHJ57_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX2487348.1"
                     /translation="MTITVVGSGKVGASVALNCALRELDDIILLDVIEGLPQGEAMDI
                     NHQLSERGIDSKIKGSNTYEDMKNSDFIVLVAGMGRKTGMTRMDLLKINASIVKDVAT
                     KILTHAKNATLVVVTNPLDPMTYLALKTTKINKNKVLGMGGMLDLSRFKSFIHDASGC
                     SRASIQAMVISEHGENMLPLIRFSSIGGLPISEFLSKKQCQEIFEKTKKVAAEVIALK
                     GATVYAPGNAVATMIESMKKNKKQVIPVSAYLDGEYGISDLCLGVPVVLGSNGIEKII
                     ELELNEFERKIFDLGVTSVREAINLINQK"
     gene            1494..2297
                     /gene="larB"
                     /locus_tag="VHJ57_02050"
     CDS             1494..2297
                     /gene="larB"
                     /locus_tag="VHJ57_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX2487349.1"
                     /translation="MEFEELLTQYNNGNISINELKKRITSSYIYNIGKNIANLDINRN
                     YRKGIPEVIYAANKDYPDIEKIILKVLKINSNIVVSKVKKEHKRKLINFVKKKNLYIE
                     IGRSSSTIFLSKISKFKYQHYKIGIISGGTSDIGIAEESRLMAKAMNCDSIISYDIGI
                     AGIHRIFPTLEKMISEDVQAVVVVAGMEGALASLVASCVNVPVIGVPTSVGYGFGSNG
                     IAALSSMLQSCALGLTVVNIDNGIGAGSFAALIANRMSTIKNPTVDKNE"
     gene            2363..3019
                     /locus_tag="VHJ57_02055"
     CDS             2363..3019
                     /locus_tag="VHJ57_02055"
                     /inference="COORDINATES: protein motif:HMM:NF006342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX2487350.1"
                     /translation="MNYINTLKRIRERKTNYHKRSSLLLSKKKFITIKVTNQNILTQI
                     IEPLITGDKVITSAHSTNLQKIGWKGSLNSLPACYLTGLLLGKKAIFKGINNAILYTG
                     KDTFTERISACLKGIIDGGIEIPSSQDSFPSTERISGEHISAYASDLKSDAGTYNKRF
                     STIIKNGLNPENYKSHFEECKEKIMNIDVQNFIRNENNNVHKSNKHDKINQTEGRLNQ
                     "
     gene            3016..3636
                     /locus_tag="VHJ57_02060"
     CDS             3016..3636
                     /locus_tag="VHJ57_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX2487351.1"
                     /translation="MSHQFRREEKQVEWKPRTTLGMLVAGGKISSMEQVFENGMRIQE
                     SEIVKQLLPDMKSQVVHVGIVQKQTDAGEMTRFDALVAVGNEGGWFGIGKGKAAQMRN
                     AIDKATTASYLNVIPIKLGCGSWECRCNQLHSIPFQVSGKGGSVTLEILPGPRGLGLV
                     AGEDIKKLLKLAGLKDAWTKSYGSTNTMASTTKAIFNALRSTYTAG"
     gene            3646..4104
                     /locus_tag="VHJ57_02065"
     CDS             3646..4104
                     /locus_tag="VHJ57_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX2487352.1"
                     /translation="MVYLVVRIRGIVNVPSWAHKTLENLNLRKKFNATLVSENNQTLG
                     MLRKVKDIVAWKSVDESIIKELLEKRGRKKGLHSVKDSIYPSNNKTIADLATVIHKNN
                     ISLSELVDLKPWFSLSPPKGGFKRKTKTQYTQKGTLGENNELINLVRRMI"
     gene            4190..4627
                     /locus_tag="VHJ57_02070"
     CDS             4190..4627
                     /locus_tag="VHJ57_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HEX2487353.1"
                     /translation="MATRLRKSRRQRGTRYCGWGQIGQHRQSGSRGGVGQAGKHKHFW
                     IRTVIEEPDHFGHPQSKSHHKIKVKKWANLRDLDLLYTEHIKNKSNERETLDLTTLGI
                     NKLLGAGRVTTKMTVKVEQISRRAVEKIKAIGGNVIFNEHSSE"
     gene            4608..6041
                     /gene="secY"
                     /locus_tag="VHJ57_02075"
     CDS             4608..6041
                     /gene="secY"
                     /locus_tag="VHJ57_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX2487354.1"
                     /translation="MNTVQNEGFIRKSVKTISRYIPQVEKPKKKISLTEKFVWCGIAV
                     FAYLVMGQVPLYGVTDEPQFDFLAFARVIFAAQQGTILELGIGPIVTAGLLMQLLKGS
                     DILKLNFKNPDDRALFTSATKIVTIIVIIAEGGLYGASVYGPLVASPDFVPILIAQLI
                     FTSLIVMLLDELIQKGWGLGSGISLFIMAGVAQTILWSVFSPVAGPDGPVGIIPFIID
                     SAATGDLSDTFFRSGQLPSIFTFILTGGVLALLVYTQGIHIDIPIVSTKYRGFTAVYP
                     IKLLYVSNIPVILATALVANAIFMGQMLWANYNPQNDNPFFNWIAQFDPGQPQTPTGG
                     LLYYITTPRGLDIVVQDPIRAVVNIIFVTAIVTVFGRLWVELGGLSAKAAAKNLLDAD
                     VQIPGFRRSQNSVQNVLDKYIPSVTIIGGIAIGLLASVSDVLGLFGGGIGLLLMVDIL
                     VNYYNLLIREKVDTTMPKLAALIGRKD"
     gene            6025..6609
                     /locus_tag="VHJ57_02080"
     CDS             6025..6609
                     /locus_tag="VHJ57_02080"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF003122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX2487355.1"
                     /translation="MVERTKRVVVVGIPGVGKTTIVSGVKSSLEEEGIKINLAEFGKI
                     MLEEATKLKIKNRDEIRRLSIEDQKKLQNLAANKISKIESDVTIIDTHLFITTKEGYY
                     PGLPLELLNHLKPTNLILLIANPEEIYHRRQNDSSRERDIISMDSIKNEIDVSKMLIS
                     CCSIISGAPFKILANHEGAVTDCTQKMVSLILDT"
     gene            6626..7225
                     /locus_tag="VHJ57_02085"
     CDS             6626..7225
                     /locus_tag="VHJ57_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HEX2487356.1"
                     /translation="MYLGDLILQIPGLTPTYTKHPIFPDLFSAMIVAAIISIGLNFAF
                     AILRKKTTDIDRLSKVMKETNEWRKEYTDAIRKQDKVRINELKKKQAYVNKMSLEMQQ
                     QQMRPMMLYMIPSFMLWILVFPSIFGQTVAISPLQIPYIMCTEENVKLDAAIDENGQP
                     KGVCNIPGEVFLWGWFLITSLGTSGLVAKLTKTSLPSMT"
     gene            7227..>7616
                     /locus_tag="VHJ57_02090"
     CDS             7227..>7616
                     /locus_tag="VHJ57_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487357.1"
                     /translation="MVTSSYKNKILNGIVISGWPAVGKTTIATELAKEFGYKHYNGGD
                     VLKILAAEKGYLVSGNDWWDTEEAKNFMEERESNPYFDIEVDRKLVEIVRMGKVVVTS
                     YTLPWLVPESIKIWLKGSRSNRIKRMAK"
BASE COUNT         2737 a         1088 c         1433 g         2358 t
ORIGIN      
        1 atcagctaaa gtatcaatac tggaagcttc atggagatat acttgatcta atgattctcc
       61 gtggattttt gcctctgcct ctatcaaagt ctttattgaa tccataataa aagacttaga
      121 acgaggtttt aaatccaaat aatctgtaaa tttggatata tttttaatta tttcaattcc
      181 ttttctttca tgaattttgt cttgatattc aaaatagaat ctagttgcag taaatccatt
      241 agaattaacc ttctcaaatc tgacatcttt tattttgctt ccttgaataa tgtcttcaca
      301 ccggtatatt gaatcaatta cctttttttt atttgctcct agatctatta atgcagataa
      361 catcatatcg cctgataaac cagcaatctg agcatcaata atagcaattt tcgacaactt
      421 aatacattat aataattatt aaacataaat taaaattact tattttatca agatgcttat
      481 caatacaata ttttctagtt actgctcgcc ctccaaaata tactttgaaa ttttcagtaa
      541 actataattt atccagaaat aattccactt tatgacaatt acagtagtcg gatctggaaa
      601 agtaggtgca tctgtggcct taaattgcgc attaagagaa ctagatgata taatactttt
      661 ggatgtgata gagggtcttc cacaaggtga agcaatggat ataaatcacc aattatctga
      721 acgcggaata gattctaaaa taaaaggctc aaatacttat gaggacatga agaattctga
      781 ttttattgtt ctcgtagctg gtatgggaag aaaaactgga atgactagga tggacttgtt
      841 gaagataaac gctagtattg taaaagatgt tgccactaaa attttaacac atgcaaagaa
      901 tgcaacctta gttgttgtta ctaatccact cgatcctatg acttatttgg cgttaaaaac
      961 tacaaaaatc aataaaaaca aggtattggg aatgggaggt atgttagatt tatctagatt
     1021 taaaagtttt attcatgatg ccagcggttg ctcaagggct tcgatccaag caatggtgat
     1081 aagcgaacat ggtgaaaata tgttaccttt aatacgattt tcttcaattg gtggacttcc
     1141 tataagtgaa tttttatcta aaaaacagtg tcaagaaatt tttgaaaaaa caaaaaaagt
     1201 tgctgcagaa gttatcgcat taaaaggtgc aacagtctat gcccccggaa atgcagtcgc
     1261 aaccatgatt gaaagtatga aaaaaaacaa aaaacaagta attcctgttt ctgcctatct
     1321 ggatggagaa tatgggattt ctgatttgtg tctaggtgtt ccagtcgtat tgggatccaa
     1381 tggtatagaa aagataatcg aactagaatt aaacgaattt gaaagaaaaa tttttgatct
     1441 aggagttaca agtgtaagag aagcaattaa tttgataaat caaaaataac ataatggaat
     1501 ttgaagaatt attaactcaa tacaataatg gaaatatatc aattaatgag ctaaaaaaaa
     1561 gaattacctc ttcctatatt tacaatatag gaaaaaatat tgcaaattta gatattaata
     1621 gaaattatcg aaagggaatt ccagaagtga tatatgcagc aaataaagat tatcctgata
     1681 ttgaaaaaat aattttaaaa gtattaaaaa ttaacagtaa cattgttgta agtaaggtca
     1741 aaaaggaaca taaaagaaaa ttaataaact ttgtaaaaaa aaagaatttg tatattgaaa
     1801 ttggtagaag tagctctact attttcttat caaaaatttc aaaatttaaa taccaacatt
     1861 ataaaatagg aattatttca ggaggtactt ctgatatcgg tatagcagaa gaatcaagat
     1921 taatggctaa agcaatgaat tgtgactcaa ttatttcata tgacatcggt atagcaggaa
     1981 ttcatcgaat atttccaact ctagaaaaga tgatttcaga agatgttcaa gccgtagtag
     2041 tagttgcagg tatggaaggt gccttagcct ccttggtcgc ctcatgcgta aatgtacctg
     2101 ttattggagt tcctacctcg gttggttatg gatttggttc taatggtatt gctgctctat
     2161 cttcaatgct acagagctgt gcacttggat taactgtagt aaatatagat aatgggatag
     2221 gagcaggttc attcgctgca ttgattgcta atagaatgag cactataaaa aatcctacag
     2281 tggataaaaa tgagtaatag aacgtttata agtgcttgta aaattctgca tttattatca
     2341 aaaaattgga gaaaaaacaa taatgaatta tataaacact ttaaagagaa ttagagaaag
     2401 aaaaactaat tatcataaac gctcatcgtt attattaagt aaaaagaagt ttataaccat
     2461 aaaagttaca aatcaaaata ttttaacaca aataatagaa cctttgatca ctggtgataa
     2521 ggtaattaca tcagctcata gtactaattt acaaaaaatt ggttggaaag gttctttaaa
     2581 ttctcttcct gcctgttact taacaggatt attactagga aaaaaagcca tttttaaggg
     2641 aattaataat gctatacttt atacaggtaa agatactttt acggaaagga tatcggcctg
     2701 tttaaaaggg ataatagatg gcgggatcga gataccatcg tctcaagact cttttccaag
     2761 cacagaacga atttctggtg aacatatatc tgcttatgca tcagatttaa agtcggatgc
     2821 aggaacttat aataaaagat tttctactat tataaaaaat ggtttaaatc ctgaaaatta
     2881 taaaagtcat tttgaggagt gtaaagaaaa aataatgaat atagatgttc agaattttat
     2941 aagaaatgaa aataataatg tgcataagtc taataaacac gataagataa atcaaactga
     3001 agggagacta aatcaatgag tcaccagttt cgtagagaag aaaaacaagt agaatggaaa
     3061 cctcgaacaa cattaggtat gcttgttgca ggtggcaaga tatcttctat ggaacaagtt
     3121 ttcgaaaacg gaatgcgtat ccaagaatct gaaattgtaa aacaactact tcctgatatg
     3181 aaaagccaag tagtacatgt cggaattgtt caaaaacaaa ctgacgctgg tgaaatgacc
     3241 agatttgatg cattggttgc agtaggaaat gaaggcgggt ggtttggaat aggtaaagga
     3301 aaggcagcac aaatgcgaaa tgcgattgac aaagctacta ctgcttctta tctaaatgtt
     3361 attccaataa aacttggatg tggcagttgg gaatgtagat gtaatcaatt acattcgatt
     3421 ccatttcagg tatctggaaa gggtggaagt gtaacgcttg agatattacc cggccctaga
     3481 ggattagggt tggtagcggg agaagatata aaaaaactat taaaattggc tggacttaaa
     3541 gatgcatgga ctaaatccta tggctcaaca aatactatgg cttcaaccac aaaagctata
     3601 ttcaatgctt taagaagtac atatacagca ggttaatgat gtaatatggt gtatttagtt
     3661 gttagaattc gtggaatagt taatgttcca agctgggcac ataaaacttt agaaaattta
     3721 aacctgagaa aaaagtttaa tgcaacccta gtttcagaaa ataatcaaac cttaggaatg
     3781 ttacgtaaag ttaaagacat tgttgcttgg aaatctgttg atgaatcaat tattaaagaa
     3841 ctcttggaaa aaagagggag aaaaaaagga ttacattcgg taaaggattc tatttatccc
     3901 tcaaataaca aaactatcgc tgatttagcg actgtgattc ataaaaataa tattagttta
     3961 tctgaattag tagatctgaa accttggttt tcattaagtc ctcctaaagg tgggttcaag
     4021 agaaagacaa aaactcaata tactcagaaa ggtacgctcg gagaaaacaa cgaattgatt
     4081 aatctagtaa ggagaatgat ataaatacta caaaatttta tttatgcagg aatgatgatg
     4141 atttttctaa tgttttctat tcataaaaac aaattaagga gtcttgattg tggctactag
     4201 attacgaaaa agtaggaggc aacgtggaac gcggtattgt ggatggggac agattggtca
     4261 acatagacag tccggttcca gaggtggtgt gggacaagct ggtaaacata agcatttctg
     4321 gatacgtact gttattgaag aaccagatca ttttggacat cctcaatcta aatctcatca
     4381 taaaattaaa gtaaaaaaat gggctaattt gagagatttg gatttattat acactgaaca
     4441 tatcaaaaac aaatctaatg aacgtgaaac tttagattta actactcttg gaatcaacaa
     4501 gttgttagga gcaggaaggg tcacaactaa aatgactgta aaggtagagc aaatttctcg
     4561 acgggcagtt gagaaaatta aagcaattgg aggaaatgta attttcaatg aacacagttc
     4621 agaatgaagg ttttattaga aaatcagtaa aaacaatatc gagatatatt ccccaagtag
     4681 aaaaaccaaa gaagaaaata tctttaaccg aaaaattcgt gtggtgtggt atagctgttt
     4741 ttgcatactt ggtaatgggt caggttcctc tctatggtgt cactgatgaa cctcaattcg
     4801 attttctcgc atttgcacgt gtgatttttg ccgcacaaca gggaactata ttagaacttg
     4861 gaataggacc tattgtaact gctgggttac ttatgcaatt gcttaagggg tcagacattt
     4921 taaaattaaa cttcaaaaat cctgatgatc gagccctctt tacttcagca actaaaattg
     4981 taactattat tgtgattatt gcagagggag gtctctatgg agcaagtgtc tatggaccct
     5041 tagttgcctc acctgacttt gtaccgattt tgatagcaca attgatattt acgagtctta
     5101 ttgtaatgct attagatgaa ttaatccaaa agggttgggg attaggatca gggataagtt
     5161 tgtttattat ggcgggggtg gctcaaacaa tcttatggag tgtatttagt cctgttgctg
     5221 gacctgatgg tcctgttgga attattcctt ttataattga ttctgcagca actggagatc
     5281 tttctgatac tttctttaga agtggtcagt taccaagtat ttttaccttt attttgactg
     5341 gaggtgtttt ggcccttctt gtatacacac agggaatcca tattgatatt ccaattgttt
     5401 caacgaagta tagaggcttt acagcagttt atccaattaa acttctttat gtttctaata
     5461 ttccagtaat tttagccaca gcattggttg caaatgcaat ttttatgggg caaatgttat
     5521 gggcaaatta taatccacaa aatgataatc cattttttaa ctggatagct cagtttgacc
     5581 ctggacaacc gcagactcct accggtggat tattgtatta tattaccact ccaaggggac
     5641 ttgatatagt tgttcaagac ccaattcggg cagtagtgaa tattatcttt gtaacagcga
     5701 ttgtgactgt atttggtcga ctatgggttg aactcggagg actctctgca aaagcggctg
     5761 cgaaaaatct cttagatgca gatgttcaaa tacctggttt cagaagatca cagaattctg
     5821 tacaaaatgt gttagataaa tatattccct ccgtaaccat aattggtggt attgcaattg
     5881 gactcttagc ttcggtttcg gatgtattag gtctttttgg tggtggaata ggcctcttgt
     5941 taatggtaga tattttagta aactattaca acctattaat ccgagaaaaa gttgatacta
     6001 ctatgcccaa attggcggct ttaattggta gaaaggacta aacgtgtcgt agtggttgga
     6061 ataccaggag ttggtaaaac cactatcgtt tctggtgtta aaagttcact agaagaagaa
     6121 ggaataaaaa ttaatttggc agaatttgga aaaataatgt tagaagaagc aactaagtta
     6181 aaaattaaaa atagagatga aatacgtcgc ctttctattg aagatcagaa aaaattgcaa
     6241 aatcttgctg ccaataaaat ttcaaaaata gaatcagatg taacaataat tgatacacat
     6301 ttatttataa ccactaaaga aggatattat cctggattac ctctagaatt attaaatcat
     6361 ctaaaaccaa ctaatctgat attattaatt gcaaatcctg aagaaattta tcatagaaga
     6421 caaaatgact catccagaga gagagatata atatcaatgg attctataaa gaatgaaatt
     6481 gacgtctcaa agatgctaat ttcttgctgt tcaatcatat caggtgctcc atttaaaatt
     6541 cttgcgaatc atgagggtgc tgtgacagat tgtactcaaa aaatggtttc attaatattg
     6601 gacacatgaa aaaaagacat gtaatatgta tttaggtgat cttattttac aaattcctgg
     6661 tcttactcca acatatacta aacatccaat ctttccagat ctattttctg ctatgattgt
     6721 agctgccata atatctattg gtctaaattt tgcgttcgcg attctgcgaa aaaaaactac
     6781 tgatattgat agactatcaa aagtgatgaa agaaacaaat gaatggagga aagaatacac
     6841 cgatgctatt agaaaacagg ataaagttag aattaacgag ttaaagaaaa agcaagccta
     6901 tgttaataaa atgagtttag aaatgcaaca acaacaaatg cgacctatga tgctttatat
     6961 gataccttca tttatgctct ggattcttgt atttccatct atttttggac aaactgtggc
     7021 tatttcacca ctgcagatcc cgtacataat gtgtacagag gaaaatgtta aattagatgc
     7081 tgccattgat gaaaatggtc agcccaaagg tgtatgcaat atccctggtg aagttttttt
     7141 atggggttgg tttcttatta cctcattggg aactagtggc cttgtcgcca agttaaccaa
     7201 aacctcactt cctagtatga cataaattgg ttacatctag ttataaaaat aaaatattaa
     7261 atggtattgt aatatctggt tggccagcag taggtaaaac cactattgct acagaattgg
     7321 ccaaagagtt tgggtacaaa cattacaatg gtggagatgt attaaaaatc ttggctgcag
     7381 agaaaggtta cttagtatct ggaaatgatt ggtgggatac tgaggaagca aaaaatttca
     7441 tggaagagag agaatcaaac ccttattttg atattgaagt agataggaaa ttggtagaaa
     7501 tagttagaat gggaaaagtt gtagtgacta gttatacatt gccatggtta gttccagaat
     7561 caattaaaat ctggttaaag gggtcaagaa gtaacagaat taaaagaatg gcaaaa
//
LOCUS       DATAFW010000087         7301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5857267, whole genome shotgun sequence.
ACCESSION   DATAFW010000087 DATAFW010000000
VERSION     DATAFW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5857267"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1801
                     /locus_tag="VHJ57_02095"
     CDS             356..1801
                     /locus_tag="VHJ57_02095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487358.1"
                     /translation="MDSRASEEVTIALIISVLSVLLIFSIIYVFSPDFFSKISLLEPI
                     QSLVLKLSEFLGYVAINYYQFIWIYIPLGIIGLWRWSVWIFKKSTSFLYKPKVPCSDT
                     TYHKYSIITPVYNEDPKIFEQAIRSWIKNSPNEIIGVIDQSDTRCIEIFNSIAKDYSF
                     MKIIIATKPGKRPALAEGIKEAKYKIVALVDSDTIWAENIRKSILSPFDDQLIGGVTT
                     KQHPIERKTIWQKITDIFWDMRNYYDLPAQTAMGQALSCLSGRTSLYRKDIIIEDLDN
                     FLNEIVLGRKKESGEDKCLTRIVQSKGWKTYYQSNAVIYSYAAPDFKTFWSQRLRWSR
                     NSHNSDLVSLWNGWAWKHPFLAFYIIDRFISIFTLFFGPIFFGISLYLNHYVLAVSIL
                     LLWMIGRGIKILPHLKRHPGDIILLPVYVVINFMIAIIKLYALVTIRDQKWIRSKNRK
                     SVTSTFKKIKNLILTTEIIGSLVFIVIFIIR"
     gene            1858..2067
                     /locus_tag="VHJ57_02100"
     CDS             1858..2067
                     /locus_tag="VHJ57_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487359.1"
                     /translation="MGRVNKGINCSIKGCTEQAERSMSESKGRMANDLDISSTSKRIY
                     LCRNHYKEWKKATKEDRETERARWK"
     gene            2094..4016
                     /locus_tag="VHJ57_02105"
     CDS             2094..4016
                     /locus_tag="VHJ57_02105"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX2487360.1"
                     /translation="MRLLQLHAEFMEYEPVEEELNDAEKNIVKSKIRLESLIVVFVSV
                     EKGDNKITIEKAIEEIKSYSENLKVNRLLIYPYSHLSSYLESPKNAFEIIKELETKSR
                     KILTEVNRAPFGWTKSFNIKVKGHPLAENFKVISNIQFPFIKGKDEISRKDDLKQHML
                     DMEPVSSALNSEEKQQSLWFILDTNGVLTPLEGFKIEKNSNLKKFLDYETSKRRAVDQ
                     QPPHVGLMKKMAIADYEPASDAGNMRYYPKGKLMKSLIEQFVTRNVLEYGGIEVETPI
                     MYDTKHPSMESYFNRFPARQYNIVSDNKQLFLRFAACFGQFLMAKDFHITYKNLPLKL
                     YELTRYSFRREKSGELVGLRRLRAFSMPDCHAFCKDIEQAKDECLKRFDLSLNVLNGF
                     DIKIDDIEMAIRFTETFYNENKEFVAEISRKFGKPILVEMWKDRFFYFIFKWEFNYVD
                     SLGKAAALSTDQIDVENGKRYKIEYIDEQGKRKNPIILHNSPSGAIERIIYTLLEKAA
                     KIKQQGERPQFPIWLSHTQVRIIPIKNEHLLFAENVENILSQSHIRTDIDDRQESLSK
                     RIREAETEWIPYIIVIGDKEAKSTILVIRDRKTGKQIESNIEELIESVRNEIRDKPFM
                     PLNLPKYLSKRPQIMV"
     gene            complement(4136..5416)
                     /locus_tag="VHJ57_02110"
     CDS             complement(4136..5416)
                     /locus_tag="VHJ57_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487361.1"
                     /translation="MELHGVTKDNIVKYPIIFFFTLLVFHLVFYSLSLQQIYGGAEQG
                     NTNSGPPYNGVDMHGFYTRTSQARDTNYDLPKNYFEESFKILSSNGLNHVRFLIFWES
                     FVKNPTEFIEELKTVANAADQYNIKVLYDNHQFHTSSYLNPQRGTGFPFSLFEADFAF
                     FYGSGGGTKYPTAKVWWTNWWDRNVRDTDANDGWVLQTEFFKKVLETVDNHTSTVGYE
                     ILSEPQVHDKNQWEKIGKYNTFMADFLRKYTDKTIAYSMNIPVSLQSPIELNPINLAK
                     MAPKNISNLVFKISVYGVPQENEYQESRLSNFVQAAELANVPLYVGEWNNVQRDKIID
                     EEGEIITFINETFSSINQSEANKLVSVFKDLDVWGSAFWQWRVDSHQVQNFNLINITN
                     GKIETTPYFDIVKTAYQTIYGDTQNTSSNTTTLA"
     gene            5554..>7301
                     /locus_tag="VHJ57_02115"
     CDS             5554..>7301
                     /locus_tag="VHJ57_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX2487362.1"
                     /translation="MSSIRNIIILGIVVSPSLLILILANNIEFSFAGNNQSPLTNNSE
                     FENTIMKNNPLKDDDICLRYENITKTIIVCNGTVDILTINDFFNNNSDILERTGEKEW
                     VLKSNILILENGTLNISGINTRLLKIDSDSSHNIAYSIISRGNLIIDNTNITSWNSTS
                     NEVPQLITPETPRAYVWTFWNSAGQTNITNSFFENLGYKGHSGTTGITFFSGDGSVIQ
                     NNTIINNYFGVRIANEVSNVTIKNNNILRSQFDGINLDSNTNNIDILENRIYENNLHA
                     IVCKKNCNNIKIMNNILENNIGIGILLERNSSINYVESNNIISNTMGMMVSESSENDI
                     IMNNFVGNGNGIFLKNTKNNLINSNNISNSNNYGINIYNNSTNNIIKFNNIKKSLNSG
                     VNIADYGTNNNKLVNNNIVEGPNFGLRLDRTSNNILEDNVIENNENDDFFIRQSKDNK
                     ILNSYFNYTAFLLNDEKSNMIITDTLNKIIDGNNITNTVYKQNNTIILTPNNNFTKLN
                     TIDLSVKPESSYVNITRVNYDLEKNKEYKRIDLVYPELGIDTKFILGGFNPGSQVLVK
                     VNKEIKDLITIGNNNST"
BASE COUNT         2798 a          996 c         1125 g         2382 t
ORIGIN      
        1 ttagaaatgt catagaaaag aacatcgtga cctagcttat gaaatccttt tcccgtggca
       61 gaaccaacta ctccggatcc aattatagaa atttttacca ttactgttcc acaaacctct
      121 ttttataaaa aaaaacccaa attaatatat aagtactact atctcttttt cactactata
      181 gttagaaggg attattttaa atattcttct ataaaatatt aatatgaatt ttaaatataa
      241 aatttatatc atacatgact gttactactc ttctttgagt tttttaataa caagttttac
      301 gatctatata taaattagaa tacactaata aaaaataatt tagatgtaca gattaatgga
      361 ttcaagagca tcagaggaag ttacgattgc tttaattatt tcagtattat ctgtacttct
      421 cattttttct ataatttacg ttttttcacc tgattttttt agtaaaatat ctttattaga
      481 acccattcag tctctagtct taaagctttc tgaatttttg ggatatgtgg caattaatta
      541 ctatcaattc atatggatat acataccact tggaattata gggctttgga gatggagtgt
      601 atggatcttt aaaaaatcta cttcattctt gtataaacct aaagtccctt gtagtgatac
      661 aacatatcac aaatacagca tcattactcc agtttataac gaagatccta aaatttttga
      721 acaggcaata agatcgtgga taaaaaattc acctaacgag ataatcggag taatagacca
      781 aagtgatacc cgttgtattg aaattttcaa ttctattgca aaagattatt catttatgaa
      841 gattataata gcaacaaagc ctggtaaaag gcctgcactt gctgaaggaa taaaagaggc
      901 aaaatataaa attgtcgctc ttgtagatag cgacacgata tgggcagaaa atattaggaa
      961 atcaatactt tctccttttg acgatcagct aataggaggt gttacgacaa aacaacatcc
     1021 tattgaacga aaaacaatat ggcagaaaat aacagacatt ttttgggata tgaggaatta
     1081 ttatgaccta cctgctcaga ctgcaatggg tcaggctctt tcgtgtctgt caggtagaac
     1141 ttccctatat agaaaagaca ttataattga agatttagat aactttctta atgaaatagt
     1201 cttaggaagg aagaaagaat ctggagaaga taaatgttta acaagaattg ttcaaagcaa
     1261 aggttggaaa acatactatc agagcaatgc tgtaatatat tcatatgcag ctcctgattt
     1321 taagactttt tggagtcaac gattgaggtg gtcaagaaat agtcataatt ctgatttagt
     1381 aagtttgtgg aatggttggg cttggaagca cccattttta gcgttttata taatagatag
     1441 atttatttcc atttttacat tattttttgg tccgatattt tttggtatat cactttattt
     1501 gaatcactat gtattagctg tatccattct acttttatgg atgataggac gaggaattaa
     1561 aattttacca catctaaaga gacatccggg ggatattata cttcttccag tctatgtagt
     1621 aatcaatttt atgattgcga taataaaatt atacgcctta gtaacaatca gagatcagaa
     1681 atggattaga tccaagaata ggaaatcagt tacctcaaca tttaaaaaga taaagaattt
     1741 aatactaaca actgaaataa tagggtcatt agtttttata gtaattttca taatacgtta
     1801 attctttaaa cctatctatc ggtatagctt ttatattcaa atataatttt actatagatg
     1861 ggtagagtaa acaaaggtat taactgtagc ataaaaggat gtaccgaaca agcagaaaga
     1921 tctatgagtg agagcaaagg aaggatggct aatgacttgg atatttcctc aactagtaaa
     1981 agaatctatt tatgtagaaa tcattataaa gagtggaaaa aagctacaaa agaagaccga
     2041 gaaacagaaa gagcaagatg gaaataaaaa attaggctaa ggtaagatga aaaatgcgtt
     2101 tattacaatt gcatgcagag tttatggagt atgagcctgt tgaagaggaa cttaatgatg
     2161 cagaaaaaaa tatagtaaaa tccaaaataa gattagaaag cttaatagtt gtttttgtgt
     2221 cagtagaaaa aggagataat aaaataacta tagaaaaagc aatagaagaa attaaaagtt
     2281 actcagaaaa tttaaaagtt aatagattac taatttatcc ttattctcac ttaagttctt
     2341 atttggaatc tccaaaaaat gcttttgaaa ttatcaaaga attggaaaca aaatcaagga
     2401 aaatactaac tgaggtaaat agagctccat ttggatggac aaagagcttc aatataaaag
     2461 taaaaggtca tccattagcg gaaaatttta aagtaatttc gaatattcaa ttccctttta
     2521 ttaaaggtaa ggacgagata tctcggaaag atgatctaaa acaacatatg cttgatatgg
     2581 agcctgtttc ttctgcttta aattcagaag aaaaacaaca atctctatgg ttcattctgg
     2641 atacaaatgg ggtattaaca ccattagaag gatttaaaat cgaaaaaaat agtaatttga
     2701 aaaagttttt agattatgaa acctcaaaaa gaagagctgt tgatcaacaa cctcctcatg
     2761 taggtcttat gaagaaaatg gctattgcag attatgaacc agcctctgat gcaggtaata
     2821 tgagatacta tccaaaggga aaattaatga aatcattgat agaacaattt gtaaccagaa
     2881 acgttcttga atatggcgga atagaagttg aaactcccat aatgtatgat acaaaacatc
     2941 catctatgga aagttatttt aatcggtttc ctgccagaca atataatata gtatcagata
     3001 acaaacaatt atttttacgg ttcgcagcat gttttggtca gtttttaatg gctaaggatt
     3061 ttcatattac atataaaaat ctcccattga aactttatga gttaacaaga tatagtttcc
     3121 gtagagaaaa aagtggagaa cttgtagggc taagaagatt aagagcattt agtatgcctg
     3181 actgtcatgc attttgtaaa gatatagaac aggcaaaaga cgaatgttta aaacgttttg
     3241 atctctctct aaatgtatta aacggatttg atataaaaat tgacgatata gaaatggcaa
     3301 taagatttac tgaaacattt tacaatgaaa acaaagaatt tgttgctgag atttctcgaa
     3361 aatttggaaa acctatatta gttgaaatgt ggaaagatag atttttttat tttattttta
     3421 aatgggaatt taattatgtt gatagcctag gaaaagcagc agctttatca acagaccaaa
     3481 ttgatgtaga aaatggaaaa agatacaaaa ttgaatatat agatgaacaa ggaaaaagaa
     3541 aaaatccaat aatattacat aattcaccta gcggagctat agaaagaatt atttataccc
     3601 tattagaaaa agctgctaaa attaaacaac aaggtgaaag acctcaattt cccatttggt
     3661 tatcccatac acaagttaga attataccta ttaagaatga acatttgcta tttgcagaaa
     3721 atgtagagaa tattctttcc caaagtcaca taagaacaga tatagatgac agacaagaat
     3781 ctcttagtaa aagaataagg gaagcagaaa cagaatggat tccatacata atcgttattg
     3841 gagataaaga agctaaatct acaattttgg taatacgtga tcgtaaaaca ggtaaacaaa
     3901 ttgaatctaa catagaagaa ttaatagaat ctgtaagaaa tgaaataaga gataaaccat
     3961 ttatgccatt aaatttaccc aaatatcttt cgaaaagacc acaaataatg gtttagtaaa
     4021 tgaatcatat cctttcaaat tttattatta acttatcaaa tatacattaa gtttattgaa
     4081 ttctgaactt catcctatgt tgttctattc acatttccta aattatatta ctagtttatg
     4141 ctaacgttgt agtattagaa gaggtatttt gcgtgtctcc ataaattgtc tggtatgcag
     4201 tttttactat atcaaaatag ggtgtagttt ctatctttcc gttggttata ttaatcaaat
     4261 taaagttttg cacttgatga gaatctactc tccattgcca aaacgcagat ccccatacat
     4321 caagatcctt aaacacagat actaatttat tggcttcact ctggttaata ctactaaatg
     4381 tttcattaat aaatgtgata atttctcctt cttcatcaat aatcttatct ctttgaacat
     4441 tattccattc tcctacgtat agtggaacgt ttgccagttc cgcagcttgt acaaaattac
     4501 tcaaccttga ttcttgatac tcattttctt gtgggactcc gtatacgctt attttaaata
     4561 cgagattact aatatttttt ggcgccattt ttgctagatt gattggattt aattcaatag
     4621 ggctttgtaa agatacagga atattcatac tataggcaat agttttatcg gtatattttc
     4681 taagaaaatc cgccataaat gtattatatt ttcctatttt ttcccattgg ttcttatcat
     4741 ggacctgtgg ctcactcaaa atttcatagc ccacagtact tgtatggtta tcaaccgttt
     4801 ctagaacttt tttgaagaat tctgtttgta aaacccagcc atcattagca tctgtatctc
     4861 gaacatttct atcccaccaa tttgtccacc atactttagc tgtcgggtat tttgtcccac
     4921 caccacttcc ataaaaaaat gcaaaatcgg cttcgaataa tgaaaaggga aaaccagtac
     4981 ctcgttgagg atttagataa gacgatgtat gaaattgatg gttatcatat agaactttaa
     5041 tattatattg atcagcagca tttgcaacag tctttaattc ttcaatgaac tctgtaggat
     5101 tttttacaaa agattcccag aaaattaaga atcgaacatg atttaatcca ttagatgaaa
     5161 gaattttaaa actctcttca aaataattct ttggaagatc ataatttgta tctcgagctt
     5221 gagaagttct cgtataaaag ccatgcatat caacaccatt atacggtgga ccgctattgg
     5281 tatttccctg ctcggcgcca ccatatatct gttgtaaaga taaagaataa aaaacaagat
     5341 gaaagactag taaagtaaag aagaatatga taggatattt aacaatatta tctttagtta
     5401 caccatgtaa ttccataaac caatataact tgattatttt tatataaatg tgtgtctctc
     5461 caaattttac tagaagtatt ttattttaaa aaaagtaata actatatgat tgtcgtcatt
     5521 tatatagcat attaatttaa catagtaacg caagtgtcga gtattagaaa tattataatt
     5581 ttaggtattg ttgtgtctcc atctttgtta attttaattt tagctaataa tatagaattt
     5641 tcatttgcag gtaataatca aagtccatta actaataatt ctgaatttga aaatacgatt
     5701 atgaaaaata atcctctaaa ggatgatgat atttgtttga gatatgaaaa cattacaaaa
     5761 actatcatag tttgtaatgg cacagtagat attcttacta ttaatgattt ttttaataat
     5821 aattctgata ttctagaaag gacaggagaa aaagaatggg tattgaagtc taatattttg
     5881 atactcgaaa acggaacatt aaatattagt ggtataaata ctcgtctact taaaatagat
     5941 tccgattctt cacataatat tgcatattct ataatttcaa gggggaattt aattatagat
     6001 aatacaaata taacctcatg gaattcaaca agtaatgagg ttcctcaatt aatcactcct
     6061 gaaactcccc gagcttatgt atggacattc tggaattcgg caggacaaac taatatcacg
     6121 aattcatttt ttgaaaatct gggttataaa ggacattcag gcactacagg aataacattt
     6181 ttttctggtg atggtagtgt aattcaaaac aatacgataa ttaataatta ttttggtgta
     6241 cgtatcgcaa atgaagtttc taatgttact ataaagaata acaatatttt aagaagtcaa
     6301 tttgatggta ttaatttgga ctcaaataca aacaatattg atatactaga aaatagaatt
     6361 tacgaaaata atttacatgc catagtgtgt aaaaaaaatt gtaataatat aaaaataatg
     6421 aataacatct tagaaaataa tataggtatt ggaattctac ttgaaaggaa ttctagtata
     6481 aattatgttg aatcaaacaa tataatatcc aacacaatgg gaatgatggt ttctgaatca
     6541 tcggaaaatg atataataat gaataacttt gtaggaaatg gtaatggaat tttcctgaaa
     6601 aatacaaaga acaatctaat taattcaaat aatataagca acagcaataa ctatgggata
     6661 aacatataca ataattctac taataatata attaaattta ataatataaa aaagtccttg
     6721 aatagtggag taaatattgc agattatgga acaaataaca ataaattggt aaataacaat
     6781 attgttgaag gccctaactt tggcttaaga cttgatagaa catctaataa tatattagaa
     6841 gataatgtaa tagaaaataa tgaaaatgac gatttcttta taagacaatc caaagataac
     6901 aagatattaa attcttattt taattatact gcattcttgc taaatgatga aaaaagtaac
     6961 atgataataa ccgatacatt gaataagatt attgatggaa ataatattac taatacagtc
     7021 tacaaacaaa ataacacaat aatacttact cctaataata attttacaaa attaaatact
     7081 attgatctgt cggtaaaacc agaatcatcc tatgtaaata tcaccagagt aaactatgat
     7141 ttagaaaaaa ataaagagta taaaaggatt gatttagtat atcccgaatt aggaattgat
     7201 acaaaattta ttttaggagg atttaatcca ggaagccaag tattggtaaa ggtgaacaaa
     7261 gagataaaag atctgataac tatcggtaat aacaacagta c
//
LOCUS       DATAFW010000088         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_923422, whole genome shotgun sequence.
ACCESSION   DATAFW010000088 DATAFW010000000
VERSION     DATAFW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923422"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VHJ57_02120"
     CDS             complement(<1..175)
                     /locus_tag="VHJ57_02120"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX2487363.1"
                     /translation="MTNDKNKNQKSIVKKLVVNDIVSMKNNEKISVITSYDYWSAKIC
                     DNTGIDIILVGDSG"
     gene            complement(191..937)
                     /locus_tag="VHJ57_02125"
     CDS             complement(191..937)
                     /locus_tag="VHJ57_02125"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HEX2487364.1"
                     /translation="MIKANHIPSNHPRAKSLAIRQLLANGFKSGLVVSEGLIAHGRGE
                     AFDYLIGEKTSVYAFDAIKAASSLLLLAKRPVISVNGNTAALCPKEIVDLANETRSSI
                     EVNLFHRNKRRENLIAETLKKNGANIVLGVGQKEFLKIPELTSNRRHVDPEGIFVADV
                     VFVPLEDGDRTKALINMKKKVITVDLNPLSRTAQSATISIIDNIVRAIPELVKTSKQL
                     KQKSQNTLLKILKNFNNRYNIDNSLKIIRR"
     gene            complement(924..1865)
                     /locus_tag="VHJ57_02130"
     CDS             complement(924..1865)
                     /locus_tag="VHJ57_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487365.1"
                     /translation="MTSSEIGDLVARCTAFSPGHITGFFEKPKSKNKNTDPLFYGSKG
                     AGFSFKKGVTTSVELYKSKHKWYQIYINKSLTNNAEVSEWVTQYYLENFDITYYIKIN
                     HFIDIPIGYGLGTSGAAALSLSYALNGVLGKKFLREQAAQIAHIAEIDCNTGLGTVIS
                     EFYGGLEIRTSFGAPGIGQVTKIELNNYKAIILCIGPLSTKYMLSNSSNKANILGRKM
                     VERLLLSIDVNSFLRMSHEFADFLGLTKGLCAKPIEELNNLGIDCSIGMFGETIFTLV
                     PPNEISLVSSVLKKFPGTLIVSDIDNRGALIENFDQS"
BASE COUNT          654 a          356 c          267 g          731 t
ORIGIN      
        1 ctccactgtc accaaccagt ataatatcga tacctgtatt atcacatatt tttgcactcc
       61 aataatcata ggatgtaatg accgaaattt tttcattatt tttcatagat acaatatcgt
      121 tcacaaccaa tttctttact atcgattttt gatttttatt tttatcatta gtcatttact
      181 actcacattt tcatctacgt attattttta atgaattatc aatattatat ctattattaa
      241 aattcttcaa tattttcaat agagtgttct gactcttttg tttcaattgt tttgaggttt
      301 taacaagttc tggtatagcc cttacaatat tatctattat acttattgtt gcactctgtg
      361 cagttcgtga aagaggattc agatcaactg taattacttt ttttttcata ttaattaatg
      421 cctttgtacg gtcaccatct tctaagggta caaaaacgac atcagccaca aatattcctt
      481 caggatcaac atgtctacga ttactagtca gctctggtat ttttagaaat tctttttgac
      541 ctactccaag aacaatgttc gctccatttt tctttagagt ttcggcgatt aaattttctc
      601 gtcttttatt tcgatgaaat agattaactt ctattgatga tctagtttca tttgctaaat
      661 caacaatctc tttaggacat aatgctgctg tatttccatt tacagatatt acaggtctct
      721 tagcaagtaa caataaactt gatgctgctt taattgcatc aaaagcataa acagaggttt
      781 tttcaccaat aagataatcg aatgcttctc ctctaccatg ggctattagc ccttcactaa
      841 caaccaggcc agatttgaaa ccattagcaa gtaattgtct gattgcaaga gattttgctc
      901 tgggatgatt ggatggaata tgattagctt tgatcaaagt tttctatcaa cgctcctcga
      961 ttatcaatat cagaaactat caaagtaccc ggaaacttct tcaatactga ggaaactaga
     1021 gaaatctcgt taggaggaac caatgtgaaa attgtttctc caaacatacc tatactacaa
     1081 tctattccaa gattgtttaa ttcttctatt ggttttgcac acaatccttt tgtcaatcct
     1141 aaaaaatccg caaattcatg agacatcctt aaaaaagaat taacatcgat tgataataat
     1201 aatcgttcta ccatctttcg accaagaata ttagccttat ttgaagaatt agaaagcata
     1261 tattttgtag atagagggcc aatacaaaga ataatagctt tataattatt taattctatt
     1321 ttagttactt gacctattcc tggagctcca aaactagtcc ttatttctag tccaccataa
     1381 aattctgaga taacagtacc taaacctgta ttgcaatcaa tttctgctat atgcgcaatt
     1441 tgagcggctt gttcccttaa gaatttttta ccaaggactc cattcaaagc ataagaaagg
     1501 ctcaaagcag ctgcaccact tgttcccaat ccataaccaa taggaatatc tataaaatgg
     1561 tttatcttga tataataggt aatatcaaaa ttttcaagat aatactgtgt tacccactct
     1621 gaaacttctg cattatttgt taatgattta ttaatataga tctgatacca tttatgctta
     1681 gatttgtaaa gttctacaga agtagttact ccttttttaa aggaaaaacc agccccttta
     1741 gatccataaa aaagtggatc tgtgttttta tttttacttt ttggtttttc aaaaaagcct
     1801 gtgatatgac caggactgaa tgctgtacat ctagcaacaa ggtcaccaat ttcagacgag
     1861 gtcatagact aaaagtaaat ataacatgaa aatataaaaa tatcgatact aacttataat
     1921 gtaaaaggta cttttataaa gtcgattttt atatattata attaaattgt ctattatatg
     1981 gtaaacttta atttatcgca attttctc
//
LOCUS       DATAFW010000089         6334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_747997, whole genome shotgun sequence.
ACCESSION   DATAFW010000089 DATAFW010000000
VERSION     DATAFW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747997"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /gene="leuS"
                     /locus_tag="VHJ57_02135"
     CDS             <1..3034
                     /gene="leuS"
                     /locus_tag="VHJ57_02135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX2487366.1"
                     /translation="REINDKSINWKELEAKWKSRWKEEHLFEADYDNTNKKKYFITVA
                     FPYPNSPQHIGHGRTYSLADVHARYMRMKGYNVLFPMGFHYTGTPILAMSKRVIAEDQ
                     DLIETFKNLYNVPENIIKSFIEPENIAKFFHNEIKLGMQDMGYSIDWRREFTTIDTLY
                     SKFITWQFKTLQKKGLIVQGSHPVGWCPYDKNPVSQHDTIGDVEPEFSEYVLIKFRLA
                     DNDIILPTATLRPETIFGVTNLWVNPDIEYVLIDLDNNVEQWIVTKEALTKLEFLKHS
                     IKINRTIKGTELIGKFAKDIIRNINIPILPAKFVKPDTGTGVVMSVPGHAPYDFQAII
                     DLKKERNLLSYNIDLSQINPIKIIEIQEANLNGNNLISCVEDAISKLKINNQLDLGLE
                     KATNEVYHLEYYQGKMLPNTGKYSGMNVQLAKEEIKNYLLATKNLAEIFYEFVNKPIK
                     CRCGNECFVKLLNDQWFINYKDKEWKHKVHNYIDKLSIIPEELRQEFHNVVDWLKERA
                     CARKSGLGTKLPWDHEWIIESLSDSVIYMAYYIISKFVNEQKYSMKTSLNNIDDSFFD
                     YILLGIGNVHDVAKNCNLSISLLQEIRNEFIYFYPVDSRHSGRDLVPNHLTFFIFNHI
                     AIFDKKFWPCQIVVNGSVLMEGKKMSKSLGNIIPLRTAIQEYGADTIRLAILISSELL
                     QDADFSFDTARSIRIKLLEFYTLSQQILNSNFYNKVLNQNLSIDELLYLDIFENEYPL
                     ELEDRWLISKLQRLINNVTKSMETLRIREALHNILYVFDQHIHSYKKRINAKKRSDDN
                     IVNKILILCFSIRIKLLSPFAPFISEEIWNSLGNRSSIVSSKWPIVFNERIDPLSEES
                     GEFIKKLTLDISNIIKVTKKLPSTIYIYCSSSDKQKIYQKILKIITLTNERNFGNIMK
                     KLIQDPETSYAKEDPAFVKKTVEDILSESVESRSNRLNLNSLDEISIIKDSEKLISNE
                     IEFENIQIIVYSEEEKEKFDPKSKSRFSRPYKPAVYLE"
     gene            complement(3022..3219)
                     /locus_tag="VHJ57_02140"
     CDS             complement(3022..3219)
                     /locus_tag="VHJ57_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487367.1"
                     /translation="MPGSARAGIAILVLGAILLGIGYYQDRGALSLYGLIMSICGFSL
                     YMISSIYISRKSNKTKGSYSK"
     gene            3279..4325
                     /locus_tag="VHJ57_02145"
     CDS             3279..4325
                     /locus_tag="VHJ57_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX2487368.1"
                     /translation="MDSDIVSMKIAVVGIGVAGAYLMNQLSNNHDIHVVGFERMKEEE
                     HDAVCAWATSKNVMVDYTKKCNLNFDNYILHDGKNMSVDLGESKHIDIGLKGMVSYDK
                     LKLIQDMIKGTEIKYGKAPKKENLEADFDLIVDSTGFHRNYLPKLDYEMWVPCVQYKV
                     KYDNNNIPYDDFYLKGFPSMSGYFWYFPLENGYAHIGAGDFTRKNHNIFVENFLKKYK
                     CEIIKKNGRPVRISPPSKSEPFYDGKKSIGVGESIGTVYPLLGEGIIPSTICADLFIN
                     NLNNFKKYREQVLKHFSIYPLVFKFILMKITGTFNLVKNSLDLLRIYHHMKNNQDRYG
                     MEVKIAELMKLSTL"
     gene            4409..4849
                     /gene="pfdA"
                     /locus_tag="VHJ57_02150"
     CDS             4409..4849
                     /gene="pfdA"
                     /locus_tag="VHJ57_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HEX2487369.1"
                     /translation="MSNKANTNKSAIEQRVNELVQHSRTLELYLNEVLEREATVTKLI
                     EEARLASYALQNIPLDKENEALMPIGVGVYLKTNITSIDKLLVNIGAGVTIEKTHEDT
                     LHYIESRIKEFEIGLTQLREQKKQITSQLEQIQHQVNSMLQQTS"
     gene            4925..>6334
                     /gene="ftsY"
                     /locus_tag="VHJ57_02155"
     CDS             4925..>6334
                     /gene="ftsY"
                     /locus_tag="VHJ57_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX2487370.1"
                     /translation="MFDKLKKAFSNIISDISKKEISEKDLENTLFDLEIALLESDVAQ
                     EVIDDISASLKNKLLGLSVEKDKTPEELIQFHVQKSIYEILTKSGTLDLVKEINSKKE
                     NKEGPFVVVFLGINGTGKTTTVAKVANLLHKNGLSVVLAASDTHRAGAIEQLGEHANR
                     LSLKMIHQRYGADPSAVARDAVEYGKKHRVDAILIDTAGRMQTAKNLMDEISKIVQVV
                     KPDLKIFIGDSLAGNDAIYQAKEFFAYTKFDASILTKTDADAKGGAIISITYLTSKPI
                     IYLGIGQNYDDIIPFDLNTFIDSLFGSQISEEIKNNYSSNIVNESRNNEKLSDIKSKN
                     IITNEPINNIEKLYQNKPSSSSSSSSNLEPIINNINNNDIPISKVEVKEEEKKVDDLR
                     NNNIINLENDSQFGKSEKMTEKNPSNNDNDREKHFITKEISENKDKKKKGFFSSLFSK
                     RKSQINSDKVSSTDDQKNKQ"
BASE COUNT         2499 a          817 c          965 g         2053 t
ORIGIN      
        1 tagagaaata aatgataaga gtattaattg gaaagaactg gaagcaaaat ggaagtctag
       61 atggaaagaa gaacatcttt ttgaagccga ttacgataat actaataaaa aaaaatattt
      121 cataactgtt gcatttccct accctaattc tccacaacat ataggacatg ggagaacata
      181 ttcactagct gatgttcatg ctagatatat gagaatgaaa ggatataatg ttttatttcc
      241 gatgggattt cactatactg gaactcctat cttagctatg tcaaaaagag taatcgcaga
      301 agatcaagat ttaattgaaa cttttaaaaa tttatataat gtgcctgaaa atatcatcaa
      361 atcatttata gaaccagaaa atattgcaaa gttttttcat aatgagataa aattaggaat
      421 gcaagatatg ggttattcta tcgattggcg acgagaattt acaaccattg atacattata
      481 ttcgaaattc attacttggc aatttaaaac attacaaaaa aaagggttaa ttgtacaagg
      541 ttctcatcct gtaggctggt gtccctatga taaaaatcct gtaagtcaac atgatactat
      601 tggagatgta gaaccagaat ttagtgaata tgtcttaata aaatttaggt tagctgataa
      661 tgatataata cttccgactg ctactttaag acctgaaact atatttggtg tcacaaatct
      721 ttgggtaaat cctgatattg aatacgttct aatagatcta gataataatg ttgaacaatg
      781 gattgttact aaagaggcat taacaaaatt agaatttctt aaacatagta taaaaattaa
      841 tagaacaatc aaaggaactg aattaattgg taaatttgca aaagatatta ttagaaatat
      901 aaatattcct attctcccgg caaaatttgt aaaacctgat acaggaactg gagtagtaat
      961 gtcagtccct ggtcacgctc catatgattt tcaagctatt attgacttaa aaaaggaaag
     1021 aaatttacta tcatataata ttgacttatc acaaatcaat ccaataaaaa ttatagaaat
     1081 tcaagaagct aatttaaatg gcaataattt aatatcatgt gtagaagatg caatatcaaa
     1141 gttgaaaatt aacaatcaat tagatttagg tctggaaaag gcaacaaatg aagtctatca
     1201 tctggaatat taccaaggca agatgttacc taatacagga aaatattccg gaatgaatgt
     1261 tcaattagct aaagaagaaa taaaaaatta tttgcttgct acaaaaaact tagctgaaat
     1321 tttttatgaa ttcgttaata agccaattaa atgtagatgt gggaatgaat gttttgtcaa
     1381 gttactaaat gatcaatggt ttattaatta taaagataaa gaatggaagc ataaagtaca
     1441 taattatata gataaattaa gtattattcc tgaagagctt agacaagaat ttcacaacgt
     1501 agttgactgg ttaaaggaaa gagcttgtgc aaggaaatca ggtcttggaa cgaaacttcc
     1561 atgggatcat gaatggataa tagaaagctt atctgattca gttatttaca tggcttatta
     1621 tataatatca aagttcgtta acgaacaaaa atactcaatg aaaacaagtc taaataatat
     1681 tgatgactct ttttttgact atatactatt aggtattgga aatgttcatg atgtagcaaa
     1741 aaattgtaat ctttccatat cattattaca agaaatcaga aatgaattta tttattttta
     1801 tccagttgat tctcgacatt ctggacgaga tttggttccg aatcacctta ctttctttat
     1861 ttttaatcat attgctattt ttgataagaa gttttggcca tgccagatag tagtcaatgg
     1921 ttctgtttta atggaaggaa aaaagatgtc aaaatcatta ggaaatataa taccattaag
     1981 aacagcaata caagaatatg gagcagatac aattagacta gcaatattga tttcatctga
     2041 gcttcttcag gatgcagatt tttcttttga tactgcgaga tctattagaa taaaattatt
     2101 agaattctac actctttcac aacagatatt gaattctaat ttttacaata aagtattaaa
     2161 ccaaaattta tcaatagatg aattacttta tctagatata tttgaaaatg aatatccttt
     2221 agagttagaa gataggtggt taataagtaa attacaaagg ctaataaata atgttacaaa
     2281 atcaatggaa acattacgta tacgtgaagc tcttcataat attttgtatg ttttcgatca
     2341 acatattcat tcgtataaaa aaagaattaa tgctaaaaaa cgaagtgatg ataatatagt
     2401 caataaaata ctaattcttt gtttttctat tagaataaaa ttattatccc catttgctcc
     2461 atttatatct gaagaaattt ggaatagttt aggtaatcga tcttcaattg tttcttcaaa
     2521 atggccgatt gttttcaatg aaaggattga tccattatca gaggaatcgg gtgaattcat
     2581 aaaaaaatta acattagata tatctaatat tattaaagta actaaaaaat tacctagtac
     2641 tatatatata tattgttctt cttctgataa acaaaaaatt tatcagaaaa ttcttaaaat
     2701 aattacttta actaatgaaa gaaattttgg aaatataatg aaaaaattaa tacaagatcc
     2761 tgaaacttct tatgcaaaag aggatcctgc ttttgttaaa aaaactgtag aagatattct
     2821 ttctgaatct gttgaatcaa gaagtaacag attaaatttg aactcattgg atgaaatttc
     2881 tataataaaa gattcagaga agctaatatc taatgaaatc gaatttgaaa atattcaaat
     2941 aattgtttac tcagaagaag aaaaggaaaa atttgatcca aaatcaaaat ctagattttc
     3001 aagaccttat aaacctgctg tctatttaga ataagatcct tttgttttat tactttttct
     3061 tgaaatataa atcgatgaaa tcatatacaa gctaaaccca cagattgaca ttattaatcc
     3121 atataaagaa agtgctcctc tatcctgata gtatcctata ccgagaagta tcgctcctaa
     3181 taccaaaata gctatacctg cccttgcaga acctggcatt tcttttttct ttttcactag
     3241 tgatacatgt ttaatttttt tgcaatatat ttttattttt ggatagtgat attgtatcaa
     3301 tgaaaattgc tgtagtggga attggggttg caggagcata tttaatgaat caacttagta
     3361 ataatcatga tattcatgta gttggatttg aaagaatgaa agaagaggaa catgacgcag
     3421 tttgcgcttg ggctaccagc aaaaatgtta tggtagacta tacaaaaaaa tgtaacctaa
     3481 attttgataa ctatatctta catgatggta aaaacatgag cgtagatctt ggtgaaagca
     3541 agcatattga tatcggatta aaaggtatgg ttagttatga taaattaaaa cttattcaag
     3601 atatgataaa aggaacagaa attaaatatg gaaaagcacc aaaaaaggaa aatttagaag
     3661 ctgactttga tctcattgta gattctactg gttttcacag aaattatctc cctaaacttg
     3721 actacgaaat gtgggtacct tgtgttcaat ataaagtcaa atatgataat aataatatcc
     3781 catatgatga tttttatctt aaaggatttc cttctatgtc tggctatttc tggtattttc
     3841 cattagaaaa cggatatgca catataggtg ctggtgattt tacaagaaaa aatcacaata
     3901 tttttgttga aaattttctg aagaaataca aatgtgaaat aattaaaaaa aatggtagac
     3961 ctgtacgaat tagtccacca tctaaatcag aaccatttta cgatggtaaa aagtctatag
     4021 gcgttggaga atctattggt acagtttatc cattacttgg tgaaggcata atcccttcaa
     4081 caatttgtgc ggatctcttt ataaataatt tgaataattt taaaaaatat agagaacaag
     4141 tccttaaaca ttttagtata tatccattag ttttcaagtt tattttgatg aaaattacag
     4201 gtacttttaa tcttgtaaaa aactctttag atctacttag aatatatcat catatgaaaa
     4261 ataatcaaga tcgatatgga atggaagtaa agatagcgga attgatgaaa ctctctactc
     4321 tttagctata atttgaaaaa tttcaatatt ttttatcttt ttctctttga gtcacagtta
     4381 taaatggatt taaaatactt caatatagat gagcaataaa gctaatacga ataaatcagc
     4441 aattgaacaa agagtcaatg agcttgttca gcattccaga acactagaac tttatcttaa
     4501 tgaagtatta gaaagagaag ctacagttac aaaattaatt gaagaagctc gtcttgcctc
     4561 atatgctctc caaaatattc ctctagataa agagaatgaa gcattaatgc caattggagt
     4621 gggagtatac ttgaaaacaa atattacttc aattgataaa ttattggtta atattggtgc
     4681 aggagttaca atagaaaaaa cacatgaaga tactctacac tatatagagt caagaatcaa
     4741 ggaatttgag attggcttaa ctcaattaag agaacagaaa aaacaaataa cttctcaact
     4801 tgaacagatc caacaccaag ttaattctat gttacaacaa acttcataat atattgataa
     4861 atgcaaaaaa taaaacataa tacatattgt gatttaaatt attttttggg tatgatgagt
     4921 atatatgttt gataaattaa aaaaagcttt ttcaaatatt atttctgata tcagtaaaaa
     4981 agaaatttct gaaaaagacc ttgaaaatac tctattcgat cttgaaattg cattgttaga
     5041 aagtgatgta gcccaagaag ttatagatga tatctccgct agtttgaaaa ataaattatt
     5101 gggtctctct gttgaaaaag ataaaacacc agaagaatta attcaattcc atgtccaaaa
     5161 atctatttat gaaattctta ctaaatccgg gactctggac ttagttaagg aaattaattc
     5221 caaaaaagaa aataaagagg gcccatttgt agtagttttt ctaggaatta atggaactgg
     5281 aaaaaccact actgtagcca aagttgctaa tcttctacat aaaaatgggt tgtctgttgt
     5341 attagcagca agtgatactc acagagcagg tgctatagaa caactgggtg aacatgctaa
     5401 tcgattatca ctgaaaatga ttcatcaaag atatggagcg gatccatctg cggttgcaag
     5461 agatgcagtt gaatatggaa aaaagcacag agtagatgca attttaattg atactgctgg
     5521 aagaatgcaa actgcaaaaa atcttatgga tgaaattagt aaaattgttc aggttgttaa
     5581 accagactta aaaattttca taggagattc tttagcagga aatgatgcaa tatatcaagc
     5641 aaaagaattc tttgcctata ctaaatttga tgcgtcaatt ttaacaaaaa cagatgcgga
     5701 tgcaaaggga ggggctatca tttcaattac atatctcact tctaaaccta ttatttatct
     5761 tggaataggt caaaattatg atgatataat tccatttgat cttaatactt tcatagactc
     5821 attgtttggt tctcaaattt cagaagaaat taaaaataac tatagttcta atatagtaaa
     5881 tgaaagtcga aataatgaaa agttatcaga tatcaaatcc aaaaatatca ttactaacga
     5941 acctattaat aatattgaaa aattatatca aaataaacct tcttcttctt cttcttcatc
     6001 ctccaattta gaacctatta ttaataatat aaataataat gatattccaa tatctaaagt
     6061 tgaagtaaaa gaagaagaga aaaaagttga tgacttaaga aataataata ttattaattt
     6121 agaaaatgat tcacaatttg gtaaatctga aaagatgact gaaaaaaatc catctaataa
     6181 tgataatgat agggagaaac attttattac caaagaaata tcagaaaaca aagataaaaa
     6241 gaaaaaaggt ttcttttctt cattatttag taaacggaaa tcccaaataa atagcgataa
     6301 agtttcatct acagatgatc aaaaaaataa acaa
//
LOCUS       DATAFW010000090         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_748669, whole genome shotgun sequence.
ACCESSION   DATAFW010000090 DATAFW010000000
VERSION     DATAFW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748669"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VHJ57_02160"
     CDS             complement(<1..635)
                     /locus_tag="VHJ57_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX2487371.1"
                     /translation="MAASLHKENSLVRLFRWIYAGVDRTIFMGLKFTFPTRFVSPLGF
                     LGMLTFVLFIILGISGALLMLWYEPILDRAWDSVENINDTLPYGFHLRNIHYHASNAM
                     VMMAILHMYYQYFSGRYKIRNEILWVTGILIGVLTILEAFTGYDIIFSERAELAISIA
                     ASLTNSIPIIGPDMRDAFFGSGFHDFVLRFYAFHVFLLPIALIGLMIVHFPR"
     gene            complement(616..1227)
                     /locus_tag="VHJ57_02165"
     CDS             complement(616..1227)
                     /locus_tag="VHJ57_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX2487372.1"
                     /translation="MSEQPQSRGDDRISRRDFLKLLAAAGTVLTFTPFVDWGKFLPNP
                     SASTNEKSKVELPDGSQANVKTFKVNHSEAIIYPISEDPALNKEAFRTWQFIRLPVEL
                     GGDKNDASAFRAFSMVCLHLWCLWKYWPGEGRKRGECPCHGSLYDPATGQAFAGPAAL
                     QSPPSNVLPNLDLEVDKDGYLWILPPNWTVNGNGIVGYGRFIA"
     gene            complement(1315..2310)
                     /locus_tag="VHJ57_02170"
     CDS             complement(1315..2310)
                     /locus_tag="VHJ57_02170"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX2487373.1"
                     /translation="MFPIHDDTPRLHGRPYVNYSLIALNVIIFIWEVLRTNFFRNELI
                     VNEIFFTYGTVPDLVFQGDFFAIVTSMFLHGGVAHIIGNMVFLYIFGDNIEDRFGHFK
                     YLILYLFWGIAAGIIHSFYAVSVGGGDIPAVGASGAISGVLGAYLILFPRAKIFTIII
                     AFFVTTIRIPALAYIPIWFILQVIFSILNPEGGVAYLAHIGGFVVGAGVSYLTKSFFP
                     SFFQGHIDGGSNYLKKSRGYSRRMYQDNPRHSISQPDIIEGDNYYEILVELKGLRNIH
                     NLRAMYEPGTHSLQIFSEGSLNPIVSIPLREEMRISIIQDVSYINGILRVRMLKS"
     gene            complement(2339..2509)
                     /locus_tag="VHJ57_02175"
     CDS             complement(2339..2509)
                     /locus_tag="VHJ57_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487374.1"
                     /translation="MGFSDILKRKKDKEDSRMKCKYCNIVFQDKEHLSRHNRKAHGKK
                     DDFLPSSNPFQV"
     gene            complement(2585..2815)
                     /locus_tag="VHJ57_02180"
     CDS             complement(2585..2815)
                     /locus_tag="VHJ57_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX2487375.1"
                     /translation="MPGAFVLLNTELGSEDSIVKELKNLEGVKEVFQVYGVYDIIAKV
                     ESDTMDKVKEIITWKLRKLPGVKSTLTMIVME"
     gene            complement(2874..3680)
                     /locus_tag="VHJ57_02185"
     CDS             complement(2874..3680)
                     /locus_tag="VHJ57_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX2487376.1"
                     /translation="MPNNIPEKTESLFFELAGDLRLSMLLKLSKHKYRLSQLSTELDA
                     TMQEAHRNIIRLVESGLVYKDGEGDLGLTSYGKAIVFLIPGYEFLLQNKQYFIEHSIE
                     ELPLKFIQRIGSLQNSEIVLGVMANLQRWKTLYWNSKVYIREIMSQVPLDLIETVSNK
                     IETGIKFSYIFSKDTIIPKGRGELLQKIGWVNQISKGMVERKMINKVTIMTIFNEQEA
                     CLLFPNLKGEPDLNIMFYSRDQNFHDWCKDFFDYQWELAGPFDDKKLSET"
     gene            complement(3779..>4022)
                     /locus_tag="VHJ57_02190"
     CDS             complement(3779..>4022)
                     /locus_tag="VHJ57_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX2487377.1"
                     /translation="FGEITSGGLREDNLQKLEKRMVLEGLQKSDYDWYLDLRRYGSVP
                     HGGFGLGIERLMRWILNIPDIKDTLPFPRTLRRIYP"
BASE COUNT         1403 a          764 c          577 g         1278 t
ORIGIN      
        1 cggggaaaat gaacaatcat caaacctatc aatgcaattg gaagtaaaaa tacatgaaat
       61 gcataaaagc gtaatacaaa atcatgaaat cccgacccaa agaatgcatc tcgcatatca
      121 ggtcctataa taggtattga attagtaagt gaagcagcta tagatatagc cagttctgct
      181 ctttcactaa aaataatatc ataccctgta aaagcttcta aaattgtaag tactcctata
      241 agaattccag tgacccaaag tatttcattt ctaattttat atctaccact aaaatactga
      301 taatacatat ggagaattgc catcataacc atagcgttag atgcatggta gtgaatattc
      361 ctgaggtgga aaccatatgg tagagtatca tttatatttt caacgctatc ccaagctcta
      421 tcaagaattg gttcatacca taacataaga agagcaccag atataccaag aattatgaag
      481 agtacaaaag tgagcattcc tagaaagccc aacgggctaa caaaccgagt aggaaaggtg
      541 aattttaaac ccatgaaaat tgttctatca actccggcat agatccaccg gaagagtcga
      601 actagactat tttctttatg caatgaagcg gccatatccc actatcccat ttccgtttac
      661 agtccaatta ggaggtaata tccataaata gccatcttta tctacttcca aatcaaggtt
      721 tggaagtaca tttgaagggg gagattgaag ggcggcaggt cctgcaaagg cctgtcctgt
      781 agcaggatca tacaaacttc catgacatgg acattcaccc ctctttcttc cttcaccagg
      841 ccaatacttc cataggcacc aaagatgtaa acaaaccatg ctaaaggcgc gaaaggctga
      901 agcatcattc ttatctcccc ccaattcaac tggaagacgt atgaattgcc aagttctaaa
      961 tgcttcttta ttgagagcgg gatcttcaga tatggggtat ataatagctt ctgagtgatt
     1021 aaccttaaag gtttttacat tagcttgaga accatcaggt aattcaactt tcgatttttc
     1081 atttgttgaa gcacttggat taggcaaaaa cttaccccag tcaacgaaag gggtaaatgt
     1141 caacacagta ccggctgctg ccaataactt taaaaaatct ctacgagaaa ttctatcatc
     1201 tccccttgat tggggctgtt cagacattat aataacagaa gaaaaaatat attataaatc
     1261 tttgttccaa tatagaaata ttttagttga tggctaatta atacagaatt atcgttaact
     1321 ttttaacatt ctgactctaa gaattccatt tatgtatgaa acatcctgaa ttatagatat
     1381 gcgcatttct tctcttagtg gaatagacac aatagggtta agagatccct ccgaaaaaat
     1441 ttgtaaacta tgtgtacctg gttcatacat agctcgtaaa ttatggatat ttctcaaacc
     1501 ttttagctct actaaaattt cgtaataatt atcaccctcg ataatatctg gttgagagat
     1561 tgaatgtcta ggattatcct gatacattcg tctagaatag cccctagatt tttttagata
     1621 attagaacca ccatcaatat gtccttgaaa aaacgaagga aaaaatgact ttgttaggta
     1681 actaacgcca gcaccgacta caaaacctcc gatatgagct aaatatgcta ctccaccttc
     1741 cggattcaaa atactgaata ttacttgtag aataaaccag ataggtatgt aagctagagc
     1801 cggaattcga atagttgtaa caaaaaaagc tataataatt gtaaatattt tcgccctagg
     1861 aaaaagtatt aaataagctc ctaaaactcc tgatattgcc cctgacgcac ctactgcggg
     1921 aatatcaccg ccgcccactg aaactgcata aaaactatga attattccag cggctattcc
     1981 ccagaacaga tacaaaataa gatacttgaa gtgtccaaat ctatcctcaa tattgtcacc
     2041 aaaaatatat agaaagacca tatttcctat tatatgagca actcctccat ggagaaacat
     2101 agaggtaact atagcaaaaa agtcaccctg aaagacaaga tctgggacag taccataagt
     2161 aaaaaagatt tcattgacta ttaactcgtt tctaaaaaaa tttgttctta atacctccca
     2221 gataaatatt ataacattta aggctataag tgagtagttt acataaggtc gtccatgaag
     2281 acgaggggta tcatcatgaa taggaaacat ttactaaaat ggatccaagc gtcgagtgtt
     2341 aaacctgaaa aggattacta cttggtaaaa aatcatcttt ctttccatgc gcctttctat
     2401 tatgtcggga aagatgctct ttgtcttgga aaacaatatt gcaatattta catttcatcc
     2461 tactatcttc tttatctttt ttccttttta aaatatcaga aaatcccatt atatagttca
     2521 ctatattaag ttctttttaa attatatcaa ttatacaatt cgctagcgat agatcgaaat
     2581 aattttactc cattacaatc atagttagtg tagatttaac tcctggtaat tttcttaact
     2641 tccatgtaat aatttcttta accttatcca ttgtatcaga ttctactttt gcaataatat
     2701 catagacacc atagacttga aaaacttctt taactccttc taagttttta agttctttta
     2761 ctatagaatc ttcacttccc aactcagtat ttaaaagaac aaaagcgcca ggcattatat
     2821 tttataataa aatgatatat aaaaacatgc ctttaaaaaa gtaaaaggga tatctaagtt
     2881 tcactcaatt ttttatcatc aaaaggacca gccaactccc actgataatc aaaaaaatcc
     2941 ttacaccaat catgaaaatt ttgatcccta ctatagaaca taatattcaa atcgggttca
     3001 ccttttagat ttggaaatag cagacatgct tcttgctcat tgaatattgt cattatagta
     3061 accttattaa tcatctttct ttctaccata cctttagaaa tttgattcac ccagcctatt
     3121 ttttgtaata attctcctct tcctttagga ataatcgtat ctttagaaaa aatgtatgaa
     3181 aatttaatac cggtctcaat tttattactt actgtttcaa tcaaatctaa tggaacttga
     3241 gacataattt ctctgatata gactttggag ttccagtaaa gtgtcttcca acgttgtaga
     3301 tttgccatta cacccaatac aatttcacta ttttgtaaag aaccaattct ttgaataaat
     3361 tttaatggca attcttcgat tgaatgttca ataaaatatt gtttattttg taaaagaaat
     3421 tcatatccag gaattaaaaa aacaattgct ttaccataag atgtcaatcc taaatcacct
     3481 tctccatctt tataaaccaa tccagactcg actaagcgga ttatgtttct atgtgcttcc
     3541 tgcatagtag catctaattc cgttgacaat tgagataatc tatacttgtg tttagatagc
     3601 tttaataaca tagaaagcct taaatctcca gccaattcaa agaataacga ttcggttttt
     3661 tcaggaatgt tattaggcat tttattatat tatagttctc ttcaaagatt taaacttgaa
     3721 taatttaaaa tccaaattaa tactagaaag ggtaaaagta tttaaaaaaa acaaaacatt
     3781 atggatatat tcttctaaga gttctaggga agggcaaggt atcttttatg tcaggaatgt
     3841 ttagaatcca tctcattaat ctttctattc ccaatccaaa tcccccatga ggtaccgagc
     3901 catatctccg taaatccaga taccaatcat aatcactttt ttgtaaccct tccaaaacca
     3961 ttctcttttc caatttttgg agattatctt ctcttaaacc tcctgatgtg atttcaccaa
     4021 aa
//
LOCUS       DATAFW010000091         2608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4449959, whole genome shotgun sequence.
ACCESSION   DATAFW010000091 DATAFW010000000
VERSION     DATAFW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4449959"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..644
                     /locus_tag="VHJ57_02195"
     CDS             198..644
                     /locus_tag="VHJ57_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2487378.1"
                     /translation="MIKFINKFIINEDIEKVWEFYTDIRHLEIITPKKLNLKIIKSTN
                     QKIILGQETIISAKIIIIQSTWHSKITLFQQYEYIDEMLEGPLKKWKHTHTFKRINEN
                     KTEIIDKVEFSLPYGIFGKIASIFFIKILRQLFEHRKDETIKYLSR"
     gene            complement(890..>2608)
                     /locus_tag="VHJ57_02200"
     CDS             complement(890..>2608)
                     /locus_tag="VHJ57_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEX2487379.1"
                     /translation="IDRITYEDIGGIKNEVQKVREMIELPLRHPELFDKIGIEAPKGV
                     LLHGPPGTGKTLLAKAVANETNANFYSIGGPEIMSKFYGESEEKLRDIFLQAQEKAPS
                     IIFIDEIDSIAPKRDEVSGDVEKRIVSQLLTLMDGLKTRGKVVVIAATNRQNAIDPAL
                     RRPGRFDREIEIGIPDEEGRYDILQIHTRGMPLEQDIDLKSYAKVTHGFVGADLEMVA
                     KEAAMRSLRRIIPEINLKESKIPIDVLNKIKITSQDFDDALKEVHPSAMREVLVQKPD
                     IEWKDIGGLYNVKEELKEAIEWPLKHADLFSKAAIKPPKGILLYGPAGTGKTLLAKAV
                     ASTSASNFISIKGPELLSKWVGESEKGVREIFRKARQASPCIIFFDEIDAIAPRRSGS
                     DGGGDSHVTERLVSQMLTEIDGLEDLKGVVIIGATNRPDIIDEALLRPGRFDRLLEIP
                     IPDNATRKQIFQIHLKKKPLGSDVDIDKLVGLTDSFTGAEIEALVNAAAIVAVKEHVI
                     VREQKEMNNSQKTDTNIPDSSKINEKKENKLKEELEITMKHFQSSFSKIAKKNKPSLP
                     RISSIA"
BASE COUNT          838 a          448 c          342 g          980 t
ORIGIN      
        1 catctaattc aaatagtttg gctagattac ctaaatgctc acatcctata ttttgaataa
       61 ccaaaatatt cttagacaag ataaaaataa attttctcca attcaatata aagctaatat
      121 ttaataaata ttaaatttat acacttatcg aacctaaaga caacatttga attagatcat
      181 ctctaatatt tatttgcttg attaaattta tcaataaatt tatcataaat gaggatattg
      241 aaaaagtgtg ggaattttat actgatatta gacatttaga gattattacc ccaaaaaagt
      301 taaatttgaa aataataaag agtacaaatc aaaaaattat ccttggtcaa gaaacgatta
      361 tttcagctaa aataataatt atacagagta cttggcattc aaaaataact ttatttcagc
      421 aatatgaata tatcgatgaa atgttagaag gacctttaaa aaaatggaaa catacacata
      481 catttaaaag aattaacgag aataaaacag aaattatcga taaagttgaa tttagtttac
      541 catatggaat ttttgggaaa attgcttcaa tattttttat taaaatatta agacaactct
      601 ttgagcatag aaaagatgaa accattaaat atttaagccg gtaaaacttt tcacataaat
      661 ttacatttct aaaaaaaaat tatagttata attatggcaa taattttatt cttaacgttg
      721 aaataatata tatatatata atcaaagaat agattttttt tatagaaatt attttttgta
      781 ttaatatagg ttaaatgaca tcaatcagtc tgtcaatcgt taattataag atttagtatt
      841 acagacacaa aaaaaattaa attattatta ttcgtttaga gaaggagaat tatgctatgc
      901 tactaattct aggcaatgag ggtttatttt tctttgcaat tttactaaat gagctttgaa
      961 aatgtttcat agttatttct agctcttctt ttaatttgtt ctcttttttt tcattaattt
     1021 tagatgaatc tggtatatta gtatcagttt tttgactatt attcatttct ttttgttctc
     1081 ttactatgac atgttctttt actgctacaa tagctgcagc attaactaac gcttctatct
     1141 ctgcaccagt aaagctgtct gttaaaccta ctaatttatc aatatcaaca tcagagccta
     1201 atggtttctt ttttaggtgt atctgaaaaa tttgttttcg cgtagcatta tcaggtattg
     1261 gtatttctaa aagacgatca aatcttccag gtcttaacaa agcttcgtca ataatatcag
     1321 gtctattagt tgcaccgata atcaccactc ctttgagatc ctccaatcca tctatctcag
     1381 ttaacatttg gctaaccaat ctttcagtta catgtgaatc tcctcctccg tcagatcctg
     1441 atcttcgtgg agcaatagcg tctatttcat caaaaaatat tatgcacggt gatgcttgtc
     1501 tagcttttct aaatatttct ctaactcctt tttcagattc tccaacccat tttgataaaa
     1561 gctctggacc ttttatacta ataaaattgg atgcagaggt tgatgcaacg gctttagcta
     1621 ataaagtttt tccagtcccg gcaggtccat ataataatat tcctttagga ggttttattg
     1681 ctgctttgct aaaaagatcg gcatgtttaa gtggccattc tattgcttct tttaattctt
     1741 ctttaacatt atatagacct ccaatatctt tccattctat atctggtttt tgcactaaaa
     1801 cttccctcat tgcagatgga tgaacctcct ttaaagcatc atcaaaatct tggctagtta
     1861 tttttatttt atttagtacg tctatcggaa tttttgactc ttttaaattt atttcaggta
     1921 taattcgacg taaagatctc attgcagctt ctttcgccac catttccaag tctgcaccta
     1981 cgaatccatg agtaactttg gcataagatt ttaggtcaat gtcttgttcc aagggcattc
     2041 ctctggtatg aatttgaaga atgtcataac gtccctcttc atcggggata ccaatttcta
     2101 tttctctgtc aaatctacct ggtctcctca atgcaggatc aattgcattt tgtctattcg
     2161 tagctgcaat tacaacaact ttacctcttg ttttgagtcc atccattaaa gtcaaaagct
     2221 gagatacgat tcttttctca acatcacctg acacttcatc cctttttggt gcaatagaat
     2281 caatttcatc tataaatatt atactaggag ccttttcttg tgcctgtagg aatatatctc
     2341 ttaacttttc ttcgctttct ccataaaatt tgctcattat ttcaggacca ccaatagaat
     2401 agaaattagc gtttgtctca ttagctactg cttttgcaag caaggtcttt ccagtacctg
     2461 gaggtccatg taataataca ccctttggtg cctctattcc aattttatca aaaagttctg
     2521 gatgccttaa tggaagttca atcatttctc gtactttttg tacctcattt ttgattcctc
     2581 ctatatcctc atatgtaatt cgatcaat
//
LOCUS       DATAFW010000092         5163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1455109, whole genome shotgun sequence.
ACCESSION   DATAFW010000092 DATAFW010000000
VERSION     DATAFW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455109"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..241
                     /gene="lysW/argW"
                     /locus_tag="VHJ57_02205"
     CDS             74..241
                     /gene="lysW/argW"
                     /locus_tag="VHJ57_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-aminoadipate/glutamate carrier protein
                     LysW/ArgW"
                     /protein_id="HEX2487380.1"
                     /translation="MVKCEECDAEIKIPEDSIEGEIVSCPDCGSSYELVKNEGGFTTK
                     PAQVVGEDWGQ"
     gene            238..1113
                     /gene="lysX"
                     /locus_tag="VHJ57_02210"
     CDS             238..1113
                     /gene="lysX"
                     /locus_tag="VHJ57_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEX2487381.1"
                     /translation="MTEGKNTSSSLTLLYDNIRWEEKAIYEAAKKKGIEIKKIDCKDI
                     VLDLNILSSEYTNRTIIQRCVSYFKNLHSTAALEGLGANVINPLNTNVTCGNKLFTHM
                     LLQKKGIRTPKVITAFSPDSALSALETFGYPAIIKPTIGSWGRLIALLRDKDAAKAVL
                     EDREHMFPLYHVYYLEEFVSRPPRDIRAIVVGDVVVAAIYRYSESDEWKTNMALGGRA
                     ENCPVTNELEDICIKSSRAVGGKIVGVDLMESSKDGLLVHEVNNTTEFKNTVKITEIN
                     IQNMIVDYSLQYQNN"
     gene            1121..2236
                     /locus_tag="VHJ57_02215"
     CDS             1121..2236
                     /locus_tag="VHJ57_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2487382.1"
                     /translation="MVSSNYAIELLKKSLNLYTPSRSEGPLANLIKDKCVNELGFEQV
                     HIDSVGNIIAIKGRGYPRILLCGHMDTVPNKVPVRIENGFLYGRGASDAKAPLISMLL
                     AASEFPKQNGTIVFAGVVDEEGNATGSKHLVKNNLDLDYAIFGEPSGIENITISYKGR
                     LALKLICDALDSAHASAPWLAKNSIEEAYNFWNEIKNALNKNYEKEKKSNSVSCCLTE
                     ILGGSSHNVTPQKCKITMDIRIPNGITCQQVFDSIKILISKISKENGIKTNFIIEDFT
                     EPFEADFSSPLVRSLVLSILDICKKRPALLRKTGTGDMNIIGNAFKIPVVTYGPGDPH
                     SSHSVNEHVNIEEYLSGIEVYKRALFHMSRMYNRQNK"
     gene            2272..3363
                     /gene="dph5"
                     /locus_tag="VHJ57_02220"
     CDS             2272..3363
                     /gene="dph5"
                     /locus_tag="VHJ57_02220"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HEX2487383.1"
                     /translation="MLCFVGLGINGYAGLSLLARETLKKCQFIYIERFTGYIDDDDIR
                     QLKNLLENGNKSIQIVSRWFIEDARSIISRSKDDNVAILTYGDPFMATTFNELYVRAK
                     KNLIEIKIIHGVSGMSSLIGEIGLHNYKFGRTTTIMSDPMSAISVYNIIYENLRVGNH
                     TLILTEYNNNNEKSFFFLDPARAIDMLLEVEENIRYKIISDELFIIVASRIGTKDQNI
                     LGGKVKSIKKIDFGKGPHSIIVTGHLHFTEIDSITSLYPNKDPPLDNTQFFRHVAVTM
                     LEKYLPKAKYALKNMKSYLEREKNSASILNEQDKELDHVLENAEYYLLDAERFFNQHK
                     FELGILSIGYAEGLIDALRFQKNINPWLS"
     gene            3459..4856
                     /gene="gvpD"
                     /locus_tag="VHJ57_02225"
     CDS             3459..4856
                     /gene="gvpD"
                     /locus_tag="VHJ57_02225"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpD P-loop
                     domain-containing protein"
                     /protein_id="HEX2487384.1"
                     /translation="MTKNYDISFKIPDDLLQFLNNETYSLLIKGEPGAGKTALCLTIL
                     KALNIKNNFFYISTRVSPNKLFYYFKWINKYLKIKYTNNHNKSSKHLNSLEPFFEDAR
                     LDEPESLFERITNQLMDVRSPLIIVDSWDGIASFMDRESRLNNERVLQTWLERAGGRL
                     ILVNEGNELTTLDYLVDGIVILKKNQFNHINVREMNLTKLGGIDIKKNSFVYTLNNGI
                     FKTYEPIFHYDDILNQILNLPTNKLFIQNKIIKIDSGHLSLNTDLNGGFTKKSIVLLE
                     YDPNLEMRYIILFLFNFITNCISNSHILLLDTKIAKDIPFLLNLIRFNAVDSNDYDKF
                     VKLIDLNLETNIENLNDNLTTDLLINPISTEIKNIKTNSMIFSILNFDRYKNIDVYKL
                     INALKEKLDLSFLLLQTGIRSELSPGLFDYKLTIKNFNDVICIHLTYPYLRLYGLEVV
                     NIEKSNFSIRLDPLI"
     gene            complement(5016..5089)
                     /locus_tag="VHJ57_02230"
     tRNA            complement(5016..5089)
                     /locus_tag="VHJ57_02230"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5053..5055),aa:Ile,seq:gat)
BASE COUNT         1928 a          685 c          844 g         1706 t
ORIGIN      
        1 tatcttgatg agaaattaga aagaaagata cttaatgaag aaacatttaa tgtaaaaaag
       61 gagtgaataa aatatggtaa aatgtgagga atgtgatgcc gaaatcaaaa taccagaaga
      121 ttcaatcgaa ggtgaaatcg tatcctgtcc agattgtgga tcaagctatg aattggtaaa
      181 aaatgaaggt ggatttacta caaaacccgc tcaagtagtc ggcgaggatt ggggacagtg
      241 actgagggaa agaatacatc ttcttctctc acacttcttt atgataatat aagatgggaa
      301 gaaaaggcta tctatgaagc tgctaaaaag aaaggaattg aaataaaaaa aattgattgc
      361 aaagatattg ttttagattt aaatatactc tcttctgaat atactaatcg aaccattatt
      421 caaagatgtg ttagttattt caagaacctc cattctactg cagcgttaga aggattagga
      481 gcaaatgtaa ttaatccgtt aaacactaat gttacttgtg gaaacaaatt atttactcat
      541 atgttactac aaaagaaagg tatacgaacc cccaaagtaa taactgcttt ttcacccgat
      601 tctgcacttt cagcattaga aacttttgga tatcctgcta ttattaagcc taccatagga
      661 agctggggta gattgatagc attattacga gataaagatg cagcaaaagc tgtcttggaa
      721 gatagagagc atatgtttcc tctttaccat gtatattatc tagaagaatt tgtttctaga
      781 ccacctcgtg atataagagc tatagtagta ggtgatgttg ttgttgctgc aatatataga
      841 tactctgaat ctgatgaatg gaaaactaat atggcattag gaggtagagc tgaaaactgt
      901 cctgttacaa atgaactgga agatatatgt attaaatctt ctcgagctgt cggtggaaag
      961 attgtaggag tagatttaat ggaaagttca aaagacggcc tattagtaca tgaagttaat
     1021 aatacaacag agttcaaaaa tacagtaaaa attactgaaa taaatattca aaatatgatc
     1081 gtagattact ctttacaata ccaaaataat tagataaaca ttggtctcat ctaattatgc
     1141 aatagaactc ttaaaaaagt cacttaatct ttatacacca tctagatctg aaggtccatt
     1201 agctaatcta ataaaggata agtgtgttaa tgaacttggg tttgaacaag ttcatataga
     1261 tagcgttgga aatattattg ccatcaaagg aagagggtat ccaagaatat tgctttgtgg
     1321 tcatatggat actgttccaa ataaagttcc tgtaaggata gaaaatggtt tcctgtatgg
     1381 tagaggtgca tcagatgcta aagctccatt aatctctatg cttttggccg cctctgagtt
     1441 ccctaaacaa aatggtacta tagtatttgc tggtgtagtt gatgaggaag gaaatgcaac
     1501 aggtagtaag catcttgtta agaataattt ggatttagat tacgccatat ttggtgaacc
     1561 aagtggtata gaaaatatta caatttccta taagggacgg ttagctctta aattaatatg
     1621 tgatgcttta gatagtgcac atgcaagtgc tccttggtta gcaaaaaact ctattgaaga
     1681 agcctataat ttttggaatg agataaaaaa tgctttaaat aagaattatg agaaagaaaa
     1741 aaaaagtaac tctgttagtt gttgtcttac agaaattttg ggtggatcta gtcacaatgt
     1801 cactcctcaa aagtgtaaaa taacaatgga tattagaatt cccaatggga tcacctgtca
     1861 acaagttttt gactctataa aaattctcat ttcaaaaata tcaaaagaaa atggaataaa
     1921 aactaatttt ataattgaag atttcacaga accattcgaa gcggattttt cctctccatt
     1981 agttagatct ctcgttcttt ctattcttga catttgtaaa aaaagacctg cattactaag
     2041 aaaaacagga actggtgaca tgaatattat cggaaatgca tttaaaattc ctgtagttac
     2101 ttacggtcct ggagatcctc attcttctca tagtgttaat gaacatgtaa atatagaaga
     2161 atacctttcc ggaattgaag tctacaaaag agccctattt catatgtcaa ggatgtataa
     2221 tagacagaat aaataatatt ggagataata atagcgcttt aaatacataa tatgttatgt
     2281 tttgttggac ttggaataaa tggttacgct ggattaagtc tgttagcaag agagacttta
     2341 aaaaaatgtc aatttatcta tatagaaaga tttactggtt atattgatga tgatgatatc
     2401 aggcaattaa aaaatttact agaaaatggt aataaaagta tacaaatagt ttcgcgttgg
     2461 tttatcgaag atgcgagatc tatcatctct agatcaaaag atgataatgt agccatattg
     2521 acttatggcg atccttttat ggcaaccacc ttcaatgaac tttatgtaag agcgaaaaaa
     2581 aacttaattg aaataaaaat aattcatggt gtttctggaa tgtcatcttt gatcggtgaa
     2641 attggattgc ataactacaa atttggaagg acgacaacaa taatgtctga tcctatgtct
     2701 gcaataagtg tctataatat tatttacgaa aatctaagag taggaaatca tactttaatc
     2761 ctaacagaat acaataataa caatgaaaag agctttttct ttctcgaccc agcaagggct
     2821 atagatatgc ttcttgaagt cgaggaaaat atccgttata aaatcatatc tgacgagtta
     2881 tttattatag tagcatctag aataggaact aaagatcaaa atatcctagg aggaaaagtt
     2941 aaatccataa aaaaaataga ttttggtaaa ggacctcatt ctatcatagt aacaggtcat
     3001 cttcatttta cagaaattga ttctataaca tctctttatc caaataaaga tcctccattg
     3061 gataatactc aattctttcg acatgttgca gtaacaatgt tagaaaaata tttaccaaaa
     3121 gctaaatatg ctttgaaaaa tatgaaatcg tatcttgaaa gagaaaaaaa ttctgcttct
     3181 atactcaatg aacaggataa agaactggat catgtattag agaatgctga atattattta
     3241 ttggatgccg aaagattttt caatcagcat aaatttgaat taggaatact tagtataggt
     3301 tatgcagaag gcttaataga tgcattacgt ttccaaaaaa atatcaaccc gtggttatct
     3361 taatttaata cctcattatt ttaaggatat tttttatatt ttgatatgga gaactattat
     3421 tataacagaa tatttattaa cttaagtgag gttgggcaat gactaaaaat tatgatattt
     3481 cttttaaaat tcctgatgat ctgttacaat ttttaaataa tgaaacttat tccttattga
     3541 ttaaaggaga accaggagca ggaaaaactg cactatgttt gacaattctt aaagcattaa
     3601 atataaaaaa taatttcttt tatatttcta caagagtatc gcctaataaa ttattttatt
     3661 actttaagtg gattaataaa tatcttaaaa ttaaatatac aaataatcat aataaatctt
     3721 ctaaacatct aaatagttta gaaccttttt ttgaagatgc acgtttagat gaaccagaat
     3781 ctcttttcga acgtataaca aatcaattga tggatgtaag atctcctctc ataatagtag
     3841 atagttggga tgggatagct tcattcatgg atcgtgaatc gagactaaat aatgaacgtg
     3901 ttttacaaac atggttagaa agagcaggtg gtagattaat tttagtaaat gagggaaacg
     3961 aattaacaac gttggattat cttgttgatg ggattgtaat tcttaaaaaa aatcaattta
     4021 atcatatcaa tgtaagagag atgaatttaa caaagttagg aggtatagat ataaagaaaa
     4081 attcatttgt atatacctta aataatggaa tttttaaaac gtatgaacca atttttcact
     4141 atgatgatat cttaaatcag attcttaatt taccaaccaa taaattattt atccaaaaca
     4201 aaattattaa aattgattct ggtcatcttt cacttaacac agatctaaat ggtggattta
     4261 ctaagaaatc tatagtacta ttagaatatg atccaaacct agaaatgaga tacattattt
     4321 tgtttttgtt taatttcatt actaattgta ttagtaattc ccatattcta ttattggaca
     4381 ctaaaattgc caaagatatt ccttttcttt taaatttgat ccgatttaat gccgtagatt
     4441 ctaatgatta cgacaaattc gttaagttaa tagatttaaa tttggaaaca aatattgaga
     4501 atttaaatga taatttaacc actgatttat taattaatcc tatttctact gaaataaaga
     4561 atattaaaac caattcgatg atatttagta ttttaaattt tgatagatat aaaaatatag
     4621 atgtgtataa attgattaat gccttgaaag aaaagttaga tctttctttt ctactacttc
     4681 aaacaggaat taggtctgaa ttatctccag ggttatttga ttataagctt acaatcaaga
     4741 attttaatga tgtaatatgt atccatttaa catatccata tttaagacta tatggccttg
     4801 aagtagtgaa tatagaaaag tctaatttct caatcagatt agatccttta atataaaata
     4861 attaacaata tcttacaatt actataatat tatatagaat ttaaacatta actgaaaaat
     4921 taattcaatt aaaagtgaat cttgaagtga attataatat ttaagtttca tcatcgctat
     4981 gtatcagtgt gattataggt atatcaataa aatcatgggc cgaaagggag ttgaaccctc
     5041 gacctttcga ttatcagtcg aacgctccac caagctgagc tatcggccca atatattaca
     5101 atctggtgtt ggtgctttat ttctaagaca atttaaatat ttttttttga acctcaatct
     5161 att
//
LOCUS       DATAFW010000093         6394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_397769, whole genome shotgun sequence.
ACCESSION   DATAFW010000093 DATAFW010000000
VERSION     DATAFW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397769"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..415)
                     /locus_tag="VHJ57_02235"
     CDS             complement(53..415)
                     /locus_tag="VHJ57_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX2487385.1"
                     /translation="MESDIKSESENISFDDFSKIVLKLGKIKKIEYLPKLNKVYKAEI
                     DLGSEERIVVVVGGAMYYKPEELEEKLVIVCTNLSPRTIGNIESKGMILALEGMEGKP
                     IFLTIDKGEKVELGSKVR"
     gene            1232..1879
                     /locus_tag="VHJ57_02240"
     CDS             1232..1879
                     /locus_tag="VHJ57_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487386.1"
                     /translation="MSLEEKIFDKIFSSGLSGVKKTDLTKEFSSVNLDNILNTWIKQN
                     KIIITKKGSTNYCWHKEGYFQYLLDSDPKFKYTFEILKEIQISFQKLSTSFDKHVEKI
                     EGKMIVIMDSILATTTNNNAFENSISNPQKVINLQNFSEDFDLTICKYSDSIGWVPLA
                     TLRNDLSHKYEISEKEFYDLAEQLVNEDRSKYELSTGGNEGIMIRGLVYGYVRCL"
     gene            1880..3490
                     /locus_tag="VHJ57_02245"
     CDS             1880..3490
                     /locus_tag="VHJ57_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487387.1"
                     /translation="MYPYNLDKNPFPSNPTPNLEDAHILGGKRHQESKNSILSCIEDL
                     VNLSETSDASEKFRIITVIQDVGSGKTHLTLNIRISGIKEKAIISYLDMSQIYPKNIP
                     SIYQAMISGFEPEYLYTLKKELLYYLKDRIKSDNKIAKKIFKLGFFESLSGKGIDNKI
                     LQILENRSEPNLRYIDNILDEDFSKNDISIIKTIIENQFENNESITLMDMINRLSTLS
                     KINLKLFNKISLYQFDEFNNNKETLSFMKAIINAHIPNTVLMLILTPSSYLKIASDDS
                     SVYDRLEKANYKIDLAGSNSFEEISDIVLEYINFYNLPTTITNDQRKELFSQIKILYE
                     DFDFRNIRSILNIMYSAFETASHKKMSIISEQILDETLRKNYPGLKIKGSLVDISISD
                     YIKIKNHIDTTSDVKKEIVNSLRELINYLHRHNIKCKYEDYLPLEEEAVNIIYKDNSD
                     SKSQITMLLNESTLTNMRSTVFNIGTTVKDVNYPKVTTTKNDSVSINLDRSKIIDILY
                     IYDKINRNLHTMADTNKIFLLAKSINLY"
     gene            3558..4019
                     /locus_tag="VHJ57_02250"
     CDS             3558..4019
                     /locus_tag="VHJ57_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487388.1"
                     /translation="MKFRQFTTLNDLISNIHEQGFGVIEYANNKVACIYVQQPLYDTI
                     MEKCIGKKYVVDVLLNIFYDGHYVFVDIQLEFIDLGIEENFLVPGNTSMNFFDALNES
                     GLLGIIPKSNGHISSSNIFLIQLPKKERISKAVDIIKSNLKQSNIKDEKTT"
     gene            4003..4224
                     /locus_tag="VHJ57_02255"
     CDS             4003..4224
                     /locus_tag="VHJ57_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487389.1"
                     /translation="MKRQPKTKKKSYEDSDLHPTKDTVTSSPIPKEIDTHYKLFGKHI
                     WEIKFFEKCEICNSPFDEFGFCACGSGKS"
     gene            complement(4247..4321)
                     /locus_tag="VHJ57_02260"
     tRNA            complement(4247..4321)
                     /locus_tag="VHJ57_02260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4283..4285),aa:Gly,seq:gcc)
     gene            complement(4927..5550)
                     /locus_tag="VHJ57_02265"
     CDS             complement(4927..5550)
                     /locus_tag="VHJ57_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487390.1"
                     /translation="MSIKNINTEKESIKNYQYTCSEFIKDLSLDYLNWVDRYNLPPEE
                     SKIRKQKINNIVIKSSYIIYKFGDTIKKIDIIMNHGISKMAEYTAGYPFQFSILVNNL
                     TSYTYSPRGEIRLNIKAIPNFGKEVRIGSYDKDNLILLNSSSPVTYSFNKSSLESADI
                     LDDYIIIDAKNCLKNEILSATVIVEEIKDNIVFEKRGFSTLILVTQT"
     gene            5614..6084
                     /locus_tag="VHJ57_02270"
     CDS             5614..6084
                     /locus_tag="VHJ57_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487391.1"
                     /translation="MMFSKIIEDLKMNFNTNRDFFNKYLKSNPNMIYSKINEITQFVG
                     ARYGIDLKLHFPDPKKIYIFDSYGSENIGIVVDRFSKKFSISRELVKSKAIETLPGAT
                     VSDAYMYEGKEGVRISFKKGRLELLPGSIHIWCSIDKDVENYVNWILTNVLVKH"
     gene            complement(6102..>6394)
                     /locus_tag="VHJ57_02275"
     CDS             complement(6102..>6394)
                     /locus_tag="VHJ57_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487392.1"
                     /translation="QIKDITREPKYLAIGAKWDSINTGITTIPIKITSKEYLFSKVGK
                     LETFVLSYKIKDRSSNMWISKNLPLPVKAEIYNEVDKLKYKYDIIRLNRDIK"
BASE COUNT         2365 a          851 c          889 g         2289 t
ORIGIN      
        1 cacaagtatt agagttctta ttattagtat taactatgta atattataaa aactaacgaa
       61 ctttagaacc taattcaact ttctcccctt tatctattgt taaaaaaatt ggttttccct
      121 ccattccctc taatgctaaa atcattcctt ttgattctat atttccaatt gtccgaggtg
      181 aaagatttgt acatacaatt actaactttt cttccaattc ttcaggtttg taatacatgg
      241 cacctccaac tactactact attctttcct cagagccgag atctatctct gctttgtaaa
      301 ctttgttcaa cttcggtaaa tattcaattt tttttatctt tccaagtttt aagacaattt
      361 tactaaaatc gtcaaaggag atattctcag attcactttt aatatcgctt tccattttaa
      421 ctatttagac atataaattt ctgttatata atatatcatt aatataaatt attgtacttt
      481 ttggtcatat atactaaggc ttcagttgaa ttttttaagt ccgatcaata atttattcaa
      541 tatttggaaa aggtaacaac aacaaatatt tggatagctg ctaaaccttg gtgacaaact
      601 gataatacaa tgaacaagta aacaaactaa gaatatcttt aatctaattc aacacgataa
      661 tacccaattc acaacgatag actattaaaa ctagtaaaag tatctgataa agttctagac
      721 acaaaattaa acggtattta taataataat aataagaatt tctgcaaata agggatataa
      781 aaaatgcttg taagtttata tagcaaaggc aaagaaaagg gatggtatta tagaatagat
      841 gaaaccttta ttcaatcgat taccaccaat gtgatgaatg cttattttaa ccatgtctcc
      901 taggccgttc ttcctgcctg tatttgcaat ctatatgcta cataagatag aaaatatatg
      961 cttgttgtaa aaatgatctt acattgctgt ttgaaaataa caaaccatag gtatagagga
     1021 caatgtagca tgttatgatt tcaacgaaaa tatatgaaca atacaaacta tattcggtca
     1081 gataccctag tgctttcatc atgtttcaga ataggatttc atcattccat ttatctttat
     1141 ttgattctat attaaaagtt aaactaaatt agctaattat atagtataaa catacaaagg
     1201 atcttttata cgaaaatcaa acttgtagtc tatgtcttta gaagaaaaaa tatttgacaa
     1261 aattttttct tcaggtttat ctggagtgaa aaaaactgat ctaactaaag aattttcatc
     1321 agtaaatctg gataatatct tgaacacatg gataaaacaa aacaaaatta ttataacaaa
     1381 aaaggggagt acaaattatt gttggcataa agaaggttat tttcaatatc tcttggattc
     1441 cgatccaaaa ttcaaatata cttttgaaat actaaaagaa attcaaattt cttttcaaaa
     1501 attatccact tcttttgata aacatgtaga aaaaatagaa ggtaagatga ttgtcataat
     1561 ggattcaatc ttggcaacaa ctaccaataa taatgccttc gaaaatagta tttctaatcc
     1621 acaaaaggta attaatttac aaaacttcag tgaagatttt gatttaacta tatgtaaata
     1681 ctctgattca atcggatggg ttcctttagc cactcttaga aacgatctct ctcataaata
     1741 tgagatttct gaaaaggaat tctatgacct tgctgaacaa ttagttaatg aagatagatc
     1801 aaaatatgaa ctatctacag gtgggaatga gggaattatg attcgtggcc tggtatatgg
     1861 ttatgtaagg tgcttgtaat tgtatccata taaccttgac aaaaatcctt ttccaagtaa
     1921 tcctacacca aatttagagg atgctcacat tttaggtgga aaaaggcatc aagagtctaa
     1981 aaattcaatt ttgtcatgta ttgaagatct tgtaaaccta tcggaaacaa gtgacgctag
     2041 tgaaaaattc agaataatta ctgttattca agatgtaggt tcaggaaaaa cacatctcac
     2101 acttaatata agaatttcag gaataaagga aaaagcaatc atttcttatc ttgacatgtc
     2161 acaaatatac ccaaaaaata ttccaagtat ttatcaagct atgattagtg ggtttgaacc
     2221 tgagtacttg tatactttga aaaaagaatt actttattat ctaaaagatc gaattaaatc
     2281 agataacaaa attgcaaaaa aaatatttaa attaggattt tttgaatcgt tatcaggtaa
     2341 aggtattgat aataaaattc ttcagatatt agaaaataga tcggagccta atttaagata
     2401 cattgataat attcttgatg aggatttttc gaaaaatgac atatctataa ttaaaactat
     2461 catagagaat caatttgaaa ataatgaatc aatcacttta atggatatga taaatcgcct
     2521 ttcaacttta tctaaaatta atttaaaatt atttaataaa atttctttgt atcaattcga
     2581 cgaatttaat aataataaag aaacgctttc atttatgaag gctattatta atgctcatat
     2641 acccaatacg gtcttaatgc taattctcac tccttcctca tatctaaaga tagcctctga
     2701 tgatagttca gtttatgata ggctggagaa agctaattat aaaattgatc tcgctggatc
     2761 aaattctttc gaggaaatat ccgatatagt attagaatat attaattttt ataatctacc
     2821 tactactata acaaatgatc aaagaaagga acttttctca caaataaaaa tattatatga
     2881 agattttgat tttagaaata taagatctat tttgaatata atgtatagtg cgtttgaaac
     2941 agcaagtcat aaaaaaatgt ccataataag tgaacaaatt ttggatgaaa cattacgtaa
     3001 aaactatcca ggtttaaaaa taaagggaag cctagtagat atatcaattt ctgactatat
     3061 taaaattaaa aatcatatcg acaccactag tgatgtaaag aaagaaattg taaactcatt
     3121 gagagaatta ataaattatc ttcaccgaca taatataaaa tgtaaatatg aagattattt
     3181 acctttagaa gaagaagccg tcaacataat ctacaaggat aatagtgatt caaaatctca
     3241 aatcacgatg cttttaaatg aatcaacttt aactaacatg agaagtaccg tctttaatat
     3301 tggaacaaca gtgaaggatg ttaactatcc aaaggttaca actactaaaa acgattctgt
     3361 atctattaat ttagatcgat caaaaataat tgatatatta tatatttatg ataaaattaa
     3421 tcgtaatctg catactatgg ctgataccaa taaaatattt ttattagcaa aatcgatcaa
     3481 cctctattaa attttttttg tttgtaaata aaactattaa gaatagacta aaatatctat
     3541 atattgtatt atagatagtg aaatttagac aatttactac tcttaacgac ttaattagca
     3601 atattcatga acagggattt ggagtaattg aatatgctaa caataaagtt gcttgtatat
     3661 atgttcaaca acctttgtat gatactatta tggagaaatg tatagggaaa aaatatgtag
     3721 ttgatgtttt actaaatatt ttttatgatg gtcattatgt atttgtagat attcagctgg
     3781 aatttataga cttaggaatt gaagaaaatt tcctagttcc gggcaatact tcgatgaact
     3841 tttttgatgc attaaatgaa agtggattgc taggaattat tccaaaaagt aatggacata
     3901 tctcttcatc caatattttt ctcattcaac ttcccaaaaa agaacgcata tccaaagcgg
     3961 tagatataat caagtccaat ttaaaacagt caaatattaa agatgaaaag acaacctaaa
     4021 accaaaaaaa aatcttacga agatagtgat ttacatccta ctaaagatac tgttacatct
     4081 tcacctattc cgaaggaaat tgatactcat tataaattat ttggaaaaca catctgggag
     4141 attaaattct tcgaaaaatg tgaaatctgt aattctcctt ttgatgaatt tggtttctgt
     4201 gcctgtggga gtggtaagag ttaacagaaa cattctctat aaagaatgcg acgaccggga
     4261 tttgaacccg ggttaccaga ttggcaatct gatgtcctag accaaactgg acgatcgtcg
     4321 cccatacgat ttcagttatg ttggttattt agcctttttt atttggaaag gaactgattt
     4381 gagctggact attgagtata tcgagtctta gagatggaaa tttgtaggtg gattattttt
     4441 tagatgagag aggtttataa tgatcatatt ctatcatgat cttttgtgtt tttttgatat
     4501 ggatttgaat ttttatacaa gataaatctc aaacttttcc ttttgtgtga gtagtcagag
     4561 ttatttttct ttatgtaaga taattaatac atttaattta attcttatta ttaaatattg
     4621 tttcagaata tattaaatat atgttccttt ttaaatatta ttataagcat atatatgaaa
     4681 aaatgttcct tattgttgat atttatattt taatctttaa attaattaaa tttagattat
     4741 tttaatactg ataccaaata aatcttagtc tctttcatag aaacaatatt ggtttatact
     4801 tataataata tgtacttatt aggggaagtg tcttaataag atctgatcga cttctgaaag
     4861 tagaattcaa taatataatg aaagagattt aaattaaatt atcagtctga atcgtgattt
     4921 catagcttag gtttgggtaa ctaaaatcaa tgttgaaaag cctctctttt caaagacaat
     4981 gttgtcttta atctcttcca caattacagt agcggaaagg atttcatttt taagacaatt
     5041 tttggcatca attattatgt aatcatctaa tatatctgca gattctaggc ttgatttgtt
     5101 aaacgaataa gtaacaggtg atgaagagtt taataaaatt aaattatcct tgtcgtaact
     5161 acctattcta acttcttttc caaaatttgg aatggccttt atatttaatc ttatttcacc
     5221 tctaggactg tatgtatagc ttgttaaatt atttacaaga atcgaaaatt gaaaaggata
     5281 gcctgctgta tattctgcca ttttagagat gccatgattc ataatgatat ctattttttt
     5341 tattgtgtcc ccaaacttat agataatata actcgatttg ataacaatat tattaatttt
     5401 ttgttttcta attttcgatt cttccggagg aagattgtat ctatcgaccc aattaagata
     5461 gtctaatgat aaatctttaa taaattctga acatgtatac tgataatttt ttatactttc
     5521 tttttcggta ttgatatttt ttatagacat aatttagtta tatatctaaa aagtataaga
     5581 gaatataaat agatatagca tgatgtgata tttatgatgt ttagcaaaat tattgaagat
     5641 ttaaaaatga actttaatac aaatagagat ttctttaata aatatttaaa aagcaatcca
     5701 aatatgattt attcaaaaat aaacgaaata actcaatttg taggtgcacg atatggaata
     5761 gatttaaagt tgcattttcc agatcctaaa aaaatctata tattcgattc ttatggaagt
     5821 gaaaatattg gaattgtcgt tgatcgattt agtaaaaaat tttccatatc acgtgaatta
     5881 gtaaaatcta aagcaataga aactttacca ggtgcaacag tttcagatgc ttatatgtat
     5941 gaaggaaagg aaggagttcg catatcattt aagaagggaa gactagaatt attaccaggg
     6001 tcaatacata tttggtgtag tattgataaa gatgttgaaa attatgttaa ttggatattg
     6061 acaaatgtat tagttaaaca ttagaataaa tttttccatt cttatttaat atctctattt
     6121 aaccttatta tatcatattt atattttaat ttgtccacct cattgtaaat ttcagctttt
     6181 acaggcaatg gcaagttctt tgaaatccac atattgctgc tacgatcttt tattttataa
     6241 ctcaaaacaa atgtttccaa ttttccaact tttgagaaca aatattcttt tgaagtaatt
     6301 ttgataggta tagttgttat tccagtatta atcgaatccc attttgctcc aattgctaaa
     6361 tattttggtt ctcttgtgat atcttttatt tgta
//
LOCUS       DATAFW010000094         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_925764, whole genome shotgun sequence.
ACCESSION   DATAFW010000094 DATAFW010000000
VERSION     DATAFW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925764"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VHJ57_02280"
     CDS             <1..904
                     /locus_tag="VHJ57_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2487393.1"
                     /translation="YPLQQVDDFYLQLSKYTKIDKKYLAIGNGSDQIIDLILSVFGKE
                     KRVTTFTPTFSYFIDRCLLYSIKIDKIPLLQDNSIDQVKFIQSAKKSEIIYICSPNNP
                     TGNQFDKELLIDLIKNFDDKLIIIDEAYVEFASYSFCSETIKSQNIILLRTLSKAFGL
                     AGARLGYCIANEDFSHIFKTIIQYPYAINNISLNIGSEILRNSNYIEKTIKVIKKERK
                     RILDSLKEINNEIKVFQTESNFIFFQILDINKYEHILTQLKKDNIAVKTIGRIKDRKG
                     GHIRVTIGTKMMNNKFLRSLNKVI"
     gene            904..1944
                     /locus_tag="VHJ57_02285"
     CDS             904..1944
                     /locus_tag="VHJ57_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX2487394.1"
                     /translation="MYQKINNNNFIPTMRRCLHIDYIIFDIDGVLIDVKKSYNEAIKN
                     TVQFIVRNLIKRDLKDLVTDKIILKFRQTGGFNNDADTSYAISLALLSHQDLVENANL
                     EKFLIEVAEHADETGIESVEKYLKKLKLNKISPSDYLINIDKILDYLNYPGKVGDSIV
                     STVFDEFFYGQELFLERYKIKSKYYFGKPLIENDRIVITDNTVRKLIERFNDKIAMVS
                     GRSKIAAAYALDNKFYLLNEKNSIFLEDEDRKFAKPNPYGLKKIINKINTRNDILYCG
                     DSVEDLIMARRAEEELNQKNANKAKTNIVFCGIYGCSSNTDELISKFMEKKADIIIKS
                     VNDLPYILNKVS"
     gene            1958..>2500
                     /locus_tag="VHJ57_02290"
     CDS             1958..>2500
                     /locus_tag="VHJ57_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HEX2487395.1"
                     /translation="MNSEPLDLDKSQKRISVKDRCTKETDVSVSVNIDGSGNVEIKTG
                     IPFIDHLISAFGKHSMIDIYLEAKSNDSISHHLIEDVAITLSQTLNDALSNRTQIRRY
                     GYAIIPMDEVMAISAIDLIKRQFHKVNLGLTRKEIEGIAREDLEHFIESLLQNLNACT
                     HINIQYGENDHHKIEAAIKSF"
BASE COUNT         1042 a          267 c          388 g          803 t
ORIGIN      
        1 atatccatta caacaagttg atgactttta tttacagctt tcaaaatata ctaaaataga
       61 taaaaaatat ttagctattg gaaatggctc tgatcaaata atagatttaa tcctgtcagt
      121 ctttggaaaa gaaaaaagag ttactacctt tactcctact ttttcttatt ttattgatag
      181 atgtttatta tattccataa aaatagacaa aatacctctt ttacaagata attctattga
      241 tcaagtaaag tttattcaaa gtgcaaaaaa atctgaaata atatacatct gttcgccaaa
      301 taatcctacc ggtaatcaat ttgataagga actattaata gatttaatta agaattttga
      361 tgataaatta attattatag atgaagcgta tgtagagttt gctagttact cattttgttc
      421 tgaaacaata aaaagtcaaa atataatact attaagaact ttatcaaaag catttggatt
      481 agcaggagca agattgggct attgtattgc taatgaagat ttttcgcata tatttaaaac
      541 tatcatacag tatccttatg caataaataa tatttctttg aatataggaa gtgaaatatt
      601 aagaaattca aattatattg agaaaactat taaagttata aagaaagaac gtaaaagaat
      661 attagattct ttaaaagaaa ttaataatga aataaaagtt tttcaaacgg agtctaattt
      721 catttttttt caaatacttg atattaataa atatgaacat attttaaccc agttaaaaaa
      781 agataatatt gcagttaaaa caataggaag gataaaagat agaaaaggag gtcatattag
      841 ggtaactatc ggaacaaaaa tgatgaataa taaattttta agatccttaa ataaggtaat
      901 ataatgtatc aaaaaataaa taataataat tttattccta caatgagaag atgtttacat
      961 attgattata ttatttttga tattgatgga gttttaattg acgtaaaaaa atcttataat
     1021 gaagcaataa aaaatacagt tcaatttata gtcagaaatc tgattaaaag agatttaaaa
     1081 gatttagtta cagataagat cattttgaaa tttagacaga ctggaggttt caacaatgat
     1141 gctgatacaa gctatgcaat tagtttagca ctattatcac atcaagacct tgttgaaaat
     1201 gctaatcttg aaaaatttct aattgaggta gcagagcatg ctgatgaaac tgggatagaa
     1261 tcggttgaga aataccttaa aaaattaaaa ttgaataaaa tctctccttc tgattatttg
     1321 ataaatattg ataaaattct agattatttg aactatcctg gaaaagttgg agacagtata
     1381 gtttctacgg tatttgacga atttttttat ggacaagaat tatttttaga aagatataaa
     1441 attaaatcaa aatattattt tggcaaaccg cttatagaaa atgatagaat agtgattaca
     1501 gataatacag ttagaaaatt aatagagaga tttaacgata aaattgcaat ggtttcagga
     1561 agaagtaaga ttgcagcagc ctacgccctc gataataaat tttatttgtt aaacgagaag
     1621 aatagcatat ttctagagga tgaggacaga aaattcgcta aaccaaatcc atatggatta
     1681 aaaaaaataa taaataaaat taacacaaga aatgatattc tctattgtgg agactctgtt
     1741 gaagatctaa taatggctag aagagcagaa gaagaattaa atcaaaaaaa cgctaacaaa
     1801 gctaaaacta atatagtttt ttgtggaatc tatggttgta gtagtaacac agacgagtta
     1861 attagtaagt ttatggagaa aaaagcagat ataataatta aaagtgtaaa tgatttacca
     1921 tatatattaa ataaagtatc ataaggaata ttgttagttg aattcggaac ctcttgactt
     1981 ggataaatct cagaaaagaa tatcagtcaa agatagatgt acaaaagaga ccgatgtaag
     2041 tgtgagtgtt aatattgacg gctctgggaa tgtggaaatt aaaactggaa ttccatttat
     2101 agatcattta atctcagcat ttggaaagca tagtatgata gatatttatt tagaagcaaa
     2161 atcaaatgat agtatttctc atcatttaat tgaagatgtg gctataactc tctctcagac
     2221 tctaaatgat gcgttatcaa atagaacaca aattagaaga tatggatatg caataattcc
     2281 aatggatgaa gtaatggcaa tttcagcaat tgatctaatc aaaaggcaat tccataaagt
     2341 aaatttagga ttaacaagaa aggaaattga aggtattgca agagaagatt tggaacattt
     2401 tatagaaagt ttattacaaa atcttaatgc atgtactcat attaatatac aatatggcga
     2461 aaatgaccac cataaaatag aggcagcaat aaagtcattt
//
LOCUS       DATAFW010000095         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5862673, whole genome shotgun sequence.
ACCESSION   DATAFW010000095 DATAFW010000000
VERSION     DATAFW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5862673"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VHJ57_02295"
     CDS             complement(<1..536)
                     /locus_tag="VHJ57_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487396.1"
                     /translation="MKLLYFKIIPPFTISNQLGNFKKSFSLYYYDLVRTLFVTLAFTG
                     ILSTGWFGIPLAPKYFTDMPINNLDIIFAIFSSLGSIWGIMAYREFTKTRDNILLVRD
                     LCKKIDPLIGDYNYSTFNPITKKYESGYFPTGPTDFWDIETVVPEWLDKGKYWHIMLR
                     LQIKNKEIILKVVKDNDLP"
     gene            complement(657..986)
                     /locus_tag="VHJ57_02300"
     CDS             complement(657..986)
                     /locus_tag="VHJ57_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487397.1"
                     /translation="MYSLTEIKNSCDIDKNETICFLNNILENDTYKIILSVIENSKTV
                     YQIHKEISLPLSSIYKRIKKLEDLGILSIDKITLNNKGRKLIFYKSKIKNLTFKLNEK
                     DVSLLIT"
     gene            complement(1047..3479)
                     /locus_tag="VHJ57_02305"
     CDS             complement(1047..3479)
                     /locus_tag="VHJ57_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX2487398.1"
                     /translation="MVNKINSKINTIKLYYVFSFIVFFLITLLFLNNGYSQSDSVSED
                     LDSPLDIKKKFIIYINLERINSQLDLSEKNIASNNLDKAFSHAYIPHTNIFPNIKSDL
                     QFAAPELSSQLEKLLTDLPIFLKDVKTKPIEEIKQTIDEIRNTNYLLNSQIVGNNTLS
                     DKNFLIQSSIQLLQDASNLISITNSSQNQEQKNLQYDNIKGILERSQYQFNQIPSNEF
                     NGNEMNQVILLYSKIQQNLNQNNFNEVSSSITSLQNYLKELLNTNNNSQNLPYEYQNY
                     FSTIDTLLSNVLVEIRENNDYRQAEKHAMSAYLDNYEYLEAPIEKNDPNLMVDIEIDM
                     REDLRKLIKNNEKLEVIEPFITQIKTKLDNAKLILENDSSIVNNNNNNNNTTELISSR
                     NNLANIEELSKGFGSFQGDRREMGESTNPQKQGVRNDIDQIRLNLQKVLNLYKEEKYD
                     EALVVARSAYLDSYENIELPLRPIDPDFTLDMEIKFAELRNMIEQKSEYENIVKKIGE
                     IRKGLDESERLVSGIGTIAPGIAFSSSFSIIFREGLESALIIGAIITYLEASRNERFK
                     KHVYYGIILAAGATALTWMIAEFFIELSGASRELIEAIAGISAVAVLFWVSFWVLNKI
                     ETKKWIEFVKSKVWKATTTGSVMVFVLLSFFTVFREGFETVLFYQAMLSYAKHMELFV
                     ILGMIIGLAVIIGVALIVRKLGKKLPLRVLFGLTMGIGAYMSITFLGNAIREFQEVGY
                     ITTTHLFGIIPRLDVNLATMTGIHPTLETVVAQLILLSVYLVGSAYILVIQPRKKKLI
                     ESSRKSMSDFEK"
     gene            complement(3836..>4293)
                     /locus_tag="VHJ57_02310"
     CDS             complement(3836..>4293)
                     /locus_tag="VHJ57_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487399.1"
                     /translation="ETLPKDFSNRLNKYVQTVKSDDLDKIIIEKMELEKFLNNALDKT
                     ISLEKRTNRDHILNVTADLITIASEEYTESMSQGKIINFLEYQDGQQFFKRALILLNT
                     SQTYLSDNDKNYLTEVKELQKGMNSKIDPSEIDMNVKDILCEIKQSFYC"
BASE COUNT         1420 a          615 c          581 g         1677 t
ORIGIN      
        1 ggaagatcat tatctttaac aactttaaga atgatttctt tgtttttaat ttgcagtcta
       61 agcataatat gccaatattt tcctttatct agccattcag gtaccacagt ttctatgtcc
      121 caaaaatctg tgggtcctgt cggaaagtaa cctgattcat attttttagt gatagggtta
      181 aaagttgaat agttataatc gccaataagt ggatctattt ttttgcagag atctctaact
      241 aacaaaatat tatctctggt ttttgtaaat tctctgtatg ccattatacc ccaaatactt
      301 cctaaagaac taaaaattgc aaatattata tctaaattat ttattggcat atcagtaaaa
      361 tacttgggag ctaacggaat tccgaaccaa cctgtagaga gtattcctgt gaaagcaagt
      421 gtaacaaata acgtacgaac taggtcgtaa taatagaggc taaatgattt tttaaaattg
      481 cctaattgat tagaaattgt aaaagggggg ataatcttaa aatataataa tttcatatta
      541 atcttttcca atcactagat attctttata tgtactctta atatatatag gagtttgata
      601 aatattttta ataattttta tttgttttaa aaataattta cagtttaatt acaaatttaa
      661 gtaattaaaa gacttacatc tttctcattt agtttgaatg ttaagttttt tattttactt
      721 ttataaaaaa ttaatttgcg tcctttatta ttaagagtaa ttttatcgat tgataaaata
      781 cctagatctt ctaacttttt tattctttta taaattgaac ttaacggaag agatatttct
      841 ttatgtattt ggtaaactgt tttagaattt tcgataactg ataaaataat tttatatgta
      901 tcattttcaa ggatattatt taaaaaacaa atagtttcat ttttatctat atcacaacta
      961 ttcttgattt cagttaggct atacatatta ggtcaaacta attaggttaa actaatttat
     1021 aaatgtagcc ttacgaataa tagcaattat ttttcaaaat cagacataga tttacgagat
     1081 gattcgataa gtttcttttt ccgtggctga ataacaagaa tataggccga accgactaga
     1141 tatacagata atagaattag ttgagcaaca acggtttcga gagttggatg tataccagtc
     1201 atagtagcta gattaacatc taaccgtgga attattccga acaaatgtgt ggttgttata
     1261 tatccaactt cttgaaactc acgaatagca tttcccaaaa aggttataga catatatgca
     1321 cctatcccca tggttaaacc aaatagaact cttaacggta gcttcttgcc cagttttcta
     1381 actattaatg ctactcctat tataactgct aggccgataa tcattcccaa aataacaaaa
     1441 agctccatat gtttagcgta agataacatt gcttgataaa acaagacagt ttcaaatcct
     1501 tctctaaata ctgtaaaaaa agacagcaag acaaatacca tcacactgcc agttgtagtt
     1561 gctttccaaa cttttgactt aacgaattct atccattttt ttgtctctat tttgttaaga
     1621 acccaaaaac taacccagaa taatacagct acagcagaaa ttcctgcgat agcttcgata
     1681 agttctctac ttgcaccaga cagctcgata aagaattctg caatcatcca tgtaagagct
     1741 gttgcacctg cagcgagaat tataccataa tatacatgtt ttttgaatcg ctcatttctt
     1801 gaagcttcta aatatgtaat tattgcccct atgattaatg cagattctaa tccctctcta
     1861 aatataatag aaaaggatga tgaaaaggca atacctggtg ctattgtacc aattcccgaa
     1921 actagccttt cagattcatc taatcctttt cgaatttcac caatcttttt tacaatattt
     1981 tcatattctg acttttgttc tatcatgttt ctgagttcag caaatttaat ttccatatct
     2041 aaagtaaaat caggatcgat tggtctcaga ggtaattcta tattctcata actatctaag
     2101 taagcagaac gggctactac taaagcttca tcatatttct cctctttgta taaattcaat
     2161 accttttgaa gatttaatct aatttgatct atatcatttc taacaccttg tttttgagga
     2221 ttagtagatt cacccatctc tcgacgatct ccttgaaagg atccaaaacc tttactaagt
     2281 tcttcaatat tggcaaggtt attcctagaa gaaattaatt ctgttgtatt attattgtta
     2341 ttattattgt tgactattga tgagtcattt tctaaaatta atttagcatt atctagttta
     2401 gtttttattt gagtaataaa tggttcaata acttctaatt tttcattatt tttaataagt
     2461 tttcggagat cttctctcat gtcaatttct atatcaacca taagatttgg atcatttttt
     2521 tcaataggag cttcgaggta ttcataatta tccaaataag cggacatggc atgcttttca
     2581 gcttgtctgt aatcattgtt ttctcttatt tcaacaagca cattacttaa taatgtgtca
     2641 atagttgaaa agtaattttg atattcatat ggaagatttt gtgaattatt gttagtattt
     2701 aataattctt tcaaatagtt ttgaagagag gtaatagatg aagaaacttc attaaaattg
     2761 ttttgattca aattttgttg tattttcgaa tatagtaaaa ttacttggtt catttcgtta
     2821 ccattaaatt catttgatgg aatttgattg aattgatatt gtgatctttc taatattcct
     2881 tttatattat catattggag atttttttgt tcctgatttt gtgatgaatt agtaatactt
     2941 atcaagttag aagcatcttg taataactgg attgagcttt gaattagaaa attcttgtca
     3001 ctgagagtat tatttcctac tatttgacta tttagtaaat aattagtatt tctgatttca
     3061 tctattgttt gtttaatttc ctctataggt ttagttttaa catcctttag aaaaataggc
     3121 aaatcagtta acagtttctc taattgagaa gataattctg gagcagcgaa ttgcaaatca
     3181 gattttatat ttggaaaaat atttgtatgt ggaatatagg catgggaaaa tgctttatcc
     3241 agattgtttg atgctatgtt tttctcacta agatctaatt gagaatttat tctttcaaga
     3301 ttaatatata taataaattt cttttttata tctagtggag aatctaaatc ctcacttaca
     3361 gagtcacttt gtgaatatcc attattgagg aataagagtg taattaagaa aaatactata
     3421 aatgaaaata cgtaatataa tttgattgta tttatttttg aatttatttt atttaccatt
     3481 tgaatttttc ctcggataat gcggtatata ttaggtttac ctaactcttt agctatattt
     3541 aaatatatag aatatttcta aataatgtga tatctgaaat aatgtactta ttatcattca
     3601 ttaagggttc tatgctacaa acatgtttct tttatttgaa atattaaaca ctaatgacag
     3661 atccttgtag tgttaaattg tgatttataa ccgataataa agattttaat tttaataata
     3721 gtttccttta caaccaagat atagaatatt tacgtttgta aaaggacatg aatcaaatcc
     3781 ttgtttcaaa ttccatatag aaatatatta ttctttaatt aattaaaaaa atcagttaac
     3841 aataaaacga ttgtttgatc tcgcagagta tgtcttttac attcatatct atttcacttg
     3901 gatctatttt ggaattcata cctttttgca gctcctttac ctctgtaagg taattcttat
     3961 cattatctga caagtaagtt tgtgacgtat ttaaaagaat taaagctctt ttaaaaaact
     4021 gttgaccatc ctgatattct aggaaattaa tgatctttcc ctgactcatt gattcggtat
     4081 attcctcaga tgcaatagtt attaaatctg cagtaacatt gagtatatga tctcgattag
     4141 ttcttttttc taaagatata gttttatcta gtgcattatt aaggaatttc tctaattcca
     4201 ttttctctat aataatttta tctaaatcat ctgattttac tgtttgaaca tacttgttta
     4261 gtcggtttga gaaatctttt ggtaatgttt cat
//
LOCUS       DATAFW010000096         2009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1809597, whole genome shotgun sequence.
ACCESSION   DATAFW010000096 DATAFW010000000
VERSION     DATAFW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809597"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /gene="guaA"
                     /locus_tag="VHJ57_02315"
     CDS             complement(<1..1387)
                     /gene="guaA"
                     /locus_tag="VHJ57_02315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX2487400.1"
                     /translation="MEIELKLDKIIILDFGSQYSHLICRRIRDANVFCQIYPYNLTSE
                     QIKELSPNGIVFSGGPASVYAKNSPKPDKKIFELGIPILGICYGHQIIVDNFKGKVKR
                     SHTREYGKAILTIDDNSDLFINLDKNIKCWMSHGDVAEKTPRGFKVLGHTESSFAAAI
                     GNQKKKLYGIQFHPEVAHTEHGFEILKNFSINISKAKPEWDMRNFVDSTIENIKKKVG
                     NDKILCAVSGGIDSTTVAVLLSRAVHNNLQCIFVNHGLLRYGEESMVMNLLKQIGLNV
                     TYIDASEKFLNKLKGITDPEEKRRIIGYEFINVFQEEVKDKEQFRWLAQGTLYPDVIE
                     SGISVGPASIIKTHHNVGGLPTVLNLKVLEPLRHLYKDEVKKVAKILEVPEELLNRHP
                     FPGPGLAVRIIGEITPEKIRISKHASKIVEDELTTENLYNSVWQAYAAVGDDKAVGVI
                     GDERLYGHIVTI"
     gene            complement(1371..>2009)
                     /locus_tag="VHJ57_02320"
     CDS             complement(1371..>2009)
                     /locus_tag="VHJ57_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487401.1"
                     /translation="AADLRDWVLKQLSDKQEEIDRLKRLLLLIDNLLKQESFKPASKL
                     QRSINNTESTIIGERQEPVMRNRSTIKNESKKHTLNSLKENVSTFTETRELKRLKDHL
                     LLAKVNISPETIEIIPEKDLLFNIDTPPFKSFFIKRILEGMITQDQDKNRQKQSLKND
                     SLNYTIDEHEGKINKIIINKYREKDRLTDIINTSAWVFTRMLEKENIFGNRT"
BASE COUNT          594 a          363 c          278 g          774 t
ORIGIN      
        1 ttatagtcac aatatggcca tatagccgtt cgtctcctat tacccctacg gctttatcat
       61 cgcctacagc tgcataggct tgccaaacag aattatataa attttcagta gtaagctcat
      121 cctcaacaat tttacttgca tgttttgaaa ttcttatttt ttctggtgta atttctccta
      181 ttattctaac agccaatcct ggtccaggaa acggatgtct atttaaaagc tcctctggaa
      241 cctctaggat cttagcgact tttttaactt catctttata aagatgtctc aaaggctcta
      301 ataccttaag attaagtaca gtaggaaggc ctccaacatt atgatgagtc tttattattg
      361 atgctggtcc tactgaaata ccactttcta taacgtcagg atataaagtt ccttgtgcta
      421 gccatcgaaa ttgttctttg tctttcactt cctcttgaaa aacattaata aattcgtaac
      481 ctattatcct cctcttttct tccggatctg taattccttt taatttgttt aagaattttt
      541 cagaagcatc aatatatgtt acattcagac ctatttgttt taaaaggttc attaccatag
      601 actcttcacc atatcgaagc aaaccatgat taacaaagat acattgcaaa ttgttatgga
      661 cagcacgact taatagaact gctacagtag tagaatctat acctccacta actgcacata
      721 atattttatc atttcctact tttttcttta tattctcaat agtagagtcg acaaaatttc
      781 tcatatccca ttcaggtttt gctttactaa tattgataga aaaattcttc aaaatttcaa
      841 aaccatgctc agtatgagca acttcaggat gaaactgaat gccatacaat ttcttttttt
      901 gattccctat tgctgcagcg aaagaacttt cggtgtgacc taaaactttg aatcctctag
      961 gagttttttc tgctacatca ccatgactca tccaacattt tatatttttg tctaaattaa
     1021 taaaaagatc tgaattatca tctattgtca atattgcttt tccatattct ctggtatggg
     1081 atcttttaac cttccctttg aaattatcaa caattatttg gtgtccataa catattccta
     1141 gaattggtat tccaagttca aagatttttt tatcaggttt gggagaattt ttagcgtata
     1201 cactagctgg gccaccagaa aaaactatac catttggcga tagctcctta atttgttcag
     1261 atgtgagatt atacggataa atttgacaaa aaacattcgc atctctgatt cttctacata
     1321 taagatgact gtattgagat ccaaaatcta gaataattat tttatccaat ttaagttcta
     1381 tttccaaaaa tattctcttt ttctaacatc ctcgtaaaca cccatgcaga tgtattaata
     1441 atatcagtta gtctgtcttt ttctctatat ttgtttatta ttattttatt aattttacct
     1501 tcatgttcat caatagtata gttcaaagag tcatttttta aggactgttt ttggcgattt
     1561 ttatcttgat cttgagtgat cattccttct agaattcttt ttataaaaaa tgatttgaat
     1621 ggaggagtat caatattaaa taataaatcc ttttcaggaa ttatttcaat tgtttctggg
     1681 gaaatattta cttttgctaa taataaatga tctttcagtc tcttaagttc tcttgtttca
     1741 gtaaatgtgg acacattttc ttttaaacta ttgagagtat gttttttgga ctcatttttt
     1801 atagtagatc tattacgcat aacaggttct tgcctttcgc cgataatagt actttcagtg
     1861 ttatttatag aacgttggag ttttgaagca ggtttaaaac tttcctgttt caataagtta
     1921 tcgataagta gaagaagacg tttaagtctg tctatttctt cttgcttatc tgagagctgc
     1981 tttaataccc agtctctcaa gtctgcagc
//
LOCUS       DATAFW010000097         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1458958, whole genome shotgun sequence.
ACCESSION   DATAFW010000097 DATAFW010000000
VERSION     DATAFW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458958"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VHJ57_02325"
     CDS             <1..352
                     /locus_tag="VHJ57_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487402.1"
                     /translation="KFFTHLGFTFNPQFTDENATCMIIGKDSFVMLLVEKFFKTFTNK
                     EICDATTDTEVILALSAESREQVDNMITKVNEAGGREYMKSQDYGWMYHRGFEDINGH
                     IWEIVYIDESSIPK"
     gene            complement(732..1523)
                     /locus_tag="VHJ57_02330"
     CDS             complement(732..1523)
                     /locus_tag="VHJ57_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487403.1"
                     /translation="MEKIKLLFSCPHNGRNEGTATDPPIIERISSHFPPNICPPEDGQ
                     GFSEENDLLTRELTEKIVENIQSLSGKDPYTVYADFRREFIDYNRNERCAFEQSSVKA
                     KEEYLKYHNEISQTIKEMLPEGDTGLAFLFDIHGTDKEKSPETDPEPNFIEVLIGTDQ
                     TLSREALTKKDPDYFWDRKNGLIPLLGTKRIRAFPENESQELENHPLDGGHTIKTYGS
                     GGEPRPGLVAIQIEVIHCIRDNRYCREKFAADMAECILKFVSPFI"
     gene            complement(1905..3005)
                     /locus_tag="VHJ57_02335"
     CDS             complement(1905..3005)
                     /locus_tag="VHJ57_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX2487404.1"
                     /translation="MRAVIFEKQGFDNLKIKDDVQNPEVLDYDVLIKVIVAAVNPIDY
                     YVISGYRTIHQQKKKNLQLKRKPPKTSELPVNPIPHIPGAEFVGVVEKIGSHVKTIKE
                     GDRVAIYNRIFDNTCDMCISGNEMLCRTGGLVSVVNNGGFSEYISIHERNVFKIPDNI
                     DWNTSVSLPVSGLTAFHALKEAQLKINDSLLIFGASGNTGMFAVQLGKRMASRVIAVS
                     TKEWIKEEYGADYLITDYKHIIDKVKEITNGKMADIVINSLGKDTWNDSFSSLGINGR
                     WVSFGTMTGSNVELKINELYSKQIKLIGSNGGNIEEFRELIEFVSYSNTIGNPLKIKV
                     WKRFNLENIDQAFLSLIDKKREGRVLLDINKE"
BASE COUNT         1019 a          515 c          398 g         1103 t
ORIGIN      
        1 caagtttttt actcatcttg gttttacatt taatcctcaa tttactgatg aaaatgctac
       61 atgtatgatt attggtaaag attcgtttgt aatgctctta gttgaaaaat tttttaaaac
      121 ttttaccaat aaagagattt gcgatgctac aacagatact gaggtaatat tggcattatc
      181 tgcagaaagc agagagcaag ttgataatat gataacaaag gtaaatgaag ctggaggcag
      241 agagtatatg aaatcacaag attatggatg gatgtatcat cgtggttttg aagacatcaa
      301 tggacacatc tgggaaatag tttacatcga tgaaagttca ataccaaagt aaaagatact
      361 aagaatactt tcatttaggt aaaaaatcaa tatcaaagca gaaaaggtaa atattaattt
      421 cataaaagaa ttaatcttat cctaaatatt gttatatgca actgctacct ataagtttta
      481 tagtaggacc aaaaattatg tcaaattcgt ttcacatcct actaaacaaa tacaaaaata
      541 ttgcaaatcc attgtatatg agataaattc tgaaataata atataatcaa ggaaaaatat
      601 ttactcaaat gaaaattgac attggtaaaa atggaaataa attagtaaat atacttcata
      661 gctataaaat aagttataaa aacacacaaa aaattattgt aacaaatata ttataacaat
      721 ttgatttacc attagataaa tggagaaaca aacttcaaaa tacattcggc catatctgca
      781 gcaaattttt cacggcaata ccgattatct cggatacaat gaataacctc aatctgtatt
      841 gctactaatc ctggtcttgg ttcacctcca ctaccatacg tttttatggt gtgtcctccg
      901 tctagcggat gattctccag ttcttgactt tcattttctg ggaatgctct tattcttttt
      961 gttcctaata acggtattag gccatttttg cggtcccaga aataatcagg atcctttttg
     1021 gtcaatgctt ccctcgagag tgtttgatca gtcccaataa gtacctcaat aaaattaggt
     1081 tcaggatcag tttcaggcga cttttctttg tcagtaccat gaatatcaaa aagaaaggcc
     1141 aaccctgtgt caccttcagg aagcatttct ttaattgttt gtgaaatttc attgtggtat
     1201 tttaggtatt cttccttagc cttaacgctt gactgttcaa atgcacatct ttcgtttcta
     1261 ttataatcaa taaattccct ccgaaaatct gcgtatactg tatatggatc ttttccgctt
     1321 aaactttgta tattttcaac gattttctca gtgagctctc tagtcaataa atcattttct
     1381 tcactaaatc cttgtccatc ctcaggcgga cagatattag gaggaaagtg actactgatt
     1441 ctctctatta taggtggatc tgttgctgtt ccctcatttc tcccattatg tggacatgaa
     1501 aataataatt ttattttttc catataatca tttgtttatg aaataattaa atattaataa
     1561 acattattag acatttagtt ataagctata aaaagtttga taatttgctt aatcgaaata
     1621 tttttccata atatcataga atatagctcc aattaaaact atcttaagaa caatctagat
     1681 tatagattct gtgaatattc tgagttttgg tttgaaaaaa tctacatatt cttacaaaag
     1741 tttaatgtaa tgcattatta taacatattc taatactaaa tagaaatata atgcagacct
     1801 aaattggttt aataaaagag cattcatagt taatattgat ataaaaaatt tctaattctt
     1861 ttatgttttt atataccatt cctaatcgtt cttatattgt ataattattc tttatttata
     1921 tctaatagta ctcttccctc tcttttcttg tcaatgagtg atagaaaagc ttgatcaata
     1981 ttttcaaggt taaatctttt ccaaacttta atttttaagg gattaccaat tgtattagaa
     2041 taagacacaa attctattaa ctctctaaat tcttctatat ttcctccgtt agatccaatt
     2101 aactttatct gttttgaata taattcgttt atctttaact ctacattact tccagtcatc
     2161 gtaccaaaag aaacccatct accattaatt ccaagagaag aaaaactgtc attccatgta
     2221 tctttgccta aagaatttat tacaatatct gccatctttc cattagttat ctcttttact
     2281 ttgtctataa tatgtttata atcagtaatt aggtaatctg caccatactc ttctttaatc
     2341 cattcctttg tcgaaaccgc aataactcta gacgccattc tcttacctaa ttgtactgca
     2401 aacatacctg tatttccaga tgcaccaaaa atgagtagcg agtcatttat cttcaattgt
     2461 gcctctttca aggcatgaaa tgctgttaat cctgatactg gcaaacttac agatgtattc
     2521 caatcaatat tgtctggaat tttaaagaca tttctttcat gtatggaaat atactcagaa
     2581 aagcctccgt tatttacaac actcacaagt ccaccagttc tacaaagcat ttcattacca
     2641 cttatacaca tatcacaagt attatcgaaa attctattat agatagcaac tctatctcct
     2701 tcctttatag ttttgacatg agatcctatc ttttcaacta ctcccacaaa ctctgctcca
     2761 ggaatatgag gaataggatt tactggtagt tctgatgtct taggaggttt acgttttagt
     2821 tgtaggtttt ttttcttttg ttgatgtatt gttctatacc cagagatcac ataataatca
     2881 ataggattta ctgcagctac tataacttta attaatacat cataatctaa aacttctgga
     2941 ttttgaacat catctttgat tttgagatta tcaaatcctt gcttctcaaa aatgactgct
     3001 ctcacaattc atactattaa ttatagaatt taagt
//
LOCUS       DATAFW010000098         2355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1810930, whole genome shotgun sequence.
ACCESSION   DATAFW010000098 DATAFW010000000
VERSION     DATAFW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810930"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..165
                     /locus_tag="VHJ57_02340"
     CDS             16..165
                     /locus_tag="VHJ57_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX2487405.1"
                     /translation="MTIGERLKVVADDPQSEEDISRWVNRNGHDLISVNKNESDLEFV
                     IKKAK"
     gene            167..1165
                     /locus_tag="VHJ57_02345"
     CDS             167..1165
                     /locus_tag="VHJ57_02345"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX2487406.1"
                     /translation="MTQTITRNQVIEKIGNTPLIELSSFSTDNIKLFAKLEWQNPFGS
                     VKDRAAFWMIKDAESKGRIKKNKSIIIEPTSGNTGIALTGISSSLGYKVEIVIPEKVS
                     AETKLILQKLGAIIHETSDDLCPRVGAGTDQSIALAKAISTPRPDIYYMPNQYENDAN
                     FMAHYETTGPEIWYQTNGRITHFLTGCGTGGTITGTGVYLKEKNPNINVVAIQAQRNH
                     LLQGLRNFEESSMPDLFKRRESVVNEWFTATNKDSFTLVKELANKENMLVGPSSGSVM
                     SAMLETRNKIEKGIIVGIFADDGRKFKSLYTEQNVFTATEYDEALHNAKYLVELLY"
     gene            complement(1343..>2355)
                     /gene="trxB"
                     /locus_tag="VHJ57_02350"
     CDS             complement(1343..>2355)
                     /gene="trxB"
                     /locus_tag="VHJ57_02350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX2487407.1"
                     /translation="YRMQVNDKNNEIAHDNKDSTNNQKLRTFDVIIIGSGPAGYTAAT
                     YTSRAKLKTLIITGSLPGGQLMTTSEVENYPGFPNGIFGPELMMNMRQQAERFGSVIV
                     DDEVIEVDFKKKPFFVKTYSTEYLSNAILVCTGATPRKLGIVGEDEFGGRGVSYCATC
                     DGPFFKGEEIAVVGGGDTALEEATFLTKFGKSVKLIHRRNALKASKILQEKSFENPKI
                     QFMWDSTITKIKGNKKISTISVKNVNTREEQELDVGGLFIAIGHEPNTKIFKNQLEMD
                     DHGYIVLKEYSRTSVEGVFAAGDVHDFRYRQAVTAAGYGCMAALDVEKWLTENKTNNN
                     KL"
BASE COUNT          835 a          370 c          378 g          772 t
ORIGIN      
        1 atagaaatgg aaaaaatgac tataggtgaa agattaaagg ttgtcgctga tgatccacaa
       61 tcagaagaag atatttctag atgggtaaac agaaatggac atgatttaat atctgtaaat
      121 aaaaatgaat cagatttaga atttgtaata aaaaaggcaa aataaaatga ctcaaacaat
      181 aacaagaaat caagtcatag aaaaaatagg aaatactcct ttaatagaat tatcttcttt
      241 ttcaacagat aatataaaac tttttgctaa attagaatgg cagaatccct ttggttctgt
      301 taaggacaga gctgcatttt ggatgatcaa agatgcagaa tcgaaaggaa gaatcaagaa
      361 aaacaaaagc ataattatcg aacctacttc aggaaataca ggaatagcat taacaggaat
      421 ttcatcatcg ttgggctaca aggttgaaat agttattcca gaaaaagtaa gtgctgaaac
      481 gaaattaata cttcaaaaat taggagctat aatacatgaa acatcagacg atttatgtcc
      541 gagagtggga gcaggaactg atcagagtat tgcacttgca aaggcgatat ctactccaag
      601 gccagatatt tactacatgc caaatcaata tgagaatgat gctaatttta tggctcatta
      661 tgaaactaca ggtcctgaaa tatggtatca aactaatggt aggattactc attttctaac
      721 aggttgtgga actggaggaa ctataacagg tactggagtc tatttaaaag aaaagaatcc
      781 taatatcaat gttgttgcaa ttcaagcaca aagaaatcat ttattgcaag gattaagaaa
      841 ttttgaagaa tcttctatgc ctgatctttt taaaagacgt gagagcgtag taaatgaatg
      901 gtttactgca actaataaag attcttttac actggtaaaa gaacttgcta acaaagaaaa
      961 tatgttagtt gggccatcgt ctggttcagt aatgtcagca atgttagaaa ctagaaataa
     1021 aattgaaaaa ggaataatag taggcatttt tgctgatgac ggaagaaaat tcaagagtct
     1081 atatacagaa caaaatgttt ttacagcaac agaatatgat gaggctctgc ataatgcaaa
     1141 atatctcgta gaattattat actagaggta aaaaagctaa gtttttttat taattattaa
     1201 aagtgttgaa taaaatagta atgaagtata aaaaacatta ttctctaggg attcaaattt
     1261 ttaattaatt atttttgtat ttgtatttta ttattcactt acaactaaat tgaataattt
     1321 ttaaaatttt attattacta aattataatt tattattatt ggttttgttt tctgttagcc
     1381 atttttcaac atctagggca gccatacaac catatccagc agcagtaaca gcttgcctat
     1441 aacgaaaatc atgtacatca cctgcagcaa atactccctc aacactggtt cttgaatatt
     1501 cttttagaac aatatagccg tggtcatcca tctcaagttg atttttaaat attttagtat
     1561 taggctcgtg tccaatggct atgaatagac ctccaacatc taattcttgt tcttctctgg
     1621 tattcacatt ttttactgat attgttgaaa tttttttgtt accctttatt ttagtaattg
     1681 tactatccca cataaattga atttttggat tctcaaatga tttttcctgt agtattttgc
     1741 ttgctttaag agcattacgt ctatggatta atttcactga ttttccaaat tttgttagaa
     1801 aggtagcctc ttcaagtgca gtatctccac ctccaacaac cgcaatctct tctcccttaa
     1861 agaaaggacc atcacaggta gcacaatatg aaacgcctct tccaccaaat tcatcttctc
     1921 ctacaatacc taattttcgt ggagtagctc ctgtacatac aaggattgcg ttagataaat
     1981 attccgtaga ataagttttt acaaaaaaag gttttttttt gaaatcaacc tctatcactt
     2041 cgtcatccac aataacagaa ccaaatcttt cagcttgttg tctcatattc atcattaact
     2101 ctggtccaaa aatgccattt ggaaatccag ggtaattttc aacttcgcta gttgtcatta
     2161 actgtccacc aggtaaactt ccagttatta ttaaagtctt tagtttagca cgggatgtat
     2221 atgtagctgc agtgtaccca gcaggaccag aaccaatgat aataacgtca aaagttctta
     2281 atttttgatt attagtagaa tctttattgt catgagcaat ctcattgttc ttatcattga
     2341 cttgcattct gtatg
//
LOCUS       DATAFW010000099         5595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_580986, whole genome shotgun sequence.
ACCESSION   DATAFW010000099 DATAFW010000000
VERSION     DATAFW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580986"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..651
                     /locus_tag="VHJ57_02355"
     CDS             268..651
                     /locus_tag="VHJ57_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487408.1"
                     /translation="MDDFEKNFPKFDWKNTPAYKHPLGRDCPCPKHEYIREQISIAKN
                     LSESKSNNKNRAQITLKFCPDHYNVYFKEVIPSMPLKHRIMTKFALKLGAISIQKIPY
                     MQSDQCFYCKFGSGGFDKRQELPPI"
     gene            complement(648..1349)
                     /locus_tag="VHJ57_02360"
     CDS             complement(648..1349)
                     /locus_tag="VHJ57_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487409.1"
                     /translation="MYFINWERKITTNLFGSNKVVVKKTKSFTNLRHFILIFCFTLIS
                     IFSGNPSSPPGIGQKIIENESFEFRNKLKSMGISTPKLFSISYDEIIEEYIKGGNLYF
                     YFSTKTAHDLAFQAGKITGLLHKKGFVFIDNKCQNFLVNKDKEVIRIDLGLIQKTEAV
                     FAQSMDVGSFLASILDLNPQIYRKVAKGFLSGYIQITSTKIPYLSIILRNILALGFVY
                     NHDNMIKNMVNKRIA"
     gene            complement(1400..1473)
                     /locus_tag="VHJ57_02365"
     tRNA            complement(1400..1473)
                     /locus_tag="VHJ57_02365"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1437..1439),aa:Ala,seq:ggc)
     gene            1554..1979
                     /locus_tag="VHJ57_02370"
     CDS             1554..1979
                     /locus_tag="VHJ57_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2487410.1"
                     /translation="MNIEKSVGAVIKYQSPSSKMKDPQFLLLRNRRGYWGFPQGHKEE
                     GETEIQTLFREVYEETGINYLNILSFIGRIRYSFFKGEGVKSRKEVSFYYATTSIRDI
                     TLSNEHDDFIWTTFSESLTYLNRKQLRSIMIEGHEKGFY"
     gene            2064..3641
                     /locus_tag="VHJ57_02375"
     CDS             2064..3641
                     /locus_tag="VHJ57_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX2487411.1"
                     /translation="MTASGKRIVLTADRSLMTNYRGNFLYGFIACGPYELVPEFVFDK
                     LFCPSVETDKHSGEVKVAQCGLRRIEGALLQDYERNEIFLAHPEMLNKCIGPETRVVG
                     INVMDPLGMAPVTTTMSPEKLSYVAMKFKKMCASIIQLKKRYNFKVVVGGNGAWELAK
                     PDRMKIHGIDTVVIGEADELALDLFHDLEQGDAPELLHTFVRNIENIPTIQGPTINSL
                     IEAMRGCGRGCDFCDVNKRSKKDIPLSRLTEEAKINLNYGFDSIWLQSDEMLLYGCDN
                     KDFFPNADAIIDVWSGLKSVGANFVGTTHMTLSAVASSPDLIKKLSQINDMENNGRWL
                     ATNLGVETVAPRMVKKHLGVKTKPFQPEEWGSVVREGCKILNSNHWFPALTLIIGWPD
                     ETPDETQYTIDLIEDFKHMRMRGLVAPLLYQDFSEKNSMHFGNLNEAQFTLFWKSWQH
                     NLRVINDIIPIIIRNKSYGPPMKLFMASLIKVGTWAIMRYLRGLSKDLFEGKLPEDII
                     EQYSKNRSVTTPLPSKL"
     gene            complement(3664..4002)
                     /locus_tag="VHJ57_02380"
     CDS             complement(3664..4002)
                     /locus_tag="VHJ57_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487412.1"
                     /translation="MKFEYEEFSSIEDLFLYMASIAPYMKQILPITYYKGYLFSIVPI
                     SQLSTEILMMVYVKKEMSTGLFEFDISTGKYKKIQSIDRADKNYFIIITPKSATLADK
                     AIANLKINDR"
     gene            complement(4024..4866)
                     /locus_tag="VHJ57_02385"
     CDS             complement(4024..4866)
                     /locus_tag="VHJ57_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX2487413.1"
                     /translation="MVVENKGIISYIRILKEDLEIIRIIPEGGTVPDFKAGQFVTLSL
                     NNPNEGKLVRRAYSIASPPEKKKYFELLIRWVKKPLPGRLTTQLFNRNEGDDIGWLKP
                     TGIFTVNEKLHDGSPDNRRLVLVGGGTGLAPFISYSLHLKAIGSKREIIVLHGASYVD
                     ELSYRELLTDLEEESIDKGPDVWNFKYRASISRPNEPFNRSWGGQKGRVESFLRPEPG
                     SDTSTLEQIVGEKITPENTSFYVCGWQGTVDGVLDFLKPKGFVDERHKRSDGSYEIKF
                     ESYG"
     gene            4978..5280
                     /locus_tag="VHJ57_02390"
     CDS             4978..5280
                     /locus_tag="VHJ57_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487414.1"
                     /translation="MINWSNIKDFNKSKNTDLVNSINEQKLEQKKVVVLFEEKMKIFA
                     TERSIESSLDILNISIQLANIRGNLIRSFEQYVRNLEEEILKLNRIIEKTPKSPDS"
     gene            complement(5275..>5595)
                     /locus_tag="VHJ57_02395"
     CDS             complement(5275..>5595)
                     /locus_tag="VHJ57_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX2487415.1"
                     /translation="PCGPWITTRDEIIDPQNLKILTMVNGEVRQNSTSSKMVIPIKKI
                     ISSLSQLMTLEPGDIISTGTPAGVAMSMNNPKYLKDGDLVEISVEKLGTIRNKVIFID
                     KVKL"
BASE COUNT         1902 a          883 c          887 g         1923 t
ORIGIN      
        1 atgagttctt gaaatctaaa agagttttaa gattggattc cagtgttaca agattttgtt
       61 catgatcact ccttttagaa ttgatacctt cactgctcaa agcattaatt tcttcggttt
      121 tattcacgcc gcgttaaatc taaatctgta ctaaaattat aaaatatata tttttatcac
      181 ttgttaacat tattatttac attgttcggt agatagaatt catcctataa tttatatgat
      241 attgaattta tattacaact aagaagaatg gatgatttcg aaaaaaattt tccaaagttt
      301 gattggaaaa atactcctgc atataaacat cctttaggaa gagactgccc ttgtcctaag
      361 catgaatata ttagagagca aatcagtatt gcgaagaacc ttagtgagtc aaagtcaaat
      421 aataaaaacc gtgctcaaat aactttaaaa ttttgtcctg atcattacaa tgtatatttt
      481 aaagaggtta tcccttctat gcccttaaag catagaatca tgacaaaatt tgctttaaag
      541 cttggagcta ttagtattca gaaaatacca tacatgcaat cagatcaatg tttctattgc
      601 aaatttgggt ctggaggttt tgataaaaga caagaacttc cacctatcta ggcaatcctc
      661 ttatttacca tattttttat catattgtca tgattataaa caaatcctaa ggctagtata
      721 tttcttaaaa taattgatag atatggtatt ttcgtacttg ttatttgtat atagccagac
      781 aaaaatcctt ttgctacctt tctatatatt tgaggattta agtctagtat acttgctaga
      841 aaactgccta catccatgct ttgagcaaat acagcttcag ttttctgaat taagccgaga
      901 tcgattctta ttacctcttt atctttattt actaaaaaat tttggcattt gttatcaata
      961 aatacaaatc cttttttgtg taataatcca gtaattttgc cagcttgaaa tgcgagatca
     1021 tgtgctgttt ttgtactaaa ataaaaatac agatttcctc ccttaatata ttcttctatt
     1081 atttcgtcat atgatattga aaaaagcttc ggagtactaa tacccattga tttcaattta
     1141 tttctaaatt caaaactctc attctcaatt attttttgtc cgatcccagg aggagaggat
     1201 ggatttccag agaaaataga gattagggta aaacaaaaaa ttaaaatgaa atgtctgaga
     1261 tttgtaaatg acttagtctt ttttactact actttattac ttccaaatag attcgttgta
     1321 atctttcttt cccaattaat aaaatacaat agaagtttta ttattttggt aatatatttt
     1381 aattatacct ttagaaaagt ggaccgggtg ggattcgaac ccacgatctc agcaatgcca
     1441 atgccgtatc ctaccagact agacgaccgg ccctatcact aaattaattt aattcgatat
     1501 taatattttt aagaaacaat attataattc tgaattcaaa aaaatagtgt agtgtgaata
     1561 tagaaaaatc tgtaggagca gtcataaagt atcaatcacc ttcatctaaa atgaaagatc
     1621 cacaatttct actattaaga aatcgtagag gatattgggg ctttccgcaa ggtcataagg
     1681 aagaaggaga aactgagatc caaactctat ttagagaagt ctatgaagaa acagggataa
     1741 attatttaaa tatattatct tttattggca gaattagata ttcatttttc aaaggtgaag
     1801 gtgtcaaatc tagaaaagaa gttagtttct attatgctac tactagtatt agagatataa
     1861 ctctttcaaa tgaacatgat gattttattt ggacaacatt ttcagaatct ttaacatatc
     1921 taaatagaaa gcaattaaga tcaattatga tagaaggaca tgaaaaagga ttttattgaa
     1981 tatttccaac actcactcat agtaagctta tgataataaa tttatatctg attctccgag
     2041 gatgacttga gaggattatt gtaatgactg cttctggtaa aagaatagtt ttaactgcag
     2101 atagaagttt aatgactaac tatcgaggta attttcttta cggttttata gcatgtggcc
     2161 catatgaatt ggtgccagaa tttgtttttg ataaactttt ttgtcctagt gtagaaacag
     2221 ataaacattc aggagaagtg aaggtagctc aatgtggatt gagaagaata gaaggtgctt
     2281 tacttcaaga ctatgagaga aatgaaattt ttcttgcaca tccagaaatg ttaaataaat
     2341 gtattggacc cgagacgaga gtcgtaggaa ttaatgtaat ggatccttta ggtatggctc
     2401 cagttacaac cacaatgtct cctgaaaagt tatcttatgt agctatgaag ttcaagaaaa
     2461 tgtgcgcttc tattatacag ttaaaaaaaa gatataattt caaagtcgta gtaggtggga
     2521 atggtgcttg ggaactagca aaaccagata gaatgaaaat tcatggaatt gatactgtgg
     2581 ttatcggaga agctgatgaa ttagctttgg atttatttca tgacttggaa caaggggatg
     2641 ctccagagct tttacataca tttgttcgaa atattgagaa tattcctacg atacaaggtc
     2701 ctacaattaa ttctcttatt gaagcaatga gaggatgcgg acgaggttgt gatttttgcg
     2761 atgttaataa aaggtcaaaa aaagatattc ctctttctag attaactgaa gaagcaaaaa
     2821 ttaatcttaa ctatggattt gactcaatat ggcttcaatc tgatgaaatg ttattatatg
     2881 gatgtgataa taaggatttc tttcctaatg cagatgccat tatagatgtc tggagtgggt
     2941 taaaaagtgt aggagcaaat tttgttggaa ctactcatat gaccctttct gcagttgcat
     3001 catcacctga tttgataaaa aaactttcac aaataaatga tatggaaaac aatggaagat
     3061 ggttagctac caatctagga gttgaaacag ttgctccaag aatggttaaa aaacacttag
     3121 gggtaaaaac caaaccattt caaccggagg aatggggttc agttgttagg gaagggtgta
     3181 aaattttaaa tagtaatcat tggtttcctg ctttaactct tattattgga tggcctgatg
     3241 aaacacctga cgaaacccaa tatactattg atttaattga agattttaaa catatgagaa
     3301 tgcgaggact agtagctcca ttactttatc aggatttttc agagaaaaat tctatgcatt
     3361 ttggtaatct aaatgaagct caatttactc ttttttggaa gtcttggcag cataatcttc
     3421 gcgttattaa tgatattata ccaattataa tcagaaataa aagttatgga ccccctatga
     3481 agttatttat ggctagtttg attaaagtag gtacctgggc aataatgaga taccttagag
     3541 gtctttctaa ggaccttttt gagggtaaat taccagaaga tattatagaa caatattcca
     3601 aaaatcgatc ggtaaccact ccgttaccat ctaagctata aaatagtatt taattttctt
     3661 tatttaccga tcattaattt tcaaatttgc aatcgcttta tctgctaagg tagctgattt
     3721 tggagtaatg attataaaat aatttttatc tgcacgatct attgattgga tttttttata
     3781 tttcccagtt gaaatatcaa attcaaataa gcctgtagac atttccttct tgacataaac
     3841 catcattaat atctcggtag aaagttgtga gattggaaca atagaaaata aataaccttt
     3901 gtagtatgtt attggtaata tttgtttcat atagggagct atagatgcca tatagagaaa
     3961 caaatcttct atggatgaaa attcctcata ttcaaatttc atacaatatc acaaataatt
     4021 acattatcca taggactcaa atttgatctc ataactccca tcggatcgtt tatgcctttc
     4081 atcaacaaag cctttaggtt tcaagaaatc taacacacca tctactgtac cttgccaacc
     4141 acaaacataa aatgaagtat tttcaggagt tattttctct ccaacaatct gttctaaagt
     4201 tgatgtatcg ctgcctggtt cgggtcttaa aaatgattca acccttcctt tttgtccacc
     4261 ccatgaccta ttaaaaggtt cgtttggtct gctaatactt gctctatatt taaaattcca
     4321 tacatctggt cctttatcaa tactttcttc ttccagatca gttaacaact ctctatagct
     4381 gagttcatcg acataactcg caccatgtaa aacaattatt tctcttttac taccaatagc
     4441 tttgagatgt aaactatatg aaataaacgg tgctaatcca gttcctcctc ccactaaaac
     4501 aagtctccta ttatctggac ttccatcatg cagtttttca ttcactgtaa atatacctgt
     4561 tggcttcaac cacccaatat cgtcgccttc gttacgatta aaaagttgag tagttagcct
     4621 cccgggcaga ggtttcttta cccatctaat cagtaattca aaatattttt tcttctctgg
     4681 aggagaagct attgaataag ctctcctaac caattttcct tcatttggat tattgaggga
     4741 tagggtaaca aattggcctg ccttgaaatc cggaactgtt ccaccctctg gtatgatacg
     4801 tataatctca agatcttctt ttaaaatcct aatataagaa ataattcctt tattctccac
     4861 aaccataggt aatatcgaaa atagtctcac attagtcctt atatatgtta ttataatatt
     4921 atacgcaatt attgacccga aggtttattt tttgttattc attagaaaat attaaaaatg
     4981 atcaattggt caaatattaa agattttaac aaatccaaaa acacagattt agtaaattct
     5041 attaatgaac aaaaattaga acaaaaaaaa gtagtagttc tttttgaaga gaaaatgaaa
     5101 atatttgcca cagaaagatc gattgaatct agtttagata tattgaatat atctatacag
     5161 ttagccaata tacgtggaaa tttaattcgt tcgtttgaac aatacgttag gaacttggaa
     5221 gaagaaatat taaaactcaa tagaattata gaaaaaactc cgaaaagtcc tgattcatag
     5281 ctttacctta tctataaaaa ttactttgtt tcgtatggta ccaagttttt ctacactaat
     5341 ttctaccaaa tccccatctt tcaaatattt tggattattc atggacattg ctacacctgc
     5401 tggtgtgcct gtggaaataa tatcgcctgg ttcaagagtc attaactgac tcaaactaga
     5461 gattattttt ttaataggta tgaccatttt actgctagta gaattctgtc gtacttcgcc
     5521 gttaaccata gtcaatattt ttaagttttg tgggtcaata atttcatctc ttgtcgttat
     5581 ccatggtcca caagg
//
LOCUS       DATAFW010000100         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4809431, whole genome shotgun sequence.
ACCESSION   DATAFW010000100 DATAFW010000000
VERSION     DATAFW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4809431"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..339)
                     /locus_tag="VHJ57_02400"
     CDS             complement(121..339)
                     /locus_tag="VHJ57_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487416.1"
                     /translation="MGLLISTGFLELRLLLLLTPLLMCPVIAVFLGWALLHESTSILN
                     IIAMVVILAGAALMVTSLGKHNYHKIQK"
     gene            complement(663..>1777)
                     /locus_tag="VHJ57_02405"
     CDS             complement(663..>1777)
                     /locus_tag="VHJ57_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX2487417.1"
                     /translation="KKTNKQKEENIEFRRIESLSEIKTACIILDKKDVIIIELKNDSK
                     DNFLEAIGSSIYSNTPTTVFSYISIFDTLWSQTDLYNSIRIANDNLQSTTEELRESLQ
                     ILANVNQELTLTNELLNHHDRMQKEFINVAAHELRTPTQSIIGYCEMIDAFPENTAKY
                     LDPVKRNAEKLYRLTEDILDVSRIESGTLKLEKSKFDLREIIVNLIEDLTAKMKKEES
                     AIRSNQENPDKNTNIQLIYPELSAEPLFVYADKNRIQQVLSNLLSNALKFTEDGVITI
                     NTEKFLDKINNNANNEENEFVSIKIKDTGKGIDPEVLPRLFEKFATKSDKGTGLGLYI
                     SKNIIDAHGGKIWGHDNNKNGKNGAEFGFILPLYNI"
BASE COUNT          552 a          283 c          245 g          697 t
ORIGIN      
        1 ttgcctggaa gtggaagata aataatggat ttgttcatta ctcatcctag attttgatag
       61 tctttgttat cattacttta atttataatt cctatattat tcaaataaga tccttccttc
      121 tcatttttga attttatgat agttgtgttt tcctagagat gtaaccatta atgctgctcc
      181 cgctaaaatt actaccatcg ctattatatt caaaatactt gtagactcat gaagtagagc
      241 ccaacctaga aatactgcga taacaggaca cataagcaaa ggtgttagca agagaaggag
      301 ccgtaattct aagaagccag tagaaattag taaacccaac tactttaatg attgctatta
      361 aataaatttg agctatcaag gactgaccag atattagctc cgagaaatct atatgattat
      421 attctcctaa agaaacaccc acaatgctga gcattaatcc tccactaagc attatcatac
      481 ctgaagaagc aaatacacca gagaaggaac aataagtagc ataaggcctg caaatcccgc
      541 taccagcccc aatataattg atttaggcat aggttttcta tatataaaaa atgaaattaa
      601 tgctatccat agaggaattg tagaatatag attaatcaaa tttatatatg agaatttaaa
      661 ttttatatat tgtataaagg taaaataaaa ccaaattctg caccattctt tccattttta
      721 ttgttatcat gtccccatat ctttcctcca tgagcatcta tgatattttt tgaaatgtac
      781 aaacccaaac ctgttccttt atcagatttt gttgcaaatt tctcaaatag tcggggtaat
      841 acctcagggt ctattccttt tccggtatct tttattttga tagacacaaa ttcgttttct
      901 tcattatttg cattattatt aattttatcc aggaattttt ctgtattaat tgtaattact
      961 ccatcttctg taaattttaa agcattgctt aataaattag aaagtacttg ctgtattcta
     1021 tttttgtctg cataaacaaa taaaggctct gcagataatt ccggatatat tagttgaata
     1081 ttagtatttt tatcaggatt ttcttggttt gatcttattg cactctcttc ctttttcatc
     1141 tttgctgtta gatcttcaat taaatttact attatttctc ttaaatcaaa tttgcttttt
     1201 tcgagtttta aagtaccact ttctatcctt gatacatcta agatatcttc agttaatcta
     1261 tataattttt cagcattcct ttttacaggg tctaaatatt ttgctgtatt ttcaggaaaa
     1321 gcatctatca tttcacaata tcctataata gattgagtag gagttcttag ttcatgggct
     1381 gcgacattta tgaattcttt ttgcattcta tcgtggtgat ttaatagttc attggttaat
     1441 gtaagttctt gatttacgtt tgctaatatt tgtaacgatt ctctcaattc ttctgtggta
     1501 ctctgcaggt tatcatttgc gattctaata ctattatata agtctgtttg gctccaaaga
     1561 gtatcaaaaa ttgagatata ggaaaaaaca gtagttggag tatttgaata tattgatgat
     1621 ccaattgctt ccaaaaaatt atctttagaa tcatttttta attcaataat tattacatct
     1681 tttttgtcta ggattatgca tgcagtcttg atttcggata atgattctat tcttctaaat
     1741 tcaatatttt cttctttttg tttgtttgtt tttttta
//
LOCUS       DATAFW010000101         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_758056, whole genome shotgun sequence.
ACCESSION   DATAFW010000101 DATAFW010000000
VERSION     DATAFW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758056"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VHJ57_02410"
     CDS             complement(<1..1065)
                     /locus_tag="VHJ57_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX2487418.1"
                     /translation="MQNSLNYIVSKSEIGYILNAVLNGDRLNLHDCKELIKSDDLYTI
                     GLIASTLRKILFGNTTTFINNIILNYTNVCVTYCKFCAFYRPPGHEESYTHNIKDIMK
                     RIALANEMFGITQILFQGGHNPNLSIEYYEEIFHEIKKFYPSIAIHGLSASEIDMISK
                     VERSSIKEVISRLKCAGLDSLPGAGAEILDDKVKSVISPLKIKSNQWLNIMKEAHDLG
                     IKSSATMMYGTVESDNEIAQHIMKISELQQKTNGFIAFIPWGFEPNKTQMQTEGIAKY
                     PSGGLRLLKMIAISRIMFYGLISHIQSSWLTNGIGMAQLALHFGADDFGGTLIGEEVV
                     SATGARSTELTSMKIIESIKQ"
     gene            1171..2283
                     /locus_tag="VHJ57_02415"
     CDS             1171..2283
                     /locus_tag="VHJ57_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HEX2487419.1"
                     /translation="MITNSINSIISQPTQAIEKVLSGAELTYNDGLQLFEENLFILGS
                     VADQIRRQSKGNTVTFVSSYYLNYTNVCAASCSLCAFYRKGHEDDAYTLTNEQIVERA
                     RIAIEQMGASELHIVGGFHPKLGLEYYENMIKNIKKEFPFVKVKALTPAEIFFISKLT
                     KNSVKEILLRLKDSGLDALPGGGAEIFSSDIRNIIVRGKCSGEEWLDTAKQAHEIGLK
                     TNSTMLFGHIEKPEHIIDHIIKIRDLNKKTHGFNTFIPLKFSLENTELEKKKLIVRES
                     PSTYDLRIIAIARLLLKDVLDNISVYWVSLGKKIAQVALTYGGNDLVGTAFSEEIFRA
                     AGKTSGISIQELVNLIKEIKRKPAQRDTFFKVIEKY"
     gene            complement(2429..3256)
                     /locus_tag="VHJ57_02420"
     CDS             complement(2429..3256)
                     /locus_tag="VHJ57_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX2487420.1"
                     /translation="MIDITLGHTPDADDAFMFYGLASGNVESNEFHITHVIEDIEKLN
                     KRALNNELDITAISVYAYAFLKDYIILRSGGSFGLNYGPIILAKEMFLKDDLNKKIIA
                     IPGKMTSAYLLLSLLLGNFKEKELPFHIIPQAIIDNQVDAGLVIHEIQLTYKSLNLHK
                     IIDLGSWWNKLTNGLPVPLGINVANNKTLTLEQIRRFDYVFKNSIIYGLNNIEEALEY
                     AMKYGRGQSKDMISKFIKMYVNEITVDMGERGKTSIERIFQLASEKKIISPFQIYFA"
BASE COUNT         1262 a          498 c          478 g         1289 t
ORIGIN      
        1 ttgtttaata gattcaataa ttttcataga agtaagttca gttgatcgtg ctccagttgc
       61 actaactacc tcttctccta tcaatgttcc accaaaatca tcagcaccaa aatgaagagc
      121 caattgtgcc attccaattc catttgtaag ccatgatgat tgaatatgag agattaggcc
      181 ataaaacatg attcttgata ttgcaatcat ttttaatagt cttaatcctc cagaaggata
      241 tttcgcaatt ccttctgttt gcatttgagt cttattaggt tcaaatcccc aaggaataaa
      301 agctataaat ccattcgttt tttgttgtaa ttcagatatt ttcattatat gttgtgctat
      361 ttcattatca gattctaccg ttccatacat cattgttgcg gaagacttga ttccaagatc
      421 atgtgcctcc ttcataatgt ttagccattg gttacttttt atcttaagag gacttattac
      481 tgattttact ttatcatcaa gaatttctgc tcctgctcct ggtaatgaat ctaatcctgc
      541 acattttagt ctagatatta cttccttgat actacttctt tcgactttag atatcatatc
      601 aatttcagaa gcagaaagac catgaattgc aattgatgga taaaattttt tgatttcatg
      661 gaatatttct tcataatatt caatagataa atttgggtta tgtcctcctt gaaaaagaat
      721 ttgagtaatt ccaaacattt catttgccaa ggcaattctc ttcattatgt ctttgatatt
      781 gtgtgtatag gattcttcat ggccaggagg tcgataaaaa gcacaaaatt tgcagtaggt
      841 tacacataca ttagtataat tcaaaataat attatttata aaagtggttg tattaccaaa
      901 aagaattttt ctaagagtac tagcaataag gccaattgta tatagatcat cagattttat
      961 taattcctta caatcatgaa gattaagtct atctccattt aatacagcat ttaaaatata
     1021 tccaatttcc gattttgaaa caatataatt taaactattt tgcaattgaa aattatagtt
     1081 tgattattct atttattctt tacaagaaca gttatcctct gtaatataga agaatatata
     1141 tcaattaata aataatttat tataattaaa ttgattacta atagtattaa tagtattata
     1201 tcacaaccta cacaagctat tgaaaaagta ttatctggag cggaattaac gtataatgat
     1261 ggtttacaat tatttgaaga aaatctattc atattaggtt ctgttgctga tcaaattaga
     1321 agacaatcaa aaggaaatac agtgactttt gtttcatcat attatttaaa ttatactaat
     1381 gtttgtgccg caagttgttc tttatgtgct ttttatagga aaggacatga agatgatgct
     1441 tatacattaa ctaatgaaca gattgttgaa agagctagaa tagctataga acagatgggt
     1501 gcttcagaat tacacatagt tggaggattt catccaaaac ttggtttaga atattacgaa
     1561 aacatgataa agaatataaa aaaagaattt ccgtttgtaa aagttaaagc tctaactcct
     1621 gctgaaatat ttttcatatc taaattaacc aaaaactcag ttaaagagat tttattaaga
     1681 ttaaaagatt ctggtttgga tgctcttccc ggcggaggag cagaaatttt tagctcagat
     1741 ataagaaata ttattgtaag aggaaaatgc tcaggagaag aatggcttga tacagctaaa
     1801 caagctcatg aaattggttt aaaaactaat tcgactatgc tttttggaca tattgagaag
     1861 ccagaacata taatcgatca tattataaaa attagagatc tcaataaaaa aactcatggt
     1921 ttcaatactt ttataccact taagtttagt ctagaaaata ctgaactaga gaagaaaaaa
     1981 ttgattgtta gagaaagtcc ctctacttat gacttaagaa taatagctat tgctagattg
     2041 ttattaaaag atgttttaga taacatatca gtttattggg tttctctagg taaaaaaata
     2101 gcacaagtag cacttacata tggaggtaat gatttagttg gtacagcttt ttcagaagaa
     2161 atatttcgtg ctgcgggaaa aactagtggt atatcaatac aagaacttgt aaatttgatc
     2221 aaagaaataa aaagaaaacc tgctcaacga gatacatttt ttaaagtaat agaaaaatac
     2281 taatagttat acaagtcaat aatatccaaa aattataaaa tctacaataa actattatta
     2341 cttttttatc atatattcgg ctctaataag ttaagaaact taattaaata agggtgttat
     2401 tttaatctga ttttataaag ttctataact aggcaaaata gatttgaaat ggagatataa
     2461 tctttttttc agatgccaat tgaaagattc gttcaataga tgtttttccc ctttcaccca
     2521 tatcaacagt gatttcattc acatacattt taataaattt agatatcata tcttttgatt
     2581 gacctcttcc atatttcatt gcatattcta atgcttcttc tatattattt agaccataaa
     2641 ttatcgaatt tttaaacaca tagtcaaaac gtctaatctg ttctagtgtt aatgttttat
     2701 tatttgcaac atttataccc aacggtacag gcaaaccgtt agtaagttta ttccaccaag
     2761 atcctaaatc tataatttta tgtagattta atgacttgta tgtaagttga atttcatgta
     2821 ttactaaacc cgcatcgact tgattgtcta ttattgcttg aggtataata tgaaaaggta
     2881 attctttttc tttaaaatta cctaacaaaa gagataataa taaatacgct gaagtcatct
     2941 ttccaggtat agctattatt tttttattta aatcatcttt taaaaacatc tcttttgcta
     3001 aaataattgg accgtaattt agtccaaaac ttcctccact tcgaagaatt atgtaatcct
     3061 taagaaaagc ataggcataa acggatatag ctgttatgtc taactcatta tttaatgcac
     3121 gtttattaag cttttcaata tcttcaatta catgagtaat atgaaattca ttagactcta
     3181 catttccact tgctaatcca taaaacataa aagcatcatc tgcatctgga gtatgtccta
     3241 aagttatatc aatcattgat agttatttag atacaaacga atcttatcgt tatattaatt
     3301 aactgtttgg tgggctatta aatcaaaaaa attttaataa ttaaaagtaa atcaagattt
     3361 atttatatga aatatataat atagttattg aataataaaa attttatttt acaacaattg
     3421 aaatggatat caatatattt tgcaattagt attgtaattt cttttataat tccatttccc
     3481 tattcaataa ttgtaataat tccaatagtt ttatttttaa gctttta
//
LOCUS       DATAFW010000102         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4634392, whole genome shotgun sequence.
ACCESSION   DATAFW010000102 DATAFW010000000
VERSION     DATAFW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634392"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VHJ57_02425"
     CDS             <1..1537
                     /locus_tag="VHJ57_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX2487421.1"
                     /translation="FNENAILITGDYVQHLAALAQGIESVFIKYNNKITHFKFEKFFS
                     HKTMSIHLKEGVYPYAKIGSPGKFELKRLDEKIITNEYLKEIIKEIYEYNPLNTNKFY
                     EINREGATVIQYEKYRITITKSPFSDSTEITIVRPIVHLSLDDYKLSEKLLKRLEEKA
                     EGIVIAGPPGSGKSTIASSLAEYYTKKEKIVKTFESPRDLQVPKEVTQYSPLEGSFEN
                     AVDLLLLVRPDYTIFDEIRRLKDFNVYADLRLSGVGMIGVIHANSPIDAVQRFIGKIE
                     LGMIPHIIDTIIFLKDGNINKVYELKLTVKVPSGMVEEDLARPLVEIFDFENGELEYE
                     IYTYGEENIVVPVKEIVEKKKTNTTSNITRLAEAKIKDVVRRFDPSADIEIISDNKVR
                     VKVGKEIIPKIIGRSGSTVSELENLLGLRIDIEAKTPVLDKEKEIDFQINESGSSIII
                     SADKKEVGSNVGIYINNEFLLSSKIGRKSRIKVDKRSEYGKKLLNAISKSSDIKLFKQ
                     MDK"
     gene            1655..2464
                     /gene="pheA"
                     /locus_tag="VHJ57_02430"
     CDS             1655..2464
                     /gene="pheA"
                     /locus_tag="VHJ57_02430"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX2487422.1"
                     /translation="MRIAFQGERGAYSEMAIMLNFPISTAIPLKTFYDVFENVKNKQN
                     DIAVVPIENSIEGSVSETYDLLLQDKIHVSGEIFQRINHCLIVNKDYKKIINVYSHPQ
                     ALGQCRNYIQSKNLEPIPAFDTAGAVKFIKENKMMKSAAIASRRAAEIYEMEILEEGI
                     EDKKNNFTRFLVISKQSINKPSGKDRTSIIFALEHKPGSLYHVLKEFSRKRINLTRIE
                     SRPTKETPWEYYFYVDFDGHYNDEKVKLTLNRIKTQTKFFKLLGSYQKGIF"
     gene            complement(3734..4195)
                     /locus_tag="VHJ57_02435"
     CDS             complement(3734..4195)
                     /locus_tag="VHJ57_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487423.1"
                     /translation="MPMVSRSVYFYGLLIAGIVFIVIGILSVYYSNSSVQVEVDGIIK
                     PNSVDILSPNMKRGDSAFISASGSLFNMSVTYPNKTEVTLTESSSAYSFNLTAFQDGE
                     YFIEVNNLGTSDILVDGYAFTKGNNLSLIGQIMLIITGIVITALGIRTRQS"
     gene            4669..5439
                     /locus_tag="VHJ57_02440"
     CDS             4669..5439
                     /locus_tag="VHJ57_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2487424.1"
                     /translation="MQYFDAHIHLTDDHFSNYIGYILPSLRSLKIRACSVTVDLTTSK
                     KSLILFNNHNDIITQFIGIHPEFALKENIEEFKKFFISNLKYIDGIGEIGLDPTYVYH
                     NKTQYSIQKKIFQLLLELAEKYNKPISIHSRKSIDEILQTLPSFKIKNSLLHWFNGNK
                     KHLKQAMDMNIFVSYGPVLLYSPDKKVLLSRTYTDKLLVETDGPVRYSNCFNYIPSSS
                     ISHLLSVIKCIGDSLNLNFEEVSNLLKTNSENFLEKII"
BASE COUNT         2205 a          754 c          828 g         1895 t
ORIGIN      
        1 atttaatgaa aatgcaattc taattactgg tgattatgta caacatttag cagcactagc
       61 ccaagggata gaatcggttt ttataaaata taataataaa attactcatt tcaaattcga
      121 aaaatttttt tcacacaaaa caatgagtat ccatctaaaa gaaggagttt atccatatgc
      181 aaaaattgga agcccaggaa aatttgaatt gaaaagatta gatgaaaaga ttataacaaa
      241 cgaatacctt aaggaaataa ttaaagaaat ttatgaatat aatccactga atactaataa
      301 attttatgaa ataaatcgag aaggagctac cgttattcaa tatgaaaaat atagaataac
      361 aattactaaa tctccatttt ctgattctac agaaattact attgtaaggc ctatagtaca
      421 cttatcatta gacgattaca aattaagtga aaaacttctt aagcgattag aagagaaagc
      481 tgaaggaatt gttatagctg gtcctcctgg atccggtaaa agtacaatag caagcagtct
      541 agcagaatat tatacaaaaa aagaaaaaat tgtaaaaaca tttgaatcac caagggattt
      601 acaagttccc aaagaagtga cacaatatag tccattggaa ggaagttttg aaaatgcagt
      661 agatttgctg ctactggtca gaccagatta tactattttt gatgaaataa gacgattaaa
      721 agacttcaat gtatatgcag atttgagact atcaggagtt ggaatgattg gagtaattca
      781 tgctaatagt ccaatagacg ctgttcaaag gtttattgga aagatagaac ttggaatgat
      841 tcctcatatt atagatacaa ttatttttct taaagatggt aatataaata aagtttatga
      901 attaaaattg acagttaaag ttccttctgg aatggttgaa gaagatctag ctagaccatt
      961 agtggaaatt tttgattttg aaaatggaga attggaatat gaaatctata cttatggtga
     1021 agaaaatata gtagtacctg taaaggaaat agtagagaag aaaaagacaa atactactag
     1081 taatatcaca agacttgcag aagctaagat aaaagatgta gtaaggagat ttgatcctag
     1141 tgctgatata gaaattatat ccgacaacaa agtaagagta aaagtaggaa aagaaataat
     1201 tccaaaaatc ataggaagaa gtggctcaac tgtctctgaa ctagaaaatc tattaggttt
     1261 aagaattgat atagaagcta aaacaccagt tcttgacaaa gagaaagaga ttgatttcca
     1321 aattaatgaa tctggttcat caatcataat tagtgcagat aaaaaagagg tcggatccaa
     1381 cgttggaatt tatattaata acgaattcct tttaagttca aaaattggta gaaaatcaag
     1441 aatcaaagtt gacaaaagat cagaatatgg aaaaaagcta ctaaatgcaa tatcaaaatc
     1501 atctgatatt aaacttttca aacaaatgga taaatgacaa aaaaatctaa tacaatgaaa
     1561 attatgatga gtacgaactc ttattacaag tctatagttt agaataaaca taaaagattg
     1621 ttctacgatt accaattatt tgactagtaa aagaatgcgg attgcctttc aaggagaaag
     1681 aggggcttat agtgaaatgg caataatgct aaactttcct atttctaccg ctattccttt
     1741 aaaaactttt tatgatgttt ttgaaaatgt taaaaacaaa caaaatgaca ttgcagtggt
     1801 acctattgag aattcgatcg aaggcagtgt aagtgaaaca tatgatttat tattacagga
     1861 caaaatacac gtttcaggag aaatctttca aagaattaat cattgtttga ttgtaaataa
     1921 agattataaa aaaataatca atgtatactc tcatcctcaa gcactaggtc aatgtagaaa
     1981 ttatattcaa agtaaaaact tagagccaat cccagcattt gacactgctg gagctgtaaa
     2041 attcatcaag gaaaacaaaa tgatgaaatc tgcagctatt gcaagtagga gagcagcgga
     2101 aatatatgaa atggagatct tagaagaagg aatagaagat aaaaagaata actttacaag
     2161 gttcttagtt atatcaaaac aatcgataaa taaaccctca ggaaaagatc gaacctcaat
     2221 tatttttgcc ttagaacata aaccaggttc tttatatcac gtattaaagg aattttctcg
     2281 aaaaagaata aatctgacta gaattgaatc tcgtcctacg aaagaaacac catgggaata
     2341 ctatttttat gtagattttg atggacatta taacgatgaa aaagtaaagt tgacattaaa
     2401 tagaattaag actcaaacaa aattttttaa attattagga tcttatcaaa aaggaatttt
     2461 ttaaattatt ttctttttaa tcatttcata ttatttaaaa tataaatttc caaaataatt
     2521 atgtatccgt ttgtatagac aagttatcga cctttagtga gtaaacatat cattgtgttg
     2581 ttctaatatc atagagggaa tgatttccct atcttacgct tatattattt atgataggta
     2641 tgtcatattt aggtttcaat aagattctaa tattacaaat attttaaatt actagttttt
     2701 ataattcact attaaaataa attcccaaat tagttgttca aatgcaaaaa gttagaagta
     2761 tgttcatgaa agattgatta tttaaaaagt agaatcttct cttgatcgat gttgaagaat
     2821 ctggaatagg atatttacct tacaatacgc cattataata taaagaatat tcaataatca
     2881 ttgacattcc cgaatgcaaa tttaaattat gcctattacc actagttctt ctagtaaatc
     2941 cccgtccaca tattatgcat atacacgttt gtttgctagt catatgacaa taattgagtt
     3001 ttagcttata aaaggacaaa aaaagttttc gctatccgtg tcataaagtg ctataaagtc
     3061 taatttttga gtttctgata ccttactaaa atagaggtaa tatgctccca cgagttcgtg
     3121 ttacattaat ttataataaa tgtaacactg tactctatga ctaatcatct ttaggtttta
     3181 ggttagtgtg tgagttgata gttactattt acctaattga tctgtagagg attcatttac
     3241 aatattaaat gaggaagtat cttcatctat atcatctgta tttagtttga tgtttagccg
     3301 ctctgattct gttgtgctgt aataacagaa ctcccttgtg cagaggttgc attagacgat
     3361 tctgcttcct gagcttctgc atttcaactt attacaaaga gaagttatat ttgcatattt
     3421 gatggaataa aatagtaagg tttgaatcat tctctatcta ggaatgtaaa agggttttta
     3481 tatttatagt agatgaaaca atgagcccca taggttctaa atatgtcata ttaaatttta
     3541 ttaaatcagt aaataaatca taattaagaa tattttctaa tgagaaaaga aataagacta
     3601 tagcagaaaa tatactttta tttcaattcc tatctatgaa atatattaca catcgggtac
     3661 taacataatc ttccaatgtt aaaccctaca gaaattaata aatctagaat aatatcaaaa
     3721 aaggattttt aatttagctt tgtcttgttc ttatacctag agcagttatg actattcctg
     3781 taattattaa cattatttga ccaattaaag ataaattgtt tcctttggta aatgcgtaac
     3841 catcaactaa aatgtctgag gttcctaaat tgttaacttc tataaaatat tctccatctt
     3901 gaaaagctgt taaattgaaa gaataagctg atgaagattc tgttaatgta acctcagttt
     3961 tattaggata tgtaacagac atattaaaaa gagatcctga cgccgatata aatgcactat
     4021 ctcccctttt catgttaggc gaaagaatgt caacagaatt gggtttgata ataccatcaa
     4081 cctctacttg aacagaagaa tttgaataat aaaccgataa aatcccaata acaataaaca
     4141 ctatccctgc tatcaataaa ccatagaaat acacagatct tgataccata ggcataacaa
     4201 tttagcagta agatattaat acatttcata tctaagtttg ttatttaaag acattttggt
     4261 aaagataatt tatcggtatt caaatacaga tacaaagaac cgaaaattta agtaattcta
     4321 catatttatc ttcaatcttt atttagtcat gcttatatac atatttcaaa aatcggtgag
     4381 tattttttca tcttatcatt tggtttataa aaataaagaa tgaatttcaa tcgaatcctt
     4441 caacatagtt gatatctcat ctttttcgtt atactattcg tcaaatactt tgagatacaa
     4501 attatattag gaatacggga aattcaaatt tatgaatttt acattcttag gtatagctac
     4561 tttagtaata atatgagtta aaaaattaat cgtcacctta tatagttcgt tatttaaggt
     4621 aagaattaca aacttattta agttattatc gtagatttta tttttcatat gcaatatttc
     4681 gatgctcata tacatttaac tgatgatcac ttttcaaatt atataggtta catccttcct
     4741 tcgttaagat ctttaaaaat cagggcatgt tctgtgactg tagatcttac aacttctaaa
     4801 aaaagtttaa ttttgtttaa taatcacaat gatataataa ctcaattcat tggaattcat
     4861 cctgaatttg cacttaaaga gaatatagag gaatttaaaa aattttttat ttctaatcta
     4921 aaatacatag atggaattgg agaaattgga ttggatccca catatgttta tcataataag
     4981 acacaatatt ctatccaaaa gaaaatcttc caactactat tggaacttgc tgaaaaatat
     5041 aacaaaccta tttcaattca ttccagaaag tcaatagatg aaatattaca gacattaccc
     5101 tcctttaaaa tcaaaaattc tttattacat tggttcaatg gaaataaaaa gcatctaaaa
     5161 caagctatgg atatgaatat ttttgtatct tatggaccag ttctattata ttctccagat
     5221 aagaaagttc tcttaagtag gacatatact gataaactat tagtagagac tgatggtcca
     5281 gtaaggtatt caaactgttt taactatata ccttcatcat caatatcaca tcttttaagt
     5341 gtaattaaat gcataggtga tagcttaaat ctgaattttg aagaggtatc aaatttattg
     5401 aaaacaaatt cagaaaactt tttagaaaaa attatttgaa aaatgaagaa atattatgca
     5461 attaatagta atagtgacca tttttctcaa attatatttt gatataaata aataccaaat
     5521 ttgattagca ttattttgaa aactatattc attctataat tcttggctgt atcacctgtt
     5581 tttaattaac aaaaattatt gtggtgttgg aggtaaaatc tctcttacag ataaaaattt
     5641 gaatgataca tcagattgat cccatgtaaa tacactcttt gg
//
LOCUS       DATAFW010000103         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1287053, whole genome shotgun sequence.
ACCESSION   DATAFW010000103 DATAFW010000000
VERSION     DATAFW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287053"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..891)
                     /locus_tag="VHJ57_02445"
     CDS             complement(82..891)
                     /locus_tag="VHJ57_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HEX2487425.1"
                     /translation="MEDQKNLPEEGDIVIANVKQVTNHGAYVYLEEYDNLTGFLHISE
                     IATGWIRNIERYVRPNQKIVLKVIRVNKARSEVDLSLKQVSGEEKKSKIIEVKKNEKA
                     NAFMAIIKSKANLSNEQIKQIEEKILTKFDYVYDLFEATTKKGKEVLLPLELAENVIN
                     IIEEESKKIPIPHVEIRGIVEVSSQKSNGVEIIKSILSTAEITKGNSKVMISYIAAPK
                     YRIVVAADNFKIAEKSINGAIEKIKSAIEKENGHFNFIRQESKKTQQQLVH"
     gene            complement(954..1490)
                     /gene="cobO"
                     /locus_tag="VHJ57_02450"
     CDS             complement(954..1490)
                     /gene="cobO"
                     /locus_tag="VHJ57_02450"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX2487426.1"
                     /translation="MDSDNGIVVIYTGKGKGKTTAALGIVLRAVGHGFKVLMIQFIKG
                     EWYYGELSSAKRLTPEFEMIAAGKGFIGILDDDHDFVEHRKSAEDALILAKEKIISKE
                     YDIIILDEINYALNLQLLSINQVLELIKIRPKQTSIVLTGNYAPDEIIQVADLVTEMN
                     EIKHPYQQGIKAKRGIDY"
BASE COUNT          486 a          264 c          204 g          669 t
ORIGIN      
        1 gttatagccg aacaattagg acattgattc ttaagagtat aaattttaca ttttaaacat
       61 tttctcaata aatgtttcat tttaatgaac taattgttgt tgagtttttt tagattcttg
      121 acgaataaaa ttaaagtgac cgttctcttt ttcaattgca gattttattt tttctatagc
      181 accattaata gatttctcag caattttaaa attatctgca gctacaacta ttctatattt
      241 aggtgctgcg atataagaaa tcataacctt tgaattacct tttgtaattt ctgcggtact
      301 taaaatagat ttaataattt ccacaccatt ggatttttga gatgaaactt ctactattcc
      361 tcttatttca acatgaggga tgggaatttt tttactttcc tcttcaatta tatttattac
      421 attttcggct aattctagtg gcaatagtac ttctttccct ttttttgtgg tagcttcaaa
      481 aagatcataa acataatcaa atttagtaag tattttttct tcaatttgtt ttatttgttc
      541 attcgaaaga ttggctttac tttttattat tgccatgaaa gcattagcct tttcgttttt
      601 ctttacctca ataatctttg attttttttc ttcacctgat acttgtttca gtgataaatc
      661 aacttccgat cgtgctttat ttactctaat aacttttaaa acaatttttt gattaggtct
      721 aacatatctt tcaatatttc ttatccaacc tgttgctatt tcagaaatat gtaaaaatcc
      781 tgtcaaatta tcgtattctt ctaaatatac atatgctcca tgatttgtaa cttgtttaac
      841 gttagcaata acaatatccc cctcttcagg gagatttttc tgatcttcca tcttttattt
      901 aactccgata caagctatat ttattttgta aagtatcgtc ttgatcttat caattagtaa
      961 tcaattcctc tttttgcttt gataccttgt tgataaggat gtttaatttc attcatttca
     1021 gtaactaaat cagcgacttg gataatttca tcgggggcat aattaccggt taatacaatg
     1081 gatgtctgtt tggggcgtat cttaattaat tcaagtactt gatttattga aagaagttgg
     1141 agattaagtg cataattaat ttcatcaagg ataataatat catactcttt agatattatt
     1201 ttttccttgg caagaattaa cgcatcctct gcagattttc gatgctctac aaaatcgtga
     1261 tcatcgtcta atattccaat gaaacctttt cctgccgcaa tcatttcaaa ttcaggagtt
     1321 aatctttttg cgcttgaaag ttctccataa taccattcac cctttataaa ttggatcatc
     1381 aaaaccttaa agccatgacc tactgctctc aatacaattc ctaatgcggc agtggttttt
     1441 ccttttcctt taccagtgta aatcactaca attccattat cggagtccat tgttatagag
     1501 gcataaaaag atctaaatta tatttttctg tcttgatata tagaattatt ttttagtaat
     1561 atgtaaatct acaagaataa tgtatgaaaa gtttatattt ttaggattat aatttaatta
     1621 atc
//
LOCUS       DATAFW010000104         8287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6222323, whole genome shotgun sequence.
ACCESSION   DATAFW010000104 DATAFW010000000
VERSION     DATAFW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6222323"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VHJ57_02455"
     CDS             <1..406
                     /locus_tag="VHJ57_02455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX2487427.1"
                     /translation="KENIQGIDFRETDSKQQVDIKISFRNDNGKVAGQTMTNFDSDGF
                     IFNVKILLAEKAFGKQLNKNVIEYIAKHEFGHALGLGHANGNGFLMSSLVYNSFNEIS
                     DCEREAVNEANKWKLIDNDSAPKISEIKKYSC"
     gene            complement(628..903)
                     /locus_tag="VHJ57_02460"
     CDS             complement(628..903)
                     /locus_tag="VHJ57_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2487428.1"
                     /translation="MKYRSRTDIVALILEATKEGAAKTKIMYKAFLSYSQLKDYLSIL
                     LERELIEYSDKDQIFRITDKGRRYLYLYSKVMELITPSDQDLENQIK"
     gene            complement(997..1239)
                     /locus_tag="VHJ57_02465"
     CDS             complement(997..1239)
                     /locus_tag="VHJ57_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEX2487429.1"
                     /translation="MIGETQLRARRTRIKILRAIAERNGSAGFSDIQNATALSTGSIY
                     YHIERMRDYIRKNAKNYSITKKGIQLLVEIDLKNKE"
     gene            1436..1572
                     /locus_tag="VHJ57_02470"
     tRNA            join(1436..1473,1507..1527,1558..1572)
                     /locus_tag="VHJ57_02470"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1470..1472,aa:Trp,seq:cca)
     gene            1705..2808
                     /gene="radA"
                     /locus_tag="VHJ57_02475"
     CDS             1705..2808
                     /gene="radA"
                     /locus_tag="VHJ57_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HEX2487430.1"
                     /translation="MENESKSNANVAIIDLDIQDIEGIGPTTAKKLKDAGILSVMDLA
                     VASAEELSESINATKETAASFIISAQKLLRETNVLDKEFVTADDALQKRRSMLRCSTG
                     SYALDDLLLGGIETQALTEFYGEFGSGKSQVCHTLCVTAKQPIEAGGLNSSVIYIDTE
                     GTFRPERVEQIARSRGFEPTEILKNIAVCKVYNSSHLELIVKDLGKYIDDFKAKLVVI
                     DSIISLHRAEFSGRGTLADRQQRLNGMLHKLIRLAEIYNIAIVITNQVQSSPDTFFGD
                     PTKATGGNVLGHASTYRIYLRKSGENRIAKMMDSPYHPYSDTRFTVTEKGADDIEEQT
                     PKKGSKRSTTTTSASSKKKEEDDEEEEEEAQQD"
     gene            complement(2865..3143)
                     /locus_tag="VHJ57_02480"
     CDS             complement(2865..3143)
                     /locus_tag="VHJ57_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487431.1"
                     /translation="MNYELVIKVGAIIASIGTIFLVIFATVLLVFPITKLCENGPKCI
                     YISNAPYPLNLLIAPIVLVIILVVIACGIAIIRLGKWFFSKKKVYDKQ"
     gene            complement(3225..3827)
                     /locus_tag="VHJ57_02485"
     CDS             complement(3225..3827)
                     /locus_tag="VHJ57_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487432.1"
                     /translation="MQSNLNKLFFIYVAKNEEWEKRQEEDWDYVSSMVKFFHWWTKRN
                     FALNFSINADILPVITGRLFDRMSLAYLLRDHQDRGKSVYHFYLSYFKPFWTDCNVSG
                     YATNNFGMVQWKRPEHIESKEQISKFFADYNCARVSHVLLHEILRLKGKNKKEYFEQV
                     HDLWDAHEHKNLPFIYYNEKYSKVTYNDFYRFVTIDISKL"
     gene            4174..5046
                     /locus_tag="VHJ57_02490"
     CDS             4174..5046
                     /locus_tag="VHJ57_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX2487433.1"
                     /translation="MDDLDLKINDWTVNTVNSLIKPNKLDKIAECALELSKIIYYNNM
                     NVEKYLDEINLMQKELNIKRGRSNINFSRPTQIIELINDYMFKEKEFKANIYDYQNPL
                     NNFLNVVLEKRIGIPITLSIIYITLAQSINFKLYPVNFPRHFLVKYELDDVNNNEIII
                     DPFNQGRIMDDYALKNLMDSFFPNQNIPLTKKLIEKTNLSQVMIRILNNVKDSFFEIQ
                     EFDKLNIANEMIFAIDPQNIYAIRDKGIILHEKDPEKSLEFLNRYLELEPEANDADIV
                     LKIIKSIREEKNNR"
     gene            complement(5138..5302)
                     /locus_tag="VHJ57_02495"
     CDS             complement(5138..5302)
                     /locus_tag="VHJ57_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487434.1"
                     /translation="MTKDKYELSIDEQELQTILDSLSNTIFNEEIKDDARKNAKDLFI
                     KLQKDFPILK"
     gene            complement(5443..5964)
                     /locus_tag="VHJ57_02500"
     CDS             complement(5443..5964)
                     /locus_tag="VHJ57_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX2487435.1"
                     /translation="MQEKNMLNWNKVRPTWDEYFMLQAEIAKLRSNCLTRQIGAVIVR
                     DNRQIATGYNGTPPGILNCIDGGCKRCQERINGKINSGVNLERCLCTHAEANAIMQCA
                     LFGNGSSTKGTTLYSTFAPCLECSKMCITVGIERIVVLHNYPEDGNILLQEANIKLDK
                     LKREDILPWCKFI"
     gene            6081..6641
                     /locus_tag="VHJ57_02505"
     CDS             6081..6641
                     /locus_tag="VHJ57_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487436.1"
                     /translation="MRSSLQKNDEKRARTIFLVVFYFCAITMFLITFYSLYVSIQEYL
                     STGKVVIGEVLVNTEFPFPGLAKLVTYLMIVTVVGWYCVTKLGADKVNTISESIKSIV
                     QLIVLAIMVISLYEFIYNFVIWNSFITVDAINGIIRLDDINVPYPNPNTPWNPVFATK
                     MSLSALLISAHGFYLISRHKRQDQIV"
     gene            complement(6669..8006)
                     /locus_tag="VHJ57_02510"
     CDS             complement(6669..8006)
                     /locus_tag="VHJ57_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX2487437.1"
                     /translation="MISDLKTLINIPSVSAKNQNLVECAEAVFALMNRVGIKSEIIYL
                     DPKDKNIAASSFPPPIVYGEIKSQKNPNGKTILFYNHYDVQPEDPLDQWEVPPFSGEV
                     SGNYIYGRGSSDDKGELITRIKAVEYFLKETGDVPCNIKFIVEGEEEIGSIHIEKYLT
                     KYKEKFACDGIIWEFGYVNEKNIPIISLGMKGLLYVELIAYGPNKDTHSSLAVIVENP
                     AWNLIKLLNSMRDENGRILISDWYNDIRNFNQQEEKILNLELFDANEFKKEYGISRFL
                     GDISGIDVKKALCGSPTCNIAGFVSGYVEQGAKTIIPSFAKVKLDFRLVPDMDPQKQF
                     AKLQQHIKDLGFKNININFIHGEAAARTSPNDPFVKIITESAKRVFGDVILNISSAGT
                     GPMHSFQKILKASSVSIGSTYVYSRIHSPNEFARIDLLINITKCIGEIMENYN"
BASE COUNT         3040 a         1148 c         1157 g         2942 t
ORIGIN      
        1 gaaagaaaac atacaaggaa ttgattttag agagactgat agtaaacaac aagtagatat
       61 caagatttct tttaggaatg ataatggaaa agttgcagga cagaccatga ctaattttga
      121 ttcagatgga tttattttta atgtaaagat attgctagct gaaaaggctt ttggaaaaca
      181 attgaacaaa aatgtgattg aatatatagc aaaacatgag tttggtcatg ctctaggtct
      241 aggacatgca aatggtaatg gatttcttat gtcatctttg gtttataatt catttaacga
      301 gatttcagac tgtgaaagag aagctgttaa cgaagcaaac aaatggaaat taatagacaa
      361 tgactcggct ccgaaaataa gcgaaattaa aaaatattct tgctaattca atttttgcta
      421 attcattgtt tatctcaaat tttaaattat taatcttaat ttttttattt ataaattatg
      481 gtaatatata ttatttatag taatttttta ttattattat tattattatt atatgaccta
      541 atttttacaa attatacaat gtaacaaata taagaataat agacataatt gttatttttt
      601 cttatactat aaagtttatt ttattattta tttaatttga ttttccaaat cttgatcact
      661 tggagtaatt agttccataa ctttagaata aagatacaaa tatcttcttc ctttgtcagt
      721 tatcctaaaa atttgatcct tatcagaata ttctattaat tcacgttcta ataatattga
      781 aagataatct tttaattgac tataagaaag aaaagcctta tacattattt ttgttttagc
      841 tgctccctct ttagtagctt ccagtattaa tgctacgata tcagtcctgc ttcgatattt
      901 cattaacaga ttttataaat tgaacgcttc ataaaaattg atcgtaaagt attaattaaa
      961 attattaata tgaaaaatgt aattgaaaac taattattat tccttatttt ttaaatcaat
     1021 ttctactaat aattggatac ctttttttgt gatagaataa ttctttgcat tttttcttat
     1081 atagtctctc attctttcaa tatgatagta tatagaacct gtagataaag ctgtagcgtt
     1141 ttgtatatca gaaaaaccag cagatccatt tctttcagcg atagcacgta atattttaat
     1201 tcgggtcctt ctggcgcgga gttgggtttc tcctatcaat attgatattg cttaaataaa
     1261 ttatttattt tttactaaat tgaataaaaa tttataataa aaaagtaaag atatgaaaaa
     1321 tgttatacta aatatttgat atgtaacatt tattattact agattatttg tttatatttt
     1381 ctcttcgtaa agcttataga gatatagatc aatatccata actgcgatga catatggggt
     1441 cgtagcgaag cctggcatcg caacgggctc cagatcaaac aaaagggcta gtaattgctt
     1501 gaagataccc gtaggtcaag ggttcaattg ataatggtta aatcactatc agaacaactc
     1561 ccttcggccc catctatata taaaattaaa ttcaattatg aagaatagag gtggaaatta
     1621 acagatatac aaaaaagtat acctccctca ccctctataa agttactgca tatattaata
     1681 tgagaaaatc acttgcatag taatatggaa aatgaatcaa aatcaaatgc taatgttgca
     1741 atcattgatt tagatattca agatatagaa ggtataggtc ctactactgc aaaaaaattg
     1801 aaagatgctg gtatcctttc tgtaatggat ttagctgtag ccagtgcaga ggaactatct
     1861 gaaagtatca atgctacaaa agaaacagca gcttccttta taatttcagc tcaaaaacta
     1921 ttaagagaaa caaatgtctt agataaggaa tttgttactg cagatgatgc gttacaaaaa
     1981 agaagatcaa tgcttagatg ttccacgggt tcttacgcat tagatgatct tttacttgga
     2041 ggaatagaaa cacaggcttt aacagagttc tatggtgaat tcggatcagg taaatctcaa
     2101 gtttgtcata ctttatgtgt aacagcaaaa caaccaattg aggcaggtgg attaaatagc
     2161 tctgttatct atatagatac agaaggtaca tttaggccag aacgagttga acaaatagca
     2221 agatcaagag gatttgagcc tacagaaatt cttaaaaata tagcagtttg caaagtttat
     2281 aacagctctc atcttgaatt aatagttaaa gatttaggaa aatatattga tgattttaaa
     2341 gcaaaattag tagtaattga tagcataata tctcttcata gagctgaatt ttctggaaga
     2401 ggaactttag cggatagaca acaacgatta aacggtatgt tacataaatt aataagatta
     2461 gcagagatat acaacatagc aatagtcata acaaatcaag tacagtcttc acctgatacg
     2521 ttctttggcg atcctacaaa agctacagga ggtaatgttt taggtcatgc ttccacatat
     2581 agaatttatc ttagaaaatc cggagaaaat agaatagcaa aaatgatgga ttcaccatat
     2641 catccttatt cagatacaag gtttacagtt actgaaaaag gagcagacga tatagaagag
     2701 caaacaccga agaagggaag taaaagatca acaacaacaa catcagcatc atcaaagaag
     2761 aaagaagaag atgatgaaga agaagaagaa gaagctcaac aggattaaat aaaaaataat
     2821 aataataatg atggatggac tgtagggtta aattcttata ttttttattg tttatcatat
     2881 accttttttt tactaaaaaa ccattttcct aatcttatta ttgctatacc acaagcgata
     2941 acaacaagaa taattactag aactattgga gcgatcaata aatttaatgg atagggagca
     3001 tttgaaatgt atatacattt aggtccattt tcacataatt ttgtaatagg aaagacaagt
     3061 aatactgttg caaagataac taaaaaaatt gtgccaattg atgcaataat tgcacctact
     3121 ttaattacca attcataatt catattgtaa tcgcatgaag tttattatta ataagttact
     3181 attattattt ttatttttaa tatcatcagt ttcttctgat gtaattagag ctttgatata
     3241 tctattgtta caaatcgata aaaatcatta tatgttacct tagaatattt ttcattataa
     3301 tagataaatg gaagattttt gtgttcatgt gcatcccata aatcgtgaac ttgttcaaaa
     3361 tattcctttt tgttttttcc tttaagtctt aagatttcat gtaataaaac atgtgaaact
     3421 cttgcacaat tataatctgc aaaaaatttt gaaatttgct cttttgattc tatatgttca
     3481 ggtcttttcc attgaaccat accaaagtta tttgtagcat atccagatac attacaatca
     3541 gtccaaaagg gtttgaaata tgatagataa aaatgatata ctgatttacc tctgtcttga
     3601 tgatctctta ataaataagc taatgacatt cgatcaaata atcttccagt gatcacagga
     3661 agaatatcag cattaatact aaaatttaaa gcaaaatttc tttttgtcca ccaatgaaag
     3721 aatttaacca tagaagagac ataatcccaa tcttcttctt gtcttttttc ccattcttca
     3781 ttttttgcga catatatgaa aaaaagttta tttaaattgg attgcatagc cttttgaaaa
     3841 atttctccta attgttatta gaacaactat aacaacaaca acaaccacaa ttgtaataat
     3901 tgattattac aattacaatg atacatatta ttattttgaa aaagagatgt aatatgaggg
     3961 ttaaccatgt atttcatcaa tctataatta tagccaaaag attctctacc actaataata
     4021 ttgcaattgc taggtatctt gaattcatcc atatacatat tcctttgtaa aaatacaata
     4081 aaaaacctat ctaataataa cgttaaccaa taaataataa tgataaaatg gaagttaatc
     4141 aagaatatat tagactacat aatatattaa ttgttggacg atcttgattt aaaaatcaac
     4201 gactggactg taaatacagt taattctcta ataaaaccta acaagcttga taaaattgcg
     4261 gaatgtgctt tagaactttc aaaaataatt tactataata atatgaatgt agaaaaatat
     4321 ttagatgaaa taaatttgat gcaaaaggaa cttaatatta aaagaggaag atctaatatc
     4381 aattttagta gaccgactca aataatagag ttaataaatg attatatgtt taaagaaaaa
     4441 gaattcaaag ctaatatata tgattaccaa aatccattaa ataatttttt aaatgttgtg
     4501 cttgaaaaga gaataggaat tcctattaca ttatcaataa tatatatcac tcttgcacaa
     4561 tcaatcaact ttaaattgta tccggttaat tttccacgac attttctagt taaatatgaa
     4621 ttagatgatg ttaataataa tgagataata attgatccat ttaatcaagg cagaataatg
     4681 gatgattatg ctttaaaaaa cttaatggat agtttttttc caaatcaaaa tattccatta
     4741 acaaaaaaat taattgaaaa aactaattta tcccaagtaa tgatacgaat tttaaataat
     4801 gtaaaagata gcttttttga aattcaagag tttgataaat taaatatagc aaatgaaatg
     4861 atttttgcaa tagatcctca aaatatatat gcaatcaggg acaaaggaat aatattacat
     4921 gagaaagatc cagaaaaatc cttagaattt ttgaatcgat atttagagct cgaaccagaa
     4981 gcaaacgacg ctgatattgt tttaaagatt attaaaagca taagagaaga aaaaaataat
     5041 agataagagt gtatattagg aagtttgtat gtagtagaca cattattatt attattatta
     5101 ttatttagtt tataaataga agaaaaaaag aatagactta ttttaatatt ggaaaatctt
     5161 tttgcaattt tataaataaa tcttttgcat tctttcttgc atcgtcttta atttcttcgt
     5221 taaagatagt attgctaaga gaatccaata tagtttgtag ttcttgttca tctatagaaa
     5281 gctcatattt atctttagtc atatttttca taattcagta taaagtaata taaaattcta
     5341 ttcattatga aaaattatta tgagatggaa taaaaaaata aatatttaca tcatttagca
     5401 gcaggataat atcaatttaa ttattattat aaacaaaatt tattagatga atttacacca
     5461 aggaagaata tcttctctct ttaatttatc aagctttata tttgcttctt gtagtaatat
     5521 gttaccatct tcaggataat tatgtagaac aacaattctt tcgatgccta cagttatgca
     5581 cattttacta cattctaagc aaggtgcaaa tgtactatac aaggttgtac cttttgtgct
     5641 tgaaccgttt ccaaataatg cacattgcat gattgcgtta gcttcagcgt gagtgcataa
     5701 acatctttct aaattaaccc ctgaatttat ttttccatta attctttctt gacatctttt
     5761 gcatccacca tctatacaat taagtattcc aggaggagtt ccattataac ctgttgctat
     5821 ctgccgatta tctctaacta taactgctcc tatttgcctg gtaaggcaat tagatcttaa
     5881 ttttgcaatt tctgcttgaa gcataaaata ttcatcccaa gttggccgta ctttgttcca
     5941 atttaacata tttttttctt gcatacacta attttggatc aactataata atataaacat
     6001 ggatatagta ttataaattc taaaaatttt tatgtgaatt ttataattaa gatcaataag
     6061 ttaggagagt aaataatagt atgcgtagtt ctttacaaaa aaatgatgag aaaagggcaa
     6121 gaacaatctt tcttgttgta ttttactttt gtgctataac aatgttccta attacttttt
     6181 actctttata cgtaagcata caagaatatt taagtacagg aaaagtcgtt ataggtgaag
     6241 ttcttgtcaa tacagaattt ccttttccag gattagcgaa attggtaact tatttaatga
     6301 tagttactgt tgttggttgg tattgcgtta caaaattagg agcagataaa gtaaatacaa
     6361 tttctgaatc gataaaatct attgttcaat taattgttct tgcaataatg gtaatatcgt
     6421 tatatgaatt tatttataat tttgttatat ggaattcatt cattacagta gatgcaataa
     6481 atggaataat aagattagat gatataaatg tgccctatcc taatcctaat acaccatgga
     6541 atccagtatt tgctacaaaa atgtctttat ccgctctatt gatttctgct catggatttt
     6601 atcttatctc aagacataag cgccaagatc aaatagttta atgtggaaaa aaattctatt
     6661 aattacaatt aattatagtt ttccataatt tctcctatac attttgtaat attaattaaa
     6721 agatcaattc ttgcaaattc gtttggagaa tggattctag agtatacata tgtactgcct
     6781 attgaaacag aagatgcttt gagaatcttt tgaaatgagt gcattggtcc agtcccagct
     6841 gatgagatat ttaaaattac atctccaaaa actctttttg ctgactctgt tattatttta
     6901 acaaatggat catttggaga agttctcgcg gctgcttctc catgaatgaa attgatattt
     6961 atatttttga aacctaagtc tttaatatgt tgttgaagtt tagcaaattg tttttgtgga
     7021 tccatatcag gaacaagacg aaagtcaagt ttaacttttg caaatgatgg gattatagtt
     7081 ttagcaccct gttctacata tcctgaaaca aaaccagcaa tattacatgt aggagaacca
     7141 cataatgctt ttttaacatc aattcccgaa atatcgccta gaaatctaga tataccgtat
     7201 tcctttttaa attcatttgc atcaaacaat tctaaattaa gaattttttc ttcttgttga
     7261 ttaaaatttc ttatatcatt ataccagtct gaaattaata ttcgtccatt ttcgtctctc
     7321 atactattaa gtaatttaat taagttccat gctggatttt ccacaattac tgctaaactg
     7381 gaatgagtat ctttatttgg accataagca atcaattcaa catacaataa acctttcatc
     7441 cctaaactta taatgggaat atttttttca tttacatatc caaattccca tataatccca
     7501 tcacaggcaa acttttcttt gtatttggta agatattttt ctatatgtat acttccgatt
     7561 tcttcttctc cttctacgat aaatttaata ttgcatggaa cgtcgcctgt ttcttttagg
     7621 aaatactcta ctgcttttat tctagtaatt aattcgcctt tatcatctga tgaacctctt
     7681 ccgtaaatat aatttccaga aacctcacca ctaaaaggag gaacttccca ttgatctaaa
     7741 ggatcttcag gttgaacatc atagtgatta taaaataaga tagttttgcc gttcggattt
     7801 ttttgtgatt ttatttctcc ataaactata ggaggaggaa aagaagaggc tgcaatattt
     7861 ttatctttag gatcaagata gataatttct gatttaattc ctactctatt cataagagca
     7921 aaaactgcct ctgcacattc aacaagattt tgattttttg cagaaacact aggaatgtta
     7981 atcaatgtct taagatcaga gatcattgta ctaatctctg aatcaacaat tttttctatc
     8041 gagcttacca atatttaaaa tttgtaaaag aataatttaa cttttcataa aaaagtacat
     8101 ttgataacga tatgtttgaa ttatagaata tatttcaata atcgtaacag gaatacatat
     8161 aaccatgccg ttgtgaaacg ataataatga cgtcttcatc attcttatct tttaactcaa
     8221 agaatatttt ctttaatcca gagtcaattg ctgtaattgg ggcttctgaa aagccaggag
     8281 tgggaaa
//
LOCUS       DATAFW010000105         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_407249, whole genome shotgun sequence.
ACCESSION   DATAFW010000105 DATAFW010000000
VERSION     DATAFW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407249"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VHJ57_02515"
     CDS             <1..831
                     /locus_tag="VHJ57_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487438.1"
                     /translation="KLKSEFENLSALLKEVTKIISTKENEINTCIECKQDLILLDEKM
                     KERNAKKVRLIELIEETQTIDKNIMVRKKQYEDFQKSQEFQNTSKILVEIDEKKNEIG
                     AFEKNMINMISNLSRPITKFSYLASKDTQARLATVLDQPLEIFKDNSQYLQLFGELRK
                     NVDEKSIQIKDPEKTIHQIDEIINSLPALSAKLKNLKSQLNQLESSVNANNITHLEDI
                     RNNISISEKNRLENIARTEETESIIAELDTASIALKKKVEERLLELTNTKYSIIQIEN
                     "
BASE COUNT          643 a          253 c          236 g          424 t
ORIGIN      
        1 aagttaaaaa gtgaatttga aaatttatca gccttactaa aagaagtaac taaaataata
       61 tctaccaaag aaaatgaaat aaatacatgc atagaatgta agcaagattt gattttatta
      121 gatgaaaaga tgaaagaaag aaatgctaaa aaagtgagac taatagagtt aatagaagaa
      181 acacaaacca ttgacaagaa tattatggta agaaaaaagc aatatgaaga ttttcagaaa
      241 tctcaagaat ttcaaaacac ttcaaaaatt ttagtagaaa ttgacgaaaa gaaaaatgaa
      301 attggagctt ttgaaaagaa tatgataaac atgatttcta acctatcacg tccaataacc
      361 aaattttctt atcttgcctc taaggacact caagcaaggc ttgcaacagt attagatcag
      421 cctctagaaa tttttaagga taattctcaa tacctgcaac tatttggcga acttaggaaa
      481 aatgtggatg aaaagagcat tcaaattaaa gatccggaaa aaacaatcca tcagatagat
      541 gaaatcataa attctctacc cgcattgtct gcaaagttaa aaaatctaaa atcacaactg
      601 aatcaattag aatcatcagt aaatgcaaat aatattactc atttagaaga tataagaaac
      661 aacattagta taagtgaaaa aaatcgtttg gaaaatattg caagaactga ggaaacagag
      721 agtattatcg ctgagcttga taccgcatca atagcactga aaaaaaaggt ggaagaaaga
      781 ttattggaat taacaaatac aaagtattct attattcaga ttgaaaatta aggccgaatt
      841 gcccataata tgatgaaaat atttcgacct ctgcctgtca ctgtcactaa tgataattta
      901 agttcctgaa tgattcataa attactatca tggaaagaga tatcaaaaaa ttgtcatatc
      961 aattcagcgt cagacgcaat agatacagac accaatttta gtttagcaat tgacctttat
     1021 cagaatattg cttccctcta aataatattt gctagagata aacaccagct gatcttatga
     1081 gtagcaattg atatcaatat tagtaaagat gaatcagaac gatacagaaa ctttctataa
     1141 gtgactaggc gctcaacttg acttacctcc taagtcatgg gcaaaagaga tgttttcgca
     1201 tagagataac taaatgacat tgactacgga aacaatatta aaattgttcc aactttcaat
     1261 tcagtcgttt ataataaaat tacattcgac gagaacaaca aatgcaaaat tctaaacgaa
     1321 aatgttatct ccagaacacc gaaattacgc ctaaacccat aatgcatagg cctccgtagg
     1381 tgaccggtgg cacattcaca aataatcctc ccagtgctac tccaattcca cagcatatag
     1441 tgattaactc accgatcctt cgttgatttt tcatcgtcta aaaaaacctt gcagccagta
     1501 ttataaaagc ctatgtaaac ccgtaatatt cttaagtcat ataggaaata gagaga
//
LOCUS       DATAFW010000106         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1995259, whole genome shotgun sequence.
ACCESSION   DATAFW010000106 DATAFW010000000
VERSION     DATAFW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995259"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            686..895
                     /locus_tag="VHJ57_02520"
     CDS             686..895
                     /locus_tag="VHJ57_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487439.1"
                     /translation="MGTDMKNRQDRSNNNNNDNKNYKKKKLNEKDFLLILTQMQRNHS
                     LIYGSDILLLHGTKCTMNIQNIQQE"
     gene            859..1098
                     /locus_tag="VHJ57_02525"
     CDS             859..1098
                     /locus_tag="VHJ57_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487440.1"
                     /translation="MYNEYSKYTTRMTELYQEYAKSSEKMTELYKEMAINADKMTELY
                     KEFAKSTEKMSKYWLSYISWMKPLSEYKEEKEKQE"
     gene            1161..2075
                     /locus_tag="VHJ57_02530"
     CDS             1161..2075
                     /locus_tag="VHJ57_02530"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX2487441.1"
                     /translation="MNNNTGDTKKNYKVAVVTGSSSGIGLETSLLLARNGFYTYAAMR
                     NPDKSKKEGMVNVANREKLPLKVIPLDVVDDKSVKEAIDRITTEQGRIDVLVNNAGYL
                     LLGPLEELSIEEFKEQFETNFFGAIRVTQAVLPIMRRHKEGVIINISSIAGRIGFPLS
                     SAYVSSKFALEGLSESMAYEVEQFGIKVILVEPGVIKTNFANNIKIGKRVNNSSSHTN
                     TSTNSSSQYSELIRNRIAGFKPRFEGGSAPIEVAKIILKCITSKNSLLRYLVGDDAFK
                     LMDIRKNASDKDFRKLVMDSVIHYEKKQ"
     gene            2155..2670
                     /locus_tag="VHJ57_02535"
     CDS             2155..2670
                     /locus_tag="VHJ57_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487442.1"
                     /translation="MFGKDKKEAQAGKEKQAQNFYTLLQLLMNELFEYASKDGHVKMP
                     YNVSFGLIYDMIYALLFTGVDQKSGKKVYFYNLRIIANTFEQRRSQMMADKTPNSLFN
                     WIYEVCSLYINPFVYEYVVNVQHRGKGAADNTTAHIVRRFMNKLLNDPNLKYVLNTIR
                     DEKKSNNSHIH"
     gene            2723..3235
                     /locus_tag="VHJ57_02540"
     CDS             2723..3235
                     /locus_tag="VHJ57_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487443.1"
                     /translation="MKSLLILRHAKSSLKDPNIADHERPLDELAKKDALQMGKLIRDK
                     DIIPDFILSSTAVRAKTTTELVAEGCEYKGDIVFEQSLYKAKPKDYLKIIEGLSDRYV
                     RILLVGHNPAVEETMEVFTGLLDITMSACALAHLKVQIEKWSDIKQEQNNIRRAELIA
                     VIRPEDYLRR"
BASE COUNT         1323 a          526 c          619 g         1052 t
ORIGIN      
        1 cttcttcagc agaaagatct tctgcataac gtgctacaga tgtatcaact acgtttgtac
       61 tagcgtcttt ttctgataaa tcaccaacaa cttccagaat acttcttaat tttggcttta
      121 ttaccattga atcatattta gcaattgtgg cttatattga tttttcaaac ttgtagggta
      181 tatcaaagca gcttaaacac tgaccctatt tttattgtca ttacatatgc atctttatag
      241 gcttaataaa atatttgttt taaattttca gtaagactgc ttgtttcctg tttatcatat
      301 gattcattcc ttacttttgg gcttacccct agcgattcca attcatttac accaattatc
      361 tcttgtggtc ttggtttgtt ctttacttct accttacaaa agaaggttta ccaaaaagcc
      421 cacaaattaa aaatccctgc aacaaatatt tcttatttat tattttgaat agcaaaacac
      481 atttaccagc aaggttcaaa tccacagaac aattatctgg catataaagg agaaggtctt
      541 gatactgtat tctgctaatc ttataatggg ctacatatac tagtgtattg tgtccccacc
      601 gatgaacaaa caagataagc aactgctttc ctaactacaa atcagaaatg gaaggagttc
      661 ttaaatcaga cttctttata catccatggg gaccgatatg aagaatagac aagacaggag
      721 caataataat aataatgata ataagaatta caagaagaaa aaactgaatg aaaaggactt
      781 tttattgatt ttaacgcaaa tgcagcgaaa tcattcactg atttatggca gcgatattct
      841 cctactgcat ggaacgaaat gtacaatgaa tattcaaaat atacaacaag aatgaccgaa
      901 ttatatcaag aatatgcaaa aagctctgag aagatgacag agctgtacaa agaaatggcc
      961 ataaatgcag ataagatgac tgaattgtat aaggagtttg ccaagagtac agaaaaaatg
     1021 agtaaatatt ggttgagcta tatctcttgg atgaaaccat tgtcagaata taaggaggag
     1081 aaggaaaaac aagaataaat tactgcagtc ataaataatg ttatgtaata tcatctatct
     1141 taatactagg aaatgataaa atgaataata atacaggaga tacgaaaaag aactataagg
     1201 ttgcagtagt aactggtagt tcatctggta taggccttga aacctctttg ctactggcaa
     1261 gaaatggctt ctatacgtat gctgcaatgc gtaatcctga caaatcaaaa aaagaaggaa
     1321 tggttaatgt tgcaaataga gaaaaacttc cgttaaaggt tataccatta gatgtagttg
     1381 atgataaatc cgttaaagag gctattgaca ggataacaac tgaacaagga agaatagatg
     1441 tactagttaa taatgcagga tatttattat taggcccatt agaagagctt tctatcgaag
     1501 aatttaaaga acaatttgaa accaattttt ttggtgctat tagagttaca caagcagtat
     1561 tacctattat gagaagacac aaagaaggtg taataataaa tattagttct atagctggta
     1621 ggataggatt tccattaagt tctgcatatg taagctcaaa atttgcacta gaaggattat
     1681 cagaatctat ggcttacgaa gtagaacaat ttggaataaa ggttatactt gtagaaccag
     1741 gtgtcattaa aaccaatttt gcaaataata tcaagatagg aaaaagggtt aataatagta
     1801 gtagccatac taatactagt actaattcta gttcacaata ttcagaacta atacgaaata
     1861 gaattgcagg ctttaaacca aggtttgaag gtggctcagc accaatagaa gtggctaaaa
     1921 tcattttgaa atgtattacc tcaaaaaatt cacttttgag gtatttggta ggagatgatg
     1981 ctttcaagtt aatggacata agaaagaatg cttctgataa ggactttagg aaattagtaa
     2041 tggatagtgt tatacactat gaaaagaaac aataaattgc agtaatagta tctttcattt
     2101 aattccttat tcttaatagc tttgatatgc ttttaatatc attctaagaa gattatgttt
     2161 ggcaaagaca aaaaggaagc acaggcagga aaggagaaac aagctcaaaa cttctatacg
     2221 ttgttacagt tattaatgaa tgaactattt gaatacgctt ctaaagatgg gcatgttaag
     2281 atgccatata atgtatcttt tggtttaatt tatgatatga tatatgcact attatttact
     2341 ggagtagatc aaaaatctgg caaaaaggta tacttttaca atctgaggat aattgcgaat
     2401 acttttgaac aaagaagaag tcagatgatg gcagacaaaa ctcctaactc tctatttaat
     2461 tggatctatg aagtatgttc tctttatatt aatccgtttg tttatgaata cgtagtaaat
     2521 gtccagcaca gaggaaaagg agctgcagat aatactacag cacatattgt taggagattt
     2581 atgaataaac ttctaaatga cccaaatcta aaatatgtat tgaatactat tagagatgaa
     2641 aagaaatcta ataatagcca tatacattga gaatcagata tacaataaaa aacctgctat
     2701 aggtaattga tataacaaaa aattgaaatc cctactcatt cttcgtcatg caaaatctag
     2761 cttgaaagac ccaaacatag cagatcatga aagaccatta gatgaacttg caaagaagga
     2821 tgcattacaa atgggaaagc taatcagaga taaagatata atcccagatt tcattttaag
     2881 ctcaactgca gtacgggcta aaactacaac agaacttgtg gcagaaggtt gtgaatataa
     2941 aggagatatt gtttttgaac agtcccttta taaggctaaa ccaaaagact acttgaaaat
     3001 catagaagga ctttctgata gatatgtgcg tatattattg gtaggacata acccagcggt
     3061 cgaagaaaca atggaagtat ttacaggttt attagatata acaatgtccg catgtgctct
     3121 agctcatttg aaagtacaga tagagaaatg gtctgatatt aaacaggaac aaaacaatat
     3181 cagaagagca gagttaatag cggttataag accagaggat tatcttagaa gataataata
     3241 ataaatcaat aaactactca acttacgccc aaattgccac cgtctgcaca atatccaaat
     3301 tatcaattca tgaaatacaa atatggggat gataaaggtg ataagtccaa tctatcttgt
     3361 aattatagtt agtttcgata ggattaacat gctaacctta aggtttacat catgtttacc
     3421 aggtgacact gtagtggtgt gctcaaatgg cttagaagca ataataatgg ttgggaggga
     3481 aaattgactc aaatttgtcc cgcatcccac cgaacccgct
//
LOCUS       DATAFW010000107         5197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6225053, whole genome shotgun sequence.
ACCESSION   DATAFW010000107 DATAFW010000000
VERSION     DATAFW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6225053"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VHJ57_02545"
     CDS             <1..281
                     /locus_tag="VHJ57_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX2487444.1"
                     /translation="DHDINVHDINEVIWAITTKSDPKRDIIIIDNTPTDTLDPASPIL
                     NLGSKMGIDATTKTAEEGFVREIQKPVEVDYDTKNLVDKKWIHYGFSN"
     gene            404..811
                     /locus_tag="VHJ57_02550"
     CDS             404..811
                     /locus_tag="VHJ57_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487445.1"
                     /translation="MEFDVKIGATKERVVTTNSNQTTSFLWKGENVLSTPSMISEMEE
                     TCRLLLKDFFLKEKEWDSVGTIVDIKHIAITPVGSKIRLKAIIESVNNRRVLFNVEAF
                     DDIEKIGEGKHERYIINVAKFKSKFEEKKRKLE"
     gene            891..2591
                     /gene="thsB"
                     /locus_tag="VHJ57_02555"
     CDS             891..2591
                     /gene="thsB"
                     /locus_tag="VHJ57_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit beta"
                     /protein_id="HEX2487446.1"
                     /translation="MSMGQIQTSAGGMPVVILREGTRENKGKEAQKNNLTAAKLVAEV
                     VKSSLGPRGMDKMLVDSLGDVTITNDGATILKEIDVQHPAAKMIVEVAKSVDNEVGDG
                     TTSSVIFTGALLEKAEELIDKNVHPSVIVDGYTAASIEALKTLDKIAIKVKIDDRDLL
                     AKIAHTSMYSKLVSEDSPVLSKIVVDATQLIAELNEKTKTLKVDLDNIKVEKKAGGSM
                     QDTSLIKGIVLDKEVVHSGMPKRIENAKIALINSPLEIEKTEMSAEIRISDPQQMQMF
                     LEEENNMLRAMVEKVKSSGSNVLLCQKGIDDLAQHYLAKEGILAVRRVKESDMLKLTK
                     ATGARLVNNLDDLSSKDLGSADLVEERKVETDKWVFIEGCKNPKAVTILVRGGSQRVV
                     DEADRSLHDALMVMKDVLEKPFIVAGGGAPEAYIATYIRNWSSSLEGREQLAVIKFAE
                     ALETIPISLATNAGMDPIDTMAELRAKQNKGLKWTGVNVRSTTVADMFKQNILEPVVI
                     KEQIIKSATEAACMLLRIDDVIASSKSKGGPPGGGMPGGMGGMGGMGGMGGMPGMDME
                     "
     gene            complement(2738..3853)
                     /locus_tag="VHJ57_02560"
     CDS             complement(2738..3853)
                     /locus_tag="VHJ57_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487447.1"
                     /translation="MYYYNIHDIIKVKSEINLQELEYFSSKEFSDPDITLLIRSFSKD
                     SDMSKFRATRKLMTDNFESGGSQYTIKYTEHFGSSGAQFQIKFNGSKIEVSINELISR
                     SKHVTYVNLVEPILRFLFLSKEYILLHSACMSSPTGQGFFLSAPPDTGKTTTVIKCVK
                     HGFYFLSDDMTIINLPNTAVCFPKSMTISAHTYRTAVEAQSSSHNSDNDTNNKKGMRI
                     RSLIHSKTGRSLMHKLADHNVPIFTINTIGQSRIKPPKYHISDIIKDAKIKDKTTINS
                     LFFLEKGSAGEKEEKMIRISVDDALKRAIENSDDAFLFPPYSDLIKYLRIEGKTAYDL
                     LEAEKKMMEKLLSSIDCYILKSSGRKWSDMILDKFST"
     gene            4137..4931
                     /locus_tag="VHJ57_02565"
     CDS             4137..4931
                     /locus_tag="VHJ57_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX2487448.1"
                     /translation="MQTNHHEIFETKNMYTISIIIPTYNEAENIISLLDKIKENLTHN
                     VFTEIIIVDDNSPDGTSNIVQNYIDYDVESKRGNANFKEKLYSLKIINRKIKNGLIPA
                     ILDGVKSSQGDYVLVMDADFSHPPEMIPKIIEKSVGNSNSIVIASRYTKNGSIVGWPF
                     KRRLISKAAAVIAKFGLNVNNVTDPMSGFFAVPRHIIENLKIDTKGYKILLEIIVKSK
                     DIPIFEIPYTFMDRKSGKSKMGFSVIFDYIGSVWQLYRYGRNHQKK"
BASE COUNT         1884 a          725 c          906 g         1682 t
ORIGIN      
        1 ttgatcatga tattaatgta catgatataa atgaagtaat ctgggcaatt actacaaaat
       61 ctgatcctaa aagagatata attataattg ataacactcc tactgatacc cttgatcctg
      121 catctccgat tttaaatctt ggttcaaaaa tgggaataga cgcaactact aaaactgccg
      181 aggaaggatt tgtgcgagaa attcaaaaac cagtagaagt tgactatgat accaagaatt
      241 tagttgataa aaaatggatc cattatgggt tctctaattg aacaattcta ctatcattcg
      301 tttttcaatt tgattcttta caatttgtta tattggttcc aaattatcat tattatatca
      361 attagtttaa taaattcaat catgttttat gttttgattt taattggaat ttgatgtaaa
      421 aattggtgct acaaaagaga gagttgtaac aactaattca aaccagacaa ccagtttttt
      481 atggaaagga gaaaatgtgc tttctactcc ttcaatgatt tctgaaatgg aagaaacctg
      541 tagactgctt ttaaaggatt tttttttaaa agagaaggaa tgggattcag ttggaaccat
      601 tgttgatatt aaacatattg ctataactcc tgtaggttca aaaattaggt tgaaagcaat
      661 aatagaatct gttaataata gaagagtact gtttaatgta gaggcatttg atgatattga
      721 aaaaatcgga gaaggaaaac atgaacgtta tattattaat gtagccaagt ttaaatccaa
      781 atttgaggaa aaaaaaagga aattagagta aataatgata ttaacttcat taatcgtgta
      841 aaactataaa aatagggttt tgaaatgtgt ttattattag gtgaatgaat atgagtatgg
      901 gccaaattca aacaagtgct ggcggaatgc cagtagtaat tttaagagaa ggaacacgtg
      961 aaaataaagg aaaggaggca caaaaaaata atcttactgc cgctaaattg gttgctgaag
     1021 ttgtgaaatc tagtttaggt ccgcgaggga tggataagat gttagttgat tctctaggag
     1081 atgttactat aacaaatgat ggagctacta ttctaaaaga aatagatgtc caacatccag
     1141 cagcaaaaat gatcgtagaa gtagccaaat cagtagataa tgaggtaggc gatggaacca
     1201 catcttcagt gatttttact ggtgcattat tagaaaaagc ggaagaattg attgacaaaa
     1261 atgttcatcc ctcagttatt gttgatggtt atactgctgc ttccattgaa gctcttaaaa
     1321 cattggataa aattgctatt aaagtaaaaa ttgatgatag ggatctttta gcaaaaattg
     1381 cgcatactag catgtattca aagttagttt cagaagatag tcctgtctta agtaaaatag
     1441 tagtagacgc tacacaactg atagctgaat tgaatgaaaa aacaaaaact ctcaaagtcg
     1501 atcttgataa catcaaagta gaaaaaaaag caggaggttc tatgcaggat acttccctta
     1561 taaaaggtat agttctcgat aaagaagttg ttcatagtgg tatgcctaaa agaattgaaa
     1621 acgcgaaaat agcattgatt aattctccat tagaaataga aaaaactgaa atgagtgcag
     1681 aaattcgtat tagtgatcct cagcaaatgc aaatgttcct tgaagaggaa aataatatgc
     1741 taagagctat ggtagaaaaa gttaaaagtt caggctcgaa tgttttacta tgtcaaaaag
     1801 gaatagatga tctcgcacaa cactatcttg ctaaagaggg tatacttgca gttagacgtg
     1861 ttaaagaaag tgatatgcta aaactaacta aagccacagg tgcaagactt gtaaataatc
     1921 ttgatgatct ttcttcaaaa gaccttggtt cagcagacct agttgaagaa cgaaaagttg
     1981 aaactgataa atgggttttc atagaaggat gtaaaaatcc aaaggcagtc actattctag
     2041 ttagaggtgg atctcaaaga gtggtagatg aagccgaccg ttctcttcat gatgcactga
     2101 tggttatgaa agacgttttg gaaaaaccat ttatagtggc aggtggaggg gcacccgaag
     2161 cttacatagc cacatatata cgaaattggt ctagcagttt agaaggtagg gaacaattag
     2221 cggtaattaa atttgcagaa gcattagaaa ctattccaat atctcttgct actaatgctg
     2281 gtatggatcc aatcgacaca atggcagagt taagagcaaa acaaaacaaa ggactgaaat
     2341 ggactggtgt taatgtaaga agtacaaccg tcgcagatat gtttaaacaa aatatattag
     2401 aacctgttgt aattaaagaa caaataatca aatctgctac cgaagcagcc tgtatgcttt
     2461 tacgaattga tgatgttatt gcatcaagca aatcaaaagg aggcccacct ggaggcggta
     2521 tgcccggtgg catgggcggc atgggcggca tgggcggcat gggcggcatg cctggaatgg
     2581 atatggaata attttccgtt ctatctttct ctttttaata atagatgaat atttcggtta
     2641 actacgataa gatgaaaaaa ggtaactcaa atttattgag tgttaaaata acgcattgtt
     2701 atttttagta tttctaaaaa gattaacgaa tctgtaatca tgtagagaat ttatcaagta
     2761 tcatatcaga ccattttctt ccactacttt ttaggatata acagtcaata ctagataata
     2821 atttctccat catttttttc tccgcctcta aaagatcata tgctgtttta ccctcaattc
     2881 gtaaatattt tattaaatca ctataaggtg gaaatagaaa tgcgtcatca ctattttcta
     2941 tagctctttt taatgcatca tctaccgaaa tcctgatcat tttttcttct ttttcccctg
     3001 cactaccttt ttccaaaaag aaaagagaat ttattgttgt cttatcttta atttttgcat
     3061 cttttataat gtcgctaatg tgatacttag gaggttttat tcttgactgt ccaattgtat
     3121 taatcgtaaa gatcggtaca ttatgatctg ccaatttatg catcaaactc ctgccagttt
     3181 ttgagtgaat caaacttcta attctcattc cttttttatt atttgtatca ttatcactat
     3241 tatgagatga tgattgtgcc tcaaccgctg ttctatatgt atgtgcacta attgtcattg
     3301 attttggaaa gcatactgca gtgtttggca gattgattat agtcatatca tcagacaaaa
     3361 aataaaatcc atgtttaaca cattttatta cagttgttgt cttgccagtg tctggaggcg
     3421 ctgaaaggaa aaatccttga ccagttggag aggacataca cgcagaatgt aaaagaatgt
     3481 attctttaga aagaaagaga aatctgagaa taggttctac taaattcaca tatgtaacat
     3541 gtttggatct tgatattaat tcattaatgg atacctctat tttgcttcca ttaaatttta
     3601 tttggaattg agctcctgaa gaaccaaaat gttctgtata tttgatagta tattgtgaac
     3661 ctccagattc aaagttatct gtcattaatt tcctagtagc acgaaatttg gacatatcgg
     3721 aatctttgga aaaagatctt atcagtagtg tgatgtcagg atcactaaac tcttttgacg
     3781 aaaaatattc taactcttgt aaatttatct ctgacttaac cttaatgata tcatgaatat
     3841 tatagtaata caaatataca aaattactat ttttaaaaga gatttaaaca ctattccttt
     3901 atcttatata agaatgttca attattatat cttttgagat tttgaaatgg ttttagtatc
     3961 gtatatttat tataacatta tatttgtgta tattaagaat attatgaact atattttaat
     4021 agtaattaaa tatagtataa aggtataaga aatttattac taaaaagaat attaaaaaat
     4081 gttataaata taactcacga taattttaat atgcagatgt ttcctatcaa aaacaagtgc
     4141 aaaccaatca tcacgaaata tttgaaacta aaaatatgta tacaatttca ataataattc
     4201 ctacatataa cgaagcagaa aatattatat cattattaga taaaattaaa gaaaatttaa
     4261 ctcacaatgt ttttacagaa attataatag tagatgataa ttctccagat ggtactagta
     4321 atatcgtaca aaattacata gattacgatg ttgaatctaa aagaggaaat gcaaacttta
     4381 aagaaaagtt atattcactt aaaattatta atagaaaaat caaaaatggt ttaatcccag
     4441 ccatattaga tggagtcaaa tcatcccaag gtgattatgt actggttatg gatgcagatt
     4501 tttctcatcc tcctgaaatg ataccaaaaa ttatagagaa atctgtaggc aattctaatt
     4561 ctatagttat agcatctcgt tatacaaaga atggatctat agttggatgg ccatttaaaa
     4621 gacgactgat aagtaaagct gcagccgtta ttgcgaaatt tggtctcaat gtaaataatg
     4681 ttacagatcc aatgtcggga ttttttgccg tacctagaca tattatcgaa aacctaaaaa
     4741 ttgatacaaa gggttataag atcctactag aaataattgt aaaatctaaa gatattccaa
     4801 tatttgagat accatatact ttcatggaca gaaaatcagg taaaagcaaa atgggcttta
     4861 gtgttatttt tgactatata ggctctgtat ggcagttata tagatatgga cgaaatcacc
     4921 aaaagaaata aatttgttgc ttcttgcatt atgaatacaa agataattat tagaatccaa
     4981 accccttcta gttaaatctt tgtaatatac tgggtgatta attttgaatc aaatgactaa
     5041 aagtagcaat cttgggaata aaaaaatact tttaattttc atacctctag ttttaagttc
     5101 ttttacaagt ctatggaata taacaggttt tcctagccta catgtcgatg agggtcacta
     5161 tatgcgtaaa gctatgtcta cactggaagg agaaggt
//
LOCUS       DATAFW010000108         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2878899, whole genome shotgun sequence.
ACCESSION   DATAFW010000108 DATAFW010000000
VERSION     DATAFW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2878899"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /gene="metK"
                     /locus_tag="VHJ57_02570"
     CDS             <1..659
                     /gene="metK"
                     /locus_tag="VHJ57_02570"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX2487449.1"
                     /translation="RPDGKSQVSVVYEDCIPKRIDTVVLSTQHSPEIELGQLREEIIN
                     KVIRPTCAEWINSQTRFIINPTGRFVLGGPSADTGLTGRKIIIDTYGGMGRHGGGAFS
                     GKDPSKVDRSACYMARYIAKNIVAAELARKCEVQIAYAIGVAEPVSVMVDTFGTGRIE
                     EEQIVNRVKNTFDMRPSKIIDTLSLKRPIYKKTAAYGHFGRNDPSFSWEKTDRTHLLI
                     "
     gene            complement(716..1591)
                     /locus_tag="VHJ57_02575"
     CDS             complement(716..1591)
                     /locus_tag="VHJ57_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX2487450.1"
                     /translation="MPSLKDSARVVKQLETLEYRVLKVFVSSIKHHEIINNHNILSYS
                     NLHKDRVEYALENLLKLKLIFKTEKGFKLLTAGLDVFALKLLVESGIIIGIGNSLGIG
                     KESDVVEAISEIHQRRAVKFFRIGRISFTDTKRKRSLEKNKNVNNWLLINIEAAKREY
                     DFLIKLKSTKMNIATPYFRSMHSIVMDRINGLRLIEIRNLHNPKEIFYKVFEQIRLAY
                     NENIINGDLSEYNILLDENNDILIIDWPQAVTLDHPNAEFLIRRDLHNVIRYFRRKFD
                     LSIDEDSYFNSLVKK"
     gene            1773..2969
                     /locus_tag="VHJ57_02580"
     CDS             1773..2969
                     /locus_tag="VHJ57_02580"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HEX2487451.1"
                     /translation="MSHESNLKVDKSTAEDEDLKLAEIEGEAELSLEIIEKRRKGLLR
                     TGFTTGSSATAATKAALLALISSQIYNTIEIALPKGKIVKLKIAWTRYSPSTQSVTSA
                     VIKDAGDDPDVTHGAEICSTVSLTTEKGRVEIDGGLGVGRVTRPGLGLEIGSAAINPV
                     PKKMIEQMIFDSAKAILTHKGIKVIISVPDGIELAKKTDNPRLGIIGGISILGTTGIV
                     FPYSTASFAASIRQSLDVAISLGTDVVVLTTGGRSEDYMKNLYKYLPDFAFVQMGDFS
                     GYSIKQCSNRNIKKIIIAGFIGKLTKMAMGIKQTHVRGSHVSMDYMANLASLCTTSKN
                     IINDIRKANTARHVSEIIIQNNVENFFDLLCERVYLEMYNHSNKLMEIEVVMFGFDGK
                     VLGKYP"
     gene            3126..4193
                     /locus_tag="VHJ57_02585"
     CDS             3126..4193
                     /locus_tag="VHJ57_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HEX2487452.1"
                     /translation="MKDKIAVIAITKNGIRIAGVIKKYITNAEIYVPNKFYNEDSTII
                     WFKEPTQKKISDLFNSYDALICIFSLGAVVRLISNLLKDKKTDPAILVIDDKATFVIS
                     ALSGHLGGANALSRYIADSLKSIPVITTAADVNKTISVDLIGNELGWIIDNYENVTKV
                     SAHMVNEEKIAIFQDVGERTWWDKENLPSNVVMVKDLENIKDNLYKGGIIITDKIITD
                     NVIRSKSVIYRPKTLVIGIGIHWDTSKDDILKGVKAIFEKNGLSFASIKSLSSIKKKI
                     RVKGLDEFSKELQIPLFLFEKEELEKISVPNPSDTVKKFEGITSVSEASSILHSRGTL
                     IIPKQKFPPNLTIAVCRVNFA"
     gene            4190..4945
                     /locus_tag="VHJ57_02590"
     CDS             4190..4945
                     /locus_tag="VHJ57_02590"
                     /inference="COORDINATES: protein motif:HMM:NF017944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX2487453.1"
                     /translation="MRKALLIIDRGSRELEVQDELFKIAKDAKEKGGYIYSNFCFLEV
                     IPPYIEEGIRNCIQENVDLITVMPYFLYPGMKLKDAVQKTAAISKEKGIKLVITKPLC
                     YSEVLKFVIMDRLTKLKSDNDIEIKDNECDVLVIGHGSSDRRATEAFIFTINAIKHSY
                     RNVDYCFLELARPTIQDGINCIIKKNPRILLIMPYFLHKGIHIKQDVINELKNALKGH
                     SFSNIFIADHIGVDDKMVNLILHRANEVEKKVV"
     gene            4926..5615
                     /locus_tag="VHJ57_02595"
     CDS             4926..5615
                     /locus_tag="VHJ57_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HEX2487454.1"
                     /translation="MKKRLFKKEITENPSNRSMSLKSFDIEKSSFDIIENEIGKYRYK
                     DELEWSIVRRVIHATADFDFARQQKLLFNNEAITSGFQAIETKCNIITDVEMVRAGIS
                     RNSLTKLDLKCLCNISDSNIIDESKRLNKTRAETSMRNLAKEMNGGIIVIGNAPTALN
                     EVIKMINEKITIPSLIIGVPVGFVSALESKIELSKSGIPHITNIGRKGGSSVASSIVN
                     ALMLLYLHKNN"
     gene            5629..6120
                     /locus_tag="VHJ57_02600"
     CDS             5629..6120
                     /locus_tag="VHJ57_02600"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX2487455.1"
                     /translation="MLEIEMQVNLKSYAYINLILKELVTEIEINSTPEKIWKVITDFK
                     NYYQWNPILQKINGEAEIGNKLEIHLQTIGGKNRVYHPKVIKITPNYELRWKGTFLLP
                     QIFSGERIFLIEKLSENKVNFVNKEIFSGIGVNFTTQKMEDDIIASFKTMNEALKKTI
                     EKS"
     gene            6277..6561
                     /locus_tag="VHJ57_02605"
     CDS             6277..6561
                     /locus_tag="VHJ57_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX2487456.1"
                     /translation="MKAIWNDVILAESNSTVVIEGNHYFPPESIKRQFFKDSSTHTRC
                     GWKGIASYYTIKIEDELNPDSAWYYPEPLDKARHIKNYVAFWNGIKVEED"
     gene            complement(6610..>7158)
                     /locus_tag="VHJ57_02610"
     CDS             complement(6610..>7158)
                     /locus_tag="VHJ57_02610"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX2487457.1"
                     /translation="VKGDSVLIFGIGPIGILHAMLAKIYGASTIYCIDINQNRVDFCK
                     KMNLGICINGRISEIDSLFDLNDRFSPDLIIIATSDMSVLNKSVDLIRKGGTILIFGE
                     PENEIKVNVDINKIYSKEVKIKTTYAATNEEIHEALVMITHMDIDVNKIITHRYSISE
                     SKDAFKKVHDRKDVIKAIIINK"
BASE COUNT         2602 a         1013 c         1182 g         2361 t
ORIGIN      
        1 taaggccaga tggaaaatct caggtttctg tagtatatga agattgtatt cctaaacgaa
       61 ttgatacagt agttctctct actcaacatt ctcctgaaat agaactaggt cagttacggg
      121 aggaaataat caacaaggtt atcagaccca cctgtgctga atggattaat agtcaaacca
      181 gatttatcat taatccaaca ggaaggtttg tcttaggagg tccttcggca gatactggtc
      241 ttacaggaag aaagataatt atagatacat atggtggtat ggggcgtcat ggaggcggtg
      301 ctttttctgg caaagatcct tcaaaggtag atcgctctgc atgctatatg gcaagatata
      361 ttgcaaaaaa tattgtggcc gcagagttag ctcgaaaatg tgaagtacaa atcgcatatg
      421 caatcggagt ggctgaacct gtttcagtta tggttgatac atttggcaca ggtagaattg
      481 aagaagagca aattgtaaat agggtaaaaa acaccttcga tatgcgtccc tcgaaaataa
      541 tagacacctt atcattaaaa agacctatat ataaaaaaac tgctgcctat ggtcattttg
      601 ggaggaacga tccctcattt tcttgggaaa aaactgatag aacgcatttg ttaatataat
      661 aagtttaata ttttttacat tgttagaaga atgtaagatt gtagagattt aattattatt
      721 ttttgaccaa actattaaaa tatgaatctt catctatgct taagtcaaat tttcgtctaa
      781 aataccgtat tacattatgt aaatctcttc gaataaggaa ttctgcattt ggatggtcta
      841 aagtaactgc ttgaggccag tcgattatca aaatatcatt attttcgtcc aaaagaatat
      901 tatattcact taaatcgcca ttaatgatat tttcgttata cgcgagtcgt atttgttcga
      961 atactttata aaaaatttct ttaggattat gtaaatttct tatttctatt aaacgtaatc
     1021 catttatcct atccattact atggaatgca tagatctgaa gtaaggtgta gcaatattca
     1081 tttttgtcga ttttaatttg attaaaaaat catattctct tttagctgct tcgatattta
     1141 ttaataacca gttgttgaca tttttatttt tttcaagaga cctttttcgc ttagtatctg
     1201 taaaacttat tctacctatt ctaaagaatt taacagctct tcgttggtga atttctgata
     1261 tagcttctac tacatcagat tctttgccaa ttcccaatga attgccaatt cctattatta
     1321 ttcctgactc caccaagagt ttcaaagcaa atacgtctaa ccctgctgta agtaatttaa
     1381 aacctttttc tgttttaaat attaatttta attttaataa attttctagc gcatactcca
     1441 ctcgatcttt atgtaaattc gaataagaaa gaatgttatg attatttata atttcatgat
     1501 gtttaataga agaaacaaat actttcagaa ctctatattc cagggtctct aattgtttta
     1561 ctacacgtgc agaatctttt agtgaaggca attcttttca caatatctta attatctgtg
     1621 aaatttcatc atctttatat aaagatttca aatttgtttc ttttgttaga atataattgc
     1681 atactttatt attagtaaat tactgtaata ttttaaatag acaaaattta tagcataaat
     1741 ataaaataat gttagcaatc cgaggagata atatgagtca tgaatctaat ctgaaagtcg
     1801 ataaaagcac cgctgaggat gaggatctta aattggcaga aatcgaagga gaagcagagc
     1861 tttcattaga aatcatagaa aaacgtagaa aaggactctt aagaacggga tttacaacag
     1921 gttcttctgc tactgcggcg actaaagcag cgttattagc actaatttca tcccaaatat
     1981 ataatacaat agaaattgcc ctcccaaaag gtaaaattgt aaaactaaaa attgcatgga
     2041 caagatattc tccaagtaca caatcagtaa ctagtgcagt aataaaagat gctggtgatg
     2101 atcctgacgt aacacatgga gctgaaatat gctctaccgt atctttgacc actgaaaaag
     2161 gtcgcgttga gatagacggc ggactaggag taggacgggt tactcgtcca ggacttggat
     2221 tggaaatagg tagtgctgca attaatcctg ttccaaaaaa aatgatagaa caaatgattt
     2281 tcgattcagc aaaagctata ctcacacaca aaggcattaa ggttattatt tctgtgccag
     2341 acggcatcga attagcaaaa aaaacagata atccaagatt aggtataata ggaggaattt
     2401 caattttagg aacgacagga atagtatttc cttattctac cgcttcattt gcagcatcta
     2461 ttagacaaag tttagatgtt gccatatcac tcggcacaga tgtagtggta ttgacaacgg
     2521 gtggaagaag tgaagattat atgaaaaatc tttacaaata tcttcccgat tttgccttcg
     2581 ttcaaatggg tgatttctct ggatattcaa ttaaacagtg ttcaaataga aacataaaaa
     2641 aaattattat agctggattt atcggaaagt tgactaaaat ggctatggga ataaaacaaa
     2701 ctcatgtacg gggttcccat gttagtatgg attatatggc taatttagca tctttgtgta
     2761 caacttcaaa aaatatcatt aacgacataa ggaaagccaa taccgcaaga catgtatctg
     2821 aaataataat tcaaaacaat gtcgaaaatt ttttcgattt actgtgcgaa agagtttatc
     2881 tagaaatgta taatcattca aataaattaa tggaaattga ggttgttatg tttggttttg
     2941 acgggaaagt attaggcaaa tatccatgat aattaatttt ttaaatataa atacttaaac
     3001 agatattttc tatccaagat ttatttatta tgttttaaag attgaggata tattattagt
     3061 taatcagaaa tttatttatt aaaatatgca aagaattaat tttgactaat taataaccaa
     3121 aagagttgaa ggataaaatt gcagttatcg ccataacaaa aaacggtata agaatagctg
     3181 gagttatcaa aaaatacatt actaatgccg agatatatgt tcctaacaaa ttttataatg
     3241 aggattctac tattatttgg tttaaagaac ctactcaaaa aaaaatctca gatcttttta
     3301 attcgtatga tgctttaatt tgtatttttt ctttaggggc ggtagtaaga ttaatttcta
     3361 atcttctgaa ggataagaaa actgatcctg ctattttagt tatcgatgat aaagcaacgt
     3421 ttgttattag tgctctttct ggacatttag gtggagccaa tgctttatcc agatatattg
     3481 ctgattcttt aaagtcaata cctgtaataa ctactgctgc cgatgtaaat aaaaccatta
     3541 gtgtggattt aattggtaac gaattaggtt ggattataga taactatgag aatgttacga
     3601 aagtaagtgc acatatggtt aatgaagaaa aaatagcaat cttccaagat gtaggtgagc
     3661 gtacatggtg ggacaaagag aaccttccat ccaatgttgt tatggtcaaa gatcttgaaa
     3721 atatcaaaga taatttatat aaaggaggga taattatcac cgataaaata attacagata
     3781 atgtaattcg ttcgaaatca gtaatatata gacctaaaac tttagtaatt ggtataggca
     3841 tacattggga tacttcaaag gatgatattc taaaaggggt taaggctatt ttcgagaaaa
     3901 atggtttgag ctttgcatca atcaaaagtc tttcttctat taaaaagaaa atccgggtca
     3961 agggattgga tgaattctct aaagagcttc aaattcctct ctttcttttt gaaaaagaag
     4021 aacttgagaa aatttcggtc cctaatcctt ctgatacagt taagaaattt gagggaatta
     4081 ctagtgtttc tgaagcatca tcaatactac attcgcgagg tacattaatt atcccaaaac
     4141 aaaaatttcc tcctaattta acgatagcag tatgtagggt gaattttgca tgagaaaggc
     4201 ccttttgatt atagatagag gaagccgaga attggaagtg caagatgaac tatttaaaat
     4261 agcgaaggat gctaaagaaa aaggaggata tatatattca aatttctgtt ttctggaagt
     4321 gatacctccc tatattgaag aaggaattag aaattgtatc caggaaaatg tagatttgat
     4381 aacggttatg ccttattttc tttatcctgg aatgaaattg aaagatgccg ttcagaaaac
     4441 tgcagcaatt tctaaagaga aaggaatcaa attagttatt acaaaacctc tttgttattc
     4501 agaagttctt aaattcgtaa taatggatag attaacaaaa ttaaagtcag ataatgatat
     4561 tgaaattaaa gataacgagt gcgatgttct ggttattggt catggaagct cagatagaag
     4621 agcaacagag gcttttattt ttaccattaa tgctataaaa cactcttata gaaatgttga
     4681 ttattgtttt ctagaattag cacgaccaac tatacaagac ggtattaact gtattatcaa
     4741 aaaaaatcca agaattctct taataatgcc ttatttttta cataaaggta tacatattaa
     4801 acaagatgta attaacgaac ttaaaaatgc attaaaaggc cattcttttt ctaatatttt
     4861 tattgcagat catattggag ttgatgataa aatggtaaat cttattttgc accgagcaaa
     4921 cgaagttgaa aaaaaggttg tttaagaaag aaatcactga gaatcctagc aatagatcta
     4981 tgtctcttaa atcctttgat attgaaaaat ctagctttga cataatagag aatgaaattg
     5041 gtaaatatag gtacaaagat gaattggaat ggagtattgt tagacgagta attcatgcta
     5101 ctgcagattt tgactttgct cgccaacaaa aacttttgtt taacaatgag gctattacct
     5161 caggatttca agcaatcgaa actaaatgta atatcattac tgatgtagaa atggtcagag
     5221 caggaatatc acgaaactca ctcactaaac ttgacttaaa gtgtctttgt aacatctctg
     5281 atagtaatat catcgatgaa tctaagagac ttaataaaac acgggcagaa acctctatgc
     5341 gtaacttggc aaaagagatg aatggaggaa taattgtaat agggaatgca ccaactgcac
     5401 taaatgaagt cattaaaatg attaatgaga agataacaat tccatcttta ataattggtg
     5461 tgcctgtagg ctttgtttcc gcactcgaat caaaaataga actctctaaa agcggtatcc
     5521 ctcatatcac taatataggc cgaaaaggag gaagttctgt agcatcctca atagtaaatg
     5581 ccttaatgtt attgtatctt cataaaaata attaatataa taaattgttt gcttgaaatt
     5641 gaaatgcaag taaatcttaa aagctatgct tatattaatt taatattgaa agaactagtt
     5701 actgaaatag agatcaattc aacccctgaa aaaatatgga aggtaattac cgattttaaa
     5761 aattattatc aatggaatcc tatacttcaa aaaattaatg gagaagctga aattggaaat
     5821 aaattagaaa ttcatttaca aacgatagga ggaaagaata gagtatatca ccctaaagtt
     5881 attaaaatca ctcctaacta tgaattaaga tggaaaggta cattcctcct accacaaata
     5941 ttttcaggtg aaagaatttt tttaattgaa aaactttctg aaaacaaggt caattttgta
     6001 aataaggaaa tattttcagg aatcggggtc aactttacta cccaaaaaat ggaagatgat
     6061 attattgcaa gttttaaaac aatgaacgaa gcattaaaaa aaaccataga gaaatcataa
     6121 aatatacaga aatcttatga aattaatttc ttattaatta atcaattaat taattaatta
     6181 tgattaattg gacatgtaat tcatctatca agatatgcta tatagtaaag gtttagtata
     6241 gcaatgtttt aaagtagaat aatcttaaaa ttaaatatga aagcaatttg gaacgatgta
     6301 attttggcag aaagtaattc gacggtagta atcgaaggaa accattactt ccctcctgaa
     6361 tctattaaaa gacaattttt taaagatagt agcacgcata caagatgcgg ttggaaagga
     6421 attgcaagct attatacaat aaagatcgaa gatgaactta atccagattc agcatggtat
     6481 tatccagaac ctttagacaa agctagacat ataaaaaatt atgtggcttt ttggaatgga
     6541 ataaaagtag aagaagatta aataagatca ttttatattt tattttgtta tatggattta
     6601 cttagatcat cacttattta ttatgatagc ttttattaca tcttttctat catgtacttt
     6661 tttaaaggca tccttagatt ctgatatact atatctgtgt gttattattt tattaacatc
     6721 aatgtccata tgtgttatca taactaacgc ttcatgtatt tcctcattag tagcagcgta
     6781 tgttgtttta attttgactt cttttgagta aattttatta atatctacat ttactttaat
     6841 ttcattttca ggttccccaa aaattaagat ggttcctcct tttcttatga gatcaactga
     6901 tttatttaaa acactcatat cagatgtagc aataatgatt aaatctggag aaaacctatc
     6961 attcaaatca aatagactgt caatttctga tattcttcca ttgatgcata ttcctagatt
     7021 cattttttta caaaagtcaa ccctattttg atttatgtct atacaataaa ttgtggaagc
     7081 cccataaatt ttagctaaca tagcatgaag aataccgata ggaccaatcc caaatattaa
     7141 tactgaatca ccttttac
//
LOCUS       DATAFW010000109         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5171268, whole genome shotgun sequence.
ACCESSION   DATAFW010000109 DATAFW010000000
VERSION     DATAFW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5171268"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VHJ57_02615"
     CDS             <1..1124
                     /locus_tag="VHJ57_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX2487458.1"
                     /translation="KGFASNEGTKKFRDNAIKKGKSYLHFKEFDDLILSSIGMGTYLG
                     DLSKEDDIDIENAFYESVKSHAINVIDSAINYRAMKSEKSIGRSITRLVNDRIISRDE
                     IFVSTKNGYITNDGDYPMLDVWEYIQRMYISTGIIKAEDISSGYNVLNPAYIEKCIER
                     SRFNLKLDTLDLVYIHNAFESWNQDVSKDKFFDMLSKVFEIYEKFRSKNKIRYYGMAT
                     WTCFRVGEENKEYLSLEEVCNIAENIGGADHGFRFIQLPYNLAYSEALFLKNQNVGNE
                     KNLTILEAAERLKIGVFSSVPLLQGKLIETKIPDYSEGLTDPIMKLLQIIRSSPSIIA
                     PLIGQKKMDHVSNNNKISEIPPLSEEEFKLAIEVLTKGN"
     gene            complement(1171..1692)
                     /locus_tag="VHJ57_02620"
     CDS             complement(1171..1692)
                     /locus_tag="VHJ57_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487459.1"
                     /translation="MTILSKENAKARLDEFRILLKYMPFLQKNGFSDCGRGQIMGQMS
                     LIQEFLSESQTNNEILNLQYKDPVLTNAPNLNNPSNSMLDTPQNYTSTNTETNKQGTD
                     GSKDNTFDLDSIILENDNSSEGNSKLQTSRWLRPFKNDDPIKKKLFDNFEKNEITQSW
                     KEKIRKMTQSDII"
     gene            1855..>2148
                     /locus_tag="VHJ57_02625"
     CDS             1855..>2148
                     /locus_tag="VHJ57_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX2487460.1"
                     /translation="MDNKKYVLDTSVIIDKEISKMLQTGKIVVENSEIIIPRAVLDEL
                     QAQASTSRDQGLVGLQEIYEIRDVCNKKGIKIKFSGEKPDYDDILLAKHGRIDS"
BASE COUNT          814 a          254 c          360 g          720 t
ORIGIN      
        1 taaaaggatt tgctagcaac gaaggaacta aaaaatttag agacaatgca attaaaaaag
       61 gtaaatcata tttacacttt aaagaatttg atgacctaat attatcaagt attggaatgg
      121 gtacatattt aggtgatctc tctaaagaag atgatataga tattgaaaat gcattttatg
      181 aatcagtaaa atcacatgca attaatgtaa ttgatagtgc aataaattat agagcaatga
      241 aatcagaaaa gagcattggc agatcaataa caagacttgt aaatgacaga ataatttcta
      301 gagatgagat ttttgtaagt acaaagaatg gatatataac aaatgacgga gactatccaa
      361 tgcttgatgt ttgggaatat atacaaagaa tgtatatatc tacaggaatc ataaaagcag
      421 aagatataag ttctggatat aatgttctta atccagccta tatagagaaa tgtatagaga
      481 gatcacggtt taacttaaaa cttgatacac tagatctggt atatatccat aatgcttttg
      541 aaagttggaa tcaggatgtt agtaaggata aattttttga catgctttct aaagtttttg
      601 aaatttatga aaaattccgt tctaaaaata aaattagata ttatggaatg gcgacttgga
      661 catgttttag agtaggtgaa gaaaataaag aatatttatc acttgaagaa gtgtgtaata
      721 tcgctgaaaa tataggaggc gcagatcatg gatttcgttt tatccagctt ccatacaatt
      781 tagcatatag tgaagcgtta tttttgaaaa atcaaaatgt aggaaatgaa aaaaacctta
      841 caattttaga agctgcagaa agattaaaaa taggagtatt ttctagtgtt ccattacttc
      901 agggaaaatt aatagaaact aagattcctg actattcgga aggattaaca gatccgatta
      961 tgaaactact ccaaataata agatcaagtc cttctataat agcccctttg ataggtcaga
     1021 aaaaaatgga tcatgtaagt aataataata aaatttccga aatcccacca ctaagtgaag
     1081 aagaattcaa attagctatt gaggtactca ctaaaggaaa ttaacaatta ttacaattat
     1141 tgagatagta taaaaataaa attttatagt ttatataata tctgattggg tcatttttct
     1201 tattttttct ttccatgact gtgtaatttc attcttttcg aaattatcaa acaatttctt
     1261 ttttatggga tcatcatttt taaatggtcg taaccaacga gaggtttgta atttactatt
     1321 gccttcagaa gaattatcgt tctctaatat tatactatcg aggtcaaaag tattatcttt
     1381 tgaaccatca gtaccttgtt tgtttgtttc agtattggta gatgtataat tttgtggtgt
     1441 atctaacatt gaattagaag gattattaag atttggtgca tttgttagaa caggatcctt
     1501 atactgcaaa tttaaaattt cattattagt ttgcgactct gaaagaaatt cttgtattaa
     1561 cgacatttga cccataattt gacctctacc acaatcagaa aagccgttct tttgtagaaa
     1621 aggcatatac ttgagcaata ttctaaattc atccaatcga gctttggcat tttccttaga
     1681 taatatcgtc atttcattgt aaatggtttg attcttttaa attaaggctg gttcaattat
     1741 tttgctaatt aacattctaa aagcgagcta gctcatttgg ggatcaagag atatgaagtt
     1801 atagaattaa aatatattta agtacgaata aagatttaca attattaaag taatttggat
     1861 aacaaaaaat atgttttaga tactagtgtt ataattgata aagaaatctc taaaatgtta
     1921 cagactggaa aaatcgtagt tgaaaatagt gaaataatta ttcctagggc agttttagat
     1981 gaattacaag ctcaggcatc cactagcaga gatcaaggtt tagtaggtct ccaagaaatt
     2041 tatgaaatac gagatgtttg taataaaaaa ggtattaaaa taaaattttc tggtgaaaaa
     2101 cctgattatg atgatatatt attagcaaag catggaagaa ttgattca
//
LOCUS       DATAFW010000110         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_238974, whole genome shotgun sequence.
ACCESSION   DATAFW010000110 DATAFW010000000
VERSION     DATAFW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238974"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..693
                     /locus_tag="VHJ57_02630"
     CDS             349..693
                     /locus_tag="VHJ57_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487461.1"
                     /translation="MIRYNGSKLSISAIIVGLVLAGFTLFSTQVFAAEPSPSASEGLT
                     KPLLAIAAAIAIAGGLIGTGNAQQGIGAAGMGIIAEKPEKFGQVLFFFVIPETLWIIG
                     FVLGIILLLGIL"
     gene            715..1278
                     /locus_tag="VHJ57_02635"
     CDS             715..1278
                     /locus_tag="VHJ57_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HEX2487462.1"
                     /translation="MSLDTFIQEIEERKTNKINLLDSTLSEKIARIQHTKESTLKEMQ
                     QHYTDEANTKSQKEAARIIEATRLKAKKILFDAINASMDSTFNTIREELKKYSRKPDY
                     KITLENMVKYAKKKLGSQDIVIHCRNDDTMILKEMNITPGSSIQTIGGILAENKSETM
                     ELDLTFEELLRTHEDKIQNFLLERLMK"
     gene            1281..2357
                     /locus_tag="VHJ57_02640"
     CDS             1281..2357
                     /locus_tag="VHJ57_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEX2487463.1"
                     /translation="MATSSYSSSFGRLKSLSINFISPIVLQNLVHAKDVAEMTRILEP
                     TWYGPEIERAAALYSPPELLEVALNRHIVEINKIALESTPFSGRDAIRAYLLKWDIHN
                     IELILSSKIIGRAITETEPFLVSSRNFPAGISAGNISHDEMKIILSQAGVDGVVNHLV
                     KYRYGPILMQHLDTYQKTGDLGPMMADLIAYYYTTLIESLRFFQGDEGVIRDLFRVQI
                     DKRNILSLLKGKDSNLERELINKHLVKGGNISRDELFDIFGSKSIEEFVGQVQKHYNL
                     ADLLKETYMKTHSLIDFEVAFDKFIGKSYLKRLKNISLSLGSIFHFIIAAEYEWDNIK
                     RIAYGKRYDLSLERITSMLILETE"
     gene            2359..2703
                     /locus_tag="VHJ57_02645"
     CDS             2359..2703
                     /locus_tag="VHJ57_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEX2487464.1"
                     /translation="MSSKQQHTESKSRIAVLGDRELVIGYRLLGVDDTFIVNGGDESF
                     KTMESLFFSHKYSMIIASHFIRDYLPSIFRKKVEASIEPLVLFMPLLKGNIQEESISS
                     LARRVLGINIKY"
     gene            2732..>3868
                     /locus_tag="VHJ57_02650"
     CDS             2732..>3868
                     /locus_tag="VHJ57_02650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEX2487465.1"
                     /translation="MSTAIVSRISGPVVVATDIEQAQMYDVVRIGELGLLGEIIRLEG
                     NKATIQVYEDTTGLRPGEKIVNTRRPLSIQLGPGLLTSIYDGIQRPLDILRQESGDFI
                     SRGKIIPALDQHRKWEFVPIKKKGEDVSQGEIIGEVQETPLIRHKIVVPFGTRGQLSE
                     IAEGNYTVSENVAQIKTNSDIIDVGLSNWWMVRKPRPVLNKLPPESPLLTGQRVLDTF
                     FPLAKGGTAAIPGPFGSGKTVTQQQLAKWADSNIIVYVGCGERGNEMTEILTTFPELE
                     DPKSKRPLMERTILVANTSNMPVAAREASIYTGITMGEYYRDMGYDVALMADSTSRWA
                     EALREISGRLEEMPGEEGYPAYLGSRLAEFYERGGKAVVVSPEER"
BASE COUNT         1368 a          593 c          745 g         1162 t
ORIGIN      
        1 gaatttttac tatataagaa aataactaat ttgtaacctg atgtgtttag aataattatt
       61 gtatattata atatacatat atacaattca ttataataat aatcataatc ataataatca
      121 taataataat tttttataaa actacttttt tgatacatat tataaagatt atttattttg
      181 aaaagctcaa gttatttatt atttatatac atcctcaaat tataccactt taggtgcaaa
      241 ttattccata tatcacaaga actttagcat tctaatgaaa aattcaatcg agatttacta
      301 ttaagaaaac tcttataaat ctctatttat aataatcctt agctaataat gatacgatat
      361 aatggttcaa aattgtctat tagtgcaata attgtgggac tcgtcctagc tggatttaca
      421 ttattttcta ctcaagtatt cgcagctgaa ccctcgccgt ctgcaagtga gggactaaca
      481 aagccactat tggcaatagc tgcagcaatc gcaatagctg gaggactaat aggtactggc
      541 aatgcacagc agggtattgg tgctgcagga atgggaataa tagcagagaa gcccgaaaag
      601 tttggacagg tactcttctt ctttgtcata cctgaaactc tttggatcat cggttttgta
      661 ctaggaatca ttctcttact tggtatatta taacagtagg agtacagatg aattatgagc
      721 ttggatacat tcatccaaga aatcgaggaa agaaaaacaa ataaaattaa tctgcttgat
      781 agtacattgt ctgaaaaaat agcacggatt caacatacca aggaaagtac actcaaagaa
      841 atgcaacaac attatacgga tgaggctaat acaaaatccc aaaaggaagc agctagaata
      901 attgaggcaa ctaggttaaa ggcaaaaaaa atcctctttg acgcaattaa tgcaagtatg
      961 gattctacat ttaatacaat aagagaggaa ttaaaaaaat atagtagaaa accagattat
     1021 aaaattacct tagaaaatat ggtcaagtat gctaagaaga aattgggtag tcaggatatc
     1081 gtaatacact gccggaatga tgatactatg attttaaaag aaatgaacat aaccccaggt
     1141 tcatcaattc aaactattgg gggaatttta gcagaaaaca agtcagagac aatggagctt
     1201 gatttaactt ttgaagagct attaagaact catgaagata aaattcagaa ttttctttta
     1261 gagagattga tgaagtaaag atggctacat catcgtattc atcttcgttt ggccgcttga
     1321 aatctctttc aatcaatttt atttctccaa ttgttcttca aaatttagta catgctaaag
     1381 atgttgcaga aatgacacgg atcctggaac ctacttggta tggtcccgaa atcgaaagag
     1441 cggcagctct ttattcaccc cctgagcttt tagaagtggc acttaacaga catattgttg
     1501 aaattaacaa aattgctctt gaatccactc cctttagcgg tagagatgca attagagctt
     1561 atttactaaa atgggatatt cataatattg aattaattct ctcatcaaag ataataggcc
     1621 gtgctattac cgaaactgaa cctttccttg tatcaagtcg aaattttcca gctgggattt
     1681 ccgcaggaaa tatatctcac gatgaaatga aaattatttt atctcaggct ggggtagatg
     1741 gtgtggtaaa tcatcttgtc aagtatagat atggacccat attgatgcaa catcttgata
     1801 cttatcaaaa aaccggagat cttggaccaa tgatggccga tcttatagca tattattata
     1861 ctacacttat tgagtcatta aggttttttc agggtgatga aggagtcatc agagatctat
     1921 ttagggttca aatagataaa aggaatatat taagcctctt aaagggaaag gattctaatt
     1981 tagagcggga attaattaat aaacaccttg taaaaggagg aaatatttct agagatgaat
     2041 tatttgatat atttggatca aagagtattg aagagtttgt gggtcaagta caaaagcatt
     2101 ataatttagc tgacctcctt aaggaaacat atatgaaaac tcatagtctc attgattttg
     2161 aagtcgcttt tgataaattc ataggtaaat cgtatttaaa aaggttaaaa aatatttcac
     2221 tttctctagg atccatcttt catttcatta ttgctgcaga gtacgaatgg gataacataa
     2281 agagaatagc ttatggaaag cgttatgatc tttcgttaga gcgtataact tctatgctga
     2341 ttttggagac agaataatat gtcctctaaa caacaacata cagaatcaaa aagtagaata
     2401 gctgttctag gagatcgtga acttgtaatt ggttatcgac ttttaggagt agatgataca
     2461 ttcatagtta atggaggaga tgaatccttt aaaacaatgg aaagtctttt cttttcacat
     2521 aaatattcaa tgattattgc aagccacttt attcgggatt acctcccatc aatatttagg
     2581 aaaaaagttg aagcatcaat agaaccattg gttttattta tgcctttatt aaagggaaat
     2641 atacaggaag aatcaatatc ctccttagct agacgggtac tcggaataaa tattaaatat
     2701 taaaagggat ggtttgggag taagatagaa tatgtcaaca gccattgttt ctcgtatatc
     2761 aggtccggtt gttgtagcaa ctgatattga acaagctcaa atgtacgatg ttgtacgtat
     2821 aggagaacta ggtcttctcg gagaaattat ccgtcttgag ggtaataaag ctactattca
     2881 agtttatgag gatacaactg gtcttagacc aggagaaaag atagttaaca ccagaagacc
     2941 actttcaata caacttggcc ctggcttact gacatctatt tatgatggta tacagcgtcc
     3001 gttagatatt ttacgacaag aaagtggaga ttttattagt aggggaaaaa ttattcctgc
     3061 attagatcag cacaggaaat gggaatttgt tccgattaag aaaaagggag aagatgtttc
     3121 tcaaggtgaa ataattggcg aagttcaaga aactccatta attagacaca aaatagttgt
     3181 cccatttggt actcgtggtc aattatctga aatcgcagaa ggaaattata cagtaagtga
     3241 gaatgttgca caaataaaaa caaatagcga tattattgat gtagggttat caaactggtg
     3301 gatggtaaga aaacctcgac ctgtactgaa taaacttcca cctgaatctc cgttgctaac
     3361 cgggcaacgg gttcttgata cttttttccc cctcgcaaaa ggtggtactg cagccatccc
     3421 cggtccattt ggaagtggta agacagtaac acaacaacag ctagcaaagt gggctgatag
     3481 taacataatc gtatacgttg gatgtggaga gagaggaaat gaaatgacag aaattctcac
     3541 tacttttcct gaattagaag atcccaaatc gaaacgacca cttatggaac gaactatact
     3601 tgttgccaac actagtaata tgcccgtagc agctcgtgaa gcgagtatct atacaggtat
     3661 tactatggga gaatattatc gagatatggg atatgacgtg gccttaatgg cagatagtac
     3721 atctagatgg gcagaagctc taagagaaat ttcggggagg ttagaagaaa tgccaggtga
     3781 agaaggatat ccagcttatc ttggaagcag gttagccgaa ttttatgaaa gaggaggaaa
     3841 ggcagttgta gtttctccag aagaaaga
//
LOCUS       DATAFW010000111         4248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2529152, whole genome shotgun sequence.
ACCESSION   DATAFW010000111 DATAFW010000000
VERSION     DATAFW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529152"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VHJ57_02655"
     CDS             <1..413
                     /locus_tag="VHJ57_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487466.1"
                     /translation="NPQDSTIFADEVEEYQIIHSYQNSHSHLSTLINNALWGNFNANP
                     QDSTIFADEVEEYQIIHSYQNSHSHLSTLINNALWGNLNPNPQASVSTDHVKEQFITI
                     KEDGFNDNMIFEEPKKNAQVQTTTVPFILPVPFP"
     gene            485..559
                     /locus_tag="VHJ57_02660"
     tRNA            485..559
                     /locus_tag="VHJ57_02660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:520..522,aa:Gly,seq:ccc)
     gene            complement(681..2525)
                     /locus_tag="VHJ57_02665"
     CDS             complement(681..2525)
                     /locus_tag="VHJ57_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX2487467.1"
                     /translation="MIDLSVFKNNVSLLLIIIIIGIGSLSIISYQSASFTADEIKKIA
                     EEDIKSNARIQAYDLSRILVHSLDSINSNLKVLTSSPFLFESDIDQVQNLLYSAQSST
                     LNITDAYYWIDVNGRIVTSSKIYQTDKANEILDTQNQPFFLNPKKTLTPYYSTSITSK
                     GTPSSFLYISYPLIKQQILKYQNNTYNAQQIFNGVLVAAISVKSLGIFLQNEMTSSII
                     GNVGLMDRDGVIIYARNQSLIGNNYISNEFQSLIPNDLRESYNKIIKKGLEGKSLAED
                     LTYNGSTITLSYEPIVINGKYLWTLYVSFPHQLASNVGSLIAQQNNFSTLIVVITGSV
                     TFGIIYLILSWNKRLEREVNTRTGELKNTNISLMRSNELLEEANEQLKVHDKMQKEFI
                     NIAAHELRTPIMPILGEVELIEEDLDPKTKTVKVEEEQIQLIIRNAKRLDRLASDILD
                     VTKIESNSLKLDKVYFNLNDVISNSIRDIQNQVSSGDIGTKNIKILYEPIDIKVFGDK
                     ERINQVVSNLLSNALKFTDEGFIKIKIENTNNSIIVSVEDTGSGIHPEIFPRLFCKFA
                     TKSDKGTGLGLYISKNIIDAHGGRIWAQNHKSGIGAKFFFSLPLNKKK"
     gene            complement(2691..3200)
                     /locus_tag="VHJ57_02670"
     CDS             complement(2691..3200)
                     /locus_tag="VHJ57_02670"
                     /inference="COORDINATES: protein motif:HMM:NF028455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487468.1"
                     /translation="MKNLDSDIFILDSSAFYAGFYRSLYCKFYTTTSILQEIKHILKS
                     SITLDLLISSGALVIQDPTKESVDKIIYNANLLGDIKKLSQADISIISLAYQLNRILV
                     SDDYSVLNVSNFLNIKTVSLGNAGIVTIRKWKNFCKTCRKQYPSTIKECEICGNPTKR
                     KYKKLHSTV"
     gene            complement(3181..4002)
                     /locus_tag="VHJ57_02675"
     CDS             complement(3181..4002)
                     /locus_tag="VHJ57_02675"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX2487469.1"
                     /translation="MIKKIERVAIITKNRNNTAYEASDYIAKMLSRKSIKVSCIPPFE
                     TSNFKNNSSNFTSVVNEIDLIIALGGDGTTLRAFRFHPSDIPVFSINVGGTRGILSEI
                     KLDQIDYALDAILNGSYYYDERIRIQASLTNKLFPPGLNDILLFRTNLTRTPTVTVKF
                     RDGVVSQRMDGIIISTSTGSTGHSISVGGPLLHEELNCLIITPIASINKLPSIIIPPE
                     EIEIYSNNKMDLIIDGQDKFLVEEGSTIKITRFPINARFIRLTKKGFRQLEKLGF"
BASE COUNT         1397 a          646 c          632 g         1573 t
ORIGIN      
        1 ccaatcctca agattcaaca atatttgctg atgaggtaga ggaatatcaa attatacatt
       61 cataccaaaa ttcgcattct catctttcta cattgataaa caatgcttta tgggggaatt
      121 ttaatgccaa tcctcaagat tcaacaatat ttgctgatga ggtagaggaa tatcaaatta
      181 tacattcata ccaaaattcg cattctcatc tttctacatt gataaacaat gctttatggg
      241 ggaatttgaa tcccaatcct caagcttcag tctccacaga tcatgtaaaa gaacagttta
      301 ttacgattaa ggaagatggg tttaatgata acatgatatt tgaagaaccg aagaaaaatg
      361 cccaagtgca aaccaccact gttcctttta tattacctgt gccttttcca taactttctc
      421 aagtcatgtt ctgctaacaa gttcaaaaaa tatattgatg ctcttggaaa tagtattgag
      481 gtaggcgaag ttagcttagc ctggttaggg tacgagcttc ccaagctcgg ggtcgcgggt
      541 tcaaatcccg cacttcgcat atgtgctaac tatttcctat ttttataatt gattttaatc
      601 ttatattgca atatgtatct aatatttacc ttgaatgaaa agacacttta tcgtgaattt
      661 taataaataa aagaacttgg ttattttttc ttatttaagg gtagactaaa gaagaacttt
      721 gctccaattc ctgatttatg gttttgtgcc caaattcggc ctccatgagc atctataata
      781 ttttttgaaa tgtataagcc caaacctgtt cctttatcag atttcgtagc aaatttacaa
      841 aataaacgtg gaaaaatttc aggatggatt ccacttccag tatcttctac gctaacaata
      901 atactgttgt ttgtattttc tattttgatc tttataaacc cctcatcggt aaattttaat
      961 gcattactta ataaattact aactacttga ttgattcttt ctttatctcc aaatactttt
     1021 atgtcaatag gctcatacaa tatttttata tttttagttc ctatatctcc tgaagaaact
     1081 tgattttgga tgtctctaat agaattagat ataacatcat taagattaaa atagactttg
     1141 tctagcttta atgaattact ttcaatcttg gtaacatcca gaatatcaga tgctaatctg
     1201 tctaaccttt tagcattacg tatgatcaat tgaatctgtt cttcttctac tttgacggtt
     1261 ttagttttag gatcaagatc ttcttcaatt aattcgactt ctccaaggat aggcattatt
     1321 ggagttctta attcatgtgc agcgatattg ataaattctt tttgcatttt atcatgtact
     1381 tttaattgtt catttgcttc ttcaagaagc tcgtttgacc tcataaggga tatatttgta
     1441 ttttttaatt cacctgtcct tgtattgact tctctttcta atctcttatt ccatgataaa
     1501 attagataga taattccaaa agtaactgaa ccagttataa ctacaattag agtactaaaa
     1561 ttattttgtt gtgctattag ggaacctaca ttacttgcca attgatgcgg aaaacttacg
     1621 tataatgtcc atagatattt tccatttatt acaatcggtt catatgagag tgttatagtt
     1681 gatccattat aggttaaatc ttctgctaaa gattttcctt ctaaaccttt ttttataatt
     1741 ttattataac tttccctcag atcattgggt attaaagatt ggaattcatt tgatatataa
     1801 ttattaccta ttagtgactg atttcttgcg tatattataa caccatctcg atccatcaat
     1861 cctacatttc caataattga agatgtcatt tcattttgaa gaaagatacc gagtgatttt
     1921 acacttattg ctgctacaag tactccatta aatatttgtt gggcattata agtattgttt
     1981 tggtatttta atatttgttg tttaattaaa gggtaagaaa tgtataaaaa cgatgatggg
     2041 gttcctttag atgtaattga agtactataa tatggagtca gtgtcttttt aggattaagg
     2101 aaaaaaggtt gattttgagt atctaaaatt tcattagctt tatcagtttg atatatttta
     2161 ctagacgtca caattctgcc attcacatct atccagtaat atgcatctgt aatattgagt
     2221 gtagacgatt gtgcggaata aagtaggttt tgaacttgat caatatcact ttcaaaaaga
     2281 aagggggagg atgttaaaac ttttaagtta gagtttatag agtcaagact atgaacaagt
     2341 atcctagata aatcgtaggc ttgaattcta gcattggatt taatatcctc ttctgctatt
     2401 ttctttattt cgtcggctgt aaaagaagcc gattgataag atatgataga tagagaacca
     2461 attccaataa taattattat caataataat gaaacattat ttttgaaaac tgatagatct
     2521 atcaactatt gacaatatca tgtttattta agatgaataa attaaatagg aataattctt
     2581 atagtaaatt tctctatatt gttctttatt cgctagctta ttataattgt ttgttgaatt
     2641 tattcgttta ttaattaaaa ggaaacaata atcattaaag catgccaaaa ttaaactgtt
     2701 gaatgtaatt ttttatactt tctttttgtt ggattaccac atatttcgca ttctttgata
     2761 gttgatggat attgttttct acacgtttta caaaaattct tccattttct tattgttact
     2821 ataccagcat tacctaaaga aacggtctta atattgagga aattagatac gtttaaaact
     2881 gaataatcat cagatactaa aattctattc aattggtaag ctaaagatat aatagaaata
     2941 tcagcctgtg aaagtttttt tatatcaccc aaaagatttg cattataaat tattttatca
     3001 actgactctt tcgttgggtc ttgtataact aaggcacctg atgaaattaa taaatctaaa
     3061 gttatagagg attttaatat atgtttaatc tcttgtagaa tagaggtcgt tgtatagaat
     3121 ttacagtaga gagagcgata gaaacctgca taaaaagcac tagaatctaa aatgaaaata
     3181 tcagaatcca agtttttcaa gttgtctaaa gcccttcttt gttaatctta taaaacgagc
     3241 attaattgga aaccgagtta tttttatagt actaccttct tcgaccaaaa atttatcttg
     3301 gccatcaatt attaaatcca tcttgttatt gctataaatt tcaatctcct ctggaggtat
     3361 tattatagat ggtaatttat taattgaagc tattggggta attattaagc agtttaattc
     3421 ttcatgtaat aaaggtcctc ctacagaaat tgaatgacca gttgatccag ttgaagtaga
     3481 tattattatt ccatccatgc gctgactaac tacaccatct ctaaatttta ccgttacagt
     3541 aggcgttctg gtaagattag ttctaaaaag taagatatca tttaacccag gaggaaagag
     3601 tttattggtt aaagaagctt ggattctaat tctttcatca taataataac taccatttaa
     3661 aattgcatct agagcataat caatttgatc caatttaatt tcagaaagta tgcctcgagt
     3721 acctcccaca ttaatactaa aaacaggaat atcacttgga tggaatctaa aagctcgtaa
     3781 agtcgtaccg tctcctccta gggcgattat aagatcaatt tcatttacta cggaagtaaa
     3841 attagaactg ttatttttaa aatttgaggt ttcaaacggg gggatacatg ataccttaat
     3901 actttttctt gaaagcattt ttgcaatata gtcagatgct tcatatgctg tattatttcg
     3961 attcttggta ataatagcaa ctcgttcaat ttttttaatc ataaatcgta cattataaca
     4021 atattatagt cttaaatgca tttttattgt ccagttatta aaattattct ctttaacaaa
     4081 aatatagaaa atgatatgcc gtgctataat agattgaggt tcaaaaaaaa atatttaaat
     4141 tgtcttagaa ataaagcacc aacaccagat tgtaatatat tgggccgata gctcagcttg
     4201 gtggagcgtt cgactgataa tcgaaaggtc gagggttcaa ctcccttt
//
LOCUS       DATAFW010000112         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_415525, whole genome shotgun sequence.
ACCESSION   DATAFW010000112 DATAFW010000000
VERSION     DATAFW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415525"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..420)
                     /locus_tag="VHJ57_02680"
     CDS             complement(184..420)
                     /locus_tag="VHJ57_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487470.1"
                     /translation="MSMDKKTKSSSKKTNKKSTSVVSRKKATTPAKKATAKRKATEIN
                     ELEKSSENILESPVESPVESPVESLVEQTQSETP"
     gene            complement(812..2737)
                     /gene="gatE"
                     /locus_tag="VHJ57_02685"
     CDS             complement(812..2737)
                     /gene="gatE"
                     /locus_tag="VHJ57_02685"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HEX2487471.1"
                     /translation="MNHVTTDMNEIILKVGFEIHQQLSTQNKLFCNCKCLEVENYDSI
                     FLRKLRPAHSELGSYDRASIFEFTKMRTIKYHSSYIASCLVEADEEPPHEINRDAMDT
                     ALIISLALNSNIIDEINVMRKIVIDGSNTSGFQRTMLISYGGHLQVDGKKIGVQTICL
                     EEDSGKLLSDDGRFREYGLDRLGIPLIEIALEPVTGTPTEVVKIALTLGRLLRTTKRV
                     ARGLGSIRQDVNISVNNGKGVVEIKGVQRLDQLIKVIKYETIRQHGLILIAEKMNRER
                     KSNDIIIGDKIMDITNIFRDSSSKIVQKVISNKGNILVAIRVKGFKGLFGFEPYPDIR
                     IGKELGELVRFYGFGGLFHSDELPNYGITDIEVKKVNEILDIDISKDGFIILGGPREK
                     MSIAISAICDRLNSVSSGVPSETRAARIDGKTIYSRPKPGSARMYPETDITPFKIRDD
                     LLVALKEKVPKSWNETISYLAKKYQLNKNLAEKIFDSNYFEIFEVLCGLTKISPSFIA
                     SKLTEDILNLERQGYDSSILNNNILLDIFKRLDSGLIAKESVNLIMEKLMNRTTTSVD
                     ESIRVLGIKSISDEELETIIDKIIQDNILIIQEKGIKATSTLMGKCMTILRGKVDGKK
                     INDLINNKLNIILNLKK"
     gene            complement(2776..4164)
                     /gene="gatD"
                     /locus_tag="VHJ57_02690"
     CDS             complement(2776..4164)
                     /gene="gatD"
                     /locus_tag="VHJ57_02690"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HEX2487472.1"
                     /translation="MSINDDVQSILNDIKVGDIVNIVLNNNSVISGRLMPRYESSERD
                     HIVIKLPNGYNVGIMLKKIKFITKVSSFSIETEELPKKNLYNSSSLKSQYKNDKDLSQ
                     ISNLPKIALISTGGTIASKIDYRTGGVTSVLSAKDLYTSIPELSLYASIDTEILFNEY
                     SENIGPMQWHLIANKIIEKINSGNYDGIIISHGTDTMSYTAAALSFALQDLPIPVIIV
                     GAQRSSDRPSSDASSNLIASVIFATQLDYSGVFVAMHGSISDKEIYCHIGTRVRKNHT
                     SKRDAFKSIDIPPIAVVQDDRVEMQKIPSNIILHKNKNRNKKNKEFNIISKTKFDDRV
                     FLLKYYPGFNPILIENVQKLGYKVIIIEGTGLGHINRNCFSYLKEAIASGMLVFMTSQ
                     CIWGRTNLNVYDTGRDLQKIGIIPLYNMLSETAMVKAMWLLANYNDLDYIRNKMQENI
                     SNEITFITPIVE"
BASE COUNT         1415 a          673 c          511 g         1721 t
ORIGIN      
        1 tatattaaaa ttattaatca ttattatata attagatatt ttataattac agaatcaact
       61 gacctaattt gtcaaactgg ttgtatttgt aactatataa tataaaaata agaaagaata
      121 gtttgataaa ctaaatggtg actcccatac attagatata atcataataa cgtagtatta
      181 attttaagga gtttctgatt gagtttgctc tactagactc tctactggac tctctactgg
      241 actctctact ggactctcta gaatattttc tgatgatttt tctagttcat taatttctgt
      301 tgcttttctt tttgctgttg ctttctttgc tggtgttgtt gctttctttc tactaactac
      361 agatgtagat tttttatttg tttttttact actggatttt gtttttttat ccatagacat
      421 gtatataatt aaactactca ttcttatatt aatcggaaac agaataaaat aattattttt
      481 tattgaatat agtctccaat tattataaac ataaagatat aattaaagat agtattaata
      541 aaaatacggt aatttattaa aacaagaaat tagttttact tttattatct gtttaagcat
      601 ttatcatact aaatatacaa tcattaaatt tcaataaaaa aagaatttaa aatattaaaa
      661 tatgttcttt ctattcaatg ggattaatca aatatataga atatttatat tataaaataa
      721 taaattaaat tttattcctt tttaattcaa atacaaaaat tttgttttaa tttgatcaaa
      781 ttgaaagtaa atagacaata tataaaaaat ttcatttttt aaggtttaat ataatattta
      841 atttgttatt tatcagatca tttatttttt ttccatcaac tttgccacga agaatagtca
      901 tgcattttcc catcaaagta ctcgttgcct ttatcccttt ttcttgtata atcaatatat
      961 tatcttgtat aattttatca attattgttt caagttcctc atcactaatt gacttgattc
     1021 caagaaccct gattgattca tcaacacttg ttgttgtcct attcatcaac ttttccataa
     1081 tcaaattaac tgattcttta gcaataagac cagaatctag tcttttaaaa atatctaaaa
     1141 gaatattatt atttaatata ctagaatcat aaccttgtct ttctaagttt aaaatatctt
     1201 cagtgagttt tgatgctata aatgatggag agattttagt taaaccacaa agtacttcaa
     1261 atatttcaaa atagttagaa tcaaaaattt tttcagctaa atttttattt aattgatatt
     1321 tctttgctaa gtatgaaata gtttcattcc aagattttgg aactttttct tttagagcaa
     1381 caagaagatc atctcttatt ttaaatggag ttatgtctgt ttctggatac attcgtgctg
     1441 atcccggttt tggtctactg tatattgttt ttccatcaat tctagctgct ctagtttctg
     1501 atggaacccc tgagctaaca ctattgagtc tatcacatat tgcacttatt gcgattgaca
     1561 ttttttctct aggtccacca agaataataa aaccatcttt ggagatatca atatccaata
     1621 tttcattaac ctttttaact tctatatctg ttataccata attaggtagc tcatctgaat
     1681 gaaataggcc tccgaatcca taaaatctta ctagttctcc taattcttta cctattcgta
     1741 tatctggata tggctcaaat ccaaataatc ctttaaaacc tttaacacgt atagccacta
     1801 aaatattccc tttgttagaa attacttttt gtactatttt agatgatgaa tccctaaaaa
     1861 tgttagtaat atccattatt ttatctccaa ttattatatc atttgatttt ctttctctat
     1921 tcattttttc agcaattaaa attaaaccat gttgtctgat tgtttcatat tttattactt
     1981 ttattagttg atctagtctc tgaactcctt ttatttctac aacacctttt ccattattaa
     2041 cggatatgtt tacgtcctgt ctaatacttc ctagtcctct agctacacgt ttagttgtcc
     2101 tcaaaagcct tcctaaagta agagctattt ttactacttc tgttggtgtc ccagtaacag
     2161 gttcaagagc aatttcaatt agtggaattc caagtctatc gagtccgtat tctctaaacc
     2221 ttccatcatc tgataataac ttcccagaat cttcttctaa acaaatagtc tgtaccccta
     2281 tttttttccc atcaacctgt aaatgaccac cataagaaat taacattgtt ctttgaaatc
     2341 cagaggtatt tgatccgtca attactattt ttctcataac atttatctca tcaataatgt
     2401 tagaattaag agcaagagag attatcaatg cagtgtccat agcatctcta tttatttcat
     2461 gaggtggttc ttcatctgct tcaaccaaac agcttgcaat ataggaagaa tgatatttga
     2521 tcgtacgcat ttttgtaaat tcaaagatac ttgctcgatc atatgatcct agctcactgt
     2581 gggcaggacg caattttctt aaaaaaatag aatcataatt ttccacttcc aaacatttac
     2641 agttgcaaaa taatttgttt tgagtagata attgctgatg aatttcaaaa ccaactttta
     2701 agattatttc attcatatca gtagttacat gattcattga taaaattgta taattttaac
     2761 tattaaaaat tattattact ctacaatagg agttataaaa gtaatctcat ttgaaatatt
     2821 ttcctgcatt ttatttctaa tataatctaa atcattataa tttgctaaaa gccacatagc
     2881 ttttaccatg gcagtttcag aaagcatatt atatagtggt attataccaa tcttttgtag
     2941 atctctacct gtatcataga cattgaggtt tgttctaccc caaatacact gagatgtcat
     3001 aaaaactaac attccagatg ctattgcttc ttttaaataa gaaaaacaat ttcgattaat
     3061 atgacctaag cctgttcctt ctataattat tactttatat cctaattttt gcacattttc
     3121 tattaatatt ggattaaatc ctggataata ttttaatagg aaaaccctat catcaaattt
     3181 agtttttgat attatattga attctttatt ttttttgttt ctatttttgt ttttatgtaa
     3241 aattatattt gaaggaattt tttgcatttc gacccgatcg tcttgtacaa ctgcaattgg
     3301 aggaatatct attgatttga atgcatcacg tttacttgta tgatttttcc ttactcttgt
     3361 acctatatga caatatattt ctttgtcaga tatgctccca tgcatagcca caaatactcc
     3421 agaataatct aattgggttg caaaaataac cgatgcaata agattggaag acgcatcaga
     3481 agaaggtcga tcagaagatc tttgtgcgcc aactattata acaggaatag gaaggtcttg
     3541 taacgcgaaa cttaatgctg ctgcagtata actcattgta tcagtaccat gagaaattat
     3601 tataccatca tagtttccag aattaatctt ctctattatt ttattagcta ttaagtgcca
     3661 ttgcattgga ccaatatttt cactatattc attaaataaa atttcagtat caattgaagc
     3721 ataaagagaa agttctggaa tagaagtata tagatcttta gctgataaaa cagaggtgac
     3781 ccctcctgtt cgataatcta ttttactagc aattgtccca cctgtactaa ttagagctat
     3841 ttttggtaaa ttagaaattt gtgaaagatc tttatcattt ttgtattggg atttcaaaga
     3901 actagaatta tataggttct tttttggtaa ttcttctgtt tcgatagaaa atgaagaaac
     3961 tttggtgata aattttatct tttttaacat tatacctaca ttataaccat taggcagttt
     4021 aattactata tgatcccttt cactagactc atatcttggc ataagtctcc ctgagataac
     4081 agagttatta tttaaaacta tattgactat atcacctacc ttaatatcat taagaatgga
     4141 ttgaacatca tcattgattg acatagttat aacttatcat ataattataa tttaattcta
     4201 tcaaataata ttattaatac agttattgat tattgatgct gttcattata ttttttccat
     4261 agaagattta atctctctat accatttatt tcttctatta cttttgcagt ttcaaaatga
//
LOCUS       DATAFW010000113         5503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4996258, whole genome shotgun sequence.
ACCESSION   DATAFW010000113 DATAFW010000000
VERSION     DATAFW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4996258"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1305)
                     /locus_tag="VHJ57_02695"
     CDS             complement(175..1305)
                     /locus_tag="VHJ57_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487473.1"
                     /translation="MKFIVVSLFLTSLIGFLGLIIFYDLQSINSQGEITQTLANISVK
                     ITSPNEGKKIPSGELTISGISSDTQNKNCIVFVDWNDIKPFQKVRAAGPGGIEDFSEW
                     IFTYDSSYHEIIAGNNELTSKITCLDGSKPLTKWNSINVTGYPNNSSLLSDIQQDTTS
                     LESISKQLPLQTILPGSNQKNTTIPAEQNTTSSPLASVSSVEQNTTSSPLANLSESSV
                     EMFIPNESIVDDINNTNTSISVNPQSTSNLKSKQLMVDNPRIDLDTQQPPQPPEDELS
                     AENQTMVPPTENQTMVPPTENQTMVPPTENQTMVPPTDNQPLELQQPPPTLENQQQQQ
                     PPQLQPPTLDNETFGSKESPSLEEKSPTQGLFPPFQLPSLFP"
     gene            complement(1511..1648)
                     /locus_tag="VHJ57_02700"
     CDS             complement(1511..1648)
                     /locus_tag="VHJ57_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX2487474.1"
                     /translation="MDGYCVKCKSKREMQNEKKVTMKNGRPATQGICSVCGTKMFKIG
                     K"
     gene            complement(1793..2002)
                     /locus_tag="VHJ57_02705"
     CDS             complement(1793..2002)
                     /locus_tag="VHJ57_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487475.1"
                     /translation="MSAVPSKLKFRLNYKPINYDVKTGMFEVNSEKFTRYEEARANHW
                     RCDKCGNRFSTFKKLRKHKKDIHAY"
     gene            2201..2854
                     /locus_tag="VHJ57_02710"
     CDS             2201..2854
                     /locus_tag="VHJ57_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487476.1"
                     /translation="MTIENIIQKSSDISSSITPFMATKIKEILKKEHKDDTIDVYSKE
                     NVKIFDIIVNSRRELCIACVSLDQIFSFNYIEVLKSILKNNIKIKILLCDPHSDMLNH
                     IKYLSVFPNIHESLTSCLSKLCNMKIVSLSTKELSNIEIRIYKTILPHNIIIRDRDIH
                     GSGLILVEPYLFGINSQNRKIYQIYRRKNQEIFDLHFKSFLKLWETSPEYSCEWEEL"
     gene            complement(2912..3094)
                     /locus_tag="VHJ57_02715"
     CDS             complement(2912..3094)
                     /locus_tag="VHJ57_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487477.1"
                     /translation="MADVSINEDEKHLVLFALNQIAEMIEEGDIKEEDFNLTGYNFIN
                     LSSIYELKDRLEAAIM"
     gene            3198..3935
                     /locus_tag="VHJ57_02720"
     CDS             3198..3935
                     /locus_tag="VHJ57_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX2487478.1"
                     /translation="MVLSIYSMNGNELLKVKDEAPRYFDKKSSENLILFESVIGYEDI
                     KKIFKMSLISDKPVHILLVGPPASAKTLFMLEFLKLERSYFTLGSHSTKSGMIDYLFD
                     YRPRYLIVDEIEHMPYKDQTSLLSIMETGILAETKSQKTRSTQLKTWIFATCNNEGKL
                     LSPLLSRFIIFHLKPYTRYKFIEVSNGILVKNDIPFDLANEISHVVWNKLKSRDIRDC
                     IKISRLAKTIEDIEWITKTLIQYRKNK"
     gene            4291..4635
                     /locus_tag="VHJ57_02725"
     CDS             4291..4635
                     /locus_tag="VHJ57_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487479.1"
                     /translation="MTSYNNGKNSHPKGKHAHSTEYRRLIWEMIVWPLIIEKNRPYFS
                     IDEYQSKRVLFSKESNIPSSKLSGGLISLVVKGILIKEEHIYSLHYRLIPYLRKKALL
                     TYGQALKETYTK"
     gene            complement(4673..4755)
                     /locus_tag="VHJ57_02730"
     tRNA            complement(join(4673..4708,4720..4755))
                     /locus_tag="VHJ57_02730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4721..4723),aa:Cys,seq:gca)
     gene            complement(4880..5104)
                     /locus_tag="VHJ57_02735"
     CDS             complement(4880..5104)
                     /locus_tag="VHJ57_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HEX2487480.1"
                     /translation="MGNNPEFGLAVMNRIIKKAGAERVSDEASDTLRKILEEIASNIS
                     KTAIDLANHASRKTIKSDDILLAYKNSYKK"
     gene            complement(5172..>5503)
                     /locus_tag="VHJ57_02740"
     CDS             complement(5172..>5503)
                     /locus_tag="VHJ57_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487481.1"
                     /translation="TIIKNIYQIEIKIILGLSLSDKKELELLLKFHSLNINQIITFSK
                     NLIPSQGNIEDFAYGFIVGIIIGNYLEKFFQRHSRAPDNDELMDIYFTLSLRSAKIRK
                     GILNELT"
BASE COUNT         1934 a          762 c          837 g         1970 t
ORIGIN      
        1 cttcatttgt tagaacttta tcaattgctg aataaggaat ttttatttta ttagtaaaag
       61 gaagaattac cccaaaaaat cctttaagcg ttaaaatgag ataatcatta tgttctaaac
      121 taacattcat cataatacta atctaatatc agttaaaaat aaaaaattta tggactaggg
      181 aaaaagagaa ggtaactgaa agggaggaaa cagcccttgt gtaggtgatt tttcctctaa
      241 ggatggtgat tcctttgatc caaatgtttc attgtctagc gttggtggtt gaagttgtgg
      301 tggttgttgt tgttgttggt tttctagcgt tggtggtggt tgttgcagtt ctaatggttg
      361 gttatcggta ggtggtacca ttgtttgatt ttcggtaggt ggtaccattg tttgattttc
      421 ggtaggtggt accattgttt gattttcggt aggtggtacc attgtttgat tttcggcaga
      481 tagctcatct tctgggggtt gtggtggttg ttgtgtatcc aagtctattc gaggattgtc
      541 caccataagt tgcttggatt tgagatttga ggtagattga ggattgacag aaatagaggt
      601 attagtatta ttaatatcat ctacaattga ttcatttgga ataaacattt ctacggatga
      661 ttccgagagg ttggccaaag gtgaggatgt tgtattttgt tcaacagatg atacactggc
      721 caaaggtgag gaagttgtat tttgttcagc aggtatagtt gtattttttt gattggaacc
      781 aggtagaata gtttgcaatg gaagttgttt agatattgat tctaaggagg ttgtatcttg
      841 ttgtatatca gataataagg aagaattatt tggataacca gtcacattta tagaattcca
      901 ttttgttaac ggcttacttc catccaaaca tgttattttg gatgtcaatt cgttgtttcc
      961 tgcaattatt tcatgataac tcgaatcgta agtgaaaatc cattctgaaa aatcttcaat
     1021 tcctcctgga ccagcagccc taactttttg aaaaggtttt atgtcattcc aatctacaaa
     1081 cacaatacaa tttttatttt gagtatcact agaaatacca gaaatagtta attcaccgct
     1141 aggaattttt ttcccttcat tgggagaggt aatttttaca ctaatatttg caagggtttg
     1201 ggttatttcg ccttgagaat taatcgattg taaatcatag aaaataatta agcccaagaa
     1261 tccaattaaa gaggttagaa aaagagagac gactataaat ttcatagtat agaatcaaaa
     1321 ttacactata tttaaattaa taatatatct cgtagaactt tctactatat tattcgaaaa
     1381 caattgccac gttttttatt tatattaagg attctgagaa agtcagcatc aaagaagaaa
     1441 aaataaaaaa aacattttaa aaaaagtaaa aaacattaca tcgaaatata atgtttatta
     1501 tatgatatca ttattttcct atcttaaaca tttttgttcc acaaacacta catatacctt
     1561 gagtagcagg tcttccgttt ttcattgtaa cctttttttc attttgcatt tcgcgtttag
     1621 atttgcattt aacacaatat ccgtccatat taattcagta aatagaccac aataaaagta
     1681 ttgatgtatt ttttatgaaa tatagaaatt tatggtaaat ggagagtgat aattcacaat
     1741 ttgtaaataa attgatacat acctttcgtt taggaagccc caaatctcct cttcagtagg
     1801 catgtatgtc ttttttatgc tttcttaatt ttttaaatgt agaaaaacga ttgccgcact
     1861 tgtcgcatct ccaatgattt gctcttgctt cttcatatct tgtaaatttt tctgaattta
     1921 cttcaaacat acctgtttta acatcataat ttattggctt ataattaaga cgaaatttta
     1981 atttcgaagg aacagcagac aattttatta tatattaatt gttattaaaa agactgttag
     2041 ctaattttct ctgactttta cttaaaaaaa gaatacatat aattgcgaca ttacaaatgt
     2101 actattgaca tttgttgtta ttattatcct ttttaaaaac gatagtgaac tttccttaaa
     2161 tagataaata tttttatttc ttctatgtag tttttctttg ttgacaattg aaaatatcat
     2221 tcaaaaaagt agtgacatat catcatccat aactccgttt atggcaacta aaataaaaga
     2281 aattctgaaa aaagaacata aagatgatac catagatgtt tattcaaaag agaatgtaaa
     2341 aatatttgat ataatagtta attcaagaag agaactatgt attgcttgtg ttagccttga
     2401 tcagattttt tcctttaatt atattgaagt attgaaaagt atattaaaaa ataatataaa
     2461 aataaaaatt ttgctttgtg atccacattc tgatatgcta aaccatataa aatatttgag
     2521 tgtctttcca aatattcatg aaagccttac ttcctgtctt tccaagctct gtaacatgaa
     2581 gattgtatcg ttatcgacga aagaattatc aaatattgaa ataagaattt ataaaacaat
     2641 attacctcat aatatcataa taagagatag agatattcac ggtagtggac ttatattagt
     2701 agagccctat ttatttggaa taaactcaca aaatagaaaa atctatcaaa tatatagaag
     2761 aaaaaatcaa gaaatttttg atttacattt caaatctttt ttaaagttgt gggagactag
     2821 tcctgaatat tcttgtgaat gggaagaatt gtgaatttta gttgatttgt gtcgcacttt
     2881 tcataagatg acaatgagag aaacatgata actacataat tgctgcttct agtctatctt
     2941 ttagctcata tattgaagat aaattaataa aattgtaccc agttagatta aagtcttctt
     3001 ctttaatgtc accctcctcg atcatttcag ctatttgatt taaagcaaaa agaactaaat
     3061 gtttttcgtc ttcattgatt gaaacatcag ccaatgtata ttcttatgtg taacctatat
     3121 ttttcttttc ctagattaat atctataaat taagaaccta gggaattata gtagtaaata
     3181 aaaataataa atatagattg gtactatcta tttattcaat gaatggaaat gaacttttaa
     3241 aggttaaaga tgaagctcct agatactttg acaaaaaaag tagtgaaaat ttaatacttt
     3301 ttgagagtgt aataggatat gaggatataa aaaagatttt taaaatgtca ttgatatcag
     3361 ataaaccggt acatatttta ctcgtgggtc ctccagcatc agccaagaca ttgtttatgt
     3421 tagaatttct aaagctagaa agatcgtatt ttactttggg tagtcatagt acaaaatcag
     3481 gaatgatcga ttatttattt gattatcgtc caagatattt aatagtagat gaaatagaac
     3541 atatgccata caaggaccag acatcattac tgagtataat ggagacaggg atactagctg
     3601 aaacaaagtc acaaaaaact cgtagtaccc aattaaaaac atggatcttt gcaacatgca
     3661 ataacgaagg aaagttatta tcaccacttc tttctagatt tattattttc catctaaaac
     3721 catatactag atataaattt atagaagtat cgaatggaat tcttgttaaa aacgatattc
     3781 cttttgatct tgctaatgaa atttctcacg ttgtttggaa caaattaaaa tctcgagata
     3841 ttagagattg tataaaaatt tctagattag caaaaacaat tgaagacatt gaatggatta
     3901 caaaaacttt gattcaatat cgaaaaaata agtaagaggc agtatcatac gaataaagtt
     3961 attcttgtga tttgttagag gtatttttag tccgtttcct tcttaaccaa aatgcagtaa
     4021 ttattaaacc gaccagtatc gaaagataag aaaaaaaaat tgtttcattt atgacagtgt
     4081 ccgggggtag tattgcgaat aagaattgat gtagactcat atttaatccg gaatattcaa
     4141 ctgttatatt gttttctaaa tcctataatt tgtatataga tttttacaac tttctctacg
     4201 tagtattcta ttatcgataa agatattcaa tttaaaagta ttagattata aaacctattc
     4261 caacattaaa gacataaaaa tacatgaaca atgacatcct acaataatgg caaaaattct
     4321 catcccaaag gaaaacatgc tcattctact gaatatagaa gattaatttg ggaaatgatt
     4381 gtttggccat tgataattga aaaaaataga ccatactttt caatagacga ataccaatcc
     4441 aaaagagttt tattttcaaa agaaagtaat ataccttctt cgaaattatc tggtggatta
     4501 atatcattgg tagtaaaagg aattttaatt aaggaagagc atatatattc attacactat
     4561 aggttgattc cctatctcag aaaaaaagct ctcttaactt atggccaagc attgaaggaa
     4621 acttatacca agtaaatccc ttattctaga catctattta aaaataattc aaaggtgggg
     4681 aagggattcg aacccctata tattcgctat caacaatttc tgcaggcgaa cgcataaccg
     4741 ctctgccacc ccaccttata attagtcttg gaaaagagac atataaatga gttatttacc
     4801 tttagcaaac tatttcttaa taaattagag aaatatttga ctgttaatat ccgctaaaac
     4861 aatctagatg aaatatactt cattttttat aactattctt gtaggccaat aaaatatcat
     4921 cagattttat tgtttttctg ctcgcatgat ttgcaagatc tatagcggtc ttagatatat
     4981 tgcttgcaat ttcctcaagt atttttctta aagtatctga tgcttcatca cttactcttt
     5041 ccgcacctgc ctttttaatt atcctattca ttacagccaa tccaaattca gggttattac
     5101 ccatagtata aacagtctaa ggatatttaa aaatctaatt gatattatac gtcagaatca
     5161 ctaaaaaata attaagttaa ttcatttagt atacccttcc taatttttgc agaacgcaat
     5221 gataatgtaa aatatatatc cattaattca tcattatcag gagctctact gtgtctctga
     5281 aaaaattttt ctaaataatt accgattatt atgcccacaa taaagccata agcaaaatct
     5341 tcgatattac cttgcgatgg aataagattc ttagagaaag ttattatttg atttatgttt
     5401 aaagaatgaa attttaataa taattctagt tcctttttat cggataagct cagtcccaaa
     5461 attattttaa tttctatttg atatatattt ttaattattg tgt
//
LOCUS       DATAFW010000114         6268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4997079, whole genome shotgun sequence.
ACCESSION   DATAFW010000114 DATAFW010000000
VERSION     DATAFW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4997079"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VHJ57_02745"
     CDS             complement(<1..267)
                     /locus_tag="VHJ57_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX2487482.1"
                     /translation="MEIKILGAAKEVGRSAFLVSNENSNILMDYGVLLKKEPQFPLHV
                     KPKELHAVVLSHAHLDHSGFLPSLFLSSSKFPVLSTNPTIELSQL"
     gene            complement(330..1166)
                     /locus_tag="VHJ57_02750"
     CDS             complement(330..1166)
                     /locus_tag="VHJ57_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487483.1"
                     /translation="MDYNFTNLINAFTQLKKFRSYSLSRHHERFLFPYICGTYFSYRK
                     VDVLRIKSIADAISNNLTDSIKYLEVGCGNGDFLEKVREYISNAKGIESNIDIFYSFG
                     KIKPEYIEIMDVKYGIDEFQDIIFVGWMDPGQDFRKNIAEKTDIIITTLDPGLSLAAE
                     YEGHGFKKIVKWTTPSWEDVNIEINNQFYSNLDSKSLQFLSDLRGAHNFWYVYSKDNV
                     KSHLIIDNLKKQILHEENKEGKIIPYDFEKILDECGFSFHQCLYNNYVNSKTILWKID
                     FL"
     gene            complement(1256..1516)
                     /locus_tag="VHJ57_02755"
     CDS             complement(1256..1516)
                     /locus_tag="VHJ57_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2024 family protein"
                     /protein_id="HEX2487484.1"
                     /translation="MECAVYDTYVTKKDGKIMHFDVIVEASAPHEKAIEYGKEYLEQV
                     GQGNQTMTQEECQFCHVQEAPPVVAKDIENRGYFIQKMEGCP"
     gene            1670..1960
                     /locus_tag="VHJ57_02760"
     CDS             1670..1960
                     /locus_tag="VHJ57_02760"
                     /inference="COORDINATES: protein motif:HMM:NF015632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HEX2487485.1"
                     /translation="MKPKKEIRDKENTERLESTIATLESFKENKDLPRVIRNQIKDIY
                     DILKEEKNGSISVRAANAVSMLDSLTQNRHMESHIRTSLWQVVSKLESIREQ"
     gene            2264..2884
                     /locus_tag="VHJ57_02765"
     CDS             2264..2884
                     /locus_tag="VHJ57_02765"
                     /inference="COORDINATES: protein motif:HMM:NF034126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex RNA-binding protein Csl4"
                     /protein_id="HEX2487486.1"
                     /translation="MIKKKKNYDVIFPGEHLAFIEEFESGKNTFVVDGSIRSLSFGKK
                     EYDFKRRQVVIERIKQAHFPKIGDVIVGYIEMLFGSMISIRILYLNNRKVSSGLSAIA
                     STRIASESGGWNSRRDRGERRHKLVYRVGDIVRGRIFSLMNSSSHATISDKDLGVVYC
                     ICFICGGDTVKSNNTVKCIECGMIEEKKLAVDFGKEVLLSLAKKRG"
     gene            3052..5337
                     /locus_tag="VHJ57_02770"
     CDS             3052..5337
                     /locus_tag="VHJ57_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX2487487.1"
                     /translation="MTRNYKIYKNNMVRFILISFLISFTIISLNLFQINSVEAQIGKF
                     GTFNGNNFKYVKDSELFQLNQYSIAVWFKTNNTDPHVSFLVNKGGFGSEAAGENMNYG
                     LWITYLGHLQAGFEAKDSSNYFVTSKKPVNDNKWHYATVTYDGSLLKLFIDGVLDHSI
                     NTNNVNPDITGKNRIAIAANSLFKDKLYIGDVDEVRIWDRSLTTAEVYDAYTQNKFNL
                     DKQLVFVSFGDNTSCKPEKAVNVTAIGHAANNTQLNAIDGNQSTRWSNYGLGSWVQVD
                     LGKEKEICDIDISWYKGDHRKINFTISTSVDGEIFSNLFSETSSGLTNGPEKFDIPDV
                     KTRFLKLTVNDNSMNEWSSISEIDINNPIVPGSPSPCSLLNIPVDHISSIDSSPNNTE
                     GNSVDNDLSTKWSNLGLGSWLQFDLGQEKQICDLGISWYKGNIRSIDFLVSVSSDGSD
                     FRQVFSSTNSNTTDDIEFYDLPDTVGKYLRISVTGNSVNDWASINEVDIFANPIQKES
                     FTSASLINIVTQVINDNGGTNQTKDFIISINGSQATPNSFPGNPDGQSVSLQPGTYQV
                     KINNFSGYKATFSAGCKGIIGSDDNIECVITLDDMDPATPYNESLNTIDNLTSNNLTS
                     NNLTTTITTTNKNINDSNNNNNANVNLLLRDLDLNVLLEVINVHGGNKKPLDFIITVK
                     GENVVPSSFAGSNNFQKVSIKAGNYDVKISDTLGYEINRISGCTGVIERTDKTCIIEL
                     IDTPKLLDTTQRNNISENITH"
     gene            complement(5639..5923)
                     /locus_tag="VHJ57_02775"
     CDS             complement(5639..5923)
                     /locus_tag="VHJ57_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487488.1"
                     /translation="MVLEVGGEELDEEELGGSNEFRGEEELDEEELGGSNEFRGEELD
                     EEEFTEGDEPIPCINESIVLSIILPLPTLFKRLLISVSMLVGLEPFSDCE"
     gene            5952..>6268
                     /locus_tag="VHJ57_02780"
     CDS             5952..>6268
                     /locus_tag="VHJ57_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487489.1"
                     /translation="MSSSSLNSLLPPSSSSSSSSSPLNSLLPPSSSSSSSSPPNTSSL
                     PNTSNVVNSELLSILKNQDSSQSSVTVNQVKNQNMKYFAKIVDKKSNNEDIISSNSLT
                     EFN"
BASE COUNT         2240 a          935 c          922 g         2171 t
ORIGIN      
        1 taattgagat aattcaatag taggattagt tgaaagaaca gggaatttag atgatgataa
       61 aaataaagag ggaagaaagc cagaatgatc taaatgagcg tgagataata ctaccgcgtg
      121 taattctttt ggtttaacat gaagtggaaa ttgaggctct tttttcagaa gaactccata
      181 atccattaaa atgttagaat tttcattact gactaagaag gccgaacgac caacctcctt
      241 agcagctcct aaaattttaa tttccattat tattctatga tactaataag tatggttact
      301 ttaagtcttg ttatattttt tgttgttgtt tagagaaaat ctattttcca taatatagtt
      361 ttagaattta catagttatt atataaacac tgatgaaagg aaaatccaca ttcatcaaga
      421 attttttcga aatcatatgg tataatcttg ccttctttat tttcttcgtg taatatttgt
      481 ttttttaggt tatctattat taaatgactt tttacattat ctttagagta gacataccaa
      541 aaattatgtg ctcctctcaa atcagaaagg aactgtaaac ttttggaatc aagatttgaa
      601 tagaattggt tattaatttc aatatttaca tcttcccaag atggtgtagt ccatttgact
      661 atttttttga aaccatgtcc ttcatattct gcagctaagc ttaaaccagg gtccagggta
      721 gttattatta tatctgtttt ttcagctatg ttttttctaa aatcctgacc aggatccatc
      781 caacccacaa atatgatatc ttgaaactca tctataccat atttcacatc cattatctct
      841 atatattctg gtttaatttt accaaatgaa taaaatatat ctatattact ttctattcct
      901 tttgcatttg aaatatattc tctcaccttt tctaaaaaat ctccatttcc acaacctact
      961 tctaaatatt ttatagaatc agttaagtta ttactgattg catctgctat tgattttatt
     1021 ctcaagacat ctactttgcg ataagaaaaa tatgttccac atatatatgg aaaaagaaat
     1081 cgctcatgat gtcttgatag tgaataagat ctaaattttt ttagttgagt aaatgcattt
     1141 attaaatttg taaaattata atccattata aatttattaa ggtattgcta aaccatcact
     1201 acaataagtc cgagttaaaa tttaaatgca gtgaaaaaga ctataaataa aaaaattatg
     1261 gacaaccttc cattttttga ataaagtatc ctctattttc tatatccttc gcaactacag
     1321 gtggagcttc ctgaacatga cagaattgac attcttcttg ggtcattgtt tgatttccct
     1381 gacctacttg ttctagatat tcctttccat attcaattgc tttttcatga ggagcactag
     1441 cttctacaat tacatcaaaa tgcatgattt ttccatcttt ttttgttaca tatgtatcat
     1501 aaactgcaca ttccatagta taaataaata tttaggaata tttattctat actagatctt
     1561 ttaaaatcat tattctatta aatttaaatt tatgagaatt aaaaacatat aaataactaa
     1621 tcatcaacta aattttattc tttacaaaat cgaggtttag tttgctgtta tgaaaccaaa
     1681 aaaggaaatc cgtgataaag agaacacaga aaggcttgag agtacaattg caactttaga
     1741 atcatttaag gagaataaag acctccctag ggtaatacgc aaccaaatca aagatattta
     1801 tgatatttta aaagaagaaa aaaatgggag tatatctgtt agagcagcta atgcagttag
     1861 catgctagat agtctaacgc aaaatagaca tatggaatca catatcagaa cgtctctttg
     1921 gcaagtagtt tcaaagttag aaagtataag agaacagtaa tagaaaatac tattttatta
     1981 ttcataaata tcatgaattt gcttaagttt ttcaaatttg ataatttaaa aagggaatta
     2041 agaaaaggta acttcaaatt aatttttgct tcaggaatca gtttaattct ggttggaatt
     2101 ttttttatag tattaatatc tcacttactt ttcaattaag gatagtagaa cgttatatac
     2161 atacaatact gaaattttat taaatcatcc cacttttact gtttataaaa gtagaataat
     2221 gtaaaaataa caattataac tttaagaaat atacaattct taaatgatta agaaaaaaaa
     2281 gaattatgat gttatatttc ctggtgaaca tcttgcattt attgaagaat ttgaaagtgg
     2341 aaaaaatact tttgttgtag atggttctat aagatcttta agctttggga aaaaagaata
     2401 tgattttaaa agaagacaag ttgttataga aagaataaag caagcacatt ttccaaaaat
     2461 aggggatgta atagtcggat atatagaaat gctatttgga agtatgattt ctattcgaat
     2521 attatatctc aataatagaa aagtttcgtc gggtctatct gcaattgcct caacaaggat
     2581 agcctcagaa tcaggaggat ggaattccag gagagataga ggtgagagaa gacataaatt
     2641 agtttataga gttggggata tagtaagagg tagaatcttt agcttaatga actcttcctc
     2701 ccatgcaact ataagtgata aagatttagg tgttgtctat tgtatatgct ttatctgtgg
     2761 aggtgatacc gtcaaatcta ataatactgt taaatgcatt gagtgtggaa tgattgaaga
     2821 aaaaaaattg gctgttgatt ttggtaaaga agtcttatta tcattggcaa aaaaaagagg
     2881 ataatatata taatttttga gcaagttatt cttattatta ttttttaaat aattagtaaa
     2941 tttattttat atttttatca actatttttg gtaaaataga ttataggata tcttggtaaa
     3001 tttctgaaag tgataaaaga aatttatttg actaattctt ggtgagagat aatgacaaga
     3061 aattataaaa tttataaaaa taacatggtt agattcattt taatttcatt tctaatttca
     3121 tttaccatta tttctttaaa tctttttcaa attaatagtg ttgaagcaca aattggaaaa
     3181 tttggaacct ttaatggtaa taattttaaa tatgtaaaag attcagaatt attccaatta
     3241 aatcaatatt caattgctgt gtggttcaaa acaaataata cagatcccca tgtttcattt
     3301 ttagttaata aaggtggatt tggatccgaa gctgccggtg aaaatatgaa ttatggactt
     3361 tggataacct atctaggtca tcttcaagca ggatttgaag caaaagactc ctcaaattat
     3421 tttgttactt ctaaaaaacc tgttaatgac aataaatggc actatgctac agtcacctac
     3481 gatggatcat tactaaagtt gtttatcgat ggagtgctcg accatagtat aaatacaaat
     3541 aatgtaaacc ctgatattac aggaaaaaat aggattgcaa tcgctgcaaa ctctctcttc
     3601 aaagacaagt tatatattgg tgatgtagat gaagttcgca tttgggatag atctcttact
     3661 acagcagagg tatatgatgc atatacacaa aacaaattca atttggataa gcaattagta
     3721 tttgtttcat ttggcgataa tacatcttgt aaacctgaaa aagctgttaa tgttaccgcg
     3781 ataggacatg ctgctaataa tacacaactc aatgcaattg atggtaatca atctacacga
     3841 tggtctaact atggacttgg ttcttgggtt caagttgatt taggtaagga aaaggaaata
     3901 tgtgatattg atatatcttg gtataagggt gatcatagga agattaattt cactatctct
     3961 acgtctgtag atggagaaat ttttagcaat ttattttctg aaactagtag cggacttacc
     4021 aacggacctg aaaaatttga tattcctgat gttaaaaccc gatttcttaa attaactgtt
     4081 aatgacaatt caatgaatga gtggtcaagt atatctgaga ttgatattaa taatccaata
     4141 gttcctggct ctccttctcc atgttcactt ttaaatatcc ctgtcgatca tatttcatca
     4201 atagatagtt cacctaataa tactgagggc aattcagtag acaatgacct ttccacaaaa
     4261 tggtcaaatc taggattagg ttcatggtta caatttgatc ttggacaaga aaagcaaata
     4321 tgtgatcttg gaatttcttg gtataaagga aatatccgtt ccattgattt cttagtctca
     4381 gtgtcttcag atggttctga ttttagacag gttttttcta gcaccaatag taatactact
     4441 gacgatattg aattttatga tctcccagac accgttggta agtatcttag aattagtgtt
     4501 actggaaata gtgtaaatga ttgggcaagt atcaatgaag ttgatatatt tgctaaccca
     4561 attcaaaaag aatcctttac ttctgcatca ctcataaata tagtgactca ggttatcaat
     4621 gataatgggg gaactaacca aacaaaagat tttattatat caataaatgg cagtcaggca
     4681 actccaaatt cattcccagg taatcctgat ggacaatctg tatctctaca accaggaact
     4741 tatcaagtca aaataaataa cttctctgga tataaagcta ctttttcagc tgggtgtaaa
     4801 ggtattatag gttcagatga caacatagaa tgtgtgataa cattggatga tatggatcca
     4861 gcaacaccct ataatgaatc actaaacact attgataatc ttacaagtaa caatcttaca
     4921 agtaacaatc ttacaactac aatcactaca acaaataaga atattaacga cagtaacaat
     4981 aataacaatg caaatgtaaa cttactttta cgtgaccttg acttgaatgt tctattagaa
     5041 gtaataaatg tccacggtgg aaacaaaaaa ccattagatt ttattataac agtcaaggga
     5101 gaaaatgtgg taccaagttc ctttgcaggt agcaacaact ttcaaaaagt atctataaag
     5161 gcaggaaatt atgatgttaa gatatctgat acattaggat atgaaataaa tagaatttct
     5221 ggatgtaccg gagtaataga aagaacagac aaaacgtgta taatagaatt aattgatact
     5281 ccaaaactat tagacaccac ccaaagaaat aatatctcag aaaatataac acattaatcc
     5341 ggctagtata ttgacaatat tttttaatat gttaaatttc ttaaattttt tgtatgtttc
     5401 taaaaatatt gtaattgttt tattttgtat tgtgcattta ccaattatta aattttatag
     5461 aaaaagatcc tataatatta taggagattt aataataact tttttacaat tcatattaat
     5521 actaaaagat ataagatcgc tcgaggtaga atatttaaat gaaatttaaa tttttggtat
     5581 tgtatggttt tatcttatta gtatctataa gtaccggtga tcaattatta aacttcggtt
     5641 attcacaatc agagaatggt tctaatccga ccaacattga tacagaaatt aataatcttt
     5701 taaataaagt cggaagaggc aatataatag atagtacaat tgattcatta atgcaaggaa
     5761 tgggttcatc cccttcagta aactcttctt catccagctc ttctcctcta aactcattac
     5821 ttccacccag ctcctcttca tccagctctt cttctcctct aaattcatta cttccaccca
     5881 gctcctcttc atccagctct tctcctccaa cttctagcac cacaacaaat tcttctgggg
     5941 caaactcttc tgtgtcaagc tcatctctaa attcattact tccacccagc tcctcttcat
     6001 ccagctcttc ttctcctcta aattcattac ttccacccag ctcctcttca tccagctctt
     6061 ctcctcctaa tacatcctca ctgcctaata cctcaaatgt tgtgaatagt gaactattat
     6121 caattttaaa gaatcaagat tctagtcaat cttccgttac cgttaatcaa gtaaaaaatc
     6181 aaaatatgaa atattttgca aagatagtag ataaaaaaag taataatgaa gatattatct
     6241 ctagtaattc attaacagaa tttaataa
//
LOCUS       DATAFW010000115         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1649181, whole genome shotgun sequence.
ACCESSION   DATAFW010000115 DATAFW010000000
VERSION     DATAFW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649181"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VHJ57_02785"
     CDS             complement(<1..219)
                     /locus_tag="VHJ57_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX2487490.1"
                     /translation="MTRLFDELGLNKKLLLAIKESGYTTPFPIQEKAIPIILEGNDVI
                     GQAHTGTGKTAAYSLPILMKMKNARNVQA"
     gene            410..1321
                     /locus_tag="VHJ57_02790"
     CDS             410..1321
                     /locus_tag="VHJ57_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487491.1"
                     /translation="MMAEDEELKEKKLRGSGGYVIARISDNEQKKGNLGGPELFIAGI
                     GRLPKDRILKYYCNKCEKDYPTSPDLNYENPNEDVGEGVVLIEKGEYKCSVCKAVISQ
                     YRKFNNETAPPSPSKTNSLSQSKKSSEVAKSVNNVSDSSPKNKSKSEKSILPSKTFEE
                     NTSDSIQSESIDTKSSNITIAKGKYFPIQSIIGMPVYDHEAMLVGNVQEIGLRKSLNG
                     TIQITLKIDNRDRTSETNGDESYNDEITWSNISKIGDIVLISREQNKIPSSSNTSSPI
                     DKKICISCQYHNESDALYCEQCGKKLE"
     gene            complement(1346..>3143)
                     /locus_tag="VHJ57_02795"
     CDS             complement(1346..>3143)
                     /locus_tag="VHJ57_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX2487492.1"
                     /translation="LEKQIGDIGKQYGIRIIGPNCLGIMSLSQSSMMNATFLKITPEV
                     GGIALVSQSGAICAATVEDAIAQGIGFSKVISMGNKVDMDENDILELLSEDDDTKVIV
                     MYLEDIHDGKRFMQIAKRITKEKKKPILVLKAGRTPEGAKAAMSHTGALMGSDETYDA
                     LFKQSGVIRVDTMQELFETSTAFEKQPIPTSNGGVVILSNAGGPAIISTDACSKYGLK
                     MADISQSESEIAKVIPPHGSARNPVDIVGDADFTRFEKVLNEVLSNDNVSAVVTMCTP
                     SATLDYNELSKTIIKTSKDSGKTMLAALMGLAEGIENKKILSDGGIPHFMYAEPAIRT
                     LEAMFRHSRWIEKEDTPPKKFEVDKTKARNVLENVIKSNRNNLLEEEGYEILKAYGFS
                     TPKKFLATDEEESARAAEEIGYPVVMKIVSPQIIHKSDAGGVKIGIQNQQEARKAFNE
                     ILLNVKKYKSDAEIKGVLVQEMIGSAKETILGAKRDPLFGPLIMFGLGGIYVEVLKDV
                     IFRIAPIDEQQAKEMVESIKTIKILKGARGEKESDIDGIVESIQRLSQLVTDFPEIEE
                     FDMNPLLVLEKGNGVRAVDVRIGLKNQKDKTI"
BASE COUNT          983 a          561 c          424 g         1175 t
ORIGIN      
        1 agcttgtaca tttcttgcat ttttcatttt cattaaaata ggtaatgaat atgctgcagt
       61 ttttcccgtt ccagtatgtg cttgtccaat tacatcgttt ccctccaaaa taataggaat
      121 tgctttttct tgaataggaa agggtgttgt atatccactt tcttttatag ctagtaacag
      181 ttttttattt agtcccagtt catcgaatag tcttgtcatt aaatttcact agtttaataa
      241 accctctttc atcttatcta atctaaatct ttgtgaattt gaaaaaattc atgatgatat
      301 tatgacgaca ttattaagta attaaaattc ttttaaacat aatattaaaa taagaagata
      361 atacaaacac taatacgact gtttttgcgt tattatgatg atatatttaa tgatggctga
      421 agacgaagaa ttaaaagaaa aaaagctacg tggctctgga ggctatgtta ttgccagaat
      481 ttcagataat gaacaaaaaa aagggaatct gggtggacct gaattattta tagcaggtat
      541 tggacgatta ccaaaagaca gaattttaaa atattattgc aataaatgtg agaaagacta
      601 tccaacttcc ccagatttaa attatgaaaa tccaaatgaa gatgtcggtg aaggagttgt
      661 tttaattgaa aaaggtgaat acaaatgctc agtttgtaaa gcagttattt ctcaatatcg
      721 taaatttaat aatgaaaccg ctcctccttc tcctagtaaa actaattctc tatctcaatc
      781 aaaaaagtca tcggaagtcg ctaaatctgt aaataatgtt tcagattcat cacctaaaaa
      841 taaaagcaaa tctgaaaaat ctatactacc ctctaagact tttgaagaaa atacttctga
      901 ttcaatacaa tctgagagta tagatacaaa atctagtaat ataactatcg ctaaaggaaa
      961 atattttcct attcagtcaa ttattggaat gccagtttat gatcatgaag ctatgctagt
     1021 aggcaatgtc caagaaatag gacttagaaa atcgcttaat ggtacaatac aaattacatt
     1081 aaaaattgat aatcgcgata gaacatctga aactaatggt gatgaatcat ataatgatga
     1141 aattacttgg tctaatattt ctaaaattgg agatattgtt ttaataagta gggaacaaaa
     1201 taaaataccc tcctcatcta atacttcttc tcctatagat aaaaaaatat gtatttcatg
     1261 ccaataccat aatgaatcag atgctcttta ttgtgaacaa tgtggtaaaa aactagaata
     1321 atatttttat tccaattttt ttttattata tcgttttatc tttctgattt tttaatccta
     1381 ttcgaacatc tactgctctt acaccattac ctttttctaa tacaagtaac ggattcatat
     1441 cgaattcttc aatttctgga aaatcagtaa ctaattgtga tagtcgttga atactttcta
     1501 ctattccatc tatatctgat tccttttctc ctcttgctcc ttttaatatc ttaatagttt
     1561 taatagattc taccatttct tttgcttgtt gttcatctat tggagctatt ctaaatatga
     1621 catcttttag aacttcgaca tatattccac ccaaaccaaa cattattaat ggtccaaata
     1681 atggatctct tttcgcacct aaaattgtct cttttgcaga acctatcatt tcttgaacta
     1741 aaactccttt gatctctgcg tctgatttat atttttttac atttaataat atttcattaa
     1801 atgcttttct agcttcttgt tgattttgaa ttccaatctt aacacctcca gcatcggatt
     1861 tatgaatgat ctgcggagaa acaattttca taactactgg ataacctatt tcttcggctg
     1921 ctcttgccga ctcttcttca tctgtagcca gaaatttttt tggagttgaa aatccatacg
     1981 cttttaatat ttcatagcct tcttcttcaa gcaaattgtt tctattagat tttatcacat
     2041 tctctaaaac atttcttgct tttgtcttat ccacctcaaa tttttttgga ggagtatctt
     2101 ctttttcaat ccatctagag tgtctaaaca ttgcttctaa tgttcttatt gctggttctg
     2161 catacataaa atgaggaatt cctccatctg aaaggatttt tttattttct attccttctg
     2221 ctaatcccat taaggctgcc aacattgttt ttccagaatc tttggatgtt tttattattg
     2281 tttttgataa ttcattataa tctaatgttg cagaaggtgt acacatagta actactgcgc
     2341 taacattatc attagataag acctcattta gcactttttc aaatcgagta aaatctgcat
     2401 ctcctactat atcaacaggg tttctagctg aaccatgagg aggtatgact ttggctattt
     2461 ccgattctga ttgagaaata tccgccattt ttaatccata ttttgaacaa gcatctgtag
     2521 aaattatggc tggcccaccc gcatttgata atatgactac tccaccattt gaagtcggaa
     2581 ttggttgttt ctcaaaagct gttgatgttt caaataattc ttgcatggtg tctactctta
     2641 taactcctga ttgcttaaac aaagcgtcgt atgtttcatc agaccccatc aaagcaccgg
     2701 tgtgtgacat agcagctttt gctccttcag gtgtccttcc tgcttttaat acaagaatag
     2761 gtttcttttt ttctttagtt attcttttag ctatttgcat aaatcttttt ccatcatgaa
     2821 tatcctctag atacatgaca attacttttg tatcatcatc ctctgaaagt agttctaata
     2881 tatcattttc atccatatct actttatttc ccatgctaat tacctttgaa aaacctattc
     2941 cttgagctat agcatcttca acggtagcag cgcatatagc accactttgt gataccagag
     3001 ctattcctcc aacttctggt gtaattttta aaaatgtagc attcatcata ctgctttgag
     3061 ataagctcat tattcctaaa caatttggac caattattct tattccgtat tgtttaccaa
     3121 tatctccaat ttgcttctca agt
//
LOCUS       DATAFW010000116         5601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2003469, whole genome shotgun sequence.
ACCESSION   DATAFW010000116 DATAFW010000000
VERSION     DATAFW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003469"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..192
                     /locus_tag="VHJ57_02800"
     tRNA            join(66..84,101..135,172..192)
                     /locus_tag="VHJ57_02800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Met,seq:cat)
     gene            complement(213..1580)
                     /locus_tag="VHJ57_02805"
     CDS             complement(213..1580)
                     /locus_tag="VHJ57_02805"
                     /inference="COORDINATES: protein motif:HMM:NF020078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HEX2487493.1"
                     /translation="MNTNTNDNNILHIGFDDTDSTLGKCTTHLAFNVISYLNKKNVKF
                     LDYPLLIRLNPNIPWKTRGNGAVCLRIMTKAKNNENIIDFIKNELEKNSEIGKGANPG
                     LVCLQSKDIPDEIKEFHRLAMFDILSKQKATKLAQKYNLNYFSFGNGQGLVGAIAAIG
                     CLLNSDHTFEILAYRKIKNCGTLRIIDPINVIRLHDTTFPSTFNSYDYKHKRVLILPH
                     GPDPVFCGIRGEDPQVLHNSLKILDIKEDLAGYMIYRSNQGTGMHLQNELKLSMIKSF
                     TSGYSNCVIVTNPQIIQGGHVLFTVEDKLKNICLAVVYEPTGLTTIASQLHIGDQIEI
                     GFGVRKATSKHSKVLNIEYIFIQQLVQIYNTVNPVCMNCKKRMKSEGTNKGYQCKKCK
                     IIDKNGKKIHLLKTRSIKEGLYIPTLKAHRHLTKPFHRYGKEKTRFFEFGNVELYSKW
                     FKRFR"
     gene            1713..2054
                     /gene="hisI"
                     /locus_tag="VHJ57_02810"
     CDS             1713..2054
                     /gene="hisI"
                     /locus_tag="VHJ57_02810"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX2487494.1"
                     /translation="MQFKVLEDLDFTKRGGLLPVIVQDQITKDILMLAYVNREALENT
                     IKTGNAWFWSTSHKKLWMKGEESGNIQPIEKILVDCDADALVYIVKSNKPICHTGSNS
                     CFHNFLKDKNY"
     gene            2222..3445
                     /gene="thrC"
                     /locus_tag="VHJ57_02815"
     CDS             2222..3445
                     /gene="thrC"
                     /locus_tag="VHJ57_02815"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX2487495.1"
                     /translation="MGIIQSLICRECKKEYKPQFRYICEDCFGPLDVKYIYPNNINRE
                     LFGSRKEKTYWRYFELLPIRDKNNIISINSGFTPLQKSTNLSKDLQLKSLYIKNDSVN
                     PTFSFKDRPAGVAVSRAKEINLKAVGCASTGNLASATAAHAAVARLPCYIFAPADLES
                     VKISQALAYGAEFVAVEGTYDDANRVASVIGDSKGIGIVNINMRPYYVEGSKTLAYEV
                     AEQLNWQVPDYLVIPVGSGAMLNAICKGFEELCEIGLINDVSNLKIIAAQPHGCSPIV
                     DAFKSNSNEIFPVEKPNTIAKSLAIGDPGDGIYVLKRLKQYHGIAESATNQEIVNGIL
                     KLSKTEGIFTEPAGGVSVAVLQKLIESGKIDRDANIVCYVTGNGLKSIEAIVGMLPKP
                     STVKPDVQMVSTIIR"
     gene            3495..4889
                     /locus_tag="VHJ57_02820"
     CDS             3495..4889
                     /locus_tag="VHJ57_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX2487496.1"
                     /translation="MAKIEFIIPSVLNKGSGEKKILLDVQDLHDAFNKASDNLGDDFK
                     RRVLDLNGKPRALINIYINGKNMRFNKDGMTTSLKDGDSIYILPAVAGGANETKIEHI
                     ELSSQDLQRYSRQIMLEEIGFIGMEKLRNSKVCVVGVGGIGNPVVTQLTAMGVGKIRI
                     VDRDVVEISNLHRQHLYTEQDIGKVKVEAAKEHLKRRNTDVEIEALPISITNYTAENI
                     IKGFDVVIDALDSIDARYALNDACIKQHIPLIYAGALGMLGSVCTILPNQSACLRCIF
                     PALSEDDMPTCSTEGVHPSILYLVGGIQVSEAIKILLGQKPSLINTLLYIDLNELSFE
                     RIKMLRHDECSACGKEKIIKEIQVEKEQDLIIEELCGRDRGKRTYTVTPSKITSIVNL
                     NKVINNAELLGYNIKSRGSLGITVTTNNSNPSQQLSISFMTSGTATIVGAKDENAALA
                     IYNEFLNFKISNKK"
     gene            complement(4917..5576)
                     /locus_tag="VHJ57_02825"
     CDS             complement(4917..5576)
                     /locus_tag="VHJ57_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX2487497.1"
                     /translation="MHVQDNRYSEYIANQIKKGTTTCALSCSDGVVLAADTRASAGMF
                     IADKHVMKIQNVDKHLGMTIAGGVADAQNLVDTMRYNANLYRINNKRIIPVKSAARLC
                     SNILFNQRYFPYYVQIIMAGYEGKGTGQVYNIDLFGSLTEEKFISTGSGSPVAYGYLE
                     SEFKENMSVDEAYMVALQAIAAAIRRNAGTGDGINVVIIDKNGYRELSKEVKEAVKVV
                     F"
BASE COUNT         1959 a          825 c          874 g         1943 t
ORIGIN      
        1 ttatcgaaaa atattttaaa tttcaataga cagagatata tagttgtaaa atcaaaaaag
       61 gaatcagcgg ggtggggaag ccagatatat aataggcaaa gttatcccgg cgggctcata
      121 acccgcagag cggtttctac caaagtaaat attgctacac ggtagccgta agttcaaatc
      181 aaccccccgc tatttgttgt tttttagttt tattatctaa atcttttaaa ccattttgaa
      241 tacaattcta catttccaaa ttcaaaaaat ctagtctttt cctttccata tctgtgaaaa
      301 ggtttggtta aatgtctatg tgcttttaat gtaggaatgt ataatccttc tttaattgat
      361 cttgttttca ataagtgtat ttttttaccg tttttatcga ttattttgca ttttttgcat
      421 tggtaccctt tatttgtccc ttctgatttc attctctttt tacaattcat acaaactgga
      481 ttaacagtat tataaatttg tacgagttgt tggataaaaa tatattcaat atttaatacc
      541 tttgaatgtt ttgatgtagc ttttctaaca ccaaatccga tctctatttg atctcctata
      601 tgtaattgtg aagcaattgt agttaatcct gttggttcat atactactgc caaacaaata
      661 ttttttaatt tgtcttcaac ggtaaataat acatgtccac cttgaataat ttgtggattt
      721 gttactatta cacagttaga atatccagaa gtaaaagatt ttatcatgga caatttcaat
      781 tcattctgta aatgcattcc agttccttga ttagaacgat aaatcatata tcctgctaga
      841 tcttctttga tatccagaat tttcagacta ttatgtaata cctgaggatc ttctcctcta
      901 attccacaaa acaccggatc aggtccatgt ggtaatataa gaactcgttt atgtttataa
      961 tcataactgt taaatgtaga gggaaatgtt gtatcgtgga gacgaataac atttattgga
     1021 tcgattattc taagagttcc acaattttta atttttctat atgctaatat ctcaaatgta
     1081 tgatctgaat tcaataaaca tccaatagca gctatagctc caactaatcc ttgaccattt
     1141 ccaaaactaa aatagtttaa attatatttt tgtgctagtt ttgttgcttt ttgtttacta
     1201 agtatatcaa acattgcaag tctatggaat tccttaattt catcaggaat atccttgctt
     1261 tgcaaacaaa ccaagccggg attagcacct ttaccaattt cagaattttt ttctagctca
     1321 tttttaataa aatcaattat attttcatta tttttagctt tagtcattat cctcaaacaa
     1381 acagcaccat ttccacgagt tttccaggga atattggggt ttaatcttat taacaaagga
     1441 taatctaaaa atttcacgtt ttttttgtta agataagata taacattgaa agctaaatga
     1501 gtagtacatt ttcccaatgt tgagtctgta tcatcgaacc ctatatgcag aatattattg
     1561 tcatttgtat ttgtgttcat caacatttct aaataatatt aaatcattaa ataaatttaa
     1621 tttctttttg aaataatgat tagaaattaa aattatattc actattttgc ataattactt
     1681 ttttatattg atcatgaaat aaaagatata ttatgcaatt taaagtcttg gaagatcttg
     1741 attttacaaa acgtggggga ttattaccag taatagttca agatcaaatt actaaagata
     1801 ttcttatgct tgcatatgtt aatagagagg ctcttgaaaa tactatcaaa actggcaatg
     1861 catggttttg gagtacctct cataaaaaat tatggatgaa aggtgaagaa tcaggaaata
     1921 ttcaacctat agagaaaatc ctagttgatt gtgatgctga tgcattggtg tatattgtaa
     1981 aatccaataa acctatatgt catactggaa gtaattcttg ttttcataat tttctaaaag
     2041 ataaaaatta ttaaaaagta ttattatatt tataatattt ttatataatt atcatagtta
     2101 aggataatat tgtattattt agtttcaata ttctattttt tttatattat caaattatag
     2161 taatactaaa tttctgttta gagaacctta tttaccatgt attttaactg cattattata
     2221 aatgggaata atacagtcct tgatatgcag agaatgcaaa aaagaatata aacctcaatt
     2281 tagatacatt tgtgaagatt gttttggacc tctggatgtt aaatatatct atcctaataa
     2341 tataaatcga gaattatttg gttctagaaa agaaaaaact tattggaggt attttgaact
     2401 cttaccaata agagataaaa ataacataat tagcattaac tcaggtttta ctcctttaca
     2461 aaaatctaca aatcttagta aggatttgca actaaaatct ttatacatta aaaatgattc
     2521 tgttaatcca accttctctt tcaaagacag acctgcaggt gttgctgtat ctagagcaaa
     2581 agaaattaat ctaaaagcag taggctgcgc ttcaacagga aatcttgcgt ctgctactgc
     2641 tgcgcatgct gcagtagcta gacttccttg ctatatattt gcacctgcgg atttagaatc
     2701 tgtaaaaatc tctcaggcat tagcctatgg tgcagagttt gttgctgtag aaggaactta
     2761 tgacgatgcc aatagggtag catcggtaat tggagactca aaaggaatag gtatagtgaa
     2821 tattaatatg agaccatact atgttgaagg atcaaaaacc cttgcttatg aagtagcaga
     2881 acaacttaat tggcaagtgc cagattatct agtaattcct gttggcagtg gtgcaatgtt
     2941 aaatgcaatt tgtaaaggtt ttgaggaatt atgtgaaata ggattgatta atgatgtttc
     3001 taatttaaaa attattgctg cacaacctca tgggtgttca ccgatagtag atgcatttaa
     3061 atcaaatagc aacgaaatat tccctgtaga aaaaccaaat actattgcaa aaagtcttgc
     3121 tataggtgat ccaggagacg gaatttatgt tttaaaaagg ttaaaacaat atcacggtat
     3181 tgctgaatca gctacaaatc aagaaatagt gaatggaata cttaaattat ctaagactga
     3241 aggtattttc acagaaccag ccggtggtgt atctgtagct gttcttcaaa aattaattga
     3301 aagcggtaaa atagatcgag atgcaaatat agtttgttat gttactggta atggtctcaa
     3361 atctattgaa gctatagtag gtatgctgcc aaaaccctct actgttaaac ccgatgttca
     3421 aatggtttct acaataatta gataagaaaa aataatatga ataatatatg tggaaataaa
     3481 aagtgaaggt ataaatggca aaaattgaat ttataattcc atctgtttta aataaaggtt
     3541 ctggagaaaa aaaaatatta ttagacgtac aagatttaca tgatgccttt aataaagcat
     3601 ctgataattt aggtgatgat tttaaacgta gagtattaga tcttaacggc aaacctcgag
     3661 ctcttataaa tatctatata aatggaaaaa atatgcgctt taacaaagat ggaatgacaa
     3721 catcacttaa agatggtgat tcaatctata tattgcctgc agttgctgga ggagcaaatg
     3781 aaacaaaaat tgaacatata gaacttagta gtcaagatct ccaacgttat tctagacaaa
     3841 taatgttaga agaaatagga tttattggga tggaaaaact tagaaactct aaagtttgtg
     3901 ttgttggagt cggtggaatt ggaaatccag ttgtaactca attaactgct atgggcgttg
     3961 gaaagatccg cattgtagat agagatgtag ttgaaatatc taatttacat cgtcaacatt
     4021 tgtatactga acaagatatt ggaaaagtca aagttgaagc agcaaaagaa catttgaaaa
     4081 ggaggaatac tgatgtagaa attgaagcgc ttcctatttc aattaccaat tacactgcag
     4141 aaaatataat aaaaggattt gatgttgtta tagatgcact tgatagtatt gatgcaagat
     4201 atgctttaaa tgatgcctgc attaagcaac atattccact catttatgca ggtgcattag
     4261 gaatgcttgg ttcagtatgc acaattcttc caaatcaatc tgcatgtctt cgttgtatat
     4321 ttcctgcgtt atctgaggat gatatgccga catgtagtac agaaggtgtt catccttcta
     4381 ttttgtattt agttggtggt atccaagtat ctgaagcgat aaaaatattg ttgggacaaa
     4441 aaccttcatt gataaatact ttattgtaca ttgatttaaa tgagctatca tttgaacgca
     4501 ttaaaatgtt aagacatgat gaatgctcag catgtggaaa agaaaaaata ataaaagaaa
     4561 tacaggtaga aaaagaacaa gatcttatta ttgaagaact atgtggtaga gatcgaggca
     4621 aaagaactta tactgtgaca ccctcaaaaa taacttctat agttaattta aataaagtaa
     4681 taaacaatgc agaattatta ggatataata taaaatcgag agggtcttta ggaataactg
     4741 taactactaa taatagtaat ccatcacaac aactatctat aagttttatg actagtggca
     4801 cagcaactat agtgggagca aaagacgaga atgctgctct tgctatttat aacgaatttt
     4861 taaattttaa aattagtaat aaaaaataaa ttattctaaa aataatttat ttatttttag
     4921 aatactactt taacagcctc ttttacttct ttcgatagtt ctctatagcc gtttttatct
     4981 attataacaa catttattcc atctcctgta ccagcatttc ttctaattgc tgctgctatt
     5041 gcctgcaaag ctaccatata tgcttcatct acactcatat tttctttaaa ttcactttct
     5101 aaataaccat aagctacagg agatccgcta cctgtagaaa taaatttttc ttcagttaat
     5161 gatccaaata aatcaatatt ataaacctga ccagtaccct tgccttcgta tcctgccatg
     5221 ataatctgaa cataatatgg aaaatatctt tgattaaaca aaatatttga acataatctg
     5281 gcagctgatt taacgggaat gattcttttg ttatttattc tatataggtt ggcattatat
     5341 cgcattgtat ctacaagatt ctgagcatca gctactcctc ctgcaatagt cattcctaaa
     5401 tgtttatcaa cattttgaat tttcattaca tgcttatcag ctataaacat acccgcacta
     5461 gcacgggtat cagcagctaa tacaacacca tcagaacatg aaagagcaca agtagttgtt
     5521 ccttttttaa tttgatttgc tatatactca gaatatctat tatcttgtac gtgcatctta
     5581 atcaaagtta atcagttcaa t
//
LOCUS       DATAFW010000117         3985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_242581, whole genome shotgun sequence.
ACCESSION   DATAFW010000117 DATAFW010000000
VERSION     DATAFW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242581"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..780
                     /gene="nuoB"
                     /locus_tag="VHJ57_02830"
     CDS             265..780
                     /gene="nuoB"
                     /locus_tag="VHJ57_02830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX2487498.1"
                     /translation="MIKDLINPDNLNLLVSKVSDVLVGALDQPLGYAINWGRIWSLWP
                     VHLETACCSVEFGATSSPRYDCERFGIIEAFGSLRQCDLIVVQGTVTRKMAPRLRMVY
                     DQMADPKYVIAMGACSITGGLYIDSYNVLPGIDGIIPVDVYVPGCPPRPETLIQGCML
                     LQEKIKRSKIK"
     gene            702..1307
                     /locus_tag="VHJ57_02835"
     CDS             702..1307
                     /locus_tag="VHJ57_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX2487499.1"
                     /translation="MSAKTRDTYSGLYVIARKNKEVKNKMTDINNDINQTTSNLNIVE
                     PDLKIEHSLYSQLSSEFKDKVRLVYTKPNRIKISVEPEHIIEVAFFLKNQLGLDHAES
                     VGGTDYPDENVIEVIYHLNSYSREELAPIILALSTKTDRNSSYLPSLLPVFKSVEYHE
                     RETYEMLGVYFQGHPRNERFLLPEDWADIPPLKKEFRIKGR"
     gene            1314..2489
                     /locus_tag="VHJ57_02840"
     CDS             1314..2489
                     /locus_tag="VHJ57_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX2487500.1"
                     /translation="MTNKYSESLDSKFKETTNLGLEVVKEASEDRLMKLSVGPQHPGS
                     GHFRFTIKVDGDYIVYCDPDPGYVHRGEEKMCEYRNYIQNIPHLERPVIHDSTNITYS
                     YSLAVEELLGIEVPRRGQFIRAIASELNRQVYTLYWLAIYGIFLGHSTMFMWPTGDRE
                     LFIDLLAALTGARVTHAFNIPGGVRNDIPEGFKEKCLEYIKYFEKRLKEYEDIFYNNP
                     LFRQRTENVGILPKQDAIDLGVTGSVLRASGIPHDVRKVEPYDVYNEIDFKVQYMKTC
                     DSFARAYVPFLDMRESCNITRQLLEKMPESGEVRVKLQSNPKGRIGEAYKRVESGRGA
                     LSYYIVSDGSSKPYRVKISVGSFRNILALPYLLVGSRLGDMPSIYWALNYWPVEADR"
     gene            2450..3751
                     /locus_tag="VHJ57_02845"
     CDS             2450..3751
                     /locus_tag="VHJ57_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX2487501.1"
                     /translation="MGSKLLACGGGQVEMTTAPKDFRFGSFVGSIVWLLFWIIVIVSL
                     VGLPLIFIILFYIPLPYSNGEVITPYLFLSMIVDPTRTIPIIKFLIHTDFFRAAVFPG
                     FMYAALIAAATIFVERKFLAKMQLRVGPLYAGKIEGILQLMADGLKLMSKEIIAPSGA
                     DKPIFWTTPILFVATAAALTALIPVAPGWVVSDLDVGLLAIFAILGFFPLIALLFSWS
                     CNSKYPFIGGLRALHQMIAFEIPFILSALSIVLLSASLNLTDIVEAQYAFWFFAIAPI
                     SAFVFYISSLAELERVPFDLPEAESEIVAGWLTETSGMIYGLIQLGTYLKLYVLAALF
                     VILFMGGWMGPQIFPQELIPAFTGEKIYNATVVNGIFWFTIKTFGMIMLILLPRGINP
                     RIRIDILLHTGWYKLIVLSFINMFIVLAMIYGGVITPGGLQ"
     gene            3757..>3985
                     /locus_tag="VHJ57_02850"
     CDS             3757..>3985
                     /locus_tag="VHJ57_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487502.1"
                     /translation="MSSSIIKKTSTATGFIKAIESGSKHLLMKRFTLRFPEQKLKFVG
                     DGYQFDPKRGVGIAGSRGRHILFHEKCTGCQL"
BASE COUNT         1250 a          610 c          784 g         1341 t
ORIGIN      
        1 atgcgatatt tttattgaat ggcataatat ctggtgtccg tgttgtgggt atagattaag
       61 ggtaaatcca agaaacggga aatttaagac aatagtgtga aacactagaa atttacagtt
      121 aatatactac ttaaattagt agatttttca atttattaaa aaacaatagt agaaaataag
      181 ttgatagtgc caaagaatag aaacaagaac ataatagatt aaataccttt gttgtatatt
      241 tattaataaa ggaggtcgat gttaatgatt aaggatctta ttaatccaga taacttgaac
      301 ttgttggtaa gtaaagttag tgatgtttta gtaggtgctt tggatcaacc attaggttat
      361 gcaattaatt ggggaagaat ctggtctcta tggcctgtcc accttgaaac tgcatgttgt
      421 agtgtagaat ttggtgctac atctagtcct cgttatgatt gtgaaaggtt cggcattatc
      481 gaagcttttg gttccctacg acagtgtgat ttaatagttg ttcaaggtac agttactaga
      541 aagatggctc cgagattgag aatggtatat gatcaaatgg cagatcccaa atatgttatt
      601 gcaatgggag catgttctat tactggagga ctgtatattg attcttataa tgttttacct
      661 ggaatagatg gtattattcc tgtagatgtc tatgtaccag gatgtccgcc aagaccagag
      721 acacttattc agggttgtat gttattgcaa gaaaaaataa agaggtcaaa aataaaatga
      781 ctgatattaa taatgatatt aatcagacta cttcaaattt gaatattgtt gaaccagatt
      841 taaagatcga acattcatta tattctcaat tatcctcaga attcaaagat aaagttcgat
      901 tagtatatac aaaacccaat cgcataaaaa tttctgtaga accagaacat attattgagg
      961 ttgccttttt cttgaaaaat caacttggtc tagatcatgc agaatctgta ggtggaactg
     1021 attatcctga tgagaatgtc attgaagtga tttatcattt aaattcttat tctcgtgaag
     1081 aactcgctcc tattatccta gcgctttcta caaaaactga tagaaattct tcctacttac
     1141 cctccttact tccagtattt aaaagcgtag aatatcatga aagagaaaca tatgaaatgt
     1201 tgggtgttta tttccaaggc catcctagaa atgaaagatt tcttctacca gaagattggg
     1261 ctgacattcc tccattaaaa aaagaattta gaattaaagg taggtagaat ggtttgacaa
     1321 acaaatatag tgaatcattg gatagtaaat ttaaagaaac taccaacttg ggattagaag
     1381 tagttaaaga agcgtcagag gacagattaa tgaaattgag tgtaggtcct caacatccag
     1441 gttctggaca ctttagattt acaataaaag ttgatgggga ttatattgta tattgtgatc
     1501 ctgatccagg ctatgttcac agaggagagg aaaaaatgtg cgaataccga aactatatcc
     1561 aaaatattcc tcaccttgag cgacctgtaa tccatgattc tacaaatatt acctattcat
     1621 atagcttggc agtagaagaa ttgcttggaa tcgaagtacc aagacgaggg caattcattc
     1681 gggctattgc atctgaactg aatcgtcagg tttatacatt atattggctt gcaatttatg
     1741 gtatattttt aggacactcc acaatgttta tgtggcctac tggtgataga gaattattta
     1801 ttgatctact tgcagcactt accggtgcga gagtaacaca tgccttcaat attccaggag
     1861 gtgtaagaaa tgatataccg gagggattta aagaaaaatg tttagaatat ataaagtatt
     1921 ttgaaaaaag attaaaagag tacgaggata ttttttataa taatcctttg tttagacaaa
     1981 gaacagaaaa tgttggcatt cttcctaagc aagatgcgat agacttgggt gttacagggt
     2041 cagtcttacg agcttcaggc attcctcacg atgttcgaaa agttgaacca tatgatgttt
     2101 ataatgagat tgacttcaaa gtgcaatata tgaaaacttg tgattctttt gctagagctt
     2161 atgttccatt tcttgacatg cgtgaatctt gtaatattac tcgtcaattg ctagaaaaga
     2221 tgcctgaatc aggcgaagta agagtgaaat tacaatcgaa tcctaagggt cgtattggtg
     2281 aagcttataa acgagttgag tcaggaagag gtgcattatc ctattatata gttagtgatg
     2341 gttcatctaa accttacaga gtaaaaattt cagttggatc ttttaggaac attttggctt
     2401 tgccttattt actggtaggt tcacgactag gagatatgcc atctatatat tgggctctaa
     2461 actattggcc tgtggaggcg gacaggtaga gatgacgaca gctccgaaag attttagatt
     2521 tggtagtttt gttgggtcaa ttgtgtggtt attattttgg atcatcgtaa tagtttcact
     2581 agttggcctg cctttaattt ttattatttt attttatatt cctcttcctt attctaatgg
     2641 ggaagtaatt accccatatc tctttttgtc aatgattgtg gatcctactc gtacaattcc
     2701 aataattaaa tttcttattc ataccgattt cttcagagct gctgtattcc ctggatttat
     2761 gtacgctgct ttgatagcag cagcaactat ttttgtagaa agaaaattcc tcgctaaaat
     2821 gcaattaaga gtaggtccgt tatatgcagg aaaaattgaa gggatattac aattaatggc
     2881 tgatgggtta aaattaatgt caaaggagat tattgctccc tccggagccg ataaacctat
     2941 cttctggact actccaattc tttttgtagc cactgccgct gctttaactg ctcttatccc
     3001 agtagctcca ggttgggttg tatccgatct agatgtcggc cttcttgcca tttttgcaat
     3061 tttaggtttt tttccattaa ttgcactact tttttcttgg tcttgtaata gtaaatatcc
     3121 cttcatcggt ggtctcagag ctttgcatca aatgattgcc tttgagattc catttatttt
     3181 atctgcgcta tctatcgtgc ttcttagtgc ctctttaaat ttgacagata ttgtggaagc
     3241 gcaatatgcg ttttggtttt tcgctattgc tccaataagt gcatttgtat tctacatttc
     3301 atctttagcc gaattggaac gagtcccttt tgatttaccg gaagcagaaa gtgaaatagt
     3361 tgctggatgg ctcacagaaa cctcaggaat gatttatgga ttgatacagt taggaactta
     3421 tctcaagttg tatgttttag cagctttatt tgttattctc tttatgggag gatggatggg
     3481 tccacaaata tttccacaag aattaattcc tgcatttaca ggtgaaaaaa tatacaatgc
     3541 tactgttgtc aatgggattt tttggtttac aattaagact tttggtatga taatgttaat
     3601 attgcttcct agagggatta atcctcgaat cagaatcgat atccttttgc atacaggatg
     3661 gtacaaatta atagtcctct cttttatcaa tatgtttatc gtcttggcaa tgatatatgg
     3721 tggagtaatt actccaggag gcttacagta aatataatgt catcatcaat tattaaaaaa
     3781 acatcaacag caactggatt tattaaagct atagaatcag gttccaaaca ccttctaatg
     3841 aaaagattta cgttacgatt tccagagcaa aaattgaagt ttgtaggaga tggataccaa
     3901 tttgacccaa agagaggggt aggaatcgca ggaagtagag gacgacatat cctctttcat
     3961 gagaaatgta ctggatgtca acttt
//
LOCUS       DATAFW010000118         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5884748, whole genome shotgun sequence.
ACCESSION   DATAFW010000118 DATAFW010000000
VERSION     DATAFW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5884748"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VHJ57_02855"
     rRNA            <1..161
                     /locus_tag="VHJ57_02855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            complement(303..1271)
                     /locus_tag="VHJ57_02860"
     CDS             complement(303..1271)
                     /locus_tag="VHJ57_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX2487503.1"
                     /translation="MNSLETLEHFFVAPEKTNSKCSFGDFPTKITFKESDAILYGVPL
                     DITTTFGKGTSYGPEAIRVTSSKQIESFVLDENQEIYEKIKIYDIGDLSLVNSKINSK
                     YEKLLENNLKRISKINKINSLIRKEKKVPIILGGEHTLSYFSIKSLAHENPIIIHFDA
                     HRDMKPMYDRKKICHTTPFFHLIDEGHITGDNIIQLGIRQSDKEENDMATNNRVNTFT
                     GWEINKDIHKVTRKIRELTNNRNIYITFDIDVYDICYVPCTGTPEPFGLNPFQIIEIL
                     KSINKSAVVIGIDMVEVAVKNNDFREGTLATQTLYRIFSNLYKKSM"
     gene            complement(1335..>1752)
                     /locus_tag="VHJ57_02865"
     CDS             complement(1335..>1752)
                     /locus_tag="VHJ57_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487504.1"
                     /translation="ETGKIHPSELSFNVMVNKSPDRYGEKTNNSKVKSIDGKNIDFIT
                     YKGLPQHIKAAMLLHQSGREIKAGDIISYVKTKTNDGVKPVDLLRNINEIDTEKYLEA
                     MESTFDQLLSALNYNFQSILGKDRQSNLDELFWSKK"
BASE COUNT          532 a          269 c          259 g          692 t
ORIGIN      
        1 tcctgaaagc atctaaggag gaagccattc tcgagaaaag acagccttcc gtaaggagaa
       61 gggtagctat agaagatagc attgatggaa tggaggtgta cgcatcaagc ttcggcgaga
      121 tgttcagcct gccattacca ataacccaag gcacctgata tatttactct ttaatcttgg
      181 ttagtatacg taaaggttag agtactattg gataattaga tcctatacac gatgaggttt
      241 tttattataa tatttatagt ctatttatat taattaataa actatctttt ttatgtgtca
      301 atttacattg attttttata gaggtttgaa aaaattctgt aaagtgtttg agtggctaaa
      361 gttccctctc taaaatcatt attcttaact gcaacttcta ccatatctat tccaatcact
      421 acagcacttt tattgatcga tttaagtatt tctattatct ggaaaggatt taatccaaat
      481 ggttcaggtg ttcctgtaca aggtacataa caaatatcat atacatcaat atcaaatgta
      541 atgtatatgt ttctattatt tgtaagttcc cttatttttc ttgtgacttt atgaatatct
      601 ttattaattt cccatcctgt aaaagtatta acacgattgt tggtagccat atcattctcc
      661 tctttgtcac tttgtcgaat tccaagttga ataatattat cacctgtgat atgaccttca
      721 tcaatgagat gaaaaaacgg tgttgtatga caaattttct ttctatcata cattggtttc
      781 atatctctgt gtgcatcaaa atgaattatt ataggattct catgagcaag tgattttata
      841 ctaaaatatg ataatgtatg ctctcctcct aagataatag ggactttttt ttcttttctt
      901 attaatgaat ttattttatt tattttggaa attcttttca agttattctc taaaagtttt
      961 tcgtatttac tatttatttt agaattgact agggataggt cacctatatc atatatttta
     1021 attttttcat aaatttcttg gttttcatct agtacaaaag attctatctg ttttgaagac
     1081 gttactctaa ttgcttcagg gccataagaa gttcctttac caaaagtagt agttatgtcc
     1141 aaaggaactc catataaaat tgcatcagat tctttaaatg ttattttagt tggaaaatct
     1201 ccaaaactac attttgaatt agttttctca ggagcgacaa aaaagtgctc taaggtttct
     1261 aaagaattca attatcttaa tttaggaagc tacaaattaa attgtttcta gattctccat
     1321 aaccaatgtt tttcttattt tttagaccaa aatagttcat ctaaattaga ttgtctatct
     1381 tttcctaata tggattggaa gttatagttt aatgcagata ataattgatc aaaagttgat
     1441 tccatagctt caagatattt ttctgtgtcg atctcgttaa tatttctaag caaatctaca
     1501 ggttttaccc catcgttagt ttttgttttt acataggaga ttatgtctcc tgctttaatc
     1561 tcccttcctg actgatgaag taacattgca gctttgatat gttggggtag gcctttgtat
     1621 gttatgaaat caatattttt tccatctatt gattttacct tagaattatt tgtcttttct
     1681 ccatatcgat caggtgattt gttaaccata acgttaaaac tcaattctga aggatgaatt
     1741 ttaccagtct ct
//
LOCUS       DATAFW010000119         5555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4826169, whole genome shotgun sequence.
ACCESSION   DATAFW010000119 DATAFW010000000
VERSION     DATAFW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4826169"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..517
                     /locus_tag="VHJ57_02870"
     CDS             41..517
                     /locus_tag="VHJ57_02870"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX2487505.1"
                     /translation="MEKLNLVATGGTFDYIHIGHIKLLSTAFDISHHVIIGVTSDEFV
                     KRFKVPSSIKNNYLRRLSNLKNNIAVNFKDVNYTIIKLEDEFGPVIHSSEIQAIVVSE
                     ETLVKVKEINEIRIKNHLHPLRVIVVAIARSSDGKPISSTRIRNGEIDDKGKILRK"
     gene            complement(995..1294)
                     /locus_tag="VHJ57_02875"
     CDS             complement(995..1294)
                     /locus_tag="VHJ57_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487506.1"
                     /translation="MRKSHNIICSDKYEKEKLTGFFSIQKDDSVFEMDVENLIENEII
                     VKLKDKSCHSITLKNKDNANTLFILIKEIKNGKRRFVSLDNLNSNTEIQIITETI"
     gene            complement(1291..2481)
                     /locus_tag="VHJ57_02880"
     CDS             complement(1291..2481)
                     /locus_tag="VHJ57_02880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX2487507.1"
                     /translation="MEGPPHTIDTNNINNSHDNYKDIVVTPWEVFGDLEDKMYIKLIE
                     KFGTQAITHDLIEKFKKLTGEIHPLIKSQYFFSHRDLDWLLTKYENGDPFYLYTGRGP
                     SGFVHLGHILPWLFTKYLQDKFGSKLLFQLTDDEKFLYNPGATRETISEYTSENILDI
                     IAVGFNPNLTRIIIDTKHINYLYPLATEIAKKITFSTAKAVFGFTNTTNIGMIGFPPI
                     QAAPCFLPSLIEKKPTPVLIPAAIDQDPYWRITRDIAAKLGFYKPAQIHSKFLPSLAR
                     ISKMSSSRPETAIFTTDEPEIVEKKVLSSFTGGQATVSLQKKLGGNADICPVYGYLKY
                     FFDNEKESLERYIRCKSGNLLCGECKSDLANNTKTFIIDFKHKREKAKDKVKDFMFDE
                     KFSS"
     gene            2543..4078
                     /locus_tag="VHJ57_02885"
     CDS             2543..4078
                     /locus_tag="VHJ57_02885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX2487508.1"
                     /translation="MENTQINLAELHPIERKIISSLNPLSQISLTDLIELSALNLDQI
                     RRGLEWLKYKNIIESNKPSTLITLDDLGRNAIEQGLPERRLVRAVRNGNITISTILEK
                     GSLTQSEINIAISKAKSNKWINISSSETEGLTFNLIENFENASGEELLLNKIFSSKKL
                     KEVDLTNSELEFLKLLVKRPKFIKFKKDEEKVSLTELGKKIKTKVDIKNINDKNFPLN
                     KNTTLLVPKHLIAGKWKTLTFRPIDVEAKVAMANPGRKHPLTDLINEIREAFTSLGFV
                     EIDGDFIQSAFWNFDALFTPQDHAARDMQDTFYLSEKITTKLGNKNLIKKIANTHILG
                     WKDSWNIKIAKKPVLRTHTTPVTLKYLSETKPDEARIFTIGRVFRNEKVSYKHLVEFN
                     QIEGVITGKKLTLRDLMGIQTEFYKKIGIKKIKFWPTYFPYTEPSLQSMIFNSNLNKW
                     IELFGMGILRPEVVQPFGIRNIVLAWGGGIERIAMLKYEISDVRELYNNKIGWLRSLS
                     KCQ"
     gene            4069..>5555
                     /gene="pheT"
                     /locus_tag="VHJ57_02890"
     CDS             4069..>5555
                     /gene="pheT"
                     /locus_tag="VHJ57_02890"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX2487509.1"
                     /translation="MPVVNIFKTRIKSVFPNKQIEEILGKLPFVGLDIEGVDKSKIRV
                     EYNPNRPDFSSDTGIFRSLKGLLNNTLGLPSFRYSSDKSYQITIDRKVKQIRPILLFF
                     VAKNTNINNFILNQLISMQEDLHNGIARGRKKAAIGIHDLNQIEFPLIYNTKSQQESF
                     IPLDQTQPFRLKEILSDLEVGKKYSSLLKNFNLYPTIADRNNQILSFPPIINSSKTKI
                     NDNTSNLVIEITGTNLETCKSILAIIAYYLYDEGYEIFSGIVHDDKSAFSSLTLLKDN
                     TISVDPNIINSYLGLDLSIKKLIQILNRCRIHASIINNKKIKCLVPPYRNDIISFIDI
                     VEEVAIGYGIDNIKSTIPSFLITSGVKNHYFKKMDEIKETLTGLGFLEMMNFTMLDRT
                     ISSIFNFEDKELLEKIKVKESKSKEHEFLRTNILSSLVNNLSYNVHEDYPQKVFEIGK
                     TFSFKNKIVEQWNLGVLIASNNSNYTEAKSIAESIFKINFGKNLIT"
BASE COUNT         2098 a          737 c          856 g         1864 t
ORIGIN      
        1 ctatttttct tttattgata aattatttag ataaagacga ttggaaaaat taaatcttgt
       61 cgcaacgggt ggaacttttg actatattca tattggtcat ataaaacttc tttcaactgc
      121 ttttgatatc agccaccatg tcattattgg agtcacaagt gatgaatttg taaaaagatt
      181 taaggtacct tcgagtataa aaaataatta tctaaggaga ctttcaaatt taaaaaacaa
      241 tattgcggta aattttaaag atgttaatta cacaataatt aaactagaag atgaatttgg
      301 ccctgttatt cattcctcag aaattcaagc aattgtagtt agcgaagaaa cattggtgaa
      361 agtaaaggaa attaatgaaa tcagaattaa gaaccatctt catccattac gggttatagt
      421 agttgccata gcaagatcta gtgatggaaa gcctatatca tctactcgaa taagaaatgg
      481 tgaaattgat gataaaggaa aaattctaag aaaataataa agagaataat tttttttagt
      541 gataaaatct attttactaa tatttagagt tcaatgtatt ttgataattc agttaattta
      601 gattttgata aaattgataa atgtattcga aacttcttat ggtcctgggc gagagatgaa
      661 ctatgaatct attgaaaaag taggaaggga tttaaaggat aaaattcatt tcgaacatga
      721 atagattcta tgaataaagt gtaaaatcaa tatttccttc cttgcaactc ctatagtttt
      781 agaaacaata gaacgcttta aaccattaat ggaatattct cccaaattat atttagaaaa
      841 attcacgaaa ttttgaaaaa atctagaata taggtaatta aaaaaagaat aaaaaaatta
      901 taagatacaa aaaatataaa actagtaatt ataacccaaa aaggtataaa tcaagtagaa
      961 aaagattttt ttctttaata ttaaaacaaa attgttaaat tgtttctgtt attatttgaa
     1021 tttcggtatt agaatttaaa ttgtctagag aaacaaatct tcttttacca ttttttattt
     1081 ctttgatcaa tataaacaga gtatttgcgt tatctttatt ctttaaagta atactatgac
     1141 aagatttatc ttttaattta actattattt cattttcgat taaattttca acatccatct
     1201 caaaaactga atcatctttt tgtatcgaaa agaagcctgt caatttctcc ttttcatatt
     1261 tatcagaaca tataatgttg tgtgattttc tcatgatgaa aatttttcgt caaacataaa
     1321 atcttttacc ttgtctttag ctttttctct tttatgttta aagtcaatta tgaatgtttt
     1381 agtattattg gcaagatcac ttttacattc tccacaaagt aaatttccac ttttgcatct
     1441 aatgtagcgt tccaaagatt ctttctcatt atcaaaaaag tattttaagt atccgtatac
     1501 tggacatata tcagcatttc ctcctaactt tttttgtaat gaaactgtgg cctgcccgcc
     1561 tgtaaaggaa gataatactt ttttttcaac aatttccggt tcatctgttg taaaaattgc
     1621 cgtttctggt cttgaagatg acatttttga aattcgtgcc aaacttggta aaaatttact
     1681 atgtatttga gcaggtttgt aaaaccctag ttttgcagct atatctcttg ttattctcca
     1741 gtaggggtct tggtcaattg ctgcaggaat tagaactggg gttggttttt tttcaattag
     1801 agaaggaaga aaacaaggag cagcttgaat agggggaaaa ccaatcatac ctatgtttgt
     1861 tgtatttgta aaaccaaaaa ctgcttttgc ggtagaaaaa gttatttttt ttgcaatctc
     1921 tgttgcaagt ggatataaat aattaatatg tttcgtgtct ataattatcc tcgttaagtt
     1981 aggattgaag cctactgcta taatgtcaag tatattttct gaagtatact cagaaatggt
     2041 ttctctagtt gctccgggat tataaagaaa tttctcatca tcagttaact gaaataataa
     2101 tttagagcca aatttatcct gtaggtattt cgtaaataac caaggcaaaa tgtggccgag
     2161 atgtacaaaa cctgatgggc ctcttccagt ataaagataa aaagggtcac cattctcata
     2221 tttcgttagt aaccaatcaa gatctctatg tgagaagaaa tattgagatt ttataagtgg
     2281 atgaatttct cctgttaatt ttttaaattt ttcaattaaa tcgtgtgtga tagcctgtgt
     2341 tccaaatttc tctattagtt taatatacat tttatcttct aaatcaccaa aaacctccca
     2401 tggtgtaaca acaatatctt tataattatc atgtgaatta ttgatgttat tggtatctat
     2461 agtatgtgga gggccttcca ttttcttaaa tagtggaatt acaaacgttt aaacgtttaa
     2521 aaataatttt taataaactt aattggaaaa cacccaaatt aatctcgcag aattacatcc
     2581 aatcgaaaga aaaattatta gttcgttaaa tcctttatct caaatttctt taactgattt
     2641 gatagaattg agtgcgctta atcttgacca aattagaaga ggattagaat ggttaaaata
     2701 taaaaatata atcgaatcaa acaaaccttc tactttaatc acattagatg atttaggtag
     2761 aaatgccata gaacagggtc tacctgaaag aagattagta agagctgtaa gaaatggcaa
     2821 tataactatt agcacaatat tagaaaaagg atcgttgacg caaagtgaaa ttaatatagc
     2881 aatttcgaag gctaaatcta ataaatggat aaatatatca agttctgaaa cagaaggatt
     2941 aacttttaac ctaatagaaa attttgaaaa tgcaagtggt gaggagttgt tattgaacaa
     3001 aattttttca tctaaaaaac taaaagaggt agatttaacg aattctgagt tagagtttct
     3061 taagttacta gtaaaaagac ctaaatttat taagtttaaa aaagatgagg aaaaagtatc
     3121 acttactgaa ctaggaaaga agatcaagac taaagtagat ataaaaaata taaatgataa
     3181 gaacttccca ttaaataaaa atactactct actagttcca aagcatttaa tagcaggaaa
     3241 atggaagact ttgacgtttc gtcccattga cgtagaagcc aaagttgcca tggcaaatcc
     3301 aggaagaaaa catcctttga cagatttgat taatgagatt cgtgaagcct tcacaagtct
     3361 tggatttgta gaaattgatg gtgattttat acaatcagcc ttttggaatt ttgatgcgct
     3421 ctttacccct caagatcatg ctgctaggga tatgcaggat accttttatt tatcagagaa
     3481 aatcacaacc aaattaggaa ataaaaacct tataaaaaaa attgctaata cacatatact
     3541 tggatggaag gattcttgga atatcaaaat agcaaaaaaa cctgtgttac gtacccatac
     3601 gacgccagtg acattgaaat atctgtctga aactaaacca gatgaagcta gaatatttac
     3661 aattggacgt gttttcagaa acgaaaaagt ttcatataaa catctagtgg aatttaatca
     3721 aattgaggga gtcataacag gaaagaaact aactttaaga gacctaatgg gtattcaaac
     3781 ggaattctat aagaagattg gaattaaaaa aattaaattt tggccaactt attttccata
     3841 taccgaacca tcactacagt caatgatctt caacagtaat ttaaataaat ggattgagtt
     3901 atttgggatg ggcatacttc ggccagaagt agtacaacca tttggtataa gaaatattgt
     3961 tttagcatgg ggaggaggca tcgagagaat agcgatgtta aagtatgaaa tttcagatgt
     4021 aagagaatta tataataata aaattggatg gttacggagt ttaagtaaat gccagtagta
     4081 aatatcttta agacacgtat taaaagtgtt tttcccaata aacaaattga agaaatatta
     4141 ggaaagcttc catttgttgg attagatatt gaaggagtag ataaatcaaa aataagagta
     4201 gaatataatc ctaatcgtcc tgacttttca agtgatactg gtatatttag atctctaaag
     4261 ggattattaa ataatacatt aggtttacct tcattcagat attcttcaga taaatcatat
     4321 caaataacta tagatcggaa ggtaaagcaa ataagaccaa tactattgtt ttttgttgct
     4381 aaaaatacta acataaataa ttttattctc aatcaattaa tttctatgca agaagatctt
     4441 cataatggga tagccagagg cagaaaaaaa gctgcaatag gtattcatga tcttaatcag
     4501 attgaatttc cattaatcta taatacaaaa tctcaacagg aatcatttat accattggat
     4561 caaactcaac catttagatt aaaagaaata ttgtctgatc tcgaggttgg aaaaaaatat
     4621 agtagtttat taaaaaattt taatctatat cctactatcg ctgatagaaa taatcaaatt
     4681 ctatcatttc cccctatcat aaatagtagt aagaccaaaa taaatgacaa tactagtaat
     4741 ttagtaatag aaataacagg tacaaattta gaaacatgta agagtatttt agctatcatt
     4801 gcatattatc tctatgatga aggttatgaa attttttctg gaatcgtaca tgacgataaa
     4861 tctgcatttt cttcattgac acttcttaaa gataatacta tttcagttga tccaaatatt
     4921 attaatagct atttaggatt agacctatca attaaaaagc ttatacaaat tttaaataga
     4981 tgtcgaatac atgcttcaat aataaacaat aaaaaaatta aatgtctagt tcctccttat
     5041 aggaacgata ttatcagctt tatagatatt gtcgaagaag tggcaatagg gtatgggatt
     5101 gataatatta aatctactat accgtcattt ttaatcactt caggggtaaa aaatcactat
     5161 tttaaaaaaa tggatgagat aaaggagaca ctgactgggc tcggattttt agagatgatg
     5221 aattttacca tgttagatcg aaccatttct agtatattta atttcgagga taaagaattg
     5281 cttgaaaaaa ttaaggtaaa agaaagcaaa agtaaagaac atgaatttct tagaaccaat
     5341 atattatcat ctttggtaaa taatctttcc tacaatgttc atgaggatta ccctcagaaa
     5401 gtatttgaaa taggaaaaac atttagtttt aaaaataaaa tagtagaaca atggaattta
     5461 ggcgttctaa ttgcatctaa caattctaac tatacagaag ctaaatcgat tgcagaatct
     5521 atttttaaaa taaattttgg taaaaatctt ataac
//
LOCUS       DATAFW010000120         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6238818, whole genome shotgun sequence.
ACCESSION   DATAFW010000120 DATAFW010000000
VERSION     DATAFW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6238818"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /gene="uvrA"
                     /locus_tag="VHJ57_02895"
     CDS             complement(<1..2487)
                     /gene="uvrA"
                     /locus_tag="VHJ57_02895"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX2487510.1"
                     /translation="MIDKLSVRGAKQHNLNNINIDIPKNKIVVFTGLSGSGKSTLAFD
                     TIYAEGQRRYVESLSAYARQFLELMEKPDVDSIEGLSPAISIQQKTTNKNPRSTVGTV
                     TEIYDYLRLLFAKIGEPYCPNCNKKIKSQTLESITESVLRFSHEKSLLLILAPLIQGK
                     KGTFDRLFPELYEKGYSRLRIDGQFVDLDEFIITSTDEQYNDKNNRRTADFKDINLEK
                     QKKHNIDVIIDRLKPSLNEKSRIFESVQNAANLTNGIILTIIDDKEFLFSRHNACPFC
                     KISIGNIEPRSFSFNSPFGACEECNGLGIKIEFDPDLIIPDRNKSIIEGAIKPWQGSF
                     ATFRSSMLKYVGKRFGFNLNTPIQKMSSEQINVILYGTNQNIYYKYESKYSQSKWEYE
                     SNFEGVIPNLQRLYNSTDSENKREMLARFMKEQVCDKCSGKRLKKDSLSIKLSEKSIM
                     DVGNLPIDECYNFFNRLNLDDTKKYIAKNVLKEIRSRLEFLINVGLNYLTLNRSTSSL
                     SGGESQRIRLATQIGSNLTGVLYVLDEPTIGLHQRDNDRLINTLKRLRDLGNSLIIVE
                     HDEDVIRNSDWIIDIGPGAGIHGGRIVAQGNLDQIVQNENSLTGKYLKGEKTATLFSK
                     KNQNANLLTIHGAKENNLKSIDVDIPLGCMTIVTGVSGSGKSTLINEILFKSVSNYFG
                     TNVIPGKHDSISGMDNIDKVIDIDQSPIGRTPRSNAATYVNAFTPIRELFSKTMTAKE
                     MGYNPGRFSFNVKIGRCEACEGCGVKRIEMQFLPDVYVKCNECLGKRYNSETLLVKYK
                     GKTIDDILNLTVEEAMDFFQNVPIIYKKLKT"
     gene            complement(2484..>3703)
                     /locus_tag="VHJ57_02900"
     CDS             complement(2484..>3703)
                     /locus_tag="VHJ57_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX2487511.1"
                     /translation="TDKKSRENAIRMIKKELKGWLPKLEGEIEKERLASRTKYDIEMI
                     NELGYCSGIENYSRHFDGRKEGEPPFCLLDFFGDDFLLVIDESHVTLPQLRGMYNGDH
                     TRKEMLVTYGFRLPSAFDNRPLKFEEFERYLRKVIFVSATPGPYELNSSFQVVEQLVR
                     PTGLVDPIVEIRSSKNQIDELIFEIQNRVSKNQRTLVTTLTKRMAEDLAEFLSKKNIR
                     VRYLHSEIEGLERTELIRQLRLGEFDVLIGINLLREGLDIPEVSLVVILDSDKEGFLR
                     NDTSLIQTFGRAARNIDGTVIMYGDKVTKSMENALAETNRRRKKQIEFNNLNKITPIS
                     ILKPIITNKNSDTQHEFFKSMSKNDLRKLSVETEALMNKLAEELEFEKAIQLRDKLKK
                     INQEIGILEVARL"
BASE COUNT         1075 a          674 c          513 g         1441 t
ORIGIN      
        1 agtttttaat tttttgtaaa taataggtac attttgaaag aagtccatag cttcttctac
       61 tgtcaaattt aatatatcat ctatagtctt acccttgtat tttactaaca atgtttcaga
      121 attataacgc tttcccaaac attcattgca ttttacatat acatcgggta aaaactgcat
      181 ttcaattcgc tttactccac atccttcaca tgcctcacac cttccaattt ttacgttaaa
      241 ggaaaatctt ccaggattat aacccatctc cttggcagtc atggtcttag aaaaaagctc
      301 tctaatggga gtaaaagcat taacatatgt agctgcatta gatcgaggag ttctaccaat
      361 aggagattga tcaatatcaa taaccttatc aatattatcc ataccactta tggaatcatg
      421 ttttccaggt atgacattgg ttccaaaata attagacacg gacttgaaaa gaatttcatt
      481 gataagtgtc gatttacccg acccagaaac tccggtaact attgtcatac acccaagagg
      541 tatatctaca tctattgatt ttagattatt ctcttttgcg ccgtgtatag taagtaaatt
      601 agcgttttga tttttttttg aaaagagagt cgctgttttc tctcccttta aatattttcc
      661 tgtcaatgaa ttttcgttct gtacgatttg gtccaaattt ccttgagcta ctatccttcc
      721 accatgtatc ccagctcctg gaccaatatc gataatccaa tctgaatttc ttattacatc
      781 ttcgtcatgc tcaacaatta taagagaatt gcccaaatct cgtagtcttt ttagagtatt
      841 gattaatcta tcattatctc tttgatggag tcctattgtt ggttcatcta gaacgtagag
      901 tacaccagtt aagtttgagc caatttgagt agcaagtcta attctttgag attctcctcc
      961 agatagtgaa gaggttgatc ggtttaatgt taaataattt aatcctacat taattaaaaa
     1021 ttctagtcga gatctgattt ccttcaacac attttttgca atatattttt ttgtatcatc
     1081 aagatttaat ctattgaaaa aattatagca ttcgtcaata ggtaaatttc ctacgtccat
     1141 tattgatttc tccgatagtt taatagacaa tgaatctttc tttagcctct ttccagaaca
     1201 tttatcgcaa acctgttcct tcataaatcg tgcaagcatt tctcttttgt tttctgagtc
     1261 tgtactatta tacaatcttt gtagattggg gattactcct tcgaaattac tttcatattc
     1321 ccacttgctt tgtgagtatt ttgattcata tttatagtaa atattttgat ttgttccata
     1381 taatataaca tttatttgtt cagagctcat tttttgtatt ggagtgttta agttaaatcc
     1441 aaatcttttt ccaacatatt ttaacataga tgatctaaaa gtcgcaaaac ttccttgcca
     1501 aggtttaatt gctccctcaa taatactttt gtttctatct ggaattatta aatcaggatc
     1561 gaattctatt ttgataccta aaccattaca ctcttcacat gctccaaaag gagagttaaa
     1621 cgagaaagat ctgggttcaa tatttcctat gcttattttg cagaaaggac aagcattgtg
     1681 cctagaaaat aaaaactctt tatcatcaat tattgtaaga attattccat tagttaagtt
     1741 tgctgcattt tgtactgact cgaaaattct acttttttca tttaatgagg gttttaatct
     1801 gtcgattatc acatcaatat tatgtttctt ttgtttctct aaattaatgt ctttaaaatc
     1861 tgctgttctt ctattatttt tatcattgta ttgctcatcc gtagaggtaa taatgaattc
     1921 gtctagatca acaaattgtc catcgattct taacctcgaa tatccttttt catataattc
     1981 cggaaataat ctatcaaaag tacctttttt tccttgaatt aatggggcga gaataagtaa
     2041 gagggatttt tcatgagaaa accttaatac tgattcggta attgattcta atgtttgact
     2101 ctttatcttt ttattacaat ttggacaata aggttcgcca atttttgcaa acaatagtct
     2161 tagataatca taaatctcgg ttactgttcc tactgttgat cggggattct tatttgttgt
     2221 tttttgctgt attgaaatgg ctggagataa tccttcaata gaatctacgt caggcttttc
     2281 cataagttca agaaattgtc tggcataagc tgaaagtgac tcaacatatc ttctctgccc
     2341 ttctgcataa atagtatcga atgctagtgt ggatttaccc gatccagaaa gacctgtaaa
     2401 cactacaatc ttatttttgg gtatatcaat gttgatattg tttaagttgt gttgtttcgc
     2461 acctctaaca cttaacttgt ctatcataac ctagccacct ctaatattcc aatttcttga
     2521 tttattttct ttaatttatc tcgcagttgt atagctttct cgaattcaag ttcttcagcc
     2581 aatttattca tcaatgcttc ggtttcaact gatagctttc tcaagtcatt tttactcata
     2641 gatttaaaaa attcatgctg agtatcggaa tttttatttg taattattgg ttttaatatt
     2701 gaaataggag ttattttatt taagttatta aattctattt gtttttttcg tcttctattt
     2761 gtttctgcta aagcattttc cattgatttt gtaactttat ctccatacat tattacggtt
     2821 ccatcaatat ttctagcagc tcttccaaat gtttgtataa ggctagtatc atttcttaaa
     2881 aacccttctt tgtcggaatc taatataaca acaagagaaa cctctgggat atccaatcct
     2941 tcccttaata aattgatacc aattagaaca tcgaattcac cgagtcttag ctgacgaatt
     3001 aactccgtcc gttctaaacc ttcaatttct gagtgtaaat accgtaccct aatatttttt
     3061 tttgataaga attcagccaa atcctcagcc attcttttcg ttaatgttgt tactagtgtt
     3121 ctttggttct tgctaactct gttctgaatt tcaaaaatta attcatcaat ttgatttttt
     3181 gaggatctaa tttctacaat aggatcaact agtcctgttg gtctgactag ttgttctaca
     3241 acttggaagc tagaatttaa ttcataagga ccaggtgttg cggatacaaa aatcactttt
     3301 cttaagtatc tttcaaactc ctcgaatttt agtggacgat tatcaaatgc acttggaagc
     3361 cgaaatccat aagtcactaa catttctttt cttgtatgat ctccattata cattcctctt
     3421 aactgtggaa gtgtaacatg ggactcatct atgactagta aaaaatcgtc tccaaaaaag
     3481 tctaataaac agaatggcgg ttctccttct tttctgccat caaaatgacg agaataattt
     3541 tctatacccg aacaatagcc tagttcattt atcatctcaa tatcgtattt ggttcttgaa
     3601 gccaatcttt ccttttctat ttctccttct aatttaggaa gccatccttt tagttccttt
     3661 tttatcattc ttattgcatt ttctctactt tttttgtcgg taa
//
LOCUS       DATAFW010000121         6021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5182126, whole genome shotgun sequence.
ACCESSION   DATAFW010000121 DATAFW010000000
VERSION     DATAFW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5182126"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..776
                     /locus_tag="VHJ57_02905"
     CDS             216..776
                     /locus_tag="VHJ57_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX2487512.1"
                     /translation="MSYQRTLRIQKERIGVVIGKKGLIKDEIEKKCKIDIHVDSEYGD
                     VILKANSTDPLNILNINKGLEVILAISKGFSPERAFTLFMEDNTLEVVDLRDYVGKSA
                     NSLARVKSRLIGEKGKSRKTIEELTGSYISIYGHYVGIIGTSEQINLTTEAITRICSG
                     RSHKNVYNFLQEIRRRAKIEKLKLWE"
     gene            complement(1059..1619)
                     /locus_tag="VHJ57_02910"
     CDS             complement(1059..1619)
                     /locus_tag="VHJ57_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX2487513.1"
                     /translation="MGIIIAITGGSGVIYGIKLIESLNLLKMETHLVISEWGEKTIKI
                     ETNLDIEYIKKLSTNWYDNNNMAAPISSGSFKTDGMVIIPCSMKTLASIANGLDDSLI
                     SRAASVCIKENRKLVVVPRETPLSKIHLENMVKLSELGVSILPAMPGFYFHPSTIDEL
                     LFHIVGKVLDQFDIENNMFNRWGVKK"
     gene            1697..2593
                     /locus_tag="VHJ57_02915"
     CDS             1697..2593
                     /locus_tag="VHJ57_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX2487514.1"
                     /translation="MLTIFGTIALDTTKTSKKTVKRIMGGAATYASLSSSKYTPTNII
                     GIIGYDFPQEYRAILDRHVNTKGIIVKDNEKTFHYDSSFDSTLTVRTANKTELNVIEN
                     FEPFVPEEFIDSKYIFLANNDPVQNKKMQRLFSDPEFVVCDTIEYWINNNYDQVIDMI
                     KDVNGIIIDSQEARLLTHEYNLLKCAKKIKEFGPELIIIKKGENGSILFYEDIISVCP
                     AIPIENVVDPTGAGDCYAGGFLGYLSKKGKINTRTIREAAIYGNIMGSFAIEDFGPNQ
                     IINISCNDIEKRLKFYKEILMV"
     gene            complement(2635..3435)
                     /locus_tag="VHJ57_02920"
     CDS             complement(2635..3435)
                     /locus_tag="VHJ57_02920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX2487515.1"
                     /translation="MSSYQSRINNCSIKKKTPIILALDYDFDNYNNNYLYDKCKEIIT
                     IIESRICAIKINFHLLLSLSHTQIGELNIIAHKYDLQSIADIKLNDIGNTNKVTINKL
                     QKLGFDAVIVNPFIGVTEMESIVNYAHSIKFGIISLVYMSHPSSHEGYGLQISTSHNE
                     DPNNEYPNKKLIPMYKLLYNYSRMSDADGIVIGATKIDIIKEISELNELKIYSPGFFT
                     QNGSINDAVEAGTDYFIIGRHILNSSSPLDNLNLILDQVKKGKKFSQE"
     gene            complement(3441..4127)
                     /locus_tag="VHJ57_02925"
     CDS             complement(3441..4127)
                     /locus_tag="VHJ57_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487516.1"
                     /translation="MQSKSPFYVEINDLNNFARLVCALERAPLPTFSMYLKDEHVFAV
                     QTEFIYGIPVIYYVKFANSNLSNQYLAYRCHGLSETVTLVDIASNPLFVYSPIINVEK
                     LPQVMEKSSRKSRRSSYTKIMLRDLESLAKVAAYKTIYDEPPLPIFSFVNGKKKTFTL
                     GTSLNFMDTDSLTYFYYITIDKEPEKFLKYSSQKSAKPFFTNHIEEHGYIYIKIIKLS
                     KEHPLVINED"
     gene            4554..5009
                     /locus_tag="VHJ57_02930"
     CDS             4554..5009
                     /locus_tag="VHJ57_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487517.1"
                     /translation="MIRLISWCTGLLNPDAGIIKKTVNYCLFILIYKEIIVDLKELDI
                     DKKILSSINLLTNAINNKDTEKSEIWKAYCWLEYTILLVRLKKYNLLDEIQRNELKNI
                     KKEEKVEPEILLRKARDILASLNYMNDDQLLNSLRVSRDFLRIFLKTKK"
     gene            complement(5029..>6021)
                     /locus_tag="VHJ57_02935"
     CDS             complement(5029..>6021)
                     /locus_tag="VHJ57_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HEX2487518.1"
                     /translation="IVVGGFFGDEGKGKIIAYIVKKDNINLAARGGVGPNAGHTFNLN
                     GQTFKVRMLPSAAFNVKTSLAIGAGVLVNPKILLNEISSFNAQGRTFVDRNCGIIKEE
                     HVMQDSTEGFLKDKIGTTGTGTGPANSDRALRKLSLAKDCLEIKDYLGEVSSMIHSNL
                     DKKNSVLLEGTQGTYLSLYHGGYPFVTSKDVTASSVCADVGIGPKSINEVLVVFKSYI
                     TRVGGGPLENELERVESQKRGWLEYGSVTGRERRSSPFNIELAKRSIRLNGATSFALT
                     KLDVLYPECAGVKDYSKLTPSAKKFVEEIEAELGLKAELIGTGADVDSVIDQRS"
BASE COUNT         2128 a          929 c          905 g         2059 t
ORIGIN      
        1 cctattaaaa agatgttcga tagctaattt aattggaaga agagttgtag aaaaaacctt
       61 gagtttgggt cttgcaaaca gaaatagtat aaaaattgtt tcagatattc cttttctaat
      121 gatatttaaa ttttataaca agtattaggt tttaattttt aataatttat attttatatg
      181 caacaaatat tattactagg gaaggcttat tcaaattgag ttatcaacgt acactaagaa
      241 tacaaaaaga acgaattgga gtagttattg gaaaaaaggg attaataaaa gatgagatcg
      301 aaaaaaaatg caagatcgat attcatgttg atagtgaata tggagatgta attctaaagg
      361 ctaacagcac agatcctttg aatatattga atataaataa aggattagaa gtaattcttg
      421 caatatctaa aggtttttct ccagaaagag catttactct atttatggaa gataacactc
      481 tagaagtagt ggatttaaga gactatgttg gaaaatcggc aaattcatta gctagagtaa
      541 aaagtcgact aataggagaa aaaggaaaat ctaggaaaac tatagaggaa ttgactgggt
      601 cttatatttc aatatatggc cattacgtag gaataattgg cacgtctgaa caaatcaatt
      661 taacaactga agcaataaca cggatttgtt caggtcgatc ccacaaaaat gtatataatt
      721 tccttcaaga aataagaagg agagcaaaaa tcgaaaaact caaattgtgg gagtaagatt
      781 gcagcatcta aaatttaaaa aatataaata catcaaggaa aactaaacga tattgaagcc
      841 ccaagatgaa aataaaaaag tttcaacgag atcgtactac aagatgagta ctctcctggc
      901 aattattata actatgccag gtattgcagc tctattcaca ttaagacact ttaacattga
      961 cattatttat caagtaatca ttagctcttt aatattttta atatgtatag gtttctcctt
     1021 taagatttct aaatatcttg tcaaattagg ataaaaaatt atttttttac tccccagcga
     1081 ttaaacatat tattctctat atcaaattgg tccaatactt ttcctacaat atgaaataac
     1141 aattcatcaa tagtagatgg atgaaaataa aagccaggca tagctggtaa tatggatacc
     1201 cctagttcgg acaatttaac catattttct aaatgaattt tagacaaagg ggtttctctc
     1261 gggacaacta ctaattttct attttctttt atacagacac tggcagctcg tgaaatcaaa
     1321 ctatcatcta atccattggc tatactagct agtgttttca tactacatgg aataatgacc
     1381 atcccatcag tcttaaatga accactagag ataggtgctg ccatattgtt attatcgtac
     1441 cagttggttg ataacttttt tatatattca atatctaaat ttgtttcaat tttaatagtc
     1501 ttttctcccc attcactaat aactaaatgt gtttccattt taagcaaatt taatgattca
     1561 attaatttta taccataaat aactcctgat cccccagtaa tggcaatgat aatacccata
     1621 ttttcactta attaaataga tgataagtat ttaactaaaa attaattttt ataatcctta
     1681 attcaccaaa taaacgatgc ttactatttt tggaacaata gccttagata caactaaaac
     1741 ttcaaagaag accgtaaaaa ggataatggg cggagctgca acatatgcat ctctatcttc
     1801 ctcaaaatat acaccaacta atattattgg gataattggt tatgatttcc ctcaagagta
     1861 tagagcaatt ttggacaggc atgtgaatac taaaggtatc attgtcaaag acaatgaaaa
     1921 gacctttcat tatgattcct cgtttgattc aactttgaca gttcgaactg ccaataaaac
     1981 tgaattaaat gttattgaaa attttgaacc tttcgtgcct gaagaattta ttgattctaa
     2041 atatattttt ttagcaaaca acgatccagt tcaaaacaaa aaaatgcaac gtttatttag
     2101 tgatcctgaa tttgtggttt gtgatactat agaatattgg attaacaata attatgatca
     2161 ggtcatagat atgataaaag atgtaaatgg aataataatc gatagccaag aagcacgact
     2221 tttaacacat gaatataacc tcctaaagtg tgctaaaaaa attaaagaat tcggacctga
     2281 gttgataatc ataaaaaaag gtgaaaatgg atctattctt ttttatgagg acattatatc
     2341 tgtatgtcca gctatcccta tagaaaatgt ggttgatcca actggagcag gagattgcta
     2401 tgcaggaggg tttcttggtt atttgtcaaa aaaaggaaag ataaatacaa gaactattcg
     2461 agaagctgca atatacggaa atatcatggg ttcgtttgca atagaagact ttggaccaaa
     2521 tcaaataatt aatatttctt gtaatgacat agagaaaaga ttaaaattct ataaagaaat
     2581 attaatggta taatccgggt tgtaaataag ttgtgaataa cacattctat aaaattattc
     2641 ttgactaaat tttttacctt tctttacctg atctaatatg agatttagat tatcaagagg
     2701 tgatgatgaa tttaatatgt gtctacctat tatgaaataa tctgtaccag cttctactgc
     2761 atcatttatt gaaccatttt gggtaaaaaa acctggcgaa taaatcttta actcatttaa
     2821 ttcagaaatt tccttgataa tatcaatttt ggtggcacct ataactattc catctgcgtc
     2881 actcatacga gagtaattat aaagtaattt gtacatggga attaattttt tattaggata
     2941 ttcattattt ggatcctcat tatgagaagt agaaatttgt aatccgtagc cttcgtgaga
     3001 actgggatga ctcatatata caagagagat aataccaaat tttatggaat gggcataatt
     3061 tacgatggac tccatttctg ttactccaat aaatggattt acgatcactg catcaaatcc
     3121 taatttttgt aacttattga tagtcacttt atttgtattt cctatatcat tgagctttat
     3181 atctgctata ctctgaagat catatttatg agcaatgatg ttaagttctc ctatctgagt
     3241 atgcgataga gacaataaaa gatggaagtt tatttttatt gcacaaattc tagattcaat
     3301 aattgttatt atttctttac atttatcata caagtaatta ttattgtaat tatcaaaatc
     3361 ataatccaat gctaagatta ttggggtttt ttttttaatt gagcaattat ttatccttga
     3421 ctgataagaa gacataattt ttaatcctca tttatcacga ggggatgttc tttagacaat
     3481 ttaatgattt ttatataaat atacccatgt tcttctatat ggttagtaaa aaaaggtttt
     3541 gctgattttt ggctagagta ttttagaaat ttttctggtt ccttatcaat tgtaatgtaa
     3601 taaaaataag ttaaggaatc ggtatccatg aaatttaaag aagttcctag cgtaaatgtt
     3661 ttttttttcc cgtttacgaa agaaaatatt ggtaatggag gttcatcata aattgttttg
     3721 tatgctgcga ctttagccag gctttctagg tctcttaaca taatctttgt ataactagat
     3781 ctcctacttt ttctcgaact tttttccatt acctggggta gtttttcaac atttataatt
     3841 ggagaataaa caaaaagagg attagaagca atatctacca atgtcacagt ttcagaaaga
     3901 ccatggcatc tatatgcaag atattgatta ctgagatttg agttcgcaaa tttaacataa
     3961 taaatcacag gaataccata aataaattca gtttgtacag caaagacatg ttcatctttt
     4021 aaatacattg aaaaagttgg taatggagca cgttctaatg cgcaaactag tctggcaaaa
     4081 ttattcagat catttatttc aacatagaat ggtgattttg attgcaagca attatcttca
     4141 aaaaagagcc tattaatatt ttacactttc tacttgatca tcaggcgata tcatatcgat
     4201 agatttaaag agatcataat cgctttatct cgaccatact agtcaatata ctttaatcct
     4261 taatactaat ctgaaaaatt ctaaaactag tgaaatggat agtattccat acttttatcc
     4321 ttcttagttc aaaataatca caacaaattt ttcgttttaa agtttagaaa atatcacatt
     4381 aggagactca aattaggttt acgatctata atattctttg ttaacaccat gcaaaattac
     4441 cttaatcaat tcaaattcta ttgattcctg caaaattgtc aacagtttct gccgctaatc
     4501 gagtaaattt ttgatatcta ttactttttg tctttgctta aaagcattac attatgataa
     4561 gattaatttc ttggtgtact ggcttactaa atccagatgc tggtataata aaaaaaacag
     4621 tcaattattg tttatttatt ctaatataca aggagataat agtggattta aaagaattag
     4681 acatagataa gaagattctc tccagcatta atttgttaac taacgctata aataataaag
     4741 atacagagaa gtctgaaatt tggaaagctt attgttggtt agaatatacc atacttttgg
     4801 tccgtctaaa aaaatataat ctattagatg aaatacaacg aaatgaatta aaaaatatta
     4861 aaaaggagga aaaagtagaa ccagaaatcc tcttaagaaa ggcaagagat atactcgcga
     4921 gtttgaatta tatgaatgat gatcaacttt taaattcttt gagggtttca agagattttt
     4981 tgagaatttt tttaaaaaca aaaaagtaat acaatcatat tgaaaacttt aggatctttg
     5041 atcgattacc gaatctacgt ctgctcctgt gcctattaat tctgccttaa gtccaagttc
     5101 tgcttcaatt tcctcgacaa acttttttgc cgatggagta agtttagaat aatctttaac
     5161 accagcacat tcaggataaa gaacatctaa ttttgtaagt gcaaacgatg ttgccccatt
     5221 tagtcttatt gatctttttg ctaattcaat attgaaagga gaagaccttc tctcccttcc
     5281 agttacgctt ccatattcaa gccatcctct cttttgggat tctactcttt ctaattcatt
     5341 ttctaatggc ccacccccga ctcttgtgat ataagattta aatacaacaa gtacttcatt
     5401 aatggatttg ggtcctattc ctacatctgc acagacagaa gaagcagtaa catcttttga
     5461 cgttacaaaa ggatatcccc catgataaag agaaagataa gttccttgtg taccttccag
     5521 cagaacgctg ttctttttat ccaagtttga atgaatcata gaacttacct cacccaagta
     5581 atcctttatc tctaaacaat ccttggcaag agaaagtttt cttaatgctc ggtcagaatt
     5641 ggcgggtcca gttcctgttc ctgtagtacc tattttgtct ttaaggaatc cttctgtgct
     5701 atcctgcata acatgttctt ctttgattat accacaatta cgatcaacaa aggttctacc
     5761 ctgagcatta aaggaggata tctcatttaa tagtattttt ggatttacca aaactccagc
     5821 accaatagct aaactagtct taacgttaaa ggcggcactg ggaagcatac gcaccttgaa
     5881 ggtttgtcca tttaaattaa aagtatgtcc agcattagga cctactcctc ccctcgctgc
     5941 taaattaata ttatcctttt taacaatata agctattatc tttccttttc cttcatctcc
     6001 aaagaagcca cctactacta t
//
LOCUS       DATAFW010000122         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6240212, whole genome shotgun sequence.
ACCESSION   DATAFW010000122 DATAFW010000000
VERSION     DATAFW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6240212"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="larC"
                     /locus_tag="VHJ57_02940"
     CDS             <1..484
                     /gene="larC"
                     /locus_tag="VHJ57_02940"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HEX2487519.1"
                     /translation="DVGNEKMFLIETNIDDVNGEIVGNLIEVLMEEGARDVTIIPGIT
                     KKNRPVNIIRVISDIVKTNSLIDKLFKETGTSGIRINEINRITLERNIIIIKVTINHS
                     KYDVKVKITKDSSNNIINIKPEFNDLKKIANSEDSSLKAIQELVMFQIFKKFKANSYE
                     "
     gene            477..1292
                     /gene="larE"
                     /locus_tag="VHJ57_02945"
     CDS             477..1292
                     /gene="larE"
                     /locus_tag="VHJ57_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX2487520.1"
                     /translation="MSSEQSLLQLKEWFKKNTKGNVFIALSGGVDSAIVALAAKKALG
                     EKAIAITANYKTLALFELEDAKRVAHEIGIQHIIIEYNELEDENFVKNDINRCYYCRK
                     SLGENIKKYEKEYHVTDIVDGTHLDDTNDYRPGLKAMREYKIKSPLLEQRIGKKEIRE
                     IAREYNISVANKPSNSCLASRIPYGNNITQEKLKKIEIAEEKVKELFDISHVRVRDHG
                     EIARIEVGRDEMEKMFNKIKLNQLDLELKNIGFKYASLDLYGYRSGQLIVIND"
     gene            1285..>2036
                     /locus_tag="VHJ57_02950"
     CDS             1285..>2036
                     /locus_tag="VHJ57_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX2487521.1"
                     /translation="MTNLSENKSNNHLIYLDHAASTPVLQETINEMIPYLGDLYGNPS
                     SIHTYGIKSKMAIRIARRRVASLIGAKSSEIFFTSGGTESDNFALKGISKCIRNYQGE
                     KNHIITSSIEHDAILETCHYLEKDGFTVAYLNVDKKGIIDRTELKNHLTEKTALVSIM
                     LANNEIGVIQPIKEFSEIVHKFDKRIIFHSDAVQAVGKIPVNVKELDLDSLSLSSHKI
                     NGPKGVGALYVREKVNFEPLIQGGGQELTFRSG"
BASE COUNT          832 a          238 c          381 g          585 t
ORIGIN      
        1 tgatgtaggt aatgaaaaaa tgtttttaat tgaaacaaat atagatgacg taaatggaga
       61 gattgttggt aatttaatag aagttttgat ggaagaagga gcaagagatg taacaattat
      121 tccaggaata accaagaaaa atcgacctgt aaacataata agagttattt ctgatatagt
      181 taaaactaat agtcttattg ataagttatt taaagaaact ggaacatcag gtataagaat
      241 aaatgaaata aatagaataa ctttagaaag gaacataata attataaagg taactattaa
      301 tcattcaaag tatgatgtca aagtaaaaat aacaaaagat tcatcaaata acataatcaa
      361 tatcaaacca gaatttaatg atttgaagaa aatagcaaat agtgaagata gttccttaaa
      421 agcaattcaa gaattagtta tgtttcagat atttaaaaaa tttaaggcga atagctatga
      481 gtagtgaaca aagtctttta caattaaaag agtggtttaa aaagaataca aaaggaaatg
      541 tattcatcgc attatcaggt ggggtagata gtgcgatagt ggctttagct gcaaaaaaag
      601 ccttaggtga gaaagcaatt gctattactg ccaactacaa aactctggca ttatttgaac
      661 tagaagatgc aaagagagtg gcccatgaaa taggaataca acatataata attgagtata
      721 acgagttaga agatgagaat tttgtaaaaa atgatattaa caggtgttac tactgtagaa
      781 aaagtttggg ggaaaatatc aaaaaatacg aaaaggaata tcatgtaact gacatcgttg
      841 atggtactca tttggacgat actaatgatt atcgtcctgg cttgaaagcg atgagagaat
      901 ataaaatcaa aagcccgtta ttagaacaaa gaattggaaa aaaagaaata agagaaattg
      961 ctagagaata taatattagt gttgcaaata agccttctaa ttcatgttta gcttctagaa
     1021 ttccttatgg taataacata actcaagaaa aattaaaaaa aatagaaata gcagaagaaa
     1081 aggttaagga gttatttgat ataagtcatg ttagagtaag ggatcatggg gagatcgcaa
     1141 gaatagaagt tggaagagat gaaatggaaa aaatgtttaa taaaataaaa cttaatcagt
     1201 tagatctaga attaaaaaat attggtttca aatatgcgtc tttagattta tacgggtata
     1261 gatctggtca gctaatagtg attaatgact aacttgagtg aaaataaatc caataaccat
     1321 ttaatttacc tggatcatgc tgcatcaact cctgtcctcc aggaaacaat taatgaaatg
     1381 ataccatatc taggagattt atatggtaac ccttcttcaa tccataccta tggaataaaa
     1441 tcaaagatgg caattcgaat tgccagaagg agagttgcct ctcttatagg tgctaaatcc
     1501 agtgaaattt tttttacatc gggtggaact gagtcagata attttgcttt gaagggaata
     1561 agcaaatgta ttagaaatta tcaaggggaa aaaaaccata ttattactag ttctattgaa
     1621 cacgatgcaa tactagaaac ctgtcattat ttagaaaagg atggctttac cgtcgcctat
     1681 ttaaatgtag ataaaaaagg tattatcgat agaactgaac taaaaaacca tcttacggaa
     1741 aaaacagcgt tagtcagtat aatgctagct aataatgaaa tcggtgttat tcaaccaatc
     1801 aaagaattct cagaaattgt tcataaattt gataaaagga taatatttca ttctgatgct
     1861 gttcaagcgg taggaaaaat accggtaaat gttaaagaat tggatcttga ttcattatca
     1921 ttgtcttctc ataaaattaa tgggccaaaa ggagtaggtg ccttatacgt gcgtgaaaag
     1981 gtaaattttg aacctttaat tcaaggaggc ggacaagagt taacctttag atcagg
//
LOCUS       DATAFW010000123         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_73705, whole genome shotgun sequence.
ACCESSION   DATAFW010000123 DATAFW010000000
VERSION     DATAFW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73705"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1998
                     /gene="polX"
                     /locus_tag="VHJ57_02955"
     CDS             58..1998
                     /gene="polX"
                     /locus_tag="VHJ57_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX2487522.1"
                     /translation="MIDYLVKTKTFFSDAITITIQLRVQCFVRSLGLSLVMLHRENVA
                     IMSKNSEVAKIFREIAFILQTAEEKKSEPNTIFKIRSYNRAAEEIQNLSSDIGDIYEK
                     EQLKGLLKIPSIGKAIATKLEEYITTGKIHYYDELKEKLPVDVSQFFGLEGIGPKTIK
                     MLYDNLGIKNISDLEKAALEGKIKTVPGFSQKKEELILKKIQFFRKDGGRRLIGEIYP
                     LVRRIEKRLSANTGVKKAVAVGSFRRMKETIGDIDFLVSVMSEEDGDNIIDYFVNMPE
                     VNEISGRGLSKAFVKLNNGIDADLLVVPEESFGAAMQYFTGSKEHGVAMRKIAISKGL
                     RLNEWGVFDKQNCRVAGATEEGVYQILGLEWIPPEMRENRGEIELAKMKKNGMEKEER
                     SRLLQLIAYDDLKGDLQVHSNNTDGTMSIEEMALNARDKFELSYIAISDHTKSLRLAN
                     GLDEKQLLNQANTIAELNDKIKMNNNNKNKHFRILSSAEVNILKDGSLDISNNVLDKL
                     DIVGAAIHSHFSLPIELQTARLINAAQNPSIDIIFHPTGRIINRREGYPVNISKLIEV
                     ANESNTILEIDAYYDRLDLKDEYIRMAVENNVKLVIDSDAHHPLHYAFLLFGIGQARR
                     GWAKKSDILNTLSAIELLDNLK"
     gene            2135..2392
                     /locus_tag="VHJ57_02960"
     CDS             2135..2392
                     /locus_tag="VHJ57_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487523.1"
                     /translation="MVRNYSKDIYPEDIVWDDIIKKEAKGIDNITLGEVQEIAPEYIV
                     TEKGTLDNDKFLFPKDLVERFDGHIIWIRVTKEEANRYRRA"
     gene            2993..3115
                     /locus_tag="VHJ57_02965"
     CDS             2993..3115
                     /locus_tag="VHJ57_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487524.1"
                     /translation="MRLVDYLQGRKDKKTCRICNYIAKNEIELERHMKKEHETY"
BASE COUNT         1297 a          384 c          598 g          984 t
ORIGIN      
        1 tttattttta tatttaaaat aaaaatagtg aagacaaatg ctggataata acatgaaatg
       61 attgattacc ttgtaaaaac gaaaaccttt ttttctgatg ctattacaat tacgattcaa
      121 ttacgcgtac aatgtttcgt cagatcgctt ggtttaagtc tagtaatgct acatagagaa
      181 aatgtagcaa taatgagcaa gaattctgaa gttgccaaaa tatttcgaga aatagcattt
      241 attttgcaga cagcggaaga gaagaaaagc gaacctaata caatttttaa aattagatca
      301 tataatagag ctgcagaaga aatacaaaac ttatcttcgg atataggcga catatacgaa
      361 aaagaacaac taaaaggatt gctaaaaatc ccctcaattg gaaaggctat tgctacaaaa
      421 cttgaagagt atattacaac aggaaagata cattattacg atgaactaaa agagaagttg
      481 cccgtagatg tcagtcaatt tttcggtttg gaaggtatag gcccaaaaac aataaagatg
      541 ctgtatgata atctaggaat caaaaatatc tctgatttag aaaaagctgc gttagaaggt
      601 aagattaaaa ctgttcctgg attttctcaa aagaaggaag aattaattct aaagaaaatt
      661 caatttttta gaaaagatgg aggacgacga ctaattggtg agatctatcc tttggtaagg
      721 cgaatagaga aaaggctttc agcaaatact ggagtaaaaa aagcagtagc tgtcggttca
      781 tttagacgaa tgaaggaaac tataggagat attgattttt tggtgtcagt tatgtccgaa
      841 gaagatggtg ataatataat tgattatttt gtaaatatgc cagaagttaa tgagataagc
      901 ggaagaggat tatctaaagc atttgtcaaa ctaaataacg ggattgatgc agatttactt
      961 gttgtacctg aagagagttt tggtgcagcg atgcaatact ttaccgggag taaagaacat
     1021 ggagtagcta tgagaaaaat agccatatct aaaggtcttc gtctcaatga gtggggggta
     1081 tttgacaaac agaactgtag agtagctggc gctactgaag aaggagtata tcaaatattg
     1141 ggcctagaat ggattcctcc agagatgcga gaaaatagag gagaaataga gctagcaaaa
     1201 atgaagaaga atggaatgga aaaagaagag agatcaaggc ttctacaatt aatagcatat
     1261 gatgatttga aaggagactt acaagttcat agcaacaaca cggatggcac aatgtctata
     1321 gaagaaatgg cattaaacgc aagagacaaa ttcgaattga gttacattgc gatctcagat
     1381 catacaaaaa gtttgagact tgctaatgga ctggatgaaa aacaattatt aaatcaagca
     1441 aatacaattg cagagctgaa tgacaaaatt aagatgaata acaacaacaa gaacaagcat
     1501 tttcgtatat tatcttctgc agaagtaaat attctaaagg atggatcact tgatatttca
     1561 aataatgttt tggacaaact ggacatagta ggtgctgcta ttcattctca tttttcttta
     1621 ccaatagagt tacagacggc tagactcata aatgcagctc aaaatcctag tattgatatt
     1681 atatttcatc caacaggcag aattattaat cgaagagaag gttatcctgt caatatatcc
     1741 aagttgattg aagtggcgaa tgaatccaat acgatccttg aaattgatgc atattatgac
     1801 cggttagatt taaaagatga atacataaga atggctgtcg aaaataatgt taaattggta
     1861 attgattctg atgctcatca tcctttacac tatgctttcc tactatttgg tataggacaa
     1921 gctaggagag gatgggctaa aaagtctgat atacttaata cactatcagc tatagaatta
     1981 cttgataatc tcaagtagtc aaatcgaagt attctttctt aaccagttag tgaacaaaac
     2041 agacacagat cctaacaatt acattttacg gttttttata ttatttaatt ttcgtcgaac
     2101 caaatattat ataaaataaa taactattaa aattatggta agaaattata gtaaagatat
     2161 atatccagaa gatatagttt gggatgacat tatcaaaaag gaagctaagg ggatagataa
     2221 tataactcta ggagaggttc aggaaattgc tcctgaatat atagtaacag agaaaggcac
     2281 gttggataac gataaattcc tttttccaaa ggatctggta gagcgttttg atggacatat
     2341 tatttggatt agagtaacaa aagaagaagc aaataggtat agaagagcat agtaaaactt
     2401 aatgttattt aaataatttt tgtctatttg gcaaaaaatt tgcaattata atatacaata
     2461 tccaaactaa tatagttgtg ataagggaaa ttagagaggg atcacttaaa atgaacttaa
     2521 aaattataaa agttgaaaga aatttagtaa taggaaattt ctgcaaaact aattatagtt
     2581 attagttttt taaatcttgt tttattttta gatctttgtt tccattagaa ataacatatt
     2641 gagctttatt atctaagaaa tagagcaaaa taaacaaaat attataaatt gaaaaatcta
     2701 ccattgtgga aatagatgaa attggtaaat aatttgtaga atataatagg aatgaataat
     2761 ttaaaatccg tgtatctgat attgctgtta ttattgcaga agtaaaatta tatttttggt
     2821 ttatattttt aatttaatat attcaaatca taagtttatt tactattatg cattttcata
     2881 atgtgtatat attatttggc aaataaaaga ttgaaaaaaa gaaaaattat tgtaaaagtt
     2941 ataaataaaa tcttgtattt aataatttat gtggggtata acaatatatc taatgcgttt
     3001 ggttgattat ttacagggac gtaaggataa aaaaacgtgt aggatatgta attatatcgc
     3061 taaaaatgaa atagaacttg aaagacatat gaaaaaggaa catgagacct attaattaaa
     3121 ctacatttaa ttagattatg cgaacgtgat ttaaggtaat aataacttct aaataatgca
     3181 tacaagaaag aaaaagacag taatttaaag gaaagaatac tacttgtaaa acgtgtcaag
     3241 atagacaaac aacaagcggc atc
//
LOCUS       DATAFW010000124         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4302137, whole genome shotgun sequence.
ACCESSION   DATAFW010000124 DATAFW010000000
VERSION     DATAFW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4302137"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VHJ57_02970"
     CDS             <1..588
                     /locus_tag="VHJ57_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487525.1"
                     /translation="VSSEVRINDSPRHFMVLDAIARGMQDPNKIAKVLKINKEEVELI
                     VNDLLTQRLIIKKENRGFFGNKKIKISITDTGIRLLNSKKEELERKWHQAQKMYNNGK
                     GNRAQLQTYMENNRSWVPLMIFSGIIDVLFFMTMMSFLGMALNPMEQSMAGESGAEGA
                     AAGGESSTEETSGSGADTGDTGDFGGFDAGGFGDF"
     gene            complement(699..1202)
                     /locus_tag="VHJ57_02975"
     CDS             complement(699..1202)
                     /locus_tag="VHJ57_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487526.1"
                     /translation="MSSKKKIKIELQDDEGSKYNLSLEGNFSKEKIFQVIELMDLVRS
                     TNSGEITNQLQEKNNNNTLSVDSKIWKIVEESFYFNTFTSSDIVNIYKKKYNETIKLS
                     IISTYLARYHAKGKLNRIKKQREYVYNLVRNPSIKADSINDTVSSDDDNKIAYDVSRK
                     TIDDLKN"
     gene            complement(1339..1641)
                     /locus_tag="VHJ57_02980"
     CDS             complement(1339..1641)
                     /locus_tag="VHJ57_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HEX2487527.1"
                     /translation="MIMEFSFFYSSSLVAFIIGIAATVLTRYSSIPQIIKGFKTKKMD
                     DVSFWLIFYLTVGLFLYVIYGIIIDDAIIIWGNAIGVISNLILVILKIKYSVKSFE"
BASE COUNT          663 a          223 c          267 g          645 t
ORIGIN      
        1 gtgtcttctg aagtaagaat taatgatagt ccacgtcatt ttatggtact tgatgccatc
       61 gctagaggaa tgcaagatcc aaataaaatt gcaaaagttc tcaaaattaa taaggaagaa
      121 gtagaattaa tagtaaatga tcttttaact caaagactaa tcataaaaaa ggagaatagg
      181 ggtttttttg gtaacaaaaa aattaagatt tctattacag atacaggaat tagactcctg
      241 aattctaaaa aagaagaatt agaacgcaag tggcatcaag ctcaaaagat gtataacaat
      301 gggaaaggaa atagagctca attgcaaaca tatatggaaa ataatagatc atgggtacct
      361 ttaatgatat tttcaggaat aatagatgtt ttattcttta tgactatgat gtcttttttg
      421 ggtatggcat taaatccgat ggaacaatcg atggcaggtg aaagtggagc agaaggtgct
      481 gctgcaggag gtgaatcaag taccgaagag acgagcggta gcggagcaga tactggagat
      541 acaggagatt ttggaggatt tgatgcagga ggatttggag atttttgatt tccaaaattt
      601 attttgtttt acaggacgta cgtcaatagc taatttaatt aatagcaaat ctatagttat
      661 agaaattagt taatttattt tataaatata tttataaatt aatttttaag atcatctatt
      721 gtctttctac taacatcata ggcaatttta ttatcatcat cagatgaaac tgtatcatta
      781 atagaatctg ccttaattga aggattccta actaaattat acacatattc tctttgtttt
      841 ttaattctat ttaatttacc ttttgcatga tatcttgcaa gataagttga tataatactt
      901 aatttgattg tctcattata ttttttttta taaatattaa caatatcaga agaggtaaat
      961 gtattaaaat aaaaagattc ctcgactatt ttccaaatct ttgaatcaac agataaagta
     1021 ttattattat ttttttcttg taattgatta gttatttctc cagaatttgt tgatcgtact
     1081 agatccatga gctcaataac ttgaaatatt ttttcctttg aaaaattacc ttctaatgag
     1141 agattatact tactcccttc atcatcttgc aattcaatct ttattttctt cttcgagctc
     1201 aaagtaaaag atctttgttt tgtaagccat ttaatgcttt taaatataat agtaaataaa
     1261 aattaagtaa ttgctaaact ttgttaatag aatgtttcta ctaactaaaa ggactaaagt
     1321 aaacatattt aagaatcatt attcaaatga tttgacagag tatttaattt tgagaataac
     1381 tagtattaga tttgaaatta cacctattgc gtttccccaa ataattatag catcatcaat
     1441 aattattccg taaattacat aaagaaataa tcctactgtt agataaaata ttagccaaaa
     1501 tgatacatca tccatttttt tagttttaaa accttttatt atctggggaa tgctactata
     1561 gcgggttaaa actgtagctg caatacctat aatgaaagct accaaacttg acgaataaaa
     1621 aaatgaaaat tccattatca ttgataatct ttaatagaag gttattttat tggtattgta
     1681 aatttaatta acagacttaa atcaaaaaag attttgttta aatttaaaag taggatttat
     1741 cttgtataaa ttagacatac ttataatatc tccttatcag acatttgtat agatccag
//
LOCUS       DATAFW010000125         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_779628, whole genome shotgun sequence.
ACCESSION   DATAFW010000125 DATAFW010000000
VERSION     DATAFW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779628"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VHJ57_02985"
     CDS             complement(<1..301)
                     /locus_tag="VHJ57_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487528.1"
                     /translation="MEFDVKIGATKERVVTTNSNQTTSFLWKGENVLSTPSMISEMEE
                     TCRLLLKDFVLKEKEWDSVGTIVDIKHIAITPVGSKIRLKAIIESVNNRRVLFNVE"
     gene            complement(426..1880)
                     /locus_tag="VHJ57_02990"
     CDS             complement(426..1880)
                     /locus_tag="VHJ57_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEX2487529.1"
                     /translation="MAFDSLSEYIDTLESVGKLHRVKTEVDTNLEIAEIMRRMMYSGD
                     SPAILFENVKGYDIPVLGNAFGTMDLLKIALNLSDFREIGKRITDLTKLKIPSNLFDK
                     LKMLPRLSELGDYAPKNVESGAVTEIIETEKANLNSLPILKSFPNDAGKFITFGLTIT
                     KHPETGIRNIGVYRIQIVNDQKAIMHWQTHKRGAQHYEIMKEYNKPIETAIVIGTDPS
                     TIFSGIAPVPEGMDKFFFSGIVRKRGVKLVKCKTVDLEIPVNSEIVLEGWVDPNEMRI
                     EGPFGDHTGFYTPPEPYPTFNLTCLMRRKNPIYLTTVVGKPILEDAYIGKVIEQSFLP
                     LIQMFQPEVIDFSMPASGWFQGLAIISIKKRYPGQAKKVMMGLWGMGQLSLTKIFVVV
                     DHDINVHDINEVIWAITTKSDPKRDIIIIDNTPTDTLDPASPILNLGSKMGIDATTKT
                     AEEGFLREIQKPVEVDYDTKNLVDKKWINYGFSN"
     gene            complement(2236..2808)
                     /locus_tag="VHJ57_02995"
     CDS             complement(2236..2808)
                     /locus_tag="VHJ57_02995"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX2487530.1"
                     /translation="MDIDKNQFKEKIIHRLSSKINVDVQLLKSSGYTLSSVLVIIHFT
                     DFNPKIILTKRSSLLKYHKNEISFPGGTFKETDSSLLNTALRETKEEIGLNFHSNEIV
                     GSFHTVRTLTSNFAIIPFVTFQDSIRKTRILIDEVSEILDIPVFDLFKGFNNNFNYKK
                     FSDSYTFRYNSEIIWGATARILKQIRDCFY"
     gene            3021..>3421
                     /locus_tag="VHJ57_03000"
     CDS             3021..>3421
                     /locus_tag="VHJ57_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487531.1"
                     /translation="MIINYELNPPKILPNDYFSYSNLLSDIENVKNKAMILDEYINCL
                     HFTDSVLGVPRLSSITMARIIKENNTKMKISCSVRTRDRNLTSMYQTISDSILLSIES
                     LLILLGDQSKNDLQIHGLLKPSKVVNQFNAQ"
BASE COUNT         1204 a          521 c          467 g         1229 t
ORIGIN      
        1 cctctacgtt aaacagtact cttctattat taacagattc tattattgct ttcaacctaa
       61 tttttgaacc tacaggagtt atagcaatat gtttaatatc aacaatggtt ccaactgaat
      121 cccattcctt ctcttttaaa acaaaatcct ttaaaagcag tctacaggtt tcttccattt
      181 cagaaatcat tgaaggagta gaaagcacat tttctccttt ccataaaaaa ctggtagtct
      241 ggtttgaatt agttgttaca actctctctt ttgtagcacc aatttttaca tcaaattcca
      301 attaatatca aaacataaaa catgattgaa tttattaaac taattgatag aataatgata
      361 atttggaaac taatatagaa aattgtaaag aaatcaaaat gaaaaacgaa tgatagtaga
      421 attgttcaat tagagaatcc ataattgatc cattttttat caactaaatt cttggtatca
      481 tagtcaactt ctactggttt ttgaatttct cgcaaaaatc cttcctcggc agttttagta
      541 gttgcgtcta ttcccatttt tgaaccaaga tttaaaattg gagatgcagg atcaagggta
      601 tcagtaggag tattatcaat tataattata tctcttttag gatcagattt tgtagtaatt
      661 gcccagatta cttcattaat atcatgtaca ttaatatcat gatcaacaac aacaaaaatt
      721 tttgttaatg ataattgacc catcccccat aatcccatca ttactttttt cgcctgtcca
      781 ggatatcgtt ttttgataga tattatagcc agtccttgga accaacctga ggcaggcata
      841 gaaaaatcaa tgacctcagg ttgaaacatt tgaataagag gaagaaatga ttgttcaata
      901 acttttccga tgtatgcatc ttccaaaatt ggtttcccga caacggttgt cagataaatt
      961 ggattttttc ttctcatcag acatgtcaaa ttgaatgttg gatatggttc aggaggtgta
     1021 taaaatccag tgtgatcccc aaatggtcct tctattctca tttcattagg gtcaacccat
     1081 ccttctaaaa caatctctga attaacagga atttctaaat ccactgtttt gcattttact
     1141 aattttactc ccctttttct aacgattcca gaaaagaaaa atttatccat tccttcagga
     1201 acaggagcta ttccactaaa aatagttgag ggatccgttc cgattactat tgctgtttca
     1261 ataggtttgt tatactcctt cattatttca tagtgttgag ctcctcgttt atgtgtctgc
     1321 caatgcatta tagctttttg gtcatttacg atttgaattc tataaacacc gatatttcta
     1381 attccagttt ctggatgttt ggtaatagtt aatccaaagg taataaattt ccctgcatca
     1441 ttgggaaacg attttagaat tggtagtgag ttaagattag ccttttcggt ttcaattatt
     1501 tctgttacag caccagattc aacattcttt ggagcatagt ctcctaattc agataatcta
     1561 ggaagcatct ttaatttatc aaacaagtta gaaggtattt tcaattttgt taaatctgta
     1621 attcttttac caatttctct aaaatctgag agattcagag caatttttaa taaatccata
     1681 gttccaaatg cattacctaa tacaggaata tcatatcctt ttacattttc aaaaagtata
     1741 gcaggggagt caccagaata catcatacgc ctcataatct ccgctatctc aagatttgta
     1801 tcgacttctg ttttcacacg atgtaatttg ccaacagatt caagtgtatc aatatattca
     1861 gatagactat caaaagccat aaatcaacaa ataactagtt tctagagtta taaaaacagc
     1921 tcttttactt taaaaaaagg aaatagtgga tttgaacgtg gtttaatggt tttttattat
     1981 cccccaaatt attactgcag atataaagct aaaaattcct agaaggaaag gaaaatcaag
     2041 cgaatttaaa agatcaatta ttgaatcaaa ttctgaattc atgttattgt tatttgtttt
     2101 aatttattat aatttgatac tcattattat taattagttg caaaaaaaat cttcactttt
     2161 ttgtctttat agttcttaat agatgtggat ataatcaatt aaaaaattga actaaaatct
     2221 gaattctatt aaaaatcaat aaaaacaatc tcgtatctgt tttagaattc ttgctgtagc
     2281 cccccaaatt atttcactat tatatctaaa tgtgtaggaa tcactaaatt ttttataatt
     2341 aaaattatta ttaaaaccct taaacaaatc aaatacagga atatccaata tttccgagac
     2401 ttcatcgatt aatattctcg tctttctaat actatcctga aaagtgacaa atggaattat
     2461 tgcaaaattc gaagttaatg ttctgactgt atggaaactt cccacgattt catttgaatg
     2521 aaaatttaga cctatttcct cttttgtttc ccttaatgct gtatttaata atgacgaatc
     2581 tgtttctttg aatgttcctc ctggaaatga aatttcattt ttgtgatact tcaacagaga
     2641 acttcttttt gttaagataa tttttgggtt aaaatctgta aagtgaataa ttacaagaac
     2701 agaagaaaga gtatatccag atgattttaa taattggaca tcgacattga ttttagatga
     2761 aagtctatga ataatctttt ctttgaattg gtttttatcg atatccaata atataaaata
     2821 gcggcaggaa tattttaaaa ttatagattt aataattatt aaaaaagtga atttgattta
     2881 gtacaagaat tgatagataa tgtaaaaaat aagccaaatg aaaaaacaat tagaaagact
     2941 aaagtagaac aattttcatt atactgtaaa taaatcaatt atattttata ttccattaga
     3001 cgaaaattaa gtgatactag ttgataatta attatgagtt aaatccacct aaaatattac
     3061 ctaatgatta ttttagttat tcaaatcttt taagcgatat agaaaatgta aaaaataagg
     3121 caatgatact tgatgaatat atcaattgtc ttcattttac tgattcagta ttaggggttc
     3181 ctagattatc aagtataacc atggcgcgca taataaaaga aaataatact aaaatgaaaa
     3241 tttcatgtag tgtaagaaca agagatagaa atttgacatc catgtatcaa actatttctg
     3301 attctatttt actaagtatt gaaagtttac tcattttact gggagatcaa tccaaaaatg
     3361 atttacagat tcatggatta ttaaaaccta gtaaagttgt taatcagttt aatgctcaag
     3421 a
//
LOCUS       DATAFW010000126         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2365491, whole genome shotgun sequence.
ACCESSION   DATAFW010000126 DATAFW010000000
VERSION     DATAFW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365491"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VHJ57_03005"
     CDS             <1..1297
                     /locus_tag="VHJ57_03005"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX2487532.1"
                     /translation="KIFELMKSHHDWFANSIPLIASENIPSTAVREAIVSDFGNRYAE
                     GWPGERVYAGCTFIDQVEIICNELARKVFNAEFADCRATSGVVANLAIYSGFTLPGET
                     MIATSIPNGGHISHGKKEHFGTAGHVCGLNIEFFPFSKEEMTIDVDLTKKKILDLINN
                     GKSPKMGMFGGSLFLFPHPVKELVDFMHDHKMFINYDGAHVAGLIAGGEFQDPLGEGV
                     DSMTMSSHKTLWGPQGGIILSLEKYAETIKKSIFPGNTSSHHLHHVAGKAIALAEVLE
                     FGKDYAKQVIKNAKKLAESLAGYGFTVLGEKRGYTQSHQIAVDVSKYNDGGTIEKDLE
                     KSNIILNRQLLPGDIQAGRHYMHPSGIRIGANEVTRLGMKESEMIEIANFIKRVVIDK
                     EDTNNIKRDVTEFKKQYQKLKYVFDGQLEAYKYISLVQV"
     gene            complement(1417..>2328)
                     /locus_tag="VHJ57_03010"
     CDS             complement(1417..>2328)
                     /locus_tag="VHJ57_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HEX2487533.1"
                     /translation="SSIDDLIGHIIVGLAPHTSVGIIGRIIGYTNSQVCLASPMWHSA
                     KRRDCDGDADSIMLLMDVFLNFSKEFLPDKIGGLMDAPLLIQPIVLPHEVQRQALNID
                     VTSNYLLQLYEKSLDYTKAENLSNKVDIIKDRIGSELQFYNYYFTHHTNLLTTEEKRS
                     AYSRLTTMNEKLDMQISTAKLINAVIPDEVASMVLTTHILPDIMGNMRSYSSQAFRCT
                     KCGEKYRRIPLAGKCLGCDNNLIQTVTRNSVEKYVYIALDIRNKFKMNNYLSSRIESL
                     VMELNYIFKEKEIQKNPQSSIIDYLDQ"
BASE COUNT          838 a          299 c          407 g          784 t
ORIGIN      
        1 taaaatcttt gagttaatga aaagccatca tgattggttt gctaattcta ttccattaat
       61 tgctagtgag aatattccta gtacagcagt aagagaggca atagtatctg attttggaaa
      121 tagatatgct gaaggatggc ccggtgaaag agtatatgct ggttgtactt ttattgatca
      181 agttgaaata atttgtaatg agcttgctag gaaagtgttt aatgcagaat ttgctgattg
      241 tcgtgcaacc tcaggagtag ttgcaaatct agctatatat tcaggattta ctcttccagg
      301 agaaacaatg atagctacat ctatcccaaa tggcggacat atttctcatg gaaagaaaga
      361 acattttggc actgcaggtc atgtatgtgg attaaacatt gaatttttcc ctttttcaaa
      421 agaagaaatg acaattgatg ttgatttgac aaaaaaaaag attttggatt taattaataa
      481 tggtaaatct ccaaaaatgg gtatgtttgg agggagttta ttcctttttc cacatcctgt
      541 aaaagaatta gtagatttta tgcatgatca taagatgttt attaattatg atggggcaca
      601 tgttgcagga ttaatagcag gaggagaatt ccaagatcca ttaggagaag gagttgattc
      661 tatgactatg agctctcata agacattatg gggacctcag ggaggaatta tactttcatt
      721 agaaaaatat gcagagacaa taaaaaaatc aatatttcct ggtaatacaa gtagtcatca
      781 cttacatcat gtagctggta aagcaatagc attagcagaa gtattggaat tcggaaagga
      841 ttacgctaag caagtcataa aaaatgctaa aaaattagca gaaagtttgg caggttatgg
      901 atttactgtg ttgggagaaa aaagaggtta tacacaatca catcagattg cagtagatgt
      961 ttcaaagtat aatgatggtg gaactattga aaaggatttg gaaaaatcta atataattct
     1021 taatagacaa ttacttccag gagatattca ggcaggaaga cattatatgc atcctagtgg
     1081 catcagaata ggggctaatg aagtaacaag attaggaatg aaagaaagtg aaatgataga
     1141 aatagctaat tttataaaaa gagttgttat tgataaagaa gatactaata atattaaaag
     1201 agatgttact gaatttaaga aacaatatca aaaactaaag tatgtttttg atggtcaact
     1261 tgaagcttat aaatatatca gcctagtaca agtctaataa ctatttttat attatattta
     1321 atttaatatt aatatagatt tattaattat ttaattaaaa agtatcattc tagataaata
     1381 aatttataaa gttaagagtt acaaataaat ttttatttat tgatctaaat aatcaataat
     1441 agacgattga ggattttttt gtatttcttt ttctttaaaa atatagttta attccataac
     1501 aagtgattct attctacttg aaagataatt attcattttg aatttattac gtatgtctaa
     1561 tgcaatatat acatatttct caacggaatt tcttgtgacc gtttgaatta aattattatc
     1621 acatccaaga catttacctg ctagtggtat tctacgatat ttttctccac atttagtaca
     1681 tctaaatgct tgtgatgaat aagaacgcat atttcccata atatcaggta gaatgtgagt
     1741 agtaagaacc atggatgcga cttcatctgg aataactgca ttaatcaatt tggccgttga
     1801 aatctgcata tctaattttt cattcatggt tgttaatcta gaatatgcac tacgtttttc
     1861 ttcagtagtt aataagttag tatgatgagt aaaataataa ttatagaatt gtaattcaga
     1921 accaattcta tctttaataa tatcaacttt gtttgaaagg ttttctgctt ttgtataatc
     1981 tagggatttt tcatataatt gtaatagata attcgaggta acatcaatat tgagagcctg
     2041 tctttgtacc tcgtgaggta aaacaatagg ttgaatcaat aaaggtgcat ccatcaaccc
     2101 tccaatttta tctggaagaa attctttaga aaaatttaag aatacatcca ttagtagcat
     2161 aatggaatct gcatcaccat cacaatccct acgtttagca gaatgccaca ttggagatgc
     2221 taaacagact tgtgaattgg tatatcctat aatccttcca atgattccca cggatgtatg
     2281 aggagcaagt cctactatta tatgacctat taaatcatct atagatga
//
LOCUS       DATAFW010000127         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1310506, whole genome shotgun sequence.
ACCESSION   DATAFW010000127 DATAFW010000000
VERSION     DATAFW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310506"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VHJ57_03015"
     CDS             <1..387
                     /locus_tag="VHJ57_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2487534.1"
                     /translation="MSTHTLTFSTPEMTLKKFAVWLAEIVENNGEEVPRIILYLQTRD
                     KENEEQSQSDETSINKPINFSDLLPDVDETFKPTGAIADMFNKTEPSFSKNIEYPTNP
                     ESKFCIECGNKLRTDSKFCTKCGAKF"
     gene            complement(614..883)
                     /locus_tag="VHJ57_03020"
     CDS             complement(614..883)
                     /locus_tag="VHJ57_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487535.1"
                     /translation="MAEANFRVQSHKFTQDNIQVTLVCSKCDEIFSKEFLLDLEGQTI
                     EFRCPVCGVSGEADLPYKTAEEREEFDDDLDEVEEDSFDEFEEDY"
     gene            complement(1017..1610)
                     /gene="folE"
                     /locus_tag="VHJ57_03025"
     CDS             complement(1017..1610)
                     /gene="folE"
                     /locus_tag="VHJ57_03025"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX2487536.1"
                     /translation="MTNSRVQKTGTINKNKIEKLIRQLLVELGENPNREGLIGTPKRI
                     TEMYEEIFSGYKMNSELDVSFSEETNAIIARDIQFYSMCEHHMLPFYGRIHIAYVPNG
                     KVFGLSKLVRLAEKYSRRMQIQERLTTEIANEISKRGVSGVIVIAEGEHLCMKMRGVR
                     NNNSIMTIAYRGVFEEKGIRENILNLIYTRQNNIINL"
BASE COUNT          605 a          294 c          193 g          690 t
ORIGIN      
        1 atgtctactc atactttgac tttttctaca cctgaaatga cacttaaaaa atttgcagta
       61 tggttagcgg aaattgttga aaataatggt gaggaagtac ctcgaattat actttatcta
      121 caaacaagag acaaagaaaa tgaagagcaa agccaatcag atgagacatc gattaataaa
      181 cctatcaatt tttcggatct tttacctgat gttgatgaaa ctttcaaacc cacaggagca
      241 atagctgata tgtttaacaa aacagaacca tcattttcta aaaatattga atatcctaca
      301 aatccagaat caaaattttg tattgaatgt ggtaataaac ttcgaactga ttctaaattc
      361 tgcacaaaat gtggtgcgaa attctaaaat ttaaatacat aataacagta aattaagctt
      421 ttttcattag ctatttctat tttccataaa ttctttttct tttattaata aataatactc
      481 attacaaatt gattgagaaa ttaaaattta acgaatagaa tctgatattt ttacattttt
      541 gacagatttg catatcttaa taatttaaat taaaaaataa catatgtaat aaatattgca
      601 tttattagat cctttaataa tcttcctcaa attcgtcaaa tgaatcttct tctacttcat
      661 ctaaatcatc atcaaactct tctctttctt ctgctgtttt ataaggtaaa tcagcttcac
      721 ctgaaactcc acaaacagga catctaaatt ctatggtttg tccttccaga tccaataaaa
      781 attccttgga aaatatctca tcacattttg aacaaactaa tgtcacttga atattgtctt
      841 gagtaaattt atgagactgg accctaaagt tcgcttcagc cattcttctt acctatagaa
      901 aatttacttc attataagcc tttcattata aaaatattat ggaaatttaa ataaaattta
      961 aataaaaaac aactttttat tttaatgtga ttaatatttt aagtaattta ttatttttat
     1021 aaattaatta tattattttg tctagtataa attaaattaa gaatattttc tcttattcct
     1081 ttttcttcaa atactcctcg atatgctata gtcattattg aattattatt tctaactcct
     1141 ctcatcttca tacaaagatg ttcaccttca gctataacaa ttactccact aactcctctt
     1201 ttagatattt catttgctat ttcagttgtt aatcgctcct gaatttgcat tcttcttgag
     1261 tatttctcag ctaatctaac taattttgat agtccaaata ctttgccatt tggaacataa
     1321 gcaatatgaa ttcttccata gaaaggaagc atatggtgtt cacacatgct ataaaattgt
     1381 atatctctag ctattattgc attagtttcc tcactaaaac taacatctaa ttctgaattc
     1441 attttatatc cagagaaaat ttcttcgtac atttcagtaa tacgttttgg tgttcctatc
     1501 aatccttctc tattaggatt ctctcctaat tcgaccaaaa gctgtctaat taatttctca
     1561 attttatttt tatttattgt tccagttttt tgtactctac tatttgtcat ctttagttat
     1621 cttgctccta ttatcttgtg taactgagga attatacgaa cttcgttata tattggataa
     1681 acaaggtcat aaaactgcaa taatctattc acagttggtt catttatcgt agaagttggc
     1741 tgtattataa atcctgctaa gtcatcacat tttatagtat ta
//
LOCUS       DATAFW010000128         1631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_606297, whole genome shotgun sequence.
ACCESSION   DATAFW010000128 DATAFW010000000
VERSION     DATAFW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606297"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /gene="thpR"
                     /locus_tag="VHJ57_03030"
     CDS             <1..560
                     /gene="thpR"
                     /locus_tag="VHJ57_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX2487537.1"
                     /translation="FNMRTFIAVDCNNKENIRKIQQMILKSVPNLNGGIKVIDPQNLH
                     FTLFFFGEVDEKTVEQIKNKMNEITFQGFEIIYKKIGVFPSFQFPKIIWIGLDDNSTK
                     KLTLIFELVKSKMEQIGFKPDKQFKPHLTLFRLKKPISNIKDYLPKDTDFIAGSDIID
                     KIHLKKSELFPSGPVYSNISTVFAK"
     gene            573..>1631
                     /gene="cca"
                     /locus_tag="VHJ57_03035"
     CDS             573..>1631
                     /gene="cca"
                     /locus_tag="VHJ57_03035"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR03671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX2487538.1"
                     /translation="MINMNNSNNNNNKIKSDAKDLEIDTITDKVLKTIKPSLKDFQKL
                     KAIENKIKDEIYSYKIPQIVDIKTGGSFAKDTNLKKDMDIDIFILIDKNTTELDFEKI
                     ALDVGFKSLKEYHPITRYSEHPYVEGYVFLNNNKEHVRLNVVPCYKVEQGQWKSSADR
                     SQYHTEYMMKILTPEHKNQIRILKAFLKGIGIYGAELSIAGLSGYVTEVLVLKYGNFK
                     NVIKNIAMVENKGKVIAIDNIPNNITEKFQSPIIIIDPVDSNRNLGSAISPESLSRFI
                     FGARVFLKKPAFNFFSINSKKDLINFDKDLQKLSELLQYILVLEFKFKSRSSDIIWGQ
                     LKKLTLSLARHLNDKEFEA"
BASE COUNT          652 a          203 c          251 g          525 t
ORIGIN      
        1 tttttaatat gagaacgttt attgctgtag actgtaacaa taaagaaaac attcggaaga
       61 tacagcaaat gatactaaaa tctgttccta atctgaatgg aggtattaag gttatagacc
      121 cacaaaatct ccattttaca ctgttttttt ttggtgaagt ggatgaaaaa actgtagaac
      181 aaattaaaaa taaaatgaat gaaataacct tccaaggctt cgaaataatt tataaaaaaa
      241 taggtgtatt cccatcattc cagtttccaa aaattatttg gattggttta gatgacaata
      301 gtacaaagaa attaacttta atctttgaat tagtaaaatc taaaatggaa caaataggat
      361 ttaaacccga caaacaattt aaacctcatt tgacattatt tcgtctaaaa aaaccgatat
      421 caaatattaa agattactta cctaaggata ctgattttat agcaggttca gatattattg
      481 acaagattca tttaaaaaaa agtgagcttt ttccttctgg tccagtatac tctaatattt
      541 cgacggtctt tgcaaaataa ataataaata aaatgattaa tatgaataat agtaataata
      601 ataataataa aattaagtct gatgctaagg atttagagat tgatactatt actgataaag
      661 ttttgaagac aattaagcct tcattaaagg atttccagaa attaaaagca atagaaaaca
      721 aaataaaaga tgaaatttat agttacaaaa taccacaaat agtagatatc aaaactggag
      781 gttcttttgc aaaagacaca aatcttaaaa aagatatgga tatcgatatt tttattttaa
      841 ttgataaaaa tacaactgag ctagattttg agaagatagc gctagatgtg ggtttcaaat
      901 ctttaaagga atatcatcct attacacgct attctgaaca tccttacgta gaaggttatg
      961 tttttcttaa caataacaaa gaacatgtaa ggttaaatgt agtaccatgt tacaaagtgg
     1021 aacagggtca atggaaaagc tctgctgata ggtcacaata ccatacagaa tatatgatga
     1081 aaatacttac acccgagcac aaaaatcaaa tcagaatctt aaaagccttt ttaaaaggta
     1141 ttggaatcta tggagctgaa ctatcaattg ctggattaag tggttatgtt actgaggttt
     1201 tagtactaaa gtatggaaat tttaaaaatg ttattaaaaa tattgccatg gtggaaaata
     1261 aaggtaaagt aattgctata gataatattc ccaataatat tactgaaaaa tttcaaagtc
     1321 caataataat aattgatcct gtagattcaa atcgtaactt gggatcggca atatctcctg
     1381 aaagtctctc gagatttatt tttggcgcga gagttttttt aaaaaaaccc gcatttaatt
     1441 tttttagtat taatagcaag aaagatttga taaattttga taaggattta cagaagttat
     1501 cggaactatt acaatatatt ttagttttag aatttaaatt taagagtaga agttctgata
     1561 taatttgggg ccagttaaaa aaattaacct tatcacttgc ccgacattta aatgataaag
     1621 agtttgaagc c
//
LOCUS       DATAFW010000129         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4659710, whole genome shotgun sequence.
ACCESSION   DATAFW010000129 DATAFW010000000
VERSION     DATAFW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659710"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VHJ57_03040"
     CDS             <1..1291
                     /locus_tag="VHJ57_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX2487539.1"
                     /translation="IGIYKMAMVDTNQDPACMAANACLKLMQKNDLKPQDIGRMYVAT
                     ESALDESKAMNSFVIGMLEQIYGDGSFEHAGGIECKFACVSGSYALYDNANWIRAEEN
                     NDRAAIVIVSDIAKYDLGSAGEYTQGAGAIAMLVKENPRLVIFDPKVTSTIIKNEYDF
                     YRPFGRETPLVNGNYSNYLYLIQVKKAFDSYREKAIKTKLIQLQEDESITDKIDYISV
                     HLPYRKMGEKALAYLLRHEWRCLPRWKQIIEEVGMDEPTPKDPRGTIESILADKDFMK
                     ADETFRKAFMRTSFFKQVFEQKMASSLEASAIIGNLYTGSMYMGLRSLLEFEYKKNID
                     LENKRIGFGSYGSGSSAMVFSGVIQSTYKEIVKDMNLEREIGERSKLDMQEYIRLHSN
                     EGKMNEPILNSNNEFVLVKIGGNTDYNAGLREYVFSN"
     gene            complement(1284..1784)
                     /locus_tag="VHJ57_03045"
     CDS             complement(1284..1784)
                     /locus_tag="VHJ57_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX2487540.1"
                     /translation="MSIIDGFIIWIHLLSASIWVGGSIFIGIILAPMLKHIGNSLEER
                     VIIMIKIGRRFNNIAFPSFIILMSTGLYNSRFFFEYPDNFIETDYGILLLVKIVLVII
                     TFASYIIHIKSFGKETEAKIIEYRGSEYINIIRSKIINYGRLTVILSIIILLLAAFLD
                     TGIKFS"
     gene            1915..2430
                     /locus_tag="VHJ57_03050"
     CDS             1915..2430
                     /locus_tag="VHJ57_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487541.1"
                     /translation="MFLSKRRLVLFGVIGALVITIIFLPQIQYFVNAPDVNVIDFNLA
                     AVNISNLSNNTNNIELSLLLDVYNPSDKSVTTSKVEYDLFGDNKFLGHGVLSYEDIPL
                     NGRPQFSPGQTTTLKSSIILQPSDMNMDVIENLISNSSDSINSIRWNVNGSSQIESAF
                     VILEKDFSDVL"
     gene            complement(2473..>2722)
                     /locus_tag="VHJ57_03055"
     CDS             complement(2473..>2722)
                     /locus_tag="VHJ57_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487542.1"
                     /translation="ERMLYKGRWTYKLIVKKIPQLKEANRRPIEIASVEGAPCFSCTF
                     QHLCSTEDETSQYSPAKCNWIEQWVILDTKKRGLSNQL"
BASE COUNT          968 a          375 c          433 g          946 t
ORIGIN      
        1 aataggaata tataaaatgg ctatggttga tactaatcag gatcctgctt gtatggcagc
       61 aaatgcttgt ttaaaattaa tgcaaaaaaa tgatcttaaa ccacaggata ttggtagaat
      121 gtatgttgca actgaatcag ccttagacga atcaaaagct atgaattctt ttgtaattgg
      181 aatgcttgaa cagatttacg gtgatggatc ctttgaacat gctggaggaa tagaatgtaa
      241 gtttgcttgt gttagtggct cctatgcatt atatgacaat gcaaattgga tcagagctga
      301 agaaaataat gatagagctg ctattgttat tgtaagtgat attgcaaaat atgatttagg
      361 ttcagctgga gaatatacac agggagctgg tgcaatcgct atgttagtaa aggaaaatcc
      421 tagattagtt atttttgatc ctaaagttac ttctacaata attaaaaatg aatatgattt
      481 ttatagacct tttggacgtg aaactccttt agttaatgga aattattcta attatttgta
      541 tcttattcaa gttaaaaaag cctttgatag ttatcgagaa aaagcaatta aaactaaatt
      601 aattcaatta caagaagatg aatcaataac cgataaaatt gattatatta gtgtacattt
      661 accttatcga aaaatgggtg aaaaggcatt agcatatctt ctacgacatg aatggagatg
      721 tctacccaga tggaaacaaa taatagaaga agttggaatg gatgaaccta ctcctaagga
      781 tcctcgtgga acaattgaat ccattttagc cgataaggat tttatgaaag cagatgaaac
      841 tttcaggaaa gcgtttatgc gtacttcctt cttcaaacaa gtttttgaac aaaaaatggc
      901 tagctctctt gaagcttcag ctataatagg taacctttac accggatcca tgtatatggg
      961 attaagaagt ttattagagt tcgaatataa gaaaaatatt gacttggaaa ataaaagaat
     1021 aggatttgga tcatatggaa gcggaagtag cgcgatggtc tttagtggtg ttatacaatc
     1081 tacgtataag gaaattgtta aagatatgaa tttagaaaga gaaataggcg agcgttctaa
     1141 attggatatg caggaatata tccgtcttca ttcaaatgaa ggaaaaatga atgaaccaat
     1201 tttaaattct aataatgaat ttgttttagt aaaaataggt ggtaatactg actataacgc
     1261 tgggttaaga gaatatgttt tttctaacta aattttatac ctgtatccaa gaaagccgca
     1321 agtaacaaga ttattataga aagaatcaca gttaatcttc cgtagttaat aatttttgaa
     1381 cgaataatat taatatattc tgatcctcta tattcaataa tttttgcttc tgtctctttt
     1441 ccgaaacttt taatatgtat aatataactt gcaaaagtga taattactaa tacaattttc
     1501 accaacaata aaattccata atctgtttca ataaaattat ccggatattc aaaaaaaaat
     1561 cttgaattat acaatcctgt tgacatcaaa ataataaatg aaggaaatgc aatattgtta
     1621 aatcttctac caattttaat cataataata actctttctt ccagtgaatt tccaatatgc
     1681 tttaacatgg gagcaagaat tattcctata aagattgatc ccccaaccca aatacttgca
     1741 gataataagt gaatccatat aataaagcca tcaattatac tcatagatag ttcaacagta
     1801 tattagttta aaccatctaa tcactttaat gtatctttat agtgttatag tgctatatga
     1861 taatcaaaat taagaaacat tttaattctt ttttggaaac tttacttgta atatatgttc
     1921 cttagtaaaa gaagactagt actttttgga gtaataggtg ctttagtgat tacgattatt
     1981 tttcttccac aaatccaata ttttgttaat gctcctgatg tgaatgtaat tgattttaac
     2041 ctagcagctg ttaatattag taatctttca aataatacta acaatattga gctatcctta
     2101 cttttggatg tttataatcc ttctgacaaa agtgtaacaa catcaaaagt tgaatatgat
     2161 ctttttggag ataataaatt cttaggtcat ggcgttttat cttatgaaga tattccatta
     2221 aatggtaggc cccaattttc tcctggacaa accactactt taaaatcttc tattattctt
     2281 caaccatctg atatgaatat ggatgtaatt gaaaacttaa taagtaattc atcggattca
     2341 ataaatagta taagatggaa tgtgaatgga tctagtcaga tagaatctgc atttgttatt
     2401 ctagaaaaag atttcagtga tgttctttaa tttttagata gataataaaa aataatgaat
     2461 tttttgttaa tgctatagct ggtttgatag tcctcttttt tttgtatcaa gaataaccca
     2521 ttgctcaatc caattacatt ttgcagggct gtattggctt gtctcatcct ctgtagaaca
     2581 taaatgttga aaagtacaac taaaacatgg agctccttct acactagcaa tctcaattgg
     2641 tctcctattt gcttctttta attgaggtat tttttttact attaatttat aagtccacct
     2701 gcctttgtat aacattcttt ct
//
LOCUS       DATAFW010000130         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4835657, whole genome shotgun sequence.
ACCESSION   DATAFW010000130 DATAFW010000000
VERSION     DATAFW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4835657"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2817)
                     /gene="polC"
                     /locus_tag="VHJ57_03060"
     CDS             complement(<1..2817)
                     /gene="polC"
                     /locus_tag="VHJ57_03060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HEX2487543.1"
                     /translation="MNVHYNFLDENSLQELSNLKRPPFYNNYHNTLLNFLNEIYLIAS
                     NARSQGIDVSEIIESRIVYDLADRVAKMHNINITNRLRNLLSETTKEKAALKISEEIA
                     QGKYSDDDLTTRLDNAVRVSLAIVTEGVTVAPLQGISDVQIKYNSNGTQYLSISFAGP
                     IRSAGGTEAALTMLIADHVRKSVGLSKYQANSFDDETGRFVEELRIYEREVGSFQFKI
                     LDEDVIKCISNLPVELDGVDTDPVEIVGHRAMKRIKTDRVRGGALRVMNDGLIGRSRK
                     LLKIIDTLGLDGWDWLKELKGAIQTEGSSEDDTIHHRMSEVITGRPVLSMSNKIGGFR
                     LRYGRCYNTGFSTIGIHPTVPILLNYAIVVGTQIKIDIPGKAATIALVDSIEPPLVKL
                     DDGTVTYVTSIEHAHQIQNRIVTILYLGDILVSYGDFLENNAKLFPASYVEEIWGLEL
                     QMKIKQISSNNSYSTDEKYTNKILEYIDKQRLFELANNSIEIIPTLKEAFLISKMLSM
                     PLHPKYSFYWESISVDEIISLRNEFFKKSDIDKNVDLIFNMTKTTTNNQYYYESFSIE
                     YNGILKDIMERLGITHSVKNYNNNLFIELSEINQINSLFILLCNTSINNESSSNKISS
                     IDYIMRISGLLLKPKFSSSIAVRVGRPEKAAERKMKPPVHVLFPVGSKGGSTRDLIKA
                     SKEEILYTEIANRYCKNCNLPSVGTKCRNCLSETPLTNICGICRELINSENNSKKEVT
                     TRCTKCRREGKTYSPTKYPLKKMLKEAEKTLEIQFKEPLKGVKILMGKNKSAEPLEKG
                     ILRQNYDLYAFKDGTIRYDATNGPLTHFKPKWIMTNIDKLRNLGYSKDFHGKDLSTDD
                     QLLEIFIQDVILPIETANHLLRLSRYIDKELLKLYKLEPFYNASSIDDLIGHLIVGLA
                     PHTSVGIIGRIIGYANSQVCLASPM"
     gene            complement(2814..3551)
                     /locus_tag="VHJ57_03065"
     CDS             complement(2814..3551)
                     /locus_tag="VHJ57_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEX2487544.1"
                     /translation="MNTIETYILNEIKRYRTLCFPLIDSENPKDVISIAKKVEQLGAS
                     AILIGGSSIIDQIELSKIISQIKKSITIPVILFPGNVTGVSPNADAILFMSLLNSENP
                     YFITGAQALAAIGVKKSKLEALPTGYLIIGEGTTAWFVGQAKGIPFHKPNLAIMYSLA
                     AQYLGMRFLYLEAGSGANQHISPEMVKSVRKYYEGILIVGGGIRDSEIAKHIANAGAD
                     IIVVGTMIENDNNWEKKFSEIIQLIRK"
     gene            3740..4144
                     /locus_tag="VHJ57_03070"
     CDS             3740..4144
                     /locus_tag="VHJ57_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487545.1"
                     /translation="MYGTDRKDGLEFNDLLNRLIEKNLFTKKQISIIYNKVNLHTSNV
                     SNDQLSNQLSAGAYYRQVKQCRKKYDRFIYTILLFRLLNLIDNTSIQTIESMVNQLEK
                     ISRHDIHHNSPEIVSNDIIVVIEEILRRINKV"
BASE COUNT         1380 a          693 c          573 g         1643 t
ORIGIN      
        1 cattggagac gctaaacaga cttgtgaatt agcatatcct ataatccttc caatgattcc
       61 cacggatgta tgaggagcaa gtcctactat taaatgacct attaaatcat ctatagatga
      121 ggcattataa aatggttcaa gcttatataa ttttaatagc tctttgtcta tatatcttga
      181 tagtcgaagt aaatgatttg cagtttctat aggtaatatc acatcttgaa tgaaaatttc
      241 taataactga tcatcagtag ataaatcttt tccatgaaaa tctttagaat aacctagatt
      301 ccttaattta tctatattag tcatgatcca cttaggttta aaatgtgtta atggtccatt
      361 agttgcatca taacgtattg taccatcttt gaaagcatat aagtcataat tttgccttaa
      421 aattcctttt tctaatggtt cagcggattt attttttccc atcaaaatct ttacaccttt
      481 taatggttct ttaaattgta tttctagagt tttttctgct tcttttaaca tttttttaag
      541 aggatactta gttggagaat atgtttttcc ttcccttcgg catttagtac atctagtagt
      601 tacttctttt ttactattat tttctgaatt aattagttct ctacagattc cacaaatatt
      661 agtcagaggt gtctcagata aacagtttct acatttagta ccaacggatg gtagattaca
      721 atttttacaa tatctattag ctatttctgt ataaagaatt tcttcttttg atgctttaat
      781 taaatctcgt gtagaacctc ctttacttcc tactggaaat aagacatgaa ctggcggctt
      841 catttttctt tcggctgctt tttctggccg cccaacacgt acagctattg atgaagaaaa
      901 ttttggtttt agtaaaaggc cagatattct cattatataa tcgattgaag aaattttatt
      961 tgatgatgat tcattattta tcgatgtatt acaaagtaaa ataaacaaag agtttatttg
     1021 attgatttct gacaattcta taaatagatt attattataa ttttttactg aatgtgttat
     1081 acctaaacgt tccattatat cttttaatat tccattatat tcaattgaaa aagattcgta
     1141 ataatactga ttattagtgg tggtttttgt catattaaat attaaatcaa cattcttgtc
     1201 aatgtctgat tttttaaaga attcatttct taatgaaata atttcatcca ctgatattga
     1261 ttcccaatag aaagaatatt tgggatgtaa aggcatactc aacattttac taattaagaa
     1321 tgcttctttc agtgttggaa ttatttcaat agaattattt gctaattcaa ataatcgttg
     1381 tttgtcaata tattctaaaa ttttattagt atatttctca tcagtggaat aactattatt
     1441 tgaagaaatc tgctttattt tcatttgtaa ttctaatccc catatctctt caacatagga
     1501 tgcaggaaat aattttgcat tattttctaa gaaatcacca taactaacta aaatatctcc
     1561 caaataaagg atagtaacaa ttctgttttg aatttggtgg gcatgttcta tagatgtaac
     1621 ataagttaca gttccgtcgt ctagtttcac taaaggaggt tctattgagt caactaatgc
     1681 gattgttgct gctttcccag gaatatcaat tttaatttga gttcctacta ctattgcata
     1741 atttaataat ataggaacag ttggatgaat ccctattgtg gaaaatcctg tattatagca
     1801 tctaccatat cttaacctaa atccaccaat tttattagac attgacaaaa ctggtctacc
     1861 agttattact tccgacattc tatggtgtat tgtatcgtct tcactagaac cttctgtctg
     1921 tattgctcct ttcagttcct taagccaatc ccatccatct aatcctaaag tatctattat
     1981 ttttagtaat tttctacttc ttccaattaa accatcattc ataactctta gagcaccacc
     2041 acgtactctg tctgtcttga tccttttcat tgctctatgg ccgacaatct ctactggatc
     2101 agtatctact ccgtctaatt ctactggtaa attactaata cattttatta catcctcatc
     2161 taaaatttta aattgaaagc ttccaacttc tctttcatat atacgtaatt cctctacaaa
     2221 tcgacctgtc tcatcatcaa aggagttggc ctgatattta cttaacccaa ctgactttct
     2281 tacatggtct gcaataagca ttgttaaggc tgcttctgtt cctccagcag atctaattgg
     2341 tccggcaaat gatatggaaa gatattgagt accattagaa ttatacttga tttgtacatc
     2401 tgatatccct tgtaaaggtg caacagttac tccttctgtt actatagcaa gacttactct
     2461 aactgcatta tctaatctag ttgtcaagtc atcatcgctg tatttccctt gtgcaatttc
     2521 ttccgatatc tttaatgctg ccttttcttt agttgtttca gataataaat ttcttaatct
     2581 atttgtaata ttgatattat gcattttagc aactctatca gctaaatcat agactattct
     2641 tgactcaatt atttcagaaa catcaatacc ttgtgaccgt gcattactgg ctattagata
     2701 aatttcattt aaaaagttta agagagtatt atgatagtta ttataaaaag gcggtctttt
     2761 taaattgctt aactcttgaa gtgagttttc atctaaaaaa ttataatgaa cattcatttc
     2821 cttattaatt gaataatctc agaaaatttc ttttcccaat tattatcatt ttcaatcatt
     2881 gttcctacta ctataatatc agcaccagca ttagctatgt gtttagcaat ttcagagtct
     2941 cgaatgcctc cacctacaat taatataccc tcataatatt tccgcactga tttgaccatt
     3001 tctggagata tatgttggtt agcacctgaa cctgcctcta gatacaagaa tcgcattcca
     3061 aggtattgtg ctgccaggga atacattatt gccaagttag gtttatgaaa aggaataccc
     3121 tttgcttgac ctacaaacca tgcagtcgtt ccttcaccaa ttatcagata tccagtaggc
     3181 aatgcttcta acttggattt ctttacccca atggctgcta atgcttgtgc cccggtgata
     3241 aaatatggat tttcagaatt aagtaatgac ataaataata tagcatcagc atttggtgaa
     3301 actccagtga catttccagg aaataatatc acagggatag tgatactttt ttttatttgt
     3361 gatattattt ttgataattc aatttggtcg attatagatg aaccaccaat taaaattgct
     3421 gatgcaccta attgttcgac ttttttcgct attgaaatga catctttagg attctctgaa
     3481 tctattaatg gaaaacataa agtacgatat cgttttatct catttaaaat ataagtttct
     3541 atagtattca attactgtgg aaccatatta aagtatatta actcctaaaa aatatttttg
     3601 gttttgattt tatatatccc cagatctttt agcatataac tttaaattat tatagtaaaa
     3661 ctaagtaaaa taaaattttg actaccaagt tattattaat tagtttaatc tttttataag
     3721 aatctttgac tatacaaata tgtatggtac tgatagaaaa gatggtttag aatttaatga
     3781 tcttttgaat cgacttattg aaaaaaattt atttacaaag aagcaaatat caataattta
     3841 taataaagtc aatcttcata cttctaatgt ttctaatgat caattgagta atcaattgag
     3901 tgctggtgct tattatagac aagttaaaca atgtaggaaa aaatatgata gattcattta
     3961 tacaatacta ttgtttcgat tattaaattt aatagataat acttcaatac aaaccatcga
     4021 atcaatggtt aatcaactag agaaaatatc acgacatgac attcatcaca attcacctga
     4081 aattgtttca aatgatatta ttgttgtgat agaggaaata cttagaagga ttaataaagt
     4141 ctagtatgaa atgcattcat aatcgataaa aataaatata ttgtgatata tgtgatatca
     4201 tttgaatata ggtttggatg tattttcctt tatacttata ggaacctttt ttataattgg
     4261 atttttaatt tcctatattc tgaaaatac
//
LOCUS       DATAFW010000131         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6070579, whole genome shotgun sequence.
ACCESSION   DATAFW010000131 DATAFW010000000
VERSION     DATAFW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6070579"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VHJ57_03075"
     CDS             complement(<1..593)
                     /locus_tag="VHJ57_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX2487546.1"
                     /translation="MLLSFSSNAFKKFSLDTAIREIAKIGYKGVEILCDLPHAYPPEF
                     SKEQAKKIKNLISNLNIGISNLNAFTLYAVGDVYHPSWIENDNNLREIRVKHTIDCIY
                     LAKKLGAKNLSTEPGGPINIKKNKIESNIQLKIFADGLKKVKSIAEENDVKILVEPEP
                     SLLIENSQQFLQLMKTISSDYVKLNFDIGHFYCVKEDP"
     gene            complement(633..1454)
                     /locus_tag="VHJ57_03080"
     CDS             complement(633..1454)
                     /locus_tag="VHJ57_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX2487547.1"
                     /translation="MSGFFDPHIHMYSRTTDDYDAMSKAGIEVIVQPSFWLGGPRTFV
                     GTFIDYWEHLISFETTRAKQFGIEHFVCISVNPKESVQRPLALDALQAMLKFIDRERV
                     VAIGEIGYNLINELEEEIFLKQLDIATIKKIPIMIHLPHQNKTKGIERIEEILNQNSN
                     KYDRNKILIDHNTEETIKKTLEMGLWAGLSVYPLTKLTPKRAMDIIRKNGSDSIMINS
                     AADWGISDPLSVPLVAREMRKEGFKKNEIEKVTMFNAYDFYKQSDKFTWNPQIQC"
     gene            1834..>2102
                     /locus_tag="VHJ57_03085"
     CDS             1834..>2102
                     /locus_tag="VHJ57_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487548.1"
                     /translation="MKNNSSSTTTTTTNIKSTYNNSSSTMLSIDLAGIHLRNPLLLGS
                     GFLGISQQVFNRLYNDGLGAIVSKSISVSPLDGYKGPTVVSLGEKG"
BASE COUNT          686 a          314 c          272 g          830 t
ORIGIN      
        1 ggatcttctt tgacacaata aaaatgtcca atatcaaaat ttaattttac atagtcagaa
       61 cttattgttt tcattaattg taaaaattgc tgagagtttt caattaggag agaaggttct
      121 ggttcaacta atatttttac atcattttct tctgcaatag atttgacttt ttttaaccca
      181 tctgcgaata ttttcagttg gatatttgat tctattttat tcttttttat attaattgga
      241 cctcctggtt ccgttgaaag gtttttcgca cctaactttt ttgccaaata aatgcaatct
      301 attgtatgtt taaccctgat ttctcgtaaa ttattatcat tttcaatcca tgaaggatga
      361 taaacatctc ctacggcata taatgtaaat gcattaaggt tagaaattcc tatattcaaa
      421 tttgaaatca aattttttat tttttttgct tgttcttttg aaaactctgg aggataagca
      481 tgtggtagat cacataaaat ttctactcct ttataaccta ttttagctat ttctcttatg
      541 gcagtatcaa gggaaaattt cttaaaagcg tttgaactaa atgatagtaa catttctaac
      601 ttagcagtta tgaatatatt gtagttaata ttctaacact gaatttgagg attccaagta
      661 aatttatctg attgtttata aaaatcatat gcattaaaca ttgtaacttt ttctatttca
      721 ttttttttaa atccttcttt tctcatttct ctagcaacta atgggacact caatggatcg
      781 gaaatacccc aatcagctgc actatttatc attatactat ctgatccatt ttttcttata
      841 atatccattg ctctctttgg tgtaagtttc gttaaaggat aaaccgaaag acctgcccac
      901 aatcccatct ccaatgtttt ttttattgtt tcttccgtat tatgatcgat cagaattttg
      961 tttctatcat atttattact attttgattt aatatttctt caatcctttc tatcccttta
     1021 gttttatttt gatgaggtaa atgaatcata ataggaattt ttttgatggt tgcaatatca
     1081 agctgtttta aaaaaatctc ttcttctaat tcgttaataa ggttgtagcc tatttcacca
     1141 attgctacaa ctctttctct atctataaac tttaacatag cttgtagtgc atctaacgct
     1201 agtggtctct gtacagattc tttaggattt acggaaatgc atacgaaatg ttcaattcca
     1261 aattgtttag ctcttgtagt ttcgaaagat ataaggtgtt cccaataatc aataaacgtt
     1321 ccaacaaaag ttcttggacc tcccagccaa aaagagggtt gtactataac ttctattcct
     1381 gccttagaca tagcatcata gtcatcagta gttcgagaat acatatgaat atggggatcg
     1441 aagaatccag acatttactt ataaaattat ccaagttata ttaaatgtat atcttgtaat
     1501 attctcaata caatttcatg atattatatg gtgtagtttt ttatttatca taattatcag
     1561 ttatcttaga ataatgtata tcttataatt gggtaaaaat tataagaaat attatctata
     1621 cttaattatt tatttttgat gtatttattg tctaatataa agtaaaatgt aattacattt
     1681 acatgttaat atttagtatt ttttaaaaaa tgaaaaattg aaatttattt tgaatataaa
     1741 ctaaaaattt ttgattaaaa tttaatatat ttttacacaa ataaatctaa aacgaacaac
     1801 taataagata ttaaatcata agacaactag ccattgaaga ataattcaag ttcaactact
     1861 actactacta ctaatattaa aagtacttat aataactcct catctactat gttgtcgatt
     1921 gatcttgcag gaatacatct tagaaatccg cttcttcttg gatcaggatt tttaggcatt
     1981 tctcagcaag tttttaacag attatacaat gatggtttag gggctatagt cagcaaatca
     2041 ataagtgtta gtcctctaga tggatataaa ggtcccacag ttgtttctct tggagaaaaa
     2101 gg
//
LOCUS       DATAFW010000132         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2018578, whole genome shotgun sequence.
ACCESSION   DATAFW010000132 DATAFW010000000
VERSION     DATAFW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018578"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VHJ57_03090"
     CDS             complement(<1..635)
                     /locus_tag="VHJ57_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX2487549.1"
                     /translation="MQKNLGINFELKLPLILIHTPFGLRFFDTVANSRIGKTYASFNI
                     YLMPLITALAVFLILGSLLVLFSNDSAREGIREIGPQGNLLIPGLNPILPWTYGWIGL
                     VITIIIHEAGHGIVARVYKTKVESTGIVLFLGIPIGAFVNIQQDELNKTTFKQKSAIL
                     TAGPLNNMVLAGISFGLLFLLISSLTPVSHIQGDETGITVLTIGENSLAQS"
     gene            732..1391
                     /locus_tag="VHJ57_03095"
     CDS             732..1391
                     /locus_tag="VHJ57_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX2487550.1"
                     /translation="MDSTILNFNFFLRDTDLVAKKLIGQKLVKIIRTNNEKIKLSGII
                     VETEAYGYKSDEASHAYRGMTKRNIVMFGPVGRSYIYFTYGNHYCFNVTAKSKNQIAG
                     AVLIRGLEPTEGMEYMKKYRNLDDISLLTNGPGKITQAFCIDKKHNNYDLTNINNDIY
                     IIKGFIPNLVLATRRIGIKKSIHLYRRYVLASKIGNKIYINKFVSKKKENNHFQICTL
                     L"
     gene            complement(1386..>2451)
                     /locus_tag="VHJ57_03100"
     CDS             complement(1386..>2451)
                     /locus_tag="VHJ57_03100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487551.1"
                     /translation="NFPRVSIIVPARNEETYLRKCVDSLVNQDYPDYEIILVNDESSD
                     KTLDIMNEYKNSNSIIKVVNVNQPGGHWIGKNWACYQGYMKSNGNLLLFTDADSYHAE
                     NTLSLAVQNINHNKLDAITVMPRLICKDFFTKVTLPILSTFLHTRFSPLKVNDIKSKL
                     GYFFGCYFIIRRDTYEKVGSHAAVKHEIIEDGALGKKVKEENFKIKMLRGENYVQAIW
                     ARNSSELFTAIDRLIIPLFMENRVTSTLVGIAMFFLLLFPFVMFYLSLTLSVIHPIAV
                     DFVVLLLTGSTLFILLLTNLIQLRNTLYLNQIYSLGVILGATIIVSRFFSKLLIPNSK
                     TNVNWRDRLYKIPSNQTSVL"
BASE COUNT          917 a          371 c          374 g          789 t
ORIGIN      
        1 atcgattgag ctaaagaatt ctctccaata gttaaaacag taatacctgt ttcatcaccc
       61 tgaatatgag acacaggagt aagagaagag atcaaaagaa aaagtaaccc aaaggaaata
      121 cctgccagga ccatgttgtt caatggacct gcagtaagaa ttgcactttt ttgtttaaaa
      181 gttgttttat ttaattcatc ttgttggata tttacaaatg caccaatagg gatccctaaa
      241 aacaaaacaa taccagtcga ttcgactttt gtcttataaa ctctggcaac tataccatgt
      301 ccagcttcat gaataataat agtaattact aatccaatcc agccatacgt ccatggtaaa
      361 ataggattca atcctggaat caatagattg ccttggggtc caatttcgcg aataccttcc
      421 cttgcagaat catttgaaaa taggacgaga agggaaccca aaattaaaaa aacagctaat
      481 gctgttatta aaggcattaa ataaatattg aatgaagcat aagtcttacc tattctagaa
      541 ttcgctactg tgtcaaaaaa ccgtaatcca aatggggtat gaattagtat taacggcaat
      601 ttgagctcaa aattaatccc taagtttttt tgcaaacaaa attattacta ccagagacta
      661 tttaaatcaa tcattgatga cagtactact tctaaaatct attaataatt ttatttacat
      721 cattaaatta attggattcg acaattctaa attttaattt ttttttaagg gataccgatt
      781 tggtagcaaa aaagcttatt ggacaaaaat tggtaaagat aataagaact aataatgaaa
      841 aaattaagtt atccggaatc attgtagaaa cagaggcgta tggttacaaa agtgatgaag
      901 caagtcatgc ctatagagga atgaccaaaa gaaatattgt aatgtttggt cctgtaggaa
      961 gatcctatat ttatttcaca tatggtaatc attattgttt caatgtcact gcgaaatcta
     1021 aaaatcagat tgctggtgct gtattaatac gaggattaga accaaccgaa ggaatggaat
     1081 atatgaaaaa atatcggaat ttggatgata taagtctttt aacaaatggt ccaggaaaaa
     1141 tcacccaagc attctgcata gacaaaaaac ataataatta tgatttaacg aatataaata
     1201 atgatatata tattataaaa gggtttattc ctaatctagt attagcaact agaaggattg
     1261 gtataaaaaa gtcaatacat ctttatagga gatacgtttt agcctccaaa ataggaaata
     1321 aaatttatat aaataaattc gtatccaaaa agaaggaaaa taatcatttt caaatttgca
     1381 cactattata gtactgaagt ttgatttgat ggaattttat atagtcgatc cctccagttt
     1441 acattagttt tgctatttgg tattaataac ttagagaaaa atctagaaac aataattgtt
     1501 gcacctagta taacacctag agaataaatc tggtttaaat ataatgtatt acggagctga
     1561 attaaatttg ttagtaataa aataaacaat gtactaccag taagtagtaa gactacaaag
     1621 tcaactgcaa tgggatgaat tacacttaaa gttaatgata aataaaacat tacaaatgga
     1681 aaaagcaata aaaaaaacat tgcaataccc actagagttg atgtgactct gttttccata
     1741 aagagaggta taatgagtct atctatagct gtaaataatt ctgaggaatt tcttgcccat
     1801 atcgcttgca catagttttc acctctaagc atttttattt taaaattttc ctcttttacc
     1861 tttttaccta atgcaccatc ttcaataatt tcatgtttta ctgctgcatg agaaccaact
     1921 ttttcgtatg tatccctcct gataataaaa taacaaccga agaaatatcc aagctttgat
     1981 tttatatcat taacctttaa tggagaaaat cttgtgtgca gaaaagtaga taatatagga
     2041 agcgtgactt tagtaaaaaa atctttacat attaaacgag gcataactgt tattgcatct
     2101 aatttattat gattaatatt ttgtacagct agagacaatg tattttcagc atgataagaa
     2161 tctgcatcgg tgaacaacaa taaattacca ttagatttca tataaccttg atagcatgcc
     2221 cagtttttcc caatccaatg gccaccaggt tgattaacat tcacaacttt tatgatggag
     2281 tttgagtttt tatattcatt cataatatct agagttttgt cagatgattc atcattaact
     2341 aaaattattt catagtcagg ataatcttgg tttactaagc tgtctacaca tttacgaaga
     2401 tatgtttcct catttctggc aggaactatt atactaaccc gtggaaagtt g
//
LOCUS       DATAFW010000133         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4133769, whole genome shotgun sequence.
ACCESSION   DATAFW010000133 DATAFW010000000
VERSION     DATAFW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133769"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VHJ57_03105"
     CDS             complement(<1..704)
                     /locus_tag="VHJ57_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger domain-containing protein"
                     /protein_id="HEX2487552.1"
                     /translation="MRCHLCNGMDFLFKCNYCKELFCRDHRLPISHFCSAIDRFSDKR
                     KTNLFNDNFEKSGFLNNFTKTVNIRFSRTEIKHLLLATFLVLLVGMSLNNYRYISFEF
                     LIIFIIAFLFHELAHKFLANFYGSWAEFRAELYGLIVTAISAIPFIPFKFIAPGAVIV
                     GISEKEKLGKVALIGPMTNLVMGFLFLLLSYLYPYSLYFNIGASFNAWISMFNLLPFG
                     VLDGQKIFNWNKVVWSS"
     gene            825..1160
                     /locus_tag="VHJ57_03110"
     CDS             825..1160
                     /locus_tag="VHJ57_03110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX2487553.1"
                     /translation="METTNIEKFQNRKKTAIRLLAFLEAKKGKELELETILISLIRPT
                     LDEPGNIAYVPHRSLENPLFFMFDELWVDEKAIEEHFMQPHMHNLIEKLKPLLEKPLE
                     LKRYYEIIL"
BASE COUNT          641 a          224 c          217 g          510 t
ORIGIN      
        1 gaagaccata ctactttatt ccaattaaaa attttttgtc catctaatac tccaaatggt
       61 agtaaattaa acatagatat ccatgcatta aaactggctc ctatattaaa ataaagtgaa
      121 tatggatata aataggaaag caaaagaaat aagaatccca ttactaaatt agtcataggt
      181 cctatcagag caacttttcc aagcttttct ttctctgaaa taccaacaat aactgcacct
      241 ggggcaataa atttgaaggg aatgaatgga atagcagata ttgccgttac aattaaacca
      301 tatagttctg ctcgaaactc agcccaactt ccataaaaat ttgctaaaaa tttatgggct
      361 agttcgtgaa atagaaatgc tattataaaa atgatcaaaa attcaaatga gatatagcgg
      421 taattattca atgacattcc tactaacaat accaaaaaag ttgccaaaag tagatgtttt
      481 atttctgttc tagagaatcg aatattgaca gttttagtaa aattattaag gaaaccactt
      541 ttttcaaaat tatcattgaa tagattcgtt tttcttttgt cagaaaatct atcaatagca
      601 ctacagaaat gactaattgg aagacgatga tcgcgacaaa ataattcttt acaataatta
      661 catttaaaaa gaaaatccat tccattacat agatggcatc tcataaatca taatctaaaa
      721 agcaagatat atgattgccg attagaacta ctctataacc tacgatattt ataattctta
      781 aaaggttcaa taaaaaatat ttatagggta aaaaaatatc aaatatggaa actactaata
      841 ttgaaaaatt tcaaaataga aagaaaacag ctatcaggtt actcgcgttt ttagaggcca
      901 aaaagggaaa agagttagaa ttagaaacta ttttaattag tttgattcga cccacactcg
      961 atgaacctgg aaatattgct tatgttccac atagatcttt ggaaaatcct ctgtttttca
     1021 tgtttgatga gctatgggtt gatgaaaaag caatagaaga gcatttcatg caacctcaca
     1081 tgcataattt aatagaaaaa ttaaaacccc tgttagaaaa accgcttgag ttgaaaagat
     1141 attatgaaat aattttgtaa tcaatgacgc aaaaaaccac gaataaaaaa atattatgct
     1201 ttaaaattag tgagaacttt tgttataact ttatagagta cttatcacag agttttttat
     1261 aatggcttag ttttaattta tcttaatatc taaaagaaaa gatataataa aatcacttta
     1321 tgttggcaat agtttaagaa ctatctttgg aattataatt aagatagtaa acttgaattg
     1381 taatctaggt ttgtattatt tccatataat ataactgatg caaaaaaata atacgtaact
     1441 aaacagaccg gagtaataat ctaataagat tcaacaatag actttaaaaa tcgaaatctt
     1501 ttataatcat caaccagtat tttagataaa tgcttagtta tatctgttaa acttagaata
     1561 ctcacaattt gttgatcttg ctctaataca gg
//
LOCUS       DATAFW010000134         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3253072, whole genome shotgun sequence.
ACCESSION   DATAFW010000134 DATAFW010000000
VERSION     DATAFW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3253072"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /gene="spt4"
                     /locus_tag="VHJ57_03115"
     CDS             complement(<1..153)
                     /gene="spt4"
                     /locus_tag="VHJ57_03115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF041664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HEX2487554.1"
                     /translation="MPRELACKKCKAITVGKVCPICNSTDLSSDWSGIVIVFDSKTSL
                     IAKTLEI"
     gene            complement(156..746)
                     /locus_tag="VHJ57_03120"
     CDS             complement(156..746)
                     /locus_tag="VHJ57_03120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HEX2487555.1"
                     /translation="MFYIVSMTDIVRIPPSRLTNSLRNTAIGILKEKYESMISAELGY
                     VIMIIDANADSVGKLVTGDGATYHKVTFKALTFFPKIQEIVEGEIIEITDFGAFVRIG
                     PTDALLHLSQITDDYLKSDIKQGLIIANQSSRSLRIGSKMRARITAISLGKGSSMGKI
                     GITCRQPFLGSFDWIKEEIEKAKTPQAPKEKKGATK"
     gene            899..2020
                     /locus_tag="VHJ57_03125"
     CDS             899..2020
                     /locus_tag="VHJ57_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX2487556.1"
                     /translation="MVTVDQVYTSLKKVIDPELHKDIVSMGMIKEMTINNDKITFTLE
                     LTTPACPFNSEIEQDVRNAISDLGVKDLDLKVTARVMEGRAISLDELLPGVKNIIAVA
                     SGKGGVGKTTVSVNLAISLAKTGAKVGLLDADIYGPSVPLMLGLKESPQVVDGKIQPP
                     ISEGVKIISMGFFYEQSQQAGIYRGPIISGIIKQFLTDVNWGEIDYLIIDLPPGTGDA
                     PLTIAQTIPITGILVVTTPQEVAMNVAVKAIGMFNKLNVPIIGVIENMSYLQCPHCNS
                     NIHIFGKGAGQKISDQFKIPYIGDIPLHPQIMQGSDIGKSVILSDPESSQTKSFLKIS
                     KLVAGTVSVLAAEMQDQEKYEEKQDNSENPNLKQAKNAS"
     gene            2010..2540
                     /locus_tag="VHJ57_03130"
     CDS             2010..2540
                     /locus_tag="VHJ57_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HEX2487557.1"
                     /translation="MPLKDEHVNILKKPFGELIKQEDTSKQKINSLVKDSKSLITVGD
                     ATTEKIISLGLTPDLSIIDCVERRIRRTESKIFELKNFFLKNGQFKQYQCKNPKGTIA
                     KEAYITIKKILMKGEHAIIFIDGEEDMLALAVFALAPLDSIVFYGQPLEGLVSVKINS
                     KIKNKSRNLLDSIGVE"
     gene            complement(2650..2787)
                     /locus_tag="VHJ57_03135"
     CDS             complement(2650..2787)
                     /locus_tag="VHJ57_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487558.1"
                     /translation="MQPFRNVCSFCDSPISLGQSVCKKCINLYDIKTGKHENDGCGCD
                     S"
     gene            complement(3065..3277)
                     /locus_tag="VHJ57_03140"
     CDS             complement(3065..3277)
                     /locus_tag="VHJ57_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487559.1"
                     /translation="MPLDLIELAKSKLVYEYGVLADDIVLIDVPEGVPIGAIMVTLWP
                     HYLSVSKVKRVRDGSIYAPQLFNIEL"
BASE COUNT         1268 a          589 c          589 g         1155 t
ORIGIN      
        1 tatttctaga gttttagcaa taagagatgt tttggaatca aaaacaatta ctattcctga
       61 ccagtcacta gaaagatcgg tagaattaca aatcggacaa acctttccca ctgtaatagc
      121 tttacatttt ttacaagcta attctcgagg catttttatt tagttgctcc tttcttttct
      181 tttggagctt gaggtgtttt agctttttct atttcttctt taatccaatc aaatgaacct
      241 aaaaatggct gcctacatgt aattcctatt ttccccatcg aagatccttt acctaggctt
      301 atcgctgtta tccttgcacg cattttcgaa ccaattctca aagaacgaga agattggttg
      361 gcaattatca aaccttgttt gatatcactt tttagataat catcagttat ttgagagaga
      421 tgtaaaagag catcagttgg tcctattctt acaaaggcac caaaatcagt tatttctatt
      481 atttctccct caactatttc ttgaatttta ggaaaaaaag tcagtgcttt gaatgttacc
      541 ttgtgataag tggcaccgtc tccagtcact aatttaccga cggagtcagc attcgcatca
      601 ataatcatga ttacataacc caactctgca ctgatcatac tttcgtattt ttcttttaaa
      661 attccaatag cagtatttcg taatgaattt gtaagtcggc ttggtggaat tctaacaata
      721 tcagtcatag aaactatata aaacatgttg gacgagatag gatataatat atcaatttat
      781 gaaccttttg tttatatttt agaaaagaat aaaaatacag attggttttt acagtgaata
      841 ctatatcaaa tgaattgaaa tcaatattta aatattgcga aagagctttg tgtttataat
      901 ggttacagtt gatcaggttt atacttcgtt aaaaaaagta atagatcctg aattacataa
      961 agacattgtc tctatgggta tgataaaaga aatgactatt aataatgata aaataacatt
     1021 cacgttagaa cttactactc ctgcatgtcc tttcaatagt gaaatcgaac aagatgtaag
     1081 aaatgcgatt tcagatttag gtgttaaaga tttagatcta aaggttacgg caagggtgat
     1141 ggaagggaga gctatatctt tagatgaact tttacctggg gtaaaaaata tcattgcagt
     1201 tgctagtggc aaaggaggtg taggcaaaac aacagtctct gtcaacctgg caatatctct
     1261 tgcaaaaacc ggtgcaaaag taggtctact agacgctgat atttatggtc caagtgtccc
     1321 attaatgctt ggattaaaag aatctcctca ggtagtggat ggtaaaattc aaccgcctat
     1381 ttcagaaggg gttaaaatta tttcaatggg ttttttttat gaacaatccc aacaagcagg
     1441 aatttatcgt gggcccataa tttccggaat tatcaaacaa tttctcactg atgtaaattg
     1501 gggtgaaatt gattatctta ttatcgattt gcctcccgga actggagatg cgccattaac
     1561 aattgctcag actattccaa ttactgggat attggtcgtt acaacaccac aagaggttgc
     1621 aatgaatgtg gcagtcaaag caataggtat gtttaacaag ctaaatgttc ctatcatagg
     1681 ggtcatagaa aacatgagct atctccaatg tccacattgc aattcaaata tacacatttt
     1741 tggaaaaggt gcaggccaaa aaataagtga ccagtttaag attccatata ttggagacat
     1801 ccctttacat ccacaaatca tgcagggaag tgatataggc aaatctgtta ttctctccga
     1861 tcctgaatca agccaaacaa aatcattctt aaaaatttca aaattagttg ctggtacggt
     1921 aagtgtttta gctgcagaaa tgcaagatca agaaaaatat gaggaaaaac aagacaattc
     1981 ggaaaaccct aacttaaagc aagcaaaaaa tgcctcttaa agacgagcat gtaaatattc
     2041 taaaaaaacc ttttggtgaa ttaataaaac aagaagatac atctaaacaa aaaattaatt
     2101 ctctggttaa ggattctaaa tctttgataa cagttggcga tgctacaaca gaaaaaatta
     2161 tttcattggg tctcaccccg gatttatcca ttatagattg tgttgaaaga cgtattagaa
     2221 gaacagaatc gaaaattttc gaactcaaaa acttcttttt aaaaaatggg caattcaagc
     2281 aataccaatg taaaaaccca aaggggacaa tagcaaaaga ggcatatatt acaataaaaa
     2341 aaatattaat gaaaggagaa cacgctatta tttttattga tggtgaggaa gatatgctag
     2401 ccttagcagt ttttgctctt gctcctctag attctatagt tttttatggt caacccctag
     2461 agggattagt tagcgtaaaa ataaattcta aaataaaaaa taaatcaaga aatttgctag
     2521 atagtatagg ggtagaataa attccctggg gatgatgaag tggtggctgt atgaagaaat
     2581 atctaatgat ttgcgatacc attttctgac cctgatatta aattgaagaa taataaaaat
     2641 tgctactttt caggagtcac atccacatcc atcattctca tgtttaccgg ttttaatatc
     2701 atacaagtta atacacttct tacatacact ttgacccaaa gaaattggag aatcacaaaa
     2761 tgaacaaaca ttcctaaacg gttgcattat tagaatgtgt catgaatttc catatagttt
     2821 ttactggaaa attaacatta gaaaaatgaa aatctataat tattccttaa ttatgacatg
     2881 aacattcaca ttttgttagt tttatgtcaa aagagcagtc aaagtttgga ccacaaacat
     2941 cactaatagg aagctcccat ggacaatcgg aatgtcggtc acgtttacag aaaaaagaaa
     3001 tcctcacatt tttaactaat taaagggcaa attaattttt aactataaat ataagaatta
     3061 ctagtcagag ttctatatta aataattgag gagcataaat tgaaccgtct cttactcttt
     3121 ttacttttga aaccgaaaga taatgtggcc aaagagtaac catgatggca cctattggga
     3181 caccttctgg gacgtcaatt aaaacaatat catcagccaa aactccgtat tcatagacta
     3241 atttggattt agctagttca attagatcaa gcggcattcc actactatat aaataaacct
     3301 gacctgagta ttcgattttc gtattcatat atttaattat tagataaaag tattaataaa
     3361 tagttgtaac gtgaatattc cgtatttata ttgtaaatta aaatatatta aaaattcttg
     3421 tattaagcta catattcaga gttcatcaat atgatattta gacatacaac ctttacattc
     3481 atatatttca tcacccacag gaccagagac attgaatctc actatggtag tacattttgg
     3541 acatcgacct tcaacacttt tagtctttct ttgaggttta attgtgcgtc tttttctact
     3601 t
//
LOCUS       DATAFW010000135         7643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_611612, whole genome shotgun sequence.
ACCESSION   DATAFW010000135 DATAFW010000000
VERSION     DATAFW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611612"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VHJ57_03145"
     CDS             complement(<1..162)
                     /locus_tag="VHJ57_03145"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX2487560.1"
                     /translation="MGTQMTSARRGIPTEEMLQVAKDEDIDINFIIQKVANGSIIIPR
                     NNKRKQKIKV"
     gene            332..1273
                     /locus_tag="VHJ57_03150"
     CDS             332..1273
                     /locus_tag="VHJ57_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX2487561.1"
                     /translation="MNFFIHDDNFYNKFPHSYHKEPFLLEKLLFRLSKKWIAGYSMDD
                     AINYALNAKERGLNTIINYLGEDLKNKEIVKKTIIEYKNLVKKMKERGIAGSISIKPT
                     QIGLSLNMDFCLDKLLEIISVAKKNNIFIWIDMESFKNIDSTLTIYKKAREETNEIGL
                     VLQSYLKRSGSDLIKLLAYKPNIRLVKGAYHEKEEYAFKTKFDIDNNYVEMAKLLFAQ
                     KRNLRENQIFAIATHDANIIQKCLDLFYLYNFKEEHLQFQFLMGIREQIKINLLSRGF
                     NVHEYVPYGENWLPYSIRRLRERKSNIILLLRSLISP"
     gene            complement(1287..1676)
                     /locus_tag="VHJ57_03155"
     CDS             complement(1287..1676)
                     /locus_tag="VHJ57_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487562.1"
                     /translation="MGRWDNDSGRKSPLQLNKKKIKYIMLGGFVIVAIVVLSVFIPRA
                     GLTVEILERSDSMHSVKTISIKVNNNSLQEIKDVKVQFGENGKLHSYGNIGPFTAIFI
                     TPDPDELNFDRVIVSANAGNIKTIKFR"
     gene            1810..2322
                     /locus_tag="VHJ57_03160"
     CDS             1810..2322
                     /locus_tag="VHJ57_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2487563.1"
                     /translation="MNSSDDSNSTYIYEIMSKNVVKVNHEISALEISKIMVKRRISSV
                     VIIDNNDKIIGIVTEKDLIREICGKNLLANTLTAGKVMSSPLITISKNSTINDATKLM
                     VEKKIKHLAIHENNDIIGILTTYDLINVLRNKIKSMDLDSNLLDAISMADIPLEEGGF
                     SLPLSEDELK"
     gene            2442..2867
                     /locus_tag="VHJ57_03165"
     CDS             2442..2867
                     /locus_tag="VHJ57_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX2487564.1"
                     /translation="MDTDPHTDEEIKEFYKLKNIAVVGMSKNEEKPSHFVPKYLVKNG
                     YNIIPVNPTTEEILEIKSYKLVSEIKEDVDIVDVFRKSEDIIPVVDDLLKKKGIKVIW
                     LQKGIFNQHAGELAKKNGIDFVYNRCMLEEHQRLFSGKR"
     gene            3360..3551
                     /locus_tag="VHJ57_03170"
     CDS             3360..3551
                     /locus_tag="VHJ57_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487565.1"
                     /translation="MILSLIVLGVIILILGIIFYFQSKSFVGPKRSFMYNNPNWTING
                     FILILFGVSLVVVGFFTAL"
     gene            3662..4213
                     /locus_tag="VHJ57_03175"
     CDS             3662..4213
                     /locus_tag="VHJ57_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487566.1"
                     /translation="MKPHSVSYLVRNYEKYLFTILLLAIVSVVGFTIEKAYPQQEDSQ
                     LNGPAMNSQKSDESTSFSDTKFTPKMFVKITSHKNADKVPAGELTINGTSSDTAARDC
                     VVEVDWNNEKPSQIVTPTGPGGSSDYSTWTFNYTKEYREIEVGPNELTSKLTCINPGP
                     LTKWFSVTLTGEQPPFPRPLPVP"
     gene            4288..5820
                     /locus_tag="VHJ57_03180"
     CDS             4288..5820
                     /locus_tag="VHJ57_03180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX2487567.1"
                     /translation="MNFKYFFTNSTSYTVSKQEIYIIFSIFVIALIIRSYDLGGLALN
                     NDEAIYAGQAASLANYSEYQDQFSIFRAHPLLLQFMISISFYFFGINEFTARIIPVFL
                     SCGIVVISYFIAKFLYNKQTAILSSIILSLLPYHVAISKQALVDVSMSFFFVLNLLLI
                     VLYLRQNTFVLLYGIGITSGLSFLSKEVGVISFVISIISIIIINKKIKVKNIVYITLS
                     FIFVTSPYWISFITLEEARESILEYIDWQSSRPPNHDNTYYFLIICTDILGYVLTPLL
                     VISYLYILFNKKTWKGSNLLLPFVWIAIPLLFYQLVSVKGYHFLFSITPLLVIFGISF
                     LSGDWIKKISSKKILLISIIPITLLSNNQTINEYFLFTSYTPVLGSESLFHMKDAALW
                     IKENTPENSTILTLYTHMSNIIKFYSLRDSVAIQSNNNPSYQKIENIDISILANNIDY
                     IVYEKIQINNGKFLQSKATELEEYVKKYDGAPVYVNYQNYSNVGNNKLLSSPAIIVYE
                     FN"
     gene            5884..>7643
                     /locus_tag="VHJ57_03185"
     CDS             5884..>7643
                     /locus_tag="VHJ57_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX2487568.1"
                     /translation="MLFKSLYFIFLIFFYFSLSSIVFVITAVYSQDNNGLSQKNKTPI
                     DHVIVISQGRRSFDNYFGSYEGVNGFQMNVGIPINPFQFSPSFQNFTISLSFNSNTSN
                     VGKESFLINKGGIGKESPGNNLNFGIWLNEKEHLTGGFETKEGKDFFVASKNKYRDNN
                     WHHVILTYNKSVLKLYVDKKLQAEKITEGEIPDDNNLPFIRLGANFLKQNNFYTGSID
                     NILVWNRSLNANEFSSFFTNKDIPAGSIVEVGINTTNMKYENKNAPLNFNGTNYFDLK
                     VIPDITEGLIEPFHLKNTKTPNLKYGPQVYDKSYNYGFMDGFIFAQNDNKVTPNDIPN
                     KYNHTIVMGYYNNSEIGPYWSLASNFVLADNFFSSTRQTDLTNNLHLFSAEEGNFKKY
                     VPKAGLSINSTVLDLLEKKRITWKIYVENYNSSLNYTNEDESSKRYLTLNPILGIPRF
                     VENSTLNGRIQDLSNYFHDIKNNFPSLSYIILPDSQESSPKDILKGQESVVSLIISLM
                     QSKHWKNSLFILTYSGSGGWYDHVPPAFNSEYERGFRVPTLFISPYSHEGVVDSTFYD
                     TLSILKFLEYNFNLKSLNIK"
BASE COUNT         2792 a         1055 c         1069 g         2727 t
ORIGIN      
        1 aacttttatc ttttgttttc ttttattatt tctgggaata attatagaac catttgctac
       61 tttttggata ataaaattta tatctatatc ttcatctttt gcaacctgga gcatttcctc
      121 tgtgggaatt cctcttcttg cgcttgtcat ctgagtaccc ataatcctat taaatataga
      181 acaaatgaat ataaaagaat gtctccactt gatctatcaa attttgaagt tcaacgaaag
      241 aattctattt aataattcat cacaaacctt gatgtactat aacatttgta ttaccaaaat
      301 acctttaata taaataaatg taatatagat tatgaatttt tttattcatg acgacaattt
      361 ctacaataaa tttcctcatt cttatcataa agaacctttt ctattagaaa aactcctttt
      421 taggttatct aaaaaatgga ttgctggtta tagtatggat gacgcgataa attatgcttt
      481 gaatgcaaaa gaaagaggac taaatactat tatcaattat cttggagaag atttaaaaaa
      541 taaagaaatt gtaaaaaaaa caataattga atataaaaat ctcgtaaaaa aaatgaaaga
      601 aagaggtatt gcaggatcaa tttcgataaa accaacacaa attgggttat ccttaaatat
      661 ggatttctgt ttagacaaat tactagaaat aatttcagtt gcaaaaaaga ataatatttt
      721 tatttggata gatatggagt ctttcaagaa cattgactcc actttgacaa tttataaaaa
      781 ggctagggaa gaaactaatg aaataggact tgtactccaa tcatatctca aaagaagtgg
      841 ttctgatttg ataaaattat tggcatataa gccaaatata agattagtaa agggtgctta
      901 tcatgaaaaa gaggaatatg cattcaaaac gaaatttgat atagataaca attatgtgga
      961 aatggcgaaa cttttatttg ctcaaaagag aaatcttaga gaaaaccaaa tttttgcaat
     1021 tgcaactcat gatgccaata taattcaaaa atgcttagat ctattttatt tatataactt
     1081 taaagaagaa catttacaat ttcaattttt aatgggaatt cgtgaacaaa taaaaatcaa
     1141 tttattatca agaggattta atgttcatga atacgtacca tatggtgaaa attggctccc
     1201 ttattccatt agaagactac gagaacgaaa aagtaatata attctattac tgcgttcatt
     1261 aatttcacct tgatatataa tatccattat ctgaatttta tagtcttaat atttccagca
     1321 tttgcagata caataactct atcaaagttc aattcgtcag gatccggagt tataaatatg
     1381 gcagtgaatg gtccaatatt tccataagaa tgcagtttac cattttcacc gaattgaacc
     1441 ttaacatctt tgatttcttg gagactatta ttgtttacct tgatactaat tgttttgact
     1501 gaatgcattg aatcgcttct ttcaagaatc tctacggtca atcctgctct cggaataaaa
     1561 actgataaaa caacaatcgc aacaattaca aacccaccta acataatata ttttattttt
     1621 ttcttgttta gttgtaaagg tgatttcctg ccagaatcat tgtcccatct tcccacattc
     1681 ttaaatagaa ttacatttta ataaagttca tgattaaatt ttaggcttca atctttatat
     1741 ttttcatgtc attactattt attttaaatt aaataaaatt taattaataa ccttttgaaa
     1801 tttatattga tgaatagttc agatgattct aactcaacat atatttatga aatcatgagt
     1861 aaaaatgtgg taaaggtaaa tcatgaaatc tccgcattag aaatatctaa aatcatggta
     1921 aaacggagga ttagttctgt agtcataata gataataatg ataaaattat aggaattgtt
     1981 acagagaaag atctaattag agaaatatgt ggaaaaaatc ttcttgcaaa tacattaaca
     2041 gcaggaaaag taatgtcatc tcctttaata acaatcagta aaaattctac cataaatgat
     2101 gcaacaaaat taatggtaga aaaaaaaata aaacatttag caattcatga aaataatgat
     2161 atcattggaa tacttactac atatgatctc atcaatgttt taagaaataa gattaaatct
     2221 atggatttag attccaatct attagatgcc atatcaatgg ctgatattcc tttagaagag
     2281 ggtggatttt ctcttccgtt atccgaagat gaattaaaat aaataaaaaa atttgatgat
     2341 tatatttcct ttatatgttt ccaaattata tttataatta ttgataacgt tataccgtta
     2401 gtttaataat caataaatac tttgagatat attttgaaat aatggataca gatcctcaca
     2461 cagacgaaga aataaaagaa ttctataaat taaaaaatat tgcagtagtt ggtatgtcta
     2521 aaaatgaaga aaaaccttct cattttgtac caaaatattt agtgaagaat ggttataata
     2581 taataccggt aaatcctaca actgaagaaa ttttggaaat aaaatcctac aaacttgttt
     2641 cggaaatcaa agaagatgtt gatattgtcg atgtatttcg aaaatcagag gatattatcc
     2701 ctgtagtaga tgatttatta aagaaaaagg gaataaaggt aatctggtta caaaaaggta
     2761 tttttaatca acatgcagga gaattggcaa aaaaaaatgg aatcgatttt gtatataata
     2821 gatgtatgct agaagaacat caaagactgt tttcgggtaa aaggtgaaat ctaaaataac
     2881 ctatgattcc tataagtaca agaacatagt gtttatatat tttaagtcgt cagtttttaa
     2941 cattgcacat tctgtttaaa taaataggct acatttcatg cattatgtag atatttattg
     3001 gaactcaaat taggtattag tgacttaatt tctctattag ttcattacaa atattataag
     3061 tttataaact catttcaaaa aacccgttca caaatgtctt attgaaaaat tatgaatgat
     3121 tgttaatgaa atattataaa aaattcaatg cgattattaa tagaatattt aaggatttgt
     3181 caagaatgaa ataaaactaa tattcttcct aaaatctatg tattactgag gatagattct
     3241 aactcatttc actttatcta taccttatcc taaaaaatga taatttaaat gtgaagttaa
     3301 atattataca aagggaaaag gattattatt ctcattatgc tttttatttg aaatagatca
     3361 tgatattatc attaattgtt ttaggagtaa ttattctcat tcttggaata atattttact
     3421 ttcaaagcaa atcctttgta ggacctaaac gctcttttat gtataacaat ccaaattgga
     3481 ctatcaatgg atttatcttg attcttttcg gagtttcttt agtagtagtc ggttttttta
     3541 ccgcattatg atgaaacaac atctttaaaa aataagattt caaaaagcga atatatgttt
     3601 agtagctgct ctatccattt ttacactact ttttaaataa atattcttat tttcaagttt
     3661 aatgaaaccg cattctgttt cttatttagt gagaaattat gaaaaatatc tctttacaat
     3721 tttactatta gcaatagttt ctgtagtagg atttactata gaaaaagcat atcctcaaca
     3781 ggaagattca caattgaatg gacctgcaat gaattctcaa aaatccgatg aatcgacatc
     3841 attttctgat actaaattta ctcctaaaat gtttgtaaaa attacatctc ataaaaatgc
     3901 tgacaaagtt cctgctggag aacttactat caatggaacg tcaagtgata ctgctgctag
     3961 agattgcgta gtagaagtag attggaataa tgaaaagcca tctcaaatag ttactcctac
     4021 aggaccaggc gggtcgagtg attactcaac atggacattt aattatacta aagaatatcg
     4081 tgagatagaa gtaggaccta atgagttaac atcaaaatta acctgtataa atcctggacc
     4141 tctaacaaaa tggttcagtg ttacattaac tggtgaacag ccaccatttc caagaccgtt
     4201 acctgttcct taatatttat tttctattag tattacctat aaaagataaa aaatataaat
     4261 catttttgct gctctacagc atatctattg aattttaaat atttctttac aaatagtact
     4321 agttatactg tatcaaaaca agaaatatat ataattttct ctatctttgt aattgcatta
     4381 attattagat cttatgatct gggtggattg gctctcaata atgatgaggc aatatacgct
     4441 ggacaggcag catcacttgc aaattatagt gaatatcaag atcaattttc tatctttaga
     4501 gctcatccgt tactattgca atttatgatt tctatctcat tttatttttt tggtataaat
     4561 gagtttactg ctcgaatcat tcctgtgttt ttgagttgtg gtattgttgt tatatcttac
     4621 tttattgcta aatttctata caataaacaa actgctattc tatcttctat tattcttagt
     4681 ttgcttccat atcatgtagc aatctccaaa caggcacttg ttgatgtttc aatgtcattt
     4741 ttctttgttc ttaatcttct actaattgta ctatatcttc gccaaaatac atttgtactt
     4801 ctatatggaa taggaatcac gtcgggactt agctttcttt caaaggaggt tggagtaatt
     4861 tcttttgtta tttctattat aagtatcatt attataaata agaaaattaa agttaaaaat
     4921 atagtatata ttactttatc ttttattttt gtaacttctc catattggat ttcttttatt
     4981 actttagaag aagcacgtga atcaatatta gaatatatag attggcaaag ttctagacct
     5041 ccaaatcatg ataatacgta ttattttctt ataatctgta ctgacatttt agggtatgtt
     5101 ctaactccct tgctcgtcat ttcctattta tatatcctat ttaacaaaaa aacctggaaa
     5161 ggatcaaatt tgttgcttcc ttttgtttgg attgctattc cactactttt ttatcaatta
     5221 gtgtccgtta aaggatacca ttttctcttt tcgattacac cattgcttgt tatctttgga
     5281 atttcttttc tttctggaga ttggataaag aagatttcaa gcaagaagat tttgttaatt
     5341 agtataattc cgataacatt actttcaaat aaccaaacca tcaatgaata cttcttgttt
     5401 acttcgtata ctcctgttct gggttcggaa tccctctttc atatgaaaga tgcagctcta
     5461 tggattaaag aaaatacgcc agagaattct acaattctta ctctgtatac tcacatgtct
     5521 aatataataa aattctattc cctaagagat tcagtagcta ttcaatcaaa caacaatcca
     5581 tcttaccaaa aaatagaaaa tatagatatt tcgatcttgg ctaataacat agattatatt
     5641 gtttatgaaa aaattcaaat taataatgga aaatttctac aaagtaaagc aactgaacta
     5701 gaggaatacg tcaaaaagta cgatggagct ccagtttatg ttaattacca aaattacagt
     5761 aacgttggaa ataataaatt attatcaagt cctgcaataa tagtatatga atttaactaa
     5821 tgatatgatt ttcacctagg ttttataaga catttagttt atgataataa ccttgatgta
     5881 aaaatgctat tcaaatccct ttatttcata tttttaattt ttttctactt ctcacttagt
     5941 agtattgttt ttgttattac agcagtctat tctcaggata ataacgggtt atctcaaaag
     6001 aataaaacac cgattgatca tgtaatcgtt atttctcaag gacggagaag ttttgataat
     6061 tattttggaa gttatgaagg agttaatgga tttcagatga atgtaggaat tccaataaat
     6121 ccgtttcaat tctcaccatc ctttcaaaat tttacaatat ctttgagttt taatagtaat
     6181 acttctaatg tagggaaaga aagttttctt ataaacaaag gtggaattgg gaaagagtcg
     6241 cctgggaata atttaaactt tggtatatgg cttaatgaaa aagagcatct aacaggcgga
     6301 ttcgaaacaa aggaaggaaa agattttttt gtagcatcaa aaaataaata tagagataat
     6361 aattggcatc atgttatatt aacatacaac aaatctgtct taaaattata tgtagataaa
     6421 aaattacaag cagagaaaat tactgaagga gaaatacccg atgacaataa tttgcccttt
     6481 attagattag gagcaaattt tttaaaacaa aataattttt atactggtag tatagataac
     6541 attttagttt ggaatcgatc tttgaatgca aatgaatttt cttcattttt tacaaataag
     6601 gatattccag ctggatcaat tgtagaggtt ggaataaata ctactaatat gaaatatgag
     6661 aataaaaatg ctcctttgaa tttcaatggg actaattatt ttgatttaaa agttatacct
     6721 gatattactg aaggtttgat agaacctttt catttaaaaa atacaaaaac accgaattta
     6781 aaatatgggc cacaagtata tgataaatct tacaattatg gttttatgga tgggtttatt
     6841 tttgctcaaa atgataacaa ggtaacacca aatgatattc caaataaata caatcataca
     6901 attgtaatgg gatattataa caatagtgaa attggtccat attggtctct tgcttcaaac
     6961 tttgttctag cagataactt ttttagttcc actagacaga ctgatttaac taataattta
     7021 catttattta gtgcagaaga agggaatttt aaaaaatatg tacctaaagc aggtttatcc
     7081 attaattcaa cagtattaga cttattagaa aaaaaaagaa ttacgtggaa aatttatgtc
     7141 gaaaactata attctagtct aaattatacc aatgaggatg aatcatccaa gcgttatctt
     7201 actttaaatc ccatccttgg gattcctaga tttgttgaaa atagtacact caacgggaga
     7261 atacaagacc tgagtaatta tttccacgat ataaagaata atttcccctc tttatcgtat
     7321 attattttac ctgatagtca agaaagtagt ccaaaagata ttttaaaagg acaagaatca
     7381 gttgtctctt taatcatttc tttaatgcaa agtaaacatt ggaagaatag tctttttatc
     7441 ttaacttata gtggctcagg aggttggtac gaccatgtgc cgcctgcgtt caattctgaa
     7501 tacgaacgtg gttttagagt accaacacta ttcatttctc catattccca tgagggtgta
     7561 gtagattcta ctttttatga tacattgtcg attctaaaat ttctagaata taattttaat
     7621 ctaaaatcat taaatattaa aga
//
LOCUS       DATAFW010000136         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4841838, whole genome shotgun sequence.
ACCESSION   DATAFW010000136 DATAFW010000000
VERSION     DATAFW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4841838"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VHJ57_03190"
     CDS             <1..389
                     /locus_tag="VHJ57_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019712.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2487569.1"
                     /translation="TSVPTLGICLGNQILALSAGADTYKLKYGHRGQNKSCINKENSL
                     SYITSQNHGYGIDPGTLTSTDFRPWFYNPDDNTIEGIIHNNKPILAVQFHPEASPGPN
                     DCNYVFDKFKEIVNLNKREKLSNKET"
     gene            571..3452
                     /gene="carB"
                     /locus_tag="VHJ57_03195"
                     /pseudo
     CDS             571..3452
                     /gene="carB"
                     /locus_tag="VHJ57_03195"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
BASE COUNT         1299 a          461 c          672 g         1020 t
ORIGIN      
        1 atacatctgt acccactctg ggaatatgtt taggaaatca aatacttgca ttgtctgctg
       61 gtgcagatac ctataaacta aaatatggac atagaggaca aaataaatca tgtattaata
      121 aagaaaatag tttatcttat ataacaagtc agaatcatgg atatgggata gatccaggaa
      181 ctcttacttc tacagatttt cgaccgtggt tttataatcc agacgataat acaatagaag
      241 gtataattca taataacaaa ccaattcttg ctgtccaatt tcatcctgaa gcatctccag
      301 ggcctaatga ttgtaattat gtatttgata aatttaaaga aattgttaat ttaaataaga
      361 gagaaaaatt atctaataaa gaaacttaaa tatcaaaggt aaggggtgaa aaaaattcca
      421 agaagagaaa ctataaaaaa agttgtggta ttgggtagtg gtgcaatcaa aatcggagaa
      481 gcaggcgaaa gttaggattc tagccgctaa tttgactact ctggttcaca atgtctaaaa
      541 gctctaaaag aagatgaggt aaagaccgtt ctaattaatc ctaatattgc tactattcaa
      601 acagatacta gattttctga tgtcgtatat ctattgccta ttactaaaga atatgtatcc
      661 aaaatattgg aaatagaaag acctgattct ataatgttat catttggggg acaaactgca
      721 cttaattgtg gaatggcttt attcaaagaa gggatactaa ataaatatgg gactcaagtt
      781 ctaggtacac ctatcgaagg tatagagaga acagaaaata gacagctctt taaagaagca
      841 atgcagaaat gtaatgttcc tgtattatct agcgcaccag cctattctgt agaagaagca
      901 ataagagttg ccaaaaatat tggttatcca gttattgtaa gagttgctta tacattggga
      961 ggaaaaggag gaggagtagc acacaatgaa tacgagcttc aagaaattgt acatcgcggt
     1021 attgctctaa gtatgagtga acaagtactt attgaaaaat atgttggtca ttggaaacaa
     1081 atagaatatg aggttatgcg agatcacaaa ggaaataata taatagtttg taatatggaa
     1141 aatgtacttg gtatgaaagt gcatactggt gataatatag tagtagctcc gtcacagaca
     1201 cttaataatt atgaatatca tatgctccgt tcagcggcta tgtgggcgac aaatgaatgt
     1261 ggtataatag gtgaatgtaa tattcaattt ggtctggaac cttcttctga aaaatattgc
     1321 gcaatagaaa tcaatgctag actatcaaga tcatctgctt tagccagtaa agcaactggt
     1381 tatcctttag cttacatggc tgccaaattg ggacttggtt ataccttaga tgaattaata
     1441 aacaaagtga caaaaaaaac tacagctttg tttgaaccat ctttagatta tattgtagtg
     1501 aaaatgccac gatgggactt taataaattt gaaaaagtga atactagact tggaactact
     1561 atgaaatctg tcggtgaagt aatggctata ggacgaacat ttgaggaggc aatacaaaaa
     1621 gcagttagaa tgtgcgatat tgggaaaatt ggaattgtag gcaataatag atcagaagaa
     1681 aaagtgatac tagaattaga aaatatagaa tattctcttt taaatcctaa tgacaacatt
     1741 ttgacggatg tagttaaagc aattaatttt ggaatgacaa ttgaaagaat aaatgaatta
     1801 tctagtatcg atccatggtt tttaaacaaa ataaaaaata tcatagaaat ggaaaaaaaa
     1861 ttaaaaaatt cgtcaaataa ttttcataaa aagatgataa gagaagcaaa aagcttggga
     1921 ttttccgata aacaattagc ttattatttc aatctagatg aatatcaggt gaggcatatt
     1981 aggaagacat ttggaattga accagtaata aaacaaattg atacccttgc tgctgaatgg
     2041 ccagcacaga caaattatct atatcttaca tatggtggac agtacgacga tgtcaaaatt
     2101 aagaacaaag gtaaaaatgc aatagtattg ggtgcaggtc catatcgaat tggaagtagt
     2161 gttgaatttg attggggaac agtaaatatg gttatgggtt taaaaaataa tgacatcaac
     2221 tctgtctcag taataaattg taatccagaa accgtttcga cagattttga tatttcagat
     2281 cgtttatact ttgaagagat tacattagaa agggttctgg atatctatga aaaagagaaa
     2341 tcagatggta taataacatg tgtaggtggt caaactgcta acaatttgac acagaatttg
     2401 gcaaagaatg gtgcaaagat aataggaacg tctagttatg atgttgatag agcagaagat
     2461 cgagaaaagt tttccaaact tttagattct ttaaatattg ctcaacctcc ctggaaaaaa
     2521 ttttcgaatc ttaatgaggc aaagatgttt tctgaatcag tacagtatcc agtattggta
     2581 agaccttctt atgttctgag tggggcggct atgaaagttg tgtgggatga taagcagtta
     2641 gaaaaattta tagagaatgc aactgtggta agtccagatc atccaatagt aatcagtaaa
     2701 tttttccaag atgcatctga agtggaagta gatggtgtag ggtccaaaga taaggtaatt
     2761 attggcgcta taatagaaca tatagataat gctggtattc attcaggaga tgcaatgatg
     2821 tctttacctc catggggatt aacaagaaat ataatggata ctataacaga ttattctaat
     2881 aagatcgtaa gagcattgaa tgtatcagga ccttttaata ttcaattcat agttaaaaat
     2941 gaggaagtgt tggtgataga agccaatata agagcatcga gatctatgcc ttttgtttca
     3001 aaatttgtac gcataaatct tatcgactta tccgcaagag caatgttgaa taaaccttta
     3061 cctaatatcc atgaagaact atggctgaaa acaactggat tcgctataaa agttcctcaa
     3121 ttttcattca tgcaattaga aggtgcggat atagttcttg gtgttgaaat gcattctacg
     3181 ggggaagtag cttgttttgg aactagtttt tacgacgcac taagtaaatc ttacatagcc
     3241 gcaggttatt ctttaccaaa aaaaggaact tcattaatta caataggagg acagataaat
     3301 aaggaaaaat tactaccagt tatatctctc atgacatcaa tggattataa aatcatggct
     3361 accgaacata ctgctgattt cttagaaatt aatgggatta aagatattca aagagtttat
     3421 aaaatcaatg aacctgaaag aaaaccaaat at
//
LOCUS       DATAFW010000137         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_789968, whole genome shotgun sequence.
ACCESSION   DATAFW010000137 DATAFW010000000
VERSION     DATAFW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789968"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..267)
                     /locus_tag="VHJ57_03200"
     CDS             complement(16..267)
                     /locus_tag="VHJ57_03200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF007129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HEX2487570.1"
                     /translation="MSVDKKIVKVKNHIYQPRHEILTKDESEEILKKFNVRTNQLPYI
                     MSSDKGLEDLDIRPGDIIKITRKSPTAGESVYYRYVVQG"
     gene            complement(419..519)
                     /locus_tag="VHJ57_03205"
     tRNA            complement(join(419..453,481..519))
                     /locus_tag="VHJ57_03205"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:His,seq:gtg)
     gene            complement(577..1395)
                     /locus_tag="VHJ57_03210"
     CDS             complement(577..1395)
                     /locus_tag="VHJ57_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX2487571.1"
                     /translation="MDTGELMKIGLDLAGWKKIPYDSAIHVKGNNIKKVFVSIDVSTF
                     DIYFAKNLGCDCVLTHHPIGRSAISFHKVFDRHVEYMVEKGIKKNLAISLVKDLKYKA
                     YLKSHSLIYNDVISIAKRLNMPLLNIHQPLDEYMRKVILFKLYNSKLKYVSDLMKAIK
                     EITEFKKALTRVIICNGKSSNEIGEWVLVIAAGSNGGYDIARTYYENNVSTVIYLHID
                     YNELLKVKKDKRCKNLVILGHLAGDSLGMNKMCEELKKREIVVVKRGLIDDREW"
     gene            1567..>1810
                     /locus_tag="VHJ57_03215"
     CDS             1567..>1810
                     /locus_tag="VHJ57_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487572.1"
                     /translation="MFKKFINFYLRYQNISLKISFILISLQLLHLYWLTTDVVIYRLT
                     GIDYFVGLSDFILLFIIIDYIEIPALVSGIIYYFFAI"
BASE COUNT          581 a          296 c          216 g          717 t
ORIGIN      
        1 ataacttgta tctccttagc cttgtactac atatctgtaa tatacacttt cacccgcggt
       61 tggacttttt cttgtaattt taattatatc tcctggtcga atatccaagt cttctaatcc
      121 tttatcgctt gacataatat aggggagttg attcgttctt acgttaaatt ttttaaggat
      181 ttcttcagat tcgtcctttg taagaatttc gtgtctaggt tgataaatgt gattctttac
      241 tttgacaatt tttttatcta ctgacagatt tacaaacctc ctctaaattt atcagtagat
      301 aaataattct ggataaaata gcttctcgca atacgaaatc agtaacattc atggagtaag
      361 aggtatatgt agtttcttac aatattttgg accaataact tagttttctt tatatttagg
      421 ccttcggagg gatttgaacc ctcctcaagc ggtttctatc ttcttattta taataaaaga
      481 ccacagccgc ctatactaac caggctatac tacgaaggcc atgaataatt tatgaaattc
      541 tgtaatattt attctttaga gggattaaat tgccaatcac cattcacgat catcaattaa
      601 accacgttta acaactacta tttctctttt ttttagttct tcacacattt tgttcattcc
      661 taatgaatcc cctgccaaat gtccaaggat tacaagattt ttacatcttt tatctttttt
      721 tacttttaaa agttcattat aatcaatatg gagatatatt actgtggaaa cattattttc
      781 ataatatgtg cgtgcaatat catatccgcc atttgatcca gctgctataa caaggaccca
      841 ctcacctatt tcattacttg actttccatt gcaaattata actcttgtta aagctttttt
      901 gaattctgtt atctctttga ttgctttcat taaatccgag acatatttta atttagagtt
      961 ataaagtttg aataaaataa ctttcctcat atattcatca agaggttgat gtatattcaa
     1021 taatggcata ttgagtcttt ttgcaatgct aatcacatca ttgtaaatta aagaatgaga
     1081 ttttaaatag gccttatatt ttaaatcttt aaccaatgaa attgctaaat tttttttgat
     1141 acctttttcc accatatatt ctacatgtct atcaaaaact ttatgaaatg aaatcgctga
     1201 tcgtccgatg ggatgatgtg ttaagacaca atcacaacca agatttttag cgaaataaat
     1261 atcaaaggta ctcacatcaa tggaaacaaa aacctttttt atattatttc cctttacatg
     1321 aattgcgcta tcatatggta tttttttcca tccagctaaa tctagtccaa ttttcattaa
     1381 ttcacctgta tccaatcctt tcaatactat atgattatca taattgtcga atatttactt
     1441 ttaagtgaaa ctctccattt tttctaaatt taatattaat aatggttatt ttatgaaatc
     1501 ttaactagaa tacgacttta taggtaaaaa ataataataa tagaaactat gaaaaattga
     1561 tttatagtgt ttaaaaaatt tattaatttt tacctccgat accaaaatat tagtttaaaa
     1621 atctcattca tattaatctc attacaatta ttacatcttt attggcttac tacagatgtt
     1681 gtaatatatc gtcttacagg aatagattat tttgtaggac tatctgattt tattttgtta
     1741 tttataataa tcgactatat agaaattcca gctttagttt caggtataat atattacttt
     1801 tttgctataa
//
LOCUS       DATAFW010000138         4592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_614057, whole genome shotgun sequence.
ACCESSION   DATAFW010000138 DATAFW010000000
VERSION     DATAFW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614057"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VHJ57_03220"
     CDS             complement(<1..399)
                     /locus_tag="VHJ57_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487573.1"
                     /translation="MNDRWILEKQHKALLFFASIAVKSFLMAITTSYIFCKSIDPSIL
                     FLNCSMNIVYVGISIGIIIVMGIVFGYYIIRIKSFDDIFANMMKSDYILTTMYFSMAF
                     LAPALMSTMIIIWLFGVWILGMLLAPFNKEK"
     gene            722..1987
                     /locus_tag="VHJ57_03225"
     CDS             722..1987
                     /locus_tag="VHJ57_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX2487574.1"
                     /translation="MKILFKWISIDGKLLLCARIVRTFAYGFLSVILAIYLKLIGFDD
                     ILIGIILSITLINSIIFTLFASFYADRIGRRNTLLLYTIMMSISGGIFFVTENTLALI
                     IAALIGTLNITGSETSAFLSVEQSILPQTIKDNRKRNTLFGFYNMAGTFAMGAGILIA
                     NLPIIIQNELEFDQIYAIKLLFLFYSLLGLSIIGIYLKLSSAIEIKEKEKTLKPLSKT
                     LNPKSKKIVAKLSGLFAIDSFAGGFAIQTIVSFWFFSRFDIDLSTISYIFSIGSVLTA
                     FSYLIAAKIADKIGLINTMVFTHIPSNILLILLAFAPNLEIAIVFYMIRMSLSQMDVP
                     TRQSYIVSIVEEEERTASAGITNLSRNIAQAISPSITGYIIGFLSLSAPFIIGGLLKI
                     LYDIVLYRNFRKIKPSEEEEEEKEEEEER"
     gene            2689..4368
                     /locus_tag="VHJ57_03230"
     CDS             2689..4368
                     /locus_tag="VHJ57_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX2487575.1"
                     /translation="MSTNYSYKQKENYDDEFEKKTSGITESPWYDEIQQQQPMKFDKL
                     RRNISHSESVDVSIVGGGIVGLSTAYLLSKSGKKVVVFEDGYLCSGETGRTTAHITHA
                     LDDRYYNLEKLHGMEGTRLVAESHTSAINMIEAIVNQEHIDCDFERLDGFLFLDPTDT
                     LESLEKELEATHRAGITDTEIISKVSGLSFDTGPCLLFPNQAQFQPLNYLKGLADAIL
                     YYGGKIYTETHIQDILAPNEIKTSDGYTTFSKNIVIATNAPIIDKVSKIYDKQQAYRT
                     YVIGARIKKDKIPLGLYWDTGNQNSENTVAPYHYVRIQRHSRKDINSKYDLLIVGGED
                     HLTGNIIDDKDIQQRYTNLESWTRNRFPIEDIEFQWSGQVLEPKDGIAFIGYNPGNEE
                     KKNIFIATGDSGNGITHGTIAGIILTDLIIDKTNPWVSLYNPSRVPREKSNNSQDSKN
                     IKKKEENNNEQESSSKYKDNKMNKPSFQDLDIDQGIVLEEEKIVAYRDSNNKLKLYSA
                     VCTHLGCTIVWNNSEKSFDCPCHGSRFSAATGNTINGPANKKLDEKNYNNN"
     gene            4414..4536
                     /locus_tag="VHJ57_03235"
     CDS             4414..4536
                     /locus_tag="VHJ57_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487576.1"
                     /translation="MSLDVKPDQTKCKECGKLFDTTEGLEQHYELEHKWKNNKI"
BASE COUNT         1746 a          598 c          718 g         1530 t
ORIGIN      
        1 tttttcctta ttaaatggtg ctaaaagcat accaagaatc catacaccga ataaccaaat
       61 tattatcata gtagacatta gtgctggagc cagaaatgcc atactaaagt acattgtagt
      121 aagaatataa tcagatttca tcatattagc aaaaatatca tcaaagcttt tgatacgtat
      181 aatataatat ccaaatacta tacccataac tattataata cctatactga ttccaacata
      241 tacaatattc atagagcagt taagaaaaag aatactaggg tcaatacttt tacaaaagat
      301 gtaactggtt gttatagcca taagaaatga ttttactgca atacttgcaa aaaataataa
      361 cgctttatgt tgtttttcta atatccatct atcattcaat acaaaaattc acttgatgag
      421 tgtgcattac tatatactat gatatgttgt attaagagat tttgataatt gattaccttg
      481 ataaatataa gaaacagaaa ccataaagat tagttttgct attctactta gatttagaac
      541 tatattttta aaaataaaga ataaaggatg aatgactgtc aatataaata actaatttga
      601 ttaacaatag attttataat gtgctttaga attttgcatg taatagtgtt tttattatat
      661 tgtaattggt ttttttatta aatatattaa agttgaatat ataaaatata gagatagaaa
      721 aatgaagatc ttattcaaat ggatatcaat agatggtaaa ttacttcttt gtgcaagaat
      781 tgtaagaaca tttgcatatg gttttttaag cgttattctt gcaatatatt tgaagctaat
      841 tggatttgat gatattctaa ttggaataat tctttctatc acattaataa atagtattat
      901 ttttacatta tttgcaagtt tttatgctga tcgtattgga agaaggaata cgctattact
      961 atatactatt atgatgtcta tttcaggggg catctttttt gtaacagaaa atacattagc
     1021 acttattatt gctgcgttaa tagggacact taatataacc ggatcagaaa caagtgcttt
     1081 tctttctgtt gaacaatcaa tacttcctca gactatcaaa gataatagaa aaaggaatac
     1141 tctttttgga ttttataata tggcaggaac atttgcaatg ggagcaggaa ttctgattgc
     1201 caatttacct attataattc aaaatgaatt agaatttgat caaatttatg caatcaagtt
     1261 attatttctc ttttattctc tgttaggatt atcaatcata ggaatttatc taaaactttc
     1321 ttctgctata gaaattaaag aaaaagaaaa aactttaaaa ccactatcta aaactttgaa
     1381 tccaaagtca aagaagattg ttgcaaaact atcaggatta tttgctattg attcttttgc
     1441 tggaggtttt gctattcaaa ctatagtttc attttggttt ttttctagat ttgatatcga
     1501 tctgtcaaca atttcttata ttttctcaat aggtagtgta cttacagcat tttcatactt
     1561 aattgctgcc aagattgcag ataaaatagg attgataaat accatggtct ttactcatat
     1621 tccatccaat attttattaa tattacttgc ttttgctcct aatcttgaaa tagcaattgt
     1681 attttacatg ataagaatgt ctttatctca gatggacgtt ccaacaagac aatcatatat
     1741 tgtatcaata gtagaagaag aagaaagaac agcatcagcg ggaataacta atttgtcaag
     1801 aaatatagcg caagcgatta gtccttcaat tacaggttat ataattggat tcttatcttt
     1861 atctgctcct tttataatag gagggttgct aaagatattg tatgacatag ttctttacag
     1921 aaactttaga aaaataaagc catcggaaga agaagaagaa gaaaaagaag aagaagaaga
     1981 acgataagat aagactactg atcgtgagat gatagtgaga atcacatttt agcaatctgt
     2041 ttcatctaca tatttctcaa gctgatctgg gataatacca atagcggtgt taaaataaga
     2101 gtctaatgtt atagtaacaa gaagataata ttagtaattt acatttcaac tattaattaa
     2161 aatcttatct ttaaaaaaaa ttaaaataac taaaatctat ttgttatttt tgtatattga
     2221 gagttgtgca gttatctttg gatcagtaat tgtgtagaaa gtataattcc agacgttaag
     2281 ttagttggaa catattctat gaatcctttt ggttgaatag ttcctgaatc aaatattttt
     2341 cctgccatta agtctttagg attttaacca ttagtaaaag aatgatctat tgtatcatat
     2401 tgaatacgat tattcgttaa tattaatagt gctacatgtt taatacttta attttttttg
     2461 atgaaaggtg aaatctacat tataaaagtt gttgttgtca tcatttatta ttgtaaatta
     2521 tgttagattt ataactttaa atgctaatta actttctata gtaagatagt ataactttca
     2581 atcctgtttg aataacttaa ttttcatgtg atagcaaaaa gatttttgag tattaaccgt
     2641 aatattagtt acagtacatt tagttctaga atgaataata atagaaagat gtcgaccaat
     2701 tatagttata aacaaaagga aaattatgat gatgaattcg agaaaaaaac ctctggaatt
     2761 actgaatcac cttggtatga tgagatacag caacaacaac caatgaaatt tgataaattg
     2821 aggagaaata tttctcattc tgaatcagta gatgtatcta ttgttggtgg aggaattgtt
     2881 ggcttatcta cggcatattt gctctcaaaa tcaggaaaaa aagtagttgt atttgaagat
     2941 ggatatttat gcagtggcga gacaggtcga actactgctc atatcacaca tgctttagac
     3001 gacagatatt ataatttaga gaaattacat ggaatggaag gaacacgcct agttgcagaa
     3061 agtcatactt ctgctataaa tatgatagaa gcaatagtaa accaagaaca tattgattgt
     3121 gattttgaaa gattagatgg atttttattt ctggatccga ccgatacgtt ggaatcatta
     3181 gagaaggagc ttgaagcaac tcacagagca ggtatcactg atactgaaat catttcgaag
     3241 gtatctggtt tatcttttga tactggacct tgcttattgt tcccaaatca ggctcaattt
     3301 caaccattaa attatcttaa aggacttgcc gacgcaatat tatactatgg aggaaaaata
     3361 tatactgaaa cacacattca agatatttta gcaccaaatg aaatcaaaac ttctgatgga
     3421 tatacaactt tttcaaaaaa tatagtaata gctacgaatg cacccattat tgacaaagta
     3481 agtaaaattt atgataaaca acaagcttat agaacatatg tgattggagc aagaatcaaa
     3541 aaagataaaa ttcctctagg tctttactgg gatactggaa atcaaaattc agaaaataca
     3601 gttgcaccat atcactatgt cagaattcaa agacatagta gaaaagatat taattcaaag
     3661 tatgatctat taattgtagg tggagaagac catttaactg gaaatataat cgatgataaa
     3721 gatatacaac aacgatatac taatcttgaa tcttggacaa gaaatcgatt tcctatagaa
     3781 gacattgaat tccaatggtc tggtcaagtc ttggaaccaa aagatgggat tgcgttcata
     3841 ggctataatc ctggcaatga agaaaaaaag aatattttta ttgctacagg agattctgga
     3901 aatggaatta cacatggtac gattgctggt attatactaa cagatttaat tatcgacaaa
     3961 accaatccgt gggtttctct atataatcca tcgcgcgttc caagagaaaa gtcgaataat
     4021 tcacaagact ctaaaaatat aaagaaaaaa gaagaaaata acaatgaaca agaatcatcg
     4081 tcaaaatata aagataataa aatgaacaaa ccatcctttc aagatttgga tattgaccag
     4141 gggatcgtac tggaagaaga aaagatagtt gcatatagag actctaacaa caaactaaaa
     4201 ctttattctg ctgtatgtac ccatttaggc tgtactatag tttggaacaa ttcagaaaaa
     4261 tcgtttgatt gtccatgtca tggttctcgc ttctctgctg ctactggaaa tacaattaat
     4321 ggaccagcta acaaaaaact ggatgagaaa aattacaata ataattaaaa atcaaataca
     4381 taaaatatta aagactatta ttattgatta ttaatgtcat tagatgtaaa accagatcag
     4441 acaaaatgca aagaatgtgg aaaattattc gatactactg agggattaga acaacactat
     4501 gagttagaac ataaatggaa aaataataaa atttaagagt ttaaagtgct ctgttaaatt
     4561 aggaaaagaa aaatagatga atagaattta aa
//
LOCUS       DATAFW010000139         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_85837, whole genome shotgun sequence.
ACCESSION   DATAFW010000139 DATAFW010000000
VERSION     DATAFW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85837"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..636)
                     /locus_tag="VHJ57_03240"
     CDS             complement(1..636)
                     /locus_tag="VHJ57_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX2487577.1"
                     /translation="MVDFNTILVFGAHPDDIEIGMGGTITKLAKLGYDVIMVIATLPN
                     FTIADKKDERKLEAISSASILGCKSPEFLDLSQEDILFGRRMVSLIDSLIKKYKTTSV
                     YTQWIGDSHQDHQILTQSVIAASRDVNNLYMYETTIPGGLTEKAFRPQLFIDVTNEME
                     NKIKSLKYFKSQNLRCGPFWIDAIVGRSLYRGYQMNTKYAEAFEIVKTVQY"
     gene            742..1338
                     /locus_tag="VHJ57_03245"
     CDS             742..1338
                     /locus_tag="VHJ57_03245"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX2487578.1"
                     /translation="MLNKFRKHLQPILEKIGILFASTGLSPNIFSLIGFIITILSSII
                     FGINSLQFDPTINFSAIGSLFLLVGGFFDVIDGSVAKITKRSSRKGSFLDSTLDKISE
                     TIIFLGIAIGELANPILCLIAVSSSLLVSYTRSRAETLGIDLSGVGFGERAERILILA
                     VMGLLPFAHSLEYGVIIVIGISIITIIQRIYKTLKILE"
     gene            complement(1339..1626)
                     /locus_tag="VHJ57_03250"
     CDS             complement(1339..1626)
                     /locus_tag="VHJ57_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S26e"
                     /protein_id="HEX2487579.1"
                     /translation="MPKKRTSRGRTKGGKGSSGTVHCSQCGAMVPRDKAKKITGRITL
                     VEPTLAKELRAQGAYISPATDVKFYCVSCAVHRGIVKVRSEVERRVNTRLR"
     gene            1830..2834
                     /locus_tag="VHJ57_03255"
     CDS             1830..2834
                     /locus_tag="VHJ57_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX2487580.1"
                     /translation="MKIALVGAQPQNTLLISKIQKILDTSFIQTIIMTEKTFNLANQV
                     DLVFIIGGDRAILDYFHKVVDESAPIIGIYESESTGFLAQIDVRDLETIIPRLKKNDY
                     RIDEVVRLAVNVDGKEMEPVLNDVAVFPSKSATLMEHILRIDKNDVWHDNSDGVIIAT
                     PTGSTAYSMSAGGPMILQNSKVFLAVSVNSVDNTRRPLIFSDNCTLEISDILSRYHCE
                     VVLDGGRRLGIKKILTCMKHQYPAKLVRLIGDSSVTSLIAKKIKIAEELLNMPPSAKL
                     ILKILEYEGSMSQKDLATKTLLPERTIRLSLGHLVNRGYVKKKTSLRDARHRTYELKI
                     "
     gene            complement(2824..>3291)
                     /locus_tag="VHJ57_03260"
     CDS             complement(2824..>3291)
                     /locus_tag="VHJ57_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019127.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX2487581.1"
                     /translation="LGICFGFQLSIVSFARNVCGIDNANSTEIDPNTCDPVVEFMPEQ
                     KTIHNMGGTMRLGSHQIQINSNTLASKIYSKNFIIRRHRHRFEFNKKYLSMLSKNGMI
                     LSGYSDNEKRTEILEVPNHKFYFGVQYHAEFDSRPGTPEQAFEAFIKNASLKS"
BASE COUNT         1157 a          521 c          501 g         1112 t
ORIGIN      
        1 ctaatattga acagttttta caatttcaaa tgcctcagca tattttgtgt tcatttgata
       61 tccacgatat aaagatcttc caacaatagc atctatccaa aaaggaccgc atcgaagatt
      121 ctgtgatttg aaatacttta aagactttat tttattttcc atttcgttag taacatcaat
      181 aaatagctgg ggcctaaaag ctttttctgt taagccgcct ggtattgttg tttcatacat
      241 atacaaatta ttgacatcac gtgaggcagc aataacagac tgggtaagaa tttgatgatc
      301 ttgatgtgaa tcacctatcc attgagtata aactgaagtt gttttatatt tctttattaa
      361 agaatcaatc aaggatacca ttcttctacc aaacaatata tcttcctgag ataaatctaa
      421 aaattcagga gatttacatc ctaaaataga agcagaagat atggcttcca attttctttc
      481 atctttttta tcggcaatag taaaatttgg tagtgtagct ataaccatga ttacatcata
      541 cccaagcttc gccaatttgg taatagtacc acccatgcct atttcgatgt catcaggatg
      601 agcaccaaaa accaatattg tattgaaatc aaccataaga tcttttaaat aaaatataat
      661 taaattttta ctaatcaaat tcatcaagcc atttaaagat taaaatatag agatactagt
      721 taaaattaac agtctaaatt attgttaaac aaatttagaa aacatttaca accaattttg
      781 gagaagatag ggattttatt tgcatctact ggattatcac caaacatatt ctctttaatt
      841 gggttcatta taactattct ttcttctatt atttttggaa ttaattcttt acaatttgat
      901 ccaacaataa atttttcagc cataggtagt cttttcttgt tagttggggg attctttgat
      961 gtaattgatg gtagtgttgc taagattact aaaaggtcat ctagaaaagg ttcttttctt
     1021 gattctacat tagacaagat ttctgagaca ataatatttt tgggaatagc gataggagaa
     1081 ttagctaatc ccatattgtg tctaatagct gtatcgtcct cattattggt cagctataca
     1141 agatctcgtg cagaaacatt aggaattgat ttaagcggcg taggatttgg agaaagagca
     1201 gaaagaatac tcattcttgc tgtaatggga ttacttcctt ttgctcattc actagaatat
     1261 ggtgttataa tagttatagg aatttctatt attacgatta tacaaagaat atataaaaca
     1321 ttaaaaatcc tggaatgatt atctaagtct tgtatttact cttctttcta cttcagatct
     1381 aaccttgact attcctctat gaacagcaca agaaacacaa taaaatttta catcagtagc
     1441 tggagaaata tatgctcctt gagctctcaa ctctttagct aaagtaggtt caactaaggt
     1501 aatcctacct gtaatctttt ttgctttatc tctaggaacc atagcaccac actgactaca
     1561 atgaacagtt cctgaactac ctttacctcc tttagtccga ccgcgacttg tacgtttctt
     1621 tggcatataa ttatgattct gaatccatac tttaaaggtt ctggtaaatt tactcttata
     1681 aataaaagtc aattaattat attttaattc tgtgcaaacc ttaaaaatca gatgtataaa
     1741 actggagatt accaatgaaa tactaaattt aaaaaaataa caaaaaaatt taactttaaa
     1801 tgagagatta aataactata aagaaaataa tgaaaattgc actagtaggt gctcaacctc
     1861 agaatacgtt attaatctca aaaatacaaa aaattcttga tacttcgttt attcaaacta
     1921 tcatcatgac tgaaaaaaca tttaatctag caaatcaggt agatttagta tttatcattg
     1981 gtggagatcg agctatttta gattattttc ataaagtagt cgatgaatct gctccaatca
     2041 taggaatata tgaatctgaa tccactggat ttttagctca aattgatgta cgcgatttgg
     2101 aaacaatcat tccaagacta aaaaaaaatg attatagaat tgatgaggtt gttcgattag
     2161 cagtaaatgt agatggaaag gaaatggaac ctgtattaaa tgatgttgca gtatttccaa
     2221 gtaaaagtgc tacattgatg gaacatatac tacgtattga taaaaatgat gtgtggcatg
     2281 acaatagcga tggagtaatc atagcaactc ctactggatc tacagcctat tctatgtcag
     2341 caggaggtcc tatgatacta cagaattcaa aagtttttct agcagtatct gtaaattcag
     2401 tagataacac tagaagacca ttaatcttct cagataattg tacattagaa atatcagata
     2461 ttctcagtag atatcattgc gaagtagttt tagacggtgg cagaagatta gggataaaaa
     2521 aaattctaac atgtatgaaa catcaatatc cagcaaaatt agtgaggttg ataggtgaca
     2581 gctctgttac ttcgttaatc gctaaaaaaa ttaaaattgc tgaagaatta ttgaatatgc
     2641 ccccaagtgc caagctaata ttaaaaatat tagaatatga agggtctatg agccagaagg
     2701 atttagctac taaaacatta ttacctgaac ggacaattag attatcttta ggtcatcttg
     2761 ttaatcgagg atacgtaaaa aagaaaacat cattacgtga tgccaggcat agaacatatg
     2821 agctcaagat ttgagagaag catttttaat aaaggcctca aatgcctgtt ctggtgttcc
     2881 tggtctacta tcaaattctg catgatattg aacgccaaaa taaaacttat gattaggaac
     2941 ttctaatatc tctgttcttt tttcattgtc tgaatatcct gataaaatca tcccattttt
     3001 acttaacata gataagtatt ttttgttaaa ttcaaatctg tgtctatgtc tcctaataat
     3061 aaaattcttt gagtatattt ttgacgcaag agtattagaa tttatttgta tttgatgact
     3121 acctaatctc atcgttcccc ccatattatg tatcgtcttt tgttcaggca taaattcaac
     3181 tacaggatcg caggtatttg gatctatttc cgtagaatta gcattatcaa ttccacatac
     3241 attcctagca aatgaaacaa tagacaattg aaatccaaaa catattccaa g
//
LOCUS       DATAFW010000140         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3787989, whole genome shotgun sequence.
ACCESSION   DATAFW010000140 DATAFW010000000
VERSION     DATAFW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3787989"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VHJ57_03265"
     CDS             <1..634
                     /locus_tag="VHJ57_03265"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX2487582.1"
                     /translation="NIYIADFGNPTYAIKKFSKDGNFIDTFGSFGLGDGEFITPGGLG
                     VDKENNIYATDFGENNNVQKFDQNGNFIMKFGTYGTGDGQFLNPTDVAVDSLGNIFVV
                     DSFNNRIQKFDQNGNFIMKFGTPGTGNGEFNTPFELSIDSSDNIFVVDHHNNRVQVFN
                     RDGGYITQFGNQGSAAEKLNDPIGITIDTSGNVYVADGKDTDIHVYSPVK"
     gene            complement(768..1151)
                     /locus_tag="VHJ57_03270"
     CDS             complement(768..1151)
                     /locus_tag="VHJ57_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX2487583.1"
                     /translation="MAKKRVLIIDDDEDINNLFKIYLENRGYQINAYTDPVNALYYFK
                     KGFYDLILLDLKMPQLDGITLYQQLKKIDNNADICLITADIANLEQLKEKIPNIEKYV
                     IYKPILLKNLKEKIDSLLLEKSMSC"
BASE COUNT          571 a          196 c          218 g          600 t
ORIGIN      
        1 caatatctat attgctgatt ttgggaatcc tacttatgct attaaaaaat ttagcaagga
       61 tggaaacttt atagatacct ttggttcatt tggccttggt gatggagaat ttatcacacc
      121 aggaggacta ggtgtagata aagaaaataa catttatgct acagattttg gagaaaataa
      181 taatgtacaa aaatttgatc aaaatggtaa ttttataatg aaattcggta cttatggaac
      241 cggtgacgga caattcttga atccaacgga tgtagcagta gattcacttg gaaatatatt
      301 cgtagttgat tcttttaaca accgtataca aaaatttgat caaaatggta attttataat
      361 gaaattcggt actccaggaa ctggtaatgg agaatttaat acaccttttg aactatcaat
      421 tgattcttct gataatattt tcgtagttga tcatcataac aatcgtgtac aagttttcaa
      481 tagagatggt ggatatatta ctcaattcgg taatcaaggt tcagcagctg agaagcttaa
      541 tgatccaatt ggtattacaa ttgatacttc tggtaatgta tacgtggcgg atggaaaaga
      601 tactgatata catgtgtata gtccagtaaa ataataattt ttacttatat ttattagaaa
      661 tagaatcaga atataataca tgctatagaa ttagcctaaa attagcataa aaatagcttt
      721 ttgtattaat ttattattat ttattaaaat cttaataatt aaactagtta acaggacata
      781 cttttttcca aaagtaatga atcaattttt tcctttaagt tttttaataa gatgggtttg
      841 tatattacat atttttcaat attgggaatc ttttccttta attgttccaa atttgcaata
      901 tcggccgtga ttaaacagat atctgcatta ttatctattt tctttaattg ttggtacaga
      961 gtaatgccat ctagctgtgg catttttaag tcaagtaaaa ttaaatcata aaatcctttt
     1021 ttaaaataat ataaagcatt tactgggtca gtataagcat ttatttggta tcctcgattc
     1081 tctaaatata ttttaaaaag attattgata tcctcatcat catctattat caatactctt
     1141 ttttttgcca tggacatttt ccctaacttt agttcaatat acagtattat ttattaagat
     1201 tggggagaat tactaatatt ttacctttca gtaaacggat attgacatca gaaatcaaaa
     1261 taaagaaaat ttaatctaaa atctttgaaa ttttttgtta ttatatcaat cgttaacaag
     1321 ggcactataa caataataat attgtaaaat gttaaaatga tattttcatg gtagttcaaa
     1381 atttatatta atataaattt gataatataa taaaatggaa agatctaatc ctaatcttat
     1441 tcttttatca acacataatt attgtgaatt aaccattgta caattccatt tttgataact
     1501 ttcgcatatg taataactat atcttttgaa caaaaatttt atatgtctaa atgaaagttt
     1561 aatcctaatc taatactatc atttg
//
LOCUS       DATAFW010000141         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1851331, whole genome shotgun sequence.
ACCESSION   DATAFW010000141 DATAFW010000000
VERSION     DATAFW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851331"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..271)
                     /locus_tag="VHJ57_03275"
     CDS             complement(32..271)
                     /locus_tag="VHJ57_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX2487584.1"
                     /translation="MSSDSINYNPGDIIKKEARGLGEADLGEVQEISGEYIITQKGNI
                     DKDKFYIPKNLLERFDGDTVYLRITEEEAKQYKKD"
     gene            complement(347..673)
                     /locus_tag="VHJ57_03280"
     CDS             complement(347..673)
                     /locus_tag="VHJ57_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487585.1"
                     /translation="MSLDPAIRFVTIFDIDDNIFLFSEHRPGVTNLLTKEESKHSLQI
                     ALGAWKMRRTLENKIGKGKFVLAEYEKIKRITMPLDKNHLLYITTEAGCDALSLVDKI
                     KKLDYK"
     gene            complement(1577..>1906)
                     /locus_tag="VHJ57_03285"
     CDS             complement(1577..>1906)
                     /locus_tag="VHJ57_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487586.1"
                     /translation="MKNFLDYFWSDFAGKLFGYICASHEKGLTVMDQMRTAVRQCYGW
                     SMPYGISINGEKDFSSNGNITNEALSRRIQMLARDIVIYGKLHRNQFLNDLSSQEINT
                     FASHYRH"
BASE COUNT          596 a          286 c          271 g          753 t
ORIGIN      
        1 gataattctt aaaaattaag tttgtaagaa gctagtcttt tttgtattgt tttgcttctt
       61 cctctgttat tcttaaatag actgtatcgc catcaaaacg ctctagtaaa tttttaggaa
      121 tatagaattt gtctttgtct atattacctt tttgagttat tatgtattct cctgaaattt
      181 cttgaacttc accaaggtca gcttcaccta aacctcttgc ttctttttta ataatatctc
      241 ctggattata gtttatagaa tcactggaca tagcaatagt ttagtattac atttatataa
      301 gttctttttg agattgataa attatatcgc tctttcagta ccaactttat ttataatcta
      361 attttttaat tttatcaact aggctaagag catcacatcc agcctctgta gttatataaa
      421 gcaaatgatt cttatctaaa ggcatagtaa tgcgttttat cttttcatat tcagcaagta
      481 caaacttccc ttttccgatc ttattttcga gcgtgcggcg cattttccac gcaccaagtg
      541 caatttgaag agaatgtttg ctttcttctt ttgttaggag atttgtcact ccgggtctat
      601 gttcagaaaa aagaaatatg ttatcatcaa tatcaaatat tgttacaaaa cggatagctg
      661 gatcaagact cataatttgc tctccaattt ttttataatc cattcttaaa tagttagtta
      721 tgtctgtata aaaagtttgt agcataaagt tttcttatct cgttagaatg attctaattc
      781 taactttcta catgacccta ttaatatatc tttgtgcaga gagctataaa tctaatttga
      841 tatttaatca actctagata aaaagcaatg acaaattata agataaagcc taataaattc
      901 tgatagataa ataatgatct acttgcgatt gaaaattagt ataatatatt aatgaattaa
      961 aactatttta catttaatta tataaataaa atatatctct attattccat ctaaatggga
     1021 tttgaagtct atattaattt tttttaatta ctgacaagta tttttctatt ttctcctata
     1081 gaactgaact ctggttacct tgttgttgtt gtgattattg ttgtggtgat attatctgat
     1141 tactaccttc tacgattttt ttttcaaaag gtacaattgt ttggattatt atagttgttt
     1201 ttgcttctca gattggcatt agtattgttt tagttctgaa ccctggaaag attagcatac
     1261 gttgatcttg tctatcatat tttgtaaagg ttaagtgctg acttgtcaat tggtaatgta
     1321 gtttcagata ctcccaaggt gatttactga gaaggaatag ttgtttattt ataattatag
     1381 gttctggtgt actggtaaat tctgccttag tttgattcgt tgaatttcac taaaattaga
     1441 ttgaccaata actggaacaa taaaaatagt tatggatgtg attcaataat aatggtccat
     1501 agtctctaca tctatataga tacagtaaaa attatacaaa tatctatgat taaactctaa
     1561 ttacaaatag caataatcaa tgtctgtagt gagaggcaaa tgtatttatt tcttgggaac
     1621 ttaaatcatt aaggaattga tttcgatgta attttccata tattacgata tctcgagcta
     1681 acatttgtat tctacgtgat aatgcctcgt ttgtaatatt tccatttgaa ctaaagtctt
     1741 tttctccgtt tatagagatg ccataaggca tactccatcc ataacattgt cttactgctg
     1801 tgcgcatctg atccattact gtcaatcctt tttcatgaga ggcacatata tacccaaaaa
     1861 gttttcctgc gaaatccgac caaaagtaat ctagaaaatt tttcat
//
LOCUS       DATAFW010000142         5188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4673212, whole genome shotgun sequence.
ACCESSION   DATAFW010000142 DATAFW010000000
VERSION     DATAFW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4673212"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VHJ57_03290"
     CDS             complement(<1..203)
                     /locus_tag="VHJ57_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487587.1"
                     /translation="MKTIGTVKEDFKMFDKHKNKSSKKVVTASQKEDPIHILDLDDGE
                     FAFMEWLNSVNKTSSKNHIVLKR"
     gene            complement(708..968)
                     /locus_tag="VHJ57_03295"
     CDS             complement(708..968)
                     /locus_tag="VHJ57_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2487588.1"
                     /translation="MGNRSRTEIVAMILNAANGGETKTKIMYFAFLSYNQLKEYLSIL
                     IENNLIEYLDGAFKFRTTEKGLFFLKLHNEMGELLQTTFEDN"
     gene            complement(1847..2131)
                     /locus_tag="VHJ57_03300"
     CDS             complement(1847..2131)
                     /locus_tag="VHJ57_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487589.1"
                     /translation="MENKNLVNDSSYPPDNGLTAAEQLISSYKEFLAKSAKLFPKVDQ
                     KLLLDMIDLETTYKDWEGSVLLKIVYSSNLLVNTEKKRKYIKNIKRCLMR"
     gene            complement(2377..3609)
                     /locus_tag="VHJ57_03305"
     CDS             complement(2377..3609)
                     /locus_tag="VHJ57_03305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487590.1"
                     /translation="MKVNVIHGDFNPCGGAERLSLTTMQALLEKGIDFDLTTLKSPDI
                     LKLENSYGQNLVSVMKRINKINVINILEELRQQQQQEDHKDYNYDITINTNGDAAPYY
                     HSSFSKNNAITYCHFPSTKYHIDSENIEYLKTDLGMMESSKEYKDINNSKNYKTKAQF
                     KRRKEYFKLLKYGYCNLMRNSTVITNSEFSRRAIVNALGLDNIYVLSPPIDVETFHNA
                     ALMANVDNETDDIILVISRIAPHKKIENAIRLAKILKDNNIGKGMKIVGNLYYYFFDY
                     YSELKQMVQDLGLTDYITFEINASLDKLLSIIRESRVYFHPMIGEHFGMAVLEAMAAG
                     LIPVVPNEGGVIEFVPQKYQFNTIEQAAEIIIYVFNHLSKTERIKISDEINKFSNSHY
                     IEGFQNILKELLSRKRKQ"
     gene            4028..5071
                     /locus_tag="VHJ57_03310"
     CDS             4028..5071
                     /locus_tag="VHJ57_03310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487591.1"
                     /translation="MEAIVMWVSKNVASKGHDVTLISARGSSVGEGGGGGEGLPSNFN
                     IIETIEPSWEGTVAEEAHYLMYKELLEKEYGSGNGIVWDNTWHCFSYLSAKKFPNMKI
                     IHTNHGTIEWQKVPVKQLPFPRYIGLSRLHAKYMSSILKIPVRYVHHGIPLPPIEETN
                     NYNHKNGDYLLSINRIVKEKGIEDSIELAVRTRNAIKIAGDDIHVPDLSYVQMIREKC
                     QNTSGLAQYFGLVDNKTKTELIKKCKAVIACPKPSWIEAFGLYAVEANAYGKPVLALA
                     NGGLNDIIVNQTNGFLAKTPRELQEYVEKVYECSPESCRRRVEKLFTDKIMTKNYLHI
                     FEGVMGDEPTFRW"
BASE COUNT         1773 a          771 c          767 g         1877 t
ORIGIN      
        1 tatcttttaa gcacaatatg atttttagaa gatgttttgt ttactgaatt taaccattcc
       61 ataaaagcaa attcgccatc atctaaatca agaatatgga tagggtcttc tttctgagat
      121 gctgttacta ccttttttga agatttgttt ttatgtttat cgaacatttt aaaatcttct
      181 tttactgttc cgatggtttt caatagaatc cattatatag taaatcaacg taataataaa
      241 agaaaatata aagatttgga aagtatcata tatagaaatt tttcatggtt tcatatttaa
      301 atttcttttt tatatttttt tcttgtttgt ttgttaaatg ctttataatt caaaagtatt
      361 attaacatca cacctgtatt tatggcaatc gcatttgaag catccacaaa taatatcaca
      421 ccaaaactaa aagtaaaatt taagtgccct cccctatgaa atatgtaaaa ttttgtatga
      481 gactgtatat aaaggaaaaa cataagaaat taaagaatgt cgaacataaa agggcaagtg
      541 gtataggtga tttaatgaga aataataacg acaattaaat ttattttcca agatcattgt
      601 ttataatatt atcttttgag aattcttctt tttaaaaacc agaatagagc aggtataatt
      661 ataaccaagt tttaagaata tatctggttg atttcacaaa taggtattta gttgtcttcg
      721 aaagtagtct gtaataattc tcccatttca ttatgtaatt taagaaagaa caatcctttt
      781 tctgtagtcc tgaatttaaa tgctccatcc aggtattcta ttagattgtt ttctatcaaa
      841 atagaaaggt attcttttaa ttggttatag cttagaaaag caaagtacat aatttttgtt
      901 ttggtttctc ctccgtttgc agcatttagt atcattgcta ctatttctgt cctacttcta
      961 tttcccacat tattagtaat gtgatttaat tattttagta ttaataccac aagaaaaggt
     1021 aatctaacta tagtaaaaca ttcttcatat tctttacaaa ttaaaattag aacagttcta
     1081 ttactacatt gtattagctt ttattattag aacttatttt ttaaatgttt ttggaatatt
     1141 aagatacgta aattcgttag aaaataatac agatagcggt tcaaaaataa gaatcgcatg
     1201 taaatagaaa ctcctacttt tatttggtag gtattataag aaacttattt ttaaattgat
     1261 atattttaga taaaatgaat aagacaaaca tactttaaaa tatttaaaga atctcaactg
     1321 agagatgaaa attatagacg taaatagtca tttaaacata caacaaatga ataaagtagt
     1381 tattattagt aaaagtggaa atctttcatg tagtatggtc atctaaaaag tatcgtcatt
     1441 taatagttta cgtttctaaa ttttatctat tattagtagg cctattttaa aaatgttagt
     1501 aggtttcagc tacaatgatt tggttcatct atgttttaca gaagttggtg gagagaagat
     1561 tcgtccaggg gagtctaata ttacattata tttagtattg attaatatta aaaaattatt
     1621 taattttgca tattcagatc atcgaatcta atgtaaatta tgcctacaaa aagaaaatct
     1681 ggtattagaa cttttttgtt actatttata ctgaatctga atatgaatct aatggggtta
     1741 aggttgctga accactaagg aactcaatat cattatcact atttattaat tcctaagttt
     1801 ttgatataca ttcttacaag tttaactctc aatgttctac cttctattac ctcatcaggc
     1861 atcttttgat attttttata tatttcctct ttttctctgt atttactagt aaatttgaag
     1921 aatatacgat ctttaataaa acactaccct cccaatcttt atatgtagtt tcaagatcta
     1981 tcatatctaa taacaatttt tgatcaactt taggaaataa tttcgcagat ttagctaaga
     2041 actccttgta agacgatata agctgttctg cagcagttag accgttgtca ggaggatagg
     2101 aagaatcatt aactaaattt ttattttcca tttgatatta tcttattcaa atttaaaatt
     2161 attttctaca aaatccgaat cttcttttat atataattcc attgttaatt gattgaagtc
     2221 tttgattaaa gatatttatc cttacttatt gagccaatat ctttatatca ttaatagtta
     2281 atctcgaatg aataattaca atcatcaaat tatgattgtg agtagcaaaa atagtgtatc
     2341 attcatttaa acaagtaata cttatactag taaccatcat tgtttccttt ttcgagataa
     2401 taattccttt aatatattct gaaatccctc aatataatga gaatttgaga atttattaat
     2461 ttcatcactt atcttaattc gctctgtctt tgacaaatgg ttaaaaacat atattataat
     2521 ttcagcagcc tgttctattg tattaaattg atatttttgt ggaacaaact ctataacgcc
     2581 accttcattt ggaaccactg ggattagtcc agcagccata gcttctaata ctgccatacc
     2641 aaaatgctct cctatcattg gatgaaagta tactctggat tctcgtatga tagaaagtag
     2701 cttatctaag ctagcattaa tttcaaatgt aatataatca gtcaaaccaa gatcttgaac
     2761 catctgctta agttctgaat aataatcaaa aaaataatag taaagatttc caacgatttt
     2821 cattcctttg cctatattgt tatcttttaa tatttttgca agcctgattg cattttctat
     2881 tttcttgtga ggagcaattc tggatattac aagaatgata tcatctgttt cattatcaac
     2941 atttgccatt aatgcagcat tatgaaaagt ctcaacatct attggtgggc ttaatacgta
     3001 tatgttatct aaccccaatg cattaactat tgccctgcga ctgaattcag aattggtaat
     3061 cactgtagaa ttcctcatca aattgcaata tccatatttt aataatttaa aatattcttt
     3121 tcttctcttg aactgagcct tagttttgta attttttgaa ttattgatat ctttatactc
     3181 ctttgaactt tccatcatac caagatctgt ttttagatat tcaatatttt cagagtctat
     3241 atgatatttt gtactaggaa aatgacaata agtaatagca ttattttttg aaaaagatga
     3301 atgataataa ggagctgcat caccatttgt atttattgta atatcataat tgtaatcttt
     3361 atgatcttct tgttgttgtt gttgccgaag ttcttcaagt atatttataa cattaatttt
     3421 gtttatacgt ttcataactg agacaagatt ttggccatat gaattttcta attttaatat
     3481 atcaggactc ttcaaggttg taaggtcaaa atctattccc ttttctaata atgcctgcat
     3541 agttgtaagt gatagtcttt ctgcgcctcc acagggatta aaatctccgt gtataacatt
     3601 tactttcata tagagcaaca tatcctgcta aggccaaact ctctcgctat ctttttcact
     3661 cgtgttttct tcttctttat aggctcacaa agtatttaga gaagagtatt atagacgttt
     3721 gctctgaaaa gtaacgatca tttccctgtt atatgaatac aaaaagaagt aaccactttt
     3781 tatatccttt agttactttc cttttttcta tatggaaaaa taggtatcaa agcatttcaa
     3841 acatcattaa ttgttgtgat aataatacaa tagaccatta agtaatctct agaatggtta
     3901 ttattaacat caatgctcat acttaatggt gttaataaac gaaagtatta gttataagta
     3961 gccctaattt accgacccca cctcccgatt attttcatct cacacttgtt gaagatcgaa
     4021 tggaggcttg gaggcaatcg ttatgtgggt ttctaaaaac gtggccagta aaggtcatga
     4081 tgtgactttg atatcagcta gaggttcttc tgtaggtgaa ggaggaggag gaggagaagg
     4141 actgccatcc aactttaaca taatagaaac gatagaacct tcctgggaag gaacggttgc
     4201 tgaagaagca cattatctca tgtacaagga actactagaa aaagaatatg gaagtggaaa
     4261 tggaatagta tgggataata cctggcattg cttttcctat ctctctgcaa agaaatttcc
     4321 aaatatgaaa ataattcata ccaatcatgg tacaatagag tggcaaaaag ttcctgtcaa
     4381 acagctccca ttccctagat atattggact atcgaggctt catgctaaat atatgtcgtc
     4441 tatccttaaa atacctgtca gatatgtcca ccatggaatt cctcttcctc cgatagagga
     4501 aacaaataat tataatcata aaaacggaga ttatcttctc tcgataaata gaatagtcaa
     4561 agaaaaagga attgaagatt caatagagtt agcagtgaga acaagaaacg ctataaaaat
     4621 agcgggtgat gatatacacg taccagatct ttcatatgta caaatgataa gagaaaaatg
     4681 ccaaaatact agtggcttgg cacagtactt tgggctcgta gacaataaaa ctaagacaga
     4741 attaatcaag aaatgtaaag ctgttattgc atgtccgaag cctagctgga ttgaggcctt
     4801 tggactatat gctgtagaag cgaatgccta tggtaaacct gtacttgctc tagctaatgg
     4861 aggactaaac gatataattg taaatcaaac aaatggtttt ctagctaaaa ctcctcgaga
     4921 attacaagaa tatgtagaga aggtttacga atgttcgcca gaatcttgta gacgtagagt
     4981 tgaaaagctc tttacagata aaataatgac taaaaattat cttcatatct tcgaaggggt
     5041 aatgggagat gagcctacgt tcagatggta gtaatagtag gtagttgttt tactgccact
     5101 atggcttgac tatcctatgc attatagatt gattatcaaa actagaaatt tgtgacgtag
     5161 taataacttc agtttgggcc tcacaata
//
LOCUS       DATAFW010000143         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2911008, whole genome shotgun sequence.
ACCESSION   DATAFW010000143 DATAFW010000000
VERSION     DATAFW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2911008"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VHJ57_03315"
     CDS             <1..585
                     /locus_tag="VHJ57_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487592.1"
                     /translation="LEFYDKLGLYSIEVLSNQYGISRLYCGFNSSFIISSVSDFKSNT
                     KFNFNEQKSLIISAGAEPENHTTLNNSLYSLWNSIGILKNQGMAVLLAENNHGIGDGA
                     LTLYLEGKLNPSQIKKINYVNGLEHLIYLENLRNEYYLGLVSSLPFHYTKTKLGFNTF
                     NNVKEVLQNMLTRYGKSNKIMICPDPSITFLIKQ"
     gene            687..1184
                     /locus_tag="VHJ57_03320"
     CDS             687..1184
                     /locus_tag="VHJ57_03320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX2487593.1"
                     /translation="MQSILRSVDSYVIRNCEEKDLPFVININMITLPEHYSEYFFSSI
                     LAELPEAFIVAEIKNQIVGYIMCKIEFGFSNFRKLGFVKKGHVVSVAVLEEHQGKGVG
                     TALMLEGMNGVIIRKSDEIYLEVRVSNESAVQLYKKLGFIIKSRLKSYYRDGEDAFLM
                     AKDLS"
     gene            1218..1436
                     /locus_tag="VHJ57_03325"
     CDS             1218..1436
                     /locus_tag="VHJ57_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487594.1"
                     /translation="MKSNRPTGIFLLIIPPVIFLIYIYLLFASSLDLLLIKLTIVGTA
                     GIIVSVFIWIGYTMISTSNANEITDDNI"
     gene            complement(1433..2275)
                     /locus_tag="VHJ57_03330"
     CDS             complement(1433..2275)
                     /locus_tag="VHJ57_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX2487595.1"
                     /translation="MGSMLKNALKDVFSKEEIEKVYSSFDVIGDIIIIKIPEELDGKK
                     EIIAKILINKIKTVKTVFQQISSVQGEYRTRRLCFLTGINKSITEYREHGCIFKVDVL
                     KTYFSPRLSTERLRVSKLIRNNEIIVNMFAGIGTYSIILAKKNPDCIVYSIDSNPYAN
                     ELCIINSKLNKVQDRVIPILGDARTIVSTQLKGKATRVLMPLPEQAAEFIDSAIMALE
                     NNKGIIHYFAHIKAKSKLNALEEGKLNCHNIFKCYQYKIICGRVIREVGPRIYQLIFD
                     LEII"
     gene            complement(2288..>2648)
                     /locus_tag="VHJ57_03335"
     CDS             complement(2288..>2648)
                     /locus_tag="VHJ57_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020915.1"
                     /note="originally found to be an inhibitor of the
                     antiviral RNase-L in human cells; contains ABC-type
                     nucleotide binding domains; putatively functions in RNA
                     maturation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HEX2487596.1"
                     /translation="DIYALDEPSAFLDIEERIALAKFIQRFVRAQGKSAIIIDHDMQL
                     IDIVSDSLIIFNGQPSLYGIASKPMFKIEGMNLFLKELAITYRRDETTGRPRVNKNGS
                     QLDKLQKESGEYYYIKR"
BASE COUNT          895 a          385 c          362 g         1006 t
ORIGIN      
        1 ttagagtttt atgataaatt aggtctatat tctatcgagg ttttatctaa tcaatatgga
       61 atttctcgtc tttattgtgg atttaattca tcatttatta tatcctcagt ttcagatttt
      121 aaatcaaata cgaaatttaa tttcaatgag caaaagtcat taataatcag cgcaggtgcg
      181 gaacctgaaa atcatactac tcttaataac tcattatatt cattatggaa ttctatagga
      241 attttgaaaa accaaggaat ggctgtttta ctagcagaaa ataatcatgg aataggtgat
      301 ggtgctttaa ctctgtattt agaaggtaaa ttgaaccctt ctcaaataaa aaaaatcaac
      361 tatgtaaatg gattagagca tctaatttat cttgagaatt taaggaatga gtattatctt
      421 ggcctagttt cgtcattacc tttccattat actaaaacaa aattaggatt taatactttt
      481 aataatgtta aagaagtttt acaaaatatg cttacacgtt atggaaaatc aaataagatt
      541 atgatttgcc ccgatcctag cattacattt ttaataaaac aataagaatt atcttaattt
      601 ctgagatatc tttatctatt tactttataa taaagaattt gttctattat ataaagttaa
      661 ataccttgtc aagttaaata tagaaattgc aatctattct tagaagtgtt gatagttatg
      721 ttattcgtaa ttgcgaagaa aaagatcttc catttgtaat taatataaat atgataacac
      781 ttccagaaca ctattctgaa tattttttta gttctatttt agcagaatta cctgaagcat
      841 ttatcgttgc ggaaataaaa aatcagatag ttggttacat catgtgtaaa atagaatttg
      901 gattctcaaa tttcaggaaa ttaggttttg taaagaaggg ccatgtagta tccgttgcag
      961 ttttagaaga acatcaagga aaaggagtag gaacagcatt aatgctagaa ggaatgaacg
     1021 gtgttattat tagaaaaagt gatgaaatct atttggaagt tcgagttagc aatgaaagtg
     1081 cagttcaact atacaaaaaa ctaggtttca ttataaaatc tcgattaaaa tcctattatc
     1141 gtgatgggga agatgcattt ttaatggcaa aagaccttag ttagatatat aatatttaaa
     1201 ttggttttat tatgataatg aaaagtaata gacctactgg catttttctt ctaattattc
     1261 ctccagtaat ttttctaata tatatatatc tattatttgc ttcaagtcta gatttgcttt
     1321 taataaaact taccattgta ggaactgcag gaataattgt ttcagtattt atatggattg
     1381 gatatactat gatttctact tctaatgcta atgaaattac agatgataat atttaaatta
     1441 tctctaaatc aaaaatcaat tgatatattc taggaccaac ctcccttatt actcttccac
     1501 aaattatctt atattggtaa catttgaaaa tattatggca atttagtttt ccttcttcta
     1561 gtgcgttcaa tttacttttg gctttaatat gcgcaaaata atgaataatt cctttattat
     1621 tttcaagggc cataatggct gaatcaataa attctgctgc ctgttctgga agtggcataa
     1681 gaactcttgt agctttacct tttaattgag tagatacaat tgttctagca tcacccaaaa
     1741 tagggataac tctgtcttga actttgttta actttgaatt aataatacat aattcatttg
     1801 cataaggatt agaatctatg ctatacacaa tacaatcagg attttttttc gcaagaataa
     1861 ttgaataagt tcctatgcct gcaaacatat ttacaattat ttcattattc cttatcaact
     1921 ttgaaactct aagtctttca gtagaaagac gaggtgaaaa atatgtcttt aatacatcta
     1981 ctttaaatat acatccatgt tctctatatt ctgtaattga tttattgatc cctgttaaaa
     2041 aacatagtct tcttgttcta tactctcctt gtactgaaga gatttgctga aagacagttt
     2101 ttactgtctt aattttattt atcaatattt tggctataat ttcttttttc ccatctagtt
     2161 cctctggaat tttaattatt attatatcac caattacatc aaatgatgaa taaacctttt
     2221 cgatttcttc ttttgaaaag acatctttta atgcattttt aagcattgat cccatattcc
     2281 attagcttta tcttttgatg taataatatt ctcctgattc tttttgtaat ttatctagtt
     2341 gacttccatt tttatttaca cggggccgtc cagtagtctc atcccttcta taagtaatag
     2401 ctaattcttt aagaaatagg ttcataccct ctatcttaaa catgggtttg ctcgctatac
     2461 cgtataaact tggttgccca ttgaatatta ttaaagaatc agaaacgata tcaattaact
     2521 gcatatcatg atcaataatt attgctgatt ttccttgcgc tcttacaaac ctctgaataa
     2581 attttgctaa tgctattctc tcctcaatat ctaaaaaagc tgaaggttca tctaatgcat
     2641 agatatca
//
LOCUS       DATAFW010000144         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4499821, whole genome shotgun sequence.
ACCESSION   DATAFW010000144 DATAFW010000000
VERSION     DATAFW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4499821"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..>1874)
                     /locus_tag="VHJ57_03340"
     CDS             complement(83..>1874)
                     /locus_tag="VHJ57_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX2487597.1"
                     /translation="VSNKPIRSHKESIDILVSFDAETLFRHAKDVSTDGIIIFNSELI
                     ETSINEIPTLDSNVQSNIIEYCNYHGEDPFTIKKFIEKIQQNSIKCYGIPYHQLLKNF
                     SEHTNNPALSKLARMINVLALSISLSFININRQALSRAIKYIFRTKPKIAEINVAAAE
                     YAYNYAEQIFQNNKFTFSENKRDFSDIILVQGSQSSALGKIVAGCRFQTYYPITPASE
                     DSEFLETNQVIEQIDGNKGSVLVMQTEDEISAITMAIGGALTGTRTATATSGPGFSLM
                     AEALGWAGINEVPLVVSLYQRAGPSTGLPTRHEQGDLLFAINAGHGEFPRIVFASGDI
                     EESFYDTLKVFNFAEIFQLPVIHLLDKAIASSVMTCKNFDPNKISIDRGYFLKKIDNK
                     LQDHEKLKHFKRFELKENGPISPRPPLGTENGIFWNTGDEHDEEGHISEDPTLRIKMM
                     DKRMSKLHLILQEIQDEDKALSYNDANSDIVITSWGSTKGAILDTLDELNKEGIKIKF
                     VQVKLLNPFPQQLLERMLNSAKTIINIEMNYSSQLAKLIKQNLHRDIDYEIVKYNGRP
                     ISCDELYRVIKGIVNNNNTDKRRIVLDYGT"
BASE COUNT          577 a          324 c          249 g          724 t
ORIGIN      
        1 catttgtatg gcatttaata ttccaaagtc cccacagcct ggacaccaat cattatgtac
       61 ttctgtttta taatctaccg gtttaagtgc cataatctaa aacaattctc ctcttatcag
      121 tattattatt attcactatc ccctttatta ctctatataa ctcatcacac gaaataggcc
      181 taccattata tttgactatc tcataatcta tatctctgtg caaattctgt ttgataagct
      241 ttgctaattg agatgaataa ttcatttcta tgttaataat tgttttagct gaatttaaca
      301 tcctttctaa taattgttga ggaaaaggat ttaataattt aacctggaca aattttattt
      361 ttattccttc tttgtttaac tcatctaagg tgtccagaat agctcccttt gtggatcccc
      421 aactagtaat tacaatatca gaatttgcat cattatatga taaagctttg tcttcgtctt
      481 gtatttcctg taaaatcaaa tgtagtttac tcatacgttt atccatcatt tttattctaa
      541 gtgtaggatc ttctgaaatg tgtccttctt catcatgctc atcaccagta ttccaaaaaa
      601 tcccattctc tgtaccaagt ggtggtctag gagaaattgg tccattttct ttcaattcaa
      661 atcttttaaa atgttttaat ttttcgtgat cttgtaattt attatcaatt ttcttaagaa
      721 aatatcctct atcaatacta attttatttg gatcaaaatt tttacaggtc attacactac
      781 ttgctattgc tttatctagt aaatgaataa ctggtaattg aaaaatctct gcaaaattaa
      841 atacttttaa tgtatcataa aaactttctt caatatctcc cgatgcgaac actattctag
      901 gaaattctcc atgaccagca ttgattgcaa ataataaatc tccttgctca tgtctagtag
      961 gaagacctgt tgatggacct gctctttgat ataatgatac tactaatgga acttcattga
     1021 tacctgccca tcccaaggct tctgccatta atgaaaaacc aggtccagag gttgcagttg
     1081 cagttctagt tccagtcaaa gcaccaccaa tagccatggt tattgcagaa atttcatctt
     1141 ctgtttgcat taccaatact gaacccttat tgccatctat ttgttctatt acttgatttg
     1201 tctctaaaaa ttcactatct tcacttgcag gtgttatagg atagtatgtc tggaatctac
     1261 atccagcaac tattttacct aatgcagatg attgacttcc ttgtactaaa ataatatcag
     1321 aaaaatccct tttattttca gaaaaagtaa atttattatt ctgaaatatt tgttcagcgt
     1381 aattataggc atattccgca gctgcaacat tgatttctgc gatttttggc ttagttctaa
     1441 atatatattt tattgcacga gataatgctt gacgattaat attaataaat gataaagata
     1501 tagataaagc caagacattt atcattctag caagtttact caatgcagga ttattagtat
     1561 gttctgaaaa gttttttaat aattgatgat atgggattcc ataacatttt atagagtttt
     1621 gttgtatttt ttctataaat tttttgattg taaatggatc ttctccatgg taattacaat
     1681 attctattat attgctttga acgtttgagt ctaacgtcgg aatttcattt atcgatgttt
     1741 caatcaattc agaattaaat ataatgattc catcagtaga cacatctttt gcatgtctaa
     1801 ataaagtctc ggcatcaaaa gatactagaa tatctataga ttctttatgt gatcgtattg
     1861 gtttatttga tact
//
LOCUS       DATAFW010000145         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_801108, whole genome shotgun sequence.
ACCESSION   DATAFW010000145 DATAFW010000000
VERSION     DATAFW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801108"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VHJ57_03345"
     CDS             <1..1005
                     /locus_tag="VHJ57_03345"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2487598.1"
                     /translation="IKAKSLDLLRGLLPASTLTNLGITGNGRAFEYLLTRLYASNLTE
                     MNVVSDQLYEELNLIIPSFVYRAKNQHGMSMQNYLRNSKDVILKMAKEHLSHIKINPN
                     LECVNLIFNEDNTEAEVKIASSILFEQAAGHTLTNIIEYVRSLPIEDRHKIIKTYTSL
                     RANRRQRPGRAYEMTEYTFEMLTNFGMFRDLHRHRILTLERQLLSTRHGYDVPKEISD
                     LGILDDFKDCMYKCNEVFQGLVNADYPEEAQYVVNFAYKYPFFMKLNLREATHMIELR
                     TLSQGHPDYRYVCQDIFKKIRNVHPILSEGIRFVDLKDYSLERLSSEKSIEKKKREIR
                     "
     gene            complement(1098..2120)
                     /gene="fen"
                     /locus_tag="VHJ57_03350"
     CDS             complement(1098..2120)
                     /gene="fen"
                     /locus_tag="VHJ57_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HEX2487599.1"
                     /translation="MGIDLKPILTTCPIKSSDLSDKIIAVDAYNTIYQFLATIRGPTG
                     DSLTNNKGEITSHLSGLFYRNINFLTENIKFVYIFDGKPHSLKFNEIKRRKESKQEAI
                     EKYQEALEEGRLEDAKKYSQGTVILTDEIIEQSKKILKMLGIPVIQAPSEGEATASIL
                     TKDGTVYACASQDYDSLLFGAKRLIRNLTITGKRKIPNQNRYVSIEPEIIHQSQVLES
                     TKLTLEQLVDVAILIGTDFNTGGLPGIGPKTALKLIREKGRLEEIEKIKDNLKHVPYQ
                     QIREIFLNPENISIGNIEFDYPNYKEIIDFLCDTMDFSKERVESSLARLKKSQEKRSQ
                     SLERWF"
     gene            2291..2620
                     /locus_tag="VHJ57_03355"
     CDS             2291..2620
                     /locus_tag="VHJ57_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487600.1"
                     /translation="MNTNEKSTLFRSIIVIFFLSMFYLAPILAQNSTSITESHLVGTC
                     QAFTKLQNEKLNLEKKLNETKQNFLPNLIQEKSLTLKLDDINSKLAFLTDNRYCSNLE
                     KQITDQN"
     gene            2751..>3387
                     /locus_tag="VHJ57_03360"
     CDS             2751..>3387
                     /locus_tag="VHJ57_03360"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487601.1"
                     /translation="MKDNIHVVFWNLGNLFDIQPNEISSNLEFTPNKGWNEKVRDIKI
                     QNLTNIIKSTFDNGPDLIGVCEIENAFLAEKLIDELKNVLGRDDYQIAGTNIDGKLKP
                     YHKGPDLRGIDTCLIYSNKIFEQLDGHGYMINFRFPTRDIFYAKLRVLKNNAKLNVLV
                     NHWPSRRGRDDYSELMDTEYSRYMAAEHCGRIVDSILKYPKMEIEKFPNSVD"
BASE COUNT         1199 a          464 c          492 g         1232 t
ORIGIN      
        1 ataaaagcta aatctttaga tcttttacgg gggcttttac ctgcatctac tcttactaat
       61 ttaggtatta ctggtaatgg aagagctttt gaatatcttt taacaagatt gtatgcatcg
      121 aatttaacag agatgaatgt cgtatctgat caattatatg aagagcttaa tctaattatt
      181 ccgtcttttg tttatagggc aaaaaatcag catggaatgt ctatgcaaaa ttatttaaga
      241 aattcaaagg atgttattct caaaatggct aaagaacacc tttcacacat caaaataaat
      301 ccaaatctag aatgtgtgaa tttaattttt aatgaagata atactgaagc agaagtaaaa
      361 attgcatcat ctattttgtt tgaacaagca gcgggacata cacttacaaa tattatagaa
      421 tatgttcgat ctttaccaat agaggataga cacaaaataa ttaagacata tacaagttta
      481 cgagctaata gaagacaacg tccaggcaga gcttatgaaa tgactgaata tacatttgaa
      541 atgcttacta attttggaat gttccgtgat ctacatcggc atagaatatt aactttggaa
      601 aggcaattac tttctactag acatggatat gatgttccta aggaaatttc cgatcttgga
      661 attttggatg atttcaaaga ttgtatgtat aaatgtaatg aggtatttca aggtcttgtc
      721 aatgctgatt atccagaaga agcacaatat gtagtcaatt ttgcgtataa atatccattt
      781 tttatgaaat tgaatttacg agaagctacg catatgattg aattgagaac tttatcacag
      841 ggacatcctg attatcgtta tgtttgtcaa gatattttta aaaaaataag gaatgttcac
      901 ccaatcttat ctgaaggaat aagatttgtt gatcttaagg attatagtct agaaaggcta
      961 tcaagtgaaa aaagcataga aaagaaaaaa agagaaatcc gatagacaat taaacaacat
     1021 ttttacattt gttatttatt ttcaagattt gtgactatca attgtatata tgaatatttt
     1081 tattattttt attattatta aaaccaacgt tctaaagatt ggcttctttt ctcttggctt
     1141 ttttttaatc ttgctagtga tgattctact cgttcctttg aaaaatccat tgtatcacat
     1201 agaaaatcta taatctcctt ataatttgga taatcaaatt ctatattacc tatagaaata
     1261 ttttctggat taagaaatat ttctcttatc tgttgatatg gaacgtgttt taaattatct
     1321 tttatttttt ctatttcttc caatctaccc ttttctctta ttagctttag tgcagtttta
     1381 ggacctatac ctgggaggcc accagtatta aaatcggttc ctattaggat agctacatct
     1441 actaattgtt caagcgtcaa ttttgtagat tccaaaacct gtgattgatg aataatttct
     1501 ggttctatac ttacatatct gttttgatta ggaattttgc gttttcctgt tattgttaga
     1561 ttacgaataa gtcttttagc tccaaaaagt agagaatcat aatcctgact cgcacatgcg
     1621 tataccgttc catctttagt taaaattgat gctgttgctt ctccctctga tggtgcttga
     1681 ataacaggaa tccctaacat ttttaatatt ttcttggatt gctcaataat ttcatccgtt
     1741 aaaattactg tgccttgact atatttttta gcatcttcaa gtctaccctc ttctaatgct
     1801 tcttgatatt tttctatagc ttcttgttta gattcctttc ttcgttttat ctcattaaat
     1861 tttagtgaat gaggttttcc atcaaaaatg tatacaaatt ttatgttttc agttaagaaa
     1921 ttaatgtttc tataaaataa accacttaag tgacttgtta tctctccttt gttattagta
     1981 agagaatctc ccgtaggacc acgaatagtt gctaaaaatt gataaatagt attatatgca
     2041 tctactgcaa ttattttatc tgataaatcc gatgatttta ttggacatgt agttaaaatt
     2101 ggttttagat ctattcccat ttgttaagca tatatgtatt taatgtacct tttatttttt
     2161 catattgtat tattcagata atagagaatg tgattgttgt catatcaaaa taaaaataac
     2221 catgagatga ggattattat agatttgtat aatagtgaga atatataatt cacaatagat
     2281 agataaaatt atgaatacaa atgaaaagtc aactttattt agatccatta ttgtgatttt
     2341 tttcctttca atgttttatc ttgctccaat actagctcag aattctactt caataacaga
     2401 atcacatcta gtcggaacct gtcaagcatt tacaaagtta caaaatgaaa aacttaacct
     2461 tgaaaagaaa ctgaatgaaa ctaaacaaaa ttttctacct aatctaatac aagaaaaatc
     2521 tttaacttta aaactagatg atattaattc taagcttgca ttcttgactg acaatagata
     2581 ttgtagcaat cttgaaaagc aaataacaga tcaaaactaa ttcaaaaata actttttgat
     2641 aaatttaaca tcattgttat tattcataga atctttaatt aactctatat atatatataa
     2701 taaaataatg tctgtaaatt ttatatatta aataaaatct tattattaaa ttgaaagata
     2761 atattcatgt agttttttgg aatctaggaa acttatttga tattcaacct aatgaaatct
     2821 cctcaaatct tgaatttact ccaaataaag gttggaatga aaaagttcgt gatataaaga
     2881 tacaaaattt aacaaatata attaaaagta ctttcgataa cggcccagat ttgataggtg
     2941 tgtgtgaaat tgaaaatgcc tttttagctg aaaaactaat agatgaatta aaaaacgtat
     3001 tgggtagaga tgactatcaa atagctggaa caaacataga tggtaaatta aaaccatacc
     3061 ataaaggtcc agatctcaga ggaattgata catgtttaat ttattctaat aagatatttg
     3121 aacagcttga tggtcatgga tatatgataa attttagatt tcctactcga gatatttttt
     3181 atgccaagct tagagtcttg aaaaataatg ctaaactaaa tgttttagta aatcattggc
     3241 catcaagaag aggcagagat gattattccg aacttatgga tacagaatat tctagatata
     3301 tggcagcaga acactgtgga agaatagttg actctatttt aaaatatccg aaaatggaaa
     3361 tagaaaaatt cccaaatagt gttgatt
//
LOCUS       DATAFW010000146         8949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2210593, whole genome shotgun sequence.
ACCESSION   DATAFW010000146 DATAFW010000000
VERSION     DATAFW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210593"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1242
                     /locus_tag="VHJ57_03365"
     CDS             19..1242
                     /locus_tag="VHJ57_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orc1/cdc6 family replication initiation protein"
                     /protein_id="HEX2487602.1"
                     /translation="MSDDILDNIFENAVKGNSLIKNRKILTIDYIPQKLQFRDEETTT
                     IAQTLAVSLKGARPSNLLLFGKPGTGKTAVVKNVINKFKEKSIDLGVEVIVVFINTKI
                     ANTAYKVLYEIAEEMNINKIDKKNQVYFTGLSLGEATNRILDFIKKRQLHVIFVMDEI
                     DSLVDKNGDDILYNFTRANEQFQEVGFISIIGISNSLTFKDKLDPRVRSSLSEEEIVF
                     NPYKIHQLQKILSERVKLAFHDGVVTEGTINLCAAIAGKDNGDARKAIDLLRVAAEIA
                     EIDKDTKVYEKYIRLAQHKIEKDTNFEIIKNSTTHTKIVLLAIAKSKNGNTGEVYEIY
                     SSLCRHIDSESLTQRRITQIINELDQLGLISTNIVNQGRYGRSQKIKITIPISTLKEA
                     LFDDHLLSTITTTID"
     gene            complement(1232..2827)
                     /locus_tag="VHJ57_03370"
     CDS             complement(1232..2827)
                     /locus_tag="VHJ57_03370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HEX2487603.1"
                     /translation="MSFQKEISNALSYITAHGYQIHPEAFKILKNLDSNIMEIIQKVV
                     RFKKNQNDTNIILIEDIRNFCTINISNEFDTLTVSDSKLQDKNIIKTTINETQEFEII
                     STSEITINKPNYRESFKIIYDSADYIDPEEGINGYISLFKSRFHQSLKILSTRPESKR
                     INKISSIKRKIKQEKNNNYLENNLSVNGNTSIIAGLVMSKRSKKNNIELAIDDLTGII
                     NTIALNEETIKLVSKLTLDQMVMLEIENKGINHVIKNVISPDIPDHIPNKGKSDGYVV
                     LISDLHIGSKYFKEQEFLKFLNWLNSSDNEIVSKINFLCIGGDLIDGVGIFPNQEKEL
                     NDSTIYQQMSHLTSLLEQIPKKIQVFVIPGNHDLGRRALPQPAIPKKDAGILYSWKNF
                     SMLGNPSYIELNGVKILMYHGQGLDDIIATIPGLSYSNPAEAMKTLLKARHLSPIYGQ
                     RTPIAPEKDDMMVITEIPDIFHSGHVHVMGVQNYRGTLIVNSGTWQDQTPFQQTMGII
                     PTSGIAIAVNLSTLRPFQIDFSQ"
     gene            2924..3439
                     /locus_tag="VHJ57_03375"
     CDS             2924..3439
                     /locus_tag="VHJ57_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEX2487604.1"
                     /translation="MKKRLYYLAVGMLVFLISYSIGAKINIEKEQSSLIKEQFETKIQ
                     GIDEIGIFQNNVLIALSMFIPGVGVGIGIFSGVGTGLVFNAMALENTTLQNISPLAIL
                     ITPFGIMEIFSYGLAISRSGMLIYDLFKKKSWKLLLKPTIIEIVIVVSILIVAAFIEW
                     NIIKQFMEISN"
     gene            3715..4452
                     /locus_tag="VHJ57_03380"
     CDS             3715..4452
                     /locus_tag="VHJ57_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX2487605.1"
                     /translation="MLGVKIHYKPKFDKKPILIAALPDMGNVAGLCLNFLINKIDTKI
                     FAEIYSFWPPFVTYKKGIINYLQSSYKFYENSQYNIILFTGDFNPTDPRRLYELCYKV
                     LKIAEMFNVKLLYSLGASLKPINTIEKLVYAGVNKPEMVNVLNELNLPILEDKGQITG
                     FNGLILGIAQEFNLDGICLLGEIDNPNVIQPKTSQEILKVLLKIINFPSIDLSELEEE
                     EKRKKFMEKQMNYFNKAVNQSELPGIT"
     gene            complement(4483..5211)
                     /gene="cbiE"
                     /locus_tag="VHJ57_03385"
     CDS             complement(4483..5211)
                     /gene="cbiE"
                     /locus_tag="VHJ57_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015021032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HEX2487606.1"
                     /translation="MPKKLFVIGVGPGDPDYITQIAKEKILISKYIIGYRYTLRTIEN
                     LIDKEKQQIYEITMKNQESAYQYVYGNMQDNEYCTVPFTGDVSFSESEVVDRLLEIFG
                     NENVEIIPGISSIQVASSKSRVPLDKAFVVTFHVTGDIQQKKIELIKSVLDRKSVILL
                     PRPWPNDLSKNFMPSEIALFLKNNGVNTKEIDVWIFEYLTDKEKENIFRGKMMDLENK
                     TFSDLSVMVIDQYKKETYLNFNLL"
     gene            5379..6353
                     /locus_tag="VHJ57_03390"
     CDS             5379..6353
                     /locus_tag="VHJ57_03390"
                     /inference="COORDINATES: protein motif:HMM:NF037920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdvA-like protein"
                     /protein_id="HEX2487607.1"
                     /translation="MSELKLEFVGKQVKDMYGTLMGKVVGVITDTDGSIESVSVDCGD
                     SGLKQLSYEQLLVQGDYVIFIPKWRLDAQKLFRQKNLTLKRIKALQHLVEEDDTMKVD
                     AEIVYVKYEKKLDELEEKEKSINEKLKERLDELNESTKNIKSILFDAKLQYKSHEISE
                     ETYLQVTRYTKELMDHINLEKEEINNIKSRLEEQNLENTLPLPHSIQPEEEKDNEKEN
                     TNLSTTNTKPVSPTITNTPNTSSSSTVTASNTNNSNSSNTSLPVSSTSNDNTQTKLTY
                     AGETDKYKQHASSNNNDDDADHSNINKDKIEKSDTDNESNWLDQVINN"
     gene            complement(6511..6768)
                     /locus_tag="VHJ57_03395"
     CDS             complement(6511..6768)
                     /locus_tag="VHJ57_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX2487608.1"
                     /translation="MKYRSRTEIVAMILEAANGGATKTKIMYKAFLSYAQLREYLSVL
                     IENNLLEYLDGTQTYKTTEKGLNFLKMHSEIGELLQTPAKF"
     gene            7008..>8949
                     /gene="acs"
                     /locus_tag="VHJ57_03400"
     CDS             7008..>8949
                     /gene="acs"
                     /locus_tag="VHJ57_03400"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX2487609.1"
                     /translation="MSIDKDSSSSDAFFTSPNLKITLEKNKFQAKRAQSLQNIETFWA
                     EQAENLVWNRHWNKTLEWNPPFAKWFTGGLLNASINCLDKHINSEIKNKAAILWEGEN
                     GENITITYYQLYQKVNQFSNALQNLGVRKGDRVTIYLPMVPELPIAILACSRIGAIHN
                     VVFSGFSSTALVDRINDAQSKVIITADGGFRRGKIIELKKIVDEAIINTPSIQNVIVL
                     RRAGNHIEMKDKDIWWHEIIQNQSYYCEPELVESTHPLFILYTSGTTGKPKGVVHSTG
                     GYLTHIYSTAKWVFDFNKQDIFFCTADIGWVTGHSYTVYAPLMHGVTEILYEGTPDYP
                     RPDRYWALVEKYGITILYTTPTALRMYMRYGNEIPNSFNLSTLRLLGTVGESTNPEVW
                     MWYFTTIGKEKCPIIDTWWQTETGGIMLSHCTGIEIMPMKPGTAFFPVPGVDLAVVNE
                     EGNTLPHNQKGYLIIRKPWPGMLMTLWKNDDKYKDKYWKNNNYFAEDYAIIDNDHYFW
                     LLGRSDDVIKVAGHRLGTIELESTFISHLSVAEAAVTSRPDKTKGESIIAFLVLKKDF
                     ADSEQLRKELIDHIRKNIGAIATPSEIYFVNMLPKTRSGKIMRRLLKSIVSDLTIGDT
                     TTLEDDTSIKEIQKIYNDLNDII"
BASE COUNT         3376 a         1198 c         1303 g         3072 t
ORIGIN      
        1 tttatatggt gagttatatt gagcgatgat attttagata atatatttga aaatgcagta
       61 aaaggaaact cgttaatcaa aaataggaaa atcttaacca tagactatat tccacagaaa
      121 ctacaatttc gtgatgaaga gacaactaca atagctcaaa ctttagctgt atctttaaaa
      181 ggggctagac catcaaattt attgctattc ggtaaacctg gaacaggaaa aactgcagta
      241 gttaaaaatg taattaataa gtttaaagaa aaatcaatag accttggggt cgaagttata
      301 gtcgttttta ttaatactaa aatagctaat acagcctata aagttcttta tgaaatcgct
      361 gaagaaatga atataaataa gatagataaa aaaaatcaag tttattttac tggattatcg
      421 cttggggaag caacaaatag aattcttgat tttattaaaa aaagacaatt acatgtaatt
      481 tttgttatgg atgaaatcga ttcacttgtt gacaaaaatg gtgacgatat actttataac
      541 tttactcgag caaatgaaca atttcaagaa gttggtttta tcagtatcat tggaatatca
      601 aatagcttaa catttaagga taaattggat cctagggtga gaagtagctt aagtgaagaa
      661 gagattgtat ttaatccata taaaatccat caattacaaa agatattatc tgaacgagtt
      721 aagttagcct ttcatgatgg tgttgtcaca gaaggaacaa taaatttatg tgcagcaatt
      781 gcaggaaaag ataacggtga cgctagaaaa gcaattgatt tgcttcgcgt agcagctgaa
      841 atagctgaaa tagataagga tactaaagta tacgaaaaat atattcgtct agcacaacat
      901 aaaatagaaa aagataccaa ttttgaaata attaaaaatt ctactactca tactaaaatt
      961 gttttactgg caatagcgaa atcaaaaaac ggaaatactg gagaagttta tgaaatttat
     1021 tcctcattat gtagacatat agatagtgaa tcacttacac aacgtagaat aactcaaatt
     1081 ataaatgaac ttgatcaact cggtttaatt tcgactaata ttgttaacca aggtagatat
     1141 ggtcgctctc aaaaaatcaa gattaccatt cccatttcta cacttaaaga agccttattc
     1201 gatgatcatc tactatctac aataacaaca actattgact aaaatcaatt tgaaatggac
     1261 gtaatgtgga aagattaaca gctattgcta ttcctgaagt aggtattatt cccatagttt
     1321 gttgaaatgg agtttgatct tgccatgttc cagaatttac aatcaatgtt ccacgataat
     1381 tttgtacacc cattacatgt acgtgtccag aatgaaatat gtctggtatt tcagtaatta
     1441 ccatcatatc atccttttct ggagcgattg gtgtcctttg accatatatt ggagataaat
     1501 gtcttgcttt tagtaatgtt ttcattgctt cagcaggatt agagtaactt aatcctggta
     1561 ttgtagcaat aatatcatca agtccttgac catgatacat gagaatttta accccattta
     1621 actcaatata gctaggatta cctaacatag aaaaattttt ccaactgtat aatattcctg
     1681 catccttttt aggtatggca ggttgaggta aagctcttct tccaagatca tgatttcctg
     1741 gaataacaaa tacctgaatc ttttttggaa tctgttctaa tagactagtt aaatgagaca
     1801 tttgttgata tatagtacta tcatttagtt ctttttcttg atttggaaat attccaactc
     1861 catcaattaa atctcctcct atacagagaa agttaatttt tgaaacaatt tcattatcag
     1921 acgaatttaa ccaatttaag aattttaaaa attcttgttc tttaaaatat ttactaccta
     1981 tatgcaaatc agaaattaga accacatatc cgtctgattt tcccttatta ggtatatgat
     2041 caggaatatc aggagaaata acatttttaa taacatgatt tattccttta ttttcaattt
     2101 ctagcattac catttgatcc aatgttaatt ttgatacaag ttttatcgtc tcttcattta
     2161 aagcaattgt atttattatt cctgttaaat catctatagc taattcaata ttattttttt
     2221 tagatcgttt agacattacc agaccagcaa ttattgaagt atttccattt accgaaagat
     2281 tattttctaa ataattatta tttttttctt gtttaatttt tctttttatg gaagatattt
     2341 tattgatacg tttactttct ggtcttgtag ataaaatttt taaagactga tgaaatctac
     2401 ttttaaataa agaaatatag ccattaattc cttcctctgg gtcgatataa tcggcagaat
     2461 cgtaaataat tttaaatgat tctctgtaat ttggtttatt aattgtaatt tctgacgttg
     2521 atataatttc gaattcttga gtttcattta ttgtagtttt tataatattt ttatcttgta
     2581 actttgaatc tgaaacagtt aaagtatcaa attcattaga aatatttatt gtacaaaaat
     2641 ttcgaatatc ttctataaga ataatattag tatcattttg gtttttctta aatctaacaa
     2701 ctttctgaat tatttccatt atatttgaat caaggttttt taaaatttta aatgcttcag
     2761 gatgtatttg atatccgtgt gcagtaatat aagacaatgc atttgatatt tctttctgaa
     2821 aggacaacta caaactatta cgatagaacc atcttattta attacattgc tattataagt
     2881 tcaatacaaa aatattataa agttaaataa tataatataa aaattgaaga aacgtcttta
     2941 ttatttagct gttggtatgt tagtttttct tatttcttat agtattggtg ctaaaataaa
     3001 tattgaaaag gaacaatcgt cattaataaa agaacaattt gaaacaaaaa ttcaaggaat
     3061 agatgaaatt ggtattttcc agaataatgt tctaattgcc ttatccatgt ttattccagg
     3121 tgttggtgta ggaattggaa tattttctgg tgttggtaca ggattagttt ttaatgcaat
     3181 ggctttagaa aatacaacat tgcaaaatat ttctccatta gcaattctta ttactccatt
     3241 tggtataatg gaaatttttt cttatggtct agcaatctca agaagtggaa tgcttatcta
     3301 tgatcttttt aaaaaaaaat cttggaaatt actgcttaaa cccactataa tagaaatagt
     3361 aatagtagta tcaattttaa tagttgcagc attcattgaa tggaatatta taaaacaatt
     3421 tatggaaatt tctaactaat taattattat tatataaata taatcaaagt aatatgataa
     3481 acgatgtaat gatatcaata gagttgattt aaaaatatgg ttaatatcag atgaagcata
     3541 ttcgaacttt aaaatatatt cccggcagct attatagaat tttttttata ttcatcctgt
     3601 aggataggaa caatgattac attacaaaaa aaattgtgat ttagattaat tataatatat
     3661 ttacttttca gataattata aattcaaaat aaaattaata taatataaat agaattgtta
     3721 ggagtaaaaa ttcattataa accaaaattt gataaaaagc caattcttat agcagcatta
     3781 ccagatatgg gaaatgtagc aggattatgc ttaaattttt taataaataa aatagatacg
     3841 aaaattttcg ctgaaatata ttcattttgg ccaccatttg taacatataa aaaaggaatc
     3901 ataaactatt tgcaatcaag ctataagttt tatgaaaatt cacaatataa tataatactt
     3961 tttactggtg attttaatcc tacagatcca agaagattat atgaattatg ttataaagtt
     4021 ttaaaaattg ccgaaatgtt taacgtcaaa cttttatatt ctcttggtgc ctctctaaaa
     4081 ccaattaata caatagaaaa attagtctat gctggagtaa ataaacctga aatggtcaat
     4141 gttttaaatg aattaaattt accgatacta gaagataaag gacagataac aggttttaat
     4201 ggactaattt taggaatagc tcaagaattt aatttagatg gaatctgtct tttaggagaa
     4261 attgataatc caaatgttat tcaacctaaa acttctcaag aaattctaaa agttttatta
     4321 aaaattatta attttccatc aattgattta tcggaattgg aggaagaaga aaaacgaaag
     4381 aagtttatgg agaaacaaat gaattatttc aataaagcag taaatcaaag tgaactacca
     4441 ggaataacat aaatataaac ataaacattt atacaaataa aattaaagta agttaaaatt
     4501 caaatatgtt tcctttttat attgatcaat aaccattacc gacagatcac taaatgtctt
     4561 attttctaaa tccatcattt ttcctctaaa aatattttct ttttccttgt cagtcaagta
     4621 ttcaaaaatc cagacatcga tttctttagt attgactcca ttatttttca aaaataatgc
     4681 aatctcagaa ggcataaaat tttttgataa atcatttggc caaggacgtg gtaataaaat
     4741 aacgctcttc ctatctaata ctgatttgat taattctatc tttttttgtt gaatatcccc
     4801 agttacatga aatgtaacta caaaggcctt atctagtgga acccttgatt ttgaagatgc
     4861 aacttgaata gaactgattc ctggaataat ttcgacattt tcatttccaa aaatctctaa
     4921 taaacgatca acaacctctg attcagaaaa actaacgtcc cctgtaaatg gaacagtaca
     4981 atattcatta tcctgcatat tgccataaac gtattgatat gctgattcct ggtttttcat
     5041 agtaatttca taaatttgtt gcttttcttt atctataaga ttttcaatag ttcttagtgt
     5101 atatctataa cctatgatat attttgatat aaggattttt tctttagcaa tttgagtaat
     5161 ataatcggga tctccaggtc ctacgccaat aacaaacaat ttcttgggca tttgaaattg
     5221 tattacaatt tataatagat aataatatct tttttataaa agaataacat ctattttaaa
     5281 attaaattaa ataatttggt agaaaaagaa actatctgac taaatgatat aactattttg
     5341 acatgcttat cattatatag aataattcac gaatttacat gagcgaactt aagctcgaat
     5401 ttgtaggtaa acaagttaaa gatatgtatg gcacattaat gggaaaagtt gtaggagtta
     5461 ttactgatac tgacggttca attgaatctg ttagtgtaga ttgtggtgat tccggtttaa
     5521 aacaattatc ttatgaacaa ctattggttc aaggtgatta tgtaatcttt attcctaaat
     5581 ggagattaga tgcgcaaaaa ttatttagac aaaaaaattt aacgctcaaa agaattaaag
     5641 cccttcaaca tttagttgaa gaggatgata ctatgaaagt agatgcagaa attgtatatg
     5701 ttaaatatga aaagaaacta gacgaattag aggaaaaaga aaaatctatt aatgaaaaat
     5761 taaaagagag acttgatgag ctaaatgaat caacaaaaaa tattaaatca attttatttg
     5821 atgccaagtt acaatacaaa agccatgaaa tatcagaaga aacatatcta caagttacaa
     5881 gatatactaa agaattaatg gatcatatta atttagaaaa agaagaaatc aataatataa
     5941 aaagtagact tgaagaacaa aacctagaaa acacattacc tttgccacat tcaattcaac
     6001 cagaggagga aaaagataat gaaaaagaaa acacaaattt atctactacc aatacaaagc
     6061 ccgttagtcc tactataact aatactccca atacatcatc atcatcaact gtgactgcat
     6121 caaatacaaa taattctaat tcttcaaata catcattacc tgtatcctct acatcaaatg
     6181 ataatacaca aactaaactc acctatgctg gtgaaactga taaatataaa caacatgcat
     6241 catctaataa taatgatgat gacgcagatc actccaatat aaataaagat aagatagaaa
     6301 aaagtgatac agataacgaa tccaattggc tagatcaagt aataaataac taatttttat
     6361 atatttaatt tttagattct aaaatttaat tattaaattt aattattatt atctacgatt
     6421 ttagtaaaat attagaattt aaagaaagaa ataataaaaa gtaaagaaaa agttagtaaa
     6481 aaaagttagt ggtgaattta tattttatta ttagaatttt gctggggttt gtaataattc
     6541 tccaatttca ctatgcattt taagaaagtt taatcctttt tctgtggttt tataggtctg
     6601 agttccatca agatattcta atagattgtt ctcaatcaaa acagaaagat attctctcaa
     6661 ttgtgcataa cttagaaaag ctttgtacat aatttttgtc ttggttgctc ctccattggc
     6721 tgcctctagt atcattgcta ctatttctgt tctacttctg tatttcacga tcatcataca
     6781 caatatttgt ttattttaat atattgacaa aatgtagtat aacattattc tatactaaca
     6841 ctcaatgtca caattattag gctatcttat tttttaatta acttgttaac taaacttgtt
     6901 tatagatatt atttaataga acatataaat agcttagtca aataacctta acttacattt
     6961 cttataatac aataatttaa ctaggagtac tattattatt atatgtcatg tcaattgata
     7021 aagacagttc atcatctgac gcatttttca caagtcctaa tctgaaaatt acattagaga
     7081 aaaataaatt tcaagctaaa agagcacaat cattacaaaa tattgaaaca ttttgggcag
     7141 aacaagctga aaatttagta tggaatagac attggaataa gactctggag tggaatcctc
     7201 catttgctaa atggtttact ggtggattat taaatgcatc aataaattgc ttagacaaac
     7261 atatcaactc tgaaatcaaa aataaggctg ccatattatg ggaaggagag aatggagaaa
     7321 atataacaat aacatattat cagctttatc aaaaagttaa tcaattttca aatgcattac
     7381 aaaatcttgg tgtaagaaaa ggagatcgag ttacaatcta tttgccgatg gtccccgaac
     7441 ttcctatcgc aattttagct tgttcaagaa ttggagctat tcacaatgtg gttttttctg
     7501 gttttagttc tacagcattg gtggatcgga taaatgatgc tcaatctaag gtcataatta
     7561 ctgctgatgg gggatttaga agaggaaaaa taattgaatt aaaaaaaatt gttgatgaag
     7621 ctattattaa tacaccttcg atacaaaatg ttatagttct tagaagagcc ggaaatcata
     7681 tcgagatgaa agataaagat atttggtggc atgagataat tcaaaatcaa tcatattatt
     7741 gtgaacctga attagtagaa agtacacatc ctctgtttat actttataca tcaggaacaa
     7801 cgggcaagcc aaaaggagtt gttcatagta ctgggggtta tttgacacat atttattcta
     7861 cggcaaaatg ggttttcgat tttaataaac aagacatttt tttctgtact gcggatatag
     7921 gatgggtaac aggtcatagc tatacagttt atgcaccttt aatgcatgga gtaactgaga
     7981 tactatatga aggaacccct gattatccac gtccagatag atattgggca cttgtcgaaa
     8041 aatatggaat aactattttg tatactacac ctacagctct tagaatgtat atgagatatg
     8101 gtaacgaaat cccaaactca tttaatttat caactcttcg attattagga acagtagggg
     8161 aatcaactaa tccagaagta tggatgtggt attttacaac aattggtaag gaaaaatgtc
     8221 ctataattga tacatggtgg caaacagaaa caggtggaat aatgcttagt cattgtacag
     8281 gaatagagat tatgccaatg aaaccaggta cagcgttttt tcctgtgcca ggtgtagatc
     8341 tagccgttgt aaatgaggaa ggaaatactt taccacataa tcaaaaaggc tatttaatta
     8401 ttagaaaacc atggccaggt atgttaatga ctttatggaa gaatgatgat aaatataaag
     8461 acaaatattg gaaaaataat aactattttg ctgaagatta tgctattata gataatgacc
     8521 attatttttg gcttttaggt cgatcagatg atgttattaa agttgctgga catagattag
     8581 gtacgattga attagaaagt acatttatat cgcatttatc ggtagccgag gcagcggtca
     8641 ctagtagacc tgataagaca aaaggtgaat caattatagc attcttagtt ttgaaaaaag
     8701 attttgctga ttcagagcaa ttacgcaaag aattaattga tcatataaga aaaaatattg
     8761 gagctattgc tactccatcc gagatatatt ttgttaatat gctacctaaa accagaagcg
     8821 gcaaaataat gcgaagatta ttaaaatcta tagttagcga tttaactata ggagatacta
     8881 caactctaga agatgacacg tccattaaag aaattcaaaa aatctataac gatttaaatg
     8941 atataataa
//
LOCUS       DATAFW010000147         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5738751, whole genome shotgun sequence.
ACCESSION   DATAFW010000147 DATAFW010000000
VERSION     DATAFW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5738751"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VHJ57_03405"
     CDS             <1..1413
                     /locus_tag="VHJ57_03405"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX2487610.1"
                     /translation="YHQFKKIANIIGSGSNKNKKNILKGLLIALSPLESKYLIKIITG
                     EMRIGSVEGLVEIALSRAFDRELQSIREAMLISGDISQVAVLAKKNILHNVIMKPFVP
                     VSFMLADVMFSAEEIINYYNKPLICEYKYDGIRLQMHKFDKKVRLFSRNLVDITYAFP
                     ELVKAAIESTIKTQDTSIHNQVDFILDGELIAFKNDRPLHFQELQKRLRRKNVTDYIT
                     TEIPIYYIVYDIMYFKDNQVLKQSLLDRKNILSTISFKKPIINSSYKISDSIEQVIAI
                     FNESKDIGHEGLVVKDPLSQYHPGKRGRYWMKLKKELDTIDAVIVIAEYGHGKRAGVL
                     SDYTFAVIDEDKKDTNNNKNNLNLNNNNNNYLENCTLKTIGKAYSGLTDKEIDEMTER
                     LKQIIVEDNGNRILVKPEIVLEVAFDTIQKSDRHNSGFALRFPRIKNIRIDKSLKDID
                     TLEKVRQIYKNQVYVKHMKI"
BASE COUNT          668 a          199 c          253 g          522 t
ORIGIN      
        1 tatcatcaat ttaaaaagat agcaaacata attggttctg gttcaaataa aaataaaaaa
       61 aatatattaa aaggattgct tattgctttg tctcctctcg aatcaaagta tcttatcaaa
      121 attattacag gtgaaatgcg aattggctca gttgaaggtc ttgtagagat tgctttatct
      181 cgtgcttttg atagagaatt acaatctata agagaagcta tgcttatttc aggagatatt
      241 tcacaagtag cagttttagc aaaaaaaaat attcttcaca atgtaataat gaagccattt
      301 gtgccagtaa gttttatgtt agcagatgtt atgtttagtg cggaagagat aataaactat
      361 tacaataaac ctttgatttg tgaatataaa tatgatggaa taaggttaca aatgcataag
      421 tttgataaga aagtacggct tttttctcgc aatctggtag atataacata tgcatttcct
      481 gagttagtga aagctgcaat agaatctaca ataaaaactc aagacacatc aatacataat
      541 caggtagact ttattttgga tggagaacta atagccttta aaaatgatag accattacat
      601 tttcaagagc ttcagaaacg acttagaaga aagaatgtta ctgactatat tactactgaa
      661 attcccatat attacatagt ttatgatata atgtatttta aggataatca agtactaaag
      721 caatcactct tggatagaaa aaatatttta tcaacaatct cttttaaaaa accaataatc
      781 aattcaagtt ataaaatttc agactcaata gaacaagtaa ttgcaatatt taatgaaagt
      841 aaagatattg gacatgaagg attagtagta aaggacccat tatctcaata tcacccagga
      901 aaaagaggaa gatattggat gaagttaaag aaggaactag atacaattga tgcggtaatt
      961 gtgattgctg aatatggtca tggaaaaaga gcaggagtat tatcagatta tacatttgca
     1021 gtaatagatg aagataaaaa agatactaat aataataaaa ataatttgaa tttaaataat
     1081 aacaataata attatcttga aaattgtacg cttaaaacaa tcggtaaagc atactcgggg
     1141 ttgactgata aagagataga tgaaatgact gaaagattaa aacaaatcat agtagaagat
     1201 aatggtaata gaattttagt aaaacctgaa atagttttag aagtagcatt tgatacaata
     1261 caaaaaagtg acaggcataa tagtggattc gcattacgat ttccaaggat aaaaaatatt
     1321 cgtattgaca agagcctaaa ggacatagat actttagaaa aagtcagaca gatttataaa
     1381 aaccaagtat atgtaaaaca catgaaaatt tgaagtataa atttagtaca ttatatcgta
     1441 gtatttttat tatactctaa atttatttag caaattattt tcatccttga tatagtcaaa
     1501 agtattgttt attataatta tattctttgt aattgtaaat ctatattatt ccatgacttc
     1561 ttctatgaac taataattct tcttcactcg acattccata accacaaatt tcacaaaaaa
     1621 caagatttga gatatcagct at
//
LOCUS       DATAFW010000148         4850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_807284, whole genome shotgun sequence.
ACCESSION   DATAFW010000148 DATAFW010000000
VERSION     DATAFW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807284"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /gene="gltX"
                     /locus_tag="VHJ57_03410"
     CDS             complement(<1..1535)
                     /gene="gltX"
                     /locus_tag="VHJ57_03410"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX2487611.1"
                     /translation="MDIDVDTQKLLEATTLRNAIDYNGSARLDTVISKILGSKPEIKN
                     NLKEVIPKIKEIVEQINSLSIERQRELLLQNYPNYLETKKAVEKNEFQLPPLLNAKDG
                     QVVTRFPPEPNGYPHIGHAKAAIIDEEYAKMYSGKLILRFDDTNPLNEKIEYYDAIKQ
                     GLLWLNIAPDIIKNTSDDINLLHYYGRELINKGSAYICLCDQNTIHHLRSKGVPCQCR
                     VQSDKILENSDKIFNGFYHQNEAIIRFKGKMDSHNTAMRDPTLFRIIENTHPLLGDKI
                     ILWPTYDFAAPIEDSLDGVTHALRTKEYELRNELYKQILKSLDMRIPEVIEFSRLEFE
                     GLPVSKRKLKPLIEDKMVEGWSDPRLPTLMGLKRRGFTPEAIRRFVLSLGITLSETKP
                     SIEVLEAFNRKIIDSKSKRLLFVNDPVKINIKNVEHKEVEFQNHPSIEMGTRKIYVKN
                     VVFISRQDAEKLKNGTEIRLMELYNIKVLQIDIDKKEGIVEYTDNILKENIPKIQWVS
                     EED"
     gene            complement(1537..2538)
                     /locus_tag="VHJ57_03415"
     CDS             complement(1537..2538)
                     /locus_tag="VHJ57_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX2487612.1"
                     /translation="MKDTTKVEVENYITKVNNHLVSYLSGKPSELYQASTHYLKSGGK
                     RLRPIMVIKSCEMFGGNQQDALPAASAVEFIHNFSLVHDDIMDNDDQRHGIPTVHKSF
                     GLPLAILSGDILFSKAFQILSTTTVNSIKDSSILSMVRKLSSACVDICEGQAKDIQFS
                     QRQDFPSEEEYMDMISKKTAALFNVSCSLGALSSRNATEIDVNNMSDFGKNSGIAFQL
                     IDDLIGIAGHSKQTGKAVGNDIREGKKTYPILLSIKKASELERSHILEVFGKGHCDTF
                     SLKKAIDVISSLQIEEIVRKKAMDYSEKARKALINYEDSDPKKILQELSNYIVKRSK"
     gene            complement(2510..3619)
                     /gene="fni"
                     /locus_tag="VHJ57_03420"
     CDS             complement(2510..3619)
                     /gene="fni"
                     /locus_tag="VHJ57_03420"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX2487613.1"
                     /translation="MSESNNPEIDLIMSRKRDGIDIPLSNNVQSRFVSNYLEYVKFVH
                     NALPEIDFDEIDTATEFLNYKFGAPIIIDSMTGGTPEALKINSRLGQLAETFSFGMGL
                     GSQRAGLKSSSLAETYSIARKNAPSAFLIANIGGAQLAEGLDIKMIEKIISMIDANAL
                     VIHLNPLQELIQPEGEPKYRGVLKKIEKISENITIPIIVKEVGSGISREVALKLQQAN
                     ISCINIAGSGGTSWAGIEKIRATKVRNDIKEHLGELFWDWGIPTALSLIEVRNAVKLP
                     LIASGGIRNGLEIAKCIALGANMGALAYPFLKAASQSKESLFTFSTRLISELKGAMFL
                     TGSSNIIELAKSRLILTGELADMVINNEGYHQSRS"
     gene            complement(3631..4425)
                     /locus_tag="VHJ57_03425"
     CDS             complement(3631..4425)
                     /locus_tag="VHJ57_03425"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HEX2487614.1"
                     /translation="MKHRLVLIKLGGSVITYKEKPLSANYKAINNISKILSKLEIPLI
                     IVHGGGSYGHYWSVKFDMHTKLQKYPPRGISVVHSSMLTLNNIIIYSLNKHRLNPYGI
                     SPSSFLITGQPNSNKILELKKMTKDNIIPVTYGDAVYIINNKYSIVSGDVLMSMLSKI
                     LNPSKIIFALNVDGLYNNMKEKKIIKEIKFETAKIYKNKQTKINLINDTNIDDVTGGM
                     KRKVEEATKMAETGKDVIFINGLDPRQIQNAIQDKNFKGTIFKGKI"
     gene            complement(4433..>4850)
                     /locus_tag="VHJ57_03430"
     CDS             complement(4433..>4850)
                     /locus_tag="VHJ57_03430"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487615.1"
                     /translation="LKHSTGQLVSIVKRFRENNLPEFHHLSNDVSRICERAVNALEEG
                     NYLHIGKLMNENQTILERIGVSTTEINNIINSSLQYGAVGSKLTGAGGGGCVLSLIHH
                     QDKEYFLASMKKRGYECFPVMIENQGLKVNIKSRNF"
BASE COUNT         1455 a          861 c          682 g         1852 t
ORIGIN      
        1 aaatcctctt ctgataccca ttggattttt ggaatgttct cttttaatat attatctgta
       61 tattctacta taccttcttt tttatcaatg tcaatttgta agactttgat attatataac
      121 tccatcaatc taatttctgt cccatttttc aatttttcag catcctgtct agatataaaa
      181 accacattct ttacgtaaat ttttctggtt cccatttcaa tagatggatg attttgaaat
      241 tctacttctt tgtgctctac attttttata tttattttga ctggatcatt aacaaataat
      301 aatctctttg atttagaatc aattatcttt ctattaaaag cctctagaac ttctattgac
      361 ggtttagttt cggaaagagt tatcccaaga gataatacaa atctccgtat agcctctggc
      421 gtaaaacctc ttcttttcag gcccattaaa gttggaagtc taggatctga ccacccttca
      481 accatcttgt cttcaataag aggttttaat tttcgtttag agacaggcag tccttcaaat
      541 tccaggcgtg aaaattctat tacttctggt attctcatat caagactttt taatatctgt
      601 ttatataatt catttcgcaa ttcatattct tttgttctaa gtgcatgagt aacaccgtcg
      661 agactatcct cgattggtgc agcaaaatca taagtgggcc aaagaattat tttatcacct
      721 aacaatggat gagtgttttc aattattcta aaaagggtag gatctctcat tgctgtatta
      781 tgactatcca tttttccttt gaatctgatt atggcttcat tttgatgata aaaaccatta
      841 aaaattttat cagaattttc taaaatttta tcagattgta ctctgcattg acatgggact
      901 ccttttgacc tcaaatgatg aattgtattt tggtcacata aacaaatata agcacttccc
      961 ttgttaatta attccctacc gtaatagtgt aacaaattaa tatcatccga agtatttttg
     1021 attatatccg gtgctatatt taaccataat aatccttgtt tgatagcgtc ataatattct
     1081 attttctcat ttaacggatt tgtatcatca aaccgtagta ttagttttcc agaatacatt
     1141 ttagcatatt cttcatcaat aatagctgcc ttagcatgac ctatatgagg atacccattt
     1201 ggttcaggag gaaacctagt tacaacttgt ccatcctttg cattaagaag tgggggtaat
     1261 tgaaattcat tcttttcaac ggcctttttt gtttctaaat aattaggata attttgaagt
     1321 aataattctc tctgtctttc tatagataat gaattgattt gttccactat ttccttaatt
     1381 ttaggtatga cctccttgag gttatttttt atttctggtt tagagcctaa aattttggaa
     1441 ataactgtat ctaatcttgc gctaccatta taatcaattg catttcttaa agtagtcgcc
     1501 tctaataatt tttgtgtatc cacgtcaata tccatattat ttactcctct tcactatgta
     1561 attagataat tcctgaagta tttttttagg atcagaatct tcataattga ttagagcttt
     1621 cctggctttt tcactataat ccattgcttt ttttcttaca atttcttcaa tttgaagtga
     1681 agaaattaca tctattgctt tttttaaact aaaagtatca caatgacctt taccaaaaac
     1741 ctccagaata tgagatcttt ctaattctga ggctttcttt attgagagta aaatggggta
     1801 agtttttttc ccttctctaa tatcgtttcc tactgctttt ccagtttgtt tagaatgacc
     1861 tgcaattcca attaaatcat caattagctg aaatgcaatg cctgaatttt taccaaaatc
     1921 gctcatatta ttgacatcta tctctgttgc atttctagac gaaagtgctc ccaacgaaca
     1981 agagacattg aatagagcag ctgttttttt tgaaatcatg tccatatact cttcctcaga
     2041 gggaaaatcc tgtcgttgag aaaattgtat atccttggct tgaccttcac atatgtcaac
     2101 gcatgctgat gaaagttttc ttaccatgct gagtatagaa ctatccttta tagagttaac
     2161 ggtagtagtc gaaagaattt gaaaagcttt tgaaaaaaga atatcaccag ataagattgc
     2221 gagaggcaat ccaaacgatt tatgtacagt tggaattcca tgtctctgat catcattatc
     2281 cattatatca tcatgaacta aggagaaatt atgaataaat tctaccgcgg aagcagctgg
     2341 taaagcatct tgttgattcc ctccaaacat ttcacaggac tttataacca ttattggtct
     2401 aagacgtttt ccaccacttt ttaaataatg ggttgacgct tgatataatt ctgaaggttt
     2461 ccctgataga taagatacaa gatgattatt aactttcgtt atataatttt caacttctac
     2521 tttggtggta tccttcattg ttaataacca tgtctgctaa ttcaccagtt aatataagtc
     2581 ttgatttagc taattctata atatttgatg acccagtgag gaacattgct ccttttaatt
     2641 cagaaattaa acgagtagaa aatgtaaaaa gactttcttt tgattgagaa gccgctttaa
     2701 gaaaaggata tgccaatgca cccatattcg ccccaagtgc tatgcatttt gctatttcaa
     2761 gaccgtttct tattccgcct gatgcaatta aaggtaattt tacagcattc ctaacctcta
     2821 tcaaacttaa agcagtagga atcccccagt cccaaaataa ttcacccaga tgttctttaa
     2881 tatcatttct tactttggta gctctgatct tttctatccc agcccaacta gtaccaccag
     2941 atccggcaat attaatacaa ctgatatttg cttgttgaag ttttaaagct acttctcgcg
     3001 aaatacctga accaacttct ttaactatta ttggaatagt tatgttttcg ctaatttttt
     3061 ctatcttttt caaaacacct ctgtattttg gttcgccttc tggttgtatt aattcctgta
     3121 atggattaag atgtattact aaagcgttag catctatcat cgaaataatt ttttcaatca
     3181 tcttaatatc taatccttct gcaagctgtg cgcctccaat attagcgatt aaaaaagcag
     3241 aaggtgcatt ctttctagct atcgaataag tttctgctaa agaactactt tttagaccag
     3301 ctctttgact tcctaatccc atgccaaaag aaaatgtctc agctaattgt cctaaacgtg
     3361 aattgatttt taaggcttct ggggttccgc ctgtcataga gtcgattata attggagcgc
     3421 caaacttgta atttaagaat tcagtagcag tatctatttc atcaaaatct atctcaggca
     3481 atgcattatg aacaaattta acatattcta aataattaga tacaaaccta gattgaacgt
     3541 tattagaaag aggaatgtct atcccatctc tttttctact cattattagg tcaatttcgg
     3601 gattatttga ctccgacata taatatatct ttatatctta cccttaaaaa tagtaccttt
     3661 gaaattttta tcttgtattg cgttttgaat ctgtctggga tccaaaccat ttataaaaat
     3721 aacatctttc ccagtttcag ccatctttgt ggcctcctcg acttttcttt tcattccccc
     3781 agttacatca tcgatatttg tgtcgttaat tagattaatt tttgtttgtt tatttttata
     3841 gattttagcg gtttcaaatt ttatctcttt tataattttt ttttctttca tattattata
     3901 aagaccatct acgttaagtg caaaaataat tttggaagga tttagaattt tcgataacat
     3961 tgacattaaa acatcaccag aaactataga atatttgtta tttattatat agacagcatc
     4021 tccatatgtg actgggatta tattatcttt tgtcattttt tttaattcta agattttgtt
     4081 actatttggt tggcctgtta tcaaaaatga tgaaggagat atgccatagg gatttaagcg
     4141 gtgtttattt aatgaatata ttattatgtt gtttagtgtt aacatagatg aatgaacaac
     4201 agatattcca cggggaggat atttttgtaa tttagtatgc atatcaaatt ttacagacca
     4261 gtaatgtcca tatgagcctc ctccatgaac tattattaat ggaatttcta atttacttag
     4321 aattttcgaa atattgttga ttgctttata atttgcagac aatggttttt ctttataggt
     4381 gattactgat ccacccagtt ttatcagaac aagcctatgt ttcaattata atttaaaaat
     4441 tcctagattt aatattaact ttcaagcctt gattctctat catgacggga aaacattcgt
     4501 atcccctttt tttcatacta gctaagaaat actccttatc ttgatgatgt ataagtgata
     4561 atacacatcc tccgcctcct gcaccagtta atttagaacc aactgctcca tattgcagac
     4621 tcgaattaat tatattgttt atttcagtag ttgatactcc gatccgctct aaaatagttt
     4681 gattttcatt cattaattta ccaatatgca ggtaatttcc ttcttctaat gcgtttactg
     4741 ctctctcaca aattcgagag acatcatttg ataaatgatg gaattcaggc aaattatttt
     4801 cacgaaatct ttttactatt gatactaatt gtccagtaga atgtttcaag
//
LOCUS       DATAFW010000149         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_983102, whole genome shotgun sequence.
ACCESSION   DATAFW010000149 DATAFW010000000
VERSION     DATAFW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983102"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..352)
                     /locus_tag="VHJ57_03435"
     CDS             complement(14..352)
                     /locus_tag="VHJ57_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX2487616.1"
                     /translation="MGIYIENRPWGKFEKFHENENCTVKLIYVNANSRLSLQYHQHRS
                     EFWKIVKGKAKINIDKKELVVKENENITIPCGVKHRIAALEEDCIILEISYGKFDEND
                     IIRIEDDYNR"
     gene            478..1044
                     /locus_tag="VHJ57_03440"
     CDS             478..1044
                     /locus_tag="VHJ57_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487617.1"
                     /translation="MSEIQHFTEGNLIILYAIHKNSSLQLPNHILERIEHCNRLYQII
                     LKSKPDYNKTIILVVAEPHYSELIKKELINKQINEEIIIIDSDSSKVSHTFDNLKELL
                     KQKINPPFVYFVGSVWLKDVVDSIVKSQFKEYKIQFEGALDHRPVMEVEHDKSLETPK
                     KGKEHFKQVIKNKAFDMLLNYIFPEDHK"
     gene            complement(1118..1825)
                     /locus_tag="VHJ57_03445"
     CDS             complement(1118..1825)
                     /locus_tag="VHJ57_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX2487618.1"
                     /translation="MNRRISSIIGSIFVILLLYFGLTENYKNSILYNIDIFAQENKSP
                     VVKNSKIIEFSGINYADVVNSRDINLNGFTVSVWFNTAMNVTSGDNAFLLNKGGFGSD
                     RPGFNLNYGIWLNNREQVGGGFETSSGEDNYITSQGSYADGVWHNAILTFDEKLHLLK
                     LYMDGLEVGTNTTKTGITPDNTGKQTIRLGANSLAEKGKINGNYTGQLDDIQVWNFAF
                     TKEQVADLFTKESKLAR"
     gene            complement(1925..2245)
                     /locus_tag="VHJ57_03450"
     CDS             complement(1925..2245)
                     /locus_tag="VHJ57_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487619.1"
                     /translation="MKPNSFAMREWHLEHVEKVIVRYVKGLSPTATSFEKRNFKKYST
                     MSNCTKQIEYDKKHGVTEEEVIAVMQKIRNDDSFSELRKNPDSVHRLDELEKQLTSPK
                     KISW"
     gene            complement(2382..>3424)
                     /locus_tag="VHJ57_03455"
     CDS             complement(2382..>3424)
                     /locus_tag="VHJ57_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX2487620.1"
                     /translation="YYQNSSNKLSVLPIVTHFENDAGPYITSSIVYAKDLEKGNQNSS
                     THRLLKLDDKNMAIRMVEGRHLHKCFMYAKDHGEDLKVAIVIGVHPAINIAAAYQASY
                     GIDEMHIANTLLNGNLQLGINKFSQLLIPTMSEIVLEGKILSDKTHEEWMVEMLKTYD
                     FRRQQPIFELENIRFRDNAIFHDILPGYSEHRLLMGLPVESKMLDQVKNVVPSTSQVL
                     LTDGGSNWLTAVIQIKKRVEGESKNALLAAFAAHPSLKIAIVVDQDINPTNSVEVEYA
                     VATRCQADKDLIIIENAKGSSLDPSSDQVNLLTTKLGIDATATLLKVKERFEIAKIPG
                     EDSLKLSEYLSE"
BASE COUNT         1135 a          570 c          432 g         1287 t
ORIGIN      
        1 atcctgggtt tgtctaccta ttatagtcat cttcaattct aattatatca ttttcatcaa
       61 attttccata ggatatttct aatattatac aatcctcttc tagtgctgct atcctatgtt
      121 ttacaccaca aggaattgtt atattttcat tctctttaac tacaagttct tttttatcaa
      181 tattgatctt agcttttcct tttacaattt tccaaaattc gcttctatgt tgatggtatt
      241 ggagactcaa tctagaattt gcattaacat aaatcaattt aacagtacaa ttctcattct
      301 catggaattt ttcaaatttt ccccatgggc gattttctat ataaattccc attattatta
      361 aatcattata tttaatcata taataattac taatgaatat tcaacattca ttcaataagt
      421 tataacgttg tattgggaat gtataaaaga atcattaaaa ttaaaattat taattagatg
      481 tctgaaatac aacattttac tgaaggaaat ttgattattc tttacgctat acataaaaat
      541 tcctctttac aattacctaa tcatattctt gaaagaattg aacattgtaa tagactatat
      601 cagattattt taaaatcaaa accagattat aataaaacta ttatattagt agttgctgaa
      661 cctcattatt ctgaattaat aaaaaaagaa ttgatcaata aacagataaa tgaagaaatt
      721 ataataattg attcagattc aagtaaagta tctcatacgt ttgataatct taaagaattg
      781 cttaaacaaa agattaatcc acctttcgta tattttgtag gttcagtttg gctaaaagat
      841 gtagttgact caattgttaa atcacaattc aaagaataca agattcaatt tgaaggcgca
      901 ttagatcatc gacctgtcat ggaagttgaa cacgataaat cccttgaaac tcctaaaaaa
      961 ggaaaagaac actttaaaca ggtaataaaa aataaagcat tcgatatgtt attaaattat
     1021 attttcccag aagaccacaa ataactatct ttcaaaaata agaattaaaa aagaagttta
     1081 agttagaaat acactagtta ataaaattaa ttaggtttta tcgagctaat ttggattctt
     1141 tagtgaacaa gtccgctact tgttctttag taaaagcaaa attccatact tggatatcat
     1201 ccaattgtcc tgtataatta ccatttatct ttcccttttc agctaaagaa tttgctccca
     1261 atcttatggt ctgttttcct gtattatcag gtgttattcc tgtttttgtt gtgttagtcc
     1321 caacttctag accgtccata tatagcttta acaaatgtaa tttttcgtca aaagttagaa
     1381 ttgcattgtg ccagacgcca tcagcatatg aaccctgaga agtaatataa ttatcctctc
     1441 cacttgaggt ctcaaatccg cctccaactt gttctctatt gttaagccat attccatagt
     1501 taagattaaa tccaggtctg tcacttccaa atcccccttt atttagtaaa aaagcattat
     1561 ctcctgaagt gacattcatt gctgtattaa accatacaga tactgtaaaa ccatttagat
     1621 taatatcacg actatttaca acatctgcat aattaatacc ggaaaattct attatcttgg
     1681 aattcttaac aacaggcgat ttgttctctt gagcaaatat atctatatta tataatatac
     1741 tatttttgta gttttctgtc aatccaaagt ataacaataa aataacaaaa atggaaccta
     1801 taatactgga gattcttcta ttcatttagt gtgttataat ttttgacaat ttaaatattt
     1861 tttcttttta tctcatagaa gaataataat agtaatttaa aaagtcagat ttgtaggtat
     1921 aaaattacca actaattttt tttggagatg taagttgctt ttctaattca tctaatctat
     1981 gaactgaatc tggattctta cgtaattccg aaaaagagtc atcatttcta attttttgca
     2041 tcaccgcaat tacctcttct tctgttacac catgtttttt atcatattct atttgttttg
     2101 tacaattaga catagtacta tattttttaa aattgcgctt ctcaaatgaa gtcgcagttg
     2161 gagataaccc ctttacataa cgaacaatta ctttttcaac atgttctaaa tgccactctc
     2221 tcatagcaaa actattaggt ttcataaata tatccacatc tatttttgcc aaaaaggata
     2281 tttaaattaa tatacaaatt atattgtgga atcattatat ttataatata tatgaagttt
     2341 ttttcatatg ctaatacatt ttcatcaaaa tttttagaaa actattctga taaatattct
     2401 gataatttga ggctgtcttc tccgggtatc tttgcaattt cgaacctttc tttgactttt
     2461 aataacgtag cagttgcatc aattcctaat tttgttgtaa gtaaattcac ttggtcacta
     2521 gaaggatcaa ggctagatcc ttttgcattc tcaataataa tcaggtcttt atctgcttga
     2581 catcttgttg ccacggcata ttctacttct acagaatttg tagggttaat atcttgatca
     2641 acgactattg cgattttaag tgagggatga gcagcaaatg ctgcaagtaa agcatttttt
     2701 gactctcctt ctactctttt ctttatctgt attactgctg ttaaccaatt gcttcctccg
     2761 tctgtcagta acacttgcga tgtacttgga acaacgtttt ttacttgatc tagcatcttt
     2821 gactccacag gtaatcccat caatagacga tgttcagaat aaccaggaag aatatcatgg
     2881 aaaatagcgt tatctctaaa tcttatattt tctaattcaa atataggttg ctgtcttcta
     2941 aaatcatatg tttttagcat ttctaccatc cattcttcat gggttttatc agataatatt
     3001 tttccttcta aaacgatttc tgacattgta ggaataagaa gctgcgaaaa cttgttaata
     3061 cctaattgca gatttccgtt tagtagtgta tttgctatat gcatttcatc tataccatag
     3121 gatgcttggt aagctgctgc tatgttaatt gcaggatgaa ctcctataac aattgcaacc
     3181 ttcaaatctt ccccatgatc tttagcatac ataaaacatt tatgcaaatg tcttccctct
     3241 accattctta ttgccatgtt tttatcatct aattttaaca accgatgggt agaagaattt
     3301 tgatttccct tttccaggtc ttttgcatac acaattgatg aagtaatata tggtccagca
     3361 tcattttcaa aatgtgtaac aataggaaga acagataatt tatttgacga attttgataa
     3421 tatg
//
LOCUS       DATAFW010000150         4125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2394538, whole genome shotgun sequence.
ACCESSION   DATAFW010000150 DATAFW010000000
VERSION     DATAFW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394538"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1679)
                     /locus_tag="VHJ57_03460"
     CDS             complement(201..1679)
                     /locus_tag="VHJ57_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX2487621.1"
                     /translation="MTEVIVAKFGGSTIGQNGDQIPTVIERIKAMRKEDKKIVAVFSA
                     PLMEHEGKVKSMTDIAINIGRKYASGQKVDTNILYKTYLNIAKKYLSQKYFEEFEFHL
                     NKFYNYVTSSLNYVNENKRFIDVTRSRILAYGGEITMSYLMDYIMRSNGLKCDHVDIE
                     KWPIITDDNFEAANFMLEESKEHKKHFLKLLDENEVISIGGFIGKTVDGLETTYERGG
                     SDRTAADIAIILYDEFKVQIDFEKDNIVLSADPKIVKEELESIRYLSYNEARLAGMFG
                     MKILDPIAIKEIDDNNFDIPIVITSLLNPSDITIIEKSLINKKHDFLDNVEYNNNDDS
                     SLIKIVTGKRNCVIVRMESIAASYLIASLEKDKQYHNFLKLSPFKVDDIEVTRLLFLD
                     ADYVKRHERHFKAFYPKVEFAYGKGVVTLIGDMMWKMPKIVSTASRTLGDYDINILNL
                     DAQEETSRILIIVDDIDNNVANAIRAIHLQRYKNDLKITHNN"
     gene            complement(1731..2762)
                     /locus_tag="VHJ57_03465"
     CDS             complement(1731..2762)
                     /locus_tag="VHJ57_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487622.1"
                     /translation="MFFFISPIGLGHAARDVAIIHNLKFKNDNKVNFVTGSAAYTFLK
                     SYGWKAFNLYDPPRFEIESGKLNHSFRWLIKYLLYYKKCQKIATKIIEANFEKLIVSD
                     EDFASISIAEEKNLRRILITDIINTSFTKGITSIIEKKMNKKLCQIIKKCDCVIIPDY
                     GYDTENFRYVGPIVREILESREVLRKKFNFTKKTIVVSVGGTDAGKYLIEKVIDSFRR
                     LKTKLDIDLIVVTGPTLKINPAEDFHLLGYVPNFNEYIYASDLIISLAGRSTMDESIV
                     YNIPGIFIPLKNHYEQEEGAKRLGFEYKDISRLDKLIEEKLSSNSDIRKNNVNNGAKK
                     AAEIISSFI"
     gene            3276..3890
                     /locus_tag="VHJ57_03470"
     CDS             3276..3890
                     /locus_tag="VHJ57_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487623.1"
                     /translation="MSNKKNKNNKNDNNKKKKSYFDITRKKRSKNLKTVILPIIAALV
                     IIAGISIYLFSQNTLSVAGFGPLGSAHEHAAFLVKVNGQNIDFSQPKYQVQSDYIHVE
                     NGDGTTLHRHSTNVTFADFLKSINMDIDRKNNCLVFTNGTKYCDNDDDDNKLRIFING
                     NSTNSISDYILKNDDRLLVMYGNETNEQIAKALDELNKIEIQYT"
     gene            complement(3933..>4125)
                     /locus_tag="VHJ57_03475"
     CDS             complement(3933..>4125)
                     /locus_tag="VHJ57_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487624.1"
                     /translation="VEQLKGYGNVVEIGTDIIRLFTFESNIGELSNLAIKNNISFNIS
                     PITLDDIFVILVGNSIKNN"
BASE COUNT         1397 a          629 c          470 g         1629 t
ORIGIN      
        1 ttcaaatgat cttctaatct aaatattgct agtcctttct gggttttata acatcttatt
       61 ccttcaaata cagcagtacc ataatgcaaa gaatgtgtta aaatatgtat tttagcattt
      121 tgattttcat ataaaaaacc atcaatccaa ataatgtttc catagtccat ttgattttca
      181 ttctttaata ctaataaagt ttaattgttg tgagtaattt ttaaatcatt tttatatctt
      241 tgtaaatgta ttgctcttat agcatttgca acattgttat ctatgtcatc tactattatg
      301 agaattcttg atgtttcctc ttgtgcatcc aaattcagta tatttatatc ataatcacct
      361 aacgttctac ttgcagtaga aacaattttt ggcatcttcc acatcatatc tccaattagc
      421 gtaactacac cttttccata agcaaattcg acttttggat aaaaagcctt aaaatgacgt
      481 tcatgacgct tgacatagtc tgcatcaaga aatagcaatc ttgtaacttc tatatcatca
      541 actttaaacg gtgataattt taagaaatta tggtattgtt tatccttttc aagtgatgca
      601 attaaatatg aagcagcaat actttccatt ctaacaataa cacaatttct ttttcctgtt
      661 acaatcttga ttaatgaact atcatcatta ttattatatt ctacattatc taaaaagtcg
      721 tgttttttat taatcaaaga tttctcaatt atagtaatat cagaaggatt cagtagtgat
      781 gtaattacta tagggatatc aaaattattg tcatcaattt ccttaattgc aataggatct
      841 aaaatcttca taccaaacat accggctaat cttgcctcat tataagataa atatcgtata
      901 gattccaatt cttcttttac tatttttgga tccgcgctta gtacaatgtt atctttttcg
      961 aagtctattt gtactttaaa ttcatcatat aaaattattg caatatctgc tgcagttcta
     1021 tcagatcctc ccctttcata tgttgtttca agtccatcaa ctgtttttcc tatgaatcct
     1081 ccaatagata ttacttcatt ttcgtctagt aatttaagaa aatgtttttt atgttcttta
     1141 gattcctcta acatgaaatt agctgcttca aaattatcat cagtaatgat cggccatttc
     1201 tcaatatcaa catggtcaca ttttaaccca ttacttctca ttatgtaatc cataagatat
     1261 gacatggtaa tctctcctcc atatgcgagt attcttgatc tagtaacatc aataaacctt
     1321 ttattttcat taacataatt taacgaagat gtaacataat tataaaactt attaaggtga
     1381 aattcaaatt cttcaaaata tttttgagat aaatattttt tggctatatt taaatatgtt
     1441 ttatacaaaa tatttgtatc tactttttga ccagaagcat attttctacc aatatttatg
     1501 gcaatatccg tcatagattt gacttttcct tcatgttcca ttaatggtgc agaaaaaaca
     1561 gctacaattt ttttatcttc ttttctcata gcctttattc tttctatcac agtaggtatt
     1621 tgatcaccat tctggccaat agtacttcct ccaaattttg caacaataac ttcagtcata
     1681 tgttatacct atcctattgt aatttttgtt atattattaa tgtgtaacat ctaaataaaa
     1741 cttgatatga tttcagccgc tttttttgct ccattattca cattattttt tctaatatca
     1801 gaatttgagc taagtttttc ttcaattaat ttatcaagtc ttgatatatc tttatattca
     1861 aagcccagcc ttttcgcacc ttcttcttgt tcataatgat ttttaagagg tataaatatt
     1921 cccggaatgt tataaactat ggattcgtcc attgtagatc tacccgctaa tgatattatt
     1981 aggtcagaag cataaatata ttcattaaaa ttaggtacat atcctaacaa atgaaaatct
     2041 tcagctgggt ttattttcag agtaggccca gttacaacaa taaggtctat atctaatttt
     2101 gtctttaatc ttctaaaaga atcaataact ttttctatta gatattttcc agcgtcagtt
     2161 ccaccaacgc ttacaacaat tgttttttta gtaaaattaa attttttacg taatacttct
     2221 ctactttcga gaatttctct tacaattggt ccaacatatc taaaattttc tgtatcatat
     2281 ccataatctg gtattattac gcaatcacat ttctttataa tttggcataa tttcttgttc
     2341 attttttttt ctattattga tgttatacct ttggtaaaac tagtgttaat tatatctgta
     2401 attagaattc ttcttagatt tttttcctct gctatagaaa ttgatgcaaa gtcttcgtca
     2461 cttactatta gtttttcaaa atttgcctct ataatttttg ttgcaatttt ttgacatttt
     2521 ttataatata acaaatactt tattaaccat ctaaaggaat gatttagttt tccagactca
     2581 atttcaaacc taggtggatc gtataaatta aaggctttcc atccataact ttttaaaaat
     2641 gtatatgctg cagatcctgt gacaaaattt actttattat catttttaaa ctttaaatta
     2701 tgaattattg ctacatccct tgctgcatga ccaagaccta tagggcttat aaaaaaaaac
     2761 atttatctat acaattgttt tcttcttttt gtattttact gtttaaaatt aatttctctt
     2821 agttggtttt aaattctaat tttgccttat aaattataat tgctttacta gataaagttt
     2881 atgacttctg acaagtcatg agataaatat tctaatattt gaggcttatg tttagataat
     2941 cttttaaaat atatacatca ggtattgtag atttactctc gtaaaacata aaagaaataa
     3001 tatatgtagt tagacacaaa tattttatag attttaaaga atgattgaaa taaataaaaa
     3061 tcagaataga aattttatat atatatttgc ttagattgat atcctatcaa tatttaattc
     3121 ttaatatata gatcaaatgt aagatatgag actctactac cttataatta atgaaggata
     3181 atactataaa ttcgaattat attcttaaaa atagttattt tcaagtttcc gaaagtgcta
     3241 aataatacaa tattattcaa atcaatcaat ttatcatgtc taataaaaag aataagaaca
     3301 ataaaaatga taataataaa aagaaaaaga gctatttcga tataacaaga aaaaaaagga
     3361 gtaaaaactt aaaaacagtt attcttccaa ttatagctgc acttgttatt attgctggaa
     3421 tttcaattta tttgttttct caaaatactc tatctgttgc aggtttcgga cctttaggct
     3481 ctgcacatga acatgccgca tttttagtta aagtaaatgg ccaaaatata gattttagtc
     3541 aaccaaaata ccaagtacaa tctgattata ttcatgttga gaatggtgat ggcactaccc
     3601 tacatagaca ttcaaccaat gttacttttg cggacttttt aaaaagtatc aacatggata
     3661 tagacaggaa aaataattgt ctggtcttta ctaatggaac aaaatattgc gataatgatg
     3721 atgatgataa caagctaaga atattcatca atggaaattc aactaattct atttcagatt
     3781 atattcttaa gaatgatgat cgtttgttag taatgtatgg aaatgaaacc aatgaacaga
     3841 ttgctaaagc attagatgaa ttaaataaga ttgaaattca gtacacttaa atttttttct
     3901 ttttaactat ccttaatctt tttaatatca atttaattat tttttataga atttcctact
     3961 aaaattacaa atatatcatc aagcgttata ggtgatatat taaaggatat attatttttt
     4021 atagctaggt tagataattc acctatgtta gattcaaagg tgaataatct aataatatct
     4081 gtgcctattt ctactacatt tccataacct ttcaattgtt cgacg
//
LOCUS       DATAFW010000151         7943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2044063, whole genome shotgun sequence.
ACCESSION   DATAFW010000151 DATAFW010000000
VERSION     DATAFW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2044063"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VHJ57_03480"
     CDS             complement(<1..710)
                     /locus_tag="VHJ57_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX2487625.1"
                     /translation="MKFLHRGKVKDVYYPNENNKNTLLFVFSDRISAFDVKMTQDIPK
                     KGEVLCKFAKFWFDNLDVQNHMIKTIDANKMLVEHLSMIPIECVVRGYFYGSLVDRYY
                     NNEIDWKNSLDFKILADSFDPVIASKLSEPIFDPTTKSLEHDIPISDNEIISSKILSK
                     NDLDFLKKKSIELYLKMSNIVNTAGFLMADVKFEFGFNRKGEIVLGDSLGPDEYRLWK
                     KVHYQKGSLQESYDKQIL"
     gene            776..1576
                     /locus_tag="VHJ57_03485"
     CDS             776..1576
                     /locus_tag="VHJ57_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX2487626.1"
                     /translation="MVLDKENSYFKNKKISLNYNAYMFKVYQILVIAYTTGILWLRRN
                     PLSLIFTAITPFSLLFVLFIISDGQYTQFAISGSLVMALVGYGLALGQDITYYKTEYK
                     IQDMFVASPISPLTYMTGLALSEILFGFPALVVLLFLIFYIGVSLLYIPLLIFIIFIV
                     WISTSSMGFFLSSHMLHMKNATQIISFVNVFLAILPPVFYSINKLPEVLQYVSYIAPT
                     THASLMVQYAMGFDTPENWSVVIGISVQLVYAIGFITLAKTHALWRET"
     gene            complement(1605..1679)
                     /locus_tag="VHJ57_03490"
     tRNA            complement(1605..1679)
                     /locus_tag="VHJ57_03490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1642..1644),aa:Thr,seq:tgt)
     gene            1770..2039
                     /gene="purS"
                     /locus_tag="VHJ57_03495"
     CDS             1770..2039
                     /gene="purS"
                     /locus_tag="VHJ57_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX2487627.1"
                     /translation="MGNKEFNVIVIIENKSFINDPEGETIYHDLILKNKYNNIKSVRC
                     AKVLKLIVNAKNPSDARLSVKKMCEDLRIFNPIVSNCQVNTISDK"
     gene            complement(2101..2541)
                     /locus_tag="VHJ57_03500"
     CDS             complement(2101..2541)
                     /locus_tag="VHJ57_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2487628.1"
                     /translation="MIESVRDIMIKDLLTISENETALKAAQLMTEKGVSSIIVLSDDQ
                     PIGIVTEKDFIKKICLKELKLSSVKVGNMMSKIRTSATPDTPIDVAVQRMMNNRIRRL
                     PIVENGKVVGIITVTDLAKHLRTILLLNNTLYSTQLSQSNVENY"
     gene            2666..3358
                     /gene="purQ"
                     /locus_tag="VHJ57_03505"
     CDS             2666..3358
                     /gene="purQ"
                     /locus_tag="VHJ57_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX2487629.1"
                     /translation="MQKIAIPIFPGSNCDRDIYHVLTNLYNIKTEFIWHKKEKIKNYD
                     AIIIPGGFSFGDRLRAGIIAAHSPIIKEVKRLSKDGIPILGICNGFQILVESELLPGS
                     LIKNKSLNFTCKWTDLKVRNTHTPFTSKFKMNELFKIPIANGEGRYILNQDQLKEIKK
                     KDQIVFSYHNDDPNGSLDAIAGVSNDAKNIVGMMPHPERTCQRELKLFGLSNSAMKIF
                     DSLIAYIKKISS"
     gene            3384..5609
                     /gene="purL"
                     /locus_tag="VHJ57_03510"
     CDS             3384..5609
                     /gene="purL"
                     /locus_tag="VHJ57_03510"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX2487630.1"
                     /translation="MEILSHSELQYLKKKLKHEPNFLEKEIVGAQWSEHCSYKSSKKY
                     IKLLSSDAERIIKGPGYDAGIIDVGNGDVITIHIESHNHPSAVEPYGGAATGVGGIIR
                     DIISMGTMPIAILNALRFGHITSPFASKEIKISRRESIELYSHSKWLLKNVVRGIADY
                     GNCIGIPTVGGEVEFDESFTNYCLVDVAAIGFGKKKNLIRNIIQENDIIIIAGSSTGR
                     DGIHGASFASKDLAKEDRSAVQIPDPFMEKLLIEVTKEAIEADILKAVKDLGGGGLSC
                     CLSETSANLNKGFDIYLDKVPVKYRDMEPQEIMISESQERMLYITNEWNFKSFAKILN
                     KYEVPFAIIGRVKNHKNLIIYKNGKTIAKMPSHLIAYAPLLNRNSKKPNYIDNLSVKK
                     IFLKLPYNLNKTLLNMLANPTIALKHWIFQQFDHEIGLRTIVRPGITDCAVIKLANDK
                     FLAATLDGNSKLCYQEPYEGTLSCLSEARANIISSGGNPIGIVDHLQFGNPENPEIFW
                     SFQKSIKAMIDFSSFNKIPIVGGKVSFYNETKIGPIKPSPVIGMLGLIESMKCISKIR
                     PSPGDTLYIVGITGNEMGGSEYFQYLNGVIGGIVPKLDLQIDSINSEIILKINREHLI
                     SSSHDCSKGGLSITLTEMAIDSNCGINIDLDKVPHSCERIDFLLFSETPSRFIIGTEN
                     EEKLEKAFSRYKNLKYSKIGKVMDDGDNITFASKNKKIINLEMEIAKKYYENLTSIME
                     N"
     gene            5704..7140
                     /gene="purF"
                     /locus_tag="VHJ57_03515"
     CDS             5704..7140
                     /gene="purF"
                     /locus_tag="VHJ57_03515"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX2487631.1"
                     /translation="MVTENCGVVGVFSLDQHNVIPFLIDSLRALQHRGQESWGIAIPK
                     KKPFRQLGLVSRYADHFKNIARTYKSNAGIGHVRYSTFGKSTIENAQPLKVKDLCIAH
                     NGTIANVEELANMVGGCTFTPQTMSDTFIAAKRLVEHLSIKMNVTDAIKILKQEMIGS
                     FCFTFMTDNGSVYAARDSKGFRPLVLGFHKETNTYIVASESCAFSALGAYLIRDVEPG
                     ELIKINMNGLQTEKFSSDKPHAHCAFEYTYFAHPVSIMEGVNIYAARKRMGEILGKMF
                     TIPSADLVVPVPDSARPAALGYAQQTGLPFEEGLVKDRHINKGALRSFIEPYQSDRIE
                     INKGILPIKDIIEGKNVVIIDDSIIRGTSSASIIHTLREAGANKIYMIVTYPPVQFPC
                     YAGIDFPSMDELLANHKFVNNGQKLGLNEKISKLIGADKVFYNDTKNLAKAIGIPEEE
                     LCFTCSTGNYSSLGITPKFRTRKEVKGE"
     gene            7196..7909
                     /locus_tag="VHJ57_03520"
     CDS             7196..7909
                     /locus_tag="VHJ57_03520"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX2487632.1"
                     /translation="MISLIKAFAIDIDGTLTENGGGMIHLPAMQNLRTLEKLGFRIIY
                     VTGRSSVEAYMLSVFGGTTKIAVGENGGVVTISPTKHILLGNKEKCMYGYKILTRKLE
                     NVKLKNVFDRMTEVVLERSFDIEVGKQILKDNNLSLDLSDSKYSFHINNIGINKATGL
                     AKALEILRIKPSETVTIGDSETDIPLFQFCAFGIALNHADEIVKSKANYVVSENSGKG
                     LVEALDYIALNFFGKNAIK"
BASE COUNT         2824 a         1149 c         1351 g         2619 t
ORIGIN      
        1 cttaaaattt gtttatcata actttcttga agagatcctt tttgataatg aacctttttc
       61 cataatctat attcatctgg accaagagaa tctcccaaga ctatttctcc tttcctatta
      121 aaaccaaatt caaattttac atctgccatt aaaaaccccg ctgtatttac aatattgctc
      181 attttgagat aaagttctat ggactttttt ttcaaaaaat ctaaatcatt ctttgataaa
      241 attttagatg aaattatttc attatcagat attgggatat catgttcgag ggatttagta
      301 gtaggatcaa atattggctc tgatagtttt gatgcgatta ctggatcaaa actatcagcc
      361 aagattttaa agtctagaga atttttccaa tcaatttcat tgttgtaata tctatcaact
      421 agactaccat agaaataacc tctgacgaca cattcaattg gtatcatgga taggtgttcc
      481 actagcattt tattggcgtc aatagtttta atcatatgat tttggacgtc gagattatcg
      541 aaccaaaact ttgcaaattt acataaaact tcaccttttt ttggaatatc ctgcgtcatc
      601 tttacatcaa atgcagatat tctatcagaa aacacaaata acagggtatt cttattattc
      661 tcatttggat aatatacatc tttgaccttt cctcgatgta agaatttcaa tatttaaaat
      721 agtaccaaaa tatcttttta taattatcga ttataaaaaa tgatcattaa ctaaaatggt
      781 tttagataag gagaacagtt atttcaaaaa taagaaaatt agtttaaatt acaatgcata
      841 tatgttcaaa gtttatcaga tattagttat agcttataca actggaatat tatggctgag
      901 aagaaatcct ctttctctta tttttactgc aattactcct ttttctttac tatttgtttt
      961 atttatcatc agcgatgggc aatatacaca atttgccata agtggaagtt tagtaatggc
     1021 tttagtagga tatggactag cattaggtca ggatatcacc tattataaga cagaatacaa
     1081 aatccaggat atgttcgtgg cctcccctat ctcaccatta acatatatga ctggtctagc
     1141 actctccgaa attttatttg gatttccagc gctagtcgtt ttattatttt taatttttta
     1201 tataggagtt tcgttgttgt atattccact ccttattttt ataatattta tagtgtggat
     1261 ctcgacttct agtatgggtt tcttcttatc ttcacatatg ttacatatga aaaatgcaac
     1321 acagattata tctttcgtga atgttttttt agctatatta ccacctgttt tttattcaat
     1381 caacaaactc ccagaagttc ttcaatatgt ttcttatata gcgcctacaa ctcatgcatc
     1441 cttaatggtt caatacgcta tgggatttga tactccagaa aattggtctg ttgtgattgg
     1501 aatatccgta cagttagtat atgctattgg attcattact ttagcaaaaa ctcatgcatt
     1561 atggagagaa acctagctga tgccgaaatt aattttttaa acataggtca tggtgggatt
     1621 tggacccacg acctaaggtt tacaaaacct tcgctctggc caggctgagc tacatgacca
     1681 taaataccat cgatttttat tttactgtaa ttaattcttt gccaataatt aaaatatatt
     1741 taagtagtta taataatctg ggtatattat tggggaataa agagtttaat gttatcgtta
     1801 taatagaaaa caaatcgttt ataaatgacc cggaaggaga aacaatttat catgatttaa
     1861 ttttaaaaaa taaatataat aatattaaat ctgtaagatg tgcaaaggta ttaaaactaa
     1921 ttgtaaatgc aaaaaaccca agtgacgcaa gattgagtgt aaaaaaaatg tgcgaagatt
     1981 taagaatatt taatcccatt gtaagtaatt gtcaagttaa taccatctct gataaataat
     2041 tactaagaaa aagagatatt tcaaaatatt aaatttaaat ttatgagcaa tattgataat
     2101 ttaataattt tccacattac tctgagataa ttgtgttgaa taaagtgtat tgttcaaaag
     2161 taatatagtg cgtagatgct tggctaaatc agttacagta attatcccta caactttgcc
     2221 attttctaca ataggtagtc ttcttatcct attattcatc attctttgaa cagcaacatc
     2281 tataggagtg tcaggcgttg cagaggttct gattttagac atcatgtttc ctactttcac
     2341 gcttgatagt tttaattctt ttagacaaat ctttttgata aaatcttttt ctgtaacaat
     2401 cccgatgggt tgatcatcag ataaaacaat tatagagctt actccctttt cagtcattaa
     2461 ctgcgctgct tttaacgcag tttcattttc agatattgtg agcagatctt ttatcattat
     2521 atctcttaca gattcaatca tagcaatgaa atgtattata aaattgaaaa ccttataatt
     2581 ctatcaattt aatagtattt ctttaacata cttttttttg acccaaaaat attaatccct
     2641 ataaatgttt ttttagaata ttcgaatgca aaaaattgca attccaatct ttcccggaag
     2701 caattgtgat agggatatat atcatgtctt aacaaattta tacaatataa aaactgagtt
     2761 tatatggcat aaaaaagaaa agataaaaaa ttatgatgca ataattattc cgggtggttt
     2821 ttcttttggt gatagactaa gagcaggaat catcgctgct catagtccta taatcaaaga
     2881 agtgaaaaga ctgagcaaag atggaattcc tatattggga atttgtaatg gattccaaat
     2941 tttagttgaa tctgaattac ttccaggctc tttaattaag aataaatccc ttaattttac
     3001 ttgtaaatgg acagatctga aggttagaaa tacacataca ccttttacat ctaaatttaa
     3061 aatgaatgaa ttatttaaaa ttcctattgc taatggagaa ggaagatata ttttaaatca
     3121 ggatcagtta aaagagatta aaaaaaaaga tcagatagtt tttagttatc ataatgacga
     3181 tcctaatggt tctcttgatg ctattgcggg agttagtaat gatgctaaaa atattgtagg
     3241 aatgatgcct catcctgaaa gaacttgtca acgtgaattg aaattatttg gattgtcaaa
     3301 ttcggctatg aaaatttttg attcattaat tgcatatata aaaaaaataa gctcataaat
     3361 agtggaacta tcatagataa tatttggaaa tcttaagtca ttcagaatta caatatctca
     3421 aaaaaaaatt aaaacatgaa ccaaattttt tagaaaagga aattgtggga gctcaatggt
     3481 cagaacattg ttcatataaa tcttctaaaa aatatatcaa attattatct tctgatgcgg
     3541 aaaggatcat aaaagggcct ggctatgatg caggaatcat tgatgtcggt aatggtgatg
     3601 taatcaccat acacatagaa agtcataatc atccatcagc tgttgaaccc tatggaggtg
     3661 ccgctacagg agtgggaggc ataatacgtg acattatttc tatgggaact atgccaattg
     3721 ctatactaaa tgccttacga tttggacaca taacttcacc attcgcctca aaggaaatca
     3781 aaatatcaag aagagaaagt attgaattat attcacattc taaatggcta ttgaaaaatg
     3841 tggtaagagg aatagctgat tatggaaatt gtataggaat tcctacggta ggaggtgagg
     3901 ttgaatttga tgaatctttc acaaattatt gtttagttga tgttgcagca ataggatttg
     3961 gaaaaaaaaa gaatttgata aggaatatta tccaagaaaa tgatattatc atcatagcag
     4021 gcagtagtac tgggagagat ggcatccatg gtgcatcatt tgcatctaag gatttggcaa
     4081 aagaagatcg atctgccgtt caaatacctg atccttttat ggagaaatta ttaattgaag
     4141 tcactaaaga agctattgaa gctgatattc taaaagctgt gaaagatctg ggaggaggag
     4201 gcttgtcttg ttgcctttcg gaaacttccg ccaatttaaa taaaggtttt gacatttatc
     4261 tggataaagt tcctgttaaa tatcgtgata tggaacctca agaaatcatg atctctgaat
     4321 cacaagaaag aatgttatat atcactaacg aatggaattt taaaagtttt gcaaaaatac
     4381 ttaataaata tgaggttccc ttcgctataa ttgggagagt taaaaatcat aaaaacctta
     4441 taatatataa aaatggaaaa actattgcaa aaatgccttc acatttgatt gcatatgctc
     4501 cattgttgaa tagaaatagt aaaaagccta actatattga taatttgagc gtcaagaaaa
     4561 tctttttgaa gcttccatat aacctgaata aaacccttct aaatatgctg gctaatccta
     4621 ctattgcctt gaagcattgg atatttcagc aatttgatca tgaaatagga ctaagaacta
     4681 ttgtgaggcc aggaataaca gattgtgctg tcataaaatt agcaaatgat aaattcttgg
     4741 cagcaaccct agatggaaat tccaaactat gttatcaaga accttatgaa ggtactttat
     4801 catgtctttc tgaagccaga gcaaatataa tttcctcagg tggaaaccca ataggaatag
     4861 ttgatcatct acaatttgga aatcctgaaa acccagaaat tttttggtca tttcaaaaaa
     4921 gtataaaagc aatgattgat tttagtagtt tcaataaaat tccaatcgtt ggtgggaaag
     4981 tcagttttta taatgaaact aaaattggac ctataaaacc ctcgccagta ataggaatgt
     5041 tgggtcttat cgaaagcatg aaatgtatct cgaaaattcg tccatctcct ggggacacat
     5101 tatatatcgt aggaatcacg ggtaatgaaa tgggtggttc agaatatttc cagtacctaa
     5161 atggagtaat aggggggatt gttccaaaac tagatttaca aattgactcc attaactcag
     5221 agataatttt aaaaattaat agagaacatc taattagttc ttctcacgat tgttcaaagg
     5281 gaggcctctc tataacattg acagagatgg caattgattc taattgtgga atcaacattg
     5341 acttagataa ggtacctcat agctgtgaaa gaatagattt tcttcttttt tctgaaaccc
     5401 cttcgagatt tatcattgga acagaaaatg aagagaaact tgaaaaagca ttttctagat
     5461 ataaaaactt aaaatattca aaaattggca aagtcatgga tgatggagat aatattacat
     5521 ttgcttcaaa aaataaaaaa attataaatt tagaaatgga aatcgcaaaa aaatattatg
     5581 aaaatttaac ctcaattatg gaaaattagt gaattaaatt aagagggatt tattttgtta
     5641 aaggacttaa taacaagaca tatgattaaa atattttgac agcttaatta atggtatatt
     5701 gaaatggtaa cagaaaattg cggtgttgta ggtgtttttt ctttagatca acataatgtc
     5761 attccttttc tcattgattc tttacgggct cttcagcata ggggtcaaga atcatgggga
     5821 atagccatac ctaaaaagaa accattcaga caactaggtc tggtttcgag atatgcggat
     5881 cattttaaga atattgctag aacttacaaa tcgaatgctg gaataggtca tgttaggtat
     5941 tctacttttg gaaagagtac tatagaaaat gctcagcctt taaaagttaa agatttgtgt
     6001 attgctcata atggaacaat tgcaaatgtt gaagaattgg caaatatggt tggaggttgt
     6061 acctttaccc cccaaacaat gagtgatacc tttattgccg ctaaaaggct tgtagagcat
     6121 ctttctatta agatgaatgt aactgatgct ataaaaattt taaaacaaga gatgataggt
     6181 tcgttttgtt tcacctttat gaccgacaat ggatctgttt atgctgcaag agattcaaaa
     6241 ggttttagac cattggtttt gggatttcac aaagagacaa atacatatat tgttgcatcg
     6301 gaatcctgtg ccttttctgc actgggggct tatttaataa gagacgtaga accaggagaa
     6361 ttaattaaaa taaatatgaa tggtttacaa actgaaaaat tttcttccga taaacctcat
     6421 gcccattgtg cgtttgaata tacatacttt gcccatccag taagtataat ggaaggtgtg
     6481 aatatatatg cggctagaaa aaggatggga gaaattttgg gtaagatgtt cacaattcct
     6541 tcagcagact tggtcgttcc agttcctgat tccgcaaggc cagcagcatt agggtatgcg
     6601 caacaaacag gattaccttt tgaagaagga ttagttaagg atagacatat caataaaggt
     6661 gcattaagga gttttatcga accatatcaa tctgatagaa tcgaaataaa taaaggaata
     6721 cttcctatca aagacattat tgaaggtaaa aatgtggtaa taatcgacga cagtataatt
     6781 cgaggtacca gctccgcatc tattattcat accctgagag aagcaggagc caacaaaatc
     6841 tatatgatag ttacttatcc tcctgttcag tttccttgtt acgcaggcat agattttcct
     6901 tctatggatg aactattagc aaatcataaa tttgtaaata atggacaaaa actaggactc
     6961 aatgaaaaaa taagtaaatt aattggtgcg gataaagtat tctataacga cacaaaaaat
     7021 cttgctaaag caatagggat accagaagaa gaactatgtt ttacctgttc aactggaaat
     7081 tatagttctc tagggataac accaaagttt agaacaagaa aagaagtaaa aggagaatga
     7141 aagtttctct aaatattttt ttgaaaggat aagattgaaa acgtaaaatt ctactatgat
     7201 ctcattaatt aaagcttttg caattgatat tgatggtact ttgactgaga atggtggagg
     7261 aatgatacat cttcctgcta tgcaaaatct tagaacatta gaaaaattag gttttagaat
     7321 tatctatgta acaggccgat cttctgtcga agcctacatg ttatctgtat ttggagggac
     7381 gactaaaata gcagtggggg aaaatggagg cgttgttact atcagtccga ctaaacatat
     7441 tcttctggga aacaaggaga aatgtatgta tggatataaa atcttaacaa gaaaattgga
     7501 aaatgttaaa ttaaaaaatg tttttgatcg catgacagag gtcgtattag agaggtcttt
     7561 cgacattgaa gtaggaaagc aaatccttaa agataataac cttagtttag atctaagtga
     7621 tagcaaatat tcctttcata ttaacaatat aggaatcaat aaggcgactg gtctggcaaa
     7681 agcattagaa attttgagaa taaaaccttc tgaaacagta acaattggag atagtgaaac
     7741 tgatattcct ttgtttcaat tttgtgcttt tggcattgca ctcaatcatg cggacgaaat
     7801 tgtaaaatct aaggctaatt atgtcgtctc tgaaaattca ggaaaaggct tagtggaagc
     7861 attagattat attgcgttaa atttttttgg taaaaatgct atcaaatgac cctaaaagaa
     7921 gtaatcaacc aaattaggca act
//
LOCUS       DATAFW010000152         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_3278849, whole genome shotgun sequence.
ACCESSION   DATAFW010000152 DATAFW010000000
VERSION     DATAFW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3278849"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VHJ57_03525"
     CDS             complement(<1..476)
                     /locus_tag="VHJ57_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487633.1"
                     /translation="MNFKNTVLFSGVAVLLSFLILNSVTYSFSFAATEYEHKTKWGTK
                     GVAIGQFSQTGGITADSENNIYVADFTGRSNMIQKFSGNGSFILGFGVLGNGPQFFTN
                     PSGLTADSENNIYIADFGNPTYAIKKFSKDGNFIDTFGSFGLGDGEFITPGGLGVD"
     gene            complement(587..1132)
                     /locus_tag="VHJ57_03530"
     CDS             complement(587..1132)
                     /locus_tag="VHJ57_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487634.1"
                     /translation="MKNISKKNIEISYIMLFSVILASGLLLYSFNVDVMGIPPGIVES
                     DYLFGRTIGIVEDENGKPTWIISGTWKTNLSNQTQTTSNSTVFDASFEMIKPDGTSKH
                     THTITNFALEEISNQKNNVTVFNGTGTISMPQEPVTEVPISIQVMNNVLGVIYIDPKK
                     IDNHFGTDDPIYGLPFEESEK"
     gene            1394..>1687
                     /locus_tag="VHJ57_03535"
     CDS             1394..>1687
                     /locus_tag="VHJ57_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487635.1"
                     /translation="MSSNVNIPKLPAKNKSELTEQVFQAIVDSGETGIFQTELCKSFS
                     IDSRDGSRLVGNLEKQSLISRERMLYKGRWTYKLIVKKIPQLKEANRRPIEIAS"
BASE COUNT          610 a          262 c          224 g          591 t
ORIGIN      
        1 ttatctacac ctagtcctcc tggtgtgata aattctccat caccaaggcc aaatgaacca
       61 aaggtatcta taaagtttcc atccttgcta aattttttaa tagcataagt aggattccca
      121 aaatcagcaa tatagatatt gttttcagag tcagcagtta aaccagaagg attagtaaaa
      181 aattgtggac cattacctag tacaccgaaa ccaagaataa atgatccatt ccctgaaaat
      241 ttctgaatca tatttgaacg acctgtgaaa tccgcaacat agatattgtt ttcagagtca
      301 gcagtaatac cacctgtttg actaaattgg cctatggcaa caccttttgt gccccatttt
      361 gttttgtgtt catattctgt tgctgcaaat gaaaaagaat atgtcacact attcaaaatc
      421 aaaaatgata acaaaacagc aacaccacta aaaagaacag tatttttaaa attcatactt
      481 acttaaaaaa atagattata tattaactat tcctataaaa ttgttataat atttgtgggt
      541 tctaaaaatt agaaattatt tttactataa taattaatat ttctttttat ttttcgctct
      601 cttcgaaagg tagaccgtat attggatcat cagtaccaaa atgattatct attttctttg
      661 gatcaatgta tatcacccca agaacgttat tcattacttg aatgcttata ggaacttcag
      721 ttaccggttc ttgaggcata cttattgttc ctgttccatt aaatactgtg acattatttt
      781 tttgatttga tatctcctct aatgcaaaat tagtaatagt gtgtgtatgc tttgatgttc
      841 catcaggttt tatcatttca aaagatgcat caaaaacagt ggaattactt gtagtttgag
      901 tttgatttga taaattagtt ttccaagtgc cagatattat ccaagttggt ttcccatttt
      961 catcttctac gatgccaata gttcttccaa acagataatc actttcaact ataccaggag
     1021 gaattcccat tacatctaca ttaaacgaat ataacaacaa tccgctcgct agaattacag
     1081 agaaaagcat aatatagctt atttcgatat tcttttttga tatatttttc aataaattat
     1141 tgtagttata ttgttcttat tattttttcg tttttatttc tatattatca catttatcaa
     1201 acaaataata tttacttcaa aggtatggaa ttatatcaat tgtgaaaaat aataatattt
     1261 ttttaaacct gcaattatta tgaccattct agttatcaaa caaattaact ggctatacta
     1321 ttaaatatat taaatttaca taagcaaatt cattagattt ttatatcaaa taaactaaaa
     1381 agtatattat cttttgtctt caaatgtaaa cataccaaaa ttacctgcaa aaaataaaag
     1441 tgaattaaca gaacaagtgt ttcaagctat agtagatagt ggagaaacag gaatatttca
     1501 aacagaactt tgcaaatcgt tctctataga tagtagagac gggtcaagat tggttggaaa
     1561 tcttgaaaaa caatctctta tttctagaga aagaatgtta tacaaaggca ggtggactta
     1621 taaattaata gtaaaaaaaa tacctcaatt aaaagaagca aataggagac caattgagat
     1681 tgctagt
//
LOCUS       DATAFW010000153         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6276511, whole genome shotgun sequence.
ACCESSION   DATAFW010000153 DATAFW010000000
VERSION     DATAFW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6276511"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /gene="serS"
                     /locus_tag="VHJ57_03540"
     CDS             <1..876
                     /gene="serS"
                     /locus_tag="VHJ57_03540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX2487636.1"
                     /translation="DHTIRNHIDIGESLDLIDIEKAAKISGSRFYFLKNDLVKLNHAV
                     ISFALDFISEKGYTLLQPPYMIKKNAMEGAVILQDFKDVIYKIQDEDLFLIGTSEHAM
                     AAMHMDQILEGSKLPLRYAGFSPCFRKEAGAHGKDMKGIFRVHHFDKVEQFVYSRPEN
                     SWQEHERMLNIAEEFYKKLGIPYRVVLLCSADLGKISSKTYDIEAWIPAQKNYREIVS
                     CSNCIDYQARRLRIRFRDKPNEETKLVYTLNSTLVATQRTLVAILENYQTTNGVMIPE
                     ILQSYMGGIQEIKSK"
     gene            complement(1001..1453)
                     /locus_tag="VHJ57_03545"
     CDS             complement(1001..1453)
                     /locus_tag="VHJ57_03545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX2487637.1"
                     /translation="MINNTDNYRCIFCSIINGTSEGITVYYNNHFLVLMDKYPISNGH
                     TLVIPKKHYDNLLSMPQEEVGKLYSLVSIIAKAVVSAVSADGFNIGQNNGKSANQIIP
                     HVHVHIIPRFKNDNSYGKWPTRYIAKEDELYDFSKKIKNQLNILSILD"
     gene            1577..1765
                     /locus_tag="VHJ57_03550"
     CDS             1577..1765
                     /locus_tag="VHJ57_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487638.1"
                     /translation="MTETIEIENFNVLFSFDCPKCEYHNEEISVADLTNGSAECGMCE
                     TIFKFEGIDTLKITATIE"
     gene            1841..3541
                     /gene="ilvD"
                     /locus_tag="VHJ57_03555"
     CDS             1841..3541
                     /gene="ilvD"
                     /locus_tag="VHJ57_03555"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX2487639.1"
                     /translation="MKLPSRKTLEGPSRAPHRAMYKAMGLKDTDLEKPIIGISSTCNE
                     ATPCNIHLGKLAQFAKKGVSDVNCTPREFTAIAVSDGIAMGHEGMKASLVSREIIADS
                     IEIMMRAHQYDGLVGISGCDKSLPGTLIGMARLNLPSIFVYGGTIMPGNWHNKDVTIQ
                     DVYEAVGSFDAGKISMEELIQLENVACPSAGSCGGMYTANTMASISEALGISLPGSAS
                     PPAESEKRNEMCYETGKTIATLLENDISPKDVMTFEAFENAITIANALGGSTNAVLHL
                     LAIAKEIGIKLILKDFERIRKRTPHIVNMRPGGLYVMLDLDKIGGVPIVLKTLLKNGL
                     INGKTLTVTGKTMEQNLESFNLNSNVDKNILSPYEKPIEKEGTIKILKGSLAPQGAVV
                     KIAGVKLSKFVGKAKVFETEENAFRAISKNRIVEGDVVVIRYEGPKGGPGMREMLAVT
                     AALVGQGLGEKVSMVTDGRFSGATRGFMIGHVSPEAMVGGPISLVKDGDLINIDVNKG
                     IIDLLISKAEFRRREKKWKPIKPHYKTGVLAKYASLVNSASEGAITRPIIIAHDKKGH
                     "
BASE COUNT         1329 a          473 c          651 g         1095 t
ORIGIN      
        1 gatcatacaa taaggaacca tattgacatt ggagaaagtc tggacttaat tgatattgaa
       61 aaagccgcca agatatcagg atcacgattt tattttttaa aaaatgattt agtaaaacta
      121 aatcatgcag taataagttt tgctcttgat tttatttctg agaaaggata tacattatta
      181 caaccaccat atatgatcaa aaaaaatgca atggaaggag cagtaatttt acaagatttt
      241 aaagatgtaa tatataaaat tcaagatgaa gatctctttc tgattggaac ttctgaacat
      301 gctatggctg caatgcacat ggatcaaata ttagaaggct ccaaattacc tctaagatat
      361 gcaggattta gtccatgttt tagaaaagaa gcaggagcac atggcaaaga tatgaaaggg
      421 attttccggg ttcaccattt tgataaggta gaacaatttg tttattctag accagaaaat
      481 tcttggcaag aacatgaaag aatgctgaat attgcagaag agttttataa aaaacttgga
      541 ataccatata gagtagttct attatgttct gctgatttag gcaaaatatc atctaagaca
      601 tatgatattg aggcatggat accagcacag aaaaattatc gagagatagt ttcatgttca
      661 aattgcattg attatcaggc tagaagatta agaataaggt ttagagataa accaaatgaa
      721 gagactaaat tagtctatac attaaatagc acacttgtag caactcaaag aacgctagta
      781 gcaattttag aaaactatca gacaactaat ggagtcatga tcccagagat tttgcaaagc
      841 tatatgggag gaattcaaga aattaaatca aaataaactt ctatgtatat tttatttaga
      901 taaagataat tacaataata aagacaatta aaattatctt ttgaaggtaa ataaaaataa
      961 agaatgaata ataataataa taataataat ttattattaa ctaatctaga atagacaaaa
     1021 tatttaattg attttttatc tttttagaga aatcatataa ttcgtcttct tttgcaatat
     1081 atctggtagg ccatttccca taagaattat catttttaaa tctaggaata atatgcacat
     1141 gtacatgagg tataatctga tttgctgatt ttccattatt ttgtcctata ttaaatccat
     1201 ctgcagaaac tgctgaaact actgctttgg ctataatact aacaagagag tataatttcc
     1261 ctacctcttc ctgaggcatt gaaagtaaat tatcataatg tttctttgga atcaccaatg
     1321 tatgtccatt actaattgga tatttatcca tcaatactaa aaaatgattg ttataataaa
     1381 ccgttattcc ttcactagtt ccattaatta ttgaacagaa tatacaacga taattatcag
     1441 tattgttaat cataataact ttttctatca ttactaatat tacttttatt tgttgatatt
     1501 ctaaatacag attttactta agtataaaaa ttatagccaa ttttaaatat aactatttcc
     1561 tcctatctct tacataatga cagaaactat tgagatagag aattttaatg tacttttctc
     1621 atttgattgt cctaaatgcg aataccataa tgaggaaata tccgttgctg atttgactaa
     1681 tggaagtgcg gagtgtggaa tgtgtgaaac tatctttaag tttgaaggaa ttgacacatt
     1741 aaaaattacc gccactatag aataaacaat ttaagctcta tcttatataa tactgatgaa
     1801 ggaaagacta ttaaaataat acttttgtat tcttatttga atgaaattac ccagtagaaa
     1861 aactcttgaa ggaccatcta gggcgcctca tagagcaatg tataaagcaa tgggattaaa
     1921 agacacagat ttggaaaaac ctattattgg tatatcttct acttgcaatg aagccacacc
     1981 atgtaatata cacctaggta aacttgcaca atttgcaaag aagggagtat cagatgtgaa
     2041 ttgtactcca agagagttta ctgctatcgc agtatctgat ggtatagcta tggggcatga
     2101 aggtatgaaa gcatctttag ttagtagaga aataatagca gattcgatag aaattatgat
     2161 gagagcacat caatacgatg gtttagtggg aatttcaggt tgtgataaga gtttgccagg
     2221 tacattaatt ggcatggcta gattaaattt accttcaatt tttgtttatg gagggacaat
     2281 aatgcctgga aattggcaca ataaggatgt aacaatacaa gacgtttatg aagctgtggg
     2341 ttcgtttgat gcaggcaaaa tcagtatgga agaactaatt caactagaaa atgttgcttg
     2401 tccatctgct ggctcttgtg gtggaatgta tactgcaaat actatggcat ctattagcga
     2461 agctttaggt atatcgttgc ctggtagcgc ttctcctcca gcagagagcg aaaagagaaa
     2521 tgaaatgtgt tatgaaacag gaaagactat tgcaactctt ttagaaaatg atattagtcc
     2581 aaaagatgta atgacttttg aagcatttga aaatgcaata actattgcaa atgcattagg
     2641 aggatcgaca aatgcagtat tacatctatt agcaatagct aaagaaatag ggataaaatt
     2701 aattttaaag gatttcgagc gaatacgaaa acgtactcct catattgtga atatgagacc
     2761 tggaggatta tatgttatgt tagatttgga caaaattggt ggggtaccaa ttgtattaaa
     2821 aacactatta aaaaatgggc taataaatgg aaagacatta acagttaccg ggaaaacaat
     2881 ggaacaaaac ttggagtcat ttaacttaaa ttcaaatgtt gataaaaata ttttgagtcc
     2941 ttatgaaaaa cccattgaga aagaaggaac aataaagatt ttaaagggat ctctagcacc
     3001 acagggtgct gtagtaaaga ttgccggagt aaagttatcc aagtttgtag gtaaagcaaa
     3061 agtatttgaa actgaagaga atgcatttag ggcaatatca aaaaatagaa ttgttgaagg
     3121 tgatgtagta gtaataagat atgaaggtcc aaaaggcggc ccaggtatga gagaaatgct
     3181 agctgttact gcagcattag taggacaggg ccttggagaa aaagtatcta tggtaactga
     3241 tggaagattc tcaggtgcaa ccagaggatt tatgatagga catgtatctc ccgaggccat
     3301 ggttggaggt cctattagtt tggtaaaaga tggtgatctc attaatattg atgtcaataa
     3361 aggaattatt gatctcttga tatcaaaagc tgaattcaga agaagggaaa aaaaatggaa
     3421 gcctataaaa ccacattata aaactggtgt attggcaaaa tatgcatctt tagtaaattc
     3481 tgcgtcggaa ggagctataa caagaccaat aataattgct catgataaaa aaggtcatta
     3541 atgaaatt
//
LOCUS       DATAFW010000154         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5748413, whole genome shotgun sequence.
ACCESSION   DATAFW010000154 DATAFW010000000
VERSION     DATAFW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5748413"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..773
                     /locus_tag="VHJ57_03560"
     CDS             387..773
                     /locus_tag="VHJ57_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487640.1"
                     /translation="MPHIVLTNVSDFESFYHNFKKEVLIIKNEEKDQTTSTIIRFEDI
                     LLNQLKNLLLIKTIVIENGNQSQTYYIMVMKKNIDQITIRLDPLTDPKNKTDAVKISL
                     ANIAKQYKKIDPEISIGRTNLEQYLL"
     gene            complement(920..2293)
                     /locus_tag="VHJ57_03565"
     CDS             complement(920..2293)
                     /locus_tag="VHJ57_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX2487641.1"
                     /translation="MYNNKNKKQQNLRVLVTGATGFIGSKIVKRLLADRKYNVRCMTR
                     NPDSIYELFNLSGDIIDVVKADASNYLELVEALKEVDVAFYLIHSMEGSSKKWKEFSK
                     RDRIAAENFAKAASITNTKRIIYLSGLIDTDHKDLSEHMKSRKEVGDILRTSKADVTI
                     FRAAVILGQGGGSFQMLQYLVERLPMMVCPKWVLTKSQPISVDDVVTYLVKSIETKDT
                     ASQDYDIGGPEILSYIDMMKRYSSILKKSLKIIIIPFLTPRLSSYWIDLITPVKASLA
                     RPLIDSLKHEAVAHNNEIQKLIPIRLMNFEESIRTAMKEDVRRTKVIKKQRTSDSLNR
                     TILFFSLISLGIIGATYYFLDNRSDLFTPLWIILAILWYFGILFALFFVKNGARLGSM
                     IAGIIGWTTLAFWGFNNIYLISDNFIDFSSQNVAISIRNIIGAIVAGLVIAASHNLFH
                     KIRVLGK"
     gene            complement(2545..>2861)
                     /locus_tag="VHJ57_03570"
     CDS             complement(2545..>2861)
                     /locus_tag="VHJ57_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487642.1"
                     /translation="FWNKLFPYNFKRGITYGIISGISLWLVLFLPLATFIIQPKLDAF
                     ALSAPNQYIFGIANHFQGLYPFVAIGSLVFHTIYGIILGFMIWRMIDLKIFVPINREP
                     IQ"
BASE COUNT         1056 a          422 c          368 g         1015 t
ORIGIN      
        1 ccggaattag aggatacact tagattccta ctcggaaaaa tggaaataag ataaaataaa
       61 agttgtaaca aacacaaaca accctttgca ctatttgcaa ttaaaaacta atttttctaa
      121 tcctttattt tctatagatc caaaatctcc caaaattcat gagttaattg aaatactaaa
      181 aaagataaaa tagtcttttt tcaggaatag tcttttatgt aattgtagtt taattaggca
      241 ctagattttt taaaatgctg aataactaaa atacatgaaa aataaaattt cataatatat
      301 tggtcaaaaa agcaattatc attggggcaa gcattagtgg attatgcatt cctaaaatat
      361 tgtcattttt agaggaatcg tgaaaaatgc ctcacatagt attaactaat gtttctgatt
      421 ttgagagttt ttatcataat tttaaaaaag aagttcttat tattaagaat gaggagaaag
      481 atcaaacaac aagcaccata atcagatttg aagatatcct tttaaatcaa ttaaagaacc
      541 ttttattaat taaaaccata gtcatagaaa atggtaatca gtctcaaaca tattatatta
      601 tggtaatgaa aaagaatatt gatcaaataa ctataagact tgacccatta actgatccaa
      661 aaaataaaac tgatgctgta aaaatatcac tggcaaatat tgctaaacaa tataaaaaaa
      721 ttgacccaga aatctcaatt ggtagaacaa atttagaaca atatctgcta taatagattt
      781 taaaaaattt tctaaaaatt atgtaaacga tgggtcaata aagacatgga taaataatat
      841 taaaatcaag aaattacaaa atacagttta ttagaaaggt tatacaataa atgttcatat
      901 atctaagaaa ttattttatt tattttccta gtactctaat tttatgaaag agattatgtg
      961 aagcagctat aactaatcca gctacaatag ctccaataat atttcgaatt gaaatcgcaa
     1021 cattttgaga tgagaaatca ataaaattgt cagatattaa atatatgtta ttgaatcccc
     1081 agaatgctaa tgttgtccat cctataatac ctgctatcat tgatcctaat ctagcaccat
     1141 ttttaacaaa aaataatgca aataaaattc caaaatacca taaaattgct aatattatcc
     1201 aaagaggagt aaataaatca cttctattat ctaaaaaata atatgttgct ccaattatcc
     1261 ctaaagaaat caatgaaaaa aataatattg ttctgttgag agagtcagat gttctttgtt
     1321 tttttattac cttagttctt ctgacgtctt ctttcattgc agttctaata gattcttcaa
     1381 agttcattag tctaatagga attaactttt ggatttcatt attatgagct actgcttcat
     1441 gttttaaact gtctattaat ggtctagcaa gtgatgcttt tacaggagta ataagatcaa
     1501 tccaataaga ggaaagtcta ggagtcaaaa atggaataat aataattttt aatgattttt
     1561 ttagaatact tgaatatctt ttcatcatat ctatatagct aagaatctca ggtccgccta
     1621 tatcataatc ttgactagct gtatccttgg tttctattga cttaactaaa taagttacta
     1681 catcatctac tgatataggt tgtgatttag tcaataccca tttaggacat accatcatcg
     1741 gaagcctttc cactagatat tgtaacattt gaaaggagcc acctccttga cctaaaataa
     1801 cagcagcacg aaatattgta acgtcggcct ttgaagtcct taaaatatct cctacttctt
     1861 ttctactttt catatgctca gataaatcct tatgatctgt atcaataaga ccactcaggt
     1921 aaattattct ttttgtattt gttattgagg ctgctttagc aaaattttca gcagcaattc
     1981 tatctctttt agagaattct ttccattttt ttgatgaacc ttccatagaa tgtataagat
     2041 aaaaagccac atccacctct tttagagctt ctactaattc taaataattt gaagcatcag
     2101 cttttacaac atctatgata tcaccactta aattaaaaag ttcataaata gaatcgggat
     2161 ttcttgtcat acatctaaca ttatattttc tatcggctaa aagtctctta acaattttac
     2221 tgccaataaa tccagttgct ccagttacta ggactcttaa attttgttgt tttttatttt
     2281 tgttattgta catatacagt tcttatagtt tgttttcaaa atagaatttc attttataaa
     2341 aaagtaacga tagtttttta acattcctaa ttagtcctca aagtgctaaa atatatttat
     2401 ttattttcat ttcacaacga tactagttaa atattcgatc tttattttta ttacaataga
     2461 tataaaaatg gtgaactagt ttcctgggct ttcatgcttg ttgcaacaag gattttcata
     2521 tttctgtatc caaatcttta aagattattg tattggttct ctgttgattg gtacaaatat
     2581 ttttaaatcg atcattctcc atatcatgaa tcccaatatg atcccatata ttgtatgaaa
     2641 tactaaagat cctatagcga caaatggata aagtccttgg aagtgattag ctattccaaa
     2701 tatatattga tttggagcag ataatgcaaa agcatctagt ttgggttgaa ttataaatgt
     2761 agctaggggt aggaataata ctagccatag agaaataccc gatattattc cataagttat
     2821 tcctcgtttg aaattataag gaaatagttt attccaaaat a
//
LOCUS       DATAFW010000155         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_286510, whole genome shotgun sequence.
ACCESSION   DATAFW010000155 DATAFW010000000
VERSION     DATAFW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286510"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /gene="cofE"
                     /locus_tag="VHJ57_03575"
     CDS             complement(<1..608)
                     /gene="cofE"
                     /locus_tag="VHJ57_03575"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEX2487643.1"
                     /translation="MFKKIEIIPIYLNINIDKKAKLLNILLKSIKNNNQIIKNGDIIV
                     IAQKIISKNEGRSVNLNNIIPSSKSIELGRKINKDPRIVELILQESRKIIRVFENIII
                     TETHHGFICANAGIDQSNVSKSKNRVLLLPRDPDKSADTIRKEIYEKTRKNIAILITD
                     TFGRPFRMGQTNIAIGIAGINALKNYKGKPDMFGKIMKVTEIA"
     gene            complement(608..1273)
                     /gene="serB"
                     /locus_tag="VHJ57_03580"
     CDS             complement(608..1273)
                     /gene="serB"
                     /locus_tag="VHJ57_03580"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX2487644.1"
                     /translation="MLIIFDVEGVLLNAEYLPILAQVFGPSKEKEIWDITKQGIRGDI
                     DWEEGLKKRVNALKGIKFDDAKQIAQNLEIMPGAKDLCLALKDAGWKMIAVSGGFTII
                     TDRLKKELGLDLIFSNVLIFKNGLLDDLDLKVTSDKSLVVRPIIKDWGLKKEEIVVVV
                     DGANDIKLFDIAGYTVGFCPVELVKNKADDVVELRNLLLLKNLLEKRFGKCILKTNLK
                     NKL"
     gene            1525..>1721
                     /locus_tag="VHJ57_03585"
     CDS             1525..>1721
                     /locus_tag="VHJ57_03585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX2487645.1"
                     /translation="MIKSLRQSPKLGQIKNIKINFPRVSIIVPARNEETYLRKCVDSL
                     VNQDYPDYEIILVNDESSDKTL"
BASE COUNT          568 a          276 c          203 g          674 t
ORIGIN      
        1 gctatttctg taactttcat aattttacca aacatatctg gttttccttt ataatttttt
       61 aaagcattaa ttcctgcaat accaattgca atatttgtct gacccattct aaaaggacga
      121 ccaaaggtat cagttattaa gatggcaata ttttttcttg tcttttcata tatttctttt
      181 cttattgtat cagcagattt atcagggtcc cttggtagta aaagtactcg atttttactt
      241 ttgcttacat tactttgatc tataccagca tttgcacaaa taaaaccatg gtgtgtttct
      301 gtaataataa tattttcaaa tactcgaata atttttcgag attcctgaag aattaattct
      361 acaattcgtg ggtccttatt gatttttctg cctaattcta tagatttaga ggaaggaata
      421 atattattga ggttaactga tcgtccttca ttttttgaaa taattttttg agctatgact
      481 attatatctc cattttttat aatttgattg ttatttttta tggattttaa aagaatattt
      541 aataattttg ctttcttatc aatgtttata tttagatata tgggaattat ttcaattttt
      601 ttaaacatta gagtttattt tttaggttag tttttaaaat acatttaccg aatctttttt
      661 ctaataaatt ctttaataat aataagttcc ttaattcaac tacatcatct gctttatttt
      721 tgactaattc aacgggacaa aaaccaaccg tgtaacctgc tatgtcaaat aattttatgt
      781 cattagcccc atcaacaact accacgattt cttctttttt caaaccccaa tcttttatta
      841 taggtctaac aacaagtgat ttatctgaag tgacttttaa gtcaagatca tctaataatc
      901 catttttgaa aattaaaaca ttagaaaaaa ttaaatctaa tcctaattct ttctttaatc
      961 gatcagtgat aattgtaaaa ccacctgaaa cagcaatcat tttccatcca gcatctttta
     1021 gtgctaaaca aagatctttt gctccaggca taatttctag attttgagct atttgtttgg
     1081 catcgtcaaa ttttatacct tttaaggcat taactctttt tttcaggcct tcctcccaat
     1141 ctatgtctcc tcttattcct tgctttgtaa tatcccaaat ttccttttct ttggaaggtc
     1201 caaaaacttg agctaaaatg ggtaaatatt ctgcattaag taatactcct tccacgtcaa
     1261 atattattaa catagatatt tgcttaagcg actatctaac cgtatattaa gcttgtctga
     1321 tcaaataaaa aaattaagta tcaaaaccga aatcaaacgg acaaacagaa aactaattat
     1381 taaaattagg ctagtaaaaa ataataacat ggaatttgat ttttaactaa tcatacaatg
     1441 gaaaatgcta caataatttt ttattttttc attattttta ttacagcagg aattttttct
     1501 atttggattt acctattatt ctttatgatt aaatcactta ggcaatcccc aaagttgggg
     1561 caaataaaaa atatcaaaat caactttcca cgggttagta taatagttcc tgccagaaat
     1621 gaggaaacat atcttcgtaa atgtgtagac agcttagtaa accaagatta tcctgactat
     1681 gaaataattt tagttaatga tgaatcatct gacaaaactc t
//
LOCUS       DATAFW010000156         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_638494, whole genome shotgun sequence.
ACCESSION   DATAFW010000156 DATAFW010000000
VERSION     DATAFW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638494"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VHJ57_03590"
     CDS             complement(<1..245)
                     /locus_tag="VHJ57_03590"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX2487646.1"
                     /translation="MSSQADQQKQTSNALQPGATAPNFTLRTTPDKSVSLDQFRGNPV
                     ILLFYPADFSPVCGDEVSLYNEILPEFQRLNAEVIGIS"
     gene            complement(249..416)
                     /locus_tag="VHJ57_03595"
     CDS             complement(249..416)
                     /locus_tag="VHJ57_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487647.1"
                     /translation="MSCQKKYNERLLHLIETGRIDPTKVISYKMKLDDVPKGYGGFDK
                     KKDIMKIVLKS"
     gene            complement(583..834)
                     /locus_tag="VHJ57_03600"
     CDS             complement(583..834)
                     /locus_tag="VHJ57_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487648.1"
                     /translation="MRLNKTKDLGAEIINFDDEDPVDVIKKETNGKGAICIDAVGFEA
                     VGHMAGNVSDDNGRLIKNNSLKGSNNKTVTKVPIMIILR"
     gene            complement(1028..1165)
                     /locus_tag="VHJ57_03605"
     CDS             complement(1028..1165)
                     /locus_tag="VHJ57_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487649.1"
                     /translation="MIIENAEVCYYLMMIQKCQIAGGIFLDAIGPLSKIMKDEQAQAD
                     S"
     gene            complement(1763..1921)
                     /locus_tag="VHJ57_03610"
     CDS             complement(1763..1921)
                     /locus_tag="VHJ57_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487650.1"
                     /translation="MGTNIIKKEKVNEDINYKYKCEECGINFGYSLGDMELHKLTVHL
                     QKGDVEIK"
     gene            2063..3517
                     /locus_tag="VHJ57_03615"
     CDS             2063..3517
                     /locus_tag="VHJ57_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HEX2487651.1"
                     /translation="MERLNKFIEYTQTEVDKGINPGGYYKAIWSRDAAFILKDQFLSG
                     HFKVALQQIVLIWSNQIIATERSLSLLYCNLKIKPLLYGRGSPEMDFNPIIADDNTKK
                     KFDGALPTTIYYERGFCEVYGQNPDIDSIALMISTTSWILSNLLDLKDNPKTEPVITL
                     SLNERVDNRENEIPFSKFSLTDIINFLIPCMLKAILYLQNRDIDNDGLLEQKHNEDWM
                     DTILRIGKVVYSQACWILALKNFSILLRKIGNKKESKRLKDMTNRAILAVEDKMWSAK
                     DGCYIDLLDADLHLDKKMHNRLVTQDISLYLVALTEDSINTNSIHVDSINRREYSKSE
                     SYTNNINLDTCKRALSTLEALKKRAWKNNLPLVTETEITKTGPWLLKTNEYHNHTFWP
                     WITGIEILARTRFGKMDDCSFLLSKFVSDNVVHSNMLYEWINPLTFQGSGAYPFRTGI
                     SSLRIAAIESLKENIKYNYNQIIENHLGCTNTSK"
     gene            3771..4232
                     /locus_tag="VHJ57_03620"
     CDS             3771..4232
                     /locus_tag="VHJ57_03620"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX2487652.1"
                     /translation="MTKVKYEIDVNAPVEQVYGYYTNPDNIKEAWPQDIVKESQSLSG
                     SKNEEGSEMKVKGEYMGRKDEMILQVTDKEPNKRLITEQKDGPFKYWKSVQEFNESQN
                     TTHISHSIDYELPSSGKIANFLSGDSANNRIEKRLQQAAQTVKQKLESQPT"
BASE COUNT         1591 a          651 c          659 g         1497 t
ORIGIN      
        1 gagattccaa taacctcggc attgaggcgt tggaattcag gtaaaatttc attatataaa
       61 gatacttcat ctccacagac aggcgaaaag tcggctgggt aaaataaaag aattactgga
      121 tttcccctaa attgatccaa agatacagac ttatctggag tagtacgtaa agtaaagttt
      181 ggtgcagtag caccaggttg aagtgcatta gatgtttgtt tttgttgatc tgcttggcta
      241 gacatatcct atgattttag aacaatcttc attatatctt ttttcttatc aaagccacca
      301 tatcctttag gtacatcatc aagtttcatt ttatagctta taaccttggt tgggtctatt
      361 cgacctgttt caatgaggtg aagtaagcgc tcattatatt ttttttgaca ggacattgtc
      421 ccatggtaat ctgaatgtta cggttaaaca acattcctag tgaaaattga tcatagcctg
      481 atccatatac tactggtatt gatatagtag aatatttttt tgcaacttat gccatccaag
      541 taattaattg caagggatta actggattat attcagggtt agttatctta gtatgatcat
      601 gattggtact tttgttactg ttttattatt actgcctttt aggctgttat ttttaattag
      661 tctaccgtta tcatcactaa catttccagc catatgtcct acagcctcaa atcccacagc
      721 atctatacaa atggcccctt ttccattagt ttcttttttt attacatcaa ctggatcctc
      781 atcatcaaaa ttaataatct cagcgcctaa atcttttgtc ttgtttaaac gcataggcca
      841 atgatcaact gagaaaactc atgcagctcc tcttaaaaag gaaactcata actggaaaat
      901 atccaacagg acatgctcca aatactgcga catcatcccc ttgctgcaca tttgcgatgt
      961 cagctgcaaa gtacccagtg ggaatatatc gctaagtaat agtacttgtt catcagtttt
     1021 aatcccttca ggaatcagct tgagcctgtt catctttcat aatttttgat aatggtccta
     1081 ttgcatcgag aaaaattcca ccagcaattt ggcatttttg tatcatcata agataataac
     1141 atacttcagc attctctata atcatataaa agaattaaaa ttctaatttg ttcctatact
     1201 tagataataa atttggttac gatcttaaaa tttgaattta ttaaatcttt aatcttaatt
     1261 gatgaacatg gatgaacatc actattatta tatgggtaga acatatcaag ttgataaatt
     1321 taatgaccaa tgatgatatt atatgatata attatgattt gaataatcta ctgcatatat
     1381 ttgcaattat aatattatcc ataatttaga gaattagtta aacaaacgaa ttttttaaat
     1441 cattctttaa tctagtttct tttttttgta gtaaaattag aagataaaaa taaatctaaa
     1501 tttgattcag aagcacaaaa taattcctta ttttgttatt tgagcaataa tcaaatattt
     1561 ttactaaatc agattcagtt agtagtacga atttatttag ttttgatcat atattttaaa
     1621 ttttaaaaga agactctaac aaatacagat ctattatgca agttgatttt tatcgaggcg
     1681 ttttttaaat tagaattaat tgattaatta tgctgcctgc acctattaaa aaatgatgca
     1741 gatgtaaatc tattattaat agttatttta tttccacatc tcctttttgt aaatgtacag
     1801 ttaatttatg tagttccata tctcctaaac tatatccaaa gttaattcca cactcttcac
     1861 atttgtattt ataatttata tcctcattta ctttttcttt ctttatgatg tttgtaccca
     1921 ttgcttttgt acctcattta caaaagttac ttgataaatg ttcatatccc tatatctaat
     1981 attttttcga aaactattta atagtttaac tcgctgatcg ggctttatag tgaatctttt
     2041 agctcatgag aaaaagatct ttgtggaacg tttgaataaa tttattgagt atacacaaac
     2101 agaagtagac aaaggtatca atcctggtgg ctattacaag gcaatatggt caagggatgc
     2161 agcttttatc ctgaaagatc aatttctatc aggccatttt aaagtagcac ttcaacaaat
     2221 tgttttaatt tggtctaatc aaataattgc aacagaacgc tctctatctc tcctgtattg
     2281 taatttgaaa ataaaaccgt tattgtatgg aagaggttct ccagagatgg attttaatcc
     2341 aattattgca gatgataata ctaaaaagaa atttgatggt gctcttccta ctacaatata
     2401 ttatgagaga ggtttttgcg aagtttatgg acaaaatcca gatattgact ctatagcatt
     2461 aatgatatcc actacatcat ggattctatc taatctgctt gatttgaaag ataatcctaa
     2521 aaccgagcct gtaattacat tatcactgaa tgaaagagtt gataatagag agaatgaaat
     2581 tcctttctca aagttttcgt tgaccgatat cattaacttt ttgattccat gtatgctaaa
     2641 ggcaatacta tatctacaaa atagggatat agataatgat ggattattag aacagaaaca
     2701 taatgaagat tggatggata caattctacg aatcggaaaa gtagtttata gtcaagcctg
     2761 ttggattctt gcattaaaaa atttttcaat attattgaga aagataggaa acaaaaaaga
     2821 atcaaaaagg ctaaaggata tgaccaacag agcaatattg gcagtagaag acaagatgtg
     2881 gtctgcaaaa gatggatgtt atattgatct tcttgacgct gatttgcatt tagataagaa
     2941 gatgcataat agacttgtca cacaagatat ttctctatat cttgttgcat taacagaaga
     3001 tagcataaat acaaacagta tacatgtaga tagcattaat aggagagaat attccaaatc
     3061 tgaaagttat actaataata ttaatttaga tacatgtaaa cgtgcattaa gtactctgga
     3121 agcattaaag aaaagagctt ggaaaaataa tttacctttg gtaactgaaa cagaaattac
     3181 aaagacaggt ccatggcttc ttaaaacaaa tgaatatcat aatcatactt tttggccttg
     3241 gatcacagga atagaaatac ttgcacgaac taggtttggc aagatggatg attgcagttt
     3301 tttgctgtca aaatttgttt cagacaatgt tgtacattca aatatgttat atgaatggat
     3361 aaaccctttg acttttcaag ggagtggtgc atacccattt aggacaggaa tatcctctct
     3421 acggatagct gcaattgagt ctcttaaaga gaacatcaaa tacaactata accagataat
     3481 cgaaaatcat ttaggttgca caaatacaag taaataaaaa acaattataa ataaaaatta
     3541 gtatatacga atttctaaaa ctttttgttc tttttctaat aacaattaac caaagaatat
     3601 ttaaattgat ataattgaga attataaata tattggttat taataagaag aattgatatt
     3661 attaaggtaa acacatctcc atcagatcga aacttgaaga tattcttata gtgcccaacg
     3721 gccatctcaa ttttattaac ctttataaat tatatttcat agaaaacatc atgacaaaag
     3781 tcaaatatga aatagacgta aatgctccag tagaacaagt atatggatat tataccaatc
     3841 cagataacat aaaggaagca tggccacagg atattgttaa ggaatcacaa tcactttcag
     3901 gttcaaagaa tgaagaaggg tctgaaatga aagtaaaagg agaatatatg ggaagaaaag
     3961 atgagatgat tttacaagta actgataaag aaccaaataa aagactaata acagaacaaa
     4021 aggatggacc atttaaatat tggaaaagtg tacaagaatt taatgaaagt caaaatacta
     4081 cacatataag tcattctatt gattatgaat tacctagttc aggaaaaatt gcaaattttc
     4141 tttcaggaga tagtgctaac aataggatag aaaagagatt acaacaagca gctcaaacag
     4201 taaaacaaaa attggaatca caacctacct aatcaggatt caaataaaaa tctaacctca
     4261 taacatccta tatattttcg ttattaaata tttcgtaacc attacttatg atagtatttt
     4321 agttattatt tttattattc taaaaggaag taatatatgt atatttaata acgtataatt
     4381 tacttaatta cgtttaaa
//
LOCUS       DATAFW010000157         6840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6280036, whole genome shotgun sequence.
ACCESSION   DATAFW010000157 DATAFW010000000
VERSION     DATAFW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6280036"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VHJ57_03625"
     CDS             <1..322
                     /locus_tag="VHJ57_03625"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487653.1"
                     /translation="KTFSFKNKIVEQWNLGVLIASNNSNYTEAKSIAESIFKINFGKN
                     LITRRNDNNNLFLKGRCADIIYKSKTIGFIGEVTPDLITKFNLHVPLSAFEFNLSPVL
                     KVEC"
     gene            complement(1678..1854)
                     /locus_tag="VHJ57_03630"
     CDS             complement(1678..1854)
                     /locus_tag="VHJ57_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487654.1"
                     /translation="MISRQKEAEIISEILLRAASEPDFRNLLIKNSAEILEKYNISNE
                     AKYIIKKSIIDLTQ"
     gene            1951..3255
                     /locus_tag="VHJ57_03635"
     CDS             1951..3255
                     /locus_tag="VHJ57_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEX2487655.1"
                     /translation="MIFQSQKKWTLNGTSRVIPVEKTLMHVSEISRKIGLTRIADITY
                     MDKLYIPNYSCVLPGTEDYIWVYSGKGPTKQHAKVSAIMESIERYSALPTNYSRKFIT
                     GTFNQLKHSYNLLHPDEVVEPLTFDFQNDMLMDYVEGYDIINKENILVPAALALFRYT
                     PNSPSLNPFAFHHTNGLASGNVVEEAVCHALCEVIERDAISLAQLRASAIPFHILYKI
                     YQNLRIEGYHINTISKEHFLDDNSLYPDVKISEDNFFPVTKLINKFKKFNIELIIKDI
                     TTNIKIPTFNVACVEWISHDYGYLAEGHGTHPDKRIAILRAITEVSQTRAANIQGARD
                     DLRKIHYNYNNTDDKNAWQFMSSQKTINFSDIITYQNEDILDDINLIIKFFKEVGLNR
                     AIIVNLTNPKINVPVVRAIVPGLETFKISKSVMGFRARRSFK"
     gene            3252..3908
                     /locus_tag="VHJ57_03640"
     CDS             3252..3908
                     /locus_tag="VHJ57_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HEX2487656.1"
                     /translation="MNLPIIYLGPSLEINKAKKIIDADFKPPAKKGDFINLNLLTERR
                     DIVLIDGVFLQEYPPSPIEVFQVIKNKNFKVFGASSLGALRAVELENFGMQGIGKIFE
                     LFKKNIINSDDEIAVTFDSNYNLLSEAMIDIRYNIFLCWKKGIIDEETKLFMINLAKR
                     IYFPFRSYENIIKKSLHFFPSKEKYISRFSNYIFINKVSLKELDAIRALEYISSLPKT
                     "
     gene            3985..4371
                     /locus_tag="VHJ57_03645"
     CDS             3985..4371
                     /locus_tag="VHJ57_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487657.1"
                     /translation="MTENLSKSNAQYKTNCKKILELSPKIRYVGVTNKFGRTIAGQLR
                     IGLTPFLKTEEMRNELFVHSMIYNIRKNYEKSIGETEFIFISNKNVDIISFYIDELVY
                     YITIDKNVDKNELYTLIEQTKDLAIS"
     gene            complement(4398..4853)
                     /locus_tag="VHJ57_03650"
     CDS             complement(4398..4853)
                     /locus_tag="VHJ57_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HEX2487658.1"
                     /translation="MYRQLASTWTKMWKTNSEELKSRAVGWRKELSVHRINKPSRLDR
                     ARRLGYKAKQGIVVIRARVGRGGMRKQRPVAGRRPKHSGVVHIKQATNMRLVAERRVN
                     EKYPNMEIIGSYFLYKDGKHSWYEVILADPSHPSIYKDKEMRGKLKSLT"
     gene            complement(4958..5428)
                     /locus_tag="VHJ57_03655"
     CDS             complement(4958..5428)
                     /locus_tag="VHJ57_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX2487659.1"
                     /translation="MISIDEKLYSLGFELPSPPKSSGSYIPLVEVNGLIFVSGQIPLD
                     TGSNPVTIKYKGKIGKDTTLEEGQNAAILCTLNALSLLKDHLGSLDKIKKIVKVSAYI
                     NCVESFSDHPTVINSASNLLETLFGEKGKHSRIAIGVDSLPLGSTIEIDFIVSI"
     gene            5524..5886
                     /locus_tag="VHJ57_03660"
     CDS             5524..5886
                     /locus_tag="VHJ57_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487660.1"
                     /translation="MSLAIASVITYFVYLGLMDSMTRSSPNYLSISNDTAVSIIFKNE
                     NISSDHLKTVNSEYVYIKGNGEIYESKKDTNFIGNYVRKNSPTITTGNHFGWEINLPA
                     LNQTYYVDHLTGEIISTN"
     gene            5962..>6840
                     /locus_tag="VHJ57_03665"
     CDS             5962..>6840
                     /locus_tag="VHJ57_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX2487661.1"
                     /translation="MQLTGNVLICDPISKIGITILEKAGLNVVDKPSITNSELLSEVE
                     NYDVIVVRSRTKITSEVISNAKKAKIVARVGVGLDNIDVESALQNNIEVVNSPESAIN
                     AVAELVIGLMLSLARNLPLADCEIKKGNWLKKELNGIELRGKYLGIVGVGNIGRNLAR
                     IARSLRMNIIGYDTIPIQQDFIKEVGMITTDFNTLIASSDFISCHVPSTPHTRHMFNE
                     SSFSTMKPTAFFINASRGEVVDENALYNALTTKKIAGAALDVFEVEPPTNKKLLQLSN
                     IICTPHIGSQTKESQEL"
BASE COUNT         2500 a         1009 c         1017 g         2314 t
ORIGIN      
        1 aaaaacattt agttttaaaa ataaaatagt agaacaatgg aatttaggcg ttctaattgc
       61 atctaacaat tctaactata cagaagctaa atcgattgca gaatctattt ttaaaataaa
      121 ttttggtaaa aatcttataa ctaggagaaa tgataataat aatttattct taaaagggag
      181 atgtgctgat ataatatata aaagtaaaac aataggcttt ataggagaag tcaccccaga
      241 tttgattaca aaattcaatt tacacgttcc tttatcagcc tttgaattca atttatcacc
      301 agtattaaag gttgaatgtt aaaatatact caattaagtt caggagtaaa aattataaca
      361 atgtactctc aatttctact ctatgggtat tagatttctt gaaggaatag aacatcaact
      421 attattgtaa tgtacttttg tatttgtatt ttcttggtct ctgcttacgc gatagataca
      481 ttaaagacaa tagtagtcct tagagacagg aaaagaaatg acatgtctcc atagaattag
      541 attcagagat atgcctcata tcaaatctat aaaagaagaa aaagagtaat agcccatacc
      601 atacaggaaa caattataca gagtggaact aacaaacatt tctagtaata gatctttatt
      661 gaacctgaac acagctttct gcttggaatt cttatcttaa aggagagaca cattacttgt
      721 tactgagcat tttaggagat caatgtttca aaagtacgga aagcatactg tcattataaa
      781 cagatggtgg gggaatatgg taatcacaaa catgcaacct tctgcatcta aaacatagat
      841 taaattcacc actagaaaag agttcaattg aaaagataat tcaatctttc aaggatagga
      901 cggaaagttt tgatgattat tatgtatatg ttaaaaagga atataatcag tttcataaga
      961 attggacaca attcttttat ctatgtacaa tgatactaaa gctaccaatc gtgaattcaa
     1021 aatctggtta taggaaaaaa tatatcctaa caaaacccgt ataccgaatt atttaaagat
     1081 ttttaacaaa actcattaaa aactaaaaat aaagaataga aatactcaaa gatctgagct
     1141 atttatatac tgaatttttc gacgttaggt actatgacgg ttaccagagc aactattaga
     1201 gtttagatac ctactatcta tgcatacacg gaatacaaat gcaatttatt taatgtacat
     1261 agtttgatac aatacttatt tttgtaaaat gatacaatat ccaaaagaag aattattcca
     1321 ttagttgttt gaaagaagaa ggtaaataaa tctcagccat aaaataagtt gcatttttat
     1381 atattactgt attttgttat aaaaaaacaa gataattaaa aaaagtctct tttttagtca
     1441 tatattaaac cataattgtt ttataatggc ttatatttac cttaattacc gaatatttag
     1501 ctgaaatatc cggtaacccc gttatagaga gtgcactagg agaaatacac ctttgtgtat
     1561 ggctatatcg atgaatagtt gtgagtcaga ggcgggggac aacatcaatt ttttatattg
     1621 gacttcgaat aaaacttggc aaatcaagaa taataatttg aattaataga tcgaccatta
     1681 ctgggttaaa tcaattatac ttttcttaat aatatatttt gcttcattag aaatattata
     1741 tttttccagt atttccgctg aatttttaat aagtaaattt ctaaagtcag gttcgcttgc
     1801 agccctgagc agaatttcac tgattatttc tgcttctttc tgtcgcgaaa tcaatgtaaa
     1861 caaaaaaaac aatgaagtct tatataatct taatgttata atgttgaatt agtaaaattg
     1921 ataagaactt taataaataa tttttaaata ttgatatttc aaagtcaaaa aaaatggact
     1981 cttaatggta catccagagt tattccagta gaaaaaacac ttatgcatgt ttcagaaata
     2041 agtagaaaaa ttgggcttac tagaattgcc gatattacat atatggataa attatacata
     2101 cctaattatt catgtgtttt acccggtact gaagattata tttgggtata tagtggaaaa
     2161 ggtcctacga aacaacatgc aaaagtaagt gcaattatgg aaagcattga acgatattct
     2221 gccttaccta ccaactattc taggaaattt attactggaa cttttaatca actcaaacat
     2281 tcgtataatc ttctacatcc tgacgaggtg gttgaaccat taacttttga ttttcaaaat
     2341 gatatgttaa tggattatgt agaaggctat gatattatta ataaggaaaa tatcttggtt
     2401 cctgcagcat tagcattatt tagatatact ccaaactcac cttctctaaa tccttttgca
     2461 tttcatcata ctaatggact cgcatccggc aacgttgttg aggaagcggt atgtcatgca
     2521 ttatgcgaag ttattgaaag agatgcaata agcttagctc aattacgggc aagtgcaatt
     2581 ccattccata ttttgtataa aatatatcaa aatttaagaa tagaaggata tcatattaac
     2641 acaatttcca aagaacactt tcttgatgac aattctctat acccagatgt taaaatttct
     2701 gaagacaatt ttttcccagt aactaaatta ataaataaat tcaaaaaatt taatattgaa
     2761 ttgattatta aggatatcac tacaaatatt aaaattccta cctttaacgt tgcatgtgtt
     2821 gagtggatat ctcatgacta tgggtatttg gctgagggac atggtactca tccagataaa
     2881 agaattgcaa tattaagagc tataaccgaa gtttcccaaa caagagcagc aaacattcaa
     2941 ggtgccaggg atgatttaag gaaaatacat tataattata ataatacaga tgataaaaat
     3001 gcatggcaat ttatgtcttc acaaaagaca atcaattttt ctgatataat aacttatcaa
     3061 aatgaagata ttttagatga tattaatctt attataaaat tttttaaaga agtaggatta
     3121 aacagggcta taatagtcaa tttaacaaat ccaaaaataa atgttccagt tgtgagagct
     3181 atagttcctg gtcttgagac atttaaaata tcgaagtctg ttatgggctt tagagctagg
     3241 agatccttta aatgaattta ccaattatat atttggggcc ctctttagaa ataaacaaag
     3301 caaaaaaaat tattgacgct gactttaaac ctccagccaa aaaaggtgac tttataaatt
     3361 taaatctatt aacggaaaga agagatattg ttctaataga tggagttttc cttcaggagt
     3421 atcccccttc tcctattgaa gtttttcaag ttattaagaa taaaaacttt aaagtttttg
     3481 gcgcctctag tcttggagca ttacgcgcag tagaattaga aaattttgga atgcaaggta
     3541 ttgggaaaat ctttgagtta tttaaaaaga atattatcaa ttcagatgat gaaattgcag
     3601 taacatttga ttccaattat aatctgcttt ctgaagcgat gattgatatt agatataata
     3661 tttttttatg ctggaaaaaa ggaataattg atgaagaaac aaagctattt atgatcaatt
     3721 tagctaagag aatttacttt ccttttcgga gttatgagaa tattatcaaa aaatctctcc
     3781 atttttttcc ttcaaaagaa aaatatattt caagattcag taattatatt tttatcaata
     3841 aagttagtct aaaagagtta gatgccatac gagcacttga atatatatca tcattaccga
     3901 aaacataatt attaaattta aattttattt ctcaggctat tgcttttcaa aaactatttt
     3961 atatcttacc tcgtaatcta tagtatgact gagaatctgt ctaaatcaaa cgctcaatat
     4021 aaaacaaact gtaaaaaaat attagaacta tcacctaaaa ttcgttatgt aggtgtaaca
     4081 aataaatttg gtagaacaat agcgggccaa cttagaattg gtttaactcc ctttttaaaa
     4141 acagaagaaa tgaggaacga attgtttgtt cattctatga tttataatat acgaaaaaac
     4201 tatgaaaaat ccataggaga aaccgaattc atttttattt ctaataaaaa tgtggatatt
     4261 attagttttt atattgatga attagtttat tatattacta tagacaagaa cgtagacaaa
     4321 aatgaattgt acacccttat cgaacaaact aaagatcttg caatatcatg atttatttca
     4381 aatctatttg gtttaaatta agttaaagat tttaattttc cccgcatctc tttatcttta
     4441 taaattgatg gatgacttgg atcagctaat atcacctcat accatgagtg tttaccatct
     4501 ttatacagaa aataggaacc aattatttcc atatttggat atttctcatt aactcttctt
     4561 tcagctacca accgcatatt cgttgcttgt ttaatatgaa caactccact gtgtttaggt
     4621 cttctgccgg cgactggtcg ctgctttcgc attcctcctc ttcctactcg cgctctaatc
     4681 actacaatac cctgcttggc tttatatccc agtcttcttg ctctatctaa tctactaggt
     4741 ttatttattc tatgaactga tagctctttt ctccatccaa ctgctctcga ttttaattcc
     4801 tcgctatttg ttttccacat tttagtccac gttgacgcta actgtctgta cattttaaaa
     4861 tataatcaaa ttctttcaaa agactatata agaatttctt ttattgcacg gctatcattc
     4921 ttttagtata taaaaaatat tattacgata agtaatttta aattgaaact ataaaatcta
     4981 tttctatcgt gcttcctaat ggtagactat cgaccccaat agcgatccgc gaatgttttc
     5041 ctttttctcc aaatagagtt tccaaaagat ttgatgcaga attgattact gttggatgat
     5101 ctgaaaaaga ctcaacgcag tttatatatg cagatacctt tactattttt ttgatcttat
     5161 caaggcttcc taaatgatct ttcaataatg atagagcgtt aagagtacaa agtattgctg
     5221 cattttgacc ttcttcaagt gttgtatctt ttcctatttt ccctttgtac tttattgtta
     5281 ctggatttga accggtatct aaaggaattt gtccagaaac aaagattaga ccatttactt
     5341 caactaatgg aatataagaa ccagaagatt ttggaggtga gggcaattcg aatcctaagg
     5401 aatatagttt ttcatcaatg cttatcaatt ataaaatatg aaaaagttgt attattaaat
     5461 gtattaaaca attaacataa attaataaaa ataatatttg aatgcatgat aaagcgagtt
     5521 ttattgtcat tagctatagc atctgtgatt acttatttcg tttatctagg tctgatggat
     5581 tctatgacta gatcttcacc taattatctc tctatttcta atgatactgc tgtttccatt
     5641 atttttaaaa atgaaaacat atcctctgac catctcaaaa ccgtaaattc cgagtatgta
     5701 tatataaaag gaaatgggga aatatatgaa tcaaaaaaag atactaactt tataggcaat
     5761 tacgtaagaa aaaattcccc tactattact actggaaatc attttggatg ggaaatcaac
     5821 ttaccggcat taaatcaaac ttattatgtt gatcatttaa cgggagaaat tatctccact
     5881 aattaaaatc cttttaattt attactaagg ttctaaataa agcaagtaat aattatattt
     5941 gtatctcaaa aaattttata catgcaactg actggaaatg tattaatctg tgacccaata
     6001 tccaaaatag gaataacaat tttagaaaaa gcaggcctta atgttgtcga taaaccatcg
     6061 ataactaatt ccgaattatt atccgaagtt gaaaactatg atgtaattgt agtcagaagt
     6121 aggaccaaaa tcactagtga agttatttca aatgcaaaaa aagctaaaat tgtagctcga
     6181 gttggtgtag gtcttgacaa catagatgtt gaatctgctc tacaaaataa tattgaagta
     6241 gtaaactcac ccgaatctgc aataaatgcc gttgctgaat tagttattgg attaatgctt
     6301 tcattagcga gaaatttacc tttagcagat tgtgaaatta agaaagggaa ctggctgaaa
     6361 aaggaactga atggaattga attgagagga aaatatttag gtattgtagg agttggaaat
     6421 attggaagga acttggcaag aattgctagg tcattacgga tgaatattat aggttatgat
     6481 accattccta ttcaacaaga ctttataaaa gaagtaggta tgataacaac ggattttaat
     6541 accttaatag caagttcaga ttttatttca tgtcatgtcc catcaacgcc tcatactagg
     6601 cacatgttca atgaatctag tttctcaact atgaaaccca ctgctttttt tataaatgca
     6661 tcaagaggag aagtagtaga tgaaaacgct ttgtacaacg ctctaacaac aaaaaaaatt
     6721 gctggagctg cattggatgt ttttgaagta gaaccaccta ctaataaaaa attgttgcaa
     6781 ttatcaaata tcatctgtac tccccatata ggctctcaaa ctaaagaatc tcaagaatta
//
LOCUS       DATAFW010000158         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5225234, whole genome shotgun sequence.
ACCESSION   DATAFW010000158 DATAFW010000000
VERSION     DATAFW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5225234"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1087)
                     /locus_tag="VHJ57_03670"
     CDS             complement(233..1087)
                     /locus_tag="VHJ57_03670"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX2487662.1"
                     /translation="MNFPVLNSDNSFSNEKELEFVDLSSSSSLSANTNESKAFAKDHF
                     YNAFPQITDIQSYIAETEKRVLEAFEKGSRLSPRIETSDQFLQQHAGSKLKLVVLYVD
                     LVDSTLMTRDFSVDKLATIIQMFTQEMSVAASNFNGQVLKYVGDAVIAYFPIEVNDSI
                     SCSSAINCAFHMITIIQQGINPVLRRNNYDQIEVKIGIDASEHSIIQYVLGEKSYIDI
                     LGYGISMSAKLTKLANPNQIITSHRIYMNMHPSLRKRFSELEIAPRIWKYTDEKIQPG
                     VWKSLDKI"
     gene            1312..2160
                     /locus_tag="VHJ57_03675"
     CDS             1312..2160
                     /locus_tag="VHJ57_03675"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX2487663.1"
                     /translation="MEYVHHSVLVSTQWINDHLDDLKIRIVEIDYIPETSYILGHIPG
                     AVLFDWKLDIIDSKKRDVISKEDLEASLQRIGVNNDTTIIVYGDFKNWFATFAFWIFK
                     YYGVQDVRLMNGSRKKWLEEDRPISVDTPSYPRGNFKASGPDESIRVFMNYVKDVIGS
                     QDKILVDVRTNDEYNGKTLAPTEYSTEYGQVGGHIPGAVNVPWSKMVNEDGTLKSADE
                     LKNLYESVNVTPDKEIITYCGIGERASYTWFVLKYLLGYPNVKTYDGSWLEWGNTMGN
                     PIENEK"
BASE COUNT          810 a          292 c          359 g          722 t
ORIGIN      
        1 agaataataa gaaaaatatt attgttcact gtattaatct aaataatata atatataata
       61 attgaaggag aaattgtcaa gataactgaa taaattaaat aaacatctat aaatgattaa
      121 ataacaaaga caataaataa aaaatatcac tatcaatata gcatacaatt atcaaataga
      181 aaaattttac aactctaaaa caaatattaa cagattttaa aaaatagtct cttcatattt
      241 tgtccaaaga tttccaaaca ccaggttgaa ttttttcatc agtatatttc catattctag
      301 gagctatctc caattctgaa aatctttttc ttagtgatgg atgcatattc atgtagattc
      361 tatgactagt aataatttga ttaggatttg ctaatttagt caattttgct gacatgctta
      421 tgccatatcc caaaatatcg atataggatt tttcacctaa tacgtattga ataattgaat
      481 gctcacttgc atctattcct atctttactt caatttgatc ataattattt cttctcaaga
      541 caggattgat tccctgctga ataatagtaa tcatatgaaa tgcacaattt attgcagaac
      601 tacaagaaat agaatcattt acttcgatag gaaaatatgc tattacagca tctccaacat
      661 attttagtac ctgtccgtta aaatttgaag cagcaacaga catttcctgt gtaaacatct
      721 gtattattgt ggctagcttg tcaactgaaa aatctcttgt catcaatgta gaatcgacca
      781 gatctacata tagtacaaca agtttcagtt tagaacctgc gtgttgttgc aaaaactgat
      841 cagaggtttc gattcttgga gaaagtcttg aacctttttc aaaagcctct aaaactcttt
      901 tttcagtctc tgcaatgtat gattgaatat ctgttatttg aggaaaagca ttatagaagt
      961 gatcttttgc aaaagcctta ctttcatttg tatttgcaga taatgatgat gaggacgata
     1021 aatctacaaa ctccaattcc ttttcattag aaaaagaatt atcagaattt aagacaggaa
     1081 aattcaagtt tctatgcgta caagaaatct atacttacat aaaaagtcaa cttattgtta
     1141 aaaattaata aagaaacaaa caatttatag acatacgtga caaaaataaa gagatttctt
     1201 attaatgaac taatatgatt gctatttata gtagtaatca acgtattttc tagaaacaat
     1261 taaagagtaa agattgtaga atatatacgg aaattaaatt agtgtattat aatggagtat
     1321 gttcatcata gtgttttagt tagtactcaa tggataaatg atcatttaga tgatctcaaa
     1381 attcgaatag ttgagattga ttatattcct gagacaagct atattctagg acacattcca
     1441 ggagctgtat tgtttgactg gaagctagat ataatcgatt caaaaaagag agacgtaatc
     1501 tcaaaagagg atttggaggc atcattacag cgaataggtg taaacaatga tactacaata
     1561 atagtttatg gtgattttaa gaattggttt gcgacatttg ctttctggat tttcaaatac
     1621 tatggagttc aagatgttag gttaatgaat ggttcaagaa agaaatggct ggaagaagat
     1681 cgtccaatca gtgtagatac tccatcttat ccaagaggga acttcaaagc ctctggtcca
     1741 gatgaaagta taagagtttt tatgaattat gtaaaggatg taataggttc tcaagataag
     1801 atactagttg atgttagaac taatgatgaa tataatggaa aaacgcttgc tccaacagaa
     1861 tattctactg aatatggtca ggttggagga cacattccag gagcagtaaa tgtgccatgg
     1921 agtaagatgg ttaatgaaga tggaactctc aagtcagcag atgaattgaa gaatctttac
     1981 gaatcagtaa atgttacccc tgataaagaa ataataactt attgtggtat tggtgaaaga
     2041 gcatcatata cttggtttgt tctgaaatat cttcttggtt atcccaatgt aaagacttat
     2101 gatggttcat ggttggaatg gggtaatact atgggaaatc ctattgaaaa tgaaaaatga
     2161 atgaaagaat taaattattt ttt
//
LOCUS       DATAFW010000159         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2053117, whole genome shotgun sequence.
ACCESSION   DATAFW010000159 DATAFW010000000
VERSION     DATAFW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053117"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..875
                     /locus_tag="VHJ57_03680"
     CDS             84..875
                     /locus_tag="VHJ57_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487664.1"
                     /translation="MLSVQEWKRWFLYQLIGEGEHNAIYHEHIIRELGKHYDSNLERA
                     LRDLIDDGIIMLIESRKIKKYIVNFEFLSEAEMILNLDKNIQVQQTNKIHDHNNVNYM
                     TSTNEKRFVQPYLPEPSGYVYWFDNEENRKFKKQSVYRIYFKETDKTNFAAQLITKSI
                     GSSKIIYMGSINEPNSYISRLWRAVLSVSQENKEGTFILQDIQDKDRMACGNNRQRGK
                     IALSIFKKLGYIQQSEFKGNSIRFKVSGRKPYSITLDEIFNLKTK"
     gene            complement(1093..1575)
                     /locus_tag="VHJ57_03685"
     CDS             complement(1093..1575)
                     /locus_tag="VHJ57_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487665.1"
                     /translation="MSSDHETDSSVENIKSWEVESLSSVEEFDDYSKALDLFNIKSDQ
                     DEEPILYEIQKSKISGSIIKRIPILNIKQSKKRKEELEKKKLPPQSPNQEQQKKTTSQ
                     TKNKPQGIKYRIVILVIIIVAFLILLYVLSQLVTSSDTLNAHFSLGQLKKNSDISLIA
                     "
     gene            complement(1663..2550)
                     /locus_tag="VHJ57_03690"
     CDS             complement(1663..2550)
                     /locus_tag="VHJ57_03690"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX2487666.1"
                     /translation="MIREVTIVNKVKNYYELTKPKIWYLLVFTAFGSALTASLLFDIP
                     ISPLTWILLLGGVAAGSAAADTITGYNDRDIDAIMDRTKDRPIPSGRISPRNALIFGL
                     ILTVIALIFSWLINLWAFLLMIFGLFDNIIIYSKWLKRRSQSNIVLGGFSGGVPALIG
                     YVTVTTQNIEIGLVMAGLVFLWIPTHIWSLALHVRKDYQKAKVPMLPVVSEEKRAVRI
                     IAVTTLIMVLFSILPFFFNQFGHIYLVTATIFGAIMMGLSLWLLIKPTEKASWTVFKF
                     SSPYLTAVFIAFMVDAFFH"
     gene            complement(2639..>3067)
                     /locus_tag="VHJ57_03695"
     CDS             complement(2639..>3067)
                     /locus_tag="VHJ57_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEX2487667.1"
                     /translation="TKKILGTLKNGSISSSTFKVSCTCTRVPVMDGHTETVFVETQKP
                     LEPDQVKECMIKFSKSIKIKGLPSSPTEYIIVHDDPTRPQPRIDREINDGMTTFVGRL
                     RKDTVFDNGIKYVLLSHNEKMGSAKGAVLLAELLKTKNMI"
BASE COUNT         1078 a          453 c          482 g         1054 t
ORIGIN      
        1 tacaaatgtt tattacaaaa tcttaatgat taaaatttga tctttatgct tatatattcg
       61 tagaaaatag cttttaatat cgaatgctta gtgtacagga atggaaaaga tggttcttat
      121 accaactaat tggtgaagga gagcataatg ctatatacca cgagcatatc ataagagaac
      181 ttggaaagca ttacgatagt aacttggaaa gggctttgag agatttaatt gatgatggaa
      241 taataatgtt aattgagtca aggaagatta aaaagtatat tgtaaatttt gaattccttt
      301 cagaggcaga aatgatattg aatttagata aaaatattca agttcaacaa actaataaaa
      361 ttcatgacca taacaatgtg aattatatga cttccacaaa tgagaaaagg tttgttcaac
      421 cttatcttcc agaacctagt ggatatgttt actggtttga taatgaagaa aataggaaat
      481 ttaaaaaaca aagtgtctat agaatatact ttaaagaaac ggataagacc aattttgctg
      541 cacaattaat aacaaaatca ataggcagtt caaaaataat atatatgggt tctataaatg
      601 aacctaattc ttatatatct cgactatggc gagccgtatt atctgtttcc caagagaaca
      661 aagagggaac atttattctt caagacatac aagacaaaga tagaatggca tgtggaaata
      721 atagacaaag aggaaaaatt gcattatcta ttttcaagaa attaggctac attcaacagt
      781 cggaattcaa aggtaattcc attcgattca aagtaagtgg tagaaaacca tattcaataa
      841 ccctagacga gatttttaat ttaaaaacta aataattaga agtatgtgta ggagatgatt
      901 tagttaaatc attgagatat ctattattgt cttagactat aataattaat cttggtttaa
      961 agaatgtagc gattaataga ttccattcac tctagaattt tagaaaggtt tagtttagtc
     1021 gatccagtgt ctttatgcta ggcattttgt gtgttttact attactaatt gaggatcttt
     1081 tttcaaaaag aatcaagcaa ttaaagaaat atcactattc ttctttaatt gaccaagtga
     1141 aaaatgagca tttaatgtat cagatgaggt gactaattga cttaaaacat ataataagat
     1201 taaaaatgca actattataa ttaccaagat tacaattcga tattttatac cttgaggttt
     1261 atttttagtt tgtgaggtag tttttttttg ttgttcttga ttaggggatt gtggtggtag
     1321 tttttttttt tctaattctt cttttctttt cttagattgt ttaatattta gaattgggat
     1381 tctcttaata atagaaccac ttatttttga cttttgaatc tcatacaaaa ttggctcttc
     1441 atcttgatct gatttgatat taaacagatc aagtgctttt gaatagtcat cgaattcttc
     1501 aactgatgat aaggattcta cttcccatga ttttatattt tctactgaag agtccgtttc
     1561 atgatctgat gacattaatt atagtaatat tgggatatta tttaataatt tacttgtgtt
     1621 aataataacc aatgttaata gagttaattt gtccatatca tattaatgaa aaaatgcatc
     1681 taccataaat gctatgaaaa ctgctgtaag ataaggactc gaaaatttaa aaactgtcca
     1741 tgatgctttt tctgtaggtt taattaaaag ccataaagaa agtcccatca ttatagcacc
     1801 aaatatagtt gcagtaacta aatatatatg tccaaattga ttaaaaaaga aaggcaatat
     1861 actaaataaa accattatca aagtagttac cgcaattatt cttactgctc ttttctcttc
     1921 acttacgaca ggtaacatgg gaactttggc tttttgataa tcttttctta catgtaaagc
     1981 taagctccag atatgtgttg gtatccataa gaagacaaga ccagccataa ctaaaccgat
     2041 ttctatgttt tgcgtagtta cagtaacata ccctattaag gcgggcactc cacctgaaaa
     2101 tccccccaga acaatatttg actggctacg tctttttagc catttgctgt aaataataat
     2161 attatcaaat aaaccaaaaa tcattaataa aaaagcccac aaattaataa gccacgaaaa
     2221 tatcaatgca ataacagtca gaataagacc aaagattaat gcattcctcg gtgaaattct
     2281 gcctgatgga ataggcctat ctttagttct atccattata gcatcgatat cacggtcatt
     2341 ataacctgta atagtatcag cagcagctga acctgctgca actcctccca gaaggaggat
     2401 ccaagttaaa gggcttattg gtatatcaaa taataaagaa gcagttagag cagatccaaa
     2461 ggcagtaaaa actagcaaat accatatctt tggcttagtt aattcataat aattttttac
     2521 cttgttcacg atggtgactt cgcggatcac tctctagatc tcaactcatt ttcaactaac
     2581 ctaatttatc aataggttta atattaattc ttcaaattga attacatgtt gataaaagct
     2641 aaatcatatt tttagttttg agcaattcag ctagaagtac tgctcccttg gcagagccca
     2701 ttttttcgtt gtgagaaagc aagacatact ttataccatt atcaaatact gtgtcttttc
     2761 tcaatctacc tacaaatgta gtcattccat cattgatttc tctatctatt ctaggttgag
     2821 gtctagttgg atcatcatgt actattatat attcagtcgg agaagaaggt agacccttta
     2881 tttttattga tttagaaaac tttatcatgc attctttaac ttgatcaggt tctaaaggct
     2941 tttgtgtttc aacgaaaaca gtttcagtat gtccatccat tacaggaact cttgtacagg
     3001 tgcaacttac tttgaaggtt gatgaagaga ttgaaccgtt tttgagtgta cctaagattt
     3061 tttttgt
//
LOCUS       DATAFW010000160         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1172192, whole genome shotgun sequence.
ACCESSION   DATAFW010000160 DATAFW010000000
VERSION     DATAFW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172192"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..2105)
                     /locus_tag="VHJ57_03700"
     CDS             complement(33..2105)
                     /locus_tag="VHJ57_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HEX2487668.1"
                     /translation="MSELQKTESALVNDLEEFLRAFKDRTGNYKYFDRINNLMAANST
                     SLVVDYIDFDTFKPDLAKLITNEPDEMFDAFNRAIFNILYEIHPDYANETQNNIKVRI
                     GNYTVQKSLREINAEVINKLVGVSGMVVRSSEVKPLAKKIGYRCLNCGEITEAQLRGL
                     VLKKPLRCGKCSEKELEMDPDTSIFIDFQMVRLQELPEDLPAGHLPHYLEVTIIGDLV
                     DQCRPGDRILLTGIVRIEQEQLSQQIKTSLFRLRMEGNNIEYLGGRTADKDSRTIERL
                     EISNEDEKHIFALARKKDAYDILISSYAPHVYGHEVIKEVILLLIVGSVTKRLEDGSA
                     RRGDINVLLVGDPGTAKSEMLKFAAKIAPRGLYTSGRGSTAAGLTAAVIRDKSGIMML
                     EAGAVVLGDQGLVCIDEFDKIKPEDRSALHEVMEQQTCSVAKGGIVATLNARTSILSA
                     ANPLYGKYDPFKNITENVNLPIPLLTRFDIIYVIRDIPEKEKDHRIASHILELHRESE
                     RTGQYLIEIDLFSKYLAYSKTMEPKLTVEAIDKIRDYYMKMRNVESEGMITVTPRQLE
                     GLVRLATARSKLLLKDYVDSEDAERAIYLIQTMLETAGVDVNTGRVDLGVLHGKPQSE
                     ISKLKLFMEVFNGLSGQDKNDVEEKNFINELIQTGRFSEDDAKNFLNKAMQNGQIYER
                     RSGFYAKA"
     gene            complement(2102..2701)
                     /locus_tag="VHJ57_03705"
     CDS             complement(2102..2701)
                     /locus_tag="VHJ57_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487669.1"
                     /translation="MNEITSYNRLDQVMIAHNNDNVDRYDHFKKQIDFIQKFYSIKYL
                     MEETRIAFKKNFSINIDGLDLEAKEGEIGSIPRWLAQILEENNSIEIQDTDVLIYISR
                     SLNRERISKPHDLSGIDLDFYIRANDFIKRLKDKEKESIVVSLNSFVTSRIEKIVKLA
                     AASALSVDLEKKLSAEEKELYNFIHKSSSEFKQRAVNRS"
     gene            2830..>3642
                     /locus_tag="VHJ57_03710"
     CDS             2830..>3642
                     /locus_tag="VHJ57_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HEX2487670.1"
                     /translation="MSSNTINTKLSSLMWVEKYRPKRIEQIVNQKDIITSLENLIKKP
                     DEIPHLLFTGPAGVGKTTTALCVAMELLGEGWKRDTLELNASDERGIKMVRERVKEFA
                     AVIKLPSGTNEKGEYFSKDEKPYRIIILDEADEMTSEAQTALRRIIEDSSKTTRFIII
                     CNYLSQIIEPIQSRCAIFRFTRLEEKDVLEYLRMICNQEKVDFQEEALSKIYRYTEGD
                     LRHSINILQTASGYGQVSIKNVTSAIGLSGKAKVGEIIRLALLGKFNDARIRL"
BASE COUNT         1204 a          615 c          517 g         1306 t
ORIGIN      
        1 aaatatgcaa taatataatt aatagtattt atttatgcct ttgcatagaa gcctgaacgt
       61 cgttcatata tttgtccatt ttgcattgct ttatttaaaa agtttttggc atcatcttct
      121 gaaaaccttc ctgtttgaat aagttcattt ataaaatttt tttcttctac atcattttta
      181 tcttgtccag ataaaccatt aaaaacttcc atgaatagct ttaattttga tatttcactt
      241 tgaggtttac catgtaaaac tccaagatct actcttccag tattaacatc tactccagcc
      301 gtttctagca tagtttgaat tagatatatt gcacgttcgg catcttccga atctacataa
      361 tcttttaata ataattttga acgagcagtt gctaatctga ctaatccttc aagttgccta
      421 ggtgtaacag taatcatacc ctctgattct acatttctca ttttcatata gtaatctctt
      481 atcttatcta tagcctcaac agttaattta ggttccatag tttttgaata agcaagatat
      541 ttactaaata gatcaatctc aataagatat tgtccagtcc tttccgattc tctatgtaat
      601 tctaatatgt gactagcaat acgatgatct ttttcctttt ctggtatatc tctaattaca
      661 taaataatat caaatcttgt taataatggt attggtaagt taacattttc tgtgatattt
      721 ttaaagggat catatttacc atataatgga tttgcagctg acaatataga agttcttgca
      781 tttaatgtag caactattcc tccttttgcc acagaacagg tttgctgctc cataacctca
      841 tgtaaagctg aacgatcttc tggttttatt ttgtcaaact catctataca aactaaacct
      901 tggtctccta gtactactgc ccctgcttcc aacatcataa taccggattt atctcttatt
      961 acagcggcag tcaaacctgc agcagtcgaa cctcttcctg aagtatataa tcctcttgga
     1021 gcaattttgg cagcaaattt taacatttct gattttgctg tgcctgggtc acctactaaa
     1081 agaacattaa tatctcctct tctagcagat ccatcttcta atctttttgt tactgatcct
     1141 actattaata ataaaattac ttctttaatt acttcatgtc catatacatg aggagcatat
     1201 gacgaaatta aaatatcgta cgcatctttt ttacgtgcta atgcaaaaat atgtttctca
     1261 tcttcgttac tgatttccaa acgttctatt gtcctactat ctttatctgc agtccttcca
     1321 ccaagatatt cgatattatt tccttccatt cgtaatctaa aaagacttgt tttgatttgt
     1381 tgtgataatt gttcttgttc tattcttaca attccagtta ataatattct gtctcctggt
     1441 ctacattgat ctacaagatc tcctataata gttacttcta aataatgtgg caagtgacca
     1501 gcaggaagat cttctggaag ttcttgtaac cttaccattt gaaaatcaat gaaaatactg
     1561 gtatccggat ccatttctag ctctttttca gaacatttac cacatcttaa tggttttttc
     1621 aatactaatc ctcgcaattg agcttctgtt atttcaccac aattaaggca tctataacca
     1681 attttttttg ctaatggttt tacttcagat gatctaacaa ccattcccga aactccaaca
     1741 agtttattga tcacttctgc atttatttca cgtaaacttt tttgtactgt ataattgcct
     1801 attctgacct ttatgttatt ttgagtctca ttcgcataat ctggatgtat ttcatagaga
     1861 atattaaaaa ttgctctatt gaatgcatca aacatttcat caggttcgtt agtaattagt
     1921 tttgccaagt ctggcttaaa agtatcaaaa tcgatgtagt caacaacaag cgaggtagaa
     1981 ttggccgcca tcaagttatt aatcctatca aaatatttgt aatttccagt tcgatctttg
     2041 aaagcacgca agaattcctc taaatcatta accaatgcag attcagtttt ttgaagttca
     2101 ctcatgacct atttacagcc ctttgtttaa attctgaaga agacttatgg ataaaattgt
     2161 aaagttcttt ctcttcagct gacagtttct tttccaagtc aacagataag gcagacgcag
     2221 ctgctaattt aacaattttt tctattcgag aagttacaaa tgaatttaga gaaaccacaa
     2281 tagattcttt ttctttatct tttaatcttt tgataaaatc gttagcacga atgtaaaaat
     2341 ctaggtcaat gcctgaaaga tcatgaggtt ttgatattct ttctcgattt aaagatcttg
     2401 aaatataaat tagaacatcc gtgtcttgaa tttctatgga attattttcc tctaaaattt
     2461 gtgctaacca tctaggaatt gaacctattt ctccttcttt tgcttcaaga tcaagtccat
     2521 caatattaat agagaaattt tttttaaagg cgattcgtgt ttcttccatt aagtatttaa
     2581 tagaataaaa tttttgaata aaatctattt gttttttaaa atgatcgtaa cgatcaacat
     2641 tatcattatt atgagctatc atgacttggt ccaatctatt atatgatgtt atttcattca
     2701 agactaataa ttcttattaa caaaataaat ttgagtaatt agtataataa attatcttac
     2761 tgccatatta ctatagaata agggaaaaga agatattaac taactacaga aattaggtac
     2821 ttaaatcata tgtcctcaaa cactataaat acaaaacttt caagtttaat gtgggtagag
     2881 aaatatcgac ctaaaagaat tgaacagata gttaaccaga aagatattat aacttcactt
     2941 gaaaatttaa taaaaaaacc tgatgaaatt ccacatctat tatttactgg gccagcaggt
     3001 gtgggtaaga ctactacagc attatgtgta gccatggaat tattaggaga gggatggaaa
     3061 agagatactc ttgaactcaa tgcttctgat gaaagaggaa taaagatggt acgtgaaaga
     3121 gtaaaagaat ttgcagctgt aattaaactt ccaagtggaa ccaatgaaaa aggagaatat
     3181 tttagtaaag atgagaaacc ttatagaata ataatacttg atgaagcaga tgaaatgaca
     3241 tcagaggcac aaactgcttt aagaagaata attgaagata gttctaagac tactcgcttt
     3301 attattatat gtaattatct ttctcagatt atagagccca tccaaagtag atgtgcaata
     3361 tttcgcttca caagacttga agagaaagat gtattagaat atttaagaat gatttgtaat
     3421 caagaaaagg tagattttca ggaagaagca ttatcaaaaa tttacagata taccgaaggg
     3481 gatttgagac attcaataaa tattcttcaa acagcttcag gatatggaca agtttcaatt
     3541 aaaaatgtaa catctgcaat tggactttct ggtaaagcaa aagtaggaga aataatacga
     3601 ttagcactat taggtaaatt taatgatgca cgtataagac tt
//
LOCUS       DATAFW010000161         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_115941, whole genome shotgun sequence.
ACCESSION   DATAFW010000161 DATAFW010000000
VERSION     DATAFW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115941"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1967)
                     /locus_tag="VHJ57_03715"
     CDS             complement(<1..>1967)
                     /locus_tag="VHJ57_03715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase I"
                     /protein_id="HEX2487671.1"
                     /translation="KNDNIYQYKYEIDPKIRNKLSELIDNNNLALLDTKTINYNYYIS
                     QWVNLLNQPIPDLKRIAIDIEVESEEGRIPHPRDHDKKVTAIGLAGSDGFRKVYVLNK
                     DSNEKNAFISEAEVVETEKELILAAFKDIKNYPIIITFNGDDFDLPYLYMRAQDPRID
                     PLNKEIIKKEDIPIVVKRDTFIKRGMQAEPVNLKHGIHIDLFRTFQNRSIQIYAFNHK
                     YAEYTLNAISESLLNDHKIEFEGDISDLPIHKLAEYCLKDADLTLGLSTFNDNLLMKL
                     LIVISRISHLPIDDVSRFGVNQWIRGMLYFEHRQNNFLIPRRDELISKGVSSTTAIIK
                     EKKYRGGLVVEPKLGIHFNVVVVDFASLYPSIIKVHNLSYETVNCSHLSCKNDPEQQI
                     PETTHWICKRRRGLTSLLIGSLRDLRVRYYKHLSKDKTTTTDYRQLYEVISQAIKVIL
                     NASYGVMGAEIFPLYCLPVADATAAIGRVTTTRTIEKCKQIGIEVIYGDTDSLFLKNP
                     SSAKVKEISEWARTQLEVDLEIDKTYRYVVFSDLKKNYLGVLEDGTVDVKGLTGKKSH
                     TPPFIRKAFYKILEILKEVNSEKDFQKAKENIKKIIRIYANDLETGKIHPSELSFNVM
                     VNKSPDRYGEKTNNSKVKSIDGKNIDFITYKG"
BASE COUNT          578 a          327 c          279 g          783 t
ORIGIN      
        1 cctttgtatg ttatgaaatc aatatttttt ccatctattg attttacctt agaattattt
       61 gtcttttctc catatcgatc aggtgatttg ttaaccataa cgttaaaact caattctgaa
      121 ggatgaattt taccagtctc taaatcatta gcatagatcc ttattatctt tttgatattc
      181 tcttttgctt tttgaaaatc cttttcagaa tttacttctt ttagtatttc caggattttg
      241 tagaaagctt ttcttatgaa tggcggtgta tgtgattttt taccagttaa tccttttaca
      301 tctactgttc catcttcaag tactccaagg taattctttt ttaaatcact aaaaacaaca
      361 tatctgtacg ttttgtctat ttctaagtct acctctaact gagtccttgc ccattctgaa
      421 atctccttta ctttcgcaga agatgggttt tttaaaaata aagaatctgt atcaccatat
      481 attacttcaa taccaatttg tttacatttt tctattgttc gtgttgttgt tacccttcct
      541 atggctgcag tagcatcggc tactggtaga caatataatg gaaagatttc tgctcccata
      601 acaccataac tggcatttag tattaccttt atagcctgac taattacttc ataaagctgt
      661 cgataatctg tagttgttgt tttatctttt gaaaggtgtt tatagtatct tactctcaag
      721 tctcgtagag aaccaatgag aagagatgtc aaacctctcc ttcttttaca tatccaatga
      781 gtagtttcag gaatttgttg ttctggatcg tttttgcaag ataggtgaga acaatttaca
      841 gtttcatagg aaagattatg aacttttata atacttggat aaagtgatgc aaaatcaaca
      901 actactacat taaaatgtat tcctagcttg ggttctacta caagaccacc gcgatatttt
      961 ttttctttga ttatagcagt agtggatgat actcccttag atatgagctc atctctccta
     1021 ggtattaaaa aattattttg tctatgctca aaatataaca ttcccctgat ccattgattt
     1081 actccaaatc ttgaaacatc atcaattggt aaatgcgaaa ttcgagaaat tactattaat
     1141 agtttcatta aaaggttatc gttaaacgta gaaagtccta atgtgagatc agcgtctttt
     1201 aaacaatact ctgccaattt gtgtatagga aggtctgata tgtctccttc aaattctatt
     1261 ttatgatcat taagtaatga ttcggaaata gcatttaacg tatattctgc gtatttatgg
     1321 ttaaaagcat aaatctgtat agatctattt tggaacgttc taaatagatc aatatgaatt
     1381 ccatgtttaa gatttactgg ttctgcctgc attcctcttt ttataaaagt atcccttttt
     1441 acaacaattg gaatatcttc tttttttata atttctttat ttaatggatc tattctgggg
     1501 tcttgcgccc tcatgtataa atatggaaga tcaaagtcat ctccattgaa cgtaataata
     1561 atggggtaat ttttaatatc tttgaatgcc gctaggatta actccttttc agtttcaact
     1621 acttctgctt cagaaataaa ggcatttttt tcattcgaat ccttatttaa gacgtatact
     1681 tttctgaaac catcactccc tgctaatcct atagcagtaa cttttttatc atggtctcgt
     1741 ggatgtggaa ttcgtccttc ttcagattct acttcaatat ctattgcaat tctttttaga
     1801 tccggaattg gttgatttaa tagattaacc cactgagata tataataatt gtaattaatt
     1861 gttttagtgt ctaatagagc taaattattg ttatcaatta attcagaaag tttattacgt
     1921 attttaggat ctatttcata tttatattga taaatattat cattttt
//
LOCUS       DATAFW010000162         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2758910, whole genome shotgun sequence.
ACCESSION   DATAFW010000162 DATAFW010000000
VERSION     DATAFW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2758910"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..714)
                     /locus_tag="VHJ57_03720"
     CDS             complement(55..714)
                     /locus_tag="VHJ57_03720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX2487672.1"
                     /translation="MKIKSKPNDLNNIVLKVLAEIEETARKEFLPSIGPIKGKIIEDV
                     IREHKPRKVLEIGTLHGYSAILIANIILGEKYGKENFASKDGGSDPILISMEKDQKLA
                     KIARMNIENSRLSKKIQVIDGDALEVIPRLKSKFNMIFLDATKSEYLKYLQLIEKYSL
                     LNKRAVVVADNVLIYENEMKDYLDYVRNSGKYISRTTETTLEFTKNVKDALEVSINVD
                     I"
     gene            865..1281
                     /locus_tag="VHJ57_03725"
     CDS             865..1281
                     /locus_tag="VHJ57_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX2487673.1"
                     /translation="MSEQSNGMLSDPRIRELFESKNFAFLSSLMKDGSPHVTPTWVDI
                     ENGNILVNTAVGRIKHRNISRDPRVALSIIDQNNQYEMVTVRGKVTEQITGETAEKHI
                     DKLSKKYIGVDKYPRRSKREQRVILKIKPEKIFHMK"
     gene            complement(2080..>2539)
                     /locus_tag="VHJ57_03730"
     CDS             complement(2080..>2539)
                     /locus_tag="VHJ57_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX2487674.1"
                     /translation="LSLSIRKILVTIDGSENSKKAADYAISFSKKYNSQLIVLYVLYS
                     ELGFAYSNLLGVTTPKAIRDILETQKKDVQKWFDEIKSKLKNTNISVADKIIISVSSI
                     VGEIVGFADKEKVDLIILGTRGRTGFKKLLLGSVAQGVVSHSSCPVMVVR"
BASE COUNT          816 a          437 c          362 g          924 t
ORIGIN      
        1 atgatcactg tcgtgtaaac gtttgacgat aaaacaccgg agctcactcc agtactatat
       61 atcaacatta atacttactt caagagcatc tttcacattt ttagtaaact ccaatgtagt
      121 ttctgttgta cggctaatat acttgcctga attcctaaca taatccagat aatccttcat
      181 ttcattctca tatattagca cattatcagc aactactact gctcttttat ttagtaaact
      241 atacttttca ataagttgaa gatattttag gtattcactc tttgttgcat ccaagaatat
      301 catattgaat ttggacttaa gtctcggaat cacttccaaa gcatcaccat caattacttg
      361 tatttttttt gataatctag aattttctat gttcattctt gctatctttg caagtttctg
      421 atccttttcc atactgataa gaatgggatc actaccacca tctttactag caaaattctc
      481 ttttccatat ttctcaccca atataatatt tgcaattaaa attgctgaat atccatgcaa
      541 tgttccaatc tcaagcacct tcctaggttt gtgttcccta ataacatctt ctataatttt
      601 tcccttaatg gggcctattg atggtagaaa ttcctttctt gccgtttctt caatttctgc
      661 caataccttc agtacaatat tgtttaggtc attcggttta cttttaattt tcaagttctg
      721 tactattttc ctacattact ataagaaaat ttctacgcaa ttacatattc ggaattgtcg
      781 tttcacaaaa cttcgatttt tagactattg caagatactt tgagcttaat aaacagttat
      841 tagaaataat gccaatattt gactatgagt gaacaatcaa atggaatgtt aagtgaccct
      901 cgtattagag aactttttga aagtaagaat tttgccttct tatcttcatt aatgaaagat
      961 ggctctccac atgttactcc aacttgggtt gatattgaaa atggtaatat tcttgtcaat
     1021 actgcagtag gtaggataaa acataggaat atttctagag atcccagagt agctttgtcc
     1081 ataattgacc aaaataatca atacgaaatg gttaccgtta gaggtaaggt gacagagcaa
     1141 attaccggag agacagctga aaaacatatc gataaattat ctaaaaaata tattggcgta
     1201 gataaatatc caagacggtc taaaagggaa cagagagtga tattaaagat aaagccagag
     1261 aaaatatttc atatgaagta atgctcttat ttagcttaga aaaaagagac acataatttg
     1321 ccagatttta ttaaatttgt ttgaaccttt acgacaaata gaaaaattga agcccttgtt
     1381 atggcgaatg aattttctta atttgcaaga ttgaaatcgt tatattgtat attgtatatt
     1441 gtacgctatg tcggataatc aagaaaaatg tgcagcatgc ggaatgacat tcaatacgca
     1501 agaggaattg aaagaacaca tgctagaatt acatcgagag taatatttca cctattcaca
     1561 cctagatata attctgcaat cgattagatt gtcctaattt tgattaagct tgtagaccgg
     1621 aggcgatatc ttttcttcca tggaactcag aatctggtct atacagtcat gagtaataag
     1681 tgatggaatt gataccttaa gctctattgg caccaagtca ttaatataac ttaaaagctg
     1741 acaccttttt ctaatgtctt tttcttctcg gcacatattt atgaaactga ttaattgttt
     1801 actatttttt agcatcacca tgttatgggt attcagattt atgaacttta agtattatat
     1861 ttttgattat tgttctatat caataacact agaaattttt agctatattt taacatggta
     1921 attttttagc ttctattatg atgggcaatt aattgttctt tatatgccaa aaaccttcaa
     1981 tctttttaga tttttaaaaa atttggcaga attttctgta tttatatctt ttagattcct
     2041 attatggtca tgttgtttga aagcccgtta taatgtagat tatctaacta ccattacagg
     2101 acaagatgaa tggctaacta caccctgtgc tacacttcca agtaacaatt ttttaaaacc
     2161 agttcttccc ctcgttccca aaattatcaa gtctactttt tctttgtctg cgaacccgac
     2221 aatttcacca acaattgatg ataccgaaat aatgatttta tccgccacag aaatattggt
     2281 gtttttaagt ttacttttta tctcatcaaa ccatttttgc acgtccttct tttgtgtttc
     2341 taatatgtcc ctgatagcct ttggagttgt gacccctaat agatttgaat aagcaaaacc
     2401 taattctgaa tacaacacat ataatactat taattgagag ttgtactttt tagagaaact
     2461 aattgcataa tcagcagcct ttttggaatt ttcagaacca tcaattgtta ctaatatttt
     2521 tcttatactt aatgacaaa
//
LOCUS       DATAFW010000163         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_646995, whole genome shotgun sequence.
ACCESSION   DATAFW010000163 DATAFW010000000
VERSION     DATAFW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646995"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1382
                     /locus_tag="VHJ57_03735"
     CDS             222..1382
                     /locus_tag="VHJ57_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX2487675.1"
                     /translation="MYDSIIFKDRKKLSPRYLPKEIPHREKQIDLLIRTFLDIKDDPD
                     KFPLTVLQIIGPAGIGKTSTVIKFSDILENELRKNKINIKIVYINLKLQGGNKYAIYK
                     YLLSCIAPELPAQGLSAEEMLRQLLDYLSINNMYSLIILDEIDYLIKISKEIGIIYDL
                     TRLNEFDLSKKCNVMGVIFIARSTEFYEKLDEAELSSMGRTYIEFPNYTIDQVSDILI
                     RRSKDAFQENVIGTDIIDWISKIVISPIVNGDIRYALDLLSYAGNLAESEGTERVLLD
                     HVKKINKQIYNGITDEDIIELSKLQIIVLLGIIKGLKIKHKDYVDLKEIRIQSLEIAE
                     KYKLRKLDIEDILDDLATRKIIKIISLKKISFISSSVENLEKTLISKIDSTS"
     gene            1389..1685
                     /locus_tag="VHJ57_03740"
     CDS             1389..1685
                     /locus_tag="VHJ57_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487676.1"
                     /translation="MNDAFQVKDIVEVGLGSIGKIKILRALAEEKKLLTVYRLHKKTH
                     LKREDIKRNVAELVKIEWVIEQKISNSLYSINRENKYVQRLVLFFYEIGYIDNI"
     gene            1870..>2184
                     /locus_tag="VHJ57_03745"
     CDS             1870..>2184
                     /locus_tag="VHJ57_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487677.1"
                     /translation="MKLKNKGTIKIGKMFIFVLIGIFSYCTISTISFAQTQGEDLNVI
                     ITFFDITENTGDVITFINSDGLTKAKIFTPLKSMKMGNTTELQFNLTANQVSMGEKFR
                     VCA"
BASE COUNT          913 a          231 c          341 g          699 t
ORIGIN      
        1 atggatttga aataaatgaa tcaagtttga aaaaagaagc ctttattccg tggatattaa
       61 aaataaatga aagaatttat ttattttact ttgtagactt tattaaagca aaaaaaatct
      121 caaaacataa agtaaattta agtcaatact aaataagaag atttgtgcca aagaataatt
      181 aataatatta agaaaaaata gatggtagtg taacacaata tatgtatgac agcattattt
      241 ttaaagatag aaaaaaatta tcacctcgat atttacctaa agaaatccct catagagaga
      301 agcaaataga tctcttaatt agaacttttt tggatataaa agatgatcca gacaaatttc
      361 ctttaacggt cttacaaata attggtccag caggaatagg gaaaacatca acagttatta
      421 aattctctga tattttggaa aatgaattaa gaaaaaataa aataaatatc aaaatagtat
      481 acataaattt aaaattacaa ggaggaaata aatatgcaat ttacaaatac ttgttaagtt
      541 gtatcgcccc agaattgcct gcacaaggtc ttagtgctga ggagatgctt agacaattgc
      601 ttgattattt aagtataaat aatatgtatt ctttaatcat acttgatgaa atcgattatt
      661 taatcaaaat ttctaaagaa attggaatca tttatgatct tacaagattg aatgaatttg
      721 atctctccaa aaaatgtaac gtaatgggag ttatttttat tgctcgaagt actgagtttt
      781 atgagaagtt agacgaagct gaattaagtt caatgggacg aacatatata gaatttccta
      841 actacacaat tgaccaagta agtgatatcc ttattagacg ttcaaaagat gcctttcaag
      901 aaaatgtgat tggtacagat ataattgatt ggatatcaaa aatagtaatt tctcctattg
      961 tcaatgggga tataagatat gcattagatt tactatcgta tgcaggaaat cttgcagaat
     1021 ctgagggaac agaaagagta ctcttggatc atgtaaaaaa aatcaataaa caaatctata
     1081 acggaattac ggatgaggat attatagaat tatccaagtt acaaataatt gttttgctag
     1141 gaataataaa gggattaaag attaaacata aagattatgt tgatttaaag gaaattagaa
     1201 tacagagttt agagattgct gaaaaataca aattaagaaa attagatata gaagatattt
     1261 tagatgatct tgccactaga aagattatca aaataatctc tttaaaaaaa attagtttta
     1321 tatcatcatc tgtagaaaac ttagagaaga cattaataag taagatagat tcaacttctt
     1381 aatttcaaat gaatgatgcc tttcaggtca aagatattgt agaagtagga ctaggaagta
     1441 taggtaaaat aaaaatatta agagcattag cagaggagaa aaaattatta actgtatata
     1501 ggttacataa gaagacacat cttaaaagag aggatataaa aagaaatgta gctgagctag
     1561 taaaaattga atgggtgata gaacaaaaaa ttagcaatag tctttattca attaatagag
     1621 aaaataaata tgtgcaacga ctggtattat ttttttatga aataggatac atagacaata
     1681 tttaatagtt ctcttctgaa aggtaacata ctaatatatt atatactgga aaagttatct
     1741 ataaaaaaaa gatataattt tatggtttga aaagaaagaa aataattctt aatattatat
     1801 agattgagtt tatataattt aatttgataa acttcatata ttaaaccatg ctatattacc
     1861 tactattcta tgaaactaaa aaacaaggga actataaaaa taggtaaaat gtttatattt
     1921 gtacttattg gtatcttctc ctattgtaca atttcaacga tttcttttgc tcaaactcaa
     1981 ggtgaagatt tgaatgtgat aataactttt tttgatataa ctgagaatac tggtgatgtt
     2041 ataacattta taaattctga tggattgaca aaagctaaga tatttactcc tctcaaaagc
     2101 atgaagatgg gaaatactac agaacttcaa tttaatctca ctgctaatca agtaagtatg
     2161 ggagaaaaat ttagagtatg cgcg
//
LOCUS       DATAFW010000164         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_4527337, whole genome shotgun sequence.
ACCESSION   DATAFW010000164 DATAFW010000000
VERSION     DATAFW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4527337"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VHJ57_03750"
     CDS             <1..193
                     /locus_tag="VHJ57_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX2487678.1"
                     /translation="SRISKIVGLTQVVVSGEDVIVKGPSLEDVSQTASNIESSTKIKN
                     KDHRVFLDGVYLYSKEDGM"
     gene            375..478
                     /locus_tag="VHJ57_03755"
     tRNA            join(375..402,433..478)
                     /locus_tag="VHJ57_03755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Asn,seq:gtt)
     gene            670..1359
                     /locus_tag="VHJ57_03760"
     CDS             670..1359
                     /locus_tag="VHJ57_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX2487679.1"
                     /translation="MKAVILAGGLGKRLKPLTNDRPKPMIEVLNVPIIEWQLKWFKKY
                     NIKDIIICVGYLKENIIDYLGSGNKLGINIGYIVEDNPLGTGGALKNTSSLLNSSTED
                     GFFMTNGDILTNINPHKLCGEKISSIAVVPLRSPYGVVNIDNESNVIGFDEKPEIPDK
                     WINAGVYYFTKEVFDYLPDNGNIEVTALPAMANDQKLKGMRFNHNFWRSIDSHKDIDE
                     AAKELQSSNLV"
     gene            1356..2351
                     /locus_tag="VHJ57_03765"
     CDS             1356..2351
                     /locus_tag="VHJ57_03765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX2487680.1"
                     /translation="MNDISRIGYNIGTLLSIDNILRFAKKIEEKENTDSLWVPESWGR
                     EAFVSLGAVSQVTKRVRLGTAIISIHSRTPATVAMGISTLDILSNNRSVLGLGVSTPA
                     LVENWHGKKFDNPLEKLSEYIQCVKLIMKGSKVIFNGKYYKINDFKILFKPLRDDPPV
                     YTAAINKRMLDLSCELANGVLLYLRPKHELPKVVKYIKNKLQSKNNKKFEVCCAFITA
                     VSDQNPDAARLRAAKTLAFYVAIGEYYNRFLRENGFEIEVENINEEYQKNGLLHVHKF
                     ISDKMLTSLTISGTKEECIKSLREFTKSGISLPILQINPVGNNPENSIRELISTF"
BASE COUNT          908 a          298 c          420 g          802 t
ORIGIN      
        1 tagccgtata tctaaaattg ttggattaac acaagtcgta gtaagtggtg aagatgtcat
       61 agttaaagga cctagtttag aagatgtttc tcaaacagct tcaaatattg aatcttctac
      121 caaaataaaa aataaagatc acagagtatt tttggatggt gtttatctct attccaaaga
      181 agatggaatg taataataaa atattatttt actcttttct tctttattaa tcattaaatt
      241 taaactgtaa attttaacat gatataaatt aaaaattatt taaatttata gattttttta
      301 ttgtattgaa gtcatatcgt cttaattgca ataagaaaac atttttattt tgattaaaaa
      361 aatgatattt aaatgccgct gtagctcagc atggtagagc aaatttaaag cgaacttcgc
      421 tttagagctt agctggctgt taaccagtgg gtcatcagtt cgagtctgat cagcggcgtt
      481 tattttttta attcaagagt aatattattg attaggcaaa atgattgtct tgaagttatg
      541 ttacctcatt taatcattta ttaataaaga ttttaaaaat atcgtataag atatcatttc
      601 atttttctat aaaagaatat tatgtttaat tcattaatct ttaataatct gcctcgtgat
      661 tctattttga tgaaagctgt tatacttgcc ggtgggttag gaaaaagatt aaaaccttta
      721 acaaatgatc gacccaaacc tatgatagag gtattaaatg tacccataat cgaatggcaa
      781 cttaaatggt ttaagaaata taatatcaag gatataatta tatgtgtagg ttaccttaaa
      841 gaaaatatta tagattatct tggtagtggt aataaattgg gtattaatat aggatacata
      901 gtagaagata atcctctggg aactggcgga gcgctaaaaa atacatctag tcttttgaat
      961 tcatcaactg aagatggatt ttttatgaca aatggtgata tattaacaaa tattaatcca
     1021 cataaattat gtggagaaaa aatatcaagt attgcagttg ttcctctgag gagtccatat
     1081 ggagttgtta atattgataa tgaatcaaat gtaataggat ttgatgaaaa accggaaatt
     1141 cctgacaaat ggattaatgc aggtgtatat tactttacaa aagaggtatt tgattattta
     1201 cctgataatg ggaacataga agttactgca cttcctgcta tggctaatga tcaaaaatta
     1261 aaaggcatga gattcaatca taacttttgg agatcaatag attctcataa agatatagat
     1321 gaagcagcaa aggaacttca atcatcaaat ttagtatgaa tgatatttct agaataggat
     1381 ataatattgg tacgctttta tcaattgata atattttaag atttgcaaaa aaaatagaag
     1441 aaaaagaaaa tacagattct ttatgggttc cagaatcttg gggaagagaa gcctttgtat
     1501 ccttaggtgc tgtatcacag gtaacaaaaa gagttagact tggtacagca ataataagta
     1561 tacattctag aacccctgct acagtggcga tgggaatatc aacattagat atattatcca
     1621 ataatagatc agttttgggc ttaggagtaa gcactcctgc tttggtagaa aattggcatg
     1681 ggaaaaaatt tgataatcca cttgaaaaat taagcgaata catccaatgt gttaaattga
     1741 ttatgaaggg atctaaggta atttttaatg gaaaatatta taagattaat gactttaaaa
     1801 tattgttcaa accacttaga gatgatcctc cagtatatac tgctgcaatt aataaaagaa
     1861 tgttagatct ttcgtgtgaa ttagctaacg gtgttttgct ttatttaaga cctaaacacg
     1921 aattaccaaa agttgtcaaa tatataaaaa acaaattgca aagcaaaaat aataaaaaat
     1981 ttgaggtttg ttgtgccttt atcaccgcag tatccgatca gaaccccgat gcggctagat
     2041 taagagcagc aaagactcta gccttttatg tggcaatagg ggaatattat aatagatttt
     2101 tacgggaaaa tggttttgaa atagaagtag agaatataaa tgaagaatac caaaaaaatg
     2161 gtcttttaca tgttcataaa tttatttcag ataagatgct aacttcttta acaatatcag
     2221 gtactaaaga ggaatgcatc aagtctttaa gagaatttac taaaagtggg atatctttac
     2281 caatactcca aataaatcca gttggaaata atccagaaaa ttcaattagg gaattaatct
     2341 ctacatttta gttgagaaaa gtttctgttg ttggaggcgt cacaagtgaa tttaggaaag
     2401 aaacagatag agatatcacg catattgc
//
LOCUS       DATAFW010000165         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1889851, whole genome shotgun sequence.
ACCESSION   DATAFW010000165 DATAFW010000000
VERSION     DATAFW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889851"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1125)
                     /locus_tag="VHJ57_03770"
     CDS             complement(10..1125)
                     /locus_tag="VHJ57_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HEX2487681.1"
                     /translation="MRIWFDILTPKQIMFFKYFVDTLKEDDHEILCTGRDYREAKELA
                     KIKKLDIHTIGKHGGDNKFEKLYTSTERAFLLSSVINSFNPDLTVSFSSPEASRVAFG
                     LGIKHYIFNDSPHATAVAKLTVPLADRLFSPWVIPLREWTKLGFEKDRITQYKALDPI
                     VWIKKELQFNGTHEILKMQKKLKIDNTKQTILIRPEEIKASYITDKNVKSSIGLIDEV
                     VKQFSDEYNILILARYLDQVDFYKYRFEKRGGTKIIETVTDGTILLKLADLFVGAGGT
                     MSAEAALMGKPVISITPINFYVEKYLIKMGLIKKVKNSFTLTKYIKNILSQRGTTKNK
                     KIPNIKSDPNTIQEISLKIIAEMEDPIKIFKKFLFNP"
     gene            1302..2447
                     /locus_tag="VHJ57_03775"
     CDS             1302..2447
                     /locus_tag="VHJ57_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487682.1"
                     /translation="MSYSPNVAVYGLSTEGYNFASSIVSAGHKVSIIDESSKMGILLN
                     NDITRTYSTASDLIEDQPLLEAEPLDSVISSASYLFFFPKIRKIGHEVKIDISSKFID
                     AIKPINKDTSIVYPLPMGIAGNEENIDILEHTTGMTVGENLYYYYMPLNSGPILSTTT
                     ILGNFNSKRDNFLEGMLNKFTNQEINFVDIVSAELAYVSRILRHYSGIASVLEVCKFA
                     TGYGVDNRIVDNFHKDLYLDDITNGLHDLRIIQSSLHGSNPLVYLVNGTIKSIEGYSK
                     NLIDKVRYILKKRDLKASKTKISISWTLDVNEMRGDKIGVYTLLESRLRDYIGDVERR
                     GKNTLDNYPSDKTTIYIACSKTDFHLLSKNNSAETIIIKANPFCEVI"
     gene            complement(2585..>2846)
                     /locus_tag="VHJ57_03780"
     CDS             complement(2585..>2846)
                     /locus_tag="VHJ57_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX2487683.1"
                     /translation="GATFTNDPYPMSPKMIGVTVEDGAIIAARAVIKPGVTIGKNAVV
                     GMGSVVTKDVPSETVVIGNPARVKYSRKEYDEKKTIWEEHVI"
BASE COUNT          932 a          439 c          422 g         1053 t
ORIGIN      
        1 attataatgt tatggattaa agagaaattt tttgaatatt tttataggat cttccatttc
       61 tgctataatt tttaaagata tttcctgtat ggtattagga tcagatttta tattgggaat
      121 ttttttattt tttgttgttc cccgttgaga caggatattt tttatatatt tagttaaagt
      181 gaatgagttt ttgacctttt taattaaccc catttttata agatattttt caacataaaa
      241 attaataggt gttatagaaa taacaggctt acccattaat gccgcttcgg cgctcattgt
      301 ccctccagca cctacaaata aatctgcaag ttttagtaat atagtaccat cagttacagt
      361 ttctattatt tttgttccac cccttttttc aaatctatac ttgtaaaaat caacctgatc
      421 aaggtatcta gcaagaatta aaatattata ttcatcagag aattgtttaa ctacctcatc
      481 tattaaccca atcgaacttt ttacattctt atcagtaata tacgatgctt ttatctcttc
      541 tggtctaatt aaaatagtct gttttgtatt atcaattttt aatttttttt gcatttttaa
      601 aatctcgtgc gtgccattaa attgtagttc tttttttatc catactatcg gatcaagtgc
      661 tttatattgg gttatcctat ctttttcgaa accaagtttt gtccattctc gcaaaggaat
      721 tacccaagga gaaaataatc tatcagcaag cggaactgtt agtttagcta ctgcagtagc
      781 atgaggtgaa tcattaaata tataatgttt tattccgagt ccgaacgcta ctcttgaagc
      841 ctctggagat gagaaagaca ccgtcaaatc tggattaaat gaattaataa cacttgaaag
      901 aaggaaagct ctttcagtac ttgtatacaa tttttcaaat ttgttatctc caccatgttt
      961 acctattgtg tgaatatcta atttctttat ctttgctaat tctttcgctt ctctgtaatc
     1021 tcgtccagta cacagtattt catgatcatc ttcttttaac gtatctacaa aatatttaaa
     1081 aaacattatt tgtttgggag taagtatatc aaaccaaatt ctcaataatg taaatacgta
     1141 ataacatcat tgaatatact tttctttttt agttaatgag atggcattct gattaaattt
     1201 aaatgagttt caatcaaaag tgagatttat actgtagaat tattagctaa aagaaatgaa
     1261 taactttagt aaggagctct tttctatatc tgtttgttaa agtgtcctat tctccaaatg
     1321 ttgctgtcta tggattaagt acggaaggat ataactttgc ttcttctatt gtatctgcag
     1381 ggcacaaggt ctcaattatt gatgagtctt ccaaaatggg aatcttatta aataatgata
     1441 ttacaagaac atattctact gcatctgatc ttatcgaaga ccaaccatta cttgaggcag
     1501 aacctctaga tagcgtaata tcttcggcct cctatctctt cttttttccc aagataagaa
     1561 aaattggtca cgaagtaaaa attgatatat cttcaaaatt tatagatgct ataaaaccga
     1621 tcaataagga tacttctatt gtttatccac ttccgatggg aatagctggg aatgaagaaa
     1681 atatagatat tttagaacat acaacaggca tgacagttgg agagaattta tattactatt
     1741 atatgcctct taattctggt ccgattctgt caactactac tattttagga aacttcaact
     1801 ctaagagaga taattttttg gagggaatgt taaataaatt tactaaccag gaaataaatt
     1861 ttgttgatat agtgtctgcc gaattggctt atgtttctag aatcttacgt cattattcag
     1921 gaattgcaag tgtgctagaa gtttgcaaat ttgcaactgg atatggcgtt gataaccgaa
     1981 ttgtagataa ttttcacaaa gacctttatt tagatgatat aactaacgga ttacatgatt
     2041 taagaattat tcagtcatct ttacatggtt ctaatccact tgtatatctt gtaaatggta
     2101 ctatcaaaag tatcgaaggt tattctaaaa atcttatcga caaagtaaga tatattctca
     2161 aaaaacgaga tctcaaagca agtaaaacta agatttcaat ttcctggact ttggatgtca
     2221 atgagatgag aggagataaa ataggtgtat atacattgtt agaatcaagg ttgagagatt
     2281 atataggaga tgtggaaagg agaggaaaaa acacacttga caattatcca agtgataaaa
     2341 ctaccatata tattgcatgc tccaaaactg attttcattt actatcaaaa aataatagtg
     2401 cggaaacgat tataattaaa gccaatcctt tttgcgaagt aatataataa actaatatta
     2461 ttattataat aatagtaata aaaagaaaaa ttactggtta tattctgttt tagccttatt
     2521 taaagaatat tttttttatt agtaagatta aattggtatt taatttcttg ttaatatttt
     2581 tttgctaaat aacatgttct tcccaaattg tctttttttc atcatattcc tttctggaat
     2641 atttaactct ggcagggttg cctataacta ccgtttcaga tggcacatct tttgttacta
     2701 cagaacccat accaacaact gcattctttc caatagttac accaggtttt attactgctc
     2761 tagccgctat tattgctcca tcctctacag taactcctat catttttgga ctcatgggat
     2821 aaggatcatt ggtgaacgtt gcacca
//
LOCUS       DATAFW010000166         7980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5768280, whole genome shotgun sequence.
ACCESSION   DATAFW010000166 DATAFW010000000
VERSION     DATAFW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5768280"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VHJ57_03785"
     CDS             <1..1111
                     /locus_tag="VHJ57_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX2487684.1"
                     /translation="VAPPEEEVAPPEEEVAPPEEEVAPPEEEVAPPEEEVAPPEGEQQ
                     IPQGEQQQPSSPPAEEATDLEQLTGTGQEQQQQQSSPPATTTPPPAQKQQQPATITPL
                     PDQKQQQQQPGATDLEQLIAPDQKQQQQQPATTEVDTNVPSTNKKVVCNPAEQLLVTG
                     SSGNAVTNLQTILTQMGYDPKAVDGKFGPNTEKAVIQFQEKNDLVKDGKVGKNTWAKL
                     CALYNNQQPATTPNQKQQPATTPPPKVTSSPQYGGLLICKDCGKVNMNGISDKMTKIF
                     PNINAIYIKHTGLPGEITSAIRSADGKSLHDQGLAIDLRTKHLSNDVVKTMVKDLKSL
                     FEKDYDIIDEHNNPIGGVTKGPHIHIEYDPPKSKK"
     gene            complement(1221..1826)
                     /locus_tag="VHJ57_03790"
     CDS             complement(1221..1826)
                     /locus_tag="VHJ57_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX2487685.1"
                     /translation="MSNHLRQVWFNEPIKDSPAVKLIHLAYEKIERIILSFTIDLSRS
                     GKSYLDPSRPIKINGTIVQIPELERDSYSLTFVERKEIDVTNLLKYSPTGEDNTVEVY
                     FKSNQKLWSKKQAGKLSLVFTIYFPKEEAPKQQQESVSIKFMFCIFCRREIPDFSKFC
                     CHCGAKQEGGGREIKTCTVDNESLPQNARYCRKCGSAQPTL"
     gene            complement(2149..2550)
                     /locus_tag="VHJ57_03795"
     CDS             complement(2149..2550)
                     /locus_tag="VHJ57_03795"
                     /inference="COORDINATES: protein motif:HMM:NF039634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6046 domain-containing protein"
                     /protein_id="HEX2487686.1"
                     /translation="MSIVIDNLDLIKRVQDGVLEEIRNISYINIIDRRNIVELDIPGS
                     QGNIIQDMGFDAIKVELFGEIIGQNARTSIETLRDKYESNKPISFSSDITSIAEIIDV
                     IIEYLYLEEVAGSPFKYKYYISLREYVEERS"
     gene            2909..4288
                     /locus_tag="VHJ57_03800"
     CDS             2909..4288
                     /locus_tag="VHJ57_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX2487687.1"
                     /translation="MVRVIPGVEINVVKEIVPQQLYPAGVVGMIGTSNDGPIGTSVTV
                     TSYREFLEYFGPEEAGFTLHKEVKKAFLNGVFQVVATRVGGNNSYPASTVLKSNNSKK
                     KESLKLIARELGEKGNKIQVKVMRGATENTVRLEISAKNHLPEEFDNLSMLKDNDFYL
                     EKILNENSRIVTAQLLSDPSVENIPIPQEASLSGGQMGPPSIADYEQALEQLELETNI
                     DLVYVADVWDPEIHALVDAHCQNMSIGKEPKPLGPRLGIGTVAPKEEVNDIIKRTTRL
                     ASDRFVLVAPYGLAGSVAGLISKLNYYESPTFKALTGVASLERRYTPSEQMKLLTNGL
                     LPIDSVRGRGIIIVKGIATSKEQISVMRIADRSVRGIKNVADLFIGTLNNERGRLALK
                     ERITEFLIGMEREGSIVPSTDGSKPAYIVDVYSSQSDFAQGIVRTDIAVRPVRAMDYI
                     YATINVQAY"
     gene            4305..4715
                     /locus_tag="VHJ57_03805"
     CDS             4305..4715
                     /locus_tag="VHJ57_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487688.1"
                     /translation="MSSSPLLIAASTSKLTIDGKDVPGVQSLDFKISRNRQNIHSVSS
                     DERIGAYYGALYVQGSIKIRSAFPDLDKKLYETIPQLKHFQIMIELHPQGSDKMVKKI
                     TFDECYVEDKIFGMDATGVAITTYNFTSTRIREE"
     gene            4835..5230
                     /locus_tag="VHJ57_03810"
     CDS             4835..5230
                     /locus_tag="VHJ57_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487689.1"
                     /translation="MTKLTKEDILEGTRKRITVSLEEYGGKEVIVRPLSDSEVSKIIS
                     IIGPLSLSENEKKKDDMNLDIDIHKNIEALRLATEIGLVEPKLTKEDIQSMRFGVPEK
                     IGTIILENSGVSSPSSPSSSDALKKKSRK"
     gene            5504..7156
                     /locus_tag="VHJ57_03815"
     CDS             5504..7156
                     /locus_tag="VHJ57_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487690.1"
                     /translation="MSNFLKKNIINNNNMNLINIFSLSKINNTNIYNLNSFRKILKKF
                     LNQIKLAYISIGEGNSLKSNNLVNYQRTKNKIPILKDVFSHYNNFIDISKTLGSKNIS
                     VIDTIRSSGLFSATGTVGSSNNIYVHNVVSNGLFFYHDLVNLRKYVKDNFQTPFEDSL
                     INKVFSVLFSDVSNFAVPNNRFMRSQSPLSIFSSLHSLLEPSTTTFSSLHSLLEPSSY
                     KTTLSKFNSDTLHLKILNKIFSKTKNIIDPVTATADGQLSFFNNKIKFQERSYIFNNY
                     FKGTEKTLNDFDIVKKNFLFNSPSFKNLAKYDYNLSINLIPYEKLSNNIISKIRNNSK
                     ILENLDRIRNTTISTNFMKIELKKDIKKFVTDSNDLFDYNRILLSTIQKPHILLPTNN
                     KPTARGNPLRPPQLSTMQNQVENPRFQKYDEEEEESFRLTNINNMKINQTNDSAKLIK
                     KTKTIEDIFSAININDNIDKPTHPKLISLKYDIETILATELNRYSIHHNISTGSNKKI
                     INNIKNSNTGSIYKESYDYTINTLKKDLEDLFLIELRKHGVV"
     gene            7161..>7980
                     /locus_tag="VHJ57_03820"
     CDS             7161..>7980
                     /locus_tag="VHJ57_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487691.1"
                     /translation="MTIKIDDFELGKVIGDSSTGIKNEVRSVNVIKLGNKKRINYIPI
                     ASSYDKVTGNIGRFPVRIVVQGDIIGENAQDSVTMLRSKYKNNKPCNFVSDLSVLWGI
                     EKVIIEELRISNFSKFPQCFAYEMKLLEFNEPNSEAQQIQEEAKQQLAKAEDLAKQQL
                     AKAEDLAKQQLGKVEAMVKKTQDLAKQQLGKVEAMVNKIQEEAKQQLGKVEAMVNKIQ
                     EEAKQQLGKVEEEAKKIQEEAKQQLGKVEEEAKKIQEDAKQQLGKAEEEAKQQLG"
BASE COUNT         3083 a         1199 c         1276 g         2422 t
ORIGIN      
        1 agtagcacca ccagaagagg aagtagcacc accagaagag gaagtagcac caccagaaga
       61 ggaagtagca ccaccagaag aggaagtagc accaccagaa gaggaagtag caccaccaga
      121 aggagaacaa caaatacctc aaggagaaca gcaacaacca tcctcaccac cagcagaaga
      181 agcaactgac ctagaacaac taacaggcac aggccaagaa caacaacaac aacaatcctc
      241 accaccagca acaacaactc ctcctccagc ccaaaaacaa caacagccag caacaattac
      301 tcctcttcca gatcaaaaac aacaacaaca gcagccagga gctacagacc tagagcaact
      361 aatagctcca gatcaaaaac aacaacaaca acagccagca acaaccgaag tagatacaaa
      421 cgttccatcc acaaataaaa aagttgtttg taatcctgcc gagcagttat tggtgacagg
      481 ttcttctgga aatgccgtta cgaatcttca aactattctc acacaaatgg gttatgatcc
      541 aaaggcagta gacggaaaat tcggacctaa tactgaaaaa gcagttattc agttccaaga
      601 gaaaaatgat ttagtaaaag atggtaaagt tggaaaaaat acttgggcaa agttatgtgc
      661 attatataat aaccaacagc cagcaacaac tccaaatcaa aaacaacagc cagcaacaac
      721 tcctccaccc aaagttacaa gctctccaca atatggagga ttactaattt gtaaggattg
      781 cggtaaagtt aatatgaatg gtatatcaga taaaatgacc aaaatttttc caaatataaa
      841 tgcgatatac ataaaacaca ccggattgcc tggtgaaata acatcagcca ttagatccgc
      901 cgatggaaaa tcattgcatg atcaaggatt agctattgat ttacgaacta agcatttatc
      961 caatgacgta gtaaaaacga tggtcaagga tttaaaaagt ctttttgaaa aagattatga
     1021 tattatagac gaacataata acccaattgg aggtgtaaca aaaggacctc atattcacat
     1081 tgaatacgat cctccaaaat ctaagaaata aattttagaa aataatagat tttgtttatt
     1141 ttatctgtta tatgttctaa ttgattggtt gattgagaga atatatatgc aggaagggaa
     1201 attaatgaga atatattttg ttataaagta ggctgagcac taccacattt tctacaatac
     1261 cttgcatttt gaggaagact ctcgttatct actgtacatg ttttaatttc tctaccgcca
     1321 ccttcttgtt ttgccccaca atgacaacaa aattttgaaa agtctggaat ttctcttcta
     1381 cagaatatac aaaacataaa tttaatggat actgattctt gttgttgttt aggagcctcc
     1441 tcttttggaa aatatatagt aaaaaccaaa cttaattttc cggcctgttt ttttgaccat
     1501 aacttttgat tcgatttaaa atagacctct actgtgttat cttctcctgt aggactatac
     1561 tttaatagat ttgttacatc tatttctttt ctctcaacaa aagtgagaga ataagaatct
     1621 ctttccagtt caggaatttg tactattgta ccattgattt ttataggtcg cgaaggatcc
     1681 aaatagcttt tacctgaacg tgaaagatct attgtaaatg ataaaataat gcgttctatt
     1741 ttctcatatg ctaagtgaat taatttcaca gcaggagaat cttttattgg ttcattaaac
     1801 catacttgac gaagatgatt tgacattgga cactatgcta tttgttatat atatataatt
     1861 aatatataat ttcttcttta ttgttaacta atagaacaat aattttgatt ttgtatttct
     1921 gaataatctg gcttttattt attttttgta taggtatttt atactctttc tcaaattatt
     1981 aatacaaaag aattgtaagt ttttactagt gataatagca gtcgattact ttttatgatt
     2041 ttaataacct tattttaaaa gatgtaatat atgtttaaga acttatactg tattggtagt
     2101 tagtactaga gaatgcaatc aataaatttt ttacattata catttacttt aagatctttc
     2161 ttcaacatat tctcttaaag atatataata tttatattta aatggagacc cagcaacttc
     2221 ttcaagatat aaatattcta ttataacatc aataatctct gcaatagatg ttatatcaga
     2281 agaaaaggat atgggtttgt tagattcata tttgtctcta agagtttcta tacttgttct
     2341 agcattttga ccaatgatct caccaaataa ttctaccttg atcgcatcaa atcccatatc
     2401 ttgaattata ttcccttgag aaccaggaat atctaattct acaatattcc ttctatctat
     2461 tatatttata taggaaatat ttcgtatttc ctctaaaaca ccatcttgta ctctttttat
     2521 aaggtctaga ttatctataa ctattgacat ttatttgtat tgcagttata aaaaagcatt
     2581 ttattattta tcctttttct aaaaaattat tataatatag attaaaatta gtatttctct
     2641 atattagttt ctaaatatcg acaacataat aacagattaa agcgagagtt actattatag
     2701 attgactgtt aattatatta tataacaaaa taattgttct tcatagagtt tgggaagacg
     2761 aaattatcat tataaatatg cttttttaaa agactatatg tattagtcaa ttcttattaa
     2821 ttcaaccaaa aaatcaaaat aatataatat tttattcaat aatggcaaga aaattgttat
     2881 atattaccat ttaattaaag atttgtacat ggtaagagta atacctggag tagaaattaa
     2941 cgttgttaaa gaaattgtac ctcaacaatt gtatcctgct ggtgtagtcg gcatgatagg
     3001 tacttcaaat gatggtccta tcgggacatc agtcactgtt acaagttata gagaatttct
     3061 cgaatatttt ggaccagaag aagcaggatt tacgcttcat aaagaggtaa aaaaagcatt
     3121 ccttaatggg gtttttcaag ttgttgccac aagggttgga gggaataata gttatcctgc
     3181 ttcaacagta ctaaaaagta ataatagcaa gaaaaaagag tcactaaaat taatagctag
     3241 agaattagga gagaagggta acaaaataca ggttaaagtt atgagaggag caactgagaa
     3301 tactgtccgt ctagaaattt ctgctaaaaa tcatcttccg gaagaatttg acaatttatc
     3361 aatgttaaag gataatgact tttatttaga gaagatttta aatgaaaatt ctcgcattgt
     3421 gactgcacaa ttgttgagcg acccatcagt agaaaatatt ccaattcctc aagaagcttc
     3481 acttagtgga ggacagatgg gaccaccttc tatagcagat tatgaacaag cattggaaca
     3541 attggagtta gaaacaaaca tagatttagt atatgttgct gatgtttggg atcctgaaat
     3601 acatgctctt gttgatgctc attgtcaaaa tatgagtata gggaaagaac caaaacctct
     3661 tggacctaga ctaggcatag gtactgtggc accaaaggag gaagttaatg atattataaa
     3721 gagaacaaca cggctagcta gcgatcgatt tgtgctagta gctccttatg ggttagctgg
     3781 atctgttgcc ggattgataa gtaaactcaa ttattatgaa tcccctacat tcaaagcatt
     3841 aacaggtgta gccagccttg aaagaagata tactccttct gaacaaatga aactcttaac
     3901 aaatggacta ttacctattg attcagttag aggaagaggt attatcatag tcaagggtat
     3961 cgccacaagt aaggaacaga ttagtgttat gcgaatagcg gatcgatctg tacgtgggat
     4021 caaaaatgta gcggatcttt tcattgggac acttaataat gaacgtggca gattagcatt
     4081 aaaagagaga ataactgaat ttcttatagg gatggaaaga gaaggatcaa ttgtacccag
     4141 tactgatgga tctaagccag catatattgt ggatgtttat tcatctcagt ctgattttgc
     4201 acagggtata gtgagaacgg atatagcagt tagaccagtt cgtgcaatgg attatattta
     4261 tgctactata aatgtacagg catattaaaa gaggagtttg aaaaatgtca tcatcaccat
     4321 tattaattgc agcaagcaca agtaagctaa ctatagacgg taaagatgtt ccgggagtac
     4381 aatcactcga ttttaaaatt tccagaaata ggcaaaatat acattcagta agttctgatg
     4441 aaagaattgg agcttattat ggtgcattat atgtccaagg ttccataaaa ataagatcag
     4501 cattccctga tctagataag aaactatatg aaaccatacc acaactcaaa cattttcaaa
     4561 taatgattga attacatcct caaggttctg ataaaatggt caaaaaaatt acctttgatg
     4621 aatgctatgt tgaagataag atatttggca tggatgctac tggagtagcc ataactacat
     4681 ataattttac ttctaccaga ataagggaag agtagagaga aaccagaaca aaatacgata
     4741 aagtaatgaa ggaaggaaag aaggctggaa agaaaaggag taaagtgagg aatattactt
     4801 tagtcctatt gaaattatag atgtgaacta ttatatgaca aaattgacca aagaggatat
     4861 tttggaaggt actagaaaga ggattacagt ctctctcgaa gaatatggag gtaaagaagt
     4921 tatagttagg cctctctctg attctgaagt ttctaaaata atatctataa tcggccctct
     4981 ttccctttca gaaaatgaaa agaaaaaaga tgatatgaat ctagatatag atatacataa
     5041 aaatatagag gctttacgat tagcaacgga gattggtctt gttgaaccaa aattaactaa
     5101 ggaagatatt caatctatga gatttggtgt tccagaaaaa attggaacca taatattgga
     5161 aaatagtgga gtgtcttctc cttcttcccc ttcgtcttct gatgctttaa aaaaaaaatc
     5221 caggaaatag aatcttttgt acaaactcaa gaaggaagag aactctctat aatttgtcta
     5281 gatcttaact ataaattagc caattttgct ggagatctta ctagagatca aattaatttt
     5341 cttatttact ccttaaaatt gagacgtcaa gccgaagaaa catcatattt agcagaccaa
     5401 ggtataactc gtatagtttt caaccaagag gaagggtgat ataatatatt tgaagatgtt
     5461 gtatattggt aactgtgaga gtctagtaaa atattcttat gaaatgtcca attttttaaa
     5521 aaaaaatatt attaataata ataatatgaa tttaattaat attttttcac tttcaaaaat
     5581 taacaataca aatatctata acttaaactc tttccgaaaa atattaaaaa aatttttaaa
     5641 ccagattaaa ttagcttaca taagtatagg agaagggaat tctcttaaaa gtaataattt
     5701 agttaattat caaagaacca aaaataaaat acctatactt aaagatgttt tcagtcatta
     5761 taacaatttt attgatattt caaaaacatt aggatcaaaa aatatttcag ttattgatac
     5821 tattagatca agtggtttat tttcagcaac tggaacagtt ggaagtagta acaatattta
     5881 tgttcataat gttgtaagca atgggctctt cttttatcat gatcttgtca atttacggaa
     5941 atatgtaaaa gataactttc aaactccatt tgaggattca ttaattaata aagttttttc
     6001 agttcttttt tctgatgtgt ccaattttgc tgtacctaat aataggttta tgcgatcaca
     6061 atcaccatta tccatcttta gctcactgca ttcattgcta gaaccatcaa ccaccacctt
     6121 tagctcactg cattcattgc tagaaccatc atcttacaaa acaactttat caaaatttaa
     6181 ttctgatact ttacatttga aaatattgaa caaaattttt tctaaaacaa aaaatataat
     6241 cgatcctgtt actgctactg ctgatggtca attatctttt tttaataata aaataaaatt
     6301 tcaagaaaga agttatatct ttaataacta ttttaaaggt acagaaaaaa cattaaacga
     6361 ttttgacatt gtgaaaaaga acttcttatt taactctcct tcatttaaaa atttagcaaa
     6421 atatgattat aatctatcta taaatttgat accatatgaa aaattatcaa ataatattat
     6481 ttcgaaaata cgtaataata gtaaaatatt agaaaatcta gatcggataa gaaatactac
     6541 tatttctact aattttatga aaatagaatt aaaaaaagat attaaaaaat tcgttactga
     6601 ttcaaatgat ttatttgact ataataggat cttattgtct actattcaaa agccgcatat
     6661 attattgcca acaaataaca aaccaacagc aagaggaaat ccattacggc caccacaatt
     6721 atcaactatg caaaatcaag ttgaaaaccc aagattccaa aaatatgacg aggaggagga
     6781 ggaaagtttt agattaacaa atatcaataa tatgaaaata aatcagacaa atgattcagc
     6841 aaaacttata aaaaaaacaa aaactattga ggatattttc tctgctatca acataaatga
     6901 taatatagat aaacctacac atcctaaatt gatttcatta aaatatgata ttgaaacaat
     6961 tcttgcaacc gaattgaata gatattctat tcatcataac atctctactg gatcaaataa
     7021 aaaaattata aataatatta aaaatagtaa taccggctca atatacaaag aaagctacga
     7081 ttacactatt aatactttaa aaaaagactt ggaagatctc ttccttatag aattaagaaa
     7141 acatggggta gtctaatacc ttgactatta agattgatga ttttgaatta ggaaaagtaa
     7201 taggtgacag tagcacagga ataaaaaatg aagtaagaag tgtcaacgtt ataaaacttg
     7261 gtaacaaaaa acgtataaac tatattccaa ttgctagttc ctatgataaa gtaacaggta
     7321 atataggtag atttccagta cgtattgtag ttcaaggaga tatcattggc gagaacgcac
     7381 aagacagtgt aaccatgcta cgatcaaagt ataaaaataa taaaccatgt aattttgttt
     7441 ctgatttgag tgtattatgg ggtattgaaa aagtaataat tgaagaatta cgaatttcta
     7501 atttctctaa atttccacag tgttttgctt atgaaatgaa attactagaa ttcaatgaac
     7561 caaatagcga agcacaacag attcaagaag aagcaaaaca acaattagca aaagctgaag
     7621 atctagcaaa acaacaatta gcaaaagctg aagatctggc aaaacagcaa ttaggtaaag
     7681 ttgaggctat ggtaaagaag actcaagatc tggcaaaaca gcaattagga aaagttgagg
     7741 ctatggtaaa taagattcaa gaagaggcaa aacagcaatt aggaaaagtt gaggctatgg
     7801 taaataagat tcaagaagaa gcaaaacaac aattaggaaa agttgaagaa gaggcaaaga
     7861 agatccaaga agaggcaaaa cagcaattag gaaaagttga agaagaggca aagaagatcc
     7921 aagaagatgc caaacaacaa ttaggaaaag cggaagaaga agcaaaacaa caattaggaa
//
LOCUS       DATAFW010000167         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6300494, whole genome shotgun sequence.
ACCESSION   DATAFW010000167 DATAFW010000000
VERSION     DATAFW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6300494"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /gene="uppS"
                     /locus_tag="VHJ57_03825"
     CDS             <1..316
                     /gene="uppS"
                     /locus_tag="VHJ57_03825"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX2487692.1"
                     /translation="AYGGQQELVDAIKKIAALVSAGKLKVNDINKKIIESCLYTSHLP
                     QSDPDLILRTSGEKRLSGFLLWQSAYSELVFMDVFWPEFRKIDFMRAIRTYQNRIRRF
                     GK"
     gene            358..513
                     /locus_tag="VHJ57_03830"
     CDS             358..513
                     /locus_tag="VHJ57_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487693.1"
                     /translation="MHEHGFYDPHLKKWYCNYFMDEEEWLDIHDYLPIQFIEHSSDNE
                     SSDNSIK"
     gene            537..953
                     /locus_tag="VHJ57_03835"
     CDS             537..953
                     /locus_tag="VHJ57_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX2487694.1"
                     /translation="MRTEFSCGALLYCIDNQQIIFLLLHYFSGHWDFPKGNKERGENY
                     IETIRREIKEETGISDLNFIDGFVKEISYKYLRQNQLISKKVVYFLARTNSKDVVLSS
                     EHTDFVWDHYENALKRLTYKKSKEILTEGYKFLKKL"
     gene            1030..1386
                     /locus_tag="VHJ57_03840"
     CDS             1030..1386
                     /locus_tag="VHJ57_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX2487695.1"
                     /translation="MFLNSKYYTIFNTDLQLSNLETKIFLLIINQGMLKIDRISLELN
                     LDKSQCREIIESLIKKNMIIEYSKNLFESYHPRFAIINRYKRLCLEKNIPWKKNTVID
                     NLAVILEQPFEAARTK"
     gene            1411..1824
                     /locus_tag="VHJ57_03845"
     CDS             1411..1824
                     /locus_tag="VHJ57_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487696.1"
                     /translation="MSENQSKTDDKIVYFGVININEKGKTIGAIDVWRSVITKELFCE
                     EKRLGILDIAEYIGMPKLEKGNKWAVAINRNRIGKDRWKLIKILEEGTFKFYDTNDET
                     IINTNVKNYQIIDNDWWNFLVESNINRSIEITKEL"
     gene            1828..2127
                     /locus_tag="VHJ57_03850"
     CDS             1828..2127
                     /locus_tag="VHJ57_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487697.1"
                     /translation="MYRLGDNDELKVDVFINNSSGGEVASKLTGYFVVKNKSFRFSAI
                     AFGRIGGHNISIKLSTSSINKLTKMEIDPEKLQLTIQRKLIEGDVNLPQNMKLPK"
     gene            complement(2174..2983)
                     /locus_tag="VHJ57_03855"
     CDS             complement(2174..2983)
                     /locus_tag="VHJ57_03855"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX2487698.1"
                     /translation="MIKNLKYSSTIGIIGGTGIYDSNIFNVIDEIKINTPYGETSDRI
                     TICTYQEKKIAFIPRHGKNHRLPPHKVNYRANIWALNELGVKVILAPSAVGSLKSQYG
                     PGDLVLPDQYIDFTKKREYTFYDGPVVCHISQSDPFCPNLRETIITDLKYLGFKFHPL
                     GTYICIEGPRFSTKAESNYYRTLLKGDIIGMTLIPECILAKEMKMCYVSISTVTDYDV
                     WAEITVNSKEIIETLNKNVDKTKKLLLTVIPKIKEDRNLCNCSNSLENALV"
     gene            complement(2976..>3269)
                     /locus_tag="VHJ57_03860"
     CDS             complement(2976..>3269)
                     /locus_tag="VHJ57_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX2487699.1"
                     /translation="RKVGKLPGKTMKKKYNIEYGSSIIEIQEEAIKKGQNILIADDLI
                     ATGGTANATAELIEEMGAKVFGFAVIIELVYLKGAEILRNKGYEVRSLVKYYD"
BASE COUNT         1197 a          446 c          507 g         1119 t
ORIGIN      
        1 ggcatatggt ggacaacaag agttagtaga tgctataaaa aaaatagctg ctttggttag
       61 tgctggcaaa ttaaaagtta atgatattaa taaaaagatc attgaatcat gtctctatac
      121 atctcatctt ccacaatcgg acccggacct aattcttcga acttctggtg aaaaaagatt
      181 aagtggtttt ttactttggc aaagtgctta tagcgaattg gtatttatgg atgttttttg
      241 gccagagttc aggaaaattg attttatgcg tgctataagg acttaccaga atcgtatcag
      301 gagatttgga aaataaacta ttaactaaaa ataacccatt aattctctct ttatcaaatg
      361 catgaacatg ggttttatga tcctcatttg aaaaaatggt attgtaacta ttttatggat
      421 gaggaggaat ggttggatat acatgattac ttaccgatac aattcataga gcattcgtca
      481 gataatgaat cttcagataa tagtattaaa taacttgaag ttttgatggt gattttttga
      541 gaaccgaatt ttcttgtgga gcattattgt attgtatcga taatcaacaa ataatatttc
      601 ttctattaca ttatttctct ggacattggg attttccaaa aggaaataaa gaaaggggag
      661 aaaattatat agaaactata agaagggaaa ttaaagaaga gacaggtatt agcgacctta
      721 attttatcga tggttttgta aaagaaattt cctataaata cctacgtcag aatcagttga
      781 tctccaaaaa agtagtctat tttttagcaa gaaccaattc caaagatgtt gtgttgtcat
      841 ccgagcatac agatttcgta tgggaccact atgaaaacgc tttaaaaaga cttacttata
      901 aaaaatctaa agaaatcctc actgaaggtt ataaattttt aaaaaaatta taattcatat
      961 ttttgtaaat ctaaaaatct aatgttagaa taagaaatat ttattttaga ttaaggagaa
     1021 attcttctat tgtttttaaa ttctaaatat tatactatat ttaatacaga ccttcaactt
     1081 tcgaacctag aaactaaaat atttcttctt ataataaacc aaggtatgtt gaagattgac
     1141 cggatatcat tagagttgaa tttagataaa tcccagtgta gagaaattat agaatcatta
     1201 attaaaaaaa atatgataat tgaatattcg aaaaatttat ttgaatccta ccatcctaga
     1261 tttgctataa taaatagata taagagattg tgtttagaaa agaatattcc ctggaaaaaa
     1321 aataccgtta ttgataatct tgcagtcata ctagaacaac catttgaggc tgcaagaact
     1381 aaataaggag aaataaaaat ataattttct atgagtgaaa atcagtctaa aacagatgac
     1441 aagatagttt attttggtgt cattaatata aatgagaagg gtaaaactat aggcgcaata
     1501 gatgtctgga ggagcgtcat cactaaggaa cttttctgtg aagaaaaaag attaggaatt
     1561 ttagatatag cagaatatat tggtatgccg aaattagaaa aaggaaacaa atgggcagtt
     1621 gccataaata gaaatcgtat tggtaaagat agatggaaac taattaaaat tttagaagaa
     1681 ggaactttca aattctatga taccaatgat gagacaatta ttaatacaaa tgtaaaaaat
     1741 taccaaataa tcgataatga ttggtggaat tttcttgttg aaagtaacat aaatcgaagt
     1801 atcgaaatta caaaagagtt ataagccatg tatagattgg gagataacga cgaacttaaa
     1861 gtagatgtct ttataaataa ttcgtctgga ggagaagttg caagtaaatt gacaggttat
     1921 tttgttgtta aaaataaaag tttcagattc tctgctattg catttgggag aattggagga
     1981 cataatatca gcataaaatt atcgacttca tccattaaca aattaacaaa aatggaaatt
     2041 gatccagaaa aactgcaatt gactatacaa agaaagttaa ttgaagggga tgtcaatctt
     2101 cctcaaaata tgaaattacc taaataaatg atatgttatt tataattaat aaaaaaataa
     2161 aatcaacgag ttattatact aatgcatttt ctaacgagtt agaacaatta cataaatttc
     2221 tgtcttcttt tattttgggt ataactgtta gtaacaattt tttagtttta tcaacatttt
     2281 tatttaatgt ttcaattatt tcctttgaat ttacagtgat ttcagcccaa acatcataat
     2341 cagttaccgt agaaatggat acataacaca tcttcatttc tttagctaat atgcattcgg
     2401 gaattaaggt cattcctatt atatcacctt taagtaatgt tctatagtaa ttagattcag
     2461 ctttagtaga aaatcttgga ccttctatac aaatataggt tcctaatgga tgaaacttaa
     2521 aacctaaata ttttaaatct gttataatag tttctctcaa attgggacaa aatggatcag
     2581 attgagaaat atggcaaact actggaccat cataaaatgt atattctctt ttttttgtga
     2641 aatctatata ctgatcaggt aatactagat cccctggtcc atattgtgat ttcagacttc
     2701 ctacagccga tggtgcgagt attactttaa caccaagttc attgagagcc caaatatttg
     2761 ctcggtagtt aactttgtga ggaggaagtc tatgattttt tccatgacga ggaatgaatg
     2821 ctattttttt ctcttgatat gtacaaattg ttattctatc agatgtctct ccatacggag
     2881 tattaatttt aatttcatct atgacgttaa aaatattaga gtcataaata cctgttccac
     2941 caataatgcc tatagttgaa ctatatttta aatttttaat cataatattt caccaatgaa
     3001 cgaacctcgt aacctttatt tcttaaaatt tctgctcctt ttagatatac caattctata
     3061 atcactgcaa aaccaaaaac ttttgctccc atctcctcaa ttaattcagc agttgcattg
     3121 gcagtgccac ctgtagcaat taggtcatca gcaattaaaa tattttgacc tttttttatt
     3181 gcctcctctt gtatttcaat tattgaacta ccatattcaa tattatactt ttttttcatt
     3241 gttttaccgg gaagcttacc aacctttct
//
LOCUS       DATAFW010000168         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_2423808, whole genome shotgun sequence.
ACCESSION   DATAFW010000168 DATAFW010000000
VERSION     DATAFW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423808"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VHJ57_03865"
     CDS             complement(<1..469)
                     /locus_tag="VHJ57_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487700.1"
                     /translation="MNTDFWNNIYKYKEYGFEPLNWLSNFSNEIDPDLLESTLNKIKG
                     TNWKIIPDWFDWFYKSERKNPEVTRRIYNLSDLDIQQSETGIKKSIAIIQLNKEIVDN
                     QEKLLHLLLKLINSIKSKCVTKKISSINTSSKRQFALIDFIETRRGNKVGYIAA"
     gene            856..1671
                     /locus_tag="VHJ57_03870"
     CDS             856..1671
                     /locus_tag="VHJ57_03870"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX2487701.1"
                     /translation="MIESNSEHSIKDINFTFLNNANQRSKHNYIINDLADRFEKLSAT
                     DILKWSIEKFGTKIALASSFGAEDVAIIDMMVKIDKSKTKIVTLDTGRLNQETYDVMD
                     KIRKKYDISIESYFPNSQQVEEMVYKKGFNLMYESLENRKLCCKIRKVKPLNRALASL
                     DGWITGLRREQAVTRFNIKKIEIDPTHNDILKINPIADWSEDMVWEYIKENQVPYNKL
                     HDMGFPSIGCEPCTRAITKGENPRSGRWWWEEASQKECGLHIDPNITKKSELL"
     gene            1900..3072
                     /gene="sat"
                     /locus_tag="VHJ57_03875"
     CDS             1900..3072
                     /gene="sat"
                     /locus_tag="VHJ57_03875"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX2487702.1"
                     /translation="MENISIQNNIIKKIPEPHGGRLVNRIFESKNINTKDMFSIEIDD
                     NLRNDVENIGDGIFSPLEGFIGKVDFESILKFGRLKNGLPWTIPIILDVEEPIAKKIQ
                     DYREISLKNKGEHFATLYFDEFYSYNKLEMVRALYQSENEQHPGVSKTFKMKEYLVSG
                     RIDVYKKINKFKLRNYRLEPIKTREEIKKRGWKTIVGFQTRNIPHIAHEMLQKAALNI
                     YDGLFLNPLIGKKKIGDFKDELILESYLKLIENYYPQDRVMFVTLHTEMRYAGPKEAI
                     HHAIMRKNFGCSHFIVGRDHAGVGDYYHPFAAHEIFKEYPDIGIEPIFFPSFYYCKKC
                     LGYANERNCPHGLEYKEELSGTKMRKMVTSGEMPGKHLMRPEIADVIIKFQDPFVS"
     gene            3241..3921
                     /locus_tag="VHJ57_03880"
     CDS             3241..3921
                     /locus_tag="VHJ57_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487703.1"
                     /translation="MIFFSNNFLYAQNEFESPAETLNVTKSKSHESFVVPFSASNTDR
                     KNPLIHTFETSKDDSWILGIDNNLTYNNSSEAKTIIKLMENKPSEKFIELAMFEQNEK
                     FWVAVNTNESGYIRVHERDTDGWSRDEPLMVGHANNQGLTITNGKRIVIDRLSLDGFN
                     LGSIAIYGKDDTDSPINTLDGYLSFDLIFGNPRDSPLYFLPIIMLVLVGGIIFYLLKI
                     KKRDRENE"
     gene            complement(3932..4648)
                     /gene="nth"
                     /locus_tag="VHJ57_03885"
     CDS             complement(3932..4648)
                     /gene="nth"
                     /locus_tag="VHJ57_03885"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX2487704.1"
                     /translation="MKKKSSIKLQNNNRPQELIKIENILDKMETTLNYFENLSRLTAL
                     KELQMEEDNDPFKILIGTILSARSRDENTSKIVRKLFQKYKNALELSNADLNDVKSII
                     HSIGFYNTKSERIKQASKIIVEKFHGIVPSELEDLLSLPGVGRKTANCVLVYAFNKPA
                     IPVDTHVHRISNRLGIVKTQDVKKTEEELIKIVDKKYWLILNNIFVIYGQNICIPIRP
                     KCQICSLKGICNFYINQKKI"
BASE COUNT         1762 a          663 c          739 g         1755 t
ORIGIN      
        1 tagcagcgat gtatcctact ttatttcctc ttcttgtttc aataaagtct atcaacgcaa
       61 attgcctttt tgaagaggta tttattgaac taattttttt ggttacacat tttgatttaa
      121 tactattaat taactttaac aaaagatgga gtagtttttc ttgattgtcc acaatttcct
      181 tattcaattg gataatagct attgatttct tgatacctgt ctcactttgc tgaatatcca
      241 agtctgataa attataaatt cttctagtaa cttcaggatt ctttctttca cttttataga
      301 accaatcaaa ccaatctggt ataattttcc aatttgttcc tttaatttta tttagagtag
      361 attctaacaa atctggatca atttcattag aaaaattgga aagccaattt agtggctcaa
      421 aaccatattc tttgtacttg taaatattat tccaaaaatc tgtattcaat ataatctatg
      481 taatgaatta tagtaatgat attaaaatat agatattttt tatcaatttt attaattatt
      541 aactctattt ttatcttaca taagtcaata attttatcag taaaataggt ttttagaaca
      601 agatgtcaaa atatcccaat gcgctaaggc cgttgttatt acactttctt taatttcatt
      661 tctagatgat aaagactccc aaagctattt atttatttaa taggtcgtca tcacgataag
      721 acttaattat cctgtatgaa aaaactagtg acaaattatc cactaaatat tcccaagata
      781 aagcatgtta taatttgata tcttactttt gcaactcaaa ataaatatat acatttttaa
      841 ataaaatata ttattgtgat agaatcgaat tctgagcata gtataaagga tataaatttt
      901 acatttttaa acaatgcaaa tcaaagatcg aagcataatt atattataaa tgatttggcc
      961 gacagatttg aaaaattatc tgcaacagat attcttaaat ggtcaattga aaagtttgga
     1021 actaaaattg cattggcatc aagttttggt gctgaagatg ttgcaattat tgatatgatg
     1081 gtcaaaatag ataaaagtaa aacgaaaata gtgaccttgg atacaggtag attaaaccaa
     1141 gaaacatatg atgttatgga taaaatacgt aaaaaatatg atatttctat agaatcatat
     1201 tttccaaatt cacagcaagt tgaagagatg gtttataaaa aagggtttaa tctgatgtat
     1261 gaaagtctag aaaatagaaa attatgctgt aaaataagaa aggtaaagcc acttaatcga
     1321 gctcttgcct ctcttgatgg atggataacc ggtctaagaa gagaacaagc agttacccgt
     1381 ttcaatataa aaaaaattga gattgatcca actcataatg atattctaaa gattaatccc
     1441 attgctgatt ggagtgaaga tatggtttgg gaatacataa aagaaaatca ggttccatat
     1501 aataaattac atgatatggg attcccaagt attggttgtg aaccatgtac tcgtgctatt
     1561 acaaaaggag aaaatcctag atctggcaga tggtggtggg aagaagcatc tcaaaaggaa
     1621 tgtggacttc acattgaccc gaatataaca aaaaaatctg aacttttata aagatatcaa
     1681 taattcttgt acatgtaaga gacagtagat atatctgcaa taatcacttt tgattttaca
     1741 catacacagt agatctcaca ttggttgcaa gacattttat ccacaattag cggttattat
     1801 attatgtaca agttatatta tcatactaga ggtaattgca acattataat ggtatttgaa
     1861 aagagttaat aatacaatta atggtctaaa gtataaaaat tggagaatat ttctattcaa
     1921 aataatatta taaaaaaaat tccagagcct catggcggtc gacttgtaaa tagaattttt
     1981 gaatcaaaaa atatcaatac caaagatatg ttttccattg aaattgatga taatcttaga
     2041 aatgatgttg aaaatatcgg agatggtatt tttagtccgt tagaaggttt tattggaaaa
     2101 gttgattttg aaagtatttt aaaattcgga agattaaaaa atggattgcc atggacaatt
     2161 cctataattt tagatgtaga agaaccaatt gcaaaaaaaa tacaagatta tcgagaaatc
     2221 tcactaaaaa ataaaggtga acattttgca actctatatt ttgatgagtt ttattcgtat
     2281 aataaattag aaatggtaag ggcattatac caatctgaga atgaacaaca tcctggagta
     2341 tctaagacct ttaaaatgaa agaatattta gtttctggaa gaatcgatgt ctataaaaaa
     2401 ataaataaat tcaaattacg aaattataga ttagaaccaa taaagactag agaagagatt
     2461 aaaaaacgag gatggaagac catagtggga tttcaaacca gaaatattcc ccacatagca
     2521 catgaaatgc ttcaaaaagc ggctttaaat atctatgatg gattatttct caaccctttg
     2581 atcggaaaaa agaaaatagg tgattttaaa gacgaattga tacttgaatc atatctaaag
     2641 ttaatagaaa attattatcc acaagataga gtgatgtttg ttactcttca tacagaaatg
     2701 agatatgcag gaccaaaaga ggctatccat catgcaatta tgagaaaaaa ctttggctgt
     2761 tctcatttta tagtaggaag agaccatgct ggtgtaggtg attattatca tccatttgca
     2821 gctcacgaaa tctttaaaga atatcctgac attggaattg aaccaatatt cttcccatca
     2881 ttctattact gtaaaaaatg cttaggatat gctaatgaga gaaattgtcc tcatggttta
     2941 gagtataaag aagaattatc cgggacaaaa atgagaaaga tggttacttc tggagagatg
     3001 ccaggaaaac atttaatgag gccagaaatc gcagatgtaa ttataaaatt tcaagatcca
     3061 tttgtttctt aaaattttgc tggttggatt ctataaacta attttattgt tctcatgttt
     3121 tttaatcttt gatttacgtt catattactc taaataattt tatataaaat gcactcaatg
     3181 catagaattt ctttatttta ttttttgaaa ttgtcttcat ttattggagc tttcatctat
     3241 atgatatttt tttcaaataa ttttttatat gctcaaaatg aatttgaatc tccagcagaa
     3301 acgcttaatg ttacaaaatc aaaaagtcat gaaagctttg ttgttccctt tagcgcatct
     3361 aatacagacc gtaaaaatcc actgattcat acttttgaga cttccaaaga tgatagttgg
     3421 attcttggca ttgataataa tctgacctac aacaattctt ctgaagcaaa aacaattatt
     3481 aaattgatgg aaaataagcc tagcgaaaaa ttcattgaat tagccatgtt tgaacaaaat
     3541 gaaaagttct gggttgctgt taatacaaat gaatcaggat acataagagt ccatgaacga
     3601 gataccgatg gatggtctcg tgatgaacct cttatggtag gacatgctaa taatcaaggt
     3661 ttaacaatta ctaatgggaa aagaatcgta attgacaggc tatccttaga tggttttaac
     3721 ttaggttcta ttgctatata tggaaaagat gatactgatt ctccgataaa tactttggat
     3781 ggctatcttt cctttgatct tatattcggt aatcctagag attctccatt atattttcta
     3841 cctatcatta tgttagtttt agttggaggg ataatattct accttctcaa aattaagaaa
     3901 agagaccgtg agaatgaata aataaatccc attaaatttt tttttgattt atgtaaaagt
     3961 tgcatatacc ttttaaacta catatttggc atttaggtct tataggaata caaatatttt
     4021 gtccatatat tacaaaaata ttatttaaaa ttaaccagta ttttttgtct acaattttta
     4081 ttaattcttc ctctgttttt tttacatctt gtgttttaac aataccaagt ctgttagaaa
     4141 tcctatgaac atgagtatca actggtatgg ccggtttatt aaaagcatat accaaaacgc
     4201 agtttgcagt ttttctccct acaccaggta atgataatag atcttcaagt tcacttggca
     4261 caattccatg aaatttttcg acaatgatct tggatgcttg tttaatcctt tctgatttag
     4321 tattgtaaaa acctatgcta tgtataattg attttacatc gttcaaatct gcattggata
     4381 attctaatgc attcttatat ttctgaaaaa gtttacgtac aatttttgaa gtattctcat
     4441 cccttgaacg agcagataaa atagttccaa ttaatatctt gaagggatca ttatcttctt
     4501 ccatttgaag ttcctttaga gctgtaagtc gtgagaggtt ttcaaagtaa ttcaatgtag
     4561 tttccatttt atcaagaata ttttctattt ttatcaattc ttgagggcga ttattatttt
     4621 gaagttttat acttgatttt ttcttcaatg tttatttatt ttgtagtagg ctaagtctaa
     4681 taaaaaattt aggtatatat gttgatttgt atataatata tgaactacaa attatctact
     4741 gtactaaata tgatcactga tctgggttcc gatatttctg acactctaaa atagcttatc
     4801 tgataaagct ccataatatt gatctttttc aacttatttt tattctgatt ttagtggttg
     4861 attaaatttt atttgcatat tctttatatt tttgtttata tcctaaatct aaattatac
//
LOCUS       DATAFW010000169         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1189936, whole genome shotgun sequence.
ACCESSION   DATAFW010000169 DATAFW010000000
VERSION     DATAFW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189936"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VHJ57_03890"
     CDS             <1..583
                     /locus_tag="VHJ57_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487705.1"
                     /translation="STEAIISEAERIRSLIYNVKVVPRFSDINSIHQTSKFELSELDD
                     NSQNRLDILNKLITEAIKTISREYIQNIKSDDMILLKNEFTKNKKFIVSQNLLGDQSI
                     SSNSHDNYENQLKNFPTLLKIVDRMIEIDENEKNVFIKNIEYNRIDLNGLSFSNKQDK
                     EYRASIVELILEGLRNLDNPIIDRREKGIYENI"
     gene            570..1976
                     /locus_tag="VHJ57_03895"
     CDS             570..1976
                     /locus_tag="VHJ57_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2487706.1"
                     /translation="MKIFSNKSIDFIYFPFTKESDQTLNKKFEDQGKHNISNEILDDL
                     LKSLGREILTEKTPSLQELEQTLQEIIKRKEKNDGINKENDFNDKNDSIVPSDNNGKS
                     NLPIITHLIRKGYLKNSEKWLSSKGFVNIGEKILQDILKELKKGDFGIHETKNFGYGS
                     LLLDTTKKYEIGDDIRLINIPNSILNSIQRLARENGKVEIPIKMDIEDFEEYETIQDV
                     RVSIVYCIDLSSTMRYSTLYGDLSRIEAAKRALWSLYILHKKYFPSDIINMIGFGALA
                     SKINPLDIPYLKTFEPGVDFLHYTNYQAALRLAKKMLQKNPVENKRIVLITDGHPSAC
                     FIDNEKEQQKILSQRPYSHFYIPDQNTINSIKQSHEMNLDINSGALVYLCYRYRQVDQ
                     FIGERTISEAKKCYKKGIQIDTIMISEENSMLSYVNEMEKYVKGRSYYINPDSIGKML
                     LTDYINNKKKVIHSKDNF"
     gene            2025..2834
                     /locus_tag="VHJ57_03900"
     CDS             2025..2834
                     /locus_tag="VHJ57_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX2487707.1"
                     /translation="MSNVVETINYLDWNQSMDILKKAYKINLFALIIGPKGTGKTSLA
                     RKFASEINKNIFSVNFSLRTRESHLVGTKTIENGEINFVEGILVKSMKEGGILYLDEL
                     NAAEPDMLLRLDEALDDRRQIILKEAEGQIIKASEGWFVVATINPLSHVGTKELPPQL
                     LSRFPIRLSLDYPPEETELEIIKQHTMVDNDKLINIQRGIKLARALREAAGVEELFYG
                     PSLRETIAFTKLLNIGTDSKLAAEIVFANAYTQWGEVEYRKVMDMVISIFG"
     gene            2840..>3780
                     /locus_tag="VHJ57_03905"
     CDS             2840..>3780
                     /locus_tag="VHJ57_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX2487708.1"
                     /translation="MTFDNKKKYLRNDILLDIATFLARRWSNNAKVVVTLSKDKSPQT
                     DIEKQQIILPTLNYFHGEEFQRYRQWRVSLWYEAMRIRHTSKVKASSQDHAYGFILNI
                     IETKRIEILGLSGWEGMTNEIIFYESMSWLSKPLLNTIYGRHKIIEAFSQYFLTGYIK
                     GELFGNEFDKVREATDFANECIREALDKRLGTDWIYDKIREIVKILQIDPLLSIPLTT
                     PRSSIGMAMNKLDVFKQLEKIIKSKIVSKEFDKAIKHIIEGKDVVKEYEILVKESKKS
                     ETKGYDSLEDFDISIPNRMDINESEIYDIELIQKIKSA"
BASE COUNT         1575 a          452 c          625 g         1128 t
ORIGIN      
        1 ttccacagaa gctataatta gtgaggctga gcgaataaga tctttaattt ataacgtaaa
       61 ggtagttcct agattttcag atattaatag tatacatcaa acatcgaaat tcgaattatc
      121 tgaattagat gataacagtc agaacagatt agatatttta aataaattga ttacagaagc
      181 aataaagact atctcaagag aatatatcca aaacataaag agcgacgaca tgatactgtt
      241 aaaaaatgaa tttactaaga ataagaaatt tatagtatca caaaatcttt taggagatca
      301 gagcatcagc agtaattccc atgataatta tgaaaatcaa ttgaaaaatt ttcctactct
      361 tttaaaaata gttgatagga tgatagaaat agacgaaaat gaaaaaaatg tttttattaa
      421 aaatatagaa tataacagaa ttgatcttaa tggattatcg ttttctaata aacaagataa
      481 agaatataga gcatcaatag tagagttaat actagaaggg ttaagaaatt tagataatcc
      541 aattattgat agaagagaaa agggaatata tgaaaatatt tagtaacaag tcaatagatt
      601 ttatttactt tccttttaca aaagaatcag atcaaacttt aaataaaaag tttgaagatc
      661 aaggaaaaca taacataagt aatgagatac tcgatgactt actaaaatct ctcggaagag
      721 aaattcttac agagaaaaca ccttctttgc aagaattaga acaaacttta caagagatta
      781 ttaaaagaaa agaaaagaat gatggaatta acaaagaaaa tgattttaat gacaaaaacg
      841 attcaattgt cccatcagac aacaatggaa aatcaaattt acccattatt actcatctta
      901 taagaaaagg ttatttaaaa aattctgaaa aatggttaag cagtaaagga tttgtgaata
      961 ttggcgaaaa aatactacaa gatatattga aagaactaaa aaaaggagat tttggaatac
     1021 atgaaaccaa aaactttgga tatggatctc ttttattaga tacaacaaaa aaatacgaaa
     1081 ttggtgatga tatcagatta attaatattc caaattcaat attaaattca attcaaagac
     1141 tcgcaagaga aaatggtaaa gtcgaaatac cgataaaaat ggatattgaa gattttgaag
     1201 aatatgaaac tattcaagac gttagagttt caatagtata ttgtatagat ctcagttcca
     1261 caatgagata ttcaacattg tatggggatt taagtagaat agaagctgct aagagagcat
     1321 tatggtcttt gtacattcta cataaaaaat attttccttc agacataatt aacatgatag
     1381 gattcggtgc attagcttct aaaataaatc cactagatat cccatattta aaaacttttg
     1441 aaccgggagt ggattttctt cattatacaa actatcaagc ggcattaaga ctagctaaaa
     1501 aaatgttaca aaaaaatcct gtagaaaata aaagaatcgt tcttattact gatggtcatc
     1561 ctagcgcatg ttttattgat aatgaaaaag agcagcaaaa aatactctca caaagaccct
     1621 actctcattt ttatattcct gatcaaaata caataaattc tattaagcaa agtcatgaaa
     1681 tgaatttaga tataaattca ggtgcattag tgtatttgtg ttatagatat agacaagttg
     1741 atcaatttat aggcgaacgt actataagtg aagccaaaaa atgttacaaa aaaggaattc
     1801 agattgatac tataatgata agtgaggaaa actctatgtt gagttatgta aatgaaatgg
     1861 aaaaatacgt aaaaggaaga tcctattata ttaatccaga ttcaataggt aagatgttat
     1921 taactgatta tataaataat aaaaagaagg ttattcattc aaaagataat ttctaatatt
     1981 tatttagtta atttataaat taaactcaag ttgtaatata aacaatgagt aatgtagtag
     2041 aaaccatcaa ttatttagac tggaatcaat ctatggatat attaaaaaaa gcttacaaaa
     2101 taaatctttt tgctctcata attggtccta agggaacagg aaaaacatca ctggctagaa
     2161 aatttgcctc tgaaattaat aagaatattt tttcagtaaa ctttagtctt cggacaagag
     2221 aatcacatct agtgggaact aaaactatag agaatggtga aattaacttt gtagagggaa
     2281 ttttggtaaa gtcaatgaaa gaaggaggaa ttctttattt agatgaatta aatgctgcag
     2341 aaccagatat gctcttaaga ttagatgagg ctttagatga tagaagacaa ataatattga
     2401 aagaagcaga ggggcagatt attaaagcta gtgaaggatg gttcgtagtt gctacaatta
     2461 atccgttatc gcacgttggt actaaagaat taccacctca acttttaagc aggttcccaa
     2521 ttagactaag tttagattat ccaccagagg aaaccgaact cgaaattatt aaacaacata
     2581 ctatggtaga taatgacaag ttaattaata ttcaacgtgg tataaaatta gcaagagcat
     2641 tacgagaagc agctggagta gaggagctat tttatgggcc tagtttaaga gaaacaattg
     2701 catttactaa attactcaat attggtaccg actctaagct tgcagctgaa atagtttttg
     2761 caaatgccta tactcaatgg ggagaagtag agtatagaaa agttatggat atggttattt
     2821 cgatattcgg ttgataactg tgacatttga taataaaaaa aaatatttaa gaaacgatat
     2881 tctgctagac atagctacat ttcttgcacg tcgatggtct aataatgcaa aagtggttgt
     2941 tacactttct aaagataaat ctcctcaaac agatattgaa aaacaacaga ttattttacc
     3001 aacattaaat tattttcatg gtgaagaatt tcaacgatat agacaatgga gagtttcttt
     3061 atggtatgaa gcaatgagaa taagacatac ttctaaagta aaagcctcta gtcaggacca
     3121 tgcatacggg tttattctta atattataga aacaaaaaga atcgaaatat taggtttaag
     3181 tggctgggaa ggtatgacaa atgaaataat tttttatgag agtatgtctt ggttgtcaaa
     3241 acctctttta aatacaatat atggcagaca taaaataata gaagcttttt cacaatattt
     3301 tcttactggt tatatcaaag gagaactatt tggaaatgaa tttgacaagg taagagaagc
     3361 aacagacttt gcaaatgagt gtatcagaga agctcttgat aaaagattag gaacagactg
     3421 gatttatgac aagataaggg aaattgtcaa aatattacaa atagatcctt tattatcaat
     3481 tcctttgact actcctcggt ctagtatagg tatggcaatg aacaaattag atgtatttaa
     3541 gcaactagag aaaataataa aatcaaaaat agttagtaaa gaatttgaca aagcaataaa
     3601 acatattatc gaaggaaaag atgtcgtaaa agaatatgaa attttagtaa aagagagtaa
     3661 gaaatcagaa acaaaaggat atgacagttt agaagacttt gacataagta ttcctaatag
     3721 aatggatatt aatgaatcag aaatctatga catagaatta attcaaaaaa ttaaatcagc
//
LOCUS       DATAFW010000170         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6309774, whole genome shotgun sequence.
ACCESSION   DATAFW010000170 DATAFW010000000
VERSION     DATAFW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6309774"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VHJ57_03910"
     CDS             complement(<1..195)
                     /locus_tag="VHJ57_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX2487709.1"
                     /translation="MITIDNLLMQMPFGFEWIFIIVIIVVLFFGVKKIPELAKSFGKA
                     TAEFEKAKIEAKRDLEKYKKE"
     gene            350..667
                     /locus_tag="VHJ57_03915"
     CDS             350..667
                     /locus_tag="VHJ57_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487710.1"
                     /translation="MQYHKAAFLGQERSRKAQMKLFEYTGFSMLTYTIKKGSSGFEPV
                     GEEMLVGSLNNGKEMILFICDNEGYAKAQSKSMTIEDGNNIVEKMILDGFKKFEGEIK
                     TVI"
     gene            complement(747..1466)
                     /gene="pyrH"
                     /locus_tag="VHJ57_03920"
     CDS             complement(747..1466)
                     /gene="pyrH"
                     /locus_tag="VHJ57_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX2487711.1"
                     /translation="MVEYRQKKRIVIKLSGSIFSLSDNNDQDSNNYYNIFKQYSDILA
                     TLTSNVQLIVITGGGSIARLYINFARKLGLDEASLDLLGIAISRVNAKLLIASLGHYA
                     YPEVPISLDDVGRFVESNKIIVSGGLHPGQSTNATSALIAEKTRATEFINATDVKGIY
                     DSDPRKNKNAQLFKKIEVGKLLNIILKESTMAGEYDLLDIVALKVIERSKIRTKVILS
                     DPKNISNTVLGKKYIGTELVL"
     gene            complement(1478..2719)
                     /locus_tag="VHJ57_03925"
     CDS             complement(1478..2719)
                     /locus_tag="VHJ57_03925"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX2487712.1"
                     /translation="MVLEFTGEITDPIHKYIRITDLEKKLIDTEVFQRLRKIRQLAGA
                     HLVYPSAQHTRFEHSLGAMHLAGLAAETLLDKGYITYKEDVESLRIAALLHDIGHGPF
                     SHLFDEVLSENESKKINHEIIGKKIIKETIIIDILRKYGYDGDYVCKLSFGESKKMFY
                     NEIIAGSLSADIMDYLPRDSFFTGAEYGKVDYYRLISSFEVLSNGHLGINKSAINSLE
                     SMLISRYQMFKAVYFHKTVRAGEVMLLHSLMSADKQLNFSNFSIEEYLDYTDEKTIDI
                     ICSLTGNKFASDLALDYKKRKLFKCVYEKFLQNKYNAFDKELAGKQIREFLEGVTESL
                     DIDERSIFIDISRAPSMPLTPSKEEFNSIMLVDKNLEFETPTSDIPLINSITGFLDMI
                     RIYTPERNREKLEKVFKNIDL"
     gene            complement(3067..3387)
                     /locus_tag="VHJ57_03930"
     CDS             complement(3067..3387)
                     /locus_tag="VHJ57_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487713.1"
                     /translation="MSIQEFLLPDENIRFHSKTSVKYGEKKYNVLITDVRIILYAQRG
                     HILKTDDVISERLDKLREIKYCEKGLVFRSAKISIQSSINLDIVGSCTELKQLFNNLQ
                     VIVY"
BASE COUNT         1295 a          575 c          465 g         1373 t
ORIGIN      
        1 ttctttttta tatttctcta gatctcgttt agcttcgatc ttagcttttt caaactctgc
       61 tgtagccttc ccaaaagatt ttgctaattc agggattttt tttactccga agaataatac
      121 aactataatc actataataa atatccattc aaaaccaaaa ggcatctgca tcaatagatt
      181 atctatggta atcaaacttc tttctaatat tgttagtatc aataataatt taagtgttaa
      241 attaatatag ttacattttt ttcaatagat aggtatttgt tgaaaagtct tatttttaat
      301 aaaactagat agacattaat aattatcagt caatactaaa ttatctctaa tgcaatatca
      361 taaagctgca ttcctcggac aagaaaggtc aagaaaagct caaatgaaat tgtttgagta
      421 tacaggattc tccatgttaa catacacaat aaaaaaagga agttctggct ttgaaccagt
      481 aggagaagaa atgttagttg gaagtttgaa caatggcaag gaaatgattt tatttatctg
      541 tgataatgag ggctatgcaa aagcccaatc aaaatccatg acaattgaag acggtaataa
      601 catagtagaa aaaatgattc ttgatggttt taagaaattt gagggagaaa tcaaaacagt
      661 gatttgataa tgacagtatt atgacattat ttatacgata agattctaaa ttgggtgcat
      721 ttatcatgat tattattcat ttttagtcac aatactaatt cggttccaat atactttttc
      781 cctagtacgg tattagatat atttttggga tcggaaagaa ttacttttgt tctaatttta
      841 gatctttcta ttacttttaa agctactata tccagtaaat catattcacc agccattgta
      901 ctttctttta atattatatt tagtagctta cctacttcaa tttttttaaa taattgtgca
      961 tttttatttt tcctaggatc agaatcataa attcctttga cgtcagttgc attgataaat
     1021 tcagttgctc ttgtcttttc tgcaattaga gcggaggtag cattagtact ctggcccgga
     1081 tgtaatcctc ctgaaacaat tattttatta ctttcaacaa acctacctac atcatctaat
     1141 gaaataggaa cttcaggata agcataatga cccaaagaag caataagtaa ttttgcattt
     1201 actcgtgata tagctatacc gagcaaatct aggcttgctt catccagtcc taattttctt
     1261 gcgaaattga tataaagtct tgctatactt ccacctcccg taataactat taattgtaca
     1321 ttagatgtga gagtagctaa aatatcacta tactgtttaa aaatattgta atagttatta
     1381 ctgtcttggt cattgttgtc agatagacta aaaatacttc cactcaattt tattactatt
     1441 cgtttttttt ggcgatactc aaccaattct attcccttta aagatcaata ttcttaaaca
     1501 ctttttccaa tttttctcta tttcgttcag gcgtataaat acggatcata tctaaaaaac
     1561 ctgttattga attaatcaat ggaatatcgg aagtaggcgt ttcgaattct aaatttttat
     1621 caactaacat tattgaattg aattcttcct tggaaggagt aaggggcatt gaaggagctc
     1681 gcgaaatatc aataaatatg gagcgttcat caatatccaa agattcagtt accccttcca
     1741 aaaattctct aatttgttta cctgctaact ctttatcaaa tgcattatat ttgttttgta
     1801 aaaatttttc ataaacacat ttgaaaagtt ttcttttttt ataatccaaa gccaaatctg
     1861 aagcaaattt atttccagtt aaagagcaaa taatatctat tgttttttca tcagtatagt
     1921 ccaaatattc ttcaatagaa aaatttgaaa aatttagctg tttatctgca ctcataagag
     1981 aatgcaataa cattacttca cctgctctca cagttttatg aaaatataca gctttaaaca
     2041 tttgatatct agaaattaac atagattcaa gagaattaat tgcagattta ttgataccta
     2101 agtgaccgtt tgaaagaacc tcaaaggaac taattaacct ataataatca acttttccat
     2161 attctgcacc agtaaagaaa ctatctcgag gcaaataatc catgatatct gcggataaac
     2221 ttcctgctat gatttcatta taaaacattt ttttggattc tccaaaagaa agcttacaga
     2281 cataatcacc atcataccca tactttctca aaatatcaat tattattgtt tcttttatta
     2341 ttttttttcc tattatctca tgattaattt tctttgactc attctcactg agaacttcgt
     2401 caaaaaggtg agaaaaaggc ccatgtccga tatcatgtaa taacgcagct atccgtaaac
     2461 tttccacatc ttctttataa gtaatatacc ctttatctag aagagtctct gctgctagtc
     2521 ctgctaaatg catggctcct aaggaatgtt caaaacgggt atgctgagca ctaggatata
     2581 ctaagtgagc ccctgccagt tgccttattt ttcttaatcg ttgaaaaact tcagtatcaa
     2641 ttaatttttt ttctaaatct gtaatacgga tatacttatg aataggatca gttatctctc
     2701 ctgtaaattc taaaaccatt taagtagatt attataaaaa tatattttct tataaacggt
     2761 gttgatatgg caaacagata aacctatttt ttgaataatc aaaaataaaa tgatgtttaa
     2821 ttttatattt atctaatccc tataaaagag aatcattatt catctatcac tctatatatt
     2881 aaacaaatag taataataaa gatatctaaa atggtaaaag tcaaatgtta aatacaggta
     2941 tttgatatta aaatgaccca aatattataa ttgatacaag tattaattat aatattttaa
     3001 tagtagaaat ttattttccc ttttcaggat acaaattaag ataattaaat aaatttatac
     3061 tagatatcaa taaacaataa cctgtaaatt attaaataat tgttttaatt ctgtgcaaga
     3121 tccaactata tcaagattta tacttgattg aattgaaatt tttgcacttc taaatactaa
     3181 tcccttttca caatatttta tttcacgtaa tttatctaac cgttcgctaa taacatcatc
     3241 ggtttttaaa atatgtcccc tttgtgcata aagtattatc ctaacatcag ttatcaaaac
     3301 gttatatttt ttttcaccat acttcacact tgttttgcta tggaatctaa tattttcatc
     3361 agggagtaga aattcctgta tcgacatatt tgttatacta aaacgatacg attagtatta
     3421 tttatcaact agagtcataa ctaattatcc aaattgataa tttaattaaa tacgaaattt
     3481 acaaaaaaaa ttatcaaata acatcaacaa tgttatcttt tttattaata tatattatta
     3541 ttcatcttgt ttaaatagat ataaaatata tttcactatc gagatcttta aacatatgcc
     3601 cagtgagatg tctttaagag aacatttttc tgaaatgagg tccaggatat tgagggtttc
     3661 tattattatc ttaataatta ttgtcttttg tattacattt gggttcaa
//
LOCUS       DATAFW010000171         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_668687, whole genome shotgun sequence.
ACCESSION   DATAFW010000171 DATAFW010000000
VERSION     DATAFW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668687"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..726)
                     /gene="pdxT"
                     /locus_tag="VHJ57_03935"
     CDS             complement(100..726)
                     /gene="pdxT"
                     /locus_tag="VHJ57_03935"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX2487714.1"
                     /translation="MSNIKVGVLGIQGDIEENVLATAEALKKLNLRGTVESVRYSESI
                     ADLDGLILPGGESTVIGSLLSIQGKVFQTIEKKITEGMPVLGTCAGLIMLSKRTYDKV
                     VGDTKQKLFGMLDVVIERNAFGRQHESFEADLEIPVLGKEPFRGIFIRSPIVNNVGES
                     VTILTKLNGKIVAVKQNNIIGTSFHPELSSDRRLHELFIKDIVGFRKD"
     gene            complement(723..1694)
                     /gene="pdxS"
                     /locus_tag="VHJ57_03940"
     CDS             complement(723..1694)
                     /gene="pdxS"
                     /locus_tag="VHJ57_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX2487715.1"
                     /translation="MTISIVATEEERNGIKFKNLHNEQLVQGTTLVKRGFAHMQKHGV
                     IMDVTTVEQAQIAEDAGAVAVMVLDKLPYDVRKAGGVARTASIKVIEEIMNEITIPLM
                     AKCRIGHISEAKILEVIGVDMIDESEVLTPADEENHIWKWDYTTPFVNGAKNLGEALR
                     RIEEGSSMVRTKGEPGTGNIAEAIRHIKQVNNDIRKLRSFYFDKDDQELIKFSRELKI
                     SYPLIKETAKLARLPVVNFAAGGISTPADAALVMNLGCDGVFVGSGIFKAEDPVQRAK
                     SIVLATTYYDDPTIVMEAQKLVDEKKSLLGLDTKSLELRMQERGPMI"
     gene            complement(1797..2423)
                     /locus_tag="VHJ57_03945"
     CDS             complement(1797..2423)
                     /locus_tag="VHJ57_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487716.1"
                     /translation="MSNNLGDNSQTSFYLNGSIFSLIFIGDEVHVKDNNDFANLLNLD
                     KEKEDVNFVDFLTNSLIDIISNEGDSNFSLEQVLSNSNITTSNNPNNLKKLLLLNGSW
                     NLDVKNGNIVSFDAKISTINKDGMSPHMYEILNLVVVNGNTPIKLSQNDSTIIEGRAD
                     IITPDNIKNIGLYLILQENSISIILDPKILGNDYNGQPIYGLTNFMKN"
     gene            complement(2594..2953)
                     /locus_tag="VHJ57_03950"
     CDS             complement(2594..2953)
                     /locus_tag="VHJ57_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487717.1"
                     /translation="MISEISEYTKICNAICSVIADLKEEGIDINTKFDKEYGFIGIYT
                     SNTSILKKAKNGLNEILELSYSTAEHHPYWNIIYNTAEIARVILDTWNENFSLDQIQE
                     MLWRNDEIKNTLNRLEK"
     gene            3255..3410
                     /locus_tag="VHJ57_03955"
     CDS             3255..3410
                     /locus_tag="VHJ57_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487718.1"
                     /translation="MNTTAKKPIGDNEPPCDYCGNPIEICMCVCPYCGETNSCECCLY
                     DAVTGGG"
     gene            3459..>3767
                     /locus_tag="VHJ57_03960"
     CDS             3459..>3767
                     /locus_tag="VHJ57_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015020309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEX2487719.1"
                     /translation="MKDKIVNEITWLIGGAQGSGVDSAANIFSKTCAEDGLYIFGKRE
                     YYSNIKGEHSYFTVRVSNKPIRSHKESIDILVSFDAETLFRHAKDVSTDGIIIFNSEL
                     I"
BASE COUNT         1249 a          618 c          490 g         1410 t
ORIGIN      
        1 aaacaatttg gagacgcggg acttaagatc taaaaaatgg gtgatcccgt cctttagagg
       61 ttcgtgggtt caaatcccac cccccgcatg ttatataaat taatcttttc taaatcctac
      121 aatatctttg ataaataatt catgtaatct tctatctgat gaaagttccg gatgaaatga
      181 tgttccaata atattatttt gttttacagc aacaatttta ccattaagtt tagttaggat
      241 agttacgctc tctcctacat tatttactat aggcgatctt atgaaaattc ctctaaaagg
      301 ttccttacct aaaactggaa tttcaagatc tgcttcaaag gattcatgtt gtcttccgaa
      361 tgcatttcgt tcaattacaa catctaacat tccaaataat ttttgttttg tatctccaac
      421 aactttatca tatgttcgtt ttgataacat tatcaaaccc gcacacgtac ctaatacagg
      481 cataccttca gtgattttct tctctatagt ttgaaagact ttaccttgaa tagagagcaa
      541 acttccaatt acagtacttt caccacctgg taatattaaa ccatctaaat ctgctatgga
      601 ttcagaatag cgaactgact ccacggttcc tcgtaagttt agcttcttta atgcctcagc
      661 tgttgctaat acattttctt ctatatctcc ttgaattcct agtactccta ccttaatatt
      721 gctcaaatca tagggcctct ttcctgcata cgtaattcaa gacttttagt atctagacct
      781 aatagcgact ttttctcatc tactaatttt tgagcttcca taactatcgt aggatcatca
      841 taatatgttg tagctaagac tattgattta gctctttgta ctggatcttc agctttaaaa
      901 ataccagagc caacgaaaac accatcacat cctaaattca taactaatgc tgcatcagca
      961 ggtgttgata taccacctgc cgcaaaattt actactggta atctagctag tttagcagtt
     1021 tccttaatta atggataaga aattttaagt tctcgggaga atttaattaa ttcttggtcg
     1081 tctttatcaa aatagaaaga tctaagtttt ctgatatcat tatttacttg tttaatatgt
     1141 cgtatagctt ctgcaatatt tccagttcct ggctcacctt tagttctaac catagaactt
     1201 ccttcttcta ttcttcgtag tgcctcgcct aaattctttg caccatttac aaaaggagta
     1261 gtatagtccc atttccatat atgattctcc tcatctgcag gtgtaaggac ttcactctca
     1321 tcaatcatat caacacctat cacttccagt atttttgctt ctgatatatg cccaatccta
     1381 catttagcca taagtggtat tgtaatttca ttcattattt cttcaataac tttgatacta
     1441 gcagtcctag cgactcctcc agcctttcta acatcataag gtaatttatc taaaaccatg
     1501 actgcgacag caccagcatc ttctgcgatt tgtgcctgtt ctacagtagt aacatccata
     1561 ataacaccgt gtttttgcat atgagcaaaa cctcgcttta ctaatgtagt tccttgaact
     1621 aattgttcat tatgtaaatt cttaaatttg atgccgttac gctcttcctc tgttgctaca
     1681 atagaaattg tcattattta aatttattaa aaagcatcta ttataggttt agtttagtat
     1741 gacgtaatat gtaaagttaa acagtaacca tattggacaa ataattgtgt aaacatctaa
     1801 tttttcataa aattggttaa tccataaata ggttgtccat tgtaatcatt tcctaatatt
     1861 ttggggtcta aaataatact tattgaattt tcttgcaaaa ttaaataaag accaatgttt
     1921 ttaatattat caggagttat aatgtctgct ctaccttcta taatagtact atcattttga
     1981 gataatttaa taggagtatt accatttaca actactagat ttagaatttc atacatatgt
     2041 ggagacattc catctttatt tattgtagaa attttagcat caaatgatac aatattccca
     2101 ttttttacat ctaagttcca actaccatta agaagaagaa gtttttttaa attattagga
     2161 ttattagatg tagtaatatt tgaattggaa agtacttgtt ctaaagaaaa atttgaatca
     2221 ccttcgttag agataatatc aattaaacta tttgttaaaa aatctacaaa atttacatct
     2281 tctttttctt tgtctaaatt taaaagattt gcaaaatcgt tattatcctt cacatgaact
     2341 tcatctccaa tgaagattaa agaaaatata gaaccattta gataaaagga tgtttgagaa
     2401 ttatctccta aattattaga caaatttaaa attggaatag actctccaaa atatagaaat
     2461 ttgaatatta ctaaaaaaac taaagtaatt acaaataaaa ttataagcca tttcattaaa
     2521 taactcttta tattttttaa tattattaaa tttatatgat tattgctacc aataccaaac
     2581 taaaagataa atcttatttt tctagtctat ttagagtatt tttaatttcg tcatttctcc
     2641 aaagcatttc ttgtatctgg tcaagactaa aattttcatt ccaagtatct agtatgactc
     2701 ttgctatttc agcagtatta tatattatat tccaataagg atgatgctct gctgtagagt
     2761 atgataactc taatatttcg tttaaaccat ttttagcttt ctttaaaatc gaggtattag
     2821 atgtatatat tccaataaat ccatattcct tatcaaattt agtgtttata tctattcctt
     2881 cttcttttag atctgctata acagaacaaa tagcattgca aattttagta tattctgaaa
     2941 tttcagaaat catattttat atatccttgt gttactttat ttatagatta gaaataatgg
     3001 aaaatagcta tgatattcaa aatttttata tcaaattaaa tagtatctta ttagattatt
     3061 cctcagccaa attatctatg ctttcaaatt catcataact ttctttcaag tcctcttcaa
     3121 attcagtttc atgttcttcg ttagacataa tatttacttg ttataattca aaaataaatc
     3181 ttttcataat tataataatg aaattaaatt cttataaata attatttata agaaggtata
     3241 taattatatt aaaaatgaat accacagcca aaaagcctat tggggataat gaacctccat
     3301 gtgattattg tggaaatccc atagaaatat gtatgtgtgt atgtccgtat tgtggcgaaa
     3361 ctaattcttg tgaatgttgt ttatatgacg ctgttactgg tggtggataa agtattttta
     3421 agatatttat tagataaaag caccttgtaa attatagaat gaaagataaa attgtaaatg
     3481 agatcacttg gttgattggt ggagctcaag gaagtggtgt tgattctgct gctaatatat
     3541 tttctaaaac ttgtgcagaa gatggattat atatttttgg aaaaagagaa tactattcaa
     3601 atataaaagg cgagcatagc tatttcactg ttagagtatc aaataaacca atacgatcac
     3661 ataaagaatc tatagatatt ctagtatctt ttgatgccga gactttattt agacatgcaa
     3721 aagatgtgtc tactgatgga atcattatat ttaattctga attgatt
//
LOCUS       DATAFW010000172         5044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_1729732, whole genome shotgun sequence.
ACCESSION   DATAFW010000172 DATAFW010000000
VERSION     DATAFW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729732"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1344
                     /gene="aroC"
                     /locus_tag="VHJ57_03965"
     CDS             241..1344
                     /gene="aroC"
                     /locus_tag="VHJ57_03965"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX2487720.1"
                     /translation="MGGNSIGDQFVVTSFGESHGRCIGSVLDGCPAGLPLKESDIQPE
                     LDLRKPGQSTITTQRKEEDKVEILSGTFNGYTTNAPICMVIWNADSDSRPYEKIKTIP
                     RPGHSDYPAMVKYGGYADYRGSGRFSGRLTASFVMAGAIGKKLLKYVLDIETIAYTAE
                     IGDISIGNISFANAKEYRYTNEVRCPDLSIAEKMKDSIMSARKSGNSLGGIVECVSMG
                     LPVGLGEPIFSSLESDLSKALFSIPAVKAIEFGSGFKGTKRTGYENNDLFIKDEGRVV
                     TLTNNCGGILGGLSNGMPLTIRIGFKPAASIAKTQKTLDLSSNSLVDLQVPGRHDPCV
                     VPRAPPIVEGIVSIVLSDHAIKAGFIPTVLKGY"
     gene            1346..2722
                     /locus_tag="VHJ57_03970"
     CDS             1346..2722
                     /locus_tag="VHJ57_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX2487721.1"
                     /translation="MVEQQNLDYLREEMQNITNQIIKLVHDRLEVAKKIGNIKQQLKI
                     EIEDEKVENDIKKTVLKLTKDIGMDVEFSGRLLNLLLSESVKLQSVQNTSNKSSTHMA
                     IFMKAKELEKTGKKIIHMEVGEPDYPPPLKIKEELAKIYDNKRYHYTEISGIPQLRQA
                     ISQKFSNILPEQVLVTPGGRFAVFCTISSLLKSGDELINIEPSWPAYKECADFVGAKT
                     RVLKTTLEDKWDPNLKVLENLININTKMIIINYPNNPTGKILDSHLMEKIVSIARDYN
                     LYILSDEVYSDYSFGKYNSILDYQYDKSIMVSSFSKTYGMTGFRVGYGIANNSIITQM
                     RKVQATSITSVAEPMQYSALVALGNNVQSNIDSIQSRINLICKRLQEMKTEFLIPNGA
                     MYVFPRILAKSYSDDISLVYDLLKNGVAIAPGSGFGNSYKKFIRISACQPSNLLNDGL
                     DILAKYLK"
     gene            2737..3609
                     /locus_tag="VHJ57_03975"
     CDS             2737..3609
                     /locus_tag="VHJ57_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX2487722.1"
                     /translation="MKEIAIIGAGGRMGSWFTKYFVNNKNFRIKVYDKNMYSIAKQLK
                     VKIEIDFNKCIKDSDIVILCIPLRMIPQMIHKCSRIMKRNSVLIDISSIKSKSFVSLS
                     KIQNSISPICIHPMFGPGASQTTRLKILFIPVKNYNKELQFVRQLFQNFEILPIENAI
                     KHDHIMAVVLGLNHYMNIIFADIISSHKYKDLEFYSGNTFRIQCILSESILNDDIDLL
                     NSLFIENPFIKKEIKNFHKKFLKYYQMVNDKDDEKLEKKLKNVKSSIEEYHDLNSSYV
                     KMYKFIDLLCKNKN"
     gene            complement(4495..5016)
                     /gene="cgi121"
                     /locus_tag="VHJ57_03980"
     CDS             complement(4495..5016)
                     /gene="cgi121"
                     /locus_tag="VHJ57_03980"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HEX2487723.1"
                     /translation="MVLFKLKNKYVYFHTVFFTQYHDFTIIDKLRKEFPDTFIQALNF
                     NIIYNYEHLFEILGISDLAKTRNIMCSHKIELDFLLRVMGTNQISQAIKEGGITIDQY
                     ITFVVLGKKYLIDKIHQKLEFLFGNSHDEVFTEKSILRKKIEAYKTKYNNDFFDEENT
                     IKFLTEAAVLVMK"
BASE COUNT         1862 a          711 c          782 g         1689 t
ORIGIN      
        1 acaatgatca tagacttttt atggcattta caattgcatc tatgttgacg aaaaaatcaa
       61 tggtaagagg agccgaatct atagatgtat cttatccttc cttcattcac gaaataaaaa
      121 gacttggcgc taatgtaaat ttaattaact aaatagatga caatcaaatt acaaaatcaa
      181 tatcgcaatt cttaaaaagt cactattata taggactagt tatcctttgg gtatgatatt
      241 atgggtggaa atagtatagg agatcaattc gtagtcacta gttttggaga aagtcatggt
      301 cgatgtatag ggtctgtttt agatggttgt ccagcaggcc ttccattgaa agaatctgac
      361 attcagccag agttagattt acgaaagcct ggacaatcta ctattacgac tcaaagaaaa
      421 gaagaggaca aggtggagat tctttcaggt acatttaatg gttatacaac taatgcacct
      481 atatgtatgg tgatttggaa tgcagattca gattcaagac cgtatgaaaa gataaaaaca
      541 atcccaagac caggacactc tgattatcct gcgatggtaa aatatggggg atatgcagat
      601 tatagaggaa gtggtagatt ttctggtaga ttaacagcaa gtttcgtaat ggcaggagca
      661 atcggtaaaa aattattgaa atatgttctc gatatagaaa caattgctta tactgcagaa
      721 ataggagaca tctctattgg caatatttct tttgcgaatg caaaggagta ccgttacact
      781 aatgaagtca gatgtcctga cctctctata gccgaaaaga tgaaagattc aattatgagt
      841 gcgagaaagt caggaaattc tttaggcgga atagtagaat gtgttagtat gggattaccg
      901 gtgggactag gagaacctat tttttcatca ttggaatcgg atttaagcaa agcattattt
      961 agtattcctg ctgttaaagc aatagaattt ggttctggtt ttaaaggaac aaaaaggaca
     1021 ggttatgaaa ataatgattt atttataaaa gatgaaggaa gagtggtaac attaacaaat
     1081 aattgtggtg gtatcttagg cgggttatct aatggaatgc cacttactat aagaatagga
     1141 tttaaacctg cagcctctat agcaaaaact cagaaaacat tagatttatc ttcaaactct
     1201 ttagtagatc ttcaagttcc aggaagacat gatccttgtg ttgttccacg cgctcctcct
     1261 attgttgaag gaattgtctc catagttttg tcagatcacg caattaaagc tggattcatt
     1321 cccacagttc ttaagggtta ttgaaatggt tgaacagcag aacttggatt atttaagaga
     1381 ggaaatgcaa aatattacta atcaaataat taaattggtt catgatagat tggaagtagc
     1441 aaaaaaaatt ggtaatatta aacaacaatt gaaaattgag atagaagacg aaaaggtaga
     1501 aaatgatata aaaaaaaccg tcctcaaact tacaaaggat ataggcatgg atgtagaatt
     1561 ttctggcaga cttttgaatc ttcttctttc ggaatctgta aaattacaaa gtgttcaaaa
     1621 tactagtaac aaaagctcta ctcatatggc tatatttatg aaggctaaag aacttgaaaa
     1681 aactggaaag aaaataatcc atatggaagt tggtgagcct gattatcctc ccccattaaa
     1741 aattaaagaa gaattagcaa aaatatatga caataaaaga tatcattata cggaaatttc
     1801 tggaatacca caactccgcc aagccatatc tcaaaaattt tcgaatattt taccagaaca
     1861 agttctagtt actccaggag gcaggtttgc ggttttctgt acaattagct cgcttttaaa
     1921 atcaggggat gaattaatta atatcgaacc atcgtggccg gcttataaag aatgcgcaga
     1981 ctttgttggt gctaaaacca gagtactaaa aactactctc gaagataaat gggacccgaa
     2041 tttaaaagtg ctagaaaacc taataaatat taatactaaa atgattataa taaactatcc
     2101 taacaatccc acaggaaaaa tacttgattc tcatttaatg gagaaaatag tttccatagc
     2161 aagagattat aatttatata ttttaagcga tgaggtttat tcagactatt catttggtaa
     2221 atataatagc attttagact atcaatatga taaaagtata atggtatcct ctttttcaaa
     2281 gacttatgga atgacaggat tcagagtagg ctatggaata gcaaataaca gtataataac
     2341 acagatgcgt aaggtccagg ctacatctat tacatcggta gcagaaccaa tgcagtattc
     2401 tgcgttagtc gcactaggaa ataatgttca atcaaatatt gattctatcc aatcacgaat
     2461 caatctgatt tgcaaaaggt tacaagaaat gaaaacagaa ttccttattc ccaacggtgc
     2521 aatgtatgtc tttccaagga ttttagctaa aagttactca gatgatattt ccttagttta
     2581 tgatttattg aaaaatggag tagcaatagc gccaggaagt ggatttggaa attcttataa
     2641 aaaatttatt agaatttctg catgtcaacc atcaaacttg ttaaatgacg gtttggacat
     2701 tttggcaaaa taccttaaat aggtaatcaa cctaagatga aagaaattgc tataattggg
     2761 gcaggaggtc gaatgggatc ttggttcaca aagtattttg taaataataa aaactttcgt
     2821 atcaaagttt atgataaaaa tatgtactca atagcgaaac aactaaaggt aaaaatagaa
     2881 attgatttta ataagtgtat taaagattct gatatagtaa ttttgtgtat tccattgaga
     2941 atgataccac aaatgataca taaatgttct cgaataatga aacgaaattc agttttaatt
     3001 gatatctctt ccattaaaag taaatccttt gtatctctct ctaaaattca aaattctatt
     3061 tcgccaattt gtattcaccc aatgtttggg ccaggggcat ctcagaccac tcgcttaaag
     3121 attttattca ttccagtgaa aaactataat aaagagctcc agtttgtaag gcaactgttt
     3181 cagaattttg aaattttacc aattgaaaat gcaattaagc atgaccatat catggctgta
     3241 gttttaggtt tgaatcatta tatgaatata atattcgcag atattatttc atcacataaa
     3301 tataaggatt tagaattcta ttccggaaat acatttcgaa tacaatgtat tttatccgaa
     3361 agtattttaa acgacgatat agacttacta aattctttat tcatagaaaa tccatttatt
     3421 aaaaaagaaa taaaaaactt tcataaaaaa tttttaaaat attatcaaat ggttaatgat
     3481 aaggatgacg aaaaattaga aaaaaaatta aaaaatgtaa aatcgtccat tgaagaatac
     3541 catgatctta attcttccta tgtaaaaatg tataaattta ttgacttact ctgtaaaaat
     3601 aaaaattaaa tacatgattt agactgcatc gtaatctttg tgacttctct ttaacacttc
     3661 taagattata ccagaaacat tcaatattct agtcatagaa tttaagcgag caattatttc
     3721 tatttctcaa tctcaatact acattaatta tacattttct ggattcattt tcctaatatt
     3781 aatttcttct ttcattttct taatttgatt aaccaaattc ttcattatta tgcatatttt
     3841 tttatatttt tttatacgat catgaactta aatgatagca gaacttaatc ttttgtgtct
     3901 tctaaattaa aatcatgata aaaacataat aaagttcggc actacaatac tttaaatatt
     3961 gagtagtcat catcttaatt gattttttat cggtatgttg tgtccataca aaaaattctc
     4021 gtcttgtacc taaagatgat aattcgaagt atttaaataa taaaagatta taaagaaaaa
     4081 atcgaagaat ttaaagattt tgaaatttat tgaagcagat ggtaatacaa acagaacttc
     4141 taagacttaa cctagtatta cttaagttca gtttcgtcat ttcaaaaaaa tgatttttat
     4201 tcgactatca aataatagag atctatctat gaaattgata tgaaggataa tcaaaattta
     4261 tatgaaataa ggactttaaa tatatgtatc cttatacttt caatcattct atttcaatag
     4321 ttatttttat gaaatctatt atattcttaa aaaaatggac ttactcggat acttcaattt
     4381 ttttattttt ttatcgcagt catatattca caactaaaat cctcaggttt tagatgtcaa
     4441 ttttcgataa tctttttaca aggtttttta aaaaggtata aatctaatca aaatctattt
     4501 cataactagg actgctgctt cagttagaaa tttaatagta ttttcttcat caaaaaagtc
     4561 attattatat tttgttttat atgcttcaat tttttttctt aaaatggact tctcagtgaa
     4621 aacttcgtca tgagaattac caaataaaaa ctctagtttt tgatgaatct tatctatcag
     4681 gtactttttt cctaatacaa caaaagtaat atactgatca atggttattc caccctcttt
     4741 aattgcttga gaaatttgat tagttcccat gactcttaac aaaaaatcta attctatctt
     4801 atgggaacac attatattcc ttgtttttgc taaatctgat attcctagaa tctcaaaaag
     4861 atgttcgtag ttataaatta tattaaaatt taatgcttgg ataaaagtat ctggaaactc
     4921 ctttcttaat ttatcaataa ttgtaaaatc atgatattga gtgaaaaata cagtatgaaa
     4981 gtagacatac ttatttttta gtttgaaaag taccaaatta atttattcta atttatctat
     5041 ccac
//
LOCUS       DATAFW010000173         1595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5438069, whole genome shotgun sequence.
ACCESSION   DATAFW010000173 DATAFW010000000
VERSION     DATAFW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5438069"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VHJ57_03985"
     CDS             complement(<1..1315)
                     /locus_tag="VHJ57_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX2487724.1"
                     /translation="MMKASDLFLRCLESEGVEYIFGLPGEENNDLMFSLLNSKIKFIL
                     VRHEQGAAFMADVYGRLTQKVGVCLSTLGPGATNLITGVANANMDRSPILAITGQTDS
                     NLRHKESHQNMDVVSMFYPITKWRWSIHNTDVIAELVRRAFKISIEEKAGAVHLELPE
                     DIAKEKSDIKPIKKPKRTLRSRPQKGLIKKAVKMILSAKIPIILVGNGCIRGSASSNI
                     RRFVEKTGIFSMNTFMAKGVISDKSERHLQTIGIQNTDNLLIALRESDLVIAIGYDLV
                     EYSPKNWNRNLDKKIIHIDFTSAEVETYYPPDIEIAADIGYTIDAILHELIKEKEVEE
                     EKKEKEGKKINKNDNLNSIDIPYFEIPDTLREIKKQITESITNFKDDFAYPINPMKLI
                     IDVRNALEENDIVISDVGAHKLWIAKTYNTYIPNTCLITNGFCSMG"
BASE COUNT          491 a          262 c          204 g          638 t
ORIGIN      
        1 aacccataga acaaaaacca ttagtgatta gacaagtatt tggaatataa gtattgtaag
       61 tttttgctat ccataattta tgagctccaa cgtctgatat aacaatatca ttttcttcta
      121 aagcatttct tacatcaatg attaatttca taggattaat cggataagca aaatcatctt
      181 tgaaatttgt tatactttct gtaatttgtt tcttgatttc tctaagggta tcgggtattt
      241 caaaataagg tatatctata gaatttagat tatcattctt attgattttt ttcccctctt
      301 tctctttttt ttcttcttct acttcttttt ctttaatcaa ttcatgtaaa attgcatcta
      361 ttgtataacc tatatccgca gcaatctcaa tgtctggagg atagtatgtt tctacttccg
      421 cagaagtaaa atcgatatga ataatttttt tgtctagatt tctattccaa ttttttgggc
      481 tatattcgac tagatcatat cctatcgcaa taaccaaatc agattctctc aatgcaatga
      541 ggaggttatc cgtattttgg attccaattg tttgtaaatg tctttcagat ttatctgata
      601 ttacaccttt tgccatgaat gtattcattg aaaatatacc tgttttttct acaaatcttc
      661 ttatgtttga gctagcgctc cctcttatac agccgtttcc taccaaaatt ataggaattt
      721 tggcactcaa tatcatttta actgcttttt ttatcaaacc cttttgtggt ctagatctta
      781 atgttcgttt aggctttttt attggtttaa tatcagattt ttctttggct atatcctctg
      841 gcagttctag atgtactgca ccagcttttt cttcaattga aattttaaaa gctcttctta
      901 ctaattctgc aataacatca gtattgtgga ttgaccatct ccattttgtt ataggataga
      961 acatagatac aacgtccata ttttgatgag attctttatg acggaggtta gaatctgttt
     1021 gtccggtaat agccaatata ggggatctgt ccatatttgc atttgccacc cctgttatca
     1081 aatttgttgc accaggaccc aatgtagata gacacactcc aactttttgt gttagtctac
     1141 catagacatc agccataaaa gcagctcctt gttcatgtct aactaagata aacttaattt
     1201 ttgaatttaa taacgaaaac attaaatcat tattttcttc accaggtaaa ccaaatatat
     1261 attctactcc ttcagattct aagcatctta gaaatagatc ggatgccttc atcattattt
     1321 gactatacta ttgtataaca tagtatttca ttattgatta tctaataaaa gattagattc
     1381 atctagaatt gtcttagtat atataaaatc taattcaaac tagtattatt ttatttctat
     1441 tttctattgt attataaaga aaaaaaattc gttattttgg ggtatcataa cattaatcat
     1501 aatattgttc ttacatataa taagtcaaag gttaaatgac aatttaaact ctaaaagtta
     1561 tgcagaaata tatattcatt taatatattt tatac
//
LOCUS       DATAFW010000174         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6318649, whole genome shotgun sequence.
ACCESSION   DATAFW010000174 DATAFW010000000
VERSION     DATAFW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6318649"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VHJ57_03990"
     CDS             <1..183
                     /locus_tag="VHJ57_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX2487725.1"
                     /translation="NPDFVKLAKSFGAIGYHVKSTEEFPKILEKAKESTSVPVIIAID
                     VDYSKNQLLLNADFND"
     gene            complement(294..425)
                     /locus_tag="VHJ57_03995"
     CDS             complement(294..425)
                     /locus_tag="VHJ57_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487726.1"
                     /translation="MWGSLGCKDDQFLIPHDIAIDSEGKIYVTDSGKSHFRTNYDCN"
     gene            1133..1987
                     /locus_tag="VHJ57_04000"
     CDS             1133..1987
                     /locus_tag="VHJ57_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX2487727.1"
                     /translation="MRYKEAIDKKDIIKILNGCSDLRLKTYVMLLASTGLRATEALSI
                     RLKDLDTTDSNNNSSPAKLIIRGQYTKTKVDRYVFLTREVVEQSKIWLDFKYRKRRIC
                     HIDKETGKSISEYRTPEKKQNELIFSINRTDTPKPEILYNNLAAIFAKTLDRTGMGSR
                     EDGNENRREITLHSFRRFVKTTISDLGYADYSEWFIGHSGSTYWRKKDNEKAEIYKKI
                     EPYLTFLNIQQLNRQGADLETKIEELQDINQVLREKDKMKKDVIANLSDKLIMLSERL
                     DALEKFNR"
     gene            complement(2266..>2506)
                     /locus_tag="VHJ57_04005"
     CDS             complement(2266..>2506)
                     /locus_tag="VHJ57_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487728.1"
                     /translation="ERQFKHVRKLIITQTKIWVIEITWNELIWQIWLVIWSHYGNGLN
                     DVMGFFIRIGFEWATFYIFFPRLESCYHHMLKKIF"
BASE COUNT          989 a          342 c          354 g          821 t
ORIGIN      
        1 aatcctgact ttgttaaact tgccaaaagt tttggagcca ttggatatca tgttaaatct
       61 actgaagaat ttcctaaaat cttggaaaaa gcaaaagaat caacatctgt cccagtaata
      121 atagcaattg atgtagatta ttctaagaac caacttttat taaatgctga ctttaatgat
      181 tagatattgt tattattatg atcacttatt atcggttatg aaaaaccaaa aataatataa
      241 gaaaaattaa tctaatttgt tcaatattgt tcgtatactt tatgattagc tccttaattg
      301 caatcataat ttgttctaaa atgtgatttt ccgctatctg taacgtagat tttaccttca
      361 gaatctattg caatatcatg tggaattagg aattgatcat ctttacatcc aagtgatccc
      421 cacattgtga taaaatttcc atctttatca aatttctgga tattgcaatt ttctgcatcg
      481 ctaacataga catcatcttg tgaatcaata gttattccat gagcatggag aaaatgtcca
      541 tctccagatc caacagtacc ccaactccct attagatttc catttctatc aaattttttt
      601 ataaagtgga aattagaaga ataataagaa tcagacccta gaacctaaat ccttgttagc
      661 gtataaaaca gattcaattt ggttatttat ggtgcattaa atatgataaa tacaatatct
      721 ataaaggaaa ttttcaaata actattagat gaataaaaat attcaagtta ttacaaagag
      781 taaccaattt gatattcaat cttctgattt attgaaaaaa tttatacaaa gtgttactat
      841 cagaaataag agaacagcag ttcaatataa ttttcgactt tcattttttg agaggtttct
      901 aaatgaaaaa tataaaatta acttggacgt gctcttacaa aaaataaaat taacataata
      961 caatctctat gatgttttaa acgattattg cctattttta cagaataact ataatatagg
     1021 aacattagca tttagagata agataataac tgtaaagact tttcttgaat ataatgatat
     1081 agaaataagt ccaagaaaat tcaaactaaa agtaagattt ccaaaaactg tattgaggta
     1141 taaggaagcc atagataaaa aagatatcat taaaatttta aatggatgtt ctgacttgag
     1201 attaaaaaca tatgtaatgt tattggcatc taccggactt agagcaacag aagcattatc
     1261 tattcgttta aaagatttgg atactactga ttctaataac aatagtagtc ctgcgaaatt
     1321 aatcattaga ggacaataca caaaaacaaa agttgataga tatgtatttt tgactagaga
     1381 agtagtagag caatcaaaaa tatggttaga tttcaaatat agaaaacgaa gaatttgtca
     1441 tatagataaa gaaacaggaa agagtatatc tgaatataga actccagaaa agaaacagaa
     1501 tgaattaatt ttttccataa atcgaacgga cactccaaaa ccagaaatat tatataacaa
     1561 tttggcagca atttttgcaa aaacattaga tagaactgga atgggttcac gagaggatgg
     1621 aaatgaaaat agaagagaaa taaccttaca ttcatttaga cgatttgtaa agactactat
     1681 ttccgacctc ggatatgcag attattctga atggtttatt ggccattctg gttctacata
     1741 ttggagaaaa aaagataatg aaaaagcaga gatatacaaa aagatagaac catatttgac
     1801 ttttctaaat attcaacaat taaatagaca aggagcagat ttagaaacga agattgaaga
     1861 gttacaggat ataaaccaag tattaagaga aaaagacaaa atgaaaaaag atgttattgc
     1921 aaatttatct gataaactga ttatgttatc agaaagatta gatgcattag aaaaatttaa
     1981 cagataagga taatttaatt cccatatcct gaacaagcaa gtaattagag tagaggattt
     2041 atagactaca tctaactacc tgtgtcgtcc agactatgac caacatgata tttttccata
     2101 tttgttgaga gaagtttgtt tttaagagat ggtatttcat tcataacgca tattgtattt
     2161 atcaactctt gtaaatctat gttgagactt tcatcaacaa tgaatttttt attacatcaa
     2221 aaataattta ttgttattag taagaaaaat aaaaaaataa gtatattaaa atattttctt
     2281 aagcatatga tgataacagc tttctaatct tgggaagaaa atgtaaaaag ttgcccactc
     2341 aaatccaatt cgtataaaga atcccataac atcattaagt ccattaccat agtgactcca
     2401 tattaccagc catatctgcc atatcaattc attccatgtt atttctatta cccagatttt
     2461 tgtttgtgta ataattaatt tcctaacatg tttgaactgt ctttct
//
LOCUS       DATAFW010000175         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_6322830, whole genome shotgun sequence.
ACCESSION   DATAFW010000175 DATAFW010000000
VERSION     DATAFW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6322830"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="VHJ57_04010"
     CDS             complement(<1..968)
                     /locus_tag="VHJ57_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487729.1"
                     /translation="MITNSPFKSSTSSFDHNNNLNLLEIPPIQAQDDGDDLRDDGGGD
                     EVAGFEKEDDDGGDENEEDMGQFNFESEDTEAQETEDAEEIEDESGQEKEDINEADQQ
                     EEFQNAQFDFESEDTEDQETEEVKEVEAQEDEGGNDDATQETGQFNFESEETDQQAIG
                     NDADQQVGQQGQQQETVGSDEIVEQIQQQKNAGTNVEEQAEQQGRQQVVDSNDISEQI
                     EQQAKQLGVGEDAAQQLGQATLEFGTDTDIEQQIEQQAKQLGVGEDAAQQLGQAATQI
                     EFDKQSIGQKAEPQQEAFSSDVEQKISQTATQIAKSSEDQGSEEPS"
     gene            complement(1686..1940)
                     /locus_tag="VHJ57_04015"
     CDS             complement(1686..1940)
                     /locus_tag="VHJ57_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEX2487730.1"
                     /translation="MSISSTLIGILPIAGLMSDQNLESVISHLTSVNQACSELGSNIV
                     KDPFMLLSFLALPVIPSLKLTYKGLVDVDKFQFTNLWVKD"
     gene            1965..2354
                     /locus_tag="VHJ57_04020"
     CDS             1965..2354
                     /locus_tag="VHJ57_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX2487731.1"
                     /translation="MQSKLTNVRDVMNKSIISIEKDDTLRLAVKKMTHGEFGSVIVTE
                     KGKPIGILTERDILKLIANETMDLENKAEIIMSTPLISIDSSASIAEAADVMLTNNIR
                     RLMVMENDKYVGIISQRELQRFIIESK"
     gene            2851..3255
                     /locus_tag="VHJ57_04025"
     CDS             2851..3255
                     /locus_tag="VHJ57_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487732.1"
                     /translation="MIDPKVKGIPEDADIEKRKIAKILLEQFKEFNSILYYFNNRDIY
                     FFEPFQDQLKLTATNFSFRDYYQVVNQTKKTYLRDAVLSKATTGRNLAVIATPVINKD
                     NNMTGILSGTINFNYYDKFLQSLNLQNNTRLY"
BASE COUNT         1096 a          546 c          464 g         1218 t
ORIGIN      
        1 tctgaaggct cttcgcttcc ttgatcctct gatgatttag caatttgtgt tgctgtttga
       61 ctaatttttt gttctacatc agaactaaat gcttcttgtt gtggttctgc cttttgtccg
      121 atactctgtt tatcaaattc tatttgtgta gctgcttgtc ctaactgctg tgctgcatct
      181 tcacctactc ctaactgttt agcttgttgt tcaatctgtt gttctatatc agtgtcagtc
      241 ccgaattcca aagttgcttg tcctaactgc tgtgctgcat cttcacctac tcctaactgt
      301 ttagcttgtt gttcaatctg ctccgatata tcatttgaat caacaacttg ttgtcttcct
      361 tgctgttctg cctgttcttc tacattggta cctgcatttt tttgttgttg aatctgttct
      421 actatttcat cactacctac agtttcttgt tgttgtcctt gctgacctac ctgctgatct
      481 gcatcgttac caatagcttg ttgatctgtt tcttctgatt caaagttaaa ttgaccagtc
      541 tcttgagttg catcatcgtt acctccttca tcctcttgtg cctctacctc tttaacctct
      601 tcagtctctt ggtcttcagt gtcttctgat tcaaagtcaa actgagcatt ctgaaattct
      661 tcctgttgat ctgcttcgtt tatgtcttct ttttcttgtc ctgattcgtc ctctatctct
      721 tcagcatctt ccgtttcttg agcttcagtg tcttctgatt caaagttaaa ttgacccatg
      781 tcttcctcat tttcgtctcc accatcatca tcctcttttt caaaaccagc aacctcatcc
      841 cctccaccat catcccttaa gtcatctcca tcgtcttggg cttgaattgg aggaatttct
      901 aaaagattga gattgttatt atggtcgaaa gaactggtag aacttttaaa aggagaatta
      961 gtaatcaaaa ttatactaag aaaactgatt aaacctatta ttactaataa atgcggtctt
     1021 ttcaaattta actgttaaat tatattgaat tatctaataa aatagtttaa aaagattgtt
     1081 taggtaaaaa aaaaagaaaa aaattatgca aaaattttat agtgtatcag tagataaatc
     1141 ttaaattatc tctttatcat tcttttatat ggcactggat aatcgtttat acttccataa
     1201 attggacata actatacttt ttatggatag tttttcaaat attctttgaa atataatctt
     1261 atttttagtt gccattttaa atatttttaa ttaaaattat tataattttc tatttctaat
     1321 gctgtaatag caaatagtta tttatttttc tatgtttgat tactttgata ataaactttt
     1381 aatattatgt taccgaacac tgctttaatg agagttcgaa gccctctgag ctttgaattt
     1441 tagttattta gcctatttag ttagtgagaa ctaagcaaat atgaatccgt tttaatatct
     1501 ttaatacttt atgcttttag catttgatgc acatttatta aagaaaaata atatgcaaat
     1561 aaattcattt aatgttacct tatgatatta gattatcaaa tctaaatcat aaatatttat
     1621 aatattggca aaaaatataa ttctgaaacc atttgaacaa attacttgaa taaatggatt
     1681 tatctttaat cttttaccca aagattagta aattgaaatt tatcaacatc tactaatcct
     1741 ttgtatgtaa gttttaaaga aggaataaca ggcaaggcta gaaaggaaag taacatgaat
     1801 ggatctttta ctatgttact acctagctct gaacaagctt gattaacact tgtaagatgt
     1861 gaaattactg attctaaatt ctggtcagac attagccctg caataggtag tatgccaatc
     1921 agtgtagaag atatagatat attactttta agataaacct agtcatgcaa tccaagttaa
     1981 caaatgtaag agatgtaatg aataaaagta ttatttctat agaaaaagac gacactctga
     2041 gattagcagt aaagaagatg acacatggtg aatttggctc tgttatagtc acagaaaaag
     2101 gtaaaccaat aggaattcta actgagaggg atatcctaaa actcatagct aatgaaacaa
     2161 tggatctaga aaataaggcg gagatcataa tgagtactcc cttgatttcg atagattcat
     2221 ctgcaagtat tgccgaggca gcagatgtta tgcttacgaa caatatccgt cgtttgatgg
     2281 ttatggagaa cgataaatat gtaggaataa tttctcagcg tgaactccag agatttataa
     2341 tagaatcaaa atagtttttt ttcttagtta ataattgaat ccaatttcaa ataagttacc
     2401 aaattaataa ataatagttt gccgatccca attacatttc atgctcaaag ccctcataac
     2461 ccttattact atactaaatg ttttcaacaa tatcctcttt ctaataagat aatataccaa
     2521 atactaaatt ttcaagtcat agatagaatt atattaaatt gttcttacaa ttctcaagat
     2581 tagataatgt taaagatttt aagttttttc attggtatta ttactatcag cagtagtatt
     2641 atcttatttt ctgcaaactc actttctatc tcttctattt atgctcaaga tactcaatac
     2701 tagaataaca caaatgaaaa taataattct ttacttttac atgaactcaa aaatataaaa
     2761 ataatacttg agagtaaagt aacaaaacta gttacagctc tacaaattgc tagtaatcta
     2821 ccagaaattt tacagcctcc tgatataaat ttgatagacc caaaagttaa gggtatacca
     2881 gaagatgccg atattgagaa gagaaaaata gctaaaatac tattggaaca atttaaagaa
     2941 tttaattcaa ttttatatta ttttaataat agagatatat attttttcga accatttcaa
     3001 gatcaattaa aattaactgc aactaatttt tcatttcgag attattatca agtagtgaat
     3061 cagacaaaaa aaacttactt gagggatgca gttctctcta aagcaacaac aggaagaaat
     3121 ctggcagtaa ttgctactcc cgtaataaat aaagataata atatgacagg aatactgtca
     3181 ggcacaatta attttaacta ttatgataaa tttttgcagt cacttaattt acaaaataat
     3241 accagactgt attaattgac aagaatggag taaagattgg tgattcgaat ggaaatgaga
     3301 cgtcagtctc aaaagaatct tttg
//
LOCUS       DATAFW010000176         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_5973656, whole genome shotgun sequence.
ACCESSION   DATAFW010000176 DATAFW010000000
VERSION     DATAFW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5973656"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VHJ57_04030"
     CDS             complement(<1..198)
                     /locus_tag="VHJ57_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487733.1"
                     /translation="MKKEINTKNKDFLTTLFKEYYFKNLGLLNPPIQMPNREFGFMTF
                     DGKVIRHLSFSKLGSLKAYIIQ"
     gene            complement(195..1208)
                     /locus_tag="VHJ57_04035"
     CDS             complement(195..1208)
                     /locus_tag="VHJ57_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487734.1"
                     /translation="MSIILGKDDLAKYPFLEEAGTYVRESHFDLDDFNRSEFLHIIER
                     ASKRIEFALIQGKVFMDLEKYDIELFTFLVSLIMMKYIYEDSAIKKFSLFEAIRVERF
                     LISDLLKEKDLRKKNLLISRIFRELFQLDVDLDTKKNNLFKMNISNYLDRATQFNEIE
                     WKLINRAVDNGYVYLDVDETVRLIRYEIYKLMYNKIKNMNITKIPEQIRFHATLIKNK
                     LIPTIKYTKNTTDLPPCIKKALEFLNKSENLPHSARFMLATYMLSLGKSIDEVVSLFQ
                     NAPDFNEKITRYQVEHLAGKKGSQTKYSVPACSKLVNENLCYATIDCKGINNPIQFGR
                     NRK"
     gene            1294..2190
                     /locus_tag="VHJ57_04040"
     CDS             1294..2190
                     /locus_tag="VHJ57_04040"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX2487735.1"
                     /translation="MKIGFHVSITGSLSNSIKNAEEIGCTAFQIFTRNPRGWIPKHLE
                     RKDVDEFKSKLENSYIDKKNVVVHMPYLPNFAAPESESYYKSKKVLSEEIIRCYLLDI
                     SYLILHLGSHLGFGEKNGIKQIVNACNFALDNFNSLYRRHLNLNILIENSAGQKNSIG
                     SKFDEIKDILDELNNKKFGVCFDSCHAFASGYDLRTSNKVIETIDNFNDIINIKNLKV
                     LHLNDSKGKINENIDRHEHIGLGSIGKEGLIEFLKHKYFQNIPIIMETPNDKTRGDKE
                     NMKVILDYIEDEKIELNIPRKI"
BASE COUNT          837 a          309 c          322 g          791 t
ORIGIN      
        1 ctggatgata tatgctttta aacttccgag ttttgaaaac gataaatgcc taattacttt
       61 gccatcaaat gtcataaaac caaactcacg attaggcatt tgaattggag ggttaagaag
      121 cccaaggttt ttaaaataat attctttgaa aagtgtagtt aaaaaatctt tattttttgt
      181 attaatttct tttttcattt tctatttcta ccaaattgta taggattatt gattccttta
      241 caatcgatag ttgcatagca aagattttca tttaccagtt tggaacatgc agggacagag
      301 tattttgttt gactaccttt tttacctgct aaatgttcaa cttgatacct tgtaattttt
      361 tcattaaaat ctggagcgtt ttgaaatagt gaaactactt catcgatact cttacctaat
      421 gaaagcatat aagtagctaa cataaatctt gcagaatgag gtaagttttc acttttattt
      481 aggaattcca acgctttttt tatacaagga ggaagatcag ttgtattttt tgtatattta
      541 atggtaggaa ttaatttatt tttaatcaaa gtagcgtgaa atctaatttg ttctggtatt
      601 ttagtaatat tcatattttt aattttatta tacatcaatt tgtaaatttc ataccttatc
      661 aatctgactg tttcatctac atctaagtat acataaccat tatcaacagc tctattgatt
      721 aatttccatt cgatttcatt aaattgggta gctctatcta aataattaga aatattcatt
      781 ttaaataagt tatttttttt agtatctaaa tctacatcta actgaaaaag ttctctgaaa
      841 atcctagaaa taagaagatt ttttttcctt agatctttct cttttaagag atcagatatt
      901 aaaaacctct cgaccctaat agcttcaaat agtgaaaatt ttttgattgc tgaatcttca
      961 tatatatatt tcatcataat aagagaaacg agaaaggtaa atagttctat atcatatttt
     1021 tcaagatcca taaagacctt tccttgtatc aaagcaaatt caatcctttt agaagctctt
     1081 tcaatgatat gcagaaattc tgatcggtta aaatcatcta aatcaaaatg actttctcga
     1141 acataggttc cagcctcctc taaaaaagga tattttgcaa ggtcatcttt gcctaaaatg
     1201 atactcaata ctattactat tattcattac caaaataaac ctacttcgta aagatattat
     1261 cgaagtctaa acaaaaaaat ttagatatca aaattgaaaa taggtttcca tgtatctatt
     1321 acaggctcac taagcaactc aataaaaaat gcagaagaga ttggctgtac tgcttttcaa
     1381 atatttactc gaaatccccg tggttggata cccaagcatt tggaaaggaa agatgttgac
     1441 gaatttaaaa gtaagttaga aaatagttat atcgataaaa aaaatgttgt tgttcatatg
     1501 ccatatttac ctaattttgc cgcaccagag agcgaatcat attataaatc caaaaaagtt
     1561 ctctcagaag agattattag atgttatcta ttagatattt cttatctaat cctgcactta
     1621 ggtagccacc ttggattcgg ggaaaaaaat gggataaaac agatagttaa tgcatgcaat
     1681 tttgctcttg acaattttaa ttctttatat agaaggcatt taaatttaaa tattttaatt
     1741 gaaaatagtg ctggtcaaaa aaatagcata ggtagtaaat ttgatgaaat taaagatata
     1801 ttggatgaac taaataacaa aaaatttggg gtttgttttg attcttgcca tgcatttgct
     1861 tcgggatacg atctaagaac atctaacaaa gtaatagaaa caatagataa ttttaatgat
     1921 atcataaata ttaaaaattt aaaagtcttg catttgaacg actctaaagg taaaatcaat
     1981 gagaacatag atagacacga acatattgga ctaggttcaa taggtaaaga aggactgata
     2041 gaatttttaa aacataaata ttttcaaaat attcccataa taatggaaac gccaaatgat
     2101 aaaactagag gagataaaga aaacatgaaa gttatattag actatattga agatgaaaaa
     2161 attgaactga atataccaag aaaaatatag aaccttataa tcatggataa tatttatggt
     2221 tagttacgat agaattgcga aactctcttt agaaagagg
//
LOCUS       DATAFW010000177         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_513269, whole genome shotgun sequence.
ACCESSION   DATAFW010000177 DATAFW010000000
VERSION     DATAFW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513269"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..984)
                     /locus_tag="VHJ57_04045"
     CDS             complement(253..984)
                     /locus_tag="VHJ57_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HEX2487736.1"
                     /translation="MSFRIVVDERERNSRVPKFLIEDGAQIDFARLSVGDYIIAPEIA
                     IERKTIHDLINSIYDGRLFIQCSDLTQFYSKPILIIEGNILNLQFENSSESQMNEIEI
                     SFEKILQVYDALATIALIFRIPVIHSPSANYTSKFLIILLNKAIKSGITINGPLIKRI
                     KKGNHMQLQQLSILSSLPGVGDKLAIKMLKKFKTPTRAMNASVSELALIPGFGKARAE
                     KVKKILNYEFNEDITKNQQETLNFE"
     gene            complement(1046..1417)
                     /locus_tag="VHJ57_04050"
     CDS             complement(1046..1417)
                     /locus_tag="VHJ57_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HEX2487737.1"
                     /translation="MSQENELPPWLKEQISRLQQLQQNLQAIMMQKQQIELEIVETER
                     ALEELSKTTTSDSIYKAAGPLLIKSEKDTIEKELTEKKELANTRVMVLGKQELRVKEN
                     LKEVENKINQMLKGTQSTTQK"
     gene            complement(1458..1736)
                     /locus_tag="VHJ57_04055"
     CDS             complement(1458..1736)
                     /locus_tag="VHJ57_04055"
                     /inference="COORDINATES: protein motif:HMM:NF020899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HEX2487738.1"
                     /translation="MRNYKAKINFSLDLSLPSETKNFLESLYNALAADTKILVTPQIK
                     LYILNSDCMSQLIIEISTSDLAELRSAINSYLRLIKTTHLCITNLLFV"
     gene            complement(1717..1956)
                     /locus_tag="VHJ57_04060"
     CDS             complement(1717..1956)
                     /locus_tag="VHJ57_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37"
                     /protein_id="HEX2487739.1"
                     /translation="MVNRNRKGQTVLKGLGTKFGATVRKRYSKVYRTLKQKRRCPSCG
                     SLKFMRIACGIWNCPKCNFKVAAGAYDINIEKLQS"
     gene            complement(1956..2774)
                     /gene="rrp42"
                     /locus_tag="VHJ57_04065"
     CDS             complement(1956..2774)
                     /gene="rrp42"
                     /locus_tag="VHJ57_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000178 - exosome (RNase complex)
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex protein Rrp42"
                     /protein_id="HEX2487740.1"
                     /translation="MSLSKKSPIVIEQLRKRQMLDLITNGKRLDGRSLEDIRDLEIEI
                     DIIKKADGSAKVRLGNSEVIAGVKVETGAPFEGLENKGALIVTAEVLPIASPYIEPGP
                     PDEETIELARVVDRGIRESQMIDLDKLVLIPGKIVYVIFVDCSIINADGNLFDATSYA
                     VVAALLSSKLPIFEIHDEKVVDTGKKQEPPITTIPVSITSIRIGNNIIIDPTAEEEAC
                     MESRITITTNSNNEYTAIQKGSTGYFTLEQIKFSAQTARVKAETVRTKLKELTK"
     gene            complement(2771..3532)
                     /gene="rrp41"
                     /locus_tag="VHJ57_04070"
     CDS             complement(2771..3532)
                     /gene="rrp41"
                     /locus_tag="VHJ57_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosome complex exonuclease Rrp41"
                     /protein_id="HEX2487741.1"
                     /translation="MDQTILIDENGKRTDGRSIDDLRDVKITVGVLKNADGSASIEFG
                     KNKIIVAVYGPREVHPKHMALPDRCVLRCRYHMSPFSTDTRKNPAPSRREVEISKVMR
                     EALEPAIMLEDYPRAAIDIFVEVLQADGGSRCAGIAAASVALADAGVNMRDLVSACAA
                     GKVNDKIVLDINDIEDKEGSADMPVAYLPNLEQITLLQLDGILTESQFNECIEKAIHG
                     CKMVYEIQRKALIEKYFGNDLPEGEEKELEENKEN"
     gene            complement(3551..>3869)
                     /locus_tag="VHJ57_04075"
     CDS             complement(3551..>3869)
                     /locus_tag="VHJ57_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487742.1"
                     /translation="ATQTRILIGQNGILVVTGKNDEGIALAFKAIKLVEEEAHTSNLT
                     QKVKDLLNVSEDAAQTETNNSSVNNEDYVNNSNTDNNSKDMANDHSSNITTTESPKEV
                     QPN"
BASE COUNT         1171 a          657 c          510 g         1531 t
ORIGIN      
        1 tttttcagct tgaacataca caaaattcat cggtattatc tggggaaagc cattgatatc
       61 tatagttgca aatcttccaa ctgcttgatt atttagaaaa ttaataatat tttctttgga
      121 ttgaatttca agttttttta taagtttcat tagaatttat ttcataataa ctttataaag
      181 agatttaata taaaatgaag aatttatatt ctaaattttg aaatatattc caatacaata
      241 aattcaatta atttattcaa aatttaatgt ttcctgttga ttttttgtaa tatcctcgtt
      301 aaattcataa ttaagaattt ttttcacttt ttcagctctt gcttttccaa aaccgggaat
      361 caaagctaat tcagataccg atgcattcat tgctcttgtt ggtgttttga attttttaag
      421 catttttatg gctaatttat ctcctacacc aggtaatgat gaaagtatag ataactgttg
      481 taattgcata tgatttcctt tttttattct ctttattaat ggtccattga ttgtaatacc
      541 agattttatt gctttattca ataaaataat taaaaatttt gaagtgtaat ttgccgaagg
      601 agaatgaata actggtatcc taaaaattaa tgcaatagtt gcaagagcat cataaacctg
      661 caaaattttc tcaaatgaaa tttcaatttc attcatttgg ctttcactag aattttcaaa
      721 ctgtagattc aaaatatttc cttctattat taaaataggt ttagaataaa actgggtcaa
      781 atcggaacat tgaataaaaa gtcttccatc atatattgaa ttaattaaat catgtattgt
      841 ctttctttct atagctattt ctggtgctat aatataatct cctactgaaa gtcgagcaaa
      901 atctatttgt gcaccatctt caattaagaa tttaggaact ctactatttc gctctctttc
      961 atctacaaca atacgaaaag acacaaatag aattaaatat tcggattatt gtttctaact
     1021 aaaaattaaa ctataaaaaa taactttatt tttgtgttgt agattgtgtt ccttttagca
     1081 tttgattaat cttattttcg acttccttaa gattttcttt tacacgtaat tcttgttttc
     1141 ctaataccat aactcttgta tttgctaatt cttttttttc ggtaagttcc ttttctattg
     1201 tatctttctc agattttatt aataaaggtc ctgctgcttt ataaatagaa tcagatgtag
     1261 ttgttttact taattcctcc aatgcccttt ctgtttcaac aatttctagt tcaatttgtt
     1321 gcttctgcat cataatagct tgcaggtttt gttgaagctg ttgtaatcta gatatttgtt
     1381 ctttaagcca aggaggtagt tcattttctt gactcaattc attgaatagt taagtaattg
     1441 aatataaatt cataacgtta tacaaatagc agattagtta tacataaatg agtggtttta
     1501 ataaggcgta agtaagaatt gatagcagat cttaattctg ccaaatcaga agtagaaatt
     1561 tcaattatta actgtgacat acaatctgaa tttaatatat ataatttaat ttggggagtg
     1621 actaagattt ttgtatcagc tgccaatgca ttatataaag attctaaaaa attttttgtt
     1681 tctgaaggta aagagagatc aagagaaaaa ttaattttag ctttgtagtt tctcaatatt
     1741 tatatcatat gctccagcag ccactttaaa attacatttt ggacaattcc aaattccaca
     1801 tgcaattcgc ataaatttta atgaaccaca ggaaggacaa cgtctttttt gtttaagagt
     1861 tcgatatact ttactatatc tttttctaac agtagcacca aattttgttc ctagaccctt
     1921 tagtacggtt tgaccttttc tgtttctatt taccactatt ttgttagctc ctttaattta
     1981 gttcgtacag tctctgcttt aactcttgct gtttgagcag aaaattttat ttgttcaaga
     2041 gtaaaataac cagttgatcc cttctgtatt gcagtatatt cattattaga attagtagtt
     2101 attgttattc ttgattccat acatgcctcc tcttcagcag taggatctat aataatatta
     2161 tttcctattc gtattgaagt tattgataca ggtattgttg taattggagg ttcctgtttt
     2221 tttcctgtat caaccacctt ttcatcgtga atttcaaaaa ttggtagttt actgcttaat
     2281 aatgcagcaa ctacagcata tgaagttgca tcaaaaagat tgccatcagc attgattata
     2341 ctacaatcaa cgaaaataac ataaactatt ttaccaggaa ttaaaactaa tttatctaag
     2401 tctatcatct gagattctct gatgcctcga tcaactactc tagcaagctc tattgtctcc
     2461 tcatcaggag gtccaggttc tatgtaggga gatgcaatcg gtaaaacttc tgcagttaca
     2521 attaatgcac ctttattttc taatccttca aatggtgcgc cagtttcaac ttttacacct
     2581 gcaattactt ctgaattacc taatcttacc ttagcagacc catctgcttt ttttattata
     2641 tctatttcta tttctaaatc tcttatatct tctaaacttc taccatcaag tctttttcca
     2701 ttagtaatta aatcgagcat ctgtcgttta cgtaattgtt ctattactat tggtgatttt
     2761 tttgataaac tcaattctcc ttattctcct ctaattcttt ctcttcgcct tcaggcaaat
     2821 catttccaaa gtatttttct attaatgctt ttctttgtat ttcataaacc attttacatc
     2881 catgaatagc tttttctata cattcattaa attgagattc agttaatatt ccatctaatt
     2941 gtaataaggt tatctgttca agatttggta aatatgcaac gggcatatct gctgatcctt
     3001 ctttatcttc aatgtcattg atatcaagaa ctattttatc atttactttt ccagcagcac
     3061 atgctgacac taagtctcgc atattaactc cagcatcagc taaagctact gatgcagcgg
     3121 caattcctgc acatctggag ccaccatctg cctgtaatac ttcaacaaaa atatcaatag
     3181 cagctctagg ataatcctct aacattattg ctggttctaa tgcttcccgc attacctttg
     3241 aaatttcaac ttctcttctt gagggagctg ggttttttct agtatctgtg gaaaaaggtg
     3301 acatatgata tctacatctt aagacacatc gatctggaag agccatatgt ttaggatgaa
     3361 cttcccttgg cccatatact gctactataa ttttgttttt accaaattct atagaggcag
     3421 atccatctgc attctttaag actcctacag taatcttaac atctcttaaa tcatcaatag
     3481 atcggccgtc agtacgcttt ccattttcat ctattagaat tgtttgatcc ataatcttta
     3541 tcctctattc ttaattaggt tgaacctctt ttggagattc agtagtagta atatttgaac
     3601 tatgatcatt tgccatatct ttggaattgt tatctgtatt actattgttg acatagtctt
     3661 cgttattcac agaactatta ttagtttctg tttgtgctgc atcctcagaa acatttagca
     3721 aatcttttac tttttgtgtt aaatttgagg tatgtgcttc ttcctcaact aattttatag
     3781 ctttaaaagc aagagcaata ccttcatcat tttttccagt tacaacaagt atcccatttt
     3841 gcccaattaa aattctagtt tgtgtagct
//
LOCUS       DATAFW010000178         4392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Nitrososphaeraceae archaeon isolate SMAG_U6813
            k141_695528, whole genome shotgun sequence.
ACCESSION   DATAFW010000178 DATAFW010000000
VERSION     DATAFW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955434
            Sequence Read Archive: SRR12681510
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrososphaeraceae archaeon (soil metagenome)
  ORGANISM  Nitrososphaeraceae archaeon
            Archaea; Nitrososphaerota; Nitrososphaeria; Nitrososphaerales;
            Nitrososphaeraceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 02:21:58
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 819
            CDSs (total)                      :: 799
            Genes (coding)                    :: 797
            CDSs (with protein)               :: 797
            Genes (RNA)                       :: 20
            rRNAs                             :: 1 (23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Nitrososphaeraceae archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695528"
                     /isolate="SMAG_U6813"
                     /isolation_source="soil"
                     /db_xref="taxon:2169593"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.0127 N 93.7445 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VHJ57_04080"
     CDS             <1..1530
                     /locus_tag="VHJ57_04080"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX2487743.1"
                     /translation="GALMSRYSRSDKSMRRIFLEEFANNPNRGDEFYKRVLLEYGDDS
                     VAELGEAQVAIEYVSNIAAKKIEDQRIGLSFLEKSSRYVSFDKKNNGQYKYLKEKKIM
                     NSRYADAYIEACDYAFDLYSNNIESMQRFIIEREPIDNFQFYDSISKKDLQFNNLKES
                     ADIKLAQKIYKSTIKAKSLDLLRGLLPASTLTNLGITGNGRSFEYLLTRLYASNITEM
                     NALADQLYEELNLIIPSFVYRAKNPHGISMQNYLRNTKDVILKMAKEHLSQIKINPNL
                     ECVNLIFNEDNTEAEVKIASSILFEQAAGHTLTQIIEYVRSLPIEDRHKIIKTYTSLR
                     TNRRQRPGRAYEMTEYTFEMLTNFGMFRDLHRHRILTLERQLLSTRHGYDVPKEICDL
                     GILNDFKDCMYKCNEVFQLLVNDNYPEEAQYVVNFAYKYPFFMKLNLREATHMIELRT
                     LSQGHPDYRYVCQEIFKKIRNIHPILSEGIRFVDLKDYGLERLSSEKNIEKKKRNLID
                     N"
     gene            complement(1597..2619)
                     /gene="fen"
                     /locus_tag="VHJ57_04085"
     CDS             complement(1597..2619)
                     /gene="fen"
                     /locus_tag="VHJ57_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HEX2487744.1"
                     /translation="MGIDLKPILTSCSIKPSDLSDKIIAVDAYNTIYQFLATIRGPTG
                     DPLTNNKGEITSHLSGLFYRNINFLTENIKLLYIFDGKPHSLKFNEIKRRKESKQEAI
                     EKYQEALEEGRLEDAKKYSQGTVILTDEIIEQSKIILKMLGIPVIHAPSEGEATASIL
                     TKDGTVYACASQDYDSLLFGAKRLIRNLTITGKRKIPNKNKYVNIEPEIIHQSQILES
                     TKLTLEQLVDVGILIGTDFNPGGLPGIGPKTALKLIRENGRLEEIEKIKDNLKDVPYQ
                     QIREIFLNPENISIGNIEFDNPNNKEIIGFLCDTMDFSKERVESSLEKVKKSQEKRSQ
                     SLERWF"
     gene            2783..3115
                     /locus_tag="VHJ57_04090"
     CDS             2783..3115
                     /locus_tag="VHJ57_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487745.1"
                     /translation="MNTNEKLVLFRSIIIMILFLSMFYLAPIIAQNSTSITESHLIGN
                     CQAFTKLQNEKLNLEKKLNETKQNFLPNLIQEKSLTLKLDDLNSKLALLTDNRYCSEL
                     EKRITDQN"
     gene            3370..>4392
                     /locus_tag="VHJ57_04095"
     CDS             3370..>4392
                     /locus_tag="VHJ57_04095"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX2487746.1"
                     /translation="MKDNIHVVFWNLGNLFDIQPNEISSNLEFTPNKGWNEKVRDIKI
                     QNLTNIIKSTFDTGPDLIGVCEIENAFLAEKLIDELKKSLGRDDYQIAGTNIDGKLKP
                     YHKGPDLRGIDTCLIYSNKIFEQLDGHGYMINFRFPTRDIFYAKFRVLKNNAKLNVLV
                     NHWPSRRGRDDYSELMDTEYSRYMAAEHCGRIVDSILKYPKMEIEKFPNNVDSIYEGK
                     TVLEKLEERWDINILVMGDFNDEPFDKSIMKYLYATPDKNLLRKWNRIFRLAKEDFKN
                     RDKTYKQIYLEQPSVLFNCMWKLYPNGSLYFRKTNSFNLFDQFIISRGLLCGKQGLKM
                     NLNSINI"
BASE COUNT         1651 a          564 c          618 g         1559 t
ORIGIN      
        1 ggggcactaa tgtcaagata ttcacgatcc gacaaaagta tgcgtagaat atttttagaa
       61 gaatttgcaa ataatcctaa tcgtggagat gaattttata aaagagtatt attagaatat
      121 ggtgatgatt ctgttgcaga attgggagaa gcacaggtgg caatagaata tgtatccaat
      181 attgcagcca aaaaaataga agaccaaaga ataggtttat cttttctaga aaaatcatca
      241 agatatgttt catttgataa gaaaaataat ggccaatata aatatttgaa agaaaaaaaa
      301 ataatgaatt ctagatatgc agatgcatat atagaagcat gtgactatgc ttttgacctt
      361 tattctaaca acatagaatc tatgcaaaga ttcattatag aaagagaacc tatagataac
      421 tttcaatttt atgattctat ttctaaaaaa gatctacaat ttaataattt aaaagaatcc
      481 gctgatataa aattagctca aaaaatatat aagagtacta taaaagctaa atcattagat
      541 cttttacggg ggcttttacc cgcgtctact cttactaatt taggtattac tggtaatggg
      601 agatcttttg aatatctttt aacaagattg tatgcatcga atataacaga gatgaatgcc
      661 ttagctgatc aattgtatga agagcttaat ctaattattc cgtcttttgt ttatagggca
      721 aaaaatccac acggaatatc tatgcaaaat tatttaagaa atacaaagga tgttattctc
      781 aaaatggcta aagaacatct ttcacaaatc aaaatcaatc caaatttaga atgtgtaaat
      841 ttaatattta atgaagataa tactgaagca gaagtaaaaa ttgcatcatc tattttattt
      901 gaacaagcag caggacatac acttacacag attatagaat atgttcgatc tttaccaata
      961 gaagatagac acaaaataat taagacatat acaagtttac gaactaatag aagacaacgt
     1021 ccaggtagag cttatgaaat gactgaatat acatttgaaa tgcttactaa ttttggaatg
     1081 ttccgtgatt tacatcgaca tagaatatta actttggaaa gacaattact ttctactaga
     1141 catggatatg acgtgcctaa ggaaatttgt gatcttggaa ttttgaatga tttcaaagat
     1201 tgtatgtata aatgtaatga ggtatttcaa cttcttgtta atgacaatta tccagaagaa
     1261 gcgcaatatg tagtcaattt tgcatataaa tatccatttt ttatgaaatt gaatttacga
     1321 gaagctacgc atatgattga attgagaact ttatcacagg gacatcctga ttatcgttat
     1381 gtatgtcaag aaatttttaa aaaaataagg aatattcacc caatcttatc tgaaggaata
     1441 agatttgttg atcttaagga ttatggttta gaaagactat ctagtgaaaa aaatatagaa
     1501 aagaaaaaga gaaatctgat agacaattag ataacatttt ttaaattttt tgtgactatc
     1561 aattgtatat atgtgtgtat attttattat taatatttaa aaccaacgct ctaaagattg
     1621 gcttcttttc tcttgacttt tttttacttt ttctaatgat gattctactc gttcctttga
     1681 aaaatccatt gtatcacata gaaaacctat aatttcctta ttatttggat tatcaaattc
     1741 tatattacct atagaaatat tttctggatt aagaaatatt tctcttatct gttgatatgg
     1801 aacatctttt aaattatctt ttattttttc tatttcttcc aatctgccat tctctcttat
     1861 tagctttagt gcagttttag gacctatacc tggaaggcca ccaggattaa aatctgttcc
     1921 tattaggata cctacatcta ctaattgttc aagggtcaat tttgtggatt ccaaaatctg
     1981 tgattgatga ataatttctg gttctatatt tacatatttg tttttattag gaattttacg
     2041 ttttccggtt attgttagat tacgaataag tcttttagct ccaaaaagta aagaatcata
     2101 atcctgactt gcacaggcgt ataccgttcc atctttagtt aaaattgatg ctgttgcttc
     2161 tccctctgat ggtgcatgaa taacaggaat tcctaacatc tttaatatta tcttggattg
     2221 ttcaataatt tcatccgtta aaattactgt gccttgacta tattttttag catcttccaa
     2281 tctaccctct tctaatgctt cttgatattt ttctatagct tcttgtttag attcctttct
     2341 tcgttttatc tcattaaatt ttaatgaatg aggttttcca tcaaaaatat ataataattt
     2401 tatgttctct gttaagaaat taatatttct ataaaataaa ccacttaagt gacttgttat
     2461 ttctcctttg ttattagtaa gaggatctcc agtaggacca cgaatcgttg ctaaaaattg
     2521 ataaatcgta ttatatgcat ctactgcaat tatcttatct gataaatcag atggttttat
     2581 tgaacaagaa gttaaaattg gttttagatc tattcccatt tattatgtat atatatgtat
     2641 ttaatgtacc ttttattttt tcatattata ttattaaaat aatagaatgt gattactatc
     2701 atatcaaata aacatgactt gagaattatt atagatctgt ataataatga gaacatataa
     2761 ttcagaatag atagataaaa ttatgaatac aaatgaaaag ttagttctgt ttagatccat
     2821 tattattatg attcttttcc tttcaatgtt ttatcttgct ccaataatag ctcagaattc
     2881 tacttcaata acagaatcac atttaatcgg aaactgtcaa gcatttacta agctacaaaa
     2941 tgaaaaactt aacctcgaaa agaaactgaa tgaaaccaaa caaaattttc tacccaatct
     3001 aatacaagaa aaatctttaa ctttaaaatt agatgatctt aactctaagc ttgcattatt
     3061 aactgacaat agatattgta gcgaacttga aaagcgcata acagatcaaa actaattctt
     3121 tgataaattt taacattatt gttatgatta atataatttt taatcgacct tctataaata
     3181 taaatataaa taaaaaaatt gttaatctct atatattata tattttatat acaataaaat
     3241 aaattgaaaa tatctataaa gttattacat taaattaata ggagtgatta tatgtaaaat
     3301 taaaaacttc attcttaaaa tactgtctgt ctgtaaattt tatatattaa ataaaatctt
     3361 attattaaat tgaaagataa tattcatgtt gttttctgga atctaggaaa cttatttgat
     3421 attcaaccta atgaaatctc atcaaacctg gaatttactc caaataaagg ttggaatgaa
     3481 aaagttcgtg atataaagat acaaaattta acaaatataa ttaaaagtac tttcgatact
     3541 ggtccagatt tgataggtgt gtgtgaaatt gaaaatgcct ttttagctga aaaactaata
     3601 gatgaattaa aaaaatcatt aggtagagat gactatcaaa tagctggaac aaacatagat
     3661 ggtaaattaa aaccatatca taaaggtccg gatcttagag gaattgatac atgtttaatt
     3721 tattctaata agatctttga acaacttgat ggtcatgggt atatgataaa ttttagattt
     3781 cctacacgag atatttttta tgctaaattt agagtcttga aaaataatgc taaactaaat
     3841 gttttagtaa atcattggcc atcaagaaga ggtagagatg attattccga acttatggat
     3901 acagaatatt ctagatatat ggcagcagaa cattgtggaa gaatagttga ctctatttta
     3961 aaatatccaa aaatggaaat agaaaaattc ccaaataatg ttgattcaat ttatgaagga
     4021 aaaacagtat tagaaaaact tgaagaaaga tgggacataa atattctagt catgggtgac
     4081 tttaatgatg aaccatttga taaaagtatt atgaaatatc tgtatgcaac accagacaaa
     4141 aatttactac gtaaatggaa tagaatattt aggttagcaa aagaagattt taaaaataga
     4201 gataaaacct ataagcaaat ttacttagaa caaccatcag tcttatttaa ctgtatgtgg
     4261 aagctttatc ctaatggaag tttgtatttt cggaaaacaa attcatttaa tctatttgac
     4321 caatttataa tatcacgagg tttattatgt ggaaaacagg gattaaaaat gaaccttaat
     4381 tctattaata ta
//
